LOCUS       DAWFAN010000001        56517 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWFAN010000001 DAWFAN010000000
VERSION     DAWFAN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 56517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56517
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.001"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            52..1005
                     /locus_tag="WGO65_000001"
     CDS             52..1005
                     /locus_tag="WGO65_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ0326373.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDEMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1122..1445)
                     /locus_tag="WGO65_000002"
     CDS             complement(1122..1445)
                     /locus_tag="WGO65_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326374.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1618..2979)
                     /locus_tag="WGO65_000003"
     CDS             complement(1618..2979)
                     /locus_tag="WGO65_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ0326375.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAAHRLVMIGDAAERYQEDPVRILRAIRLSGKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAADRHEKNEGQAKKRRRRRRKPKPKVEGT
                     DWE"
     gene            complement(3334..5034)
                     /gene="recJ"
                     /locus_tag="WGO65_000004"
     CDS             complement(3334..5034)
                     /gene="recJ"
                     /locus_tag="WGO65_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ0326376.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQTLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLMALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVSLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDTEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPARDITLEQAQNLAKHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5103..5819)
                     /locus_tag="WGO65_000005"
     CDS             complement(5103..5819)
                     /locus_tag="WGO65_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326377.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLGFEAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAV
                     GAVLALPVAALIAATNSSDK"
     gene            complement(5866..6378)
                     /locus_tag="WGO65_000006"
     CDS             complement(5866..6378)
                     /locus_tag="WGO65_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ0326378.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAIVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPNADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MESQGGTGEA"
     gene            6532..7863
                     /gene="pmbA"
                     /locus_tag="WGO65_000007"
     CDS             6532..7863
                     /gene="pmbA"
                     /locus_tag="WGO65_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ0326379.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8160..8363)
                     /locus_tag="WGO65_000008"
     CDS             complement(8160..8363)
                     /locus_tag="WGO65_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0326380.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8606..8908
                     /gene="clpS"
                     /locus_tag="WGO65_000009"
     CDS             8606..8908
                     /gene="clpS"
                     /locus_tag="WGO65_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ0326381.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8910..11201
                     /gene="clpA"
                     /locus_tag="WGO65_000010"
     CDS             8910..11201
                     /gene="clpA"
                     /locus_tag="WGO65_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ0326382.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLDRDLKNMVYGQENAIDALVTAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPGLGF
                     TAKREHGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKK
                     VEAEFTPALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTALA"
     gene            complement(11241..14339)
                     /locus_tag="WGO65_000011"
     CDS             complement(11241..14339)
                     /locus_tag="WGO65_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0326383.1"
                     /translation="MSLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSKPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSTDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKVATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTG
                     EVRRGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEALNETA
                     AKRYLISSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
     gene            complement(14336..15475)
                     /locus_tag="WGO65_000012"
     CDS             complement(14336..15475)
                     /locus_tag="WGO65_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0326384.1"
                     /translation="MGRSLTEIAEQLKGSNKKVQLIYAFNGTGKTRLSGEFKNLIAPT
                     SSEGADGEPTRRKFLYYNAFTEDLFFWDNDLLANEAPRLKIQKNSFTDWLLRDNGLDG
                     AVIKNFQYYTDDKLTPDFNDNFSEIVFYFARGNDEQIENIKISKGEESNFIWSIFYVL
                     IRQVIAELNIPEDSEEGRSTDQFDDLEYIFIDDPVSSLDENHLIQQAVDLADLIKSSE
                     SELKFIITTHNVLFYNVLYNELKLKNKNGSYLLLKNEDGSFDILEKRGDSNKSFSYHL
                     HLKGVIEKAIQNQQVERFHFILLRNLYEKTANFLGYEEWSNLLPEDGRQNYFQRIINF
                     TSHSTLSNEEFIEPTRQEQETIKLLLKHLLDNYNFFQSNEQRDNP"
     gene            complement(15518..16735)
                     /locus_tag="WGO65_000013"
     CDS             complement(15518..16735)
                     /locus_tag="WGO65_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0326385.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGEVAEIGTGSSNRQDEEHDG
                     EYPFYVRSKNILRSSTYQFNETAIIIPGEGGIGDIFHYAEGKYALHQRAYRVHVVIPD
                     LSPKFLYYFMNVDFKRYIQAKSVGATATSIRKPMLQRFPIPIPPLETQQKIVKILDKF
                     TELEATLEATLEAELALRKRQYQYYRDFLLDFDNQIGGGADGYQCRLKDVVWKTLGEV
                     TLPTENIKWKYTDKIYQYIDLTSVSREYKTITDTIAISANNAPSRAQKIVKEHDVIFA
                     TTRPTLQRFTIISKKFSGEIASTGYCVLRANCKEVLPKWIYYQISTERFNQYVEVNQS
                     GSAYPAISDAKVKEFKIPIPSLPEQEKIVAILDKFDTLTHSISEGLPYEITLRRKQYE
                     YYREQLLAFPKAA"
     gene            complement(16901..17911)
                     /locus_tag="WGO65_000014"
     CDS             complement(16901..17911)
                     /locus_tag="WGO65_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ0326386.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(17908..19452)
                     /locus_tag="WGO65_000015"
     CDS             complement(17908..19452)
                     /locus_tag="WGO65_000015"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ0326387.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTKPKLKDSKPFDAVVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(19525..20529)
                     /gene="rfaD"
                     /locus_tag="WGO65_000016"
     CDS             complement(19525..20529)
                     /gene="rfaD"
                     /locus_tag="WGO65_000016"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ0326388.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(20658..21689)
                     /locus_tag="WGO65_000017"
     CDS             complement(20658..21689)
                     /locus_tag="WGO65_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0326389.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKN"
     gene            complement(21691..22926)
                     /gene="dcm"
                     /locus_tag="WGO65_000018"
     CDS             complement(21691..22926)
                     /gene="dcm"
                     /locus_tag="WGO65_000018"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0326390.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFSGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(22963..23934)
                     /gene="hldA"
                     /locus_tag="WGO65_000019"
     CDS             complement(22963..23934)
                     /gene="hldA"
                     /locus_tag="WGO65_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ0326391.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(23989..24729)
                     /gene="pyrF"
                     /locus_tag="WGO65_000020"
     CDS             complement(23989..24729)
                     /gene="pyrF"
                     /locus_tag="WGO65_000020"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ0326392.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(25255..25902)
                     /gene="adk"
                     /locus_tag="WGO65_000021"
     CDS             complement(25255..25902)
                     /gene="adk"
                     /locus_tag="WGO65_000021"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ0326393.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            26117..26956
                     /gene="htpX"
                     /locus_tag="WGO65_000022"
     CDS             26117..26956
                     /gene="htpX"
                     /locus_tag="WGO65_000022"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ0326394.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(27259..27819)
                     /locus_tag="WGO65_000023"
     CDS             complement(27259..27819)
                     /locus_tag="WGO65_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326395.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(27933..28325)
                     /locus_tag="WGO65_000024"
     CDS             complement(27933..28325)
                     /locus_tag="WGO65_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0326396.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(28533..28925)
                     /locus_tag="WGO65_000025"
     CDS             complement(28533..28925)
                     /locus_tag="WGO65_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0326397.1"
                     /translation="MAKSFNQAASELTDIFPNISLTDFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(29042..29452)
                     /locus_tag="WGO65_000026"
     CDS             complement(29042..29452)
                     /locus_tag="WGO65_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0326398.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(29466..29903)
                     /locus_tag="WGO65_000027"
     CDS             complement(29466..29903)
                     /locus_tag="WGO65_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0326399.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(30315..31613)
                     /locus_tag="WGO65_000028"
     CDS             complement(30315..31613)
                     /locus_tag="WGO65_000028"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ0326400.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(31717..32868)
                     /locus_tag="WGO65_000029"
     CDS             complement(31717..32868)
                     /locus_tag="WGO65_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0326401.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            33203..34582
                     /gene="norM"
                     /locus_tag="WGO65_000030"
     CDS             33203..34582
                     /gene="norM"
                     /locus_tag="WGO65_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ0326402.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            34761..35801
                     /gene="murB"
                     /locus_tag="WGO65_000031"
     CDS             34761..35801
                     /gene="murB"
                     /locus_tag="WGO65_000031"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ0326403.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            35813..36463
                     /locus_tag="WGO65_000032"
     CDS             35813..36463
                     /locus_tag="WGO65_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ0326404.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            36518..37315
                     /locus_tag="WGO65_000033"
     CDS             36518..37315
                     /locus_tag="WGO65_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0326405.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHANKPVWFCLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDEIALPDPQTESARQILAAEQHLLDSGVPNI
                     DILRLGGLYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPSGRRLKN
                     IIEPRHPTRREFFTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            37366..37932
                     /locus_tag="WGO65_000034"
     CDS             37366..37932
                     /locus_tag="WGO65_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ0326406.1"
                     /translation="MSAFLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGRFSREPESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            37952..38842
                     /locus_tag="WGO65_000035"
     CDS             37952..38842
                     /locus_tag="WGO65_000035"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ0326407.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            38867..39625
                     /locus_tag="WGO65_000036"
     CDS             38867..39625
                     /locus_tag="WGO65_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0326408.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(39680..40345)
                     /locus_tag="WGO65_000037"
     CDS             complement(39680..40345)
                     /locus_tag="WGO65_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0326409.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(40545..41441)
                     /locus_tag="WGO65_000038"
     CDS             complement(40545..41441)
                     /locus_tag="WGO65_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ0326410.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            41510..42667
                     /locus_tag="WGO65_000039"
     CDS             41510..42667
                     /locus_tag="WGO65_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ0326411.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFY
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(42730..43731)
                     /gene="hemB"
                     /locus_tag="WGO65_000040"
     CDS             complement(42730..43731)
                     /gene="hemB"
                     /locus_tag="WGO65_000040"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ0326412.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEASNLGIPMLALFPVVTTNKTECAQEAYNPE
                     GLVPTAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGDKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(43914..44198)
                     /gene="yhbY"
                     /locus_tag="WGO65_000041"
     CDS             complement(43914..44198)
                     /gene="yhbY"
                     /locus_tag="WGO65_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ0326413.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            44308..44928
                     /locus_tag="WGO65_000042"
     CDS             44308..44928
                     /locus_tag="WGO65_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ0326414.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            44992..46959
                     /gene="ftsH"
                     /locus_tag="WGO65_000043"
     CDS             44992..46959
                     /gene="ftsH"
                     /locus_tag="WGO65_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ0326415.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            47180..47611
                     /locus_tag="WGO65_000044"
     CDS             47180..47611
                     /locus_tag="WGO65_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ0326416.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSVTLVEAFVKEADRRYA"
     gene            47604..47882
                     /locus_tag="WGO65_000045"
     CDS             47604..47882
                     /locus_tag="WGO65_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ0326417.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            47935..48513
                     /gene="pth"
                     /locus_tag="WGO65_000046"
     CDS             47935..48513
                     /gene="pth"
                     /locus_tag="WGO65_000046"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ0326418.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            48574..48780
                     /locus_tag="WGO65_000047"
     CDS             48574..48780
                     /locus_tag="WGO65_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ0326419.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            48777..49139
                     /locus_tag="WGO65_000048"
     CDS             48777..49139
                     /locus_tag="WGO65_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ0326420.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            49370..50785
                     /locus_tag="WGO65_000049"
     CDS             49370..50785
                     /locus_tag="WGO65_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ0326421.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVALLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            50904..51413
                     /locus_tag="WGO65_000050"
     CDS             50904..51413
                     /locus_tag="WGO65_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0326422.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            51580..52962
                     /locus_tag="WGO65_000051"
     CDS             51580..52962
                     /locus_tag="WGO65_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ0326423.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPK
                     NLQDLIVELKNGEAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(52982..53737)
                     /locus_tag="WGO65_000052"
     CDS             complement(52982..53737)
                     /locus_tag="WGO65_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0326424.1"
                     /translation="MIKIRNIHKTFGKNTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(53747..54463)
                     /locus_tag="WGO65_000053"
     CDS             complement(53747..54463)
                     /locus_tag="WGO65_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0326425.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(54453..55280)
                     /locus_tag="WGO65_000054"
     CDS             complement(54453..55280)
                     /locus_tag="WGO65_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0326426.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            55565..56473
                     /locus_tag="WGO65_000055"
     CDS             55565..56473
                     /locus_tag="WGO65_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ0326427.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT        13558 a        15393 c        13760 g        13806 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatga gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt cgaccgtgtc
      661 accaagctga ttgaaaaagg tctgattgag attgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tatccgtttc tttatccgtc caaccgatac atcaatcgaa ttttctgcat
     1141 cggattgcct catcttcgca aaacattgcc gaagtttcgc ggttgcacgc ctgacgcact
     1201 ttctgccgcg ccacgccttc attccgatcg cttgcctcaa acgtcttctg aaaaggcgta
     1261 acgctgcata catacatggt ggaatttgaa ccgaaaaggt cgattttgac ggtattgctg
     1321 ccgctttcca atacggcgac acggtgttcc aactgcgcca gacgtgcttc caaatcgccg
     1381 attctgtcgg caaatgcagg catggcggca cacgcggtca aaatgcccaa aatacatttt
     1441 ttcatcaatt tttccttgtt tcttgaaccc tacccacccg gacatccttt ttccgagagg
     1501 cagggtcaga ctttatttac ggggatgaat ccccttacaa atcaggataa tacatagaac
     1561 ggcactttat gcttcatccc atgtgttgaa acttcattgc tccttgtctg ttgaccctta
     1621 ttcccaatcc gttccctcaa ccttcggctt gggtttgcgc ctgcgacggc ggcgtttttt
     1681 cgcctgtcct tcgttttttt cgtgtcggtc ggcggcttcg tttttggtca tctccgaccg
     1741 ctgttccgtc gatgccgtct gaaacgccgt ccaccactcg gcaagggcgc ggtccgcatt
     1801 gccggtttcg gcgcgcaaga gcaggaaatc ataggcggca cggaaacgcg cctgtgcaaa
     1861 cagtttgtgc ggccttgcgc ctttgcggtt ttcaaactgc ggctggaaca tccaaatttc
     1921 gcgcatcgtg gcggaaaagc gttgcggtac gccccaaccg cgttcgacgg tttcgcgcat
     1981 cgtattgatt gcatcggaca gggcgggcac gggtttcaaa ccctgttgca gattgctttt
     2041 ccaatggcgt tccaactccg gccacatcag ggccgccagt acgaaaccga ccgaaaccga
     2101 tttgtcggca cgcagccgct catcggtatt tttcagggca agcaccgtca tttttccggc
     2161 gatgccgtct gaaacgcgca aagcattgag cagcggatgg atgtcgtccg gtatgtcaaa
     2221 tccgttcaaa cgtttcagac actcgcgagc gtgccctgaa aacagcaatt tcataatttc
     2281 gtcgaacagc ctcgctaccg gttcgtgctt cagacggcat atcgattcgg caatcggtgc
     2341 ggcggtttct tccgacagat caaagcccaa tttgcccgac aggcggatgg cgcgcaaaat
     2401 cctgacaggg tcttcctgat agcgttcggc ggcatcgcca atcataacca gcctgtgggc
     2461 ggcaacatcg gcaatcccgt tgtggaaatc caaaatctct tctttttcag gatcgtaata
     2521 caaggcattg caggtaaaat cgcgccgcat cgcatcttct tcgatgctgc cgtaggtgtt
     2581 gtccttcatg attctgccgt gcgcgttctg atgaaccttt gcaccgccgc gaaacgtcgt
     2641 tacttcgata atctccgcac cgttcatcac atggacaatc tgaaaacgcc tgccgatgat
     2701 gcggctgcgg cggaagagtt tgtgcacctg ttcgggcgtg gcatcggttg ccacatcgaa
     2761 atccttgggt tcgatgccga gcagcaggtc cctgaccgcg ccgccgacca cataagcctg
     2821 aaaccccgcc cctttcaggc ggcgtatgac gttttcggcg gcaaagctca acatttcggc
     2881 acggatgttg tgtctttcgg caggaatgac cgttttactt tccgcttttt tactgctccg
     2941 accggaaggc agcatcttat tcaaccattt tttcagcata aatattctga cccgaagagc
     3001 ggcatctcca cgggcaaccg tgttcagacg gcatcgggcg caggtttgca agatgccgta
     3061 accataaaca aaaggcgtag aaactacgcc gtccgaatca cgccgccctc gcggcggcaa
     3121 cgcgtcatta taacagattg ccctccaaaa aaatcagacc ctgcttttgt ggcggagtct
     3181 gaaatttgat gcaccttata tattgcgtcc ctaagaaagg atgattaaca aaaattaacg
     3241 ccctttactt tcttttccga ttgggaacat tcccgtcgta caaacaggca aatgaaggaa
     3301 cagaaatcac attcaaacgg cgttccgccg cctctacgcc gcttcccagt agtcgatata
     3361 aagctgcaat tcgaggttgt tgcgccattc gttggcaacg gggcggtaca ccgtgcggat
     3421 gtattcgggg atgtcttcgc tgcaacgcca aaacatcgct tcaaattcgc agccgtcttt
     3481 ttgcagccag acttttttgt gtttgccctc cgcgcccaaa ggttgctggc ggacgacgtg
     3541 gaactcgtcg gtaaaactcg gcggcgcgaa gccctgcccc caaacgtgtt tagcaaggtt
     3601 ttgcgcctgt tccagcgtaa tgtcgcgtgc aggcaggctg ccgtcggtga tgaaggtttg
     3661 cgacaaatcg tcttcgcaca ccatttcgcg cacggcttct tcaaaggccg tctgaaacgc
     3721 gggaatgttg tgttcgagta tgctcaagcc cgccgccatc gcgtgtccgc cgaatttcaa
     3781 aatcaaatcg ggatggcgtt tggacaccaa gtccaaagca tcgcgcaagt gaagattggg
     3841 aatggaacgc cccgaaccgc gcacttcacc gttgtcggca ggcgcaaaca cgatggtcgg
     3901 acgataaaaa cggtctttga ggcggctggc aacgatgccg accacgcctt gatggaagtc
     3961 gtcgcgatac gccaccaaag tcatctgacc tgaaggcagg gtttcaggaa aagcattcag
     4021 cgcgtcttgc agcatagact gctcgatttc gcgacgttcg atattgaggt tgtttaactg
     4081 cgccgccagt tcctgcgcct cggtatcatc tcgcgccaac aggcaggcaa taccgaccga
     4141 catatcgtcc agccgtccgg cggcgttgat gcgcgggccc aacgcaaaac ccatatcaaa
     4201 cggctgcgcc ttgcgccaat cgcgccgcgc cacttcaaac aaggcgcgga taccggggcg
     4261 cattttgccg gagcgcatcc gtttcaaacc ttgcgacacg aggatgcggt tgttgtgatc
     4321 gagggagacg acgtcggcaa cggtaccgag tgcgaccaaa tccaaaaggt cgcccagatt
     4381 cggctctttt aggccgtctg aaaaataatt gcggcggcgc agttcggcac gcaaagccat
     4441 cagtacataa aaaatcacgc ccacgcccgc caagcttttg ctttggaaac cgcagccttt
     4501 ttggttcgga ttgacgatga tgcagtctgg cacggtctcg gcaggcagat ggtggtcggt
     4561 tacaatcaca tccaaaccca attcctgcgc acgcgccacg cctgcgatgc tggcaatgcc
     4621 gttatcaacc gtaatcagca aatccacgcc ttgcgcggcg gcgatttcgg ccagctcggg
     4681 cgttaaaccg tagccgtgtt caaagcggtt gggcacgagg aaatctactt ttgcccccat
     4741 cgccgccaaa ccgtccaaac cgacggcaca cgcggtcgcg ccgtcggcat cgtagtcggc
     4801 gacaatcaag attttttcct gacgctcaac cgcatccgcc aaacggcggg cggcggcttc
     4861 gcaattcgtc agcgtttgat aaggcaggag ggaagcgagt ttgtcgtcca attcggcagg
     4921 actttgcaca ccgcgcgagg cacaaagccg ggcgattaaa ggatcggcgc cggcggtgag
     4981 caaatgattg aaaacgtcgg tattgacgga tcgggtttgg attttgacgg acatagcgtg
     5041 gaactttaca gtgcggatgt gtttgaaatg ccgtctgaag gcttttcaga cggcattgcc
     5101 gttcatttgt ctgaggaatt cgtggctgca atcaacgccg cgacaggcaa ggccagcacc
     5161 gcgcccaccg catccagtat caacgtggtg ggcgtatatg caatattttc aaacaacttg
     5221 gatttgtcgg tatgtttttt cgtaaccgtg taataaatat cggcaggcac actttgctca
     5281 aaatgataat cggcgttcag tttttgcggt gtggcgtagt atttgccttt ggcggatacg
     5341 cagcgcgtgt aaatagtccg attgtcaagt tcgaccgctt caaacccgag ctgtttcagc
     5401 ttggcgatgt cggcaggctt gtcggtatcg tagcgcaggc aaaggccttc ggtactgaaa
     5461 ttctggctgc caggcgattc gagtttgacc ggcagggctt ggtggcgagc atagctcggg
     5521 gtatcctcaa ctatttggaa gggtttgtcc agccctgcct tcaaaatgcc cgtcagcttc
     5581 gccgaatctt cgggattgac gacgaaccag tatttcccgc ccatcatcac cagactgccc
     5641 ttttccaatt gggcattgtc tttggcaacc acaccgaagg cgcggatttg atccttgtca
     5701 acgtgtttat aggcggtcgt ttggctgaac gggtcgttca tgccccacat cattgccgta
     5761 cagccgttca acatcaaggc tgccgccaaa acggcggcgg tcgttttacg gaacaacatt
     5821 ttatcttcca atcagaatga cggcggatgc cgtctgaaaa tatgcctagg cttcccctgt
     5881 tccgccttgg ctttccataa cggttttcaa ctcttgaaac agggcgcgga aatttttcgg
     5941 cggtttgttc tgttcctgtt cttttttggt attgcggatg agcgtcctca gcttgcccgc
     6001 atcggcgttt ggaaaatcgg acataaactg cgtcagcgcg ccgtcgtccg ccagcagctc
     6061 catccgcgtc cgctccacac gttgcaaaaa ggcgttgtgc gccgcatcgt cgccgcgcag
     6121 cttggcaaga aatgcctcga tgggcgcggg atcggtatcg cgcatcagtc tgccgataaa
     6181 ttgcgcctgc cgcttgagcg cgccgttgga tgtgattttt ttataggtaa cgatggcttc
     6241 gtacaaatct tcgtccaagc cgattttttt caacgtgtcg cttgagagct tggtcagctc
     6301 catacccaaa tcctgcaaac cgttcatctg ctttttcatt tgggttttgc tgacccattc
     6361 gtcttcttgt tcaaacatct tcattctcaa attttatccg acgggcgcat tttaacagaa
     6421 tgattccgcg ttcagacggc attttgcacc gtccgccctg caaacggacg cttttgcccc
     6481 tgccgcccga aacggttaaa atgccgcaac gctccaccaa gaaagatttc tatgctgttc
     6541 aaccacaccg cttccgaact tctcgacctc tgccgccgca cgctcgactt ggcaaaagca
     6601 gcgggcgcga ccgccgccga agccgatttc agcgaatcca tcggacaaag cgtcagcgtg
     6661 cggctgggcg aaatcgaaca aatcgagttc cagcaggaca agtcgctgga cattaccgtt
     6721 tacgtcggca aacgcaaagg ccgcgctggc accgccgatt tctccgaaca agccctgcgc
     6781 gacaccgtcc gcgccgccgc cgacatcgcc cgccacaccg ccgaagacgg ttgcgccgga
     6841 cttgccgatg cctgtctgat ggcaaaacac atcggcgacc ccgaccttta ccacgaatgg
     6901 gatttggata cggaagccgc cgtcggcttg gcaaaacaat gcgaacaagc cgccctgaac
     6961 gaggatgagc gcatcgaaaa ctccgaaggc gcggcggtgc aaaccggcca ttaccaatac
     7021 gtttacggca acacccacgg ttttgccgca caccggcaag gcacgcacca cagcatttcc
     7081 tgcagcgtcg ttgccgccga cgaaaacggc atgcagcgcg actactggta cgattccgcc
     7141 tgccgccatc cggatatgga cagccccgaa accatcggtc aaaccgccgc ccgccgcact
     7201 ttgcgccgac tcggcagccg cagcatcccg acaggcagct accccgtcct cttcgatacc
     7261 accgtttcgg gcggtcttat cggacacctc gtcggcgcac tctccggcgg cgcgctctac
     7321 cgccaaagca gtttcctgat cgacagcatc ggcaaaaaag tcctgcccga tttcctcaac
     7381 ctgcgcgaag aaccgcacat cccccgctct ttccgcagca gctattttga tgcggaaggc
     7441 gttgccaccg cgccgcgctt cgtgattcaa aacggcattg tcgaaggcta tttcctcagc
     7501 agttacagcg cgcgcaaact cggtatgcag accacgggca acgccggcgg cgcgcacaac
     7561 ctgtatttga accatacgca cgaaacgcaa tccgacctgc tgaaagaaat ggggacggga
     7621 ttgttggtaa ccgaactgat gggacagggc gcgaacacca ttaccggtga ctactcgcgc
     7681 ggcgcggcgg gtttttgggt ggaaaacggc gtgattgcct accccgtcca cgagattacc
     7741 gtagccggac gcttgcagga tatgtaccgc gacattgtcg gcgtggcgga tgacgctttg
     7801 cggcgttcgt ccaacaaaat cggctcgatt ctgattgcgg ggatgacggt tgccggaagc
     7861 tgaacgccct gccgatgccg tctgaagacg aaggcagcac gttttgcccc gtgcctgccc
     7921 gtttgccgtt ttatgccgca ccgccttttg ccgacaacat tgcacatttt ttcatcagca
     7981 ctgattttcc cttatccgtc aaaatactaa gccacttgtc cacaacaggg tacacacaag
     8041 ttcaggataa ggcgtgcggg gattggggtt tttccgccgc cggcaaaacg gcagacaaaa
     8101 acggcgggac attgcctgtc ccgccgtttt caaatataca tttgaccgta catcaggaat
     8161 taagcagcct gaatgttggc ggcctgtttg cctttagggc cggtggttac gtcgaaagag
     8221 acacgttggc cttctttcag ggttttgaaa ccttccatat tgattgctga gaagtgagcg
     8281 aacaaatctt cgccgccttc atcaggcgtg atgaaaccaa aacctttagc gtcgttaaac
     8341 cattttacga taccggttgc cattagaaac ttcctatact caaaaattaa caaaatcagc
     8401 aaaacaaggc atacggcaaa ccgagccgtc tggcgttcct tccagcttga tatactcggc
     8461 ggatgcccaa tcctgcttaa ccgtcttttt acatttgtta agacaaaacg tcaagctatg
     8521 ttttcaaaaa agtgggaaaa taggcaaaag taccgaaaat acaacaaaaa tgccgtctga
     8581 acgcggcttt caaatgggac acactatgac cgcgcaacac caatccgaca cgctcttgca
     8641 ccgtctgaac accctgccgc cgaaacgtta cggcgttttc ctattgaacg acgattacac
     8701 cacgatggaa tttgtcgttg aaatcctgac cgaaatcttc atgctcggac aagaacaggc
     8761 ggtagcggta atgctcttgg ttcatcacga aggcaaaggc ctgtgcggca cttacacgcg
     8821 cgatattgcc caaaccaaac agcaacaagt catgcagcgg gcaaaagccg aagggcatcc
     8881 gctgcaatgt attgtcgagg agatttaata tgcttgcacc cgaattggaa cagattttgc
     8941 agcagcttta ccgcgaggcg cgtaaggctc attatgaatt tatcagcctc gagcatctgc
     9001 ttttggtact catcgaagaa gatgcctccg tgccgaacgt cttaaaactc tgcggcgcgg
     9061 atttgaaagt ggtgtccgaa cagctcgccg ccagcgttgc cgaaaacacc cccctgattc
     9121 ccgaacacct tttagacacg gtcgaaaccc ggcccacgct cggcttccaa cgcgtgatgc
     9181 aacgggcgat ggtgcatacc cagtctgccg gaaaagccgc agtcgaaccg ttggacgttt
     9241 tggtcgcgct gatgagcgaa accgacagcc acaccgtcta tttcctcaag ctgcaatcgg
     9301 ttacgcgttt tgaagttttg cgctgtattg cccacggctc tcccgatgaa gatgaagacg
     9361 atggcaacta ttcttcagac ggcatggacg acgataatga aaaccgcacc aaaccgggca
     9421 aaaacccttt atcggcgtac accgtcaacc tcaacgccga agtcaaagcc ggccgtatcg
     9481 accctttgat tggtcgcaaa cacgaaatgg aacggctggt gcaaatccta tgccgccgcc
     9541 gcaaaaacaa tccgcttttg gtcggcgaag cgggcgtggg caaaaccgcg ctggcggaag
     9601 ggctggcaca tcaaatcgtc aacggcggca ttccagacgc gctcaaagat gccgaagtgt
     9661 acgcgctgga tatgggctcg ctgttggcgg gcacgaaata ccgcggcgac tttgaagcgc
     9721 gggtcaaatc cgtcttgaaa cagctcgaaa aaatcccgca cgccattttg tttatcgacg
     9781 aaatccacac catcatcggc gcaggcagca ccagcggcgg caccatggac gcgtccaacc
     9841 tgctcaaacc cgcgctggca aaaggttcgc tgcgctgcat cggcgcgacc acttacgacg
     9901 aataccgcac cattttcgac aaagaccacg ccttaagccg ccgcttccaa aaaatcgacg
     9961 tggtcgaacc caccgtttcc gaaaccgtac aaatcctgcg cggcttgaag ccgatgtttg
    10021 aagccttcca ccaagtccgc tatacgcaag gcgcactcga agccgccgcc gaactctccg
    10081 cacgctacat caacgagcgt ttcctgcccg acaaagccat cgacgtgatg gacgaagcag
    10141 gcgcggcgca acggattctg cccaaatcca aacagaaaaa agtcatcggc aaagcacaaa
    10201 ttgaaaccgt catcgccaaa gtcgcgcgga ttcccgaaaa aaccgtgtcg cacgacgaca
    10261 aacaagtgtt gcaattcctc gaccgcgatt tgaaaaacat ggtttacggt caggaaaacg
    10321 ccatcgacgc gttggttacc gccgtcaaaa tgtcgcgctc cggccttgcc ctgcccgaca
    10381 aaccgatagg cagtttcctc ttctccggtc cgaccggcgt gggcaaaacc gaagccgcca
    10441 aacagcttgc ctactcgatg ggcgtaccgc tgcaacgctt tgatatgtcc gaatacatgg
    10501 aacgccatgc cgtatcacga ctcatcggcg cgcctccggg ctacgtcggc tttgaacaag
    10561 gcggcctttt gaccgaagcc gtcaacaaac agccgcattg cgtgttgctc ttggacgaaa
    10621 tcgaaaaagc ccaccccgac attttcaacg tcctcctgca agtcatggac gcaggcaaac
    10681 tgaccgacaa caacggcaag agtgccgatt tccgcaacgt catcctaatc atgaccacca
    10741 acgcgggcgc ggaaagcctc agccgccctg gcctcggctt taccgccaag cgcgaacacg
    10801 gcgacgaaat gcaggcaatc aacaagctct tcacgcccga gttccgcaac cgcttggacg
    10861 cgattatccc atttgcaccc ttgtccgaac ccatcatcgt caaagtcgtg gacaaattcc
    10921 tgctccagct cgaacaccag cttctcgaca aaaaagtcga agccgaattc acgcccgcat
    10981 tgcacaaata tctggcggaa aaaggtttcg acccgcaaat gggcgcgcgc ccgatgcacc
    11041 gtctgattca ggaaaaaatc cgcaaaccgc tcgccgacga actcctgttc ggcaaactat
    11101 ccgacggcgg cttcgtgcgg atagactggg atgcggcaaa agaagaagcc gtgttgaagt
    11161 ttaagaaaag caaggtcaaa ataaaaaccg ccctcgcatg aaatcaaaaa tgccgtctga
    11221 aagattcaga cggcattttt ttaaatatct gtccctattc cggcaaactt ctccacaaac
    11281 gccgcgattt tttggaagac actttgtttc ttcgtcagat attgcggatt gagcggactc
    11341 attttcggca gggtttcggt cagttccgtg ccgttttcgc tggcatagcc gcgtttgagc
    11401 gagctgatta aatagcgttt agcggcggtt tcgttcaggg cttcggcggc aatcaatcct
    11461 gccgcttcgt gccgcatcac ctcttgcgcg taggtgtaaa aggtttccag tatggcggga
    11521 acgtcgggca ctttgtccaa atccgtatcg ttgatgaaat ccacaatcag accctctttg
    11581 gcgcggtgtc cgatacttgc gcggatgatg cggcggattt cttccaccag ctccgctttg
    11641 cctttgattt ttttgtggtg ttcgaaaacc agttgcaaaa tgtaatccaa attgatttcc
    11701 tgcgatttga gcaaatccac ctcaaaaacc acatcgtccc aatcgatttt cgattgctct
    11761 ttctgttcgc ctgctttttg gcggcgcaac caatcgcgga tgtcgttgta ggcggaacgg
    11821 tagtcctgca ccgccctttc agacggcatc ggcacttgcc gcatttcctg cacgtcttca
    11881 tcgctcaagt agtatttttc ttgaaacgcc ttcatcgcat cttcgtccgc cgcgtccaca
    11941 ctctgcaact cgcgcagcgc ggcaaattca tcgtagttct gcaatacgtt ttccgcccgc
    12001 aggtattcgc cgaagagttt ggcaaaatct tttttgtctt tttccgtttc gattttgtcg
    12061 ggatcgggga aacgctcgcg caattctttt gccacatcca aataaccgcg ccgcacttcg
    12121 cccgtccgat catcgatata gccgttcatg tattcttcgt agcttttttc caacaccacg
    12181 tttttggtgt ttttatcgcc gaacagggta atcgcatcaa tggttgcctg ctccaaatcg
    12241 cggaagcaga caatattgcc gaaggttttg gtggcatcgt aaatgcggtt ggtgcgcgaa
    12301 aacgcctgca tcaggccgtg atagcgcagg tttttatcga caaacagcgt gttcagcgtc
    12361 ggcgcgtcaa aacccgtcaa aaacataccg accacaatca gcaaatccac ttcctggttt
    12421 ttcacccgtt ttgccaaatc gcggtagtag ttctgaaagg ctttgctgtc cgtgccgaaa
    12481 ttggttttga aacaggcgtt gtaatcgttg atggcagcct gcaaaaattc tttcgcgctg
    12541 ctgtccatcg cttccggttc aaaggtctca tcaacaattt caccgacggc gttctgctct
    12601 tcgttggccg caaaggaaaa aatggtggcc actttcagcg ggtgcaggct gcctgcctgt
    12661 tgtgttttga acgcttcgta atagcacttc gccgcatcca cgctgctgac ggcaaacatc
    12721 gcgttaaagc ctttgccacc cgcattcagc cggtgcgttt tctgcctgaa ctggttcagg
    12781 atatattgcg tgatttcgcg gatgcgttcg gggtgcagca gggcttggcg gttttcggca
    12841 gcactcagtt tcttctcatc ctgttccgct tccacggctt tgaactgcgg gcgcacatcg
    12901 ttgtaatcca ctttgaattt caatactttt tcatcgcgga tggcatcggt aatcacataa
    12961 gaatgcaact cacgcccgaa cacgcccgcc gtggtttccg cgcccaaagc gttttcggga
    13021 aaaatcggcg taccggtaaa gccgaactgg cagaattttt taaatttctt tttcagattt
    13081 ttttgcgctt cgccgaattg cgagcggtgg cattcgtcga aaataaagac aacctgctgg
    13141 tgataaaccg gcagattatc ttcactcttc atcaggttgt tcagcttttg gatggtggta
    13201 acgatgattt tgttgtcgtc tttttccaaa ttgcgtttca aacccgccgt gctttccgaa
    13261 ccgttcacgc tgtcggtcga aaaacgttgg tattccttca tcgtctgata gtccaaatcc
    13321 ttcctgtcca ccacgaagaa aaccttgtcg ataaatgccg attccgtcgc cagacgcgcc
    13381 gccttaaagc tggtcagcgt tttgccgctg cccgtggtgt gccaaacata gccgccgctt
    13441 tccggcttgc tccaattctt cgcctgcgcc gaactgttga ttttccacaa aatgcgttcg
    13501 gcggcggcaa tctggtacgg ccgcataatc agcagcgtat cattcgcatc gaacacgctg
    13561 taatgcagca aaacgctcag taatacgctt ttttgcagga acgtggcggt aaagtctttc
    13621 aaatccttaa tcggatgatt gtccgaccgc gcccaattca tcgtgaaatc gaagctgttt
    13681 ttgtcgcgct tggtggtgtt ggcgaaatag cgcgtgtccg tgccgttgga aatcacgaag
    13741 atttgcagga atttgaacag cgaattttcg ctgttgaagc tctctttgct gtaacggtgc
    13801 acctgattga atgcctcgcg caccgccaca ccgcgctttt tcaattcaat ctgcaccagc
    13861 ggcaggccgt ttaccaacac ggtaacgtca tagcggtttg catgcgtgcc cgtctgctca
    13921 aactggttga taagctgcac atggttgcgg gcaaggtttt tcttgtccag cagataaatg
    13981 tttttcagac ggccgtcatc aaaagcgaaa tcgtaaatat ggtcgtcgtg gattttgcgg
    14041 gttttatcgg taatattttc agacggtctg tccagatatt ccgtcaaaaa ccgcgcccat
    14101 tcgccgtctg aaaacgccac atcgttcagc cgctgcaact gcgcgcgcag gttttccagc
    14161 agcctgcttt ggctgttcaa atccttgcgg tattcgtaac cctgattctg caaatcggcg
    14221 attaactccg cttccaaccg gttttccgat tggtagctgc ccgactgttc gattttttca
    14281 tattggtcga gcacgatgaa attcggcgtt tcggcgatgg gtttggtttc gaggctcatg
    14341 gattatctct ttgttcatta ctttgaaaaa aattatagtt atccagcaag tgtttcagaa
    14401 gtaatttgat agtttcttgt tcttgccgtg ttggttcaat aaattcctca tttgataatg
    14461 tagaatgact tgtgaagtta ataatacgtt gaaaatagtt ttgtctacca tcttcaggca
    14521 gaagattaga ccattcttca taacctaaaa aattagctgt tttctcatac aggtttctta
    14581 gtaaaataaa atgaaatctc tctacttgtt gattttgtat agctttttca ataactcctt
    14641 ttaagtgaag gtgatatgaa aaacttttat tggaatcccc acgtttttca agaatatcaa
    14701 aactaccatc ttcatttttt aacaaaagat aacttccatt tttatttttc aactttagtt
    14761 cattgtatag aacgttgtaa aataaaacat tatgtgtggt aataataaac tttaactctg
    14821 attcgctact ttttatcaaa tcagccaaat caaccgcttg ctgaatcaga tgattttcat
    14881 ccaaagagct gacagggtca tcaataaaaa tatattccaa atcgtcaaac tgatctgtgg
    14941 aacgtccttc ttcgctatct tctggaatat tcaattcagc gatgacttgt ctgattaata
    15001 catagaaaat gctccaaata aaattacttt cttcaccttt ggaaattttg atattttcaa
    15061 tctgctcatc attaccacga gcaaaataaa atacaatttc tgaaaaatta tcattaaaat
    15121 caggagtcaa cttatcatct gtataatatt gaaagttttt aataacagct ccatccagtc
    15181 cattatccct aagcaaccag tcggtaaaac tattcttttg aatttttaat cttggagctt
    15241 cgttcgctaa caaatcattg tcccaaaaga ataaatcctc agtaaatgca ttatagtaga
    15301 gaaattttct tcttgttggc tctccgtctg ccccttctga actggttgga gcaattaaat
    15361 tcttaaactc tccggataaa cgcgttttcc ctgttccgtt aaaggcatag ataagctgaa
    15421 cctttttatt gcttcctttc aattgttcag caatctcagt taaacttctt cccattatat
    15481 ttccttatca atattttcag atgacattat cccaatttca ggctgccttg gggaaggcaa
    15541 gcagctgttc acggtaatat tcgtattgtt ttcggcgcag ggtgatttcg tacggcaggc
    15601 cttcgctgat ggagtgggtc agggtgtcga atttgtcgag gatggcgacg attttttcct
    15661 gttcggggag tgaggggatg gggattttaa attctttaac ctttgcatct gatattgctg
    15721 gataagctga gccactttga tttacttcaa catattgatt aaacctttct gttgagattt
    15781 gataataaat ccactttggc aatacctctt tgcaatttgc tcttaataca caatatcccg
    15841 tgcttgcaat ttcaccagaa aatttcttac taattattgt aaaacgttgt agcgttggcc
    15901 gagtagttgc aaaaatcaca tcatgttctt ttacaatttt ttgagctcta cttggcgcat
    15961 tatttgctga tattgcaata gtatcagtaa tggttttata ttcccgacta actgaagtca
    16021 aatcaatata ttggtaaatt ttatctgtat atttccattt aatattttca gtaggtaagg
    16081 ttacctcccc caacgtcttc caaaccacat ctttcagacg gcattgatag ccatcagctc
    16141 cccccccgat ttgattgtca aaatctaaaa gaaagtcacg gtaatactgg tattggcgtt
    16201 tgcgcagggc taattctgct tccagcgtag cttccagcgt agcttccagc tcagtgaatt
    16261 tgtcaagtat ttttacaatt ttttgttggg tttctagggg tgggatgggg attggaaatc
    16321 tttgcaacat tggttttcga atagatgtag ctgttgcccc cacactcttt gcttgaatat
    16381 accttttaaa atctacattc atgaagtaat aaagaaattt aggggataaa tcaggtatta
    16441 caacatgaac tctatatgct ctttgatgta aggcgtattt accttccgca tagtggaata
    16501 tatctccaat tccaccttct ccgggaataa taatagcagt ctcattgaat tgatatgttg
    16561 atgatctaag aatattttta gatcgaacat aaaatggata ctctccatca tgctcttcat
    16621 cctgcctatt ggaacttcct gtaccaatct cagccacctc ccccaacggc ttccactcca
    16681 ctggtgccgt ctgaatcatc tcaatcaatt ttttcgcttt gctttgcata tccatggttg
    16741 ttttccgttt ctttccctct gcggcgggat gcggggcggt tgtggggtgc ttcggctcac
    16801 tctgtccgtt tgccgttatt tctgcgggct gaagtccgtc ctgctttcag gctgcctgca
    16861 atggtgtgca ggctgctttt tcaggtagcc tgaatggtag ttattttgtg cggtcttttt
    16921 tctgtttgat ctgttgcacg gctgtttttt ccaacgcttt aacgctttcc agataggctt
    16981 gctccaccgg cgacaggatg cgcgcttcat aggcgcggta ttcgcgttcg gctttctgct
    17041 ctgcctgttt gcggctgatg ctgcctgcac cctgtaatgt gccttgtccg tacagcccgg
    17101 aaaatttgtc caattcggct atccagtcgc gcatatacat ggggctttgc tcctgcgctt
    17161 tgatttccgc taggtcgaag aaggcggaaa ccagacggtt caggcggaac agttcgtctt
    17221 cggtcagata gtttttggcg attttggctt cattcagcgt ggggattgcg ccttgaaagg
    17281 tggtcagccc cataaagtct ttgctgctgt cggcacggct gtatatcagc tcagctgcgg
    17341 tttgccggct ggcggcatag tgcagtttgt tttgaacggc ggcaaaaaag gtttggcttt
    17401 cgctgctttt ggggttgtag tcttggctgg tggcatataa atcaagcact tgccggtata
    17461 gggctttttc gctgctgcgg atgtcgcgaa tgcggttgag cagttctttc caatagtcgc
    17521 cgccgcctgt gcctttcagg cgttcgtcgt ctatggcaaa gcctttggtc agatattcgt
    17581 ccagccgttc ggttgcccat tggcggaatt ggatgccccg cgcggaacgg acgcggtagc
    17641 cgacggcaat aatcatgggc agggaataat gggcgatttt gcggtttacc ttgcgcccgt
    17701 tttcattttg aactgtcaac tggaagttga cagttgcctt ctcttccaat tcttgctctg
    17761 caagaatggt tttaatgtgt ttgctgatat tttgtttggt ggtttggtac agttctgcca
    17821 tgtccgcctg cgtcagccaa agctgtccgc caaattcctg caaggcaaat tgggcagtgc
    17881 cgtcgttggc ggtgtatagg atgatgctca tgcttcaatc tctgcaatca cttcgtcaat
    17941 ttcacgccgc agccgttcga ttttggcaac ggtttcgctg atttcggcgt taagctgttt
    18001 gatgtcgata atttcgcggg tgtcttcggg ttcaacatag ctgctgacgg cgaggttgta
    18061 gccgttgtct ttgacggttt gctgggcggc gttttgggcg atatgcggca catcggcttt
    18121 gtcggcgaag agtttgacga tttcggcaat gtgttcttcg gttaagacgt tgttgttggt
    18181 ttctttttta aagaagccgc ctgcgtcgat aaattggatg tcggtattgt ctttgtgttt
    18241 ggacaaaacc aggatattga cggcgatgcc ggtgccgtaa aagagattgg gcgcaagggc
    18301 aatcacggtt tccacgtagt tgccctccac cagatattgg cggattttct gctctgcgcc
    18361 gccgcgataa aaaatgccgg ggaaagagac gatggcggcg cggcctctgc cggaaaggta
    18421 gttcagtgcg tgcaggatga aggcaaaatc ggctttggat ttcggggcga gtacgcctgc
    18481 gggggcaaag cggtcgtcgt tgattaaggt ggggtcgtcg ctgcctatcc agttaatgga
    18541 ataaggcgga ttggaaacga cggcatcaaa gggtttgctg tctttgagct tgggcttggt
    18601 cagcgtgtcg cccaattcga tgtggaattt gttgtaattg acgttgtgca gaaacatgtt
    18661 cattcgggcg aggttgtagg tggtgtggtt gatttcctgc ccgaagaagc cttcttcgat
    18721 gatgtgctcg tcaaactgtt ttttcgcctg taagagcagg ctgcccgagc cgcaggcggg
    18781 atcgtaaatt ttatttacct tttcctgccc gtgcaccgcc agccgcgcaa tcagcttgga
    18841 tacgctttgc ggggtgaaaa attcgccgcc ggatttgcct gcgttggcag cgtagttgga
    18901 aatcaggtat tcgtaggcat cgccgaaaag gtcgatgtgg tggtcttcaa aactgccgaa
    18961 atcgagttcc gccacgcctt ttaggacggc ggcaaggcgt ttgttcttgt cggcaacggt
    19021 gctgccgagc cggctgctgg tggtgtcgaa gtcgtcaaac aggcctttaa tgtcttgttc
    19081 ggacggatag ccggaggcgg agctttcaat cgcggtaaaa atttctttca gcttggtgtt
    19141 gagctcttcg ttttgatggg cttcggcggc aatattgcaa aaaagctggc cggggtagat
    19201 gaaatagcct ttgactttga cggcatcgtc tttgatttcg ggcgtgatga tgctgtccgg
    19261 catagcggcg taatcaatac tgctgtcgcc tgcctgcata tagtcggtaa agttttcgct
    19321 gataaagcgg tagaaaagtg tgccgagaac gtattgttta aagtcccagc cgtccaccgc
    19381 gccgcgtact tcgtcggcaa ttttccaaat ttggcggtgc agttgggcgc gttgttgcat
    19441 ttcggtcatc atcgaaatcc atataaaagt taaacaaatc gaaatcgcct gatattttca
    19501 gacgattttt tacgggcatt caaattaagc caaattttcc agcatccatt tgacgtagcg
    19561 ctcgacgcct gatttgacat cgaaaaattc ttccgtataa cctgcctcac gcaatttggt
    19621 gatgtcggct tgagtgaagc tttggtattt gcctttgagc gcatcgggaa atgggatgta
    19681 gcggataagt tcttcttcta ccagctcttt caagctcatt tcgggtttgc cttcggcggc
    19741 gcggcaggcg ttgacggtgg cggcggcgag ttcgttgaac tgttggctgc ggccggtgcc
    19801 gaggttgtag atgccggaaa gttcgggatg gtcgaagaag tagaggttga ctttggcaac
    19861 gtcttcgacg ctgacaaagt cgcgggtttg ttcgccgttg ccgtagccgt cgttgctgcc
    19921 gaacaggttg acgtaaccgt gttcgcggta ttggtggaaa tggtggaagg cgacggatgc
    19981 catacggcct ttgtgttgtt cgtgttgtcc gtacacgttg aagtagcgga agccgacgac
    20041 ttgggcggtc aagccttctt tcatgcggcg gcgcaatact tggtcaaaca ggaatttgga
    20101 atagccgtac acattgagcg gtttttcgag ttcgcgctct tcacggaaga tttcgccttt
    20161 accatacacg gcggcactgg aggcataaag gaagggaatg cgttcgtcct gacaccagtc
    20221 caacaaatcc agcgtgtact ggtagttgtt ttccatcata tacaaaccgt cgtgattcat
    20281 ggtgtcggaa cacgcgcctt gatggaaaac ggcttcgatg ttttgataag gtaaaatgtg
    20341 ttccctcact tggcggatga attcatgttt gtcgagataa tgggcgattt cgcactcggc
    20401 aaggtttttg aatttttcgc ctttgctcaa attatcgacg gcaacaatgt cagtaatacc
    20461 gcgttgatta agtgctttga cgatgttgct gccgataaag ccggccgcgc ctgttacgat
    20521 gatggtcata ttcggtttcc tttgttttga gtaaaattaa taaacttagt tacaagaaat
    20581 catcgatatt gtcctgaaac acaaatttgc gatgatattc caaatatttt tctcttcctt
    20641 cgggatatat tctcatctta attttttgca tttggattga tattaagttt ttcccatgtg
    20701 taacggctta gtcgagtacc gcaaatcaaa cgaccatcat ccaagaaagt aatataacct
    20761 tgatcgaaca accaatcata agtcggcgtt aaagctaacc catttaaata atctaatgcc
    20821 tctttctcat ttttctcagt aatacaaacc atataaggtt taatatgact agcccttaac
    20881 aaaatctcat ctgtaattaa tgtaaatgga cattgcggca tatgatttat aacatccttg
    20941 cgatatttct gctgcccttt tctacttttc tgtttaatga tttcttgctc tacgaattga
    21001 ttaaattctg aagccggtat ttctaatttt tctcttgata agagttgcgg gtgcacgata
    21061 gagcgaaatt gataatccaa aaatattcgg aaataaaata atggttctga atcttctata
    21121 tctttgacag gcaatagttt aagaattgat aaataactaa ttttgggcaa tacaatctta
    21181 cgccaaattg accatatatc atcctccgaa cggatataac ccctacggta acctttagaa
    21241 ttttccaaat ggtctgatac atcatggatt gaaaagaaaa tattttctgt aggcagatta
    21301 ttaatactta aaacgtaatc atcataaaac cttgaaatat catgatgata tttctgttct
    21361 tgtttgtgat attcaacttt tgcatcaacc aaataacgct gtaaattttt cttattaaaa
    21421 aaacaatttt tctgaatgaa aggatattct accctccttc cattttttgt ctcagtaatc
    21481 ccccaatttt catactcaaa aaatgtactt agttttcgac cactatcatc aatataacta
    21541 cctacatatt tgcgtgcttc cccattacca ccaaaaacag ataacttgtt attcctgtga
    21601 ataaaactat cttccgcacg taaattttga atactatcga caataaaata ctctatgttt
    21661 tcaatagtaa ttctttgaaa atccggcatt ttactctcca taaagcgtaa tatccatttg
    21721 ttttaatata gcgattaata catcaacaac aatactgttt cccgcttggc gatacatttg
    21781 agtatcacta actaaaattt taaaatcatc tctaaaaccc attagccgca aacattccct
    21841 aggtgttaat ttacgaatac gtccacgatt atgagtaaca taattatcaa ccccggcacg
    21901 atgcatttta tgcatagttt gcaataatgg gcgagctaca ggcaaatcag tttccgtact
    21961 ggttttaaaa ttttttgttc ctcctgccaa aacataattt ttgatttttt cagaaagata
    22021 atatttttcc tcaacgttat ttacatcaaa aataaagtca tcaaattcag attcgcgggc
    22081 tgcctgaaaa ataaaatcac catgccaatt aaattgttga tttgcttttt ggcataaggc
    22141 aatttctcca ttaatctgtg tataacgttt ttgtctattt ttagaactgg taacaaattt
    22201 cacacccttt tcacgtaaaa aatatttgct atcagtatag tcctccaaaa aatcttgcat
    22261 agtatgttct aattcaatct tttcaggaaa ctgaaaacca tttataggag gggtatgaaa
    22321 gccaacaaca aaaatacgct cacgatgttg aggaatccca taatccttac tattcattat
    22381 ttggaaatat aagtcataac caagtgaata aaaaacactt tttacaactt tccaagtttt
    22441 tccattatca tgattaagca agccctttac attttcataa ataaatatct ttggttggac
    22501 ttcatccaca acgcgagcaa attcatagaa taatgttcct cgtgtatctt ctaatcctgc
    22561 acgtttgcca accatggaaa atgcttggca aggactgcct ccaactaaaa tatcaacttg
    22621 atttctaaac tttctcgcat caaattgagt aatgtcgtta tgccaaaaat cttcatttaa
    22681 tttatagttt ccaagataac tttttttaac gtatggatca atatctcctg aaaaaacaat
    22741 ggtatggttt aaatttaatc ggtggaatgc ctgttcaacc gcaccaatcc cgctgaatac
    22801 ggttgctaat ctaatatgtg aatcaggttt aagaaaagtt ttagatttcc aaccttgttg
    22861 actgggaaag agcaaagttt tttgtaatcg agtatcgtgt gtctgtgcca ttgtcgaaat
    22921 agtcatactt atatcgttct gtttatctta tcaatatgaa aactacattg ttgattgccc
    22981 tgacaatgcc ttgatcaatt cggcaaacga gcaaaccgcc gtaccgagtt tcgccacgac
    23041 aaccccggcc gcagtattgg caaggtacat ggcttcgggc atggtgcagc ctgccgccaa
    23101 acccaagccc attccggcaa tgacggtgtc gcccgcaccg gatacgtcgt aaacttcttg
    23161 ggcgcgggtg ggctggtaaa tcggttcgcc ttcgctgaac aaggtcatgc cttcttcgct
    23221 tcgggtcagt aaaacggcgg tcaggtcgag gtggcggcgc aggttttgcg ctttttcggt
    23281 cagctcgctt tcgtttttcc aactgccgac cacttctttc aattcggcgc ggttaggcgt
    23341 aatcagagtt gcaccgacat atttttcgta atcgtcgcct ttggggtcga ttaagacggt
    23401 tttgccggcg tgtttcgccc aatcgatcat atcggagata tgcgacaggc cgcctttgcc
    23461 gtagtctgaa aaaatgattg cgtcgtattc gggcaagatt tcgcggtatt tctgcttgat
    23521 ttgttccaac acttcgcagt tgggatgttc ttcaaaatca agacggataa gctgctggtt
    23581 gcgggcgacg acgcgcagtt tgacggtggt ggcgatttgt ttgtcgcgca tcagatagga
    23641 ggcgacgccg tcctgcacca tcagcgcatc gagcgcgtcg gcggcttcgt cgttgccggt
    23701 tacggacaac agccctgccc tgccgcccaa cgaagcgatg ttgcgcgcga catttgccgc
    23761 tccgcccgcg cgttggtcga ttcgtccgat tttcgccacc ggcacggggg cttcgggcga
    23821 aatacgggac acatcgccga accaatagcg gtcgagcatc acgtcgccga caaccaggac
    23881 tttggcttgc gcgaaacggg atttgagggt ttcttgttgg aacttggcgg acatattgcc
    23941 tccgttgtat tttttcagac ggcctcagcc tgtaaggccg tctgaaaatc agtttgcttc
    24001 aaggtttgcc acgcggttca cttcgcgcaa tacggctacc ggatcggcag cttgggttac
    24061 aggacgaccc attaccaaat aagtcgaacc ggcagccaag gcttcggccg gtgtcatgat
    24121 gcggcgctgg tcatcattat tgccggcaac gtccaagcgg atgccgggcg tgaccaagac
    24181 aaaatcctgt cccaattcgc ggcgcagcgg cgcggcttct tgggcggaac agaccacgcc
    24241 gtccaagccc gaactttgcg ccagttttgc caagcggatg acttgttctt caggggcggt
    24301 gttcaaaccg atttccgcca aatcgctttg ttccatgctg gtcaacacgg ttacgccgat
    24361 taagagcggc ttcgtgccgt atccggcaac ggcttctgct gcggcttcca tcatacggcg
    24421 gccgcccgat gcgtgcatat cgaccatcca aacgcccata tcggcagcga ctttgcaggc
    24481 ttgcgcgacg gtgtggggaa tatcgtggta tttcaaatcg aggaaaagtt tgaaaccttg
    24541 attgattaag ctttctgcca aattgcgtcc cgtcgcggta aacagctctt tgccgatttt
    24601 gatttgacac aatgtcgggt caaggttgcg gacgaatccg agcgtgtctt tttcgttgga
    24661 aaaatcaagg gcgacgatga cgggggtgcg ttgttgcgga gtttggaagt cggagattaa
    24721 aggattcatg gtgttttcgt ttcgggtttt cagacgacct ttatcgtctt tttcgcagta
    24781 tagtggatta actttaaacc ggtacggcgt tgtctcgcct tagttcaaag agaacgcttc
    24841 tctaaggtgc tgaagcacaa agtgaatcgg ttccgtacta tctgtactgc ctgcggcttc
    24901 gtcgccttgt cctgattttt gttaatccac tataacaacg taaattcaaa ccgtaggtcg
    24961 ggcattgatg tccgacctac gcggactcaa gcccccgaca aactgtttcg gataaatttg
    25021 aaccggtaca tcagccgtca ttcccgcgca ggcgggaatc cattgataaa actcagttca
    25081 tcagatttaa acagcgattg cccaaattca gaaatggatt cccgcctgcg cgggaatgac
    25141 gccaaatgat tatttttcgg ttgcaacacg ctgtttatca aaaacaggtc gtctgaaaac
    25201 ctgattttct gttttcagag tgcgaagcct gcccgtgggt acgacctttt cgatttattt
    25261 gcccaatgcg cccaatactt cggctttcac ggcttctacc ggctgagtgc cgtcaacttt
    25321 gatgtattta ggcgcgtgtt cgccttccag tttgctgtaa aaatcaacca aaacttcggt
    25381 ttgctcgtgg taaacggcaa ggcgtttttt cacggtttct tctttgtcgt cgtcgcgctg
    25441 aatcaaatct tctccggtta cgtcgtcttt gccttcaact ttgggcgggt tgtaggtaac
    25501 gtggtaagta cggccggaag ccaaatgcac gcggcggccg ctcatgcggt cgacaatcac
    25561 gctgtcaggc acgtcgattt caacgactgc atccaaatcc acgcctgctt caaccatcgc
    25621 ttcggcctgt gccagcgtgc gcgggaaacc gtcgaacagg aaaccgtttt tgcagtcgtc
    25681 ttgcgcgatg cgttctttga ccatgccgat aatgatgtcg tcgcgcacca agccgccttc
    25741 gtcaatgatt tttttcgctt ccaaacccaa gggcgtgcca gccttaatcg cggcgcggag
    25801 catgtcgccg gtagagattt gcggaatgcc gaacgcggcg gtgatgaatt gcgcctgagt
    25861 gcctttgccc gcgcccggcg cgcctaaaag taatgctttc atgaagtgat cctttgaaat
    25921 gtgtttattg gaatcaaaca gatggtttac gattctacac ggcttggcac aatagttcaa
    25981 aaaccaactg cacaaactgc catttgcaag caaatgtttt gcataccttt gccgagtctg
    26041 atattcacaa gccggcaact ttttgggaca atccccaaaa tttcaaaaca tcacacccta
    26101 ccaaaaagga acatccgtga aacgaatttt tctgtttttg gctaccaata tcgctgtttt
    26161 ggtcgtaatc aacattgttt tggcggtttt gggcatcaac agccggggcg gcacgggcag
    26221 cctgttggcg tattccgccg tcgtcggctt cactggttcg attatttcgc tgctgatgtc
    26281 caaattcatc gccaaacaat cggtcggcgc ggaagttatc gacacgccgc gcaccgaaga
    26341 agaagcctgg cttttgaaca ctgtcgaagc ccaagcgcgg caatggaacc tgaaaacgcc
    26401 cgaagtcgcc atctaccact cccccgaacc caatgccttt gccacgggcg catcgagaaa
    26461 cagctccctg atcgccgtca gcaccggttt gctcgaccat atgacgcgtg acgaagtgga
    26521 agccgtattg gcgcacgaaa tggcacacgt cggcaacggc gatatggtta cgctgacgct
    26581 gattcaaggc gtggtcaata cctttgtcgt gttcctgtcg cgcattattg ccaacctgat
    26641 tgcccgaaac aacgacggca gccagtccca gggaacttat ttcctggtca gcatggtatt
    26701 ccaaatcctg ttcggcttcc ttgccagctt aattgtcatg tggttcagcc gacaacgcga
    26761 ataccgcgcc gacgcgggcg cggcaaaact ggtcggcgcg ccgaaaatga tttccgccct
    26821 gcaaaggctt aaaggcaacc cggtcgattt gcccgaagaa atgaacgcaa tgggcatcgc
    26881 cggagatacg cgcgactccc tgctcagcac ccacccttcg ctggacaacc gaatcgcccg
    26941 cctcaaatcg ctctgagccg atttgaaaca acaaaaaaac cgtacgcgca agcagtacgg
    27001 ttttttgtat atgctgtctg aaaacatcag gtttccatac cgacaccaac cgtatccatt
    27061 tccgcaccgg atacgccgca ttgatatacc ctgataaacc gtatccggac agaaaatata
    27121 gtggattaac aaaaaccagt acggcgttgc ctcgccttag ctcaaagaaa acgattctct
    27181 aaggtgctga agcaccaagt gaatcggttc cgtactattt atactgtctg cggcttcgtc
    27241 gccttgtcct gatttttgtt aatcccctat atcagataaa acagcggtaa aattgccgtc
    27301 tgaaaccaac tctgcgccat acgggacacg ctctgcacgt ctatttgcct ccgatatatc
    27361 ctccctactg taggaacgtc tgccgatttg cacgaaatcg gcacctctag cacccttcag
    27421 gatggcggac gttatccgtt cttgggattc tgcaagacgg tcgacggcat tatacagacg
    27481 ctcggtctga tacccttccg aagcaacctg cttcaggtat tccaaataac cgtaacccaa
    27541 cgcccctccg acaagcaggg tcgtcaacgc taccagcgtt gccgaacgga ttttaggggg
    27601 catatcttga agcatatctc taccgaaccg aaccaaagtc tctttaaagt cggactctac
    27661 acgcgtagaa ccctcggaaa tcttgaagtt caactcatac tgcttccgtt tgtccgctgt
    27721 caaccgcttg aggttgtcat ccccgtattg gacggttgac acaaccctgt acagactgtc
    27781 ctgtatttcg accagcgtct gtgcaaagtt ggcggtcata actggattcc caacccttgc
    27841 cgccgattct gatactcaaa acggtatcca ggcgttccag cagggcttcg ggagacagca
    27901 gtgcgtattc ttcaatattt tccataggtt gattatagca gaaccttata cctgttacag
    27961 acaaggttgt cgggatttga acgtccgtcg agcttggttc gtatagagcc gtagggggtg
    28021 tttagggctt tggcggcagc ttgtacgctg gggaacgtga gtgtttcgcc tgtggcggtg
    28081 tcgaggatgg agacaggttt ttggttttgg ggaggcgtat tttccttcat tttaagtttc
    28141 ccgcactccg gacacccata ctttgacttt atagctgtgt cgtaatacaa aagttggaca
    28201 accccgtgcg taggacatct aacactggaa ggtttataaa tgccactgaa ctccaacaat
    28261 tctaaatgtg gaaatttctc ttgaaagcgt gatttggcgg tatcgaaagt cattcgctta
    28321 cccataattt gtctccatag gttatgtgca aatggattac acaaccaaaa tatagaaatt
    28381 ttcaaatttt ttgagaccta cctgaaatcc tgcgcaagca caatgtaaag aaaaatggta
    28441 tacgtcttta ctaaatcttt acataaatta actctaaccg ggatttgaac gtccgtcgag
    28501 cttggttcgt aggatttagg gtgttgtgaa ccttacctat cgtacccaag cactttgtac
    28561 ctgtttgaaa taaggttgtc gggcgacgtt cgacccttga gcctgtgatt gatttgttgg
    28621 aaatgtacac ctaaagcagc acctgcggca gtaacgcttg ggaacgtgag tgtttcgttg
    28681 gtcgttgtgt cgaggatgag gaggggcttc cctacatttg gaggtgtttg ggtttttgac
    28741 atcttagcac actcaggaca cccgtacttt gactttataa gggcattaaa tgtcgaataa
    28801 cgaacgttcc cgtgcatagg acaattaacg gttactgggt aattcacacc gtcaaagtcg
    28861 gttagagaga tattagggaa tatatcagta agttcagaag ctgcttggtt gaaagatttg
    28921 gccatttgag actccaaaat tgattgtcaa atggatttaa tagcaaattt ataaaaatgt
    28981 caaatttttc gagaagcacc tgatagtggc gaggggtata cactgttaaa aagtgtatac
    29041 gtcatttagt gcagatgtga accttatacc tccctgcaag taaagtctct gtgtgaaccc
    29101 gaccttttat acggtcagtg atagctgctg ggtttatacc caatgacctt gcagcagatg
    29161 ctcttgaggg gaatgtaagt gtttcgccag ttacagtatc ctccaatact gtatttttag
    29221 ataatttatc tattgtatct tctggaattt tgtgggaatt gacaccataa gatgcacatt
    29281 ttggacaccc acttttagag tccaacatac ttttgaatgt agagcaagta accctcccgt
    29341 gtataggaca gacgattacc gtcgggtaac gaacgccatt gaactccaca agattcaaat
    29401 gtggaaattt agaggttagc ttagaaactg cttgagtaaa tgtaagtgcc atagtggttg
    29461 tgaacttact ttaccaacct ataacgatac ctaatcaatc ggtcaaacgg agtgcgtccg
    29521 cttaaacgta cagctaaagt gctgttggag caaccgataa atttactggc agccagtgta
    29581 cttgcaaact cgtagatttc ccctgtctct gtgtcctcca acctaatggc aacggcgttt
    29641 ggagagggct tttcgggatt tcgagtacat tcaggacatc ctgttgagga tttcatggca
    29701 gcttgtagat tcccgtaaac aaccgaccca tgcaaagggc attggattgt taccggttcg
    29761 tagtttttag tgaacttcaa aaccgtctta tcaggaaatt tactggaaaa ccttaactga
    29821 acatcttgta gcgagagtga ttttggcatt atagaccttt ctaaaatgaa agcattatac
    29881 aaccaaaata tagaaatttt caaattttct ctgaacgcac tggtatccga tggagctaaa
    29941 tgtaaagaaa aatggtatac gtctttacta aatctttaca cattttaact gtatagtgtt
    30001 tgacaaacaa caggcatagg cgcataatgc acccgtagtt agatagatag taaggagata
    30061 accaatgact tagcacctgc ccccctctct ctcgcgcacc ccgcccctcg cttgcggcta
    30121 atgggctttg cgcgacatat acactttcat ctcctgcatg gcttaactgc ctatgcaata
    30181 gtacacacat caacaaatca ggctggcacg tcttggcatt ggctgacgta ccattgtagc
    30241 ccgaaatgtt gataccctac tatttatgac caacggcgga ttagggcttc agacggcaat
    30301 tttactgcaa aaccttatgc gaacggatga tgcagcacaa tcgtttcttc gcggtcgggg
    30361 ccggtggaga cgatggcgac cggcgcaccg cagacttctt caatccgttt caaatatgct
    30421 ttggcattgg caggtaatgc gtcgtagctt ttcacgccga aagtggattc acgccagccg
    30481 ggcatggttt cgtaaatcgg cttgcaggtt tccaccgcat cggaaccgca aggcaggatg
    30541 tcggttttgc cgccgtcggg caattcatag ccgacgcaga tattgatggt ttcaacgccg
    30601 tccattacat cgagtttagt aatacacata ccggaaatgc cgttgatttg gatggagcgt
    30661 ttcaatgcgg cggcatcaaa ccagccgcag cggcgtgcgc gtccggttac cgaaccgaat
    30721 tcgtgtccgc gttctgccaa acctgcgccg acttcgtcga acaattcggt cgggaacggg
    30781 cccgaaccga cgcgcgtggt ataggctttg acgatgccca aaacataatc cagcatttga
    30841 ggacctacgc ccgcgcctgc ggaagcggct cccgccagac agttggacga ggtaacgaag
    30901 ggataagtgc cgtagtcgat gtccaacaac gcaccttgcg cgccttcaaa cagcagtttt
    30961 tcgccgtttt tgtttttctc gttcaacacg cgggacacgt cggtaatcat cggcgcaatg
    31021 cgcggcgcga ctttttcgat aaccgccatc acgtcttccg ctttaaccgg ctcggcattg
    31081 tgcagatgtt gcagttggac attgtaatag gcaaggacgg catccagttt ttcacgcagt
    31141 ttttcaggat gcagcaaatc ggcggcgcga atggcgcggc gtgccacttt gtcttcgtag
    31201 gcagggccga tgccgcggcc ggtcgtgccg attttgcctt tgccgcgcga tgcttcgcgg
    31261 gcttggtcga gcgcgatgtg gtaaggcagg atcagcgggc aggtcggcgc gattttcaga
    31321 cggccttcga cgtttttcac gcctgccgcg ttcaactcgt cgatttcgcc caacagggct
    31381 tcgggggaga cgacaacgcc cgaaccgatg aagcagtcca aactttcatg caggatgccg
    31441 ctcggaatca ggcgcaaaat ggtttttttg ccgccgacaa ccaaggtatg gcccgcattg
    31501 tggccgcctt ggaagcgcac cacgccgccg gcttcttccg ccagccagtc aacgatttta
    31561 cctttaccct cgtcgcccca ctgtgcgccg attactacaa catttttagc catagccata
    31621 taacctatcg atattaaaaa ttattgcgga aatccccaaa ccggtgcggc tttgcgcccc
    31681 gccacgattt gcctttcatc cgccatgaac gggtatttag cgtttcacga cctgccaaac
    31741 gccgtccgtc tttttcagac ggcctgcaag ctcttccgaa acattgtgtc cgataccgta
    31801 gtcaatcacg acacactgcc cttgttcacg caaggcttcg actgcttcgc gcgccgcttc
    31861 ggcatcttcc gcatcgacca acacggcggg ctgccgttcg atggtgggca aacgcccgat
    31921 aaagctgcgc aagtcgaaac tgaatcccgt tgccgggcgc gccctgccga aatatccgcc
    31981 caatccgtca taacgcccgc cgcgcgcgac cgcgtcgtgg aaatcggcgg cataggcggc
    32041 atacagcaag cccgtgtggt aattgtcgac acgcagctcg gacaagtcga tatggatttc
    32101 acaatcgggg aatgcgtcgc acaccgcctg caattcgccc aacgcgccgc cgaccgccga
    32161 caaatccggc aaccgtccgc gcgcgtcgga caacacttca cgcccgccgt acaggcgcgg
    32221 caacagcgag aatgcttttg cccacatgcc gtccagcttc caagccttga cctgcgcttc
    32281 gaccgccccg gtatctttat cctgcatcaa ggcaagcagc gttgcggact gccccgcatc
    32341 caaatgtgcc gcatcggaca aggcgcgaaa tatgccgata tgccccagcg aaagcagcac
    32401 tttgcccata tcggcaattt tcatgctttt cagcataagg tctatcagct cgatgtcgcc
    32461 acggatgtcg gcaaaaccgt acatttccgc ccctgcctgc aagggttcgc gcatattcag
    32521 cagaccgtcg ggctgcgcgt gcaacaccgg accggcataa cacaaccgat taatcccttg
    32581 gttggcggat aaaagatggg catcgatacg cgccacctgc ggcgtgatgt cggcgcgtat
    32641 gcccaactgc ctgccgctga gcctgtccgt taccaaaatg gttttcaggg aaagccccgc
    32701 atcgatatgc gtcagcaggg aatgtgcgta ctccatcagc ggaggctgta ccagttcata
    32761 accgtgtacg cggaacagtg ccaacaactg ctccctcgcg ctttcaagct gccgcgcgtt
    32821 cgtgggcagt acgtcggcga tatgttccgg aagctgccat gtctgcatga gaatctgcct
    32881 tctattttat catgtaaata caaaaggctg cccgatccgc aatcagacgg catctttgcc
    32941 gcaacgcatc acgccgctgt caaaacggcg atgcttcctt ccgattcaga atccgaacag
    33001 cccctgtccg acggctcaat gccgtcaagt cgttaaaacc aaactttacc ataaaataca
    33061 cacaatctga atatactttt ataccctgta tccgccgtct gacggcacgg cattttttta
    33121 ctatataatg tgcaccatta tttctcatca cttcctgctg ccgttttctg cttcagacgg
    33181 catttttgtt gattgaacac ttatgctgct cgacctcaac cgcttttcct tttccgtctt
    33241 cctgaaagaa gtccgcctgc tgaccgctct tgccctgccc atgctgttgg cgcaggtcgc
    33301 gcaggtgggc atcggtttta tcgatactgt gatggcgggt ggtgcaggca aggaagactt
    33361 ggcggcggtg gctttgggca gcagcgcgtt tgccacggtt tatattacct ttatgggcat
    33421 tatggcggcg ctgaacccga tgattgccca gctttacggc gcgggtaaaa ccgacgaagt
    33481 gggcgaaacg gggcggcagg ggatttggtt cgggctgttt ttgggcgtgt tcggcatggt
    33541 cttgatgtgg gcggcgatta cgccgttccg caactggctg accttgagcg attatgtgga
    33601 aggcacaatg gcgcagtata tgctgttcac cagcttggcg atgccggcgg caatggtaca
    33661 ccgcgcactg cacgcctacg cctccagcct gaaccgcccg cgcctgatta tgttggtcag
    33721 ctttgcggcg tttgtgttga acgtgccgct gaactatatt ttcgtttacg gcaaattcgg
    33781 tatgcccgct ttgggcggcg caggctgcgg actggcgacg atggcggtgt tttggttcag
    33841 cgcgctggca ttgtggattt atatcgccaa ggaaaatttc ttccgcccat tcggactgac
    33901 ggcgaaattc ggcaaaccgg attgggcggt gttcaaacag atttggaaaa tcggcgcacc
    33961 catcgggctg tcttattttt tggaagccag cgcgttttcg tttatcgtgt ttttgattgc
    34021 gcctttcggc gaggattatg tggcggcgca gcaggtcggc atcagtttgt cggggattct
    34081 ctatatgatt ccgcaaagcg tcggctcggc ggggacggtg cgcatcggct tttcgcttgg
    34141 gcggcgcgaa ttttcgcggg cgcgttatat ttcgggcgtg tcactggtgt taggatggat
    34201 gctcgccgtg attaccgtgc tttccttggt attattccgt tcgccgctgg taagtatgta
    34261 caacaatgat ccggcggttt taagcatcgc cgccaccgtc ttactgttcg ccggcttgtt
    34321 ccaaccggca gacttcaccc aatgtatcgc ctcctacgcc ttgcgcggct acaaagttac
    34381 aaaggtgccg atgttcatcc acgccgccgc cttttggggc tgcggtctgc tgccgggcta
    34441 cctgctcgcc taccgtttcg atatgggcat ttacggcttc tggacggcat tgattgcctc
    34501 gctcaccatc gccgccatcg ccttggtgtg gtgcttggaa ttgtgcagta gggagatggt
    34561 cagatcgcat aaggccgtct gaaaactccg ccgattaaaa atatagtgga ttaacaaaaa
    34621 ccagtacggc gttacctcgc cttgccgtac tatttgtact gtctgcggct tcgttgcctt
    34681 gtcctgattt ttgttaatcc actatagaaa cgatgggctg aagcccgcca ctggcaatac
    34741 tcattttaaa gttcaaaccc atgcaaccca tccgataccg aaccgacctt accccctaca
    34801 acactttcgg cctgcgcgcc caagcccgag cctttattgc gcttgaacac gccgacgggt
    34861 tgcgcgacat cgtccggcta cccgagttca accgagatac tgttttatgg ctgggcggcg
    34921 gcagcaatat ccttttgatg gaggactacg ccggtctggt cgtacatatg gaaaacaaag
    34981 gcatacgcga aattgctcgt tcagacggca tggttctgat tgaagcgcag gcgggcgaaa
    35041 tctggcacga ttttgtcctg cacgccgtcg cgctgggttt gaacggtttg gaaaacctga
    35101 gcctgattcc gggtacggtc ggcgcgtcgc ccgtgcagaa catcggcgca tacggcgtag
    35161 aggcgaaaga tgtgattcac agcgtgcgct gcttcgattt ggatacggaa acctttgtaa
    35221 ccctttccaa tgccgactgc cgcttcgcct accgcgaaag cctgttcaag caggaaggta
    35281 aagggcgtta tgtgattgtt tcggtcgtat ttgcattaaa aacgcatttt gtgccgaact
    35341 tgggttacgg cgatttggcg gccgccgttg ccgaactgag cgcgggcagg gtgccgacgg
    35401 cgaaagatgt ttccgatgca gtgtgtgcaa tccgcaacag taaacttcct aatcctaacg
    35461 tgcttggcaa tgtcggcagt ttctttaaaa accccgtcgt cagcgcagaa aaagccgcca
    35521 ccttgttgca gcggcatcct gatatgccgc gctatccgca gcccgacggt tcggtcaaac
    35581 tcgccgccgg ctggctgatc gaccaatgtc gtctgaaagg ctttcaaatc ggcggcgcgg
    35641 cggtacatga caggcaggct ttggtcttag tgaacaaaaa caatgcctcg gcaaacgatg
    35701 tccggcaatt ggcgcaacac atcaaattta cagtatttgc tcggtttcag gtagaattgc
    35761 acgccgaacc caattggctg cctgcttcgt tcagcctgta aatccaagga gtatgcccgt
    35821 gacccgcccc gccaaaatca atacttacac acgcatcatc gacgccagcc ttgcgctttt
    35881 caacgaggaa ggcgagcgca acatcagcac caaccatatt gccgcccact tgggcatcag
    35941 tccgggcaac ctctattacc acttccgcaa caaagacgaa atcatcgtcc aactgttcaa
    36001 acgttacagc gaagccctgc tggaatacct caacgaagcc gtgttgccgt ctgatgtgga
    36061 agactccatc aattatatgg cgggcattta cgacgtgatg tgggaatacc gcttcctatt
    36121 cagcgacgtg aacaccctgc ttgcacgcag tgccgaacta ttgggcgaac acaatacctt
    36181 cacccaagcc aaagtctccc cgctcttggt caacctactg acccagctca acggtctgaa
    36241 catcatccaa gccgaccaaa ccgccatgaa cgacctcgcc gtcaatatgt ggatgatcac
    36301 gaaatactgg ttcgacttcg acagctccct gcgcggtcgt accaagctga ccgaagactc
    36361 caaagcacgc ggcatccgcc gcaccttaag cctccttcgt ccttatcttt tgcccgaaca
    36421 ccgcaagaaa tacgaccgga aaatcggcaa cggcaacccg taaacccaca gcgtccgaat
    36481 cagccttcag acggcactta cccgacggta tcagcgaatg cgcgcagtcc ccccgcccca
    36541 tatctccatc acaggcctag gttacctcgg cctgccgctg gcacaaaagt tttaccaaca
    36601 cggcagccgc gtcgccgcca taaaacgcag cctgacttcg gacgacatca acctgcccat
    36661 acacctcgat accatcgacc tcaatcaaga cagcgcgttt caaagcgcga accttgcccg
    36721 agatacaagc ttttggcggc accatgccaa caaacccgtt tggttctgcc tcttgccgcc
    36781 atcatcgctg acacattacg ccgataccgt caaacaatgg gcagaacttg cccgggcgtg
    36841 caacgtgcaa cacctgattt tcacaagcag taccagcgtt tacggcgata cagcgcgcga
    36901 atgcgacgaa atcgccctac ccgatccgca aaccgagtcc gcccgccaaa tcctcgccgc
    36961 cgaacaacac ctgctcgaca gcggcgttcc gaacatcgac atcctgcggc tgggcgggct
    37021 ttattgcgcc gaacgccatc ccgtcagccg ccttgttcaa aagcaaaaca tcccgggcgg
    37081 caaccgcccc atcaacatcg tccaccgtaa tatcgccgtc gaaagcctgt ttcagacggc
    37141 atttaacccc agcggcaggc ggctgaaaaa cattatcgaa ccgcgccacc cgacacgccg
    37201 cgaatttttt acggaagaag ccgccaaact cggcctgccc gcgccggatt tcgcacccga
    37261 cgacagcgtg ggcaaaatca tccgtaccgt ttgcgataac ggcttaagcc tgtaaaataa
    37321 gcggcaacag caacaaacag gcattctccg ccccaatcag aaaatatgtc cgccttcctc
    37381 cccatcatca accgcctgat tctgcaaagc ccggacagcc gctcggaact tgccgccttt
    37441 gcaggcaaaa cactgaccct gaacattgcc gggctgaaac tggcgggacg catcacggaa
    37501 gacggtttgc tctcggcggg aaacggcttt gcagacaccg aaattacctt ccgcaacagc
    37561 gcggtacaga aaatcctcca aggcggcgaa cccggggcgg gcgacatcgg gctcgaaggc
    37621 gacctcatcc tcggcatcgc ggtactgtcc ctgctcggca gcctgcgttc ccgcgcatcg
    37681 gacgaattgg cacggatttt cggcacgcag gcagacatcg gcagccgtgc cgccgacatc
    37741 ggacacggca tcaaacaaat cggcaggaac atcgccgaac aaatcggcag attttcccgc
    37801 gaacccgagt ccgcaaacat cggcaacgaa gcccttgccg actgcctcga cgaaataagc
    37861 agactgcgcg acggcgtgga acgcctcaac gaacgcctcg accggctcga acgcgacatt
    37921 tggatagact aaccttcaga cggcatccga catgaacagc ccttttcaca acatcggcat
    37981 cgtaacccgc cccaacacgc ccgacatcca agacaccgca cacacgctga ttaccttttt
    38041 gaagcagcac ggctttaccg tctatctcga cgaagtcggc ataaaggaag gctgcatcta
    38101 tacccaagac accgtcggct gccatatcgt caacaagacc gaactggggc aatactgcga
    38161 cctggtcgcc gttttaggcg gagacggcac ctttctctcc gtcgcccgcg aaatcgccct
    38221 gcgcgccgtt ccgattatcg gcatcaacca agggcatttg ggcttcctga cccaaattcc
    38281 ccgcgaatat atgacggaca agctattgcc cgttttagaa gggaaatacc ttgccgaaga
    38341 gcgcatcctg attgaggccg cactcatccg cgaaggcaaa accgccgaac gcgccatcgc
    38401 cctcaacgat gccgtcctct cccgtggcgg cgctggacag atgattgagt ttgaagtctt
    38461 cgtcaatcgg gaattcgtct atacccagcg ttcggacggg ctgattgtct ccacccccac
    38521 cggatcgacc gcctattcgc ttgccgccgg cggccccatc atgcaggcag gattacacgc
    38581 cttcacactc gtacccatct gcccgcaatc catgaccaac cgccccatcg ccattcccga
    38641 cacgtctgaa atcgaaatcc tcgttaccca aggcggcgac gcgcgcgtcc atttcgacgg
    38701 tcaaacccat atcgacgtgc aaaacctcga ccgcatcacc atccgccgct accgaaatcc
    38761 cttacgcatt ttgcacccga ccgactacca atatttcaaa accctgcgcc aaaaactgca
    38821 ctggggcgaa caattggtct gaaccgaccc ttaccggaaa acacctatga atccaaaaac
    38881 tccccccgac catacagaag atgccgtgcg cctctccaaa cgcatggcgc aactggggct
    38941 ttgttcgcgc cgcgaagccg acggctacat cgaacagggc tgggtaacgg tcaacggcaa
    39001 aaccgccgta ctcggtcaga aagtttcacc ggcagaccgt atcgaactga acaagaaagc
    39061 ccacgaacag caggcggcac gcattaccat cctgttgaac aaacccgtcg gctatgtcag
    39121 cgcacaagcg gaaaaaggct ataaatccgc cgccgaactg attacccctg aaaatcactg
    39181 ggaaggcgat accggccgca tccgtttcga tccgaaacac aaaatcggcc tcgcccccgc
    39241 cggcaggctg gacatcgact cggtcggatt gctggtattg actcaggacg gccgtatcgc
    39301 caagcagctt atcggcgaaa acagcggcag tgaaaaagaa tatttggtgc gcgtgcgcgg
    39361 caaattggac gaaaaaggac ttgccttact gaatcacgga ttgagtttgg acggcgagaa
    39421 actgcgtccc gcccaagtag aatggcaaaa cgaagaccaa ctgcgtttcg tattgaaaca
    39481 aggtaaaaag cggcaaatcc gccgtatgtg cgaactggtc ggactgcgcg tcgtcgggct
    39541 gaaacgcatc cgcatgggta aggtcaagct cggcaggctg ccgcccggca aatggcgtta
    39601 tcttgctccc ggcgaatcgt tttaaataag cattaaaaaa tgccgtctga acgtccttac
    39661 gttttcagac ggcattgcct tatgcccaaa taacggtttc ttccaagggc ctgcgcgctt
    39721 tcgtggcgat ttcctgaacg cggtagccga atgtcgcggc aacggacacg ccccattctg
    39781 ccgcatcgaa caaaccgaac tgcccggaca atacgcgttc catatcggca tagttgaaac
    39841 cttccaccgg gcaggaatcg atacctgcca tcgccgcacc cgtcatcatg ttgcccaacg
    39901 cgatataggt ctggcggcaa caccagtcaa acaaggcgcg cgaatcgtcc aaaatcttga
    39961 tgtcgtcggc ttgaaacgcc tgatacctcg ccaaagattt tgccatggca tccggttcgg
    40021 taacgccgcg ccgtttgagg ctttccaaca taaacgggct gtcgaagcgg gcatttttct
    40081 ttgccaaaaa caccaccaaa tgactggcgg tatccaaagc atccgccata ccccaagaaa
    40141 acggcttgat tgcctgtcgg atttcggggt tttgaaccac aacaaactgc caaggctccg
    40201 aaccgaccga actgggcgac aaacgcccga gttctaaaat aaactgaaaa tcctcggcac
    40261 tgattttacg tgccgcatcg taatgccggc atgatttgcg gtttttaaat gcggatagaa
    40321 cctgctcttt gcttaatact gccatcgcac atcctttcat tacttgtatt gcaaattgat
    40381 ttcaaaccga tacggcactg ccgcaccata caatccgtcc tcgccatcaa gcttcatccg
    40441 gactttaccc atctctctac ggctgggaaa acaaagcggc ggcagactcg ggaataacgc
    40501 cccaatgcct ttcagacggc atttgggcat cattgcaaac gcgcctacgg gtaggcgata
    40561 taggcataag ccgaatggtt gtggatagac tcgaaattct cgctctcgac aacgaaactc
    40621 ttgatgcgtt tgtcggcgat cagcgaagta gcgacatcgc gcaccatatc ttccacgaat
    40681 ttcgggtttt cgtaggcctt ttcggtaacg tatttttcat cggggcgttt gagcaggccg
    40741 tagagttggc agctcgcctg cgcctccaca taatcgatga cttcctcgat accgacttcg
    40801 gcatcggcag tcaggctgac ggtaacgtgc gaacgctgat tatgcgcgcc gtattgggaa
    40861 atttctttgg aacacgggca aagcgaggtt acgggaatca tgaccttcat actgtggccg
    40921 tatgccccgt ctttgatttc gcccgtgaga cagacatcat aatccagtaa ggaccgaata
    40981 ccggaaaccg gcgcggtttt cttgcggaaa aacggaaaag aaacgctgat tttgccggcg
    41041 cgggaatcta aaagcgcgac catttcggta gtcagcttgc gcaattgtgc aaaatccaag
    41101 gcttcggcat gttgctccat caatgcgaca aaacgcgaca tatgcgtccc cttctgctca
    41161 gcaggcaggt aaaccgtcat ggtcagacgg gcaatggtgg actggatgcc ttctgcagtt
    41221 tgcagggtaa tcggaaagcg caggtctttg ataccgacct gattgatcgg cagattgcgc
    41281 aaatctcggc tggattgcac gtctgcaata gtgttcatga gttgtttttc cttaatattc
    41341 atgtaattgt tgagatgtgt gcatggtatg gcgtttccgc cggggaatat accgtcaatc
    41401 tgcaaatgcg agattatatc actcgctttt agcagctgca ttgattgttt ctatttttca
    41461 gatagtgata aaatcttcga ttattcacat ctacataggc agagaccaaa tgaaattcgc
    41521 cacgaaagcc attcattcca gctacgattg cgacgaacac aaccgcgcgc tgatgccgcc
    41581 gatttatcaa aacagtatgt ttgcgttgca cgagattggc gaaaatgtgc cttaccgtta
    41641 ttcgcgcctg agcaacccga cccgtcagat tttagaagac accgttgccg atttggaaca
    41701 cggtgcggca ggttttgcgt tttccagcgg tatggcggga attgatgccg tatggcgcac
    41761 tttcctgcgc ccgggcgata ccattgtcgc cgtcgccgat atttacggcg gcgcttatga
    41821 tttattggtc gatgtttatc aaaaatgggg ggtgaacgtt gtttttgccg atttaggcaa
    41881 tccggataat ttggacgaac tgcttaaagc gcacaaggtc aaactggttt ggctggaaac
    41941 gccgtccaat ccacttttac gcttggtaga catcaaagtc cttgccgcaa aagccaaagc
    42001 agccggtgcg ctggtcggta tcgacaacac ttttgccacg ccgtatctgc aacagccgtt
    42061 ggatatgggt tgcgattttg tattccattc cgctaccaaa tatttgtgcg gccattccga
    42121 cgtgttgatg ggcatcgtcg ttgccaaaac caaagaactg gcgcagcctt tgcacgatat
    42181 gatggtgcat accggcgcgg ttgccggccc gctggactgc tggctggtgt tgcgcggcat
    42241 caaaacgctt gccctgcgta tggaggcaca ctgcaaaaac gcactcgaaa tcgcgcgccg
    42301 tctggaagac catcctgcca ttgaaaaagt gttctatccc ggcctgccgt cgcacgaaca
    42361 ttatgaactc gcccaagcgc aaatgcctaa aggcatcggc ggcgtggtga cggtttatct
    42421 caaaaacgac acgcgcgaag cggcaaacag cgtgattaaa aacatgaaac tggtcaaaat
    42481 ggcttccagc ctcggcggcg tggaaagttt ggtcaaccat tgctattccc agtcccacag
    42541 cggcgtgccg cataatgtga aaatggaaat gggcatcaaa gtcggcctgc tgcgtttctc
    42601 catcggcatc gaagacgcgg acgatatttg gaacgatatt tccgccgcac tcgatacgac
    42661 tttgtaaacc gtaaaaatgc cgtctgaaac catagtttca gacggcattt caatattgcg
    42721 aatcaaatat caacgcttca gcatcttggc cgcctcaatg gcataatagg tcaaaatccc
    42781 atccgcgccc gcacgtttga atgccagcag gctttccaaa accactttat cgccatccag
    42841 ccatccgttg gcaaccgccg cctgtagcat cgcatattcg cccgaaacct gataggcata
    42901 agtcggcaca ccgaactcgt cctttacgcg gcggacaacg tccaaatacg gcaaaccggg
    42961 cttgaccatt accatatccg caccttcctg aatgtccaac gccacttcgt gcaacgcctc
    43021 atcggtattt gccggatcca tctggtaggt ctttttatct gccttgccca aattgcccga
    43081 actgcctacc gcatcacgga aagggccgta aaatgcagaa gcatatttgg cggaatacgc
    43141 cataatccgc gtatggatat gcccggcatc ctccaacgcc tcgcgaatcg caccgatacg
    43201 cccgtccatc atatcggaag gggcaaccac ctgcgcgccc gcttcagcgt ggcacaaagc
    43261 ctgcttgacc aaaacctcta cggtttcatc gttcatcaca taaccgtttt cgtccgtcag
    43321 cccgtcctga ccgtgcaccg tataaggatc gagcgcgaca tccgtcataa tgcccagttc
    43381 gggaaacctc tcacgcaagg ctcggacggc tgtcggcacc agtccttcag ggttatacgc
    43441 ctcctgcgca cattctgtct tatttgtcgt aacgacaggg aatagtgcca gcatgggtat
    43501 tcccaaattt gacgcttcct ccgccgtaaa cagcagcctg tccaaacttt gacgcttcac
    43561 acccggcata gaaggcacat cctcctcgcg cgccgacccc tccaatacga acaccggata
    43621 aatcaaatca tcggcggtca gcgtgtgttc gcgcatcagg cggcgtgaaa aatcgtccct
    43681 gcgcatacgg cgcatacgcg aagccggaac attgcggtaa ggaaactgca taagccctcc
    43741 aatcatcctg tcaacaattc aaaccatacg gaagccgccg caacttgcag acagcatcgg
    43801 gcggcaatca aaatccgttt tcaccggaaa gggtttcggc ccgccgaaat gccgcgccga
    43861 aggacggcgt cccgcccatt aaagaacaat agcaaaacaa cagcttcagg ctgtcagcct
    43921 tcgatattct tacgccacaa taccaaaagt ttcccgatat gctgaacagg ttgcgcgtca
    43981 accgcctcac ataaggcagt gcagatttcg atacgttcgg cacggtcgtc gccgaatacg
    44041 cgcactttaa tcagctcatg cgccgtcaat gccgcatcgg tttccttgat gaccgcgtcc
    44101 gtcagaccct gctgaccgac catcacaaca ggatggagat ggtgcgcgcg cgctttcagt
    44161 tccaaaattt ctttggtgtt caatttggta tccgtcattt tcctactgct tgaataaaaa
    44221 gaataatgcg gcattgtacg cgatttggca cgacatcggc aaaattaacg tacaatacgc
    44281 ggtttccatt ccgacccgcg aaccactatg gctgtacgtt ccaaatcctc aaaagcgtgg
    44341 ctgcacgaac acgtcaacga ccactacgtc catatggcgc aaaaagacgg ctaccgcgcc
    44401 cgtgccgcat acaaactttt ggaaatcaac gaaaaagaca aattaatcaa acccggcacg
    44461 gtacttgccg acttgggcag cgcaccggga agctggtcgc aggttgccgc caagctgacg
    44521 ggtacttccg gagcagtttt cgccttggac atcctgccta tggaagccat agggggcgtc
    44581 tccttcattc agggcgactt ccgcgagaac gacgtactgg cacaattcga aaccttgttg
    44641 gacaaccgcc cgctcgacct tgtaatttgc gatatggcgc ccaatatgtc gggaaacgcc
    44701 gtaagcgatc aggcacgcag cttttatctg tgcgaactgg ctttagactt cgcctcgcaa
    44761 cacctgaaaa ccggcggcag ctttttggtc aaagtctttc agggtgcagg ctatcaggaa
    44821 tacatggcag ccatgcgcga aattttcggc acggtgcaga cgcgcaaacc cgaagcctcg
    44881 cgcaatcgct ccagtgagat ttatttattg ggcaaaaata aacgctgaca atacagacgg
    44941 cgtgctttac aatcatttcc gttttacacc tcaattatgg agccttgcta agtggggaac
    45001 acctttaaat caatccttgt ctgggtcgcc ttgggtatcg gcctgatggc tgcgttcaac
    45061 gctttagacg gtaaaaaaga agacaacggg caaatcgaat attctcagtt catccaacag
    45121 gtcaacaacg gcgaagtatc cggcgtcaac atcgaaggat ccgtcgtcag cggctacctg
    45181 attaagggcg agcgcaccga caaaagcacc ttcttcacca acgcgccttt ggacgacaac
    45241 ctgattaaaa cactgctcga caaaaacgtc cgtgtaaaag taacgccgga agaaaaaccg
    45301 agcgcgctgg ctgccctgtt ttacagcctg ctgcccgtcc tgctgctgat tggcgcgtgg
    45361 ttctacttta tgcgtatgca gacgggcggc ggcggaaaag gcggcgcatt ctcattcggc
    45421 aaaagccgcg cacgcctgct ggacaaagat gccaacaaag tgacctttgc cgatgtcgcc
    45481 ggctgcgacg aagccaaaga agaagtgcag gaaatcgtcg attacctcaa agcaccgaac
    45541 cgctatcaaa gcttgggcgg gcgcgtgccg cgcggcatcc tgctggcggg cagcccgggt
    45601 acgggtaaga cgcttttggc aaaagccatt gcaggcgaag ccggcgtgcc gttcttcagc
    45661 atttcaggtt ccgactttgt cgaaatgttc gtcggtgtcg gtgcgagccg cgtccgcgat
    45721 atgttcgagc aggcgaagaa aaacgccccc tgcatcatct ttatcgacga gattgacgca
    45781 gtcggccgcc aacgcggcgc aggtttgggc ggcggcaatg atgagcgcga gcaaacatta
    45841 aaccaattgt tggttgaaat ggacggtttt gagagcaatc agactgtaat tgtgattgcg
    45901 gcaaccaacc gccccgacgt actcgatcct gcgctgcaac gccccggccg tttcgaccgc
    45961 caagtcgtcg tccccctgcc ggacatccgg gggcgcgaac agattttgaa cgtccattct
    46021 aaaaaagtgc ctttggacga atctgtggat ttattgtccc tcgcgcgcgg cacgccgggt
    46081 ttttccggtg cggatttggc aaacctggtt aacgaagccg ccctgtttgc cggccgccgc
    46141 aacaaagtca aagtcgatca aagcgatttt gaagacgcaa aagacaaaat ctatatgggt
    46201 ccggaacgcc gcagtatggt gatgcacgaa gacgaaaaac gtgcgacggc gtatcatgag
    46261 tccggacacg cgattgttgc cgaaagcctg ccctttaccg accccgtcca caaagtaacc
    46321 attatgccgc gcggacgtgc gctgggtctg acttggcagc ttccggagcg cgaccgcatc
    46381 agtatgtata aagatcagat gttgagccag ctctccatcc tgttcggcgg acggattgcc
    46441 gaagacatct tcgtcggacg catctccacc ggcgcatcaa acgactttga acgcgcaacc
    46501 caaatggcgc gcgaaatggt aacgcgctac ggcatgagcg acaaaatggg cgtgatggtt
    46561 tatgcggaaa acgaaggcga agtcttctta ggacgcagcg taacccgttc gcaaaacatt
    46621 tccgagaaaa cccagcagga catcgatgcg gaaatccgcc ggattttgga cgagcagtat
    46681 caggttgcct acaaaatcct cgatgaaaac cgcgacaaga tggaaaccat gtgcaaagcc
    46741 ctgatggaat gggaaaccat cgaccgcgat caggtactgg aaatcatggc gggcaaacaa
    46801 cccagtccgc ccaaggatta cagccacaac ctgcgcgaga atgcggacgc ggcggaagat
    46861 aacgcgccgc acgctccgac tcgggaagaa accgaagcac ctgccccggc agacaccgct
    46921 tcgacagagt ccgagcagca gcctgaaaac aaggcttaac ttcccgaaca aacggcagcc
    46981 cgcaagctgc cgttttccca tcctgttttc agacggcatt ttccgctcaa tgccgtctga
    47041 aacgccaaag ccgtatcggt ttttgcggat ctgcgatatg gcggtttaaa ttgaaatcag
    47101 acgaggcaag gctgtaccgg tttaaaatta aaccgctata ataccgccct tcgaatacac
    47161 gcccgcattg gtgatgcctg tgaaaaaagt tgaaaaaaac atcttggtgc tgcacggcgc
    47221 ggacaaaatg ttcgagctgg tcgataaggt tgaagactat ccgcactttc tgccgtggta
    47281 cagcaagacc gaggtcatcg ggcgtagcgg caacgaactg aaggcgcggc tgtttatgga
    47341 ctatatgcac gttcgccaat cattcgccac gcacaaccgc aacattccgg gcagggaaat
    47401 ccgtatggaa ctgctcgagg gtccgttcaa aaccttacgt ggaacgtgga aatttatcga
    47461 tttgggcgac gatatgtgca aaatcgaatt caatttggaa tacgattttt ccaatgccgt
    47521 tttgtccgcc ttaatttccc ccgtcttcaa ccacctttcc gtcacgctgg tcgaagcgtt
    47581 cgtcaaagag gcagaccgcc gttatgcttg aaattgagat tgtgtacggg ctgcccgacc
    47641 gacaggtttt gaaaaccatg cagcttgccg aaggaacaac cgtccgcgcc gccgcactgc
    47701 aaagcggttt ggacggcata tttgaagatt taaacctgca ttccgcgcct ttgggcattt
    47761 tcggcaaagc cgtcaaagac gacacgccgc tgcgcgacgg cgaccgcatc gaagtgtacc
    47821 gcccgctgtt gatcgacccc aaagaagcgc gccgcaaacg cgttcaaaat caagaagaat
    47881 aaccatgccg tctgaagcct tcagacagca ttcgacagaa acacggaaaa tatcatgtca
    47941 aacacaatca aaatggttgt cggcttgggc aacccgggca aagaatacga acagacacgc
    48001 cacaatgcgg gtttttggtt cctcgacgaa ctggcgtgga aatggaaggc ttcatttaaa
    48061 gaagaaaaaa aattcttcgg cgaagtcgcc cgtgccgccc tgcccgacgg cgacgtttgg
    48121 ctgctcaaac ctgccacgtt catgaaccgt tccggacagg cagttgccgc ccttgcgcag
    48181 ttttataaaa tcaaacccga agaaatcctc gtcgtccacg acgaactcga cattccctgc
    48241 ggacggatca aattcaaact cggcggcggc aacggcggac acaacggctt gaaagacatt
    48301 caggcaaaac tcggcacggc agactattac cgtctgcgcc tcggcatcgg ccacccgggc
    48361 gaccgcaacc tcgtcgtcgg ctatgtcctg aacaaaccca gtacggaaca ccgccgacag
    48421 attgacgatg ccgtcgccaa atccctgcag gccatacccg acatccttgc cggcaaatgg
    48481 gaagaggcaa cccgcttcct gcacagcaaa tgacccgatg ccgtctgaag ccctttcaga
    48541 cggcatgttc ccgatttcca tatccgaaca gtcatgaccg aactcaagca gcttatccaa
    48601 accgaatcca tccccgtcat cgaagaaacc ctcgatttcc tgctgtacga atgcagcatc
    48661 gacgacgcac catccgccga agaagtggca caatggcgcg acatacttgc cgcacgcggc
    48721 ggcaaattcc tgcgcctgtc caaaatctgc caaacgtggc tggacgagga ggcggcatga
    48781 agctgccgcg caaccgcttc agcctgcttt ccgcattgtg gtttgccggc ggcatctatt
    48841 cgctgctctt caaagctgcc gacaccgcgc cgccgccgtt tccgcatttc gacaaagcag
    48901 cacaccttgc cctgtttttc gcacaaatct ggcttttgac caaagcattc aaaaccggaa
    48961 aacttcccat cccctaccgc agcctgatgg tctttgccct ctgtttcgcc ctcttcagcg
    49021 aatgcgcgca ggcatggttt accgcaacga gaaccggcag tttgggcgat gttcttgccg
    49081 atatggcagg tacggttctc gcactctttg ccgcccgcgc cgccgaccgc ccggactgaa
    49141 tcggtttatt tcccccaaac aggatgcacc tcttcccgat acatcctatt tttcccctta
    49201 catatcaatc ccatctcata aacaaaaccg acaaatcgtt tacaatatat ttacactaca
    49261 tcagattaca aatatactcg aaccagttgc agaagcggcg attccacaaa ccgtttcgga
    49321 ttgcacgaca caaaagaaaa accgattttg ttgccttaaa ggagtattca tgaacctgca
    49381 tgcaaaggac aaaacccagc atcccgaaaa cgtcgagctg ctcagtgcgc agaagccgat
    49441 taccgacttt aagggcctgc tgaccaccat tatttccgcc gtcgtctgtt tcggcattta
    49501 ccacatcctg ccttacagcc ccgatgccaa taaaggtatc gcgttgctga ttttcgttgc
    49561 cgcactttgg tttaccgaag ccgtccacat taccgtaacc gcactgatgg tgccgattct
    49621 cgccgtcgta ctcggtttcc ccgacatgga catcaaaaag gcgatggctg atttttccaa
    49681 cccgattatc tacatttttt tcggcggctt cgcgcttgcc accgccctgc atatgcagcg
    49741 gctggaccgt aaaatcgccg tcgccctgct gcggctgtcg cgcggcagta tgaaggccgc
    49801 cgtgctgatg ttgttcctcg ttaccgcctt tctgtccatg tggatcagca acaccgccac
    49861 cgccgcgatg atgctgcctc tggcaatggg tatgctgagc cacctcgacc gggaaaaaga
    49921 acgcaaaacc tacgtcttcg tcctgctcgg catcgcctat tgcgccagca tcggcggctt
    49981 gggcacgctc gtcggctcgc cgcccaacct gattgccgcc aaagccctaa atctggactt
    50041 cgtcggctgg atgaagctcg gcctgccgat gatgctgttg attctgccct tgatgctgct
    50101 ctccctgtac gtcatcctca aacctaattt gaacgaacgc gtggaaatca aagccgaatc
    50161 catcccttgg acgctgcacc gcgtgatcgc gctgttgatt ttccttgcca cagccgccgc
    50221 gtggatattc agctccaaaa tcaaaaccgc cttcggcatt tccaatcccg acaccgttat
    50281 cgccctgagt gccgccgtcg ccgtcgtcgt cttcggcgtg gcgcaatgga aggaagtcgc
    50341 ccgcaatacc gactggggcg tgttgatgct cttcggcggc ggcatcagcc tgagcacgct
    50401 gttgaaaaca tccggcgcgt ccgaagcctt gggacagcag gttgccgcca ccttttccgg
    50461 cgcgcccgca tttttggtga tactcatcgt cgccgccttc attatttttc tgaccgagtt
    50521 caccagcaac accgcctccg ccgcattgct tgtaccgatt ttctccggca tcgctatgca
    50581 gatggggctg cccgaacaag tcttggtatt cgtcatcggc atcggcgcat cttgtgcctt
    50641 catgctgccg gttgccacac cgcctaacgc gattgtgttc ggcacgggct taatcaagca
    50701 acgcgaaatg atgaatgtcg gcatactgct gaacatcctc tgcgtagtat tggttgctct
    50761 gtgggcttat gctgtactga tgtaaaccat cgacctaaac aacaagaccg tctgaaagaa
    50821 tatttttcag acgaccttga agtttgtcgt tcagacacaa tttgtcgaat cattcaaaac
    50881 cggattctaa cgaaaggaaa cccatgatta tcctgcacac caacaaaggc gacatcaaaa
    50941 tcgaactcga tttcgacaaa gcccctgtta ccgccaaaaa cttcgagcaa tacgtcaaag
    51001 acggcttcta cgacggcgta atcttccacc gcgtcatcaa aggcttcatg attcaaggcg
    51061 gcggcatgga tgaaaacatg aacgaaaaag aaacccgcga tccgattcaa aacgaagcgt
    51121 ccaacggcct gcccaacgat aaatacacca tcgccatggc acgcacttcc gacccccatt
    51181 ccgccagcgc gcaattcttc atcaacactg ccgacaacgc tttcctgaac ttccgttcta
    51241 aagagctgta cggcaaaacc gtcgtccaag actggggcta tgccgtattc ggtaaagtcg
    51301 ttgacggttt tgacgttgtc gatgccatcg aaggcgtttc taccaaacgt catggttacc
    51361 acgacgacgt accgagcgaa cctgtcgtca tcattaaagc cgaagcggta taaaccgaca
    51421 atccgaaagc agcctgcaca aaggctgctt ttatttcaga cggcatgtta tttgatgtaa
    51481 ccgtgctatg ccgtctgaaa accggacgca agccttcaga cggcataccg ctatgttaaa
    51541 atatgcccgt ttttcctaaa cggacacaaa ggaaacctta tggcaaatat tgcccgcgac
    51601 atcttcaaag cctacgacat ccgaggcatt gtcggcaaaa ccctgaccga cgatgccgcc
    51661 tatctcatcg gcagggccat cgccgccaaa gccgccgaaa aaggcatgac ccgcatcgcg
    51721 ctcggacgcg acggacgctt gagcggtccc gaactgatgg agcacatcca acgcggcttt
    51781 accgacagcg gcatcgacgt cctcaacgtc ggtatggtcg ccacgcccat gctctatttt
    51841 gccgccatca acgaatgcgg cggcagcggc gtgatgatta ccggcagcca caatccgccc
    51901 gattacaatg gtttcaaaat gatgctcggc ggcgacacgc tcgcaggcga agccattcaa
    51961 gaacttttag ccattattga aaaagacggt tttgttgccg ccgacaaaca aggcaacgta
    52021 accgaaaaag acatctccgg cgaataccac aaccacatcg tcggccacat caaactcaaa
    52081 cgccccatga aaatcgccat cgacgcaggc aacggcgtag gcggcgcatt cgcaggcaaa
    52141 ctctacaaag gcttgggcaa tgaagttacc gaacttttct gcgaagtgga cggcaacttc
    52201 cctaatcacc accccgaccc ttccaaaccg aaaaacctgc aagacctcat cgtcgagctc
    52261 aaaaacggcg aggccgaaat cggcttagca tttgacggcg atgccgaccg cttgggcgtg
    52321 gttaccaaag acggcaacat catctatccg gaccgccaac tgatgctttt cgcgcaagac
    52381 gtgttgagcc gcaatcctaa agccaaagtg attttcgacg tcaaatccac ccgtctgctt
    52441 gcgccttgga ttaaagaaca cggcggcgaa cctgtgatgg aaaaaaccgg ccacagcttt
    52501 atcaaatccg ccatgaaaaa aaccggcgcg ccggttgccg gcgaaatgag cggacacatc
    52561 ttcttcaaag aacgctggtt cggcttcgac gacggtctgt acgccggcgc acgcctcttg
    52621 gaaatcctgt ctgcctccga taatccgtcc gaagtgttaa acaacctgcc gcaaagcatt
    52681 tccacgcccg aactcaacat cgccctgccc gaaggcagca acggccatca ggttatcgac
    52741 gaactcgccg ccaaagccga atttgaaggc gcaaccgaaa tcatcaccat cgacggtctg
    52801 cgcgttgaat ttcccgacgg cttcggtctg atgcgtgctt ccaataccac gccgattctg
    52861 gtgttgcgtt ttgaggcgga tacgcaagaa gccatcgagc ggattcaaaa ccaattcaaa
    52921 gccgtcatcg aaagcaatcc gaatctaatc tggcctctgt aaacacagga aaaatgctta
    52981 atcaaatctt ggtagattgg atttggctta aaaatctccg cgtccgttcg tgtttggggt
    53041 ggtcgaacaa ctctttcggg ctgccctgct ctacgataac gccgccgtcc atcacgacaa
    53101 cggtcgtggc aacttccagc gcgaacttga tttcgtgggt aacgacgacc atcgtccaac
    53161 cttcccgcgc caattccttc atggcgttca acacgtcttg caccaactcg gggtcaagcg
    53221 cggaagtggg ttcgtcaaac aacatcagct cgggctgaat cgccaatgct cgggcaatgc
    53281 cgacgcgctg ctgctgaccg ccggaaagct ggtagggata caaatccact ttgtcgccca
    53341 agccgacttt ttccagcagt ttcagagcct cttcgcgcgc ttgggcggca ggcttgccct
    53401 gtacggcaac cggtccttcc atcacgtttt ccaaggcggt tttgtgcgga aagaggttgt
    53461 attgttgaaa caccatgcct gatttgcggc gcagtgccaa aatatcgtgt ttgcttggtt
    53521 ttttagaaaa atcgattttc agcggtcgct cgttgtcgaa ctcgatttgt ccgtcttcgg
    53581 gcatttccaa cgcgtttagg catcgcagaa acgtcgtttt acccgagccg gaaggcccga
    53641 ggatgacgac cacctgccct ttgcacacat ccaaattgat gccgcgcaaa atggtatttt
    53701 tgccgaaggt cttatggata ttgcggattt taatcatgac aactccttat ttggcgacat
    53761 agcggtcgaa gcgtttttcc aagcgcgcct gaatcaggaa cagcactttg caaaaacacc
    53821 agtaaaccaa agcggcttcg atatagacgg gcaaaaagtc ataagtgcgg tttgccgttt
    53881 cctgcgcgac gcggaataat tccgttaccg tcacgactgc cgcgagcgag gtgtttttaa
    53941 acaaaccgat aaactcgttg ctcaaaggcg gcacggcaac gcgaaatgcc tgcggcgcga
    54001 cgatgcggcg gaacgtctgc atataggtca tgccgatgga gaaacctgct tcccattggc
    54061 ctttcggtac ggacaaaatt gccgcgcgta tggtttcgga agcatatgcg ccgacattga
    54121 gcgaaaagcc gatgatggcg gcaggaatcg ggtcgatata gatgccgacg gaaggcagcc
    54181 cgtaaaacac aatcacaagc tgaaccaaca gcggcgtacc gcgaatgacg gaaatataaa
    54241 attccaccaa tttcagcagg attttccgca cgatgccgcc ggcgggcata atccgcacca
    54301 aagccacggc taccgcaatc atcataccga taacgaaaga agctgccgcc aaaggcagag
    54361 agaccgcgaa gccggctttg accataggca aaaacgcgct gacaatcata tcggcgcgtg
    54421 tttccgtcat aaacggcagc gaagcaagga aattattgaa cactgatgtc ttttccgaag
    54481 aattgttcgc ccagtttttt cagcgtaccg tcggctttca gctcgttgat tgccgtactg
    54541 aatttcgcca cggcttcgtc attgcccttg ttgacaatca ggccggaacc gactttttca
    54601 tcggcaggtg cggaccaaac gattttcacg cccgcattcg ggtttttctt cagatagtcc
    54661 aaaaccgcca attcgtcgtt cagggttgca tcggcacgtt tttgttcaat cagggtcagc
    54721 gattgcgcca aaccgtcaac agccaccaaa tctgcgcctg cagctttggc tttttcgccg
    54781 tagttgctgg tcagggattg tgcggttttc acgcctttga tgtcgtcgat agatttgatg
    54841 ttgctgtcgt tacgggcaac caatacggca ccgctccagc tgtaaggatc ggatttgtcg
    54901 aatgtcgctt ggcgttcggg gctggtcaga ccgacttggt ttgccaccac gtcgaaacgc
    54961 cccgccttca aacccgccat catcgaatcc cattgcgttt ctttaaactc gactttcacg
    55021 cccagttttt ccgccacggc gcgggttact tccacatcgt aaccggtcag tttgccgtcc
    55081 ttgtcgtggt aggtaaacgg tgcgtaagtg ccttccgtgc cgacggtaac cgtgccttta
    55141 ttgttgatgc gctcgattaa agaaccggaa actgccgatt gtgcaggcgc ggaagatgct
    55201 ccgctgccgc tttccgaacc gccgcaggcc gccaaaacca atgcggcaat gccgccgagt
    55261 acgaattttt tcaacatcat tctctcctgt aaaaagtgtg caagtgcggc attctaagtg
    55321 aaattgcggc aaacacaaag aatacttttt ttatatggtt attccaaatc ggaatggaaa
    55381 agatatgccg aagccgccgg aagcggaaat aaagggggcg ggaaaggcga cgggcggcac
    55441 gaaaccgaaa agcggcatca ggtatgcaaa cggcggattc acgcacgccg ttccggcaaa
    55501 aatccgcctg ccgattcggt gctataatcc atccgaaaca gatgacacca aaaaggaaca
    55561 tctcatgaac acgccccaat ccgccatcat tcccgaccac gcccaagccg gcatctttat
    55621 cgaagccgac ttcgccgcca accgcctcaa cgatattaaa gccgcctgcc gcgcttcgct
    55681 cgacgcgtta tccgccttga aaacccgctt tccggacgat attttgggtc tgaccatcgc
    55741 cttcggcagc aaagcctggg caacattcgg acatacggac gaaggcagtg aaatcaaacc
    55801 cttctccgaa atgggcaacg ggcttgcgcc gtccacgcag cacgatatgt ccatccacat
    55861 ccaatctttc cgccaaaacg ccgcctacgc gcttgcccaa tccgttttgg gcgcgttcgg
    55921 cgacagcata tgcgtcgcgt ccgaagaaca cggtttgcgc ctgtatcagg atcgcgggct
    55981 ggacggtttc gtcgacggca cggaaaaccc gcagggcgat gaaaatgtcc gcgaagtcgc
    56041 catcatcccc gaaggaaaac ccgatgcggg cggcagctat gtcctgctgc aaaaatacct
    56101 gcacgatttg aaaaaatggg atgccgtccc cgtcgccgaa caggaggcct cggtcggacg
    56161 cggcaaggaa accgacgacg aattcggcca cgatgtccgc ctgcccgatt cgcacctcgg
    56221 ccgcgtcaac ctgaaagaaa acggagtcgg cctgaaaatc gtccgccgca gcctgccctt
    56281 cggcaaaatc agcggcgaac acggtttgat gtttaccgca tactgccgca cgctgcacaa
    56341 tatcgaagcg caattattga gtatgttcgg cgacacggat ggcaaaaccg acctgctgct
    56401 ccggcacctc tccgccgccg tttcgggcgg atactactac gcgccgtctg tcgaacgcct
    56461 gcaaaacctc taagccagac gatgccgtct gaaaccattt ttcagacggc attttcc
//
LOCUS       DAWFAN010000002        35715 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWFAN010000002 DAWFAN010000000
VERSION     DAWFAN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.002"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(127..1989)
                     /gene="hscA"
                     /locus_tag="WGO65_000056"
     CDS             complement(127..1989)
                     /gene="hscA"
                     /locus_tag="WGO65_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ0326428.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            2160..3032
                     /gene="hpnD"
                     /locus_tag="WGO65_000057"
     CDS             2160..3032
                     /gene="hpnD"
                     /locus_tag="WGO65_000057"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ0326429.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            3111..4424
                     /locus_tag="WGO65_000058"
     CDS             3111..4424
                     /locus_tag="WGO65_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0326430.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNPLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPYYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            4508..5227
                     /locus_tag="WGO65_000059"
     CDS             4508..5227
                     /locus_tag="WGO65_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0326431.1"
                     /translation="MATLTDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            5392..6063
                     /locus_tag="WGO65_000060"
     CDS             5392..6063
                     /locus_tag="WGO65_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ0326432.1"
                     /translation="MKTVSTAVVLAAAAVSLPGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            6073..6441
                     /locus_tag="WGO65_000061"
     CDS             6073..6441
                     /locus_tag="WGO65_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ0326433.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGDNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            6438..7085
                     /locus_tag="WGO65_000062"
     CDS             6438..7085
                     /locus_tag="WGO65_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ0326434.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            7374..8517
                     /locus_tag="WGO65_000063"
                     /pseudo
     CDS             7374..8517
                     /locus_tag="WGO65_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(8627..8836)
                     /locus_tag="WGO65_000064"
     CDS             complement(8627..8836)
                     /locus_tag="WGO65_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326435.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(8833..9129)
                     /locus_tag="WGO65_000065"
     CDS             complement(8833..9129)
                     /locus_tag="WGO65_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ0326436.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(9108..9713)
                     /locus_tag="WGO65_000066"
     CDS             complement(9108..9713)
                     /locus_tag="WGO65_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326437.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNAIETAPGLDALEKEIEEWTLNIG"
     gene            9793..10080
                     /locus_tag="WGO65_000067"
     CDS             9793..10080
                     /locus_tag="WGO65_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ0326438.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            10052..10354
                     /locus_tag="WGO65_000068"
     CDS             10052..10354
                     /locus_tag="WGO65_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ0326439.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(11063..13036)
                     /locus_tag="WGO65_000069"
     CDS             complement(11063..13036)
                     /locus_tag="WGO65_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ0326440.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGAAADNAHIVFGDNTLRLHGANNRISYNNHDIFHKANKPRFAEDIKGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTQPGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPAGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDTA
                     AVGYEDHNERQRSALVSTWTDGDLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(13047..13607)
                     /locus_tag="WGO65_000070"
     CDS             complement(13047..13607)
                     /locus_tag="WGO65_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ0326441.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPSSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(13604..14659)
                     /locus_tag="WGO65_000071"
     CDS             complement(13604..14659)
                     /locus_tag="WGO65_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0326442.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLRRRNPTTASGTLTVSGIAQSMLSDG
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADELGAAANVRDGEAQLMAAPAGVSTECR
                     LTVQGGTDRESDASLLARLLEIIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLG
                     TVDIAITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGID
                     LDDAKRRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVN
                     RIEWFKAGAINVTEMPS"
     gene            complement(14673..15020)
                     /locus_tag="WGO65_000072"
     CDS             complement(14673..15020)
                     /locus_tag="WGO65_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ0326443.1"
                     /translation="MDKELNPGTGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            complement(15120..15788)
                     /locus_tag="WGO65_000073"
     CDS             complement(15120..15788)
                     /locus_tag="WGO65_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ0326444.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDFEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(15785..16930)
                     /locus_tag="WGO65_000074"
     CDS             complement(15785..16930)
                     /locus_tag="WGO65_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0326445.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(16920..18251)
                     /locus_tag="WGO65_000075"
     CDS             complement(16920..18251)
                     /locus_tag="WGO65_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ0326446.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGAVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAVAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAAINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDMARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(18251..20422)
                     /locus_tag="WGO65_000076"
     CDS             complement(18251..20422)
                     /locus_tag="WGO65_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ0326447.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPDTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALGWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            20577..21159
                     /locus_tag="WGO65_000077"
                     /pseudo
     CDS             20577..21159
                     /locus_tag="WGO65_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21203..21472)
                     /locus_tag="WGO65_000078"
     CDS             complement(21203..21472)
                     /locus_tag="WGO65_000078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326448.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(21412..21777)
                     /locus_tag="WGO65_000079"
     CDS             complement(21412..21777)
                     /locus_tag="WGO65_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326449.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            complement(21781..22158)
                     /locus_tag="WGO65_000080"
     CDS             complement(21781..22158)
                     /locus_tag="WGO65_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0326450.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(22223..23632)
                     /locus_tag="WGO65_000081"
     CDS             complement(22223..23632)
                     /locus_tag="WGO65_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ0326451.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(23635..23832)
                     /locus_tag="WGO65_000082"
     CDS             complement(23635..23832)
                     /locus_tag="WGO65_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ0326452.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(23829..24479)
                     /locus_tag="WGO65_000083"
     CDS             complement(23829..24479)
                     /locus_tag="WGO65_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ0326453.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(24469..24891)
                     /locus_tag="WGO65_000084"
     CDS             complement(24469..24891)
                     /locus_tag="WGO65_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ0326454.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(24891..25289)
                     /locus_tag="WGO65_000085"
     CDS             complement(24891..25289)
                     /locus_tag="WGO65_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326455.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            complement(25354..26262)
                     /locus_tag="WGO65_000086"
     CDS             complement(25354..26262)
                     /locus_tag="WGO65_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ0326456.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            complement(26262..27323)
                     /locus_tag="WGO65_000087"
     CDS             complement(26262..27323)
                     /locus_tag="WGO65_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326457.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAEGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            complement(27554..27970)
                     /locus_tag="WGO65_000088"
     CDS             complement(27554..27970)
                     /locus_tag="WGO65_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0326458.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            complement(28098..29423)
                     /locus_tag="WGO65_000089"
     CDS             complement(28098..29423)
                     /locus_tag="WGO65_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ0326459.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLGNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            complement(29410..30978)
                     /locus_tag="WGO65_000090"
     CDS             complement(29410..30978)
                     /locus_tag="WGO65_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0326460.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHLSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            complement(30978..32600)
                     /gene="terL"
                     /locus_tag="WGO65_000091"
     CDS             complement(30978..32600)
                     /gene="terL"
                     /locus_tag="WGO65_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0326461.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFGNEIKSKWSR"
     gene            complement(32605..33429)
                     /gene="dcm"
                     /locus_tag="WGO65_000092"
     CDS             complement(32605..33429)
                     /gene="dcm"
                     /locus_tag="WGO65_000092"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0326462.1"
                     /translation="MDKQKPTVGSLFAGIGGFDLGFEQAGFDTAWQVEINPVPRAVLT
                     DRFPYAKQFEDVRTVLSELWPVDVIIGGFPCQDVSIAGKRKGLAGERTGLFYDAMRIV
                     DQLKPRWIVLENVTGLLNSNNGEDFQTVIKSLAECGYVGYWRVLNAAYFGVPTARRRV
                     FLVAGLGEYPPPYEFMADAGSIGVLPRSVDTCGLQKPHGTLLRGISNGAIDRSGANIL
                     VTPNGRGAMVERQRASNHDGLLLGMDEANTAEAQAAGNAVCPPVSRWIAEKLIKTF"
     gene            complement(33422..33928)
                     /locus_tag="WGO65_000093"
     CDS             complement(33422..33928)
                     /locus_tag="WGO65_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0326463.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRASYTLADGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(33931..34128)
                     /locus_tag="WGO65_000094"
     CDS             complement(33931..34128)
                     /locus_tag="WGO65_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326464.1"
                     /translation="MSAPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(34125..34391)
                     /locus_tag="WGO65_000095"
     CDS             complement(34125..34391)
                     /locus_tag="WGO65_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326465.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(34403..34741)
                     /locus_tag="WGO65_000096"
     CDS             complement(34403..34741)
                     /locus_tag="WGO65_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326466.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYNTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            complement(34738..34956)
                     /locus_tag="WGO65_000097"
     CDS             complement(34738..34956)
                     /locus_tag="WGO65_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326467.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            34969..35139
                     /locus_tag="WGO65_000098"
     CDS             34969..35139
                     /locus_tag="WGO65_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ0326468.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(35184..35489)
                     /locus_tag="WGO65_000099"
     CDS             complement(35184..35489)
                     /locus_tag="WGO65_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326469.1"
                     /translation="MSLLKTVKMQAAVVLTALALTACSSTSAPSATEIKVVEKAVLPT
                     PPAALMVAPVRPNPPKDGKTVTLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(35386..>35715)
                     /locus_tag="WGO65_000100"
     CDS             complement(35386..>35715)
                     /locus_tag="WGO65_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326470.1"
                     /translation="GYKSAFAKQQAVIDKMEKDKAQALLLSAQNYARELEQARAEAKK
                     YEVKAHAVGMALAKKQAEVSRLKTENKKEIENVLTQDRKNAGGGCIDGFGSHGLQLYK
                     RALGYGN"
BASE COUNT         7404 a        10135 c         9469 g         8707 t
ORIGIN      
        1 acgcccttga tgacggtttt catcgaatag acaatgcctg acttttccat atcgatgttt
       61 ttcaagtgtt ttcgagcctt cagacggcat caggttatat ctatgccgtc tgaaaccgtt
      121 taagtatcaa atattatcga cactctggcc tgtcaatgcg cgttggatat tgcggttcat
      181 gcgtttggcg gcgaagttgt cggtgctgcg gctgagtttg gcgacggcgg agcggatgtc
      241 ttcggctttt ccgtctttca gacggccttg caaatcggcg atgccttgcc ggatctgttg
      301 caattctttg gcatccagta aatcgctgtc caactcgagg gcggcgttga cggcatcggt
      361 caggctttcg gcttcgacta cggcttcggc gcgggcgcgt gccgccatat cttcggcggc
      421 gttgtccata ctgtctttaa gcattcgggt aatcgcgccg tcgtccaagc cgtaggaagg
      481 tttgacttcg atttgcgcct gtacgccggt gctttgttct tgggcggaaa cggacagcag
      541 cccgtccgca tcgatttgga aggttacgcg gatacgcgcc gcacccgccg ccatcggtgg
      601 aatgccgcgc agggtgaatt tggcaaggct gcggcagtca gaaacgagct cgcgctcgcc
      661 ttgcacgacg tgtatcgtca tcgcggtttg accgtctttg aaggtggtaa agtcctgcgc
      721 gcgcgcggtg gggatggtgg aattgcgcgg gatgattttt tccgccaagc cgccgtaggt
      781 ttccaaaccg agcgacaaag gtgtaacgtc cagcagcagc cattcgccgt cggttttgtt
      841 gcctgcgagg acgtttgcct gtatggcggc gccgagcgcg acgacttcgt cgggattgag
      901 gttgttcagc ggggtttgtc cgaagaaggt ggcgactgcc tgttggacgt gcagcatacg
      961 ggtcgaaccg ccgaccataa tcacgccttt gacttcgttt ttaccgacac cggcatcttt
     1021 caacgcctgt gtgaccggtt cgagcgtttt catcaccaaa tgctgcgtca ggttgtggaa
     1081 ctcggcgcga ctgatgcttg tgtcgattgc catgccgtct gaaagcgtcg cctgaatgcg
     1141 cgcttcggtt tgcgtggtta attgttcttt ggcggcgcgg acgagcgaga gcaggagttg
     1201 gctgtcttgt tcgttgagtt gggagagtcc gttttgttcg agcaggcggc agaacaggcg
     1261 gtggtcgaaa tcgtcgccgc ccaacgcgct gttgccgccg gtggctttga cttcaaacag
     1321 tcctttggtc agttgcaata cggatacgtc gaatgtgccg ccccctaagt cgtacacgac
     1381 aaacgtgcct tccgaggcgt tgtccagccc gtaggcgatt gcggcagcgg tgggttcgtt
     1441 gagcaggcgc aatacgttca aacccgccag acgcgcggca tctttggtgg cttggcgttg
     1501 ggcatcatcg aaataggcag ggacggtaat caccacgccg accaaatcgc cgcccaaggt
     1561 ttcttcggcg cgcgatttaa gggttttgag gatttccgcc gacacttcga caggcgtttt
     1621 caccccctgc cgcgtatgca gttcgataac gcgttgattg tcgccgaaac ggtaaggcag
     1681 gtaatgcgta ttttgatgca gatcggcaag cgtccgcccg ataaggcgtt tggcggagct
     1741 gacggtgttc agcgggtcgg ttttttgggc ggacagggcg gttttgccga cttcaatgcc
     1801 gccgttttcc agatagcgga cgacggaagg cagggtaacg cgcccttcgg catcgggcag
     1861 acaggcggca ctgccgctgc ggacggtggc gaccaagctg ttggtcgtac ccaaatcgat
     1921 gcctgccgcc aaacggtgcc ggtgcggggc ggcggacata cccggttctg aaatctgcaa
     1981 aagagccata aattctgcct gtcggtgcgg tgtgtaaagg aaggaaagct gcattttaac
     2041 agaatttgcg ccgatagttg agcaaaccgc taaggattgg gtcgtgccgc cgaaattgcc
     2101 ctctaccgcc aaatgccgat aaaatccgcc tttcccgccc atcattagga atataaatcg
     2161 tgaaaggact cgactattgc cgccaaaaag cagaagaaag ccgctccagt tttttgtcgg
     2221 gcttccgttt cctgactcag gaaaaacggg atgcggtaac ggttttatat gctttttgcc
     2281 gcgaattgga cgatgtggtt gacgaatgtt ccaaccccga tgttgcacag gcgacattga
     2341 actggtggcg cggcgatttg gacaaggtat tcggcggcgc gatgccggaa caccccgtca
     2401 atcaggcctt gcggcaggtt aaggaaacct tcaagctgcc gaaatatgaa ctggaagcct
     2461 taatcgacgg gatgcagatg gatttggttc gggcgcgtta cggcagtttt gaagaattga
     2521 aactgtactg ccgccgtgtt gcaggtgtag tcggctgcct gattgcgcgg attttggggt
     2581 tttcagacgg caaaacgctg gaatacgccg acaagatggg acttgcgctg caactgacca
     2641 acatcatccg cgatgtcggc gaagacgcgc gcaggggacg gatttacctg ccgatggagg
     2701 aaatgcagcg gtttgatgtc cccgcaagcg tgattttgca atgcagcccg acgggcaatt
     2761 ttgccgaatt gatggcgttc caaatcaaac gcgcccgtga aacctaccgc gaagccgtat
     2821 cgctgctgcc ggctgccgat aaaaaagccc aaaaagtcgg actggttatg gcggcggttt
     2881 attacgagct attgaacgaa atcgaccggg acggcgcgca aaacgtcctc aaatacaaaa
     2941 tcgccctccc ttcgccgcgc aaaaaacgca ttgccctgaa aacctggtta ttcggattca
     3001 aaccgcgccc cggcacgccg gaacgggcat aaggcgcata ccgcccgccc ttcaggaaaa
     3061 cccaaccccc gccgccccga aaaacggggt ttcaaccatt ggaggaataa atgatgaaca
     3121 cgccgcatcc gcgcccgaaa atcgccgtca tcggcgcagg ctgggcagga ctgtccgccg
     3181 ccgtcacctt ggcgcggcac gccgacgtta ccctgtttga agccggccgg caggcgggcg
     3241 gcagggcgcg cacactggcc ggaaataccg acggtttcgg ttttttggac aacgggcagc
     3301 acattttgct cggcgcatac cggggcgtgt tgcgcctgat gaaaaccatc ggttcggatc
     3361 cccgtgccgc ctttttgcgc gtaccgctgc actggcatat gcacggcggt ttgcagttcc
     3421 gcgccctccc cctgcccgcg ccgctgcata ttttgggcgg cgtgctgctt gcccggcgtg
     3481 caccgactgc attcaaagcc aaactgcttg ccgatatgtc cgatttgcag aagtccgcac
     3541 gcctcggaca gcccgacacg acagttgcac aatggctgaa acagcggaac gtgccgcgtg
     3601 ccgccgtgat gcagttttgg cagccgctgg tgtggggtgc gctcaacacg cctttggaaa
     3661 ccgcaagcct gcgcgtgttg tgcaacgttt tgtccgacgg cgtgctgacg aaaaaatccg
     3721 gcagcgacta tctcctaccc aagcaggatt tgggcgcaat cgtcgccgaa cccgccttgg
     3781 cggagcttca acggctcggc gcggacatcc gcctcgaaac gcgcgtatgc cgtctgaacc
     3841 ccctcccgga cgggaaagtg ctcgtcaacg gcgaagcttt cgatgccgcc gtccccgcca
     3901 ccgcgcccta ccacgccgcc gcgctcctgc ccgaaggcac gcccgaacac gttcagacgg
     3961 catatcaaaa ccttcgctat cacgccatca ccaccgtcta tctgcgctac gccgaacccg
     4021 tccgcctgcc cgccccgctg accggccttg ccgacggcac ggtgcaatgg ctgctttgcc
     4081 ggggcaggct cggactgcct gaaaacgaag tgtccgccgt catcagcgtt tccgaccgcg
     4141 tcggcgcgtt tgcaaaccgg gcgtgggcgg acaaagccca cgccgacctc aaacgcatcc
     4201 ttccgcattt gggcgaaccc gaagccgtgc gcgtcatcac cgaaaaacgc gccacaaccg
     4261 cagccgatgc cccaccaccg gatttgtcgt ggctgcaccg gcaccgcatc ttccccgccg
     4321 gcgactacct ccacccatac taccccgcca cgctcgaagc cgccgtacaa tcaggtttcg
     4381 cttccgcgga agcctgcctg caaagcctga acgatgccgt ctgaaaacgc cggccggcat
     4441 caggacgctt tacaaggtgc ggcaaaacgc taaaataccc ccgaccgacc accaaaaagg
     4501 aaaaagtatg gcgacactga ccgacaaaac catcttagtt accggcgcat cgcaagggct
     4561 gggcgaacag gttgccaaag cctatgcggc agcaggcgcg actgtgattt tggttgcccg
     4621 tcatcagaaa aaactggaaa aagtgtatga cgcgattgtc gaagccggat accccgaacc
     4681 attcgccatc tgctttgacc ttattagcgc ggaagaaaaa gaatttgaac atttcgccgc
     4741 caccattgcc gaagccacgc aaggcaaact ggacggcatc gtccactgcg ccggctattt
     4801 ttacgccctc tcgccgctgg atttccaaac cgtcgccgaa tgggtcaacc aataccgcat
     4861 caacaccgtc gcacctatgg ggctgacccg cgccctgttc ccgctgctga agcagtcgcc
     4921 cgacgcgtcc gtcatcttcg tcggcgaaag ccacggcgaa acacccaaag cctactgggg
     4981 cggcttcggc gcgtccaaag ccgcgttgaa ctacctgtgc aaagtcgccg ccgacgaatg
     5041 ggaacgcttc ggcaacctgc gcgccaacgt cctcgtcccc ggccccatca attccccgca
     5101 acgcatcaaa tcccatccgg gcgaagccaa aagcgaacgc aaaagctacg gggacgtgct
     5161 gcccgcattt gtctggtggg caagtgccga aagcaaaggg cggagtggcg aaatcgttta
     5221 cctctaaatc cggaaaaggc aggcatcccg cccgcgccgc gattccgttt gaaaaatatc
     5281 cgtaccttcc gcgcgcatcc gtcccgcaca gacaaacacc ttgacttaaa tttacataag
     5341 gacaataatg ataactcgtt atttccttaa cttgacctta gggtaaattt tatgaaaacc
     5401 gtttccaccg ccgttgtcct tgccgccgct gccgtttcac tgcccggctg tgcgaccgaa
     5461 tcctcacgca gtctcgaggt agcaaaagtc gcctcctaca atacgcaata tcacggtgtt
     5521 cgtaccccga tttccgtcgg aacattcgac aaccgctcca gcttccaaaa aggcattttc
     5581 tccgacgggg aagaccgttt gggcagccag gcaaaaacca ttctggtaac gcacctgcaa
     5641 cagaccaacc gcttcaacgt actgaaccgc accaatttga acgcattaaa acaggaatcc
     5701 ggcatttccg gcaaagcgca taacctgaaa ggcgcagatt atgtcgttac cggcgatgta
     5761 accgaattcg gacgcagaga tgtcggcgat catcagctct tcggcatttt gggtcgcggc
     5821 aaatcgcaaa tcgcctatgc aaaagtggct ctgaatatcg tcaacgtcaa tacttccgaa
     5881 atcgtctatt ccgcacaggg cgcgggcgaa tacgcacttt ccaaccgtga aatcatcggt
     5941 ttcggcggca cttccggcta cgatgcgact ttgaacggca aagttttaga cttggcaatc
     6001 cgcgaagccg tcaacagcct ggttcaggct gttgacaacg gcgcatggca acccaaccgt
     6061 taaggagaaa tgatgaatcc gaaaaccttg agccgtttgt cgctgtgtgc ggcggtcttg
     6121 gctctgaccg cctgcggcga caacgggcaa aaatccctgt attattacgg cggctatccc
     6181 gataccgtct atgaaggttt gaaaaacgac gacacttcgt tgggcaagca gaccgaaaag
     6241 atggaaaaat actttgtgga agccggcaac aaaaaaatga atgccgcccc gggtgcgcac
     6301 gcccatctgg gactgctgct ttcccgttcg ggagacaaag agggcgcgtt ccgccagttt
     6361 gaagaagaga aaaggctgtt tcccgaatcg ggcgtattta tggacttcct gatgaaaacc
     6421 ggtaaaggag gcaagcgatg aaacctttga ttttagggct tgccgccgtg ttggcgctgt
     6481 ctgcctgcca agttcaaaaa gctcccgatt tcgactacac gtcattcaag gaaagcaaac
     6541 cggcttcaat tttggtggtt ccgccgctga acgaatcgcc cgatgtcaac ggaacatggg
     6601 gtgtactggc ttcgaccgcc gcgccgcttt ccgaagccgg ctattacgtc ttccccgccg
     6661 cagtcgtgga ggaaaccttc aaacaaaacg gcttgaccaa tgccgccgat attcacgccg
     6721 tccggccgga aaaactgcat cagattttcg gcaatgatgc ggttttgtac attacggtta
     6781 ccgaatacgg cacttcatat caaattttag acagcgtgac gaccgtatcc gccaaagcac
     6841 ggctggtcga ttcccgcaac ggaaaagagt tgtggtcggg ttcggccagc atccgcgaag
     6901 gcagcaacaa cagcaacagc ggcctgttgg gggctttggt cagcgcagtg gtcaatcaga
     6961 ttgccaacag cctgaccgac cgcggttatc aggtttccaa aaccgccgca tacaacctgc
     7021 tgtcgcccta ttctcacaac ggcatcttga aaggtccgag attcgtcgaa gagcagccca
     7081 aataagcata tgccgtctga acctctccga gcttcagacg gcatatgcga atcttgatac
     7141 ttgcaagccg caccgcccaa aatcgggcgg ccggtttcaa tatccgaaca aggttcgggt
     7201 tctgtatttt gcagaacccg aaccttttat tcaaatttaa acttaattcc ggcgtggttg
     7261 tatggtaaat taacacgcaa tatgccctga cctgcaaaac ttccccaaac ttcatcaaaa
     7321 aatcaactgt acccgtcttt ttcagacggc attgataccg taagaaattt cccatgcaga
     7381 aatggcaaac cgagctttat tccacaccgc tttggctttg tctgacactg aaaagcgcgg
     7441 tcaaagtctt gctgctgatt acggcgatga ttacgctggt gttaaccgaa atgcggctga
     7501 atgtgttgag tacctttatg tccaaagggc tttacgactc gatgcaggat ttgaaatgct
     7561 tcggcatttt ggatgtttgc tgcaatgaac gcgggcgtgg tgctgatacg ggcgtttaac
     7621 aacgtcgtca acgacttcct cgatcaaggc ttggcgatta aatggtcgga gcggctcaac
     7681 gaagtgctga cagcacgctg gcttgccgac aaaaactact accgcctgca aatgcgccgc
     7741 cacgcgccag acaacatcga ccagcgtatc caacaggacg cgcaggaatt tatcgcttcg
     7801 accatcgaat ttgtgcgcgg catggtcaat tcggtcgtta cttctctgga atttgccgtt
     7861 gttttgcggg gcttggctgg catcctgacc gtgttcggct tcgacattcc gcacggcatc
     7921 gtttggtttg tctatatatt tgtgattttg gcgaccttta tcgccatgtg gataggcaac
     7981 cctttgattc gttacaatta tgaaaacgaa aaactcaacg gcgactaccg ttattccctc
     8041 atccgcgtgc gcgaccacgc cgaaagcgtg gcgttttaca acggcgaaaa acatgaatac
     8101 gaccagcttt ccgaccgctt taaagccatt atccgcaacc gttggcgcat tgcgcggcaa
     8161 agcgtctgct taagcggctt taacgatatg ttcaccaacg gcatcaaact cttcccgatt
     8221 attttgcaag ctccgcgcct gtttgccgga caaatcaaaa tcggcgacat ccagcaaacc
     8281 gtccaagcct tcgcgcgact gcaaaacgcg ctttctttct ttcgaatgtt ctacaacaaa
     8341 ttcaccgcct accgcgcccg attggagcgt ctgtacggct ttttgctgag taccgaagaa
     8401 caacacagcg cgcggcaacc cgagattagc gaagtttcag acggcattgc actggaatgt
     8461 aaggcggcgg cagcttttgc acgaattggc ggcagctttt gcaccgccga agcgtaggaa
     8521 attgtattag cttttgcaca aatttatccc cgtttaatgg tctgttaaac ggggattatc
     8581 ttatgccttt aaaagtagta cgctacttgc ggtcagaact tataaatcag gtggtcgtcg
     8641 tagcttgaat tgctgttgat gtgctgctca cagtcaaggt ttggctgcct gcaaaacgct
     8701 cccatccggt caggcgcagt tctgccacga ggtcaagata agtaggcagt tcgctgcggg
     8761 tgccggagaa gaagacaaat ggcggccgca ccattgccat caggcgcaga aactctacca
     8821 tgccgaagta gttcatcgcg tccccttttc cccggcggcg cggctttggt tgaaccgcgc
     8881 gtcgtgctgc ctgcctttca gttcgctgat atacgcctgc cggcagtggt ggcggtcgaa
     8941 aaatacgccg ttgatcaggg tgtataaaac cttccagcgg gttttgggct tttgggcgag
     9001 ccgcgcgccg cgataggtgc ggcttgagag ggtttcgtcc gccgcgccgc cggttagggc
     9061 gttgaacagt tggtcgatgg ctaccaacag gtggtaaacg tattttttca gccgatgttt
     9121 agcgtccatt cttcgatttc cttttccagc gcgtccaatc ctggcgcggt ttcgatggcg
     9181 ttcaatttgt cttcgagctg ctgacgcttt ccgataatcg cgccggcggc aacagccagg
     9241 cgggcggatt tttcgataac tttttcaatc aaaacgtcca attccacgcc ccttgcggcg
     9301 gcgatttgcg ccagcatcgg ggtcggggcg ttgttgtccg cctgccgcgc gagggcttct
     9361 ttttcctgcc tgtaaaagct gtcgatttcc acttggggat agcccgccaa gaggctgttt
     9421 ttgagttcgt ccgcctttgc cgcgaggcgg aatgccgtgg cggttttttg ttcggcgaaa
     9481 cgggcggcgg cagcggcttc gccgatttcc catttcttgc cgtcccattc gtggtattcg
     9541 gacgggcgcg gcggggttaa aacggggcgg ccgtcggaat ctgcggcaat ctgcccgccc
     9601 tgcgcctgtc ctgccaaaag ggcggcgtat tcttcggcgc ggacggcaac cgcgccttcg
     9661 gggatgctgc ccaaggtgtc gtcgtaaaag ccgtttttga aatagatggt catttcgggg
     9721 tttccttttt tgtatttaca ttttggtttt ttgccgctta taatgtaacg acaaaatacg
     9781 ctgcaagctg caatgaagat tgaatttgac tccgaaaaaa atcaacgcaa tatcgaagag
     9841 cggaatttgc cctttgaatc agtcgggcaa atacgctgga caacggccgt tattgtgccc
     9901 gatgtctgct ttgattaccc cgaaccgcgt tatgttgccg ctgcttatct aggcgatacg
     9961 caacgcctgc acattgtctg ttttacgccg ataaaagacg gcatccgtgt catcagtttt
    10021 cgcaaagcga ataaaagaga ggttaaaaaa tatgcaacgt caatccttaa caaacgctga
    10081 cggcgaagtg cgggaacttg ccgccgaaga ttttgcgctt gcccgcccca ttgccgaagc
    10141 cctgcccgaa gacttggcac aggtgctttt ttcccatcaa aaacaattgg aagaaaaggg
    10201 cgtaatgcaa aaacgaaaca caggcaaaac cccgaaacaa ctcgttacca tacgcctgtc
    10261 cgccgatgtg gtcgaaaaat tccgcgccgg cggcaagggc tggcagacgc gcatcaacga
    10321 ggttttgcgc caatatgtcg cccaattgaa ataacggggg cggccgctgc ttatcgggcg
    10381 cgcccgacta tcctttcccg cgcggtttcg gccgtgcggt tttttgttgc cggtgccgtc
    10441 cgaagggggc agcggctttt tgcgcggtca ttcccgcctt tcgttattcc cgccactttc
    10501 cgtcattccc gcgaaggcgg gaatcccgaa acccctgccg ccgtcattcc cgcgcaggcg
    10561 ggaatccagt ccgttcggtt tcagccgttt ccgatagatt cctgccgcgt ttggggtctg
    10621 gattcccgcc tgcgcgggaa tgacggcggc gggggtttct gttttttccg atagattcct
    10681 gttgcgtcgg ggtctggatt cccgctttcg cgggaatgac ggcggcgggg gtttctgttt
    10741 tttccgatag attcctgccg cgtttggggt ctagattccc gctttcgcgg gaatgacggt
    10801 ggcgggggtt tctgtttttt ccgatagatt cctgccgcgt ttaggggctg gattcccgct
    10861 ttcgcgggaa tgacgaaagc ggcgggaatg acgaagagtg gcaggattgg cggggcatag
    10921 gtttcggggg attggcggcg cggccggttt cggtcggatt gcttggatcg gcgggctgaa
    10981 gcccatctct acagcccatc tctacagccc atctctacag cccatctcta cagcccgtct
    11041 ctacagccat ctctacagcc cgctagtctt gcagtgcctc gagcaagccg tctcggcggc
    11101 ggcggtttcg gggtccggtt gatgttcttc cactctctct ttgcggttct tcgttggttt
    11161 caggtatttc ggggcctatc ggggagctgg cggcgtttcc cgaggcggct ttgccgatgg
    11221 ccatccagtc gcaactgcct tcgtaccagt tggtaataat ggcggcggcg tggttttttg
    11281 cgcggataaa cagccagttg tgttgccttc gatcgccggt aacgcgtccc gaatggcgtt
    11341 cggatacgaa gcatttgacg ttgccgtcgg caaaggcgac ggggaagacg acttcgtttt
    11401 cgatggggcc gccgcttctt gtaaacctgt aactgccggt ctgtatcatt gtgccgtcgg
    11461 ggaatctgac aatatccacg ccgccgatgg tttggcgtgt gaattgcgcg gcaacggcgg
    11521 tgctgatttt ttcgtccagc ccttggattt gggcggcggt gtgggtgtgg tcgcggtcgg
    11581 ctttgtcttg caaaccttgc gccagcgtgc ccgcgtccag cgcgcctgcg ttgatggttt
    11641 cgccgtggga ctttatccaa aacacggctt cgcccaaggt gtcggcggct ttgatgcaca
    11701 gttttaaaac cagggctttg gggcggtttt cgtcgccgcc cgttgccatt ttgctgtccg
    11761 agcgcggggt taaaaagccg ttgtcgttta aatcgccgtc cgtccaagtc gatacgagcg
    11821 cgcttctctg cctttcgttg tggtcttcgt aaccgacggc tgccgtgtct gtgtggtttg
    11881 tccagtgtga aaatactttg tggacgtgcc gtttgatttc gtcttcctgc ttcgttccga
    11941 ctgccaagct gttgcctgcg ttgcggataa agcggtcttc cgcctgcggg acgttttgga
    12001 tgctgccgta ttttccggtc agcagacggt acagctcggg ataagcggtt tcggttacgc
    12061 gcgtgcggat gtcgtcaaac gccagccagc cggtcgggat ttggtcggac ggaaaccacg
    12121 cggtgatgcc gatgtcggta cggctcaaat cgggcaggcg gttgctgttg cccaaggcgc
    12181 ggtagaggtc ggggaaggtg ttttgcgcaa aggtcgtgcc gttggctttg agatagcctg
    12241 cggggttttg tacggctttg ggaaaggata cgatcgcgcc ggcggggatg ccgtccgaat
    12301 tgagtttttt ccatgcggac cagttgtcgt ttgcgctgct cgtttggtgg cgttcgtaca
    12361 cgtcggacgt gtaggcggga taacccaact gcctgcacca gccgggttgc gttccggcga
    12421 tgacttggat atggcaggcg gtgttttcaa cgggcaggtt ggagctgccg accgccgtcg
    12481 gcagggaata gatgccgtct gttttgaggg tgttcaaatc gccccggaat gtttcgactt
    12541 tgaaattgcc gatgccgtat ccggacagtg tgttcggttt gcctttgatg tcttcggcaa
    12601 aacgcggttt gttggctttg tggaagatgt cgtggttgtt gtaggaaatg cggttgtttg
    12661 cgccgtgcag gcggagggtg ttgtcgccga agacgatgtg ggcgttgtcg gcagccgcgc
    12721 cgatgtaggc tttgtggccg ccgttccagt tcgtctggtt ggcggataaa tggatgccgc
    12781 tttggaattg ggtttgggca gtaaaggttt tttggccggt gatggtttgg tcgccggtgg
    12841 taactacagc cagcctattt agtgctgcca gtaattgatt aggctgcgac ttatccggct
    12901 ggataccggc cgcagtcagc acggccagca gctcggattg cacttggttg agccaccacg
    12961 cgggcaggat ggtgcccaat tcggacacgc cgtcgccgtc gtggaaggtc ttgtcggggg
    13021 tttcgatggg gtgcatggtt tagtccttat cggtaggtaa atcggatagc ggtgtgggcg
    13081 ggcttgaggc ggttgaacag gctctcgatc acggcatcgc tgtaatcggt cagcctgtcg
    13141 cccgccgccg agataccggc gcggaatcgg gtaatgcggt tgttgccgcc gcgcacgtta
    13201 acgtgccaca cccacatgat ttcctgcggc gcaagacggt cgccggcgcg gtttacaccg
    13261 gcgcggaacg gctgcggttc gtcgatttgg atttgatagc ccgccgcctc ggccaaacgc
    13321 acaaaataag gaatactcaa gccgcctgtt tcgtttagct tggccatgac ggccaacaca
    13381 cggcgctggc ggtttttgcc cgtaccgtcc aaacctaata cgcgctccca atcggccagc
    13441 atttggccgg cgctgctcgg gtcgacggca tcggcaacgc tttgagccga ttccgccacc
    13501 gcatccagcg ctgcgccgtc tatttctgcc tgcgcccgca cacgcggggc attgcgggca
    13561 tacgacacgg ggggcaacag accccgcaag atgtcttgat agctcatgac ggcatctccg
    13621 ttacattaat cgcgcccgct ttaaaccact cgatgcggtt aaccgtatcg gcggcacggt
    13681 tggccgtcgg cgcagtcaga cggcggtcga tcacaccatc cacattgctg atagccgcct
    13741 cgatttgcga cacagtcaga ccgtcgccgg ggatcagggt gtcgaaatat tcttttagag
    13801 ccgtccgtat gcggcgcttg gcatcgtcca agtcgatgcc gtcgagcttg acttgcacgg
    13861 taacaggcac cgccgttacg gttggcttga gtaccagcgc attttttgcc gttaccgggc
    13921 gcatctcgtc gatataagcc tgtacgcggc gcacagtttc ttccgacggc acaccgtcgg
    13981 cggaggtaat ggcaatatcc accgtaccca agccgcggcg cagcggataa acatatgcgc
    14041 tggttacgcc gtcaacactc aacgcccagt ttttatagtc gtaacggttg ccgcctgcgg
    14101 gcggtcggcg gatgatttcc aacagacgcg ccagcagtga ggcatcgctt tctcggtcgg
    14161 tgccgccttg tacggtaagg cggcattcgg tggacacacc ggcgggggcg gccatcagtt
    14221 gcgcctcgcc gtcgcgcaca ttggcggccg cgcccagctc gtcggcgatt gccggtattt
    14281 ccgccgtgcc gccgctgccg ataacggcgc gggcggtagt gcggtaaaaa cgctggccga
    14341 tacgcacttg caggccgtct gaaagcatgg attgcgcaat accgcttacg gtcagcgtgc
    14401 cgctggccgt ggtaggattg cggcggcgca agccgcgcat ggaggcatgc cgctccaaat
    14461 actcgcggtc ggcggtatca gggaaaatct gccgcacaat ccagctttga tgcgcatatt
    14521 gcccttcggc gcagctggcc aaacggctgg cgtgcacata atggtcgctg tcggggctga
    14581 tatcggcatc cggccacagg cttttggtat cgcgcaggat acgctcgcgg atttgctcaa
    14641 atgtcggcgt ttcaaacacg tttaaaacct ctttaaatca cgggcacttc gtggcggtaa
    14701 tcaaacccgc ccgccgccgt atccacccgg atatgcagga tcaggcggcc gtcgtgcggc
    14761 tgctctgcgc gcacggtaat cttgtcggca cgcccgctct taacaatcgg ttgcagtgcc
    14821 tcatcggcat attgctcggc caacaggctg acccgttgca ggtctttttc gcgctgcaac
    14881 aaatgcagca gcgagccgag cgttttatcc gcccaccagc tgcccaacgg tgtcatcaaa
    14941 cggatataca cggcattttg cagcgtatcg acggtgcggc cggtatagtc gccggtgccg
    15001 gggtttagct ctttgtccat gttggcattt tgccgttttc agacggcctc acggagtaca
    15061 cgggcttcaa tcccgcacgc acaaaaaagc agcccgaagg ctgccaaagg tctgcttgtc
    15121 tatgccggtt ccgccggtaa ggttttgccg ccgatgctgt cggtatgcgg gtgctggcgc
    15181 aacgatatat tgccggccac cacgtcgccg tcggtgttaa agctgccgcc cgtttggtta
    15241 acatcgccgc taaaggtggc tccgtcgccg ccctcgacgg ccatgccgcc gttgccgttg
    15301 atttggcctt gcgccgtcaa cactgcactg gtctccacca acggagcgtt aaatttggca
    15361 tccgtggccg cattaacctc gtattgtttg cagttaaccc ggtacacgtc gcaatcggcc
    15421 tcaatgattt tgccttgctt aatcacgatt tttgcaccct catgattaaa aatcgccgtc
    15481 tcgccgggct taaggttttt gatgcggtag ctgccgtgct ggctgcacac aatcacaccg
    15541 tgcgaagtat tgccgcccag cggtatcact accgcttcgc tgccgtcggg cggatggctg
    15601 gcaaagccgt attcctgcaa atgttcaaag tcttgcaggg tttcgtcggc caagccgctc
    15661 aactgcacgc gctgtatcgg ctcggacgac accaccagcg tgatttttcc ccgaaaggcc
    15721 gcgcgcaggg tttcgccgat atttttagca gtttgtgccg tttttttcgc caatttactc
    15781 aaactcattc aaacaccgcc gtttctgctt gttttttgcc gccttttttg cccttatggc
    15841 tcacgccttt gcgtttgcct ttgcgcttgc gcgccgcctc ggcctttttg gggtaagcgt
    15901 cgggtgtcca aataccgtcc tctttgagcc gcagctcggt ttgcgtgcca tccatgcggg
    15961 atagcataaa ccgccgcccc atcagaaaaa acaccgcatc gataccgtgc tcgtcgtcga
    16021 tcacatgcac acgctggcca ggttgccaca atacgccgtc gcgggtttta tggccgccca
    16081 cggttatcgt gagtgtaaat ccctccagcc gccagtcggc cagctgcttt ttagcctgct
    16141 tttgcaatgc ggccaaattg tcggcatcgg acaccaccac cgttttaggg cggtgcagcg
    16201 tcatcgtcgg gtctttgtac acccacttta aatcgtgttt ggcgctgtcg ccgctgcggc
    16261 cgtgcgattg cgccaaaaaa gtaacctcgg aaaagcggtt gtcggtatcc cactcaatgt
    16321 ccatgcgctc gatattgcag cggctgtcgg tgcggctcca acacaatgtc gccaccggcg
    16381 ggctgctgta atccgcaccg cccaccacca acgtgccgtc cggctccagc cacggatgca
    16441 gcccgaccga gttggcaata tgggttaatg cctgccatac ggtttcgccc ggctcgatgt
    16501 cgattttgtc caaagcgggg ttgttttcgg ccttaagcac caccgcttta atctgcggcc
    16561 acggcgcggc cagctttttg gctgcatcca ataccgtcat gccctttaca ttgagctgcg
    16621 gcgcggagca atccaccaaa aaaccggcca aatcacgccc gctcaagctc aactcgcggc
    16681 tgcccttgct tttgccgtgg cgctggctgc cgatgatgcc cgtcatcacg atttgcccgt
    16741 ctatcactac ctcgcagctc tctccgctta aatcgggtat ggccgcctcc ggccccaacc
    16801 tgccgatgac aaaatcgaag ctgtcggcag ggattaaaaa gtcgctgtcg atgtcgtagc
    16861 gctcccagtg gcggtgctct ttaccgccca cgcgcaccga cacggcatag ccgtatgaat
    16921 tattttgcat agctgttgac caaagtgccg cgcttgataa acgcggggtg gtggatatgg
    16981 ggattgagcc gcaccagctc tgctgcgcgg gccatatcgc cgtaaaactc gtgggcgatt
    17041 tggtgtatcg taccgtcgat tggggcttgg cgcacaatca gcggcggctt ttggttgatg
    17101 gccgccgcaa ccaacgcatt cagacggcct gccgccgcgc gcagggattc tgccgtttgg
    17161 taagcctcgg tatacacggc gttggccgtc agcccaccag actcggcagc agccgtctgc
    17221 accgcccgca aggcggcaat ctcggcctgc atacggcggc gcatggcgcg gttaacctca
    17281 atcaaatcgg gcgcggtcat ctcttcgcca tgcgcctcga ttaatgccgt tgccaccgac
    17341 aacagcgatg acgtggataa caggcgcacc gcctgcgcta ccggctgcac ctgtttggcg
    17401 gttaaccggt tcaggcggtt ttgcaggccg tctgaaaagc ggccggtcag taaattatca
    17461 gggatagcgg cggcacggtc ggcaacagcc acagccccgt caaaccgctg tcgcggcgac
    17521 caaccggcat ggtgcatggc attatcggcc aaacccgcct cacggcgtat gccagtatct
    17581 accatgaccg atatatccgc aaacagcttg gccgagccgt ttttaaacgc cgctgcgctg
    17641 tatccgcccc gatcgggaaa ggcaattttg tccaaatcaa acaaacggcg caccgcacca
    17701 aacgtacccg atgccgcact ccaaatgccc agcgcgctgc cccataaagc cgatacgccc
    17761 gcatccaccg ccaacaccgc atcaacaaag ccgatagccg cctcgcggta ggtgtcgata
    17821 tcagcaatca acgcctcaag ctcgaccaaa aaggcgtttt caaaaacaaa gatttcctgc
    17881 gcttcggccg cctcgcggaa agtaatatcg atgcccgcat aatccacata atcggcctca
    17941 tgtcggtaac tccatgatgc cgcaatcatg ttgtgcatcc gcccccaaac agggtgcacc
    18001 agcacggcgc cgcccggctg ctccagcgca tccagcagct ttttcagacg gcctgcatag
    18061 cccttgcccc aaaacaccgc attaatctgc acctgccgcc cggtcatgcc catgtcttca
    18121 aggtcgatac cctgcacaaa cggccgcgca tgctcggcca atgccttgcc gttgctctcg
    18181 tccaccacct caatatcaaa gccgacgccc ttgtaagatg cgtcctgcaa taaggtatgc
    18241 catccgctca ttattgaccc gctccacggc cgaacatggc cacttgatac cgcgatacct
    18301 cattcgcgat aacacgtccg tcgatattca ggctcacatt ggtattgatg gtctgacttg
    18361 ccgacgccat cgcactttgc acttgtgcca aacctgttgt aactgcagcc gtatcctgcg
    18421 ccaatgctgc ctgatacgcc gccgtctgct gctgaatggc tgcctgatac gccgccgtct
    18481 gagttgaaaa caacggagac aacatcttat cggtaggctg cgctgacgga gcagccaaat
    18541 actgagggac accaccccgc gcctgttccc gcttcggcgc aggttcaaat cggataccgt
    18601 tgtttttcag acgattaatc atttcaggag ttgtcttggt accacgcaaa ccctttggca
    18661 atgtgccgtc acccaaagac tcggaataca gcaacatacc taacaaaccc gcccgtttaa
    18721 ctaacgctgg attattcatg agcccgctac cggcagattt tccccatgac agcaacttac
    18781 cgcctgccgc tgtggcacct gctgccacgc cgcctgcgga agccttaccc catcccaacg
    18841 ctttactacc tacatctttc agaaagcctg cacctttacc gccaccggcg gttaataaca
    18901 ttgcggcaga cgccgctgtt gccgccgtcg tcaatgcttg cagggctaga gtggcattag
    18961 ggtattccgc tgccagcttt ttaaacgagg tttccatatc caccaatgtt cccgtcagac
    19021 tttcccgcaa cattgccagc gattcctgtt gctcttgttg cgccgctgcc gacaacattc
    19081 gcgcctcgtt gttcttagca atttttccgt tatccaacgt tacgccagcc aacgaagcca
    19141 tatattcacg gatttgcgtc atatccgttg cagccagcaa accttgtttt gcctgcaaat
    19201 caggcagcag ttgcgccaaa agcgcgccct taagcatatt tgcctgctcc gccgccgtct
    19261 tatcgcctgc agccgcgcgt ttcttataat cttggtattg cttatccttt actagcatgg
    19321 catcggcaag acgggacaac acctgcactg cgttttcgcc gttttgcttg ccttgcacaa
    19381 ccgagcctat ccaatcgaca cctttcttcg ggtcattcgg atttgccatc ttcttcagac
    19441 gacctatcgt gtcaggcgac agagttttac tcaaaagatt ttgcacatta gtcgccgctt
    19501 cggcaggact gcccgattta ttcgccgcag attgtaagag tgagagcagg tagtccaaac
    19561 cgccgacacc attcatccct gcctgttgcg cggcagggag caggctcggc agctcccgaa
    19621 ccatatcccg cacctcgaaa gtgccgtcta aacccgattg caagacgtgc tcaagcccga
    19681 gttgcaggtc tttaccgctc atgccgccat ctttcagggt tttaatcagt tttgccgtat
    19741 cctcgccact gccttctgag gcaagtgcaa aagcatatgc cgcctgcgct tcattcttgg
    19801 tttgggcaaa attcagactg gtggtcatca tgccgctgat taaatccaaa gccttatcgt
    19861 gggtcccgcc atttttctcg acaagttcaa gtgccaaatc cttgatctgt tgcgcacctt
    19921 cagttgcaat ccacgctgcc gatttactgt tatcctcaat aaatgcctgt ctggacacgc
    19981 ggttgatgtt ctcatcaagc tgctttctgt tgtccatagc aggcttaagc accgtatacg
    20041 ctgccgcgcc accagccacc gcagctgcac cgaatcttcc gatttttccc atcttgtccg
    20101 caaatcccgt gccctgtttc agttccgcgt tcagctcgcg aattcggtta cgcgtagcga
    20161 ccgccgcccg tgccaaatca ttttgtgatg ccttgccgct gcgcgccaaa cggttaaacg
    20221 cagcctgtgt cagcatgatt tcgcgttgaa tctgtttttc tgagcggatg ccggtcaacg
    20281 cataagtatg gctttgccgt tcaagttgcg cccgcgattt cgcggtacga tctaattgtc
    20341 gttgagtatc agccagtagc cgtctcgctc cgtcatctcg ggcggttaac accaacgaca
    20401 gtttcatgtt cccgtttgcc ataaaaagca gcctttaaac gtattaactc aaggtcaatt
    20461 atcgtttaaa ggctgtttaa gggaggcggt atgggcttca accccgtttt cgtgctttat
    20521 ttgtttgttg cgttataata ccagaatgat aaccatttta ttcaaaggga tattttatga
    20581 atatgaaaaa attgatttcc gcaatttgtg tttcaattgt tttatcagcc tgcaaccaac
    20641 aatcaaaaac ggcacaagcc gaagaacctg tccaaagtat ccaggctgct gattgtaccg
    20701 ccccaatgga catcacagtt gaacaatatc tcatcaattt ggagcaagca tttaaaactc
    20761 agaacgtctc aacaaaaatc cataataaaa atattgtcaa gaccgattgt ggttatgacc
    20821 ttactttggt aatggatttt ggggcgattg cgctcaaact ggacgagcag caaaaaatta
    20881 gagctatctc agtaggctac attttaaaaa ccgacggaga gaaaggacaa aatctagtca
    20941 ataatgccat aaatggatta cacagtattc aggcagttct gtctttaact accacagaca
    21001 aattgggcga atcggaagca ggaaaacaac ttcgtcaaag aatccaatca gacaggagca
    21061 acagcgcaaa aagacgttcc ggcagatggt attttatata gcgttgtttt tgaaaaagaa
    21121 acaaacacca ttgcaataat cggcagaaaa caaccctaac cgtcaaataa aaaaaaggtc
    21181 gtctgaaact cagacgacct ttttattttc cctccggctt tctcagccgt ttcgacacaa
    21241 tcactccctc ttcctttggc gtttttatgc ccaggctcga caggatgtct tcaaaccatg
    21301 ccgacagctc ggcgtggcac atatcgcgca ctgcgtccgc ccctatcccc gcctttgcca
    21361 tcaggatgac cgcctgacgg tagcttttgt gtgccgtcag gtagctgcgt tttactgctc
    21421 cgccgccgac ggcgcgtcgg ggctcgccgt agcggcggac ggttttaggc acaaatcctc
    21481 ctgagccgcc atcacggtat ggtagtcttc ggtagagagg ttttccatca gccactgcgc
    21541 cgtcaggatg tcggatggga taccttgggc ttcaatctgc tgcgaccagt aggtcagcgt
    21601 ctctaggacg tcgcgttgca gcagttcggc ttttgtggcg ttttcgggca gctcgggcaa
    21661 gtcatcaatc agggcgaacg ccgccagttc gccgccgacg gtcagtggct tgaggcgtaa
    21721 ttcagatacg actttgccat tgtaggtcag cccgtgggtc agtttgacgg taatcacgca
    21781 ttactccttg attttgtgca aagcaatcat ctcaatgtcg atgcgtgcct cgttatcgac
    21841 ttcatattgc tcgctggtat cgacggtaaa gcagtcgagg taggaagtgc ggcgaccttc
    21901 gtcgttgatg gggtaaatcg tgattttcgc cttggtgatg ttgtcccagt cgatttccgt
    21961 accgtcgatc ggaatggcgg cggtaacgct taatttgtgt tcggttacgc cgtcacaata
    22021 accgttgact cggccgttgc ggttcatcgt tttgaccggc ttgcggcctg tagtggtttg
    22081 cggcttgatg ctcacaatct cgatatcgcg gccgttcacc tccataatca ccgcgccggt
    22141 ataggtagcg tcgctcatgc tttatccttt tttaaggttt cagacggcct ttaaacctgt
    22201 tttaaacgcc gtctgaaaag ggttacaaaa tcaaatcaat gcgcccggca aagacgtgca
    22261 ggccgttgac cacatcggcg gggataatgg cattaacacg gttggggtcg ttttgcgcac
    22321 gcgccaccac cagcttgcct ttgttggcct cggcgttttc gatgatttcg gcttggtcga
    22381 gcttaatcag cacgtccaaa atctcgctct taaccttggg cagcaggcgg tcgctcaatt
    22441 tgtcgcgcgg aaaacgcagg gcaatgcgct ctttaacgct gcggcgcaca taatccagcg
    22501 tgcgaatggt ggtaatgtcg agtagtgccg ggtcgtcggt gttgttggcc gacttggtat
    22561 aggttgatac ggcacgcata atctgcacgc ggttgttgac cgctgtgagc ggggtcaagc
    22621 cgttgtacag cgcattgttg cattctgcaa accacggcca ttgcgcatca ggtgtaacgg
    22681 ccagcccttt gatttccagc gtgttcagcg gcttggcagg gtcttcttca aaggccaaca
    22741 ccgccgcata acctgcggcg atgatgccgt ttggctctac cgcacctttg taccaagcac
    22801 aggtaatgcg gccgtcgttg attttgccgg tagcggtggt ggccgtgctc aaggccgcac
    22861 tcatacccaa tacgccgata cagccgcgct gctcgatggc gttgaatacg ttggtaatat
    22921 ggttgctcaa ggctttggcg ttggcgtcat cgctaaacgg gctgcaaata atgtgataat
    22981 gcttgcccgc cactttggac aaggccgtgg caatgtccgc atttttggca ccgccggtaa
    23041 agacattggc ttgataagtc aggccggtat tgccggtgct cacggttaag ccgctctcgt
    23101 tgccgatctc gcctttgtgt ttggccgtca gcgtaacggc tgcgccgctg ccggatgcgg
    23161 taacggttac atcggcggca gtgatggcgg ttttcagacg gcctgccact gtggcggcgg
    23221 tctcgccggt gttaacggcc gtgcttacct gcttgccgcc aatcgtgatt tccaccacgc
    23281 ccggcgcggt ggccgtgccg gaaagggtaa cggttgcggt tgcctgcacg cctgcgctgt
    23341 ggtcggcaat accgataacg gtcaaatcca aataagggtt gttggcaaat gcttggcgca
    23401 ccatcaaatg cgccagcgag ccttgtccga acaaatcggc cgcctcggca tcgctaaata
    23461 gttgcaccgg ctctaaggcg ggctgtatgc ccgcggtcag catgggtgca accatcaata
    23521 ccttttgcgg attttgcggc aaaccttgca cggcattgcg ggtgttaaat tcaatatact
    23581 gcccgggcac gcggatgctg cccggaatcg tgtcaaaatc aatatggggc atcattactc
    23641 tcccttagta ttgcgggttt tggtatttgg ggcggcatcc tcaaccacca ccaaatcgcc
    23701 gtcatcaatc atgcggcggt aatacaggct gttgccgtcc acctccaccg gctcttggcc
    23761 gatatattcg tgcgggttgt gttcggtcgg cacacgcagg ccatctgccg ccgttacttt
    23821 gattttgctc attgcttatc cttaagggtt aggtttacag gtatttcatc ggcggattgc
    23881 gggtcgtaaa ttttgccgtc caacccctcg aaatcaggcc acggctcgct caatgtaccc
    23941 tgatacttgg taaagatatg gttggggtcg tcgggattgt cggtgcgttc ggggtagcgg
    24001 tcattttcca acccgcaggt gttaaagcgg atggcatact caaccgcata tatgcttact
    24061 gcggcgtttt gcaccagcac atgattggca atcgcgcgca ccgctttggg caccaagccg
    24121 cggctatcgg caaaaccgag ccgctggccg tcaagcaggc ggcgaacagc gcggattaaa
    24181 tcgttgctgc cgatttcgcg gctgtcgatg ccgccttgcc gctgcgcctg ctcgttgcgc
    24241 agattgcggg ccgccaccat caccacaaat tcggcggtat cctgataacg tccgcatacg
    24301 ccgccggtgc tggcaggctc aactttgctg ccgccatacg ttacccaaac cgcaggcagc
    24361 gtatggattt gccccgccaa atcgtcggcc tcgccgttgt agcttttaac cgtgcgcacc
    24421 atgcgaccca agccccgttt cagacggtct gttatcgctt gctcaatatc agtgatcacg
    24481 cccgaacacc ttatttttac cgtttacaaa cacaataccg tcttcgcccg cggccacttc
    24541 ttcgccgcta tcatccacgc ccagctgcac ttcgcctttg gccatagcac gcagcaggtc
    24601 gagcacgtcg attttgtagc ggttgcggat ttcgtcggta atcaacacgc cctgagccgc
    24661 cgtcagacgg tagcgggcaa tgtcgcagca aaggcgcacc aagatgggcg gcagatcctc
    24721 aaaaggtcgt ctgaaccgcc ccagatacgc gtcgatttcg gcagtggcgt ccaccagcgc
    24781 ggtttgtgcg acctcgcggt caatcagccc ctcgttgttg cggtcggtga gctgcaagac
    24841 ttccagctca ccgaaacgcg caaccatatc ctcaaccgtc gcgtatgcca ttactcgacc
    24901 gccttgcgtt gcagcatagg ctcggcgcag attgccttcc acaccgcttc gccgacttcg
    24961 gcgcgcttca cttcacgcca gccgccgtca aacagcaggc cgccgcgcca aaattctttg
    25021 ccgtctgcgc cggtactgac gagcatcaca tcgcggctgt ccgccaaagc gtcggcggcg
    25081 cgttgcgtat gctgcacttt gagttcggca agttcggcgg acagtgcctt tttgtcgtct
    25141 tcggcttttt ccaaggctgt ggtcagcatt tcgacatcgt ttcgggcggc ggcaagctct
    25201 gcctgcacgg cgtccaattc ggctttgatg tcttcaaacg acggggcggc ggtttcggcg
    25261 gtttctggtt tgttgttggt ttttgccatg atgactcctt gtttcagacg gcctttaaat
    25321 aggtgttaaa ggtcgtctga aaacggttcg gggttacagg tggacgcaaa cgtgcagttt
    25381 cagacggcct ttgaaggtat tggtcgtgcc gttgattttg tcggcttcca ataattcacg
    25441 ggctttgtct tccagctcgg gcggaacgac caagaggctg ggcttcacat tcaacacata
    25501 gccaccgtcg gctttgattt tctgcatttt gacgataatg gcggcgaggt tttcgcgggt
    25561 cagcgcggtt ttttccgcca tgtgcatcag ttgccacaaa ccgaaaccgg cattgcagcg
    25621 gcggcgcgat ccgtacaggt attcgtcttc cataaacacg cggtcggatt tggacgggtc
    25681 gaatttggtt tcaaactcgg agtcggtgcg gttttggaaa atcagcggct tcagggtctt
    25741 ggtgtcatcg acgacgtaga aggtcggcgc gtcgttgtcc gtaccggtcg tcagattgga
    25801 gtgcggcgta ttctgacccg tgccgtccga tttctcgtaa acggggtggt cggtatcgaa
    25861 gaaatattgg ccgtcataac cgacagtggt tttacctttt ttgagcagac cccacaccag
    25921 cgtatcgggc agggcggcgg cggactcgcc cattgccgcc atcatcgggc ggtacatgcc
    25981 gacttggtcg tcttcgatgt ccgtacgcgc tacgccgacg gttgcttcaa actttttgtt
    26041 gtcgaggctc atcgcctgct tgttcatttt ttcgatttgg cgcgagccga cccattcgcg
    26101 catttggggg aatttgccga gccacgcata agtattggtt gccgtattgg acggaatggt
    26161 catcgcaacg gcggtatagt cgggcttgac ggattccaaa cccgtttgaa attctttgcg
    26221 gaatgctgcc gtgattgcgg tcaaaatcgc tgatttgtcc attacttacc ttcgctttct
    26281 ttgattttta caaattcttc gccggacatg cccagcattt ttgctgcggc tgcctcttct
    26341 gcagtcagtg cggcgacgcg ttcgtcgggt gctttaccgc ccgtttgcga gcctgccagt
    26401 gcagcgaccg gctgggcgtt ttcaataaag ccggtcaaaa atgccaagcc gcccggctgt
    26461 ttcaatacgc cttctgccca ctccttctga gcaggcagca atttgcctga agtcagcgcg
    26521 gcggtaatca attcgttgcc tttgtctgct tcctgcttgg cagtcagcgc ggcgactttg
    26581 ctttgcagct cttgaaccac tgagataggc gcgtatttag tcaaatcagg cttggcggtt
    26641 tgcgccgtca atgccgcgat gcggctgtct ttttccgcca gctgcgcgaa cacgtcggca
    26701 gacaatgcca cgtctttcgg cttggcttcc acgagcgcgg acaatgccgc cttcagttct
    26761 tcttcgcccg catcaggcag accgaacagt tgctgtaaca actctttcat agggttttgc
    26821 tccgtttccg gttttaaaat ttgcgccgac gctgccgcca gcacctcgtc cataccgtcc
    26881 aacgcgggga aatttgtcag cgcggcgtga aaaattttgc ttacatatcc ctttgtgtca
    26941 taggaaaaca cagcagagat gtagcgatac tcttttgcgg caattgccgc agccgccttg
    27001 tccgtccact ccacttcggc aaacatgcct ttaggcgtga actccagcca acgcatccaa
    27061 ccggcggcag gtgcaggttg tccgtttttc tctttgtaga gcgtctggtg ttcataatcg
    27121 acaaccaact gattgcgcga gctgttggcc aacaacgcga catcatgacc gttttcttcg
    27181 gtcagatacc acgcagggac atcagtcgga cgaccgtcta ctgcgcgaaa ttcgccatat
    27241 ggcagcagtt ggattcgccc gtcttttggc tgcacctcga aactgcacac ggcaagtagg
    27301 gttttttgtg catttttgga catgtgtacc tctctcaata cggtcatttt gcctatttga
    27361 aaaaacaaca aagagtaggc gtgcttcaaa ccctcccgcc atttttttaa aacctgtccg
    27421 cgcgatacac aattaatcgt tttcaggggc tttttagaag cgtctaggat tgatttttat
    27481 atcttgggca ggggtttgta ttacccgcat aaaaaaacgc gctaaaaagc gcgtttttga
    27541 gggttttata aattcacggt atcagacccg aaaaataatc ctgcacatcg tccatcaaag
    27601 cctgtttgtc gtcgtccgtc agcgtcaaaa attcccgttg cggaatccga actttgcggt
    27661 tgcgccccgc cataccgccg aagttgtgga tggcggcata gacgatattc gtaccgacaa
    27721 gggcggtatc gttgtctgac agtgtggaaa aactgtcttt cagacgaccc gaatccgaaa
    27781 gcggcttgcc gtcgcggtat tttagcccca accatttcgg acggcctgcg tagcggaaat
    27841 tgagcttgac cgccgtgtgc atggtttcgg acagtcggcg catcagcagg tagcggtttt
    27901 cgatcccgtt gccaagccgc tcgatttggt ttaggacgac aaagatattg tctattttga
    27961 catcaatcat ggtattatcc tgcctaggct actaaaaaag cggtgaattt gtctgccctc
    28021 cgcagccctt atgcagtcct cggactggga ttggtatccg ttacataagc gagagtgtgt
    28081 cgggattgca gtccgactta gtagcctttt ttcatttcat agcgtttctt acttttcaca
    28141 atttcttttt catccgccct gcgaaacgaa tccaaaaaaa tcgaatcgta ctgtggcaga
    28201 tatttcaaaa ctgccatcaa tctgtatttt tcgttcccct ccaacggttt tccgagcaaa
    28261 acaaaacttt cttttcctct gcttttgtca taaaaaatat attcgggctc atgaatcaga
    28321 tcggggagca tggcatataa atctgttcca aacgattgcc cctcccgact gtccacctgt
    28381 ttgaccaatg tatcatcaga cagccacacc gtcgctcgtg tcatacctgc caattcttgc
    28441 gtttccttgc tcaatacacc cgcagcaaat ttaagctgtc ttgatagcgc atttcggatt
    28501 ttgattttct gctctttatc gggcttgcca tcaatatcca aacgttgctt gacttcataa
    28561 aactcttttt caagctgttt aaagctggtt ttaaaatccg caccacccat ttccgctttg
    28621 gcaaattgat gcgccaacgc acggtcgtac ttgtctaaat cggggcggta gttcattcgt
    28681 ccggcgttgt aatcaaatcc tcgatcggtc gtgtacagac tgccatccgg tgctttatag
    28741 gcaagggtca gataagtatc gccttttttg ttgtagattt tatgggtctc gactagattg
    28801 tccgccgtgc tttgcccgac aatccgcccc tggcgttcca catcccgctc cgacagcgcg
    28861 atgaccgagc aacggcagtt gtagccgttg ggcgggtaaa acgttgccca aaacgggtcg
    28921 tcgtagcggt acaccagccc gtctattgcc gaatgcgccg gacgggtgcg gctgtcgcct
    28981 accgcgtcat acatccaata aggccgtgca tcaatatttg ccatatatcc ttgatattga
    29041 ccggcgttgt aggcagtttg catgttggta cggtaaatcg tctccaacct ccgcggcgaa
    29101 ccgaatacct cgccggtggc tgggtcgatg atatccttac cgttatgccc gttcggatgc
    29161 agccagccct tgttgccgag cagattcaag ataccttttc gccaatcgtc aaacgactgt
    29221 ccactttttg ccgattcaac catcgaagtt ttgatgtcgt tgagcatatc caagtcggtc
    29281 atgcgggcaa tcgtatagac tttggcaatt tcggaggctg tcagattgcg gtagctctcc
    29341 gccgtaacct ttttactttc cagccactcg attgcctttt ttggcggcag acttaaggca
    29401 aatcccaaat caggcgcggg catggtcttg tcccaaaata tcgctgataa acaaggcctg
    29461 ctgcatatag gtacgcagtt tcgcgttgtc caaattcgga taaagcgcat tcagtgcggc
    29521 atctgcctcc tcgtagctgt tgcaagcatt aagtgcggca accgcctgac gcaccatcgg
    29581 gttgagctga gaattgaaat cgggctcaac cagcgcgtca tccaacgcgc cgtccaatat
    29641 ttcctgatgc ctgcccgtag ccttagatgg tacggtgtgg gcggataaag ccgccaatgc
    29701 agttctgttt atcggattgt ccggtacctg ccgcaccaac acagcctcac cctcctgcac
    29761 atctggaatg accagtttgt cgcgcaccca gctttcgggg atttgtacgc cgacatccac
    29821 cagtttcggg atagcgtcgg caaagaccgc gatgtctttc ggctcgcgcg tgtcaaattc
    29881 aaatttcggc acgcggtttg ggtcggcatt gggatagttg atttgcagga acggtccgat
    29941 gatttggctt gtgatggttt gcgccacctg ttttgcgtcc gacaccagca aatcgcggcg
    30001 tacctcgttg tggatattgc ccagcgcgtt ggtgctggat tttccgtccg caccgctggt
    30061 tagcgtttgc cccaaaatca gccgcgccgc cgatttttcg caccagtcgg ccatctgcaa
    30121 aaacggattg ctggttgcca tcgtaccgct tgcagcgttg tgcagctcga tttccatacc
    30181 ttctggcatg atgcctgccg cgttgtgacc gatttccgcc accgctcgaa gcagggtgtt
    30241 tttttcctct ttggttgcgc ccgcgccgta tttgccgata cggatgggca tgccgtacag
    30301 ctccaaaaac tcggcaaaat cgtggacggc gtagtgtttg aacatataca gccaggaaag
    30361 cgtgcggaaa agcccgttgc gcgcctgctg gacgctgcgc gatttttggg tatgaacgac
    30421 ccagcccagc ggccacaacg cttcgccttc cggattttcg cgggtacgca gcagcagccc
    30481 gttgtctttg tcccatttga accagctttg cgggcggtgg ataaagtttc ggggtaggta
    30541 aaggccgtct gaaaataccc actcgacctc caacgcagaa aatccgtgcc ctaccgcgtc
    30601 catcaaatcc ataatcaggt cttcgagggt aggcaggctg tccatcattt cgtaggcttg
    30661 gtcggacagc ttttcttctt cgggcgtcgc atttcgcggc ggggcgacgc gccagttgag
    30721 cgtcagcagc gcgcgtttgc gcgtccccat atttgccgcg atgtcgctgt cgcgctcctc
    30781 aatgtccgcg aaaagctcgt gttgggcgcg gatgtcgccg ctttccgcat cctcgaagag
    30841 ggcccgcatc ttttgcggtg taataaaatt ggacagatgc tcggcgataa cccgaccggt
    30901 cgccgtaatt tgagccacgt ccgtctgcaa tgcagcttcg ggcttttgga ttttagtttt
    30961 attgttcttt tttgccatta tcggctccat ttacttttta tctcgttacc aaaatcgtta
    31021 tcggtagggc tttgccattc tatcggcaca caattggcca ccgcccccga ccagagcata
    31081 tgcactgcat caggaccatc atcatgatca gcctttggaa aatggcggaa ctgctgtatc
    31141 agcgtctgtt ggctctcatt caacagaatc aaaccgttcg ccatgtgagg ctgtaaagtc
    31201 tcaatccgca agagcttgtc cgataccggc ttgaccgccc gcgccgggac aggtattcca
    31261 cgcgccgcgc tgcgcttgac cagctcatct ttcaaaaatt cctgaaactg aaccgtctca
    31321 acaaaccaca gtttgcaacg atattgacgg tgcaatctga taacgtcctc aataatcaaa
    31381 tcagggagac gttttttaat ctgagcttcc acgacataca gtttgccggt ttcacgttga
    31441 taaccgccaa tgataatcgc cgacgggtca cggctcgccc ccgctttacc cagcgacggg
    31501 tcgagcgcac cgaaatacac caaatcggac ggcaggtcgt tccaaaactt catcgacttg
    31561 gcaaacggcg catcttcgcc gctgaccggg tcgttttgat attcgctgtc aaatgtcgca
    31621 tgaccgtcac gcgcacggat tttcatcagc gcgagtacgc cgcgagccgc ccagcttgtt
    31681 tgcgcaccgc gctccatctc gtctttattg gcgagataaa acgcctcggc cacagtctcg
    31741 ccgtcgtttc ggaaaagttc ctcccatctg tcccacaaat ccatgcggtc gggccagcgt
    31801 ttcatcgcct taaacttaat accgtgccaa aacgggttat tcaaagtgcg gttaagtacg
    31861 ctgtcgtaat gcaaaatcgt gccgatatat atcacatcgt atttctggcc gacaccgccc
    31921 aaaggcaata cagtcttagt cagccacgca ttgagcttgt cgcgctgttc ggggttgcgg
    31981 acttgctcgt cattctcaat atcgtccaat atggtcaggt caggacggta agggccgtga
    32041 cgcaaaccgc gcagcttttt accgctaccg gccacttgga ctttaacgtc attggccgtc
    32101 acaatcgtac cggcctgcca tacgcggccc tgcccgcata cttccggaaa gtcggttttc
    32161 aagcgcggat taaattccaa ttccgccttg atggcttcga gcatcgggta tgcctgatct
    32221 atgctgtcca tcacaataac ggcataatgt ttttggcctg tcacaataca ccacagcgta
    32281 aacaactgag taacctgcgt cgacttaccc tcgccacgcg gcgcacccac cgcctcattt
    32341 tcccctttgg gggagcggat aatctccggc agacggctga ataaaaacgc atgcagttcg
    32401 gatttttcag gggagcggat ataatgcgga aagtaggtgt tgacgaaata ctcgtaaccg
    32461 cctaccgggt caaacacctt ggcacggcgt gcagcaatag ccttgggcga cgcatcgaag
    32521 ccgtccactt ccgcttcgat gacttggcgc aggctggcgg ccagttcggc aagggatttg
    32581 aggaattctt ttgttttcat gcatttaaaa agtcttaatc aatttttcgg caatccagcg
    32641 tgagaccggc ggacagacgg cgtttcccgc agcttgagcc tccgcagtgt tggcctcatc
    32701 cattccgagg agaagcccat catggttaga cgctctttgc cgctcaacca tcgcaccccg
    32761 tccgttgggc gtaacgagaa tatttgcgcc tgagcggtca atggctccat tggaaatccc
    32821 tctaagcaaa gtaccatgcg gcttttgcag cccgcacgtg tcaaccgagc gaggtaagac
    32881 tccaattgag ccggcgtcag ccataaactc ataggggggg gggtactctc ccaatccagc
    32941 gaccaagaaa actcgacggc gtgccgtggg gactccgaaa tatgctgcat taagcacgcg
    33001 ccagtatccc acatacccgc attcggcaag ggacttgatg actgtttgaa agtcttcgcc
    33061 attgttgcta ttgagcaaac ccgtaacgtt ttcaaggaca atccagcggg gtttaagctg
    33121 gtcgacaata cgcattgcgt cataaaataa acctgttctt tccccggcaa gacctttacg
    33181 cttaccggct attgatacat cctgacatgg gaatccgcca ataatcacat ctaccggcca
    33241 taattctgac aataccgttc tgacatcctc aaactgtttt gcgtatggga agcggtcggt
    33301 caaaactgca cggggtacag gattaatctc tacttgccaa gcagtgtcaa aaccagcctg
    33361 ttcaaatccc aaatcaaacc cgccaatacc tgcaaaaagg cttccgacgg taggtttttg
    33421 cttatccaaa cttcttctcc acctccaccc caaacggctc caatacctcc acaaaagcag
    33481 gcaaatgttt gggatgtttt tcttgcacaa acgccatcaa gaactcaatc aattccaaag
    33541 ccgtcgccag ttttgacgtt tccggcatca cacgcgcatt ggcggatacg gttttcgtaa
    33601 acgcatcggc caggctggcc aacagcttgg cgcggtccga tggcggcaaa tcttcggtac
    33661 tcgaatcctg cagcatcgtc atcgtgctgt tgtactgcac cataaaaccg gccaacatcg
    33721 cacggctcaa gtcttcaata ccgccgtcgg ccaaagtgta ggaggcgcgc atcttatccc
    33781 agtcgtcgcc tttttcctta tccgcacgtt tccacgcacg cgcagtggtc tgcgggattt
    33841 cgcacatcaa ggccgccgtt tccaaagttt gctcgccgct cacatagagc cggcgtaact
    33901 tttcacggat ttcttgcggg tgagccataa ttacagcccc attttcgctt taagcaattc
    33961 ccagccgacc gtaatcacgc cgccgcccag tgcgccgaat gtaatggccg tgcgtttcgt
    34021 gtcttggcgg atttgtgcaa tttccgcctg catttccttc tgatttttca gcgtttgatc
    34081 agtcttgttt tcaatacgcg ccaaggcttc caaaatcggg gcactcatga tttgtctgct
    34141 ttcctgtcca gtttttcgtt tactttttcc aacttgtttt cgatgcgtcc caacgaagcc
    34201 gcaatattgt cgcggtctgc cttggcatct tgcttggtgt gataagagag cttgaccgcg
    34261 tgcagctcct ctttcaggtc gtcgatacgc ttgtccgcct ctttcagacg gcctgaaatg
    34321 ccgttgaccc aaaaccaaaa tgccgccgtc gcaatcggcc acagggttct gaaaccaaat
    34381 tcaaagtcca tttaaaaccc ctttaaaccg gcacatcgcc gaatacgata cggacggcgt
    34441 aaccgtcagg gcgattgctg gcaatctcca gtccatcacc atcattacaa acacagtaat
    34501 acgcccccaa agtttgccaa accgcacgtt taaaggtgtt atagttcgta attggatatt
    34561 caaggtgaaa cgtcgtctga aaatccttat ccatgcgtac cgcgtactca atgcctgcct
    34621 tatccaacag atcggaaaca tgaatgacaa acggctcttg ttcgcgtgcg cggcttaagc
    34681 ccaattccaa atcggcatgg cgcacagcca actgacgttc aactaattca cggtaggtca
    34741 ttctttgata cccattaaat attttgtccg cttgtacaat ttcttaaccc acgaaatatt
    34801 tacaaatgta taaacctttg ctacaacttc gccgtcatag tgcgcatttt ctcgtgcagc
    34861 ccgaaatttc gccctggctt cttcagggtt gtccgcccaa atgctcaatg accaggactt
    34921 gccgttaaag cggtaagaaa acgtgtactc attcatagga gaaaccttat gtattttgaa
    34981 atctataaag acgcaaaagg cgaataccgt tggcgtttga aagcagccaa ccatgaaatc
    35041 atcgctcagg gcgaaggcta caccagcaag caaaactgcc agcacgcagt cgatttgctg
    35101 aaaagcacta ccgccgctac ccctgtaaaa gaggtataaa atccgctttt accctcagcc
    35161 cgcgccctac gcgggctttt ttgtcagttt ccgactttgc gggagtgatt gcccgcccag
    35221 tcgcgccaag cctgattttg gttttcaagt tcggcaacat agccgccaaa ctcagcggcg
    35281 tgttccaaca gcgttactgt cttgccgtct ttcggcggat tcgggcgtac cggcgcaacc
    35341 atcaatgcgg caggcggtgt cggcaagacc gccttttcga caaccttaat ttccgtagcc
    35401 gagggcgcgc ttgtagagct gcaagccgtg agagccaaag ccgtcaatac aaccgccgcc
    35461 tgcattttta cggtcttgag taaggacatt ttcgatttcc tttttatttt ccgttttcag
    35521 acggctgact tccgcctgtt ttttcgccaa agccatgccg acggcgtgcg ccttgacttc
    35581 atatttttta gcttccgcac gcgcctgttc cagctcgcgt gcatagtttt gagccgacaa
    35641 cagcagggct tgcgctttgt ctttttccat cttgtcaatg accgcctgct gcttcgcaaa
    35701 agccgacttg tagcc
//
LOCUS       DAWFAN010000003        35156 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWFAN010000003 DAWFAN010000000
VERSION     DAWFAN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35156
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.003"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(145..235)
                     /locus_tag="WGO65_000101"
     tRNA            complement(145..235)
                     /locus_tag="WGO65_000101"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Ser,seq:gga)
     gene            complement(274..360)
                     /locus_tag="WGO65_000102"
     tRNA            complement(274..360)
                     /locus_tag="WGO65_000102"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(324..326),aa:Leu,seq:cag)
     gene            complement(396..482)
                     /locus_tag="WGO65_000103"
     tRNA            complement(396..482)
                     /locus_tag="WGO65_000103"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(446..448),aa:Leu,seq:cag)
     gene            571..2946
                     /gene="rnr"
                     /locus_tag="WGO65_000104"
     CDS             571..2946
                     /gene="rnr"
                     /locus_tag="WGO65_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ0326471.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRQ
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3285..4748)
                     /gene="guaB"
                     /locus_tag="WGO65_000105"
     CDS             complement(3285..4748)
                     /gene="guaB"
                     /locus_tag="WGO65_000105"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ0326472.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     ALIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRNRLV
                     TVPEGTSIDEAREIMHAHKVERVLVLNDQDELKGLITVKDILKTTEFPNANKDAEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVSEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSADKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4895..7453)
                     /gene="glnD"
                     /locus_tag="WGO65_000106"
     CDS             complement(4895..7453)
                     /gene="glnD"
                     /locus_tag="WGO65_000106"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ0326473.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVGELCESVRDDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGISDARQFAADHF
                     LTEEESDLLAWLVENHLLMSIVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLT"
     gene            complement(7502..7822)
                     /locus_tag="WGO65_000107"
     CDS             complement(7502..7822)
                     /locus_tag="WGO65_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0326474.1"
                     /translation="MMGNKLTPPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            complement(8147..8620)
                     /gene="bfr"
                     /locus_tag="WGO65_000108"
     CDS             complement(8147..8620)
                     /gene="bfr"
                     /locus_tag="WGO65_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0326475.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(8648..9112)
                     /gene="bfr"
                     /locus_tag="WGO65_000109"
     CDS             complement(8648..9112)
                     /gene="bfr"
                     /locus_tag="WGO65_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0326476.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(9576..10559)
                     /gene="lipA"
                     /locus_tag="WGO65_000110"
     CDS             complement(9576..10559)
                     /gene="lipA"
                     /locus_tag="WGO65_000110"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ0326477.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(10552..11175)
                     /gene="lipB"
                     /locus_tag="WGO65_000111"
     CDS             complement(10552..11175)
                     /gene="lipB"
                     /locus_tag="WGO65_000111"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ0326478.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11177..11452)
                     /locus_tag="WGO65_000112"
     CDS             complement(11177..11452)
                     /locus_tag="WGO65_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ0326479.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            11637..12707
                     /locus_tag="WGO65_000113"
     CDS             11637..12707
                     /locus_tag="WGO65_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ0326480.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(12814..13761)
                     /locus_tag="WGO65_000114"
     CDS             complement(12814..13761)
                     /locus_tag="WGO65_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ0326481.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(13776..14183)
                     /locus_tag="WGO65_000115"
     CDS             complement(13776..14183)
                     /locus_tag="WGO65_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ0326482.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            14264..14923
                     /gene="ung"
                     /locus_tag="WGO65_000116"
     CDS             14264..14923
                     /gene="ung"
                     /locus_tag="WGO65_000116"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ0326483.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            complement(15431..15946)
                     /locus_tag="WGO65_000117"
     CDS             complement(15431..15946)
                     /locus_tag="WGO65_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326484.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(16412..18334)
                     /locus_tag="WGO65_000118"
     CDS             complement(16412..18334)
                     /locus_tag="WGO65_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0326485.1"
                     /translation="MIEIKNLTLQRGLKILLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALLKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(18411..18806)
                     /locus_tag="WGO65_000119"
     CDS             complement(18411..18806)
                     /locus_tag="WGO65_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ0326486.1"
                     /translation="MMRRRGKKMLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            18979..20286
                     /locus_tag="WGO65_000120"
     CDS             18979..20286
                     /locus_tag="WGO65_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ0326487.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREVRISAVCD
                     LSEEKARQICPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGHDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            20279..20713
                     /locus_tag="WGO65_000121"
     CDS             20279..20713
                     /locus_tag="WGO65_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326488.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(20772..21041)
                     /locus_tag="WGO65_000122"
     CDS             complement(20772..21041)
                     /locus_tag="WGO65_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0326489.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(21223..23673)
                     /gene="lon"
                     /locus_tag="WGO65_000123"
     CDS             complement(21223..23673)
                     /gene="lon"
                     /locus_tag="WGO65_000123"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ0326490.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALLGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHVHVP
                     EGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            23924..25027
                     /locus_tag="WGO65_000124"
     CDS             23924..25027
                     /locus_tag="WGO65_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0326491.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQADLRVADVL
                     KTKPVHKKAADPDDPLAFARNRETTFLYRLKKA"
     gene            complement(25099..26844)
                     /gene="recD"
                     /locus_tag="WGO65_000125"
     CDS             complement(25099..26844)
                     /gene="recD"
                     /locus_tag="WGO65_000125"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ0326492.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSGSEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQPFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(26912..27607)
                     /gene="lolD"
                     /locus_tag="WGO65_000126"
     CDS             complement(26912..27607)
                     /gene="lolD"
                     /locus_tag="WGO65_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ0326493.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRLLLMGEDLRTLSQRQLGILRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(27600..28847)
                     /locus_tag="WGO65_000127"
     CDS             complement(27600..28847)
                     /locus_tag="WGO65_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ0326494.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYNNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            29052..29330
                     /locus_tag="WGO65_000128"
     CDS             29052..29330
                     /locus_tag="WGO65_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326495.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSDSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(29386..30003)
                     /gene="recR"
                     /locus_tag="WGO65_000129"
     CDS             complement(29386..30003)
                     /gene="recR"
                     /locus_tag="WGO65_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ0326496.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(30070..31608)
                     /locus_tag="WGO65_000130"
     CDS             complement(30070..31608)
                     /locus_tag="WGO65_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ0326497.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAMQNEQADGGSPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(31663..32037)
                     /locus_tag="WGO65_000131"
     CDS             complement(31663..32037)
                     /locus_tag="WGO65_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ0326498.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAGTQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGRV
                     YVGFSEETYDGIFKRQAPCRQG"
     gene            32127..33815
                     /locus_tag="WGO65_000132"
     CDS             32127..33815
                     /locus_tag="WGO65_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ0326499.1"
                     /translation="MLQSENSKNLISCSIKALPIMISTNGITMQFGAKPLFENVSVKF
                     GEGNRYGLIGANGSGKSTFMKILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVL
                     DVVMMGHTEMWAAMTERDAIYANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLS
                     GVGISEDLHNAKMAEVAPGFKLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVL
                     NQYDSTMIIISHDRHFLNEVCTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNA
                     KAKEKLQELQEFVARFSANKSKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEAD
                     EKAKLHRQAVEVEKLAKLFETQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAY
                     NPEYSDGLLPDEGTIKWAEKASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIR
                     GTLGRLLFGSNDVVKKVKVLSGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESL
                     NMALEKYNGTLIFVSHDRQFVSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            33887..35140
                     /locus_tag="WGO65_000133"
     CDS             33887..35140
                     /locus_tag="WGO65_000133"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ0326500.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFSA
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEVANQVDSGKIAAECRAQGKVHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT         7759 a         9080 c         9587 g         8730 t
ORIGIN      
        1 ataccgcaaa acataccata aaaaaaacgg taacggatag ttgtaagcgg ttgatgacga
       61 ttatagacaa taacgggttt tcccaatgaa attattgttt gaataataaa aaatcccaaa
      121 ccgtaaaagt ttgggatttg tatttggcag agaggaaggg attcgaaccc tcgatacgct
      181 attcacgtat acacgctttc caggcgtgcg acttaaacca ctcatccacc tctctaatgg
      241 cggaaattat cccaatcggg ataatttatt atttggtgcc cgggagaaga ctcgaacttc
      301 cacacccatg aggataccag cacctgaagc tggcgcgtct accaattccg ccacccgggc
      361 aatctaaata ttgaaataaa gcaaagcatt tgatttggtg cccgggagaa gactcgaact
      421 tccacacccg tgaggatact agcacctgaa gctagcgcgt ctaccaattc cgccacccgg
      481 gctttgcttt atttgctgtt ttgcggtaca gtgtcctgcc gcaaagaagt cgctattata
      541 tagttcataa agagaatgtc aacagtccaa atgaataaaa atattaaatc tttaaattta
      601 cgggaaaaag acccgttttt aagtcgtgaa aaacagcgtt atgaacatcc tttgcccagt
      661 cgggaatgga taatcgaatt gttggagcgc aaaggtgtgc cttcaaaaat cgaatcgctt
      721 gcgcgcgagc tgtcgattac ggaagacgag tatgtctttt tcgaacgccg tctgaaggcg
      781 atggcgcggg acggtcaggt tttaatcaac cgtcggggcg cggtttgcgc ggcggacaaa
      841 ttggatttgg tcaaatgccg cgtcgaggcg cataaggacg gtttcggttt tgccgtgccg
      901 ctcacgcccg ccaaagacgg tgattttgtt ttgtatgaac gccagatgcg cggcattatg
      961 cacggcgata ttgtcactgt tcgtcctgcc ggcatcgacc gtaggggccg ccgcgaaggg
     1021 acagttctgg atattgtcga acgcgcgcaa agcaaagtgg tcggccgttt ctatatggat
     1081 aggggcgtgg cgattttgga gccggaagac aagcgtctga accaaagcat cgtattggaa
     1141 ccggacggcg tggcgcgttt caaacctgaa tccggtcagg tcatcgtcgg cgaaattgag
     1201 gtttatcctg agcaaaaccg gcaggcagtg gcaaaaatca tcgaagtttt gggcgattat
     1261 gccgacagcg gcatggagat tgaaattgcc gtgcgcaagc atcatttgcc gcaccaattc
     1321 agtgaagcgt gtgccaaagc cgtgaaaaaa attcccgacc atgtacgcaa aagcgatttg
     1381 aaaggccgcg tcgatttgcg cgacctgcct ttggtaacga ttgacggcga aacggcgcgc
     1441 gattttgacg atgcggtgtt tgccgaaaaa atcggacgca attaccgtct ggtcgtggcg
     1501 attgccgatg tcagccatta tgtccgcccc gatgacgcta tcgacacgga cgctcaggaa
     1561 cgcagcacca gtgtttactt cccgcgccgc gtgattccca tgttgccgga aaacctgtcc
     1621 aacggcatct gctcgctcaa tcctaatgtc gagcgtttgt gtatggtgtg cgatatggtc
     1681 atcacttacg cgggcaatat taaagaatac cgcttctacc ccgccgtgat gcgctctcat
     1741 gcccgcctga cctacaacca agtttggaaa tggctttcag gcggcatcgg gcatccgttc
     1801 aaaacacaaa tcgacacgct ttacaaactc ttcaaaatcc ttcagaaaaa gcgtttcgaa
     1861 cgcggggcgg tggagtttga cagcatcgaa acccaaatgc ttttcgacga caacggtaaa
     1921 attgaaaaaa tcgtccccgt tgtccgcaac gatgcccaca agctgattga agaatgtatg
     1981 ctggcggcga atgtttgcgc ggcggatttt ctgttgaaaa acaagcatac ggctttgttc
     2041 cgcaaccatt tgggccccac gcccgaaaaa ctcgccaccc tgcgcgagca gctcggtctg
     2101 ttggggcttc aacttggcgg cggcgacaac ccgtcgccga aagactatgc cgcgcttgtc
     2161 gaacaattca aaggcagacc tgatgccgaa ttgctgcaag tcatgatgtt gcgctccatg
     2221 cagcaggcgg tttacgaacc gcattgcgac ggacactttg gtcttgccta cgaagcatac
     2281 gcccacttca cctcgcccat ccgccgctat cccgacctga ccgtacaccg cgccatcaaa
     2341 gccgtgttga atcagcaaac ctacacgcca aaaaaaagct ggcaggcttt gggcgtgcat
     2401 acctcgttct gtgagcgccg tgccgacgac gccagccgcg acgtggaaaa ctggctgaaa
     2461 acctattata tgcgcgataa ggtcggcgaa gtattcgaag gtaaaatctc cggcatgacc
     2521 agttttggta tctttgtaac actggacggc atccacattg acggcttggt gcatatcagc
     2581 gatttgggcg aagactattt caacttccgc cccgaaatca tggcaatcga aggcgaacgc
     2641 agcggcatcc gtttcaacat gggggacagg gttgccgtcc gggtcgcccg tgccgatttg
     2701 gatgatggaa aaatcgattt tgtcctgatt gccgagggga gcggcagggg gcggaaagtt
     2761 aaatcatccg cgtctgccaa accggcaggg acggcgggga aagggaaacc gaaaaccgcc
     2821 gccgagaaaa aaacagcccg aggcggcaaa gtaaggggaa ggggcgcgtc tgccgccgca
     2881 gaatcgggga aaaaggcaaa gaaaccggtt ccgattaagg taaaaaaacg gaaaggcaaa
     2941 tcataatgct gacggggtgg cttggggagg gcggggcata ctttcccgaa gccgccgccc
     3001 gattttttga tatggcggcc ggcgcatcat ttcaggccaa tcgaaacgcc cgtattgaaa
     3061 gattgcgttt atttccaccg ccgttttaaa ggccggcggt attcggcaga cggggcgcaa
     3121 acggcgttca gacggcattt tcattctttc ggcgtgtccg tccgaattgc tttgcccgtc
     3181 cgcgcaatca gcccgtgcgg cttgccttga acggacaaaa aatgccgtct gaaacccgaa
     3241 aatcaggttt cagacggcat tttgccttga aaaggctgtt caaatcagcg gtggtagttc
     3301 ggtgcttctt tagtgatttg aacgtcgtga acgtgcgatt cgctcatacc tgcggaagtg
     3361 atttccacaa attcggcttt ttcgtgcatt tcggcaatat tggcgcaacc caagtaaccc
     3421 atactggaac gcagaccgcc ggtcagctgg tggatgatgt tcacaatcgg gcctttgtaa
     3481 ggaacgcggc cttcgatgcc ttcggggacg tatttgtcgg cgctgtcggt tttgtcttgg
     3541 aagtagcggt cggcagaacc ttggctcatc gcgcccaatg aacccatacc gcgataggat
     3601 ttgtaagagc ggccttggta gagttcgatt tcgcccggtg cttcttccgt accggcaaac
     3661 ataccgccga gcatgacgct gtatgcacct gctgccagag ctttggcaat atcgccggag
     3721 aagcggatac cgccgtcggc aatcagggga acgcctgtac ctttcagggc ttcggaaaca
     3781 ttgtgaatcg cagtcagttg aggtacgccc acgccggcca cgatacgggt cgtacaaatc
     3841 gagcccgggc cgataccgac tttaacggca tccgcacctg cagcgaccaa atccaaagcg
     3901 gctttggcag tcgcgatgtt gccgccgatg acttggatgt gcggataagt ttctttaacc
     3961 cagcgcacgc gatcgataac gccttggcta tgaccgtggg cagtatcgac cacaatcacg
     4021 tctacgccgg cttctaccaa tgctttgacg cgctcttcgg tatcgccgcc agtacccacc
     4081 gcggcaccga cgcgcagacg gccttcggca tctttgttgg cattagggaa ttcggttgtt
     4141 ttcaaaatat ctttaactgt aatcagacct ttgagttcgt cttggtcgtt caacaccaaa
     4201 acgcgctcaa ctttgtgcgc atgcatgatt tcgcgggctt cgtcgatgct ggtaccttca
     4261 ggtacggtca ccaaacggtt gcggggggtc atgatggcag aaaccggcag atcgacacgg
     4321 ttttcaaaac gcaggtcgcg gttggtcacg ataccgacca ctttgccgtt ttcaacaaca
     4381 ggcagaccgg acattttgcg tttgcgttgt gcgcgcattt ccaagacttc gcggatcaat
     4441 gcggtcggcg caacggttac ggggtctttg accacgccgc tttcgtggcg ttttactttg
     4501 gaaacggcgc gcgcctgcat ttcgggcggc atgtttttat ggatgatgcc gatgccgcct
     4561 tcctgtgcca tcgaaatggc gaggcgcgcc tcggtaacag tgtccatcgc ggcggaaagc
     4621 agggggaggt tgagtgtgat ttcgcgggtg agcttggttt gaagtttaac gtctcgcggc
     4681 agcacggtcg aatgtgcggg aaccaacaaa acatcgtcga aagtataggc tttttctacg
     4741 atacgcataa tgctcggtct ttcagtttgt gcaagatgca cggcattata gcacgttacc
     4801 ggcggcttga cagtttatat tgtttgcaat atcccccgcc ttcagacagc gtacggcgcg
     4861 gcggcgggat accgtctgaa aaccggcagg gtgcttatgt caactgttcc agcaaagcct
     4921 gcttcaatga ggactggatt tttgggtttt tcaggtcggg gctaaaaacg gtaaaactgt
     4981 cttcggcgcg ttcatccagt gtggagattt tggcatagcg caggctgacg ttgtgggcga
     5041 aaaagacttc cgccatatcg gcgagcagga aggggcggtt gacggcggta atttcgacgg
     5101 agtaccagtc cggatagtct tcttcggggg tgatggtgat gcttggcgcg atcggcatgt
     5161 agcggctgcg gcggctgatg cggcggctat ggctggagat ttcggcaacg gtgtgtccgt
     5221 ggataaagct gttgagttcg gcttcgagcg cgctttggat gtcggggtag tcttcgggag
     5281 cgtgctgcga ggggatttgc acgatgaagg tgtcgaggat gtagtcgtgt tcggtaataa
     5341 aggcgcgggc ggcgaggatg tcgaagccgt ggcggctgaa gatgcggcag aggcgggcga
     5401 acaggcgcgg gccgttgggc atgaaaacca tgacttgaaa gctgtcgctt ttgggcagga
     5461 tgcggctgcg gacgatgggg gtttcaaagt cgtggactag gttggcggcg tgccacagga
     5521 tttcgcggga ctggtggcgg gcgaagtagg cggaaccgag cgcgttccat agttttttct
     5581 gctgtttttc ggggacggcg gcgcgggtga gtaagtcggc ggcttcctgc cggcggcggc
     5641 cgaagagggt gtgcgggttg ccgccgttgc ctgtaaggta gcgtccggcg gcatggaaga
     5701 ggctttccag caggctggcg cgccatgcgt tccacagctt gggattggta ccgcgtatgt
     5761 cggaaatggt cagaaggtag agcgcgctga ggcgttcgtg ggtttggacg cgtttgcaga
     5821 aggcatcgag tacgtcgggg tcttggatgt cttctttttg ggcgacgatg gacataagaa
     5881 gatggttttc gaccagccag gcaagcaggt cgctttcttc ttcggtcagg aagtggtcgg
     5941 cggcaaattg gcgcgcgtct gagatgcctt gtatggcatg gtcgccgccg cgtcctttgg
     6001 cgatgtcatg gaaaaaggcg gcaaggtaga ggatgtcttg tttttcaaag gactgcatca
     6061 gtgcagaggc gtagggcagc tcgtggctgt gcatatccaa ggcaaggcgg cggacgttgc
     6121 ggacgacggc gaggatgtgg tcgtccacgg gatagatgtg gaacaggtcg tgctggagca
     6181 ggccgacgat tttttcccac gcgggcaggt agcgtcccaa tacgccgtag aggttgagga
     6241 agcgcagggt ttgggtcagt ccgttgccgt tgcggaaaaa gccggcgaag cggtggcggt
     6301 tttcagaatt ttggtagaag ctgcggttga ttttgcgcgt cgcgccccac catgcgcgca
     6361 gggtttgcgg ttcgagcgcg gtaatgtcgt tgcgctgctg catgatttcg acgattttga
     6421 aaatgtgttc gggccgtctg aaaaaaatat cggtgtgccg cgcggcgatt tggttgttga
     6481 cttggatgta gtcgtcgtca atccgcaggg taacgcgcaa cggggtggag gaaacgcggc
     6541 tttgcagcat aggcgtgagg atgccgccca gttgtttgac ggttttaatc gcgcggtaaa
     6601 acacgcgcat cagttcttcg ctttggcggc ggaggttcaa gccttcataa cccatgcttt
     6661 cggcgacttg cggctgcaaa tcgaacagca ggcggtcttc ggcgcgcttg gcgtttaaat
     6721 gcagatggat gcggatgtgg gcgaggcggc ggtagccgtg cgaaagcata ccggcttcgg
     6781 cacgcgtcaa aatccgctgt ttgagcaggt cgggcaggtc ggtcgccaag ccttgcgcct
     6841 tcgctatcca aagcagggtg tggatatcgc gcagaccgcc cggacagctt ttgatattcg
     6901 gctccaatac tgcccccgaa ccttgcgatt tggcgtggcg gtgttccatc tccaccagtt
     6961 ttgcctcgac aaacgccgcc acattgcgct gcgcgttcat tttttccgct agtttatccg
     7021 ccgtttggcg gttgccaaac aaaaacctag cctctaaaaa cgccgtgtcc cccgtaatat
     7081 cgtcgcgcac gctttcgcac agttcgccaa cgctgccgct ttttacagac ggcatcagtt
     7141 tgcagtccca cagggtttgg acaaaccggg caatctgttc ctgaatgccg tctgaaagcg
     7201 gggcagggga gacaaccgcc aaatccacat ccgaacaggg atacagttcg ccgcgtccga
     7261 agccgcctac cgccatcagg cataacgcgc tgttttgaaa atactctgcc cacaatgccg
     7321 ccagtaaggt ttcgactgcc gccgtgtatt ctctgaaaaa taccgacacg cggttggctt
     7381 tcaaataatg cgcttcggcg gcatcgcgct gctgtttgaa ggtttccagt gctgaagaca
     7441 ggtttgcagg catttttatt ctttcgattg gcgggaaaaa gggaggcgga tggttcggcg
     7501 gtcaaatacc gctttcagac ggcatttgtc gggtatcggc ggaattggtc atcaggttca
     7561 accgttcctg cggcggcagc agcaactgat acgccgccac gcccaaagcc gccgccattt
     7621 tttcgatatt cgacagggcg atgttccagc gtttgcgttc gactgccgac acataagtcc
     7681 tgtccaaacc gcattgccgc gccaattctt cttgcgacca acccttgttc acgcggaaaa
     7741 gccgcatatt gtatgccaat accgcccgca aatcctgttc gtcaggcagt tcggcaggcg
     7801 gggtcaattt gttgcccatc atttgcttcc agataaatgg ttaaaattaa gcatttgctg
     7861 ttacggattt tacttcacat aaaagccaaa aaaaaggggt ggcggcagcc acacccaaac
     7921 acacacacat caagaggaaa gaggtaaaat caagacagat tgggaaacag caaacaagca
     7981 atctagattt catatcggta ttatcaaaca aaaaccgtta tcgagtattg attcaaatca
     8041 atttagattt tattcgcaaa ccggcaaaga aaaacggcat acccgtttat acggatatgc
     8101 cgtctgatga tgcaggggac tgctgtattt ggcagtggtt tgtgttttag tcctcttgcg
     8161 ccgctgtttg caggtagttc ggcaaaccaa ttttgccgat tagttcctgc tgggtttcga
     8221 gccagtcgat gtgttcttcg ttggtgtctt tttgtttttc caacaaatca cggctgacgt
     8281 aatcctgttg cgcttctgct gtggcgatgg cggcaagcag ggcttcgtgt ttttcctgtt
     8341 ctttggtcaa atcgcaggcg atgatttctt cggtggactc gccaatcaga agcttgccca
     8401 gttcttgcag gttcggcagg ccttcaagga acagaatgcg ctcgatcaaa tcgtcggcag
     8461 ctttcatttc aacgatggac tgtttgaaga aatgttcgcc cagttcttca aagccccagt
     8521 ttttcaaaat acgggcgtga aggaaatatt ggttgatggt taccagcagc aagcctaagt
     8581 ttttgtttag ctcgcggata accagacggt cgcctttcat acggactcct tttattccga
     8641 atacggatta cagttggctt tggtagtagt tgccttcgcc aattaactcg atcaggcgaa
     8701 gctgctgttc cagccagtgg gcgtggtctt cttcggtatc tttcagttgg gcaaccatca
     8761 ggtcgcgcgt aacatagtct tgagcctctt cgcacagttt gatgcctttt ttcaacgcat
     8821 cacgtacttc atattcggtt tgcaggtcgg ctttgaggca ggaaaccacg tccgtgccga
     8881 tattcagttc ggcgcgtgcc attttcggcg tgccgcccag catcaggatg cggcggatga
     8941 agtcttcggc gtgtgtggtt tcttcttcca tctcgtggtt gagacgttca aaaagtttgg
     9001 tgtagcccca ttcggagtag aggcgggagt ggataaagta ttggtcgcgt gccgccagct
     9061 cgccagacag caattcgttc atataatcaa caacagcttg attgccttgc ataatatctc
     9121 tctttctgta acttggtttt cggtatgtct gaacagcatc tgtgtcgcta ttcgggatgc
     9181 gtgcattgta tgcaaaagct gtcggcatga caaattttct atttaaaata caaacaatta
     9241 tcaaaagaaa tagggcgtat tccccgatgc cttccaaatc agtatgctgt ttcttatcgg
     9301 ttttttgtgc tgttaaaact aagaatccat atcatccata aagaacattt acacttgtta
     9361 actatagcga aaaacaaata gagcacggtt ttttatcaaa atttataatg aatcgttctc
     9421 attaaccgac agattctttt gagattatcg gattttggga ataaattatg ccgtttgagg
     9481 ggctttcaga cggcatagac aattaaagct gattaaacaa cgcggcagac agcagcgtag
     9541 gaaattattt tttacttcgg aacggtattt tgggtttaat gatgcccgca accgccgtgg
     9601 ctttcgcgta aggcttcggc agcctgttcg tcggcgtggt agctggaacg taccatcgcg
     9661 ccgatggcgg cattgctgaa gcccagttcg tatgcttctt tttcaaagat tttgaactgc
     9721 tcgggcgtaa cgtagcgcag gacgggcagg tgtccgtctg aaggctggag gtactgtccg
     9781 atggtaatca tttcgatatt gtgcgcccgc atatcgcgca taatttcacg cacgtcttcg
     9841 tctgtttcgc ccaagccgac catgatgccg gatttggtcg ggatgtgcgg catcatttct
     9901 ttataacgtt ttaataagtc taaagaatgt tgataattgg caccgggacg ggcttttctg
     9961 tacaggctcg gatgggtttc taggttgtgg ttcatcacgt cgggcggggt ttcggcaagg
    10021 attttgagtg cgatgtccaa gcgtcctcgg aagtcgggga cgaggatttc gattttggtg
    10081 ttcgggctgg tttcgcggat ggctttgatg cagtcggcga aatgctgtgc gccgccgtcg
    10141 cgcaggtcgt cgcggtcgac ggaggtgatg acgacgtaac gcaggttcat ggctttgacg
    10201 gattcggcga ggtttttcgg ttcgtcgggg tcgagcatat tgggccgacc gtgtcccacg
    10261 tcgcagaacg ggcagcggcg ggtgcagatg tcacccataa tcatgaaggt cgccgtgcct
    10321 ttgctgaagc attcgccgat gttggggcag gaggcttcct cgcaaacggt gtgcatcttt
    10381 tgttcgcgca aaatgtcttt gatttcaaag aatttgcgcg atgggagttt ggcgcgtatc
    10441 cattcgggct ttttcagttt ctcctgaagg gggacgactt tgatggggat gcgggcggtt
    10501 ttgtccgcgc ctctgagttt gatgccgcgt ttggggtcgt cggttttgat ttcactcatt
    10561 gttgtctgct ttcggtgtga attgtgtttc aaggtgtgcg gtaagtttgg cggcgacttc
    10621 gtccggcgtg gggcagggtt ggacaaaatc cgcgatttgc gtcatttcca taccggcgta
    10681 gccgcagggg ttgatgtggg taaacgggct taaatccata ttgacgttga gcgcaagccc
    10741 gtgatagacg gagccgtttt tgatacgcag ccccagtgag gcgattttgc gttctccgac
    10801 ataaacgccg gggcgtttgg ggtctgccgc cgcttcgatg ccgtattctg ccaatgtggc
    10861 gatgatgctg ttttcaagcg cggaaacgat gttgcggacg ctggttttgc gccgtttgaa
    10921 atcaatcatc gtataaacga ccaattgccc gggcccgtga taggtaatct gcccgccccg
    10981 gtcgatttgg acgacgggaa tgtcgtcgcg aatcagcagg tgttcgggtt ttcccgccag
    11041 tccttgtgtg aacacgggcg ggtgttcgac gacccacagt tcgtcttcgg tgtcagcatt
    11101 ccgtccggca ttaaaggttt tcatcgcttc aaaagtcggc agatattcga ccaaaccttt
    11161 gtgtatgatt ttcatctcaa agtaccactt tgaccagttc gtgcgaagtc agcgcgcggt
    11221 agatgttgtc caattgttct tggttttcaa cctttacctg aacggtggcg ccggtatagt
    11281 tgcctttgct gctcggacgc gtggtgatgt ggtgcgcctg cgtgtcgggg gcgtggaggc
    11341 ggacggtgtc taaaaccgcc tgctcgaact cgggatgcac cgcgcccatt actttcaatg
    11401 ggaaggtgca gggaaattcg atgagggatg ttttgttttt ttgttcggtc atgatgtgct
    11461 gccttgtcgt gtacggtatg ccgtctgaag gcgggtttgc ctttcagacg gcatcggatg
    11521 tgcgttattt tagcctaaac cgcgataaca ggctatcggg aaggcggagg cttttttgac
    11581 ggcgcggcgg ttctgctata ctggcgcaca atattatttt tagaagggtg gtttttatgt
    11641 atcggaggaa agggcggggc atcaagccgt ggatgggtgc cggtgcggcg tttgccgcct
    11701 tggtctggct ggttttcgcg ctcggcgata ctttgactcc gtttgcggtt gcggcggtgc
    11761 tggcgtatgt attggaccct ttggtcgaat ggttgcagaa aaagggtttg aaccgtgcat
    11821 ccgcttcgat gtctgtgatg gtgttttcct tgattttgtt gttggcatta ttgttgatta
    11881 tcgtccctat gctggtcggg cagttcaaca atttggcatc gcgcctgccc caattaatcg
    11941 gttttatgca gaacacgctg ctgccgtggt tgaaaaatac aatcggcgga tatgtggaaa
    12001 tcgatcaggc atctattatt gcgtggcttc aggcgcatac gggagagttg agcaacgcgc
    12061 ttaaggcgtg gtttcccgtt ttgatgaggc agggcggcaa tattgtcagc agtatcggca
    12121 acctgctgct gcttcccttg ctgctttact atttcctgct ggattggcag cggtggtcgt
    12181 gcggcattgc caaactggtt ccgaggcgtt ttgccggtgc ttatacgcgc attacaggca
    12241 atttgaacga ggtattgggc gaatttttgc gcgggcagct tctggtgatg ctgattatgg
    12301 gtttggttta cggcttgggg ttggtgctgg tcgggctgga ttcggggttt gccatcggta
    12361 tgcttgccgg cattttggtt tttgttccct atttgggcgc gtttacagga ctgctgctgg
    12421 caaccgtcgc cgccttgctc cagttcggtt cgtggaacgg catcctatcg gtttgggcgg
    12481 tttttgccgt aggacagttt ctcgaaagtt ttttcattac gccgaaaatc gtgggagacc
    12541 gtatcgggct gtcgccgttt tgggttatct tttcgctgat ggcgttcggg cagctgatgg
    12601 gctttgtcgg aatgttggcc ggattgcctt tggccgccgt aaccttggtc ttgcttcgcg
    12661 agggcgtgca gaaatatttt gccggcagtt tttaccgggg caggtaggcg gttccgaaac
    12721 atatttgaag cggaatacaa ccttgcccgg gtttaaataa aaatgccgtc tgaaccccga
    12781 aaacagagct tcagacggca ttttcatcac ggcttatttg gcggttttgc tgctgtcgat
    12841 aattttcata ccggcagaaa tcaggctgcc gatgtcggca acattggcgg gcataatcag
    12901 cgtattgctt tctttggcaa gattgttgaa cgcggcgacg tattgttccg caatcttcag
    12961 attgaccgca tccgcgccgc cttgggtttg aagggcggcg gcaatttgac ggatggcttc
    13021 ggcattggct tcggcaacaa ggcgcaagga ttccgcttca cctttggcgc ggttgatgcg
    13081 ggcgattttc tcggcatttg acgcattgac cgcagcctga gcctcgcctt cggattgttg
    13141 gatttcggct tcgcgctgac cactggcaag gttgatttgt tcgattttac gaccttcgga
    13201 ttcggcgata cgggcgcgtt tttcgcgttc ggcagtaatt tgcgcctgca ttgagcgaag
    13261 gatttcttgc ggcggaacca agtctttaat ctcataacgc aaaaccttca caccccaagc
    13321 cccggccgcc tcgtccaaag ccgcaacaac agtactgttg atttcgtcgc gttcttcaaa
    13381 cgttttgtcc aactccatac gcccgataac ggaacgcagc gtcgtttggg caagctgggt
    13441 aattgccatg atgtagttgc tcgaaccgta tgaggcgagt ttggggtcgg ttacttggaa
    13501 atagatgatg ccgtcaacag tcagctgcgt attgtcgcgc gtgatgcaga cctggctggg
    13561 tacgtctaaa gggatttctt tcagcgaatg gcggtaggca acgcggtcga taaagggaat
    13621 caaaatattc aaaccggccg tcagggcgcg atggaaacgc ccgagccttt cgacaacgtg
    13681 gacttcctgt tgtgggatga caacaaagga tttgaaaccg aaaacggcaa cggctgccaa
    13741 caagataatg aaaaattcca taattcctcc gagtgttaag gttttgcgat gataagaagg
    13801 ttgccttcct tgcggacgat tagggcgcgc gttcctggtt caagctcttc ttgccccgta
    13861 ttttgcgcct gccagtgcgt accgcgataa aaaacttcgt aacggttgcc gcctgtgtgt
    13921 cggaggattt cgacatattg tccggcatcc aaatcctgat atgaatccgt ttcaactttt
    13981 ctaacggcgg ttttggcgtg tacgaaccaa atacccagcg cggaaagcag agcggcggtc
    14041 aagacggcgg caggcgtgct gccggtcagc ccgtaagcaa tgcccgaacc cgccaaagcc
    14101 gcgctgacaa ccaaaagata aaccgttccc gtcaataatt cgatgattaa gacggcaaca
    14161 gcggcaacaa accatacagt catacatttc cccacaaagc gcgtcgtttg acaaaataac
    14221 gcaatatcag cagtatagcc gaatttgaaa ggatagggca gatatggaca cttggcacga
    14281 tgcactcggc ggcgaaaaac agcagccgta ttttcaggaa attttaaatg cagtcaggca
    14341 ggaacgtttg tcgggacaaa tcatctatcc gccggcggcg gatgtgttca acgcattccg
    14401 cctgacagcg ttcgaccggg tcaaagccgt cattctcgga caagatccgt atcacggggc
    14461 agggcaggcg cacggtttgg cattttccgt ccggcagggt atccgcatac cgccgtcttt
    14521 actcaatatc tacaaagaat tggaaaccga catcgaaggc ttttccatac ccgcgcacgg
    14581 ctgcctgaca gcgtgggcgg agcagggcgt attacttctg aacacggttt tgacggtgcg
    14641 tgcaggacag gcgcattcgc acgccctttt aggctgggaa cgctttaccg ataccgtcat
    14701 caggcagctt gcgacacacc gcaagcatct tgtctttatg ctgtggggtg ggtatgcaca
    14761 acaaaaaagg aaactgatag acagtcaaaa ccatttgata ttgaccgcac cgcatccgtc
    14821 tcctctgtcg gcatatcgcg gttttttcgg ctgccgccat ttttcacagg caaacagcta
    14881 tttgagccgg cacggtatcg atccgataaa ctggaagctg tgaatgccat ctgaatgcca
    14941 agttgcttaa accccactga aggctgcttt tgaaaccgga aacaaagtga ttaaggaaaa
    15001 ccggcccata atgcaaaacc gtctgaatcc tagggttcag acggcatttt ttgccgccat
    15061 ttccaaaaac aacttttgtt cgcctgccga caaatccgac aaagtcgttt ggttgagggc
    15121 tttggcaata atttaacata atatacatta tgcgaactat cggaaacagt tgtacgtgtc
    15181 cctgtggtct ttccaagtag gaaaattaaa gtatggacaa tgcggctgaa tgtatagccc
    15241 ggagcatccg cgtatccgaa ggtactgaac gatatgccct gcatcccttt cgatgcttcc
    15301 atccagccgg tattgggaaa tacgttgacc ttcaatataa tatacaggca gccgacacac
    15361 aggatatgcc gctgcttttt catcatttct tctgtcaaat ccttggaaca gttgatttga
    15421 aaacacggct ttacatacgt cccaattctc tttgcagtgc ttggatattt tgctgcctgt
    15481 ccaatacatt gctttgcaat gcgttgattt cttgatggtt gatgttgccg ccctttgcca
    15541 gacgtgcttg tgataacatt ttttgggctt caaccaatgc tttgcgttcg ttgctcaatt
    15601 ctgtttcgag aatggagcgt ctgctgttgt ttgagggtgc ttgttgcggc ggcggcgtat
    15661 tggattttgc cggcttggat actgttttga ccggggcttt atatttgaca acctgtccgc
    15721 cgtttgacgg tgatgatacc ggttcgggcg tttggggcgg gatatagcgt tcgctgctgt
    15781 agttgccgat tgggggcaaa tcggttgagt ggcagctttt ggacggcttg gtggtgtaaa
    15841 cggtttctcc gttgattgtg caggtgtaga ttttggccgc attcgcaccc aatgggcttg
    15901 aaatcaggga aaagttgatt aggattaagg ggagttttga tttcataatg tgcttgatgc
    15961 tcggataatt atttgatttt ttggtatttt ttataatgct ttcttggcgt tttacgggct
    16021 tttcttttcg gttgtttgct tttgattttc tttcggggct ttggcagcct gcttgttctt
    16081 tttcccggca ggttttttgt ttttcttttt actttctatg tgtattttgg cttcttaact
    16141 gagtttttta attttcaggc ggtatccgcc tccctgatgg ctgctgattt taggtaaatc
    16201 cgcatcggag cacgatcctg ctggggctga tgtatatacg cttttgccat ttgagttgca
    16261 atggtatacg gaggcttgcg ccgccgcgcc ggaaagggac aggagaccca aggcggcaaa
    16321 aagtctgatg tttataccgg taaaaggcgg tgataacgcc cgaattatac cgttattggc
    16381 aggcaaagat aagcaccctg cccgcgcttc tttatcgctc ggcaaactgt ttctcgattt
    16441 cagtttcaat ccgttccaat tcttcttgac aagccagcca aacctcttcg atttgggcaa
    16501 gttgtgtttt gacttttgcc agctgggata aggtattttg caatttttct ttgtttgctt
    16561 caaggtaagc gtcttcttgt gctaaaaatg cttcacatgc cgtctgaatt tcggaaagct
    16621 gcgccatttc tttttcggca cggtctattt tctgctgtat cggcttgccg cgtcgggctt
    16681 tttcctgacg gatttgcgct tcgatgcgct tggtgtcttt gcggctttgg ctttgtgcgg
    16741 atgctgcggg tgcggcggcg gcgttttcct gtgccaaacg ccattggcgg taatcgttca
    16801 aatcgccgtc gaagttcttc agacggcctt tgtcgatcag gaggaagctg tcggtagtcg
    16861 cttcaagcag gctgcggtcg tgcgatacga tgattaaggc gccttggaaa ctttgcagag
    16921 cgagcgtcag ggcgtggcgc atatccaagt ccagatggtt ggttggttcg tcaagcagca
    16981 gcaggttcgg cttttgccaa ataatcatgg caagggcgag acgggctttt tctccgccgg
    17041 aaaaaggttc ggttttctgc aacgccatat cgccaacaaa attgaagccg ccgaggaaat
    17101 ttcggatttc ttgttcgcgt acttcgggag aaagctgctg aatatgccaa acagggtttt
    17161 ggtcggcgcg gatggtatcg agttggtgtt gggcaaaata gccgatattg agtttttcgg
    17221 aacggacgat gctgccggaa agcaaatcga ttttgcccgc caaggctttg ataaacgtag
    17281 atttaccgct gccgttgaca cccaatagcc cgtagcgtgc gccgctttcc agcgacaggg
    17341 taatgtcatg cagaacggtt tttccttcgt aacccaaatc agcgtgttct agctttaaca
    17401 aaggattggg cagatggtcg ggatggtaaa actcaaagga aaactcgctg tccagatgcg
    17461 cgggcgcaac gcgttctagc tttgccaaag ccttcatgcg gctttgcgct tgaacggctt
    17521 tggtggcttt ggctttgaag cggtcgataa aggattgcaa atgtttgatt tgcgcctgct
    17581 gtttgacata ggcagcttgt tgttgcgcga gacgctgcgc acgttcgttt tggtaaaaat
    17641 cgtaattgcc gccgtattgc gtgagttttt gctgcgataa ttcaatggtt tgggtagttg
    17701 ccgcgttgag aaaatcgcgg tcatgggaaa tgatgatttg cgtgcagggt aaagaagcaa
    17761 ggtggttttc cagccacaag acggtttcca aatccaagtg gttggtcggt tcgtcaagca
    17821 agagcaaatc ggcgcggcaa atcagggctt gcgcaagatt caggcgcata cgccagccgc
    17881 cggaaaagga tttgacgggg cggctgtgtt cttcttgcga aaaacccagc ccgttcaaca
    17941 attttgccgc gcgggccggc gcggtataag cgtcgatttc ttccaattta gcatgatatt
    18001 ccgcctgctt catgccgtca ttttgcgctt ccgcctgcct caatgccgtc tgaaaagcct
    18061 gcaactcggc atcgccctgc aaaacgtaat ccaaagcgga aatatccaaa tcgggcgttt
    18121 cttgggaaac ggaagcgagc cgccagtttt tcggaatcga gacatcgccg ccgtcctgag
    18181 tgatttcacc cttgagtaag gcaaacaggc tggatttgcc cgtcccgttt ttgccgatca
    18241 aaccgacgcg ctgaccggga ttgacagtgg cgttggcttt gtcgagcagg attttcaaac
    18301 cgcgttgcag ggtgaggttt ttgatttcaa tcataacgga aacatcgtcg ggcggaaaaa
    18361 gcccgtattt tacctgaaaa tcagtgccga tgccgtctga aacgggaaat ttacggctga
    18421 agccaagccc aagccctgcg cccttccgag tgcaggaaaa ccaatgtcct gaatgccgaa
    18481 tcggtattca tgcattccac gctgattccg attcgggaaa aatccgccat gattttggga
    18541 tggataaact cctgagccgc gcccgtcccg ataatcaata tttccggata gtcaacaggt
    18601 ttgatgtcgg acaacaggtt ttccggagtc agatcggaca aggttcggca ttgcgacagg
    18661 cagaccgaat ccttatgtac aagcacgggt ttatggaaac tttgccccgc cagccggatt
    18721 ccgcccgcac cgcattcata ttccgcaaac tgtccgtcta tcggattttc ttcaaacaac
    18781 atttttttac cccgtcgccg catcatctac accgaaaggg gtgcaaaatc agacaaattc
    18841 atgtaggatt ggcagatttc atctgacccg cctgccgatt tcagacggca tttgattcaa
    18901 agtgcggcac aattatatcg gcagcggata ttttcgtctt tcaatattta catttcagtc
    18961 ggtttacaag gagacacaat gaagccagta aacatcggtc ttttaggttt aggtacggtc
    19021 ggcggcggta cggctgccgt gttacgggac aatgcggagg aaatttcccg ccgtttggga
    19081 cgcgaagtgc gcatcagtgc cgtatgcgac ttgagcgaag aaaaagcccg ccaaatctgc
    19141 ccgtccgcag cctttgtcaa agacccgttc gaactggtcg cacgtgaaga cgtcgatgta
    19201 gtcgtcgaat tgttcggcgg taccggcatt gccaaagatg cggtgttgaa agccattgaa
    19261 aacggcaaac acatcgttac cgccaacaaa aaactgctcg ccgaatacgg caacgaaatc
    19321 ttcccgctgg cggaaaaaca aaacgtcatc gtccaatttg aagcggcagt agcgggcggt
    19381 atcccaatca tcaaagccct gcgcgaaggt ttggcggcaa acaggattaa atccatcgcc
    19441 ggcattatta acggcaccag caacttcatc ctctccgaaa tgcgtgaaaa aggcagcgcg
    19501 tttgccgatg tactgaaaga agcgcaggca ctgggctatg ccgaagccga cccgaccttc
    19561 gacatcgaag gacatgacgc gggccataaa atcaccatca tgagcgcact ggcattcggc
    19621 acgccgatga acttttccgc ctgctacctc gaaggcatca gcaaactcga cagccgcgac
    19681 atcaaatacg ccgaagaact tggctatcgc atcaaactgt tgggcattac ccgcaaaacc
    19741 ggcaaaggca tcgagctgcg cgtccaccct accctgattc ccgaaagccg cctcttggca
    19801 aacgtcaacg gcgtgatgaa cgccgtgcgc gtcaacgccg atatggttgg cgaaacctta
    19861 tattacggcg cgggcgcggg cgcattgccg accgcttccg ccgtggttgc cgatatcatc
    19921 gacatcgccc gcctggttga agccgatacc gcccaccgcg taccgcatct ggcgttccaa
    19981 cccgcgcaag tccaagcgca aaccatcctg cctatggacg aaattaccag cagctactac
    20041 ctgcgcgtcc aagccaaaga cgaaccgggc acgctggggc aaatcgccgc gctgttggca
    20101 caagaaaacg tgtccatcga agcactgatt caaaaaggcg tgattgatca gaccactgcc
    20161 gaaatcgtga ttctgaccca cagcacggtc gaaaaacaca tcaagtcggc aatcgcagcc
    20221 atcgaagcac tggattgcgt ggaaaaaccg attaccatga tccgcatgga aagcctgcat
    20281 gactgagccg aaacacgaaa tgctgacgaa agagcaggtt gccgcgcgca aaaaagcaaa
    20341 agccaaaatc cgcaccatcc gcatttgggc gtgggtcatt ttggcgttgc tcgctttaac
    20401 cgccctgctc tcccaatgcg cgatgtccaa accgcaggca aaacagaaaa ttgtcgagtc
    20461 ttgcgtgaag aatattccgt ttgccgaaaa atggcaaaac gatttgcggg cccgcggttt
    20521 agattcaaac aatacccgcc tcgccgtcga ctactgcaaa tgtatgtggg agcagccttt
    20581 ggacagattg agcgagaaac agattagatc cttcggcaaa ctcggcgcac aagaacagct
    20641 tgacctgctc ggcggcgcaa atgcctttga agcacgtgac aagcagtgtg ttgccgattt
    20701 gaaatcagaa taatgtggac cgataaaaaa gccgattctt taaagaatcg gcttttttca
    20761 taaaaaacgg cttacagtgc gtctttcaaa gctttgccgg cgcggaattt aggcgttttg
    20821 gcggcggcaa tggtcagagg ctcgccggtt ttggggttgc ggccttggcg ttccgcacgt
    20881 tcgcccacgt agaaagtacc gaaaccgacc aaagtaacgg tgtcgccttg tttcagggcg
    20941 gtggttactg cattggtagt ggcatccaaa gctttttgtg cggcggcttt ggaaatgtcg
    21001 gcttcttgag caatcgcttc gatcaattca gacttgttca caatcagtcc cttcctgtct
    21061 taaaaaatga tgaaatgccc gaatactcgg ggtttgtact gcttgagcaa ctttcgcttt
    21121 atagcaattc tgaaattgcc gtgtcaagca aaaaatacgg aatcacccta tttgacaggc
    21181 tttcaggacg aaaccgcatt tttacaacac atttcctgcg tttcaatgtt tggttgccct
    21241 gctgcggggt tttggttttg aagcggattc cgccgccgct tccgcaccag aaggttctgc
    21301 ccaaggctca ggctggcttt ccaaacccag agccaatacc tcgtctatcc atttgaccgg
    21361 atggatggtc aggccggttt tcacgttttc agggatttct tccaagtctt tgaggttgtc
    21421 ttttggaatt aaaacgtgtt tgatgccgcc gcgaagcgca gccaacagtt tttccttcag
    21481 accgccaatc ggcaggactt cgccgcgcag ggtaatttcg cccgtcatcg ccacatcggc
    21541 gcgcaccggg attttggtga atgcagacac catcgccaaa gtcatggcaa tgcccgcact
    21601 cggaccgtct ttcggcgtcg caccttcggg aacatggaca tggatgtctt ttttctcgta
    21661 aaaatcagga gccaagccca ctgattccgc gcgggagcgg acaaccgacc acgctgcaga
    21721 cacggattcc ttcatcacat cgcccaactg gccggtgcac tgaatcacgc ccttacccag
    21781 caatgctgcg gcttcgacgg tcagcaattc gccgccgact tccgtccacg ccaaaccggt
    21841 tacttgtccg atacggtttt cgctttcggc aacgccgtaa tcgaagcggc gcacacccaa
    21901 atagtcgtgc agatttttct catttacttt aaccgccctg ggcttggctt ttccggtttt
    21961 tttggttttt gagtcttcgt ccaaggtaat ctgcatcacc accttgcggc agattttggc
    22021 aatctcgcgg tcgagcgaac gcacgcccgc ctcgcgggtg tagtaacgga taatatcgcg
    22081 caccgcgctt tcttcgattg ccaattcccc ttcttttacg ccgttgcgct tcatttgctt
    22141 cggcacgagg tactgcatcg cgatattgat tttttcgtct tcggtatagc cggacagacg
    22201 gatgatttcc atacggtcga gcagcggtgt cgggatattc aggctgtttg aagtcgcaat
    22261 aaacatcaca tcgctcaaat cgtaatccac ttcggcataa tgatcggcga acttgttgtt
    22321 ttgctcggga tcaagcactt cgagcaacgc gctggcggga tcgccccgga agtcgctacc
    22381 caatttgtcg atttcgtcga gcaggaacaa tgggtttttt acgccggctt ttgccatatt
    22441 ctgcaaaatc ttaccgggca tggagccgat ataggtgcgg cggtgtcccc ggatttcgct
    22501 ttcgtcgcgc acgccgccca aagccatgcg gacatatttc cgccccgttg ctttggcgat
    22561 ggattcgccc aaagaggttt tgcccacgcc tggagggccg accaggcaca gaatcgggcc
    22621 tttgagtttg tccatacgtt tttggacggc gaggtattcc aaaatccgtt ctttgacttt
    22681 ttccaggccg tagtggtcgg tatccagcac caatccggct ttggcgatgt ctttgctgac
    22741 gcgggatttt ttcttccacg gcagttcgag cagggtgtcg atgtagttgc gtacgacggt
    22801 ggattcggca gacatcggcg gcatcatttt gagttttttc agttcggaca ggcatttttc
    22861 ttccgcttct ttgctcatcc ccgccttttt gatgcctgct tccaaggcat ccagttcgcc
    22921 gttttcgtct tcttcgccca attctttgtg tatcgcttta atctgttcgt tcagataata
    22981 ttcgcgctgg gatttttcca tttggcgttt gacgcgtccg cgtatgcgtt tttcggcctg
    23041 cataatgtcg agttcggatt ccagctgtgc cagcaggaat tccatccgtt tgccgatttc
    23101 gggaatttcc aaaatctgtt ggcgttgcgc cagtttcaac tgcaaatgcg ctgcgaccgt
    23161 atcggttagc cggctgtttt cggcaatgcc gttaatgctg ccgataattt cggcggggat
    23221 ttttttattg agtttggcgt attgttcaaa ctgcgccaac agggtgcggc gcacggcttc
    23281 gaggtcggta ttgccgcccg tgtcttcttc cacgaccgtc tctatatggg aaacgaacag
    23341 accgcccgtg tcttcaatgg tcagaacacg tccgcgatac agcccttcga ccaatacttt
    23401 taccgtgccg tcgggcagtt tcaacacttg caggacttgt gcgaccgtac cggtctgata
    23461 caggtcggcg gcaaccggtt cttctaccgc cgcatcggtt tgcgccaaca ggaaaaccgg
    23521 ctcctcgcgg gtaatggcgt tttccagtgc ggcgatggat ttcggcctgc cgacaaacag
    23581 cggcagaacc atatgcgggt aaacgacgac atcccgcaaa ggaagggttg ccaaggcggc
    23641 atattcctca aaatgctttt ctttttgtgt cataggtact ctcttgtgtc tgacagattg
    23701 ccgattttcg cgtacattgg ggttgaaggt attatttcaa gcatatgtgg tttatttatg
    23761 gagtttgatg cgatgccgtc tgaaacattc cggcttcaga cggcatgggc ttggaaagac
    23821 aaggcgggaa caaaaaactg ttctgtgttg ccgctccttg ctgtaccatc cgtatggttt
    23881 gcggttctgc cgccctattt tcaaaacatg acgcaggtat tccatgtctt cttttatttt
    23941 tcccgataac gggttcgaat ggcgcaacga aagtctgcaa aagccgccca aaggttggca
    24001 ttatgtccgc gaaagtggtg caaacggcat tttgaaggct gcctatcaaa atattgcaac
    24061 tgtctggcag ggcgatttcc acaatgccaa acaggtgctt tccgcaatga agaagaaggt
    24121 ccgcaaacct gccgccgtcc gttccgatgc ggatattgcc gcccttttcc acgcccaccg
    24181 tatgaagcag gcgcagcaga gccgtattct gaatatgctt gccgttgaaa tccgccccgg
    24241 ttttgtgttg gacaacaaac gcgcgcccga tatacgcgcc gccctgctcg acgtgtacgg
    24301 agaggcggac ggcaaaccgt ttttcctgcc gctcaatctg ctgctggggt ttatgggtgc
    24361 gcacgagtgg cataagaaag gggttgccgt tccgcagctg ggcggcagca tacacgttcc
    24421 tttcggcgta ttctcgccgt tgcgcggcga atacctcgac ctgctcgccc atgcgccgtc
    24481 aacgggtttt cagacggcat tcgatatcgg gacgggctcg ggcgtgcttg ccgccatttt
    24541 ggcgaaacag ggcattcctt ccgtcatcgg cacggatacc aatccgaaag ccgtcgcctg
    24601 cgcccgtgcc aatattgccc gtttgggctt tgaaaaacag gttgagatac gggaaaccga
    24661 tctgtttccc gaagggtttg ccgacctgat tgtctgcaat ccgccctggc ttcctgccaa
    24721 gccaacttcc gccgtcgaat ccgcgttata cgaccccgaa tctgcgatgc tggctgcgtt
    24781 tttgcgggat gcgccgaaac atctgaatcc cgatggagaa atccgtctga ttatttccga
    24841 ccttgccgaa catctgcacc tgcgtccgtc cgattttctg gataaggcat ttgctcaggc
    24901 agatttgcgc gttgcggatg ttttgaaaac caaaccggtt cacaaaaaag ctgccgaccc
    24961 ggacgatccg ctggcttttg cccgaaacag ggaaactact ttcctatacc gtttgaaaaa
    25021 ggcataaggg gctgcggtgc gcttccggcg gcaggcatcg gaacgcccgc gccgccggca
    25081 cggcatcatt cgggcggatt attcttgtga aaatacccgc tcgagcatac tgcccaatgc
    25141 cgtctgacgc gttttgacgg tggcggcagc ttgccggaag gcttcttccc cgccgaagaa
    25201 tacgaacttc tctctcgcgc gggtaatggc ggtatataac agctccttac tcaatccgga
    25261 caatgcatcg tccccttcgt ccgaaggtgc ggcggaaggc ggcagcagcc agacttcccg
    25321 gtattccgaa ccttggcttt tgtggacggt catggcgaat gcgggttcaa attcgggcag
    25381 gcagcttacc gctacctttt taaatccgtc cgcatcggca aaataggcgg caaggctgcc
    25441 ctgccgtccg acatcttcca taatcagtcc gatgtcgccg ttgaacagtt caagcgcgta
    25501 gtcgttctgc ctgatcataa tcggctctcc ggcaaaatat gccaaatgtt ccggtatgtt
    25561 cattttgcgg cgtacatggc ggcaataggc ttcgttgaag tcttccgcat cctgccgcca
    25621 agctgccaga accacgatat ccgaaatgcc cgcgtatgcg gcttcgatat tgccgtcttt
    25681 taccgcctgc caataggctt tgtgtgcccg gtacaacctt tcgactcgag cgttcggact
    25741 gcattccgaa tgttccagtt cgtccggaaa ccggtcaaac aatgcccacg ccccttcatc
    25801 gcccgatacg gcggcacggg caaggcagcc gatgccgctg ttgtcgccga agcggtggct
    25861 gaacgacaga tgggcggtgt tttgcgccaa cacgggtgga tttgcgctga cgctgaaacc
    25921 gtgttccgga aggaagccgg ccagcctttg gtgcgtttct ccgtccaaaa cggttttttg
    25981 tgacaaaacg gacagcaccg cccctattcc gacggacggg agctggtttt catcccccag
    26041 cagaatcacg cgcgcgccgg ttttgaccgc ttttaaaagt tgcagcatca atgccgtatc
    26101 cagcatagag gcttcatcga taaccaatac gtcaaacggc agcgggtaaa cagggttgaa
    26161 cgccgcctgc attttgggcg ggcgcagctt cagcagtcgg tggacggttt gcccttccag
    26221 tttgagcaaa tggcggcgga cggcctccgg cgcgtcaaaa ccgttgattg cacggtgcag
    26281 cgcgcgcgcc atatgtgccg ccgctttgcc cgtcggtgcg gcaagcgcga tgttgggaag
    26341 attttcgtct tcaccgcaaa tcagcgccag cagtttggca accgtcgtcg ttttacccgt
    26401 cccgggtccg ccggtaatca ccataaaagg ctgcaacagt gccaaggcgg cggcatcgcg
    26461 ctgcccttcg ctgcccgtgc cttgaaacca ttttgcgagg ttttgccgcg cgcctgccgc
    26521 gtcgggggcg gacgtgtcgg ctgccgccaa gcgttttatc tcggcagcca aatcgtattc
    26581 caactgccac atcctgccca aaaacagcct tctgccttcc aaaatcaaag gcgcggcgga
    26641 tgttccgacc acgggtgcga gtgccgacag cgcgtcagcc tcgccaccgc tcaaacggat
    26701 aaacgaatga ccgttttgca atgcctgaaa caggcgttcg gtgcagtttg caagcacttc
    26761 gctgcctgaa cccgcatagc gtgccaaaaa ccagattgcc gcccttgccg ccgcttgggc
    26821 aaattcatct gtctgccgtt ccattgtatt ccttgtcgaa atgccgtctg aaggcgtggg
    26881 gcttcagacg gcgcggtgtt gtttcggctg tttaggcgtt tgcgccctgt ttggggtgca
    26941 ggctgccgtc tttcatgacc atcacgcgct cgaagcggcc ggcgagttcg tcgtcgtgcg
    27001 ttacgaccac cagccccgtt cccaattccg ttttcagttc cagcatcata tccaaaacat
    27061 tcctggcgtt cgcacggtcg aggttgccgg tcggttcgtc ggcaagcagg cattttggtt
    27121 gcgtaaccaa ggcgcgcgca atggcggcac gctggcgttc gccgccggac agttcgcccg
    27181 cgcggtgcgt cgaacggtgt ttcagtccga ccttttccag catcgccatt gccgtttctg
    27241 ctgcctcttc acggcttttt ttgccgatca gaagcggcat catcacgttt tccagtgcgg
    27301 aaaactccgg cagaagatgg tggaactggt acacgaaacc gagatggcgg ttgcgcagga
    27361 tgcccagttg tcgctggctt aatgtacgca aatcctcgcc catcagcagc aacctgcctt
    27421 cagacggcat atccagcccg cccagaatat gcagcagcgt cgattttccg ctgcccgaag
    27481 aaccgatgat gccggtgctt tcccccgcgc ggatttccaa atccaagccg tgcagcaccc
    27541 gaacgtccaa accgccgtcg cggtagcgtt tgcccacgcc ttcgcatttc aaaatcaact
    27601 cactcataac gcaaagcctc cgccggttgg gtttttgagg cacgccagct cgggtagagc
    27661 gtggcaacga aagacagtcc caaggaaatg caggcaatca gggcaacgtc gcccatatcg
    27721 acatcgctgg gcaggtagtc gataaaataa acctgcgaat tgataaggtg gacaccgagc
    27781 aggttttcaa aaaacgccac gaccctgccg acgttccaac ccaaaagcac gccgcagacc
    27841 acacccgcca gcgtgccgaa aaagcctgaa aacgcgccct gcaccataaa aatcttcatc
    27901 acgccgccag gggaaagacc caaagtccgc aaaatcgcaa tgtccgcctg cttttccgta
    27961 accgccatca ccaaggaaga gacaaggttg aacgccgcca cggcgataat cagcgtcagg
    28021 atgatgaaca tcatccgttt ttccaactcg accgcctcaa aatagctgcg gttgttgtac
    28081 gtccaatcgc gcacccaaac cgcgtccctt tgcgcctccg gaatcagtgt tgccgtcaag
    28141 gcgggggcgt tttgcgggtc ggcgagtttc agccgcagcc ccgcaacttc cttatccaag
    28201 cggtacagta cgcgcgcgtc ttggatatgc gtcatcgcca atgaattgtc aacctcgtaa
    28261 acacccgttt taaccagacc gaccacggta aactgtttca acctcggtac gactccggca
    28321 ggggtaacgt tgccctccgg cgtgatgacg gtaactttat tgccgacttc cgcccccaaa
    28381 gcctccgcca agccgacacc gaggataatg tcaaactcgc ccggaatcag atcttcaaat
    28441 ttgcctgccg gcattttgtc gccgtattcc accactttgc gctcttcaga cggcaaaatg
    28501 ccgcgcatct gcacgcccct gatttcgccc gcgtttgcca gcagagcctg attggaaaca
    28561 tagggcgcgg cagccaaaat acctttgcgg ttttcggtaa accgaagcag gttgcgccaa
    28621 tccgtatccg tattgtcgat atagccgatt tcggcgtgcg gcgcgacatt caggagctgc
    28681 ccgcgtattt ctttctgaaa gccgttcata accgacaaga cgacaatcag cgcggttacg
    28741 cccaaggcga ttccggcaat cgaaaccatc gtgataaacg acataaagcc gttgcgcttt
    28801 ttcgccctga gatacctcaa gcctatccaa gcctctagag aaaacataac gctaccttaa
    28861 aaatgtctgc aaacgtgccg ccccggacgg cggtttgggg gcggcggcaa aagtttattg
    28921 taccgtaaaa cccaggcagc gtccgaacgc ccgagtgcgg gggacggggc ggcagagcgg
    28981 gcggaaaact tgcacaacgc gtcaaactgc cctatccttt ccttcagaaa aaccgtttct
    29041 tgaggaaaac aatgaatatc cgaactgctt ttgctttgtg cgccatcgcc ttatccgccg
    29101 ctgcggctgc ctacgccaaa gaaatcaaaa tcgatgccaa caacacgcct tattccgaag
    29161 ccgacgcgca aaagctggcg gcaacggcag tcggtatggg tgttaaggaa cctatcagcc
    29221 tgaacggcgg cagcgacagc attaccgtgt ccggcagcag cgcgacgcag tgcgtgttca
    29281 aagtcggcaa cggaggcgca ttgcagattc aagggctaaa ctgcaagtaa accgcccgga
    29341 aaaatgccgt ctgaaggctt cagacggcat tttgcattgg cggcgttatg ccccgccttc
    29401 tttaatcagg cggcgttcgt acaccgcctg cgccagcgtt cccgcatcga catattccaa
    29461 ttcgccgccc aagggaatgc cctgcgacag cctgctgact ttgtaaggca ggtttttaaa
    29521 aaactcggac aggacatacg ccgtcgcatt gccttctgcg gtaaaagcgg ttgcaataat
    29581 gatttcttcg acttccccgc cgcccagccg ttgcgccagc ctgtccaatg cgatggcgga
    29641 tacgtccatt cccaatgccg tattgatttg ccccatcagg acgaaataca gcccgtcgtg
    29701 gcagtttgcc gcttccatat tcgacacgtc ggcaggcata tgcaccacca tcagccgccg
    29761 cccgtcgcgt gtttcatcgg cacaaatatc gcacaatccg ccttcgcaaa acgtgttgca
    29821 catcgcgcaa tggtaaacct gcttcaatgc cgtctgcaag gcatccacca gtttttcagc
    29881 ctctttgcgc ttgtgttgga gcaaatgata cgctatccgc tgtgccgatt tcggcccgac
    29941 gttgggtaaa accttcagcg cgtcgatcaa tccttggaag gcatcttgtt ttttgtggct
    30001 catcatattc cgccgtatgg gaaaacggcc ggaatattcc gaccgttatt ttgtcaacaa
    30061 aagtgtcaat tactgaccgt cgccgttgtc gaccgattgc gctcctttgg tctgtttgat
    30121 tttgccgttg aaataacgta tcaacaagtc gaaagtattg gcagactgct gttgcgccaa
    30181 agcctgtttt gcaagcggaa gctgtgcggc gatatcatcc ggcggggtta cagcctgtac
    30241 ttcgacaatc acgggtgccg gcagaccgat cagcctgacg taggcgggtt tgccgtttgc
    30301 cggttttgct ttcagcagtt ccgcataagc ctcgggcggc atggactgcc ttgcctgctg
    30361 tgcgcccaaa acggacactt ccgaccattt cacgtcaaca gccttgccgc cgttcagttg
    30421 ggtaagcacg tcttttgcct tgttttcggc aagtttggcg gcttcggtac ggatataagc
    30481 ctgacgtacc gcgtctttgg cttcggcaaa cggcagggtt ttctcttcgc ggacttcttt
    30541 ggcgcggacg acccacgcgg tttcgctgtt gatggtcagc acttcggaat tgtgtttttt
    30601 cttcaatacg tcgtcgctga aaacggcatt gatcaggttt tcgggcatac cggacatttg
    30661 cgcatcctgc ctgctcagcc aagtttcttg ggtttcgact ttcaaaccgc tgtttttggc
    30721 ggcttcggca agcgaggaag gatggttgaa cgcgtcatcg cccagctttt cttttgcctt
    30781 attgaagtcg gcaaccgcct ttttcatttt caattcgttt tctacggcgg ctttttcctg
    30841 ctcgaaagaa ggtttggctt catttgccgg caaacgcgcc acgcgctctt caaacgcatt
    30901 tttcacttct gtttcgctga cggtctgttt gtctgcaaag tctttcagat tcaaggcgac
    30961 atattccaat ttgaccgctt tgggaagcag gtagtctttt ttgtttgcgt tataaaactt
    31021 ctgcaaatcg gcttcagaca ctttgacttg ggcgatgaat tcgtcggggt tgaaagtgtg
    31081 cgaacggatg gtgcggttga cctgcgtcag cctgatcagc tgttccgcct gcgcgtcgcc
    31141 gaccaatacg ccgttttgga cgaggtttac caaattctgc aaggcaaact gatcgcggat
    31201 ttcttcgaca aactggtctt cagacatatg acgttgggaa aggtagcggt ttaaaagcgc
    31261 gtggtcgaat ttgccgtttg cgtcgtggaa attgggatcg tccacgataa tctgcttgat
    31321 ttgttcggaa gaaaccgaaa tgcccatcag cttcgcgccc tgtttcaggt aggcgcgttg
    31381 cagcagggat tggaacaccg cgtcgcgcga agggctgccg ccgtccgcct gttcattttg
    31441 catagcgttg ttgatggagt ggtcgctgat tttttcgtcg cccacttgga cgatgtagtc
    31501 ggcacccgga tgggataccg tgctgacccc gaagccgacg aaggttaatg caatcagccc
    31561 caaaaggact tgggcgggcg ttctgtattt ttcgatggaa tggaacatat tttcagattg
    31621 gaaatgggat tgggaaattc aagtcgggta ttgtaacggt ttttatccct gtctgcacgg
    31681 ggcttgccgc ttgaagatgc cgtcgtaggt ttcttcgctg aagccgacat aaaccctgcc
    31741 gccgcactcc aatacgggac gcttgatcag gctcggcatt tcggacatca gtttgacggc
    31801 ctccgccgtc gaggacagca ctttttgctg tgttccggca tcaagtttgc gccagctcgt
    31861 cccgcgtttg ttgagcaggg ttgccaaagg cacttgttcc agccacgagc agatttccgc
    31921 ttcagacggc ctctgttttt taaaatcccg aaattcaaac tccaagccgt atccggcaag
    31981 cctgtttttg gcttttttga ctgtgtcgca atttggaatg ccgtgaagga ttatcatttg
    32041 gaaacctttt gtctgaaata ataaaacgga tattttacta taagtgtctg aaaatttgcc
    32101 cgtctgtttc agacggcggg gcggttatgt tacaatccga aaattcgaaa aatttaatct
    32161 cttgttcaat aaaggcttta ccaatcatga tttctaccaa cggcatcacc atgcagttcg
    32221 gcgcgaagcc gctgtttgaa aacgtatccg taaaattcgg agaaggcaac cgttacggcc
    32281 tgatcggcgc gaacggttca ggcaaatcca ccttcatgaa aatcctcggc ggcgatttgg
    32341 aacagacggc cggcgaagtg gcgattgaaa acggcgtgcg tttgggtaaa ttgcgccaag
    32401 accagtttgc ctacgaagac atgcgcgtgc tggacgtggt gatgatgggg cataccgaaa
    32461 tgtgggcggc gatgaccgaa cgtgatgcga tttacgccaa tcccgaagcc accgaagacg
    32521 actacatgaa agccgccgaa ctggaagcca agttcgccga atacgacggc tacaccgccg
    32581 aagcgcgtgc cgccgaactg ttgagcggcg tgggcatttc cgaagatttg cacaatgcga
    32641 aaatggcgga agtcgccccg ggcttcaaac tgcgcgtatt gctggcgcag gcgctgttct
    32701 ccaagccgga tgtattgctc ttggacgaac cgaccaataa cttggacatt aataccatcc
    32761 gctggttgga aggcgtgttg aaccaatacg actccacgat gattatcatc agccacgacc
    32821 gccacttttt gaacgaagtc tgcacgcata tggcggattt ggactacaac accatcacca
    32881 tctatccggg caactacgac gactacatgc tcgcctccgc ccaatcgcgc gaacgcgccc
    32941 tgaaagacaa tgccaaagcc aaagagaaac tgcaagagct gcaagagttc gtcgcccgct
    33001 tctctgccaa caaatccaaa gcccgtcagg caaccagccg tctgaaacag gccgacaaaa
    33061 tcaaatcgga gatggtcgaa gtcaaacctt ccacccgtca aaacccgtat atccgttttg
    33121 aagccgatga aaaagccaag ctgcaccgtc aggctgtgga agttgaaaaa ctggcgaaac
    33181 tctttgaaac ccagttgttt aaaaacctga acttcatcct tgaagcggga caacgcctcg
    33241 ccatcatcgg cccgaacggc gcgggcaaat ccaccctgct gaaactcttg gccggcgcgt
    33301 acaaccccga atattcagac ggcctgttac cggacgaagg caccatcaaa tgggcggaaa
    33361 aagccagtgt cggctactat ccgcaagacc atgaaaacga cttcgacgtc gatatggacc
    33421 tgagcgaatg gatgcgccaa tgggggcagg aaggcgacga cgaacaagtc atccgcggca
    33481 ctttggggcg tttgctcttc ggcagtaacg atgtcgtgaa aaaagtgaag gttctctccg
    33541 gtggtgaaaa aggccgtatg ctttacggca aactgttgct gttgaaaccc aatgtcttag
    33601 tcatggacga accgaccaac catatggaca tggaaagcat cgaatccttg aacatggcac
    33661 tggaaaaata caacggcacg ctgatttttg tctcccacga ccgtcagttc gtttcctcct
    33721 tggcaaccca aatcatcgaa ctggacggca aaggcggata tgaacactac ttgggcgatt
    33781 acgaaagtta cttggagaaa aaaggcgtag cataaccgcc ggttggaaca atgccgtctg
    33841 aagccgcttc agacggcatt gttgataact ttaaaatagg aagcatatgc agacttatct
    33901 cgtcggcggt gccgtccgcg attatctttt gggcttgccc gtcaaagacc gcgattgggt
    33961 ggtcgtcggc gcagacgcac aaaccatgct ggcgcaaggc ttccagcctg tcggcaaaga
    34021 ttttcccgtg tttctccatc ccgaaacgca cgaagaatac gccctcgccc gcaccgagcg
    34081 caaaaccgcc aaaggttacg tcggtttcag tttccacgcc gacaaagacg ttacgctgga
    34141 gcaggatttg atgcgccgcg acctgaccat caacgcgatg gcgcaagatg cggacggcaa
    34201 gattatcgac cctttcggcg gacaacggga tttggcggca ggcattttgc gccacgtttc
    34261 cccggccttc gccgaagacc ccgtccgcat cctgcgtact gcccgctttg ccgcgcgtta
    34321 caggtttgaa atcgccgaag aaaccataaa gctgatgcgg cagatggtgg aaaacggcga
    34381 agcggacgca ttggttgccg aacgcgtctg gcaggagttt gcgaaaggtt tgatggaaaa
    34441 aaatccgcgc aaaatgattg aagtgttgcg cgaatgcggc gcgctcaaag tcttgctgcc
    34501 cgaagtcaat gccctcttcg gcgtgccgca acgcgccgac taccatcccg aaatcgacag
    34561 cggcatccat accctgatga cgctgcaacg cgccgccgat atgggtttga gcctgcccga
    34621 acgctatgcc gccctgctgc acgacttggg caaagccaaa acaccgtccg acatcctgcc
    34681 gcgccaccac ggacacgacc tcgccggtgt cgaacccgtg cgcgaagtca atcagcggct
    34741 gcgtgcgccg aaacattgcg ccgagcttgc cgaacttgtc tgccgttggc acattatttt
    34801 ccaccaagtc ggacagctta aaagccaaac cattctgaac gttttgaaaa aaaccgacgc
    34861 tttcagacgc cccgaacgct tctccgctgc cttaaatgtc tgcattgccg atacgcaagg
    34921 tcgtctgaac cgcgaacaca cgccctaccc gcaacgcgcg cactggctcg ccttactcga
    34981 agtcgccaat caagtggatt cgggcaaaat cgccgccgaa tgccgcgcgc aggggaaagt
    35041 gcaccttatc gccgaacaaa tcgaccgggc gcggctggca caaatcgccc cattgcaaaa
    35101 agcgtttcga gcggcgcaag acaaaacaga aaaacattaa aacgcccaat gcagcc
//
LOCUS       DAWFAN010000004        32176 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWFAN010000004 DAWFAN010000000
VERSION     DAWFAN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.004"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(379..654)
                     /locus_tag="WGO65_000134"
     CDS             complement(379..654)
                     /locus_tag="WGO65_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0326501.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(651..1934)
                     /locus_tag="WGO65_000135"
     CDS             complement(651..1934)
                     /locus_tag="WGO65_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0326502.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWPEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWIFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2090..3508)
                     /gene="glnA"
                     /locus_tag="WGO65_000136"
     CDS             complement(2090..3508)
                     /gene="glnA"
                     /locus_tag="WGO65_000136"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ0326503.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDAGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3592..3741
                     /locus_tag="WGO65_000137"
     CDS             3592..3741
                     /locus_tag="WGO65_000137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326504.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLLFVCSKAEYPPFCPNAD
                     KTDAV"
     gene            complement(3782..4168)
                     /locus_tag="WGO65_000138"
     CDS             complement(3782..4168)
                     /locus_tag="WGO65_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326505.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVQVGIDSDNNTTFGDADSRTD
                     NRAWFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPS
                     YAEWNYVIPGTLGEAQYKFVCNGYPR"
     gene            4339..5148
                     /gene="aroE"
                     /locus_tag="WGO65_000139"
     CDS             4339..5148
                     /gene="aroE"
                     /locus_tag="WGO65_000139"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ0326506.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGIGLTNDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            5150..5851
                     /gene="mtgA"
                     /locus_tag="WGO65_000140"
     CDS             5150..5851
                     /gene="mtgA"
                     /locus_tag="WGO65_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ0326507.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDAHFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(6054..6129)
                     /locus_tag="WGO65_000141"
     tRNA            complement(6054..6129)
                     /locus_tag="WGO65_000141"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6094..6096),aa:Phe,seq:gaa)
     gene            complement(6320..7054)
                     /gene="lptB"
                     /locus_tag="WGO65_000142"
     CDS             complement(6320..7054)
                     /gene="lptB"
                     /locus_tag="WGO65_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ0326508.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(7128..7658)
                     /gene="lptA"
                     /locus_tag="WGO65_000143"
     CDS             complement(7128..7658)
                     /gene="lptA"
                     /locus_tag="WGO65_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ0326509.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7639..8220)
                     /gene="lptC"
                     /locus_tag="WGO65_000144"
     CDS             complement(7639..8220)
                     /gene="lptC"
                     /locus_tag="WGO65_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ0326510.1"
                     /translation="MKVRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPNEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDLPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(8217..8753)
                     /locus_tag="WGO65_000145"
     CDS             complement(8217..8753)
                     /locus_tag="WGO65_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0326511.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLSVAVLDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMRAKGTLGAALNEYIK"
     gene            complement(8818..9792)
                     /locus_tag="WGO65_000146"
     CDS             complement(8818..9792)
                     /locus_tag="WGO65_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ0326512.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            9888..10943
                     /gene="tal"
                     /locus_tag="WGO65_000147"
     CDS             9888..10943
                     /gene="tal"
                     /locus_tag="WGO65_000147"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ0326513.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRASCGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALEAQVANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            11097..11387
                     /locus_tag="WGO65_000148"
     CDS             11097..11387
                     /locus_tag="WGO65_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326514.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            11404..12291
                     /gene="gluQRS"
                     /locus_tag="WGO65_000149"
     CDS             11404..12291
                     /gene="gluQRS"
                     /locus_tag="WGO65_000149"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ0326515.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQTGARRGADGFVYNGRCRNPQQRPAPQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMGKVPKHAITTP"
     gene            complement(12361..13371)
                     /gene="dusA"
                     /locus_tag="WGO65_000150"
     CDS             complement(12361..13371)
                     /gene="dusA"
                     /locus_tag="WGO65_000150"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ0326516.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQCLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(13402..13893)
                     /locus_tag="WGO65_000151"
     CDS             complement(13402..13893)
                     /locus_tag="WGO65_000151"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ0326517.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(13966..14724)
                     /locus_tag="WGO65_000152"
     CDS             complement(13966..14724)
                     /locus_tag="WGO65_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0326518.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSEDELHKFYEQQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(14795..15661)
                     /locus_tag="WGO65_000153"
     CDS             complement(14795..15661)
                     /locus_tag="WGO65_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0326519.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(15710..15988)
                     /locus_tag="WGO65_000154"
     CDS             complement(15710..15988)
                     /locus_tag="WGO65_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0326520.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGDMIHALGIRAATPDEYFHTAD"
     gene            complement(15988..16278)
                     /locus_tag="WGO65_000155"
     CDS             complement(15988..16278)
                     /locus_tag="WGO65_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ0326521.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(16281..16811)
                     /locus_tag="WGO65_000156"
     CDS             complement(16281..16811)
                     /locus_tag="WGO65_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ0326522.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVIFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            17119..19644
                     /locus_tag="WGO65_000157"
     CDS             17119..19644
                     /locus_tag="WGO65_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ0326523.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENVTETV
                     AETPDFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTKTVA
                     EIPDFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENAAEITLE
                     TPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAPSEAKY
                     DLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(19817..20293)
                     /gene="gloA"
                     /locus_tag="WGO65_000158"
     CDS             complement(19817..20293)
                     /gene="gloA"
                     /locus_tag="WGO65_000158"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ0326524.1"
                     /translation="MADVYAKQNRSLTTTERNTKMRLLHTMLRVGNLEKSLDFYQNVL
                     GMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDA
                     YEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(20257..21426)
                     /gene="lapB"
                     /locus_tag="WGO65_000159"
     CDS             complement(20257..21426)
                     /gene="lapB"
                     /locus_tag="WGO65_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ0326525.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(21437..21751)
                     /locus_tag="WGO65_000160"
     CDS             complement(21437..21751)
                     /locus_tag="WGO65_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ0326526.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            22061..23059
                     /gene="ilvE"
                     /locus_tag="WGO65_000161"
     CDS             22061..23059
                     /gene="ilvE"
                     /locus_tag="WGO65_000161"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ0326527.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLVPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(23244..24029)
                     /gene="fabI"
                     /locus_tag="WGO65_000162"
     CDS             complement(23244..24029)
                     /gene="fabI"
                     /locus_tag="WGO65_000162"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ0326528.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELGSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(24184..25005)
                     /gene="dapD"
                     /locus_tag="WGO65_000163"
     CDS             complement(24184..25005)
                     /gene="dapD"
                     /locus_tag="WGO65_000163"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ0326529.1"
                     /translation="MSLQNIIETAFENRADITPSTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(25015..25245)
                     /locus_tag="WGO65_000164"
     CDS             complement(25015..25245)
                     /locus_tag="WGO65_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326530.1"
                     /translation="MPSERRRRGIKVLPPFCIDSGAEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILLENTGKRPTKHLT"
     gene            complement(25215..26858)
                     /gene="pgi"
                     /locus_tag="WGO65_000165"
     CDS             complement(25215..26858)
                     /gene="pgi"
                     /locus_tag="WGO65_000165"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0326531.1"
                     /translation="MNAFTRAWHALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNNSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEEGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGTIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            27368..28600
                     /gene="pilG"
                     /locus_tag="WGO65_000166"
     CDS             27368..28600
                     /gene="pilG"
                     /locus_tag="WGO65_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ0326532.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            28674..29534
                     /locus_tag="WGO65_000167"
     CDS             28674..29534
                     /locus_tag="WGO65_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ0326533.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLIAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLL
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGETGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            29536..30168
                     /locus_tag="WGO65_000168"
     CDS             29536..30168
                     /locus_tag="WGO65_000168"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ0326534.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSQKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            30161..30370
                     /locus_tag="WGO65_000169"
     CDS             30161..30370
                     /locus_tag="WGO65_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ0326535.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(30455..32131)
                     /gene="pilB"
                     /locus_tag="WGO65_000170"
     CDS             complement(30455..32131)
                     /gene="pilB"
                     /locus_tag="WGO65_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ0326536.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         7534 a         9080 c         8218 g         7344 t
ORIGIN      
        1 attatggtag atactaactc tttaagttga catattgtgt gatatcgagg gctgtaccgt
       61 attttcccat cgtattttcc catcgtattt tcccatcgta ttttcccgtt ctttatatga
      121 gcggcaggca tcctcataaa agaagggata ccgataagga aaaatacccc agcataaaaa
      181 atatactgat taatcaatac aaatccaaca aacagttcat cgtattcttt tctgttggcg
      241 tataaaaata ctgatatatt ccttcacttt catagggcga cgctttatgt accgtcctgt
      301 gtgttgaaac gtcggttttc cttaccgcct gccccgcttt tccctgattg ctgccaactg
      361 ccgtccgaac ttgaaccgtc aggtttcaga cggcattttt tctgcctgct cccacaattg
      421 ccgtttttgt gcggcggtca gttttttgac ggcgatttcc tgcctgagtg ccgttccttt
      481 atccatcccg cctgcaacga tacgcatcgc caccggtttg aatacgcggg tatatttcgc
      541 gcctttaccg gttgtgtggg cggcaagccg ctgttgcgga ttcgggctga tgccgcaata
      601 gaacgcgctg ttttcacaca atatcaggta aacgctccag tttgacgcgt tcatctgcct
      661 gcatcctgag ttttctcccc gttccaaggc gcaactttca gcagcagcag catacccggt
      721 agggcgagtg cgaaacacag ctggaaaaac ggtacatacc cgagccattc gatcagataa
      781 ccggcaaagg aattgatgac cgtacgggga acagcggaca ggctggtaaa cagcgcaagc
      841 tgcgttgcgg taaatgcggg attggtttca cgcgccatat acgatacgaa cgccgccgtc
      901 cccaaaccca cgccgactgc ctctgcgccg ataactgccg ccagcatcag cctctcgcct
      961 gtgccgaccg tgtcgaaatg tccgaaccct gccagccata caaaccccaa aacggttaca
     1021 gcctgcaccg cgccaaatat ccacaaggct ttgtttacgc cgattttcag catccacacg
     1081 ccgcccaaga tacctgccgc cactgccggc cacagtcctg catttttcgc aatcaaaccg
     1141 atgtctgtct tgctgaaacc catatccaga taaaacggcg ttgccaacgc ggttgccata
     1201 ctgtcgccga gtttgtaaag gaagataaac agcagcacgc acaccgccga agcgatgccc
     1261 ttgcgcgtaa aaaattcttt aaacggctct accacggtct gcttcaacgt tttaggaacg
     1321 gcaggcggca acacgggttc gcgcgcaaga aacagcgtca tcagaaggcc gggcagcata
     1381 aacagcgaag tgatgacaaa cacctccggc cacggcatcc tgtctgccaa caccaaactc
     1441 aatgaaccgg gaatcagggc ggcaatccgg taggcgttca catgaaccga gttgcccaaa
     1501 cccaattctt cgtctgacaa aatctcgcgc ctgaacgcat ccaatacaat atcctgactg
     1561 gcggaaaaaa aagcgacaag caccgacaag ccggcaatca gcggcagatg attacggggg
     1621 tttaaaaagg cataggccgc caaagccgcc agcaaccctg cctgcgtcag cagcatccac
     1681 ccgcgccgcc gtcccaaaac gggcagcctg accgcgtcca tcagcggcga ccacaaaaat
     1741 ttccaagtaa acggcagacc gattaacgcc atcagcccga tgctcttcaa atccacctgc
     1801 tcgctgcgca accacgccgg aatcaggtta atcagaaagt acagcggcag ccccgaggta
     1861 aaccccgtaa aaatacagac aagcatattt cgggaaaaga tttgcccgat aaaacctgat
     1921 ttcgatgcag tcatcgttcg tgcagcccaa aaaaaacaaa agcctattgt agcaaatatc
     1981 gcccgcctgc tgaaaaatgc cgccccgcgc cgccggtttg ccgttcaaat gcaaaatgcc
     2041 gtctgaaact gtgttcagac ggcattgctt ttctgaaaac cagacgtgct tacaggctgt
     2101 aatacatttc aaattccagc gggtgcggcg ccatgcggat gcggcgtacg tcttcttctt
     2161 tgaaagcgat gtagctgtcg atccagtctt tgctgaacac gccgccgcgc aggaggaatt
     2221 cgtggtcggc tttgagggcg gccagtgctt cttccaaaga agcgcaaacg gtcggcacca
     2281 atgcatcttc ttccggcggc agatcgtaca ggtttttatc ggcagggtcg cccggatgga
     2341 ttttgttttg aatgccgtcc aaacccgcca tcaacagggc ggcaaatgcc aaatacgggt
     2401 tggcggtcgg atcggggaaa cgcgcttcga tgcggcgcgc cttgctgctg ttcacggacg
     2461 gaatgcggat ggaagcggaa cggtttttgg cggagtatgc cagtttggtc ggcgcttcaa
     2521 agtgcggcac gaggcgtttg taggagttgg tggacggatt ggtaatcgcg ttcaaggctt
     2581 tggcgtgttt gatgatgccg ccgatgtagt agagcgcggt gtcgctcaag ccggcatagc
     2641 cgtcgcctgc gaacaggttt tgaccgtctt tccaaatgga ttggtgaacg tgcataccgc
     2701 tgccgttgtc gcccataatc ggtttgggca taaaggtggc tgttttgccg aagttgtggg
     2761 caacgttttg aatcacatat ttcatgtctt gggtttggtc ggcgcgtttg accaaggtgg
     2821 cgaaacgcgt gccgatttcc atttggctgc cggtaccgac ttcactgtgg tggacttcga
     2881 cttcgatgcc gagttcttcc aaaatgttta ccatcgcgga gcgcaggtct tgtcccgcgt
     2941 caatcggagc aacgggcgcg taaccgcctt tgacggcagg gcggtggccg gtgttttgac
     3001 cgtccatatg cagaccgctt gcccacgcgc cgctttcgga cgtgatttcg taacgggttt
     3061 tgtgcatatc ggtttcaaat tctatgccgt cgaatacgaa aaactcgggt tcgggaccga
     3121 aataggcggt gtcgccgatg ccggaagatt tcaaataggc ttcggctcgg cgggcgatgg
     3181 agcgcgggtc gcggtcgtaa ccctgaccgt cggcgggatc gataacgtcg caagtcaaca
     3241 caacagtcgc atcatcataa aaaggatcga cgaaggctgt agacgcatcg gggcgcaact
     3301 gcatatcgga agcctgaatg cctttccagc cgccgataga cgaaccgtca aacgcctgac
     3361 cgttttcaaa ccactcttcg gggtcttcca acacgatgcg cgcaggcacg gtaaagtggt
     3421 gctgcttgcc tttggtatcg gtaaagcgca aatcgacaaa gcgggcttcg ctttcttcaa
     3481 tcaattttac ggcgttttta atagacattt ttcagctcct ggaaaaggga aatagcaaaa
     3541 tatgcggaac gcggcggctt gacgcaaagt cggcatgatt ctaccacgtc catgccgtct
     3601 gaaactcgcc ctgcgtcaaa tttcaagctg gaaaatatag tttcagacgg caagattgtc
     3661 aacaatatgc ttctgtttgt ctgttcaaaa gcggaatacc cgcccttttg tccgaatgcc
     3721 gacaaaacag atgccgtctg aaagcagttc agacggcatc tgttgtttgg tttgaaaacg
     3781 gtcagcgagg atagccgttg cagacaaatt tgtactgagc ctctccaagc gtaccgggaa
     3841 taacataatt ccattcggca taggatggtg tataagaagc gatagagttt cccgatttgg
     3901 aataccacgt tgccgtcaat atcctaaaac gtttaccact gcaatccatt tcttccaacg
     3961 ttttagctgt attaaaaaat tttccattgc catacttttg cggcttggcg aaccttgttt
     4021 caaaccatgc tctgttgtcc gtacggctgt ctgcatcacc aaacgtggta ttattgtcac
     4081 tgtcaatacc tacctgcacc caatccgccg ccaccgccgg tgttgccaac cccaatgcaa
     4141 atactgccaa gcacaacatc tttttcatgt ccgaccctct cgtccttgtc aacaaaataa
     4201 agttaaattg taaaaaaaaa cgactttttt caagagaaaa atattccgtc atgctatctg
     4261 acaaccgttt cagacggcat cgtttccatc tgtacgccgc cgttataaaa tagccttccc
     4321 gtcttttgga acacgaccat gaccgccctc ccccgttact ccgtttttgg caaccccgtt
     4381 gcacacagta aatcgccgca aatccatcag caatttgccc ttcaggaagg cgttgacatt
     4441 gaatacgaac gcatttgcgc cgacatcggc ggttttgcac aggcggtttc gacatttttt
     4501 gaaacaggcg gttgcggggc aaacgttacc gtaccgttca aacaggaagc gtttcatctg
     4561 gcggacgagc attccgaacg cgcattggcg gcaggtgcgg tcaatacgct gattccgttg
     4621 aaaaacggca agctgcgtgg cgacaacacc gacggtatcg gtttgaccaa cgacatcacg
     4681 caggtcaaaa atattgccat cgagggcaaa accattttgc ttttgggcgc aggcggcgcg
     4741 gtgcgcggcg tgattccggt tttgaaagaa caccgccctg cccgtatcgt cattgccaac
     4801 cgtacccgcg ccaaagccga ggaattggcg cagcttttcg gcattgaagc cgtcccgatg
     4861 gcggatgtga acggcggttt tgatatcatc atcaacggca cgtccggcgg tctaaacggt
     4921 cagattcccg atattccgcc cgatattttt caaaactgcg cgcttgccta cgatatggtg
     4981 tacggctgcg cggcaaaacc gtttttagat tttgcacgac aatcgggtgc gaaaaaaact
     5041 gccgacggac tgggtatgct agtcggtcaa gcggcggctt cctacgccct ctggcgcgga
     5101 tttacgcccg atatccgccc cgttatcgaa tacatgaaag ccatgtaata tgttccgcat
     5161 cgtcaaatgg ctgattgccc tgcccgtcgg catctttatc tttttcaatg cctatgtgta
     5221 cggcaacatc attacctacc gcgccgtcgc gccccatcgg actgccttta tgtcgatgcg
     5281 gatgaagcag tttgaacagg aaggtcgcga tgtcgcactg gattaccgct gggtgcccta
     5341 caaccgcatc tcggtcaacc tgaaaaaagc cctgattgct tccgaagatg cccattttgc
     5401 cgggcacggc ggcttcgatt ggggcggcat tcaaaacgcc atcaggcgca accggaacag
     5461 cggcaaagtg aaggcgggcg gctcgaccat cagccagcag cttgccaaaa acctgttttt
     5521 aaacgaaagc cgcagctata tccgcaaagg cgaagaagcg gcgattaccg cgatgatgga
     5581 agccgttacc gacaaagaca ggatttttga actgtattta aactcaatcg aatggcacta
     5641 cggcgttttc ggcgcggaag ccgcgtcccg gtatttttat caaatacccg ccgccaagct
     5701 gaccaaacag caggcggcga aactgactgc ccgcgtcccc gccccgctct actacgccga
     5761 ccatccgaaa agcaaacggc tccgcaacaa aaccaatatc gtgctcagac gcatgggttc
     5821 ggcagagttg cctgaaagcg acacggactg agtgccgccg caatataaaa tgccgtctga
     5881 aatatcattc agacggcata tgctttctgc ctttaccctt ttctctaaaa cgcaacgggg
     5941 cggtcattat cgcttttgtc cttctacata acggtcagac acaaaacgtg tccgacacgg
     6001 acaacttccc aaaaacggca ggcaaaaaaa ataaccgctt ataagcggtt ttttggtggc
     6061 cagaggcgga atcgaaccgc cgacacacgg attttcaatc cgttgctcta ccaactgagc
     6121 tatctggcct ttgtctgtct cgttaagaga tgcgtattaa accaaattac gggacgacat
     6181 gcaagcatat cgggcaatat tctttgaaaa caggtttaag tcaatgtttt aaatttgaat
     6241 taatttatgt aaaaacaaca taaatccgta tttttgccgc ctgcccgacg atattaggtc
     6301 gcccgtctga aaaatatttt caatatttga agttcttacc cagataaaca gaacgaacct
     6361 gttcgtttcc gaccaaatca tcaggttttc ccgatgccaa caccgtgccg tctgaaataa
     6421 tgtaggcccg atcgcagatg ctgagggttt cgcgtacgtt gtggtcggta attaatacgc
     6481 cgataccgcg cgacttgaga aatccgatga ttttttggat gtcgatgacg gcgatgggat
     6541 cgacgccggc aaaaggctcg tccaacaaaa taaaatgcgg tttcatggca agtacgcggg
     6601 cgatttcgac gcgtcggcgt tcgccgcccg acaaagatgg ggcaggatta ttgcgcaaac
     6661 gctcgatatt caggtcggca agcagttttt caacttcgcc gtcgatgcgg cttttgtctt
     6721 tggtactgat ttctaaaatg gcgcggatgt tttgttcgac ggtcatttta cggaaaatag
     6781 aggcttcctg cggtaggtag ccgacaccca ggcgggcgcg ttcgtgtatg ggcaggtgac
     6841 gcaattcctg cccgtccagc atcacgctgc ccgcatcggc ggcaatgagt ccaacaatca
     6901 tgtagaagct ggtggtttta cccgcaccgt tgggcccgag caggccgacg acttccccgc
     6961 tttcgatttc aagggagaag cttttaacga cttggcgttt tttgaaactt ttttgcaggt
     7021 tttgaacaac aaggcggctg acgtttgcgc tcataaatat ctcttcaata tcttcggtcg
     7081 gcaaatgccg tccgaaccag gtttatgttt cagacggcat tttgggatta ttcggatttt
     7141 tgcgtactcg aaggctggat aacgacgctg accctgccgg atttggaagc ggattttgcg
     7201 ccggattttg tgctgccgct gatggtatag acttcggttt tggtgttgta tgtaatcacc
     7261 gcaccttcgg cgacatcgcc gccgcgctgt actttggcat taccggttaa gactacggtg
     7321 ctgcctgcag atgaataagc aacgttgttt gcctgtccgc gcaccgtgcc tttgccgccg
     7381 tccaatgtct ggctgaagcg gactggcgaa ccttccgccc tcacggattc gccgcctttg
     7441 ccgccgcgtg taacattgac gcgggcggcg gaaatattga gcgtaccctg tctgatgacg
     7501 acgtttccgc tgaatgtggt gctttggttg gcttgatcga gcgaaccttg gtcggcctca
     7561 atctgaatag gctgcctgct gtcgctttga agggcaaaag cgggggacgc cgaaaaaaat
     7621 gctattaaaa caaatagctt acatatcttt tgtatcataa atcgtggctt tcactttaga
     7681 tgagaagttc aacatgcctg ttttgtggtc gtaagtcatg ccgcccgcct gaccgtgcga
     7741 tgcaccatat tggaaactga caggcgtatc ggtttgggca tattgagatt cggtatcgac
     7801 gtgcagcttt tcggcttcaa ctttacccgc ctgccgtttg ccgtcggcgg ttttggtcag
     7861 cacaacgttg tttttaaaaa gaacctgttt gttttcggta tggtaaacgg cttcatcgct
     7921 gccgacttcg tacaacaacc tgccttcttg gaagaacacg agatgcggta aatcgaaatg
     7981 aatgtcgctg ctttcgggaa actgtttcgc gcctttcgcg ctcaaatgtt ctttcaagta
     8041 tccctgttcg tcaaaccgcc tgccgtccaa gccgtccatt gtgtattggg gttcgttggg
     8101 attgagcctg acttcctcga tttcgacttc gctgatacga cccaaccatg ccgacagacc
     8161 gcccaaggca accgccaata tcaatgggaa cgcaattccg taccgccatc ttactttcat
     8221 ttgatgtact cgttcaaagc cgcgcccagc gtacctttcg cgcgcataat caggtcgcac
     8281 acttcgcgca ccgcgcccgc accgccgggg cgtttggcaa tataggcagc gtgttgcagc
     8341 gtaaaccaat gcgcgtcgag gacggcaacc gacaatccgc aacgcaccat caccggcaaa
     8401 tcgaccacgt cgtcgccgac aaaggcacac tcggcttctt ccacgccgac ctgagcgcgc
     8461 agctcttcat acgcggcgcg tttgtccgaa atgcctttga agtagtaatt tatgcccaac
     8521 tgtttgacgc ggatgccgac ggagggcgcg tcccgacctg tgataatcgc agtctgcacg
     8581 ccgcttgcct gaagcatttt cagaccgtgt ccgtccagtg tgtgaaacga tttgatttct
     8641 tcgccgttat cgcggataaa gatgcgcccg tcggtcaaaa cgccgtccac atccaggatc
     8701 aacagtttga ttttggcggc gcgcgcctgt aattcgggag aaattgcctg catgggattt
     8761 ccttgttttc agacggcata tcttaccaca aatgccgtct gaaacggctg caacgcctta
     8821 tacaatccgt gccgccagca ggtcgtgcat attcagcgcg ccgatcagca cgccatctgc
     8881 atcggtaacc agaagcccgt tcacatggtt tgcctgcatg actttcaggg cttcggtggc
     8941 gagacgttcg gcggagatgg ttttaggatg cgtatgcatg acttcgtcta tcgaaagacc
     9001 ggtaaaattg tcgcattctt gaaacaggcg gcgcaaatcg ccgtcggtga atacgccttt
     9061 cagacggcct tgcccgtccg ttaccgccaa catgcccagc cctttttcac tcatgctgac
     9121 gatggcttct ttcaagggcg tgccgagtcg gacggcaggc aggccgccgc ctttgtgcat
     9181 aatgtcggca acgcgcaaaa gtaggcgttt gccgaggctg ccggcaggat ggctcaaggc
     9241 gaaatcgtcg ggcgtgaacg cgcgtgcgcg cagcaggacg accgccaacg catcgcccaa
     9301 agccatgacg gcggtggtgc tggtggtcgg ggcaagcccc agcgggcagg cttctttgga
     9361 aaccgacgcc gtgatgtgga tgtcggcatg gcgcgccatg gttgaatcgg ggcgggcggt
     9421 gatgcagaca agcgtgatgt ctttgcgttt gagcgcgggg atgatggcgg cgatttcgtc
     9481 gctttcgccg gaattggaaa tcgcgacgac cacgtcgttg tccacaatca tgcccaaatc
     9541 gccgtgtgcc gcttccgcag ggtggacgaa aaacgcgggc gtgccggtcg aggccatggt
     9601 tgccgccatt ttgcgcccga tatgtcccga cttgcccatg cccgtgataa caaccctgcc
     9661 cttgcagtgc aacaacgcgt ctgccgcaag gacgaagttt tcgtccaatt ccgctgcaat
     9721 ttcgcgcaag ccttccgctt cggtgtgcaa cacttcgcgc gcccagtcga gatattttcc
     9781 gttttctgcc attgccatcc gcctttgctg taacaaaact acccaaaatt tccgaatcgg
     9841 ggtagcataa gcattcaaac cccgccatcc atccaaagga atcagaaatg actattttat
     9901 cggacgttaa agcattagga caacaaatct ggctggacaa cctttcccgc tcgctcgtgc
     9961 aaagcggcga attggcgcaa atgctgaaac aaggcgtgtg cggcgtaact tccaatcccg
    10021 ccatcttcca aaaagccttc gccggcgacg cgctttacgc cgatgaaatc gccgccctca
    10081 agcagcaaaa cctcagcccc aaacaacgct acgaaaccat ggcggttgcc gatgtgcgtg
    10141 cctcctgcgg cgtttgcctt gccgaacacg aatccaccgg cggcaaaacc ggtttcgtca
    10201 gcctcgaagt ttcgcccgaa ttgtccaaag acgcgcaagg cacggtagaa gaagcgcgcc
    10261 gcctctacgc cgccatcggg tgcaaaaacg cgatgattaa agtgcctgcc accgacgcag
    10321 gcatcgatgc gctcgaaacc ctcgtttcgg acggcatcag cgtgaacctc acgctgctgt
    10381 tctcacgcgc ccaaaccctc aaagcctacg ccgcctacgc gcgcggcatt gccaaacgct
    10441 tggcagctgg acaaagcgtt gcccatatcc acgtcgtcgc cagcttcttc atctcccgcg
    10501 tggacggcgc gctggacaca acactgcccg accacctcaa aggcaaaatc gccatcgccc
    10561 ttgccaaagc cgcctaccaa gactgggctc aatacttcgg cagcccggaa tttgccgcgc
    10621 tggaagccca agtcgcaaac cgcgtgcagc ttttatgggc atccaccggc gtgaaaaacc
    10681 cggcctatcc cgacacactc tacgttgaca gcctgatcgg cgcgcacacc gtcaacaccg
    10741 ttccagatgc cacgctcaaa gcctttatcg accacggcac agccaaagcc acgctgaccg
    10801 aaggcgtaga agaagcacaa gcgcagcttg ccgaaaccgc cgcgctcggc atcgatgtcg
    10861 aaaccttggc ggcacgtttg caggaagacg gtttgaaaca gtttgaagaa gcgtttgaaa
    10921 aactgctcgc accgttggtt taaacttttc atccccgatg ccgtctgaaa ggctttcaga
    10981 cggcatcgcc cgtttgaccg gacacagtgc ggcatatcaa ggattcaccc taaaaatatt
    11041 aatgggcaag aagttaatgc acgacattac ccaccaacac aaaaggaaat agaatcatgg
    11101 caaaagcact cgaaatcatt tcacccaatg aaatttattc agatctgatt tttaaggatc
    11161 cggtacctcc ccatacaata tatacagaat tgatgaaact ggttagtaga aatgcaagta
    11221 atgagagaat cgcagataga gccttcgatt ttttctcgtc cggccttatg gatggttcag
    11281 ctaccgaaga gcaatataat gccctatatg atttaacctt acttgaagaa cccgggatgg
    11341 aactgaataa agatgagata atggctttga ttaatagtct taaataaagg ggccgtaccg
    11401 gatatgtaca caggacgttt cgcccccagc ccgaccgggc tgctccacat cggctcgctg
    11461 ctgaccgccg ccgcttccta tgccgatgca cgctcaaacg gcggcaaatg gctggtccgc
    11521 atggaagacc tcgatccgcc gcgcgaaatg ccgggggcgg caagccatat cctgcacacg
    11581 cttgaggcat tcggattcga gtgggacgga gaagtcgcct atcagagccg ccgttacgcc
    11641 ctgtatgaag aaacactatg ccgcctgaaa accgccggac tggtctatcc ctgccattgc
    11701 agccgcaaag actggcagac cggggcaagg cggggcgcag acgggttcgt ctataacgga
    11761 cgctgccgca atccgcagca acgccctgca ccgcaaggca aacagcctgc gtggcgcatc
    11821 cgcgtccccg accgcgacat cggcttttca gacggcatcg tcggcggtta cgcccaaaac
    11881 ctcgcccgcg acatcggcga tttcgtcctg cttcgtgcag acggttactg ggcataccag
    11941 ctcgccgtcg ttgccgacga tgccgaacag ggcgttaccc acatcgtccg cggacaagac
    12001 ctgctcgttt ccacgccgcg ccaaatctat ttgcagcagt gtttgggcgt tccgacaccg
    12061 caatatgccc acctgccgct gctgaccaac gcacaagggc agaagtggtc gaaacagacg
    12121 ctcgcccccg cattggattt aaaccgccgc gaacaactcc tccgccaagt gttccgttac
    12181 ctcaagctgc ccgaagcacc ggaaaccgac cgccctgccg aactgctcga ctgggcggtg
    12241 gcacactggg atatgggcaa agtgccgaaa cacgccatta ccacccccta aacaccgccc
    12301 gacaaacgca caaatgccgt ctgaagcctt cagacggcat ttcacacata cagccccgcc
    12361 ctattcgcgc gtatttgccc gctcaacctc tttccacgct tcgagaatca ggctgccgtc
    12421 gttgtctttc aagagcgttg cgtcggaaag catacgccgc caagtccgcg cacctttcaa
    12481 accgtgcatc agcccgaggc tgtggcggac gatgtggcgc aagattgtgc cgcgtccggc
    12541 ttggatctgg gcttggctgt atgtgtagag gcactgcacc aaatcggcgt attcgatcgg
    12601 gctgcgagta tcgccgtaaa acaatctgtc ccattcgcgc atcaccatcg ggttgtggta
    12661 cgcctcgcgc ccgaccatca cgccgtcaac gtgttgcagg tgtccggcga ttgcttcgtt
    12721 ggtggtgatg ccgccgttga tgatgatttc cagcccggga aactcctgct tgaggcggta
    12781 aacgtaatcg tatttcaacg ggggaacgtc gcggttttct ttgggggaaa gaccgtccag
    12841 ccaagcgttg cgggcgtgga cgatgaaggt tttgcaggcg gttttgtcgc gcagcgtgcc
    12901 gacgaaatcg gcaacggttt ggtattcggt ctgcctgtcc acaccgatac ggtgtttgac
    12961 ggtaacggga ataccgaccg cgtcctgcat ggcgttgagg cagtcggcaa ccagcccgac
    13021 ttcgttcatc agacacgcgc cgaacgagcc tttctgcacg cgcggactgg ggcagccgca
    13081 gttgaggttg acctcgttgt aaccgtatgc ctcggcggct ttggcggctt tcgccaaatc
    13141 ggacggatcg ctgccgccca gttgcagggc gacgggctgc tcgccttcgt tgaacatcaa
    13201 aaagcggtct ttgtcgccgt aaacaatcgc gccggcgttg accatttcgc tgtacagcca
    13261 agtattgcgg gtaatctggc gcgccatata acggtagtgc ctgtccgtcc agtccaacat
    13321 cggggcgacg gacaggtggc gcgggggaag ggtgtgtgta ttgtcgttca ttgtcgatat
    13381 tttatcattg cggattcaat ttcaatccgg aacaaccttc atttttgggg aaatccgcgt
    13441 catgaaagag tccagcagta tggttaccgg cccgtcgttg cagagcgaca cctgcatatg
    13501 cgtgcggaaa cgccctgttt cgacatgaat tccgtgtccg cgcaacagtt ccgccgttcg
    13561 caggtaaagc tgctgcgcct gttctgcagg tgcggcttgc gaaaacgaag gccgccgccc
    13621 gcttgtcgcg tcggcataaa gcgtaaactg cgacaccagc agcaccgcgc cgccgacatc
    13681 tttcaaagac aggttcagct tgcccgcttc gtcttcaaac acgcgcaaat gggcgatttt
    13741 gtcggcgata tagcgtgcat ctttttctgt gtcgctatgc gttacgccga gtaacacgac
    13801 aaacccgccg tcgattttgc cacaggtttc cgtgccggct tcggacacga catccacctt
    13861 tgcacctacc gttttctgta tgaccgcacg cataagccgt cctccttacc tgaacgtgca
    13921 atgccgtctg aaaggcttca gacggcatcg gtattggaaa tgccattacg gtttgacacc
    13981 gttttcttcc aaaagggcat cgattttcaa gcgggctttt tcctgtctca aaccctgctc
    14041 caactggttt ctgaccaact cgaaaggctg cgcgtcgggg tttttcccga cctcgctgag
    14101 tttgaacaga taatagcgtt cgcccaattt gaccggatcg cgggtaacgt cgccccgatt
    14161 catcgcggca aactgcgaag ccagcggctc gggaagctgc tgcgccataa tgaaaccgtc
    14221 aaaagcctgc tcgtcgttcg gataacgctt catcagccct tcaaaagaca gccctttgag
    14281 caggagctgc tgcgcctgac gcgcctcctc ttcggttgcg aagctgacct gctgcaattt
    14341 gatcatgcgg atttgctgtt cgtaaaactt gtgcagctcg tcttcggaaa ccgtttccga
    14401 acgttccaga aaacggacgt actcctcggc ataaaaagac gcttcggcga ttttaaagcg
    14461 gttttggaca tccttatcct tatccaaacc ttccttcaat gccctgtttt tcaaaacttc
    14521 caaagtttgt agccggcgga cggcatcgtt tcggattgcc tgcccgtccg gtttttggga
    14581 ctgctccgca tgccggtctg cctgctgcat gatctgcgcc accagcgtat ccaccaaagc
    14641 cgggtcgatt tcgggtgctt tggctgccgc aaaacctgcc aacattgcag caataactgc
    14701 ggcagcggtt tttttctgtt tcatggtatt ccctgtcctg cgcggcggca aaacgcctgt
    14761 ctgaacgtct tgccgccata tcccgttttc agaattattt tgcaggtttg atgtttgcct
    14821 tgcccaacag tgcaccgacg gcacggtcaa tccgttccgc ctgaaggttg cccgcaatct
    14881 gtcctttcat ttcatcaaaa gaaggcactt ttacctcgcg gctgtcgttg acataataaa
    14941 cgccgtagaa atcgccgttt ttcagcggcg ttgccgtaaa ttcgcctttt ttcaagtcct
    15001 taattgcctg ataaagcggc ggaacaccct gttccaaatc tttcagcggc acatatccga
    15061 ccggcgcacc ggtctgtttg gtacggtcgt tgagggaata ttgtttcaag acggcatcga
    15121 aacctttttt cgccttcaag tcggcaaccg ctttttttgc attttcttcc ttgtcggtca
    15181 ggatttcgcc caactggact tcctgcgtac ctttgtaaaa accgctgata ttgtcatatg
    15241 cggcttttac ttcctgctcg gaaaccggtt gggttttggc gatatgcaat gcgtatgcct
    15301 cgccgttcaa gccatatttt accgcctgcc aaacggtttt gaaggacggt ttcttgtcgt
    15361 cgcccgactt tttcgcttcg gcacgcaatt tggcaagcgc atttttaaac tctgccgacc
    15421 ggtcgagttt caggcgtttc acttcctgtg cgaccacggt gttgaccact tcgttttcca
    15481 gcagggattg gcgcagttgc ggcgtgtctt cggcacggct gttttccgca cggaatgcgg
    15541 caacctgcgc atcgatgacg gaactgtcga ttttctgacc gttgacggtt gccagcgttt
    15601 gggcaaacag gctgccggaa caggcaagca gtgcaacgga agtcaggatt tttgctttca
    15661 taatcttctc tctttaaatt ttaaattgaa atgttcgggc agacttcatt cagtccgccg
    15721 tatggaaata ctcgtcaggg gtagccgccc ggatgccgag cgcgtgaatc atgtcgcctg
    15781 aaaacaaatc tttgagcagc gatttgaccg ttttctggcg gttcaggcgg cttacgcctt
    15841 caaaacggcc gctgacgacc aaaacggcat aatgtccgcc gccggtattg cccgcgtgtc
    15901 ctttgtgcag atggctctca tcgccgattt ccaacaccag cggatcgagc gtctgcaggc
    15961 gttcgcggat caaatcgacg gccggcatta tttgaacacc gctttaaacg gcttgatcag
    16021 cacttcgctg tacacgcctg catgaacata gggatcgtct tccgcccacg cctctgccgc
    16081 atccaaagac tcgaactgcg ccacaatcaa actgcccgaa acgcggttgg agtcctccgg
    16141 caaaggattc gggcctgccg tcaacagccg gccttccgac ttcagcgtct ccagccgttt
    16201 gaggtgttcg ggacgtgccg ccatacgcgc ttcatacaca ttctccccgt ctgttgccag
    16261 caacataaaa tattccacag tcaatcctcc tttttcagac aggtactcag ataaataccc
    16321 tgaataataa agaaaacaag catcagtgca gtcgagccga acattttata gttgacccat
    16381 tgcgactcga accgggtaaa cacaaaccag ttggcgatac ccataaaaat caggaaaccg
    16441 acccacatat acgtcaattt cgcccatacg gcatccggaa gctgaatctc cctgccgata
    16501 ctcgccttca agccgttttt accggcgagg tggctgcccc acaggaacag cgcgcccagc
    16561 caaaacaaaa cgctcggctt ccacataatg aagcggctgt cgcccaaaac aatggttgcg
    16621 ccaccgaata tcacaatcag caccagtccg acccactgca tcgtatccag ctttttatat
    16681 ttccaataca gaaaagccgc ctgaaccaca ccggcaacca aggcgaccgc cgttgcggca
    16741 atcatgtttt tggtaacggt ataggtggcg aaaaacagga tgacggacaa aaggtcgctg
    16801 acgaatttca tatccaaatg agaaagcaaa tcaaataaac gctaatcata caggcagaac
    16861 cctgtttttg cacctgcaaa accaacaaat tttgttttga ttgatgcaaa tatgcacaga
    16921 tgtttttgaa aaaagatgga gatatgtcat aatttgtaaa aacggcgaat gattgccgtt
    16981 taaaatgtgg cggcggtcgg tacattcaca tattaaatgc ccgccggccg actgccccgc
    17041 cgatgcccga tgatgacgaa atttacagac tgtacgcggt caaaccgtat tcagccgcca
    17101 acccacaggg gatacatctt gaaaaacaac agacaaatca aactgattgc cgcctccgtc
    17161 gcagttgccg catcctttca ggcacatgct ggactgggcg gactgaatat ccagtccaac
    17221 cttgacgaac ccttttccgg cagcattacc gtaaccggcg aagaagccaa agccctgcta
    17281 ggcggcggca gcgttaccgt ttccgaaaaa ggcctgaccg ccaaagtcca caagttgggc
    17341 gacaaagccg tcattgccgt ttcttccgaa caggcagtcc gcgatcccgt cctggtgttc
    17401 cgcatcggcg caggcgcaca ggtacgcgaa tacaccgcca tcctcgatcc tgtcggctac
    17461 tcgcccaaaa ccaaatctgc actttcagac ggcaagacac accgcaaaac cgctccgaca
    17521 gcagagtccc aagaaaatca aaacgccaaa gccctccgca aaaccgataa aaaagacagc
    17581 gcgaacgcag ccgtcaaacc ggcatacaac ggcaaaaccc ataccgtccg caaaggcgaa
    17641 acggtcaaac agattgccgc cgccatccgc ccgaaacacc tgacgctcga acaggttgcc
    17701 gatgcgctgc tgaaggcaaa cccaaatgtt tccgcacacg gcagactgcg tgcgggcagc
    17761 gtgcttcaca ttccgaatct gaacaggatc aaagcggaac aacccaaacc gcaaacggcg
    17821 aaacccaaag ccgaaaccgc atccatgccg tccgaaccgt ccaaacaggc aacggtagag
    17881 aaaccggttg aaaaacctga agcaaaagtt gccgcgcccg aagcaaaagc ggaaaaaccg
    17941 gccgttcgac ccgaacctgt acccgctgca aatactgccg catcggaaac cgctgccgaa
    18001 tccgcccccc aagaagccgc cgcttctgcc atcgacacgc cgaccgacga aaccggtaac
    18061 gccgtttccg aacctgtcga acaggtttct gccgaagaag aaaccgaaag cggactgttt
    18121 gacggtctgt tcggcggttc gtacaccttg ctgcttgccg gcggaggcgc ggcattgatc
    18181 gccctgctgc tgcttttgcg ccttgcccaa tccaaacgcg cgcgccgtac cgaagaatcc
    18241 gtccctgagg aagagcctga ccttgacgac gcggcagacg acggcataga aatcaccttt
    18301 gccgaagtcg aaactccggc aacgcccgaa cccgctccga aaaacgatgt aaacgacaca
    18361 cttgccttag atggggaatc tgaagaagag ttatcggcaa aacaaacgtt cgatgtcgaa
    18421 accgatacgc cttccaaccg catcgacttg gatttcgaca gcctggcagc cgcgcaaaac
    18481 ggcattttat ccggcgcact tacgcaggat gaagaaaccc aaaaacgcgc ggatgccgat
    18541 tggaacgcca tcgaatccac agacagcgtg tacgagcccg agaccttcaa cccgtacaac
    18601 cctgtcgaaa tcgtcatcga cacgcccgaa ccggaatctg tcgcccaaac tgccgaaaac
    18661 aaaccggaaa ccgtcgatac cgatttctcc gacaacctgc cctcaaacaa ccatatcggc
    18721 acagaagaaa cagcttccgc aaaacctgcc tcaccctccg gactggcagg cttcctgaag
    18781 gcttcctcgc ccgaaaccat cttggaaaaa acagttgccg aagtccaaac accggaagag
    18841 ttgcacgatt tcctgaaagt gtacgaaacc gatgccgtcg cggaaactgc gcctgaaacg
    18901 cccgatttca acgccgccgc agacgatttg tccgcattgc ttcaacctgc cgaagcaccg
    18961 tccgttgagg aaaatgtaac ggaaaccgtt gccgaaacac ccgatttcaa cgccaccgca
    19021 gacgatttgt ccgcattact tcaaccttct gaagtacctg ccgttgagga aaatgcagcg
    19081 gaaatcgttg ccgatgattt gtccgcactg ttgcaacctg ctgaagcacc ggccgttgag
    19141 gaaaatgtaa cgaaaaccgt tgccgaaata cctgatttca acgccaccgc agacgatttg
    19201 tccgcattac ttcaaccttc tgaagtacct gccgttgagg aaaatgcagc ggaaaccgtt
    19261 gccgatgatt tgtccgcact gttgcaacct gctgaagcac cggccgttga ggaaaatgca
    19321 gcggaaatca ctttggaaac gcctgattcc aacacctctg aagcagacgc tttgcccgac
    19381 ttcctgaaag acggcgagga ggaaacggta gattggagca tctacctctc ggaagaaaat
    19441 atcccaaata atgcagatac cagtttccct tcggaatctg taggttctga cgcgccttcc
    19501 gaagcgaaat acgaccttgc cgaaatgtat ctcgaaatcg gcgaccgcga tgccgctgcc
    19561 gagacagtgc agaaattgct ggaagaagcg gaaggcgacg tactcaaacg tgcccaagca
    19621 ttggcgcagg aattgggtat ttgattccca actgcccttt cgcagatcaa ggatgccgtt
    19681 tcagacggca tctttttttg ccttatcggt gtaacggata aagtttgaac cggcacaggc
    19741 tcaaacagca ggccgacggc aacaaaatgc cgtctgaaac ccctaaaggc ttcagacggc
    19801 attggcggcg gcggtatcag gcagtttgat aggcaaccga atcgtcaccg cttttctttt
    19861 gaatgaactc gattttgtat ccgtcggggt cttcgacgaa ggctatcacg gttgtgccgt
    19921 gtttcatcgg gccggcttcg cggacgacgt ttccgccctg ccgcttcaca cgttcgcagg
    19981 cttcgtaggc atcgtccact tcaaccgcga tgtgtccgta ggcgttgccc aagtcgtatc
    20041 gttccgtatc ccagttgtgc gtcagttcca aaaccgtgct gtcggtttcg tcgccgtaac
    20101 cgacgaaggc aagggtaaat ctgccttcgg gataatcttt tcggcggagc agtttcatac
    20161 ccaaaacgtt ttggtagaaa tcgagggatt tttcgagatt gcccacgcgg agcatagtat
    20221 ggagtaagcg catttttgtg ttcctctcgg tagtggttaa acttcgattt tgtttggcgt
    20281 aaacgtctgc catttgttgc aggcaggaca atgccagaaa aagacttgtg atttgaagtg
    20341 gcagtttcgg caccggtaca tcacgctgcg ctgtagctgc cgtccgataa ccgaacgcat
    20401 catatcggca tcggctttcc aagccggatc caaatcgctg agtttcaaac caagcaggcg
    20461 gtacacgccg ttgaggtcgg gcttgcggcg gacaagctcg acggcggttt gcgcggcttc
    20521 tttctcgcac ttaagcagca gggatttctc gtacacgaca ttgatcaggt caagttcggg
    20581 aaacgtctgc atatatcctg tcagacggtt caagccttct tcaggttttc cctgcgcggc
    20641 ataggcttcg taaagcttct cgccgaccat gctcaagtat gcatggtttt gctgctcgat
    20701 ggcggcatag gcttcgacgg cggcagggaa attgccttgt cggtgttcga tgtcgcccaa
    20761 aatcatgttg gcacgggtgc attttttgtt ggcttcgagt gccttgccga cattgaaacg
    20821 cgcgacatcg aaattggact tgaacagcgc ggcttgggca agttcgcaat aaaactgggc
    20881 gatttcaaac tgataggtct gatcgtcatg gctgagcagc cgggcggttt caaccgcttt
    20941 ttcccaatcc ctgtcctgtt ggtagatatt gagcaggtgc tgtctggctt cacgcgccat
    21001 tttaccgtct tgcagcccca aaaaaatctg ttcggcacga tcgaccaacc ccgcactttg
    21061 gtagttttgc gccaattcaa acaggacgcg cgcgcgcttt tcgccgaccg tatcgggaga
    21121 atcgagcatt gtccggtgta tgttgatggc tttgtcgttt tcgccacgct gacggtaaag
    21181 cttgcctagg gtgaggttta ggtcgtatga ctgaggctgc tgatcaatga cctctgccaa
    21241 ttccctggcg gcacgtccgc tgttgcggtc aaccaaggca tccagacttt tataaaatcc
    21301 cgacggtatg ctttttgcct gctttaatac agtcttcata tccacacggg cggcaaacca
    21361 gcccatcgcg aagaaaacgg gcaaaaggat aatcggcagc aggataatcc acaattcatt
    21421 gtccatatcg gctttctcaa ggctgtttgg cagattcggg cgcattttgc gccggtggtg
    21481 cggtcagctc tttccccgtc aaacgcgcat ttttctttac ttcggcacgc aacctgccgt
    21541 tctcgccacg taacgacaac aaccgtccga acaaggcaaa cattccaaaa acaataccga
    21601 ctacaaatgc gccgaacaat acgacaatca gcggcaaatc gaatttttgc cccggcaggt
    21661 aggaaaaggt aacggcatcc gtattaatga cggcaagcag caggaagagc agcaggataa
    21721 tgattttgat gacggtatag ataagtttca tgtttgctcc gtaaataaaa acgccgcccg
    21781 gacagcttcg cactctgaac caagattgcg ctagtttaaa cgatttgcac ggtttcggat
    21841 aggatgcggc agcgtgttcg gacgacatac ggagtatgcg attgccgaca attttaccaa
    21901 atacaccgtt cctttccatt tgaaaaataa cggattggac accgcatcga cagaaaaacc
    21961 cgccgcgcac ttgtcaaaac cctgtttgca ggcgtatctt tacaatcttc aaattcaaac
    22021 cgttcattga aacatatcag aataagaaag gctttacatc atgagcagac ccgtacccgc
    22081 cgtattcggc agcgtttttc acagtcaaat gcccgtcctc gcctaccgcg aaggcaaatg
    22141 gcagccgacc gaatggcaat cttcccaaga cctctccctc gtaccgggtg cgcacgccct
    22201 gcactacggc agcgaatgtt tcgagggact gaaagccttc cgtcaggcag acggtaaaat
    22261 cgtactgttc cgtccgactg ccaatatcgc gcgtatgcgg caaagtgcgg acattttgca
    22321 cctgccccgc cccgaaaccc aagcctatct cgatgctttg gtcgaactgg tcaaacgtgc
    22381 cgccgacgaa attcccgatg cgcctgccgc cctgtacctg cgcccgacct taatcggtac
    22441 cgatcccgtc atcggcaagg ccggttctcc ttccgaaacc gccctgctgt atattttggc
    22501 ttcccccgtc ggcgactatt tcaaagtcgg ctcgcccgtc aaaattttag tggaaaccga
    22561 acacatccgc tgcgccccgc atatgggccg cgtcaaatgc ggcggcaact acgcttccgc
    22621 catgcactgg gtgctgaagg cgaaagccga atacggcgca aaccaagtcc tcttctgccc
    22681 gaacggcgac gtgcaggaaa ccggcgcgtc caactttatc ctgattaacg gcgatgaaat
    22741 cattaccaaa ccgctgaccg acgaattttt gcacggcgta acccgcgatt ccgtactgac
    22801 ggttgccaaa gatttgggct ataccgtcag cgaacgcaat ttcacggttg acgaactcaa
    22861 agctgcggtg gaaaacggtg cggaggcgat tctgaccggt acggcagccg tcatctcgcc
    22921 cgttacttcc ttcgtcatcg gcggcaaaga aatcgaagtg aaaagccaag aacgcggcta
    22981 tgccatccgt aaggcgatta ccgacatcca gtatggtttg gcggaagaca aatacggctg
    23041 gctggttgaa gtgtgctgat gctttaaata aaaaaatgcc gtctgaaacc cgtttggcgt
    23101 ttcagacggc attttcgcgt tcggaccatt tccgctgcac ctgcagcaag tcgggcattt
    23161 ccctagaaat acgcttcaga cagcaaaccg ccgcccgaaa ccgatacgcg gtacggacgg
    23221 cgcacggatt tgaaaacggc ggattatccc tcggtgctca aggcattaat gctgtaaccg
    23281 ccgtcaacgt aagtgatttc gccggtaatg ccggacgaca ggtcggacag caggaaggcg
    23341 gcggtattgc cgacttcttc aatggtaacg ttgcggcgca gcggattgtg ggcggcgacg
    23401 tgtcccaaaa gtttgccgaa atcggcgata ccggaggcgg caagcgtttt aatcgggccg
    23461 gcggaaatgc cgttgcagcg gatgccttct ttgcccagac aggcggcggt aaaacggatg
    23521 cccgcctcaa ggctggcttt tgccataccc atcacgttgt aatttggaat cgcgcgcacc
    23581 gcgcccaagt agctcagggc aacgatggcg gagtttctgc cgcgcatcat cggacgggcg
    23641 gcttttgcca atgcgggcag gctgtatgcg gaaatttcgt gtgcggtgtt gaacgcttcg
    23701 cggctgatgc tgtcgaggaa gtcgccgctc aaggcttctt tcggcgcgaa gccgatggaa
    23761 tgcaccaaac cgtccaagcc gtcccaatgt ttgcccaagt cggcgaacac ttggttgatt
    23821 tcgtcgtcgc tggcgacatc gcagcggaat acgagttcgg aacccaattc cgccgccatt
    23881 ttgcggacgc gctcttccag tttgtccaca acgtaggtaa acgccagttc cgcgccttgt
    23941 tcgcggcagg ctttggcgat gccataagcg atggaacgct cggaaatcat gccggtaatc
    24001 agaatttttt tgccttgcag aaaacccatt ttgtatcctt caaatagtgt tcggttcatt
    24061 ctaaaccgtt gattatagca aattgtccct gtttctgtgt tttcacgttg cagcatgcaa
    24121 acggcaatgc cgtccgaagc ggatttcaga cggcatcgga cgtttcaaat acggtttaag
    24181 gcatcagatg ccgcgcaaca attcgttcac gctggttttc gcacgggttt gcgcgtccac
    24241 gcgtttgacg atgacggcgc agtaaaggct gtggctgccg tctttggaag gcatactgcc
    24301 ggacacgacg accgaaccgg ccggtacgcg accttggtag atttcgccgg tggtgcggtc
    24361 gaagattttg gtggattgac cgatgaacac gcccatcgaa atcacgctgc cttcttcgac
    24421 aatcacgcct tcgacgattt cggaacgcgc accgatgaag cagttgtctt caatgatggt
    24481 gggtgcagcc tgcaggggtt cgagtacgcc gccgatgccg acaccgccgc tcaagtggac
    24541 gtttttgccg atttgcgcgc aagagccgac ggtcgcccaa gtatcaacca tcgcgccttc
    24601 gtcgacgtat gcgccgatgt tgacgtaaga cggcatcagc acgacatttt tcgccacaaa
    24661 gctgccgcgt cgggcaaccg cacccggaac ggcgcggaag cctgcgtttt tgaactcgtc
    24721 ttcagaccag tcggcaaact tggtcggcac tttgtcgaag tatttgttca cgccgtcgtt
    24781 gaggacttcg ttgtcttgga tgcggaagga cagcaacacg gcttttttcg cccattcgtt
    24841 gactttccac tcgcccacgc ccaagcgttc ggcaacgcgc agtttgcctg aatcgagttg
    24901 gcggatggtt tccaacacgg cttctttaac ttcgggagca acagtggacg gggtgatgtc
    24961 cgcgcggttt tcaaaggcgg tttcgataat gttttgcaaa gacataatgt ttccttatgt
    25021 gagatgtttc gtgggacgct tgccggtatt ttcaagcagt atgttcattt gaaatgaatg
    25081 cgctattgtg ccacgcaaac gcatattcgt cagcaatacg cgggcggttg ccgaacattg
    25141 gcgtttcgga atcgggcggg cggcaggtat tgccttttcc gcgcccgaat caatacagaa
    25201 aggcggcagt acttttatgc cgcgccgccg cctttcagac ggcattcgcg gtaaaacgcc
    25261 atcagccctt cggtcgaggc atcgtgtacg gacgtgccgc cttccagttc gccgatgatg
    25321 gttttcgcca actgtttgcc gtattccaca ccccactgat cgaaggggtt gacgttccat
    25381 atcgtgcctt ggacgaaggt tttgtgttcg taagccgcca tcagcatacc cagattgtag
    25441 ggcgtgaggc ggtcgagcag gatgctgtta ctggggcggt tgccggggaa ctctttgtgc
    25501 ggcgcgaggc gttcgcgttc cgcttcgggc aaatctgcca attcggcgcg tgcttcgtcc
    25561 aaggttttgc ctttcatcag ggcttcagcc tgtgcaaagg cgttggcaac ggtaaaacgg
    25621 ctgcgtcctt cctccacgcc ctgcgccgtc atcgggacga taaagtcgca gggaatcagg
    25681 cgcgtgcctt gatgaagcag ttggaaatag gcgtgctggc agttgacccc ttcaccgccg
    25741 aacacgatgc cgcccgtttt gcacacggcg ggactgccgt ctgaagcgcg gcttttgccc
    25801 aaactctcca tatcgagctg gttcagccac gccggcagca ggcgcaggtt gtggctgtac
    25861 ggaacggcgg tctgcccgtc cgcgtgttgg aaattgttat accacacggc aatcagtgcc
    25921 atcagaacgg gaatgttgcg gcgcggcggc gtgtggaaaa aatggctgtc catcgcgtgc
    25981 gcccccgcca acaactcgcg gaaacgcgcc ccgccgaccg caaccatcac gggcaaaccg
    26041 acgggcgacc agacggaata gcgtccgccc acccagtcgt acatcgcaaa cacgcgttcc
    26101 gccgcgatac cgaaagccgc agctgccgca gtgtcggcag acaccgcgca aaaatggcac
    26161 gccgtttcgg attccgagaa ccccgcaccg cgataccacg ccttgactgc ctgtgcattg
    26221 agcagggttt ccggtgtttt gaaggacttg ctggcaacgc aaaacactgt cgtttcgggg
    26281 ttcagacggc ataaaaccgc atccaggcag gcaggatcgg cgttggcggc aaaatggacg
    26341 gcgatatgcc gtctgaacgg ctcaagtgcc tgcacgcaca ttgccggccc gaggtcggat
    26401 ccgcctatgc cgatgtggac aaaatccgta atccgttttc cggttgtccc ctgatacgaa
    26461 ccgtcgtcca aactgtgtgc aaacttcaac gcacgattta actcgcggcg gatttcgggc
    26521 aacacgtccc tgccgtccgc ataaacggca cccgcaccgt cgggcaggcg caaagccgta
    26581 tgcagcgcgg cacgcccctc actgttgttg accttcgcac ccgtccgcaa agcacgcatt
    26641 ttcccttcca aatccgccgc atcggcaaga ttgcagagca gttgcagcgt atcttcgccc
    26701 aaacggtttt tgctgtaatc gaacaacatc ccgtccaaac gctcgtgcat acgctcaaaa
    26761 cggtcgggtt cgcaggcaaa gcggtcgcgc aaaaggacat gacgcgtatc ctgataatgg
    26821 cgttcgagcg catgccatgc acgggtaaaa gcattcatct gttttccttg atttttcaga
    26881 accggattaa aatgtagcag aatgtagttt aacaaacggc agcggctttg gcgaatctcc
    26941 ggaacaccgc ccgccgcgtc caatagcacc ttatttccat atggcgaaaa tatagtgaat
    27001 taacaaaaat caggacaagg cggcgagccg cagacagtac aaatagtatg gaaccgattc
    27061 acttggtgct tcagcacctt agagaatcgt tctctcttga gctaaggcga gccaacgccg
    27121 taccggtttt tgttaattca ctataccctc cggccggcat ttgacccgca acaatatcct
    27181 gcaagattta ttgtgtacgc ataaatgccg gacagccgcc taaataaggt ataatcgcat
    27241 ccgattctgt cccgtttgcc gcccgcaggc gggatgtcgg cggttttcat cttaccggga
    27301 acacgccggt actgtttttt aacgatgttt cttacatttt attccaatta ctttacgggg
    27361 ctagaatatg gctaaaaacg gaggattttc tttgttcgca aagaaagaaa aacgctttat
    27421 ctttgaaggc aggcattccg cctccgacaa actggtcaac ggcgaagtat ccgcgtttac
    27481 cgaagaagag gcgcgcaaaa aactggcaaa acgcggcatc cgcccgttgc agattacccg
    27541 tgtgaaaaca agctccaagc gcaaaatcac acaagaagac atcaccgttt tcacccgcca
    27601 gctgtccacg atgattaaag cgggcctgcc gctgatgcag gcatttgaaa tcgtggcgcg
    27661 cggacacggc aacccgtcta tgacggaaat gctgatggaa atccgaggcg aagtggaaca
    27721 gggcagctcg ttgagccgcg cattctcaaa ccacccgaaa tatttcgacc gcttctactg
    27781 caacctggtt gcggcgggcg aaacgggcgg cgtattggaa agcctgctgg acaaattggc
    27841 aatttacaaa gaaaaaaccc aggccatccg caaaaaggta aaaaccgcgc tgacctatcc
    27901 ggtatccgtc atcgccgtcg ccatcggttt ggtattcgtg atgatgattt tcgtactgcc
    27961 cgcctttaaa gaagtttacg ccaatatggg cgcggagctt cccgcactga cccaaacagt
    28021 gatggatatg tccgactttt tcgtctcata cggctggatg gtgctgatcg cactgggctt
    28081 tgccatatac ggcttcctta aattgaaggc gcgttcgatt aaaatccaac ggcgtatgga
    28141 tgccatactg ctgcgtatgc cgattttcgg agacattgtc cgcaaaggga cgattgcccg
    28201 ctggggcagg acgacggcga cgctgattgc ggcaggcgtg cctttggtcg atgtattgga
    28261 ctccactgcc ggcgcggcgg gcaatttaat ctatgaagaa gccacccggg aaatccgtac
    28321 gcgcgtcatc caaggtttgt ctatgacttc ggggatgcgt gcgacggaac tgttccccaa
    28381 tatgatgttg cagatgtctt ccatcggcga ggaatcgggt tctttggacg atatgctcaa
    28441 caaagccgcc gaattttacg aagacgaggt ggacaatgcg gtcggcaggc tgtccgctat
    28501 gatggagccg atcattatcg tgattttagg cttggtcatc ggcacgcttc tggtcgccat
    28561 gtatctgccg ctgtttaatt tgggcaacgt ggtcgcctga tttgccgcac agatccggcg
    28621 cggattcgct ctgcgccggt ttgtttttgt tttgaataca ttgagaacaa aatatgtctg
    28681 atttgtctgt attgtcgccg tttgccgtgc ctttggcagc ggtgttcggg ctgctggtcg
    28741 gaagtttctt aaatgtcgtc atttaccgcg tgccggtcat gatggaacgc ggctggacgg
    28801 tatttgccaa agaatattta aacctgccgc tgaccgaaga ggaaagccgt accttcaacc
    28861 tgatgaagcc ggattcctgc tgtcccaaat gtcgcgtgcc gatacgcgcg tggcagaaca
    28921 tcccgattgt cagctacctg ctcctgcgcg gcaaatgcgc ttcctgccaa accaaaatca
    28981 gcatacgtta tcccttaatc gagctgctga ccggcgtatt gttcgggctg atcgcctggc
    29041 aatacggctg gtcttggatt acgctgggcg gattggtact gaccgcgttt ctgatttccc
    29101 tgacctttat cgatgcggac acccaatacc tgcccgactc gatgacactg ctcttgattt
    29161 ggctgggact gatatttaat ttggacggcg gcttcgtgcc tttgcagtct gccgttttag
    29221 gtgcggttgc cggctatggt tcgatatggc ttttatgcgc ggtatataaa ctgattaaag
    29281 gcgaaaccgg tatgggcaac ggagatttca aactgattgc cgcattgggc gcgtggctcg
    29341 gcatatccgc attgcccgtg ctgatttttg tttcctcgct gatcggtttg gtcgcggcaa
    29401 tcgttatgcg cgtcgccaag gggcagcatt ttgccttcgg ccccgcactg acagtttcgg
    29461 gctggataat ttttacggca aacgattccg tatggcgggc ggtcaactgg tggctgaccc
    29521 atccggtgct gtaagatgac ggtatgggtc ggactgaccg gcggaatcgg cagcggcaaa
    29581 tcggcagccg cgcaatgttt tgccgatttg ggcgtgccgc gcatcgatgc ggacgcggcg
    29641 gcgcactcgc tgacggcttc agacggcatc gccctgccgg aaatcaggcg gctgttcggc
    29701 gacaccgttt tcgacacaca gggtttgttg cggcgcgaca tattgcgtaa agaagtcttt
    29761 gcctccccat cgcaaaaagc cttgctcgaa tccgtgatgt tgccgctgat tttctcagaa
    29821 atcaaaaagc agcaagaaac ctttaccgat gcggcttacg gcattgtcga aattccgctg
    29881 ctgacggaaa agcgtcaatt tatcagcctg atacggcgtg tcctgaccat aagcgcacct
    29941 ttggaaaaac gtatcggcag ggtgatggcc cgcagcgggc tgacgcgcgg cgaagtggcg
    30001 gacatcatca gccatcaggc atccgaatcc gaacgcctgc tgcttgcaga cgatgtgctg
    30061 ctcaatgacg gcagcctcaa aagcctgcgt gagaaaacaa tgcgcctgca cgcgttttat
    30121 tcagggattt tcgcctcaaa accaacacaa ggaaaacaca atgactgaat cgcggcaaac
    30181 acgccttcaa gtcaaatgtc cgacctgtca aacagcagta gtatgggaac ccgaaaacgc
    30241 attccgcccc ttctgttcgc aacgctgcaa actgatcgac ttgggcggat gggcagacgg
    30301 gaaatatacg gtttccggcc aaacggaaag tttgccggaa atatccgaac ccgacatggc
    30361 ataccgctga ccgccccgcc ttcccggcaa acaccctgaa agtcaaatgc cgtctgaaac
    30421 aaacacgctt cagacggcat tttcattctc aaacctaatc gttggtattt gccgttacct
    30481 cttccaatga agtaatgccc tgcataactt tcaaaatacc ggcccggcgc aaatccacca
    30541 taccctcctt ataggcaacg tccaaaatat ccacttccgt accgttgttc ataatcacac
    30601 gctgcatttc ttcgctgatg ggcataacct catacacgcc cgcacgcccc ttataaccct
    30661 gcccccggca acggtcgcaa ccgacggcgc ggtaaagttt ccaatctttt gcaaggtcct
    30721 catcggtgaa gccgacttcc ttcaaagcag aggcagacgg gcgttccact tcctgtttgc
    30781 agctcgaaca cagcctgcgt aaaagacgct gcgccataat caggctgacc gaactggcaa
    30841 tattaaacgg cgcgacaccc atattcagca tacgcgacaa cgtcgccggc gcattattgg
    30901 tgtgcagggt ggaaaacacc atatgccctg tttgtgccgc cttaatcgca atatcggcag
    30961 tttccaaatc acgaatctca ccgaccataa tgatgtccgg gtcctgacgc aggaaagact
    31021 tcaaagcagc ggcaaaagtc aggccctgct tatcattgac gttaacctga ttgatgcccg
    31081 gcaggttaat ctcggcaggg tcttccgccg ttgcaatatt taccgactcc gtattcaaaa
    31141 tattcaaaca ggtatagagc gacaccgtct tacccgaacc cgtcggaccg gttaccagca
    31201 ccatcccgta gggacggtga atcgcttcca acaacaattt tttctgaaac ggctcaaaac
    31261 cgagctggtc gatgttcaaa gacgcggcat cggaattcaa aatccgcatc acgacctttt
    31321 cgccaaacag cgtcggcaat gtgctgacac ggaaatcgac aggcttgccg cccttttgaa
    31381 aggtcagctg catcctgccg tcctgcggta tccgtttttc ggaaatgtcc aaacgcgaca
    31441 ttaccttaat ccgggaagca agctgccccc ttaccgcaat gggcggctga accacctcgc
    31501 ggagctgccc gtccacacgg aaacggatac gggcattgtg ttcgtaaaac tcgaaatgga
    31561 tgtccgatgc cccgctgcgc aaggcatccg acaaagtctt atggataaac ctcggaacag
    31621 ggccgtcttc tgcctcctcg ttgtcgatat acagggtgtg gctttcctct tcctcctgcc
    31681 cctccccaag ctcctgaagc agcgatgtcg aacgcgaacc cacccaatcg agcaaacccg
    31741 ccaactggtc atcctcgaca atgaccaact caacctcaat ccctgcggca gaaacggttt
    31801 tctgaatttg cggcatctgt gtcggatcgg aaaccgcaaa aaatactttg tcgccccgac
    31861 ggaaaaccgg cacacagtgg aactccacca tctgctcctc cgtcaacacc cccatcagca
    31921 ccctgtggcg cggataatga cgcaaatcaa gaatcgaata actgaacacc ctcgcaatca
    31981 atgccgcaag cgacttgggc gaaatgacac cgtctgaaaa cagcatcggc aacacttcct
    32041 tacccgcctg cgactcattg tagtaatgct cggcctgctc aacagtaacc acctggtttt
    32101 gaaccagaat cctcagcaaa cctacgctca tacgacctta tccccaaatt tattcattgt
    32161 tatacctgta cagctt
//
LOCUS       DAWFAN010000005        25228 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWFAN010000005 DAWFAN010000000
VERSION     DAWFAN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.005"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            1..539
                     /locus_tag="WGO65_000171"
                     /pseudo
     CDS             1..539
                     /locus_tag="WGO65_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            596..1669
                     /locus_tag="WGO65_000172"
     CDS             596..1669
                     /locus_tag="WGO65_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0326537.1"
                     /translation="MRPFFVGAAVLAILGALVFFINPGAIVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCAR
                     LIWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNVVYKNIAITFLLLHAAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGIMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRAVLMNVNPIFFITVPAILTAA
                     VFVLYLLTFVPIFRANAFTDDPE"
     gene            complement(1716..3905)
                     /locus_tag="WGO65_000173"
     CDS             complement(1716..3905)
                     /locus_tag="WGO65_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ0326538.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILGVQTTFSDEPLLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYVVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            4044..4826
                     /locus_tag="WGO65_000174"
     CDS             4044..4826
                     /locus_tag="WGO65_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ0326539.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(5144..7078)
                     /locus_tag="WGO65_000175"
     CDS             complement(5144..7078)
                     /locus_tag="WGO65_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ0326540.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGMGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASKVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGKIRTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(7144..8322)
                     /locus_tag="WGO65_000176"
     CDS             complement(7144..8322)
                     /locus_tag="WGO65_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ0326541.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNSDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8548..8937
                     /locus_tag="WGO65_000177"
     CDS             8548..8937
                     /locus_tag="WGO65_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0326542.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFDNYNQAKLKQNKYFNITLSEVSPDHFTLQAAPDPMTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            9221..10267
                     /gene="adhP"
                     /locus_tag="WGO65_000178"
     CDS             9221..10267
                     /gene="adhP"
                     /locus_tag="WGO65_000178"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ0326543.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATYCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(10432..13917)
                     /gene="smc"
                     /locus_tag="WGO65_000179"
     CDS             complement(10432..13917)
                     /gene="smc"
                     /locus_tag="WGO65_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ0326544.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHTGQTIAGHEEHKGRLKQENQALNLPDEAETAAAQEAAALLQNR
                     QEHYEEQIIAAEEALHAAREAFQTASSRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDDLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSADEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQSLDEEYIVVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            14166..14888
                     /gene="lpxH"
                     /locus_tag="WGO65_000180"
     CDS             14166..14888
                     /gene="lpxH"
                     /locus_tag="WGO65_000180"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ0326545.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSIAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDKAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            15157..16743
                     /locus_tag="WGO65_000181"
     CDS             15157..16743
                     /locus_tag="WGO65_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0326546.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16911..17114)
                     /locus_tag="WGO65_000182"
     CDS             complement(16911..17114)
                     /locus_tag="WGO65_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ0326547.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            17230..17727
                     /locus_tag="WGO65_000183"
     CDS             17230..17727
                     /locus_tag="WGO65_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ0326548.1"
                     /translation="MTALTLPEDIRQQEPSALLYPLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            17809..19002
                     /locus_tag="WGO65_000184"
     CDS             17809..19002
                     /locus_tag="WGO65_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0326549.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQNFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(19406..20341)
                     /gene="hemC"
                     /locus_tag="WGO65_000185"
     CDS             complement(19406..20341)
                     /gene="hemC"
                     /locus_tag="WGO65_000185"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ0326550.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVILQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            20460..20975
                     /gene="ybeY"
                     /locus_tag="WGO65_000186"
     CDS             20460..20975
                     /gene="ybeY"
                     /locus_tag="WGO65_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ0326551.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KSGYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            20977..21801
                     /locus_tag="WGO65_000187"
     CDS             20977..21801
                     /locus_tag="WGO65_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ0326552.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVIIGSLQFTVARADNRRLHTLMATRVK"
     gene            complement(21944..23323)
                     /gene="nhaC"
                     /locus_tag="WGO65_000188"
     CDS             complement(21944..23323)
                     /gene="nhaC"
                     /locus_tag="WGO65_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ0326553.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHCYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23614..24837)
                     /locus_tag="WGO65_000189"
     CDS             complement(23614..24837)
                     /locus_tag="WGO65_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ0326554.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         5777 a         7199 c         6533 g         5719 t
ORIGIN      
        1 cggcggatat gcggactgcc gagaaaaacc gtctgcatta ggttcatgaa acgcaagtcg
       61 gaagcgtcaa acaactgatt gcccattttg accggctgat tgacgaattg gacaaacaaa
      121 tcgacgacca cacccacacg cattttgacg gcaaagccca agtggcggag caaatcaaag
      181 gcatcggttc gataacgacg gctacgctga tggcgatgct gcccgaattg gggcggctgt
      241 cgcacaaacg gatagcgagt ttggccggca ttgccccgca cccgagggag agcggggaaa
      301 ccaaattcaa aagccgctgc tttggcggaa agtctgcggt acgtaaggcg ctgtatatgg
      361 ctaccgtggc agcgacacgt tttgaaccgc ttattcggga tttctaccaa cgcctgccgt
      421 ccgagggtaa gccgtataag gttgccgtta cggcatgtat gcgcaaactg ctgacgatat
      481 cgaatgcccg gatgcgtgat tattttgccg aaaacgatac cgccgaaaac ggtatctaaa
      541 cggcttgatt tgagttttgg tatttttgcc cgacggggtg aaaaatacag ttgctatgcg
      601 gccgtttttc gtcggcgcgg cggtgcttgc catactcggt gcgctggtgt ttttcatcaa
      661 ccccggtgcc atcgtcctgc accgccaaat tttcttggaa cttatgctgc cggcggcata
      721 cggcggtttt ttgactgcgg ctttgttgga ctggacgggt ttttcgggta acctgaaacc
      781 tgtcgcgact ttgatggcgg cattattgct cgccgcatcc gctatactgc ccttttcgcc
      841 gcaaactgcc tcgtttttcg tcgccgccta ttggctggtg ttgctgctgt tctgcgcccg
      901 gctgatttgg ctagaccgaa acaccgacaa cttcgccctg ctaatgttac ttgccgcgtt
      961 cactgttttt cagacggcat atgccgtcag cggcgatttg aacctgttgc gcgcgcaagt
     1021 gcatctaaat atggcggcgg tgatgttcgt atccgtgcgc gtcagtattc ttttgggcgc
     1081 ggaagccctg aaagaatgcc gtctgaaaga cccagtattc atccccaatg tcgtctataa
     1141 aaacatcgcc attactttcc tgctcttgca cgccgccgcc gaactttggc tgcccgcgca
     1201 aaccgccggt tttaccgcgc tcgccgtcgg cttcatcctg ctcgccaagc tgcgtgagct
     1261 tcaccatcac gaactcttac gtaaacacta cgtccgcact tattacctgc tccaactctt
     1321 tgccgccgca ggctatttgt ggacaggcgc ggcgaaatta caaaacctgc ccgcctccgc
     1381 gcccctgcac ctgattaccc tcggcggcat aatgggcggc gtgatgatgg tatggctgac
     1441 tgccggactg tggcacagcg gttttaccaa actcgactac cccaaactct gccgcatcgc
     1501 cgtccccatc ctcttcgccg ccgccgtctc gcgcgctgtt ttaatgaacg tgaacccgat
     1561 attcttcatc accgtccccg cgattctgac cgccgccgtg ttcgtgcttt acctgctgac
     1621 attcgtaccg atctttcggg cgaacgcgtt tacagacgat ccggaataaa aatgccgtct
     1681 gaaacggttt tcagacggca tcgtattgcc gataatcaag cctcctgcgg atcgacatcc
     1741 accgaccatc taattttgcc gtcgcggttt tgctgcaaca cctgcaccca caaactcacg
     1801 gcacggtgca aatcctgtcg ggacggggat tcgaggaaaa tttgcgcgcg ttcgcgttcg
     1861 gcgaggcgca ccatcagcat gggggcggcg ccaaactggg aaacgcttgc gggcaacagc
     1921 ggggcgaggg tttctttggc ggcattgaga aattccagcg catcggcaac acgcggcgcg
     1981 tccgcgcgga cggcggtctg aaaaccgaag ggcggcatgg cgaacatttg ccgttcgttc
     2041 aattcgtttt cggcaaacac gacgtagtcc tgcgctttga cggcggcgaa gacgggatgt
     2101 tcgggcagtt gggtctgtat cagcaccttg ccgggcttgt cggcgcgccc cgccctgccg
     2161 gacacctgca tcagctcggc aaacagcctt tccggcgcgc ggaaatccgc gctgtacagg
     2221 ctgccgtcgg cgttcaatac gataacgagg ttgagccgcg cgaaatcatg acctttggcg
     2281 agcatctgcg tgccgaccag aatatcgatt tcgttgtcgg cgatgcggcg gtacaaatcc
     2341 gcccagtcgt ttttgtgcgc cgtactgtcc ctatcgacgc ggacgacggc tgccttgggc
     2401 aggaaggcgc gcagggtttc ttcgacgcgc tgcgtgccgt ggccgacggc ggtcaggtct
     2461 tggttgccgc agtcggggca tttgtacggg atgggttcgc ggtggtcgca gtggtggcag
     2521 cgcagttggc gggcgcgttg gtgcagcacc attttggcgg agcagttcgg gcagccgaag
     2581 gtatgaccgc agtcgccgca aaacagcgcg ggcgcgaagc cgcgccggtt aaggtacacc
     2641 agcgacatac cgcctgcttc aaagttctgt ttcaaaagct gcaaggcttg cggcgagaag
     2701 ccgttgtcga gtttcagacg gcctacgttg agaatgtcca cttgcggcag ttgcgcggcg
     2761 gtatgggcgc gttcggtcag ttgcagcagg cggtacgcgc cactttgcgc cttgtgccag
     2821 ctctccaagc tgggcgtggc actgcccaac acgacggggc agccgccctg cttcgcccgc
     2881 cacaccgcca aatcgcgggc atgatagcgc aattcgttgt cttgtttgaa cgagccgtcg
     2941 tgttcctcat cgaccacaat cagcccgaca tcatccatcg gcgtgaacac tgccagccgc
     3001 gtgccgatca ccagcttcgc ctgccctaac atcgcgcgca gataatcctg cgtgcgcttg
     3061 cctgccgcca tctgactgtg caacacggcg gtcggcacgt cggcaaaacg gttttccacc
     3121 cgcttcaaaa gctgcggcgt gaggttgatt tcgggcaaca gaaacaacac ctgccgcccc
     3181 tgagccaaca ctttcgccat cgcatcaaaa tacacctcgg tcttgccgct gccggtgatg
     3241 ccgtacagca gaaacggctg gaagcgtccc aatgccgtct gaatggcatc ggaagccttc
     3301 tgctggtcag catttaatac aaattcagag tgcgaagcct gcccattaca cgaccttaaa
     3361 acgggcttcg ccgcttccgt cgtttcaatc caaccctgct ccgcccaatc ttcgattaat
     3421 ttcgccgcct gcgcgtttac ctgcttcaac gctgccatcg tcatttcgcc cgacagcagc
     3481 gcgtcccaca aagccgcttt tttgttgaac cgcgccggcg gcggcgtttg cgccctgccc
     3541 gcttcgttca acgcgtaaaa caagggcggc tgcggcattt ccaccgcgcg cgtttccttc
     3601 aaaccttgcg gcaatgcggc aaatactgcc tgcccggtcg gatagtggta ataacgcgac
     3661 gtaaacgcca acaaatcacg ccagctttcg ggcagcagcg gttcgtccga aaaggtcgtc
     3721 tgaacgccca aaatccgcgc cgtatccata tccggcgcaa tatccgtttc ccacaccatc
     3781 ccgaccacgg ttttattgcg gaaaggcaca agcacccgcg ttcccggagg aagcggatcg
     3841 gaatgggaat aagtcaaaag gccgtctgaa agcggcacgt ttacggcgat gcggtggtag
     3901 atcatacagg caggcaaatc atttttttgc gtattttaac aggcggaagc tattttcaga
     3961 cgataaattg agcctaggcg caatgcaggc gcaacagaga tttgatacaa ttccgctttt
     4021 ccaacacgcg caatcatctt tatatgaaaa ccaagttaat caaaatcttg acccccttta
     4081 ccgtcctccc actgctggct tgcgggcaaa cgcccgtttc caatgccaac gccgaacccg
     4141 ccgtcaaagc cgagtccgcc ggcaaatccg ttgccgcctc tttgaaagcg cgtttggaaa
     4201 aaacctattc cgcccaagat ttgaaagtgt tgagcgtcag cgaaacaccg gtcaaaggca
     4261 tttacgaagt cgtcgtcagc ggcaggcaga ttatctacac cgatgccgaa ggcggctata
     4321 tgttcgtcgg cgaactcatc aacatcgaca cgcgcaaaaa cctgaccgaa gaacgcgccg
     4381 ccgatttgaa caaaatcgac ttcgcctccc tgcctttgga caaagccatc aaagaagtgc
     4441 gcggcaacgg caagctgaaa gtcgccgtct tctccgaccc cgattgtccg ttctgcaaac
     4501 gcttggaaca cgagtttgaa aaaatgaccg acgtgacggt ttacagcttt atgatgccca
     4561 ttgccggcct gcaccccgat gccgcgcgca aggcgcaaat cttatggtgt cagcccgacc
     4621 gcgccaaagc gtggacggat tggatgcgta aaggcaaatt cccggtcggc ggcagcatct
     4681 gcgacaatcc cgtcgcggaa accacttcct tgggcgaaca attcggcttc aacggcacgc
     4741 cgaccctcgt cttccccaac gggcgcagcc aaagcggcta cagcccgatg ccccaactgg
     4801 aggaaatcat ccgcaaaaat caataatcca tccgcaaaac aaaaggcttg gaaaattcca
     4861 agcctttttt gcgggaaatg ccgtctgaaa accaatccgc actgaaagtt atagtggatt
     4921 aaatttatag tggattaaat ttaaaccagt acagcaagcc aacaaacaca tcctaaccca
     4981 ttgaatgatt gaaaataata aactgaacaa tacgaaacac accgccgtca ttcccacgca
     5041 agtgggaatc tagaacgtag aatctaagaa accgttttgt ccgataagtt tctgctccaa
     5101 aatgaccggt cctgcagctt caaacggcat ctttgtgcca acctcaatcc tgcgccaatg
     5161 cgtctatcgg attgagtttg gctgctttat tggcaggcat aaagccgaac gcgatgccga
     5221 ttccggtcga acaggcgacc gcgccgatga cggacatggc ggaaatgtcc atcgggaagt
     5281 cggttacaaa atgattgaac acgaggctga cggcggcgga caaacccacg ccgaccaaac
     5341 cgccgatgac gcagattaac accgcctcaa tcaaaaactg ctgcaaaata ttgccgcgcc
     5401 gcgcgccgat tgccatccgt atgccgattt ctttggtgcg ctcggtaacg gacaccagca
     5461 tgatgttcat cacgccgatg ccgccgacta ccaatgaaat cagggcgatg gaggaaatca
     5521 gcagcttcat cgtaccggtg gtgctttcga ctatctgcct gatgctgtcg ctgttgttca
     5581 tgaagaaatc ttccgtgccg tgccgcgctt tgagcagatc ggtcagccct ttttcggcaa
     5641 cctgggtatt ggcattgtct ttgattttga cggtgatgga gttggtgtgg ctctcgcctg
     5701 tgatttggtg catcaccgtc gtatagggcg accaaagcat cagcacgtcg gaattgccga
     5761 aagcgttttc gtcttttttc atcacgccga tgacggtcaa ggggcgtttc ctgaacaaaa
     5821 tggttttacc caacggatcc gagtccgcaa agagtttgtc tttgacattt tggtcgatga
     5881 cgacgacctg cgcgtcttct ttcacatcgt tttcgtcaaa cagccgcccc gtttccagct
     5941 tcagtccgcg cacgtcgaaa tattgttcgc ccacgccgta caaagaagcg gtcaggtcgg
     6001 tattgcggta agtcagcgtg ccgccgctcg aagtcatggg cgtggcggaa gcaacgtagc
     6061 tttgtttggc gatgattttt gcgtcgtcta tggtcagggt tctgattttg ccgctgcgcc
     6121 tgtcgccgaa gccgcgcccg gggaagatgc tgatggtgtt cgtccccatc gaactgatgt
     6181 cttcgaggat ttttttctgc gaaccgttgc ccaatgcaac gacggagaca accgaagcga
     6241 taccgatgat gatgcccagc atcgtcagaa gcgaacgcat tttgtgcgcc aatactgctt
     6301 gcaccgacat tctgaaggct tcgacaaact ggtcgtaata aaacgaccac gaggcttttt
     6361 cccgaatcct cccgaccttg cttgcgggga tttcgggatt tttcgaggtg tcggaaatga
     6421 tttcgccgtc ccggatttcg atgacgcggt tggcgttggc ggcaatgccg gggtcgtgtg
     6481 tgaccataat gacggtatgc cccgcttcgt gcagcctgcg gatgatttcc atcacgtttt
     6541 tgccgctggc ggtatcgagc gcgccggtcg gctcgtcggc gaagatgatt tcgccgccgt
     6601 tcatcagggc acgggcgatg gagacgcgct gctgctgtcc gcccgagagt tcgccgggct
     6661 tgttgccctc tttacttgcc aaacccaaat cctgcaacaa cttctccgcc cgcgcggaac
     6721 gctctttgcc gcccataccc atatacacgg ccggcagcgc aacgttgtcc cttgcggtca
     6781 gcgagcttaa gaggttgtag cgttggaaga tgaaaccgaa gcgttcgcgg cgcaatgccg
     6841 ccagttcgtc aggctgcatt ttggcagttt cgatgccgtc gattcggtac gaaccggaac
     6901 cggcggtatc caaacaaccg agtatgttca tcagcgtgga cttgcccgaa ccggactgcc
     6961 cgatgatggc gacaaaatcg cccttctcta tcgacaggct gatgtctttc aaaatatgga
     7021 cgcggttctc gccgctgccg aaatagcggt tgatgttttt acattcgatc aagctcataa
     7081 tattccgtaa atcaataaca aatgccgtct gaaaccgttt ccgtgttcag acggcatatt
     7141 cgtttatcgg cgcggcgggc cgcctagggc gcgttcgccg ctttcctgtt gctcggcggc
     7201 ggttatttcg gagatgacca ctttgtcccc ctctttcaac ccgcttttta cttcggtatt
     7261 catactgtct ctcataccgg tccggatttc gcgttccgcc gccttgccgt ccgcacccaa
     7321 cacgcgcaca aacgccttgc cgccgcgatt tttcacggtc agcgacggaa taatcagcac
     7381 atttttcacg ccgtcgattt caaccgtatt ctgcgtcgtc atccccgtgg cgagtttgcc
     7441 gtccgaattc ggcacaaacg aacgggcata atagtagacc gcattggaag ccgtatccgt
     7501 actgctgttg tagccgcccg acgacatcgt ggtcagcccg gggtcgacgc tgtcgagctt
     7561 cgccttaatc ggcgtatccg gttcggacaa aatcgtaaac gaaatatcct gccccgcctt
     7621 caccttggta atatcgccct cggcaatctg cattttgttc aacatcatat ccagattcgc
     7681 caattggaca atcgtcggcg tagactgcgc cgcgttcaca gtctgcccct cttccacgag
     7741 aatcgccacc accgtgccgt ccatcgttgc ggtaatgcgc gtgtagccca attccgactc
     7801 ggcggtattg atggaaattt tgctctgtct gattaaagcc ttcagctcgg caacattggc
     7861 tttggcggcg gcaaacgcat cctgcgcgct ttccaaatcc tctttggaag tcgcgttttc
     7921 cttccataac gccgcctgac gcttatattt cttctccgca ctgcccaatg caatctgtgc
     7981 cgacaccagc ttcgcctgat acgtttccaa cttggatttt tccgtattga gcgtattggt
     8041 ctgcgaggtc gaattgattt ccgcaatcaa atcgcccttt ttaacctgtt gcccgagttt
     8101 gacataaagt ttcttaatct gccccgatgc ctgcgcgccg accgatacca ggttggacgg
     8161 cgaaatctcc cctgttgcag aaaccgtccg gctgatgtcg ccgcgcctga ccgtttccgt
     8221 aatataagca gcctgcggct cgggcttcag ataagaccat ccgccccaaa ccgctgccgc
     8281 cgcgaccgcc gcaacagccg cccatttcat catttttgcc atgttttcaa ttctctgtat
     8341 gcgtcaatcc cataaatcgg aagcggattc taccgcaagt ccctgcccca tccaaggcgg
     8401 caaactgccg acaataaaaa taaccatttg ttttaaatgg cttatacaat ctgctttccc
     8461 gaaaatttca atccgtttac atattccgcc atttccgata cggcgttata atgccgtcga
     8521 ccgacaaaca aatggaaaca cattgctatg aaaaacgttc aaaaaggctt tacgctgctc
     8581 gagctgatga ttgccgtcgc catcctcggc atcctgacgc ttatcaccta cccttcctat
     8641 aaaacctaca tccggcgcgt ccgcctgtcg gaagtcagga cgaccctgct gcacaacgcg
     8701 cagaccatgg aacgttacta ccgccaaaaa gggacgtttg acaactacaa ccaagccaaa
     8761 ctgaaacaaa acaaatattt caacattacc ttaagcgaag tcagccccga ccacttcacc
     8821 cttcaggcag cccccgatcc gatgaccaac gacggcgaaa cctgcgtcgt tacgctcaac
     8881 gacggcggta ccattgccgc ttccggtaca aaccaatcct gccccggctt cgactaatcc
     8941 tccgccgaag cccgcaaacc cgtaaagatg ccgtccggac acagcttcag acggtatctt
     9001 tacgggtttg cacatccggt ttttcaaact tgaaacctgc cgcccctcat ccggcaaccg
     9061 ccccgatgcc gcgcctttcc aagatgttat aatataacat ataatctata tttttcaaca
     9121 acataccact tgacctgtga gatgcggaag cgtatgctta cccttatatc ggacaacatt
     9181 ccatttacag gtttttttgc cgtaactaag gagcgcaatc atgaaaatgc aggcagttgt
     9241 tgtgaataaa aatgtagcgg gcgatgtgga aatagtcgaa cgcgaggttc gcccgttgga
     9301 atacggcgag gcgttggtcg aagtcgaata ttgcggcgtg tgccacaccg acctgcacgt
     9361 tgcggcaggc gactacggcg aaaaaccggg ccgcgtgctg ggacacgagg gcatcggttt
     9421 ggttaaagaa gttgccgacg gtgtgaaaaa tctgaaagtc ggcgaccgcg tcagcatcgc
     9481 ctggctgttc caaagctgcg gctcgtgcga atactgcaat accggccgcg aaaccctgtg
     9541 ccgctctgta ttgaacgcgg gctacaccgc cgacggcggt atggcgacct actgcatcgt
     9601 gagtgccgat tacgcggtca aagtccctga aggtttggat cctgcgcaag cttccagcat
     9661 tacttgtgcc ggcgtgacca cttataaagc cattaaagtt tccagtgttc gtccgggaca
     9721 gtggattgcc atctacggcg cgggcggttt gggcaacttg ggggtccaat acgcgaaaaa
     9781 agtatttggc gcacacgttg tcgccatcga catcaacgac gacaaactgg cgtttgccaa
     9841 agaaaccggc gcggatttgg ttgtcaacgc cgccaaagaa gacgctgcca aagtgattca
     9901 ggaaaaaacc ggcggcgcac acgctgcggt cgtaaccgcc gtatctgctg ccgcattcaa
     9961 ctctgccgtg aattgcgtcc gcgcaggcgg acgtgtggtt gccatcgggc tgccgccgga
    10021 atcgatggat ttgtccatcc cgcgtttggt tttggacggc atcgaagtgg tcggctcttt
    10081 ggtcggcacg cgcaaagatt tggaagaagc cttccaattc ggcgcggaag gtttggttgt
    10141 gccgaaagtc caactgcgtg ctttggatga agcacccgcc attttccaag aaatgcgcga
    10201 aggcaaaatc accggccgta tggtgatcga tatgaaaaaa gaatgcggct gcggccatca
    10261 ccactgattt gacgtggcag tacacatcga aatgccgtct gaacgctgtt cagacggcat
    10321 tttttatgga tttggatttg attttaatct gttctgtttg aaataccgtc tgaaaaccct
    10381 tatcgcaaca cgtcattaaa aacggcaaga ttcagccgtt ctgcaacccg ttcaaacggc
    10441 ttccgccatt tccaacgcct gcttgatgtc cacggcgacg acgcgcgata cgcctttttc
    10501 ctgcatcgtt acgccgacga gctgctctgc catttccatc gtcaggcggt tgtgggagat
    10561 gtagaggaac tgggtttgcg ccgacatttc tttgaccagg ttgcagaagc gcgaggtgtt
    10621 ggcgtcgtcc agcggggcat cgacttcgtc caaaaggcag aacggagcgg ggttgaggct
    10681 gaacagagcg aacacgaggc tcatggcggt gagggctttt tcgccgccgg agaggaggtg
    10741 gatggtgctg tttttcttgc cgggcggacg cgccataatg gacacaccgg cggtcagtag
    10801 gtcgtcgcct atcattttga gagtggcttc gccgccgccg aacagggtcg ggaagaaggt
    10861 ttggactttg ccgttgacgg cgtcgaaggt ttctttgaaa cgcgctttgg ttttgtcgtc
    10921 gatttgggcg atggcttctt ccaaaatggt gatggcggcc tgcacgtctt cgctttggct
    10981 gcggtagtag ccgtcgcgtt cgcgcgcttc ttcgagttct tgcagggcgg cgaggttgac
    11041 cgcgccgagg gcttcgattt gttgcgagag gctgccgatg ctgctgttca atactttcgg
    11101 cgattctttc gccaacgctt cgagcgcatc caaatcggcg gcgcgttcgg tcaggttttg
    11161 atggtagcgt ttggcgttga tcagggcttc ctgttgttgc aacagggcgg tttgggtggc
    11221 ggcctgaagc tgtggcagct tggtttgcag ggtttgtacg cgggcgtatt gttccctacc
    11281 ctgctcctga atctgcgcga gtttctcctg tacgacaata tattcttcgt ccaaactctg
    11341 tacggcttcg ctcaactctt cgagcttgat gtgctgctcg tcgttttgga actcggtttc
    11401 ataggcaagg gcaagctctt gctggcgttc ctgccagtct agggtttgct gttcgagtcg
    11461 ggcgatttgc tgccggtagt tttgtttttg ctggttgagc ttgtggacgg cgacttcggc
    11521 cagcccgtat tggcggttgg cttccaacag ggcaagctgc gcctgtttca gacggccttg
    11581 ctgctcttgg cggctgtgtg cggtggtctg ctgttggtgt tcgagttcgg cggcggcttc
    11641 ctgcaaagta gcgatgtcgt ctgaaagccc gtcggacgtg tgttgcaaca cggtctgctc
    11701 ttccgccaac tgcgccagtt cgcgctcgat gtgttcgcgg cggatttgcc cttggttggt
    11761 gcgcgccaag agttcggcgg cgcgttgctg cgcttggctg tattggcgcg tgtgctgctg
    11821 ttgctgctgc atcaggtttt tatgttgcac ttcggacgag cgcacggcgg cttctgcctg
    11881 tttgaacgcg gcttcgtcag cggagagttc gggggctagg ttgtccaatt cggatgcgat
    11941 gccgtcgagg cgcgcttttt gggcaatcag gctttcctgc gcgggcttgg catagagcag
    12001 gacgctgacc ttatcgacct gatgaccttc gggcgtgagc cagatttgat gtgcgcccaa
    12061 atcgctttga tgcgcgaggg catagctcaa atcgggcgcg cacaatacgc cgtcgagcca
    12121 gtggtgcaat gccgtctgaa acggcggctg cgcttggatt tggttcagca atgcctgtac
    12181 gggcagggat tttttgatgc cgcctgagag gtcgtctgaa agccatgccg cctgcccctg
    12241 cggcaaaggc tcgggcggca cgaaaccttg cggcacggcg cgggcgtgca ggcgttcggc
    12301 aagaatgacg gacaaggcgt gctgccactc ggcgggcgcg gtgatgtgtt gccacagttg
    12361 cggcgcggcg gcgtggtcgg ttgcctgcca gaaatcggcg gcttcctgct gttgcgacag
    12421 gatttgcgac aacgcctgct gctgcgcctg caaggtaatg tgttgctgct tcaggctttg
    12481 gaagcggctc gatgccgtct gaaacgcctc gcgggcggcg tgtagagctt cttcggcggc
    12541 gatgatttgt tcttcgtaat gctcttgccg gttttgcaac aaggcggcgg cttcctgcgc
    12601 ggcggcggtt tcggcttcgt cgggcaggtt cagggcttgg ttttcctgtt tcagacggcc
    12661 tttgtgctct tcgtgcccgg caatcgtttg tccggtatgg gcaagctgct gctgtttcaa
    12721 cgccagttcg cggcggatgc ggtttgcctc gtcctgctgg gtttggaagg cggcattgag
    12781 cgcggtttgg gtttcttcca attcgggcag acgctcttcg tgttcggcaa cctgcatcgc
    12841 ccactccgcc agctcggttt gcttttcttc gacgtataac tcgttttctt ccagctgcac
    12901 gcggatttgc tgctgctctt gatgaatacg ctgcatctgc gcctgcgccg cctgcttgtc
    12961 gcgttcgatg cgttggtgca ggttttgctg atggcggatt tgttcttcca aacgggcaat
    13021 ctgctcgcgc aacacaccgc gtttgttgcc cagttcgtgt accgcctgct gctgcgactg
    13081 ttcggcggtc tgcaaggcgt gtacttcgtc gtttaacgcc tgaacctgcg cggcggtttc
    13141 gtcctgctgc gcctgcaaag actgatgctg cgcggtcgcc ttgtcggcgg cggcaagcga
    13201 ttgctgccat tgggcgtaat cgagcaaatc ctgttgctgg ttcagctgcg cggtcaggga
    13261 tttgtagcgt tcggcggttt ccgcctgttt ctccagcttt tccacctgac gcgctaactc
    13321 gttctgcaaa tcgcccaaac gctgcaaatg ctcgcgcgtg tctttcagac ggccttccgt
    13381 ctccttacgg cgttccttat atttggacac gcccgccgct tcctcgatat aggcgcgcaa
    13441 ttcctccggc cgcgcttcga tgatgcgcga aatcatcccc tgctcgataa cggcataacc
    13501 gcgcgcgccc acgcccgtac ccaaaaacaa atcggtaatg tcgcggcggc gcacggtctg
    13561 attgttgatg aaataagtcg attcgccctg acgcgtcagc tgccgcttga tgctcacctc
    13621 ggcatactgc ccccacgccc cctgcaaact gtggtcgctg ttgtcaaata ccagctccac
    13681 cgaagccctc ggcgcaggac ggcgcgtcgc cgcaccgtta aaaatcacgt cctgcatact
    13741 ctcgccgcga agctgcttcg ccgaagcctc acccaacacc cagcgcaccg cgtcaatcac
    13801 attcgacttg ccgcaaccgt tcggcccgat aaccgcgaca agctgccccg gaacatgaat
    13861 cgtggtcggg tcggtaaaag atttgaagcc ggagagtttg atgtgggtca ggcgcataat
    13921 ggtcggaaaa aataaaaaag aagcgtattt taacggaaat cccgccgcac caccccatat
    13981 cttgccggca aaaccttacc gcatcccgca cccgcgatgc cgtctgaagc cttcagacgg
    14041 catttttaac gcgccgaacc ccggttccgc caatgcccga tgcttggcgc ggcatttgcc
    14101 gtattgcccc gccgccgggc gcgttataat ccgccacgac atccgcccga tttggaaacc
    14161 tgcctatgaa acccgcctat ttcatttccg acctgcattt gagcgaaaag cagcccgaac
    14221 tgaccgcgct gttgctgcgt tttttacgtt cttccgccgc caggcaggcg cgggcggttt
    14281 acattttggg cgatttgttt gatttttggg tgggcgatga cgaagtttcc gagttgaata
    14341 cttcgattgc gcgtgaaatc aggaaattgt ccgacaaagg cgttgccgtg ttcttcgtca
    14401 ggggcaaccg cgacttcctg atcggtcagg atttttgccg gcaggcgggc atgacgctgc
    14461 tgccggatta ctcggttttg gacttgttcg gctgcaaaac cctcatctgc cacggcgaca
    14521 cattatgcac cgacgacaag gcataccaac gtttccgcaa aatcgtgcat cggaagcggc
    14581 tgcaaaaact gttcctgatg ctgcccctaa agtggcgcac gcgccttgcc gccaaaatca
    14641 ggcgtgtcag caaaatggaa aaacaggtca agcccgccga tattatggat gtcaatgccg
    14701 cctttaccgc gcggcaggta cgcgccttcg gtgcggaaag gctgatacac ggacacaccc
    14761 accgcgagca tatccatcac gaaaacggct ttacccgcat cgttttgggc gactggcata
    14821 acgactatgc ttcaatcctc cgcgtggacg gggacggcgc ggtattcgtg ccgctggaaa
    14881 aatactgaaa atgccgtctg aagcctttcg ggcggctttt tttacacgcc cctttcccac
    14941 actccctccg ccgcatcatc accatcctta acccgcatta aggggcgtta tcgataaaat
    15001 gcttgaccga ttacataaga ttacgtaaag tgtgcaaacg cataacaatt ggtcttacca
    15061 atttatcttt taaccaaaat atcgggtgta cgggatttga acgtctttcc aaacctcgac
    15121 gcgccttatg atgcaaatgc ctaaaaagga ttcgttatgg cacttttcct cagcatattc
    15181 cccatcgtcc tgctgatttg gctgatggtg aaaaaaaaca gtatgccctc ctacgtcgcg
    15241 ctgccgatta ccgcagtgct gatttacgcc atcaaacttt tctacttcga cgatgcgggc
    15301 atgctgctca acgccaccgc cgcttccggc ctcgtcaaaa cgctcacgcc gattaccgtg
    15361 attttcggcg cgattatgtt caaccgtatg atggaaacca cgggctgcat cgatgtcatc
    15421 cgcaaatggc tggcgaccat cagccccaac cccgtagcgc aactgatgat tatcggctgg
    15481 gcttttgcct ttatgattga aggcgcatcc ggcttcggca cgcctgccgc gattgccgcg
    15541 ccgattctga tgagcttggg cttcaacccg ttgaaagtgg cgattttcac tttggtgatg
    15601 aactccgtcc ccgtctcttt cggcgcggtc ggtacgccga cctggttcgg tttcgcaccg
    15661 ctgaacctga gtgccgaaga catcctcgcc atcggcaggc agaccggcgt aatgcacttc
    15721 ttcgcaggtt tcgtcatccc cgtcatcggc ttgggcttca tcgtaccttg gtctgaaatc
    15781 cgcaaaaact tgggcttcgt cgccattgcc gtcttctcct gcaccattcc ttatgtcgca
    15841 ttggcgatgg tcaacgaaga attcccgtcg ctcgtcgccg gcgcaatcgg cctgatggtg
    15901 tccgtattcg ccgccaacca aggctggggc ttgagcaaag accacgccaa agacccgaat
    15961 gccgaaaaag tgccgttcgc ccaagtcgcc aaagcactcg cccctttggg tatgctgatc
    16021 ggcatgctgg tggttacgcg catcaaacag ctcggcatca aaggcatttt gaccagcaaa
    16081 gaagaatggt tcagcttcca actgccgttt gatttgtcca aaatcaccgt cagcgactcc
    16141 ctgacgatta ccttcggcaa tattttcgga caagatgtca gcgcgtctta ccaaacgctg
    16201 tacgtcccgg cttggattcc gtttgtgctg accgtttgga tttgcatcct gctgtataaa
    16261 accaaattca aagatgcctg gacgatttat tccgtaactt tcaatcaaac caaaaaaccg
    16321 ctgcttgccc tgatgggcgc gctgattatg gttcagctga tgctggtcgg cggcgacaat
    16381 tcgatggtga aaatcatcgg taaggaattt gccgcaatgg cgggcgaaca ctgggtttat
    16441 ttctcgccgt atctgggcgc gatcggtgcg ttcttctccg gctccaacac cgtgtccaac
    16501 ctgactttcg gcccgattca gcagcaaatc gccttggata ccggcctgtc cgttaccctg
    16561 attctggcgt tgcagtccgt cggcggcgcg atgggcaata tggtgtgcct caacaacatc
    16621 atcgccgtat gtaccgtatt ggatgtgaaa aattccgaag gtgcgattat caagaaaacc
    16681 gttatcccga tggcgattta cggcgtgatt gccgtcgtcg cggcaatgat tttcttcctc
    16741 taggcgaaac gccaccatcc atagcggcag cacgcgggga acggaaaccg ccgttccccg
    16801 tctgggcggc gcgtcgggca gaatcccggt gccggaacag cccgccccgc caaacaaatg
    16861 ccgtctgaaa ccggaaaagg cttcagacgg catttttccg ccgtttgcat tcaggcgtaa
    16921 tgatgatgcg gcatcttgcc gtcaaggcgg tagcgcgtgc cgcaatacgg gcaggcaacg
    16981 ctgcccgatt ctccttcgca caaaggcaaa aacactctcg gatgcccgtt ccactgctcg
    17041 ttgtcgggtc ccgagcaata cagcggcaga ttttccggca acacggaaat ttcctgcaga
    17101 ttcagattgt ccatttgatt ttcctttgcg tggttggcgt gcgcccattt tacgccatcg
    17161 gcaggctaaa ggatattttc ggcgcaaagc cgcaatccgc tataatcccc acttttcaga
    17221 cggcatacca tgactgcgct tacccttccg gaagacatcc gccaacaaga gccatccgcc
    17281 ctgctctatc ccctcgtttc cgcctacctc gaacacaccg cccaaaccgg cgacgaatcc
    17341 ctctcctgcc tgtccgacga ccagcacacg ctgaccgcat tctgctacct cgacagccaa
    17401 gtcgaagaag gcggcttcgt gcaactcatc gcctccggtt acggcgaata tattttccgc
    17461 aacccgcttg ccgacagcct gcgccgctgg aaaatcaaag ccgtgccgaa agtcttggac
    17521 aaagccaaag ccctctacga acaacacggc aaaaccatcg aaacgctcgc cgacggaggc
    17581 gcagacatcg aatccctgcg caaacagttc cccgactttg aagaatggga cggcgcatac
    17641 tacgaagccg ccgaacaaga cctgcccctg cttgcagaac acatacagtc aaactgggaa
    17701 accttcgccc atatcgggca ggcgtgattg cgtctgtttc cagcccgtgt aaaacagcgt
    17761 aaaatcggca aacccgaatg accttccgtc cccatcaagg agcaagctat gttcttcaag
    17821 cacatcgaag ccgcccccgc cgatccgatt ctcggtttag gcgaagcgtt caaagccgaa
    17881 acccgccccg aaaaagtcaa cctcggcatc ggcgtttata aagacgcatc cggcgcgaca
    17941 cccctcgtca aagccgtcaa agaagccgaa aaacgcctgt tggaaagcga aaccaccaaa
    18001 aactacctca ccatcgacgg cgttgccgac tacaacgcgc aaacccaaat cctgctgttc
    18061 ggcaaagacc acgaaatcat cgccagccgt cgcgccaaaa cagcgcaaag cctcggcggt
    18121 acgggcgcat tgcgtattgc ggccgagttt gccaaacgcc agttgaacgc gcaaaccatc
    18181 tggatttcca atccgacttg gcccaaccac aacgccatcg ccaaagcggt cggtatccaa
    18241 gacaaacctt atcgttacta tgatgccgcc aaacacggtt tggattggga cggcatgatt
    18301 gaggacttga gccaagcgca aaaaggcgac atcgtcctgc tgcacggctg ctgccacaac
    18361 cctaccggca tcgaccctac gcccgaacaa tgggaaactc tggcaaaact ttctgccgaa
    18421 aaaggctggc tgccgctgtt tgactttgcc taccaaggct tcggcaacgg tttggaagaa
    18481 gatgcctacg gcttgcgcgt gttcttgaaa cacaatacag aattgctgat tgccagctct
    18541 tattccaaaa acttcggtat gtacaacgag cgcgtcggcg cgttcacttt ggtggccgaa
    18601 gatgaagaaa cagcagcccg cgcccacagc caagtcaaaa ccatcatccg taccttgtat
    18661 tccaacccgg cttcacacgg tgcgaacacc attgcgctgg tgttgaaaaa tgatgatttg
    18721 aaagcacaat ggattgccga actcgatgaa atgcgcggcc gcatcaaagc catgcgccaa
    18781 aaatttgtcg agttgctcaa agccaaaggt gcaagccaaa actttgattt cattatcgaa
    18841 caaaacggta tgttctcttt cagcggattg actcccgaac aagtcgaccg tttgaaaaac
    18901 gagtttgcca tttatgccgt ccgctccggc cgcatcaacg ttgccggcat taccgacgac
    18961 aacatcgatt atctgtgcga aagcatcgtg aaagtcttgt aaaacaaaag cgcatacgaa
    19021 atgccgtctg aaccttaacc agttcagacg gcatctgttt tttgttaaaa aacaataaac
    19081 cataacaatt tcaaacaagc taaaaaacat ctataaaatc aacccaatat gatgaactca
    19141 aataaaaaat acgaatatca tcattctcaa attcaaaatt tgaacgccac agtattatcc
    19201 gaagccacaa aaacctttcc gccgtcattc ccgcgcaggc gggaatccgg gaacccaatg
    19261 ctgcaagaat tgattagaaa caactgaaac cgagcaaact ggattcccgc ctgtgcggga
    19321 atgacgaaat aaggcgagta aaactgatga aacaaattga ttaaaaacaa taacttatat
    19381 tattttattt aaattttcga taaaatcaat tttccgtatt caatactgct ccaatcaatt
    19441 cccgcgcacc gtcgtccgcc aatttcttag cgacagcgcg tccgagcgcg tcggcatatt
    19501 cggcaggggc ttgcgcgtcc gcctgcaaaa tgaccgaacc gtcggggtgt ccgaccagtc
    19561 cgcgcaaggt cagcaagccg ttttcttccg tgcaatatgc ggccaaaggc acttggcagc
    19621 ttccgcccaa agcgcgcgcc aaagcgcgtt cggccgtaac gcaggcgtgt gtggtatcgt
    19681 ggtttagcgg ttttaaaacc tcatacaaat cttcgcggtg cgcggcaatt tcgataccca
    19741 atgcgccttg tccggcggca ggcaggctgt cggattccga caaaatcatg cggatgcgtc
    19801 cgtccaattc cagacgctgc aaaccggcgg cagccaagat aattgcgtcg tattcgccgt
    19861 tatcgagttt agacaaacgg gtttgcacat tgccgcgcaa aggtttgata accaaatgcg
    19921 gatagcgcgc acgcaactgg gcttcgcggc gcaggctgga tgtgcctacg acggcgcctt
    19981 tgggcatttc ttccaaacgc gcgtattggt tggacacaaa cgcgtcaaac ggattggcgc
    20041 gttcgccgat ggctgcaagc gcgaaacctt caggcaaatc catcggcaca tctttaatcg
    20101 aatgcacggc caagtcagcg cgcccgtcat ataaagcctg ttccaactct ttgacaaaca
    20161 agcccttacc gccgactttt gacaaagttt tgtccaaaat ctggtcgccg cgcgtggtca
    20221 tgcccaaaat ctcgacttcg caatcgggat acagggcttt cagacggcct tggatatgct
    20281 ttgcctgcca catagcaagc aggctttcgc ggctggcgat gacgagtttt ttcgggttca
    20341 tgttcaatgc gttcaaagaa tggaaacggc gtgaagtcta tcatattttg aacgtttctt
    20401 cagacggcac cgtcagatct atgttatatt tacacccaca aaacacgaaa cccgccacaa
    20461 tgaaacgcac caaaaaatac cctttcttaa cgttgcagcg gcaacgtttc catttgaact
    20521 ttgaaaacgc ctcttccgcc gccggcatcc cagccgaacg cgatttctac cgctgggcgt
    20581 ggtctgcctt gaaaagcgga taccgccgcg ccgacatcag cctgattctt ctggacgaag
    20641 aagaagcccg agcctacaac cgcgactacc gcggcaaaga ttacgccacc aatgtattga
    20701 gtttcgcgct caacgaaggc gaaatcctgc cctgccaagt ttcggaaaaa ctgtacggcg
    20761 atttgattat ctgcccgcaa gtggttttga aagaagccgc cgaacagggc aaaacgcccg
    20821 aacggcattt tgcccacctg accatacacg gcgttttgca cctgatgggc tacgaccaca
    20881 tcgaagacga tgaagccgaa ataatggaag ccgaagaaat ccgcctgatg ctggcggcag
    20941 gcttccccaa cccctaccaa gaggacgaat attaaaatgg acggtacgca gtcgaaaccc
    21001 aagtttttcg aacgcctgat ctcccgactt gccggcgagc ccgactccgc cgaagacgta
    21061 ttgaccctgc tgcgccaagc gcacgagcag gaagtttttg atgcggatac gcttttaagg
    21121 ttggaaaaag tcctcgattt ttccgatttg gaagtgcgcg atgccatgat tacgcgcagc
    21181 cgtatgaacg ttttaaaaga aaacgacagc atcgaacgca tcaccgccta cgttatcgat
    21241 accgcccatt cgcgcttccc cgtcatcggt gaagacaaag acgaagtttt gggtattttg
    21301 cacgccaaag acctgctcaa atatatgttc aaccccgagc agttccacct caaatcgata
    21361 ttgcgccctg ccgtcttcgt ccccgaaggc aaatcgctga ccgccctttt aaaagagttc
    21421 cgcgaacagc gcaaccatat ggcgatcgtc atcgacgaat acggcggcac gtcgggtttg
    21481 gtaacttttg aagacatcat cgagcaaatc gtcggcgaca tcgaagatga gtttgacgaa
    21541 gacgaaagcg cggacaacat ccacgccgtt tcttccgaac gctggcgcat ccacgcggct
    21601 accgaaatcg aagacatcaa caccttcttc ggtacggaat acagcagcga agaagccgac
    21661 accattggcg gcctggtcat tcaagagttg ggacatctgc ccgtgcgcgg cgaaaaagtc
    21721 attatcggca gcctgcaatt taccgtcgca cgcgccgaca accgccgcct gcatacgctg
    21781 atggcgaccc gcgtgaagta agcagagcct gcccgcaccg ccgtttctgc acagtttagg
    21841 atgacggtac ggacgttttc tgtttcaatc cgccccatcc gccaaacata aaaatgccgt
    21901 ctgaaaccgg aaaacaggtt cagacggcat ttcgcttacc cgcttatttt ttgctcaaag
    21961 tcagccccgt ccagccgaat aacagggtta aagccaaact caaatagcag aaaaaggcat
    22021 agggcagata ttcccagacc ggcacaccca gcgcatggct gataaatacg ccgcacacgc
    22081 tccacggaac aagcgggttg atgaccgtac ccgcgtcttc gagcgtacgc gagaggttgc
    22141 gggaatgtag tccgagctta tcgtaaacgg gtttgaacgt ttcacccgac aacaaaatac
    22201 tcaaatattg ctcgccgatc aggaaattaa ccccgaccga agtcatggca acgctgaatg
    22261 tggcacgtcc ggcattcgtc aaaaagctgc ggacggcatc cagcagggaa ggaatcgcgc
    22321 ccagcgcaaa cagcagcccg cccaaactca tcccgagaat cacgatggtt tgcgtgaaaa
    22381 acatactttc caagccgccg cgcgaaatga gtttggcgat gtctttaaac gcttcgcctt
    22441 cgagtttgta gccgccgtaa aaccacgcgc cgagctgacg cagatcgggc gtgctgtgca
    22501 gatacgttac ggcaacggcg gcaatgacgg taaagagcat agcgaccacg gcattgacgc
    22561 gcatcaatgc caaaacgacc aacagcgcaa acggaatcag cgaatagcag tgcaccaatc
    22621 ccgtggcttc aagctggctg cggaaggatt cgacgctgtt caaatcctgc gcggcgacgc
    22681 tgggaagaag ccacagcatc agtgccgcac tgatgagcca cgcgggaatg gtggtgtaca
    22741 tcatattttt gatgtgttca aacaggtcga taccgacaat ggacgcggaa atgcccgtcg
    22801 tatcggaaag cggggacatt ttgtcgccaa aaaatgcgcc cgaaacaatc gcgcccgccg
    22861 tcatcgccat atcggcctga aacgccgccg ccatacccat aaaggcaacg ccgacagtgg
    22921 cgcaggtggt caggctgctg ccgatggaca cgccgatgac ggaacacagc gcgaaggcgg
    22981 aaaaataaaa ataagtcggg gaaatcagtc cgaaaccgta atacatcagc gtcggaatcg
    23041 cgccgctcat catcagcgcg ctgaccatca gcccgatgaa gaaaaacagg taaatcgcgc
    23101 ccataccctg attcaacgcg cctatcatgc cctgctgcat atcgttgtat ttcaaaccgc
    23161 gcgccaagcc gtacaaaatc agcacgacga tggcggcaat aatggacata tgcggcagcc
    23221 actccaatga aatgatggta tagcccatcg cggcaatcag agcgacgacg acggcaagtg
    23281 cctcaccgcg cggcatatcg agtaaggatt tgaaagcgaa catgattctt tgcccgattt
    23341 gatataaagt taacggattc taacacacca taccgaatcc gcccgacgtt ttcagacggc
    23401 attttagcgt tttacaaaag ccggacagaa acaaaacggc aattttctca atttaaatta
    23461 atttttttga aatataattt tttatttttt tgtttgttga catataagac ttttatggat
    23521 tgaagtatta aaacctcggc acacacgccg caaacaccgg tacgcaaaaa tgccgtctga
    23581 aagatgttca gacgacattt ctcaagaaaa agattatttg tccgccctat atccgtacac
    23641 cgcaaaaaac acgatataca gataacagat gacggaaacg acaaacgaag acatcagggt
    23701 gtaagtatct gccacccagc cctgcacgac aggaacgacc gcaccgccga caatcgcggt
    23761 acacagtaca ccggaagccg cgttggtaaa ttttcccaat cctttggttg ccaaagagaa
    23821 aatcgtcggg aacataatcg agttgaaaaa accgatggca agcagcgacc acatcgccac
    23881 atccgcattg ccgctacccg tcgccatcgc gacggcaagc agtacgaccg cagccgatgc
    23941 gttaaacgcc aaataacggt tgggcgcgaa tttcgccatc accgccgaac cgaggaaacg
    24001 tccgaccatc gcgccgcccc aatagaacga caggtaatgc gcggcagaag catgatccag
    24061 ccctttcaga taacccaata cgttgaccat caacgaaccg atagacacct ccgcgccgac
    24121 atagcagaaa atgcctgccg taccgaacac gagatgtttg tattgccata cgctggtttt
    24181 gccgtcgtgg ttgtgcgcgc tttcctcggc ggcaattttg cgcgcgtcgg gcagccggat
    24241 cattttcacg aaaacggcaa ggataatcag cagccccgcc agtcccaaat agggaatctg
    24301 tacggaagaa atctgttccg ccttgctgac ggtttgggtt gcgtccgcca gaatcaggaa
    24361 cgcgccgatt tgcggcgcaa tggtcgtacc caaagcgtta aacgcctgaa ccagcgtcag
    24421 tgttgccgat tccttgccgg gtttcgccag cagggtaaca taaggattac cggcgacctg
    24481 aagcagcgtt acgccggaag ccaaaataaa caacgcgccc aaaaataccg cgtaagaatg
    24541 gctacccgca gcaggataaa acagcaggca tccgaccgcc gtcagcagaa agccgccgat
    24601 aacgccgttt ttgtagccga ctttgccgac aaaagccccc atcgggatgg acatcaccgc
    24661 ataggcggta aagaaacaga attggatcag catcgcctga acgtaagaca ggtcgaaaat
    24721 ttctttcaaa tgagggataa ggatgtcgtt catgcaggta ataaaaccca tcatgaagaa
    24781 cagcgtggtc aagacgacca atggggacgt atgattgttt tgtgattgcg cagacatgat
    24841 ttgcctttcg tggtgtcgga atattacccg tttcaagaaa atagaaattg acatttctta
    24901 acggcaatgc aaatcatact gatatttgaa cattttgcaa actttatcgg atgcaggcaa
    24961 tgcgccgatc tgaaattcaa gcagcagaaa ttaatatttt acagttgctt gcatagattt
    25021 taatagattt taaaaaattc cggcggactt gcaaaaaccg ttcccaataa aatgaaaatc
    25081 cgccgccgta tccggaaaat tcggaaatgc cgcatcgtgg catcaaaacc ggagcggaaa
    25141 gaatgtcgta tgcccgacaa aaaaggggca tacggctgtg ccgtattttt tgcaccgtcc
    25201 tccgcccttc ggcgggggcg tgaaaata
//
LOCUS       DAWFAN010000006        25210 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWFAN010000006 DAWFAN010000000
VERSION     DAWFAN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.006"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            41..454
                     /locus_tag="WGO65_000190"
     CDS             41..454
                     /locus_tag="WGO65_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0326555.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            500..1129
                     /gene="nth"
                     /locus_tag="WGO65_000191"
     CDS             500..1129
                     /gene="nth"
                     /locus_tag="WGO65_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ0326556.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPIADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1267..2766
                     /locus_tag="WGO65_000192"
     CDS             1267..2766
                     /locus_tag="WGO65_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ0326557.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEASFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            2966..4486
                     /locus_tag="WGO65_000193"
     CDS             2966..4486
                     /locus_tag="WGO65_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ0326558.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAIITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            4543..5628
                     /gene="nagZ"
                     /locus_tag="WGO65_000194"
     CDS             4543..5628
                     /gene="nagZ"
                     /locus_tag="WGO65_000194"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ0326559.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIAQLKELTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGVGGLKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5853..5929
                     /locus_tag="WGO65_000195"
     tRNA            5853..5929
                     /locus_tag="WGO65_000195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5887..5889,aa:Pro,seq:cgg)
     gene            complement(6264..6902)
                     /locus_tag="WGO65_000196"
     CDS             complement(6264..6902)
                     /locus_tag="WGO65_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ0326560.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGIPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRARHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(6899..7270)
                     /locus_tag="WGO65_000197"
     CDS             complement(6899..7270)
                     /locus_tag="WGO65_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ0326561.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QKRVQTLFSNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(7539..7961)
                     /gene="queD"
                     /locus_tag="WGO65_000198"
     CDS             complement(7539..7961)
                     /gene="queD"
                     /locus_tag="WGO65_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ0326562.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGG"
     gene            complement(7968..8486)
                     /locus_tag="WGO65_000199"
     CDS             complement(7968..8486)
                     /locus_tag="WGO65_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ0326563.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSEHTPHLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(8514..9173)
                     /gene="queC"
                     /locus_tag="WGO65_000200"
     CDS             complement(8514..9173)
                     /gene="queC"
                     /locus_tag="WGO65_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ0326564.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            9278..9703
                     /locus_tag="WGO65_000201"
     CDS             9278..9703
                     /locus_tag="WGO65_000201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326565.1"
                     /translation="MPSEHAAYADLMASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            9718..10938
                     /locus_tag="WGO65_000202"
     CDS             9718..10938
                     /locus_tag="WGO65_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ0326566.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSMMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKRQ"
     gene            complement(10965..11246)
                     /locus_tag="WGO65_000203"
     CDS             complement(10965..11246)
                     /locus_tag="WGO65_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0326567.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(11325..11459)
                     /locus_tag="WGO65_000204"
                     /pseudo
     CDS             complement(11325..11459)
                     /locus_tag="WGO65_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(11458..11916)
                     /locus_tag="WGO65_000205"
     CDS             complement(11458..11916)
                     /locus_tag="WGO65_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326568.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(12481..12684)
                     /locus_tag="WGO65_000206"
     CDS             complement(12481..12684)
                     /locus_tag="WGO65_000206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326569.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
     gene            complement(13248..14183)
                     /locus_tag="WGO65_000207"
     CDS             complement(13248..14183)
                     /locus_tag="WGO65_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0326570.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCLDSIDYVDERKGVP
                     LAAMQHIFMDVRAAASHAYLFEHDLKKFKQYAYVAGKLGVLLSVNSTDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPAAEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(14186..16324)
                     /locus_tag="WGO65_000208"
                     /pseudo
     CDS             complement(14186..16324)
                     /locus_tag="WGO65_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
     gene            complement(16431..16694)
                     /locus_tag="WGO65_000209"
     CDS             complement(16431..16694)
                     /locus_tag="WGO65_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326571.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(16713..18143)
                     /locus_tag="WGO65_000210"
                     /pseudo
     CDS             complement(16713..18143)
                     /locus_tag="WGO65_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
     gene            complement(18342..18716)
                     /locus_tag="WGO65_000211"
     CDS             complement(18342..18716)
                     /locus_tag="WGO65_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326572.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            complement(18994..19371)
                     /locus_tag="WGO65_000212"
     CDS             complement(18994..19371)
                     /locus_tag="WGO65_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326573.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            complement(19380..>25210)
                     /locus_tag="WGO65_000213"
     CDS             complement(19380..>25210)
                     /locus_tag="WGO65_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0326574.1"
                     /translation="KHSTMVAVAETANSQGKGKQAGSSVSVSPKTSGDLCVKLKTTLK
                     TLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQTPNARGLSHNRY
                     TQFDVDNKGAVLNNDRNNNPFLVKGSAQLILNEVRGTASKLNGIVTVGGQKADVIIAN
                     PNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGAAGWNDKGGADYT
                     EVLARAVALQGKLQGKNLAVSTGPQKVDYASGKISAGTAVGTKPTIALDTAALGGMYA
                     DSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADGTEASPTYLSIET
                     TEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPATTVLNAGRNLVI
                     ESKTNVNNAKGSANLSAGGHTMINDATIQAGSSVYSSTKGDTELGENTRIIAENVTVL
                     SNGSISSAAVIEAQDTAHIESGKPLSLETSTVASNIRLNNGNIKGGKQVVLMADGDIQ
                     AKASNLNASGNLYIHAGKDLDLNADKDLSTQSISLRADNTALISSNGNTLTAEKNLDI
                     QAGSLSVRQSNLQSSGGNVQMSATKGNISLNQSWINASQNIDTAALQGNIISDGLTAV
                     AEVGRVSLLANGNVDFNGLNTLIAEGDINAGSVGKGRLKMDNTDIYASAGDVKLVAGG
                     QLDLGNGTVNGGHISLDSNKGSMVVQDVHLNARASLKVDADQTLTINNSKLNSGHNTQ
                     INTNHGHMTLNQLDAHSRRHMSISAQGKGKGKDSGQILQNDQQNSKSTLAADGVLSLN
                     SSALQVLDNTTLRGGAINIKAGGGIIKRGHIDWETQDTATMRSAELKPLSGMMSIESG
                     GNNPLTVEPGNRIVSAGDLAVNHNGTFQISARAGNNGNPSAQTASVSAKGNIGIVAGE
                     VDIDAANMAAGKDLALVATKGNISLNSIRNTFSNYQLKTDKHNITQQLTDVEQELSKL
                     TSDPKYRKAQDLPQMLRRKYKRRDKVFGDSEARLRGLRAEINAADEAWAELQSPVKAL
                     LERKQLLQQALLTVSQPGSGHENQGSTLSGQNIKLLAAGGIRIQGSKVAATQQANIQA
                     AGFLPAPAAEELQEGRLQSAIDISGVFDTFEYGQQGSDKYGYAIFSRPSEISGKTGVT
                     LSAPYANENSRILLSTTDISSENGKIKIQSYGDQYYYARQSELYTFERRSYKTGKWYN
                     RKHITEVKEHKNAKPDAVNLSASQGIDIKSGGNIDAYATAFDAPKGSINIEAGRKLTL
                     YAVEELNYDKLDSQKRRRFLGISYSKAHDTTTQVMKTALPSRVVAESANLQSGWDTKL
                     QGTQFETTLGGATIRAGVGEQARADAKIILEGIKSSIHTETVSSSKSTLWQKQAGWGS
                     NIETLQLPSFTGPVAPVLTAPGGYIADIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNI
                     NWNQVQLAYDKWDYKQEGMTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGA
                     AAGTAAGTGVAAGTAATTGVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINH
                     TLKELGKSSTVRQAATAAVTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINST
                     AAASVHTAINGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGC
                     ASAVANKGKCRDGAIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVKKITAYAKLAAGT
                     VAGVTGGDVNTAAQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLK
                     DEGYNLADNLTTLFDETLDWNDAKAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYI
                     PNKGAVPNMSTYMKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLD
                     GQHKNHLEVFDKNGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
BASE COUNT         5819 a         6447 c         5971 g         6973 t
ORIGIN      
        1 caatccgtcg ccgattgtta cctgtttgag actgtttgca atgataccct cctacaccta
       61 cgccgccctc gccttttccg ccttcacttc cgccaccctc ctgcccggca catccgaagc
      121 cgcctttgcc ctgttcgtcc gccgctttcc cgaacacgca tacggcgcgt tgctgtgcgc
      181 cggccttgcc aacggattgg gcagtatggt ttcctattgg atgggacgtt tgctgccttc
      241 ccgaaaaatg ccgtctgaaa aaacactgaa tctgatgcgg cgtttcggta tttggctgct
      301 tccgtttgcc tggctgcccg tcgtcggcga cgcactgccg cttgccgccg gctggctgcg
      361 gttgaatccg tggacaagcg ggctgatgct ggttatcggc aaaacggcgc gttacgcctt
      421 cattctgtgg ggaatgcaat attacgccgc ctgaaccgtg cgttataatg ccgaccgcac
      481 aaaccgactg cacaaaaaaa tgaacagaca catccgccaa gaaattttcg aacgcttccg
      541 cgccgccaac ccccatccga ccaccgagct gaatttcaac tcccctttcg agcttttaat
      601 cgccgttctg ctttcggcgc aggcgaccga tgtcggcgta aacaaggcga cggcgaagct
      661 gtttccgatt gccgatacgc cgcaagcgat gctggatttg ggtttggacg gcgtgatgga
      721 atacacgaaa accatcgggt tgtataaaac caaatccaaa cacattatgc agacctgccg
      781 catcctgctg gaaaaataca agggcgaagt gccggaagac cgcgaggctt tggaatcctt
      841 gccgggcgtg gggcgcaaaa cggcaaacgt ggtattgaac acggcgttcg gacatcccgt
      901 tatggcagtc gatacgcata ttttccgagt gtccaaccga accaaaatcg cgccggggaa
      961 agacgtgcgc gaagtcgaag acaaactgat gcgcttcatc cccaaagaat ttctgatgga
     1021 cgcgcaccac tggctgattt tgcacggacg ctacacctgc aaggcgctca aaccgcaatg
     1081 ccaaacctgc atcatcaacg atttgtgcga atatcccgcc aaagcctgac cgccgccgcc
     1141 cttcaaaatg aaacgcttga aaattcggac ggacgttgta gaatttcaac ctatgccgtc
     1201 tgaagccgac aaaatgcgct tcagacggca ttaaagccgg taccggcatc cgacaaagga
     1261 caaaacgtgt tcaaaaaata ccaatacctc gctttggcag cactgtgtgc cgcctcgctg
     1321 gcaggctgcg acaaagccgg cagctttttc ggtgcggaca aaaaagaagc atccttcgta
     1381 gaacgcatcg aacacaccaa agacgacggc agcgtcagta tgctgctgcc cgactttgtc
     1441 caactggttc aaagcgaagg cccggcagtc gtcaatattc aggcagcccc cgccccgcgc
     1501 acccaaaacg gcagcggcaa tgccgaaacc gattccgacc cgcttgccga cagcgacccg
     1561 ttctacgaat ttttcaaacg cctcgtcccg aacatgcccg aaatccccca agaagaagca
     1621 gatgacggcg gattgaactt cggttcgggc ttcatcatca gcaaagacgg ctatattctg
     1681 accaatacgc acgtcgttac cggcatgggc agtatcaaag tcctgctcaa cgacaagcgc
     1741 gaatataccg ccaagctcat cggctcggat gtccaatccg atgtcgccct tctgaaaatc
     1801 gatgcaacgg aagagctgcc cgtcgtcaaa atcggcaatc ccaaagattt gaaaccgggc
     1861 gaatgggtcg ccgccatcgg cgcgcccttc ggcttcgaca acagcgtgac cgccggcatc
     1921 gtgtccgcca aaggcagaag cctgcccaac gaaagctaca cacctttcat ccaaaccgac
     1981 gttgccatca atccgggcaa ctccggcggc ccgctgttca acttaaaagg acaggtcgtc
     2041 ggcatcaact cgcaaatata cagccgcagc ggcggattca tgggcatttc cttcgccatc
     2101 ccgattgacg ttgccatgaa tgtcgccgaa cagctgaaaa acaccggcaa agtccaacgc
     2161 ggacaactgg gcgtgattat tcaagaagta tcctacggtt tggcacaatc gttcggtttg
     2221 gacaaagccg gcggcgcact gattgccaaa atcctgcccg gcagccccgc agaacgtgcc
     2281 ggcctgcagg cgggcgacat cgtcctcagc ctcgacggcg gagaaatacg ttcttccggc
     2341 gaccttcccg ttatggtcgg cgccattacg ccgggaaaag aagtcagcct cggcgtatgg
     2401 cgcaaaggcg aagaaatcac aatcaaagtc aagctgggca acgccgccga gcatatcggc
     2461 gcatcatcca aaacagatga agccccctac accgaacagc aatccggtac gttctcggtc
     2521 gaatccgcag gcattaccct tcagacacat accgacagca gcggcggaca cctcgtcgtc
     2581 gtacgggttt ccgacgcggc agaacgcgca ggcttgaggc gcggcgacga aatcctcgcc
     2641 gtcgggcaag tccccgtcaa tgacgaagcc agtttccgca aagctatgga caaggcaggc
     2701 aaaaacgtcc ccctgctggt catgcgccgt ggcaacacgc tgtttatcgc attaaacctg
     2761 caataaccca cataatttaa ccaaaatatt ttttaaatga taaaatgccc gtcaataccg
     2821 attaacgggc attttaaaaa atcagttaat tgatttcaaa taagtatttt ccggaacaga
     2881 aaatactttc gcccattttg ccaacaaaat ccgaaccgtt gcggacagaa accgccccat
     2941 ccggacgctt aacaccccca taaccatgca gctgatcgac tattcacatt catttttctc
     3001 ggttgtgcca ccctttttgg cactggcact tgccatcatt acccgccgcg tactgctgtc
     3061 tttaggcatc ggtattctgg tcggcgttgc ctttttggtc ggcggcaacc ccgtcgacgg
     3121 tctgacacac ctgaaagaca tggtcgtcgg cttggcttgg tcagacggcg attggtcgct
     3181 gggcaaacca aaaatcttgg ttttcctgat acttttgggt atttttactt ccctgctgac
     3241 ctactccggc agcaatcagg cgtttgccga ctgggcaaaa cggcacatta aaaaccggcg
     3301 cggcgcgaaa atgctgaccg cctgcctcgt gttcgtaacc tttatcgacg actatttcca
     3361 cagtctcgcc gtcggtgcga ttgcccgccc cgttaccgac aagtttaaag tttcccgcgc
     3421 caaactcgcc tacatcctcg actccactgc cgcgcctatg tgcgtgctga tgcccgtttc
     3481 aagctggggc gcgtcgatta tcgccacgct tgccggactg ctcgttacct acaaaatcac
     3541 cgaatacacg ccgatgggga cgtttgtcgc catgagcctg atgaactatt acgcactgtt
     3601 tgccctgatt atggtgttcg tcgtcgcatg gttttccttc gacatcggct cgatggcacg
     3661 tttcgaacaa gccgcgttga acgaagccca cgatgaaact gccgtttcag acgctaccaa
     3721 aggtcgtgtt tacgcactga ttattcccgt tttggcctta atcgcctcaa cggtttccgc
     3781 catgatctac accggcgcgc aggcaagcga aaccttcagc attttggggg catttgaaaa
     3841 cacggacgta aacacttcgc tggtattcgg cggcacttgc ggcgtccttg ccgtcgttct
     3901 ctgcacgctc ggcacgatta aaaccgccga ctatcccaaa gccgtttggc agggtgcgaa
     3961 atctatgttc ggcgcaatcg ccattttaat cctcgcttgg ctcatcagta cggttgtcgg
     4021 cgaaatgcac accggcgatt acctctccac actggttgcg ggcaacatcc atcccggctt
     4081 cctgcccgtc atcctcttcc tgctcgccag cgtgatggcg tttgccacag gcacaagctg
     4141 ggggacgttc ggcattatgc tgccgattgc cgccgccatg gcggtcaaag tcgaacccgc
     4201 gctgattatc ccgtgtatgt ccgcagtaat ggcgggggcg gtatgcggcg accactgctc
     4261 gcccatttcc gacacgacca tcctgtcgtc caccggagcg cgctgcaacc acatcgacca
     4321 cgttacctcg caactgcctt acgccttaac cgttgccgcc gccgccgcat cgggctacct
     4381 cgcattgggt ctgacaaaat ccgcgctgtt gggctttggc acgacaggca ttgtattggc
     4441 ggtgctgatt tttctgttga aagataaaaa acgcgccaac gcctgacccc tttccctgtt
     4501 cagacggcat atgccgtctg aagctttttg gagcaaaccg aaatgactgt tccccatatt
     4561 ccgcgcggcc ccgtcatggc agacattgcc gccttccgcc tgaccgaaga ggaaaagcaa
     4621 cgcctgctcg atcccgccgt aggcggcatc atcctcttcc gccgcaactt ccaaaatatc
     4681 gcacagctca aagaactcac cgccgaaatc aaagccctgc gcacacccga actcattatc
     4741 gccgtcgatc acgaaggcgg cagggtgcaa cgtttcatcg aaggcttcac ccgcctgccc
     4801 gcaatgaacg ttttagggca aatttgggac aaagacggcg cgtccgccgc cgaaaccgcc
     4861 gccggacaag tcggccgggt gttggcaacc gagctttccg cctgcggcat cgatttgtcc
     4921 ttcacgccag tcttagattt ggactgggga aactgccccg tcatcggcaa ccgcagtttc
     4981 caccacaatc ccgaagcggt cgcccacctc gccctcgccc ttcaaaaagg tttggcaaaa
     5041 ggcggtatga aatcctgcgg caaacatttt cccggacacg gatttgtcga aggagacagc
     5101 catctggtct tgccggaaga caggcgcagc ctgtccgaac tcgaagccgc cgacctcgcc
     5161 cccttccaca ttatgagccg cgaaggtatg gcggcggtta tgcccgccca cgtcgtttat
     5221 ccacaagtgg acacaaagcc cgcagggttt tccgaaatct ggctcaaaca aattttgcgc
     5281 cgcgatatcg gattcaaagg cgtgattttc tcggacgatt tgactatgga gggcgcgtgc
     5341 ggtgtcggcg gcctcaaaga acgcgcccgc atttcctttg aggcaggttg cgacatcgtc
     5401 ctcgtctgca accgtcccga ccttgtggac gaactgcgcg aagatttccg cattcctgac
     5461 aatcccgctt tggcgcaacg ttggcaatat atggccaaca cgctcggcag tgccgccgcg
     5521 caagccgtta tacagacggc ggatttccaa gcggcgcaag cctttgttgc cggtcttgcc
     5581 tcgccgcaag acacggcggg cggcgtaaaa gtcggagagg ccttttaaat cggctgcgtt
     5641 tcaacaatct gccgatttca accgtttgtc agggaaaggc aatgattttt tcggtcaggt
     5701 agaaagttat cttattgaat tacttggaga taaggatgct tatcaacact gtattcaaac
     5761 acagcccttt caatccgccc gacacgcggc aaaaaggcaa aacggtatag gcatgccgta
     5821 ttttttgcgc tataatcacg ccttacttaa ttcggaatgt agcgcagccc ggtagcgcac
     5881 ttcgttcggg acgaaggggt cggaggttcg aatcctctca ttccgaccaa atccaaaccg
     5941 cctcggcatc tattgccgga gcggttttgc cttttggcgt caacagaaaa atgccgtctg
     6001 aaacagaatg ctgtttcaga cggcatttcg ttttccctaa ccctgccatt tgcgcgcact
     6061 ggcacagagt atcggcaagg ttcaaaagac catcggaatt taggaagccg ggcttgtacc
     6121 caacggcagc atcttattca gtaaggctcc gtcctgattg ggcggtggaa atgccggcat
     6181 ttccacgcag aagcggcaca ctttccccgt atgtcaaaca tcggtaaaac cgtacgccgt
     6241 ccctttgagt tacactgctt aaactactct attcccgccc atttgtgcgt ctgcacgctc
     6301 aactgccaat gcacggatgc gtcggggcga ctgtttaaaa taccgatttg gcggatggtg
     6361 tcgtagatgt tcatcgcacc gtcttgctca cagggcgaaa ggtagtaatg gcgcgcacgg
     6421 attttgcgtt ccatattttc gcagaacgca acgacatcgc cgtctgccac gatgcgcact
     6481 tcgtcggctg tttcgataca gcttttttcg tatttggcgg catagcaggc tttggggctg
     6541 gtggcgacat agtcgatttg cggcggcgcg ggattgagtc cgttggtttc gatacagagg
     6601 aaataacctt ccgccttgag catgtccagc agcatatcga gatgcggctg gatggtcggt
     6661 tcgccgccgg tgatgatgat gttgcgggcg gcataggttt tcagacagcc taagatgtcg
     6721 gacaggctca tcatgccgaa tgtcaaataa tcggtatcac accagccgca ggcgaggttg
     6781 catttgccca agcggacgaa aacggcgggt atgcccgtgt tccagccttc gccttgcagg
     6841 ctttcaaaga tttcgacgat gcggtattgg ggattttcgg gggcaacatt gatttttttc
     6901 ataccgccaa caccgccgcc gctgccaaaa atgcgggcaa accttgtttg acgagtatgc
     6961 ctttgttgcc ggaagagaac gcgccccacg cggcggcaat caggacgaag ccgagaaaca
     7021 gtaccgtcgc gccgtacacc gcgttatccg gcgcggcaaa ccgcgaccac accagcccga
     7081 tgccgagaaa gccgttgtac agcccttggt tactgaacag ggtctgcacg cgcttttgtt
     7141 ccataaattc ataaggcagc ttgaacgttt ccgccgcttt ttcgctggga atctgtgtca
     7201 tttcaagcca ggcgatgtag aaatgttcga cggcgacgaa gaggactagg aggatgaaga
     7261 ggagtttcat gggtattcct tttaatgggt gtatttgata tggaaggtcg tctgaaaaaa
     7321 cggggaatcc gtacaggaga aaaaaccgtt ttacggtaaa ttcaaataag gacgctctaa
     7381 accgtccttt ccgcgcaggc gggaatccag agagttggaa ttgctgcaat ttttaaatat
     7441 tcctgaaaaa ccaaggtctg gattcccgcc tgcgcgggaa tgacggctta gggcaagttt
     7501 tattcaaatt cactatatcg atacgttcaa actatcccct accccccttc atactccgca
     7561 cacgatgtcg gcgtttccca caatttgacg cggcacacgt tcagccccgc gtttttcaga
     7621 cggtcgtaca tttcgaccgc catattttcg gcagtggtgc ggcagggcag gcgcagggtt
     7681 ttcatgttcc agccttccaa gagtgcggcg atttgagatt cgcgcccgtt gccgccgtgg
     7741 tagatgaagg cgtggtcgaa ggggtcggta atgtgttgtt tgacaatggc tttcaagtcg
     7801 gtaaagtcca tcaccatacc gtctttcggc ccgcctctga cggggtcgtc tgaaacggtg
     7861 atttcgagtt tgtaggtatg cccgtgcagg ttttggcatt tgccgtcatg cccgtcgagc
     7921 atatgcgagg agtcgaaggt gaagattttg gtgattttca tgggttctca aatgggcaaa
     7981 tagccttgaa agccgatttc ggcgggtttg ccgtgcggat tcggggtcag gcggatatgg
     8041 aaattgccga tgcggtcgag cgcaagcagg ttgtccacgt cttttaagtt gtctttatgt
     8101 tgctgaacat agctgtcggt caacagggtt tgcagggctt tttgtttggc ttcggcaggc
     8161 gtggcggcgg cgaacagccc aaaagcgtgc gcttcggcga gctgccctgc gcgataaccg
     8221 ccgacattgg caaaatacag gtgcggagtg tgttcagacg gcattttcgt tttgggcgtt
     8281 tcggatacgg cgatgtcgta accgtccgcc cattcgacaa cctgccagcc gtcgatgtgg
     8341 attttgtccg catcgccgaa ccacgcggct ttgagtgcgg ggacggcttc acggtagtcg
     8401 tcgcacacgg caaattggat gtcgtgcact tcgatattcg acctgccggc attgccgccg
     8461 aggtaaaaca tatggagctt gggcatatcg tattccttat ggtttgcgcg tgttcagacg
     8521 gcctttttac tttccagata ttccgccagc ccgcgttcgc gcaagataca gctcgggcat
     8581 tcgcggcagc cgccgacgat gccgttatag caggtgtggg tttgctcgcg gatatagtcc
     8641 agcacgccca tttcgtccgc caacgcccac gtttgcgcct tggtcagata catcagcggc
     8701 gtgtggattt gaaaatcata gtccatcgcc aaattaaggg taacgttcat cgatttgaca
     8761 aacacgtcgc ggcagtcggg atagccggag aagtcggttt cgcacacgcc cgcgatgatg
     8821 tgccgtatcc cctgcccttt ggcgtaaatc gcggcgtaaa gcaggaaaag cgcgttgcgg
     8881 ccgtctacaa aggtattcgg aacgccgttt tcggcagttt cgatggcggc ggtgtcgtcc
     8941 atcagggcat tgtgcgtaat ctgccgcatc aggctcaagt cgagtacggt ttgtttgacg
     9001 cccaaatcct gcgcaatcca gcgggcacgt tccagctcga cggcatggcg ttgcccgtat
     9061 tggaaagtaa tggcttggac gttttcgcgc ccgtagattt ggattgcctg aatcaggcag
     9121 gtggtcgaat cctgaccgcc cgaaaagatg accaaggctt tttggtttga catatcgaat
     9181 cctagttttg gtaacgcggg aggtttgcga accgcgaagt tggacagtaa agacgcggat
     9241 tatacaggca aacgcgttca gacggcatct gaacgccatg ccgtctgaac acgcggctta
     9301 tgccgacttg atggcgagtt ccgccaagcg tttgctttgt gcgaaggcga tattgttttc
     9361 ttcggcggtc aggacgggag ccgctgtcgg gaatatcttt tttgacggct tcgcagacgg
     9421 cggagacttt ggggacggtg cgcaatacgg cttcgcaacc ttcaacgctg tcgatgccgc
     9481 gagcgattcg gcgggcggga ttgcgggtgc tgccgttttg gaaatagtag aggacgagga
     9541 ttttgagggg gatttgggtt catgggcgga ttccgttaaa atggttcggt cttcagacgg
     9601 cataacagtt gttccgatgc cgtctgaaac agccgcagtt caggcatttg ttcctgacgg
     9661 caaaattggc tataacatcg ggcatcaatg cccaacttac tgaccgggca agaaattatg
     9721 acctttttac aacgtttgca aggtttggca gacaataaaa tctgtgcgtt tgcatggttc
     9781 gtcgtccgcc gctttgatga agaacgcgta ccgcaggcgg cggcaagcat gacgtttacg
     9841 acgctgctgg cactcgtccc cgtgctgacc gtgatggtgg cggtcgcttc gattttcccc
     9901 gtgttcgacc gctggtcgga ttcgttcgtc tccttcgtca accaaaccat tgtgccgcag
     9961 ggcgcggaca tggtgttcga ctatatcaat gcgttccgcg agcaggcgaa ccggctgacg
    10021 gcaatcggca gcatgatgct ggtcgttacc tcgctgatgc tgattcggac gatagacaat
    10081 acgttcaacc gcatctggcg ggtcaattcc cagcgtccgt ggatgatgca gtttctcgtc
    10141 tattgggctt tactgacgtt cgggccgctg tctttgggcg tgggcatttc ctttatggtc
    10201 ggctcggtac aggatgccgc gcttgcctca ggtgcgccgc agtggtcggg cgcgttgcga
    10261 acggcggcga cgctgacctt catgacgctt ttgctgtggg ggctgtaccg cttcgtgcca
    10321 aaccgcttcg ttcccgcgcg gcaggcgttt gtcggggctt tggcaacagc gttttgtctg
    10381 gaaaccgcgc gctccctctt cacttggtat atgggcaatt tcgacggcta ccgctcgatt
    10441 tacggcgcgt ttgccgccgt gccgtttttt ctgttgtggc tgaacctgtt gtggacgctg
    10501 gtcttgggcg gcgcggtgct gacttcttca ctctcctact ggcagggaga agcgttccgc
    10561 aggggcttcg actcgcgcgg acggtttgac gacgtgttga aaatcctgct gcttctggat
    10621 gcggcgcaaa aagaaggcaa agccttgcct gttcaggagt tcagacggca tatcaatatg
    10681 ggctacgacg agttgggcga gcttttggaa aagctggcgc ggcacggcta catctattcc
    10741 ggcagacagg gttgggtgtt gaaaacgggg gcggattcga ttgagttgaa cgaactcttc
    10801 aagctcttcg tttaccgtcc gttgcctgtg gaaagggatc atgtgaacca agctgtcgat
    10861 gcggtaatga caccgtgttt gcagactttg aacatgacgc tggcagagtt tgacgctcag
    10921 gcgaaaaaac ggcagtagtc ttgagtattt ttgaaactgt atttttatcc taaagacatt
    10981 ttcctatctg ctgattccac cgtttccttt ctgtgtgccg ccataatttt tatgatagac
    11041 aagccattca agtttgcatt gactgctttt tcattggcaa tatccaaatg gctggtcgct
    11101 gcatctaaaa ataggatttt cggttcgcaa tataaggccc gcgccaatac gatgcgttgt
    11161 ttttgtccgc ctgacagtgc gctgcccata tcgccgatca aggtttcata gcccataggc
    11221 atggcggaga tttcttcgtg aatcatagca aggcaggcgc aatgttcgat ttttcctcta
    11281 tccggttccg tatcgaaaaa acaaggggct gtactagatt agccctaaat cccacaccaa
    11341 tcccgcagga ttttaagctg ttgagacggt gtgccgaagt taaatcgaaa ttcgcattct
    11401 ttcaagaaca gcgggaaaga tttacgatcg attccgttgt attttcgcaa gacacgttta
    11461 gtctagagtc tgtatattac atttattttt agggtctgct agccaatttc ttgttccctt
    11521 cattatttta tcttctgaaa gaaaattatt tttttccatg ctattaatat taatgatatg
    11581 atttttattt aaaataattt ttccatataa aatttccggt ttgtaatagc aagagtctga
    11641 agcaaatcct tgcccgtcaa tataagaaaa ataaatgtta tcatcctcct tataatattc
    11701 taaaaccaat ccggatatta ttttatcaaa attcccttca tttccatctt tcatgactaa
    11761 aatataatta gcaggccaac ctttatcgta aacatattca aactgatagt tcccgaaact
    11821 ctcgatatcc gaactaaaaa agaagaaaag caaattaata gccaatacaa aaacaaataa
    11881 gaacaaaaat agcaaaattt tcaacttagt taacaatatt tacctctcct ttaaattcaa
    11941 tcctgaaagg taccccttac cctgggctac caattatagt ttccatattt ctaaatattg
    12001 tttttacatt acttttttct ccccccaaag gaatgcattt taaaatcatg cttttcaggt
    12061 gctaatcgat acttaccatt aacatcttta atcacagata tatttccatg tatagcccaa
    12121 cgtgaaaaat ctgagtatta tatacagtta taccttactg aaacaaagct agctgtacat
    12181 atctgtacca tatttttact acaaacaaaa aatatttata attattaagg ataagataaa
    12241 aacccccaac gccagattaa ttcttaataa tttcaaataa aaaatatttg agattttttc
    12301 ttttccaaat aatatattga aatatttaaa gttaaggtag cttgatttaa ttagaaaatc
    12361 aaaaaaaacc attaattaat ttttcttctt taaatatttt atatgatatt ataaaattaa
    12421 ataacattgc gaaaacatat gaaatcatca aaataccaaa tatatagtga gaatgcatta
    12481 ttatttagct ccttcttttg gctctgacaa attgacagtc cattgatatc caatgcttgc
    12541 atttgcaatt gctgacgcac ctgcccctgt tttccctgtt agtatcaatg gttcttttct
    12601 tgcaagatgt gcacctgcac aaccacctaa tactaaacca ccacctattg taaatccttc
    12661 ctgttgtatc tctaatgata acattttctt cttgtgccgc ccttaataca gattgtggaa
    12721 ttggattgac ctgagcagga acttctaaaa taagttttcc ttcaagctgt cttcctctta
    12781 gcagattagg tgtacttgga acattggaaa tgacaaagtg agtgcgcaaa gccatctgaa
    12841 ggcgatgtgt ttgaacctgt tgaaagcggc caacaggcta aatgcgcccg ctgccgccta
    12901 aaaggcagcc cggatgcctg atgatcgggt atccggggag gattaagggg gtatttgggt
    12961 aaaattagga gatatttggg gcgaaaacag ctgaaaacct gtgttggggt ttcggatgtc
    13021 ggggaagggc ttttttgcaa aggtctcaac tcatgttatt gcaaagatct catgccttgt
    13081 tttgccagca ggttgagttc ttctccgaca atcagcgtgg taaagccccg ccacaacaca
    13141 gatctatgta ctgtggaagg gcttcaattt atagctttcg aaaatctttc agctttaaga
    13201 cggcctaaag ttgtaatcta taaaacagga gctcagagtt ttacggctca ctctcgcttc
    13261 gcgttaccaa ataccagctt atcggttttg aacgtatagt aatctatcca atcctgcaga
    13321 tattcatacg ggtaagacaa gtcatactgt ttcataaaat cgaagatgtc ttgatattcg
    13381 gctgccggca gcggatcgta aacaatcaaa tccctcggtg cgatttcttg atctatgccc
    13441 aaatcgaaac cgtgcatgga tgcgattttg gcgtaggtaa cgatttgcgg ctgcaggtag
    13501 aaatcgaaat aggacaatgt ttcttttgca gccatacgcg cggttttttt atcgaaaagc
    13561 ggctctaagg cggctttcat tgcctcggca tcctgttcgg cgaaagcgag gaagaagcgg
    13621 taatcgtaca accgcttttg cagccatttg ctgggggtgg gatgcgccaa gactttctcg
    13681 ctacgttgtt tcaaccgatc aagctgctta ccctccacca tcagcagagt attgtaaatc
    13741 atatgacggt tgaggtcgta gcggtttata aaggcttctg tatcgttggc gatgttgtcg
    13801 atattgcgca ccagaaactc gcgcagctgc gggctgtcgc tcatcagcat cagaaacatc
    13861 ggatcttgaa tgttgagcat gtcgcaggga aagaagaagg gttcggggtc tgtagaattt
    13921 acactcagca aaactcccag ctttcctgca acataagcat attgcttgaa tttcttaaga
    13981 tcatgttcaa atagataggc atgggaagct gcagctctaa cgtccatgaa aatatgttgc
    14041 atagctgcaa gcggaacgcc ttttctttca tctacataat ctatagagtc aaggcaaccg
    14101 ctattgaaat tagcagtatt gcctatgatt acattagtaa tatgctcata ccatttttgg
    14161 gtggtcatca tattgtgccc cattgttatc tccttatatt ggctttagaa ggaactttga
    14221 caggaagaat aacggcctta cctgtttgac gatcaacgcc tgctattgct gtttttaatg
    14281 tgccgttctt atttgctttg aagacagcag ctttagcggg actaccatcg ggtaattgat
    14341 ctaaaacttg tctaatccaa tcctcactca tttgcgtata tccacccgca ccattcggat
    14401 tcagctgtac cgtaccgttc ctaatctgct tactttctac aatcaaaacg acactaccat
    14461 cggcagcctg ccatacatga tcaaaaccgt tatttccgcc gtatttgccg cccgaaagca
    14521 ctctgaagcc attttgttta gctaaagagt caaataactg ctcggtcatt ttacctgtcg
    14581 tgtcccttcc ttcgccaata gctttcagca attgtttgtc agttgctgca gtaaccctta
    14641 attcagtttg accgattttg attttggttt tactgacata gccctgtgca gtggcaacgg
    14701 ttgctgcctt gtccaaggct tttttcatgc cttgtaaatt ttttgccgct ttcgctactt
    14761 tataggcctg tatcgattca ccgatacccg gaaccacacc cagcaaagca aacaggtgat
    14821 cggcagcggt ctgtgcttgt acaaaactct gtatatcacc gataattgga ataaatcctg
    14881 tctgaagaag caaaacttct cgatattctt caggattttc acgtatccat ttttgttgtt
    14941 ccttttgaaa ttcatcacac tttgcaccaa cgcatctagg atacaaagta ttattctcca
    15001 ccgctatctg tgccgcattc gccgctgtat tcacatcccc gcctacaacg gcagacgcag
    15061 tgccggcaac cagttttgaa taggcagtaa tcttcgcttt agatttttcg atttcggtcg
    15121 cactcatacg actgaaatca gtattcttaa ccaaagcctc accaacaatc tcaccgactg
    15181 cagcgcctat cgcgccatcc tgacacttgc ccttattcgc cgccgctgcc gcacagcccg
    15241 ctatggcatg ggcaatcttg tggactatgt agtgctgatc caattgtttg attttacttg
    15301 ctgcctctcc atgcgcagta ttcaccaaag ccgcaaggat attcgcttcc agattgtctt
    15361 tcaggctgcc gccgttgaca gcggtattaa tcagtgcggc actgcccgca ttggccaggt
    15421 tgacggtcag gttgttgatc cactgcttat cgctgacatt gttcaatgcc gaagcgccga
    15481 ttttgtcggc tacgcctgcg gtagcggcgg caaccaccag atttttcacc gtgctgcttc
    15541 tgcccagctc tttcagggtg ttaccgatat tgcctttgtt gttgatgaac gatacggaag
    15601 cctggctggc caaagaggcg aatgctgcat cggttgcggc ggcggccgca ccgtttaatc
    15661 ccaatacggc tccggttcct gcgcctgagg tgaccacggt aacggccagt gcgataattg
    15721 cggctccggc tccggttagg ccttcctgtt tatagtccca tttgtcgtaa gcgagctgta
    15781 cttggttcca gttcacgtcc ttgaccgtct gaagctgttt cagataggca tattcgggct
    15841 gtttggccag cttttcgatt tcggttttga ggttgccttt ggggatgtcg gcgatatagc
    15901 cgccgggagc ggtcagctta ggcagtgccg gcccttcaaa gctcggtagc ttcagcgttt
    15961 caaccgtgct gccgcttccg gcctgctttt gccatacggt cgagttggat tccagctttt
    16021 cttcggtttg gatgcggtta acgatgcctt ttaggataat tttcgcatcg acacgggctt
    16081 tttcgcctac acctgcctga atgtcggcac cggccagcgt ggttttgaat tcggtacctt
    16141 cgagcacggt atcccagcct gaacgggtgg ctgcagtttg ggcgacgacg cggacaggca
    16201 atttggtttc gttcagttcg tttttactgt aattgctctt acctaccttg atgccgataa
    16261 agcggcggct tttttggaca tccaactcgt gcttgtgaat gccttcggct gccagcagtt
    16321 gcattgaggc cgtctgaaaa acggatattc agacggccta ttgctacttt aaataatatt
    16381 tgggtttaaa atatcatcgt atttcccaaa ttcggggaac agaaaatcaa ttatttaaat
    16441 ttttcatcct cataatatgg gtgatcaaaa agctctcctt cccacatatt tatttcttct
    16501 atgcctttat ctttcataaa ctcatgataa tcataaaaat cttttccata ttgtccgtaa
    16561 aaaattttca tatccccaag ccacatagca taacattcat cgcataaaat gacctcccca
    16621 gagaaatatt taggctttgc atgataaagg tagccttgct cacatcttgg gcaaatttta
    16681 atttctttat ccataagaga ttcctttctt aattatttct gaggatatgc agaaatgatt
    16741 ttatttgttc ctttttgcac aacaatccgc acttttgttt ctccttttgt tccaataggt
    16801 ttcttcatat ctacaagata tacggatgaa tcatttggca aaggatttcc tttcattctc
    16861 caagcttcat caaccaaagg caaaacttct ttacgggaca cattaaaaac agaatgaaca
    16921 ggtttgtttg gatttggaat tgtatgggct aaaacatgtt taatcctatt accatgctta
    16981 gcatctaacc cataatctaa ccccgccggt gttgtccaaa ccttattaac cttatcataa
    17041 gtaagcttga ttccggtctg cttctgaatt tcctgtatgg ctttagccgt atcaactgct
    17101 tcgtctcccg ccttcagaac ataagccccg cctcggctga ttttagtcgc acctgccgga
    17161 acaaagggaa cggcagctgc ggcggcatcc acaccaaaat caatcgccgc ttctcgagcc
    17221 atctgcttat cgccaataga tttggcgtaa atccatttac cgccgtccca tacgaggttg
    17281 ccgatatccc aaataatatc taatgcatta tttttaacag ctgtttcgga agcatttgcc
    17341 gccacatcaa cattgcctcc tgtaacacct acagtcgctc cggctaccaa ttttgcatac
    17401 gcagtaatat tcgctttagc tttttcaatt tcagaagcag tcataccgct gaaatcggta
    17461 ttcttaacca aagcctcccc gacaatctcc cccacgaccg caccaatcgc gccgtcctga
    17521 cacttgccct tattcgccgc cgctgccgca cagcccgcta tggcatgggc aatcttatgg
    17581 gcaatgtagt gctgatccaa ttgtttgatt ttacttgctg cctctccatg cgcagtattc
    17641 accaaagccg caaggatatt cgcttccaga ttgtctttca gactgccgcc gttaacggcg
    17701 gtgttaatca gcgcggcact gcccgcatta gccaagttga cgttgaggtt gtttacccaa
    17761 ggggtttcgc tccaagtggc aagggaggaa gcaccgattt tgttggatac gcctgcgatg
    17821 gccgctgcta caaccagttt tttcactgtg cggcttctgc ccagctcctt cagggtttta
    17881 ccgacatcgc ctttattgtt gatgagcgat acggaagcct ggctggccag cgaggcgaat
    17941 gcggcatcgg ttgccgctgc ggccgcgccg tttaagccca gtgcggctcc ggctcccgcg
    18001 cccgcagtaa ccacggtaac agccagcgca ataatcgctg ctccggctcc ggttaagcct
    18061 tcctgtttat agtcccattt gtcgtacgcc agttgtacct ggttccagtt gacgtctttg
    18121 gtgacttgga gctgtttcaa atagcaagcg tagggggtgt cttcagcacg cacgcatctg
    18181 ggcagtatat agggaatctg aatatttact tgcataacaa atgccgtctg aaaaaattga
    18241 gcttttcaga cggcattgag ccgtaaatca tggaacgcgt gcgcgctgaa gcacacacct
    18301 tacgcatgga ttttaggttt catgcaggct acagcttgct gctattcatc aaattgcggc
    18361 cattgaaagt ctgttgtttt actttcacct ctcaacagtc taatcatatc gcttttgaga
    18421 aactcaaaaa aatttttaat attaccaaca tagagcatag cttcacatag tgaactacat
    18481 gcagatttaa tgtcttcatt gtcaatagca tattgatatt ccttcatatg ctgaaaaaaa
    18541 gaatcaaagt cttcttctaa ttcatcattc caatcagatg aatagttaga aagccattgt
    18601 aagtcaagag gatcttcact attcaatttt tcagttgtgg ctttctcata aagatcaaat
    18661 ccttgtttaa ttcctaactc tcttaaactt tcttttacta cattaaaatt tttcatctga
    18721 atcaccttat ttaagattca attttcgccc ttgccctgct aatgtcttag cttaattttg
    18781 agcgagtttt aggtttcatg cagactacag cttactcagc acacacgagt ctaaacagta
    18841 tacagggaat ctaaatattt actttcataa caaatgccgt ctgaaaaaat tgagcttttc
    18901 agacggcata tggccgtaaa tcatggaacg cgtatactga agcccacacc ttatgcatgg
    18961 gttttagatt tcatgcaggc tacaacttgc tttctattca tcaagagatg gccatgaaaa
    19021 actattcttt ttatactcag cactcaataa tgttgatata tcagttttta ttgaatcaaa
    19081 tataagagat agattacctg cgtaaatcat agccataaat aaagaattac tggcaatttt
    19141 gaaattttta tcgtttaggg ctaattggca tacttccata taatctaaaa agttttttaa
    19201 atcctcctta aattcattat cccaattccc gtctgaagta taatctttaa tccatttcat
    19261 attagctgtt tcatgattaa cttcttctga tacttttgga tctgtcaaat caaacccttg
    19321 ataaagcccc acattaatca agattttttt tatatcattc aatgtttcca taaaatttcc
    19381 tattttaagt ttaatttacg accttttgct gccccagttt tcatttggtt aagcgaacca
    19441 tccatattta gaacaaactt aaagttccca tttttatcaa aaacctctaa atgattttta
    19501 tgttggccat ctaaataaaa cctatcaccg gtttttaata acccttggtt tctttttacc
    19561 aagaagacag actgcccttg ctgcgtcgga agcgttgtct tttctgaaat ttgagccagc
    19621 tgttttccaa aaggattatt tttcatgtat gtactcatat tcggtacagc acctttatta
    19681 gggatataag gacgattttt ttctaaaact tccttgacct tttgtgccgc ttccccttta
    19741 ttagcgcgat tcagctctgt tccgacgaca atatcaataa cggctttggc atcgttccaa
    19801 tccaatgttt cgtcgaataa ggtggtcagg ttgtcggcta aattataacc ttcgtctttc
    19861 aacgtctgtt ttaaatctct aacgttgatt ttcccgtttt ttaatccttt tctggctacc
    19921 ttataaacca cttttgcagc agttacaaca gctttaaccg cattattttc taccgcgttt
    19981 tgtgcggttt gtgcagcagt attgacatct cctcccgtta cgcctgcaac tgtacctgcc
    20041 gcaagtttgg cataggcggt aattttctta acttccagat ctaattgttc cggggtcata
    20101 tcgctaaaat cggtattttt aaccaaagct tccccaacaa tctcacccac ggctgcgcca
    20161 atcgcgccgt cccgacattt gcctttattt gctaccgccg atgcacagcc tgctacggca
    20221 tgggctatct tgtgggcaat gtagtcttcg ctgagattaa atttgatttt gctcgctact
    20281 tctccgtgta cggtactgac tatcgcaccc agtgcggcat cgcccaagtt gtctttcagg
    20341 ctgccgccgt tgatggcggt atggacactt gcggcagcgg tgctgttgat caggttagcg
    20401 gtcagtttgc ctgctgcggg actgtgaaaa tgtttgctga cggcttcggc tgcttgggtg
    20461 ttcagcccgc ttatgccctg cagtacgcct gcggttacgg cggcggtggc ggcctgtctg
    20521 acggtgctgc ttttgcccag ttctttcagg gtatggttta tgtctccttt gttgttgatg
    20581 agggaaactg cggcttggct ggcgagactg gccagtgcgg ctttgcctgc ggctgtggtg
    20641 atagctgcag ctgatgtgcc tgctgctact ccggttgtgg ctgccgttcc tgctgctact
    20701 ccagttccgg ctgccgttcc tgctgcggct cctcccgctc ctgcgcctgc cgcgcccgcc
    20761 gttccggcgg ctgccggggc ggacagtgca ccgtaggtca atacggttac gacgataacg
    20821 acgacagctg ctgctgcggg tgtcatgccc tcctgtttgt agtcccattt atcgtaagca
    20881 agctgcacct gattccagtt gatgtttttc gcaacttgaa gttgtttcaa ataagcatac
    20941 tcgggctgtt tggcgagttt ttcgatttcg gttttgaggt tgcctttggg gatgtcggcg
    21001 atatagccgc cgggagcggt cagtacgggc gcaacgggac cggtgaaact cggcaattgc
    21061 aaggtttcga tgttactgcc ccatcctgcc tgtttttgcc atagagtaga tttgctgctg
    21121 ctcacggttt ctgtgtggat gctgcttttg atcccttcga ggataatctt ggcatctgcc
    21181 cgtgcctgct cgcctacgcc tgcgcgtatg gttgcgccac ccagtgtggt ttcaaactgt
    21241 gtgccttgca gtttggtatc ccaacctgat tgcagattgg cagattctgc aactaccctt
    21301 gagggcagcg cggttttcat gacttgggtg gtggtgtcgt gtgctttgct gtagctgatg
    21361 ccaagaaatc tgcgcctttt ttggctgtca agtttgtcgt agttgagctc ttctacggca
    21421 tagagtgtca atttccgccc ggcttcgatg ttaatgctgc ctttgggggc atcgaatgcg
    21481 gtggcgtagg cgtcgatgtt gccaccagat ttgatgtcga tgccttggga tgcgctgagg
    21541 tttactgcgt cgggcttggc gtttttgtgt tctttgactt cggtaatgtg tttgcggttg
    21601 taccatttgc cggttttgta gctgcggcgt tcaaaggtat agagttcgct ctgtctcgca
    21661 tagtaatatt ggtcaccgta agattgaatt ttgattttgc cgttttccga actgatatcc
    21721 gtggtgctga gcaggatgcg actgttctca ttggcatacg gtgcagagag ggtaacacct
    21781 gtttttcctg aaatttcaga tggcctgctg aaaatggcat agccgtattt atcgctgcct
    21841 tgctgaccat attcaaaagt atcaaataca ccgctgatgt caatggctga ttgaagtcga
    21901 ccttcttgca gttcctctgc ggctggtgca ggtaaaaatc ctgcagcttg gatatttgcc
    21961 tgttgggtgg cagcaacttt ggagccctgg atacggatac cacctgcagc gagcagtttg
    22021 atattttgac cgctaagggt gctaccttgg ttttcgtgac cactacccgg ttgggaaact
    22081 gttaaaaggg cttgttgtaa caattgttta cgttcaagta acgcttttac tggggattgg
    22141 agttctgccc aagcctcatc agcagcattt atctctgctc gtaaaccacg taatctggcc
    22201 tcactatcac cgaatacttt gtcacgcctc ttatatttac gcctcaacat ttgtggaaga
    22261 tcctgtgctt tacgatattt aggatcgctc gttaacttac taagctcctg ctctacatca
    22321 gtaagttgct gtgtgatatt gtgtttatct gttttcaatt gatagttact aaacgtattc
    22381 ctaatactgt ttagtgaaat attcccttta gttgccacta aagccaaatc tttccctgcg
    22441 gccatatttg ctgcatcaat atccacctct cccgccacaa tcccgatatt gcctttagct
    22501 gaaacgctgg ctgtttgcgc actcggattg ccgttatttc ctgctctggc actgatttgg
    22561 aatgtgccgt tgtggttcac ggccaaatca cctgcagaaa cgatacggtt accgggttca
    22621 acggttaaag ggttattacc gcccgattct atcgacatca taccggacag tggtttcagt
    22681 tccgctgaac gcatagttgc cgtatcttgg gtttcccaat cgatatgccc ccgtttgatg
    22741 atgcctccgc cagctttgat gtttatcgcg ccaccgcgca gggtagtatt gtccaaaacc
    22801 tgtaatgcgc tgctgttcaa tgacaataca ccatctgccg ccaaagtact tttactgttt
    22861 tgctggtcgt tttgtaaaat ttgaccgctg tcttttcctt tgcctttacc ctgtgcactg
    22921 atactcatat gacgacgtga atgagcatca agctgattaa gcgtcatatg accatgattt
    22981 gtattaatct gggtattgtg accggaattg agcttgctgt tattaatggt taaagtttga
    23041 tccgcatcca ctttcagtga tgcgcgggca ttcaggtgta catcctgcac caccatgctg
    23101 cctttattgc tgtctaagct gatatgaccg ccgttaacgg tgccgttgcc taaatctaat
    23161 tgacctccgg caaccagttt cacatcgcct gcagaagcat aaatatcggt attgtccatt
    23221 ttcagacggc ctttaccgac tgaaccagca ttaatgtctc cttccgcaat caaggtattt
    23281 agaccgttga aatcaacatt gccgttggca agaagagata cgcgtccaac ttcggcaaca
    23341 gcagtcagac cgtctgaaat aatattgcct tgaagtgctg ccgtatcaat attctgactg
    23401 gcatttatcc aactctgatt caagctgatg ttgcctttag tcgcgctcat ctgcacatta
    23461 ccgccactgg attgcagatt actttgacgc acactcaagc tacctgcctg aatatccaga
    23521 ttcttttctg cagtcaaggt gttaccgttg ctactgatga gggcggtgtt gtctgccctc
    23581 aggctgatac tttgtgttga taagtcttta tcggcattca aatccagatc cttacctgca
    23641 tgaatataca gattgccgga tgcattcaga ttggaggctt ttgcctgaat gtcgccatca
    23701 gccatcagta ccacctgctt tccgccttta atgttaccgt tgttcaaacg gatgttggag
    23761 gcaacggtcg aggtttctaa agaaagcggt ttgcccgatt caatgtgtgc agtgtcttga
    23821 gcctcaatta cagcagcact gctaatacta ccgttagata atacggttac gttttcagca
    23881 ataatacggg tattttcacc caattcagta tcgcctttgg tggaactgta cacggaactg
    23941 cccgcttgaa tagtggcatc attgatcata gtatgaccgc cggccgacag attagccgaa
    24001 cctttggcat tgttcacatt agttttactc tcaatcacta aattgcgacc ggcatttaat
    24061 accgtagtag ctgggcgact gccgttattc tgcaccacgg ctccgttacg caagctgata
    24121 tcttctcccg tctcaataac caataagcct ttgctctcga tccgaccacc attggagata
    24181 aatgtgcctg ccgctccttt ttcggtggtt tcgatggaga gataagtcgg tgaagcttcg
    24241 gtgccgtcgg cagtggtggc gatgcggccg ctgttttcaa tgcggcctga cgaagtcaca
    24301 atcaattgct tggccgcttc gagtgtgccg gcatttttga cgcctacgcc tttttcattg
    24361 gcaatcagtg tgatgctgtc ggcgtacata ccgcccagtg cggcagtatc aagggcaata
    24421 gtcggtttcg tacccactgc cgtacctgca ctgattttgc cgctggcgta atctactttc
    24481 tggggaccgg tagaaaccgc caggttttta ccctgtaatt tcccctgcaa agcaactgca
    24541 cgagcaagta cctcggtgta gtcggctccg cctttatcat tccaacctgc tgctcctacg
    24601 gtcaatgtgc cttgacgcac atcaaatcct gtcagtgcac cgtctttgcc gatttggggc
    24661 gcaccggtag ttaagatgcc ccgaccgaca tttttaaagc cgccgccatt aacggtaatg
    24721 ccgttggggt tggcaataat cacgtcggcc ttttggccgc ctacggtaac gatgccgttg
    24781 agtttgctag ccgtaccgcg tacctcgttc aaaatcaatt gcgcactgcc tttgaccaga
    24841 aacggattat tgttacggtc gttgtttaac actgcccctt tgttgtcaac atcaaactgc
    24901 gtatagcggt tgtggcttaa cccgcgtgca ttcggagttt ggatattcac caagggggca
    24961 ccagtgttgg ttttaaggat aacgacctgc tggtttttag gtgctgattt gtcggtggtg
    25021 atttgagcat gggcaggcaa taccatactc agggaaacca aagagcagac caaagtttta
    25081 agggtggttt tgagtttgac gcaaaggtcg cctgaagttt tcggtgaaac agaaaccgaa
    25141 ctgcctgcct gtttaccttt gccctgactg ttggcagttt cggctactgc aaccatggtg
    25201 ctgtgctttt
//
LOCUS       DAWFAN010000007        24707 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWFAN010000007 DAWFAN010000000
VERSION     DAWFAN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.007"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            167..790
                     /gene="lolA"
                     /locus_tag="WGO65_000214"
     CDS             167..790
                     /gene="lolA"
                     /locus_tag="WGO65_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ0326575.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPALFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1063..2202
                     /locus_tag="WGO65_000215"
     CDS             1063..2202
                     /locus_tag="WGO65_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0326576.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAHPPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2335..3391
                     /locus_tag="WGO65_000216"
                     /pseudo
     CDS             2335..3391
                     /locus_tag="WGO65_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            3432..3962
                     /locus_tag="WGO65_000217"
     CDS             3432..3962
                     /locus_tag="WGO65_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ0326577.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(3979..4110)
                     /locus_tag="WGO65_000218"
     CDS             complement(3979..4110)
                     /locus_tag="WGO65_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ0326578.1"
                     /translation="MPSEPEKRLSDGIFACNSNAAGQNQGASDTVIGHPVRMNLGLT"
     gene            complement(4119..5714)
                     /locus_tag="WGO65_000219"
     CDS             complement(4119..5714)
                     /locus_tag="WGO65_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ0326579.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIENPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCEDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(5821..6216)
                     /gene="hisI"
                     /locus_tag="WGO65_000220"
     CDS             complement(5821..6216)
                     /gene="hisI"
                     /locus_tag="WGO65_000220"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ0326580.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(6247..7014)
                     /gene="hisF"
                     /locus_tag="WGO65_000221"
     CDS             complement(6247..7014)
                     /gene="hisF"
                     /locus_tag="WGO65_000221"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ0326581.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPGNTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(7027..7764)
                     /gene="hisA"
                     /locus_tag="WGO65_000222"
     CDS             complement(7027..7764)
                     /gene="hisA"
                     /locus_tag="WGO65_000222"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ0326582.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(7797..8435)
                     /gene="hisH"
                     /locus_tag="WGO65_000223"
     CDS             complement(7797..8435)
                     /gene="hisH"
                     /locus_tag="WGO65_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ0326583.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(8565..10067)
                     /gene="pta"
                     /locus_tag="WGO65_000224"
     CDS             complement(8565..10067)
                     /gene="pta"
                     /locus_tag="WGO65_000224"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ0326584.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGFAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(10252..11310)
                     /gene="fbpC"
                     /locus_tag="WGO65_000225"
     CDS             complement(10252..11310)
                     /gene="fbpC"
                     /locus_tag="WGO65_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ0326585.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSAAPAGTRGTL
                     LIRPEQFSLYPHSAPAAAIHAVVLKTTPKARHTEISLRAGQTVLTLNLPAAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(11331..12848)
                     /gene="fbpB"
                     /locus_tag="WGO65_000226"
     CDS             complement(11331..12848)
                     /gene="fbpB"
                     /locus_tag="WGO65_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ0326586.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(12916..13911)
                     /gene="fbpA"
                     /locus_tag="WGO65_000227"
     CDS             complement(12916..13911)
                     /gene="fbpA"
                     /locus_tag="WGO65_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ0326587.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(14162..14632)
                     /locus_tag="WGO65_000228"
     CDS             complement(14162..14632)
                     /locus_tag="WGO65_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ0326588.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGWEQELVLIW
                     RAEADIGNGGILQFVCNWSFPAAEKTCSVLKKIGAIHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(14689..16065)
                     /gene="argH"
                     /locus_tag="WGO65_000229"
     CDS             complement(14689..16065)
                     /gene="argH"
                     /locus_tag="WGO65_000229"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ0326589.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAKLLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSKLPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMSA"
     gene            complement(16084..16953)
                     /gene="galU"
                     /locus_tag="WGO65_000230"
     CDS             complement(16084..16953)
                     /gene="galU"
                     /locus_tag="WGO65_000230"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0326590.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQTEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(16981..17580)
                     /gene="rdgB"
                     /locus_tag="WGO65_000231"
     CDS             complement(16981..17580)
                     /gene="rdgB"
                     /locus_tag="WGO65_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ0326591.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFEPYGITVLPQSEFSIPECP
                     EPYSTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(17573..17806)
                     /locus_tag="WGO65_000232"
     CDS             complement(17573..17806)
                     /locus_tag="WGO65_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326592.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(17914..18447)
                     /locus_tag="WGO65_000233"
     CDS             complement(17914..18447)
                     /locus_tag="WGO65_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ0326593.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(18549..19007)
                     /gene="nudB"
                     /locus_tag="WGO65_000234"
     CDS             complement(18549..19007)
                     /gene="nudB"
                     /locus_tag="WGO65_000234"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ0326594.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            19218..19294
                     /locus_tag="WGO65_000235"
     tRNA            19218..19294
                     /locus_tag="WGO65_000235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19252..19254,aa:Pro,seq:ggg)
     gene            19452..21851
                     /gene="mafB"
                     /locus_tag="WGO65_000236"
     CDS             19452..21851
                     /gene="mafB"
                     /locus_tag="WGO65_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ0326595.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLVDRFHNTGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKDVKYDTRINTAVPQVNPIDEPVF
                     NPKGSVGSAHSWSITARIQYAKLPRQGRIRYIPPKNYSPSAPLPKGPNNGYSAGNLSK
                     KVLTLVYINGKLKFGPSSLPSSALKAPVPIVGKAEAPPGKAVPVGEARIPSNPKAVKG
                     TTVTLSQEQRLANGELIPKGSVVTWSDNAVKVVRPDGKKRRFNSRDWEQKALPKPQLP
                     KDYRDDINIGGNNPRFNQQRHEKIESHNPNRIEEIRQRNAVSSAERQADALKRMQTSG
                     RTRLPGQPQKGKPGAAREMSSSDNPNQTAEAFARRYLGRKPTLMERVEGEKLYGGKCV
                     GCWKAQMPDGTFVVYRPAGNASMTKGTTASVDINNPTLNNLNINLKGKPTELKFKFPA
                     R"
     gene            21862..22323
                     /locus_tag="WGO65_000237"
     CDS             21862..22323
                     /locus_tag="WGO65_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326596.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            22349..22597
                     /locus_tag="WGO65_000238"
     CDS             22349..22597
                     /locus_tag="WGO65_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0326597.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            22624..23043
                     /locus_tag="WGO65_000239"
     CDS             22624..23043
                     /locus_tag="WGO65_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326598.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            23121..23204
                     /locus_tag="WGO65_000240"
                     /pseudo
     CDS             23121..23204
                     /locus_tag="WGO65_000240"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23201..23443
                     /locus_tag="WGO65_000241"
     CDS             23201..23443
                     /locus_tag="WGO65_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ0326599.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            23521..23766
                     /locus_tag="WGO65_000242"
     CDS             23521..23766
                     /locus_tag="WGO65_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0326600.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGKDKI
                     PSYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            23769..24158
                     /locus_tag="WGO65_000243"
     CDS             23769..24158
                     /locus_tag="WGO65_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326601.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            24376..24648
                     /locus_tag="WGO65_000244"
     CDS             24376..24648
                     /locus_tag="WGO65_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326602.1"
                     /translation="MPIDNIPFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALT
                     GIAFLRGIDVYDPPVSKEKALKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT         5929 a         5832 c         6911 g         6035 t
ORIGIN      
        1 cggcacacat taaagggcag cgtggcgcgc ctgccttttc cggtgggcaa aaaatcggcc
       61 ctcggaaaac gctgtttgta aaatgcaaac cgcccgtaac gccgcccgta tgattgtttt
      121 gccgcgccga tactttacgc cacacccatc ccgacaagga aaaataatga tgaaaccgca
      181 caacctgttc caattcctcg ccgtttgctc cctgaccgtc tccgtcgctt ccgcacaggc
      241 gggcgcggtg gacgcgctca agcaattcaa caacgatgcc gacggtatca gcggcagctt
      301 cacccaaacc gtccaaagca aaaagaaaac ccaaaccgcg cacggcacgt tcaaaatcct
      361 gcgcccggcc ctcttcaaat gggaatacac ttcgccctac aaacagacta ttgtcggcga
      421 cggtcaaacc gtttggctct acgatgtcga tttggcacaa gtgaccaagt cgtcccaaga
      481 ccaggccata ggcggcagcc ccgccgccat cctgtcgaac aaaaccgccc tcgaaagcag
      541 ctacacgctg aaagaggacg gttcgtccaa cggcatcgat tatgtgctgg caacgcccaa
      601 acgcaacaat gccggctacc aatacatccg catcggcttc aaaggcggca acctcgccgc
      661 catgcagctt aaagacagct tcggcaacca aacctccatc agtttcggcg gtttgaatac
      721 caatccccaa ctctcgcgcg gcgcgttcaa gtttaccccg cccaaaggcg tggacgtgtt
      781 gagcaactga tgccgtccgc cccgatgccg tctgaaagcc gccgaggctt cagacggcat
      841 tttcacgcag gcggaacaat gtcccgcatt accgcccgat cgggcatcgg aacggcaaac
      901 ccgtgaaaat taacggttgc gccccggttg tttttgccgt ttaatgcaaa ccttgctgca
      961 ccaagggcca agaaagccga ccggccgccc ccacagcttc cgatgcaggc ggcccggccg
     1021 tccctgcaat gttttttatt tttgaacgaa aggtcgaaaa ccatgaaaaa aacactggtg
     1081 gcggcggcaa tcctgagcct cgccttgact gcgtgcggcg gcggaagcga taccgccgcc
     1141 caccccccct ccgccaagcc cgaagccgaa caatcgggca aactcaatat ctacaactgg
     1201 tcggattatg tcgatcccga aaccgtcgcc gcctttgaaa aagaaaccgg catcaagacg
     1261 cgttccgatt attacgacag caacgaaaca ctggaggcaa aagtcctgac cggcaaatcc
     1321 ggctacgacc tgaccgcgcc gtccatcgcc aacgtcggcc ggcaaatcaa agcgggcgcg
     1381 tatcagaaaa tcgacaaggc gcaaatcccc cattacggca acatcgataa agatttgctg
     1441 aaaatgatgg aagccgtcga tccgggcaac gaatacgccg tcccctattt ctggggcatc
     1501 aacaccttgg caatcaacac gcggcaggtg caaaaggcat tgggtacgga caagctgccc
     1561 gaaaacgaat gggatttggt gttcaacccc gaatacaccg ccaaactcaa atcctgcggc
     1621 atcagctatt tcgacagcgc aatcgaacag attcccttgg cgttgcacta tttgggcaaa
     1681 gaccccaaca gtgaaaatcc cgaagacatc aaagccgccg tcgatatgat gaaagccgtc
     1741 cgaggcgacg tgaaacgctt cagctcttcc ggctatatcg acgatatggc ggcgggcaac
     1801 ctgtgtgccg ccatcggtta cggcggcgat ttgaacattg ccaaaacccg tgccgaagaa
     1861 gccgcaaacg gcgtggaaat caaagtattg accccgaaaa ccggcgtggg cgtgtgggtg
     1921 gattccttta tgattccgcg cgacgcgcaa aacgtcgcca acgcccaccg ctatatcgac
     1981 tacacgctcc ggcccgaggt ggcggcgaaa aacggcagct tcgttaccta cgcgcccgcc
     2041 agccgtcccg cgcgcgagct gatggatgaa aaatacacct ccgacgcatc gattttcccg
     2101 aacaaagaac tgatggaaaa aagtttcatc gtatcgccca aatccgcaga atccgccaaa
     2161 ctgggcgtga agctgtggca agggctcaag gccggaaaat aaccgcaacc cctgccgtct
     2221 gaaacctttc gggcggcagg aaacggcgcg tccgttatca aacagggggg cgtttcccct
     2281 cctgccggtt atgattgggt taagattaaa atgatttagt aaaatgagaa agatatggat
     2341 ttaagtatcg tagttcccat ttataatgtc gaaagttatt tggaagcgtg tttaagttcc
     2401 atagaaccta tattaagtaa tgaaaatgtc gaacttatcc ttgtgaatga cgggtcaaaa
     2461 gacggaagtg aagatatatg ttataaatat atagataaaa caacaaacaa caaacaacaa
     2521 acaacaaaca acaaacaaca aacaacaaac aacaaacaac aaacaacaaa caacaaacaa
     2581 caccagatac cgaacaccaa acatcaaata tatatatcag gataaccaag ggacggcaga
     2641 agctaggaat accggaataa aaaattcaaa cggaaaatat atagtcttta ttgattcaga
     2701 tgattttatt aattgtcagg tcttgctgga ttttcttagt aaagatgata ttgatatgcc
     2761 ggatgtggtt tttttaaatg cggttaaata tgataaggga agtgtttcac attttggcga
     2821 agattatcag cctgaaaaaa tactcaagca atccaaagtc gaagttttga aaggattatg
     2881 ccgatttaga aaatttccgg gttcggcgtg taataagatt ataaaaaggg aattgattat
     2941 caaagaaaaa ctgttttttg aaaaagaggt ttatgccgaa gatatcgaat ggtcaatgag
     3001 attatttaat gcggcaacaa ctttttctta tttggacggt tgttattact attatcggca
     3061 gggaagaaaa gattctaaaa caggaactgt ttcggaaaaa agtataaagt cattattata
     3121 tattttggag aaaaatgcgg aaatggaatt tgatagggat atatcaagtt atctttattc
     3181 ttttctttcc tacgaatatc tcgttttgct ttttatcatg acgagtaaaa atatagagtg
     3241 tgatgctgat ataaaaagaa gggcgtatca tttaaggttt atgctgttaa agtccaataa
     3301 attgatatat aagctgatat ttccgataat cacattattc ggggttgata ttacaggcag
     3361 gattttaaaa gcaatcaggg ggaatattta ataaatcctt taacactata catcttaccg
     3421 aaggaggaaa aatgaacgca atccgaactt tccaaaaccg cacgcccgaa atccacgaaa
     3481 gctgtatgat agacgaagcg tgcgtcgtca tcggcgaagt gtcgcttgcc gaagacgttt
     3541 ctgtgtggcc gtgcgccgtg ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca
     3601 gcaatataca ggacggcagc gtcctgcacg tttcccacaa aaccgccgcc aaacccgaag
     3661 ggtcgccgct ggttatcggc gaagacgtta ccgtggggca caaagtgatg ctgcacggct
     3721 gccgtatcgg caaccgcgtc ctggtcggca tggggacgac ggttctggac gatgccgtga
     3781 ttgaggacga agtgatgatc ggcgcgggca gcctcgtgcc tccgcgcaaa cgtttggagg
     3841 gcggctatct ttatgtcggt tcgccggtca gacaagtgcg cgtgctgacc gatgaggaaa
     3901 aagccttttt gaaatattcc gccgcgcatt atgtgaagct gtcgaaacag tacgggatgt
     3961 gaaatcacat cggcgttcct acgtcagccc caaattcatg cggacgggat gcccgataac
     4021 ggtatccgat gcgccttgat tttgaccggc tgcgtttgag ttgcaggcaa aaatgccgtc
     4081 tgaaagcctt ttttcgggtt cagacggcat tttatcgcct aaagtttgac cgaatgttcg
     4141 cgcgtttcgt ggaacacgat gtctggccaa cgttcttgcg tcaaccccaa attcacgcgg
     4201 ttgggggcga ggtaggcgag gttgccgccc gcgtcgatgg cgaggttgcc cgcgttggct
     4261 ttttcaaatt cagccagctt tttcttgtct tcgcacgata cccagcgcgc cgaccagatg
     4321 gatgcgctgt cgaacacggc ttctacgccg tattcgttgg cgaggcgcga ggttacgact
     4381 tcaaactgca acacgccgac cgcacccaaa atcaaatccg cgccgctcat cggtttgaac
     4441 acctgtaccg caccttcttc gccaagctgt tgcaaacctt tttgcagttg cttgattttc
     4501 agcgggtttt tgatgcgaac gctgcggaac agttcgggcg cgaagaatgg gatgccggta
     4561 aacgtcagtt gttcgccttc ggagaagctg tcgccgattt ggatgttgcc gtggtttggg
     4621 ataccgataa tgtcgccggc gtaggcttct tcaaccagct cgcggtcgtg ggacatgaag
     4681 gttaccacgc tggaggcggc gatttcgcgg ttgatacgca ggtgtttcat tttcatgccg
     4741 cgctcgaatt tgccggagca gacgcgcaag aaggcaatac ggtcgcggtg tttcgggtcc
     4801 atattggctt ggattttgaa gataaatccg gaaaacttcg gctcgtccgg ctcgaccata
     4861 cgcacggtcg catcgcgtgg tttcggcgcg ggcgcccatt caatcaatga attgaggatt
     4921 tcctgaatac cgaagttgtt aatcgcagag ccgaagaata cgggcgtgag ttcgccggcg
     4981 aggaattcgt cgagattgaa ctcgttggaa gccgcctgca ccaattcgat ttcgtcgcgc
     5041 aactgttgga tttccaatgg aaagcgttgt tccaattcgg gattttctat gcctttgatg
     5101 atgtcgaact cgtgcggcag gcgttcgccg cccgcttcaa agagatagat ttcgtcgttc
     5161 aggatgtggt atacgccttt gaagtttttg cccataccga tcggccaggt aacgggcgcg
     5221 cagcggattt gcaggatgtt ttccacttcg tccaagagtt ccagcgaatc gcgcacttcg
     5281 cggtcgtatt tgttcataaa cgtaacaatc ggcgtatcgc gcaggcggca gacgtttaag
     5341 agcttgatgg tttgcgcttc cacgcctttt gccgcgtcga tgaccattaa tgcgctgtcc
     5401 acggcggtta aaacgcggta ggtgtcttcg gagaagtctt ggtgtcccgg cgtgtccaag
     5461 aggttgacgg tgtggtcttt gtagtcgaac tgcatcacgc ttgatgccac ggaaatgccg
     5521 cgctgcttct cgatttccat ccagtcggaa gtggcgaatt tgccggtttt tttgcctttt
     5581 accgtacccg cgctttgaat cgcacctgaa aacagcaaga gtttttcagt caacgtggtt
     5641 ttacctgcgt cagggtggga gatgatggca aacgtgcggc ggcggcgcac ttggtcgagg
     5701 atttcttggg acatggtttt ctttgcaaaa aggttcaggc cgcttttcag acggcccgga
     5761 cagtgtttga gacggcgaaa ttgtacaaaa aaatgcctga taattcaatg ttggaggcgg
     5821 tcagtgcgtg ctgccgtaaa tctctttttc gtctttcagg acggcatcgg cggtttccca
     5881 cgcgctgcca cgccagactt tgtaaaagca gctttctcgc ccggtgtggc aggcgatgcc
     5941 gccgttttgg gcgatgagca tcacaatggc gtcgccgtcg cagtcgaggc gcagttcgcg
     6001 gactttttgc gtgtgtcccg actcttcgcc cttcatccat tgtttttggc gcgaacggct
     6061 gtaatagtgg gcaaagccgg tttcgacggt tttttgcagg gcttcggcgt tcatccacgc
     6121 caccattaaa acacgtttgg tttcggcatc ttgggcgatg gcgcaaacca aacctttttc
     6181 gtcaaatttg acggcttcaa gcaggttttt atccatattt tctttcagac ggcatggtcg
     6241 gggcggtcag aggcgcactt cgatgccggc ttcgcgcata gcgcgtttgg cttcgcggat
     6301 ggcgatttcc ccgaaatgga aaacgccggc ggcaagtacg gcatcggctt tgccttcggt
     6361 tatgccttca atcaggtgcc ggacattgcc gaccccgccg gaggcgatga cggggatgtc
     6421 gacggcttcg gcaacggcgc gggtcagcgg caggttgaaa ccctgtttcg taccgtccct
     6481 gtccataccg gtaagcagga tttcgcccgc gccgcgtttt tgcatttcga tcgcccattc
     6541 caccgcatcc aaaccggtcg gattgcgccc gccgtgggta aagatttccc agcgtgtgtt
     6601 tccggggttg acggctttgg catcgacggc ggcgacgatg gcttgcgaac cgaaaaatcc
     6661 ggcggcttcg tcaatcaggt cgggacgggt aacggcggcg gtgttgatgc taactttgtc
     6721 cgcgccggca ttgagcaggc ggcggatgtc ggcaacggtg cgtacgccgc cgccgacggt
     6781 cagggggatg aagacttgtc cggcaacttc ttcgatgatg tgcaggatgg tgtcgcggtt
     6841 gtcggatgag gcggtgatgt cgaggaaggt caattcgtcc gcgccttcgc cgttgtagcg
     6901 tttggcggct tcgacggggt cgcccgcgtc gcgcaaaccg atgaagttca cgcctttgac
     6961 gacgcgcccg tcttttacgt cgagacaggg gatgatgcgt tttgccagtg ccataatcgg
     7021 atgcctttag tcgagggaat ctgccagttg ctgcgcttgg gcaaaatcga tgctgccctc
     7081 gtaaatcgcg cggccggtaa tcgcgcctgc tacgccatgt ttttcggcgg cacacagggc
     7141 gcggatgtcg tccaagccgg tcagtccgcc ggaggcgatg acgggaatgc ggacggcttg
     7201 ggcgagtttg accgtcgcgt cgatgttcac gccgctcatc ataccgtcgc gcccgatgtc
     7261 ggtgtagatg atgctgttga cgccgtcgtc ttcaaagcgt tttgccaaat caattacatg
     7321 atgcccggtt acggttgccc agccgtcgat ggcggccata ccgtctttgg catccagccc
     7381 gacaataatc ctgccgggga aggctttgca cgcctcgcgc acgaagtcgg ggtttttgac
     7441 cgccgccgtg ccgataatca cgtcgtttaa gcccaaatcc aaatattgtc cgatggtttt
     7501 caaatcgcgt atgccgccgc cgagctgtac ggggatgtct ttggcgacag cggcaaggat
     7561 gtctttgatg gcgggcaaat tttgcggcac gccggcaaac gcgccgttca aatctaccag
     7621 atgcaggcgg cgcgcgcctt gtttgaacca gtgcagcgcg gtttcggcgg gcgaatcgga
     7681 aaagacggtc gcctcttcca tcagcccttg tttcaggcgg acgcagcgtc cttctttcaa
     7741 atcgatggcg ggtatcagca gcatagtttt tctccttgtg cggggccgtg tccggcttac
     7801 cagtttaaaa agtttttcaa catcgtcagc ccggcatcgt ggcttttttc ggtgtgaaat
     7861 tgcgtggcga atacgttgtc tttgccgacg atgcaggcaa acggggacgg gtagtcgctt
     7921 tcgcccaata tggtttcggg attttcgggg gcgaaatagt agctgtggac gaagtaaaaa
     7981 cgcgtgtctt ggggaatatc tttaaacagc gggtggtttt gggtttggcg cacggtgttc
     8041 cagcccatat gcgggacttt cagacggcat ccctgcgggt cgcggaggtc gcgctcaaag
     8101 cgtctgactt tgccgccgaa ccagcccaag ccgtcggtgt ttccttcttc gctgtggtcg
     8161 aataaaagtt gcgcgccgac gcagattccg aaaaagggtt tgttttttaa ggcatctttg
     8221 actgcctcgt ccaggccgcc gcgcgtcagt gccgccatac agtcgggcat cgcgccctgc
     8281 ccgggaaaaa tgactttgtc ggcgcgggac acgcgttcgg ggtcgccgct taaaaagatt
     8341 tcggtatttt ttccggcaag ctgccccgcc gtccggacgg atttcaatac ggaatgcagg
     8401 ttgcccatac cgtaatcgat aatggcggtt tgcatggctt cctcctcttt tttgcaatat
     8461 ggctgcgatt ttaacaaaca aatgtgccgt gctgataaaa atgccgtctg aaaacgggag
     8521 tctgtcttca gacggcatag ggtttaaacc cggaaagccg tttgtcagcc ttccatttgt
     8581 tttgcctgaa cggcagtcag ggcgatggtg aacacgatgt cttccaccag cgcgccgcgc
     8641 gacaggtcgt tgaccggttt gcgcaggcct tgcagcagcg ggccgacgct taagacgttg
     8701 gcactgcgtt ggacggcttt ataggtgcag ttgccggtgt tcaggtcggg gaagaccaaa
     8761 acggttgcct gtcctgccac cgggctgccc ggagctttgg atttgcccac gcccggcacg
     8821 gttgccgcat catattgcag cgggccgtcg atggcgaggt cggggcgttt ttcccgggca
     8881 agtttggttg cttcgatgac ggtatcgaca tcggggccgc tgccggagtt gacggtggag
     8941 taggaaatca tcgccacttt cgggtcgatg ccgaaggctt ttgcggaatc ggcagactgg
     9001 atggcgatgt cggcaagctg ttgcgcggtc gggttcggat taaccgcgca gtcgccgaag
     9061 acgaggactt ggttgggcag cagcataaag aatacgctgg acacgaggct tgcgcccggt
     9121 gcggttttaa tcagttgcaa agcggggcgg atggtgttgg cggtggtgtg aaccgcaccg
     9181 gataccaaac cgtccacatc attttgcgcc atcatcatcg taccgagtac cacggtgtct
     9241 tgcagttgct tgcgcgcgtc ttcgggtgtc aggcctttgg atttgcgcag ttcgcacatc
     9301 ggtccgacgt attgttcgac caatgaggcg ggatcgatga tttccaaaga gtcgggcagg
     9361 ctgataccgc gttctttggc aacggcttcg acttcttcgc gtttggcaag caggacgcag
     9421 cgggcaatgc ctttttcgtg gcagatggcg gcggcttgga cggtgcgggg ttctgcgcct
     9481 tcaggcagga cgatgcgttt gtcggcttgg cgggcgaagt cgatcaggtt gtagcggaat
     9541 tgcgccggcg acaggcgttt tgcttcgcgg cctgccaata cggatacgtc tttcagcgcg
     9601 tcgctcgaac cgaagaaggt caggccggtt ttttcggctg ccgcttcggc aacggaggct
     9661 gccgcgccgt ccacgacaaa accttccaat acgcccggcg cggcggcgaa gaactgtttg
     9721 gcaaggttca cccgatttgc cagttcgtcg gcatcggcgt tgtcggaacg gacggcgaag
     9781 acggctgccg cgtcaaggga caatgccagt tcgacgtttt tgcctgcgag gtagattttg
     9841 tcggcatcgg gcgcgaagcc ttcgatgacg aggttggcag catcgagtgc ggcaactttg
     9901 ccgaccagtg cgtcgaacca gtcgtcgctt ttgccttgcg cgagcagggt ttcggcggtt
     9961 gcgtcaacgg cttggaaaat ttgtgcgtcc agtgcttttg caaaggcttg tgcggcggcg
    10021 gaggcgtcca gtccggcaga tacgggtacg atgagtactt ttgccatgat atatcctttc
    10081 gtatgctgcg gtgtgcggca tatgtggttg gaaggggcgg catataggca gaaacggctg
    10141 cctgcgtgcc gtgcgtgccg tgtttggctt gaggcgcgca ggttgaatat agcaaacaaa
    10201 ttctgtttcc aacaagataa atatccgcag gcttgtggat gctgccgcct ttcagagggt
    10261 atttccgggg aagaacaggg cgggaccgtc caaatggagg acggcggaaa tgccgtctga
    10321 cagggtaggg gcggcgggga ggttgagcgt gaggacggtt tgtccggccc ggaggctgat
    10381 ttcggtgtgc cgtgctttgg gcgtggtttt gagaaccacg gcgtgaatgg cggcggcggg
    10441 tgcggaatgg gggtaaaggc tgaactgttc cggacggatg agcagtgtgc cgcgcgtgcc
    10501 tgcgggtgcg gcgctttgga cgggcaggcg gcccaatccg caatcggcgg tgccgtcggc
    10561 gttgagcgcg gcggggaaca cgatgccttc gccgataaac agggcggcat caaggtcggc
    10621 aggttgtcgg tacaattcgt gagggcttgc agtttggagg atgcgcccct gtttcatcac
    10681 ggcaatccgg tcggcgtatt gcagggcttc ttcgcggtcg tggctgacga aaacggcaga
    10741 tttgccgttg gcgcgcaggg cggcaatcat gtcttcgcga atctggcggc gcaactgttc
    10801 gtccagcgcg ctgaagggtt cgtccaacaa aatcagttcg ggatcgggtg cgagggcgcg
    10861 ggcgagggcg acgcgctgtt gctgtccgcc cgaaagttcg tgcggatagc gtccggcaag
    10921 ttcggaaatg ccggtcaatt ccaacatagc ttcgatgcgc tgccgctctt gcgccgtctt
    10981 gcctttgccg ttgcccagcc cgtaggcggt gttgcggtaa acggtcaggt gggggaacag
    11041 cacaccttcc tgtacgacat aacccaaacg gcgttcgcgg acggggaggt tggtattttt
    11101 cgagaagatg gttctgccgg aaagcgaaat ttcgccaaaa tcgggttgtt caaaaccggc
    11161 aaggcagcgt aaaagggtgg ttttgccgca gccggacgcg ccgacgataa agaggatttc
    11221 gcccgggtcg aggctgagcg aaatgtcgtt taaaactggg gtgttttgaa aacttttgga
    11281 caggtgtccg atgtgcaggg cggcggtcat ggcggtactt cctcaagctg ttatttgaag
    11341 gcgtatttct tcagcaggaa tacggggatg ccggaaaata ataccagcat cagcgcgtaa
    11401 ggggtggcgg cggcgtattg tgcgtccgat gtgtattccc aaacggcggt ggagagtgtg
    11461 tggacatcgt cggtggtcag cagcagggtg gcggtcagct ctttcatcag tttgaggaag
    11521 acgagtgcga atgcggcggt aatgccgggc aggatggacg gcagtaccaa cgtcctgaaa
    11581 ataaagaagt gtccgcgccc caatgttgcg ccgacctgtt ccatcccttt tgggagttgt
    11641 tccaaggaag tcctcagggt ggtttgcgcc atcggcaggt aaagcatgaa ataggcaagg
    11701 atgacgacga taaaggtttg gtaaacggca ggggtgtagt tgatgctgaa ataaaccaag
    11761 gatagggcga taaccaaacc ggggacggcg tgcagtaaaa acggcagcct gtctatccaa
    11821 acggttaaaa aattgcgata gcgaaccgat gcccaaacaa ggggcaaggc acataatata
    11881 gtcaaaatcg cacctaaagc cgatacgctt aaggaacgga taaaggcatc aaatacggat
    11941 acgagcgcga atgtgccgga agtgccgacc atcatccaat gtatcaatac gccaaagggg
    12001 ataataatgc ccaaagtcaa caagctgctt aaaaaaacaa tcgcgccaat ctgaccgggc
    12061 agtttgaggg ttttgacggg ataaggacgg gcaacgcctt tgccgctgtg gtaaatcttg
    12121 gctttgccgc gaaatatgct ttctccaaat acgacgatgc cgcacaccgc cattaaaaca
    12181 gcggaaagca gggcggcggt attgttgttg taggacattt cgtattcttg gaaaatggcg
    12241 gtggtaaaag tggggtagtt caaaatagat accgcgccaa attcgaccag catatgcagg
    12301 gcaatcagta acacgctgct gccgatggcg ggtttgagct gggggaggat ggcggaaaaa
    12361 aaggtttgca ggcggctttt gcccaaggac aggctgactt cttcgtaaga caggctgatg
    12421 cgtttgagtg ccgcctcgac gggcaggtag gcgagcggga acgaggacag gctcataatc
    12481 atcactgtcc cccaaaagcc ttcgacacgg aaggtcaggc tgatccaggt gaaacagctg
    12541 acaaatgcgg ggatgcacaa aggcagggtg attgccgtct gaaaaaaggt tttgccgaag
    12601 aagcggtaac gttggaacaa aagggcgcag gcaatgccca aaacaatgga aatcagggta
    12661 acgcccgcca tcatcgtcaa ggtgttggag agcaaatccc acatacgcgg gcggaacaac
    12721 agttcgacgg cgcggttgat gccgacctgc cacgaacgca tagcgacata taaaaaaggc
    12781 agggtaagcg gtagggcaat cagtaggatg aggccggtaa gccaaatggg tattttttta
    12841 ggagacatag tgttttttat cggcaaaacg ggcggacagt ataaatgtcc acccgtttga
    12901 caatccgaaa acggcttatt tcataccggc ttgctcaagc agccgggtgg cgtgttcttt
    12961 ttcggaaaca gtggtggcgg acacttgggg tgcttccaac ttggcgatgg gttccaaatt
    13021 gaaagtggat accacgtgcg gattcaaagg atattcggca cggacggcgg tcagggcgcg
    13081 ctgtccttcc ttgctggcga ggaaggcgac gaattttttc gcctcatcct tgttttggga
    13141 ggattttaac acggctgcgc cggaataggt aacgagtgcg ccgggatctc tgtggcggac
    13201 gaaattcagg cgggtgtgga cattttgtac gcctttttca cgcgcaaaag cgtgccagta
    13261 gtagttgttg atgagggcgg catcgatttc gccgttttca accgcttgaa gggcgacgga
    13321 gtttttagcg taaggcttgc cgtattcttt cagacctttg agccatttca atgcggccgc
    13381 ttcgcctttc agtttgacga tggcgacaac ctgttccaag aacgcgccgg aagtgggggc
    13441 gtaaccgatg cggtttttcc atttcggcgt ggcgtaattc aggacggatt tttccaaatc
    13501 tttttcagac agtttgcggg tgtcgtaaac gacgacgcgc gaacgtccgc tcagtgccac
    13561 ccagtctttt ttggcggcaa ccggcacgcc cttgccgcgt gtttcgttga tggtggaggc
    13621 gggcaggggc tctaggaggt tggctgcgga aagggtggcg agtgccggga tttgttcgga
    13681 atagaatacg tcggcggggc ttcggctgcc ttcttctttg atttgaccgg caagctggtc
    13741 gcctttggcg ctgttgagtt tgactttgat gccggtagcc cgggtaaagg catctgcaac
    13801 ggcttgtgcc gcttctttgt gttggccgtt gtacacggta atgtctgcca gcgcgggggt
    13861 tgcggcggtc agggctgcgg caagcagtgc gtatcggata gatgttttca tatcgatttt
    13921 ctcctaatga atgagagtgt acaccttgtt aagacataac ggggtgtagt gtattccttc
    13981 ttctttataa atgcaaataa ttatttttta aatttgttat tatccgatcc ggttattgtt
    14041 tgttctgact tgtatttttt ccgtgcatcg cgcccgtaag gcggaagcgg cgggcaatgc
    14101 gtggcggaat gtgggtaaag gcggcatttt gatttgtcgg aatgcttgag aacccctctc
    14161 tttaaaacag cgtctgaaca gggttgccgg aatagtattg ccatcccggc agatacagtt
    14221 tgtcggggtc ttgccaatat tgttcatcca gactgttcgg cagtgaggcg gttttgttgt
    14281 cgagatgttt tgttatatcc cacatttctt tcaggttttt accttccgat tggaggtggc
    14341 ggatttcttt tctcaatgcg tctgccgccc gatggatcag cattgcgctg tgtatcgcgc
    14401 cgattttttt cagaacggaa caagtctttt cggcggcggg gaaactccag ttgcagacaa
    14461 attgcagtat gccgccgttg ccgatatcgg cttctgcccg ccaaattaaa accagctcct
    14521 gttcccaccc gtccatgctt tcgagtttgc cgtcatgctg ttcaaaaagt ttgtccaccg
    14581 cctgccacat catctgttcg aagcggtcgg cttgggtatc cgtatcggtc atagtgttct
    14641 tgtcttttaa aaatgccgtc tgaacatttc ttcagacggc atcgggggtt aagctgacat
    14701 ttcccgccag cgtttgactt ggaaacggac ttgttcaggc gcggtaccgc ccaagtggtt
    14761 gcgcgcgttt aagctgcctt cgggtgtcag cacgccgtaa acgtcgtcgg caatcaaatc
    14821 gctgaaacct tgtaagactt cgagcggcag tttgctcaaa tcgacacccg cttggtcggc
    14881 gtggcgcacg gcttgggcga cgacttcgtg ggcatcgcgg aaaggcatgc ctttttttac
    14941 caagtaatct gccaaatcgg tggcggtagc gaagccctgc atcacggcgg cgcgcatatt
    15001 gtcgggtttg acggttacgc cgcgcatcat atcggcgtaa atccgcaacg tgtcgataag
    15061 cgtgtcggcg gtgtcgaaca agggttcttt gtcttcctga ttgtctttgt tgtacgccaa
    15121 gggctgcgat ttcatcaggg taatcaagcc gataaggtgt ccgatgactc ggccggattt
    15181 gccgcgcacg agttcgggca cgtcggggtt tttcttctgc ggcatgatgg acgaacctgt
    15241 gcagaaacgg tcggcgatgt cgataaagcc gaaacgcgga ctcatccaca aaatcaattc
    15301 ttcagacagg cggctcaggt gaaccataac cagcgaggcg gcggcggtga actcgatggc
    15361 aaaatcgcgg tcggatacgg catcgagcga gttttggcag atttgttcaa agcccaacag
    15421 tttggcggtg atttcgcgcc gaatcggata agtcgtaccg gcaagggcgg ctgcgccgag
    15481 cggcatacgg ttgacgcggc ggcggcagtc cgccatccgt tcgttatcgc gtccgagcat
    15541 ttcgacgtag gcgagcatat ggtgtccgaa gctgacgggc tgggcgactt gcaggtgggt
    15601 aaagcctggc atgacggttt cggcgttttg ttccgccaaa tccagcaatg ccgtctgaag
    15661 gctttgaatc aggcttcgga tgacggtaat ctggtcgcgc agccaaaggc ggatgtcggt
    15721 ggcgacttgg tcgttgcggc tgcggccggt gtgcaggcgt ttgcccgcgt cgccgatttt
    15781 gtcggtcagg cggcgttcga tgttcatgtg gacatcttcc aaatcggacg accattcgat
    15841 tttgccgctg cggatttctt cgaggatttc cgccatgccc cgacggatgt ccgccaaatc
    15901 gccttcgtcc aacacgccgg tttctttcaa catttgcgcg tgtgccagcg agccttgaat
    15961 gtcccattcg gcaagccgtc ggtcgaaacc gatggaggcg gtgtattgtt tgacgagttc
    16021 ggaaacgggt tcgttgaaac gtccggacca ggttttgtcg tgcataagga ttccttgatg
    16081 gggttattcg gtgcggtatt tttccaaaag gcggcggaag ggttcgccgg tttcggggtg
    16141 tttcaggccg taggcgacgg tggcttcgag gtagcccagt ttgctgccgc agtcgtagcg
    16201 cgtgccttca aaggggtgcg ccaggacaaa ttcgtgatcg agcagcttgg cgatgccgtc
    16261 tgtaagctgg atttcgttgc ccgcgccgcg cggcagtccg gtcagtaagt cgaaaatgcg
    16321 cggggtaagg atgtagcgtc cgacgacggc aaggttggag ggcgcgtctt cgggcttggg
    16381 tttttcgaca atgccgataa tgcgttggaa ctgtttgagc tgttcggttt cgacgatgcc
    16441 gtatgagccg gtttgcgatg cttcaacggt ttctacgccc aaaatgctgt tgccgctgcg
    16501 cccgtacact tcgaccattt gtttgagcgc gcctttgggg gcatcaatca ggtcgtcggc
    16561 aaggataacg gcaaagggtt cgtctccgat ggcggcgcgg gcgcacaaga cggcgtgtcc
    16621 caagcccagt gcttccgtct gacggatgta gaggcaggta atgttcggcg gcaggatgtt
    16681 gcggacgtgt tccaacaatt tgtctttatg gcgcatttcc aactcggttt cgagttcgta
    16741 tgccttgtcg aaatggtctt cgatgctgcg tttgttgcgt ccggtaacaa acaccatttc
    16801 cgtgcagccg gcttccacgg cttcttctac ggcgtattgg atcagcggct tgtcgacgat
    16861 gggcagcatt tctttcgggc ttgccttggt ggcgggcagg aagcgtgttc ccatccctgc
    16921 gacggggaaa acggctttcc gtatgggttt cattcttttt cctttgtatt gttttgatgt
    16981 ttaaagggcg agtttgcgta agagttcggc aagtgcctgc gcgcggtggc tttcgcggtt
    17041 tttgacctcc gaatccaatt cggcggcggt tttgccgtgt ccgggcagat aaaaatacgg
    17101 gtcgtaaccg aaaccgtttt caccggcggg aacgccgttc cattgtccgt gccatacgcc
    17161 ttcggcaata atcggacgcg ggtcgtcttt atggcggaca aaaaccaata cgcagacgta
    17221 gcaacagctt ttgtcggctt tgccggcaag ttcggcggca agtttcaggt tgttggcggt
    17281 atcggatttg ggattgctgc cggcgtaacg tgcggaatgt atgcccggag caccgtttaa
    17341 ggcggcggca cagatgccgc tgtcgtcggc gagtgcgggc agcccgctgt gtccggcggc
    17401 gtgccgggct tttgccagcg cattttcaac aaaggtggaa tagggttcgg ggcattcggg
    17461 tatgctgaac tcggattgcg gcaatacggt aatgccgtat ggttcgaata ggttgccgaa
    17521 ttctttgagt ttgcctgcat tgccgcttgc caaaacgatt ttttccggtt tttcagacat
    17581 agcggttttc ctttgcggcg gattgggcgg cgcgtaggga tttgtgccgc aggtagaggg
    17641 cgaggctgcc gatttgtccg aacagtgcgc cgaatgcgaa aagaaaggag gcgacggcga
    17701 aggctttgct gccgacggtc agcagataag cacccaagag gacgagcagc ataaatgcca
    17761 gtgtgaagag ggcaacagac aacagataga tttttcggcg gttcatggcg ttcggtcgga
    17821 aacggtatgt tcggattata gccgattggg acggtattcc ctagagcttg gaaaaatgcc
    17881 gtctgaaacg gcgttcagac ggcatggcgg gcgttatgcc tgtttgttcc aacgttcgat
    17941 ggattctttg atgacttttt tcgcttcttc cgcatcgccc cacgattcga ctttggtcgt
    18001 acctgctttt ttcaggtctt tgtaatggtt gaagtggaac tcgatttgtt tgatgagctg
    18061 ttgcggcaaa tcggacaaag ttttgtaggc gttgccgttg ttgcggtcgt cggcaggaac
    18121 gcagacgatt ttgtcgtcta cttcgccgtc gtcaacgaat ttcatcacgc cgataacgcg
    18181 cgcttccaag aatacgccgg ttgccaaagg ttgttcggta acgagcagca cgtccaattc
    18241 gtcgccgtct tcgtccaaag tttggggaat gaagccgtag ttggtcggtt tggcgaagat
    18301 ggcgggttcg acgcggtcga gttggaatgc ggcgagtttg cggttccact cgattttgtg
    18361 gttgctgccg gcggggattt cgttgacgac gttgatgatg ccgccgtcca catcgccggg
    18421 ggtcaggatt tggttgaagt ctgccatttg gtttcctttg tttgggaaag tttgaagttt
    18481 gaaagtatag cacaaacgtc cggctgaaaa tgcgcccgat gccgtctgaa agggtgtacg
    18541 ggcgcgtgtt accgtttgcc caaaaacctg cccagttcca gaatcgcgcg cctgttggac
    18601 ggggaaaaca ctttttccgc cgcttcttcc aagccgaacc agccgtagga gacgtgttcc
    18661 tcgggttgca ggacgacggg cgtatcacgc gggatttcgg cagagaagag gtgttcgcgg
    18721 ttttcaaaca cgccttttgg atagcggtgc cgccagtggt ggtagatttc gtaaaccgtg
    18781 ctgtcgtgcc agtcttgaag ctgcccgtcc gccagcagga tgccggtttc ttcccaaact
    18841 tcgcgccttg ccgtttgtgc gacggtttcg cccggttcga ggctgccggt taccgactgc
    18901 caaaatcctt ccggatgcgt gcgttcgatg agcaggatgc cgccgtcccc gctataaagg
    18961 acgaccagtg cggaaacggg gtatttgagc ggttttgcca tcggcatctt tcggcgggct
    19021 gcggtaatga aggggcggat tatagcaaac gccgcacgtt atggcgttta tccttttccg
    19081 tatccttttt tctgcacgga tgggacgcgc cggtgtttgc cggtaaattt tccgttgtgt
    19141 caaaaagata agggcggttg tgattttaat gcttgccaaa gcgtcgggcg gaaactataa
    19201 tccgcaactt atcgagtcgg agtgtggcgc agtctggtag cgcacttgca tggggtgcaa
    19261 ggggtcgaag gttcgaatcc tttcactccg accaaaaatt ccgaaagccg ctttcaaaag
    19321 cggctttttt gccgtccgta tgattatgat gtagagtacg cggcgacaga cattcaaatg
    19381 ccgtctgaaa accgttcaga tggcatctct ttatcttagt ttcattccgt accatcttaa
    19441 ggaacatcaa attgggcatt tcccgcaaaa tatcccttat tctgtccata ctggcagtgt
    19501 gcctgccgat gcatgcacac gcctcagatt tggcaaacga ttcttttatc cggcaggttc
    19561 tcgaccgtca gcatttcgaa cccgacggga aataccacct attcggcagc aggggggaac
    19621 ttgccgagcg cagcggccat atcggattgg gaaacataca aagccatcag ttgggcaacc
    19681 tgatgattca acaggcggcc attaaaggaa atatcggcta cattgtccgc ttttccgatc
    19741 acgggcacga agtccattcc cccttcgaca accatgcctc acattccgat tctgatgaag
    19801 ccggtagtcc cgttgacgga ttcagccttt accgcatcca ttgggacgga tacgaacacc
    19861 atcccgccga cggctatgac gggccacagg gcggcggcta tcccgctccc aaaggcgcga
    19921 gggatatata cagctacgac ataaaaggcg ttgcccaaaa tatccgcctc aacctgaccg
    19981 acaaccgcag caccggacaa cggcttgtcg accgtttcca caataccggt agtatgctga
    20041 cgcaaggagt aggcgacgga ttcaaacgcg ccacccgata cagccccgag ctggacagat
    20101 cgggcaatgc cgccgaagct ttcaacggca ctgcagatat cgtcaaaaac atcatcggcg
    20161 cggcaggaga aattgttggc gcaggcgatg ccgtgcaggg tataagcgaa ggctcaaaca
    20221 ttgctgttat gcacggcttg ggtctgcttt ccaccgaaaa caagatggcg cgcatcaacg
    20281 atttggccga tatggcgcaa ctcaaagact atgccgcagc agccatccgc gattgggcag
    20341 tccaaaaccc caatgccgca caaggcatag aagccgtcag caatatcttt acggcagtca
    20401 tccccgtcaa agggattgga gctgttcggg gaaaatacgg cttgggcggc atcacggcac
    20461 atcctatcaa gcggtcgcag atgggcgcga tcgcattgcc gaaagggaaa tccgccgtca
    20521 gcgacaattt tgccgatgcg gcatacgcca aatacccgtc cccttaccat tcccgaaata
    20581 tccgttcaaa cttggagcag cgttacggca aagaaaacat cacctcctca accgtgccgc
    20641 cgtcaaacgg aaagaatgtg aaactggcaa acaaacgcca cccgaagacc aaagtgccgt
    20701 ttgacggtaa agggtttccg aattttgaaa aagacgtaaa atacgatacg agaattaata
    20761 ccgctgtacc acaagtgaat cctatagatg aacccgtctt taatcctaaa ggttctgtcg
    20821 gatcggctca ttcttggtct ataactgcca gaattcaata cgcaaaatta ccaaggcaag
    20881 gtagaatcag atatatccca cctaaaaatt actctccttc agcaccgcta ccaaaaggac
    20941 ctaataatgg atatagtgcc gggaatttaa gcaaaaaagt cctgacttta gtttacataa
    21001 acggcaaact caaattcggg ccaagcagtc tgccgtcttc ggcattgaaa gcacctgttc
    21061 ccattgtcgg caaggctgag gctccgcccg gaaaagctgt gcctgtcggc gaagcccgca
    21121 ttccttcaaa tccaaaagcg gtaaaaggaa caaccgttac cctgtcgcag gaacaacgcc
    21181 tagccaacgg agagctgata ccgaaaggct ctgtggtaac gtggtcggac aatgcggtga
    21241 aggttgtgcg gcctgatggg aaaaagcgga gatttaatag tcgggattgg gaacagaagg
    21301 cattgccgaa accgcaacta ccgaaagatt acagagatga catcaacata ggtggaaata
    21361 atcctagatt taatcagcaa cggcatgaaa aaatcgaatc ccacaaccca aacagaatag
    21421 aggagatacg ccaacgtaat gccgtttctt ctgcagaaag gcaggctgat gcattaaaaa
    21481 ggatgcaaac gtctggccgc acccgcctgc ctgggcagcc acaaaaagga aaacccggcg
    21541 ctgctagaga aatgtcctct tcagacaatc cgaatcaaac agccgaggct tttgcacgtc
    21601 gatatttagg caggaaacct acactaatgg aacgagtcga aggtgaaaaa ttatacggag
    21661 gtaaatgtgt gggctgttgg aaagcgcaaa tgccggacgg aacctttgtg gtgtataggc
    21721 cagcaggtaa tgcttctatg accaagggaa ctactgcgtc tgttgatatt aataacccaa
    21781 cattaaataa cttaaatatt aatttaaaag gcaagccaac agagttaaaa tttaaattcc
    21841 ctgctagata aggatgtttt aatgaattta gagacagcag aaaatattca atgcgaattt
    21901 gagtatttag cattagatgg ttattttcca atgcattttg catcgcatgg tcaaggaaac
    21961 aaagattggc aatttgcagt tgaattcata tatagattat taatatgtca attagcaact
    22021 cttgaaccta tcaactttga aacgaaaaat gatattttag acttttgcca taatttagca
    22081 aaacaaagtc cttttaataa tgataatgag gtgtggtatc agggggaaat tgtattaaca
    22141 aaaaaaggga tagatttaat aaaggaatat attccagagg cgtttgaaga gtggaatgga
    22201 aagaaatttg aattaaacat tcctttcatt aaaacattaa aaaatatatt tgttaattat
    22261 gaggtagcat gggatgagaa taatccacta ttccccatta tatctttaaa cctcggcacc
    22321 tgataaacat ggggtttatc aagcgacagt ggaaattaaa aagcctgatg gaagttggga
    22381 aatgaaaaaa ggaaggggcg gaaaacctat gacaaatcat tctatgttcc caaaagattg
    22441 ggatgaggct agaattaggg ctgaagttac ttcggcttgg gagaaaagaa aggaattaga
    22501 aggtcaaaaa tggagaggta caagtaaatc tggtgttgaa atagaaggat ttacggaacc
    22561 taatcgaaca gcttatccac tttataatag taaatgaagt attgattaaa aaggtgtttt
    22621 taaatgtata aaataaaatg tttttatgat cagtttagtt ctggtgaatt atttaactac
    22681 tgccagtttt tagataattc ttctaatcaa aagataaata cacgaggaac ggtgtatcaa
    22741 tatattattt atgttttaac aggagacctt tatctacaaa aagacataga tgaaaattta
    22801 gaatttatcc atcaagcaga aaataatccc aatgaagttt attcaggagg tggacaaggt
    22861 ttttgctggg atatttctgc tgaaaaagta gttttctatc acaacgaatt tgatgaagaa
    22921 gacggctggc ctgatttaag ctgttcgctg catactttta aaactgcttt aattgcttgg
    22981 aatgcttttt tgcaattgcc taaaagtatt cattcggtgg tggagactgt gattgaggaa
    23041 taagcataat tagcttaatg aatagaatca gcgatataga ttggactgca aatccacgct
    23101 tatacgctgt gccatgatta acaggaagag agcaacttgg atgggctagt agggatggta
    23161 agcatttaaa tatatcaatt gatggaaaga ttacacacaa atgactaatt taaaattaga
    23221 tttctactct gaagttatta taaaagattc ttgccctaat gatttgttag aaaatggtga
    23281 gactattaaa ggaaaaaaag gagtggtatt aggtataagc gaagaagatg gtataatcta
    23341 tggctatact attttacttt ttgatataaa atattgtata tatatagata agaaatatat
    23401 tataccgact ggaaaaaaat tctctcgcga tgatttttat tagctaattt aagtaattta
    23461 agcaagtttt ttagatactg aaactggtaa ttatgtttat gtaagcataa ggagttcaga
    23521 ttgaaggatt ctacagggca aatgaggctg gcaaccaagg atttggcgga agccattaaa
    23581 cgaggagaag tacgtagttc tgcttttaca acaaagcaac taaaggcaat tgaaaaaggc
    23641 aaagacaaaa tccctagcta cacttggcat catcatcaag acactggaag gatgcaattg
    23701 attaatgaag acttgcatca tgataccggc catatcggtt ggagagcgat gagtaaagga
    23761 aagtaactat gtggaaaatc ataaaagagg atagtgatga tttaggattt gcaattaaat
    23821 gcttattctc tcagtctatt gatttaaatg aattcaagtt atggattgaa caagtaatac
    23881 gcgatatgcc catcgaggac atcccttttt atatttttga cttggcggat tttgatgggg
    23941 gaattgccga tattgacaat attgtaggtt ttgtttcgag ttacagccta tcaaaatcga
    24001 aaaaaaatgc cttgacaggc attgccttct taagggggat agatgtctat gatccgccta
    24061 tttcaaaaga aaaagcatta aaagccttaa agaaacatcc tgaaatttat cagaagtttc
    24121 agcatttctt tccgtttgta gaactgcccc cgctttaaac agtcaaaatg ccgtctgaaa
    24181 tgatattcgg ctttcagacg gcatttttga cacaaagcag gtaaccaaaa gagcgtttga
    24241 cggcaaagga agataattat gtgaaaaatt acgaaagagc aaggtcaaga tttaagtttt
    24301 gcaacatatt gcttattatc ggcagcaatt aatttgcaag aatttaaatt atggttggag
    24361 aaggttgtct tagatatgcc tattgataat ataccttttt atatttttga tttaattgat
    24421 cttaaagaag gagtaggaga tatttataat attttggatt ttgttccgaa ttcagactta
    24481 tctaaagacc aagatgacgc attaacaggc attgccttct taagggggat agatgtctat
    24541 gaccctcccg tttcaaaaga aaaagcatta aaagccttaa agaaacatcc tgaaatttat
    24601 cagaggtttc agcatttctt tccgtttgta gaactgcccc cgctttaaac agtcaaaata
    24661 ccgtctgaaa gccatttccg ccgctcagac ggcattttcg ccccttt
//
LOCUS       DAWFAN010000008        24083 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWFAN010000008 DAWFAN010000000
VERSION     DAWFAN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24083
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.008"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(431..2272)
                     /locus_tag="WGO65_000245"
     CDS             complement(431..2272)
                     /locus_tag="WGO65_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ0326603.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGVLPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFVLGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANMP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            2363..2833
                     /locus_tag="WGO65_000246"
     CDS             2363..2833
                     /locus_tag="WGO65_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ0326604.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAENQARLNPHEIA"
     gene            complement(2905..3717)
                     /gene="hpnC"
                     /locus_tag="WGO65_000247"
     CDS             complement(2905..3717)
                     /gene="hpnC"
                     /locus_tag="WGO65_000247"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ0326605.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(3804..4286)
                     /locus_tag="WGO65_000248"
     CDS             complement(3804..4286)
                     /locus_tag="WGO65_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0326606.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDSELVRIEDVGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            4759..5025
                     /locus_tag="WGO65_000249"
     CDS             4759..5025
                     /locus_tag="WGO65_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326607.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAPAA
                     EAPATEAPAAEAPAAEAAATEAPAAEAAASEAPAAEAPAAEAAK"
     gene            5135..6502
                     /locus_tag="WGO65_000250"
     CDS             5135..6502
                     /locus_tag="WGO65_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0326608.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYKHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELVGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(6683..8711)
                     /locus_tag="WGO65_000251"
                     /pseudo
     CDS             complement(6683..8711)
                     /locus_tag="WGO65_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9084..9530)
                     /gene="smpB"
                     /locus_tag="WGO65_000252"
     CDS             complement(9084..9530)
                     /gene="smpB"
                     /locus_tag="WGO65_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ0326609.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(9585..10595)
                     /gene="waaF"
                     /locus_tag="WGO65_000253"
     CDS             complement(9585..10595)
                     /gene="waaF"
                     /locus_tag="WGO65_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ0326610.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGCSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(10726..10801)
                     /locus_tag="WGO65_000254"
     tRNA            complement(10726..10801)
                     /locus_tag="WGO65_000254"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10766..10768),aa:Asn,seq:gtt)
     gene            complement(10992..11744)
                     /locus_tag="WGO65_000255"
     CDS             complement(10992..11744)
                     /locus_tag="WGO65_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ0326611.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVLIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            12055..12579
                     /locus_tag="WGO65_000256"
     CDS             12055..12579
                     /locus_tag="WGO65_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ0326612.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSATSA
                     ALCGTSSSVSTAAA"
     gene            complement(12668..12947)
                     /locus_tag="WGO65_000257"
                     /pseudo
     CDS             complement(12668..12947)
                     /locus_tag="WGO65_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(13318..14463)
                     /gene="dapE"
                     /locus_tag="WGO65_000258"
     CDS             complement(13318..14463)
                     /gene="dapE"
                     /locus_tag="WGO65_000258"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ0326613.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTGIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGRQGHIAYPHLAVNPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTNVARAAIVETCGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLEDIPKLSAVYEGILARLLAGNAV"
     gene            complement(14563..15183)
                     /locus_tag="WGO65_000259"
     CDS             complement(14563..15183)
                     /locus_tag="WGO65_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenosine tetraphosphatase"
                     /protein_id="HEZ0326614.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTDKYAAFDMWHGAESEYDDYRTVAQQNESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVVKWIAETVEDLLPLYEACHGA"
     gene            complement(15217..15726)
                     /locus_tag="WGO65_000260"
     CDS             complement(15217..15726)
                     /locus_tag="WGO65_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0326615.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHVSLYGN
                     WDAVAAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGKTFIPKEKLTEIG
                     EIMAARRRR"
     gene            complement(15819..16385)
                     /gene="azu"
                     /locus_tag="WGO65_000261"
     CDS             complement(15819..16385)
                     /gene="azu"
                     /locus_tag="WGO65_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ0326616.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPK
                     ASMGHNLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPA
                     KLADGDYKFACTFPGHGALMNGKITLVD"
     gene            complement(16666..17088)
                     /locus_tag="WGO65_000262"
     CDS             complement(16666..17088)
                     /locus_tag="WGO65_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ0326617.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            17219..19969
                     /gene="secA"
                     /locus_tag="WGO65_000263"
     CDS             17219..19969
                     /gene="secA"
                     /locus_tag="WGO65_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ0326618.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            19984..20136
                     /locus_tag="WGO65_000264"
     CDS             19984..20136
                     /locus_tag="WGO65_000264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326619.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            20114..21886
                     /locus_tag="WGO65_000265"
     CDS             20114..21886
                     /locus_tag="WGO65_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0326620.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            22073..24001
                     /gene="rpoD"
                     /locus_tag="WGO65_000266"
     CDS             22073..24001
                     /gene="rpoD"
                     /locus_tag="WGO65_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ0326621.1"
                     /translation="MSRNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         6219 a         6721 c         5597 g         5546 t
ORIGIN      
        1 aatcggttcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
       61 atccactata tccgactttc catacttaaa cagcactgcc tcttttcaga cattgacggt
      121 tgcagccgcc cacctgaacc ttaatgattg actatttttt aatcatgtta ttattttcca
      181 taaaatacat gacattaaga tgtttttcca caaaagatac acacaccggc aaacaccggc
      241 tgtgtttatc ttttcttatg cctatttttt aatcatcgta tttttatctt ttaatttcaa
      301 tacgcaaact aacttataca cacggttttc acatctttgg actgcttccg tgtgtatagt
      361 ggatattgcc gttttccttt ctgacaaaaa tgccgtctga gaacttcaga cggcatttga
      421 aacatcggaa tcagcggttt tgttcatacc actcgataaa cttgtctgct ttgacaaaac
      481 ccagcagcgg ctcgctgcgg ctgccgtcgg agcggacgac aaacacgccc ggcggcccga
      541 acagaccgta ttctttcaac aacgcctgat gttcgggcat gttggcggtt acatcgattt
      601 ggaaaaagcg ttccatatcg actgcctgat gcacttccgg ctgattgagc gtgtaagccg
      661 ccatttcttt gcaggaaatg caccagtcgg cataaaaatc caaaacgacg ggtttgtcgg
      721 gatgttcttt caacgccgta tccatcgctg ccttcagcgc ggcagtatcg gcaaacattt
      781 ttccgtgttc cgaagatttg cctgcttcag ccggcggatt gagggtcagg aaatggtgca
      841 gcgcggtcgt tttgccgttt gcgccctgcc agccgaacca cgcgccgcct atcagcaata
      901 tgccgcccaa tacgaatgcc acagctttcg gacggcgttt ctgcctgcgt ccgttgacca
      961 gcagcataca ggcaggaacc agcatcagca gcgtgtacag cgcgacgacg agataatagg
     1021 gcaagtgcgg cgtggcgagg taaacggcga cggcaagcag gataaagccg aaagcgtatt
     1081 tgacggcatt catccaatcg cctgccttag gcaggatatg cccgccgaac gtgccgatgg
     1141 caatcagcgg aacgccggtg cccaacgcca aagtgtaaag tgccaaaccg cctaaaaccg
     1201 catcgcccgt ctgaccgatg tagcccaaag caaatgccag cggcggggcg acgcacggcc
     1261 cgacaatcag cgcggacaat atgcccataa taaagacgga aacgatttta ccgcctgaaa
     1321 gcctgctgct ttgattctga aaatacgact gcacggcgtt gggaagctgg atgttgaaca
     1381 gcccgaacat agacagtgcc aagacgacca ttaaagccga tgccgccaac accacccaag
     1441 cctgctgcaa ccatacggtc agcagtgcgc ccgtcagtcc ggcaacaatg ccgaccagcg
     1501 tataagtcag agccaaaccc tgaacataaa cgacggacag cacaaacgcc cgcgccttgc
     1561 ccgccttttt gtcgccgacc acaatactgg aaacaatcgg caacagggga tacatacagg
     1621 cggtaaaact cagacccaaa ccggcgagaa aaaacgccaa aagattggcg ttgagcgtat
     1681 cccaagacag cttgaaacgg ctgtcgccgc cctcatcccc cttcgggggc ggcagcaccc
     1741 cgctgccgtt ttgagaggaa ggctgcaaaa agcggtcttt ggcggatgcc ggttcgtcgg
     1801 tttgcggatg gtaagtgccg ttgccggaaa tatcaaactc ggtatccacg ggcggatagc
     1861 acacgccggc ttcggcacag ccctgatagg tcaaaaccaa tttatacggt tcgccgacag
     1921 cctttgcata aggaaaggca acctgcgctt cgtgatggta aaccgtctgc ctgccgaaaa
     1981 actcgtcttc cttctcttca cccttgctga aagaaggctg tcccaacaaa tccgccggat
     2041 cggtcttgcc gacgattttc gcctgataca tatagtatcc gtcggcaatc ctgaaacgga
     2101 cgttcacacc gtcgtcggca acggcaagct ccggcacgaa tgccttttcc ggcggcagca
     2161 gatcgttcgc atccagcgcg aaagctcgtc cgcacaacat caaaaatacg gcgaacaggc
     2221 aaatcagttt tttcataatc gaatccgttt cagacaaata atttgtctgc attataaatg
     2281 gtaaggttga cggtgggatt taatttatgt aaaacccgcc attatccgaa cctatttcca
     2341 taaacatctt atcgaacccg ccatgtacga tgtcaatacc cacgatgtcc gccgcttttt
     2401 cgcccgcgtg tggcagcagc ggctcaatcc gctgcaactg agcgcactgg aacagaaagc
     2461 cctccgcatt atcgaagccc atcccgaata ccaccgttat ctcgaacgca tcgaagacca
     2521 tctggacacc gactggctac ccgaaaacgg cgaaagcaac cccttcctgc atatgtcgct
     2581 gcatttgtcc gtccaagaac aggcgggcat agaccagccg cacggcatac gcgcaatcca
     2641 cgacaccctg tgcgccaaac gcggctggct ggaagccgaa cacgaaatga tggaggcact
     2701 ggcggaaaca ctgtggacgg cgcaacgcta cggcaccggt ttggatgtca atttctacat
     2761 gacccgactg cgcaaactca tcggcttggg tgcagaaaat caagccaggt tgaacccgca
     2821 tgaaatcgcc tgaccatacc aaccgcctgc aaaatgccat ctgaagcgga acaacccctt
     2881 tcagacggca tgcatttccc ccaatcattt ccacaacgcc tttttcagca taatcaacca
     2941 atccttctta tccaaaacgg ggcgttgtgc aaacacatcg tatcggcacg cgtccagttt
     3001 ctgcaaaatc aactgcgccc ccaacacaat catacggagt tccaaaccga tacgcccctt
     3061 cagttccctt gccaaaggcg aacccgcctt cagcatacgg aatgcgcgtc ggcactcata
     3121 cgccatcagc cgctgaaacg ccgcatccgc ccgtcctgcc gcgatctgtt cctcagaaac
     3181 accgaatttc aacaaatcgt cctgcgggat ataaaccctg cccttttgcc aatccacagc
     3241 cacatcctgc cagaaattga ccagttgcaa agccgtacaa atgccgtcgc tttgcgccac
     3301 gcacaccgca tccgttttcc cgtacaaagc cagcataatg cgtccgacag ggttggcgga
     3361 acgccgacaa taatcggcca gctcgccgaa atgcgcgtac cgcgttttga ccacatcctg
     3421 agaaaatgca gaaagcaaat cataaaacgg ctgcaaatcc aaaccgaacg gcacaaccgc
     3481 ctcggcatcc aatcgtgcaa tcaaaggatg cgccgaccgg ccgcccgatg ccaacacgtc
     3541 caactcgcgc cgcaaaccct ccaaccccgc caacctagct tcagacggca tactgccctc
     3601 gtccgccata tcgtccgccg tccgtgcaaa cgcatacacc gcatgaaccg gcttcctcaa
     3661 cctgcgcggc aaaatcagcg aaccgacggg aaaattttca taatgcccaa ccgacatacc
     3721 ttctccatcc atcaaacaaa atgccgtctg aaacggaaca aacccttttc agacggcatc
     3781 agatacctcc aagctgccgg caatcagtgg tggtgatgac cgtgcgggcc gtggacatga
     3841 ccgtgtgcga tttcctcatc ggatgcatcg cgcacgcttt caaccgtagc cttaaaacgg
     3901 attttcatgc ctgccaaagg atggttgccg tccaccaccg ccttgccgtc ggcaacatcg
     3961 gttacacgat agacgacaac atcgccggtt tcaggatcgt cggcttcaaa catcatgccg
     4021 acttcgactt caacagggaa cacgcccaca tcttcaatac gaaccaactc cgaatcctgc
     4081 tcgccgaacg catcgtcggg cgaaagcgcc acatcgaccg tatcgccggc atccttaccg
     4141 tgcaacgcct cttccaccaa agggaaaatg ccgtcgtaac cgccgtgcag atacgcaatc
     4201 ggttcttcgg ttttgtccaa aagctgattg ttggcatcat acatctcata atgcagcgaa
     4261 accacggaat ttttcacgat agccatattt gtcctttcag gaacagcaga ttgattacag
     4321 gcgcattcta acacaaccgc cgcgccggcc gattaccgtt aacctgttca taaactgtac
     4381 agcacatatt tcaatgtaaa tttttgttat tttattgcgg tgtaactttt ttacaacatt
     4441 cttaaaacca ttccgatctg tctgccgact ttcccaatcc gccttaataa atcatacaag
     4501 atactgaaat tatattaatc tctataatat ttatccctat cgaattttta acagcaaaac
     4561 cgttttacag gatttatcaa tccgcccgcc agaaaacttt tcattcaaac ctttttccca
     4621 tctgtacgac attgcaatcc cttattccat agtgcataat tacgcaaatt cagcgatgaa
     4681 tttccaaccc ggtttgtagt atggtcgata aagacctatt tgtttcaata atttaaattg
     4741 gttctaaagg ttactaaaat gaaaaaatcc ctgtttgccg ctgctttgtt gtctttggtc
     4801 ctggcagcct gcggcggtga aaaagccgct gaagctcccg ctactgaagc acctgccgcc
     4861 gaagctcccg ctgctgaagc acctgccgcc gaagctcctg ctactgaagc acctgccgcc
     4921 gaagctcccg ctgctgaagc tgccgctacc gaagcacctg ccgctgaagc tgccgcttct
     4981 gaggctcctg ccgctgaagc tcctgctgcc gaagctgcaa aataagcatt ttccgcttgc
     5041 aaaaaagcag gatacgttca gtatcctgct tttttgattt ttcagacggc atcagattcc
     5101 cttcctcaat cttctcccta cccttccgac aaacatgcct gaccttcata ccgaattttc
     5161 ccgactcctg ccggcagatg aaattgccga actttctccg acgcttttaa aagaccagcg
     5221 caaccgcttt acgtctgcac cagacatcat tttgcagccg cgcagcgttg aaagcgtgca
     5281 aaccattatg cgtttttgtt acaaacaccg tattccggtt acgccgcaag gcggcaatac
     5341 cggtttgtgc ggcgcggcag tatcggaaaa cggcgtattg ctgaaccttt ccaaactcaa
     5401 ccgcatccgc agcatcaatt tgtcagacaa ctgcataacc gtcgaagcag gttccgtact
     5461 ccaaaccgtc caacaggcag ccgaagcctc aaacaggctg ttcccactca gtctcgccag
     5521 cgaaggctcg tgccaaatcg gcggcaacat cgcctgcaat gccggaggtt tgaacgtatt
     5581 gcgttacggc acgatgcgcg acctggttat cggtttggaa gtcgtcctcc ccaacggcga
     5641 actggtttcc catctccatc ccctgcataa aaacaccacc ggctacgacc tgcgccatct
     5701 gtttatcggc agcgaaggca cactgggcat catcactgcc gccacactca agctttttgc
     5761 ccgtccctcg gacaaggcaa cggcatgggt cggcataccc gacatcgaat ccgccgtccg
     5821 cctgctgacc gaaacccaag ctcactttgc cgaacgccta tgcagttttg aactggtcgg
     5881 ccgttttgcc gccgagttgt cttccgaatt cagcaaattc cccctgccga cacattcaga
     5941 atggcatatt ttacttgagt tgaccgactc attacccgac agcaatcttg atgatcggct
     6001 tgtcgaattt ctttataaaa aaggatttac cgacagcgta ttggcacaaa gcgaacaaga
     6061 acgtatccat atgtgggcat tgcgcgaaaa catctccgca tcgcaacgca aactgggcac
     6121 cagcatcaaa cacgatattg ccattcctat cggacgcgtt gccgactttg tccgccggtg
     6181 cgccaaagat ttggaacaga attttaaagg catacaaatc gtctgcttcg gacatcttgg
     6241 cgacggcagc ctgcactaca atactttcct gcccgaaatc ctcagcaatg aagtctatcg
     6301 ttacgaaaac gacatcaacg acgcagttta tcgcaacgtt cttgcctgca acggcacgat
     6361 tgccgccgaa cacggcatag gtatcatcaa aaaacagtgg ctggacaaag tacgcacgcc
     6421 tgccgaaatc gccctgatga aaagcatcaa acaacacctt gatccatata acattatgaa
     6481 tccgggcaaa ctgcttccgt aaccggcatt tctgatttgc atacacaaca aagaaaggga
     6541 caatagatcc gattgtcggt ttagcgcgag ctcgtgagtg cggtaaaaaa ttggtggaaa
     6601 tgacacgaaa aatgaccgca tttttaaaat aaaaaaatcg gcagtaaatt tctctgccga
     6661 ttttattttg ttacaactta acttaaaacg tccactgtaa attcaacgca ccttgtttag
     6721 cctgatgatg tttgcctgtt tggcggttga atgttgcttg taaagttaag tgagatttga
     6781 ttttcactgc tacacctaat tggctctcaa ttgccgtctt attgtttatc actcgacgct
     6841 ctccgtctat ttccacaccg aaaggtttgt tgtggtaaag cgcgttcaca gcggcgaaag
     6901 gttcaatagc gatattttta tagagcgaaa attgcgcttt agcttgaacg ccaacccgag
     6961 tttgtaattg gcgggagcca agtaaattca cgtgggcatt ttcgctatcg ctgaattttc
     7021 cgtttacccc caaataagtc aattgtgcct gtggttgtag gtaaacacga aggctgttgc
     7081 cctttttagt gaagtgttcc gccaataaag cattgtaacc cgcttcaatt gaggcggtaa
     7141 taccttttga agtaaaacgt tctgtagcat cttcagtgtt aatgcggtgg cggaagcgtt
     7201 gatattgtac ccagctatcc gcatacgcac ctgtctgttt gtcctgaagc tgatgccaag
     7261 tggcgtaaac gcctgcacca aagcctttca catttcccgt tgtaagattg tctgtatctg
     7321 ggttgcggaa agtactgcgt tgttctgcct gtccgcccat taagccaata gaaagttgat
     7381 tactttcgtt ttgccatgtg aatacttcgc cgccgagttg cacacctttt cgatttccct
     7441 ctactggcgt ggttttacct tgcacccatt gcttagaatg tccgtcaatc acacgcaacc
     7501 acaagccttt gcgtggtaaa gtgcggtcaa aaatatcgct gtttttgtcg ttcaaacgca
     7561 aggcgaataa ggtattggcg gcttgagcct gttgtgcata aatcgaaata tcatcgcgtt
     7621 cttgcacttt ggtaaaaaag ccttctgggc gttgttgtaa agaaagcgta taaattccct
     7681 tttggtgttt gccagaaaga cggaatgcgt gtttatctgc tgtgccattt actttgataa
     7741 tttgatgccc gtcgagactt tttaaatcgt ctattggatt ttcgaagatg atgtcggaag
     7801 tgccagttat atttttttca aaagttaaaa ctggtgtttc agggacttta tcttttagac
     7861 cgtaagcaaa acgaaaacga gctccgccag cataatcttc ttttacgagt aaactttcac
     7921 ttttagtatt aaaacggatg tctgcattcg ttgtttttaa ttccccaaca tcagaatccc
     7981 aacggggctc ccagagagaa ttttctaagc ggaattcatc caaactaatc gtttgcccga
     8041 taacgtgcga gttgtctgta acctcaatat agtggaatgg atctacatca gaatagagat
     8101 gctcttgcga actagaaagc tgattaagtg ttgggcgaag ccattcgata taattaccta
     8161 cagataaaat ttcgctgttg atatggctat tttttatttg tggtgctaag gcgaaaatat
     8221 cgctctttat gcgtatcggg gcatagcttc ctaaggatgg ttgtctacac ttatcattat
     8281 tggaattccc agtatttaca cagtccttaa aatattgccg gagtggtttt tcttcttccc
     8341 catttctttt ttcaaccccg aaacctcggg cgaagccaac taggtaatct tcggtatatt
     8401 tttgatcata aaaagaaatc ttttttgagt tgttgatgtt ttcgaattgg tgtgttttag
     8461 agaatagtgc gggaactttg agagagttct gagagctacc attcgtcggt tttagaagat
     8521 aagtttcttg tttccagtat tcgctgcttt tatctctaag tttttttaag cgatcaattt
     8581 gctcatttgt taatttagaa ttgtcgtaaa cgtaatcaac agccataaag aggagaggta
     8641 taaagaatag aaaaaaatag acttacaata aatgattttt taaacttctg cttgcttgct
     8701 tcgagtttca taataaattt tcctttgtca agtaaagata aatggggcgt ggattttagc
     8761 atagaattga acaaaaaatg tcatttagct cacatttttc tctatttatt tcttatttat
     8821 taaaagtaaa cgtttgcttt ttgctatttt gtcaagccag tttgaaaatg tgtataattg
     8881 ccctcgttat tcacaaaaat ttcaggaaaa atgaccgcac tttacccttg gttaatgcca
     8941 atttatcatc aaattgctca aacctttgac gaaggtttgg ggcatcatgc cgtgctgatt
     9001 aaagcggatt ctggtttagg tgtagaacgt ttacacatca ggcggcagcc ttgcccatac
     9061 cgtctgaagc actgtttcca caatcagcgc gtatgcttaa tcaaccgctg tttctcgcgt
     9121 ttccaatccg cctctttcat actctgacgt ttgtcgtgct gtttcttacc ttttgccaaa
     9181 ccgatttcca tcttgatttt tccgcgcgaa aaatgcaaat ccagcggcac aatggtgtag
     9241 ccggcacgtt cggttttgcc gattaatttg ttgatttccg actggttcaa caagagcttg
     9301 cgcggacgta cggcatctgg tttaatgtgt gtcgaggctg tgggcaaagc cgtaatatgg
     9361 cagccgacca gataaaacgc gtcttttttc caatagatat aactctcttt aagctgtacg
     9421 cgcgcggcgc ggattgcttt gacttcccag ccttccaaga ccaaaccggc ttcaatccgg
     9481 tcttcaatga aaaaatcgtg aaatgctttt ttattgttcg caatagccat aaacatccta
     9541 tcaatatccg ccgtcagacg gcataaaccc gaaaacagaa cccatcatac cgcctcttca
     9601 accgcctgca caatcttctc gggatacagc ctgttgaggc agtcggtatg ccccaacgga
     9661 cattcacgtt tgaaacaggg cgaacattcc aagtgtaggc tgacgatttt cgccctgtcg
     9721 ctcaaaggcg gcgtatgcgt cgggctggaa gaaccgtaaa ccgccaccac tttcctgccc
     9781 aatgccgccg ccaagtgcat cagcccgctg tcgttgcaca cgaccgtgtc cgccaacgac
     9841 agcaaatcca ttgcctgcga caaatcggtt ttgccgcaca aattgacaca cataccgtct
     9901 gaaaggcggt tgatttcctc ggcaatttca tcatcttttt gcgaaccgaa caaccaaacc
     9961 tgccaacccg ccgccagata atgtttgccc aactcggcaa aatgccttgt cggccaacgc
    10021 tttgccggcc cgaattccgc acccggacaa aaagccagaa caggctttcc aagattcaaa
    10081 ccgaaagttt cgacagaaat ttcccgccgc cgttcatcaa tggaaaactc gggaaatcct
    10141 gaacacccgt caaaatcttc ctgactctga tgcgcgagag ccgtataccg gtccaccatc
    10201 aaaggcagac gttccttatc cagcctgcgt atatcgttca acagaaaata acggctttca
    10261 ccgacataac ccgtcctttt accgatacct gtcgccagcg cgatgattgc cgatttcaaa
    10321 gaaccgggca acacgataac ctgatcgtat ccgcgccgcc ccaaatccct accgacccgc
    10381 caacggcgtt tcaactccaa cgcaccgtgt ccgaacggat tttcaagaat ctcattcact
    10441 tccggcatac gctcaaacac cgccatcgac cacttcggtg cgaacacatc aatcgtgcaa
    10501 ccggggtgaa gttccttcaa acggcggaac aagggctggg tcatcacgca gtcgcctatc
    10561 caactgggag aaataatcag gattttgatg gacataacaa gaaaccgaaa tcagacaggc
    10621 agaattttac cgcgaaaccg ttggaaaacc tatcttgccg cattccgaac gccgtacgtg
    10681 caaatatgaa aaagcccgaa cattcaagtt cgggcttcaa aattctggct ccccgacctg
    10741 ggctcgaacc agggacctgc ggattaacag tccgtcgctc taccgactga gctatcgggg
    10801 aatggggcgt attatagcgt ccggaaaaaa tgtgtcaatc cttaattttg gaaaaatggg
    10861 cgacaaaacg acaagcatat gaatcagaaa gacattaaga ccgatgcctt aaaaggattg
    10921 ccgttgtatg aatttccaca gccgtcatca caccatattt aagcccgatg agccgttctg
    10981 ccctcccccg cttaaaacaa tgccgtctga acttcgccgc gttccaaagc cagtaaaaac
    11041 tgtttgcggt tcaacccgcc cgcgtagccg gtcagtttgc cgtcgctgcc gatgacgcgg
    11101 tggcagggaa tcaggacaga caccttgttc tgcccgttgg cggcggcaac ggcgcggacg
    11161 gctttggggt tgcccaaacg ctgcgcctgc tccttgtagc tgcgcgtttc gccgtaagga
    11221 atcgccaaga gcgcgtccca tgcctgcttt tgaaactcgg tgccaatctg ctccaaaggc
    11281 gtggcaaagg ttttcagacg acccttgaag tataagtcca attcctgccg caaaagttgc
    11341 gtccgctcat cctcccgaaa cacaaaccgt ccgcgcaagg ctttttggac ggcggcaatt
    11401 tcctgttcca aatgcttctg tccgacaaac tccagcagac acaatccctt atttccgaac
    11461 accgccagca tctcgcccaa aggcgtggca atggcggcac acaccagctc attcaaactg
    11521 tcgggataac gcgcttccaa cagacggatg gctcggcgga tacggacata ttcttcaggc
    11581 gcgcagccga tattgtccca aaaatcccga tcgaactgcc cggcttcgca ttccgtcaaa
    11641 ttgggatgcg gcataacgcc gcactcacaa acccgggttt cgagccaata gcggatttca
    11701 tcccattttg acggcagatt gtttaaggaa ggcagggtaa tcattcgttt gctccgtatc
    11761 tctatcatgg atttgacggc aaaattccca attttcgcca ttcccgcacg ctgatgcagg
    11821 aacgggctac gacgtaaatc ttgagggtta ggttgcggca atgcctaaat atgcgatatt
    11881 tccaaagcct cagggaaagg aattaaagac ttcagacgac ctgcaaaggc tcgtctgaaa
    11941 ccacgatttt tgcatttgcg cattctggca catcatccaa ccgtttcggc acattcctgc
    12001 cgccgttgac agcctataat gaatccactt attcatcaag caaaggaatc atctatgcaa
    12061 acccgcattc tctccgccgt actgctggct ttttcaaccg ctgcctttgc cgggggcgca
    12121 ttcacgctgc aattcgacaa cccgtccgaa gacggcggct tcacgcaaaa ccagcttttg
    12181 agcgcgcctt acggatttgg ctgttcgggc ggcaatgctt cgcccgcgct gtcgtggaaa
    12241 aatccgcccg ccgggacaaa aagtttcgtc ctgaccgttt acgataaaga cgcgccgacc
    12301 ggactgggct ggatgcaccg ggtggtcgcc gacattcccg ccgatgtcca ccgccgcaac
    12361 gcgacctcgc tgcaattaag ccgctgcgcc aacatcgccg acgaccagtc cgcagccata
    12421 tcggcggtaa tcagtttgcg gatttgccgc atcaggttga cgccttcgta cacggcaaaa
    12481 ccgatgccgt catgctgcaa ccacgccaac acgccgcaaa gcgcgacctc cgccgcattg
    12541 tgcggcactt cttcatccgt cagcaccgcc gccgcataat caaacgccgc gcccatccgc
    12601 ccggggtgcg gcagcttcac cgcatcgcac actgcctgcg ccgtcccgta ttgtgcggca
    12661 aacctttcta tggcttcctg ttcgaaagca atccattgcg cctgatagag gccgtctgaa
    12721 tcgggaatat taataacgtc aaacgtctgc ccgcctgcca aggcgaccgc ctcacccgcc
    12781 gcaatcctca attcgcgcct cgcccaccgc agcttcttgc ttccgcgccg cacgataagc
    12841 acagccggtt catataccgc cacgctgcgg tacaaggcgg tatgatgttg cacgatgccg
    12901 cccaaagcac ccaatcgttc gcgcgtatga aagtaatttg acggcactat tatctccaac
    12961 acgcagggcc gcacataagg cgcagccctg tttaccctga tttgggaggg ttacttccca
    13021 aaaataaacc aaaatcccac cgccccgaat agacgggttt caaccatatt ccgtcggcgt
    13081 acctgcaact tggctccctc ccctgtgagg gagggtcggg gagagggtaa aacggggcag
    13141 atacaggcaa tatttccgtt gccgccccaa tgccctctcc ctaaccctct ccctcgggag
    13201 agggaatgga ttgccgttga aataaatcgc tctacataaa aatcaatgcg ttatctcaaa
    13261 cccacgttag gtctaatcaa atggtcggac atccgtatcc gacagcccat cttcttttca
    13321 gacggcattt ccagccaaca accgcgccaa tatcccctca tacaccgctg acagcttcgg
    13381 aatatcttcc agccgcacgt tttcgttgat ttggtggatg gtggcattgg acgggcctaa
    13441 ttcgatgagt tctttcgcaa tggctttaat gaagcgtccg tccgaagtgc cgccggtggt
    13501 ggacagttcg gtctccacgc cgcaggtttc aacgatggct gcgcgtgcca cattggttag
    13561 tttgcccgca tgggtcagaa agggctgccc cgaacacgac cactgcaaat cgtattgcac
    13621 gccgtgtttg tccaaaatgg cgtggacgcg ttgtttcagc cctgcttcgg tggactcggt
    13681 ggagaagcgg aaattgaatt tgacgttcag ctcgcccgga atgacgttgg tcgcgcctgt
    13741 gccaccgttg atattggaaa tttggaaact ggtcggcgga aagtattcgt tgccttcgtc
    13801 ccatacttcc tgcgtcagct ctaacaaggc aggggcaaaa gtgtggacgg ggttcaccgc
    13861 caaatgagga taggcaatat gcccttgcct gcctttgacg gtcaggttgc ccgacagcga
    13921 gccgcgccgg ccgtttttaa tcatatcgcc caatttgtcc acggcggtcg gttcgccgac
    13981 gatgcagtag tcgataagct cgtcgcgcgc tttcaacaca tcgacgactt tggtcgtacc
    14041 gtccagcgcg tcgccctctt cgtcggaagt aatcaggagc gcaatgctgc cttgatggtc
    14101 gggatgttcg gcaacgaaac gttcgcaggc ggtaacaaaa caggcgatgc cggttttcat
    14161 gtccgccgcg ccgcgcccgt ataatcttcc gtcgcgctca gtcggttcga acgggggcga
    14221 atcccatttt tcaacagggc ccgtcggcac aacgtcggta tgccctgcaa aacagacgac
    14281 gggagctttc gtgccgcgtc gcaaccagat gtttttggtg tcgccgaaat ggagttcttc
    14341 agccgcaaaa ccgattttgt gcaggcgttc ggcaaggagt ttttggcaat ctcggtcgtc
    14401 gggggtaacg gatgggcggg aaatcaattc tttggcgagt tctagggatt gggtttcggt
    14461 catttttcgg cttttctgtt tgagatgccg tctgaacgga gtttcagacg gcatttgatg
    14521 ttgaatggaa cgggctaacg atagcgaagc gggttcttca ggtcaagcac catgacacgc
    14581 ttcgtaaagc ggcagcaaat cttccactgt ttccgctatc catttcacga catcctgctt
    14641 gcccaaatcg tcgcgctcga tgtgtttgcc gatgcagaag aaatcttcgt cgttttgcaa
    14701 ccttcggtcg gattcatttt gctgcgcgac ggtgcggtag tcgtcgtatt cgctttccgc
    14761 accgtgccac atatcgaatg cagcgtattt gtcggtatcg aaatcatcca gccagcggtt
    14821 gtattccggc agtgcaatcg gcgacacgtc ggctttgtag caatgccagt ccaagctgac
    14881 actcaaacgg cggcggttga gcagtatcga caaaatcgcg gcggaatttt tatattgttc
    14941 gtatttgaag taggcaaaga aatgggcgcg aacctgccag ccgttgcacc agcgttcgat
    15001 gtgcggcggc gcaaacggcg cacccaattc ggcggcaacc tgctgaatca gctgctgcca
    15061 tatctgccag ttttccttgt agtcggcttt gatttgcgga atgctttcag gctggtattt
    15121 tttaagctgg gaaaattgga aaaacgggat attgaacaaa tcgcaacttt tcggggtcag
    15181 cataatatat ccttgagacg attgtttcag acggcattat ctgcgccggc gcgccgccat
    15241 gatttcgccg atttcggtca gtttctcttt tgggataaag gttttgccca tgtcaaataa
    15301 gggttcttca atcgccaaat gaacatcata tcccgccaca aaacgtttga acgcttcctc
    15361 atcggggaca taagcgttgt ctgcttcgag tttggcaaat tcggcagcca cggcatccca
    15421 gttgccatac aggctcacat gctgacgcaa aagctcgtcc acgctttctt gggcttgcgg
    15481 cgcgtattgc agcagcagcg ggaagaagtt ttcttcttcg tcttcatggt gcagcggcgc
    15541 ggcaacgttg aaatactggg cgatttggcg gatggtttgc aaaacaatct gattgcagcc
    15601 gttttcggcg atatagtccg acagcatggc gacttgtccg cagaaacggc gcactttgcc
    15661 gtggcaggca tacagcattt caatcggttc ggcaaaggta acgcttttgg tttcaaacgg
    15721 attcatggtt tttaggtttt attcttaaat taacgtttcc agattataac aaaacagcct
    15781 gctcaaggca ggctgtctgt tttcaagctt taggcgaatt agtcaaccaa agtaatttta
    15841 ccgttcatca aagcaccgtg acccgggaag gtgcaggcaa atttgtagtc gccgtcagcc
    15901 aatttggcag gatccagagt cagggaagac tcttcgccgc cgccgatcag tttggtgtgg
    15961 gcaacaacgc gcgcatcgtc aggtttgaca tagtcggtat cggcagcacc tacgccgtct
    16021 ttaaatacgc cgtccatgtc ttcagctttg gcaatcacaa ggttgtgacc catgctggct
    16081 ttgggttgcg taccggtatg tttcagagtg atggtaaact ctttacatgc tttgctgact
    16141 tggatgtctt tggtgttgaa ctgcatattg tcgttggatt cgacagttgc cgcacagttg
    16201 ccggcagcag gggcttcggc agcatctgca ggagcagcat ctgcaggagc agcttcggcg
    16261 gcaggtgctt cggaagcggg tgcttcagca gcaggagttg cctcggcagc aggcgcggca
    16321 ggttcttgag agcaggcagc caaaccgata acggcagcag aaatcagagc cagatacgct
    16381 ttcataacaa atctccaatc gataaaataa tattcggttt tacaggaatc aaagtgcaac
    16441 cgccattaac aaaaccttga aaaagattcc gccgcgttgc acaaacagat gtttcggggt
    16501 ggcattttgc tacaaatttc atttaaaatc aaagcctgtt tgcaagttta caatcgttta
    16561 cccaaaaaag ggcaatttta ccccgaacct atttctttag tattagaccc attatccttt
    16621 acttcttaat attaacggat gtttacacaa attcccgtat acattttatg cgccatgcct
    16681 tctgaccaag tttgccaatg cctccgccaa ttcgggatgc cgtttttcca actttaccgc
    16741 cgccgaaccg aaactctcca gcgcagcctt actcaaatgc agggtattgg ttttcggcgg
    16801 tttttccggt ttcgggacca gcctgaccga aacagagcgt atcgaagcat caagccctgc
    16861 caactgcggc aataccgacg gtgcaatcat tttcaagcgc gatgccgcca tattgtttgc
    16921 cgccaaaagg acaagcctgc cgtcttcgat acatgccgtc tgaaaatgcg ggtgcaggtt
    16981 ggcaggcagc agttttttca cggcggcatc caaccgccgc cactgtcccg cctgtttcaa
    17041 aagtccggaa agcagcgcgt cccgcctgcc caactgttcc aaattcataa aacatacacc
    17101 caaaaagatt gaaataccgc aaacgcgcct ttatttcaga cggcattagc actttgcaca
    17161 aacgcttgtg ttaaaatcgc gttttcgccc actattatat caggcgcagg aattattcat
    17221 gctgacaaac attgccaaga aaatcttcgg cagccgcaac gaccgcttgc tgaaacaata
    17281 ccgtaaatcc gttgccagaa tcaacgcgct cgaagaacag atgcaagccc taagcgatgc
    17341 tgatctgcaa gccaaaactg ccgaattcaa acaacgcctc gccgacggtc agactttgga
    17401 cggcattttg cccgaagcct tcgccgtctg ccgcgaagcg tcccgccgca ccctcggtat
    17461 gcgccacttc gacgtgcagc ttatcggcgg tatggtgctg cacgacggca aaatcgccga
    17521 aatgcgtacc ggcgaaggca aaaccttggt cgccaccctc gccgtctatc tcaacgcgct
    17581 ggccggcaaa ggcgtacacg tcgttaccgt caacgactac ctcgcctcac gcgatgcggg
    17641 cattatggag ccgctctaca atttcctcgg ccttaccgtg ggcgtgatta tttcagatat
    17701 gcagccgttc gaccgtcaaa acgcctatgc cgccgatatc acctacggca ccaataatga
    17761 attcggcttc gactacctgc gcgacaatat ggttaccgac caatacgaca aagtgcagcg
    17821 cgaattgaat tttgccgttg tcgatgaagt ggattccatc ttgattgacg aagcgcgcac
    17881 tccgctgatt atctccggtc aggcggatga caacatccag ttgtaccaaa tcatgaacac
    17941 cgttccgccc cacctcgtcc gtcaagagac agaagaaggc gaaggcgact attgggtcga
    18001 cgaaaaggca catcaggtca tcctgagcga agcaggtcac gaacacgccg agcaaatcct
    18061 gacccaaatg ggattgctgg cagaaaacga ctccctctat tccgccgcca atatcgccct
    18121 gatgcaccac cttatggcgg cattgcgcgc gcattccctc ttccacaaag accaacatta
    18181 cgtcatccaa gacggcgaaa tcgtcatcgt ggacgaattc accggccggc tgatgtccgg
    18241 ccgccgctgg tcggagggtc tgcatcaagc cgtcgaagcc aaagaaggcg tggaaatcaa
    18301 acgcgaaaac caaacccttg cctccatcac cttccaaaac tatttccgcc tgtacaccaa
    18361 gctctccggc atgaccggca cagccgatac cgaagctttc gagttccaaa gcatctacaa
    18421 cctcgaaacc gtcatcatcc cgaccaaccg ccctgtacag cgcaaagact tcaacgacca
    18481 gattttccgt tccgccgaag aaaaattcga agccgtcgtc aaagacatcg aagaatgcca
    18541 caaacgcggg cagcccgtcc tcgtcggtac gaccagcatc gaaaactccg aactggtctc
    18601 ccaccttctg caaaaagccg gactgccaca caacgtcctc aacgccaaag aacacgaacg
    18661 cgaagcgctg attgtcgccc aggcaggcaa agtcggcgcg attaccgttg ccaccaatat
    18721 ggcgggacgc ggtacggaca tcgttttagg cggcaacctg aagcaccaaa ccgatgccat
    18781 ccgcgccgac gaaaccttga gcgacgaaga gaaacaggca caaatcgccg cactcgaaga
    18841 cggctggcag gcggaacacg acaaagtgat ggaagcaggc ggtttgcaca tcatcggtac
    18901 ggaacgccac gaaagccgcc gcatcgacaa ccaattgcgc ggacgttccg gccgtcaggg
    18961 cgaccccgga tccagccgct tctatctctc ctttgaagac ccattgctgc gcttattcgc
    19021 actcgaccgc gccgccgcca tcctcaaccg cctcgccccc gaacgcggcg tcgccatcga
    19081 acacaacctg ctgacgcgcc aaatcgaagg ggcgcaacgc aaagtcgaag gcagaaactt
    19141 cgatatgcgc aaacaggttt tggaatacga cgacgttgcc aacgagcaac gtaaagtcat
    19201 ttacagccaa cgcaacgaaa ttttaaccag caaagacatc agcgacctga tgaaggaaat
    19261 ccgttctgat gtcgtcagcg acctcgtgga tacctatatg ccgcccgaca gcatggaaga
    19321 acaatgggac atcccgactt tggagaaccg tctggctgcc gaattcagac tgcacgaaga
    19381 catccaatcc tggctgaagg cggacaatgc gattgacggt caagacatca aagaacgcct
    19441 gatcgaacgc atcgaaaacg aatatgccgc caaaaccgaa ctggtcggca agcaggcaat
    19501 ggccgatttc gagcgcaacg tgatgttgca ggtcatcgac aaccaatggc gcgaacacct
    19561 cgccgctatg gactacctgc gacaaggcat acacctgcgc agctatgccc aaaaaaatcc
    19621 gaagcaggaa tataaacgtg aagcctttac catgttccaa gacctgtgga acggcatcaa
    19681 attccatatt gcctccctgc ttacctcggt tcaaatcgaa caaaaccctg tcgcggtggt
    19741 tgaagagcaa cccatcggca acatccagtc catccattcc gaatcgcccg atatggaaga
    19801 acttttgggt cagtcgcaaa ccgatctggt taccgaagcc tttaatcccg atgggacaga
    19861 tttcagcccc gaagccttgg aagcgcgggg gcaaatcgtc caccgcaacg acccctgccc
    19921 ctgcggcagc ggtttgaaat acaaacaatg ccacggcaaa ctggcttaag cgtttgaacg
    19981 caaatgccgt ctgaacatcc cgctcccgtt tcagacggca ttttgcctga accgccacat
    20041 ccgactgcca ttccgaaaaa tcccgatttc gtaccgtccg taccaaaaac agacatcccg
    20101 tccgccccac atcatgattc catccgactt cattgacgag cttttagcca aaaccgatat
    20161 tgtcgatatt atcgacgagc aggttccgct gaaaaaaggc ggggcgaact atatggcgtg
    20221 ttgcccgttc cacaaggaaa aaacgccgtc gttttcggtc agtccaacca agcagtttta
    20281 ccattgtttc agttgcgggg cgcatggttc ggcgattggt tttgtgatgg agcatcaggg
    20341 gctgtcgttt ccggaggctg tgcagtttct tgccgaccgt gtgggcatgg ttgtgcctaa
    20401 agtgcgtggg cagaacgata atcccgaagt ccgtgccgaa cgtaagaaaa aacagcagac
    20461 gctggaagaa acgacggcgg cggcggctga tttttacgcg caacagctaa aattcaatcc
    20521 ggcggcaaaa gcttatttgg acaagcgcgg cttgagtgca gaagttatcg cgcattatgg
    20581 tttgggctac gcgcccgacg gctggcagcc tttggcgcaa gtgttccaac cgtatccgaa
    20641 taccgcgttg gtggatacgg ggatggtgat tgacaatgag gggcggtatt acgaccgctt
    20701 ccgccatcgg attatgttcc ctatccgcaa tccgcgcggg caggtcatcg gtttcggcgg
    20761 cagggtgctg gacgactcca aacccaaata tctaaactct cccgatacac ctttgtttga
    20821 caaagggaaa aacctttacg gcttgtatga ggggcgtgcc gctgtcaagg aagcggagcg
    20881 gattctggtg gtcgaaggct atatggacgt ggtcgcgctg gcacagttcg gcgtgggcta
    20941 cggtgtggcg gccttgggca cggcaacaac agctgaacac gtcaaaatcc taatgcgtca
    21001 ggcagacagt atttatttct gtttcgacgg cgacagcgcg gggcgaaaag cggcttggcg
    21061 cgcgctggaa aacgcgttgc cgcagttgaa agacgacaaa tcgctgcatt ttttgttcct
    21121 gcccgaggaa cacgaccccg acagttacat ccgtgcctac ggcaaagcgc aatttgaaga
    21181 cgcgcttctg aatcaaagca agcctttgtc ggagtatttc tgggaacacc tttcagacgg
    21241 cattcatctc aatacgcagg aaggcaaggc ggaattggtg aaaaccagtt cgccgctttt
    21301 ggcgcagatt accgcgccgg cattggctta tttgttaaaa caacggctta gcgaactggt
    21361 cggcatcgac cccgacaacc tcgcgcaact gctaggacag gaagcgccga agcggcacgt
    21421 caaacaaaaa aactacaaac tgcctccgat ttccgtcaaa cagcccgtca tgctgacgct
    21481 ggtacagcgg caaatccgca gcctcttgat aaatccggat tgggctgcat atatagacct
    21541 gcccgattat ctggcgttgg acggtgattt cgcctgcctt gccaatcttg ccgaatcgat
    21601 taaaaaccat gccgccgtac ccgaaaccgc tcaggtttta gagtatatgc gcggctcgcc
    21661 ttacgaagaa acgataaccc gaatcttcca ttcaacgcac caatcggaag aaatgaacag
    21721 cagcagtgaa gaagattgcg agaatttcca aatcggcatg aaaaaactgc tcaatgagtt
    21781 aaaatacagc caaatcgaaa cattaaaaca aaaaagcctg caatccggct taaatgaaag
    21841 cgagaaaaaa cttttgctgt cgttgctgac cgcaaaacaa aattgaccgg cggattccgc
    21901 cacccgtaaa cccgttatgc cgtctgaaaa gcacttaccc cggctgcaac aacgacacct
    21961 gcagaacacc catcccaaaa agccttcaga cggcatcaga gtacccgctc tgccacgcct
    22021 tcaggtgcgt tcaaacgcaa accgtcggca ccttaccaac agaaagcaga caatgtccag
    22081 aaaccaaaat cacgaagaat accaagacga tgcccgcccc ctgacgattg aggaacaacg
    22141 cgctcgcctg cgccaactca tcattatggg caaagaacgc ggctacatca cctactccga
    22201 aatcaacgac gcactgcccg acgatatgtc cgatgccgat caaatcgaca acatcgtcag
    22261 tatgatttcc ggtttgggta tccaagttac cgaacacgcc cccgatgcgg aagacatatt
    22321 gttaagcgac aatgccgccg ttaccgacga tgatgccgtc gaagaagccg aggccgccct
    22381 ttccagcgca gattccgagt tcggcaggac gaccgacccc gtccgtatgt atatgcgcga
    22441 aatgggacag gtcgacctgc tgacccgcga agacgaaatc atcatcgcca aaaaaatcga
    22501 aaacgccctg aaaaatatgg ttcaggccat ctccgcctgc ccgggttcca ttgcggaaat
    22561 cttagaactc atcgaaaaaa tccgcaaaga cgaaatccgc gtcgacgaag tcgtagaagc
    22621 cattatcgac ccgaatgaag tattgctcaa cgaattgggc ttggggcact tggaaaccac
    22681 agcacccgag aaaccttcca acgacaattc ggacgaaaac gaagacgacg aagaatcgga
    22741 agaagatgcg gatgaaatct cggcagccaa tctcgccgaa ttgaaacaaa aagtcatcgg
    22801 ccactttgcc caaatcgaaa aagactacaa aaaaatgatc ggtcgtttgg aaaaacacca
    22861 cagccggcac aaagactatc tcgcctaccg cgacgcgatt gccaacaaac tgctggaagt
    22921 ccgtttcgcc acccggcaaa tcgacagcct cagcagcagc ctgcgcggaa aagtagaaaa
    22981 catccgcaaa ctcgaacgcg aaatccgcga catctgcctc gaccgcgtcc atatggagcg
    23041 cgactacttc atccaaaact tcctgcccga aatcaccaat ctggaatgga ttgaagaaga
    23101 aatcgccaaa ggcagggttt ggagcgacgc gctcgaccgc ttccgccacg ccatcctcga
    23161 aaaacaaacc gagttggcag atatggaaaa agaaacccgc atttccatcg aagagttgaa
    23221 agaaatcaac aaaaatatgg tgtcgagcga aaaagaaacc gcagccgcca aacaggaaat
    23281 gattcaggca aacttgcgcc tcgtcatttc cattgccaaa aaatacacca accgtggctt
    23341 gcagttcctt gatttgattc aggaaggtaa catcggcctg atgaaagcgg tcgacaagtt
    23401 cgaataccgc cggggctaca aattctccac ctacgcaacc tggtggatcc gccaggcaat
    23461 cacccgttcg attgccgatc aggcgcgtac catccgcatt ccggtacata tgattgaaac
    23521 catcaacaag atgaaccgca tctcgcgcca acaccttcaa gaaaccggcg aggaacccga
    23581 ttccgccaaa cttgccgaac tgatgcagat gcccgaagac aaaatccgca aaatcatgaa
    23641 aatcgccaaa gagccgattt cgatggaaac ccccatcggc gacgacgacg attcgcactt
    23701 gggcgacttc atcgaagatg ccaacaatgt tgcgccggcc gatgcggcaa tgtacaccag
    23761 cctgcacgaa gtaaccaaag aaatactcga aagcctgacc ccgcgtgagg caaaagtcct
    23821 gcgtatgcgt ttcggcatcg atatgaacac cgaccacacg ctggaagaag tcggcagaca
    23881 gtttgacgta acgcgcgaac gcatccgaca aatcgaggca aaagcactcc gcaagctgcg
    23941 gcatccgaca agaagcgacc gtttgagaag tttcttggac agcgaagaca gcaagctgta
    24001 aaccaaaaaa ccgcaggttt caaatacctg cggttttttc ttacacaata aacaacgctt
    24061 ccacatatcc cacactccta tcc
//
LOCUS       DAWFAN010000009        23639 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWFAN010000009 DAWFAN010000000
VERSION     DAWFAN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23639
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.009"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(67..906)
                     /locus_tag="WGO65_000267"
     CDS             complement(67..906)
                     /locus_tag="WGO65_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0326622.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1191..1266
                     /locus_tag="WGO65_000268"
     tRNA            1191..1266
                     /locus_tag="WGO65_000268"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1224..1226,aa:Arg,seq:ccg)
     gene            complement(1331..2524)
                     /locus_tag="WGO65_000269"
     CDS             complement(1331..2524)
                     /locus_tag="WGO65_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0326623.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAATPVPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFESAGFEVGTYPYYDPAAVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2530..3633)
                     /gene="trmA"
                     /locus_tag="WGO65_000270"
     CDS             complement(2530..3633)
                     /gene="trmA"
                     /locus_tag="WGO65_000270"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ0326624.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     TVERAALFDQFPFTHHIESGVLLKKKILGKSKR"
     gene            3776..4876
                     /gene="aroC"
                     /locus_tag="WGO65_000271"
     CDS             3776..4876
                     /gene="aroC"
                     /locus_tag="WGO65_000271"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ0326625.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRRETDQVEILSGVFKGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4967..5392
                     /locus_tag="WGO65_000272"
     CDS             4967..5392
                     /locus_tag="WGO65_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ0326626.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5466..7451)
                     /gene="parE"
                     /locus_tag="WGO65_000273"
     CDS             complement(5466..7451)
                     /gene="parE"
                     /locus_tag="WGO65_000273"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ0326627.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7518..8042)
                     /locus_tag="WGO65_000274"
     CDS             complement(7518..8042)
                     /locus_tag="WGO65_000274"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ0326628.1"
                     /translation="MLDREGYRPNVGIILINERNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIEFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            8207..9502
                     /gene="serS"
                     /locus_tag="WGO65_000275"
     CDS             8207..9502
                     /gene="serS"
                     /locus_tag="WGO65_000275"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ0326629.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYEFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9575..10573)
                     /locus_tag="WGO65_000276"
     CDS             complement(9575..10573)
                     /locus_tag="WGO65_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0326630.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGKYVDLDELYAQSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10698..11774)
                     /gene="prfA"
                     /locus_tag="WGO65_000277"
     CDS             complement(10698..11774)
                     /gene="prfA"
                     /locus_tag="WGO65_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ0326631.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDTDDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11864..12634)
                     /locus_tag="WGO65_000278"
     CDS             complement(11864..12634)
                     /locus_tag="WGO65_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0326632.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILWIAEIAAQIRGVGVADIAAITERNADSLLRP"
     gene            complement(12749..13738)
                     /locus_tag="WGO65_000279"
     CDS             complement(12749..13738)
                     /locus_tag="WGO65_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ0326633.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     VKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGVIAEWDETQGWYNLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGRLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13805..14488)
                     /locus_tag="WGO65_000280"
     CDS             complement(13805..14488)
                     /locus_tag="WGO65_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0326634.1"
                     /translation="MFAFLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14679..16013)
                     /gene="glmM"
                     /locus_tag="WGO65_000281"
     CDS             complement(14679..16013)
                     /gene="glmM"
                     /locus_tag="WGO65_000281"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ0326635.1"
                     /translation="MAKKYFGTDGVRSEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVIQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVVDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAKVEKELEGKGRVVLRASGTEPVVRVMVEARQADWAREGAERIASAIGSL"
     gene            complement(16143..17000)
                     /gene="folP"
                     /locus_tag="WGO65_000282"
     CDS             complement(16143..17000)
                     /gene="folP"
                     /locus_tag="WGO65_000282"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ0326636.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRSGADYVSPEEEWARVEPVLAEVAGWGVPISLDTRR
                     TVIMEKALALGGVDIINDVAALNDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKPLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(17140..18033)
                     /locus_tag="WGO65_000283"
     CDS             complement(17140..18033)
                     /locus_tag="WGO65_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ0326637.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVCIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18124..20238
                     /locus_tag="WGO65_000284"
     CDS             18124..20238
                     /locus_tag="WGO65_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ0326638.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20303..21781)
                     /gene="ubiD"
                     /locus_tag="WGO65_000285"
     CDS             complement(20303..21781)
                     /gene="ubiD"
                     /locus_tag="WGO65_000285"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ0326639.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVIGMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21801..22316)
                     /locus_tag="WGO65_000286"
     CDS             complement(21801..22316)
                     /locus_tag="WGO65_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ0326640.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLLIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNWLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22323..22853)
                     /locus_tag="WGO65_000287"
     CDS             complement(22323..22853)
                     /locus_tag="WGO65_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326641.1"
                     /translation="MVRQGEATLYWFLLIHYHYGREAGWFVWLAAAMCGLWLLRALTA
                     KLPQQRYAALFITLFFAAVLVFGRRDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLA
                     RLQQPDLPEKAVRYTRRVTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMG
                     MPFAGEWLYRKLVLKV"
     gene            complement(23056..23625)
                     /locus_tag="WGO65_000288"
                     /pseudo
     CDS             complement(23056..23625)
                     /locus_tag="WGO65_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
BASE COUNT         5395 a         6736 c         6153 g         5355 t
ORIGIN      
        1 attcccataa aaaaacggag cagatacctg ccccgttttt atttaatccg aaattttaat
       61 ctaaatttag aattttgcac cggattggtt tgccatatag tcaacagccg ctttgacttc
      121 gtcatcgctc aaaccggcat tgccgccttt ggccggcatc gcgttaaagc cttcaagggc
      181 gtgtttgtgc aaggtttctt tgcctttttt gatacgcggt gcccaatcgt cttttttgcc
      241 tatgccggga ataccgggaa tcgaaccgcc gtggcacacc tgacaggttg cttcgaagac
      301 ttttttaccg tcaacgccga ccgcaggggc tgccgcaccc ttgtcttctg ccttcgcttc
      361 tgccggagct gcactatcgg caggagcaga agctgttcct gaagcggcat tgtcggcagg
      421 cgcagcctca tcaggattcg ggaaagaacc gccgcttttg ttcgccatgt aagtaatcgc
      481 ccgtttgagt tcctgatcgg tcaggtctgc cgcaccgcct ttggcaggca tggcgttaaa
      541 gccgttcagc gcgtgttgga acaaggtatc gaagccttgc gcgatacgcg gtgcccaatc
      601 gccgttgtgt tccagtttcg gagcgttcgg cacattgctg tccgccgcgt ggcattggat
      661 acagattttg ccgaaaatct gttcgccttg gcgttcgccg acggggatgc cgtcgcccat
      721 cgtcaattgt ccgacaggct ggatacgggt ctgcgttgct gcttccgtag tggcatcgac
      781 atcgccgaac gagccgctgc ccgccagctt aatcaggaaa taaaggactg caataacaat
      841 aacgataccg ctcacaaggg taaacagtgc agagccttgg gctttgttgt cgcggagttg
      901 tttcatttgg taggcctcgc cgtcaggtta ggttgtgctg taaattatag tttggtgtgt
      961 taaacgcagt taacaatatt ttgctggatt atactgaatt cacagggtct ttccaatcgc
     1021 tatcattgaa aatatgaaaa aatttgccaa cggtatctgt ataaaacaaa taatcctttg
     1081 aaaataatgg tttatcctca agaaaactct ccttatgccg ccatacgccg cctgccggcg
     1141 caagataacc tttgccaatt tgcagaattt acgttaacct tacgttttac gcacccatag
     1201 ctcagttgga agagtgtcag tttccgaagc tggaggtcac aggttcgatc cctgttgggt
     1261 gcgccaatta taaagagacc gtctgaaaga taaatatttt tcagacggtc ttttgactta
     1321 cttcaaactc ttatttcaag acttccgcaa atgcgcgggc aacatagtcg gtattcgacg
     1381 tattcagtcc ggcgacgcac atcctgccgg aatccagcag gtaaacggca aattcgtcgc
     1441 gcagcctgcg gacttgttcc acgctcaatc ctgtgtagcc gaacatgccg cgctgtttga
     1501 tgaaataagt gaaatcgcga tcggggattt gcgcagttaa gacatcataa agtttctgcc
     1561 gcatcgcacg gatgcggtcg cgcatcatat aaacctcgtt ttgccacaag gcgtaaagtt
     1621 cggggctgtt catcacgtcg gcggcgatat acgcgccgtg cgcgggcggg ctggagtaga
     1681 tgcggcggac ggtgaatttg agctgtccga acaccaaatc cgcttcttcc ttattcgggc
     1741 aaaccacgct caggccgccg acgcgctcgc cgtagagcga caggtttttt gagaaggaat
     1801 tgctgacgaa caagggcaat tccatttcca ccgctttgcg gacggcgtag gcatcgctgt
     1861 ccaaatcgcc gccgaagcct tggtaggcga tgtccataaa cggaatcagt ttgcgcgttt
     1921 tgatgatgtg caacacttcg tcccattgtt gttccgacat atccacgccg gtcgggttgt
     1981 ggcagcaggg atggaggatc aggacgctgt tttcgggcag ggtgttgaaa aacgcggtca
     2041 tttcgtcgaa tttcacgccg acggcagcag ggtcgtaata tgggtaagtg ccgacctcga
     2101 aacctgcgct ttcaaaaatg ccgcgatggt tgtcccaagt cgggtcgctg acgtaggcgc
     2161 gcgcttcggg aaaccagcgg tgcagaaagt ccgccccgac tttgagcgcg cctgagccgc
     2221 ccaaggtttg tacggtaaca atacgtcctt gcgcgagcgc ggggttgtct ttgccgaaca
     2281 acaaatgctg caccgcgctg cggtaagtgt ctaagccttc catcggcagg tagggcgacg
     2341 gcacaggagt ggcggcgcgg gcggtttcgg cgcggctcac ggattccaac acgggcattt
     2401 tgccttcgtc gtcgaaataa atgcctatgc tcaaattgac tttttcgggg cgcgggtcgt
     2461 ttttgaaggt ttcgaccaaa ctcaaaatcg gatcgccggg atagtattcg atgtgtcggt
     2521 acatagtcct tacctcttgc tttttccaag gattttcttt ttcaacagca caccactttc
     2581 aatatggtgc gtaaacggaa attgatcgaa cagggcggca cgttcaaccg tatgggtttc
     2641 cgccaaggtg tccaaattgg tacgcagcgt ttcgggattg caggaaatgt agatgatgtt
     2701 gtcaaactgc gacaccagct tcaaagtttc ctcatcgata ccggcacgcg gcggatcgac
     2761 gaaaatagtg gaaaatgcgt aatccgtcaa agcaataccg ccatccttaa ggcgtttaaa
     2821 ctcacgtttt ccggtatagg cttcggtaaa ttcttcagca gacagacggg cgattttgat
     2881 gttgccgatg cggttggctt cgatattcca ttgcgccgcg ctgacggagg ttttggagat
     2941 ttcggttgcc aaaacacgtt caaacttttc agataaagga agcgtaaagt tgccgtttcc
     3001 gcaatacagt tcgagcaaat ccccatccaa tccttctgct gcaccgcagg cccattcaag
     3061 cattttctga cacacggcgg cattcggttg ggtaaaactg ccttcaattt gccgataacg
     3121 gaaatcccgg ttgccgacct tcaaagtttc cgttacatag tcctgtttta agactatttt
     3181 ctgtcccctg ctccgcccga tgacggaaat atccaactgt tgctgtaacg cttgcgccgc
     3241 ctgcatccac tcattatcaa gccttttgtg gtaaatcatg gtaaccagca tttccccgct
     3301 gagcgtagac aaaaattcga cggcatacca acggtttctc agttcggcag attgggcggc
     3361 aacggcaatc aattctggca tcaggcggtt gacggcttcg gaagccgctt caaaacggtc
     3421 gcaacggatc aggcttgccc cgccagcctt ttgtcctttt tcaaacatgg cataaaacat
     3481 ttcaccgcct tcgtgccaaa tacggaactc ggcacgcata cggtaatgtt tgtccggaga
     3541 ttcgtacact tcccactcag gaacatccaa acctgcaaaa agggttttaa ggtggtcttt
     3601 tttatcttga agctgctgcg tgtagtcgct catatcgcta tcctgaaaaa tcagaccgga
     3661 ttataagccg attcactgca aaacacgcaa cccctttacc ggcatataac ccgttttcct
     3721 ccaactttta tgctaaaatc cgccgtctcc aacataccgc cgacagaagg aaaatatggc
     3781 aggcaacact ttcggacaac tcttcaccgt gaccacgttc ggcgaaagcc acggcgcagg
     3841 tttgggctgc atcatcgacg gctgcccgcc cggcttggaa ttaagcgaag cggatatcca
     3901 atttgacctc gaccgacgca aacccggcac cagccgccac gttacccaac gccgcgaaac
     3961 cgaccaagta gaaatcctct ccggcgtatt caaaggcaaa accaccggca cgcccatcgc
     4021 cctcttaatc cgcaataccg accagcgcag caaagactac ggcaacatcg cccaaagttt
     4081 ccgccccggc catgccgact atacctattg gcacaaatac ggcacgcgcg actaccgcgg
     4141 cggcggcagg agctccgccc gtgaaaccgc cgcccgcgtt gctgccggag ccgttgccaa
     4201 aaaatggttg aaagaaaaat tcggcacgga aatcaccgcc tacgttaccc aagtcggcga
     4261 aaaagaaatc cggtttgaag gctgcgaaca catttcccaa aatccttttt ttgccgccaa
     4321 ccatagccaa attgccgagc tggaaaacta tatggacagc gtgcgcaaat cgttggattc
     4381 cgtcggcgcg aagctgcata ttgaagccgc caatgtcccc gtcggcttgg gcgaacccgt
     4441 tttcgaccgc ctcgatgccg aaatcgccta cgcgatgatg ggcatcaacg ccgtaaaagg
     4501 cgtggaaatc ggcgcaggtt ttgacagcgt aacgcaacgc ggcagcgaac acggcgacga
     4561 actgaccccg caaggcttcc tgtccaacca ttcgggcggc atcctcggcg gcatcagtac
     4621 cgggcaagac atctgcgtca atatcgccat caaacccacc agctccatcg ccaccccgcg
     4681 ccgcagtatc gacatcaacg gcaaccccat cgaactcgcc acgcacggca ggcacgaccc
     4741 ctgcgtcggc ttgcgcgccg cgccgattgc cgaagctatg ctcgccttgg ttttaatcga
     4801 ccacgccctg cgccatcgtg cgcaaaatgc ggatgttgcc gtggatacgc ccgatatcgc
     4861 ccgctcggac aagtaaaaag atttagccaa aacacgggct ttatactaaa ataccgacca
     4921 ttgccgtgcc gtctgaacgc acgattctta gaggaataca accgaaatga cacaagaaac
     4981 cgctttgggt gcggcactga aatccgccgt ccaaactatg agcaaaaaga aacagaccga
     5041 aatgatcgcc gaccacatct acggcaaata cgatgtgttc aaacgcttca aaccgttggc
     5101 actcggcatc gatcaggatt tgattgccgc tttgccgcaa tacgatgccg cactgattgc
     5161 acgcgtcctc gccaaccact gccgccgtcc gcgctatctg aaagccttgg cgcgcggcgg
     5221 caaacgtttc gatttgaaca accgtttcaa aggcgaagtt acccccgaag aacaggcgat
     5281 cgcgcaaaac catcctttcg tgcagcaggc tttgcaacag cagtccgccc aagctgccgc
     5341 cgaaacgccg tctgttgaag ccgaagcagc cgaatcttcc gcagcagaat aaatccccaa
     5401 atgaaatgcc gtctgaaaac cgatttggtt tcagacggca tttttccgta tgcggcaatc
     5461 acggttcaaa tatcgagttc cgccgtatcg ccttcgcgct ccatccaggc acggcgggcg
     5521 gcggcttcgc ctttgcccat cagtttgacg aagatgtcgc gcgtctcgtc atccgcgcct
     5581 tcggggattt gtacctgcaa caggcggcgg gtgtcggggt gcatggtagt atctttgagc
     5641 tggtctgggt tcatctcacc caaacctttg aaacggctga tggaataagc agtttcttta
     5701 acgccttctt tttgcagccg ctctaaaatg ccgtcaagtt cgttttggtc gagggcgtag
     5761 aatttgcggg cgggtttgct tttaccttgt gcgttgacat ctacgcggaa cagcggcggc
     5821 tgggcgacgt agatgtgtcc gtcagcgacc agtttcggga agtggcggta gaacagggtc
     5881 agcagcaaaa cttgaatatg cgagccgtcc acgtcggcat cggacaggat ggcgattttg
     5941 ccgtaacgca ggccgcttaa atcgggatgg tcgttgatgc cgtgcggatc gacgccgatg
     6001 gcaactgaaa tgtcgtggat ttcggcgttg ccgaagagct ggtcggggtg gacttcaaag
     6061 ctgttgagca ctttgccgcg caggggcagg atggcttggg tggctttgtc gcgggcgagt
     6121 ttggcggagc cgccggcgga atcgccttcg acgaggaaga gttcgttttc gcggatgtct
     6181 tcgctttcgc agtcggtcag cttaccgggc aggacggcga cgccgctgcc ttttttcttt
     6241 tcaatctttt taaccgaacg catccgcgcc tgtgcctgac ggatggcgag ttcggcgatt
     6301 tttttgccga agtccacgtt ttggttcagc cacaattcca aagggtcgcc cgatacggta
     6361 gcaacgagtt tcagcgcgtc gcggttggtc agtttgtctt tggtctgacc ttggaactgc
     6421 gggtcgagga cacgggcaga gaggacaaag gcggttttgc cgaacacgtc gtcgctttgc
     6481 actttcacgc cgcgtggtaa aaggttgtgc agattgatga agttgctgac ggcgttgaac
     6541 acggcttgtt tcaagcccgc ttcgtgcgtg ccgcccagcg gggtggggat gaggttgacg
     6601 tagctttcgt tggcgcacga gccttcttcc agccaggtca gggcaaacgc ggcgccttcg
     6661 ccgatgctga aatcgccgtt gtggccgtct gaaatgtagt tttcgcaaga gaagataggc
     6721 acggcttcct gcgcgtcggc aatcaggtcg gtcagatagc ttttcagacc gtcggggtaa
     6781 tgccaggttt gggtgtgcgc ttcgtcttcg cctttgaccg gacgggtcag ggaaacgcgc
     6841 acgcccggca gcagcacggc tttggcgcgc agcaggcgtt cgagttcggg aatgctgtaa
     6901 ttcgggcttt caaaatattt gccgtccggc cacacgcgca cttcggtgcc gctgtctttg
     6961 acggcgcatt tgcccacttg tgccaacggt tcgaccacgt cgccgccggc aaacacgata
     7021 cggtggattt tgccttcgcg tttgaccgtt acctcaaggc gggtggaaag ggcgttggtc
     7081 acggatacgc ccacgccgtg caggccgcct gaaaaggcat acgcgctgcc gccgtctttt
     7141 ttgttgaact tgccgcctgc gtgcagacgg gtgaacacga gttcgactac gggtacgcct
     7201 tcttcagggt gcagaccgac gggaataccg cggccgttgt cgcgtacgga aagcgaaccg
     7261 tcttcatgaa tttgcacgtc gattgccgta gcgaaaccgc ccaacgcctc gtccgccgcg
     7321 ttatcaatca cttcctgaca gatatgggtc gggctgtcgg tgcgggtgta cataccggga
     7381 cgttctttga ccggttccaa acctttgagg acggtgatgc tggattcgct gtattggttg
     7441 tttttagcca taggaataat ctgaaagtaa gaaaaacaac gctttcagac ggcctgaaag
     7501 cgttgcgttc cgttgtttta ccggttgccg gaaggttggc gggcggcaaa gtcttcataa
     7561 ctttccatac cgcgcaggaa gcgggaagag agttctttca acgcccccaa ataaacgtcg
     7621 cgtttgaact cgatgacttc gtcaacgggc gcccaatatt gatgccaacg ccagccgtca
     7681 aattcggggt ggcgggtggc gcgcaggttg acatcgcaat cccggccggt caggcgcagg
     7741 agataccaaa tctgcttctg cccgcgataa gatccgcgcc attcgcggcg cacccagttg
     7801 ttcggcacgt cgtaacgcag ccagtcgcgc gtccgcccga cgattttgac gtgttgcggc
     7861 agaagtccga cttcttcgta aagctcgcgg tacatcgcgg tttcggggct ttcgcccggc
     7921 ttgatgccgc cttgcggaaa ctgccaagaa tgttcgcgca cgcgcttgcc ccaaaagact
     7981 tcgttacgtt cgttaatcag gataataccg acattggggc gatagccttc cctgtccaac
     8041 acggtgtcgc cctccgttaa attcaatcgt acgattttcc cacaaatcgg gcggttttga
     8101 caaatcagac ggcatggttc aagcatgtcg aaacacagac ggactcggga aaatatctta
     8161 aatttggttt acaataatgt gtttcaaatt attcgggaat cagaccatgt tagatatcca
     8221 attgctccgc agcaacaccg ccgctgtggc ggaacgcttg gcggcgcgcg gttacgagtt
     8281 tgatgccgcg cgtttcaatg cgctggaaga acaacgcaaa gccgtccaag tgaaaaccga
     8341 agaactacaa gcctcgcgta acagcatttc caagcaaatc ggcgcactga aaggtcaggg
     8401 caaacacgaa gaggcgcagg cggccatgga tcaagtcgcc caaatcaaaa ccgatttgga
     8461 acaggctgcc gccgatttgg atgccgttca aaaagaattg gacgcatggt tgttgagcat
     8521 acctaacctg ccgcacgaaa gcgtacctgc cggtaaagac gaaaccgaaa acgtcgaagt
     8581 ccgcaaagtc ggcaccccgc gcgaatttga ctttgaaatc aaagaccatg tcgatttggg
     8641 cgaacctttg ggtttggatt ttgaaggcgg tgcaaaactc tccggcgcac gatttaccgt
     8701 gatgcgcgga caaatcgccc gtctgcaccg cgccttggca cagttcatgc tggatacgca
     8761 cacgctgcaa cacggctaca ccgagcatta cacgccttat atcgttgacg atacgacgct
     8821 gcaaggtacg ggccaactac caaaatttgc ggaagatctg ttccacgtta cccgtggcgg
     8881 cgacgaaacc aaaaccaccc aatacctgat tccgacagcc gaagttaccc tgaccaatac
     8941 cgttgccgac agcattatcc cgtccgaaca actgccgctg aagctgaccg cgcattcgcc
     9001 ctgtttccgc agcgaggcgg gttcgtacgg caaagacacg cgcggtctga ttcgccagca
     9061 ccagttcgac aaagtggaaa tggttcaaat cgttcatccc gaaaaatcat acgaaacgct
     9121 ggaagaaatg gtcggccatg ccgaaaacat cctgaaggct ttggaactgc cctaccgcgt
     9181 gattaccctg tgtaccggcg acatgggctt cggcgcggca aaaacgtatg acttggaagt
     9241 ttgggtgccg gcgcaaaata cctaccgcga aatctcaagc tgctccaact gcgaagattt
     9301 ccaagcccgt cgcatgaagg cgcgtttcaa agacgaaaac ggcaaaaacc gcttggtaca
     9361 tactttgaac ggctccggtt tggcagtcgg cagaaccttg gtcgccgtgt tggaaaacca
     9421 tcaaaacgcc gacggcagca tcaacatccc tgccgcactg caaccgtata tgggcggcgt
     9481 tgccaagttg gaagtgaaat aagccttaac cctgaacata aatgccgtct gaaacttggt
     9541 tcagacggca tttcctttaa acttttaaaa cacgtcagcc gtcggcacga accgcattgc
     9601 cgcaatcgcc ggtctgtccg acctcgcgga tattggacag cgtaacttcc gaaatattac
     9661 ccaacgcctc ttccgtcaaa aatgcctgat ggccggtaaa cagcacatta tgacaagacg
     9721 acaggcggcg gaacacgtcg tcggtaatca catcgttgga tttgtcttca aaaaacagct
     9781 cgcgctcgtt ctcgtacaca tccatgccca atgcgccgat tttccggcgt ttcaacgcct
     9841 caatcgcggc ggcactgtca atcagcccgc cccggctggt gttgataatc atcacgccgt
     9901 ctttcatttt gtcgaacgcc gcttcgttca gcatatagtg gttttccggc gtggaggggc
     9961 aatgcagcgt gatgatgtcc gactgggcat acagctcgtc caaatccaca tattttccgc
    10021 cgagtttttc cacttcgggg ttgcaaaacg gatcgtaagc cagcaggttc ataccgaaac
    10081 cctttaaaat ccgcatcgtt gcgataccga ttttccccgt gccgataacg cccgccgttt
    10141 tgccgtacat attgaaaccg gtcagacctt ccagcgaaaa attcgcatcg cgggtacgct
    10201 gataggcttt gtggatacgg cggttcaacg tcagcatcag accgaccgta tgttccgcaa
    10261 ccgattcggg cgaataggca ggcacgcgca cgactttcaa gcccaactct tcagccgcct
    10321 ttaaatccac attattgaag ccggcacaac gcaacgccac agttttcacg ccaatttgcg
    10381 ccaatttttc caacacgggc cggctgccgt cgtcgttcac aaaaatacag accgcctccg
    10441 caccttccgc cattttcgcc gttttcgcat ccagcataaa atcaaaaaac tccagctcga
    10501 agccgaaatg ccggttggcg cgggtaaaat gttcgcggtc atagcttttc gtaccgtaaa
    10561 tcgcaatctt catcaatatg tccagttgtc gtctatggtt gagaaacggc ataatacact
    10621 gaattcaaac aaatcagtag aatatggtgg attaaaattg attgcatgca cggcatttcc
    10681 atttcaaaac acaaaactca atcgcccatt gccgccagaa gctccgcctg atgctcggca
    10741 atcagggcat cggtgatttc tgccaaatcg ccgtccatca caaaatccag cttgtgcagg
    10801 gtgaggttga tgcggtggtc tgttaccctg ccttggggat agttgtaggt gcggatgcgt
    10861 tcgctgcggt ctccgctgcc gatgagggat ttgcgttcgg cggcttcttt ggcttgggct
    10921 tcgcgttttt gcgcgtcgtt caggcgggcg gcgaggactt tcatcgcctg cgctttgttg
    10981 gcgtgttggc tgcgtccgtc ttggcattcg accaccatgc ctgtgggcag gtgggtgatg
    11041 cggacggcgg aatcggtttt attgatgtgc tgcccgcccg cgccggatgc gcgaaaggtg
    11101 tcgatgcgaa ggtcgacggg gttcaactcg atgtcttcca gttcgtccgc ttcgggcatg
    11161 acggcaacgg tgcaggcgga ggtgtggatg cggccttggc tttcggtggc ggggacgcgc
    11221 tgcacgcggt gtccgcccga ttcaaatttc aaacggctgt acgcgcctaa gccgataata
    11281 cgggcgataa cttctttata gccgcccaac tcgctttcgt tggcggacac gatttcaacc
    11341 tgccagcggt tgcgttcggc gtagcggctg tacatacgca gcaaatcgcc ggcaaacagc
    11401 gcggcttcgt cgccgcccgt tccggcgcgt atttcgatga agatgttttt gtcgtcgtcg
    11461 gtatctttgg gcagcagcag tttttgcaat tcggtatcga gtgcgccgat tttggctttc
    11521 gccgcttcga tttcttcggc ggcaaagtct ttcatttcgg ggtcggacaa catttcttcg
    11581 gcatccgcca aatcgctttg cgccagttga tagttttgga atacttcgac gacgggggtc
    11641 agttcggcgt gttcgcgcgt gagcttgcgg tagttgtcca tatctgccgt ggcttcgggt
    11701 tgccctaaaa ggtgggtaac ttcttccaac cggtcgccga gttgttgcag tttttctaag
    11761 atagacggct tcataattct tccataacaa acgccgcctg aatttcagac ggcatcaaaa
    11821 cgcggtcatt ataatatatt ttctgaatat tcaaaaagat aatctaggga cggagcaggc
    11881 tgtcggcatt gcgttccgtt atcgcggcga tgtccgccac gccgacaccg cgtatctgcg
    11941 cggcaatttc ggcaatccaa agaatattgg caggcgtatt aatctctttt ttcagcataa
    12001 acgggctgtc ggtttccaat acgaaatccc cgtcgttcaa ggctttaagc gtatcgcgca
    12061 ctttacgcgc gttcggattg agcaacaacg aaccgatgcc gattttgaaa cccagtttcg
    12121 tcaacacgcg cgcttcttcc gcgctgcctg agaaggcgtg aacgatgccg ccttgggcaa
    12181 agcctgtctg tttgacggcg gcggcgatgt cggcggtggc tttgagatta tggataatca
    12241 cgcggcggcg cagggtttgc gcgatggcaa gctggcggac gaaaacttgg atttgccgtt
    12301 cgcgctgcgg cggcgtttgg gttttgtcgt aaaaatccaa gccgatttcg ccgacccatg
    12361 cctgcggata acgtgccaac atcgcttcca ggcggacgca atcacgctcg gcaatgccgt
    12421 ctgaaaacca aggatggatg ccgagcgcaa tacggatttg accgtgttcg gacggcattt
    12481 ccgacagttc cgccacgctt tgccaatcct gcggacaggt ggcgggaacg ataaaccgct
    12541 tcaccccaac tttccgcgcg gcggtcagga tgtgcggcag gttttcgcgc agggcggcat
    12601 cggcaaggtg gcagtgggtg tcggtgaagt tcatttcgat tccgcaatgc ggcaggttca
    12661 cataaaaata aggcagggca gcaagggatg ccgtgtacgg gcggcgcata aggcgcggtc
    12721 aacgccccgt cattgccatt tcaatccgct ataacttcag tcttgccaac tctttttcca
    12781 cctcttcctt accccagcct tgcgatacgg cgagggtcat gagtgcggtg gcggtttcga
    12841 ggttgcattt gccgccgttg atgatgcctg agttgcggaa cgcgttgcct tgcgcgtaaa
    12901 cggcagcggt tctgccttgt cggacttggc tgatgttgag cagcagcctg ccttgctgcg
    12961 taaagtcttg cacggcgcgg atgaaacctt cgtctgcggg cgtgttgccg tgtccgtaac
    13021 tttgcaggat aagggcgtgg gcgggaagct gtccgagtcc gtcggcaagt tcttggacgg
    13081 caaagccggg aataagcgtg cggacggcga tttttgcctg cgggtcagga tagcggattt
    13141 tgaggccgtc tgaaacggcc gccgcatctt gggacggaat acgaagattg taccagcctt
    13201 gagtttcatc ccattcggcg atgacaccga aatgcggatt gtcgaagcct gcggcggttt
    13261 cggtgctgac tttgctgctg ccgacggcgg gataaagttt gccgtcaaag gcaataagcg
    13321 tctgcttcaa tccgagtggg aatgcggcaa cggcagtcgc cagattgcgc ggcgcgtcgc
    13381 tgcccacggc gttgtacggc cattgggaac cggtcaggac aatcggtttg accaaaccct
    13441 gcagcgcgag ggcgaacaga ttggcggtat aggcgagcgt gtccgtaccg tgcagcacca
    13501 aaacgccgtc gtactgcggc agcgcggcgg caatgatgtc cagccaagcg caccagtttt
    13561 gcagttggac ggcggaagaa tcaatcagcg tcccacaaac gtgccaatcg aaatccatgc
    13621 cgtttgaaaa cggcgcgagt gcctgcccga ccagcgcggt atcggggcgc aggccttcgc
    13681 tgctttgcgt catgccgatg gtgccgccgg tataaaggac aaaaattttc tgtttcatgg
    13741 ctcatcctga aaatgcaaag accgcctgaa acgcgaaatg cgtccggacg gtcgaaaatc
    13801 tgatttactg ctgcttctgt gccgcttttt tcgctgcctt ttccgccttg cgtttggcgc
    13861 gtttttcgct caattgtgcg cggtaaagct gataatgtcg gcgtttgcgc caccagaacc
    13921 aagccagcgc cgccgccagc acgcccaatg cgatgaagat gccggattgc aggctgtgca
    13981 ttttcgccat cagccaatcg atgttgtgcg cgccgtactc gcccaagtaa atccaaacgg
    14041 gcacggaaat cagcgcggca agcccgtcca taatcagaaa gcgcagatac gatactttgc
    14101 ggctgatgcc ggcggtaacg aaaacggcag tccgcaaacc cggcaggaag cgggcgacaa
    14161 acagaaccca gttgccgtat ttgtcgaatt tttcctgaac ctgtgcgtaa cgtttcggcg
    14221 tcatgatgcg cgcaatcggt ttgaacttga ggattttctg cccccaaatt cgtccggcgg
    14281 cgaacatgat gccgtcgccc gccaacacgc cgagcatacc gactgcaaac ataatatgcg
    14341 gattggtata acccataccc gaaatcacgc cgcctgttac caaggtcaaa tcctcgggaa
    14401 tcggcacgcc gaaaccgcag atgaccaata caaaaaaaac agccgcataa ccgtattcga
    14461 caaaaaaggc ttctaaaaaa gcaaacatgg cggatattcc attgtcggag ataaaaagtc
    14521 agaacaaacc gaaacatttt ctacatgaag caggcattct atcaaagatt atgccgtctg
    14581 aaagcggaaa aaaggcagat tcgcatatcc tccgcatgtt cagacggcat ttaaacagaa
    14641 aaccggccgg ttctgaaaac ggtttttcct gattttgcct aaaggctgcc gatggcggaa
    14701 gcaatacgtt ccgccccttc gcgcgcccaa tcggcctgtc gtgcctcaac catcacacgc
    14761 acgacgggtt cagttcccga agcgcgcaac acaacccttc ctttgccttc gagttctttt
    14821 tccactttgg ccaacacgtc tttcgaagcc tcctgccatt tctgaccctt ttggatgcgc
    14881 acgttaatca tagtttgcgg atacggctgc cagtcggcgc aaacggtggc gaggtcttgg
    14941 ttcaggattt gcagcgccgc caaaacttgc agcgcggaaa taatgccgtc gccggtgtta
    15001 tgtttgtcca tgcacaaaat atggccgctg gcttcgccgc cgatgagcca gccgcgttgg
    15061 ttcaactgtt ccaacacata gcggtcgccg actttggcgc ggcagaaatc gacgccctgc
    15121 tctttcaggg cgatttccat cgccatattg gtcatgaccg tgccgaccac gccgccgatg
    15181 ttgatacctt ctcgggcgcg ggctttggcg atgacgtaaa tcaggctgtc gccgtcgtaa
    15241 accttaccgt ttttatcgac catcatcagg cggtcgccgt cgccgtctaa ggcgatgccg
    15301 tagtcggctt catgctgtaa aacggcggcc tggagtgtct tggtataagt cgcaccgcat
    15361 ttctcattga tattgtagcc gttgggttcg tcgccgatgc tgacaacctg tgcgcccagt
    15421 tcgtgaaaca ccttgggtgc aacaccgtac cccgcgccgt tggcggtatc gacaaccaac
    15481 ttcaaacccc gaaggtcgga atggctgggg aaggtggatt tgcaaaattc gatatagcgg
    15541 tcgtccgcac cgctgatgcg gcgggcgcgt ccgaggcgtg cagaaggctg ggttttcatt
    15601 tcttcgtcga ttttggcttc gatttccaac tcgatttcat cgctcagttt cacgccgccc
    15661 tcggcaaaga atttgatgcc gttgtcggaa taggcattat gcgacgcgga aatcatcaca
    15721 ccggcggaca aacgcagcgc acgggtcaga taagccacgc cgggcgtagg cagcggaccg
    15781 gtttggataa cgttgacacc tgcggcggta aaacctgcca ccaaagcagc ttccagcata
    15841 taaccggaaa tacgcgtatc cttgccgatt aaaacggtcg gtttctggtc ggtgtcgtgc
    15901 tgaaccaaaa cctgacccgc cgcatagccg agtttcaata caaaatcggg cgtaatcgga
    15961 aactgcccga cttcgctgcg cacgccgtcc gtgccgaaat atttttttgc catgtgttgc
    16021 tccgagaatg tgaaccgttg tccgagatta tacagtcagt ttgtgccttg ctgtctgcac
    16081 cgttgatgcc gtctgaaacc gccccgtcct tttcagacgg catgaagtat gtgaaccgct
    16141 gtttacagat tgatgcccaa cgcttcccac accttcaacg catccgctgt cgccttcaca
    16201 tcatgcaccc gcacgatttg cgcgccgcgc gctacggaag ccaacgctgc cgccacgctg
    16261 ccgtgtacgc gttccgccgc atttgcctcg ccggtcagct cgcctatcgt gcttttgcgc
    16321 gatacgccga tgagcagcgg aaaacctgtt tccgccatca attcgggcaa atgccgcatc
    16381 agcgcgatat tgtgttgcaa gggtttgccg aagccggagc cgaagccggg gtcgagtatg
    16441 atgcgttgcg gtgcgatgcc tgccgcgata cattccgctg agcgcgcttt caaataccgc
    16501 gctacttcac cgacaacatc ttgatatttc ggattaatct gcatggtttt gggcaaaccc
    16561 tgcatgtgca tcaggcaaat gcccgtgtcc gcctgacgcg ccagcaattc gaccgcgccc
    16621 tcgtcattca acgccgccac atcattaata atatcgacgc cgccgagtgc caacgctttt
    16681 tccataatca ccgtgcggcg cgtgtccaaa ctgatgggaa cgccccaccc cgccacttcc
    16741 gccaaaacag gctcaacccg cgcccattct tcttcaggcg aaacataatc cgcacccgac
    16801 cgcgtcgatt cgccgccgat gtcgagaatg tccgcaccct ctttcaaaag ccgttcggcg
    16861 tgcgccaaag ctgtttgggc attttgcgaa tacgcgccgc cgtcggaaaa agaatcgggc
    16921 gtgagattca cgatacccat gattttcggt ttgtccaaac cgatttcaaa ccgtcctgcc
    16981 tgccaaacgt gtcgtgccat ctgaactcct cccaaaataa aaaacagatt atatgccgtc
    17041 tgaaaccgtc ttgtgcgctt cagacggcac cgctattcag gcggcagacg gcatgttgtc
    17101 cgaatgtctg ctccgccttt gaatctgccg gtatgcctgc tatccgcccg acttttcaaa
    17161 acaggttccg acgattccgc acgcgcctgc cgcctttgcc aagccgtaca ggatttcctg
    17221 cggcatatcg cggttccata atcccgtaat attcgcaatc acgggcagat ggctgatttg
    17281 gcggactttc acgatggatt cgacatccaa acggtaggga tggtctttgg tatggctcaa
    17341 cacgcccgac tcgcccagaa tcaggtggcg gttgccgcgc gagacaacat attctgcggc
    17401 attcaaccaa tcttcggcag aatggtgctt gtctttacac agcaccaagg gaatgttcag
    17461 gcttccggct tcattcaata cggcaagatc ggacatcagc ccgccgccca aatacaggat
    17521 gtccgccccc gcattcaaag ccgcttcgac atggcggacg ttgcggacgc gcaccaatac
    17581 gggtttccct gcgccatgcg ccgatgcggt ctgttccgcc aaccgtctgc accgtcctcg
    17641 cccttcatcc gcacttgaag tgtcgtataa gtttgccgaa gtgaaaaacg gatctagaaa
    17701 cactgcatcc gcattgcgcc atactgacgg ttctgcggcg atacagacgg tttccccgcc
    17761 gccgaaagcc acgcctttgg cggcaacgcg gctgtcttcc gcccgatttt cccgactgac
    17821 ggttttccat gtatccaaaa tgcggacggc tttctcgacc tccggcagcg tctgcacctc
    17881 cctgacgctc aaaaccctat cgtcgccgat tgcgccgatg acggtacgct cgtcgccgtg
    17941 agaaatgtgt tctcgcagac ctctgctgcg gataaaggcg acaacgccgg caatgtccgc
    18001 ttcggcggca cgcctgctca tgacaataat catatttcct cctgacacaa gaaacggcct
    18061 gcccaaaata ggatttttgc aagccgtgtt atactgtggc gtgttttaca gattgttcgg
    18121 gctatggatt tattatcggt tttccacaaa taccgtctga aatatgcggt ggccgtgctg
    18181 acgatactgc ttttggcggc agtcgggctg cacgcttccg tatatcgcac cttcacgcct
    18241 gaaaacatcc gcagccgcct ccaacaaagc attgcacaca cacaccggaa aatctcgttt
    18301 gatgcggaca ttcagcgcag gctcctgccc cggccgaccg tcatcctgaa aaacctgacc
    18361 attaccgaac ccggcggcga ccagactgcc gtttccgtcc aagaaaccaa aatcggattg
    18421 agctggaaaa acctgtggtc ggatcagata cagattgaaa aatgggtggt ttcgagtgcg
    18481 gaacttgccc tgacgcgcga cgggaaaggt gtttggaaca tccaagacct gatcgacagc
    18541 caaaaacgcc aagcctcagt caaccgcatt atcgtcgaaa acagcaccgt ccgcctcaat
    18601 ttcctgcagg aacagcttat cctgaaggaa atcaacctca acctgcaatc ccccgattcg
    18661 tcggggcagc cgtttgaaag ttcgggcata ctggtttggg gaaagctgtc cgtcccgtgg
    18721 aaaagcaggg ggctgttcct ttcagacggc atcggcacgc ccaaaatctc accgttccat
    18781 tttgaagctt ccacttcgct ggacggacac ggcattacca tttccaccac cggcagccct
    18841 tctgtccgct tcaacgccgg cggagcggat gccgccggcc tcggcctgcg tgcagacact
    18901 tccttccgca acctccacct gaccgcccaa atccctacgc tggcactcag aaataacagc
    18961 attaaaattg aaaccgtcaa cggcgcattt accgccggcg gcgaatatgc ccaatgggac
    19021 ggttcgttca aactcgacaa agccaacctt cactccggca tcgccaacat cggcaacgcc
    19081 gaaatctccg gcagcttcaa aacaccgcgc caccagacca acttctccct caattcgccg
    19141 ctcgtatgga cggaaaacaa agggctggac gcgccgcgcc tgtatgtatc gacccttcag
    19201 gataccgtca accgcctgcc gcaaccccgt ttcatcagcc ggctcgacgg ttcgctgtcc
    19261 gtaccgaatc tgcaaaattg gaatgccgaa ttaaacggca cattcgaccg ccaaaccgtt
    19321 gccgcgaaat tcagatacac acatgaagac gcaccgcatc tagaagccgc cgtcgcactg
    19381 caaaaattga acctgacccc ctatcttgac gacgtgcggc aacaaaacgg caaaatattt
    19441 cccgacaccc tcgccaagct gtccggcgac atcgaggcgc acctgaaaat cggaaaagtc
    19501 caacttcccg gcctgcaact ggacgatatg gaaacctacc tccacgccga caaaggccat
    19561 atcgcgctca gccgtttcaa gtcagggctt tacggcggcc ataccgaagg cggcatcagc
    19621 atcgccaaca cccgtcccgc cacttaccgc ctgcaacaga atgcaagcaa catccaaatc
    19681 caaccgctgc tgcaagacct gttcggcttc cacagcttca gcggcaacgg cgatgcggtc
    19741 atcgacctga ccgcgggcgg cgaaacccga aaagagctta tccgctcgct tcagggcagc
    19801 ctgtcgctga atatttccaa cggcgcgtgg cacggtatcg acatggacaa tatcctgaaa
    19861 aacggcattt cgggcaaaac tgccgacaat gccgcaccca gcacaccctt ccaccgattc
    19921 acgctcaaca gcgaaatttc agacggcatc agccgccaca tcgataccga actcttctcc
    19981 gacagcctct atgttaccag caacggctat accaatctgg atacgcagga attgtctgaa
    20041 gatgtcctta tccgcaacgc cgtccatccg aaaaacaaac cgattcccct gaaaatcacc
    20101 ggtacggtgg acaaaccgtc cattaccgtc gattacggca ggctgaccgg cggcatcaat
    20161 tcgcgcaaag agaaacagaa aatcctcgaa gacaccctgc tggaacaatg gcagtggctc
    20221 aaacctaaag aaccgtaaac atcctgcgta caaaaatgcc gtctgaaaca cccccgcgct
    20281 tcagacggca tgaccgtaaa acctacaacc ccaattcctc ccaaatccca tcaatcttag
    20341 ccgtaaccgc agggtctttt ttgatgacgc gcccccattc gcggtcggtt tctcccggcc
    20401 atttgttggt cgcatccaaa cccattttgc cgccgagtcc gctaacgggg ctggcgaagt
    20461 cgagataatc gatgggcgtg ttttctacca aaacagtgtc gcgcacgggg tccatgcgcg
    20521 tggttaccgc ccagatgact tctttccagt cgcgcacgtt cacatcgtca tccaccacga
    20581 tgatgaattt ggtatacata aactggcgca ggaacgacca gcagcccatc atcacgcgct
    20641 tggcgtgtcc ggcgtactgt tttttcatgc cgatcaccgc catgcggtag gagcagcctt
    20701 cgggcggcag gtaaaaatcg gtgatttcgg ggaactgctt ttgcaaaagc ggtacgaaca
    20761 cttcgttcaa cgccacgccc aaaacagcgg gttcatcggg cggtttgcct gtgtaggtcg
    20821 aatggtaaat cgggttttcg cgcatggtga tgcgctcgac cgtaaataca gggaaatgat
    20881 cctgctcgtt gtaatagccg gtgtggtcgc cgtatgggcc ttccaacgcg gtttcgttcg
    20941 gatgaatcac gccttccaat acaatttcag cacgggcagg cacttgcaaa tcgttgccga
    21001 tacatttcac cagctccgtc cgcgaaccgc gcagcagtcc ggcaaactgg tattcgctca
    21061 aagtatcggg aacaggcgtt accgctccga gaatagtggc agggtcgcaa ccgagcacga
    21121 cggcgacggg atacggcgta tcgggattga gtttgcggaa ctcctgataa tccaacgcgc
    21181 cgccgcgatg cgacagccaa cgcataatca gcttgttttt gccgatgagt tgttggcggt
    21241 aaatgccgag attttggcgt tttttgtgcg gcccgcgcgt tacggtcaag ccccatgtta
    21301 ccagcggcgc aacgtcttcc ggccagcaat gctgaatcgg aagtttatat aaatcaacgt
    21361 cttcgccttc ccacacgatt tcctgacacg gcgcgttttt caccacgttc ggcgccatgc
    21421 tccaaatgtc tttcaagagc ggcagtttgg aaaacgcgtc tttgatgcct ttgggcggtt
    21481 cgggttcttt cagatacgcc agcgtctgcc cgatttcgcg cagcttggac acgctgtccg
    21541 cgcccatgcc catcgccaca cgttcgggcg tgccaaacaa attcgccaac acaggataat
    21601 cgtagcgcgt accgtcgggc ttaatcgggt tttcaaacaa caacgccggc ccttcggcgc
    21661 gcaacacgcg gtcggcgatt tcggtcattt ccaaatgcgg ggaaacagga tgcgcgacgc
    21721 gcttgagttt gccctgttgc tcgagcatag cgatgaagtc gcgcaggtct ttgtatttca
    21781 tattcatcct ttttgtcctt ttatcctgag caatccgatt cggataccgc ccctatcctt
    21841 gcctgcgctt cggcatattc tatgccgtga taaaagtcgc gtaccagcgg atgtccgcca
    21901 ctttgatgaa gttgcagcaa agggcgttgg ttgtcggact gggtaacgac attgcagtgc
    21961 agaccgaagg tgtcggtttc ataagggggc agccagttgc agatcatgcc gaaataaacg
    22021 acgttttccg gattgtcgtg aaagtggctt tgatagtcgt taaaactctt ttcactgaca
    22081 gatacccata ccccatattc cagcgtttcc tcatgcccga taatcggaat aagcagcacc
    22141 gcgcggataa aacggtcggt ttggtcggga tagcggatga tgcagaaatc agaatcacat
    22201 tccgcttgat aagcaatgcg ttcttcttca ctgagttgat tatagggata gggggcggta
    22261 aagccgattg cgggcatatc ttcatgtttt tcgccgcagg aagtgcaagt gtacataagg
    22321 tttcagactt tcaaaacgag tttgcggtaa agccattcgc cggcaaacgg catccccatc
    22381 aatacatagg caatcacgcc ggtataaacc gcccaccaat catatcgccc caaccgtgcc
    22441 aacaaagcgg caagcgtccc gttgattata aaaaatacgc accaaacctg cgttacccgg
    22501 cgggtatagc gcacggcttt ttcaggcagg tcgggctgtt gcagccgcgc aagtttttca
    22561 atcaccgtct gcccggcaaa caggcttccc ccgaacaccg ccaacatcat cagattgacg
    22621 aggacgggat accaatacat tgagtcgcgc cgcccgaaca ccaatactgc ggcaaaaaac
    22681 agtgtaataa acaaagccgc ataacgctgt tggggcaatt tggcagtcag ggcgcgcagc
    22741 agccacaacc cgcacatcgc cgccgccagc cagacaaacc agcccgcctc cctgccgtaa
    22801 tgatagtgga ttaacaaaaa ccagtacagc gttgcctcgc cttgccgtac tatctgcggc
    22861 ttcgtcgcct tgtcctgatt tttgttaatc cactatacca caaagcggga taggcaatgc
    22921 ttaatacggt cagaaaaata tgtccgaaaa aaccgggttt cattttgaat ccgcacaaat
    22981 gttttcagac ggcatccgat aaaaacatgc cgtctgaaaa ataattagaa atacccgatt
    23041 agcccgcctg aatcttcaat accgcctgta ccacgtcgtt gacggtgcgg acattgcgga
    23101 aatcttcagc ctgcagcttg cgggctgcca cagccattca cgattgcgat atacccggcg
    23161 gcattcccga ctgccgttca gcagaaagcg cacccattcc aaaccgctcc aatatgcctc
    23221 gggcagcata ccggcttcag acggcatatc gtccgaagca gataaagtca ggctgtaacg
    23281 cgtccctttg gtcagaacca tcgccaaagc ataagcaaac ggcgcgcgcg ttgccgatac
    23341 ggcatatcct tccggcagcg gatcgtccgc cgccaaaacc aaaaccgacc cgcatccctc
    23401 ttccaacagt gatgccgctt ccgccaatgc cgtttccaca ccgtcggcac acacggccaa
    23461 tgccgtctgc tcgctcatat cccgcctcaa tatcgaccat taccccgccg ttgcattgtg
    23521 caccgacaaa ccgaacgaag tcggcgacac ggtttgcgat ttcaacagtt ccaaccacaa
    23581 atcgaaactg cgcgacattt ccccgtcgtg cgaggcataa actatagtgg attaaattt
//
LOCUS       DAWFAN010000010        22487 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWFAN010000010 DAWFAN010000000
VERSION     DAWFAN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22487
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.010"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..744
                     /locus_tag="WGO65_000289"
     CDS             <1..744
                     /locus_tag="WGO65_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HEZ0326642.1"
                     /translation="TNIVKIDEKLEAVADTVDKHAEAFNDIADSLDETNTKADEAVKT
                     ANEAKQTAEETKQNVDAKVKAAETAAGKAEAAAGTANTAADKAEAVAAKVTDIKADIA
                     TNKDNIAKKANSADVYTREESDSKFVRIDGLNATTEKLDTRLASAEKSIADHDTRLNG
                     LDKTVSDLRKETRQGLAEQAALSGLFQPYNVGRFNVTAAVGGYKSESAVAIGTGFRFT
                     ENFAAKAGVAVGTSSGSSAAYHVGVNYEW"
     gene            complement(810..1148)
                     /locus_tag="WGO65_000290"
     CDS             complement(810..1148)
                     /locus_tag="WGO65_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ0326643.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            1299..5255
                     /gene="purL"
                     /locus_tag="WGO65_000291"
     CDS             1299..5255
                     /gene="purL"
                     /locus_tag="WGO65_000291"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ0326644.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKRQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGADKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTSRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHYPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQQDRDYVDAAFEAAG
                     LTGAVSRIGSPDFDNESISFFGYGYFLEQNRADLQRAWQETSHAIQKLRDNPACADSE
                     FALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKSAS
                     LILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG"
     gene            5350..6108
                     /gene="gloB"
                     /locus_tag="WGO65_000292"
     CDS             5350..6108
                     /gene="gloB"
                     /locus_tag="WGO65_000292"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ0326645.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            6422..10900
                     /gene="mspA"
                     /locus_tag="WGO65_000293"
                     /gene_synonym="ausI"
     CDS             6422..10900
                     /gene="mspA"
                     /locus_tag="WGO65_000293"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ0326646.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLIHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGDSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFVGNNRQQAAEQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNSPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPAPTSHGMPTA
                     PKTGNFSDITGEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYNLSGIDLGFIQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPVT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPPLKLEGDSRGAFQ
                     IHVKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYN
                     PLKEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELAR
                     IDSQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSADICRQVAKAADTNDLTLFETEL
                     DTYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPN
                     LAELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTH
                     RPYQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGEN
                     GALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRS
                     NGNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSAL
                     SVNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            11132..12586
                     /gene="mgtE"
                     /locus_tag="WGO65_000294"
     CDS             11132..12586
                     /gene="mgtE"
                     /locus_tag="WGO65_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ0326647.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLGELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(12672..13580)
                     /gene="hslO"
                     /locus_tag="WGO65_000295"
     CDS             complement(12672..13580)
                     /gene="hslO"
                     /locus_tag="WGO65_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ0326648.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTADELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            13825..14580
                     /locus_tag="WGO65_000296"
     CDS             13825..14580
                     /locus_tag="WGO65_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0326649.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            14599..14817
                     /locus_tag="WGO65_000297"
     CDS             14599..14817
                     /locus_tag="WGO65_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326650.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            14962..15306
                     /locus_tag="WGO65_000298"
     CDS             14962..15306
                     /locus_tag="WGO65_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ0326651.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            15306..15998
                     /locus_tag="WGO65_000299"
     CDS             15306..15998
                     /locus_tag="WGO65_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ0326652.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            16065..17285
                     /gene="argJ"
                     /locus_tag="WGO65_000300"
     CDS             16065..17285
                     /gene="argJ"
                     /locus_tag="WGO65_000300"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ0326653.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            17345..18781
                     /locus_tag="WGO65_000301"
     CDS             17345..18781
                     /locus_tag="WGO65_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0326654.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETTAQTG
                     NAPARPQTAKRECRLKIKTPPIKRRQPP"
     gene            18936..20327
                     /gene="hrpA"
                     /locus_tag="WGO65_000302"
     CDS             18936..20327
                     /gene="hrpA"
                     /locus_tag="WGO65_000302"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ0326655.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     VAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKQRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRISAGV
                     CIRLFSEEDFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            20428..21555
                     /locus_tag="WGO65_000303"
     CDS             20428..21555
                     /locus_tag="WGO65_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326656.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANINEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            21732..>22487
                     /locus_tag="WGO65_000304"
     CDS             21732..>22487
                     /locus_tag="WGO65_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ0326657.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKTSRLLIALFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRHKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSRIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKP"
BASE COUNT         5556 a         6930 c         5520 g         4481 t
ORIGIN      
        1 acaaatatcg taaaaattga tgaaaaatta gaagccgtgg ctgataccgt cgacaagcat
       61 gccgaagcat tcaacgatat cgccgattca ttggatgaaa ccaacactaa ggcagacgaa
      121 gccgtcaaaa ccgccaatga agccaaacag acggccgaag aaaccaaaca aaacgtcgat
      181 gccaaagtaa aagctgcaga aactgcagca ggcaaagccg aagctgccgc tggcacagct
      241 aatactgcag ccgacaaggc cgaagctgtc gctgcaaaag ttaccgacat caaagctgat
      301 atcgctacga acaaagataa tattgctaaa aaagcaaaca gtgccgacgt gtacaccaga
      361 gaagagtctg acagcaaatt tgtcagaatt gatggtctga acgctactac cgaaaaattg
      421 gacacacgct tggcttctgc tgaaaaatcc attgccgatc acgatactcg cctgaacggt
      481 ttggataaaa cagtgtcaga cctgcgcaaa gaaacccgcc aaggccttgc agaacaagcc
      541 gcgctctccg gtctgttcca accttacaac gtgggtcggt tcaatgtaac ggctgcagtc
      601 ggcggctaca aatccgaatc ggcagtcgcc atcggtaccg gcttccgctt taccgaaaac
      661 tttgccgcca aagcaggcgt ggcagtcggc acttcgtccg gttcttccgc agcctaccat
      721 gtcggcgtca attacgagtg gtaagcagca tctcccgata aagaaaccgc agccctgcaa
      781 ggctgcggtt tttattttct atccggccgt cagactgctg cgtccgcacg ttcgcccgtg
      841 cggatacgga ttgcctcctc gaccggcagc acaaaaatct tgccgtcgcc gatttttccc
      901 gaacgcgcca cctcgacaat cacgtcaatc gcgcgttcca cagcgtcatc cgccaacacc
      961 agctcgattt tgaccttggg caggaaatcg acagcgtatt ccgcgccccg gtaaatctcc
     1021 gtatgcccct tctgcctgcc gaaccctttg acctcgctga cggtcatacc cgtaatgccg
     1081 atttccgtca acgcctcgcg cacgtcgtcg agtttgaacg gtttgacaat cgcctcgatt
     1141 tttttcataa aatttccttt gaacaaacaa tacaaacaca tccgaaaaac gggaacctcc
     1201 cgtcagattg tcaacatttt aagccaaaat acccaagcaa tacagccccg ttgcgcgtat
     1261 aatgacagat tttccaaccg catttgagag ccgaatccat gtctgtcgtt ttgcccttgc
     1321 gcggcgttac cgccctttcc gatttccgtg tcgaaaaact cttgcaaaaa gccgccgcac
     1381 tcggactgcc cgaagtcaaa ttaagcagcg aattttggta tttcgccggc agcgagaaag
     1441 cattggatgc cgcgactgtc gaaaaactgc aagccttgtt ggcggcgcaa agcgttgaac
     1501 aaacgccaaa agcgcgcgag ggcttgcatt tgtttttggt aacgccccgt ttgggtacga
     1561 tttcgccgtg ggcttccaag gcgaccaata tcgcggaaaa ctgcggtttg gcaggcatcg
     1621 agcgcatcga gcgcggcatg gcggtgtggc tggaaggtgc gcttaccgat gaacagaaac
     1681 ggcaatgggc ggcgttgctg cacgaccgca tgaccgaatc ggtgctgccc gattttcaga
     1741 cggcatccaa attattccac catctcgaat ccgaaacctt ttccaccgtt gatgttttag
     1801 gcggcggtaa agaggctttg gtcaaagcca ataccgaaat gggtttggca ctctccgccg
     1861 acgaaatcga ttatctggtt gaaaactatc aggctttgca gcgcaatccg tccgatgttg
     1921 aattgatgat gttcgcgcag gcaaacagcg aacactgccg ccacaaaatc ttcaacgccg
     1981 atttcatcct caacggcgaa aaacagccga aatccctgtt cggtatgata cgcgacacgc
     2041 acaacgcgca tcccgaaggc acggtcgtcg cttataaaga caattcgtcc gtaattgaag
     2101 gcgcgaaaat cgagcgtttc tatccgaatg cggcggaaaa ccaaggttac cgtttccacg
     2161 aagaagacac gcatatcatc atgaaagtgg aaacgcacaa ccacccgacc gccatcgcgc
     2221 cgtttgcggg cgcggcaaca ggcgcgggcg gcgaaatccg cgacgaaggc gcgacgggca
     2281 aaggttcgcg tccgaaagca ggcttgaccg gctttaccgt ctccaacctc aacatccccg
     2341 acctcaaaca gccgtgggaa caagactacg gcaagccgga acacatttcc tcgccgttgg
     2401 acattatgat tgaaggcccc atcggcggcg cggcgttcaa caacgaattc ggccgcccca
     2461 acctcttggg ctacttccgc acttttgaag agaagtttga cggtcaggtt cgcggctatc
     2521 acaaaccgat tatgattgcc ggcggcttgg gcagcattca ggcgcagcag acgcataaag
     2581 acgaaatccc cgaaggcgca ttgctgattc aactgggcgg cccgggcatg cttatcggct
     2641 tgggcggcgg cgcggcttct tcgatggata ccggcacgaa cgacgcgtct ttagacttca
     2701 attccgtaca acgcggcaac cccgaaatcg aacgccgcgc gcaggaagtc atcgaccgct
     2761 gctggcagct cggcggcaaa aacccgatta tctccatcca cgacgtgggc gcgggcggtt
     2821 tgtccaacgc cttccccgaa ctcgtcaacg atgccggacg cggcgcggta ttcaagctgc
     2881 gcgaagtgcc gttggaagaa cacggtttaa cgcctttgca aatctggtgt aacgaatcgc
     2941 aagagcgtta tgtgttgtcg attttggaaa aagatttgga caccttccgc gccatctgcg
     3001 aacgcgaacg ctgcccgttt gccgtggtcg gcacggcgac cgacgacggc catttgaaag
     3061 tgcgcgacga tttgttcgcc aacaaccccg tcgatttgcc gctgaacgtc ttgctcggca
     3121 aaccgcccaa aaccacgcgt accgacaaaa cggttacgcc gtccaaaaaa ccgtttcacg
     3181 ccgacgatat cgacatcacc gaagccgcct accgcgtttt gcgcctgcct gccgtagccg
     3241 ccaaaaactt cctgattacc atcggcgacc gcagcgtcgg cggcatgacc caccgcgacc
     3301 aaatggtcgg caaataccaa acccccgtag ccgactgcgc cgttaccatg atgggcttca
     3361 atacctatcg cggcgaagca atgtctatgg gcgaaaaacc gaccgttgcc ctgtttgacg
     3421 cgcctgcttc gggcagaatg tgcgtcggcg aagccatcac caacatcgcg gcggtcaaca
     3481 tcggcgacat cggcaacatc aagctctccg ccaactggat ggcggcgtgc ggcaacgaag
     3541 gcgaagacga aaaactctac cgcaccgtcg aagcggtttc taaagcctgt caggcactgg
     3601 atttgagcat ccccgtgggc aaagacagcc tgtcgatgaa aaccgtttgg caggatggcg
     3661 cggacaagaa atccgtcgtt tcgccgttga gcctgattat ctccgcgttc gcgccggttc
     3721 aagacgtacg caaaaccgtt acgcccgaat tgaaaaacgt cgaagacagc gtattgttat
     3781 ttatcgattt gggcttcggc aaagcgcgta tgggcggctc ggcgtttggt caggtgtaca
     3841 acaatatgag cggcgacgcg cccgatttgg acgatacaag tcgtctgaaa gcgttttaca
     3901 acgtgattca acagcttgtc gccgaagaca aactcttggc ataccacgac cgcagcgacg
     3961 gcggtttgtt tgcgacgctg gcggaaatgg cgtttgcggc acgttgcggt atcagtgccg
     4021 atatagattg cctgatggat aaattcctgc cgattcatta tccggatttc caaggcgacc
     4081 cggccgaaga cttatctgac gaactttata atcatgccgc cattaaaatc ttatttaatg
     4141 aagaattagg tgcggttatc caaatccgcc aacaagatag ggattatgtt gatgcggcat
     4201 ttgaagcggc cggcttaacc ggtgcggtca gccggattgg ctctcctgat tttgacaatg
     4261 aatctatttc tttctttggt tacggttatt tcctagaaca aaaccgtgcc gacctgcaac
     4321 gcgcatggca ggaaacttct cacgccattc aaaaactgcg cgacaacccc gcctgcgccg
     4381 acagcgagtt tgccctgatt ggcgacaacg accgcagcgc attgtttgcc aacctgaaat
     4441 tcgacgtgaa cgaagacatc gccgcgccgt ttatcaacag cggcgcgaaa cccaaaatcg
     4501 ccatcctgcg cgaacagggc gtaaacgggc aaatcgaaat ggccgccgcc tttacccgcg
     4561 ccggtttcga tgcttacgac gtgcatatgt ccgacctgat ggcaggccgt ttccgccttg
     4621 ccgacttcaa aatgctggcg gcgtgcggcg gcttcagcta cggcgacgta ctcggcgcgg
     4681 gcgaaggctg ggcgaaatcc atcctgttcc accctgctct gcgcgaccag tttgccgcat
     4741 tcttcgccga cccggacacg ctgacattgg gcgtgtgcaa cggctgccaa atggtcagca
     4801 acctcgccga gattatcccg ggtgcagagg catggcctaa attcaaacgc aacctgagcg
     4861 aacagtttga agcacgcctg agcatggttc acgtccccaa atccgcgtcg ctgattctga
     4921 acgaaatgca aggctccagc ctgcctgtcg tggtcagcca cggcgaaggc cgcgccgact
     4981 tcgcgcttca cggcggcaac atttctgctg atttgggcat cgcgctgcaa tatgtggacg
     5041 gacaaaacca ggttacccaa acctatccgc tcaaccctaa cggctcgccg caaggcatcg
     5101 ccggtgtgac caacgccgac ggccgcgtta ccattatgat gccgcatccg gaacgcgtgt
     5161 accgtgccgc gcaaatgagc tggaaaccgg aaggctggac ggaactgtcc ggctggtacc
     5221 gcctctttgc cggcgcacgt aaagccttgg gctaaccgcc ctactcaaac caatgccgcc
     5281 tgaagaatat ttcagacggc gttccggcac accatccttt aaacggtatc cgtccaccga
     5341 ggaacactca tgaaaatcac acccgtcaaa gccttgaccg acaactacat ttggatgata
     5401 cagcacggca accatgccgt ctgcgtcgac ccttccgaac cctcgcccgt cttggaattc
     5461 ctcgtccgca accgcctcat gcttgcccaa acatgggtaa ctcaccccca tcccgaccac
     5521 gagggcggtg cggcggcact ctggcgcggc tacatggaat cgcccgttta cggcgaatcc
     5581 gacatcgaag cagcaaccca caccgtaacc gccggcaccc aattcacctt cggcgacgga
     5641 caggttaccg tttgggcaac acccggccac acagaccgcc acaccagcta ccttctcgaa
     5701 acttcagacg gcatacacgt cttttgcggc gacacccttt tttccgccgg ctgcggacgc
     5761 gtgtttaccg gcacaatcga acagctttac gacagcttcc aacgcttcaa ccgcctgcct
     5821 gaaaacaccc tgttctatcc ggcgcacgaa tacaccgccg ccaacctgcg tttcgccgcc
     5881 catatcgagc cggacaacgc cgacattcag acggcactga aagcggcggc gcatacgcct
     5941 accctgcccg ttaccctcgc gcacgaacgc cgcgtcaatc cgtttttgcg cgtcgacctg
     6001 ccgcacgtca gagaccgcgc cgaggcattg agcgggaaaa cgttaaacag cagcctcgat
     6061 acctttgtcg cgctgcgtga acttaaaaac caataccgga cgaaataaaa caacgggaaa
     6121 acgcagccat tcctaggatt tttattaaaa tcttaaataa aatcatacaa tcatcgccaa
     6181 tagacgaaag gacaccgttg ccttataatc aaacaaaaac aaaatatata atatagtgga
     6241 ttgaatttaa atcaggacaa ggcgacgaag ccgcagatag tacggcaagg cgaggcaacg
     6301 ctgtactggt ttttgttaat ccactatatt gttaatccac tatataaatc cagcacaaaa
     6361 cgggatcggt gattcttgtc cgcaagaatc gttgattttc tctattacac ggataatcat
     6421 catgcgcttc acacacacca ccccattttg ttccgtattg tccaccctcg gtctttttgc
     6481 cgtttcccct gcttactcat ccattgtccg caacgatgtc gattaccaat attttcgcga
     6541 ctttgccgaa aataaaggcg cgttcgccgt aggtgcaagc aatatttcca tccaagacaa
     6601 gcaaggcaaa atattaggca gggttctcaa cggcatcccc atgcccgact tccgagtcag
     6661 caaccggcaa accgccatcg ccaccctgat tcacccccaa tacgtcaaca gtgtcaaaca
     6721 caacgtcggc tacggttcca tacaattcgg caacgacacc caaaatccag aagaacaagc
     6781 ctatacctac cgcctcgtat cacgcaaccc gcacccggac tacgactacc accttccccg
     6841 cctcaacaaa ctggttaccg aaatctcacc tgccgcactc agcagcgtac ccttgcttgg
     6901 aaacggccag ccaaaggcca atgcctacct cgataccgac cgcttcccat actttgtacg
     6961 actcggctca ggcacgcaac aagtccgcaa agcagacggc acgcgtacac gaaccgcccc
     7021 ggcataccaa tacctgaccg gcggcacgcc gctgaaagta ttggggttcc aaaaccacgg
     7081 cttactcgtc ggcggcagcc tgaccgacca accccttaac acctacgcaa tcgccggaga
     7141 cagcggttcc cccctgtttg ccttcgacaa gcatgaaaac cgctgggtgc ttgcgggcgt
     7201 actcagcacc tacgccggct tcgataactt cttcaacaaa tacatcgtca cgcaacccga
     7261 attcatccgt tccaccatcc gccaatacga aacccggctg gatgtcgggc tgaccaccaa
     7321 cgaactcata tggcgcgaca acggtaatgg cgacagcacc ctgcaagggc tcaacgaacg
     7381 catcaccctg cccattgcaa acccttcgct tgccccacaa aacgacagca ggcacatgcc
     7441 gtctgaagat gccggcaaaa cgctcatcct atccagcagg ttcgacaaca aaacactgat
     7501 gctggcagac aatatcaacc aaggcgcagg cgcattgcag ttcgacagca acttcaccgt
     7561 cgtcggtaaa aaccacacat ggcaaggtgc aggcgttatc gtagccgacg gcaaacgcgt
     7621 cttctggcaa gtcagcaacc ccaaaggcga ccggctctcc aaactgggcg caggcacgct
     7681 tatcgccaac ggacaaggca tcaaccaggg cgacatcagc atcggggaag gcactgtcgt
     7741 actcgcccaa aaagctgctt cagacggcag caaacaagca ttcaaccaag ttggcatcac
     7801 cagcggcagg ggcacggccg tcctcgccga cagccagcaa atcaaacccg aaaacctcta
     7861 tttcggcttc aggggcggac ggctcgacct caacggcaac aaccttgcct ttacccatat
     7921 ccgccatgcg gacggcggcg cgcaaatcgt caatcacaac cctgaccaag ccgcgacact
     7981 gacgctgacc ggcaaccccg tcctcagtcc cgagcatgtc gagtgggtgc aatggggcaa
     8041 ccgtccgcaa ggcaacgcgg cggtttacga atacatcaac ccgcaccgca accgtcggac
     8101 cgactacttc atactcaaac ccggcggcaa cccgcgcgaa tttttcccgt taaatatgga
     8161 aaactcaaca agctggcaat ttgtcggcaa caacaggcaa caggccgccg aacaagtcgc
     8221 ccaagccgaa aatgcccgcc ccgacctgat taccttcggc ggatacttgg gtgagaacgc
     8281 gcaaacgggt aaagccgcac cgagttacag caaaaccaat atcgaatggg tgaatagtcc
     8341 aaacacccca cacaagccct cgccctatac gaatacgcca acccgcacag gggcaggcgt
     8401 accgacgatt accgcctcaa agaaggcggc aaccccggcg cctacttccc atgggatgcc
     8461 tacagcaccc aagactggga atttctcgga cataaccggc gaagcagcca tagaaaaaac
     8521 ccgccatatc gcaaatgccg ccgtatacgg ccggcccgaa taccgttaca acggcgcact
     8581 caacctgcac tatcgtccca aacgcaccga cagcacgctg ttgctcaacg gcggcatgaa
     8641 ccttaacggg gaagtcttga ttgagggcgg caatatgatt gtgtcaggca ggcccgtacc
     8701 ccatgcctac gaccaccagg ccaaacgcga acccgttctt gaaaacgaat ggaccgacgg
     8761 cagcttcaag gctgcacggt tcaccctgcg aaaccatgcc cgactgacgg cagggcgcaa
     8821 taccgcgcat ctggacggcg acataaccgc atacaatctg tccggcatcg acctcggctt
     8881 tatccaaggc aaaacaccgg aatgctaccg ctcctaccat agcggcagca cccactgcac
     8941 acccaacgcc gttttaaaag ccgaaaacta tcgtgcacta cctgtaacgc aagtacgcgg
     9001 cgacattacc cttaacgacc gttcagagct ccgcctgggc aaagcacacc tgtacggcag
     9061 catccgtgcc ggcaaagaca ccgcagtccg catggaagca gacagcaact ggacactttc
     9121 ccagtccagc cacaccggcg cactgacgct tgacggcgca caaattaccc tgaaccccga
     9181 tttcgccaat aatacacaca acaaccgctt caacacactg accgtcaacg gcacacttga
     9241 cgggttcggc acattccgat tcctgaccgg catcgtccga aaacaaaatg cccccccccc
     9301 cctcaaactg gaaggggaca gccgcggcgc attccaaatc cacgtcaaaa acaccggaca
     9361 agaacctcaa acaaccgaat cgcttgcact tgtgagcctc aatccgaaac acagccacca
     9421 agcccgattc accctccaaa acggctatgc cgatttgggt gcctaccgct acatcctccg
     9481 caaaaacaac aacggataca gcctgtacaa cccgctcaaa gaggccgaac ttcaaattga
     9541 agccacgcgt gcggaacatg agcgcaacca acaggcatac aaccaattac aggcaaccga
     9601 catcagcaga caggttcaac atgactctga cgcgaccagg caggcactac aggcctggca
     9661 gaacagtcaa accgaacttg cccgcatcga cagccaagtc caatatctgt ccgcccaatt
     9721 gaaacagaca gacccgctga ccggcattct gacgcgtgcc caaaacctgt gtgccgcaca
     9781 aggatacagt gccgatatct gccgtcaggt tgccaaagcc gccgacacga acgacctgac
     9841 actcttcgaa accgaactgg atacgtatat agaacgtgta gaaatggccg aatccgaact
     9901 tgacaaagca cggcaaggcg gcgatgcgca agccgtcgaa acagcccggc acgcctacct
     9961 gaacgcactc aaccgtctgt cccgacaaat ccacagtttg aaaaccggcg ttgccggcat
    10021 ccgtatgccg aacctggccg aactgatcag ccggtcggcc aacaccgccg tttccgaaca
    10081 ggccgcctac aataccggcc ggcaacaggc gggacgccgc atcgaccgcc accttaccga
    10141 tccgcagcag caaaacatct ggctggaaac cggtacgcaa caaaccgact accatagcgg
    10201 cacacaccgt ccctaccaac aaactaccaa ctatgcacat atcggcatcc aaaccggcat
    10261 caccgaccgt ctcagtgtcg gtacgatttt aaccgatgag cgcacaaaca accgttttga
    10321 tgaaggcgta tccgcccgaa accgcagcaa cggcgcacat ctgttcgtca aaggggaaaa
    10381 cggcgcactc tttgccgcgg cagatttagg ctacagcaac agccgtaccc gatttaccga
    10441 ttatgacggg gctgccgtcc gccgccacgc atgggatgca ggcatcaaca ccggcatcaa
    10501 aatcgatacc ggcatcaacc tcagacccta tgccggcatc cgtataaacc gcagcaacgg
    10561 caaccggtac gtactcgacg gcgcagagat aaacagcccg gcgcaaatcc aaaccacatg
    10621 gcatgccggc atccgtctcg ataaaaccgt cgaactgggt caagccaagc tgacccccgc
    10681 tttcagcagc gattactacc atacccgcca aaacagcggt tccgccctca gcgtcaacga
    10741 ccgtacctta ctgcagcaag ccgcccacgg cacactgcat accctgcaaa tcgacgccgg
    10801 atacaaaggc tggaacgcca aacttcatgc cgcttacggc aaagacagca acaccgcccg
    10861 ccacaaacag gcaggaatca aaataggcta caactggtaa caagccgata aaaatgccgt
    10921 ctggaacccg cgtttcagac ggcatttgcg ttaaaaatag taaaccgttc caaaagggag
    10981 tagaatagtg ccgtttccaa ccctgcgcct gtaccgtcag gcttttatta tggaccttcc
    11041 cagttcgttt ttactgaacg ccccttccga ttccaaacga caataaccat cccgccggaa
    11101 tgcctccccg cacacgacgg gcggagcatt tatgagcatc gaacacactc ctccgacaca
    11161 cgacggcgaa accggtcaaa accatgccga acgcccttcc gctgatttcg accgtgtcca
    11221 ctccctctgc gaaatcctcg aacctgcctt tgaacaaatc gaaaacggca caccgctcga
    11281 agacgcgccg ctgcgcgaca agctgaccga gctgaccgtc ctcttgggcg agctgcaccc
    11341 tgccgacgtg gcggcggtgt tggaatcgct gccgccgcgc gaacgcaacg tcgtttggct
    11401 tctggtcaaa cccgaagacg acggcgaagt attgctggaa gtatccgacg cggtgcgcga
    11461 aacactgatc gagtcgatgg ataaggacga attgttggcg gcagttgacg atttggacgc
    11521 ggacgaattg gcggaactgg cagacgattt gccgcaccaa gtggtttacg aagccttaca
    11581 gacgcgtgat gaggaagaac gcgcccaagt caaggcggca atgtcctacg aagacaacca
    11641 agtcggtgcg attatggact tcgagttggt cagcatccgc gccgatgtcg cctgcgaagt
    11701 ggtgctgcgt tacctgcgcc gcttcgacag cctgcccgac cacacggata aaatcttcgt
    11761 ggttgacgaa aacgatgtgt tgcagggcgt gctgcccatc cgcaaacttt tggtcgccga
    11821 tcccgaagac ttggtggaaa acgtgatggc gaaagatgtc gtgcgtttcc gcgccgaaga
    11881 cgacgtggaa gaagcagcgc aggcgtttga acgctatgac ttggttaccg cgcccgtcgt
    11941 cgatgaaaac aaaaagctca tcggcagggt gaccgttgac gaaatggtgg acgtgatccg
    12001 cgaagaatcc gaagcagata tgttgaacat ggcgggtttg caagaagagg aagacttatt
    12061 cgcccccgtc ttggattccg tcaaaaaccg ctggatgtgg ctcgccgtca acctctgcac
    12121 cgccttcctt gccagccgcg tcatcggcgc gtttgaaggc agcatcgaaa aaatcgtcgc
    12181 actcgccgcg ctgatgccca tcgtcgccgg tatcggcggc aactcgggca accagacgat
    12241 taccatgatt gtccgcgcca tggcgatggg gcaactgacg gatatgcagg cggggcgttt
    12301 gctgaaaaaa gaagtcggcg tcgccttggt caacggcatc atttggggaa cggtcatggg
    12361 cgcagtatct tggctgcttt acggcagcct cggcatcggt ttggtcatgg ttgccgcgat
    12421 gacgctcaac ctgctgctgg cggcaaccgt cggcgtatta attcccgtgg taatggaaaa
    12481 gttcggacgc gatcccgcgc tgggcagctc ggtgctgatt acggcggtaa cggattccgg
    12541 cggcttcctg attttcttgg gtctcgccac cctgttcctg ctttaaatgc cgtctgaacc
    12601 cgcgcaaaaa tgccgtctga agcggaagct gcttcagacg gcatttgact atttatcctt
    12661 gttgcacaag attattggac ggtatgccgg ggcagccctt tggcaacgcc gaccacatcc
    12721 tccccgaaca gcgcgttgac atcggtttcg tcaaacacat atttgctgtg gcagaaatcg
    12781 caatcgactt cgatgctgcc ttgttccacc accacgccgc cgacttcttc cccgcccagc
    12841 atcagcagca tatcgctgac tttgccgcgc gaacaggtgc atgaaaattc aaacgtttcc
    12901 ggctcgaaca cgcgcggcgg cgtttcgtgg aacaggcggt acaaaacatg ttgcgcatcc
    12961 aattctgcca gctcgtccgc cgtcagtgta cgcgccagcg tactgacgtg ttcccatgcc
    13021 tcttcatcca acaccgcttc aggcagacgc tgcaccagca gaccgcccgc cgcttcgtcg
    13081 cttgcagaca ggacgatgtg cgtatcgatt tgttcggaac gcttcatata gttcgccaac
    13141 atttgcgcga taccgccgcc ttccaaaggc actacgccct gccagggttc gccgtctttg
    13201 ggctgcagcg tcagcacgaa tacgccgccc tcgcccaaaa ggtcgccgag gctttcgtca
    13261 tcggctattt ctgcggtttc gtcccaacgc gcggttgcac ggacggtacg gtcggaagcc
    13321 gcttccgcaa ccagcatttt cagccgcccc cgcccctgaa cctgcacaat cagcgtgcct
    13381 tcgtttttga ggttgcccga cagcagaaca ccggcagcca acagctcgcc caaagcgcgg
    13441 cggatggcgg cgggatagtt tttctgtttt acaatgtgct gccacacgtt ttccagacgg
    13501 acgtgcagcc cgcgcacggg catatcgtcg aagataaagc gggtacgcac atcggcgcgg
    13561 ttgatggcgg tttgattcat gattttctct gactgattgt tcggatggcg gctatatggt
    13621 tgcggtcggc gcgaaaacaa gacggacggc ggatgcgctt cccaaattat caataaatta
    13681 tataaaaatc aacatattaa ctcaatctaa caagccgttt tttgccaaac agccgttttt
    13741 ttatatacaa tcaacaagat attttcgact gatacagcat aacatcgcac ggcggcacga
    13801 tgcctcctgc gcggaaacac cgatatggat tcttttttca aaccggcagt ttgggcggtt
    13861 ttgtggctga tgtttgccgt ccgccccgcc cttgccgacg agttgaccaa cctgctcagc
    13921 agccgcgagc agattctcag acagtttgcc gaagacgaac agcccgtttt acccatcaac
    13981 cgagcccccg cccggcgggc gggcaatgcc gacgaactca tcggcagcgc gatggggctt
    14041 aacgaacagc ccgttttacc cgtcaaccga gtccccgccc ggcgggcggg caatgccgac
    14101 gaactcatcg gcaacgcgat ggggcttaac gaacagcccg ttttacccgt caaccgagcc
    14161 cccgcccggc gggcgggcaa tgccgacgaa ctcatcggca acgcgatggg acttttgggt
    14221 attgcctacc gctacggcgg cacatcgatt tctaccggtt ttgactgcag cggcttcatg
    14281 cagcacatct tcaaacgcgc catgggcatc aacctgccgc gcacgtcggc agaacaggcg
    14341 cggatgggta cgccggttgc ccgaagcgaa ttgcagcccg gggatatggt gtttttccgc
    14401 acgctcggcg gcagccgcat ttcccatgtc ggactttata tcggcaacaa ccgcttcatc
    14461 cacgcgccgc gcacggggaa aaatatcgaa atcaccagcc tgagccacaa atattggagc
    14521 ggcaaatacg cgttcgcccg ccgggtcaag aaaaacgacc cgtcccgctt tctgaactga
    14581 tttcccaagg aatacgcaat gagtatgccc gaaatgccca aatggtacga cgatgacgga
    14641 cagatcgtgt cctgtaccga aaaggtcaaa gtgatgtccg aaaatatggc cgagctgtat
    14701 cagacggcac aagacgcgtt tgaagacgcg ctgctgatgg gttgcggcga acgtcagttg
    14761 cgcgattacc tgctcgcgct gattgaaggt ttggaaaatc cctaccgcaa agtctgaaca
    14821 cgccccggtt gctgcggcac ggtttatccg tgccgttttt gcgtttgtgc gcggcttcgg
    14881 cttttcagac ggcatatttg acgttatgat taaacagtta acaagattta tcacaacgcc
    14941 gtcaaaagac agacacacaa catgaacatc atccgcgcgc tcctcatcat cctcggctgc
    15001 ctcgccgccg gcgaaaccgc cgttttccta gcaggcatca aactgcccgg cagcatcgtc
    15061 ggcatgggcg tgctgtttgc gcttttgcag gcaggttggg tcaaaacgtc ttggctacaa
    15121 cagcttaccg acgcgctgat ggcgaatctg acgctgtttc tcgtgccgcc ctgcgtggcg
    15181 gtcatcagct atttggattt gattgccgac gattggtttt cgatactggt ttccgcctcc
    15241 gccagcactt tgtgcgtact gctggttaca ggcaaggttc accgctggat acggagcatt
    15301 atctgatgaa cgaaatcctc aggcagccca gcgttctgct tttcctcacg cttgccgtgt
    15361 acgcgcttgc gattatcgtg cgcacgcgca cgggcaatat cttctgcaac cccgtactcg
    15421 tcagcactat cgtgctgatt gcctacctga aaatcctcga catcgattat gcggtgtacc
    15481 acaacgccgc gcaattcatt gatttttggc tgaaacccgc cgtcgtcgtg cttgccgtgc
    15541 cgctctacca aaaccgccgt aaaatcttca accagtggct gcccgtcatc gtttcacagc
    15601 ttgcgggcag cgttacgggc attgttacgg ggatgtattt tgccaaatgg ctgggcgcgg
    15661 aacgcgaagt cgtcctctcg ctcgcgtcca aatctgttac caaccccatc gccattgaaa
    15721 tcacccgctc catcggcggc attcccgcca ttaccgccgc caccgtcatc attgccggtc
    15781 tggtcggaca gattgccggt tacaaaatgc tgaagaacac agtcgtcatg ccctcgtccg
    15841 tgggtatgtc gctcggcacg gcttcgcacg cgatggggat tgccgcctcg ctcgaacgca
    15901 gccgccgcat ggcggcatac gcgggactgg ggctgacgtt caacggcgta ctgaccgcgc
    15961 tgattgcgcc gctgctcatc cccgttttgg gattttgaac ccgtttcaga cggcatttca
    16021 gcccatgctg tctgaacgcc gacacactcg caaggagaac cgttatggct gtcaacctga
    16081 ccgaaaaaac cgccgaacaa ctgcccgaca tcgacggcat tgccctctac accgcccaag
    16141 caggcgtgaa gaagcccggg cataccgacc tgacactgat tgccgtagcc gccggcagca
    16201 ccgtcggtgc agtcttcacg accaaccgtt tctgtgccgc gcccgtccac atcgccaaat
    16261 cgcacctttt cgacgaagac ggcgtgcgcg ccctcgtcat caacacgggc aacgccaacg
    16321 cgggtacggg cgcacagggc agaatcgatg ctttggcagt gtgtgccgcc gccgcccggc
    16381 aaatcggctg caaaccgaac caggtgctgc ccttctccac cggcgtgatt ctcgaaccgc
    16441 tgcccgcaga caaaatcatc gccgccctgc ccaaaatgca gcctgccttc tggaacgaag
    16501 cggcacgcgc catcatgacc accgacaccg tgcccaaagc cgcctcgcgc gaaggcaagg
    16561 tcggcgacaa acacaccgtc cgcgccacgg gcatcgccaa aggctcgggc atgattcatc
    16621 ccaatatggc gaccatgctc ggtttcatcg ccaccgatgc caaagtttcc caacccgtcc
    16681 tccaactgat gacgcaggaa atcgccgacg aaaccttcaa caccatcacc gttgacggcg
    16741 acaccagcac caacgacagc ttcgtcatca tcgccaccgg caaaaacagc caaagcgaaa
    16801 tcgacaacat cgccgacccg cgttacgccc aactcaaaga attgttgtgc agcctcgcgc
    16861 tcgaactcgc ccaagccatc gtccgcgacg gcgaaggtgc gaccaagttc atcaccgtcc
    16921 gcgtcgaaaa cgccaaaacc cgcgacgaag cccgccaagc cgcctacgcc gtggcacgtt
    16981 cgccgctggt caaaaccgcc tttttcgcct ccgaccccaa cctcggcagg ctgctcgccg
    17041 ccatcggtta tgccggcgtt gccgacctcg ataccgacct cgtggaaatg tatctcgacg
    17101 atattttggt tgccgaacac ggcggacgcg ccgcaagcta caccgaagca caagggcagg
    17161 cggtaatgtc gaaggccgaa atcaccgtcc gcatcaagct gcatcgcgga caagccgccg
    17221 ccaccgtcta tacctgcgac ctgtcgcacg gatacgtttc catcaacgcc gactaccgtt
    17281 cctgacccga cacggcttca gacggcatac ataaaattcc gtctgaaccg ccggacaaca
    17341 taccatgacc tccacattcc cccgccgcct cgcccgcaaa atccgccaaa cccgccgcct
    17401 gtcgcgcaaa agcatcgcct ttctgttcct tttggcaggt tcggcactcg tcgccctgac
    17461 cgcgctgttt tttgcccatc ttgccgattt tgcgctggaa ctgaacgcca aactggttca
    17521 acaataccca tggttcgcgt gggtcgcgct tcctttgggt ttgccgctta ttgcgtggct
    17581 cacacgcaaa ttcgccccct tcaccgccgg cagcggcatc ccgcaggtca tcgcctcact
    17641 gtcgctgccc tacggcgcac agaaaacgcg gctgatccgc ctcgggcaga cactgctgaa
    17701 gattccgctg acctttttgg gtatgctgtt cggcgcgtcc atcggacgcg aaggtccgtc
    17761 cgtgcaggtc ggcgcggcag tgatgggcgc gtggggcgcg tggtgcaaga aacacggctt
    17821 ggcattcaaa gggatgcagg aaaacgattt gatggcggcg ggcgcggcgg gcggtttggc
    17881 agccgcgttc aacgcgccgc tggcgggcgt gattttcgcc attgaggaac tcgggcgcgg
    17941 catcatgttg cgctgggaga ggcaaattct tttgggcgtg ctcgcctccg gtttcataca
    18001 ggtcgccatt cagggcaaca acccgtattt ttccggcttc aacggcggcg tattggaaca
    18061 tatctttctg tgggtcgcac tgtccggcct ggtttgcggc gcggcgggcg gcctgttcgg
    18121 acgtttgctc tatcgcggtg cggcggcgtt tgcaccgcgc aagatacgcg gcttcatccg
    18181 caaccgtccg ctgctgctgg cggcactgat ggggctgctg ctcgccctgc tcggcacgtt
    18241 ctaccaaggc aaaacctacg gcaccggcta ccacgaagcc gcccaagccc tgcacggcat
    18301 ctatgaagcc cccttcggac tcgccgccgc caaatggctc gccaccgtat tcagctattg
    18361 ggcaggcgtt ccgggcggca ttttcactcc ctcgctgacc ataggcgcgg ttttgggcga
    18421 gcatatcgcc gccatcgccg acatatcgca gggtgcaaac atcatcgtcc tcatctgcat
    18481 ggcggcattt ctggcgggcg cgacacaatc cccgattact tccgccgtcg tcgtcatgga
    18541 aatgacgggc ggacaaagcc tgctgttttg gatgctaatt gcctgcattt tcgcctcgca
    18601 ggtttcgcgc cagttttcgc cgcgtccgtt ctaccacgca tcgggaatgc gcttccgcca
    18661 gcgcgtgctt caggaaacca ccgcccaaac cggcaatgcg cccgcaagac cgcaaacagc
    18721 aaaacgggaa tgccgtctga aaattaaaac gcccccgatc aaacgccggc agccgccttg
    18781 atttgaatac cgttccgccg ctgcttgaaa cttcaacaac aatgccgtct gaacgacaga
    18841 atgcggtttt cagacggcat ttccccatcc cgatattgcc taaacaaaac cgaagcgttt
    18901 gctataattg cactttttac cgcacacgca cgcccatgtt tcccgatttc tcccaaaccc
    18961 tctccaaaga ccgccacttc ctgcaatccg ccttcaaaaa tcccaacaaa tacggcggct
    19021 tgtccaaagt cgaagaaaaa taccgaaaat cgcacgaaat ctttttgaaa cgtttggcag
    19081 ccttgcccaa acccgaattc gacaacaccc tgcccgttca cgaaaaactt gacgaaatca
    19141 aaaaaaccgt tgccgaacat caggtaacga ttatttgcgg cgaaaccggt tcgggcaaaa
    19201 ccacgcagtt gcccaagatt tgtttggaac tcgggcgcgg ggtagcgggt ttgatcgggc
    19261 atacccagcc gcgccgtttg gcggcgcgtt cggtggcaga gcggattgcc gaagagctga
    19321 aatccgaaat cggcagcgcg gtcggctata aagtacgctt caccgaccac acctcgcgcg
    19381 atgcctgcgt caagctgatg accgacggca tcctgctggc ggaaacgcag accgaccgtt
    19441 atctcgccgc ctacgacacg attatcatcg acgaagcgca cgagcgcagc ctgaacatcg
    19501 acttcctttt gggctatttg aaacaactcc tgccgcgccg ccccgatttg aaagtcatca
    19561 tcacctcggc aacgatagac gcagaacgct tctcccgaca cttcaacggc gcgcccgttt
    19621 tagaagtgag cggacggacg tatcccgtcg aaatcctcta ccgaccgctg accggcaaag
    19681 acgaagacga cgcagaagtg gagttgaccg acgcgattgt cgatgccgcc gatgaattgg
    19741 cgcgatatgg cgaaggcgat atcttggtat tcctgccggg cgaacgcgaa atccgcgaag
    19801 ccgccgaagc cctgcgcaaa tccacgctgc gccgcaacga cgaaatcctg cccctgttcg
    19861 cacgcctgtc gcacgccgaa cagcacaaaa tcttccaccc ctccggcgca aaacaacgca
    19921 tcgtgctggc aaccaacgtc gccgaaacct cgcttaccgt gccgggcatc aaatacgtca
    19981 tcgacaccgg tctcgcacgc gtcaaacgct attccgcacg ggcgaaagtg gagcagcttc
    20041 atatcgaaaa aatctcccaa gccgccgccc gccaacgctc cggccgctgc ggacgcatct
    20101 ccgcaggcgt atgtatccga ctgttttcag aagaagattt caacaaccgc cccgaattta
    20161 ccgaccccga aatcgcccgc agcaacctcg ccgccgtcat cctgcgcatg gcatcgctga
    20221 aactcggcga cgtagcggca ttcccatttt tagaaatgcc cgattcgagg tatattaatg
    20281 atgggtttca ggtgttgctg gaattagggg cagtggaggc cgcctgaaaa caagtaggat
    20341 atacacttct acctgacaaa gaccaacaaa cgataaaact acggcacatc tagatgccca
    20401 acctacaaca ttgcacacgg taaaaacatg aaacacttac tcatcgattt tgaaaacgtc
    20461 cagccgcaaa acttagacaa attactaacc gaaaataccc atatttggct atttataggt
    20521 gtattacaca aaatgttacc tattagtctg gtgcaatccc tactacgttt cggcgaacgt
    20581 gtccatcttg tccagttaca aaaaacgggg aaaaacgcat tggattttta cctgtcctat
    20641 tacctcggac aaattaccgc cacagacccc aatgcccaaa tcggcatact ctcgcgtgat
    20701 ggaggatacg atgttctggt cgaacatatt ttgaaaaacc accaagcgaa gggtatcgtg
    20761 cgcctagcca atataaatga agtacaacat cagaaaatta ctaccgaacc gccgtcagca
    20821 ttgctggaaa acactcctca gcctgaaacc acactcaaac cacagcaacc attaacttcc
    20881 tatttccaag cagccctaac tgcactgcgc cgccccgacg ctttccgccc ctgccgcctg
    20941 cataacctgc gacaaaatct gcgtaagcat attttgagtg atttgtttaa agaaaaaacc
    21001 gatgaagaat gcgaaataac cactgctaac gttatcaata aactcaaagc acaaaacttc
    21061 atcagcattg atgaacagga aaccgtttcc taccatctca gtgataatga tttgttacaa
    21121 agaatccaac gccatatttt aagccaacgt cccaaaacct acgctgattt tcaagccgtc
    21181 gtgcaaaacc gagcagatgc acttcactta acagtcggta ccaacgacat tcaatccttt
    21241 gcgcgacatt tgcgcgacca aaacctgatc cgccaaaaca atgggaaaat tgaatatgca
    21301 ccgtttactg aacctaaacc acagccaaca cccaagcagc ctaaaaaaac cgcatgggaa
    21361 cctgatgaaa ttatttggaa aaaagtgatt gccgcgttat cgttaaagaa ccgtcctaat
    21421 aaaaccaaaa ctttacgcaa tacaatccag gcactcacaa aatccaatgc acaagaaact
    21481 gacaaactgc tacaacattt acaagatacc caagtcctac gtattgacgg tactaaaatt
    21541 gtgtatatca agtaggatgg gcatttatgc ctgacctaca aaaccaaaaa caacctaaca
    21601 cccttaatca actccacaaa cccttttcag acaacctcgt tttttgaaaa acaatctgta
    21661 aacagataac tgctgaagaa taccgttgcc gagccccaaa acctgcctgc aacttttatt
    21721 gtgaactgcc cattatgaaa aaatcccttt tcgttctctt tctctattca tctctactta
    21781 ccgccagcga aatcgcctat cgctttgtat tcggaattga aaccttaccg gctgcaaaaa
    21841 tggcagaaac gtttgcgctg acatttgtga ttgctgcgct gtatctgttt gcgcgttata
    21901 aaacttcgcg gctgctgatt gcgctgtttt tcgcgttcag cattattgcc aacaatgtac
    21961 attatgcggt ttatcaaagt tggatgacgg gtattaacta ttggctgatg ctgaaagagg
    22021 ttaccgaagt cggcagtgcg ggcgcgtcga tgttggataa gttgtggctg cctgcgttgt
    22081 ggggcgtggc ggaagtcgtg ctgttttgta gccttgccaa gttccgccat aagacgcatt
    22141 tttctgccga tatattgttt gccttcctaa tgctgatgat tttcgtgcgt tcgttcgaca
    22201 cgaaacaaga gcacggtatt tcgcccaaac cgacatacag ccgcatcaaa gccaattatt
    22261 tcagcttcgg ttattttgtc ggacgcgtgt tgccgtatca gttgtttgat ttaagcaaga
    22321 ttcctgtgtt caaacagcct gctccaagca gaatcgggca aggcagtatt caaaatatcg
    22381 tcctgattat gggcgaaagc gaaagcgcgg cgcatttgaa attgtttggc tacgggcgcg
    22441 aaacttcgcc gtttttaacc cggctttcgc aagccgattt taagccg
//
LOCUS       DAWFAN010000011        22449 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWFAN010000011 DAWFAN010000000
VERSION     DAWFAN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22449
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.011"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            45..608
                     /locus_tag="WGO65_000305"
     CDS             45..608
                     /locus_tag="WGO65_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0326658.1"
                     /translation="MRHLENNGYANVAGLERILAVKTDNYKEKENLLHEIFSKSRIGD
                     TELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSVHERRQEGNAGSGRKQLLDLV
                     RRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGKSGTRLTDEIADGIHIYTCFS
                     RADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            645..1406
                     /locus_tag="WGO65_000306"
     CDS             645..1406
                     /locus_tag="WGO65_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ0326659.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            1461..1802
                     /gene="fdx"
                     /locus_tag="WGO65_000307"
     CDS             1461..1802
                     /gene="fdx"
                     /locus_tag="WGO65_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ0326660.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(2089..2676)
                     /locus_tag="WGO65_000308"
     CDS             complement(2089..2676)
                     /locus_tag="WGO65_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ0326661.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRTATSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            2889..3086
                     /gene="iscX"
                     /locus_tag="WGO65_000309"
     CDS             2889..3086
                     /gene="iscX"
                     /locus_tag="WGO65_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ0326662.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(3154..3555)
                     /locus_tag="WGO65_000310"
     CDS             complement(3154..3555)
                     /locus_tag="WGO65_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0326663.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            3766..4725
                     /locus_tag="WGO65_000311"
     CDS             3766..4725
                     /locus_tag="WGO65_000311"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ0326664.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            4793..6103
                     /gene="tilS"
                     /locus_tag="WGO65_000312"
     CDS             4793..6103
                     /gene="tilS"
                     /locus_tag="WGO65_000312"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ0326665.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            6177..6962
                     /locus_tag="WGO65_000313"
     CDS             6177..6962
                     /locus_tag="WGO65_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0326666.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEGKMPSEEVRRLRAVVPEGKVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGSADVWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLASYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            6972..7169
                     /locus_tag="WGO65_000314"
     CDS             6972..7169
                     /locus_tag="WGO65_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326667.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLFWMWWKWF"
     gene            7431..7784
                     /locus_tag="WGO65_000315"
     CDS             7431..7784
                     /locus_tag="WGO65_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326668.1"
                     /translation="MKNCKFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            7796..8101
                     /locus_tag="WGO65_000316"
     CDS             7796..8101
                     /locus_tag="WGO65_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326669.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDLVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            8163..9539
                     /gene="mpl"
                     /locus_tag="WGO65_000317"
     CDS             8163..9539
                     /gene="mpl"
                     /locus_tag="WGO65_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ0326670.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVYEGFDTAQLDEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWDVAEALAPLGGRLHVGKDFDAFVAEIVKNAEVGDHILVMSNGGFGGIH
                     GKLLEALR"
     gene            9746..10798
                     /gene="bioB"
                     /locus_tag="WGO65_000318"
     CDS             9746..10798
                     /gene="bioB"
                     /locus_tag="WGO65_000318"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ0326671.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(10876..11511)
                     /locus_tag="WGO65_000319"
     CDS             complement(10876..11511)
                     /locus_tag="WGO65_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0326672.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGSHVTRAVELYRDRFISYSGGNFATYGAIKTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQIGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            11834..12094
                     /locus_tag="WGO65_000320"
     CDS             11834..12094
                     /locus_tag="WGO65_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0326673.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(12157..14016)
                     /gene="ilvD"
                     /locus_tag="WGO65_000321"
     CDS             complement(12157..14016)
                     /gene="ilvD"
                     /locus_tag="WGO65_000321"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ0326674.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVEHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(14189..15958)
                     /gene="cysI"
                     /locus_tag="WGO65_000322"
     CDS             complement(14189..15958)
                     /gene="cysI"
                     /locus_tag="WGO65_000322"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ0326675.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEEFARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(15985..17799)
                     /locus_tag="WGO65_000323"
     CDS             complement(15985..17799)
                     /locus_tag="WGO65_000323"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ0326676.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAAAANAWTDNIAALLKEEAAKNRATP
                     APQTTPPAGLQTASEGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(17796..19082)
                     /locus_tag="WGO65_000324"
     CDS             complement(17796..19082)
                     /locus_tag="WGO65_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ0326677.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(19121..20044)
                     /gene="cysD"
                     /locus_tag="WGO65_000325"
     CDS             complement(19121..20044)
                     /gene="cysD"
                     /locus_tag="WGO65_000325"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ0326678.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(20082..20822)
                     /locus_tag="WGO65_000326"
     CDS             complement(20082..20822)
                     /locus_tag="WGO65_000326"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ0326679.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIAGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(20833..22275)
                     /gene="cobA"
                     /locus_tag="WGO65_000327"
     CDS             complement(20833..22275)
                     /gene="cobA"
                     /locus_tag="WGO65_000327"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0326680.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGSVAARKISLLLDAGAQVRVVANQ
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFQLAESCQKPVNV
                     VDDRDYCSFIFPSIINRNPIQIAVSSSGSAPVLARLLREKLEALLPHSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGDRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAENAPKPALIVIGEVVGLHEKL
                     AWFGENGEGENRVGQAYPALGGLNAGQWAA"
BASE COUNT         4861 a         5823 c         6445 g         5320 t
ORIGIN      
        1 tatataaaat cggcatttcg gatgtaagca attttgaagg cagaatgcgc catttggaaa
       61 acaacggcta tgcgaacgtt gccggattgg aacgcatcct cgccgtcaaa accgacaatt
      121 acaaagaaaa agaaaacctg ctccatgaaa ttttcagcaa aagcaggata ggcgataccg
      181 aattgttcgc cgtggacgaa aaccttgtaa aacgtttgtt tttatcgctt cgcggcgaaa
      241 tcgtgtttcc gaaaaacgaa acggcggaat cggaatttga aaaaagcgtc cacgaacgca
      301 ggcaggaagg gaatgccggg tccggccgca aacaactgct tgatttggta cggcgcggac
      361 accgggaata cccttacgcg ctgccccggc ttttggcggg cgcggcatcc tacaagccga
      421 aaaaatcgaa aatccgcctt tttaaagaag catatttcgg caaaagcggc acgaggctga
      481 ccgacgaaat tgcagacggc atccatattt acacctgttt ttcgcgggcg gatttggaaa
      541 aagcctattc cgaatatttg gaacttttca aatccgaatc ggatgccgaa ggcagaaagc
      601 cgcagtaagg tgcaaacaga taccgtacac gttgaggagc agatatgatg ggcgattccg
      661 tcatttatta tgtagaacag gcagacaaac cggtaaaccg tgccggcgaa cgcgcccgta
      721 aaacattcaa atatttttgg cgcgagcttt tttgggaacg ccgccgcatc attcccgcct
      781 tagattttgc catggtcaaa gccccttttt tccaagacgg cgaagacggc gaaatttgcg
      841 aacatatgtg gatcgacgat atttattttg acggtcttta catttatggc gtgctgaaca
      901 atgaacccgg cgaactgacc aatgtcgaac aaggcgaaag cgtttgcgtt ccggttgacg
      961 acatcagcga ctggatgttc gtgtgcaacg gcattcccta cggcggcttt accatacagg
     1021 caatgcgtgg acagatgacg gaagaggagc gtaccgaaca cgatgccgca tggggaatcg
     1081 atttcggcga tcccgggcag gtattgctgg tgtatgaaga aaaagaacat cccgaaaatc
     1141 tggaagagca tccgatgtgc cggaactgta ttgacgattt tcggcaacag ttgtcccaaa
     1201 atccggattt tctgcatgag caggacgaag acggctatac gccgcttcat catgaagcca
     1261 tcgcaggaaa tgcacttatg gttcaagcca tgcttgaata cggcgcaaat cctgcctcaa
     1321 agacatcgga aggctatacc gccctcgatt ttgcccgcct ggcgggctgg caaaatgttg
     1381 ccgacctgct cgaaccgcga cattaggcgg acagttttcc gaaaacgaac acaaacactt
     1441 tttacagaaa gacaataaaa atgcccaaaa tcaccgtact tccacacacg acattatgcc
     1501 ccgaaggcgc ggttatcgaa gacgcgcccg aaggcaaaac cgtccttgac gtgctgctcg
     1561 accatgatat cgaagtcgat cacgcctgcg aaaaatcctg cgcctgcaca acctgccacg
     1621 tgattatccg caaaggtttc gacagcctgg aagagccgac cgaattggaa gaagacctgc
     1681 tcgatcaggc ttggggtttg gaagccgatt cgcgcctgag ttgtcaggcg gttgtcgccg
     1741 gcgaggattt gattgtggaa atccccaaat acaccatcaa ccacgcgcga gaagaacact
     1801 gaaaacaggc cgtctgaagc cggcacgctt cagacggcat tgttgcgcgg ataaggcgca
     1861 atcgcccgaa aacaggcgtt cgtacaggcg gaactttcga ttctatagtg aattaacaaa
     1921 aatcaggaca aggcggcgag ccgcagacag taccaatagt acggcaaggc gaggcaacgc
     1981 tgtaccggtt taaatttaat tcactatata ttgattttta tcggttttct gacggaacgg
     2041 attggtgcgg catccgaggc ggattactcg ggcgcgatgc acgggtattt atcggttttg
     2101 cagccggaaa aaccgccggc gggttacttt cagggcggcg gtcgggcggt atgccggata
     2161 gcgttccact ttcgcttcta tattgatttt gattggtttt ctgacggaat gatccgatgc
     2221 ggcatccggg acggcttgtg tttcttcctg cccgcctgcc ggatattcct gtacttgcac
     2281 gaaaccgaaa gaggtggcgg tgcggcggac aagctcgaga tagcgtcctt caagctccgg
     2341 acaggcggcg gacacgcttt ctgccgtaac cgtgaagccg ccgcccgacc cggcaaggcg
     2401 ttccgcaatc tgcgtgtaga tgcgccaacg ttgggcgcgc aacattgccg catcgcaacc
     2461 ggcagccgct ttatccaagg caatcagacc gcgcctgctc cggtagtgaa ggcaaaccgt
     2521 tactctggag aggatatgtt gcccgtccaa tatttgatac agtttgcgta tcagcagaat
     2581 caggcggtca aactcctcca tctccgacag ggaatcagga tggtcggaag cggtataaac
     2641 cagtttggac aattttgcgg caaacatatt gttcatcaat cttccttgtc ggttgaaacc
     2701 ccgctcttcg gggcggtaga atcagatttg tttgggaggg acaactcctc ccagatcagg
     2761 acgacacata ggctggtgct tgatgtgttg tccggcgagt tgaaacattc agccatcctc
     2821 aaggtgcggc agttttgccg aaacatattc tacacggctt caatgccgga cgataaaagg
     2881 aaattcatat gaaatggacc gacacccggc gtatcgccga agaactctat gacctgcacg
     2941 gcgaaaccat cgatcccaga accgtgcgct ttacccaact gcgcgacctg attatggcat
     3001 tgcccgaatt tgacgacgac cccgcccgtt gcggcgaacg catcctcgaa gccgtgcagc
     3061 aggcatggat agacgaggcg gaataagttt cgggaatgcc gtctgaaatg cggcggtacg
     3121 cggttcgtgc ttctgtttgc agcgggaatg gttttaccag tctccttttt tcagcctgtc
     3181 cagttggcgg cggtcgcgct tggtcggtct gccgtcggga taggcggaag tgatgcggct
     3241 gaattggtcg agctgtttgc gctcttccct caatgttgcc gttttcgcgt cctcttcata
     3301 cagaagccgc gcctcggatg ccgggcggcg ttggtggttc aaacctttaa ccttgatttt
     3361 atagggaagg gaattgagcg tcaggtcgat aatatcgccg atgtctatgg ttttactgtt
     3421 tttgaccttc gagccgttta cttgaaccct acccagttcg atgtgctttt gcgcaaggga
     3481 acgggtcttg aaaaaacgtg ccgcccaaag ccatttgtcc agccgcatgg cggaagaatc
     3541 gtgcttgtct ttcatacgat tttgtttgaa ataattgaat ttgtttcaag tttagcataa
     3601 gatacgccgc ctataactag tatatatgca ctaatccact gttttccatg ctgtccgaac
     3661 acaaaaagag ggtgtggaaa agccgttttg gacaataaat taactgcgga atatgcacaa
     3721 atagcgtatg atagcggcag aatctgttga tgagagcttc attctatgaa acctgttttt
     3781 ttggattttg aacaacccat agccgaactg accaacaaaa tcgatgagct gcgtttcgtc
     3841 caagacgagt ctgccgtcga tatttcggac gaaatacacc gtttgcagaa aaaaagcaac
     3901 gacctgacca aatcgatttt cagcaaactt acgcccgccc aggtttcaca ggtttcccgg
     3961 catccgcagc gtccctatac tttggattac attgaggcac tgtttactga ttttgaagag
     4021 ctgcacggcg accgccactt tgccgacgat catgcgattg tcggcggatt ggcgcgtttc
     4081 aacggacaaa gcgtgatggt cgtcgggcat cagaaagggc gcgacaccaa agaaaaaatc
     4141 cgccgcaact tcggtatgcc gcgccccgag ggctaccgca aagccctgcg cctgatgaaa
     4201 acggcagaaa aattcggctt gcccgtcatg acctttatcg atacaccggg cgcgtatccc
     4261 ggcatcggcg cggaagaacg cgggcagtcg gaagccatcg gcaaaaacct gtacgaactg
     4321 acacgcctgc gcgttcctgt tttgtgtacc gtcatcggcg aaggcggttc gggcggtgcg
     4381 ttggcggtcg ccgtgggcga ttacgtcaat atgctgcaat actcgaccta ttccgttatt
     4441 tcccccgaag gctgcgcttc gattttgtgg aaaaccgccg aaaaggcggc ggatgcggct
     4501 caggctttgg gcattactgc tgaccgcctg caaaagctgg acttggtcga taccgtcatc
     4561 aaagaaccat tgggcggcgc gcatcgggat tttgggcaaa ccatgaaaaa cgtgaaagcc
     4621 gttttggaaa aacaactgca cgaagcgcaa agcatcccgc ttgccgattt gctttcgcgc
     4681 cgtttcgacc gcattatggc ttacggcaaa ttttcggaac aataattcag gtagaacaag
     4741 cagcaagcag tttgtctgaa actgcttgct ttttctttat cgggacggaa ccgtgctgac
     4801 tttagatgcg tttgagcaat gcttgaagga ttgttttcct caaggtctga atggaaaaaa
     4861 aacagcggtg gcattaagcg gcggcttgga ttccgtcgtt ttgctgcatc tgcttgtccg
     4921 cgccggaaaa aagggcggtt ttattccgga tgcattgcat atccatcacg gcttgagtcc
     4981 ccgtgccgac gattgggcag atttctgcca aaactattgc gatatgctcg gggtggggct
     5041 ggaaacggtt aaggtctgcg tggaaaaaaa cggtttgggc atcgaggcgg cggcgcggca
     5101 aaagcgttat gccgcgtttg ccgaaaaagg ctttgacgtt ttggcgttgg cgcaccacag
     5161 ggacgatcaa atcgaaacct ttatgctggc agtcgctcgc ggcggcggtt tgcgcgcttt
     5221 ggcggctatg cccaccgtcc gcccttttgg ggaaaaaggc atcatctggc ggcctttgct
     5281 gcctttttcg cgccaagaca tatgggatta tgcccaaaaa cacggtttgc cgaatatcga
     5341 ggacgaaagc aatagcgata cggcttattt gcgaaaccgc ttccggcacc gtattttgcc
     5401 cgaactttcg gcgcagattc cccatttcgg gcggcatgta ctgaacaatg tccgcgcttt
     5461 gcaggaagat ttggctttgt tggacgaaat catcggtcag gactgccgtt gggtctgtga
     5521 ggaaggcagt ttcgatacgg tgcgttggca ggcgttttcc ccacgccgga aaacccatat
     5581 tttgcggtat tttttgaagg aaaacggcat ccccatgccg aatcagaatg cccttgccga
     5641 cattgcccgg gttttgacgg aggcaaaaac cggacgttgg aacttgcaag gctttgaatt
     5701 gcatcattat gcaggcaggc tgtttgtgtt ccgactggaa aaaacggata aactgcggtt
     5761 tttgaaagac aggcagataa gcggaaattt aagggaaata ttgacggggc agggatttgt
     5821 tttaaaacgt catccgtttg ggcttcctga gcatcttttg gagcaggacg gaattttgag
     5881 gacggtagcg gtatcggata cgttggctgt cggcggcatc cataaggatg tgaaaaaaat
     5941 ccttcagggg aaacgggttt tgcctgtcct gcgcccaatt tggccgcttg ttgccgacag
     6001 cggaaaccgt ccattggcgt tggcaaactg ttgtgcggat ttccaatact cggtttcaga
     6061 cggcattttg cccgtccatc ctgactttcc cattttattt tgataatatc gcaaacagat
     6121 ttcggcggcg ttcagacggg tattgtccgg ttgcatattt ctaaaaggct tgtgaagtga
     6181 aacacatcag ttcgaccaat aatgaacaca tccggcacct gcaccgcctg ttgtcgcaag
     6241 gaaagttcag acggcaatac gcccaaaccg ttttggaggg cgtgcacctg cttcaggttt
     6301 tcctgcaatc cggcagaaaa ccggtcgggg tatatattcc cgagggcaaa atgccgtctg
     6361 aagaagtccg caggctgagg gcggttgtgc cggaaggcaa ggttttttcc gtttcagacg
     6421 gcatattgaa aaaaatcagc agcttgactt gtgcggatga tgtgcttgcg ctgattgata
     6481 ttccggatgc gggtgctttg cctgccggcg gcgactgcgt ggttttggac ggcgtgcaag
     6541 acccgggcaa tgtcggcacg gtgttgcgga gcgcggcggc ggcaggggta ggtacggtcg
     6601 ttttgggcag gggttcagcg gatgtttggt cgcccaaggt attgcgcgcg gggatgggcg
     6661 cgcatttctt gttggacatt tattcgcagg cggatttgga aatatggttg gcgagctata
     6721 aaggccgtgt gtttgccacc gccttgcgag aggaaaagca ggcggttttg tacggcgaag
     6781 atttgtgcga accgacagcc tgggtgtttg gcaacgaagg cgcgggggtc ggtaaagcgg
     6841 ttttggacag ggcggacaag tgtgtcagga ttccgatgca cgatgcaacc gagtctttaa
     6901 atgtcgcgat ggcggcgaca atctgcctgt ttgaacagat gcggcagcgg tcggcgtatt
     6961 gaggaaaaga aatgccgtct gaaaaaatct attacggcgt attgattttc ttatgtatcg
     7021 cttctatgct gctgtcgccg tttttttatg cgggtgcttt gaagcccaag aaggcggcat
     7081 tgcggaagga cgggcagtgg aaactcatct tattgtccaa taccgtggcg gcggcggttt
     7141 tgttttggat gtggtggaaa tggttttgac agatattgct caaaattggg ttaatggaat
     7201 ccgaacaaat aaataatttg gtaaaaaatt tgttaaatca atggattaaa gtttttcggg
     7261 aaacaaaaca gctctaagca aatagggcgt ttgtcggtaa atacggaaga gttgcggcat
     7321 tatcgggcat ctttaacaag tagtgccggc ttggcaggca attggttttt atgggcagct
     7381 tgcaaaatcg cggatataaa attgcgaatc ggttaaagcg tgaggatacc atgaaaaatt
     7441 gcaaattttt ttatgatccg acaagggcaa tctatgatag cggtgcagat tatttaacca
     7501 gagaaaaaca tagattagtc gtaattgcaa atagtgcttg ggggctattg cttaatttat
     7561 cttgttatta tgacgaggtt ttggaaaagc ggaaaatacc gttcggcaaa caggaaattg
     7621 atgacgatat ggacaaagtg tccgccctta agcggaagtt taaagatatt tctgaaatca
     7681 aagtagggga tggttgggaa tacccgttca attatgagca gggaatgaaa gaattagatg
     7741 aagtgctatt gaaatacatt cccttttttg aagaagaacg ataaaggagg ttgatatgcg
     7801 cgtatctaaa ataattggaa gtatgttgct tgttacagcg gttcagaccg tattttcggc
     7861 aaatgtttac gcgtgccgcc ataatggtaa aaccagttac agccaaactc cgggaaaaca
     7921 ttgtaccaac gcgggtttgg ggcgggacct ggtgtacagt tcggttagac cggcagtaaa
     7981 agacagggcg gaagacgcag gggtcggcga ttattcggac acggtgaggg acgaacacgt
     8041 ccaaaatccg agagagaata cgcataagga tgcttcgaat accggtgcaa agacgcattg
     8101 attgaaaccg aatcagccat gacggcgttg aataatagaa tttgaacgat tagggaactc
     8161 tgatgaaaca catccacatt atcggtatcg gcggcacgtt tatgggtggg attgccgcca
     8221 ttgccaaaga agcagggttt gaagtcagcg gttgcgatgc gaagatgtat ccgccgatga
     8281 gcacccagct cgaagccttg ggcataggcg tgtatgaagg cttcgacacc gcgcagttgg
     8341 acgaatttaa agccgacgtt tacgttatcg gcaatgtcgc caagcgcggg atggatgtgg
     8401 ttgaagcgat tttgaaccgt gggctgcctt atatttccgg cccgcaatgg ctggctgaaa
     8461 acgtgctgca ccatcattgg gtactcggcg tggcggggac gcacggcaaa acgaccaccg
     8521 cgtctatgct cgcgtgggtt ttggaatatg ccggactcgc accgggcttc cttatcggcg
     8581 gcgtaccgga aaacttcagc gtttccgccc gcctgccgca aacgccgcgc caagacccga
     8641 acagccaatc gccgtttttc gtcattgaag ccgacgaata cgacaccgcg tttttcgaca
     8701 aacgctccaa attcgtgcat taccgtccgc gtaccgccgt gttgaacaat ctggaattcg
     8761 accacgccga catcttcgcc gatttgggcg cgatacagac ccagttccac cacctcgtgc
     8821 gtaccgtgcc gtctgaaggc ctcatcgtct gcaacggacg gcagcaaagc ctgcaagaca
     8881 ctttggacaa aggctgctgg acgccggtgg aaaaattcgg cacggaacac ggctggcagg
     8941 ccggcgaagc caatgccgat ggctcgttcg acgtgttgct tgacggcaaa aaagccggac
     9001 acgtcgcttg gagtttgatg ggcggacaca accgcatgaa cgcgctcgcc gtcatcgccg
     9061 ccgcgcgtca tgccggagtc gacattcaga cggcctgcga agccttgagc acgtttaaaa
     9121 acgtcaaacg ccgcatggaa atcaaaggca cggcaaacgg catcaccgtt tacgacgact
     9181 tcgcccacca cccgaccgcc atcgaaacca cgattcaagg tttgcgccaa cgcgtcggcg
     9241 gcgcgcgcat cctcgccgtc ctcgaaccgc gttccaacac gatgaagctg ggcacgatga
     9301 agtccgccct gcctgtaagc ctcaaagaag ccgaccaagt gttctgctac gccggcggcg
     9361 tggactggga cgtcgccgaa gccctcgcgc ctttgggcgg caggctgcac gtcggcaaag
     9421 acttcgatgc cttcgttgcc gaaatcgtga aaaacgccga agtaggcgac catattttgg
     9481 tgatgagcaa cggcggtttc ggcggaatac acggaaagct gctggaagct ttgagatagc
     9541 ccgggcgatg ccgtctgaaa gcccttcaga cggcatcgcc cggctgcgcg gcacaaaggc
     9601 ggaaaaaccg tttgccccgt attttcaaac gcgttacact tgccgccgct gttttcagcc
     9661 atttgattac ccgcaaccgc cgtcattgcg ccggcggttt gcctgtcagc gtcattgcgc
     9721 cgctgtaaat acgaaagaac acattatgac cgtatccccc gtcgccttgc gccgtaagac
     9781 cgagtgcaag cctcatccca ccgcgcgcta ttggaaaaaa tgcgatgtcg aagccctgtt
     9841 cggacttccc ttcctcgacc tcatttacca agccgccgaa atccaccgcc aaaatttcaa
     9901 cccgcgcgaa atccagcttt ccacgctgtt gtccatcaaa accggcggtt gtcccgaaga
     9961 ctgcgcctat tgtccgcaat cggcgcacca caataccaat ctgggcaaag agcagatgat
    10021 ggatgtggat gaaatcgtcg aaaaagccaa aatcgccaaa tcgcgcggcg caagccggtt
    10081 ttgtatgggc gcggcgtggc gcggccctaa acccaaagac gtggagacgg tttccgcaat
    10141 catcaaagcc gtcaagggct tgggtatgga aacctgcggc acgttcggta tgctcgaaga
    10201 aggtatggcg gaagacttga aagaggcggg cttggattat tacaaccaca acctcgacac
    10261 cgaccccgac cgctacaacg acatcatcca cacccgccaa cacgaagacc gaatggacac
    10321 cttgggcaaa gtccgcaacg ccggtttgaa agtctgctgc ggcggcatcg tcgggatgaa
    10381 cgaaacccgc gccgaacgtg ccgggctgat tgccagtctc gccaatctcg acccgcagcc
    10441 cgaaagcgtg ccgattaacc ggttggtcaa agtggaaggc acgccgcttg ccgatgccga
    10501 agatttggac tggacggaat ttgtccgcac catcgccgtg gcgcggatta cgatgccgca
    10561 aagttatgtc cggctgtcgg cagggcgcag caatatgccg gaagcaatgc aggcgatgtg
    10621 ctttatggcg ggcgcgaact cgatttttta cggcgacaag ctgctgacca cgggcaatcc
    10681 tgatgaggac ggcgacagaa tcctgatgga aaagctcaac ctgtatccct tgcagtttga
    10741 accggaaggc gaggtcgccg aagtggaaaa agcctctggg attaaagtgg attattgacg
    10801 attgaaaaat gccgtctgaa acccggaaaa aggctttcag acggcatttg tccggacggc
    10861 atttccaata tctttttacc ggcgcgtgat gctgccgtcg ggcgagacat ccagcccgtt
    10921 ccccttgggg aaatcgctgc ggttcagata aataatccgc ccgataacgg ttttctcggg
    10981 gtcgattttg gggattttat cgccgatttg agtgatgggg ataatgttgc cggaaacgaa
    11041 ccgcccctgt ttgtcggtga taattttgaa aatcggcgca atgccgctga tgccggaggt
    11101 tttgattgcg ccgtaggtgg caaagttgcc gccgctgtag gagatgaagc ggtcgcggta
    11161 aagttcgacg gcgcgagtaa cgtgcgaccc ctgcccgaat acgacatccg cgccggaatc
    11221 gacggcaagc cgtgcaaact cgacgacgtt gcccctgttt tccccataga agatttcggt
    11281 atcgaacggc aggtgttccg cctgtttccc ttccgcgccg ccgtggaaca tcacaatgac
    11341 gatgtcggct ttctgtttgg ttttggtaat ccgttttcta actttggcat aatcgttcag
    11401 tttgacggcg gcaaggttgg gggcgaagga gacgaagccg gatcttacgc cgtttttctt
    11461 caggatggcg gtttcaaacc tgttttcgat gcccgaatat ttgatgttca attcgtcaag
    11521 gttcgccctt tatgccgttt ccgcaccgca aaccgccggg gtcaagccct cggcgcatcc
    11581 tgccggaacg gaatccccgt actgccgatt gacggttcaa accccgcgcc cgtttcaaat
    11641 accggcgatg tggacggaca ggatgcgcct gacgaaaaga cagccgatac cgtttccatt
    11701 atcggcgtgg gcgacattat gctcggcagc aattatccgg tcgattacct gcccgatacc
    11761 aatattctga aaaacgtcga atctgccttg caagacgcgg acattaccgt cggcaacctc
    11821 gaaggcacgc tgtttgacga aggcggtacg ccgaaaaaat gtgcaacccc ccaaaatatg
    11881 ctatgcattc cgaacgccct ccgcatacgg gcaatacctt gccgacgcgg gattcgacta
    11941 cctcagcttc gccaacaacc acagcaacga cttcggcgcg caaggcatca cggcaacggc
    12001 ggcgagcggc agctctttta catactcgat cgcgctaaag ccgctgccga taacgaggcc
    12061 aaaattgcgg aaaataccgc catcgcccag ataaatttgt ccatcatcag acctttactg
    12121 ttcagacgag acagcatttg ccgcacgttt tggggcttat ctttcgattt gcgctacgtc
    12181 gcgcaccgcg cctttgtcgg cggaagtcgc catcgcgccg taagctctta atgcggcgga
    12241 gacgtagcgg tcgcggtttt taggcttcca tgctttgctg ccgcgcgctt ccatttcggc
    12301 acggcgtgcg gcaagctctt catcggaaat ggcaaggtgg atgctgcggt tggggatgtc
    12361 gatttcgacg gtatcgcctt cgtgtaccaa accgatcgcg ccaccttccg ccgcttcggg
    12421 cgaggcgtgt ccgatggaca aacctgatgt gccgccggag aagcgtccgt cggttaagag
    12481 agcgcaggct ttgccgaggc ctttagattt caggtaggaa gtcggataca gcatttcctg
    12541 cataccgggg ccgcctttcg ggccttcgta gcggatgatg acgatgtcgc cggcgacgat
    12601 ttggttgccc aaaatgcctt ctactgcgtc ttcttggctt tcaaacacgc gggcgcggcc
    12661 ggtgaatttg aggatgctct cgtccacgcc tgcggttttc actacgcaac cgcgctcggc
    12721 gatgttgccg aacaagaccg ccaaaccgcc gtcttgcgag taggcgtgtt cgacatcgcg
    12781 gatacagcct ttttcgcggt cgaggtcgag ggttttccac atgcggtttt gcgagaacgc
    12841 ttgggtggtg cgcacgccgc cgggcgcggc tttgaagcgt tcgatggcgc gggtgttttc
    12901 agggttggtt acgtcccatt tttcaatcgc gtctttcagc gtcggtgcgt ggatagtgtg
    12961 cacgtcggtg tgcagcttgc ccgctttgtc caattctttc aggatggcga agatgccgcc
    13021 ggctcggtgc acgtcttcca tgtagtagtc gtggttgttg ggtgcggttt tgcagatgca
    13081 gggcacaacg cggcttaagc ggtcgatgtc tgccattttg aaatccacgc ccgcttcgtt
    13141 ggcaacggcg agtaaatgca aaatagtgtt ggtgctgccg cccatcgcaa tatccatcgt
    13201 catagcgttt tcaaacgctt ttttggtggc aatgctgcgc ggtaacacgg tttcatcgtt
    13261 ttgctcgtaa tagcgtttgg tgatttcgac aatcatacgg ccggcttcga ggaacaattc
    13321 tttgcggccg gcgtgggtcg ccaaatacga accgttgccg ggtagggaca gaccgagtgc
    13381 ttcggtcagg cagttcatcg agtttgccgt aaacataccc gaacacgagc cgcaggtcgg
    13441 gcaggcgttt tgttcgactt cctcgacttg ccggttgctg acattgtcgt ccgccgattc
    13501 aatcatcgcg tcaatcaagt ccaagcggcg ttctggttgg atgtttgcca cgccgataac
    13561 tttacctgct tccatcgggc cgccggagac gaagatggtg gggatgttca ggcgcatcgc
    13621 ggcaatcagc atgcccgggg tgattttgtc gcagttggaa atgcacacca gcgcgtcggc
    13681 gcagtgggcg ttgaccatat attcgataga gtcggcaatc aaatcgcggc tgggcaggga
    13741 atacagcata ccgctgtgtc ccatagcgat gccgtcgtcg atggcgatgg tgttgaattc
    13801 tttggcgatg gcgccggctt tttcgatttc gcgggcaacc agctgaccca tattgtgcag
    13861 gtggacatga cctggcacga attgggtgaa ggagttggca acggcgatga tgggcttgcc
    13921 gaagtcggtt tccatcacgc cggtggcgcg ccacaatgca cgtgcgcccg ccatattgcg
    13981 gccgtgggtg gaggttttgg agcggtattc aggcatagtg tgtttccttg tgcctatacc
    14041 gtctgaaaga cagggctgtt tcagacggta tcgggtacgg ttttttagag tgggaaaaga
    14101 gggtatttta taccaagtat cggaattttg cgggattgaa acggcgtgcg gcaaaaaaga
    14161 aaatccccgc aggaatgcgg ggacgggttc aggcgcgggc aatcgcgacg gctttggatg
    14221 cgtcccaaaa atcaacgggt gcatttaata cgggtttgac gatgcccgtc cgtatggcga
    14281 aatcgccgaa accttcgccg atattgcgtt ctgccgccca tttgccgatc aggtcgtcca
    14341 attcggcaag gatttcgggc agggtgatgt tttctttgta aagacggggg atgcgtacgc
    14401 cttcgcggtc gccgccgatg tggaggttgt agcgtccgac ggctttgccg accagtccga
    14461 tttccgccaa catcgcccgt ccgcagccgt tcgggcagcc ggtaatgcgg gtaacgatgt
    14521 agtcgtccga cgtgccgtgt ttcgccataa tcttatccag ctcgccgatg aagtccggca
    14581 gcacgcgttc ggcttccgcc attgccagcg ggcaggtcgg aaaggaaacg caggacatcg
    14641 cattttcacg cagcttgctg acatcgttgc ggattaatcc gtatgttcgg gcaaattctt
    14701 cgatttttgc tttgtctgct tcggcgacat ttgccacgat gaggttttgg ttggcggtga
    14761 tgcggaaatc gcctttgtgg attttggcga tttccaacac gccggtcaga agctgtttcc
    14821 cgccttcgtc aaccaaacgc ccgctttcga tgaaaagggt taaatgccag ttgccgtcta
    14881 tgcctttcac ccagccgatg cggtcgccgc gcccggtaaa tttgaacggg cgtacgggtt
    14941 cgaacggcat acccatacgg cgttcgactt ccgcgcggaa gttgtccaag cccatatttt
    15001 gaatggtgta gcgggtgcgg gcgtttttgc ggtcgctgcg gttgccgaag tcgcgctgcg
    15061 tggttaccac cgcttcggcg gccttcagcg cgtgttccgg aggcacgaaa cccagttcca
    15121 gtgaaatgtt cggataggtt ttggtgttgc cgtgttccat cgaaagcccg ccgcctgcca
    15181 aaacattgaa gccggcaagc tgtccgttac cgtctgaaac ggcgacgaaa tccaaatcgt
    15241 tgccgtagca gtccacatcg ttcaagggcg ggatgacgac tgcggttttg aattttcgcg
    15301 gcagataggt tttgcccaaa atcggctcgt cttcttgaag gaagtcgtcg gaactttgaa
    15361 cttttttgcc gtccacccac acatccagat aaccgcgcgt gcgcggcagc aggtgttcgg
    15421 aaatcttttt cgcgtattcg taagcctgcc ggtgcagttc ggactcgatc gggttggacg
    15481 tgcaaagcac gttgcggttc atatccgccg ccgtggcgat ggaatccaaa cccagtttgt
    15541 gcaagaggcg gtgcatcgtc tgcaacttgg ctttcggcac gccgtgaaat tggaaggttt
    15601 gccggttggt cagccggatg gagcggtaat gactgttttc ccgggcaaat ttgtccagtt
    15661 ctatccattg ggacggtttg atgatcccgc ccggcagccg gcagcgcaaa agcataaatt
    15721 tcaagggctc gagttttgcc tcggcgcgtt cggcgcggat gtcgcggtcg tcctgctcat
    15781 acataccgtg gaagcggatg agttggaagt tgtcgccttt gaagccgccc gtgagcgggt
    15841 ctttcaaatc gtccaaaatc gtgccgcgta aaaaattgct ttcggttttc agacgttcgt
    15901 tgtcggatag cggtttttct tgccacgcca aaccttttgt cttggtctgt acggtcattt
    15961 tgtgttcctc ccgattatat ttaatcaata aacatcacgc tgatagcgtt tttcttcgcg
    16021 cagcatatcc aaatattctt ctgcgccctc ttcgtccaaa tgtcctgccc cgataatcac
    16081 atccagcaag gcggcttcca cgtcttttgc catttttgcc gcatcgccgc acacatagat
    16141 atgcgcgcct tcctgcagcc attgccaaag tccttccgcc tgttcgcgga ttttgtcctg
    16201 cacatagatt ttttcttcct gatcgcggga ccaggcgaaa tcgtacctgt gcaggaaacc
    16261 gtctttggca aactgctgcc attcggtttg atagagaaaa tcacgggcaa aatgcggatt
    16321 gccgaaaatc agccagtttt tgccttccgc attttctgcg gcacgttgtt ggacgaaggc
    16381 gcggaacggt gcgacgccgg tgcccgagcc gatcatcaca atcggcttgc ggctgtcttc
    16441 gggcagcctg aagccgtcgt tgcgttccac aaacacgcgc accgtgccgt cctcttccag
    16501 ccggtcggca aggaaacccg atgcgccgcc cgccctggcg cgcccttcgt gttcaaaacg
    16561 caccgcgccg acggtcaggt gcacttcgtc ccccacttcc gcctgcgacg aggaaatcga
    16621 atacaggcgc ggcgcaagtg ggcgcagcag gccggcgaat tgttccgccg tcagttttgc
    16681 cgggaagcgg tgcagcacat cggcaatcgg cgtgctttgc acaaagcctt gcaaaacggc
    16741 gttgtcggca gcaatacggt cgagttcgtc atcatcggcg aacggggcat agcctttgac
    16801 aaaggcgggg gtgttttgcg tgagttcaaa atgggataac agtgcggagg caaccggcag
    16861 ggtttttccg cccgcctgta tttccgttgc cggatcgatg ccgagcaggt ctaggatttc
    16921 cctgaccagt gccggatcgt tgtcaaacca aacgccgagc gcgtcgcccg ggaggtagtg
    16981 caaatccgaa ccgctcaaat cgatttcgat gtggcgcacg tctttatcgg attggcgggc
    17041 ggtgattttc tgattggcca gcagggcggc gggaaagggg tctgccttgc agtacctgcc
    17101 ctccgatgcc gtctgaaggc cggcgggggg cgttgtctgc ggcgcgggcg ttgcccggtt
    17161 ttttgcggct tcttctttta agagtgcggc gatattgtct gtccaggcgt ttgcggcggc
    17221 ggcaaagtcc aaatccgcat caacgcgttc gagcaggcgt ttcgccccca attcttcaaa
    17281 acgtttgtcg aaatctttgc ccgcccggca gaaattcgga taggaactgt cgcccaaacc
    17341 cagtacggca aattggagtt tgtccaattt cggggctttt ttgccgttca gcagtttgtg
    17401 cagcacgacg gcttcttccg gcggttcgcc ttcgccttgg gtggacgtaa ccagcagcag
    17461 gcggcgttcg ccggcgatgt ttttcgcctt atagtctttc agttcggcgc gactgacttg
    17521 gatgccggcg gcttccaggc tgtccgccgc tttgtcggca acggatttcg cattgccggt
    17581 ttgcgaggcg gaaaggacgg ttacggaaaa aggttctgcc gtcggcaatg ccgtctgaag
    17641 cgcgggcagt cctgcagatg ccccgtttcc tgcttttgcc caagcgtagc cggacagcca
    17701 cgcccattgt gccgcgtcca gccccgacag gagctgcgtg atttcgggcg gcagaggcgg
    17761 taatggcgga tttgtgttct gcatatcgtg ttcactcata aaatcatacc tgccgcaaca
    17821 gtgccgtatg tcgcttcgtc tatcaggata aacgaaccgg cggcggtgtt ttccgcataa
    17881 ggcgttgccg taacgggttt ttgaaggttg atgcggactt tggcgatgtc gttcatcttc
    17941 aaggattccg cgccggcctc ttgttccagc gtgcggacat ccaaaacgct ttcaatttcc
    18001 ccgacttttg ccggcacggt ttgcgtgccg tgcttgagca ggtatttgcg cgcggtgttg
    18061 agcggacgtt cgtcaaacca gcaaagcgtg gcttccagat gtttttgcgg ggcgagcggg
    18121 gaatttttat cgacaaaaag gtcgccgcgc gaaacatcga tgtcgcggtc cagccggagg
    18181 gttgccgcct cgccggcaaa agcctgcgcc acttcccctt tcggcgtgat gatttcggac
    18241 acttcggcgg tcagcccgtt cggttcgatg cggacggttt gcccgacggc gaccgaaccg
    18301 cgttcgatgc gcccctgata gcctcggaaa tcatcggcct tgtcggcatc ttggcggacg
    18361 accagttgca cggggaaata aaaatcgtcg gcggtgcggc tgacttcgtc cgcccccggc
    18421 agggtttcca aaatggacaa taagggttcg cctttatacc aaggcatatt gccgccgggg
    18481 taaacaatgt tgtcgcccaa gagtgcggac accggtacga aatgcacgtc tttcaaaccg
    18541 agctgttcgg caagtcggcg gtatgcctcc acaatggcgt tgaatttgtc ttcgctgtaa
    18601 tccagcaggt ccattttgtt gaccgccacc acaatatgcg ggcagtcgag ttggcggagg
    18661 atggcggaat ggcgtttggt ctgcggcaga agctgcaagg gctgcgcgcc gaaatccagt
    18721 tgggatgcgt caaccagcac gactgccgcc gaagcggtgc ttgcgcccgt aaccatattg
    18781 cgcgtgtatt gttcgtgccc cggcgtgtcg gcgatgatga atttccgttt cgccgtggaa
    18841 aaatagcggt atgccacatc gatcgtaatg ccctgttcgc gttcggcttc cagtccgtcg
    18901 gtcaggatgg agaagtctat ggtttctttc aaacctttgc ttttgccgga ttccaaggtt
    18961 ttgatttggt cggacagcag ggctttgctg tcgtagagca gtcgtccgat tagggtgctt
    19021 ttgccgtcat cgacgctgcc ggcggtaatg aagcggagcg gggtttggtg ttgtgccgtc
    19081 atattttctt cctcatatct gcttaaaggg tttttgaaat ttagaaatag ccttcttttt
    19141 tgcgtttttc cattgccgcc tcgcttgcct gatcgtccag ccgggtcgcg ctgcgttcgg
    19201 aaatgtcggc aaccgctgtt tctctgataa tctccgtcgg cgtggacgcg gtgctttcta
    19261 ccgggcaggt gcagctgatg tcgccgacgg tgcggaagcg gacatcaagg atttcggagg
    19321 tttcagacgg cattttcggg gtgagcggcg ttacagggac cagcagcccc ctgcgtctga
    19381 ccacttcgcg cctgtggctg taataaatcg gcggcagctc gaggttttcg cgggcgatgt
    19441 attgccagat gtcgagttcc gtccagttgg aaatcgggaa gacgcgcata ttttcgcctt
    19501 tgtgcagcct ggtgttgtac agcgaccaca gctcggggcg ttgcgccttc ggatcccatt
    19561 gtccgaactc gtctcggaac gagaaaatcc gttctttggc gcgggctttt tcttcgtcgc
    19621 gccgcgcgcc gcccatcagc gcgtcaaagc cgtttgcctc gatggtttcc aacagcgtaa
    19681 ccgcctgcgc cgcattgcgc gaatcggttt ctttgcgtaa aactactgtg cctttggcaa
    19741 tggagtcttc cacgcgcccc actatcaggc gggcattgag ttttgccgcc tgcgcgtcgc
    19801 ggaaagcaat cacttcgggg tagttgtgcc ccgtgtcgat atgcaccagc gggaagggca
    19861 gttttaccgg cctgctgccc agccggaagg ctttgcaggc gagggcgagc aggaccacgg
    19921 aatctttgcc gccggaaaag agcagggcgg ggttttcgca ttccgccgcc acttcgcgga
    19981 tgatgtggat ggattcggat tccaaccagt cgagttgggc gttgttcggt tcggttttcg
    20041 tcataccata ttccttattt cttctgtctg atatttatga attatttgtg cagcccgcat
    20101 tctttgctgt ttctgccttc ccaccaccac cgcccggcgc ggatgtcttc gcccgccttg
    20161 acggggcggg tgcaggggtc gcagcctatg ctgggaaatc cttgccggta caaatcgttg
    20221 taaggcacat tgttggcgag gatgtatgcc cacacgtcgt gttccgacca gtcgaaaatc
    20281 gggttgtatt tgccgatgcc ccgtccggca tcgtattcgg caaacggcag ttccgtgcgt
    20341 gtggcggatt gttcgcggcg ttgcccggta agccaggcgt ccgcgcctgc aatggcgcgg
    20401 ttgagcggtt cgatttttcg gatgcggcag cattcgcggc gcgcttcaac gctgtcgtaa
    20461 aaggcaaacc tgcctttgct ttccacataa cggtcggcat cttctcgaac cggccggaaa
    20521 cgctttatcc gcaaatgggg atatgcgcgt ccgagcctgt ccagcaggtt cagggtttcc
    20581 gtgtggagca gacccgtatc caaggtaaaa atgccgatat tgaggttttc gccggcgata
    20641 aggtcggtaa tcaccatatc ttctgccgca aggctgctgg caaaccgtgc atcccggtgg
    20701 ctgccggcaa tccggtgcag gcgttgtttg agggtttccg ttttttccgc aagggcggtt
    20761 tcgccgccgg atccgatatg cggtatctgc cacagggcgg gtttgaacag tgtcgtttcc
    20821 atttttcccg ccttatgccg cccattgtcc ggcattcagt ccgcccaatg cgggatacgc
    20881 ctgcccgact cggttttctc cttcgccgtt ttcaccgaac caggcgagtt tttcgtgcag
    20941 ccccaccact tcgcctatga caatcaatgc cggcttcggc gcgttttcgg cgagttcggc
    21001 aaggttggcg agcgtgccgg ttgcggtttt ttgagccggc agcgtgcctt ggctgataac
    21061 ggctgccggc gtgtcgggcg agcgtccgtg ctgttgcagc cgttcggcaa tcagggcggc
    21121 tttgagcgca cccatataaa tcaccaaggt ctggcggctg cgggcgaggg tctgccattc
    21181 gatgtcgggc gcatccgcct tgcggtggcc ggttacgaaa accgcacttt gggcataatc
    21241 gcggtgcgtg agcgggatgc cggcataggc ggtcgcgccg acggcggcag taatgccggg
    21301 gacgaccgaa aacggaatct gatggcgtgc caaggtttcc aattcttcgc cgccgcgtcc
    21361 gaacacgaaa gggtctccgc ctttcaaacg caccacgcgc ctgccttcgc gggccagcct
    21421 gaccataagc gcattggtgt cctcttgcgg ggtgcggtcg ccccgggcgc gcttgccgac
    21481 aaaaatccgt tccgcatcgc ggcggacgag ggacagtatg ccgtctgaaa ccagcgcgtc
    21541 gtaaagcacc acgtctgcct gctggatttc ctgtagccct ttgagcgtca gcagccccgc
    21601 atcgccggga cccgcaccga ccaaagacac aaaaccgcct tgataatttt gttccaactg
    21661 ctttgccaac tcctgttcgg cttgggcggt ttgttgattt ttaacgaggg cggcaaaacg
    21721 gccgttgaac tgcttttccc aaaaacggcg gcgttcggta acggatttca gtttggcttt
    21781 gaccgcatcg cgccatcttc cggagatttc cgccatatcg cccaaagaat gaggcagcag
    21841 ggcttccagt ttttcacgca gcaaacgggc gaggacgggc gcgctgcccg aactggaaac
    21901 ggcaatttga atcggattgc gattgataat cgaaggaaaa ataaaactgc aataatcgcg
    21961 gtcgtccacc acgttgaccg gtttttggca gctttcggca agctggaaaa cgcgccggtt
    22021 gagggcttgg tcgctgcttg ccgcaatgat gaggaaaact gtgcggatgt gttcggcacg
    22081 aaattcttcg gcaagccaca ggattttgtt ttctgccgcc agcgcactta attcagcatt
    22141 caactgattt gcaacaaccc tgacttgcgc gccggcatcc agcaacaggc tgattttgcg
    22201 cgcggcgacc gaaccgccgc ctacgaccaa tacggggcgg ccggcgaggt tggcgaaaat
    22261 ggggaaataa ttcactggcg aactcctttg ctgtttgccc gcaccttgtt tccgatacgg
    22321 tgcgtcgcgg catttttgtc ggaatgcggg tcattttaga caaaaggatt ttcccggtta
    22381 aataataaaa aggcatttgt tagaagctga aagctatatg agggcggctg cggatgcggc
    22441 ggttttccg
//
LOCUS       DAWFAN010000012        22337 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWFAN010000012 DAWFAN010000000
VERSION     DAWFAN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.012"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(120..1034)
                     /locus_tag="WGO65_000328"
     CDS             complement(120..1034)
                     /locus_tag="WGO65_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0326681.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WGO65_000329"
     CDS             1290..2759
                     /locus_tag="WGO65_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ0326682.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3009..3380
                     /locus_tag="WGO65_000330"
     CDS             3009..3380
                     /locus_tag="WGO65_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326683.1"
                     /translation="MNKTLSILPAVILLGGCAVGGGNTFGSLDGGTGMGGSIVKMAVE
                     SQCRAELDRRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGSDVMQMLNPSTR
                     NQALAALTVKTVSACFKRLYR"
     gene            3453..4862
                     /locus_tag="WGO65_000331"
     CDS             3453..4862
                     /locus_tag="WGO65_000331"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ0326684.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGREALD
                     KWRGLAYPELAFEIMRLFVTDIPEGDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSEHTYVTSSPSMDISKASNFERFVFDLMDRNPL
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4908..5702
                     /locus_tag="WGO65_000332"
     CDS             4908..5702
                     /locus_tag="WGO65_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326685.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLAGIPLDAQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5890..6666
                     /locus_tag="WGO65_000333"
     CDS             5890..6666
                     /locus_tag="WGO65_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0326686.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6742..6848
                     /locus_tag="WGO65_000334"
                     /pseudo
     CDS             6742..6848
                     /locus_tag="WGO65_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7437..9908)
                     /locus_tag="WGO65_000335"
     CDS             complement(7437..9908)
                     /locus_tag="WGO65_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ0326687.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMERIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9905..11083)
                     /gene="hflX"
                     /locus_tag="WGO65_000336"
     CDS             complement(9905..11083)
                     /gene="hflX"
                     /locus_tag="WGO65_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ0326688.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11088..12368)
                     /locus_tag="WGO65_000337"
     CDS             complement(11088..12368)
                     /locus_tag="WGO65_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ0326689.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12521..13246)
                     /locus_tag="WGO65_000338"
     CDS             complement(12521..13246)
                     /locus_tag="WGO65_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ0326690.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGRRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13319..13996)
                     /gene="radC"
                     /locus_tag="WGO65_000339"
     CDS             complement(13319..13996)
                     /gene="radC"
                     /locus_tag="WGO65_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ0326691.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14119..15468)
                     /gene="gshA"
                     /locus_tag="WGO65_000340"
     CDS             complement(14119..15468)
                     /gene="gshA"
                     /locus_tag="WGO65_000340"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ0326692.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15728..17137
                     /gene="leuC"
                     /locus_tag="WGO65_000341"
     CDS             15728..17137
                     /gene="leuC"
                     /locus_tag="WGO65_000341"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ0326693.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17234..17488
                     /locus_tag="WGO65_000342"
     CDS             17234..17488
                     /locus_tag="WGO65_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326694.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17550..18191
                     /gene="leuD"
                     /locus_tag="WGO65_000343"
     CDS             17550..18191
                     /gene="leuD"
                     /locus_tag="WGO65_000343"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ0326695.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18360..19631
                     /locus_tag="WGO65_000344"
     CDS             18360..19631
                     /locus_tag="WGO65_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0326696.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19647..20378
                     /locus_tag="WGO65_000345"
     CDS             19647..20378
                     /locus_tag="WGO65_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0326697.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20412..21482
                     /gene="leuB"
                     /locus_tag="WGO65_000346"
     CDS             20412..21482
                     /gene="leuB"
                     /locus_tag="WGO65_000346"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ0326698.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            21645..22208
                     /locus_tag="WGO65_000347"
     CDS             21645..22208
                     /locus_tag="WGO65_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ0326699.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSATIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5393 a         5866 c         5813 g         5265 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggtggcg ggatttttta ttttcccgat tgaagtataa tgtcgccgga ttcaaccgga
     2881 tattcaaacg gtttactcca acactcggaa cggcgcataa aacgccgccc tgcgcgttat
     2941 cccgaacggg gcggctaatc agatcctatc gccataaacg gcggggggtc aaccgaaaag
     3001 gaattgagat gaataaaacc ttgtctattt tgccggcggt aatcttactc ggcggctgcg
     3061 ccgtcggcgg cggtaacaca ttcggcagct tggacggcgg cacgggtatg ggcggcagta
     3121 tcgtcaaaat ggcggtcgag agccaatgcc gcgcggaatt ggacaggcgc agcgaatggc
     3181 gtttgaccgc gctggcgatg agtgccgaaa aacaggcgga atgggaaaac aagatttgcg
     3241 cttgcgtcgc ccaagaagcc cccgaacgga tgaccggcag cgatgtgatg cagatgctga
     3301 atccgtccac gcgcaatcag gcacttgccg ccctgaccgt caaaacggtt tccgcctgct
     3361 tcaaacgcct ataccgctaa ccttcttcag acggcatgaa gcaaatgccg tctgaatccg
     3421 ttttcccaca atttcgacag acgagaaaaa tcatgaaata tatcagcacg cgcggcgaaa
     3481 ccgcacacaa accgttcagc gaagttttat tgatggggct tgcacccgac ggcggcctga
     3541 tgctgccgga acattatccg caaatcgggc gggaagcctt ggacaaatgg cgcggtttgg
     3601 cttatcccga attggcgttt gaaattatgc gcctgttcgt taccgacatt ccggagggcg
     3661 atttgcgcga cattctgaac cgtacttaca cggaagcggc gttcggtact aaggaaatca
     3721 cccccgtccg cacgctttca gacggcatca aaatccaagc cttgtccaac ggcccgacgc
     3781 tggcgttcaa agatatggcg atgcagtttt tgggcaatgc gtttgaatat gttttaaaca
     3841 aagaaggtaa aaaactcaat atcttgggcg caaccagcgg cgatacgggt tcggctgcgg
     3901 aatatgcctt gcgcggcaaa aaaggcgtga acgtatttat gttgtcgccc gacggtaaaa
     3961 tgagcgcgtt ccaacgcgcg cagatgtaca gcttgcaaga cgagaatatc cacaatatcg
     4021 ccgtgaaggg gatgtttgac gactgtcagg acattgtgaa ggcggtgcag aatgatgccg
     4081 cgttcaagga aaaataccat atcggtacgg tcaactcgat caactgggga cgcatcgtcg
     4141 cgcaagtggt ttattacttt gcaggctatt tcaaagcgac gcaaagcaat gacgagcaag
     4201 tcagcttctg cgtgccgagc ggcaacttcg gcaacgtttg cgcgggacac atcgccaaac
     4261 agatgggttt gcctatccgc cgcctgattg tcgcgaccaa tgaaaacgat gtgttggacg
     4321 agtttttcaa aaccggcgca taccgcccgc gcaacagcga gcatacttat gttacctcca
     4381 gcccgtcgat ggacatttcc aaagcgtcca acttcgagcg tttcgtgttt gacctgatgg
     4441 atcgtaatcc tctggaaatc aatacgctgt gggcggaagt ggcggcggga aaaggctttg
     4501 acctgcggtt tgctttagaa aaagtcggtg gcaaatacgg ttttacctcc ggcaaatcca
     4561 cccaccccga ccgcctcgcc accatcagac aggtttatga gcaagaccaa gaactcatcg
     4621 acccgcacac tgccgacggc gtaaaagtcg cccgcgaagt gcgcgaagaa ggggaaacgg
     4681 ttgtgtgttt ggaaaccgcg ttggcagcga aattcgatgc gaccatacgc gaagccgtcg
     4741 gcgatgccgc cattccgcgc cctgccgcgc tggaaggttt ggaaaacctg ccgcagcgcg
     4801 tgcaaaccgt gccgaacagt gcggatgcgg taaaaggcat catcgaacaa acccttgcct
     4861 gatattccgt acaggggcat tccgtccgaa cttaagattg aaagaaaatg accttaccta
     4921 tgcaggaaac ccgtttttct atactgctgg acgaactggc ggcaaaacag gaggcagcca
     4981 tcgctcccca cctgcttgcc gacggaacca aggtatgggt acgcaaagca ggcaggcaca
     5041 atgcgcggtg gcgttacgcg ctgctcggta tggttgcccg atatttgaaa ctgggggtgt
     5101 tgaagccggt tcccagcctc ggcggcgagc ctgccattgc aaccgaatca aaacgcctgt
     5161 acgaattgcg ctcggcgggg atagccgttc ccgaattgct cgccgtcagg aaaaatgccc
     5221 ttatgttcgg caatttggca ggcatcccgc tcgatgcgca aatccgccaa gaagccgaag
     5281 ccggaaaggc ggacgcttgg cttgccggtt tggaagccat tgcacgcgtg cataaaaaac
     5341 agcagttcct cagtcaggcg tttgcacgaa atatgatgtc ggacggaaaa aacatcagct
     5401 ttttggattt tgaagacgac ccttccgagg tcctgaccat agcgcaatgc caagcccgcg
     5461 attggctctg ctacattcat tcgaccgcgc tgatattgaa aaacggcgga ctgctggaag
     5521 cggctgcgga aaaatggggc ggcgtattgt cagatcagcc tgccgaaata caaaagctga
     5581 ttgccgccac cgtcaaaccg atcctcccga tacgcaggct ggaacacccc cgctggggac
     5641 gggacgcgct caggctggca gcctcgattt ccctgatttc ccttgccgat atgccgccgt
     5701 aagccgccgc ccgccccacg atgccgtctg aagcccttca gacggcatat aaccaacgcg
     5761 catcaagcat tgtcgcaacc atccgtttcg tttagaattt tggctttacc ccgaatcggg
     5821 cagacatttt cactctgccg cacatcccga acaagcacta tccatttctt ttactgacag
     5881 gaaacccaaa tggcagcatt caatacccaa aaagtattgt ccgtacacca ctggacagac
     5941 gcatatttta cctttacctg cacccgcgac gaatcgttgc gctttgaaaa cggacagttc
     6001 gttatggtcg gattgatggt ggacggcaag ccgctgatgc gcgcatacag cgtcgcctcc
     6061 gccaactggg aagaacacct cgaatttttc agcattaaag tccaagacgg tccgctgacc
     6121 agccgcctgc aacacctcaa agtcggcgac gacgtgttaa tcagcaaaaa accgaccgga
     6181 accctggttg ccggcgacct gaatccgggc aaacaccttt acttgttgag caccggtacc
     6241 ggcatcgctc ctttcttgag catcaccaaa gaccccgaaa tttacgagca atttgaaaaa
     6301 atcatcctcg tacacggcgt gcgctacaaa aaagatttgg cgtactacga ccgctttacc
     6361 aaagaattgc ccgaacacga atacctcggt gacttggtta aagaaaaact gatttactac
     6421 ccgattgttt cgcgcgaaga attcgaacac cacggtcgcc taaccgacct gatggtgagc
     6481 ggcaaactgt ttgaagacat cggcctgccc aaaatcaacc cgcaagacga ccgcgccatg
     6541 ctgtgcggca gccccgcgat gctgaaagat acctgcaaag ttttggacga tttcggactg
     6601 accgtctctc cgaaaacagg cgtacgcggc gattacctga ttgagcgtgc gtttgtagat
     6661 caataagctg tttgaaaatc ccgtctgaac ggcaccagca atcaaaaacc ccgaatcctg
     6721 ccaatcagga ttcggggttt ttatagtgaa ttaaatttaa atcaggacaa ggcggcgagc
     6781 cgcagacagt acaaatagta cggcaaggcg agccaacact gtaccggttt aaatttaatt
     6841 cactatatag tgggtaaaaa ttagaaatgc acatttccgt cattccaaca caggtgggaa
     6901 tccggactta cctgtttcat tatcgtttga aaataaaaga gaaaccaaac atatagattc
     6961 ccgcttgcgc gggaatgacg acggtgtttt gccgtagcca aagcagaaaa acatccctat
     7021 tgtcatttca tcatgatatg acaataggat gttaaaaatt aattatcatt atatgattga
     7081 tatataagaa aaactgtccc aataagaaat gttatgcaga aaataaaatg aaaaggatgg
     7141 ttttcattga aaatggttgt tctgccaccg tcatacacag agccagtatt tggaaaaaaa
     7201 tgatccatat tgacatcatt attccatttg tcgcataaat ccacttttta attttttcca
     7261 cattaatata atctcttatt taataaaatc aaaataaaaa tcaaatgcct aacaattcga
     7321 tctcaaattt tgaacgccaa cgtgtttgag cggtaacaat acatacttta agtttgcaat
     7381 tcttcatcgt cttatttttg ttttcatcaa ctatacgtct aaagcaacgc cgtccgtcat
     7441 tgttcggacg gcattttttc agactgcatt ttcccccgtt tgtgaaggcg cagggcgttg
     7501 cccaaaaccg ccagcgaact gaagctcata cccagtgcgg ctatccacgg ttggacatag
     7561 cccaaaacgg caagcggtac ggcaatgata ttgtacgcgc ccgcccatat caggttttgc
     7621 cggataatat ggcgggtgcg ccgcgcctga tcgagcaggt gggcgacggt acgcaaatct
     7681 tcgttcaata acacaatgtc cgcgccgtcc ctcgcaatat ccgtcccgcc cgctgcggcg
     7741 gcggatacgt ctgcctgcgc caaaacgggc gcgtcgttga tgccgtcgcc tatcatcagc
     7801 acttttttcc cttctttttg caaggctttg acgtattcca gtttgtcctc gggcatggct
     7861 tgggcgcggt agtgcgcgac acccagggcg cgtgcggttt cggcaacggc ggtttcgcgg
     7921 tcgccgctga ggatgtgcag ggtcaggttt ttgcctgcca actgccgcac cgcctccgcc
     7981 gcgctgtctt tcaaggggtc ggtcaggtag aacacggctt ggaaaccgct ttgactgccg
     8041 aggtaaaccg cgctgccgcc gccttctgtt tgcggttctt tacctgaaat ttcggcgaca
     8101 taggatgccc tgcccaatgc ccaaacctgt gtttcgccgt tgacggtcag ttgcgcgccc
     8161 acgccttcgc cgatgcggtt gaggcgttgt ttaatagcga tgtcggggac gctgccgtct
     8221 gaaatgcggc agttgaggat ggcgcgggca aggggatgtt cggactgttg ttctaaagcc
     8281 tgcgccaccg cgagaacaaa ggcttcgtct gtgcctctca acaatgagat acggcggacg
     8341 gcgggtttgc cttgggtcag cgtgccggtt ttgtcgaaga tgatgtcggt ggtttgggcg
     8401 agggtttcga ttgcctgctt tccgccgatt aaaatacctt cgcgcgccag cgtaccggta
     8461 gaagctgcca gcgcggtcgg cgtggcaagc gataaggcgc aggggcaggt aatgaccagc
     8521 agggcgacgg taatccacaa tgcggtgtgc gcgtcggcgt acagcgtcca gccgatgaag
     8581 acggggacgg caagcaggag ttcgccgaat atgaaagacg aggcgtattg ttctgccaac
     8641 tcggcagtgc gcggtttttg cgctaaggcg cggtcgagca ggcggacgat gtgcgacagt
     8701 cgcgtgccac cgccggtgcg gtcggtgcgt ataatcaggg ggctttgcgt gttgagtgtg
     8761 ccggcggtta ctttttcaga cggcattttg gcgacgggca ggctctcgcc ggtcagcata
     8821 gattcgttga cggcactgct tccttccagc accgtgccgt caacggggat ggtttcgccc
     8881 ggtttgacca gcacgatatc gcccgccttc aatttgacga cagctgcctc gcaggtttcc
     8941 tgcgtatcgg ggtaatcggg catatgatgg caaaacgcag gaatcagctt caccagcctc
     9001 tcggcggcat cgcctgcctt acggcgggca atgcgttcca taaagcgtcc gcccagcagg
     9061 aaaaacagca gcatcgcgat ggattcgaaa tacatcccct gccccgcatt tgtcgcaagg
     9121 ctgtaaacgc cggcgataaa ggtcatgatg atggcgacgg taatcggcgt atccatgccg
     9181 acgcggcggt ttttcaagtc gcgcagcgcg ccttgataaa acgggacggc gcaatagaat
     9241 acgacgggca gcaccattaa aaagccgccc caatggagga tttgcaggaa atcgggttcg
     9301 atgtcgccgc cgtaaaggta ggtcggcagc gcgaacatca tcgtctgcat catccccagc
     9361 ccggcaacgg cgaggcggac gatgtattgt ttgcgttctt tttggttggc ggcttcgatt
     9421 ttttgcgcgt catagggtgc ggcggtgtag cctatctgcc tgattttcaa cagaatgtcg
     9481 gaaaggcgga ttttgccgtc gtcccagacg acgcggcagc ggtgcgtgct gtaattgagg
     9541 tcgatgcgga cgatgccgtc tgtacgcaaa agctgctgtt cgatcagcca gacgcaggcg
     9601 gcgcaggtga tgccgccgag cattaaaacc gcctcgcgcg tgccgccgtg ggtttccaca
     9661 aagtcggact ggacttcggg caggtcgtac aggcggattt ggtcgaggat ttcttggggc
     9721 ggcagctcgg ttttttgcgc gtcggcggtg cgttgtttgt aataactgcc caagcccgcg
     9781 tcaataatgc tttgtgcgac cgcctgacag ccggcgcagc aggtttcgcg gtcttcgttt
     9841 tcgtaacgga cagtcaggtg gaggtgttcg ggaacatcca gaccgcagtg gaaacaggtt
     9901 tttttcatgg catttcggtt tcgtctgtgt ttggtgcggc ggcacaagac tcggcaatgg
     9961 cttcgcgcag tgcgtctata ccggtatttt cagcaacgga aatgcggacg gcggcaattt
    10021 ttcccgcagc gtcgcgccat atgcccgtgt tttgttcttc agacggcagc aggtcggttt
    10081 tgttgtacac cttgatgcac ggaatatcgc cggcatggat ttcccgcagt acgttttcca
    10141 cgtcttcaat ctgctgtccg ctgttcgggg cggcggcatc gacgacgtgc agcagcacat
    10201 cggcttgcgc ggtttcttcc agcgtggcgg aaaatgccga aatcagtttg tgcggcaaat
    10261 cactgacgaa tccgacggta tcggtcagga taatgctgca ttcgggactg atgtacagcc
    10321 gccgcgccgt ggtgtcgagt gtggcgaaaa gctggtcttt cgcatatatg cccgacttgg
    10381 tcagccggtt gaacaggctg gatttgccga cattggtata gccgaccagc gcaaacgttt
    10441 tgattgtgcc cgattcgcgg ggcttgcggc gcagggcgcg ctgttttttg aggttgacaa
    10501 gctgtttttt caaggcattg atccgatggg cgatcaatcg gcggtcggtt tccagtttgg
    10561 tttcgccggg gcctttcatg ccgataccgc cgcgctggct ctgcagatgg ccgtaaccgc
    10621 gtatcaagcg tcccgccaaa tggctcaatt gcgccaactc gacttgcagc ctgccttcct
    10681 gcgtgcgggc gcggcgggcg aaaatcgcca gaatcagccc taccctgtcc aatacgcggc
    10741 atttcagttc tttttcaagg ttgcgttcct gcgtgggcgt gagttcgtgg ttgaatacga
    10801 ccaaatcgat gccgtctgcg gcaactgctt ctgacagctc cgccgccttg cccgtgccga
    10861 caaacagcgc ggtgtgcgga cggtcgcgtt tggcagtctc cacgcgcacg gaatcgccgc
    10921 ccgccgcttt gaccagctcg acagcttccg ccaatgccgt ctgaaaaccg ttcagacggg
    10981 tggcactgct gcccgtgcca tctttgtcca acattacgcc caccagcatg acgcgttcgg
    11041 gttgcgcttg ggtactgccg ttgcggcctg ttctgcctgt catgtattca atcctgtaaa
    11101 taaagggtta cgcctcgttc cgtttctcct tccggtgcaa gcgtgatgcg gctgcccgcc
    11161 attccgtcaa cgctgaagac gatgccgccc gcctcgtgcc ttcctatgga caggcggctg
    11221 ccgggtcgct gccggtaggt ataacgggta cacgcttcca aacaccagcg tccgcgccag
    11281 tggatgaaat atttttcgcc ttttctaacc gcaagtgtct cgccgccgtt tacctcaaaa
    11341 taagggtaat cgggttttcg ggttctaaac tgccatttcg cctgcgcgcg ccgtccgttg
    11401 cggacatagt cgaataccgc cgtataaagc gtaccgtatt ccaaaggctt gagcgggaaa
    11461 agcgcgaatt ggtacgcggt cagcctgccg ttggggtcgt tgccggcggt taaaaccctg
    11521 acggggcgga tttcgttttt accctgatac agcttgaaac ttttcatcgt aattttgcct
    11581 gccgcctcgg aaaaatcaat gctggcagga ttgcccgtga tttcatattc cggcacgggg
    11641 tctggacgct cgccgtggaa ataaggcagt gcgccgttgc cgacgggata ggctgtatag
    11701 agcagctcct gtgcgggcat ggcttcgtcg gtgtacacga ccgcaccgtt atggcaggcg
    11761 ttgcggtaat atttccgtcc tgcttccggc tgatttctgc cttgggcgca atgccgctca
    11821 aacctgccgt tgccctgatt gaatacgaga acggttttac cgttttcgcg cacaaatgcc
    11881 gctcctgcct catccgtatg gcggtcaagt agggaaaggc ggtggtagat tgcgctcatt
    11941 aatccgtcca cttggcgttg ctgcgtgcgg atgtcgctgt cggacgattc ggcggcttcc
    12001 tcttccgtgc tgatgttttc atgcacgccg ttgtagagat acccggcaag gcgtgtgcgt
    12061 tcggtcagct tttgtgcggt gtagtgcgga ttgtcgggat ggtgttcgcc gtgtccgtct
    12121 tcgggattga gcgtgagata gcgtgcgtgc ctgcgggcgg aattttccaa aaccggcgcg
    12181 tgtgccagct tgtgcaaacc gatttgggct cggatgcggt tgaggtagtg cagcgcatcg
    12241 ccgccgtccc tgatttgcgg tgcggacgga tagacaagtt cgcccgcggg cagggattgg
    12301 ttttgggtat ggtagaaaac gcctgccgcc gaacccaaca ggagcagccg aataaaaagg
    12361 gatttcataa tgccgtcctc ccgacgcggc aacggggtgt tttatcaaaa cggcaaactt
    12421 gccaatgcgg tttccgattg cagattgcca aaccggcaaa cctttccccg tgccgatgcc
    12481 gaacccgatg ccgtctgaac cttcagacgg catcgggtgt ttatttgtcc acccggatgg
    12541 gggcaggttc gtattgtgtc gattcgtcgc cgtaatacag cacgccgagt ttgatgggaa
    12601 tgcgtccctg cgatttgcgg tgggcattgg aatcgcgcaa ggaataggcg caaccgcaat
    12661 attcctgctg gtagaagttt tcacgtttgc tgatttcaat catgcgcgcg ctgccgccgc
    12721 ctttgcgcca gttgaaatcc caatacacca catcatcgta aggcgcggcg gcacggcggc
    12781 cgcagtcgtt gatttgcgcc atatttttcc agcgtgaaat gcccagcgaa ctggtaaaga
    12841 cggggaaccc gtgttcatgc gcgtattgcg ccgccttttc aaaacgcata tcgaaacaca
    12901 tggtgcagcg gatgccgcgt tccggctcaa actccatgcc tttggctttg gcaaaccatt
    12961 ctttgcggtc gttttcgtag tcgtcgtctt tatcgatgaa aggaatgccg aacttttccg
    13021 caaagcgcac gttttcctct tttcggagca tatactcttt gtgcggatgg atattgggat
    13081 tgtaaaaata aatggtgtag tcgatgccgg aggcaagcat cgcctccatc acttcgccgc
    13141 tgcacggggc gcagcaggaa tgcagcaaga cttttttatg tccgccgggc gggacgagta
    13201 tggggcggtc aatgtcggta acggtaggtt tgttttgtgt ttccattaac ttaacaatat
    13261 ctgtcgaata cggttgaagc ggctatttta tcagagccgc cgcatgtaaa acagggggtc
    13321 agggcatcag cccgagctgc ctgaacgaac ggacggtttg cgccgttacg ataaaatggt
    13381 cgagcagcga cacatcgacc agcgacattg cctgtgccag ccgccttgtg aacatgatgt
    13441 cttcctgcga aggttcgggc gagccgcccg gatggttgtg cgcaataatc aggctgtcgg
    13501 catattcgtc caatgccagt ttgacgattt cgcggatgta aatcgtgttt tccgcaaccg
    13561 taccgcgcga cagctctctg accgcaatca gttggttttg gcggttcagc agcagcgcga
    13621 cgctgacttc gactttttcc tgccccaaat gaaagcgtaa ataatcggcc accgtatccg
    13681 gatcggacag gacgatgctc tcctgcaatt cttccgccaa tatccgccgc ccgatttccc
    13741 tgaccacggc aaactgtgtg aaacttgccg tccccatccc tttgtatgcc gacagttttc
    13801 cgacctccgc gctcatcagc ctccccaaac tgccgaactc ctgcagcaaa taacgcgcca
    13861 aatcgaccgc actcattccg cgcgtgccga cgcgcagcag gattgccaaa agttcggcat
    13921 cactcaacgc cgccgccccg cgttccaaca gcttttccct gggtctttcg ccttccggcc
    13981 attgcttgat gctcatgttt tccctttttt aaatagtctg agatgccgtc tgaaacagat
    14041 gttcccacac gccaaatatc cgaaatgccg tctgaaccgg atttccgctt cagacggcat
    14101 ctgtatttca aaacagtctt acgccgtttc ttccaactcg attgaagccg ccagaagcga
    14161 caggcgtgcc attacgccgt aaacgtagag gcggttgtgt tcgttgtcca cgtcgcagtc
    14221 tttttcagag cgcggcatac ccagcgagtc ccattgttcg aatacgcgct tgcaggcttc
    14281 gggcgcttct tgggaattat cgccgttacc ggtaggaatg ctgttagaca gcggaacaaa
    14341 caccataccg ccggcgttga ggttttcgtc cgcaccgcgc ccttcgtgta cgcggaaaaa
    14401 gccgccgatg acgaagcggt ccatcatata cacgacgggt tcgcacaccg cgccgtttaa
    14461 ggtttcataa gtataaatcc cttcttggac aatcacttcg ctgacttcca agccttcttt
    14521 gactttcgcc attttattgc ggtttttacg gttcaatccg cgcacttcgt cggcggattt
    14581 gacgctcatc acgcccatgc cgtaagtgcc ggcatcggct ttgacgatga cgaaaggttt
    14641 gtcggtaatg cccgattcgt cgtatttggc ttgaattttc gccagcacac gttctaccgc
    14701 ttccgccaac gcgtcttcgc cttcacgccc ttggaagtcc aaaccgccga ttttttcaaa
    14761 ataagggtta atttgccatt cgtcgatgcc gattaacttg gcaaattcgg cggtaacttg
    14821 gttgtacgcg ccgaaatgat ttgttttgcg gcgcgtcgtc caaccgccgt gcaacggcgg
    14881 caaaacggtt tggctgatgc ctttgaggat gtcgggaatg ccggcagaca aatcgttgtt
    14941 caacaaaacc acgcaaggcg aaaagccgtc tgcaagatgg acgcggtcgc gggtacgcaa
    15001 taaaggttcc aacaggattt tgtcgcccaa tgcagtttca aattcggtcg gttcggttac
    15061 ttccggattc aggctgccca agcgcacttc ataccctgcc gaacgcaaaa tctcgccgag
    15121 ggcgtaaatg ttttgcaggt aaaacgtatt gcgcgtgtgg ttttcgggaa taatcaatac
    15181 ggatttcgcc gtttcgcagg cgcgttgcac cgcatcttgc gcggcaaccg ccgccagcgg
    15241 gataaagttg ggattcagat tattgaagcc gccggggaac aaattcatat cgatagacga
    15301 aattttgtaa ccggcattgc gtatatcgac cgaaccgtaa aacggcgggc ggtgtacgct
    15361 ccactgcgtg cggaaccacg cctcgatttt ggcgtgattg gacaggattt ttgcctcaaa
    15421 cgcctgaagt tgcgccgaat gttcgggcga catcaccggt aatttcatct ttcgtctgcc
    15481 tctgtgtgtt ttttgacaag atgcagatga taatgccttt gaaagaatga cgacaagtat
    15541 tctttcgtat cgaatcatgc tttagatata actgtttact gaaatggaca atacgtctaa
    15601 ttttttcgtt aaaattgttt gcaaaataac accggctcaa aaattagaca aaatttgttg
    15661 cgcggtataa agaatacgtc taaaatccgc ccaatccgca ttaccattta tccaaaagaa
    15721 caacatcatg acagcacaaa ccctctacga caaactttgg aacagccacg tcgtccgcga
    15781 agaagaagac ggcaccgtcc tgctctacat cgaccgccat ttggtgcacg aagttaccag
    15841 ccctcaggca tttgaaggct tgaaaatggc ggggcgcaag ctgtggcgca tcgacagcgt
    15901 cgtctccacc gccgaccaca acaccccgac cggcgattgg gacaaaggca tccaagaccc
    15961 gatttccaag ctgcaagtcg atactttgga caaaaacatt aaagagtttg gcgcactcgc
    16021 ctattttccg tttatggaca aaggtcaggg catcgtacac gttatgggcc ccgaacaagg
    16081 cgcgaccctg cccggtatga ccgtcgtctg cggcgactcg cacacttcca cccacggcgc
    16141 attcggcgca ctggcgcacg gcatcggcac ttccgaagtc gagcacacga tggcgaccca
    16201 gtgcattacc gcgaaaaaat ccaaatccat gctgattgcc gttgacggca aattaaaagc
    16261 gggcgttacc gccaaagacg tggcacttta catcatcggg caaatcggca cggcgggcgg
    16321 tacaggctac gccgtcgagt ttggcggcga agccatccgc agcctttcta tggaaggccg
    16381 catgacttta tgcaatatgg cgatcgaagc cggtgcgcgc tcaggcatgg ttgccgtcga
    16441 ccaaaccacc atcgactatg taaaagataa acccttcgca cccgaaggcg aagcgtggga
    16501 caaagccgtc gagtactggc gcacgctggt gtctgacgaa ggtgcggtat tcgacaaaga
    16561 ataccgtttc aacgccgaag acatcgaacc gcaagtcact tggggtacct cgcctgaaat
    16621 ggttttagac atcagcagca aagtgccgaa tcctgccgaa gaaaccgatc cggtcaaacg
    16681 cagcggtatg gaacgcgccc ttgaatacat gggcttggaa gccggtacgc cattaaacga
    16741 aatccccgtc gacatcgtat tcatcggctc ttgcaccaac agccgcatcg aagacttgcg
    16801 cgaagccgcc gccatcgcca aagaccgcaa aaaagccgcc aacgtacagc gcgtgttaat
    16861 cgtccccggc tccggtttgg ttaaagaaca agccgaaaaa gaaggcttgg acaaaatttt
    16921 cattgaagcc ggttttgaat ggcgcgaacc gggctgttca atgtgtctcg ccatgaacgc
    16981 cgaccgcctc gcaccggggc aacgctgcgc ctccacctcc aaccgcaatt ttgaaggccg
    17041 tcaaggcaac ggcggacgta cccacctcgt cagccctgcc atggcagccg cagccgcagt
    17101 gaccggtcgc tttaccgaca tccgcatgat ggcgtaagta ttttcaggcg gtctttaaga
    17161 atttgacgcg gccgtctgaa atatgtcccg taaggcagaa acacctttaa tattcaaacc
    17221 aaggagttta accatgaaca aacttttcat taccgccctg tccgccctcg ccttgtccgc
    17281 ctgcgccggc acttgggagg gcgcgaaaca agacaccgcc cgcaaccttg acaaaacaca
    17341 ggccgccgcc gaacgcgccg ccgaacaaac aggcaacgcc gtcgaaaaag gctgggacaa
    17401 aaccaaagaa gccgtcaaaa aaggcggcaa tgccgtcgga cgcggcattt cccatctcgg
    17461 cggaaaaatc gaaaacgcca ccgaataacc gattcgcgat gccgtctgaa accgcttcag
    17521 acggcatcgt cagcacaaga cgcaaaccca tgaaagcctt taccaaaatt accgccatcg
    17581 tcgccccgct cgaccgcagc aacgtcgata ccgacgccat catccccaaa caatttttaa
    17641 aatccatcaa acgcagcggc ttcggcccca atgcctttga cgaatggcgt tacctcgacc
    17701 acggcgaacc gggcatggac aacagcaaac gcccgttgaa ccctgatttc tcgctgaacc
    17761 agccgcgtta ccaaggcgcg caaatcctgt tgacgcgtaa aaacttcggt tgcggctctt
    17821 cacgcgaaca cgccccttgg gcattggacg actacggctt ccgcgccgtc atcgccccca
    17881 gcttcgccga catcttcttt aacaactgct acaaaaacgg ccttttacct atcgtgttga
    17941 ccgaagaaca agtcgatcgg cttttcaaag aagttgaagc caacgaaggc tatcagctct
    18001 ccatcgacct tgccgaacaa accctgacca caccaagcgg cgaaacattc acattcgaca
    18061 ttaccgaaca ccgcaaacac tgcctcttaa acggcttgga tgaaatcggt ctgaccctgc
    18121 aacacgccga cgaaattcac gccttcgaag aaaaacgccg ccaaagccag ccttggctgt
    18181 ttaacggtta agcctatgcc gtctgaagac cgttcagacg gcattcctga taacatataa
    18241 ttgctgcaac taatgtaaac agaggggcta taaattgctt tcatctttca agcgggctta
    18301 gcaacgctct aaattaatgc aatacagctc tatttcaaat aaatttttag atttttaata
    18361 tgcaacagat taaatttatt gacttatttt ccgggatgag cggtatcagg aaaggatttg
    18421 aacaagcctg tcggaaacag tcggttgctt gcgaatgtgt ttttacctca gaaatcaaac
    18481 cggcagcttt ggaagtattg aagcaaaact accctgatga agtgccgtat ggagatataa
    18541 cgaaaattga aacgggagat attcccgatt ttgacatctt gttggcaggc ttcccttgcc
    18601 aggctttttc tttcgccgga aaaagattgg gctttgaaga tacgcgggga acgcttttct
    18661 ttgatgtggc aaggatttta aaggcaaaaa aaccaaaagg ttttattttg gaaaatgtgg
    18721 aaggattggt gacgcacgat agaaaagatc cgacacaaaa aataggacgc accctgaccg
    18781 ttattttgga aaccttggaa acattgggct actatgtttc ttggaaagtt ttaaatgcaa
    18841 aagatttcgg cattccccaa aacaggaagc gcatttatct gacaggcagt ttgaaatcca
    18901 aacccgactt gtcttttgaa acaaccctaa gtccgaaatt aaaaaatatt ttggaatcgg
    18961 gactgcctac cgaaagcagt cctttcatca aaaaattgct aaagaaattt cccccgtccg
    19021 aactgtacgg aaaatcagta aaggacaaac ggggagggaa aaacaatatc cacagttggg
    19081 atattgaatt aaaaggcaca gtaaccgaag aagagaagca attattaaat atgcttctaa
    19141 aagaacggag gaagaaaaaa tgggcttcag aaatcggcat cgattggatg gatgggatgc
    19201 ctttgacaaa agcgcaaatt tcaactttct ataaacaccc caatcttcaa aatattttgg
    19261 acagcctgac agataaaggc tatttggttt tggagcatcc gaaacaaaaa attggcgggc
    19321 gaagaatcaa agatgaatcc ctgcccaaag gctacaatat tgtttcagga aaaaaatcat
    19381 ttgaaatcaa taaaatatta gacccaaatg atgttgcgcc aaccttagtt gcaatggata
    19441 tggagcacct tttcgtcgtt gacaacggcg gtttgcgcac acttaccgga aaagaaggtt
    19501 tacgcttatt cggctatccg gacgattatt cgtttgatat tcctaaaaaa gacaaatacg
    19561 atttattggg caataccgtt gccgttcctg tgattaaggc ggtatctgaa aggcttctgc
    19621 atactttata aaccgatagg gataaaatgg taaaacttac tgcacaacaa atatttgata
    19681 agttgctgaa tgaggaaaaa atcttatcgg tcaatggtca aattagattt ttcttgggcg
    19741 atgtggatat tatcgtcaaa caaaaagatg tcgtcggcaa catcattcaa gaatggctcg
    19801 gcggatggtt gagaaaaagg gaaattgaat ttgatgtttc aaccaatacc caaatgcccc
    19861 ccgatttctt tttaaataaa aaagaccgca gtagagaatt gttggaagta aaggcgttca
    19921 accgaaatgc cggcccgggt tttgatattg cagattttaa aatgtattct gatgaaatca
    19981 ttcataaacc ctatatgctg gatgtagatt atttaatatt cggttacgat atggacgaca
    20041 acggcaatgt aaccatcaag gatttatggc ttaaaaaagt atggcaaatt acgagaagca
    20101 tggatgggtg ggcaattaat cttcaagtca aaaaaggcgt ggtgcataaa atccgcccgg
    20161 gtgtttggta cagcataaat aaaaagaata tgcctatgtt tgaatgcttg gaagattttg
    20221 tttccgcaat tgaagaaacc gtttatcaaa acccggcaac acggcataac gcatccttat
    20281 ggaaaaagaa atttgaggaa gcctataaaa aacactacaa ccgatcgatt tccatacctc
    20341 gttggcacga aattgcacac aaatacaaaa agaaataacc cttcaaccat caacccataa
    20401 ggaaacttga aatgaccaaa catatcgcca tcctccgagg tgacggcatc ggtcccgaaa
    20461 tcgtcgccga aaccgtccgc gtactcgaca aactcatcgc ccaaggcttg gacatcagct
    20521 acgaatacgc cccattgggt ggtgaagcct acgacgaata cggccatcct tatcccgaat
    20581 ttacgcaaaa cctctgccgc aaagccgatg ccgtcctgct tggtgcagtc ggttctcctc
    20641 aatacgacaa cctcgaccgt ccgttgcgcc ctgagcgcgg cctgttggca atccgtaagg
    20701 atttgaacct gtttgccaat ttgcgtcctg ctgttttata tcccgaatta gccaacgcct
    20761 ccacgctgaa gcctgaaatc gttgccggtt tggatatttt gattgtgcgc gaactcaccg
    20821 gcgacatcta tttcggcgaa ccgcgcggca tccgcgttct ggaaaacggc gaacgcgaag
    20881 gctacaacac catgaaatac agcgaaagcg aaatccgccg tattgcccat gttgccttcc
    20941 aatccgccca aaaacgcagc aaaaaagtct gttccgtagg caaagccaac gttttggaaa
    21001 ccaccgaact gtggcgcgaa atctttgaag aaatcggcaa acaatatccc gatgtcgaac
    21061 tctcccatat gtatgtggac aacgccgcta tgcagctcgt gcgcgcgccc aaacaatttg
    21121 acgtgattgc caccggcaac atcttcggcg atattttatc ggacgaggcc tctatgctga
    21181 ccggctccat cggtatgctg ccttctgctt ctttggacga aaacggcaaa ggcctgtacg
    21241 aaccgtctca cggctccgct cccgacatcg ccggacaaaa caaagccaac ccgctggcca
    21301 ccatcctctc gctcgcaatg ttgctgcgtt acagcctgaa cgacgaagcg cgcgcgcaac
    21361 aagttgaaaa cgccgtccaa aaagtgctgc aacaaggctt gcgtaccagc gacatttacg
    21421 aagaaggcac aaaactcgtt tcctgctccg aaatgggcga tgcgatactc gccgccttgt
    21481 aaaaaaacaa agcgcggatc acaccgcgct ttttcatctg cccttttccg cccgcaccgt
    21541 tcgtcaaccg cctgcaaacc caccgtttac cggcaggaaa tgccataaat ccccgcctat
    21601 gttaggataa acacctttat cggcccacac aaaggattat catcatgaaa aaaatcatct
    21661 tcgccgcact cgcagcggca gccgttggca ctgcctccgc cgccacctac aaagtggacg
    21721 aatatcacgc caacgcccgt ttcgccatcg accatttcaa caccagcacc aacgtcggcg
    21781 gtttttacgg tctgaccggt tccgtcgagt tcgaccaagc aaaacgcgac ggtaaaatcg
    21841 acatcaccat ccccgttgcc aacctgcaaa gcggttcgca acactttacc gaccacctga
    21901 aatcagccga catcttcgat gccgcccaat atccggacat ccgctttgtt tccaccaaat
    21961 tcaacttcaa cggcaaaaaa ctggtttccg ttgacggcaa cctgaccatg cacggcaaaa
    22021 ccgcccccgt caaactcaaa gccgaaaaat tcaactgcta ccaaagcccg atggcgaaaa
    22081 ccgaagtttg cggcggcgac ttcagcgcca ccatcgaccg caccaaatgg ggcgtggact
    22141 acctcgttaa cgttggtatg accaaaagcg tccgcatcga catccaaatc gaggcagcca
    22201 aacaataaat cttgatgcga atcagcacaa atgccgtctt catcaaaccg ccccgccaac
    22261 ggtaagccga tatggcggag cacaatccgc cgaaagatat ttcagcggca ggcagggctt
    22321 gccggaaagt ccggaaa
//
LOCUS       DAWFAN010000013        21718 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWFAN010000013 DAWFAN010000000
VERSION     DAWFAN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.013"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(84..1970)
                     /gene="uvrC"
                     /locus_tag="WGO65_000348"
     CDS             complement(84..1970)
                     /gene="uvrC"
                     /locus_tag="WGO65_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ0326700.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPMPDALKEALESEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     EHLH"
     gene            complement(2025..4202)
                     /locus_tag="WGO65_000349"
     CDS             complement(2025..4202)
                     /locus_tag="WGO65_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ0326701.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVKSAGVNFASEEAQ
                     VTFDDSKTSAADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRDGEWRQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGIGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKA
                     GKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            4341..5291
                     /gene="trxB"
                     /locus_tag="WGO65_000350"
     CDS             4341..5291
                     /gene="trxB"
                     /locus_tag="WGO65_000350"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ0326702.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5447..5815
                     /gene="rpsF"
                     /locus_tag="WGO65_000351"
     CDS             5447..5815
                     /gene="rpsF"
                     /locus_tag="WGO65_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ0326703.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5816..6118
                     /gene="priB"
                     /locus_tag="WGO65_000352"
     CDS             5816..6118
                     /gene="priB"
                     /locus_tag="WGO65_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ0326704.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHKSWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            6125..6355
                     /locus_tag="WGO65_000353"
     CDS             6125..6355
                     /locus_tag="WGO65_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ0326705.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6372..6824
                     /locus_tag="WGO65_000354"
     CDS             6372..6824
                     /locus_tag="WGO65_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ0326706.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            6838..6945
                     /locus_tag="WGO65_000355"
     CDS             6838..6945
                     /locus_tag="WGO65_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ0326707.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            7525..7680
                     /locus_tag="WGO65_000356"
     CDS             7525..7680
                     /locus_tag="WGO65_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326708.1"
                     /translation="MYTGHYGGNTVWEAAASRGIGTAGDAPMPSGVKAGQSAVWKKAM
                     ESVGRRS"
     gene            complement(7796..8602)
                     /locus_tag="WGO65_000357"
     CDS             complement(7796..8602)
                     /locus_tag="WGO65_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0326709.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(8603..9349)
                     /gene="pssA"
                     /locus_tag="WGO65_000358"
     CDS             complement(8603..9349)
                     /gene="pssA"
                     /locus_tag="WGO65_000358"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ0326710.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(9404..9826)
                     /locus_tag="WGO65_000359"
                     /pseudo
     CDS             complement(9404..9826)
                     /locus_tag="WGO65_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9932..10414
                     /locus_tag="WGO65_000360"
     CDS             9932..10414
                     /locus_tag="WGO65_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ0326711.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(10466..11677)
                     /locus_tag="WGO65_000361"
     CDS             complement(10466..11677)
                     /locus_tag="WGO65_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ0326712.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            11900..14338
                     /locus_tag="WGO65_000362"
     CDS             11900..14338
                     /locus_tag="WGO65_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ0326713.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNSKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDSPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            14547..15857
                     /locus_tag="WGO65_000363"
     CDS             14547..15857
                     /locus_tag="WGO65_000363"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ0326714.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKVPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQVPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15953..16567
                     /gene="clpP"
                     /locus_tag="WGO65_000364"
     CDS             15953..16567
                     /gene="clpP"
                     /locus_tag="WGO65_000364"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ0326715.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQL"
     gene            complement(16621..17886)
                     /gene="ispG"
                     /locus_tag="WGO65_000365"
                     /gene_synonym="gcpE"
     CDS             complement(16621..17886)
                     /gene="ispG"
                     /locus_tag="WGO65_000365"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ0326716.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKITLKGDNIASEFL
                     AIVEEYVKTNYGENSSKRNKGKVIPIQSL"
     gene            complement(17902..18663)
                     /gene="pilW"
                     /locus_tag="WGO65_000366"
     CDS             complement(17902..18663)
                     /gene="pilW"
                     /locus_tag="WGO65_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ0326717.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(18666..19760)
                     /gene="rlmN"
                     /locus_tag="WGO65_000367"
     CDS             complement(18666..19760)
                     /gene="rlmN"
                     /locus_tag="WGO65_000367"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ0326718.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMIPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19904..20329)
                     /gene="ndk"
                     /locus_tag="WGO65_000368"
     CDS             complement(19904..20329)
                     /gene="ndk"
                     /locus_tag="WGO65_000368"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ0326719.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPAEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(20388..21539)
                     /locus_tag="WGO65_000369"
     CDS             complement(20388..21539)
                     /locus_tag="WGO65_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ0326720.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKDSGG"
BASE COUNT         5200 a         5367 c         5904 g         5247 t
ORIGIN      
        1 ggtttaaatt taatccacta tatatcggta taccgacctg ctgtatcata ttttttacag
       61 acggtttttg cttgggcagc atactaatgc agatgctcgt aaatggtttc cgccaatgcc
      121 ttgctgatgc cttccacttt ttccaagtcc tcgcggctgg cggcaatcac gccgcgcaga
      181 ccgccgaagc gggtgagcag ggcttggcgg cgtttgctgc ctacgccggg gatgtcgctt
      241 aaggaggagg taacgcgggc tttgtcgcgt tttttgcggt ggccggtaat ggcgaaacgg
      301 tgcgattcat cgcgtacggt ttgcaataga tgcaaggccg ggctgttggg cggcaggcgg
      361 aagacttcgc cggtaaaagg cagtatgagt tcttccatac cggctttgcg ctccgggcct
      421 ttggcgatac cgaccaaagg aatgtgcagc ccgagttctt cccataccga tacggcgatg
      481 ccgatttgcc ctttgccgcc gtcaattaac acggcatccg gccatttgac ggtctcgccg
      541 ttggcttcgg cttcctgcat tttgccgtaa cggcgcgtca acacttcgcg catggcggcg
      601 tagtcgtcgc cgggtttggc ggtcgtgatg ttgtagcggc ggtattgcga gggctggatg
      661 ttttgctcat cgtacacgac gcaggaggcg atggtggctt cgccttgggt gtggctgatg
      721 tcgaagcatt cgaggcggtt gaggccgtct gaatccatac cgaggatttt ggcgagttcg
      781 tcgatgcggt gttgctggct gctttgttgc aggcggcgtt gtgcaatcgc catttgtgcg
      841 ttttgctccg ccattttcaa ccaaactttg cgttcgccta tggtcttggt gacaaattgc
      901 atttgtttgc cgtgttcgct ctctagggct tctttcaatg cgtcgggcat ggggaagttg
      961 ctgatgatga tgtcaggttt gcttttgccc agatagtgtt gggcgacgaa ggcttcggca
     1021 tagtcttgcc catttggctc gggatcgttt ttggtgtcgg ggaagaaact tttgtctccg
     1081 acgtgccgcc caccgcgtat gctgacccag tgtacgcaga cgatgccgtc tgaaaccgcc
     1141 agcgcgagca aatcgatatc gttggggttg ttcgggtttt tgctgtcgat gaactggttg
     1201 ctctgcataa tgccgagtgc ttggatttgg tcgcggtagc gggcggcttc ttcaaattgc
     1261 agattagcgg cggcagtttg cattttgtgt tgcagggtgc gggtcagttc gtcggttttg
     1321 ccattgagaa aagtggcggc ttgacgcacg ctgtcgcggt agtcttcttc gctgatgtgt
     1381 ccgacgcagg gcgcggtgca gcgtttgatt tgatacagca ggcaagggcg gtcacgatgc
     1441 tcgaatacgc tgtcttcgca ggtacgcagc ataaagactt tttgcaacac ttgaatgctg
     1501 tcgcgcacgg cgttgctgtt gggatacggg ccgaagtatt ggtttggctt tttcagcgtg
     1561 ccgcggtaat acgccatctg cggatattga tggccgctga gcatcaaata aggatagctt
     1621 ttgtcgtcgc ggaaaaggat attgtatttc ggcgacaagg ctttgatgag gttgttttcg
     1681 agaatcaggg cttcggcttc ggaacgcgtg atggtggttt cgatatgatg aacctgtttt
     1741 accatcaatg cgatgcgcgg cgaatggtcg tttttttgga aatagccgga cacgcgccgc
     1801 ttgaggttga ccgctttgcc gacgtataag actttgccgc cttcgtcaaa aaaacggtac
     1861 acgcccggca gcttgggcag gtttttgagg aagagcggga tatcgaattg ttccggagaa
     1921 cggttttctg tattcatatc aattatttga aaaacggctt cagacggcat atcggcagcg
     1981 gcagccttat ccgttcggaa aagctgtcgg gtggttatat gcggtcaatc gatttttacc
     2041 cgtttcaggc gcaaggcgtt gctcaacacg gaaaccgagc ttgccgccat tgccgcgcct
     2101 gcgatgacag ggtttaaaaa accgagcgcg gcaagcggaa tgcccaaaat attgtagaag
     2161 aaggcgaaaa acaggttttg cttgatgttt ctcaacgtcg cccgcgatac cgatagggca
     2221 tcggcgagtt ggttgaccga atgttgcatc agcgtggcgg atgcggtgtg ttcggcaacg
     2281 tccgcgccgc ctttcatggc gaagctaacg ttggcggcgg cgagcgcagg tgcgtcgttg
     2341 atgccgtctc cgaccatcgc cacggttttg ccggcggctt tgagtttctg cacttcggcg
     2401 gctttgtcgc gcggactcat attaccgaag gcgtgtgcga tgcccagttg tttggcgacg
     2461 tattcgaccg tgccttggtt gtcgccgctc ataatataga catcgatatt gtgttttttc
     2521 agacggccta tggcttcggc ggtatcggct ttcaacgcgt cggcaagtgc gaatgcgccg
     2581 atgggtttgt tatcgactga gaccgcaaca atgcttgcaa tatcccaaac gccgtctaaa
     2641 aacttcggca aggccagttc ggcaaattcg gctttgcctg ctttcaccaa acccacgcct
     2701 tccacttcgg cggtaatgcc tgcgccgaca acggtttgtg cgttttgtgc ggcgggaatg
     2761 tccaaaccgc gcgcttgggc ggcggagacg atggcacggg cgagcggatg ggcggcgttt
     2821 tgttcgacgg cggcggcgat gcggtacaaa gcgtcttcgt caaagccgct gtcgggaacg
     2881 caataaacgg cggcaacctg cgggctgcct tcggtcagcg taccggtttt gtccaacacg
     2941 acggcatcga cgtgggcggc ttcctccatt gctgccgcgt ctttaaacca aataccgtgt
     3001 ttcaccgctt tgccaatgcc gaccataatc gcggcgggtg tagccagacc gagcgcacac
     3061 gggcaggcaa tcaccaaaac ggcgacggcg tgcatcagcg caaccgtcca atcgccctta
     3121 atcagccaag taacaataaa agtcaacaac gcgatgccca cgacggcagg cacgaacacc
     3181 gccgccgcct tgtccgccac gcgcgcaatc ggtgctttac tgccttgcgc ttcagagagt
     3241 gcgttcatca tgtcgccgag cagggtttgg ctgccgagct gcgcggcgcg gtacaccacg
     3301 ctgccttcag tcatcagcgc gcccgccaat actttgccgc ctgccttttt ctcttcggga
     3361 ttggattcgc cggtaagatg gctttcgtcc gcccagccgc tgccgctttc tatgatgccg
     3421 tcggcggcaa tgcgttcgcc gtgattggcg cggattaggt cgccgatttg cacttggtcg
     3481 atgggtagct gccgccattc gccatcgcgt tgcacgttga cttgggttgg cgtgagtttg
     3541 agcagcaagc ccaagctgtt caggctggat tttttggtgc ggtgttccaa aaatttaccc
     3601 agtgacacaa aaccaatcac cattatgcct acttcaaaat acacatgcgc cataccgtac
     3661 gccgcgtgcg ggctgaaaaa cagcatatag acggaataca ggtaaatcga gaccgtgccg
     3721 atggtaacga gtacgtccat attcgccagc ccgcctttaa tgctcgccca cgcgcttttg
     3781 taaaatggca ccgccagcca aagctgcacc acactcgcca atgcaaactg caacaagggc
     3841 gacaacatcc aatcatgccg tgtccaattc agccctttta gcatcatccc taccataccg
     3901 ataaggaacg ggatattgat ggccagcaaa agccacaacc tccagccgat atggtgttct
     3961 gcttcgggtt gcggcaatgt atcttccgtc ttttccttcg cgccgtaacc ggttttctca
     4021 atgattttgg caatgtcggc agcggaagtt ttgctgtcgt caaacgtaac ctgcgcctcc
     4081 tcgctggcga agttcactcc cgctgatttg acaaagtctt ttttgttcaa cactttttca
     4141 atgcgcgaag cacatgcctg acaggtcatg ccttcgattt ggaaacggat tttttgttgc
     4201 ataagcgtga ttcctgcttt gatattcaga cggcagttaa ggctgcggga tgccgtctga
     4261 gcgccggtat ggtcgaaatt tctcaaacgc gccgataatt cgagtagaat ccgaaaactt
     4321 gacacagcaa aggaaaaaca atgtcccaac accgcaaact gattattttg ggttccggcc
     4381 ccgccggata caccgccgcc gtctatgccg cgcgtgccaa tttaaacccc gtcattatta
     4441 caggtatcgc gcaaggcggg caactgatga ccaccaccga agtggacaac tggcctgccg
     4501 atgccgacgg cgtgcaaggg ccggaattga tggcgcggtt tctcgcccac gccgaacgtt
     4561 tcggaacgga aatcattttt gaccaaatca acgccgtcga tctgcaaaaa cgcccgttta
     4621 cactcaaagg cgatatgggc gagtacactt gcgatgccct gattgtcgca acaggcgcgt
     4681 ccgccaaata cctcggtttg ccgagtgagg aagcgtttgc aggaaaaggc gtttccgctt
     4741 gtgccacctg cgacggtttc ttctacaaaa accaagatgt tgccgtagtt ggcggcggca
     4801 atacggcagt tgaagaggca ctctaccttg ccaatatcgc caaaaccgtt accctgatcc
     4861 accgccgcag cgagttccgt gccgaaaaaa tcatgatcga caaactgatg aaacgcgtgg
     4921 aagagggcaa aatcatcctc aagctggaaa gcaacctgca agaagtactg ggcgacgatc
     4981 ggggcgtaaa cggcgcatta ttaaaaaaca acgacggttc tgagcaacaa attgccgtca
     5041 gcggcatttt tatcgccatc gggcacaagc cgaataccga tattttcaaa ggtcagttgg
     5101 aaatggacga agccggttac ctgaaaacca aaggcggcac ggcggacaat gtcggcgcaa
     5161 ccaatatcga aggcgtatgg gcggcgggcg acgtaaaaga ccatacctac cgtcaggcga
     5221 ttaccagcgc ggcttccggc tgtcaggcgg ctttggacgc ggaacgctgg ctgggcagcc
     5281 aaaacatttg aatgccgtct gaaaccgaaa ttttcggcgg aatgcaagtt tttcgcacca
     5341 aatccttgat tccgtcggca aaccccaata taatgcccgt tctttgaacc ttgccttctg
     5401 ctttcgcatg gcagaaggct gtttttacca tccataagga gataacatgc gtcattacga
     5461 gatcgtgttt atcgttcatc ctgatcaaag cgagcaagtg cccgctatgg ttgaacgtta
     5521 caaaaccatg attgctgaag caaacggtaa gattcaccgt ttggaagatt ggggtcgccg
     5581 ccaactggct tacccgatta acaaaatcca taaagcccat tacgttttga tgaacatcga
     5641 aaccactccc gaagtggttg aagagctgga aaccgcattc cgcttcaatg atgccgtatt
     5701 gcgccatcta accatcaaaa ccaaacacgc cgttaccgaa gcatccccta tgttgggtgg
     5761 tgaaaaggct aagaacctgt tgagcggtgc gtctgaagaa gcggtcgccc aataattggg
     5821 attcaataat cttgtttcgc ttgccgcgtt aattgaaaag gttttcccta ttcgatatac
     5881 gcctgccgga atccctgttt tagatattat tttaaagcac aaatcgtggc aggaggaaaa
     5941 cgggcagcaa tgccttgtcc aattggaaat tccggcacgg attttaggca ggcaggcgga
     6001 agagtggcag tatcggcaag gtgtatatgt tcacgtcgaa ggttttttag ctcaaaaaag
     6061 cagacgttcc cttatgccga tgctcaggat acaaaatatt caagaatata aaggttaaac
     6121 gacaatggct cgtcaatcat tcaaacgtag aaaattctgc cgtttcacgg ctgaaaaaat
     6181 ccaagaagtc gattacaaac aagttgattt gctgaaagac tttatctctg aaaacggtaa
     6241 aatcattcct gcacgcatca caggaacgaa ggcattctac caacgccaat tggctgttgc
     6301 cgtaaaacgc gcacgcttcc tggctctgct gccttacacc gaccaacaca aataattttg
     6361 gagattgaat catgcaaatt attctgttag aaaaaatcgg cggtctgggc aacttgggcg
     6421 acatcgtaac cgttaaaaac ggctacgccc gcaactttct aattcccgca ggtaaggcaa
     6481 aacgtgcgac cgaagcgaat atgaaagagt ttgaagcacg ccgtgccgaa ttggaagcca
     6541 aacaggctga aattttggca gatgcccgag tccgtcagga aaaattggac ggtcaaaccg
     6601 ttaccgttgc tcaaaaagcc ggtgtggacg gccgcctgtt cggttccgtt accaatgccg
     6661 acattgctgc tgcaatcgtt gctgccggca tcgaagccgt gaaagcaaat gtacgtctgc
     6721 cgaacggtcc tctgaaagcc gttggcgagt acgaagtgga agtggctttg cacactgatg
     6781 ccgttgctaa aattaccgtt gccgtcgttg ccgcaaccga gtaattgaaa ttatcagatg
     6841 ccgtctgaaa ccctgccgat tgtcggaatg tggtttcaga cggtatttgt tttgttttcc
     6901 gtatattctg caaaaatcgg gatggttgtt tttatagtgg attaaattta aaccagtaca
     6961 gcgttgcctc gccttgcctt gccttgccgt gctatctgta ttgtctgcgg cttcgtcgcc
     7021 ttgtcctgat ttttgttaat ccactatatt tgcctgtggt ctttgcagga atagtttgtt
     7081 aacgaaattt gatgcataaa atgcgccaaa aaatattggc tgtgttttaa tatagtgttg
     7141 ataagcgtac ctggcttcaa tatactcaat aggatagatc tcttcctaac cgaaaaaata
     7201 attgtctttt cccttgcaaa taattgaaat caacagatta ctaaaacaca gccaaaatat
     7261 tcaattgaca aaaacttttc tgattttgag aaatctgtga aaatcagggg attatttatt
     7321 ttgaaaatgg tatcgggcat aaaatttggc tgtgtttcaa tatggtgctg ataacatact
     7381 ttgtttcaaa atgttcaata ggataacttc ctgattgacc gaaaaataat ggccttttcc
     7441 cttgcaagca gttgaaatca acagattgtc aaaatacatc ctaaaaattt aataacttat
     7501 atatatgatt gcaaaggttg cggcttgtat accggtcatt atggcggaaa taccgtttgg
     7561 gaggcagccg catcgcgtgg aatcggcacg gcaggcgatg cgccgatgcc ttcgggcgtg
     7621 aaggcggggc aatcggcagt ttggaaaaag gcaatggaat ctgtgggcag aagaagttaa
     7681 gggtaaaagt cgggcgcacg gtcaggttgt ctgtcctttt gtcggaacag gagactgggc
     7741 agctttgtcc tgattaattg cttgtgcaaa cggtaaaaag gttttttcgt gtgttttaaa
     7801 gcaggttgta cagcattttt ccggcaatca aaagcaacat aatgccgaag gattttttga
     7861 gtttggcaga agaaagtttg tgggcggttt tgacaccgag cggggcaaag gcaatggttg
     7921 ccgcgctgag gacggcgacg gcgggcaggt aaaggaagcc cagtgaccct tcgggcaatc
     7981 ctgcaatatt caggccgttg agcagatacg atattgcgcc ggagagtgca atcggccagg
     8041 caaggccgga tgatgtgccg atggctttat gggcggggaa gccgcagtgg attaagaagg
     8101 ggacggaaag tgaaccgccg cctatgccga cccagctcga cattgcgccg aacagtgtgg
     8161 aaaccgcagt cagtccgggc agtccgggca gcgggcggga tgccgtctga gggtcggtat
     8221 gcagtgtttt gaatgcgacg gcggttaaaa acaggatgaa gaaaatttga agcccgaacg
     8281 cggggatata ttttgcggag agtgcgcccg tgaatacgcc gaatatcata cccggcatca
     8341 tcgcaaatac ggttttccag tcgaccgcct gttttttgtg ctgccccaac atactggaaa
     8401 aggcggtgaa gaccatgacg gcgaaggatg tgccgacggc gaggtgttgc gcgtaaggat
     8461 gttgtgccaa accctgcaaa tcaagtaccc ataaaacgac agggacaatc agcgtgccgc
     8521 cgcctacgcc gaacagaccg gcaataaaac ctgccgcact gcctacggca agcaggatta
     8581 agataatgtc ccaatgccac atttaatccg cctttctgta ttttttccag aatcggcgtg
     8641 ccgccataat gtagccggac aggctgtatc cgagaaagaa caggaagagg acgagcgacg
     8701 gttcccaagt gaccagaagc agcaataaga cggcaagcag cattccgaca aaggggactt
     8761 gtctgcggat gttgatttct ttaaaactcc aaaaagggat ttggacaatc atcgacaggc
     8821 cggcaaacag tgtgatgccc aatgcccacc agtggacggc ggggaatttt tcgacgctgt
     8881 ggttgaccca aatcagcccg acaatcagcg cggcggcagt cggactgggc acgccgataa
     8941 accagcgttt gtccaccttg ccgatgagcg tgttgaacag cgcaaggcgc agggcggcgc
     9001 aggcgcagta gatgaaggcg acggaataac cgattttgcc gaactgccaa agctgccatt
     9061 tgtaggcaat cagagcggga gcaacgccga agctgaccat atcggcaagg ctgtcgagct
     9121 gctccccgaa cgcgctttgg ctgttggtca gccgcgccac gcgcccgtcc ataccgtcca
     9181 gcaacataga gatgaatacc gcgatggcgg cggtttcata acgtccgtgc atggattggg
     9241 tgattgcgta aaatgcggaa aacagcgcgg cgatggtaaa ggagttgggc agcaggtaga
     9301 tactgttttg acgcagggag cgttgcgggg tgttggtcgg ggtttccata gctgaagtgt
     9361 ctcacggacg tggagaatcg ataggggatt ataacccaaa ccattatcga acaatacgcc
     9421 ctttaggatg aagccctcgg aggcggtttg tttttctgat ggcggatgct ttgcggggcg
     9481 gtttgactct gcctgccacc gggtgtgttg tttcggattt ttgccggatg gatattgttg
     9541 ccgggcattt ctgggcttgc cggaaggtat cggggggagg gcatcggaat atgcggcgca
     9601 ccgttcaaac cggggcggcg tttccctatt ttcagggggt tgcaggtttc aacaaattga
     9661 aagatatttt ttgcatttcc gaccgtatgc tgattttgaa gcgggataaa ttataggaca
     9721 aggtttttcg cccgccgcaa aggttggccg caatgccgtc tgaaaacaac ggcttgccgc
     9781 taaggcggcg gtatgcggat ttgcgctgcc gaaatcggtt tgccataccc ggcggtgcaa
     9841 tctgcgggga gggataagcc gataaggcgg gacttatcct gttttttgtt tataatgcaa
     9901 cagaatttgg acgaaccgtc gggaatttat catgatgaaa aagatactgg ccgtatcggc
     9961 actatgcctg atgactgcgg cggcacgggc tgccgatact tacggctatc tcgccgtttg
    10021 gcagaatccg cagaatgcgg acgatgtttt gcaggttaaa actacaaaag aagattcgac
    10081 gaaaagcgaa gcgtttgccg agttggaagc cttttgcaaa ggtcaggaca cgcttgcggg
    10141 cattgccgaa gacgagccga ccggatgccg gtcggtcgtg tcgctgaaca atacctgtgt
    10201 cgcgctggca tacccgaaag ccttgggcgc gatgcgcgtt gaaaacgccg ttgtgattac
    10261 ttctccgcgt tttacgagcg ttcatcaggt cgcactcaac cagtgcatca aaaaatacgg
    10321 cgcacaggga caatgcggct tggaaacagt gtattgcacg tcttcttctt attacggcgg
    10381 aactgtgcgc tctttgattc aaaatctcaa ataaaacgga aaatgccgtc tgaaagatgt
    10441 tcagacggca tttccatatc gacggtcagg attctttcgg atcgggcagc aggctgttca
    10501 acacaatggc aagtacggcg cacaagccca cgccggcaaa gctgaagctg cccaatttga
    10561 gcgtcatgcc gccgatgccc gtggtcagta ccgagctgac gatgaccagg tttttcggca
    10621 gcatcaaatc gactttggca tcaatcagcg ttttcacgcc caaagaagca atcgtgccga
    10681 acagcagcag cataatgcca cccatcacgg gcatcggaat ggaagccaaa aacgcattga
    10741 atttgccgaa aaacgccatg cagacggcaa aaaccgccgc ccaagtcatg atgacagggt
    10801 tactgttttt ggtaatcatc accgcacccg ttacttcgcc gtaggtcgta accggcgggc
    10861 cgccgatcag accggcaacg cacacgccca aaccgtcgcc cgcaagggtt ttgtccaagc
    10921 ccgggtcttt cgtatagtct ttccccgtca cattgccgat tgccatgatg ccgccgatgt
    10981 gttcgatggc gggggcgacg gcaacgggca gcataaacag tgcagcctgc cagttgatct
    11041 gaggcgtttc aaaatgggga acggcgaacc agggcgcgtg tgcaatgctt gccgtgtcca
    11101 ccagtcccat cagcagtgcc aaaacataac ccgaagcgac accgatcaag atgggaatca
    11161 gcttcatcat cctgctgccg aaaaccgata cgatggcggt aacggcaaag gtaaagccgg
    11221 aaagaatcag cgaatcggta tagtcgatga cctgtttgcc gtccgcctga cccattgcca
    11281 tgctgcttgc cgccacggca acagacagac cgatgaccat gatgacgggg ccgatgacga
    11341 ccggaggcag cagtttgtgt accgctgcca gtccgcgcca acggatcagc gcggcaaaca
    11401 caaaatacat aaagccggcg gcaaacagtc cgaacatggt ggaaggcagc ccccattcgc
    11461 cgacggagta gataatcggt gcgataaagg caaacgaaga accaagaaaa atcggcactt
    11521 tgcgtttggt tgtgatttgg aacagcagcg ttcccaagcc tgcgcccaaa agcgcaagag
    11581 ccggattcag accggtcagc aggggaacca gcaccattgc gccgaatgcc acaaataaaa
    11641 tctgcgcgcc ggaaacggca agtttcagtt ggttcatacc atcttctttc aatcggttca
    11701 aacggcacgg attatactgg tttgccgagg gtctgcgtaa aaatacatat ggcgggcaac
    11761 agatatgccg tctgaaaggc aatgtctgtc tgttgcagga tgaaagcagg agaaatggag
    11821 tataatccga cactttccat attcaatccg caagccgtcc ggcttcctta tttcagacgg
    11881 cattttccgg taatacggca tgacagaaaa atcccataaa aaaacagcaa aaggcagggc
    11941 gggaagcccg tccccaacgt ccgcacgcaa caagaaagcc gacaacggcg cacggggcaa
    12001 taaagtttcc gagcggctca aagcggtcaa agagttgcag aaaaccgaaa ccaaaaaggc
    12061 gcgtcccgaa catgtcgtca accttatcgg cgacgcactg tggctgatgg gtttggcggc
    12121 aaccttgtat ttggcgattt ccctgatcag tttcgatatg ggcgatccgt cttggtcgca
    12181 cagttcgccg gttgtggaag atgtcgccaa ttggggcgga ctgttcggcg cgtatgttgc
    12241 cgatgtcggc tattatcttt tcggctggtc gttctggtgg tggatagcgg ctgcctgcgt
    12301 catgctgtat aaaaattttc gtctgcacgc aaaacagacg gaaaacgagg catacaacca
    12361 caaaatcgct gccgccgcgc tgtttgtcct gacggtcttc agccccgtct tggagtattt
    12421 tgtgctgggc ggaaaatatg ccgactccct gcctgtcgga gcaggcggta tggtcggcat
    12481 acgcgtcggc gcagtgtttg cgtggctgct ggggaaatcg ggcagcctgc tgattatctt
    12541 ggttgttctg ctgttgtcgt tgtccctgct ggtgcagatt tcatggctgg aatttttgaa
    12601 cggtgcgggc agggcggttc aaaaccgcct gagtgcctta tccggcaagg tcatggcttt
    12661 aggaaaacgc cggccgaata gcaaaacaga cggtgtcgat acccaaaata cacggcgcat
    12721 ggtaaaagaa gccaagaata ttacggccaa acccgttgcc ttgcccgaag gcagcagcag
    12781 caaccgcaaa tccgtcgcgg tttccgtcgc gccgtcgccc aaaattcagg tttctctgtt
    12841 tgaagatgac gaacctcggc aggcgggcga ataccacaag cctacattga acctattgcg
    12901 gattcctgac agcgaacccg tcagcatcaa tcccgccgaa ttggagcgca ctgccgaact
    12961 gatcgaatcc aaactggcag aattcggcat cggcgtacaa gtcgtatccg ccacatccgg
    13021 tcccgtcatc acgcgctacg aaatcgaacc cgcgcaaggt gttaaaggca gccaaattgt
    13081 tgccttgtcg aaagatttgg cacgctctat gtcgctgcaa tccgtgcgta tcgtcgaaac
    13141 catcgcaggt aaaaacacga tgggcatcga gttgcccaac gacaaacgcc aagacgtgat
    13201 gttgagcgaa atcttgtcct cgcccgtgtt tgccgaagcc aaatccaagc tgaccgtcgc
    13261 gctgggcaaa gacattgccg gtacccccgt tgtcggcgac ttggcgaaaa tgccgcacct
    13321 tttggtcgcc ggtatgactg gttcgggcaa gtccgtcggc gtgaacggca tgattatgtc
    13381 tatgcttttc aaagctacgc ccgacgaagt ccgctttatc atgatagacc cgaaaatgct
    13441 tgagttgagc atttacgacg gcattccgca cctgctctgt cccgtcgtga ccgatatgcg
    13501 cgaagcgggg caggcgttga actggtgcgt cgccgaaatg gaaaaacgct accgcctgct
    13561 ttcccacgcc ggtgtacgca atctggaagg cttcaaccaa aaagtcgaag ccgcaaaagc
    13621 ggcaggcaag ccgctgctca atccgttcag tctgaacccc gacagccccg agcctttgga
    13681 aaaactgccg atgattgtgg tcgttatcga cgaacttgcc gacctgatga tgaccgaacg
    13741 caaagccgtc gagcagcaaa tcgcccgtct cgcccaaaaa gcgcgcgccg ccggtatcca
    13801 tatgattgtc gccacccaac gtcccagtgt cgatgtcgtt actggcctga ttaaagccaa
    13861 catcccgacg cgtatggcgt ttaccgtgca aagcaaaatc gacagccgta ccatcctcga
    13921 ccaaatgggc gcggacgaac tgctcaaata tggcgattcg ctgttcctcc agcccggcag
    13981 tgccgaaccg acccgcctgc aaggcgcgtt tgtttcagac gacgaagtac atcaagtcgt
    14041 caactacgtc aaatcgcaag ccccagccga ctatattgaa ggtctgctca gcggcgaggc
    14101 cgcgctggaa actgccaata tcgttaatcc gaatgcagac agcgacgaat tgttcgatca
    14161 ggcagtcgcc tatgttttgg aaagcaaaaa aacctccatt tcgtctttgc agcggcagtt
    14221 gcgtatcggc tataaccgcg cggcaaacct gatggaggca ctggaaaatg cgggtgtcgt
    14281 ttctcccacc gacctcaacg gcagccgtaa aattttggcg cacaaggacc atttgtagcc
    14341 cgtattgcaa aatgccgtct gaacggcgga attggcgttt cagacggcat attatgtttc
    14401 aggcggaaca ttgtgatata cttgccagct aaaatctccc ctttgcggca atgcggttca
    14461 aatatccgta ccgttgcgct gtttgcttcc ccatgcaggg aagaaggttt accattttat
    14521 caacacaaca aatttaaggg cttatgatga gcgtaactgt tgaaacttta gaaaatctgg
    14581 aacgcaaagt agtgttgtcc ctgccttggt ccgaaatcaa cgcagaaacc gataaaaaac
    14641 tgaaacaaac ccagcgccgt gcaaaaatcg acggtttccg cccgggtaaa gcacctttaa
    14701 aaatgattgc ccaaatgtac ggtgcgagcg cgcaaaacga cgtgatcaac gagctggtgc
    14761 aacgccgctt ccacgatgtt gccgttgccc aagagttgaa agtggcaggc ttcccccgtt
    14821 ttgaaggcgt tgaagaacaa gacgataaag aatctttcaa agttgccgcc atttttgaag
    14881 tgttccccga agtcgttatc ggcgatttgt ctgcacaaga agttgaaaaa gtaaccgctt
    14941 ccgtcggtga tgccgaagtg gaccaaaccg tagaaatcct gcgcaaacaa cgcacccgct
    15001 tcaaccatgt cgaacgcgaa gcccgaaacg gcgaccgcgt catcattgac tttgaaggca
    15061 aaatcgacgg cgaacctttt gccggcggcg catccaaaaa ctacgccttc gtattgggcg
    15121 caagtcaaat gctgcctgaa tttgaagccg gcgtagtcgg catgaaggct ggcgaaagta
    15181 aagacgttac cgtcaacttc cctgaagaat accacggcaa agacgttgcc ggtaaaactg
    15241 ccgtgttcac cattacgctg aacaacgttt ccgaagcgac tctgcctgaa gtcgatgcag
    15301 attttgcaaa agccttgggt attgcggatg gcgatgttgc caaaatgcgt gaagaagtga
    15361 agaaaaacgt aagccgcgaa gtggaacgcc gcgtgaacga acaaaccaaa gaatccgtaa
    15421 tgaacgcgct gctcaaagcc gtagagttga aagttccggt tgctttggtc aatgaagaag
    15481 ccgcccgttt ggcaaacgag atgaaacaaa acttcgtcaa ccaaggcatg gccgatgccg
    15541 cgaacttgga tttgcctttg gatatgttca aagaacaagc cgaacgccgc gtgtctttgg
    15601 gtctgatttt ggccaaactg gttgatgaaa acaaactgga accgactgag gagcaaatca
    15661 aagccgttgt cgccaacttc gcagaaagct acgaagatcc tcaagaagta atcgactggt
    15721 actacgcaga gccttcccgc ctgcaagtcc cgacttcttt ggcgatagaa agcaacgtcg
    15781 ttgatttcgt tttgggtaaa gccaaagtaa acgaaaaagc tttgtctttt gacgaagtga
    15841 tgggcgcgca agcctgatta tcctgaaaat gccgtcttag ggcagttttt gaaagcaccg
    15901 aagccgcttt taaagcccgc tttggtgctt ttacatcatg aaaggagacg aaatgtcttt
    15961 tgataactat cttgtcccta ccgttatcga gcagagcggt cgcggtgagc gtgcattcga
    16021 tatctattcc cggcttttga aagagcgcat cgtattcttg gtcggcccgg taaccgacga
    16081 gtccgccaat ctggtggttg cccaactgtt gtttttggaa agtgagaatc cggataagga
    16141 tattttcttc tatattaact cgccgggcgg ttcggtaacg gctggtatgt cgatttacga
    16201 caccatgaat ttcatcaagc ccgatgtatc gactttgtgc ttggggcagg cagcaagtat
    16261 gggcgcgttc ttattgtcag caggcgaaaa aggcaaacgt ttcgccctgc ccaacagccg
    16321 gattatgatt caccagcctt taatcagcgg tggtttgggt ggtcaggcat ccgatattga
    16381 aattcacgca cgcgaactgt tgaaaatcaa agaaaaactt aaccgcctga tggcgaaaca
    16441 ttgcggccgc gatttggcag atttggagcg cgacaccgac cgtgataatt tcatgtccgc
    16501 cgaagaggct aaagaatatg gtctgattga tcaaatcttg gagaaccgcg cttctttgca
    16561 gctttaataa gaaacctgag agagacaaat gccgtctgaa catttcagac ggcatttatt
    16621 ttatagagac tgtatcggga tgacttttcc tttattgcgt ttagaactgt tttcgccata
    16681 attggtttta acatattcct ctacaatagc taggaattcg cttgcaatat tatcaccttt
    16741 cagtgttatt ttgcgctccc catctacata aaccggcgcg acgggtgttt cccccgtacc
    16801 gggcaggctg atgccgatgt cggccaattt gctttctccg ggcccattga caacgcagcc
    16861 cattacggca acgttcaggg attcaacccc gggataaagg gtacgccata tagacatttt
    16921 ttggcgcagg taattttgaa catcttgtgc cagctcttga aatacggtac tggtggtacg
    16981 cccgcagccg gggcaggcgg taaccatcgg cgtaaacgaa cgcaatccca tagtctgtaa
    17041 aatctcttgc ccgacgacga cctcctgagt acgcgggctg ccaggttccg gagtcagtga
    17101 aatgcggatg gtgtcgccga ttccttcttg aagcaagacg gataatgccg ccgttgatgc
    17161 gacaatgcct ttgctgccca taccggcttc ggtcaaaccc aaatgcagcg gataggcgca
    17221 acggctgccc agttcgcggt aaacctgaat caaatcctga accgcgctga ctttgcacga
    17281 caggatgatt ttgtcttcgg gcagtcccaa tagaacggct ttttcggcag attccaaagc
    17341 ggagacaatc agtgcttcct tcgtcacttc ttcgggcggt ttcggcgcgg aagaagcgag
    17401 gttggcatcc atcatacgtt tggcgaggct ttgatccaaa gaaccccagt ttacgccgat
    17461 gcggacggct ttatcgtttt cagcagcagt ccgaatcata aaggcaaatt tttcatcgcc
    17521 ttttacgcct ttgccgacat tgccgggatt gatgcggtat ttggacaatg ctttgccgca
    17581 ttctggaaat tccgccaaca ggcgttcgcc gttgaagtgg aaatcgccga taagcggtgt
    17641 ggcatagccc atatcgtcca agcggcggcg gatttcggca actttggacg cggcttcggg
    17701 gctgttgacg gtaatacgca ccatttcgga tccggcatcg ctcaattcct taatctgcaa
    17761 tgcggtggct tttgcatcgg cagtgtcggt gttggtcata gattggataa cgacgggtgc
    17821 ttctgaaccg acggtaatat gatcgatgcg gacttgatgc gtcttgcggc gttggagtgt
    17881 gttcatatgg tttgaatctg tttattgacc ggtgaggacg gtttgcaatt cttccgagta
    17941 ggggaaattc gcctgcaatt gtgcttcata ttcgtatgcc gcctgtgcgt tgccgagggc
    18001 tttggcaatt ttccagccta gcagcaaatc atcggcctga aggacttcta ccctgctttg
    18061 gtatttttta aagtagtaat cggcatcgcc caactgcccg gccagcattt tggtgcgcgc
    18121 cagttcttta aatgcgggtg ggaactgcgg ctgggcggcg agggaacgtt tcaaataggc
    18181 ttccgccaat ccgaattgcc cctgttttgc gctgcatatg cctttattca ggttggcaat
    18241 ataaggggtc gggtaggtgg ggtcggccag ggctttgtcg aaatatgcca tagattcggc
    18301 agggcggttg agcctgccgc acaggaacca gccgtagttg ttgttgattt cggcactgtc
    18361 gggtttgatg gagagggctt gccggaaact ttcctgcgcc ttgtcgttaa ctttcaggta
    18421 ttgatagatt tcggcacgga ccagccaggc aagctcgttt ttagggtctg atttcaaggc
    18481 gtcttcaata cttgccgtcg cctgacggta gtcctgaccg cgcatatatt ccattgccaa
    18541 ctgggttttg atattggaaa cctgattggc tttttctgcc cgcgaggggc ggtaggaagt
    18601 gctgcacgcg cccaaggcaa ggacaagtaa taaagagatt cgtttggatg gcttaaaagg
    18661 cataattacc cctgttgtcc gattaaaatc tgctgccatt tttgttggcg gcgcgtttta
    18721 tcctgaacct gccccgccaa ctgtccgcag gcggcatcga tgtcgtcgcc gcgcgttttt
    18781 cgtacggtaa cgacaaatcc tgcctgctgc aaaatatcgc ggaacacacg gatgttctca
    18841 ttgctggagc gttcgtatcc ggagtttggg aagggattga acggaatcag attgaacttg
    18901 cagggaacat ctgtgaccag ttcgatcagt tcgcgcgcat gttgcgcctt atcgtttatt
    18961 ccgtccaaca tgacgtattc gaaagtgatg aaatccctgg gtgctttgac cagatagcgt
    19021 tggcatgcgg ccatcaattc tttcaaggga tattttttgt tcaacggtac gatttggttg
    19081 cggacttcgt cattggaagc gtggagggaa accgccaaag ccaccggcat gacatcgcgc
    19141 aacctgtcca tttggggaat catacccgaa gtggaaacgg ttacgcggcg gcggctcaaa
    19201 ccgtagccgt ggtcgtccag catgatgctt aaggcggtaa cgacattgtc gaagttcgcc
    19261 atcggctcgc ccatgcccat catgacgacg ttggaaatca cgcgctcgtt tttcggtgta
    19321 acgcccatcg ctttgtttgc ccaccacaat tgcccgatga tttcggcagc agtcaaattg
    19381 cggttgaagc cctgccggcc ggtcgaacaa aatgtacatt ccaaagcgca gccgacttgt
    19441 gaggaaatgc agagcgtgcc gcgatccgat tcggggatga agacggtttc caccccgttg
    19501 cccgtaccga catccaaaag ccattttcga gtgccgtctg aagatttttg agacatcatc
    19561 agcttgggaa tttcgatgcc tgcctgttcg ttcagtttat ggcgcaacga ttttgccaaa
    19621 tcggtcattt cgttaaaatt ttgcgcgccg gattggtgca tccaacgcat aacctgtttg
    19681 gcacggaaag gtttttcgcc catatcggca aaatgtcggg tcagcccttg aaggtcgtag
    19741 ttgagcagat tggttttcat gtgtttgtgt ttttcttaaa tcaaggcttc aaataaaaaa
    19801 ttgcagggca ggcaagtttg atttgaagcc ttttgcaaca gagttttcag acggcatgga
    19861 tatattttat gccgtctgaa gaggagttgt gcggtgtatt gtatcaacgg gggcagattt
    19921 cggtttggct gaaaaagtag gcaatttcca aagcggcatt ttccggagtg tccgagccgt
    19981 gtacggcatt gatgctgacc gaagtggcaa agtccgcgcg tatcgtgcct tcggcggctt
    20041 cggcgggatt ggtcgccccc atcagttcgc ggtttttcag aacggcgttt tcgccttcca
    20101 gtacttgaat cataaccgga ccgccggtca taaattcaac caatccggcg tagaaggggc
    20161 ggtctttatg aaccgcataa aattcttgcg cctctttgag ggtaagctgc ttcattttgg
    20221 cggcaacgat tttcagaccg ttctcctcaa agcggctgta tattttgccg atgacgtttt
    20281 tgccgacggc atcgggtttg atgatggaga tggtacgttc aatcgccatg ctatatcctt
    20341 atttttactt aagaagaatc aaatcggtat tctatcaaaa aaataactta tccgcctgaa
    20401 tccttgggag atagggtcag gtgcggctgt tccaaataaa cttcggactg catttcgacc
    20461 atccggctgg cggtgcgggt aaattcttcg gcaaaatcgc cttccgtata gatatggttg
    20521 acatctaccg cgccggtggc acacagtttg acccggaaat cgtagagtac gtcaatcagc
    20581 caagtcagcc gccgcgcctc cgccttttct tgcggtgaga gttgttccaa acctgaaata
    20641 aaaaccattt cataatgttc ggccaaatac agatagtcgg actgtgagcg ggggccgaag
    20701 cacagtgcgc ggaaatcaaa ccatatggca cggccggact cggctttgtg gggaatctcc
    20761 cgaccgtgga tggtgctgat gccggggttc aaatcggtaa tgcctgtcat ttctttgaac
    20821 agttttgcca gttttgcctc attttcttca ttggcaggcg taaagaaaat ctcggcgggg
    20881 cggagggtac gcagtcggta gtcttcaccg ccgtcaacgt ttaagacggt caggctggac
    20941 tcgatgagcg cgattgtggg aagaaaactg ctccggtttt gaccttgcgg gtagagttcg
    21001 gaaggcgcgt agtttgaagt cgccaccaaa acaacgccct cgttaagcag gttttccagc
    21061 agacggccta aaatcattgc atccgcaata tcgctgacat gaaattcgtc aaaacataat
    21121 acgcgggttt ctttggcaat ctcggcggca acggatttca acgggttgct ttcgcttttc
    21181 agggttttca gcctctggtg gatttctgcc ataaaggcat gaaagtggac gcggcgtttg
    21241 cggcggtaag ggaggcagcc gaaaaaagcg tccatcagaa agcttttgcc gcgtccgacc
    21301 ccgccataga aataaagccc tttggggact tgcggggaac gcaaactcct gcctaaaaaa
    21361 cggtttcttt tgcgtttgaa catcatcaat tcggtccaaa gccgatcgag gtgttcgatg
    21421 gcggctgcct gcgcgtcgtc gcggatgaag ttgggcagtt gtgaggcagc ctgataccag
    21481 gtcagcgggc tgtggttttc aaacggcggg gctttaaaaa gttggtctct attactcata
    21541 tttaaccttg ttatacttct tgcggcggag aaagaaaatg cttaccgtcg gtattataaa
    21601 tgatttcctt caaacttggc gttttccgtg gaaaagaata atgacgcgca caaggggggt
    21661 atgagcaaag gcggcgtttc aatatggtgc agatatgagc tcaaatgcct tttatagt
//
LOCUS       DAWFAN010000014        21636 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWFAN010000014 DAWFAN010000000
VERSION     DAWFAN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21636
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.014"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(48..1229)
                     /gene="odhB"
                     /locus_tag="WGO65_000370"
     CDS             complement(48..1229)
                     /gene="odhB"
                     /locus_tag="WGO65_000370"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ0326721.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAG
                     VRPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(1329..4157)
                     /locus_tag="WGO65_000371"
     CDS             complement(1329..4157)
                     /locus_tag="WGO65_000371"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ0326722.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDTVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVVGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDVLAL"
     gene            complement(4362..5645)
                     /gene="gltA"
                     /locus_tag="WGO65_000372"
     CDS             complement(4362..5645)
                     /gene="gltA"
                     /locus_tag="WGO65_000372"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0326723.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5757..6005)
                     /locus_tag="WGO65_000373"
     CDS             complement(5757..6005)
                     /locus_tag="WGO65_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ0326724.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6009..6716)
                     /locus_tag="WGO65_000374"
     CDS             complement(6009..6716)
                     /locus_tag="WGO65_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ0326725.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6837..8600)
                     /gene="sdhA"
                     /locus_tag="WGO65_000375"
     CDS             complement(6837..8600)
                     /gene="sdhA"
                     /locus_tag="WGO65_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ0326726.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8603..8944)
                     /gene="sdhD"
                     /locus_tag="WGO65_000376"
     CDS             complement(8603..8944)
                     /gene="sdhD"
                     /locus_tag="WGO65_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ0326727.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFALPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8938..9315)
                     /gene="sdhC"
                     /locus_tag="WGO65_000377"
     CDS             complement(8938..9315)
                     /gene="sdhC"
                     /locus_tag="WGO65_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ0326728.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9594..10997)
                     /locus_tag="WGO65_000378"
     CDS             complement(9594..10997)
                     /locus_tag="WGO65_000378"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0326729.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSNNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     ADVEKWPADKRIMGSAKFIDEHTVQIDDHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEAKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11251..11988)
                     /locus_tag="WGO65_000379"
     CDS             complement(11251..11988)
                     /locus_tag="WGO65_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ0326730.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12267..14543)
                     /gene="metE"
                     /locus_tag="WGO65_000380"
     CDS             complement(12267..14543)
                     /gene="metE"
                     /locus_tag="WGO65_000380"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ0326731.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAAGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHANTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEADAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVESLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(14679..15557)
                     /gene="metF"
                     /locus_tag="WGO65_000381"
     CDS             complement(14679..15557)
                     /gene="metF"
                     /locus_tag="WGO65_000381"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ0326732.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15758..16033
                     /locus_tag="WGO65_000382"
     CDS             15758..16033
                     /locus_tag="WGO65_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ0326733.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16033..16158
                     /gene="ykgO"
                     /locus_tag="WGO65_000383"
     CDS             16033..16158
                     /gene="ykgO"
                     /locus_tag="WGO65_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ0326734.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(16266..16466)
                     /locus_tag="WGO65_000384"
     CDS             complement(16266..16466)
                     /locus_tag="WGO65_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326735.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALRDVAKISAWVLLG
                     AAAATLFWYLVMLYFKHIPDSY"
     gene            16626..17765
                     /locus_tag="WGO65_000385"
     CDS             16626..17765
                     /locus_tag="WGO65_000385"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ0326736.1"
                     /translation="MSQNASVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYAGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALTDYLGIGQWAAVVGGSLGGMQA
                     LQWTISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRALQNVKAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            17762..18343
                     /gene="metW"
                     /locus_tag="WGO65_000386"
     CDS             17762..18343
                     /gene="metW"
                     /locus_tag="WGO65_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ0326737.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTNSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            18460..19305
                     /locus_tag="WGO65_000387"
     CDS             18460..19305
                     /locus_tag="WGO65_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ0326738.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(19355..19915)
                     /gene="efp"
                     /locus_tag="WGO65_000388"
     CDS             complement(19355..19915)
                     /gene="efp"
                     /locus_tag="WGO65_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ0326739.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(19958..21106)
                     /gene="earP"
                     /locus_tag="WGO65_000389"
     CDS             complement(19958..21106)
                     /gene="earP"
                     /locus_tag="WGO65_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ0326740.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWRVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGVLRKRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPLTLLLAGAQIIDS
                     LKQNGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETASAHRCLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(21125..21403)
                     /locus_tag="WGO65_000390"
     CDS             complement(21125..21403)
                     /locus_tag="WGO65_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326741.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIVLLVVYLFHYFGAF"
BASE COUNT         5322 a         5493 c         5475 g         5346 t
ORIGIN      
        1 aattcagacg gccttttcat taacaaataa aaggccgtct gaaacgatta cagatccaac
       61 aacaggcggg ctgggtcttc caacgcgtct ttaatggcta ccaaggtcaa tacagcttcg
      121 cggccgtcaa tgatacggtg gtcgtaagac agagccagat acatcattgg acggacaaca
      181 acttggccgt tttcaaccac agcgcgctct ttagtggcgt gcatacccaa aatcgcagat
      241 tgaggcggat tgatgatcgg ggtggacatc atagaaccga aagtaccgcc gttggtaata
      301 ctgaatgtac cgccggtcag atcttcgata gcgattttgc cgtctttggc ttttttcgcg
      361 taatcaacaa ttgcttgttc gatgtcggca atgctcattt ggtcggcatc gcgcaggatt
      421 ggcacaacca aaccgcgtgg gctgccaatc gcgataccga tgtcgaagta gccgtggtac
      481 acaatgtctt tgccgtcaac agaagcattc acaaccgggt attttttcag ggctgcaaca
      541 gcggctttaa cgaagaagga cataaagccc agtttcacgc cgtgttcttt ctcgaatttt
      601 tctttgtact tcgcacgcaa gtccatgatt ggtttcatgt tgacttcgtt gaatgtagtc
      661 agaatggcgt tttcttgttg agaagccaag aggcgttctg caacacgggc acgcaggcgg
      721 ctcatcggta cgcgttcttc aggacgtacg ccggcaggaa gtgcaacggc aggagcagcg
      781 gctgcggcag gtttggctgc ggcattttgt acgtcttctt tcaatacgcg accgtcacgg
      841 ccggaacctt gcaatgcgtt cacgtcaaca ccgctctctg cagccagttt ggcggcggca
      901 ggcatagcgg cgttgttttg tgcagcagca ggagcggcgg ccggggcagc ttcggcagga
      961 gcggcggcag gtgcttcagc agcggcagta gcagcggtat cgatacgtgc caaaacttgg
     1021 tcggcaacaa cggtttcacc gtcttgcgct acgatttcaa ccaatacgcc ggcttgtgga
     1081 gaaggtactt ccaaaaccac tttgtccgtt tcgatatcga tcaggatttc gtcacgggca
     1141 actgcttcgc caactttttt cttccattcc aagagcgtgc cttcagatac gctttcagac
     1201 agcataggta cttttacatc aataatcatt ttgtgtctcc aatggccctt tcagacggcc
     1261 tgttgtgtgt ttttcattct gcatatgggc attccgcctg agcagcctca gacggaatgc
     1321 tgcttggttt acaacgccaa aacgtcttca accaattgtt tcaattgagc aatgtgtttg
     1381 cttgagtagc ccactgcagg cgatgcgctg cttggacgac cggcataaga cagtttttgc
     1441 tcttcgctga taacgtcttc gatgcggtgg cggatttggt agaacgcgcc ttggtttttc
     1501 ggctcttctt gcgcccaaac cacagatttt gcgttcggat atttcgccag ttcggcttta
     1561 acctcatcgt atgggaacgg atacagctgc tcaacgcgga caatcgcaac atcatcttcc
     1621 agtttacgtt cggctcgacc ggcttccaag tcatagtaaa cctgaccggc acacaatacc
     1681 acgcgtttca cgctgtcgtt gcttgcgcgt tcggcggtat cgccgacaac cggacggaaa
     1741 gtcgaacctt cggtgaagtt ttccagcggg ctcattgccc ctttgaatcg caacaggcgt
     1801 ttggacatga aaatcactag cggtttgcgg tatgaaccta agacttgacg ttgcaagagg
     1861 tggaacattt gcgacgcttc agatggcatg atgacttgca tattgttctc agaacacagt
     1921 tgcaaccaac gttctacgcg tgcagaagag tgctcagggc cttgaccgtc gtaaccgtgc
     1981 ggcaggatgg tggtcagacc gcacaagcga ccccatttgg tttcgcctga agacaggaat
     2041 tgatcaatcg tcacttgcgc gccgttggcg aaatcgccga attgcgcttc ccaaatggtc
     2101 agcttgtcag gtgcggagca ggcaaagccg tactcgaacg ccatcacggc ctcttcgttc
     2161 aaaatggagt cgataaccag gaactcgccc attccttcgc ccatatggcg cagaggaaca
     2221 taagtaccgt cgtcccattt ttcgcgtttt tgatcgtgca atacggcgtg gcggtgcgag
     2281 aacgtaccgc ggccggagtc ctcaccggag atacgcacgc catgaccttt ggttacgagg
     2341 ctggcgtatg ccaaggtttc ggccataccc caatctatgg cctgtttgcc ggatgccatg
     2401 gctttacgcg cttcaatcac acgttttgca gtcggatgca gggcaaagcc ttccggtacg
     2461 gcggtaaact tctcagtgag acgctcaata tctgcggcag gcaaaccggt ttcgatgtgt
     2521 tcgcgccaat ctttgccttg gtatttgctc cagtcgattt gcgtgcgttg aaagttgctc
     2581 aacgttgttt gctcgacgtg ttcgcctttg tccaaagcat cacggtaggc ttggatgtaa
     2641 ccgtctgcct cggcttgggt taccacgcct tcggcaatca gttgctcggt gtacaaagca
     2701 cgcgcaccgg ggtgttgcga tacttttttg tacatcatcg gttgggtcaa ggtcggatca
     2761 tcgccctcgt tgtgacccca tttacggtag cagacaacgt caatcacgat gtctttatgg
     2821 aattttttgc ggtaatccaa agcggcttgg atggcaaagc aaacacgctc aggatcatcg
     2881 ccgttcacat ggataaccgg agcggaaacc atttttgcga tatcggtaca gtgtacggtt
     2941 gaacgggtat cgcggatatc ggaagtggta aagccgattt gattgttgat gacgatatga
     3001 accgtaccgc cggtggtata accgcgcgtt ttagacaggt tgaatgtcgc ttggttgaca
     3061 cccaaaccga taaatgcgga gtcgccgtga atcaatacgg gcaagacttt atcgcggccg
     3121 ttttcgccca aacgtttttg cttggcgcgt gcagaacctt ccactaccgg attgacgatt
     3181 tccaagtgtg acgggttgaa cgccaaagaa acgtgcatcg gaccatgtgg agtggcgatg
     3241 tcggagctga agcccatgtg gtatttcacg tcgccgctag gcaatttgat ttcggcacgg
     3301 ccttcaaact cagcgaacaa atctccgggt tttttaccca aaatgttcac caaaacattc
     3361 agacggccac ggtgcgccat accgatgatg acttcttcca cgccgtcttt accggcgttt
     3421 tgaatcaggt agttcaaacc ggcaatcgcg ctttcgccgc cttcgacacc gaaacgtttc
     3481 tgaccgacat atttggtatg cagataacgt tccaaagtct cggcagcagt catctctttc
     3541 aagatacggc gtttttgatc ggcattgtaa tgcggtgtgg acaatacgct ttcaaaataa
     3601 ttgcgtaccc agcggcgctc ttcggtattg ggaatataga tatattccaa tgcgatgtgg
     3661 ccgcagtagg tttgtttgag gttgctgatg atttgggaca aaggcagttt gccgcgattg
     3721 gcaaaatcgc cctcgcccat attgaattga agcgccatat cggcatcaga cagaccgtgg
     3781 aatttcggat cgagggcttc aatatcgcgc ggggggatac gtttgagcgg atcaagttgg
     3841 gctgcaccca cgccttggat acgataggca gaaatcagcc gtaaaacgct gacttgcttt
     3901 ttcagcattg cctcatccgc accgcccgca acggcagatg caattttctt tttcgccaaa
     3961 gtaacaaatg attcgcgaat cggtgtgtgt gcgacatcga cagcaaccgt ccccggctgt
     4021 ttgctcaaat cggtgaaata ctgcttccat ttttcatcaa ccgtatcggg gttttccaaa
     4081 aaagcctcgt acaattcctc aatgtagggt gcgttcgaac cgaacaggta agagaaattg
     4141 agtttttcgt ccatcatggc gtgcgctctt ttctttaaaa tataaacaaa gggtagaagt
     4201 ccgcgtcctg acggctaaac tgatgccgtc tgaaaagaca ttttattcta ccctatttta
     4261 aaccggattg cttgtcttga tttcatccga ctacaatcag acggattcgg gaaacaatcc
     4321 ggttctctga tattgcctgt ttgacaatat attaatgtat gttacctctc gcctggcggc
     4381 acataatcgc ggcgttccga accggtataa agctggcgcg ggcggccgat tttcagcgaa
     4441 ggatcgctaa tcatctcgtg ccagtgcgaa atccagccca cgctgcgcga cagggcgaag
     4501 atgacggtaa acatttcggt cgggatgccc agcgcggaca ggacgatgcc ggaatagaaa
     4561 tcgacgtttg gatacagttt gcgttcgata aagaacgggt ctttcagcgc aatctgttcc
     4621 aattccatcg ccagtttgaa tttcggactg tcttccaagc ccaattcctt caaaacttca
     4681 tagcaggttt cgcgcataat gctggcacgc ggatccatat tgcggtacac gcggtgaccg
     4741 aagcccatca gacggtattt gcgttgtttc acaccttcca tgtatgcggc aacattagac
     4801 acatcgccga tttcgtccaa cattttcaac acggcttcgt tcgcaccgcc gtgcgaagca
     4861 ccccacaggc aggcgatacc ggcagcaata caggcaaacg gattcgcacc cgaagagcct
     4921 gccagacgga cggttgaagt tgaggcgttt tgctcgtggt cggcatgcaa aataaagatg
     4981 cggtcgagcg cgcgtgccaa aacgggattg ggtttgtagt cttcacacgg cgtggcgaac
     5041 atcatatgaa ggaagttttc ggaataagaa agattattct tcggataatt gaacggcaga
     5101 ccgtttgaat agcggtagca cattgccgca atggtcggga ttttagaaat caggcggtaa
     5161 atcgcgattt tgcggtgttc gggattgcta atgtccaagc tgtcttggta gaacgcagac
     5221 agtgcgccga ccacgccgac catcatcgcc atcggatgcg cgtcgcggcg gaagccccgg
     5281 aagaaccaag tcagctgttc atgcaccatc gtgtggcggc ggacggtatt gtcaaattct
     5341 gccttttgct cgggagtcgg cagttcgccg taaatcaaca ggtagcagac ttccaaataa
     5401 tcggactttt cggccagctg ctcgatgggg tatccgcgat aataaagcaa gccttgatcg
     5461 ccgtcgatgt aagtaatttt agactcacag cttgcggttg aaacaaatcc ggggtcgaag
     5521 gaaaacaaac ctgtattttt tgtcagcccc cgaatgtcaa ccacatcgtg cccgatgctg
     5581 gcttccaata ccggcagctc caaacctgcc tgacccggta cgttgagttt gattgatttg
     5641 gacatattat tgctcctttg caagttataa cccacgttac tgcatccgca gcacgttctt
     5701 tgccttcaga cggcttttgc attttgaaat ctgcctgcag gcagatttca gatgcctcat
     5761 gcccgtctga ttttttcaag catcgggata aggtgccctt tgtccgtttc cgaatgcccg
     5821 ttaatcaagg caagcaattc ttgatcttga aattcaagga tttcggaaaa ctcggacagc
     5881 tctttatcgc tcaaatgctc gaattctttt tccataaacc tgccgaagat taaatctaac
     5941 tccaacaatc cccggcgggt ttgaaaacgg attttccgtt tggcaatatc gtcaaaaacc
     6001 atcatttctt aaacggcccg tttcaacata atctctttaa tcttaccgat ggctcgggtc
     6061 ggattcaagt gtttgggaca tacgtctacg cagttcataa tggtgtggca acggaacaaa
     6121 cggtatgggt cgttcagatt atccaaacgc tcattagtga tggtatctcg gctgtccgca
     6181 atgaagcggt aagcattcag caaaccggac ggaccaacga atttatcagg gttccaccaa
     6241 aatgacgggc aggcagtcga acagcaggcg cacaaaatac actcgtacaa accgtccaac
     6301 tctttacgct cttcctgagt ttgcagacgc tctttgtccg catcaatcgg attatcgttg
     6361 acaacataag gtttgacgga atggtattgt ttgaagaact gggtcatatc cacaatcagg
     6421 tcacggataa caggcagacc tggcagagga cgaattttaa ccggctgttt caagccacgc
     6481 agatcggtca aacacgccaa gccgtttttg ccgttgatgt tcataccgtc agaaccgcaa
     6541 atgccttcgc ggcaggagcg gcggaaagac aaggtatcgt cttgtgcttt caggcgtacc
     6601 aaagcatcca aaagtttcac gtcggtcggt tccaattcca actcgtaacg ctgcatataa
     6661 ggcttggcat caacatccgg gttgtaacgg taaatttcaa aactcatttt ttccatgaga
     6721 agtgttcctt ttctcatcaa ctgcccaatg attttgtatt ataaattcaa gcagttcaat
     6781 gtgggtatgg ttagatttca gacgagctta tgaggcgaaa accgtctgaa aacgcatcaa
     6841 taaacgcgct tggccggttt gatgtattcc acgctcaaag gcttggtatg taccggtttg
     6901 taagacaaag tattggtatc tgaatggtac agcgtatgtt tcatccagtt ttcatcatcg
     6961 cgctcaggat ggtcgtctga agcgtgcgca ccgcgtgatt ctttacgtgc ttcggcagac
     7021 accaaagtcg ctttcgccac ttcaattagg ttatccaatt ccaaagcctc gatacgcgcg
     7081 gtattccaca ctttgctctt gtctttgatt tcggtacgtt tcacacgctc ggcaatcgcc
     7141 atgacttctc gaacgccttt gctcagaatc tcatcagtac ggaacacgcc ggcgtgcagt
     7201 tgtacggagc gttgcaattc gcgacgcaat gcatcaacgt tttcgccatc agtttgattg
     7261 tccaaacgct cgatacgttg gcgggtcagc tcgcccgcat ccgcaggcaa aggtttccag
     7321 tcgctttgct ctttgatgaa tttaatcatg ctgtcgccgg cagctttacc gaataccacc
     7381 aagtccaaca gggagttggt acccaagcgg ttcgcaccgt gtacggaagc acaagcgcac
     7441 tcacctgccg catacagacc ttttacaggc acttcgtatt cgtcgccttg aggaacgaca
     7501 acttcgccat ggtagttggt cggaataccg cccatcatat agtgggtagt cggcacaacg
     7561 ggaatcgggt ctttaatcgg atcgataccg gcgaactgaa tggaaatctc gcggatgccc
     7621 ggcagttttt ccataatttt ttctgcgccg atatggtcga ttttcagtaa gacatggtct
     7681 ttgtttttac cgcagccgcg accttcgtag atttccatcg ccatcgcgcg ggaaacaacg
     7741 tcgcgagaag ccaagtcttt tacggtcggc gcatagcgtt ccataaagcg ttcgccgtcg
     7801 gcattcaaca gaataccgcc ctcgccgcgt acgccttcgg taatcaacac gcccgcacct
     7861 gccacgccgg tcgggtggaa ttgccagaat tccatgtctt ccaacgggat acctgcacgc
     7921 gcacaaatac ccaaaccatc gccggtattc atataggcat tggtagaaga cgcataaata
     7981 cggccgccgc cgccggtagc aaacatcaca gctttagcgt ggaaaatata aacttcgccg
     8041 gtttccattt ccatggcggt tacgccgacg acatcgccgt tttcatcacg aatcaaatct
     8101 tgtgccgtcc attccacaaa gaattgcgta ttggcacgga cattttgttg gtacaaagta
     8161 tgcagcatcg catgacctgt acggtcggca accgcacagg cgcgttctac cgcgcgttta
     8221 ccgtgttcgg cagtatggcc gccgaaagga cgctgataaa ttttaccgct ttccacacgg
     8281 tcaaaaggca tacccatgtg ttccaactca attacggctt caggcgcggc gcggcacata
     8341 aactcaatcg catcttggtc gcccaaccag tcggaacctt tcacggtatc gtacatgtgc
     8401 cagtcccaac ggtcttcctg cacattaccc agagaggcgg aaataccgcc ctgcgccgct
     8461 acggtatgcg aacgggtcgg gaacacttta gacaaaacgg cacaattcag accggatttg
     8521 gataattgga gggctgcgcg taaacctgca ccaccaccgc cgacaatcac ggcatcaaac
     8581 ttgcgaacag gaaaacccat acttaccccc aaattacttt aattgaatac accaagcagc
     8641 cgaccagcca gacgatggtg gcaacctgca aaaacaaacg cacgccgaag ggtttgatat
     8701 agtccatcca caaatcgcgg atacccaccc aagcgtgcaa gaatacggcg atgaagctca
     8761 cttgggtaaa tacttttacc caagtttgac taaaaaatgc ctgccatgcc gaatattctt
     8821 taggcagagc aaatagaacc actaaaagtg caacggtata aatcaacata ataaccgcgg
     8881 tcgcacgttg catcgcccaa tcccgcaaac cgtaatgggc accggtcaat ttacgttcta
     8941 ccataacaac gctcccaaaa cgacagtcaa aaccaatgca gaagcaaata cggctttagc
     9001 ggtattgcgc gcagtattca gctcaaggcc tttgtgcgca tccaaaaata aaaagcggat
     9061 accggcgaga gaatggtgca gataagccca caatacaccg attaaaacca gcttgaccaa
     9121 aggatgggaa acaatggcac ggtaagtttc aaatgcagac tcttgactca gggtaccgga
     9181 caggaaatac agcaggaaag gcagcataat aaacagcccg accccgctga tgcggtgaag
     9241 gatggaaact atcccgggta tcggcagacg gatgttcggc aaatccagat aaacaggacg
     9301 cggtttggca gacatagtca attcctctgt agttatatat cgactcaaaa accgtaaacc
     9361 cattgctaca ctgggttaca taaacgttaa tttacctgtt ttatcccatt ttgaacagtg
     9421 tattttaaaa acaaactgat agattttgac aaacacgcgt ggaaacagca gattaaaaga
     9481 aagattttta gttttgccgc ccccttcccg acacggtatc agcatccgac cccatcccca
     9541 aaaacaaaat gccgtctgaa aaaatttcag acggcattgt tttgccatat cggtcaggct
     9601 ttcaatttcg catcggcatc gcgcaacgcg gtacgcaaac cttcctcgat aacgggatgg
     9661 tagaacggca tatccagcat ttgcggaacg gtcatcttca tttgatgtgc ccaagccaac
     9721 aggtgcgcca aatgttcggc ggccggacct acgatttctg cgccgatgaa gcggccggtg
     9781 gctttttcgg catacaggcg catatggcct ttgtttacca gcatcacgcg gctgcggcct
     9841 tggtttttga acgatacttc gccgatgaca aattcgtcgg cttggtattg cgcggcaacc
     9901 tgcgcgtatt tcaaaccgac aaagccgatt tgcggactgg tgaacactac gccgatggtg
     9961 ctgcggcgca aaccgccgcc gatattcggg tagcggcctg cgttatcgcc ggcaatcttg
    10021 ccttggtcgg cggcttcatg cagcaggggc agttggttgg acgcgtcgcc cgcaataaag
    10081 atatgcggaa tactggtctg catagtcagc ggatcggcaa cgggtacgcc gcgcgcatct
    10141 ttttcgatat tgatgttttc caaaccgata ttgtcaacgt tcggacggcg gcctacggct
    10201 gccagcatat attcggcaac aaatacgcct ttttcgccat cctgctccca atggacttct
    10261 acattgccgt ctgcgtcgag tttggcctcg gttttagcat ccaaatgcag tttcaattct
    10321 tcaccgaaca cggctttcgc ctcgtctgaa acgacggggt cggaaatgcc gccgatgatg
    10381 ccacccaaac cgaaaatttc aactttcacg cccaaacggt gcaatgcctg acccagttcc
    10441 aaaccgataa cacccggtcc gaacacggca acgcgcttag gcagcgtatc ccatgagaaa
    10501 acgtcatcgt tgataatcaa acgattgccc aaagactgcc attgcggcag gatgacggga
    10561 cgcgaaccgg tagcaatcac gaaacttttt gccgtaattt gggtatggtc gtcgatttgg
    10621 acggtatgct cgtcgataaa cttagccgag cccatgatgc gcttgtcggc aggccacttt
    10681 tccacatcag caacgacaaa gccgacaaaa cggtcgcgct cggatttaac acgccgcatt
    10741 acttcttcac cgttgacgac gatgctgtct ttgtccaaat gcacgccgaa cgggtcggta
    10801 tgcagtgcgt ggtggcgcgc ttctgcggcg gcaatcaaga gtttggaagg catacaaccc
    10861 acccgcgcgc aggtcgtgcc gaacacattg ttttcaatca ggtaaacatt gttcgaatgc
    10921 aaacgggcat tgcgaaacgc gcccataccg gcagtaccgc cgccgattac gacgacatct
    10981 gcttgaattt ttttcataat ctttgtcctt ctttattaag cgtccgaagc agcatcagcg
    11041 tgatgcgtat caggttttca gacggctttt ttaataaact atcgacaata taaaaccaat
    11101 agccatttta aaagtttcga tttaaaacaa taatctcaaa ttctacttgt aaatatagag
    11161 ctattcagaa cgaccgtgta gcgtatcgtc tgaataactc tattttcctg ttcagacggt
    11221 tttattcaaa ccgccttaag caaacagcta tcagttttta gccaagtaag cttccaaatc
    11281 ttcgctgccg ccgatgtatt tgccgccgat gaagacttga ggggcagtca tcttgccggt
    11341 aatggcgcga acggaagtaa cggttgcatc tttgcccaat acgatttctt cgtaagacaa
    11401 acctttgtct tgcaaagctt gtttggcttt agcgcagaat tggcaacctg gtttagtgaa
    11461 aattgccaca gactcttgag ccttccaatc gggagcaacg aattgcagca tagtatctgc
    11521 atcggatact ttgaacggat cgcccggttc ttcaggttcg atgaacattt tttcaaccac
    11581 gccgtcgtta accagcatgg agtaacgcca agagcgttta ccgaagccca agtcttcttt
    11641 accgaccagc atacccatac cttcggtaaa ttcgccgttg ccgtcaggaa tcatgtagat
    11701 gttgtcggat tcttcttcgg cagcccaagc gttcattacg aacgtatcgt ttacagatac
    11761 gcagtagatt gcgtcaacgc cgttttcttt gaacgcgccg aacaattcgt tgtaacgcgg
    11821 caggtgtgaa gaagaacaag tcggggtaaa tgcaccgggc agggagaata cgactacttt
    11881 tttgcctttg aacaaatcat cggtagacac atctttccaa gtgtcgccga cgcgggtgcg
    11941 gaatactacg gaaggtactt tttgaccggt acgatcttgc aaagccattt taagctcctt
    12001 aatagattgg tttgagaaat aattgtgaat cgctgggcat tctaccccat gcaaaatgat
    12061 ttgtataatt tatagttcaa ataatataaa ttatttaaaa ctatcaaaca caactccgat
    12121 ttgcctgttt cccacatata aagccgaatt agaaaaaaac aagctgcaat gaaaagattt
    12181 tccggcaact gcgcccttgc gccgcaaatc aggacaaaat accgtctgaa aggctttcag
    12241 acggtattta ttcatacggt ctcggcttat ttcgccaatt cggcacgcaa tttgtgggtt
    12301 acgttcatca tcacttggag ttgttccaga gtttctttcc agccgcgtgt tttcaggccg
    12361 cagtccgggt taacccacag acgttcaacc ggtacaacct cgatggcttt gcgcaacagg
    12421 tgctccactt cggcttctgt cggtacgcgc gggctgtgga tgtcgtaaac ccccgggccg
    12481 atgtcgttcg ggtatttgaa ttcgccgaac gcggtcaaga gttccatgtc ggaacgtgaa
    12541 gtctcgatgg tgatcacgtc cgcatccatt gcagcaatcg caggcaggat atcgttgaac
    12601 tcggagtagc acatatgagt gtggatttgt gtgctgtctt cgcaaccggc agaggacagg
    12661 cggaaggatt cgcccgccca gttcaggtag gcatcccaat cggcgcgttt cagcggcaag
    12721 ccttcgcgga tggcaggttc gtcaatttgg atgactttga tgcctgcttt ttccagatcc
    12781 aatacttcgt cgttcagagc cagtgcgatt tgtttgcaca cggtagagcg aggaatatcg
    12841 ttgcggacaa aagaccattg cagaatggtt acagggccgg tcaacatacc tttcatcggg
    12901 cgtttggtca ggctttgtgc gtaagtagac caggcgacag tcatggcttc aggacggctt
    12961 acgtcaccga agataatcgg tggtttaaca caacgtgagc cgtagctttg tacccagccg
    13021 tattgggtga atgcaaaacc gctcaacaat tcgccgaagt attcgaccat gtcgttacgc
    13081 tcggcttcgc cgtgtaccag tacgtccaag tccagttttt cttgctcttc aactaccaag
    13141 gcgatttctt ttttcatcgc ggcttcgtaa tcggcagcag acagttcgcc ttttttgaag
    13201 gctgcacgtg cttggcggat ttcggtagtt tgcgggaaag aaccgatgtt ggtagtcggc
    13261 agcagaggca ggttcaacca tgcttgttgc gctttgatac ggtcggcaaa tggagatttg
    13321 cgttggtctg cgttggcagg caaatcggcc aggcgtttgg caacgtctgc acgatggatt
    13381 tcgctgctgt tggcacggga gtcggcagca gcttggctgg cggccagttc ttcggcaaca
    13441 gaatcacggc cttcgttcaa tgcggctttc agaacgcgca attcttgggt tttttgcagg
    13501 gtgaatgcca accaagagta caggtcaggt ttgttggctt tcagtttttc ttcaactgac
    13561 aagtcaaatg gagtgtgcag cagagagcaa gagctggaga tccacaaacg gtcgcccagt
    13621 ttggcttgca gagactcaac agtttccaaa actttgttca ggttggcgcg ccaaatattg
    13681 cggccgtcaa tcacgccggc ggacaggact ttgtcgtagt cggcgaacgc gtccagttgc
    13741 tcgggagcgc gtaccaagtc gatgtgcaga ccgtcaacag gcagggcttt cagcaatgcg
    13801 gcgtgttcgg caacagaacc gaagtaagtg ctcaacagga ttttggcact tactttgttc
    13861 aaagtagcgt aaacgtcttt gtaggcttcc acccattctt taggcaaatc gacagccaaa
    13921 gcaggctcgt cgatttgaat ccactcggca tcggcttcaa ccaaagcagt caggatttca
    13981 acgtaaacag gcaacagttt aggcaacagg ctcagacggt cgaattcgac ggcgcctttt
    14041 tctttaccca cccacaggaa agtcaacgga cctacaacgg tcggtttggc tttcagaccg
    14101 agggcttggg cttcttgcag ttgttggacg tagtgtttgg cgttggcttt gaattcagta
    14161 ttggcgtgga attcaggcac caagtagtgg tagttggtgt cgaaccattt ggtcatttcg
    14221 atagcgaatt ggtctttgtt accgcgcgcc agttggaaga attgttccaa agacaggttt
    14281 tggctgtcga agccgaagcg ggcgggaatc gcgccggtgg cgacttgcag gtcgaggatg
    14341 tggtcgtaga aagtgaaatc gcctgcggca acgtaatcgg cgttggcagc agcttggtgt
    14401 ttccagtttt tctcgcgcaa gtctttagca acagccagca attcttgctc gctgatttct
    14461 ttgcgccagt atttttcttg ggcgaatttc aattcgcgga aggcgccgac acgcgggaag
    14521 cctgagaaat gtaatgttgt catgttgaac tctccagttg gattttgtaa gaaccggttc
    14581 aatccgaatc ggtcggtatt gtaaatcaga atggctcgga tggggcggcg ttttgctttt
    14641 cagacggcct cggatgccgt ctgaagccgt tttcagcttt aagggcgcac gcctaaaata
    14701 tggcagatgg cgtaagtcag ctcgctgcgg ttgagcgtgt agaagtggaa atctttcacg
    14761 ccttcgcggg ataggacttt gaccatatcg atggcgatac tggcggcgac gaggttgcgc
    14821 gtgccttggt cgtcgtccaa accttcatac atttgcgaca gccagcttgg gattttgacg
    14881 ttggtaacct gcgccatttt gccgagctgc ttgaaattgg taacgggcag gatgccgggg
    14941 acgatttcca catcgatgcc caacatcacg cagcggtcgc ggaagcgcag gtagcgttcc
    15001 acatcaaaga aaaattgggt gatgacgtgg ttcgcgcccg catcgatttt gcgtttcaaa
    15061 ttaatcaggt cggcttgtgc ggatttcgct tcgggatgca cttcgggata tgctgctaca
    15121 gagatgtcga agtcggcgac ggagcgtaat agcttaacca agtcttcggc ataaaacggt
    15181 tttttctcgt aaccgggcgg ctcgtcgccg cgcagggcga caatgcggcg gatgccgctg
    15241 tcccaataat ctttggcaat ttggcgcaat tcgtcgggag aagcgtcgat accggtcagg
    15301 tgcggcgcgg cttccaagcc ggtttcttgc ttaatgcgtt tgacgatgcc gtgcgtgcgg
    15361 tcgcgttcgc cggaatttgc gccgtaggtt acggatacga acttgggatg cagggtttgc
    15421 agacggtgga tggaatccca cagcatcgtt tccatttgtt cgtttttcgg cgggaaaaat
    15481 tcaaacgaaa cgttgatgtc gcctttcaaa tcggaaaggc tgttgtttaa cgcattgatt
    15541 tcttttgcgt aattcatggc tgcgtacctt ttattatctt ttatatgata ttttcaggca
    15601 acataataaa gttgcgccaa acatgagtca atttcaaaat tttcagattt atcatgaaaa
    15661 tttctaatat attacaaaaa tagttaaatt tccttgtaca agccttctaa tttatgataa
    15721 agtttgttat gttataacaa ttcaactaag gcgatatatg aaaccgaata ttcatcctga
    15781 caattatcga accgtccttt ttttcgacag cagcgccaat gaaggctggt tgattcgttc
    15841 ttgcgccgaa acacacggaa aaacgatggt ttggacagac ggcaaagaat atccgctgtt
    15901 ttcgctggat acttcttccg catcccatcc cgtctatact ggcaaacagc gcaacgtcaa
    15961 caccgaaggc cgtgccagca aattcaacca acgtttccaa tccgtcatgt catcgtttag
    16021 aaaggacaaa taatgcaggt tctgtcttca ctcaaaaccg ccaaacaacg ccaccgcgac
    16081 tgccaaatcg tcaggcgcag gggcaaagtt tacgtcatct gcaaaagcaa tccgcgtttt
    16141 aaagcacgcc aacgctgact ccctgccctt cctgcttcgg ttgtaaatcc cctcctaccg
    16201 caagcccttt tcatcaaata tgccgtctga aacagaaaaa tgcgtttcag gcggcatttt
    16261 ttccgtcaat acgaatctgg aatgtgtttg aaatacagca tgaccaaata ccaaaacagc
    16321 gtcgcggcgg ctgcaccgag cagcacccac gcgctgattt tggcgacatc gcgcagggcg
    16381 cgtttctgcc tgcggctgaa gaggcggttg ataatcagcg cggcaagtgc gaaaaagaaa
    16441 aaaatacgga aaagtctgcc tatcatatcg ggaagcgggg ttagggctgt ctgaaaaagt
    16501 atggatcggt tgcggcggca tagggacgga tgtttcatcg catcgggctg cttgtccgac
    16561 cgtgtcaaaa agtcatgccg tttgctatat aataacgctt ttcgccgcat ttgttggaac
    16621 aggatatgag tcaaaatgcc tcggtgggca ttgtaacgcc ccaaaaaatt ccgtttgaga
    16681 tgccgctggt tttggaaaac ggtaaaactt tgccgcgttt cgatctgatg attgaaacct
    16741 acggcgagct gaatgccgaa aaaaacaatg cggttttaat ctgccacgcg ctgtcgggca
    16801 accatcatgt tgcgggcagg cattcggcgg aggataaata tgccggctgg tgggacaata
    16861 tggtcggtcc cggcaaaccg attgatacgg aacgtttttt cgtggtcggt ttgaacaatc
    16921 tgggcggctg cgacggcagc agcggacctt tgtcgatcaa tcctgaaacg ggcagggaat
    16981 acggcgcgga ttttccggtg gttacggtga aggactgggt aaaatcccaa gccgcgctta
    17041 ccgattatct cggcatcggg caatgggcgg cggttgtcgg cggcagcttg ggcggtatgc
    17101 aggctttgca gtggacgatt tcctatcccg agcgcgtgcg ccatgcctta gtgattgcgt
    17161 ccgcgccgaa actgtccacg caaaatatcg cgtttaatga tgtagcacgt caggcgattt
    17221 tgaccgatcc cgatttcaac gaaggacatt accgcagccg caacaccgtt cccgctcggg
    17281 gcttgcggat tgcccgcatg atggggcaca tcacctatct tgccgaagac ggtttgggca
    17341 aaaaattcgg acgcgatttg cgttccaacg gctatcaata cggctatggc gttgaatttg
    17401 aagtagaatc ctatctgcgc tatcaaggcg acaaattcgt cgggcggttt gatgccaaca
    17461 cctatttgct gatgaccaag gctttggact atttcgatcc ggcggcggat ttcggcaaca
    17521 gcctgacccg cgccctgcaa aatgtgaaag cgaaattctt tgtcgccagc ttcagcaccg
    17581 actggcgttt cgcacccgaa cgttcgcacg aactggtcaa ggcactgatt gccgcccaaa
    17641 aatccgtgca gtatatcgaa gtcaaatccg cacacgggca cgatgccttt ttaatggaag
    17701 acgaagccta tatgcgtgcg gtcgccgcct atatgaacaa cgtttataag gaatgtcagc
    17761 aatgaacctg cgcgatgatt tgcaactgat ttacgaccgg atacccgaag gcagccgcgt
    17821 cttggacttg ggctgcggcg acggcgaact gcttgccgca ttggtcgaac acaaaaaatg
    17881 cagcggctac ggcatcgaaa tcgacacaaa tagcgtcatc gccgcgatgt cgcgcggcgt
    17941 aaatgttatc caagccgatt tggaagaagg tttaaccgca ttcaacgatc aaagttttga
    18001 tgtgattgtg ttgagccaaa ccatccaagc gatgcagaat accgaaaaaa tcctgcgctg
    18061 cctgatgcgc gtggcaaaac aggcaatcgt cagcttcccg aatttcggct actggcgcaa
    18121 ccgcgtccaa atcgcactcg gcggacatat gccggtttcc gaacgtatgc cctaccattg
    18181 gtatgatacg ccgaacatcc attggtgtac gctcaaagac tttgatttgt tgtgcgccaa
    18241 aaacaacata cgcgtcctcg agcgcgccgt gatgacgggc aacagacagg tcaaacattt
    18301 ccccaacctt ttgggcagcc ttgcgtttta ccgcgtcggg taagacagat tgagaacctt
    18361 gtcggcaata acaggttctt cggttctatt aacaacaggc cgtctgaaaa atgaaatggc
    18421 aggttttcag acggcctctt taatattgga aacaatagaa tgaaaaacaa aacctcatca
    18481 cttctcttat ggcttgccgc aatgatgctg accgcgtgtt ccccgagcaa agaagataaa
    18541 acgaaagaaa acggcgcatc cgccgcctcg tccacggcat ccgccgcttc gtcttccgcg
    18601 ccccaaaccg atttgcaacc ggccgcatcc gcccctgata acgtcaagca ggcagaaagc
    18661 gcgccgccgt caaattgcac cgacctgcac cccgccaccg gcattgacga tctcatgcag
    18721 caaatcgccg aacacatcga ctcggactgt ctgtttgccc tttcccatca cgaactggaa
    18781 acccgtttcg gcttacccgg cggcggctat gacaacatac agcggctgct gtttcccgac
    18841 atccgccctg aagatcccga ctaccatcag aaaatcatac tggcaattga agacttgcgt
    18901 tacggaaagc gcacgatcag ccggcaggca caagatgcct tgatggaaca ggaacgccgc
    18961 ctccgagaag cgacgctgtt gctgatacag ggcagtcaag aaacccgcgg acaaggcgag
    19021 gagccgaaac gcacgcgtta ttttgaagtt tcggcaaccc ctgcctattc gagccggcac
    19081 aacaacggac ttggcggcaa tttccaatac atcggccaat tgcccggcta tctgaaaata
    19141 cacggagaaa tgcttgaaaa ccaatcactc ttccggctgt ccaaccgtga acgcaatccc
    19201 gacaaaccgt ttttagacat ccattttgac gaaaatggca aaatcacgcg tattgtcgtt
    19261 tacgaaaaaa acatctactt caatccaaac ttggggcgaa gataaaaaaa tgccgtctga
    19321 aacgttttca gacggcattt ttcaatgcaa caaatcaagc gcgtttgcgg aattcgccgg
    19381 tgcgggtatc gatttcgatt ttgtcgccgt tttcgatgta agacatcact tggatttcgg
    19441 tgcccccgac caggcgcgcg gttttcatca ctttgcctga agtatcgcct ttgacggcag
    19501 gctcggtata ctcgacttcg cgtacgatga tggtgggcag ttctacggag atggggttgc
    19561 cttcgtagaa ggtaacttcg cattggtctt ccataccgtc aacgatgaat ttcaacgcgt
    19621 cgccgatgtt gtcggcttcg atttcgtatt ggttgaattc ttcgtccata aagacgtaca
    19681 tcggatcggc aaagtagctg tacgtacagt ttttgcggga caggatgacc acgtcgaatt
    19741 tgtcgtcggc tttgtaaatg gtttcggaag ccgcgccggt cagcaggttt ttcagtttca
    19801 tgctgacttt ggcggaagag cggccgcctt tgatgtattc ggttttttga acgaccatag
    19861 gatcgttgcc gaccataaat acattgccgg cgcgcagttc ttgtgctgtt ttcattgtga
    19921 ttttccaatc aaatcggttg tcgtaaaacg cactattcta gcgtattttt tgatgctttg
    19981 aaacaaaggc ggcgagtttt tcggatgcgg aaggctgccc gaaaagataa cggctccaat
    20041 cctccgcgcc ttgccgccag ccgttttgat gttgttgcag gatttgccaa cattcgaggc
    20101 gttgcgtggc ggataaagcc cctccgccgt tgaggtcgtc tgaaaggcag cggtgtgccg
    20161 atgcggtttc gggcgtgtag aaaccgtgtg ccttatccca aaaggcgtgg agtttgtcga
    20221 gatggacatt ctcgtcttgc ggatagatgt gccaaaagaa gggttttccg gcaagctggg
    20281 cgcgcacgaa actgtcttcg ccgcggatga cggcgcagtc ggcaaggtgc agcagtttgt
    20341 cgaagtcctg ttgcggcacg aaagggattt tgacgaggcg gacggatgcc gtctgaaaaa
    20401 catcgccgtc gttttgcagg gcatcttgcg gaataacgcc gttttgtttg aggctgtcga
    20461 taatttgcgc ccccgccagc aacagtgtca acggactgcc tgcctgtcgc cacatttcca
    20521 gccactttgc ccaaacatcg ctccgatagc cgaaaagcag ccattcgggg gcgttttttt
    20581 cgggaagcat cagcctcttg cgcaagactc cgctatcgaa acggacggct tcgcagtaat
    20641 cacgttcgcg tatcaacccg ccgctttttt cgctgaaacc cataaaccaa aaatattttt
    20701 gaacaccctc ctgcggcgaa ggcatcagat gcagcctttc attgctttcc tccgcgctca
    20761 aatattccca attcagccaa agcggcttgt gtcggcggat aatgtgcagc acattttcgg
    20821 gcaggtcgca ggcaaaagtt tcgatgacga catcgggaac aggcgcggta tcaatatctg
    20881 ccgcatcgga atgccaagtg cggacatgaa tatcctgatg aacgcaggga acatcgggca
    20941 aatcagggca aagcgcacgc aaggcggaca catcgtccgt ccacaaatgc acccgccaac
    21001 cgagttcgcg gtgcaaaaca cgggcgagcc gccacgaaac gccgatgtcg ccgaaattgt
    21061 cgatgacctt gcaaaaaatc caacagacaa aaggaggagt attcatagga ataacggaaa
    21121 caagctaaaa cgcgccgaaa tagtggaaca aatacacaac cagcaaaaca atcggaataa
    21181 acagcaacat ggtttgacgt ttcaaatcaa actcttgttt caaatgatgc ttgatggtgt
    21241 cgaaaaggac gaatttgaac aaaaacgtcg cttcgcaggc aaggatgatg ccgttgatca
    21301 gatgctgggg tttgtcttcg ggcggattgg cgacgaaaac ggcaagcgtc gccaaaggga
    21361 tgaggcagac gacaagccag cggacaagca ggtttcggga cattgcaagg ctttcaaaaa
    21421 agcaaaacgc cattatcgcc ccaagcgcac gccttgtcta acggacgcgc cggcgatgcc
    21481 gtctgaagcc ttgccgcagg aaaacaccgc accgccgaac ctttaaatac gcccgagacg
    21541 ggcgttcccc gaaaaatcgg acgctgccgc cgatgcctgc ggacaaggct gcatcgggcg
    21601 cgggcatatt gaaaacccgg cgcgtcggtt tgaaat
//
LOCUS       DAWFAN010000015        21583 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWFAN010000015 DAWFAN010000000
VERSION     DAWFAN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21583
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.015"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(305..4474)
                     /locus_tag="WGO65_000391"
     CDS             complement(305..4474)
                     /locus_tag="WGO65_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ0326742.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMHRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTGKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPSFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSNSFSTSPLGGKLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
     gene            complement(4529..6376)
                     /locus_tag="WGO65_000392"
     CDS             complement(4529..6376)
                     /locus_tag="WGO65_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ0326743.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAS
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6906..8135
                     /gene="sstT"
                     /locus_tag="WGO65_000393"
     CDS             6906..8135
                     /gene="sstT"
                     /locus_tag="WGO65_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ0326744.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIILYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8247..9527
                     /locus_tag="WGO65_000394"
     CDS             8247..9527
                     /locus_tag="WGO65_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0326745.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNAGNTAAQGTNQAENNQVGGSQNPAPSSNPNATNGGNFGRVDLANGVLIDGPSQNI
                     TLTHCKSDSCNGDNLLSEEAPSKSEFEQLSDEDKIKKYKKDGEKFTGLVADRLQMKGT
                     NQYIIFYKPKTTSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLSGGSYALSVQGKPAKGEMLAGTAVYNGEVLHFHTENGRSYP
                     TRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGNWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9603..9818)
                     /locus_tag="WGO65_000395"
     CDS             complement(9603..9818)
                     /locus_tag="WGO65_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326746.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(9811..10026)
                     /locus_tag="WGO65_000396"
     CDS             complement(9811..10026)
                     /locus_tag="WGO65_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ0326747.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10101..11444)
                     /gene="argG"
                     /locus_tag="WGO65_000397"
     CDS             complement(10101..11444)
                     /gene="argG"
                     /locus_tag="WGO65_000397"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ0326748.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRTQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAITGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLALGEGSVLPQLGNKK"
     gene            11771..11911
                     /locus_tag="WGO65_000398"
     CDS             11771..11911
                     /locus_tag="WGO65_000398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326749.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            11908..12708
                     /locus_tag="WGO65_000399"
     CDS             11908..12708
                     /locus_tag="WGO65_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ0326750.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     VTRKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTKRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12748..13734
                     /locus_tag="WGO65_000400"
     CDS             12748..13734
                     /locus_tag="WGO65_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ0326751.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14391..14653
                     /locus_tag="WGO65_000401"
                     /pseudo
     CDS             14391..14653
                     /locus_tag="WGO65_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14691..15065)
                     /locus_tag="WGO65_000402"
                     /pseudo
     CDS             complement(14691..15065)
                     /locus_tag="WGO65_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15238..15594)
                     /locus_tag="WGO65_000403"
     CDS             complement(15238..15594)
                     /locus_tag="WGO65_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ0326752.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     ILEYAIKNEKTVIFHF"
     gene            complement(15737..16082)
                     /locus_tag="WGO65_000404"
                     /pseudo
     CDS             complement(15737..16082)
                     /locus_tag="WGO65_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16241..16641)
                     /locus_tag="WGO65_000405"
                     /pseudo
     CDS             complement(16241..16641)
                     /locus_tag="WGO65_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16645..17385)
                     /locus_tag="WGO65_000406"
                     /pseudo
     CDS             complement(16645..17385)
                     /locus_tag="WGO65_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(17455..17772)
                     /locus_tag="WGO65_000407"
     CDS             complement(17455..17772)
                     /locus_tag="WGO65_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326753.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(17769..18380)
                     /locus_tag="WGO65_000408"
                     /pseudo
     CDS             complement(17769..18380)
                     /locus_tag="WGO65_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(18464..18766)
                     /locus_tag="WGO65_000409"
     CDS             complement(18464..18766)
                     /locus_tag="WGO65_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326754.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(18945..19151)
                     /locus_tag="WGO65_000410"
     CDS             complement(18945..19151)
                     /locus_tag="WGO65_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326755.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(19117..19866)
                     /gene="mafB"
                     /locus_tag="WGO65_000411"
                     /pseudo
     CDS             complement(19117..19866)
                     /gene="mafB"
                     /locus_tag="WGO65_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(20292..20636)
                     /locus_tag="WGO65_000412"
     CDS             complement(20292..20636)
                     /locus_tag="WGO65_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326756.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(20674..21018)
                     /locus_tag="WGO65_000413"
     CDS             complement(20674..21018)
                     /locus_tag="WGO65_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326757.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(21021..21209)
                     /locus_tag="WGO65_000414"
     CDS             complement(21021..21209)
                     /locus_tag="WGO65_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ0326758.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(21279..21583)
                     /locus_tag="WGO65_000415"
                     /pseudo
     CDS             complement(21279..21583)
                     /locus_tag="WGO65_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT         5125 a         5052 c         5419 g         5987 t
ORIGIN      
        1 tcgggcattc cttaaattac ctgtgtatcg ctgtaaatct tagagatggc ggaatatagc
       61 ggattaacaa aaaccggtac ggcgttgcct cgccttggct caaagagaac gattctctaa
      121 ggtgctcaag caccgagtga atcggttccg tactatttgt actgtctgcg gcttcgtcgc
      181 cttatcctga tttttgttaa ttcactatat cgattctgtt cccttgcagg cggacggcgg
      241 cggcggacgg tggaaaagca ggttttcaaa tgtccggttt ggcggcgcgc cgtctgaaaa
      301 ccgcttattt tcctttgccg tttccggcgg agtctttttt gtccgaaccg gagaagcggt
      361 cgaaacgtat ggtgtatgtc agctcgccgc ccgacgaacg gctgccgata cgggcaaccg
      421 cctgtatggc gcgggtcagc cggtaaatca gtttgacgga ctgttccgcg ctggagatgc
      481 tgtattcgta gccgatgtag agtttgccgg tcagttgttt gccgacggtc agcacctgtt
      541 cggcggggtt gagttcgccg gtttgcgcgt tgcggctgcg cttgctggta aagcccaaat
      601 catccaccag cccgatgcgg tcgttgattt gcccggcaag cagcgcgcct gcggctgcgg
      661 acagggcggc attgtcgccg ctgctgccgc tgccggcgcg gttgaggatg agccaagaga
      721 gcttgtcttt ttcactcatc ggttcgtttg ccgtcagcgt aatgcgcggg ctgttgaggc
      781 tgcccaatat ttccacgccc gcaccgacgg gggaaaggcg gcgttcggcg cggatgttga
      841 ggttggggtc gttgagcggg ccgacaaagg agaccgtgcc tttggtaatg tccaaatcct
      901 gcccgtatgc cttataacgc cctttgatga cgcggaccgt gcccacgccc cgcacgcttc
      961 cgcccgattg ggcggtcagg gtcagtttgc cgcctatggt aacgtccgcg ccgtagccgg
     1021 cgaagcggat gccgtcattg aggtctaaag tcaggttcat attgacgggg agcggtgccg
     1081 ccgcctcttt tttgacttcg cctaatacga cgacatcgtc gccgacggac ggcatcgagg
     1141 atttttgcga accgaacagc ccctgatccg ttttaatcat cccggtaacg gatatgcctt
     1201 tttgcggcga atagcgcagg cgggtgttgc cggaaaccgt caggcggcgg ttggggcggg
     1261 acaggatgcg gtatttgtcg aacaccgcgc cgatatcgac atcgggtccg ctgttttcca
     1321 taccgaccgt accggagagt tccgccgtcc cttcgtgacg gaatttcagg ctgtcgatta
     1381 cccatttcct gcctgcgata tgcgaacgca gcgaaccgtt gtccaagatg atgccttggg
     1441 tttggttgcg gtaatagagc ttgtcgccgt tgatgctgcc gcccaagtgc gggtcggcga
     1501 tgctgccgcc gagggttacg gcggcattca ggctgccttt gacggtttgt ccgacgggca
     1561 ggaagttgcg gaatacttcg gcatcggcaa cggtcaggtt gagtttcccg cccaggggcg
     1621 aggtactgaa agaattactt tgcccgacgg tgatgttgcc gttgattttc ccgtagttgc
     1681 tgctgccgtt gacggcggca ttgacggacg gagagccgac ccgtccgccg atttgcgcgg
     1741 cggcattcag gctgccggta atgttttgcg cggcggcggg cagaaagggc ttcaatgcgc
     1801 ccaagtcggg aagggaggcg gtaatcctgc cgccgagcgg tgcatttgcc atattgccgc
     1861 cgaaggcgtt gccgatgtcc aaatcggcgt taatccgccc gaaacgcgcg ccgccgtcaa
     1921 gcaggattcc gatgcggtcg ttttgaaagc gcgttttcag ggaaaatgcg ttcaaaccca
     1981 aagcctgccc gccgggcaat acggcatcgc cgctttgtcg gctgatattg aggtagccgc
     2041 gcgcgttgcg cccgtaggcg acatcccagt cgccgtttaa aaccagattg tgttcgaagg
     2101 acggtttgaa gaaattgtgc aactcggcga tatgcagacc gtgtgcgccg ccttttgccg
     2161 atatgccggt ttttttatcc caagaaaagt gttgcaggtt gaggctgccg cccattgcct
     2221 gccaatttgc cgcacttgcc gccacgcgtt ccgcaccggc ttcgagcgtc atacggtttt
     2281 gcagcttgag gttgaatgcg ccgccgatgt cgaggatgcc gatgctgcct ttccatcggg
     2341 taagttccct gttgatgccg cctgaagcgt ccaaatcgaa tttgaacggt ttgccatcca
     2401 gcgtcatggc ggcgtgtgtg cggatgcggt gctgcacgcc cgtgccgtcc agcatcaggt
     2461 cggcggtatc gacaaccgcc gctccgcccg acagcgaaag gcggctgcct ttgatgtcgg
     2521 cgcgtatcgg gcggcttgtg tcgggcgaac ctttgagcgt gaaatcgagc gaacggatgt
     2581 ctgccgcctt gccgatgtgc aggttgcgcg ccgcgccgga aaggtcggtt tcaaaggttc
     2641 ggatgccgcc gtccaaatcg ccggaaaggt gtccgcgtac atttaaagac cccgcgagtc
     2701 cgaaaccgaa acgggataaa tcgggtgcgg tgatattgag gttaagccgg tcgccttttt
     2761 tgccgaagcc gccgtctgtt ttgatgatgt tccgccccag ccgcaaatcg acggcggcac
     2821 gcggaaggtg gcgggactcg taaacaatgt cggcactgcc ggcaatcggt acgccgttga
     2881 acgtgccggg taaaaaccgc attttgcctg tgaatttctc ttttgccagt tcgccggcaa
     2941 ggtttattga gccgttgata ttgcctgccg gaagttgcgg atcgatgcgc gaagggtcga
     3001 atgcgcggga acggatgtcc agcttgagca ggcggtcttt aaacagctcg agatagcctt
     3061 gcgcggtcag gctgccttgc ccggcggcga tgttgacggt gtcgagcacc agtttccgct
     3121 gtccgtttgc ggggtcgctt gcaatggcga ggctgccgtc cgtgcgcgcc gtgccgatgc
     3181 cgagttgcca agagattttg ggcgaggcgg tcgtgccacc gatgccgatg ctgccgtcca
     3241 acctgccttt gaacgcggtt tgcagtacgt cttccgcgcc gacggagttg atgcctatat
     3301 ttaaatcgag gatgtctttt ccggtgtcga ttttgcccga cagcctgatg ccgccccgtc
     3361 cgagcagggc ggcggacgta ttgccgatat gcaccgtgcc gtcctgccgg atgacaaagc
     3421 tgcctaaaac ctgacggacg gggatgccgt tgcggtcggc aaaaccggct ttggtgtttt
     3481 ccaaatcgag cgagccttcc agcgcgatgc cgtctgaaaa cgacgggatg gcggtcaggt
     3541 cgaaattcag cccggcatcg ggcagggaag gtacgaaggc ggccggattg atgttgaacc
     3601 ctttgaccag tacttcttcc aatgttttat ccaatgattc ggcaaacggg tggatgacgg
     3661 attttcccga gaggcggata ttgccgccgt cgatcgccag ttcggcgcgc acatccttca
     3721 ggctgccgct cagccgagcc gtactgtgta tggttttgcc ttcgagtccg cctttggtgt
     3781 aaatggcggt atcgagggca aacggttttt tcaagccgac cgaggccgcc cccgacgaac
     3841 tgctccacgg cgtgtcggcg gccttcaggt caaggcggtg tcctttgcgg tcgtaacggt
     3901 atgaagcatc cagccgttcg agatagacgg tttgtttgtc aaaggctttg cccatgctga
     3961 ttttgcccgt ctcgaagcgg tcgagataga cggcggcagg caggtctatg ctgtcgggaa
     4021 ggctgagcgg cgggcgttct tctttaggcg gagtcggttt ggtaacgatg gcgatgtcgc
     4081 cggcggaaat ttcggtaatg tgcaggctgc ggtgcatcag ttcggacggt ttccacgcga
     4141 agcggaagcg gctgatttta aggtctgccc cctcggtttc tatcgaccaa ttgtcgccgt
     4201 cgaagccgtc gagcagcgtg cctttgaggt tttgggagga aatgtttacg ccgaaccaag
     4261 acgggatttg gtacagcccg aagcgcaaac ccgcttccgt gccggcgagc cagccgagga
     4321 aacatactgc caaaatcaga acagacagca gtgccgccga cagcttcagc agcgggcggc
     4381 gtttttttgc cggcggggta gggcggtgtt cagacggcat tttgcgcgtg ccgttttcgg
     4441 tcggatcggt atctgtcggt gcggtatcgg tcataatgtc tgtttcaaaa tggtttgctg
     4501 caatgccgtc tgaagcggcc gtatcggttt agaagcgcgt tcccaagctg atgtgccagc
     4561 ggattttctt gtcgctgtgc ccgtaggcga tgtcgaagga aaacggcgcg agcgggctga
     4621 accagcgcac gcccagtccc gaaccgtgtt tcagcttcat acgtttgaaa ttggcggcgg
     4681 cgtcgcccat atcgtggaac accgcgccgg aaagggtgcg cgtaaacggc agttggtatt
     4741 ccaggctgcc caccaagagg gcgcgttcgg gcaggaccga tccgttcggg ccggcaagcc
     4801 cgatgctgtc gagttcgtaa ccgcgcacgg aagacgcgcc gccgctgcgg aacatcagcc
     4861 ctgaaggaac gttggcattg tcgcgggcaa cggtgtaacc cgcttgtccg cgtatgatga
     4921 acgtgccgag ttttttgttt tcgggcgtga agaaataacc tgcgcgggca gaggtgcgga
     4981 tcagcgcggt ggaggacagg aatgcgccca aagtcgtacc gattttgccg tcgaggtaat
     5041 ggccgttttc gggatgcagc acgttgttga gcagctggcg tttccaagag gcggtcagca
     5101 tcgtggcgtg gctgttgccc aaatcgatat ccgagccggg gattttccgg ccttctgcga
     5161 gaaactccgc ccccagcctg gcatcgatgc ccgcgcggtc gcgcacatac cagatgccgc
     5221 cggagaaggc gcgtttttcg aggttttggg tggtcgaacg gttgtaggaa acgttgcttg
     5281 tccagtagtt gccccgatag ttgcgcggct ggctgatgcc ggcggcaagc gtggtttcgt
     5341 atttgtccat atcccagacg accgaaccga tatagccttt gttgaagagg ttgtaatagt
     5401 cgtaggcgat tttgccgccc aaaccgtatt ccgaatcgag gcggatgccg gtttcgagct
     5461 tgtggcgttt gacctcggtt acgctgactt tgacggggac gcggtcgcct tggaggcggt
     5521 cgaagtcggc ttgtacggac gcgccggaat aatgcccgtt ttgttcgagc gcctgttgga
     5581 agtcgagcag caggtcgagg tcgtagggcg tgcccggttg gaagcgcgcc aggccggaga
     5641 cgatttgttc ggggtaacgc tgcgtgccgg taatttcaaa gtcgccgaag gcgatggggc
     5701 ggccgctgtc cacgacgacg ttcaaatcgg cggtggcggt atcggggttg acggccgccc
     5761 gggtgttgcc gagcttggca agcgggtagg ctttgcgcgt taccgcgccg aggacggaag
     5821 ttttgctgtt ttcccaactg tcctgatcga aatcactgcc taccggctgc tgccagtttt
     5881 ccagcgcgtt gcggtagtat tcggcgaggt tgccgtctga aaggatgtcg ccgaggatgg
     5941 cgacgccgac gttggcgatt ttggtgcgcg ggcccggtgt gatgtgtacc gtataagctc
     6001 cgtctttttc cgtcaggctg actttgctgc tgaaatagcc tttgctgcgg agcatcgttt
     6061 taacgttgtc cgatgcttct tcggcgagga agcccgtctg ttctttgtcc aatacttctt
     6121 cctgctgctg cgtgatgagc ggcaggtgtt cttcgaccat atctttgatt tcactgtcct
     6181 gcgtgtcgat gcggacgggg aatttgggtt ttaatttgac tgattcggtg tcggggcttt
     6241 tgtttttgaa caatgcgaaa cccgccgcct tgttttcgga aaggtcggcg gcaggcgcgt
     6301 atgcgtgcgg aaagaaaaat aaagccggca ggagcagggc ggtcggttta atcatcatgg
     6361 tacgggtatc gtgcatcggt gtggcggatt tcgattctaa cactgttcgg acggggcggg
     6421 acgcttcaga cggcatactg ccgccggtat ttcggaggtt gcggcccgct ggaaatgtat
     6481 ctgcttgttt taaggtattt tgtttctaat ccgcatatgg aaaacaggct gcgtttcgga
     6541 atagtgtcgg aaaataaacg ggattaaata aatttatttt cgttcaataa attaaaccgt
     6601 cgcgccgaat gataaagcaa agcctgtccg gcactgcata gggcaaatca acgcaacctt
     6661 catagtggat taacaaaaac cagtacggcg ttggctcgcc ttagctcaaa gagaacgatt
     6721 ctttaaggtg ctgaagcacc aagtgaatcg gttccgtact atttgtactg tctgcggctt
     6781 cgtcgccttg tcatgatttt tgttaatcca ctataaaaca cagccgaaaa ataaaaaaaa
     6841 cacagagaat gagttttctt ttaaaataca acatttttta acatatcaca acataaggaa
     6901 gaattatggc gttcggcaaa tccttatttc atgcgatcgg ccgcgtcagc ctggtcaggc
     6961 agattgccgc cggtttggcg ttgggcatcg taatcggttc ggtttccccg caactgggct
     7021 tggcggcagg cttgttcggc agcctgtttg tcggcgcgct caaagcggtc gcgccggttt
     7081 tggtatttat tttggtggcg gccacaatcg cgcagcacca aaaaggcaac aaggcgcata
     7141 tcaggccgat tatcatcctt tacctcatcg gtacgttttc cgcagccctg accgccgtca
     7201 tcgccggtat ggttttcccg acgcacattg ttttggcggg cgcgggcgat gtgtccgccg
     7261 cgccgccttc cggcattgtg gaagtattga aatcgctgct gatgaacctg gtcgccaacc
     7321 cgattaacgc gattgccaat gccaactata tcggcatttt ggcttgggct ttggttttgg
     7381 gcgcggcgtt gcggaatcac ggttcggacg ttacgcggca ggtcgttgcc gatttggcgg
     7441 aagcggtttc caccgtcgtg aaatggatta tccgttttgc ccctttgggc attttcgggc
     7501 tggtgtcgtc cacaatcgcg gagacgggtt tcggcgcgct ggcgggttat gcgaagctgc
     7561 ttgcggtact gttgggctgt atggcgttta tcgcgctggt ggtcaatccc gccatcgtgt
     7621 ggtggaaaat ccgccgcaac ccttatccgc tggtgtttac ctgcctgcgc gaaagcggcg
     7681 tgtatgcctt ctttacccgt tcttccgccg ccaatatccc cgtgaatatg gctttggcga
     7741 aaaaactggg gctgcacgaa gacacttatt cgatttccat cccgttgggg gcgaccgtca
     7801 atatgggcgg cgcggcgatt acgattaccg ttttggctat ggcggcggcg cacactcaag
     7861 gcatacaggt tgattttgcc accgcgctgc tgttgagcct ggtggcgacg gtcagcgcgt
     7921 gcggcgcgtc cggtgtggca ggcggttcgc tgctgctgat tccgctggcg tgcagcctgt
     7981 tcggcatcag caacgatgtc gctatgcagg tggtcgccgt cggctttatc atcggcgtga
     8041 ttcaggattc ggcggaaacg gcgttgaatt cttcaaccga tgttttgttt accgctgccg
     8101 ccgatttggg tcgacagaga aaccgggcgg aataaaccaa gctatgccgt ctgaagcccg
     8161 tttggcgttc agacggcata tttttacaat tttatgggca tatggcggaa acaataccgg
     8221 ctgccttgaa tatgaagggg atgatgatgt ttaaacgcag tgtgattgca atggcttgta
     8281 ttgttgccct ttcagcctgt gggggcggcg gtggcggatc gcccgatgtt aagtcggcgg
     8341 acacgccgtc aaaaccggcc gctcctgttg ttgctgaaaa agagacagag gtaaaagaag
     8401 atgcgccaca ggcaggttct caaggacagg gcgcgccatc cacacaaggc agccaagata
     8461 tggcggcagt ttcggcagaa aatacaggca atggcggtgc ggcaacaacg gacaaaccca
     8521 aaaatgaaga cgagggaccg caaaatgata tgccgcaaaa tgccggcaat acggctgccc
     8581 aaggtacaaa tcaagccgaa aacaatcaag tcggcggctc tcaaaatcct gccccttcaa
     8641 gcaatcctaa tgccacgaat ggtggcaatt ttggaagggt tgatttggct aacggtgttt
     8701 tgattgacgg accgtcgcaa aatataacgt tgacccattg taaaagcgat tcttgtaatg
     8761 gtgataatct attaagcgaa gaagcaccgt caaaatcaga atttgaacaa ttaagtgatg
     8821 aagacaaaat taagaaatat aaaaaagatg gagaaaagtt taccggtttg gttgctgata
     8881 ggttacagat gaaaggaacc aatcaatata ttatttttta caaacctaaa accacttcat
     8941 ctgcgcgatt caggcgttct gcacggtcta ggaggtcgct tcccgccgag atgccgctga
     9001 ttcccgtcaa tcaggcggat acgctgattg tcgatgggga agcggtcagc ctgacggggc
     9061 attccggcaa tattttcgcg cccgaaggga attaccggta tctgacttac ggggcggaaa
     9121 aattgtccgg cggatcgtat gccctcagtg tgcaaggcaa accggcaaaa ggcgaaatgc
     9181 ttgctggcac ggcagtgtac aacggcgaag tgctgcattt ccatacggaa aacggccgtt
     9241 cgtacccgac cagaggcagg tttgccgcaa aagtcgattt tggcagcaaa tctgtggacg
     9301 gcattatcga cagcggcgat gatttgcata tgggtacgca aaaattcaaa gccgccatcg
     9361 atggaaacgg ctttaagggg aattggacgg aaaatggcgg cggggatgtt tccggaaggt
     9421 tttacggtcc ggccggcgag gaagtggcgg ggaaatacag ctatcgcccg acagatgcgg
     9481 aaaagggcgg attcggcgtg tttgccggca aaaaagagca ggattgatgt tgatgccgtc
     9541 tgaagcgagg gcgttcagac ggcattgtga tgtttgggtc gcgggtccgg ttcaagtccg
     9601 catcagttga taaacccgtc gtctttcatt ttcttttcgg aaaaatattg accgcatacc
     9661 agtaaggcgg cgaggatgat gacaaacagc cccgccatag tcagtgtgat tttgaagaac
     9721 aattccgctt cgagaaaaga aaaccaaagt tgcacaatca gcagcagcac ccagccggca
     9781 aacagtccga gacaggcata aacgccgagc ttaaacacgg tcccgctcca aaatgacgac
     9841 gtaggcctcg cgtttgaaaa acaacagcat acggcgcaaa tctttttcca tcgttacaac
     9901 ccgccagcct tcgggggttt gtttgttgag gaacgcgctg aaattgacgg ggttgacctt
     9961 tgccgcgccg aaaaacaggc tggacaacag gctttcctga taaatgacga ctttgtattc
    10021 tttcatagga ttccttttaa cataagggat catcttttca gacgacccct taagttgcta
    10081 atgatgcaaa acagcaaacc ttatttctta ttgcccaact gcggcaatac cgaaccttcg
    10141 cccaatgcca acaaaccgct ttgcgagtaa atgcccagtt tggcgcgggt gtcggtaatg
    10201 tcgaggttgc gcatagtcag ttgtccgata cggtcgagcg gtgtgaacgc tgcgtcttcg
    10261 actttttcca tactcaggcg ttcgggttgg taggtcaggt tgggcgattc ggtgttcaga
    10321 atcgagtagt cgttgccgcg acgcaattcg agggttactt cgccggtaat ggctttggca
    10381 acccagcgtt gggcggtttc gcgcaacatc agggcttggc tgtcgaacca gcggccttgg
    10441 tagagcaaac gtccgaggcg caggccgttg atgcggtatt gttcgatggt gtcttcgttg
    10501 tggataccgg tcaccaaacg ctcgtaggcg atgtggaaca acgccattcc cggggcttcg
    10561 tagatgccgc gcgatttggc ttcgatgatg cggttttcga tttggtcgct catacccaga
    10621 ccgtggcggc cgccgatgcg gttggcttcg aggaagagtt cgacgggatc agcgtattct
    10681 ttgccgttca gtgcaaccgg cacgccttct tcaaagcgca cgctgacttc ttcgggtttg
    10741 atttcgacgt tttcgtccca gaaggcaacg cccataatag gtttgacgat tttgatgccc
    10801 gagttcaaaa attccaaatc tttggcttcg tgggtcgcgc cgagcatatt ggaatcggtg
    10861 gaataggctt tttccaccga cattttgtag ttgaaaccgt tggcaatcaa aaattcgctc
    10921 atttcatgac ggccgccgag ttcgtcgata aattgctggt cgagccaggg tttgtagatt
    10981 ttcagcgcgg gattggtgag caaaccgtag cggtagaaac gctcgatgtc gttgcctttg
    11041 taggtgctgc cgtcgcccca aatattcaca tcgtcttctt tcatggcgga aacaagcata
    11101 gtgccggtta cggcgcgacc cagaggcgtg gtgttgaagt aggcgatgcc gccggtggaa
    11161 acgtggaacg cgccgcattg gatggcggcg atgccttcgt gtgccagctg tgtgcggcag
    11221 tcaatcaggc gggcgttttc cgcaccgtat tccattgctt ttttgggaat ggcattgtag
    11281 tcgtcttcgt cgggctggcc gaggttggca gtgtaggcat aaggtagcgc gcctttgagt
    11341 ttcatccaca acagcgcggc agaggtatca agaccgccgg agaaggcgat gccgactttc
    11401 tgaccgacgg ggaggttttg taaaatagta tggttttggc tcataagggt atccttcgga
    11461 cttggaaaaa ggagttcgtc gtgtttcctt ttcgattgaa accccgccac ttggacatct
    11521 gtccttcggg gcggtagaat cagactttat ttgggagggg cgtaatccct tccaaatcag
    11581 gacggcacat agggcggtgc tttatgtgtc gtcccgtgtg ttgaaacatt atcttataaa
    11641 tataacccat tgtcagccgt taaggctgtc tgaaaagaag ctttgcccgt ttcgctgaaa
    11701 atcttttcag acggtcttta cgccatcctc aacctgactc cctcccctgc gggggagggt
    11761 tggggagagg gtgcagacag cagcccttaa gcaaacgctg cctgaaaaac gcaagctcga
    11821 ccctctccct aaccctctcc catgggagag ggaacaaatc acagcaccat cgacattttc
    11881 agacggcatc ggtaaagaaa cccatctatg aacgcgttca acctcaccat catcggcgac
    11941 gaaatcttac acggcagccg ccaagacaag catttcgcct ttttcaaatc cctgctcgaa
    12001 tccaaagggc tgaagctcaa tcaggtgcaa tatctgcccg acgaacccga tttgctggtc
    12061 aggcaactgc gccgcagctt ttcagacggc ataccgacgt ttgttaccgg cggcatcggc
    12121 gccacgcccg acgaccacac gcgccaagcc gcagcggctg ctttggattt gcctgtcgtc
    12181 cgccatcctg aagccgccaa gtttatcgaa ggcgtaaccc ggaaacgcgg tgagccgctc
    12241 gattcgcccg aacacgccca acgcctgaag atggcggatt ttccccaagg cgcggaattg
    12301 gtgcctaatc cgtttaataa tatcgccgga ttttccattc gcgagcatta tttcttccct
    12361 ggttttcctg tgatggcgca cccgatggcg gaatgggttt tggatactta ttacgtcggc
    12421 cgcttcaacc aaaccaaacg cggcagccgc agcgtgtatg tgtttgagca gcccgaatcg
    12481 cgcattacgc cgcttatgga gcatatcgag caaacttatc ccggtgtgcg ttcgtacagc
    12541 ctgcccagcg tcggttggac gcattcagac ggcacgcagg tcaaaccgca cattgagttc
    12601 ggcatcaaag ccgaaggaga agccgtcaac cttttggacg cggcgtgggc ggaagtgttg
    12661 cacagcttgg acggattggg agcagagctg aaaaatcggg taaactgaaa accgtttgcc
    12721 caaatcggac gatatttaag gaaaaccatg caataccgaa tcagaagaga aaacgaagca
    12781 cccgaagcaa aaaatacggg ggaaacctta tgggagcgcg acatcatgcg cgaagtgctg
    12841 ctgtccgcct atcaggacag gcgcagggag cggatgtgga aaaacatctg gcgtgccgtc
    12901 agcaccctga ttctggttgc cctgattgca ggcattttcc gaaaagacga agccgcattg
    12961 cagttggcgg gcaacacgcc gcataccgca gtcgtcaacc tgtacggcga aatcggcaac
    13021 ggtgtagaag accaggtcaa aaaactcaaa gacggtatgg aggccgccta caaaaacccg
    13081 caggcaaaag ccatcgtcat ccgcgccaac agccccggcg gttcgcccgt cgtctccaac
    13141 accgcttttg aagaaatacg ccgtctgaag gcgcagcacc ccggcattcc cgtttatctc
    13201 gtggcggaag atatgtgcgc gtccggctgc tactacatcg cggcggcggc ggacaaaatc
    13261 tatgccgacc cgtccagcat cgtcggcagc atcggcgtta tcggcagcag tttcgacgcg
    13321 accggcctga tggaaaaaat cggcgtgaaa cgcagggtta aaattgcagg cagcaacaaa
    13381 ggcatgggcg atccgttttc gcccgaaacg cccgaacagt cgaaaatctg ggaagaaatg
    13441 ctgaccggca tacacggcga attcatcaaa gccgtcaaaa ccggacgggg cggccgcctc
    13501 aaattccgac aatatcccga cgtgttcagc ggtcgcgtgt acacaggcgc ggatgcgctg
    13561 aaagtcgggc tggtggacgg actcggcaat atttacagcg tggcgcgaga cgtggtcaaa
    13621 gcccccgatg tggtggatta cactccgaag gacgatttcg gcagaatctt gggcagacgc
    13681 ttcggcgcag aactcaaagc aagcgtgcgc gaagccctgc aggccgtccg gtagcgttgt
    13741 ttacagtatt tctaagggaa taccaaaata aaaatataca caaaaacact cacctcccca
    13801 gtatgctgcc actaagtcag ccgcgtcact atttaccgtg cacttaaagc cgtaaaaggc
    13861 aggttgtccc cgggaggttt tgtgcatcct gataccgatc ggttgttgtt gctcaaagga
    13921 cagaaggccg ctgataaacg agattacctg tttgtcgcta ttgacgattt ttatactctg
    13981 ccattttgct agacaaaacc gcagacagtg ctgccaagtt tctgaccgaa catctggccg
    14041 acccctgctt gtacctgatt gagtacgttt actctgacaa tgataggtaa tataaagagc
    14101 cgtccaacat gctttcggtg cagcttgtta taataatggg attggttgga ggcttgcccg
    14161 atttgcttgt ccgcagacca acggtaaggc ggagcgggtt atccgtacct tgatggagat
    14221 gtggcatgag gaacagtcgt ttgacagtcc ggaacatcgg caaaaggagt tgtgctgctt
    14281 tattaattta ggggctaccc tagatcacta gggaaattca aattaagtta gaattatccc
    14341 tatgagagaa agtcgtctaa gccggtataa acaaaataaa ctcattttgt tatcgtggct
    14401 atgatgtatt ggatgcgggc gaatttagcc atttccgtat caatcacagc acacattttg
    14461 ccgaacgaca aaaccatatt aatggaactg agaacttttg gaatcaagca aaacgtcatt
    14521 tacgcaagtt taacggcatt cccaaagcgc attttgagct gtatttaaag gagcgcgaat
    14581 ggcgttttaa caacagtgaa ataaaagttc ttgttccatt ttaaaacaat tagtaaaatc
    14641 gagtttatcc tagttgtcca gggcagcctc atctctttat agatagctgc ttaccaagta
    14701 tccttaattg ctaattcaag ccaatcttca aaactattaa aaaattcatg gttttctata
    14761 ttatctaaat taatgacaat aaccctagaa tttcctgtta catcattacc atcaaaataa
    14821 gcttcccatt ctccattact agcgaatggg ataagattta aatcaggcag gatagaattt
    14881 ctaaatatat aagctacttc tgccccttct gttccataat tctcaaacca ccagtgaaaa
    14941 ttatgtggct gaatataagg tattaaagat gtatcgttag agatacgaac atacttttca
    15001 ggataacaaa acctttcagg aagcatatta ctaggatata aattaaattc attttaaata
    15061 atcatttcgt taccttagga tcataaatac taccattagg aaactgggta gaccaatttg
    15121 gtggtgctag ttcattttca atttgttttt taattctaaa ttgaatagct tctccccatg
    15181 tttgcggcat ctaatgctgc ttgctctgca tcaatattac taaaaattgg attagtctca
    15241 aaaatgaaaa attactgttt tttcattttt tatagcatat tcaagtatat gaataatatt
    15301 tttcactaag ggctctgtag aaaaatctga attttgtaat ttttctttta cagccaaaat
    15361 actttcctta tctattagcc aatccccctc acttggtcca aataaccaat tatcggtaat
    15421 ttcagaaaca agtctccata atccacgatt ttgatattca ataatttgac tttccgtaat
    15481 ttctacaact gtttcagatt gaatgttttt ataagcccaa tcaatcattt cagtatattc
    15541 actaattgaa acattgtctt ttaaacagaa aattaacagg ttgtgttcat tcatttgtct
    15601 aagttcttaa tatgagtttg ttgttgtaaa taatcttttg cttgcttacc ttgtaaatta
    15661 aaggattaac cgcaccttgg atgatgccgc gaatatcatc catacggtta ccccaccaaa
    15721 ttttattggt gtatcgttaa tcatttattt caaaaaagtt atttattatc atattatcag
    15781 ggagcaggtc gttaaaaaaa accccctcaa attccattaa attagtattt atccagcaca
    15841 taaacgaacc aaagcgttct ttccctgagt gtaaatattt aaaattaaac tcaaaataag
    15901 tttttttgct tagaaaattt aatggttttt ctatagtgaa aaaaaaagcc cttactcgtt
    15961 ttagttcgag atactatttt aaataaattt ttattttcaa taaaataaga atattcattt
    16021 actaagttgt tatgataaaa aagtaatttt attaaaatgt tttcataatt ttctaggttc
    16081 atttctagct caatttaaaa tgatttcatt gttgccgtac caatgcgaaa tttaccatca
    16141 acaaaatttc ccttcactga tacaggtacg ccatcaactt gggtagtttt attttcaggc
    16201 tgcctgaaag aaagataggc aacttattac tcatcttacg ctagccctca ataattgaat
    16261 acgttgcact agaaccaatt atttctatga atgtttgctc aaacataaaa atatagtatt
    16321 taaagaattc tctattcaaa ggaaaataac tttctttcaa tatccattcc gcataacact
    16381 catcaagaag ttcaaaaaag attgaccacc attttcatga agcaaagctc tatctgcatc
    16441 ttccgaaatt aaatccataa aagtatctat aaaatacgat tcatcaacaa ccctaaaatg
    16501 aataacatct tcaataaaaa tttttatggt tttatccgta tttctataac caactgctat
    16561 aacttctact ttatcagagt ttattgctgc attatcaaaa tagaaatagc cagttgggaa
    16621 atctaagctt tttaatttca taatttatcg tgtatttcca tatctgattt ttatttttcg
    16681 gttattacta attgttattt caagtgttgg ttctccacca ctactatcat tacgagcatt
    16741 aaccgcacgc ctatcaggta aattgcccgt tatcgttcca ttaggacgtg tttgtacatt
    16801 tcgaacattc agactattaa aatcatttag tgcgtcattg taatccccca ctttatcata
    16861 ctgaacagcg ggaccttttg ttttactcct ttgagtagta ttgctcaaaa tgtctttgat
    16921 ttgtaaatca ctgcgcacct taccggtttc tatgcgagct gccacactac tcgggctata
    16981 ggggtcgcta tcaatcgcag gattaccacc gctggttttt tgtccgctag cctgtttccc
    17041 aacctgctgt actgtatcgc caatttcatc taccgtacct aacgccttag tctgcatatg
    17101 gcgaacagca ggttttttat agccggtatt tttaacccaa tcccatttag tcgggttaag
    17161 ttctactttt ttacctctcc aaaccgtacc tgcggcctct gctacggaaa gggcagtttg
    17221 ggctgttgca gttatattcg gatgggcatc agcccattgt ctggcagagt tgatactgtc
    17281 ttttaccgca aaagcactgt cttgtaatgc ggttgcggcc gcaagttgtg cttcgggact
    17341 taaatttcct aaattattaa taccttgtag agtctgctgc gcagagatgt agcaactgta
    17401 tttttacccc gtcgggtaaa aatacagttg ctacggctgc tgggaaagca gttactattt
    17461 tcccagtggc tcaaaattaa ttgtttcagc agatattttt ataataatcc ctacatcaat
    17521 aataatttca ctataaaatt ggtatttata tagctctatt tcgggtataa acaagggaaa
    17581 caagctatct tccttatcct cataatctac gaaattattt gtatctaatt taaaattcaa
    17641 aatattactg catttgatat atccgaaata attaccttca attgtatctg taaaagataa
    17701 tgtaagggta tttccattat cggaaaagat tatttcataa aggcttacat tgtgaaaaat
    17761 attttttttc atttgacaaa atatccagaa tgatgacgat tatttggcaa tggattgcct
    17821 tcataatctt gtcgttgcgg atattttccc tgtacaccat cggttcgcgg cactctttgg
    17881 gttcttataa cctctccatt tggacgaata tctattttca cgccatcagg acctttccat
    17941 gtttcatatt gagcctcatc tgaaccagta cgtctaaaac cttgttcttg caatgcttga
    18001 cgtaattcat ctcgcgtagt tggtctgata gatttaacaa ctggcttgtt tcctcctgcc
    18061 atctcaccgt ccaacgtctg cataggtcgg gcagcaggtt ttttatagcc ggtattttta
    18121 acccaatccc atttggtcgg gttaaggttt acttttttac cgccccaaac cgtacctgcg
    18181 gcctctgcta tggcaagagc agtttgggct gttgcagtta tattcggatg ggcatcagcc
    18241 cattgtctgg cggaattgat gctgtctttt accgcaaaag cactgtcctg taataggctc
    18301 gcggcagcaa gttgtgcttc tggacttaat tttcctaaat cattaatacc ttgtagagtc
    18361 tgctgagcgg ctgtatctga gccggtcagt gtaggttgga ttaaaaccca gcattttcaa
    18421 catcttgatg ttttactata aatctattct tgtgcttcct ttgttagata aatttcaaca
    18481 caaaagatac gtatgcattc tctaatttta tttgagataa ataatgcatt gctttgtcga
    18541 tactgttaaa taaattactt ccctcgtaat accaattatc atatgtttga taaaaataat
    18601 tatctttctt gatataaata tctcccccca atacaagtat tctctcattg ataaataaat
    18661 ttaatatatc tattgcaata gggacgggta atgcggcttc tctaattcca attcctatca
    18721 aagattcccc ttgtctcgaa taaatttcaa aaatttcatt taacatgtta atctctatct
    18781 actatttttc aatcggtata aaccctattc acattttatt tttcctaaaa ccaaatcttc
    18841 gactaactgg gttgcataaa taatatcatt tatgatttta acaggtaaat tatttgatag
    18901 gtaaccatgg gattccgtca tcattgattt ctctaggtgc ttatttatct gattggtaag
    18961 ccttttgcgt aagcttcttt cgcctccgca agtgtcattt cattaggtcc accatctata
    19021 ttagtttctc ctgtattttg ccactgacaa acgtcacaga tatcatagtc cataacttca
    19081 gttccgcaaa caggacaatg tagccatatt tctccgtcaa tttcccatgt cttttttgaa
    19141 ttctccatca taatacttct ttcctaagtt tggtttaaac attgttttga ttttgaatgt
    19201 ttttggattg gccaaaacat agatattgtt attgatatca tagcgcactc gtctatgttc
    19261 cgtctgataa cctaatatct tgtccgatgt ctgttttgat aacaaatcag acgccatttt
    19321 ttggtagtct tcaatggtta tatcgccaaa ttcttttcca tgagctttga aatgtccatt
    19381 taaagatttc tcagtaggaa acttggactt tgtccatctg atgcggtctt gaattgttcc
    19441 tgtcggagaa atagatgtat caggtttaac atatgaggca ggtaagccga tattgttatc
    19501 agagtgtgta gggtgttgct gcgtacttgg ttttggtggc ttattcccac ctgccatctc
    19561 cccatctaaa gtctgcatag ggcgggcagt aggtgtttta tagccggtat ttttaaccca
    19621 atcccatttg gtcgggttaa gttctacttt tttaccgccc caaaccgtac ctgcggcctc
    19681 tgctatggca agggcggttt gggcagtagc tgtaatgttc ggatgggcat cagcccattg
    19741 tctggcggaa ttgataccgt cttttaccgc aaaagtactg tcctataatg cgcttgcggc
    19801 agcaagttgt gctttcggac ttaaatttcc taaatcatta ataccttgta aagtttgctg
    19861 cgcagagatg taggtcggat atttgtatcc gacaaaaata tttgacgcat ctatcgtttc
    19921 cgaaatgccg ctgttggaaa atgtcggatt cgagaatccg acctacggct atttcgttta
    19981 agtcataaat agccattcat gttccttctg aatttgcttt tgctatcctt agaaccaatc
    20041 atataaacat tatcatcatt aattaatgtt ctaagtttat gaagcggtca aatctgtcaa
    20101 actaccagag atgaagcata tcctcctgga agtaattctg ttcgtgctgt ttccatatct
    20161 tctttgccat catcagttac ttctctgtct aaataaaatc ttacgataag tttttgtcta
    20221 atttcatcaa attctgccgt aattaatctt atattgatat gaatacacgg ttcatacctg
    20281 ctattagcca gtcaggtgta gttggatggg gattcatttt taataatatc ttcaagcaag
    20341 tgaaatgtaa gaaaagcgta gatacttgaa ggccggctat ctgattggca aaaagaaatt
    20401 tcggagataa agaatttccg tttatatttt tcataaatta aatcaacacc ttgttttaca
    20461 taaaaactaa cttcctcagg aaggatattt ataacattct ctccttttac ttttatagtt
    20521 aatggtttga tttttatgtt ttcatgaata tctgatagga ttatttctaa aaaattattt
    20581 ttatttccag tcattctggc tattttatgt atatttccat tgtgttcgat tatcatttta
    20641 ttttttccaa ggatttcctt ctatattgat agtttatcta aagatttcat ttaaaatatc
    20701 ctgactaatt tcttgtttat ataattctac aattttctgt ttctgtgtat ttgtataaaa
    20761 tttttcgttg gtatctgctt gatattttaa ttgtctcaat atttccatga tttcatattc
    20821 tgatttttca tcatattctt caatcatgta atcaacaaat tgttttagaa tatcaaaggc
    20881 ttcgtaactt tccaaaattt taatatgttt atcggcaaaa ttggacatat accattcgtc
    20941 agtgattgga ttagtattta aatcatctaa aaaaacattt aaatcatcta aaaaaacatt
    21001 tacatcatct aaaaacatga ctaatcctta aacttaaaat ctgttttctc accggttctt
    21061 gagttttcaa tgtaatgtat ttcaattttg tttacgtttt gaacacgctt aacccaaccg
    21121 tcttttgcta accaaccatt tttagtatcg gacataggag gtattctcgc aggagttttg
    21181 ccctgtgggt ttgccttaac ttcttccatt gctaattgtt cctttaaatt attaggtatt
    21241 gtacgaccgg ttgatttttc aacagattgt gtccctattt ttaatgattt gtttccccca
    21301 gccatttccc catctaaagt ctgcataggg cgggcagcag gttttttata accggtattt
    21361 ttaacccaat cccatttggt cgggttaagg tttacttttt taccgcccca aaccgtacct
    21421 gcggcctctg ctatggcaag agcagtttgg gctgttgcag ttatattcgg atgggcatca
    21481 gcccattgtc tggcggaatt aatgccgtct tttaccgcaa aagcactgtc ctgtaatagg
    21541 ctcgcggcag caagttgtgc ttccggactt aaatttccta att
//
LOCUS       DAWFAN010000016        21327 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWFAN010000016 DAWFAN010000000
VERSION     DAWFAN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21327
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.016"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..217
                     /locus_tag="WGO65_000416"
     CDS             <1..217
                     /locus_tag="WGO65_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEZ0326759.1"
                     /translation="QELEALPGIGPAKAKAIAEYRAQNGAFKSVDDLTKVKGIGPAVL
                     AKLKDQASVGAPAPKGPAKPVLPADKK"
     gene            486..1607
                     /gene="dnaJ"
                     /locus_tag="WGO65_000417"
     CDS             486..1607
                     /gene="dnaJ"
                     /locus_tag="WGO65_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ0326760.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTIPKETQTGRRMRVKGKGVKSLRSSAIGDLYCHIVVETPV
                     NLTDPQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1803..3821
                     /locus_tag="WGO65_000418"
     CDS             1803..3821
                     /locus_tag="WGO65_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ0326761.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(3865..4422)
                     /gene="rfbC"
                     /locus_tag="WGO65_000419"
     CDS             complement(3865..4422)
                     /gene="rfbC"
                     /locus_tag="WGO65_000419"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0326762.1"
                     /translation="MNIIDTALPDVKILKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPLQTA
                     PLLSPKDLAGKAWAQAEKLRLTLSR"
     gene            complement(4467..5357)
                     /gene="rfbA"
                     /locus_tag="WGO65_000420"
     CDS             complement(4467..5357)
                     /gene="rfbA"
                     /locus_tag="WGO65_000420"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0326763.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENF
                     RAVSIEEKPQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNQMYLEDGSL
                     SVQILGRGFAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVET
                     RAKPLEKTAYGQYLLRLIGK"
     gene            complement(5554..6636)
                     /gene="rfbB"
                     /locus_tag="WGO65_000421"
     CDS             complement(5554..6636)
                     /gene="rfbB"
                     /locus_tag="WGO65_000421"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0326764.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIQNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEF
                     IQTNIVGTFNLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLGGTDDLFTETAPYA
                     PSSPYAASKASSDHLVRAWQRTYGLPAIVSNCSNNYGPRQFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLE
                     ELAPEKPAGVARYEDLITFVPDRPGHDVRYAVDAAKIGRELGWKPQETFESGIRKTMQ
                     WYLDNKTWWQNVLNGSYRLERLGTGK"
     gene            complement(6637..7680)
                     /gene="galE"
                     /locus_tag="WGO65_000422"
                     /pseudo
     CDS             complement(6637..7680)
                     /gene="galE"
                     /locus_tag="WGO65_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(7673..8675)
                     /gene="dcm"
                     /locus_tag="WGO65_000423"
                     /pseudo
     CDS             complement(7673..8675)
                     /gene="dcm"
                     /locus_tag="WGO65_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(8706..10979)
                     /locus_tag="WGO65_000424"
     CDS             complement(8706..10979)
                     /locus_tag="WGO65_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ0326765.1"
                     /translation="MNITQILSQELSATTAQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPVRTMPGHRA
                     LAVLRGRNEGVLNIALKYRPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYKNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(11044..11694)
                     /locus_tag="WGO65_000425"
     CDS             complement(11044..11694)
                     /locus_tag="WGO65_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0326766.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMHQFEDMDKAYEYYNSLP"
     gene            complement(11691..12488)
                     /locus_tag="WGO65_000426"
     CDS             complement(11691..12488)
                     /locus_tag="WGO65_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0326767.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(12488..13651)
                     /gene="ctrB"
                     /locus_tag="WGO65_000427"
     CDS             complement(12488..13651)
                     /gene="ctrB"
                     /locus_tag="WGO65_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ0326768.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(13666..14841)
                     /gene="ctrA"
                     /locus_tag="WGO65_000428"
     CDS             complement(13666..14841)
                     /gene="ctrA"
                     /locus_tag="WGO65_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ0326769.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            14976..16109
                     /gene="neuC"
                     /locus_tag="WGO65_000429"
     CDS             14976..16109
                     /gene="neuC"
                     /locus_tag="WGO65_000429"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ0326770.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKCQSEKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            16113..16799
                     /gene="neuA"
                     /locus_tag="WGO65_000430"
     CDS             16113..16799
                     /gene="neuA"
                     /locus_tag="WGO65_000430"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ0326771.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            16800..17849
                     /gene="siaC"
                     /locus_tag="WGO65_000431"
     CDS             16800..17849
                     /gene="siaC"
                     /locus_tag="WGO65_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ0326772.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIS"
     gene            18001..21114
                     /locus_tag="WGO65_000432"
     CDS             18001..21114
                     /locus_tag="WGO65_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0326773.1"
                     /translation="MAVIIFVNGIRAVNGLVKSSINTANAFAEEGLDVHLINFVGNIT
                     GAEHLYPPFHLHPNVKTSSIIDLFNDIPENVSCRNTPFYSIHQQFFKAEYSAHYKHVL
                     MKIESLLSAEDSIIFTHPLQLEMYRLANNDIKSKAKLIVQIHGNYMEEIHNYEILARN
                     IDYVDYLQTVSDEMLEEMHSHFKIKKDKLVFIPNITYPISLEKKEADFFIKDDEDIDN
                     AQKFKRISIVGSIQPRKNQLDAIKIINKIKNENYILQIYGKSINKDYFELIKKYIKDN
                     KLQNRILFKGESSEQEIYENTDILIMTSESEGFPYIFMEGMVYDIPIVVYDFKYGAND
                     YSNYNENGCVFKTGDISGMAKKIIELLNNPEKYKELVQYNHNRFLKEYAKDVVMAKYF
                     TILPRSFNNVSLSSAFSRKELDEFQNITFSIEDSNDLAHIWNFELTNPAQNMNFFALV
                     GKRKFPMDAHIQGTQCTIKIAHKKTGNLLSLLLKKRNQLNLSRGYTLIAEDNSYEKYI
                     GAISNKGNFEIIANKKSSLVTINKSTLELHEIPHELHQNKLLIALPNMQTPLKITDDN
                     LIPIQASIKLEKIGNTYYPCFLPSGIFNNICLDYGEESKIINFSKYSYKYIYDSIRHI
                     EQHTDISDIIVCNVYSWELIRASVIESLMEFTGKWEKHFQTSPKIDYRFDHEGKRSMD
                     DVFSEETFIMEFPRKNGIDKKTAAFQNIPNSIVMEYPQTNGYSMRSHSLKSNVVAAKH
                     FLEKLNKIKVDIKFKKHDLANIKKMNRIIYEHLGININIEAFLKPRLEKFKREEKYFH
                     DFFKRNNFKEVIFPSTYWNPGIICAAHKQGIKVSDIQYAAITPYHPAYFKSPKSHYVA
                     DKLFLWSEYWNHELLPNPTREIGSGAAYWYALDDVRFSEKLNYDYIFLSQSRISSRLL
                     SFAIEFALKNPQLQLLFSKHPDENIDLKNRIIPDNLIISTESSIQGINESRVAVGVYS
                     TSLFEALACGKQTFVVKYPGYEIMSNEIDSGLFFAVETPEEMLEKTSPNWVAVADIEN
                     QFFGQEK"
BASE COUNT         5741 a         4857 c         4901 g         5828 t
ORIGIN      
        1 gcaggagttg gaggcgctgc cgggcatagg ccctgcgaag gcgaaggcca ttgcggaata
       61 ccgtgcgcaa aacggtgcgt tcaagtctgt agacgatttg accaaggtaa agggcatcgg
      121 ccctgcggtg ctggcgaagc tgaaggatca ggcttcggtc ggtgcgcccg cacccaaagg
      181 cccagccaaa ccagtgctgc ccgcggataa aaaataaaat agggggaagt ctgcagtcgc
      241 atcaaatgcc gtctgaacat gcgttcgggc ggcgttttta taacaaaaac acttcatggc
      301 ggttggtttt atgcctgtct aagtttttgt gtcgtgcata cctgaagatt tcagacggca
      361 tcggtttatg ctgtctgaaa agtgtattcc gtttcagttt gtaagctatg gcagtctgtt
      421 tgtcttgtgt tttgcgcaat tgcccttatt ttgagccgtg attttatttt gaattagatg
      481 aaaaaatgag taatcaagat ttttatgcga cgctgggtgt ggcaagaaca gctaccgatg
      541 atgagattaa aaaagcctac cgtaaattgg cgatgaaata ccatcccgac cgcaatcctg
      601 acaataaaga ggcggaagag aagtttaaag aagtacaaaa ggcgtatgaa actttgtccg
      661 ataaagaaaa gcgtgctatg tatgatcaat atggtcatgc ggcgtttgaa ggcggcggac
      721 aggggggctt cggagggttt ggcggatttg gcggtgcgca gggttttgac tttggggata
      781 ttttcagcca aatgtttgga ggcggttcgg ggcgcgccca gcctgattat cagggtgagg
      841 acgttcaagt cggtatcgaa atcacgcttg aagaagccgc aaaaggtgtg aagaaacgca
      901 tcaatattcc gacttatgaa gcgtgtgatg tctgtaacgg cagtggcgcg aaaccgggga
      961 catccccgga aacctgcccg acttgcaaag gttcgggtac ggtgcacatc cagcaggcga
     1021 ttttccgtat gcagcagact tgtccgacct gccacggtgc gggcaaacac attaaagaac
     1081 cttgcgtcaa atgccgtggc gcggggcgga ataaggcggt caagacggtg gaagtcaata
     1141 ttcccgccgg tatcgatgac gggcagcgta tccgtttgag cggcgaaggc gggccgggta
     1201 tgcacggtgc gcctgccggc gacctgtatg taaccgtccg cattcgggcg cataagattt
     1261 tccaacgcga cggtctggac ttgcattgcg aactgccgat tagttttgcc acggctgctc
     1321 tgggcggtga gttggaagta ccaacgttgg atggcaaggt taagcttact attcccaaag
     1381 aaacccaaac cggcaggagg atgcgtgtga agggtaaggg tgtcaaatcg ttgcgcagta
     1441 gtgcaatcgg tgatttgtac tgccatattg ttgtcgaaac gcctgtcaat ttgaccgacc
     1501 cccaaaaaga gcttttggaa gaatttgagc ggatttctac cggcttggaa aaccaaacac
     1561 cgcgcaagaa atcgttttta gacaagctgc gcgatttgtt tgattgattt taaggttcgg
     1621 aaacaagcag ccgtatcggg gaatctccct gatacggctg tttttgtttg tttaaaaata
     1681 gtttttattt tcaatggggt atggggcagg gtgggataac tgtttttaac tgttcttttt
     1741 aaaacttgac atcatggcgc gatgccaaca atatgtgaac gtctgttgtc aaaggaagaa
     1801 taatgaataa atctttatcc gggtcggtag aagaataccg cgaactgacg ctccgaggca
     1861 tgatactcgg tgcattgatc actgtaattt ttactgcgtc caacgtttac ctcggtttga
     1921 aagtcgggct gacctttgcc tcgtcgattc cggcggcggt gatttcgatg gcggttttaa
     1981 agtttttcaa aggcagcaat attttggaaa acaacatggt gcagacccaa gcctcggctg
     2041 cgggtacgct ttcgaccatc atcttcgtcc tgcccggttt gctgatggcg ggctactgga
     2101 gcggtttccc gttctggcag acgacgcttt tatgtattgc cggcgggatt ttgggggtga
     2161 ttttcaccat tcctctgcgt tacgcaatgg tggtgaaaag cgatttgcct tatccggaag
     2221 gtgtggcggc tgctgaaatt ttgaaagtgg gcggtcatga agaaggggat aaccgtcagg
     2281 gcggcagcgg catcaaagag ctggcggccg gcggtgcgtt ggcgggattg atgagctttt
     2341 gcgccggagg tctgcgcgtg attgccgaca gcgcgagtta ttggtttaaa agcggtacgg
     2401 cgattttcca gctgccgatg ggcttttcac tggcattgtt gggcgcgggc tatttggtcg
     2461 gactgacggg cggtatcgcc atcctgttgg gcatttcgat tgcttggggc attgccgtgc
     2521 cgtatttctc ctcacacatt ccgcaacctt ccgatatgga aatggcggcg tttgcgatga
     2581 agctgtggaa ggagaaagtg cgttttatcg gtgcggggac tattggcatt gcggcggttt
     2641 ggacgctgtt gatgctgctc aagccgatgg tggaaggcat gaagatgtcg ttcaagagtt
     2701 ttggcggcgg tgcgcccgct gcggaacgcg ccgaacagga tttgtcgcct aaggctatga
     2761 ttttttgggt gctggcgatg atgtttgttt taggcgtgtc gttttaccac tttatcggcg
     2821 attcgcacat tacgggcggc atggcttggc ttttggtggt cgtttgcacg cttttggctt
     2881 ccgtcatcgg ctttttggtc gccgccgcct gcggttatat ggcaggtttg gtcggctcgt
     2941 cttccagccc gatttccggc gtgggcatcg tgtccgtcgt cgttatttca ctggttttgc
     3001 tgctggtagg cgaatccgga ggtttgttgg cggatgaggc taaccgcaaa tttttgctgg
     3061 cactgacttt gttttgcggc tcggcagtaa tctgcgtggc ttcgatttcc aatgacaacc
     3121 tgcaagactt gaaaaccggc tacctgctca aagccacgcc ttggcggcag caagtcgccc
     3181 tgattatcgg ctgtatcgtt ggtgcgctgg ttatttcgcc cgtgttggaa ctgctttacg
     3241 aagcctacgg ctttaccggc gcaatgccgc gcgaaggcat ggatgcggcg caggctttgg
     3301 cagcccctca agcgactttg atgacgacca tcgcgtcggg cattttcgcc cacaacctcg
     3361 aatgggtcta catctttacc ggcatcgcga ttggcgcggt gttgattgcc gccgatttgg
     3421 tgttgaaaaa atcatcaggc ggcaaacttg ccctgcccgt ccttgcggtc ggtatgggta
     3481 tttatctgcc gccgtctgtc aatatgccca tcgtggcagg cgcggtgttg gcggcggtgt
     3541 tgaaacacat catcggtaaa aaagcggaaa accgtgaagg ccgtctgaaa aacgccgagc
     3601 gcatcggaac cttgttctcc gccggcctga ttgtcggtga aagcctgatc ggtgtgatta
     3661 tggcgtttat tattgccttc tccgtgacca acggcggctc ggatgcgccg cttgcgttga
     3721 atctgcaaaa ctgggatgcc gccgcctcat ggttgggctt ggcgttcttc gttaccggga
     3781 tgtttttctt tgcacagcgc gtactgaagg cgggtaggta gcctgtcgga aaaaatgccg
     3841 tctgaaacgt tcagacggca ttttttatcg ggaaagcgta aggcggagct tttcggcttg
     3901 cgcccacgct ttaccggcaa gatctttggg cgacagcagc ggcgcggttt gaagcggcca
     3961 gcctatgccg actgtcgggt cgttccatat taaaacctgt tcggcttcag gcttgtaata
     4021 gtccgtgcat ttgtacacga cttccgctgc gtcgcccaaa acgcaaaacc cgtgcgcgaa
     4081 gccttcgggt atccaaagct ggcattggtt ttgcgcggac agggttgcgc ccgcccattt
     4141 gccgaaagtc ggcgaacctt cgcgcatatc gacggccacg tcgaacactt cgccgacaac
     4201 tacgcgtacg agtttgcctt gtgtgttttc ggtttggtag tgcaggccgc gcaatacgcc
     4261 tttgccggat ttggagtggt tttcctgcac aaaggttcgg tcggcaatat tttctttgaa
     4321 ccacccgtcg cggaaggttt ccataaaaaa gccgcgcccg tctgtgaaga cttgcggttt
     4381 taaaattttt acgtcgggga gggcggtgtc gatgatgttc atgattttct ctgtatgccg
     4441 tctgaagaaa gtggttcaga cggcatttat ttgccgatta ggcgcagcag gtattgcccg
     4501 taggcggttt tttccaaagg ctttgcccgt gtttccacat cttttttcgt cagccaaccg
     4561 ttgcgccagg cgatttcttc gaggcaggcg atgtgcaggt tttggatatt ttgcacggtt
     4621 tggacgaatg aagcggcttc gtgcaggctc tcgtgggtgc cggtgtccaa ccatgcgaaa
     4681 ccgcgtccta atatttggac ggacaatgaa ccgtcttcca aatacatttg gttgaggtcg
     4741 gaaatttcca attcgccgcg tgcggacggt ttgagctgtt tggcgaactc gacggcgcgg
     4801 ttgtcgtaga aatacaagcc ggttaccgcc caatcggatt tgggccgttg cggtttttct
     4861 tcgatggaaa cggcgcggaa gttttcgtta aattcaacca cgccgaaacg ttcggggttt
     4921 ttgacctgat aagcaaacac ggttgcgccg tgcgtttgcg ctgccgcctg tttcaatgtt
     4981 tgcgctgccg cctgtttcaa tgtttgcgta aacgactgac cgtaaaaaat attgtcgccc
     5041 aaaaccaagc aaacattgtc gttgccgata aattcttcgc cgatgataaa tgcctgtgcc
     5101 aagccgtccg gactgggttg cacggcataa ctgatggaaa tgccgaaatc gctgccgtcg
     5161 ccaagcaggc gtttgaaaga ggcgttgtct tcaggcgcgg taatcaccaa aatatcgcgg
     5221 attcctgcca gcatcaaaac cgacaagggg taataaatca tcggtttgtc gtacacgggc
     5281 aggagctgtt tggatacgcc gcgcgtgatg gggtagaggc gcgtgccgct gccgccggca
     5341 aggatgatgc ctttcatcgt atttcctttt cggttgaaac cccgcccttt agggcggtag
     5401 aatcagactt tatttgggag ggggtaaccc cttccaaatc aggacggcac atagggcgac
     5461 gctttatgtg ccgtcctgtg tgttgaaaca tcttttcttt cttcctttgc gataggtttt
     5521 cagacggcat tgcgtcggga tgccgtctga aaactatttg cccgtaccta aacgttctag
     5581 acgatagcta ccgttcaata cattttgcca ccaggttttg ttgtccaaat accattgcat
     5641 tgttttgcgg atgccggatt cgaatgtttc ttgaggtttc cagcccagct ccctaccgat
     5701 tttggctgcg tcgacggcgt agcgcacatc gtggccgggg cggtctggga caaaggtaat
     5761 caaatcttca taccgtgcca caccggccgg tttttcggga gcgagttctt ccagcagggc
     5821 gcagatggtt ttgacgactt caatattggc tttttcattg tggccgccga tattgtaggt
     5881 ttcgccgaca acaccttcgg taacaacctg atacagtgcg cgcgcgtggt cttcgacaaa
     5941 cagccagtcg cggatttgca taccgtcgcc gtaaacgggc agcggtttgc cgtcgagcgc
     6001 gttcagaatc atcagcggga tgagtttttc ggggaattgt cggggcccgt aattattgga
     6061 acagttgctg acaatggcgg gcagcccgta agtccgttgc cacgcgcgga caagatgatc
     6121 ggaagatgct ttagacgcgg cataaggact ggacggcgcg tatggcgcgg tttcggtaaa
     6181 caaatcgtcc gtgccgccta aatcgccata gacttcatcg gtggaaatat ggtggaaacg
     6241 gaaggcttcg cgcttttcag acggcatttg ttgccagtag gcgcgggctg cttcaagcag
     6301 attgaatgtg ccgacgatat tggtttggat aaactcgcct gccgaaccga tagagcggtc
     6361 gacatggctt tccgccgcca agtgcatcac ggcatcgggc cggtgttgtg cgaatacgcg
     6421 gtcgagttcg gcgcggtcgc aaatgtccac ttgctcgaaa gcatagcggg gattatcggc
     6481 aatgtcggtc agcgattcga gattgcccgc gtaggtcagt ttgtcgaggt tgacgacgga
     6541 atcttgggtg ttttgaatga tatggcggac aacggcagag ccgataaagc ccgcgccgcc
     6601 ggtaacgagg atggtttttt tgtttgcggt ttgcatttaa tccccatatc cgttggggtt
     6661 gcggctgacc caacgccacg aatcctccat catttgcgca aggctgcgtt tggtttccca
     6721 acctgtctct tgtttggtat gggacgggtc ggcaaaggaa cacgccaagt ctccggcgcg
     6781 tcggggttgg atttgatagg gaatatttaa gccggatgcg gcttcaaagg cacggatgat
     6841 ttccaaaacc gaataggcgc gccccgaacc caagttgaac aaatgtacgc cggcaacacc
     6901 gcctttcgcc ttcattgccg cgatatgccc ttctgccaaa tccatcacat gaatatagtc
     6961 gcgcattccc gtaccgtccg gggtcggata atcgccgccg aataccgaca gttgcggcag
     7021 cctgcccgaa gccacttggc agatataggg caaaaggttg ttgggaatac cgttgggctg
     7081 ttcaccgatc aagccgcttt cgtgcgcccc aatcgggttg aaatagcgca acaaaatcac
     7141 gctccaacgc ggatcggcct tttggatgtc ggttaacatc cgttccacca tcgctttgga
     7201 tgcgccataa ggattagcgg tatcgcccgg gcgcatatct tccgtatagg gcattttacg
     7261 tatcatggcg cgacgcatta aagtaaacgc caaagcgcgt ttatactggt tatccagttt
     7321 ccctatattt ttacgttaca aatcaagttg ttggtattca tcgtgaaaat agaatttttc
     7381 ggcataacgc tgactcataa aaccggcatg cgggctgcct gaaaaaatca ttgcgacatc
     7441 atcgtcattt aatgtttcat ggttgttttc tattaatgct aaagcaattt ggtaattaat
     7501 tgccaaaata tcgttagtaa tcacacgata gccgcgtttt ttggcttcgt aggcaaagga
     7561 acagcctcca ctaaatacat ctgcaactgt atctacatca gacggaagct ggtcacaaat
     7621 ccaggaagct attttctctt tattaccgat atagttaatt ttcggatatt gcttattcat
     7681 tttcactcat ttttaaaata ctttcagcaa tagcttttgc caataaaggc ggtacggcgt
     7741 taccaacctg ctgctgctgg gcaattttgc tgccgcaaaa aataaaatta tcagggaaag
     7801 attgtaaggc agctaattca cgaacggtta acgcccgatt ctgttcatag tgaaaaactt
     7861 tgcgcatatc tcctgtaata caaacggctg gttttgttgc tgttgtaacg gatgtattta
     7921 cggatatcac ctgttttcgg acgtaatggt tcaggaatat cgttacggtt acctccattt
     7981 ttaacaaatg ccattttttc taacatttgt gccgaatgat tcatagcttc atgatttgca
     8041 acgtgtggat tgctttcgcc agcagccagt tttggaaaat gtcctattgc tgatccaaca
     8101 gtctgatggg aaatctgcaa aggttcggga aaggaaattt tgcctttatc cctcctcccg
     8161 ataaatatca ctcggctacg tatctgagga acaccgaaat cggctgcact cagtatctta
     8221 cattccaccg aataaccgat attctgaaat gcttgaataa tctcaatacg tgttttacct
     8281 gaattgtgtg tatagagtcg cgctacattt tccataacaa aaaaatatgg ttggacaatt
     8341 ttaactattc ggacaaactc tttaaataaa tggttgcgtg ggtcatctgt aaatgtccgt
     8401 ccaatctttc ctgccatact aaaaccttga caaggtggtc ctccaataat caaatcaact
     8461 gcttgtccgt taagacaatt gattaaatct tgttcggtta gtgtggttaa atctttttgc
     8521 agtaattgat gatgggggaa gttggtacgg taagtctgac aataatcaga ctccatttca
     8581 acagaaagca attgttggaa tccggcttgt tcaaaaccca aggataggcc tcctgctcct
     8641 gagaaaaggt caatataggt aagtggtgtg tgcataagtc aaaatccata aactcttcga
     8701 ttattttacc gcttcagctt cgcaaacgca tccgccatcg ccgaattggc tggggcgcgg
     8761 tcgttgcgtt ggggtttgcg gccggctgtc cgttcgcggc tgcggttttc agacggcatt
     8821 ttatgttttg cgccgcccgg ttcgtcatcc aagcgcatgg tcagcgcgat gcgtttgcgt
     8881 gcagcatcga cttccagcac tttcactttc accacgtcgc cagctttcac cacttcgcgc
     8941 gggtcttgga cgaacttgtt ggacagggcg gagatgtgca ccaagccgtc ctgatggacg
     9001 ccgatgtcca cgaacgcgcc gaagttggcg acgttggaaa ccacgccttc gagtatcata
     9061 ccgacttgca agtcgctgat ttcgtggata ccttcggcaa acgatgccgt ctgaaactcg
     9121 ccgcgcggat cacggccggg tttttccagt tcggacagga tgtccaaaat ggtcggcagg
     9181 ccgaagcgtt cgtcggtgaa gtcggacgct ttgatttgct tcacgcgctc gcggttgccg
     9241 atgagttcgg cggcgctaat gccttgttgc gccagcattt tggcgacgac gggataggct
     9301 tcggggtgga cggcgctcgc gtccaacggc tctttgccgc cgttaatccg caaaaagccc
     9361 gccgcctgct cgaaggtttt ttcgcccaaa cgcggtactt tcagcaattt tttgcggctg
     9421 tcgaacgcgc cgttttcatc gcggtaggcg acaatgtttt gggcaagggt ttgattcaaa
     9481 ccggaaattc gcgccaagag cggggcggag gcggtgttca cgtccacgcc gacggcgttc
     9541 acgcagtctt cgaccaccgc gtccagcgat ttggcgagct ggctttggtt cacgtcgtgc
     9601 tgatactgcc ccacgccgat ggatttgggg tcgattttga ccaactcggc aagcgggtct
     9661 tgtagcctgc gggcgatgga caccgcgccg cgcagggaaa cgtccaagtc ggggaactcg
     9721 cgcgccgcca gttcggacgc ggaataaatc gacgcgccgg cttcggacac gacgatttta
     9781 tgcagcccca ttttcgacat ttcacgcacc agttcgcccg cgattttgtc ggtttcacgg
     9841 ctggcggtgc cgttaccgat ggcgatgagc ttcacgccgt gctgtttaat caggcgcgac
     9901 agcgttgcca acatattgtt ttcttgatgc aaatagacga tgacggtatc cagcagcttg
     9961 cccgtgtcgt ccaccacggc acatttcacg ccgttcttgt atccagggtc tagacccaaa
    10021 gtggtcagcc gtccggcggg tgcggcgagc agcaagtctt tgagatttcg ggcaaacacg
    10081 gtaatcgcgt cggtgtcggc ggcttctttc aaacgattga gagcttcgag ttccaacgac
    10141 aaaaagattt ttgcgcgcca agtcagacgc acggtgtcgc gcagccattt gtggccgtct
    10201 gaaaccttga aacggcgggc gatgatttgc tcgtattcgc tttgctgcgt aatcggcgtg
    10261 tcgtcgggcc ggtatttgag cgcgatgttc aacacgcctt cgttgcggcc gcgcaaaacc
    10321 gccaacgcgc ggtggccggg catagtgcgg acgggttcgc ggtggtcgaa ataatcgctg
    10381 aatttttcgc cttcggtttc tttgcctcca acaacttgcg cgtggatttc ggcttcgttc
    10441 cacagcttgt cgcgcagcgt gccgatgagt tccgcgtctt cggcaaactg ctccatcaga
    10501 atcgcacgcg cgccgtccaa cgcggctttg gcatcgggga cgttttcgtt caggtagcct
    10561 tgtgcggcgg cttccacgtc ctgcgactgc tcggcaagca acacgtccgc cagcggctgc
    10621 aaaccgtgtt cgcgcgcgat ttgcgctttg gtgcggcgtt tgggtttgta gggcagatac
    10681 aggtcttcca gcgcggtttt gttatcggcg gcttcgattt gcgccctgag gtcgtctgaa
    10741 agcttgcctt gctcttcaat gctttttaaa acaacggctt tgcgctcttc caactcgcgc
    10801 aggtattgca gccgctcggc aagccggcgc agctgcgtat cgtccaaccc gcccgtggct
    10861 tccttgcggt agcgggcgat aaagggcacg gttgcgccgt cgtccaaaag ctcgatagcg
    10921 gcattgattt gcgcggtagt cgcggaaagt tcttgggaaa gaatttgagt aatgttcatc
    10981 aataaaattc caacggacag gccgtctgaa atttcagacg gcctgattta aaaaacaatc
    11041 gctttaaggt agcgaattat aatattcgta ggctttgtcc atatcttcaa actggtgcat
    11101 atgccctttt tccagcacca tcgcattatc gcaatattgc ttcatggcgc tgtggctgtg
    11161 cgacaccaag atgatggaac ggtctttgcg cttttcaaac aactcgtact tacatttatc
    11221 ggcaaaacgc gagtcaccaa ctgcaatcac ttcgtcaatc aaatagcaat caaactctac
    11281 cgccaacgac aaagcaaatg ccaaacgcgc cttcatacct gaagaataac gcttgaccgg
    11341 ctcgtaaaga tattgcccca actcggaaaa ctcttcggta aagttcttca cataatcgat
    11401 atcgacattg taaatccggc agatgaaacg caaattgtcc ataccggtca gactgccttg
    11461 aaacgcaccg gagaatgcca aaggccaaga aatactcatt gtccgcttga tttcacccgt
    11521 ggtcggcggc tcaacgccac tgatcaaacg gatgagcgtc gatttacctg caccgttgcg
    11581 gccgagaata ccgattttct cgcccttctc cattttgaag ctaatatcgt gcaagactgt
    11641 ccgccaacct tggcgggtca gatagcgttt ggaaacgtgt tcaactgaaa tcattgcggc
    11701 tcaacccctt tgctgaatct cgccaccacc gccaaaccaa gcagcagcaa aaccaagtta
    11761 cacaacaaaa tataccaagg attttcgtaa gtcgtgacgc tgtcgccaaa atatccggca
    11821 cggaacattt ctgtgccatg caccatcggg atcatcaatg catactcttg cactttcgga
    11881 ggcaaattat gtacaaaaaa gaatgcaccc gataacggca tcatcacaaa agtcaatgtg
    11941 ccccaaatct tgccaaacgg ctcgaaatta aaggcaatcg aacaaatcac caaacccaaa
    12001 ccaatcgcaa aaaaagccat caaaagccaa gccatcagca tataaaacat atctgccggc
    12061 atttcaatcc agccaatcgc aatcaatacc gccataatca caatctgcgc aatggttgca
    12121 ccagcaattt ccaaaatcat gcgcgccaag atggtatcca aaactcttac attgcggtga
    12181 taaagcaagc tggcatttga agaaatcgac ccaactgccc gttttgaggc attacgccac
    12241 atcatcaaca tcggatagcc agtaatcgca aatgcgacaa tattcaaagt tgaatatcgg
    12301 tctgccttta aaaatttcca catcaagacg ataacgaatg tcatcagcaa cggctcaaca
    12361 aacagccata aaaagccaat attattgcga ccgtaacggg tgataatttc ccgcatcaaa
    12421 agcgcaccga ttacgcgcct ttgaatggct aaagattccc aaaatgatgt tttatgcaag
    12481 gctttcatca gtttttatgc tcacgaatgc tggcagtcaa caggctcaaa ataccataaa
    12541 ccatcaagcc gataatcaga gtggcaacaa tgttgtataa ccgtttaggc tcatgtgcca
    12601 aatccggcag gctcggttgc gagatcactt ccaaataaag ctgctgacgg tctgcttcaa
    12661 ccttggcact ttccaaagaa gtcatggcgg ctgccaactg ctgctctgcc aactggtttt
    12721 ccaaatacac acgctgatat tcggcagcct gattagacaa cgaagaatgc ccaccgcccg
    12781 aaatggcacg taactgttgg tcaatttctt tacgcaagct ctgctcacgc gcctgcaaac
    12841 ccggaatctg cggattctcc ggagtgactg ctttcacctg atccagctgg gtttgaatca
    12901 caatcaattc atcttgcagc ttggaaacca accccatttg cacttccgat tgcgctttca
    12961 aatcaaaaac gccattggca atccggtaat ccgtcagatt ctgagaggct tcctttaccc
    13021 gctctgccgc cgttttcact acttcttccg catagcgcac cgtatcagca cgtgcacgat
    13081 cgttcaactg gttaatcaat gcttcacctt gttttaacaa agcctcattg attttcttag
    13141 attccagcgc atcaaaggaa gttacattca acgtggaaat acccgaaacc gtatcaaaat
    13201 tgatcatcac ctgattttta tagtattgat aaaaagcctc ttcctcgcca cggaacccaa
    13261 acccattaaa gcggctgaac gcatcacctt tggtttcata aaactcacgc accggcaaga
    13321 ttttacgcag ttcatccaaa gacgagcgcg aacgcatata ctccccaacc gtgtaaatat
    13381 catcttgcgc acgggcaaaa cctgtgccct gcaaaatggc acccaggcca ttgagagaag
    13441 attggctttt aggcgagcgc accacaaagc tcgattgcga cgtaaaacga tcggaagcga
    13501 agaagccgaa atacaccaac gaaattaccg tagggataat caccgttacc caaaataaag
    13561 ggcttagctt tttaatccaa cttttctttt tcggcttttt acgctcggct ttggtttcag
    13621 tggcaactgc cacaggaagt tgctcagaca tcttaattac tcacattaat tagttaaatt
    13681 attaatactg ttcgcgccac tggtaaccgg cgagaacaca aacgacaaga atttctgcac
    13741 ttcagccaac ggcgcattcg acacatacaa tacatcttta ttcttcacag gaaagcgctg
    13801 catagaaaat agcgaatgcg catcagccat attcacacga tataccgttg gaatctctgc
    13861 ctcactgcca taaccttgag caatccattt atcctgacgt tctgccggca attccaccaa
    13921 tggcgtatag cggaacacaa acacaccacg cgcatcagaa cggcgatctt gcaaaccgcc
    13981 catacggcca atggcttcag aaagcgataa gcctctggct gaaaaaccga tttcttgtgt
    14041 tctccccacc gcacccatag acgtaaaggt atagggattg gtaatcatgg taaccacatc
    14101 accgcgacgc agcaaaatat tttgtcgcgg atttgcaact aaatcttcca aggcaacagt
    14161 tcgtactaca ttgccacgtg tcagctgcac attcgtatcc tgcacatttg ccgttgaacc
    14221 acctaccgca gccaccgcat ccaacacacg ctcaccggct gccgtcagcg gcatacgcac
    14281 actattccca gcacgaatca ccgacacatt cgccgcatta ttctgcacca aacgcaccat
    14341 cacttgtggc tgattggcca tttttttcag gcggccttta ataatttcct gaacctgacc
    14401 aggcgtttta ccgaccaccg aaatatcgcc aacaaacggc acagaaaccg taccacgtgc
    14461 cgtgaccaac tgctctggca acttagtttg atgcgcacta cccgagccca tcgaagaaag
    14521 gccaccacca aacaatactg ccggcggcgc ttcccaaatc ataatatcca atacatcacc
    14581 aatatttagc gtaccagccg aagcataacc atcgccaaac tgagtgaatg actgatttat
    14641 ctgagcctta tataataact gagcaaccgt atgattcaca tcaatcagct ccacttcagg
    14701 aatttgaact tcagattgtt gccctaaaga gacaattttt tttgcgctgg ggcctgatga
    14761 aggaatcgca gagcatccta caattaaact tccacacaat aataatactg cgtgacgaat
    14821 ataaaatttc actttaaaca caagccaaat cctaatataa ttataaatgg cctaattata
    14881 gcacttaatc gaaataaatt tatgagtacg tagagtataa ttagtattct tctttccaac
    14941 ttccttatac ttatatactt atagattcta aaatcatgaa aagaattctt tgcattacag
    15001 gtaccagagc cgacttcggc aagctaaaac ctttattagc ctatattgaa aatcacccag
    15061 accttgaatt gcatttgatt gtaactggta tgcatatgat gaaaacatat ggcagaacct
    15121 gcaaggaagt aactcgagaa aactatcaac atacatatct gttttcaaat caaatccaag
    15181 gtgaaccaat gggtgccgtt ttaggcaata ccattacgtt tatctctcgt ctatctgatg
    15241 aaattgaacc tgatatggtc atgattcacg gcgaccgttt agaagcacta gcaggcgcaa
    15301 ctgtaggtgc attaagcagc cgtttagttt gccatatcga aggtggtgaa ctatctggta
    15361 cagtagatga ctccattcgt cattctatta gtaaactttc tcatatccac ttggtagcaa
    15421 atgaacaagc tgtcactcgc ctagtgcaaa tgggagaaaa aagaaagcat attcacatca
    15481 tcggctcccc cgatttagat gttatggcct cttccaccct cccatcctta gaagaagtca
    15541 aagaatatta cggtttacca tacgaaaatt atggtatttc tatgtttcac cccgtgacta
    15601 cagaagcaca tttaatgcca caatatgcgg cccaatattt caaagcatta gaattaagtg
    15661 gccaaaatat cattagcatt taccctaaca acgacacggg tactgaaagt attttacaag
    15721 agttattaaa atgtcaatct gaaaaattta tcgcatttcc atctatacga tttgaatact
    15781 ttttagtctt attgaaacat gctaaattta tggtcggcaa ctcaagtgca ggtattagag
    15841 aagctcctct ctacggtgtc ccttcaattg atgttggtac acgccaaagt aaccgccata
    15901 tgggaaaatc tattattcat acagattatg aaactaaaaa tatctttgat gcgattcaac
    15961 aagcatgcag tttaggcaaa tttgaagcag atgatacctt taatggcgga gatactcgca
    16021 ccagcacaga aagatttgct gaagtaatca acaatcctga aacgtggaat gtttctgctc
    16081 aaaaacgttt tatcgatttg aatctttaaa ttatggaaaa acaaaatatt gcggttatac
    16141 ttgcgcgcca aaactccaaa ggattgccat taaaaaatct ccggaaaatg aatggcatat
    16201 cattacttgg tcatacaatt aatgctgcta tatcatcaaa gtgttttgac cgcataattg
    16261 tttcgactga tggcgggtta attgcagaag aagctaaaaa tttcggtgtc gaagtcgtcc
    16321 tacgccctgc agagctggcc tccgatacag ccagctctat ttcaggtgta atacatgctt
    16381 tagaaacaat tggcagtaat tccggcacag taaccctatt acaaccaacc agtccattac
    16441 gcacaggggc tcatattcgt gaagcttttt ctctatttga tgagaaaata aaaggatccg
    16501 ttgtctctgc atgcccaatg gagcatcatc cactaaaaac cctgcttcaa atcaataatg
    16561 gcgaatatgc ccccatgcgc catctaagcg atttggagca gcctcgccaa caattacctc
    16621 aggcatttag gcctaatggt gcaatttaca ttaatgatac tgcttcacta attgcaaata
    16681 attgtttttt tatcgcccca accaaacttt atattatgtc tcatcaagac tctatcgata
    16741 ttgatactga gcttgattta caacaggcag aaaacattct taatcacaag gaaagctaaa
    16801 tgcaaaacaa caacgaattt aaaattggta atcgttcagt aggttacaac cacgaaccat
    16861 tgattatctg tgaaatcggc atcaatcatg aaggctcttt aaaaacagct tttgaaatgg
    16921 ttgatgctgc ctataatgca ggcgctgaag ttgttaaaca tcaaacacac atcgttgaag
    16981 acgaaatgtc tgatgaggcc aaacaagtca ttccaggcaa tgcagatgtc tctatttatg
    17041 aaattatgga acgttgcgcc ctgaatgaag aagatgagat taaattaaaa gaatacgtag
    17101 agagtaaggg tatgattttt atcagtactc ctttctctcg tgcagctgct ttacgattac
    17161 aacgtatgga tattccagca tataaaatcg gctctggcga atgtaataac tacccattaa
    17221 ttaaactggt ggcctctttt ggtaagccta ttattctctc taccggcatg aattctattg
    17281 aaagcatcaa aaagtcggta gaaattattc gagaagcagg ggtaccttat gctttgcttc
    17341 actgtaccaa catctaccca accccttacg aagatgttcg attgggtggt atgaacgatt
    17401 tatctgaagc ctttccagac gcaatcattg gcctgtctga ccatacctta gataactatg
    17461 cttgcttagg agcagtagct ttaggcggtt cgattttaga gcgtcacttt actgaccgca
    17521 tggatcgccc aggtccggat attgtatgct ctatgaatcc ggatactttt aaagagctca
    17581 agcaaggcgc tcatgcttta aaattggcac gcggcggcaa aaaagacacg attatcgcgg
    17641 gagaaaagcc aactaaagat ttcgcctttg catctgtcgt agcagataaa gacattaaaa
    17701 aaggagaact gttgtccgga gataacctat gggttaaacg cccaggcaat ggagacttca
    17761 gcgtcaacga atatgaaaca ttatttggta aggtcgctgc ttgcaatatt cgcaaaggtg
    17821 ctcaaatcaa aaaaactgat attagttaat aataaaatag attaagctat tcttaaattc
    17881 agaatattgc ttatctatat taaaaatttc taatttttaa ggttctgatt gaaatcagaa
    17941 ccttatttca actattactt tttactcata atcgaattat atactttagg actttataat
    18001 atggctgtta ttatatttgt taacggaatt cgggctgtaa atggccttgt taaatcatct
    18061 atcaatactg caaacgcttt tgctgaagaa ggactggatg ttcatttaat taattttgtt
    18121 ggcaatatta ctggagcaga gcatttatac cccccattcc acttacatcc caatgtcaaa
    18181 acctccagca tcatagattt atttaatgac attccagaaa atgttagctg ccgaaatact
    18241 cctttttatt ctattcatca acaattcttc aaagctgaat atagtgccca ctataagcat
    18301 gttttgatga aaattgaatc tttattatct gcagaagata gcattatctt cactcatcct
    18361 cttcaactgg aaatgtatcg tttagcgaat aatgatatca agtcaaaagc caaactaatt
    18421 gtacaaattc atggtaatta tatggaagaa atccataact atgaaatttt ggcacgaaat
    18481 atcgattatg ttgactatct tcaaacggta tctgatgaaa tgctggaaga aatgcattcc
    18541 catttcaaaa tcaaaaaaga caaattagtt tttattccaa acatcactta tcccatttca
    18601 ttagaaaaaa aagaagctga tttctttatt aaggatgatg aagacatcga taatgctcag
    18661 aaatttaaac gtatctctat tgttggcagc attcagccaa gaaaaaacca attggatgcc
    18721 attaaaatca tcaataaaat taaaaatgaa aattacattt tacagatata tggcaaatct
    18781 attaataaag attactttga attaattaaa aaatatatta aagacaataa gttacaaaac
    18841 cgtatcttat tcaaaggtga atcttccgag caggaaattt atgaaaatac agatatcctg
    18901 atcatgacat cagaaagtga gggatttcca tatatattta tggaaggcat ggtgtatgat
    18961 attccaatcg ttgtatatga ttttaaatat ggagcgaatg attacagtaa ctataatgaa
    19021 aatggttgtg tttttaaaac tggtgatatt tctggaatgg caaaaaaaat aattgagcta
    19081 ttaaataacc cagaaaaata taaagaatta gttcaatata atcacaatcg cttcttaaaa
    19141 gaatatgcaa aagatgtggt tatggctaaa tatttcacta ttcttccgcg cagctttaat
    19201 aacgtatcat tatcgtctgc tttcagccga aaagaattgg acgaattcca aaatattact
    19261 ttttctattg aagattctaa tgatttagct catatttgga atttcgagct aaccaatcct
    19321 gcacaaaata tgaatttttt tgctttagtt ggcaagcgaa aatttccaat ggatgctcat
    19381 atccaaggaa cacagtgtac gattaagata gctcataaaa agacagggaa tttattgtcg
    19441 cttttactaa aaaaacgaaa tcagttgaat ttatcaaggg gatatacctt aattgcagaa
    19501 gataatagct atgaaaaata tattggagca atatctaata aaggtaactt tgaaattatt
    19561 gcaaataaaa agagctcatt agttactata aacaaaagta ccttagagtt gcatgagatt
    19621 ccccatgaac tacatcagaa taaattactg attgctttac ccaacatgca aacgcctcta
    19681 aaaattactg atgataattt aatacctatc caagcctcca taaaattaga aaagattgga
    19741 aatacttatt acccatgttt cttgccatct ggcatattta ataatatctg cttagattac
    19801 ggtgaagaat ccaaaattat taattttagt aaatattctt ataaatatat ctatgactca
    19861 attcgtcata ttgagcaaca tacagatata tcggatatta tcgtttgcaa tgtttattct
    19921 tgggaactta ttcgtgcctc agttattgag agccttatgg aatttaccgg aaaatgggaa
    19981 aaacactttc agacttctcc taaaattgat tatcgatttg atcatgaagg taagcgttcg
    20041 atggatgatg tcttttcaga agaaacattt attatggaat ttccgcgtaa aaatggtata
    20101 gataagaaaa cagcagcctt ccaaaatata ccaaacagta ttgtaatgga gtatccgcag
    20161 accaatggtt acagtatgcg cagtcattca ctgaaaagta atgtagttgc ggcaaaacat
    20221 tttcttgaaa aattaaataa aattaaggta gatattaaat ttaaaaagca tgaccttgca
    20281 aacatcaaaa aaatgaatcg aattatttat gagcatttag gcattaacat aaatatcgaa
    20341 gcatttctaa aaccacgatt agaaaaattt aagcgtgaag aaaaatattt tcatgatttc
    20401 ttcaaaagaa ataattttaa agaggtaatt tttccaagca cttattggaa tccaggtatt
    20461 atttgtgctg cacataaaca aggtattaag gtatctgata ttcaatatgc tgccattact
    20521 ccttatcatc ctgcgtattt taaatcacca aaatcacatt acgttgctga taaattgttc
    20581 ttatggtctg aatattggaa tcatgagctt ttaccaaatc caacacgaga gattggttct
    20641 ggtgccgcat attggtatgc attagatgat gtgagatttt cagaaaaact gaattatgac
    20701 tatatctttc tatctcaaag taggatttct tcgcgcttgc ttagttttgc aattgagttt
    20761 gcattaaaaa atcctcaact acagctttta ttttctaagc atccagatga aaatatagat
    20821 ttaaagaaca gaattattcc tgataatctt ataatctcca cggaatcttc tatacaaggc
    20881 atcaatgaat ctcgcgttgc tgtaggtgtt tattcaacta gcttatttga ggcattagca
    20941 tgcggcaaac aaacttttgt tgttaaatat ccgggatatg aaattatgtc aaatgaaata
    21001 gattcagggt tattctttgc agtagaaaca cctgaagaaa tgcttgagaa aacaagcccg
    21061 aattgggtgg ctgtggcaga tattgaaaac cagttttttg gccaagaaaa ataattttta
    21121 agttaatctt taaaagtgca atatttttct tattttaaat attgcacttt attcttttaa
    21181 tcataggaac tcacatgtat tctgaacagg gaattaataa tactattaac atatctacta
    21241 catctttaac caatgccacg caactaactg tgatcgggaa taataactct gtatatatag
    21301 gtaataattg taaaatcgtc agctcca
//
LOCUS       DAWFAN010000017        20989 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWFAN010000017 DAWFAN010000000
VERSION     DAWFAN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.017"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(88..861)
                     /locus_tag="WGO65_000433"
     CDS             complement(88..861)
                     /locus_tag="WGO65_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ0326774.1"
                     /translation="MSVNILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
     gene            complement(960..1583)
                     /gene="rsmG"
                     /locus_tag="WGO65_000434"
     CDS             complement(960..1583)
                     /gene="rsmG"
                     /locus_tag="WGO65_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ0326775.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQGEIDRLPQDVCVEKVQRLDVPGLDAERHIAILRKR"
     gene            complement(1679..1960)
                     /locus_tag="WGO65_000435"
     CDS             complement(1679..1960)
                     /locus_tag="WGO65_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326776.1"
                     /translation="MNLVVSAGLTKLAYKKMMRRHSRYALFLSGVAACAAAAVAWIFE
                     LLLGNAALGGLRGEAVSEYAFAVWLVAMLTLPKHLTRAPVQPVVFHRKK"
     gene            complement(1957..2151)
                     /locus_tag="WGO65_000436"
     CDS             complement(1957..2151)
                     /locus_tag="WGO65_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HEZ0326777.1"
                     /translation="MFVNEKDPYAALFAGLVFLTLPFALAVHDAFALAFGRAGLLVSV
                     SAGVAVGTVLFGLCSVCLRF"
     gene            2258..2333
                     /locus_tag="WGO65_000437"
     tRNA            2258..2333
                     /locus_tag="WGO65_000437"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2291..2293,aa:Thr,seq:cgt)
     gene            2407..3534
                     /locus_tag="WGO65_000438"
     CDS             2407..3534
                     /locus_tag="WGO65_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ0326778.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            3715..4272
                     /gene="frr"
                     /locus_tag="WGO65_000439"
     CDS             3715..4272
                     /gene="frr"
                     /locus_tag="WGO65_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ0326779.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            4328..5074
                     /locus_tag="WGO65_000440"
     CDS             4328..5074
                     /locus_tag="WGO65_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ0326780.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFSRQILKGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAKGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            5077..5874
                     /locus_tag="WGO65_000441"
     CDS             5077..5874
                     /locus_tag="WGO65_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ0326781.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLSGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            5879..7114
                     /locus_tag="WGO65_000442"
     CDS             5879..7114
                     /locus_tag="WGO65_000442"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ0326782.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSLHP
                     EKFRVFALAGHKQVEKLAVQCRTFRPEYAVVADAEHAARLEALLKGDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPD
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            7149..8489
                     /gene="rseP"
                     /locus_tag="WGO65_000443"
     CDS             7149..8489
                     /gene="rseP"
                     /locus_tag="WGO65_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ0326783.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            8546..10939
                     /gene="bamA"
                     /locus_tag="WGO65_000444"
     CDS             8546..10939
                     /gene="bamA"
                     /locus_tag="WGO65_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ0326784.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGERFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            11005..11505
                     /locus_tag="WGO65_000445"
     CDS             11005..11505
                     /locus_tag="WGO65_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ0326785.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVATFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            11538..12584
                     /gene="lpxD"
                     /locus_tag="WGO65_000446"
     CDS             11538..12584
                     /gene="lpxD"
                     /locus_tag="WGO65_000446"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ0326786.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            12618..13067
                     /gene="fabZ"
                     /locus_tag="WGO65_000447"
     CDS             12618..13067
                     /gene="fabZ"
                     /locus_tag="WGO65_000447"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ0326787.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            13154..13930
                     /gene="lpxA"
                     /locus_tag="WGO65_000448"
     CDS             13154..13930
                     /gene="lpxA"
                     /locus_tag="WGO65_000448"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ0326788.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRHAETQAELAVFRDFFAQSARGIIR"
     gene            14288..15679
                     /locus_tag="WGO65_000449"
     CDS             14288..15679
                     /locus_tag="WGO65_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0326789.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPLMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFMPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMALA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAIVVLGGIKGIAKA
                     ASFIVPAMAVFYVVGGISIIVINSDALMPAVNLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            15758..17014
                     /locus_tag="WGO65_000450"
     CDS             15758..17014
                     /locus_tag="WGO65_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ0326790.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTASRYQ
                     INKERMVRMSEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFGNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQALFWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            17195..18004
                     /gene="zupT"
                     /locus_tag="WGO65_000451"
     CDS             17195..18004
                     /gene="zupT"
                     /locus_tag="WGO65_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ0326791.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            18101..20938
                     /locus_tag="WGO65_000452"
     CDS             18101..20938
                     /locus_tag="WGO65_000452"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ0326792.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLANNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPGFALPEKYANLDRFVARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGTVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPGMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEVALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESEDAPIAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNTKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
BASE COUNT         5063 a         6341 c         5388 g         4197 t
ORIGIN      
        1 acgatataag tatttttatt ttaatccgcc atattaacgc actcggccaa acagcataaa
       61 ggcacgggca gcccgatttg gattgaccta tttccccgac accctcgcca tcagctcgtc
      121 cgccaaggca agatacgcct tggtaccctt tgcctgcgcg tcgtaagcca taaccggcat
      181 accgtggctc ggggcttccg caaggcggat attgcgcggg atgacggttt caaaaagcaa
      241 atccccgaaa tggctgcgca actgttcgct gacttcggca accagcctgc tgcggctgtc
      301 gtacatcgtg cgcacgatgc ccgtgatgtc caaatcggga ttgaccgcct gacggatttt
      361 gcgcacggtc gcaatcaaat cggaaatccc ctcaagcgcg taatattcgc acaacatcgg
      421 cacaatcacg ccgcctgccg ccaccaagcc gttaagcgtc aacagcgtca gcgaaggcgg
      481 gcagtcgatc aggataaagt cgtaatcttc cgccactgcc ttgagcgcgt ttttcaaacg
      541 cacttcccgg gcgatttcct gtaccaactc gatttccgca ccggccagcg cgcggttcgc
      601 gcccaacaca gcgtatccgc cctctttgct gcgtaccgcc gccgactgca cgtccgcatc
      661 gcccaataag acctgataaa cgccggactg caaactcgcc ttgtcgatgc cgctgcccgt
      721 cgtcgcattg ccctgcggat ccaaatcgac caccagcacg cgtttgccgc gcgatgccag
      781 cgaagccgcc aaatttaccg tcgtcgtcgt tttgcccaca ccgcccttct gattggcgat
      841 ggcaaggatg ttcacactca tgtcccgccc gtgtcccgtc tgatgttgaa aaaagaaaaa
      901 cagtgcggaa ttttaccttt atccgcacaa aaaaggtatt catgccgtct gaagcgcgct
      961 caacgcttgc ggagtatggc gatatggcgt tccgcatcca agcccggcac gtcaagcctt
     1021 tggacttttt cgacgcacac atcctgcggc aggcggtcga tttccccctg cggatacacg
     1081 cccttcatcg ccgcccagta tccgccgtct ttcaacagat gcaccgtcca cgacacaaaa
     1141 tccgccagtt ctgcaaacgc acggctggta accacgtcgg cacgcacgtc cgaaaccgcc
     1201 tccacgcgtc cggataccac gcgcacattg tccaatccca actcgataac cgcctgctgt
     1261 aaaaaagccg ttttcttcgt attcgcatcc aaaagggtta tctgcacatc cggacggcac
     1321 accgccgccg gaatgccggg ctgaccgccg cccgaaccga catccagcat cgtttgcaca
     1381 ccctcgatat ggggcagcag cgtcaggctg tccaaaagat gatggacaat cattttttcc
     1441 tcgtcgcgca gggcggtcag attgtaggtt ttgttccact ttttcaacaa atccacatag
     1501 gccaaaagcc tgtcctgcgc cgtttccgaa atatccagcc ccatcgcggc aatgcctgca
     1561 cgcaggcgtt ctttgcgttc catatctgct tcctgtccat attcaggcgt aatgttaacg
     1621 gaaaaacatg ccgtctgaaa ccgcgtgtcg ggtttcagac ggcatccccc gttccgtcct
     1681 attttttcct gtgaaacacc accggctgca ccggcgcgcg cgtcaggtgt ttgggcagcg
     1741 tcagcatcgc caccagccac acggcaaacg catattccga caccgcctcc ccgcgcagcc
     1801 cgcccaaggc ggcattgccc aacaacagct cgaaaatcca agccaccgct gctgccgcgc
     1861 aagccgccac gcccgacaga aacagcgcgt aacgcgaatg ccgccgcatc atctttttgt
     1921 acgccagctt cgtcaggcct gccgacacga ccaggttcaa aaacgcaaac acaccgaaca
     1981 caaaccaaac agtaccgtcc caaccgccac gccggccgac accgacacca gcagccctgc
     2041 ccgtccgaac gcaagcgcaa aggcatcatg caccgccaac gcaaacggca acgtcaaaaa
     2101 caccagcccc gcaaacagag ccgcataagg atctttctcg ttcacaaaca tcgccgtccc
     2161 cctcttccga agcagaccgc attataccgc gcactgcctt gccgcccgcc agaaaagttg
     2221 cacaaacaac cgttcatata tatcataacg aaaaaacgcc ggtgtagctc agtcggtaga
     2281 gcagcgcatt cgtaacgcga aggtcggggg ttcgattccc ttctccggca ccaataccaa
     2341 gcacagaccc tcccttcctc gggaagcctg tgctttttca catttccgct tcagacggca
     2401 accgatatga acacctcgca acgcaaccgc ctcgtcagcc gctggctcaa ctcctacgaa
     2461 cgctaccgcc accgccgcct catacacgcc gtgcggctcg gcggggccgt cctgttcgcc
     2521 accgcactcg cccggctgct ccacctccaa cacggcgaat ggataggcat gaccgtcttc
     2581 gtcgtcctcg gcatgctcca gtttcaaggg gcgatttact ccaaggcggt ggaacgtatg
     2641 ctcggcacgg tcatcgggct gggcgcgggt ttgggcgttt tatggctgaa ccagcattat
     2701 ttccacggca acctcctctt ctacctcacc gtcggcacgg caagcgcact ggccggctgg
     2761 gcggcggtcg gcaaaaacgg ctacgtccct atgctggcgg ggctgacgat gtgcatgctc
     2821 atcggcgaca acggcagcga atggttcgac agcggcctga tgcgcgccat gaacgtcctc
     2881 atcggcgcgg ccatcgccat tgccgccgcc aaactgctgc cgctgaaatc cacactgatg
     2941 tggcgtttca tgcttgccga caacctgacc gactgcagca aaatgattgc cgaaatcagc
     3001 aacggcaggc gtatgacgcg cgaacgtttg gagcagaata tggttaaaat gcgccaaatc
     3061 aacgcacgca tggtcaaaag ccgcagccac ctcgccgcca catcgggcga aagccgcatc
     3121 agccccgcca tgatggaagc catgcagcac gcccaccgta aaattgtcaa caccaccgag
     3181 ctgctcctga ccactgccgc caagctgcaa tctcccaaac tcaacggcag cgaaatccgg
     3241 ctgctcgacc gccacttcac actgctccaa accgacctgc aacaaaccgt cgcccttatc
     3301 aacggcagac acgcccgccg catccgcatc gacaccgcca tcaaccccga actggaagcc
     3361 ctcgccgaac acctccacta ccaatggcag ggcttcctct ggctcagcac caatatgcgt
     3421 caggaaattt ccgccctcgt catcctgctg caacgcaccc gccgcaaatg gctggatgcc
     3481 cacgaacgcc aacacctgcg ccaaagcctg cttgaaacac gggaacacgg ctgacggtcg
     3541 gacacgatgc cgtctgaaac gcctgccgct tcagacggca tctccaatcc tcctgccttg
     3601 tcgtcatgcc cgaataacgg tactattcgc agttaacagt tttcatgccg tctgaaaggt
     3661 ttgaagcgcg tttcagacgg ctgcccgccc accttatccc accgaaagaa caatatgatt
     3721 aacgatattc aaaaaacagc cgaaggcaaa atgcagcgtt cggtcgaagt actgaaagaa
     3781 aatctggcga aagtgcgtac cggccgcgcg cataccggtc tgctcgacca agtggaagtc
     3841 gaatactggg gcagcatggt tcccgtcagc caagttgcca acgtaacgct tctggacgcg
     3901 cgcaccatcg gcgtgaaacc gtttgagggc aatatggcgg ccaaagtcga gaaagccatc
     3961 cgcgattcaa acttgggact gaacccggca gctgtcggcg atctgatccg cgtgccgatg
     4021 cccatgctga ccgaggaacg ccgcaaagac ctgattaaag tcgtacgcgg cgaagcggaa
     4081 gaaggccgcg tctctatccg caacgtgcgc cgcgatgcca acgaccacat caaaaaactc
     4141 ctcaaagaca aagaaatttc cgaagacgag gcacgtcgcg gcgaagaagc ggttcaaaaa
     4201 ctgaccgaca aatacattac cgaagccgac aaactcctga ctgccaaaga agaagatttg
     4261 atggcaattt aacctgcacg gttcggcgtt cagacggcat ttgaatgccg aaccgcgaaa
     4321 ggcagacatg aaaagcagca cgcaggccgt tttggaacac accgccattc ccaagcatat
     4381 cgccgtgatt atggacggca acggccgttg ggcgaaaaaa cgtttcctcc cgcgcataat
     4441 gggacacaaa cgcggtttgg acgcattgga aaatatggtg aagcattgcg ccaaactggg
     4501 tgtgcaatat ctgaccgtgt ttgccttttc aaccgaaaac tggcgccgcc ccgaagacga
     4561 agtttcgttc ctgatggggc tgtttttaca ggctttgcaa aaacaggtac gccgcctgca
     4621 cgaaaacaat atgcgcctga aaatactggg cagccgcgaa cgcttcagcc gtcaaatcct
     4681 gaagggcatc gaagaggcgg aggcgttgac ggcaaacaat accggtctga ccctgagcat
     4741 tgccgccgat tacggcggca gatgggacat tttgcaggcg gcaaacaaac tgattgccaa
     4801 aggcgtatcc gagattacgg aagacacgct ggcgaaacac ttgatgctgg gcgatgcgcc
     4861 cgagccggat ttgttcatcc gcaccggcgg cgaaacgcgc atcagcaatt tcctactctg
     4921 gcaaatggct tatgccgaac tgtatttcac cgatattttg tggcccgatt ttgacggcaa
     4981 ggctttggac gatgccgtcg cttcgttcca aaaacgcgaa cggcggttcg gacgcacctc
     5041 cgagcaactg cctatcgaac agcaaaggaa ctgaatatgc tgaaacaacg ggtaataacc
     5101 gccatgtggc tgctgccgct gatgctgggc atgctgtttt acgcgccgca atggttgtgg
     5161 gctgcatttt gcggactgat tgccctgatt gccttgtggg aatatgcccg tatgggcggt
     5221 ttgtgcaaaa ttaaaaccaa ccattacctc gccgcaacct tggttttcgg cgtggttgcc
     5281 tatgcgggcg gctggatgct gcctaatttg gtttggtatg ttgttttggc attttggctc
     5341 gccgttatgc ctttatggtt gagattcaaa tggaggctca acggcggttg gcaggtttat
     5401 gccgtcggct ggcttctggt catgccgttt tggttcgcgc tcgtatccct gcgcccgcat
     5461 cccgatgatg ccctgccgct gctcgccgtg atgggtttgg tgtgggttgc cgatatttgc
     5521 gcgtatttca gcggcaaggc gttcggcaaa cacaaaatcg caccggcaat cagccccggc
     5581 aaaagctggg aaggtgcaat cggcggcgcg gtttgcgtgg cagtgtacat gaccgccgta
     5641 cgaagtgccg gctggctggc attcgataca ggctggttcg ataccgtgtt aatcggtttg
     5701 gtgctgaccg ttgtcagcgt atgcggcgac ctgttggaaa gctggctcaa gcgcgcggcg
     5761 ggcattaaag acagcagcaa cctgctgtcc ggacacggcg gcgtgttcga ccgtaccgac
     5821 agcctgattg ccgttatcag cgtctatgcg gcgatgatgt cggttttaaa ttgattctat
     5881 gccgtctgaa aacgcttcag acggcatccg gtataaagtt atccccatta tgacaccaca
     5941 agtcctgacc atattaggca gtaccggcag cataggcgaa agcacgctgg acgttgtctc
     6001 cctccacccc gaaaaattcc gcgtattcgc gctggcaggg cataagcagg tcgagaaact
     6061 ggcggtgcaa tgccggacgt tccgccccga atatgccgtc gttgccgatg ccgaacacgc
     6121 cgcccggctt gaagctttgt tgaaaggcga cggcacggcg acgcaggttt tacacggcgc
     6181 gcaggcattg gttgacgttg cctctgccga cgaagtcagc ggtgtcatgt gcgccatcgt
     6241 cggggcggtg gggctgcctt ccgcgctcgc agcggcgcaa aaaggcaaaa ccatttatct
     6301 ggcgaacaaa gagacgctgg tggtttccgg cgcgttgttt atggaaaccg cccgtgcaaa
     6361 cggcgcggca gtgctgcccg tcgacagcga acacaacgcc gttttccaag ttttgccgcg
     6421 cgattacgcc ggccgtctga acgaacacgg catcgcttcg attatcctga ccgcttccgg
     6481 cggcccgttt ctgaccgccg atttaaacac gttcgacagc attacgcccg accaagcggt
     6541 caaacacccc aattggcgta tgggacgcaa aatctccgtc gattccgcca ccatgatgaa
     6601 caaaggtttg gagctgattg aagcgcattg gctgttcaac tgtccgcccg acaaactcga
     6661 agtcgtcatc catccgcaat ccgtgataca cagcatggtg cgctaccgcg acggctccgt
     6721 gttggcgcaa ctgggcaatc ccgatatgcg aacgcccatc gcttattgtt tgggtttgcc
     6781 cgagcgcatc gattcgggtg tcggcgacct ggatttcgac gcattgtccg cgctgacctt
     6841 ccaaaagccc gactttgacc gcttcccctg cctgaagctc gcctatgaag ccatgaacgc
     6901 aggcggagcc gcgccctgcg tattgaacgc cgccaacgaa gccgccgtcg ccgccttttt
     6961 ggacgggcag attaagttta ccgacattgc caaaaccgtc gcccattgtc tcgcacaaga
     7021 cttttcagac ggcataggcg acataggggg gctcttggcg caagatgccc ggacacgcgc
     7081 acaagcgcgg gcatttatcg gcacactgcg ctgatgccgt ccgaacaccg ttatcaaagg
     7141 aaaaccattt gcacaccctt ctagctttta tcttcgccat cctgattttg gtcagcctgc
     7201 acgagttcgg acactacatc gttgccagat tgtgcggcgt caaagtcgta cgcttttccg
     7261 tcggcttcgg caaaccgttt ttcacccgaa agcgcggcga caccgaatgg tgcctcgccc
     7321 cgattccgtt gggcggttac gtcaaaatgg tcgatacgcg cgaaggcgaa gtatcagaag
     7381 ccgatttacc ctacgctttt gacaaacaac accccgccaa gcgcatcgcc atcgtcgccg
     7441 ccggcccact gaccaacctc gcactggcgg ttttgctgta cggactgagc ttttccttcg
     7501 gcgtaaccga actgcgcccc tacgtcggca cagtcgaacc cgacaccatt gccgcccgcg
     7561 ccggcttcca aagcggcgac aaaatacaat ccgtcaacgg cacacccgtt gcagattggg
     7621 gcagcgcgca aaccgaaatc gtcctcaacc tcgaagccgg caaagtcgcc gtcggcgttc
     7681 agacggcatc gggcgcgcaa accgtccgca ccatcgatgc cgcaggcacg ccggaagccg
     7741 gtaaaatcgc aaaaaaccaa ggctacatcg gactgatgcc ctttaaaatc acaaccgttg
     7801 ccggcggcgt ggaaaaaggc agccccgccg aaaaagcagg cctgaaaccg ggcgacaggc
     7861 tgactgccgc cgacggcaaa cccatcgcct catggcaaga atgggcaaac ctgacccgcc
     7921 aaagccccgg caaaaaaatc accctgaact acgaacgcgc cggacaaacc cataccgccg
     7981 acatccgccc cgatactgtc gaacagtccg accacaccct gatcgggcgc gtcggcctcc
     8041 gtccgcagcc ggacagggcg tgggacgcgc aaatccgccg cagctaccgt ccgtctgtta
     8101 tccgcgcatt cggcatgggc tgggaaaaaa ccgtttccca ctcgtggaca accctcaaat
     8161 ttttcggcaa actaatcagc ggcaacgcct ccgtcagcca tatttccggg ccgctgacca
     8221 ttgccgacat tgccggacag tccgccgaac tcggcttgca aagttatttg gaatttttgg
     8281 cactggtcag catcagcctc ggcgtgctga acctactgcc cgtccccgtt ttggacggcg
     8341 gccacctcgt gttttatact gccgaatgga tacgcggcaa acctttgggc gaacgcgtcc
     8401 aaaacatcgg tttgcgcttc gggcttgccc tcatgatgct gatgatggcg gtcgccttct
     8461 tcaacgacgt tacccggctg ctcggttaga ttttacgttt cggaatgccg tctgaaaccg
     8521 cattccgcac cacaaggaac tgacaatgaa actgaaacag attgcttccg cactgatggt
     8581 cttgggcata tcgcctttgg cacttgccga cttcaccatc caagacatcc gcgtcgaagg
     8641 cttgcagcgt accgagccga gtaccgtatt caactacctg cccgtcaaag tcggcgacac
     8701 ctacaacgac acacacggca gtgccatcat caaaagcctg tacgccaccg gtttctttga
     8761 cgacgtacgc gtcgagactg cggacgggca gctcctgctg accgtcatcg aacgccccac
     8821 catcggctcg ctcaacatca ccggcgcaaa aatgctgcaa aacgacgcca ttaagaaaaa
     8881 tctcgaatcg ttcgggctgg cgcagtcgca atactttaat caggcgacac tcaatcaggc
     8941 agtcgccggc ctgaaagaag aatacctcgg gcgcggcaaa ctcaatatcc aaatcacgcc
     9001 caaagtaacc aaactcgccc gcaaccgcgt cgacatcgac atcacgattg acgagggcaa
     9061 atccgccaaa atcaccgaca tcgaatttga aggcaaccaa gtctattccg accgcaaact
     9121 gatgcggcag atgtcgctga ccgaaggcgg catttggaca tggctgacac gaagcaacca
     9181 attcaacgag cagaaatttg cccaagacat ggaaaaagta accgacttct accaaaacaa
     9241 cggctacttc gatttccgca tcctcgatac cgacatccaa accaacgaag acaaaaccaa
     9301 gcagaccatc aaaatcaccg tccacgaagg cgaacgtttc cgttggggca aagtctccat
     9361 cgaaggcgac accaacgaag tccccaaagc cgaactggaa aaactgctga ccatgaagcc
     9421 cggcaaatgg tacgaacgcc agcagatgac cgccgttttg ggtgagattc agaaccgcat
     9481 gggctcggca ggctacgcat acagcgaaat cagcgtacag ccgctgccaa acgccgaaac
     9541 caaaaccgtc gatttcgtcc tgcacatcga accgggccgg aaaatctacg tcaacgaaat
     9601 ccacatcacc ggcaacaaca aaacccgcga cgaagtcgtg cgccgcgaat tgcgccaaat
     9661 ggaatccgcg ccttacgaca cctccaagct gcaacgctcc aaagagcgcg tcgagctttt
     9721 gggctacttc gacaacgtac agtttgatgc cgtcccgctt gccggcacac ccgacaaagt
     9781 cgatttgaac atgagcctga ccgaacgttc caccggctcg ctcgacttga gcgcgggctg
     9841 ggtacaggat accggcctgg tcatgtccgc aggcgtttcc caagacaacc tgttcggtac
     9901 gggcaagtcg gccgccctgc gcgcctcacg aagcaaaacc acgctcaacg gctcgctgtc
     9961 gtttaccgac ccgtacttca cggcagacgg ggtcagcctg ggctacgatg tttacggaaa
    10021 agccttcgac ccgcgcaaag catcgaccag catcaaacaa tataaaacca ccacggcagg
    10081 cgcaggcatc cgcatgagcg tgcctgttac cgaatacgac cgcgtgaatt tcggtttggt
    10141 ggcagaacac ctgaccgtca acacctacaa caaagcgccc aaacactatg ccgactttat
    10201 caagaaatac ggcaaaaccg acggcacaga cggcagcttc aaaggctggc tgtacaaagg
    10261 taccgtcggc tgggggcgca acaaaaccga cagcgcgtta tggccgacgc gcggctacct
    10321 gacgggcgtg aacgccgaaa tcgccctgcc cggcagcaaa ctgcaatact actccgccac
    10381 ccacaaccaa acctggttct tccccctgag caaaaccttc acactgatgc ttggcggcga
    10441 aatcggctat gccaacggct acggcaaaac caaagaaatc cccttctttg aaaacttcta
    10501 cggcggcggc ctgggttcgg tacgaggtta cgaaagcggc acactcggtc cgaaagtcta
    10561 tgacgaatac ggcgaaaaaa tcagctacgg cggcaacaaa aaagccaacg tctccgccga
    10621 gctgctcttc ccgatgcccg gcgcgaaaga cgcgcgcacc gtccgcctga gcctgtttgc
    10681 cgacgcaggc agcgtgtggg acggcaaaac ctacgacgac aacagcagtt ccgcgaccgg
    10741 cggcagggtt caaaacattt acggcgccgg caatacccat aaatccacct ttaccaacga
    10801 attgcgctat tccgccggcg gcgcggttac ctggctctcg cctttaggcc cgatgaaatt
    10861 cagctacgcc tacccgctga agaaaaaacc ggaagacgaa atccaacgct tccaattcca
    10921 actcggcacg acgttctaat cccgcaaatg ccgtctgaag cccttcagac ggcatttcgc
    10981 ggcaacattc gaaggagttt taccatgacc cgtttgaccc gcgcgtttgc cgcggctctg
    11041 atcggtttgt gctgcaccgc aggcgcgcac gccgacacct tccaaaaaat cggctttatc
    11101 aacaccgagc gcatctacct cgaatccaag caggcgcgca agattcaaaa aacgctggac
    11161 agcgaatttt ccgcccgtca ggacgaattg caaaaactgc aacgcgaagg tctggatttg
    11221 gaaaggcagc ttgccgaagg caaactcaga aacgcaaaaa aggcgcaagc cgaagaaaaa
    11281 tggcgcgggc tggtcgcaac gttccgcaaa aaacaggcgc agtttgaaga agactacaac
    11341 ctccgccgca acgaagagtt tgcctccctc cagcaaaacg ccaaccgtgt catcgtcaaa
    11401 atcgccaaac aggaaggtta cgatgtcatt ttgcagaacg tgatttacgt caacacccaa
    11461 tacgacgtta ccgacagcgt cattaaagaa atgaacgccc gctgaccctt tcagacggca
    11521 taccgaacag gaaaaccatg attccggcca cctacaccct gtcccaaatc accgcgcggc
    11581 tcggcggcga atggcgcggc gaggacattt ccgttaccgc cgtgcgcccg ctcgcagacg
    11641 cgcaggcgga acacatcagc ttccttgcca atccgaaata caaagccgaa gtccacgaca
    11701 gcagcgcggg cgcggtcatc gtttccgcca aagcggcaga cggatttgaa gggcgcaacc
    11761 tgattgtcgc cgacgacccc tatctctatt tcgccaaagt cgcccgcctg ttttcacccg
    11821 tcgtcaaagc gcgcggcggc atccatccga ccgccgtcgt cgaaccgggc gcgaccgttc
    11881 ccgccagctg cgaaatcggc gcgaacgtct acatcggcgc aaacaccgtg ctcggcgaag
    11941 gctgccgcat cttggcaaac gccgtcgtcc aacacgattg caaactgggc gacgaagtcg
    12001 tcctgcatcc caacgccgtc gtttattacg gctgcacact gggcagacgc gtcgaaatcc
    12061 acagcggcgc ggtcatcggc gcggacggtt tcggactcgc ttttgccggc gattcatggt
    12121 ttaaaatccc gcaaaccggc gcggtaacgc tgggcgacga cgtagaaatc ggctcgaaca
    12181 ccaacatcga ccgcggcgcg atgagcgaca ccaccgtcgg caacggcacc aaaatcgaca
    12241 accaagtcca aatcggacac aactgcaaaa tcggttcgca caccgtcatc gccgccaaaa
    12301 ccggcatctc aggcagcgta accataggca gctactgcat catcggcggc ggcgtcggta
    12361 cggtcggaca catcgaaatc gccgacaaaa ccaccatcgg cggcggcacg tccgtcaccc
    12421 acagcattac cgaaagcggc aaacacctcg ccggcatctt cccgatgtcc acccataaag
    12481 aatgggcgcg caacgctgtt tacatccacc gcttaagcga aatgaacaaa cggctcaaaa
    12541 cactggagca gcagctttca gatgccggtc aagacagcaa ataaccaaac cgactttatt
    12601 caaggaatac gacagacatg gacgtacaac tccccatcga agccaaagac atccaaaaac
    12661 tcatccccca ccgctatccg tttctccagc tcgaccgcat caccgccttc gagccgatga
    12721 aaaccctgac cgcgattaaa aacgtcacca taaacgaacc ccagttccaa ggccatttcc
    12781 ccgacctgcc cgtgatgccc ggcgtactca tcatcgaagc gatggcgcag gcgtgcggca
    12841 cgttggcgat tttgagcgaa ggcgggcgca aagaaaacga attcttcttc ttcgccggca
    12901 tagacgaagc ccgtttcaaa cgccaagtca tccccggcga ccaactcgtc tttgaagtcg
    12961 agctgctgac cagccggcgc ggcatcggca aattcaacgc cgttgccaaa gtagacgggc
    13021 aagtcgccgt cgaagccgtg attatgtgcg ccaaacgcgt ggtttgagtg ttcagaaaaa
    13081 ggtcgtctga aagttttcag acgacctgtt gccgtcgcgc atcttcgcgg caacacgaca
    13141 ggaaaggaaa aacatgaccc tcatccaccc gaccgccgtc atcgacccca aagccgaact
    13201 cgactccagc gtcaaagtcg gcgcgtacac cgtcatcggc cccaacgtcc aaatcggcgc
    13261 gaataccgaa atcggcccgc acgccgtcat caacggccac accagcatcg gcgaaaacaa
    13321 ccgcattttc caatttgcca gcctcggcga aatcccgcag gacaaaaaat accgcgacga
    13381 gccgaccaag ctgattatcg gcaacggcaa caccatccgc gaattcacca cctttaattt
    13441 aggcacggta accggcatcg gcgaaacccg tatcggcgac gacaactgga ttatggcgta
    13501 ctgccacctc gcgcacgact gcgtggtcgg caaccacacc atcttcgcca acaacgcctc
    13561 gcttgccgga cacgttacca tcggcgacta cgtcgttttg ggcggctaca cgctggtttt
    13621 ccaattctgc cgcatcggcg actacgccat gaccgcgttt gccgcaggcg tacacaaaga
    13681 cgtgccgccc tactttatgg catcgggcta ccgcgccgaa ccggcggggc tcaacagcga
    13741 aggcatgcgc cgcaacggtt ttaccgcaga gcagatttcc gccgtcaaag acgtgtacaa
    13801 aaccctctac catcgcggca ttccgtttga agaagccaag gcagacattc tccgccacgc
    13861 cgaaacccaa gccgagcttg ccgtattccg agactttttc gcacaatcgg cacgcggcat
    13921 catccgctga ccgtaccctt ttgatgccgt ctgaaacccc gacagcgttt cagacggcat
    13981 cggttttttt aaagcggtat attgtttgat cgatgcatca ttttgtagat ttcagaagta
    14041 tggcagtcgg cagcattgat gatttctgcc gcccgccaca tccctattcg gacatttcat
    14101 tgtgacctcc gccgctgctt ttaaagaatt gcaaacaatt tccgcagctt gaaccggcct
    14161 gttttcggcg aaaaaaacca aaagtatttt tcaaaagaac atattgtctt attggatatt
    14221 ctgtgctgaa attactggac actacaaaag tggtgtttta aaaataaaac agacaaagga
    14281 gaaagccatg caagtgtttt tagacaatcc caaagccttt tttgaaaccg tcagcggctg
    14341 ggtttgggga cctttgatgc tgatgctgct ggtcggcacg ggtattttgc tgactgtttt
    14401 attaaaaggt ttgcagttca cgatgttggg ttatgcgctg aaacaggcgt ttatgccgcc
    14461 aaagaagcat aaaagcggcg aaggccacga aggcgatatt tcccatttcg cggcgttgat
    14521 gaccgcgctg tccgccacca tcggtacggg taacatcgcc ggcgtggcga ctgcggtggt
    14581 aaccggcggc ccgggcgcgg tattttggat gtggatgacc gccattttcg gcatggccac
    14641 caaatacggc gaaggcgtgt tggcggtgaa ataccgcgtc aacaattcca aaggcgaaat
    14701 gtccggcggc ccgatgtatt acatcgaaaa aggcttgggc aaaaactgga aatggatggc
    14761 gttggcgttc gctttattcg gcacattcgc ttccttcggt atcggcagct cggtgcagtc
    14821 caactcggtt gcacaggcgg tgcaaaccag cttcggtatc gaacctgcct ataccggcat
    14881 tacgttgacc gttctgactg ccatcgttgt gttgggcggt atcaaaggca tcgccaaagc
    14941 cgcttctttc atcgtacctg ccatggcagt gttttatgtg gtaggcggta tctccattat
    15001 cgtgattaat tctgatgcgc tgatgcctgc cgtcaacctg attttctccg atgcgttcag
    15061 cgcgcaggcc gtggcaggtg gtgctatcgg taccgttatc cgctacggcg tggcgcgcgg
    15121 cgtgttctcc aatgaggcgg gcatgggttc tgcgccgatt gccgccgcag ccgccaaaac
    15181 cgaccatccc gtccgtcagg ccttggtttc tatgaccggt acgtttttgg ataccatcgt
    15241 cgtttgctcg attaccggca tcgtgttggt aatgggactg ctcggcgcgg gcggcgagtt
    15301 tgtcaaacct gaagtgagcg gcgcggcact gacaaccgtc acattccaaa aaatgctgcc
    15361 cggcatcggc ggctggattg tcaccatcgg cctgattttc tttgcctact caaccattct
    15421 cggctggtgc tattacggcg aaaaatgcgc ggtgtatgtt ttcggcgaga aatttgccgg
    15481 tttgtaccgc gtcggttatg tttcttccgt tatgctgggt accgtgttga gccttgattt
    15541 ggtgtggctg gcttcggaca cattcaacgg cttgatggca ttgcccaacc tgattgcact
    15601 tttattgatg gcgaaagtca tcgtcaatga aacgcgcgac ttcaaacaaa aaatcacaaa
    15661 cggcgaattg ccgcattaat atccgaggcc gtctgaatcc gaatattcag acggcacggc
    15721 atattaagtc tgttttgaaa aagccggagg gagaaacatg aaagtgcttg ttttaggtgc
    15781 gggtgttgcc ggcgtgtctt ccgcgtggta tctggcagag gccggacatg aagtaacggt
    15841 catcgaccgc gccgagggtg tggcgatgga aaccagtttt gccaacgcag gccagctttc
    15901 ttacggctat accacgcctt gggctgcacc cggtattccg accaaagcgc tgaaatggct
    15961 gtttaaaagc catccgcctt tgctgtttcg ccccgacggc agcctgtatc aaatcgaatg
    16021 gctgtggcgg atgctgcaaa actgcacggc atcccgctat caaatcaata aagagcgcat
    16081 ggtcaggatg tccgaataca gccgtgaaat gttccgccgt tttgaagcgc aaaccggcat
    16141 gaattttgag ggacgcaaaa aagggacgtt gcagattttc cgtcaggcta aagaagttga
    16201 agcggcagaa caagacattg ccgttttgga acgctacggc gtgccgtacc gccgtctgaa
    16261 gcccgaagaa tgcgcagaat tcgagcctgc gctggcacgc gttaccgcca aaattgccgg
    16321 cggcctgcac ctgcctgcgg atgcaaccgg cgactgccgc ctcttcactg aaaacctata
    16381 caaattgtgt caggaaaagg gcgtacggtt tcatttcaac caaaccatca gccgcatcga
    16441 ccacaacggg ctgcgcatca aaaccgttga aaccgaaaca gggcggtttg aagcagatgc
    16501 cgttgtctgc gcgctcggtt gtttcagcag gacggttttg gcgcagttgg atctcaatct
    16561 gcccatttat cccgtcaaag gctattcctt gaccctgccg gtcacaaatt ccgacggcgc
    16621 accggtgtcc accgttttag atgaaagcta caaagtcgcc atcacgcgct ttggcaacag
    16681 aatccgcgtc ggcggtatgg cggaattgtc gggctacgaa atcaaactgc ccgaaaaacg
    16741 ccgcgaaacc ttggctttgg tcgtcaacga tttgttcccg gaaggcggcg atttgagcca
    16801 agcattgttc tggagcggcc tcagaccgat gacgcccgac agcacgccgt taatcggccg
    16861 cacccgcttt gaaaacttat tcttgaatac aggccacggc actttgggct ggaccatgtc
    16921 gctgggttcg gcaaaattaa ccgccgatat cgtgagcggc aaagacaccg aaatccgaag
    16981 cgacgatttg agcctgtcgc gctatcaggc ttaagctgga aagcgataag gtttagcgac
    17041 atttcaaatt taaaggccgt ctgaaaatca tttttcagac ggccttttgt ttcaaaattc
    17101 gggtatccaa tcaataaaat aaataataac tattatcata cgcattaaaa agtgttagtc
    17161 tgaacctgtc ccagctttta gcgcaggttt tcccatgccc gatttttcga tgtccaattt
    17221 ggccgttgcc ttttccatta cgttggctgc cggtttgttt accgtattag gcagcggctt
    17281 ggtgatgttt tccaaaacgc ccaatccgcg cgtgttgtcg tttggtttgg catttgccgg
    17341 cggtgcgatg gtgtatgttt ccctgacgga gattttcagt aagtccagcg aggcgttcgc
    17401 tgaaatttat gataaagacc acgcgtttgc ggcggcgacc atggcatttt tggccgggat
    17461 gggcggcatt gcgctgattg accgtctggt gccgaacccg catgaaactt tagacgcgca
    17521 agacccgtcg tttcaagaaa gcaaacgccg ccatatcgcg cgagtcggca tgatggcggc
    17581 gtttgcgatt actgcgcaca atttccccga aggcttggcg acgttttttg ccacattgga
    17641 aaatccagca gtcgggatgc ctttggcctt ggcgattgcc atccataata ttccggaggg
    17701 catttccatc gccgcgccgg tttattttgc cacccgcagc cgtaagaaaa cggtgtgggc
    17761 gtgtctgcta tccggcttgg ccgagccgtt gggggcggct ttgggctatt tggttttgca
    17821 gccgtttttg tcgcctgccg tgtttggttc ggtattcggc gtgatagccg gtgtgatggt
    17881 gtttttggcg ttggacgagc tgctgccggc tgccaaacgc tattcagacg gccatgaaac
    17941 cgtttacggc ctgacaatgg gcatggcggt gattgccgtc agcctggtat tgttccattt
    18001 ttaaaacctg tatgccaaag gccgtctgaa atgattcaga cggccttttt gcgttataat
    18061 ccgcacatca aaaataacct taatttcaca aagttccatc atgttagaca aatacaatcc
    18121 agccgaaatc gaatccaaac attatcaaaa ctgggaagag cagggctatt tccaacctga
    18181 tatggatttg accaaaccgt ctttctccat ccaactgccg ccgcccaacg taaccggcac
    18241 gctgcacatg ggccatgcct tcaaccaaac cattatggac ggcctgaccc gctactaccg
    18301 catgaaaggc tgcaacaccg cctggattcc cggcaccgac cacgcgggca tcgccacgca
    18361 aatcgtggtc gagcgtcagc ttgccgcgca aaacgtgtcc cgtcatgact tgggccgcga
    18421 aaaattcttg gaaaaagtgt gggaatggaa agaagtgtcc ggcggcacga ttacccaaca
    18481 aatgcgccgc gtgggctgct ctgccgactg gacgcgcgag tatttcacga tggacgacgt
    18541 gcgcgccgaa accgtgacag aagtgttcgt acgcctgtat gaacagggct tgatttatcg
    18601 cggcaaacgc ttggtaaact gggatcccgt gcttggcacg gcggtatcgg atttggaagt
    18661 ggaaagcgtg gaagaacaag gctctatgtg gcacatccgc tatccgctgg ccaacaatcc
    18721 tgccgaagcc gttatcgtgg cgaccacccg ccctgaaacg ctgctgggcg acgtggccgt
    18781 tgccgtcaat cctgaagacg aacgttatac ccatttgatt ggcaaggaat taatcctgcc
    18841 gctgaccggc cgcaccatcc ccgtgattgc cgatgaatac gtcgaaaaag acttcggtac
    18901 cggctgcgtg aaaatcacgc ctgcgcacga cttcaacgac tacgaagtcg gcaaacgcca
    18961 cgacacgcgc ttggttaatg tgttcgactt ggaagccaaa gtgctggcaa acgccgaagt
    19021 gttcaacttc aaaggcgaag cgcaaccagg ctttgccctg cctgaaaaat acgcaaactt
    19081 agaccgcttt gtcgcgcgca aacaaatggt tgccgatttg caggaacaag gcttcttggt
    19141 cgaaatcaaa gcgcatacgc tgatgacgcc gaaaggcgac cgcacaggtt cggtcattga
    19201 gccgatgctg accagccaat ggtttgtcgc catgtccgcc acaccgaacg gcggcgagcc
    19261 tgacagcgaa ttcaaaggct tgagcctcgc cgacaaagcc aaaaaagccg tcgacagcgg
    19321 cacggtacgc tttatccccg aaaactgggt caacacttac aaccaatgga tgaacaacat
    19381 ccaagactgg tgtatctcgc gccaactgtg gtggggacat caaatccctg catggtacga
    19441 caacgaaggc aatgtgtacg ttgcccgcaa tcaggaagaa gccgaaaaac aagccggcaa
    19501 aaccggtttg acccgcgaag aagacgtatt ggacacatgg ttctcctccg cgctcgtgcc
    19561 gttctccacg ctcggctggc cgtctgaaac cgacgaactc aaagccttcc tgccgtccaa
    19621 cgtcttggtt accggctacg aaatcatctt cttctgggtg gcgcgcatga ttatgatgac
    19681 cacccacttc accggcaaag taccgtttaa agacgtgtac atccacggca tcgtgcgcga
    19741 ccacgaaggc aaaaaaatgt ccaaatcaga aggcaacgtc atcgaccctg tcgatttgat
    19801 tgacggcatc gacttggaca agctgctggt aaaacgtacc accggcctgc gcaaacccga
    19861 aaccgcgccg aaagtggaag aagccaccaa aaaactcttt cccgaaggca tccccggtat
    19921 gggcgcggac gcattgcgct tcactatggc gagctacgcc agcctcggcc gcagcgtcaa
    19981 cttcgacttc aaacgcgccg aaggctaccg caacttctgt aacaaaatct ggaacgccac
    20041 caacttcgtc ttgatgaaca ccgaaaacca agactgcggc tacggcgcaa ccgctgccga
    20101 accgcgcggc tattccttcc ccgatatgtg gattgtaggc cgtctgaatc agaccatcga
    20161 gcaggttacg caagcctacg aaacctaccg ctttgatttg gcggcagaaa ccctgtacag
    20221 cttcgtatgg aacgactatt gcgactggta tttggaactt gccaaagtgc agcttcaaac
    20281 cggctgcgcc agccgccaac gcgccacacg ccatacctta ttgcgcgtac tcgaagtcgc
    20341 cctgcgcctg ctgcacccga ttatcccgtt cattaccgaa gaactgtggc aaaccgtcgc
    20401 ccctatgtgc gacgcgaaaa ccgccgacag catcatgctc gcccgcttcc ccgaagccga
    20461 cggcggcgat attgttcaga cggcattcga gcaaatgact gtgttgcagg atttgattgg
    20521 cgcagtccgc aacctgcgcg gcgaaatggg catccagccc aacgtgaaag ccccgctgtt
    20581 tgtcgaaagc gcagacgact tggcggacta cctcaaatac ctgccgatga tgacccgcct
    20641 gaccgaagca cggcaagtcg ccgccctgcc cgaaagcgaa gacgcgccca tcgccgtctg
    20701 caacggtgcg cgcctgatgc tgaaagtcga aatcgacaaa gccgccgaaa ccgcccgctt
    20761 gagcaaagaa gccgagaagc tgcaaaaagc cttggacaaa ctcaacacca aactctccaa
    20821 acccggctac accgaaaaag ccccggcgca tctggtggaa aaagacaaag ccgatttggc
    20881 ggagttggaa gacaaaatgg caaaagttca aaatcagttg gcgaagttga aagactgatt
    20941 tgattgaata aacaaggccg tctgaaaaat gtttttcaga cggcctctt
//
LOCUS       DAWFAN010000018        20692 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWFAN010000018 DAWFAN010000000
VERSION     DAWFAN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.018"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(64..240)
                     /locus_tag="WGO65_000453"
     CDS             complement(64..240)
                     /locus_tag="WGO65_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326793.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(243..1601)
                     /locus_tag="WGO65_000454"
     CDS             complement(243..1601)
                     /locus_tag="WGO65_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ0326794.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLAALQTLAKVWRENVNPF
                     VFGITGSVGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1725..3374)
                     /locus_tag="WGO65_000455"
     CDS             complement(1725..3374)
                     /locus_tag="WGO65_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ0326795.1"
                     /translation="MKQSARIKNMNQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSGGGVFADVKNYKRRSKIWLT
                     ILLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAK
                     KASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTA
                     HATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYP
                     WFTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDAR
                     RFQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTL
                     THGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDF
                     WGNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3397..4875)
                     /locus_tag="WGO65_000456"
     CDS             complement(3397..4875)
                     /locus_tag="WGO65_000456"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ0326796.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDGYGAELIGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFRTPWGEGKCHTRLLGRFNAQNLAACVALLCANGYPLDKVLDVLEKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRLLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4900..6645)
                     /locus_tag="WGO65_000457"
     CDS             complement(4900..6645)
                     /locus_tag="WGO65_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ0326797.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6706..6969)
                     /gene="ftsL"
                     /locus_tag="WGO65_000458"
     CDS             complement(6706..6969)
                     /gene="ftsL"
                     /locus_tag="WGO65_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ0326798.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6966..7952)
                     /gene="rsmH"
                     /locus_tag="WGO65_000459"
     CDS             complement(6966..7952)
                     /gene="rsmH"
                     /locus_tag="WGO65_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ0326799.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(7949..8404)
                     /gene="mraZ"
                     /locus_tag="WGO65_000460"
     CDS             complement(7949..8404)
                     /gene="mraZ"
                     /locus_tag="WGO65_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ0326800.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            8683..9900
                     /locus_tag="WGO65_000461"
     CDS             8683..9900
                     /locus_tag="WGO65_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0326801.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEEIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYL
                     PQSHFLLNLGITELLAQVGKTDSPAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVD
                     WAGFRFGDICHKL"
     gene            10032..10108
                     /locus_tag="WGO65_000462"
     tRNA            10032..10108
                     /locus_tag="WGO65_000462"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10066..10068,aa:Met,seq:cat)
     gene            10116..10191
                     /locus_tag="WGO65_000463"
     tRNA            10116..10191
                     /locus_tag="WGO65_000463"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10149..10151,aa:Ala,seq:ggc)
     gene            complement(10482..11303)
                     /locus_tag="WGO65_000464"
     CDS             complement(10482..11303)
                     /locus_tag="WGO65_000464"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ0326802.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEHLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(11336..11980)
                     /locus_tag="WGO65_000465"
     CDS             complement(11336..11980)
                     /locus_tag="WGO65_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0326803.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEREDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11983..12828)
                     /gene="ftsN"
                     /locus_tag="WGO65_000466"
     CDS             complement(11983..12828)
                     /gene="ftsN"
                     /locus_tag="WGO65_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0326804.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKADEVEEK
                     ADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEKKASKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(12934..14430)
                     /locus_tag="WGO65_000467"
     CDS             complement(12934..14430)
                     /locus_tag="WGO65_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ0326805.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLVQTDCQVPQMPSENTKLLDLVDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAVELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14444..14764)
                     /locus_tag="WGO65_000468"
     CDS             complement(14444..14764)
                     /locus_tag="WGO65_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ0326806.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15247..16773
                     /gene="putP"
                     /locus_tag="WGO65_000469"
     CDS             15247..16773
                     /gene="putP"
                     /locus_tag="WGO65_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ0326807.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            17024..20629
                     /gene="putA"
                     /locus_tag="WGO65_000470"
     CDS             17024..20629
                     /gene="putA"
                     /locus_tag="WGO65_000470"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ0326808.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4839 a         5993 c         5055 g         4805 t
ORIGIN      
        1 tcgggcctgc gcccgtttga ggttggcttg ccgcccggca aggtttcgga cggcttttgc
       61 agattaacgt gcagggactt tctcaatcac gcaaattggg taactccccg gatttttacc
      121 gccgcccaag tctaaacata aatcttcata aagatacgcc tgcgtccgca ttccggcata
      181 aaacgcgccg accagcgcgg caatcagcaa taaggcttta aaaaaacgtc ggcttttcat
      241 tttcacttat cctccaatgc ctcgaccact tcttccatct gcataaagcg cgaacctttc
      301 accaacacgg tggcgcgttc gggcaaatcg tggcgcaaca cttgaatcaa cgggtctttg
      361 gcggcgaacc acaaaccgtc cgcgccaaat ttttccgccg cttcgacgct gttgtcgccg
      421 acaaaataag ccgcttcgat gccttggtcg cgggcatacg cgccgacttc ggcgtgcata
      481 gcggcggctt cgtcctcgcc cagttcgccc atatcgccca ttacgaaaat acgcggcgca
      541 ggcatacgcg ccaacacgtc aatcgcggct ttcatgctgt ccgggttcgc attataagta
      601 tcgtcaatca gggttgcgcc cttgattccg gatttgacgt tcagacggcc tttgatattg
      661 ctgaagcctt tcaaaccttc cgccacatcg ttcaaactca aacctgcagc caaagacagc
      721 gcggcggcag cggcggcgtt gtggacattg tggcggccgg gaacaggcag caccacggcg
      781 gcgcgttcgt cgccgcacac caaatcaaat tcgcacgaca acggtttcag cacgatattt
      841 tccgcgtgga catcgccgct atcgatgccg aaagtgcgcg tattcaaatt aagcgttgcc
      901 gttttgaaga cagccatatt ggcatcttct tgaggaatca gtgccatgcc gtctgaacat
      961 aagccttgat aaatctcgct tttcgctttg gcaatatcgc ccactccgtc gaaaccgcag
     1021 ccgacatggg cgcgcatggc gttgttgacc aatgcggcat cgggtttggc gatttgtgtc
     1081 aaaaccgcca gttcgccaaa atggttcata cccatttcaa tcacggcata gcggtgtttt
     1141 tcgtttaatt tcaacaaagt caacggcaat ccgatgtggt tgttgaagtt gcctgccgtc
     1201 gccaaaacgg cattatcgcc gaaacggcgg cgcaataccg cagccagcat ctccttcacc
     1261 gtcgtcttgc cgaccgaacc ggtaatgccg aacacaaacg gattcacatt ttcacgccac
     1321 acctttgcca acgtttgcaa tgcggcaagc gtgtcatcga ctttcaacgc gccatccatt
     1381 gcaacgcaat cttcgcgcga aaccacaacc gccgccgcac cagcagccaa tacgtcttca
     1441 acaaaatcat gcgcatcaaa ccgcccgccc gccaatgcga aaaacacatc gcccgcgcgg
     1501 atgtcgcggc tgtcggttac gatgcgcgac acgggtttgc tttcagacgg catcggaagc
     1561 ttgagggctt ggcagatgaa atttaggtcc agtggtttca tatttacttt cgttaatatt
     1621 cgggcggcgg acacatcggt agcggctgat tttttatcgc ctgttttact gtggtaaaac
     1681 acagattatt ttcccattct cattcggcat tttttctgta cgtatcattt tttagacgta
     1741 tttttagtcg atttgccttt tcccgcatac cacggcgcgg ggtcgtcggg cagtccgtcg
     1801 ataaaggcaa ggttattgcc ttcgccctgc acatcgggaa cattccccca aaaatcatag
     1861 ccgtcatcgg gcaactcgtc ggtttcgata cccgtccaac tgccgaatcc gcgtaaaaaa
     1921 ttaaacgcgc tgcgcctcac tttaatcatt tcgcgccgcg tgtcatcgga cgaaatttta
     1981 accagcggca cgccgtagct ttgacgcttc cacgcaccat gtgtcaacgt tcgctcgccg
     2041 cctttaccga catgcatcaa accgtggtcg gaaaagtaaa ccaaagacca gctttcttta
     2101 ttttcattca atatcttaac tgtatcttct aaaaatttat cggtttgcgc gatggtggaa
     2161 acatagcagg atattttttc agtttgatac tgaaaccgcc gcgcatcctt atccaaacgt
     2221 gtgcaaaaat cactgtgcga acccatcagg tgcatcacaa tcagccgagg cttcgtgcct
     2281 gcatttccta tcaaaacccg tttgaacgcc ggcaacaaaa ggcggtcgct caaccccgcg
     2341 cttttgccat aatcacccct ttgggtaaac cacggataat cgctgcgtag ggcataggtg
     2401 gaaatttcgt tggcaaaatg ccccaacatt ccttgattag acagccacgc cgtccgaaaa
     2461 cccgcctgct tcgccaagct gacgatgtta ttgttcggtt ctcccggcag ccccaaagtc
     2521 tgcggcagcg aaagattcgt cgcgtgggcg gtcgattggt aaccgtttat caacagccct
     2581 ttggtctgac tcaaaaaagg cgtatcgggc aatgggaaac cgtaaacatt catataatcc
     2641 gaacgcgcgc tctcaccgat aaccacaaca taatttttat acttgggcgc aacatgacgg
     2701 atatgccatg ttgacgcttt ttttgctgct tccaaaatgt gggcgcgctt ggcggcatat
     2761 tgtgccggcg cggaagccaa atcgtaatac aggtcgaaaa tattcaacaa caggccggca
     2821 tcaggttctc gcaaatcttt atcgctggcg attttatcca tcaccgcgca ggacaaaatc
     2881 aaagtcaata atatagtcag ccatattttg ctgcggcgtt tatagttttt tacgtcagca
     2941 aatacccccc cccccgatac acaatatttc caaactgtca agccaaaaat gaaaaataat
     3001 gcctgcacaa aataaagcga cccgggaaga ttgccgacaa attcacgcgc ctcggcagga
     3061 ttgctttcca atatcgaacc gactatctga taagacggcg caccatacaa ccagccgacc
     3121 ggcaaatata gggcggttgt gccgacgtaa atcagtaaaa caactgaaga aacgcgcggg
     3181 aaaccgcgtg ccaataataa taaaaataca accgaagcca aagcacccac ggcagaataa
     3241 cggtagccgt attcatattc caagtgataa cctgatgcga tggccgcgcc aaaacaaaag
     3301 gctaaaagcg cgcaaatgcc caatgtattt tttaatgtct gattcatatt ttttattcgg
     3361 gcggattgtt tcatacgatg ccgtccgaat atcccattat ttacgagtta acaaagcctg
     3421 tccgacgatt tcaagatcgg aaaaacggtg cttcacgcct tgtacatcct gatagttttc
     3481 atgccctttg ccggcaatca ggatgatgtc gtttgcggcg gcttgttcaa ccgcataacg
     3541 gatggcggcg gcacggtcgg cttcgacgca ttcgggcgcg ggaacggcag gcaggatgtc
     3601 gttgatgatg tcgtgcggat tttccaaacg cggattgtcg ctggtgacga cgactttatc
     3661 cgcgccctgt acggctgccg cgcccatcag caggcgtttg ccgcgatcgc ggttaccgcc
     3721 gcaaccgaat acgcaccaca aagccgcacc ctgcggtttg atttcctgca aggtggcgag
     3781 tgctttttcc aatgcgtcgg gcgtgtgggc ataatcgaca acgaccaaag gtttgccgct
     3841 gttcataata caatccatac ggcctgaagc aggacggatt ttttccagta catccaatac
     3901 cttatccagc ggatagccgt tggcgcaaag caaagcgaca caggcggcga gattttgcgc
     3961 gttgaaccgt ccgagcaggc gcgtgtggca tttcccttcg ccccacgggg ttcggaatac
     4021 ggcttctatg ccgtctgaag aggcggtaaa gtcggtaatg cggatgtcgg cgtgttcgct
     4081 gaagccgtaa ctgtaaacgg ccaaatcggg gcagtctttt ttcagacggc ctatgagttc
     4141 tgcgccgtat ccgtcatcca cgttgatgac tgcgtgtttc aagccgtgcc agtaaaacag
     4201 gcgcgacttg atggcaccgt aggcttccat cgtgccgtgg tagtcgaggt ggtcgcgggt
     4261 gaggttggta aagattgcgc tgcggaatga cacgccgttg acgcgcgact ggtcaagccc
     4321 gtggctggag acttccatcg cggcgactgt tgcgccttgt tgacggaaac ggtagagcag
     4381 ggtttggaca tcgacggggg cgggtgtggt atgcgtggtt tcttccaatg caccccaaaa
     4441 gccgttgccg accgtgccga caatggcggt tttttcgccc aacaaatcgg cagcttgcgc
     4501 cagccattgc gtgatggagg ttttgccgtt ggttccggtt acgccccaga atttgagacc
     4561 gtctgaaacg ttgccgtaaa cttgcgccgc caatatgccg gcacggtgtt tcaaatcttt
     4621 gatgccttga ttggggactt tccattcggg attccacgca aatttgccgt cgtcgtccca
     4681 aaaaacaaaa gccgcgccgt tggcaacggc ggcggggata taactgcgtc cgtcggcata
     4741 ttcgcccgga caggcaacga aaatatcgcc ttgtttgatt tggcggctgt ctgaatgcaa
     4801 caaacgccct gccgcgtttg cacacgacag ggtcgggatg ccggtttcag ccaaaggggt
     4861 taacttgctg aacataaaac aatctcgttg atactcggat taagacggtg ttttgacggc
     4921 tgcggcggtc aggggcttgg tcggggaaat gcccaagatg ttcagactgc cgcccataat
     4981 ttttttgaag ggcggccctg ccactacgcc gccgtaataa ccgtgggcag tcggttcgtc
     5041 aatggttacc gccacaatca cacgggggtt tttggcgggg gcaaaaccga taaaggtagc
     5101 gatgtgtttg ttgtcggcat aacgcccgtt gacgaacttg cgcgccgtgc cggttttcgc
     5161 gccgacatcg aaaccgtcca ccgcacccgc cgtaccggtg ccgcccggct cggttacgga
     5221 aaccatcaga ttgcgtacct cgcgcgcggt cgattctttg aatatgcgtt tgccttgcgg
     5281 cgcaaccgcc tgtttttcaa agctgaccgg cagtaaaacg ccgtcgtgcg tcagtgcggt
     5341 ataggcgcgc gccaattgca gcaggctcaa ttgcaggccg taaccgaaag acatcgtcgc
     5401 ctgttcaata ggccgccacc tgcgccaatt tctcaacaaa cctgcagttt cgcccggaaa
     5461 gcccgagtgc atacgcacac cgatgcccaa ctcatgatag aagtcataca tttcttcggc
     5521 accgaaacgc gcagacagtt tgcttgtgcc gacgttggac gatttctgca tgatgccgcg
     5581 cacatccaaa gaggggtaaa catgggtatc gcgcacggga gacggtccga ttttataagg
     5641 ctgcgtattc agccgttcgt tcaaatcggt tttgcccgca tccaatgctt tcgcaatcac
     5701 aaacggtttg attgccgaac cgggttcgat catatcggtt acggcacggt tgcgccgctg
     5761 ttcgctgtct gcccggccgg gcctgttggg atcgtaggcg ggcgtattgg ccaaggcgag
     5821 gatttccccc gtgcgggcat ccaaaaccac caccgttccg gcttttgcct gatggtattc
     5881 gaccgccttg ttcaactctt cataggccaa ggtctgaatc ctctgatcga gggaaaggat
     5941 gatgtctttg ccgtttttcg gggctttatt gcgcggggag tccaagctgt ccacaatatt
     6001 gccctgccgg tcccgcaaaa cgacttccgc gccgtcttcg ccatgcaggc tgtcttcaag
     6061 cgaaagttcc aaaccttcct gacctttgcc gtcaatatcg gtaaatccga tgacgtgtgc
     6121 aaacaggttg cccatcgggt aatggcgttt taattctttt tcaaatacaa agttttccaa
     6181 acccaaggct ttgacctctt cggcaacctt gggatcgagc tgccgcttaa tccagataaa
     6241 cgacttgcct ttctgttcga gcttgttcct caaaacatca accggcacat cgacaagctc
     6301 ggacaggcgt tccaattgtg cggcagacgg catttccttc atctctttag gcacggcaaa
     6361 cagggactcc gtcggcgcac tcaacgccaa aaccgcaccg ttccggtccg aaaccgtacc
     6421 gcgtgtagcc ggcaatgttt gagtccgcac aatccggttg tcgccctgtt ctttcaaaaa
     6481 gttatacgtt accgtctgca gatacagtcc gcgagcaatc agaccggcaa acaagaccgc
     6541 tattgccatc aggacgaagc tgatccgtcc gttactggtc atcggctttt tgacctgctc
     6601 ttctttgggc agcatccgag gcttatattc gctcttaatc aacattttta cttctcgtta
     6661 ttattatcct gacgcaggaa tccgattccg gcacacaggc tgcttctatc tttgatgcgc
     6721 caccataaag gtattgcccg aaaccggcgg atggaggttt tgtttttctg ccgccgccct
     6781 gatcgcttcg tggttcgcca aacgcgcctg ttgcagcctc atttgcgcat aatcctgctc
     6841 caaggcgatt tcctgttttt tcgccttgtc caaagctgtg aagttgagcc tgtactggtt
     6901 ttgctgcatc acaacggaaa aagcggaaac gcacaccgca agcagcagaa ggaaattcaa
     6961 tttgttcatt gccattcaga cgtttttctc tgtgattgtt ccggtatcga accggcagtc
     7021 cgctccgcca cacgcaaaac cgcacttctc gccctcggat tggcggcaat ttccgcctca
     7081 cccggcttta atgccctgcc cacgattttc aggggcggct cgggcaaatc cgcttccctg
     7141 accgccgccc agcgcggcag gggcgcgtgt tgcgaatatt ttttgacaaa ctgcttcaca
     7201 atgcggtctt ccaacgaatg gaaagcaatg accgccaaac gtccgccctc tttcagacga
     7261 cacatgacct gcggcaatac tgcccctact tcttcaagct cgcggttaat aaagatgcgg
     7321 accgcctgga aggtgcgcgt cgcaggatcc tgcccccgct cgcgagtacg gacgttttgt
     7381 gccacgatct gcgccagctt gcgggttgta tcgattggac tttccgcccg ttgcgcaaca
     7441 atggcgcgcg caatctggcg gctaaaccgc tcttcaccat aattcttgat tacctcgtgc
     7501 aaatcctgtt ccgacgcaac cgctatccac tctgcggcag acataccgcg cgtcgtatcc
     7561 atacgcatat ccaaaggggc atcgaaacgg aagctgaaac cgcggctgcc gtcatcgatt
     7621 tgcggggacg aaatccccaa atcaaacagc gcaccgtcca ccttgccgat acccaaaccg
     7681 tccaatgccg tctgaaacga agcaaaaccg ccatgcacga caccgacccg tttgtccgaa
     7741 cgcgccagct cttctgccac agcaatcgcc tgagggtctt tgtcgaaaac aatcaaccgc
     7801 cccgcatcgc ccaaacgcga caaaatcagc cgggaatgcc ctcccctgcc gaacgtaccg
     7861 tccacataga caccgtcttc gcgcacggca agcgcatcca ccgcctcatt cagcaagacc
     7921 gtgatatgcc ggtaactttc tgctccactc acaattgcaa atccgtctga cccaactgga
     7981 aggcaagttc gtcaggatcg tcatccaaag cctgaaccat ctcagcctcc cactgctcgc
     8041 gaccccaaag ctccaaacgg ttggcacgac cgaccaaaac gacttcacgg tcgaaatcca
     8101 cccttttcct cagtccggca ggaagcagca cccggccggc gctgtcccat tccaaaattt
     8161 ccgcgttatg cagcaaaaga ttttgaaacc gccgcaaaac agggttatcc gccactttta
     8221 agtttaaaag ttgcgccgca accttttccc actccgcaac agggtacatc aacagcttgt
     8281 gtttcgactc gagcgttacc actacggcag gcgtatagag gcgcgacaga atgtcacgga
     8341 atttggcagg aacagccaac cgccccttac tgtcgatgct taattcgtgt gcgccgccga
     8401 acatgacatg tcccaagccg aaatcaaaat cacaagggta aaagagacac tttgccccac
     8461 aattccccac caatcgacac tataagaaat tttaaacact cggtcaaatc agggcatgaa
     8521 aactcattaa catatctgaa atttttattc cttttaaaaa caataagata aaaaatgacg
     8581 acaacggcac ggcgggtgcg gtacagaata atcgaaccaa taaacaacta tatatgatta
     8641 atttaataat ataaacacaa tatatagtat taagataaag ccatgacagc acccgtacca
     8701 acgtgtaata tgtcgggaaa tccaataaat ttacacaagc taacacttat catgcccctc
     8761 cccctcccct ctcccgaagc acggcaattc tcgctcaaac tgcaaaccct cattgccgaa
     8821 gaaatcggca aacacggcaa ctggattcca ttttcacgtt ttatggaatt ggttttatac
     8881 gctccgcaat acggctacta caccggcggc agccataaaa tcggcaatac cggggatttt
     8941 attaccgcac cgaccctcac ctctctgttt gcacagacac tggcacgcca acttcaagaa
     9001 cttctatccc aaacggcggg caatatctat gaattcggcg cgggaaccgg acagcttgcc
     9061 gccgatttgt tgggcagcat ttcggacggc atcagccgtt actatattat tgaaatatcg
     9121 ccggagctgg cagcacgtca gaaaaacctg attcaagcac gcgcaccgga agcatctcaa
     9181 aaagttgtcc acttgaccgc acttcccgaa gcgtttgacg gcatcatcat cggcaacgaa
     9241 gtactcgatg ccatgcctgt cgaaatcgtc cgtaaaaatg aaggcggctc attcgagcat
     9301 gtcggcgttt gcctagataa tgaccgtttt acctattcgg cacgaccgct gcacgactta
     9361 cagctatctg ccttggcttc cctctatttt cctcaaacag attatcccta caccagcgaa
     9421 ctacatccgc aacaatatgc ctttatccgc acccttgcct caaggcttga acgcggaggt
     9481 atgatattca tcgactacgg ttttgatgca gcgcagtatt accaccctca acgcaatcaa
     9541 ggtactctga tcggacacta ccgacatcac gttatccaca atccttttga cttcatcggg
     9601 ttggccgacc tgaccgcgca tgtcaacttt accgacattg cccaagccgg tacggatgca
     9661 ggactggatt tgacaggcta tctgccccag tcccattttc tgctgaatct gggcataacc
     9721 gaactgttgg cacaagtggg aaaaacagac tcgcccgcct acatccgtga agctgctgcc
     9781 gttcagaaac tgattgacca gcatgaaatg ggagaattgt ttaaagtcat cgcattcggt
     9841 aaaaatatcg gcgtcgactg ggcaggattc cgcttcggcg acatctgcca caaactctaa
     9901 ccctcatgcc gcctgaatcc gcttcagacg acataaactt tttaacattt aaaaacagtc
     9961 aactaattca aaattaaaaa atacggcttg tcaaaaaaac agaaaaacat ataatagtgt
    10021 cttcacgaaa cggcgaatta gctcagtcgg ttagagcaga ggaatcataa tccttgtgtc
    10081 cggggttcga atccctgatt cgccaccaaa ttttcggggg tatagctcag ttggtagagc
    10141 gcttgcatgg catgcaagag gtcagcggtt cgatcccgct tacctccacc agataaaaaa
    10201 gcacagaccg taaaaaggta tgtgcttttt tattacctga ttgccgggaa ataaagaaca
    10261 aaccactgcc ttcaaaacag gcaatcgact ttaaacctat cgccccgcct gtcctgattt
    10321 tatagtgaat taaatttaaa ccggtacagc gttggctcgc cttgccgtac tatctgtact
    10381 gtctgcggct tcgtcgcctt gtcctgattt ttgttaattc actatatcag cccgccagac
    10441 aaacccgacc cgaataatgt cttcaggtcg ggtttatggt ttcattccca acttatccag
    10501 cccgacaacc acaatattat gatgacgatg ccgaaaacaa tgcggtaata ggcaaacggg
    10561 atatagtttt tcttggaaac aaacttcagc agtgctttga ccgctactaa accggaaaca
    10621 aaagcggcaa taaagcctat cagaatcaaa ccgacatcat gcagggtgaa aaaccggtaa
    10681 tgtttcagga catcataagc cgttgcggca accatcatcg gaacggccaa gaaaaatgaa
    10741 aactccgttg ccgttttccg ctcgattccc caaagcatcc cgcccataat cgtactaccc
    10801 gaacgggacg tacccggaac cagtgcaaac acttgggcaa cgccgatcat caacgcatca
    10861 atcggacgca atgcatcaac atcggcaatt ttaggctctg cccggctttg gcgtttctcc
    10921 acccacaaaa taaaaaaacc gcccaaaacc agcatgaccg ccacactcaa aggattgaac
    10981 agatgttctt taatctgttt gccgaacaac agccccatca cggcggcagg tataaaagca
    11041 atggcaagat tgaggacgaa gcggttggct ttccggtctt ttcccacgcc atgcaacaca
    11101 ttgctgaaac gctgccggta ttcaaacact accgccaaaa ctgcaccgag ctggatggca
    11161 atttcaaaaa ccttgtgatt gctgtgaaaa tcaatcagat tgccgaacac aatcaaatgt
    11221 ccggtgctgg aaatcggtaa aaattcggta aaaccttcta ccaagcccat catcagggct
    11281 ttcaggacaa tcagaaaatc cattgcttgc gcttctttcg gatacgggag ttcggctatt
    11341 tctgtacagc aggggtctga cgcttgcgtt cttccctgac tttggcaacc aattctttaa
    11401 tcgtatgaac gccgccgtca aagccattat tgaagataac gcggtatttt ccgccgacaa
    11461 taacggtcgg cgtgctgtca ataccgtatt gttccgtcag tttctgcatt tttaatgcgg
    11521 cggcggcagc ttcgggggaa tcataggcgc gcatcagttt tttgccgtca aagccttttt
    11581 gagacaaagc ccattttccg gcaacagccc tgttttccaa gcggattttt tgttcgtaaa
    11641 ctgctttaaa cacagcaggg tttgcctgat atttcaaacc cgacaaattg acggcagccg
    11701 ccatcctggc cagaccgagc atttcaggct gccagaccac gtgctccgtc ctcaaatagg
    11761 catcagacgg caatgccttg cccaatttca ataacaaagg atcgaaatga tggcaatgta
    11821 cgcagaaata gccgaaaaat tccaaaacct caattttgcc cgactgttct tgaggaatgg
    11881 gtttatccaa cacaagatag tcttcccttt ccgtcagggc atatgcctgc gcggacaaca
    11941 ctgccgacag cagcagcggc aacagatgtt tgagcttcat aattatttgc tttcgataga
    12001 acggatcagg ctggcgactt catgtttttt caactcgtcc tgcatttttt tcaccgcatc
    12061 ggcagacata ttgccgcttt gcacccggta aagcgtttta tgtcccgcct gataaccgac
    12121 caccttggaa gatatgccca agattgccag tttggcacgc tgtccttccg cgctccggcg
    12181 gtcggcatac gcgcccattt gcagataatg cgttgcttcc gccttgtcgg gcgttttcat
    12241 tttctgcact tctttggcag cggcactgcg cgctttttcg atgctgccgc tgttgaggat
    12301 ttgttccggg gtcggtttgg gtgcaacttt ttccttctcc gcctttttct cttcttttga
    12361 agcttttttc tctgtttctt tagatggttt taccgcttgt tttttaaccg tttcggcatc
    12421 tttcttctgc gctttttccc cgacggtttg ttcacgctct tccgtcagtg ctttcttgcg
    12481 cactgcctgt ccgtccgact tttcccgctc cggctcgtcc gccttttcct caacctcgtc
    12541 ggctttatcg gcagcttttt ccgcatccga ctgctctgcc tctttcgcag catccggttc
    12601 ggacaaggcg ttttgatcgg ccggttcagg ttggatgtct tccttaggct ggtttttcgg
    12661 tttcaggatt tccgtttctg caggctgctt cgacggaacc gggattttga acgcattttg
    12721 accgctctgg ttcagataaa acaaaatacc ggcaataatg accgtcgcca gtatcaaacc
    12781 gaagaaaaaa ccggacagac cttttccgga ttgggaaaat ttgttcataa acatacctta
    12841 atgtgtttca gacggcatta acgccgtttg cttacgggcg gatattctaa caatatcgcc
    12901 atatttgggc aaaacctgct tccattcccg ttcctataaa gcgcggcgga aacctatggc
    12961 tttcatgacg tggcttctgc cgacttcttc gtcgcccgcc aaatccgcca atgtacgcgc
    13021 cacgcgcatg atgcggtgga aactgcgggc ggagagggag agtttttcca gcaggctgcc
    13081 caatgcttcc tgcgcttctt tttggatgcg ggcttgggaa tcgagttcgc tgacgctcaa
    13141 agcggcgttg actttgccct gccgtgcgta ttgtttgtcg cgggcggcga taacgcgttc
    13201 caaaacggac gcgctgcttt cgcccgcttc ctgctgcatc agttcgacgg cggacaggct
    13261 ggggacttcg atggtcaaat cgatgcggtc gagcagcggg ccggaaatct tgctgcggta
    13321 acgcgcgacg ctttcgggcg tgcagcggca gggtttgacg ggatgcccga gataaccgca
    13381 cggacacggg ttcatggcgg cgacaagttg gaatttggca ggatagacgg cttggcgcgc
    13441 cgcgcgggaa atgtggattt cgccgttttc caacggttcg cgcaaaactt ccaaaacttt
    13501 gcggtcgaac tcgggcaatt cgtccaaaaa caaaacaccg tgatgcgcca atgaaatctc
    13561 gcccggacgc ggatccgaac cgccgccgac catagccgcc gcgctggcgc tgtgatgcgg
    13621 actgcggaaa ggacggttgc tgtcgagttg ttgctggtgg ttgggcagga gcgaacgcaa
    13681 tgcccaaact tcaaccaatt cgtcttcggt cagcggcggc aggatgccgg gcagccgttg
    13741 ggagagcata gacttgcccg ttcccggcgg acccatcatc aagaggctgt gtccgcctgc
    13801 ggcagcgatt tccaaagcaa ggcgggcagt gtgctgaccc ttgacatcga cgaggtcgag
    13861 caatttggtg ttttcagacg gcatctgagg aacttgacag tcggtttgca ccaaaggttc
    13921 gatgccgttc aaatgggcgg cgacttcgcc caaagagcgc gcgccgtaaa cggtaatgcc
    13981 gcgcatcacg gcggcttgtc ctgcgttttc ttcaggcaaa acaaatgcac gttttgcctg
    14041 cataccctgc cacgccatcg ccaacgcgcc gcgcacgggg cgcaacagcc ccgacagtgc
    14101 caattccccc gcaaactcgt attccgccag tttttcgggc gcaacctgcc ccgatgcggc
    14161 aaggatgccg attgcaatcg gcaaatcgaa acgccccgac tctttgggca ggtcggcggg
    14221 ggcgaggttg acggtaattt ttttggcggg gaattcaaaa ccgctttgaa taatggcggc
    14281 acggacgcgg tcgcgacttt cctttacttc cgtatcgggc agcccgacga tgttgaaatg
    14341 cggcaggccg ttggcaaggt gggcttccac ttcgaccaac ggcgcattca taccgctcaa
    14401 ggcgcggctg taaaccaagg caagcgacat atttcagacg gccttattcg ccggcttcgg
    14461 tttgctgcct gatttcggcg acggcttctt cggcagcggc ttcagccgct tccaatgctg
    14521 cccgttcggg attttgcgcg gcttcgagtt ttgccaaacg cgcttccaaa gccgccagtt
    14581 tggtacgagt tttgattaaa acctgctgct ggatgtcgaa ttcttcgcgc gtaaccagat
    14641 ccatacggtt gaacgcgccg cccagcatcg ccttaatatt tttttccaca tctttggcag
    14701 ggctgttggc gatggtttcg ctgattttcg aaccgacttc ttcaaaaagc tgcttgccga
    14761 acataatctg tatccttcct gaacatatca aattcaatcg gctattgtat aaggaaaaat
    14821 gccgtctgaa aacgggcggc ggataatcgg caaaacatac cgcgcctcct ttgcggttgc
    14881 taaattacac ctcaaaacac ctgccgcctg aaaacggatt tcatattccg cgcaacgcca
    14941 gattacaaga cactacaaaa caatatactg ttttaaatga tttttccgac gcgcatcgtt
    15001 tcaaactcgg ctttttaagc cattaagtgc tttgcaaaca acagaaattg ggttatcctg
    15061 aaacggatta tttacaattt catatagttt tattacatat cttattgtga ttgaagataa
    15121 tttatccgaa tccccctttc gggtatccgg attttccgtt gtacttttat tagaaaaaca
    15181 tttcaggcgc aagttgcttg caatttcaaa gcctgtaatt ctgaagtttt agacgaggaa
    15241 tagcacatga atcccatgta cattactttt gcaatctatc tggttgcagt ccttctcatc
    15301 gggcttgccg cctatttttc cacgcgcaat ttcgacgatt atattttggg cgggcgcagc
    15361 ctaggcccgt ttgttaccgc gatgtcggca ggcgcgtccg atatgtccgg ctggctgctg
    15421 atgggtctgc cgggcgcgat ttacctgagc ggtttgaatg aggcttggat tgccatcggc
    15481 ctcttggtcg gcgcgtattt caactggctc ttggtggcgg gccgcctgcg cgtgcatacc
    15541 gaatacgcca acaatgcgct gacgctgccg gattatttct tccaccgctt cggcgcgggc
    15601 ggacacttga tgaaagtggt ttccgcactg attatcctgt tcttcttcac gatttattgc
    15661 gcctcgggca ttgtggcggg cgcaaccctg ttccaaagcc tgtttgaagg tatgacttac
    15721 aatcaggcaa tgtggctggg cgcgggcgcg accatcgcct ataccttctt gggcggtttc
    15781 ttggcggtca gctggacgga tacgctgcag gcttctttga tgattttcgc cctgatttta
    15841 acgccggtga tggtctatct gggcttgggc ggcgcggaac agatgtctgc cgcgattcaa
    15901 agcgtcgccg caggcacggg caaagaatac ggcagcctgt ttgccggtac gaccgtcatc
    15961 ggcatcattt ccaccgccgc atggggcttg ggctatttcg gacagccgca cattttggcg
    16021 cgctttatgg cggccgaaag cgcgaaatcg ctggtatccg cacgccgcat cggcatgact
    16081 tggatggcgt tgtgccttgc gggcgcggtg gcggtcggtt atttcggcat tgcgtatttc
    16141 ggtgcaaatc ccgacaaagt ttcttctatg agcggcaacc acgaacgcat cttcatcgcg
    16201 ctttccaccc tgctgttcaa cccttggatc gccggcatca ttttgagcgc gattttggcg
    16261 gcggtgatgt ccaccctgtc ctgccagctt ttggtttgct ccagcgcgat taccgaagac
    16321 ttctacaaag gctttttgcg taaaaacgcg caacagtcgg aactggtatg ggtcggccgc
    16381 ctgatggtct tggcgattgc cgtgatttcc atcctgattg cttccgatcc gaacagcaaa
    16441 gtattgggac tggtgtctta cgcgtgggca ggtttcggtg cggcattcgg cccgattgtg
    16501 attctgtccg tattgtggaa acgcatcact gcctacggcg cgttgtcggg tatggttgca
    16561 ggtgccttaa ccgtggtcgt atgggcggaa tgggtgaaaa aaccggctca agcggcaggc
    16621 gaaagcggct tgttgacgat gtacgaaatc gtcccgggct ttattgtttg cttgatcgtt
    16681 gccgtgttgg tttccctgtt caacaaagaa ccttcgcgcg aaacccaaga acgctttgaa
    16741 aaagccgacg cagattaccg cgccgcccga taaacccctt tcagacggca cggcatcccg
    16801 gttcgtgccg tctgaacccg tttccgggtc aaacacaaat aaaccttgcc tgcatacagg
    16861 ccttatttgt gttttacccg tttcattttc aaagaaacgg aagcacctac cctgcccgca
    16921 tttgaagcct tgtatttcat ctgcggattt attacgatat taccgtattc agaccgcacc
    16981 gatgccgtct gaaccccccg aaaaactttg gagaatccaa aaaatgtttc attttgcatt
    17041 tccggcacaa actgccctgc gccaagcgat aaccgatgcc taccgccgta atgaaatcga
    17101 agccgtacag gatatgttgc aacgtgcaca gatgagcgac gaagagcgca acgccgcctc
    17161 cgagcttgcc cgccgtttgg ttacccaagt ccgcgccggc cgcaccaaag ccggcggcgt
    17221 ggatgcgctg atgcacgagt tttcactctc cagcgaagaa ggcatcgcgc tgatgtgtct
    17281 ggcagaagcc ctgctgcgta tccccgacaa cgccacgcgc gaccgcctga ttgccgacaa
    17341 gatttcagac ggcaactgga aaagccattt gaacaacagc ccttccctct tcgtcaacgc
    17401 cgccgcctgg ggcctgctga ttaccggcaa actgaccgcc acaaacgaca gacaaatgag
    17461 ttccgcactc agccgcctga tcagcaaagg cggcgcaccg ctcatccgcc aaggcgtaaa
    17521 ttacgccatg cgcctgttgg gcaaacagtt cgtaaccgga cagaccattg aagaagccct
    17581 gcaaaacggc aaagaacgcg aaaaaatggg ctaccgcttc tccttcgata tgttgggcga
    17641 agccgcctac acccaagccg atgccgaccg ctactaccgc gactatgtcg aagccatcca
    17701 cgccatcggc aaagatgcgg caggacaagg cgtttacgaa ggcaacggca tttccgtcaa
    17761 actctccgcc atacatccgc gctactcgcg cgcccaacac ggccgtgtga tgggcgaact
    17821 gttgccgcgc ctgaaagagc tgttcctttt gggtaaaaaa tacgatatcg gtatcaacat
    17881 cgatgccgaa gaagccaacc gtctggagct gtctttggat ttgatggagg cactcgtttc
    17941 cgatcccgat ttggcaggct acaaaggcat cggtttcgtt gtccaagcct accaaaaacg
    18001 ttgtccgttc gttatcgact acctgatcga ccttgcccgc cgcaacaacc aaaaactaat
    18061 gatccgcctc gtcaaaggcg cgtattggga cagcgaaatc aaatgggcgc aagtggacgg
    18121 cttgaacggc tatccgacct acacccgcaa agtccacacc gacatctcct acctcgcctg
    18181 cgcgcgcaaa ctgctttccg cgcaagacgc ggtatttccg caattcgcca cccacaacgc
    18241 ctacaccttg ggcgcaatct accaaatggg caaaggcaaa gactttgaac accaatgtct
    18301 gcacggcatg ggcgaaaccc tgtacgacca agtcgtcggc ccgcaaaact taggccgccg
    18361 cgtgcgcgtg tacgccccgg tcggcacaca cgaaaccctg ctcgcctact tggtgcgccg
    18421 cctgttggaa aacggcgcga actcgtcttt cgtcaaccaa atcgtcgatg aaaacatcag
    18481 catcgacacg ctcatccgca gcccgttcga caccatcgcc gaacaaggca tccacctgca
    18541 caacgccctg ccgctgccgc gcgatttgta cggcaaatgc cgtctgaact cgcaaggcgt
    18601 ggacttgagc aacgaaaacg tattgcagca gcttcaagaa cagatgaaca aagccgccgc
    18661 gcaagacttc cacgccgcat ccatcgtcaa cggcaaagcc cgcgatgtcg gcgaagcgca
    18721 accgattaaa aaccctgccg accacgacga catcgtcggc acagtcagct ttgccgatgc
    18781 cgcgcttgcc caagaagcgg ttggcgcagc cgttgccgcg ttccccgaat ggagtgcgac
    18841 acctgccgcc gaacgcgccg cctgcctgcg ccgtttcgcc gacctgctcg aacagcacac
    18901 ccccgcgctg atgatgcttg ccgtgcgcga agcaggcaaa acgctgaaca acgccattgc
    18961 cgaagtgcgc gaagccgtcg atttctgccg ctactacgca aacgaagccg aacataccct
    19021 gcctcaagac gcaaaagccg tcggcgcgat tgtcgccatc agcccgtgga acttcccgct
    19081 cgccatcttt accggcgaag tcgtttccgc attggcggca ggcaacaccg tcatcgccaa
    19141 acccgccgaa caaaccagcc tgattgccgg ttatgccgtt tccctcatgc acgaagccgg
    19201 catcccgact tccgccctgc aactcgtcct cggcgcaggc gacgtgggtg cggcattgac
    19261 caacgatgcc cgcatcggcg gcgtgatttt caccggctcg accgaagtgg cgcgcctgat
    19321 caacaaagcc cttgccaaac gcggcgacaa tcccgtcctg attgccgaaa ccggcggaca
    19381 aaacgccatg attgtcgatt ccaccgcact tgccgaacaa gtctgcgccg acgtattgaa
    19441 ctccgccttc gacagcgcgg gacaacgctg ctccgccctg cgtatcttgt gcgtccaaga
    19501 agacgttgcc gaccgtatgc tcgacatgat caaaggcgcg atggacgaac tcgtcgtcgg
    19561 caaaccgatt cagctcacta ccgatgtcgg ccccgtcatc gatgccgaag cacagcaaaa
    19621 cctgttgaac cacatcaaca aaatgaaagg cgttgccaag tcttaccacg aagtcaaaac
    19681 cgctgccgat gtcgattccg aaaaatccac gttcgtccgc cccatcctgt ttgaattgaa
    19741 caacctcaac gaactgcaac gcgaagtctt cggtcccgtc ctgcacgtcg tccgctaccg
    19801 cgccgacaaa ctcgacagcg tcatcgacca aatcaacagc aaaggctacg ccctgaccca
    19861 cggcgtacac agccgcatcg aaggcacagt acgccacatc cgcagccgca tcgaagccgg
    19921 caacgtttac gtcaaccgca acatcgtcgg cgcagtcgtc ggcgtacagc ccttcggcgg
    19981 acacggtctg tccggcacag gccccaaagc aggcggttcg ttctacctgc aaaaactgac
    20041 ccgcatcccc gaatgggttg ccccgaccct gagccaaatc ggacaggcag acgaagccgc
    20101 gctcaaacgc ctcgaagcac tggttcacaa actgccgttc aacgccgaag agaaaaaagc
    20161 cgcagcggcc gctttgggac acgcccgcat ccgcaccctg cgccgtgccg aaaccgtcct
    20221 taccggcccg accggcgagc gcaacagcat ctcatggcac gcgccaaaac gcgtctgggt
    20281 acacggcggc agcctcctgc aagccttctg cgccctaacc gaacttgccg cctccggcgt
    20341 acaggcagtg gtcgaacccg acagcccgct ggcttcctac accgccgact tggaaggtct
    20401 gctgctggtc aacggcaaac ccgaaaccgc cgacatcagc cacgttgccg ccctgtcgcc
    20461 tttggacagc gcgcgcaaac aggaacttgc cgcccacgac ggcgcactca tccgcatcct
    20521 cccttcggaa aacggactcg acatcctgca agtgtttgaa gaaatctctt gcagcgtcaa
    20581 caccacagcc gccggcggca acgccagcct gatggcggtt gccgactgat tttaccgaaa
    20641 tccccgagcg cggcccgtga accaatgccg tctgaaaacc tttcagacgg ca
//
LOCUS       DAWFAN010000019        20370 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWFAN010000019 DAWFAN010000000
VERSION     DAWFAN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20370
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.019"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(142..2748)
                     /gene="acnD"
                     /locus_tag="WGO65_000471"
     CDS             complement(142..2748)
                     /gene="acnD"
                     /locus_tag="WGO65_000471"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ0326809.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRRPGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLQRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGREIRLKDIWPT
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGKAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSLARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIAAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVIGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(2826..3614)
                     /locus_tag="WGO65_000472"
     CDS             complement(2826..3614)
                     /locus_tag="WGO65_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ0326810.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPILLIFVALYFVFSPKLDGSKEGKARISFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIISMF"
     gene            complement(3620..4273)
                     /locus_tag="WGO65_000473"
     CDS             complement(3620..4273)
                     /locus_tag="WGO65_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ0326811.1"
                     /translation="MRLVGNFKGGGATKLENEDVSKVDKSSHSGIALGAHTEAPYHAA
                     TKIFNNHSPAPSSLVLTARYNPLSEPTTVIPLQPILEKLHYLDLLALTTENFDFTRSE
                     TFTSGKGTGGSKVSILEFNNDGTIGVKYNSYRFTTNVHAPQSVKDALNRFNELINNSE
                     QYKINLQPNTCMIINNTLALHCRDVIQDNRRLLIRIFGYKNNIEYISLEDDPVLAQG"
     gene            complement(4490..4672)
                     /locus_tag="WGO65_000474"
     CDS             complement(4490..4672)
                     /locus_tag="WGO65_000474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326812.1"
                     /translation="MDKFQSIINLTADAALFVQNKATEIATIGGCQAKGVREIASNAG
                     KTLLEKYLNSEQKDEI"
     gene            complement(4805..5959)
                     /gene="prpC"
                     /locus_tag="WGO65_000475"
     CDS             complement(4805..5959)
                     /gene="prpC"
                     /locus_tag="WGO65_000475"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ0326813.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTELCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSLLLYWYQYSHNGKRIEVES
                     DEETIGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(6044..6919)
                     /gene="prpB"
                     /locus_tag="WGO65_000476"
     CDS             complement(6044..6919)
                     /gene="prpB"
                     /locus_tag="WGO65_000476"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ0326814.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDTMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(7545..8855)
                     /locus_tag="WGO65_000477"
     CDS             complement(7545..8855)
                     /locus_tag="WGO65_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ0326815.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(8994..10172)
                     /gene="ftsZ"
                     /locus_tag="WGO65_000478"
     CDS             complement(8994..10172)
                     /gene="ftsZ"
                     /locus_tag="WGO65_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ0326816.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(10291..11535)
                     /gene="ftsA"
                     /locus_tag="WGO65_000479"
     CDS             complement(10291..11535)
                     /gene="ftsA"
                     /locus_tag="WGO65_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ0326817.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVTSCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(11621..12349)
                     /locus_tag="WGO65_000480"
     CDS             complement(11621..12349)
                     /locus_tag="WGO65_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ0326818.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYAYDGLPEKESEE"
     gene            complement(12339..13253)
                     /locus_tag="WGO65_000481"
     CDS             complement(12339..13253)
                     /locus_tag="WGO65_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ0326819.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(13366..14775)
                     /locus_tag="WGO65_000482"
     CDS             complement(13366..14775)
                     /locus_tag="WGO65_000482"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ0326820.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGANVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(14929..15996)
                     /gene="murG"
                     /locus_tag="WGO65_000483"
     CDS             complement(14929..15996)
                     /gene="murG"
                     /locus_tag="WGO65_000483"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ0326821.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(16000..17325)
                     /locus_tag="WGO65_000484"
     CDS             complement(16000..17325)
                     /locus_tag="WGO65_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ0326822.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(17451..18788)
                     /locus_tag="WGO65_000485"
     CDS             complement(17451..18788)
                     /locus_tag="WGO65_000485"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ0326823.1"
                     /translation="MTLENKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATKDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGKKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(18878..19150)
                     /locus_tag="WGO65_000486"
     CDS             complement(18878..19150)
                     /locus_tag="WGO65_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ0326824.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWFGKACQNGDQDGCDIYQRLKAGY"
     gene            complement(19265..20347)
                     /locus_tag="WGO65_000487"
     CDS             complement(19265..20347)
                     /locus_tag="WGO65_000487"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ0326825.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         4946 a         5516 c         5043 g         4865 t
ORIGIN      
        1 aaggtttcct caattccact ttctggattc ccgcctgcgc gggaatgacg atttacagta
       61 gatgcctgcc atatcgagaa ttacgtgaat gagcaaaatg ttgaacccga cccacgctac
      121 gtcttgcttt tcagacgacc tctaagccgc gttcccttcc aaaaaatcct gtgcaaaccg
      181 ttgcaacacg ccgccggctt catataccaa tacttcttct gcagtatcga ggcggcaggt
      241 aacgggaact tcgacggttt ctccgttttt acggtgaatc acgagggtca ggtcgcagcg
      301 cggtgtacgt tcgccgataa cgtcgtaggt ttccgtgccg tccagttgca gggtgtggcg
      361 gttggtgccg ggtttgaatt gcagcggcaa gacgcccatg ccgatgagat tggtgcggtg
      421 gatgcgctcg aaaccttcgg cggcgatggc ttccactccc gcgagccgca cgcccttcgc
      481 cgcccagtcg cggcttgagc cttgaccata gtccgcaccg gcaatgatga tgagcggctg
      541 tttgcggttc atataggttt cgatggcttc ccacatgcgc atggtttcgc cttcgggttc
      601 gacgcgggcg agcgagcctt ggcgcacgct gccgtcttcg tttttcacca tttcgttaaa
      661 cagtttcgga ttggcgaagg tagcgcgttg ggcggtcaag tggtcgccgc ggtgggttgc
      721 gtaagagttg aagtcttctt caggcaaacc cattttcgcc aaatactcgc ctgcggcact
      781 gacggccaaa atcgcattgg acggcgagag gtggtcggtg gtgatgttgt cgggcaaaat
      841 cgccagcgga cgcatacctc ttaatgtgcg ttcccctgcc agcgcgcctt cccagtaagg
      901 cgggcggcgg atgtaggtgg acatcggacg ccagtcgtat agcgggcttg gggctttttg
      961 cgctttgccg gtgtcgaaca tcggtacata cacatcgcgg aactgctgcg gtttcacata
     1021 ttcagcaacg acggcatcga tttcttcgtc ggtcggccag atgtctttca ggcggatttc
     1081 gcggccgtct gcaacgccga gtacatcgtt ttcaatatcg aaacggatgc tgcctgccag
     1141 cgcgtaagca acgaccaacg ggggcgaagc gaggaaagcc tgtttcgcat acggatgaat
     1201 acggccgtcg aagttgcggt tgcctgacag tacggcggtg gcgtacaaat cgcggtcgat
     1261 gatttctttc tggattttcg ggtcgagcgc gccgctcatg ccgttacagg tggtacatgc
     1321 gaaggcaacg ataccgaagc cgagtttttc catttcaggc agcaggcctg cttctttcaa
     1381 atagatttcg gctacttttg aacccggggc aaacgaagat ttcacccaag gtttgcgttg
     1441 caagccgagg cggttggcat tgcgtgccaa cagcgcagcg gcgacaacgt tgcgcggatt
     1501 ggaagtattg gtacacgaag taatcgcggc aataatcact gcaccgtcag gcatttggcc
     1561 gtctgaaggc tcttcgtaag gcttcgccag ccctttcgcc gccaaatcgg cggtcgcaaa
     1621 acgggcatgc gggttacttg ggcctgccat attgcgcgtt acgctgctca aatcaaattt
     1681 caaaacgcgc ggataaacgg cggttttcaa ggcatctgcc cataagcctg cggttttggc
     1741 gtaggtttcc accaatttca cctgcgcgtc gtcgcgtccg gtcagtttca aataatcaat
     1801 ggtttgctca tcaatagcga acatcgcggc agtcgcgccg aattcagggg tcatattgga
     1861 aatagttgcg cggtcgccga tggacaggct tctcgcacct tcgccgaaga attcgacaaa
     1921 tgccccgacc acgcgttctt tgcgcaagaa ctcagtcagt gccaacacga tgtcggtggc
     1981 agtaatgccc ggccggcgtt tgccggtcag ctcaacgccg acaatatcgg gcaggcgcat
     2041 catggacgcg cgtcccagca ttacggtttc cgcttccaat ccgcccacgc ccacggaaat
     2101 cacgcccaat gaatcgacgt gcggcgtatg tgagtcagta ccgacgcagg tatcggggaa
     2161 cgccacgccg tttttgactt ggacgacggg cgacattttt tctagattga tttggtgcat
     2221 gatgccgttg cccgccggaa tcacgtccac attttcaaat gcggtttttg tccagttgat
     2281 gaagtggaaa cggtcttcgt tacgtctgtc ttcgatttgg cggtttttgc ggaaggcatc
     2341 ggggtcgtag ccgccgcatt caacggccag agagtggtcg acgatgagct gggtttgcac
     2401 caccggattc actttggcag gatcgccgcc tttttcggca atcgcatcgc gcagacctgc
     2461 caaatccacc aacgcggtct gccccagaat atcgtggcac accacccgcg ccggatacca
     2521 aggaaagtcg atttcctgtt ttccctcaat cagctgaccc agccagcttt gcagcgtcgg
     2581 caaatcgact ttgtccgcgc ggttgaccaa attctccgcc aaaatgcggc tcgtgtaagg
     2641 cagcttgtcg taagagccgg gcttgatgtc ctcacacgcc gcacgcgcgt cgtagtattc
     2701 caaatccgta ccgggcagcg gtttgcggta acgttggttg gcagccatgt cggttctcct
     2761 ggggatctgt ttttcttgtg gtttgaggtt tcagccgacg ttttgaaggg gtcgtctgaa
     2821 agggtttaaa acatcgaaat aatcatctga tacagcggat ttctctcgtc tatcaacaat
     2881 ttcacagcca tcgaaatgct gatgacaatc agcagcggct taatcagctt cgaaccgaag
     2941 cggacggcaa atctcgcacc taaattcgca ccgacaaacg caccgaccgc catcgttgcc
     3001 gcaatcggga aaataatcga accgtgcagc aggaataccg atagcgaacc aagattgcag
     3061 gcaacgttcg ccaatttggt gtaagacatc gcgttcaaca gcttgcagcc gagcaaaaca
     3121 ataaaggcaa tcagaaaaaa cgagccgaca cccggtccga acacaccgtc gtaaaaaccc
     3181 aaaagcggtg caaccgtcag cccgaataga aaaaaagata ttctggcttt gccttcctta
     3241 ctgccgtcga gcttgggcga aaacacaaaa tacagcgcga caaatatcaa caaaatcggc
     3301 acgaccgcca gcagaatatc tttggaaacc aagctgaccg ataatgcacc gaccacgccg
     3361 cctgcaaacg atgctgccgc aatcgggaga cctttcttcc aatcaatcaa acctttgcgt
     3421 gcaaaagaaa ccgtagccga aaacgtagca gcggctgctt gcagcttgtt ggtggcaatt
     3481 gccgacacgg gaggaatacc tgccaacaag agtgcaggca gcgtaatcaa accaccccca
     3541 cccgcaatcg catcgataaa tccggcaatc atcgcaacca aacccaaagc gagtattata
     3601 tataaatctt ccatgtttct tatccttgag ccaatacagg atcatcttct aaagaaatat
     3661 actcaatatt atttttatat ccaaaaatac gaattaacaa tcttctgtta tcttgaataa
     3721 catcacggca atgcaaagcc aacgtgttgt taatgatcat acaagtgttt ggttgtaagt
     3781 tgattttata ttgttcagag ttgttgatta attcattaaa tctattcaaa gcatctttaa
     3841 ccgattgggg tgcatgcaca ttagtagtaa atctataaga gttatattta acaccaatag
     3901 ttccatcgtt attaaattca agtatagata ctttacttcc ccctgttcct tttcctgacg
     3961 taaacgtctc actacgagta aagtcaaaat tttctgtcgt taatgccaat aaatccaaat
     4021 aatgcaactt ttccaaaata ggttgtaatg gaattactgt tgtcggttct gatagcggat
     4081 tgtatcttgc agttaaaaca agagatgatg gtgcggggga gtgattatta aaaatctttg
     4141 ttgccgcatg gtaaggagct tcagtatgcg cccctaatgc aattcctgaa tgtgaacttt
     4201 tatctacttt gctaacatct tcattttcaa gttttgttgc tccacctccc ttgaaattac
     4261 caacaagtct cattattttc ccatgattat caatattata agcaaatgca aattgctcta
     4321 ctgttgccaa taaaatctga ttagatgcag ccaatttcaa acattctaag ttttcttcaa
     4381 gctcttccat ttttggtaaa gaacttggaa tgctaattat tgaattatca atgtttttat
     4441 cataaagatt attaaaaata actacacaag ttttttgatg aatgaatttc tagatttcat
     4501 ccttttgttc tgagtttaaa tatttttcca ataaggtttt ccctgcatta cttgcaattt
     4561 ctcttactcc ctttgcttga cagccaccta ttgttgcgat ttccgttgct ttattttgaa
     4621 caaacaaagc agcatcagca gttaaattaa taattgattg aaacttatcc atttaataca
     4681 tacccttttt attaggttaa atttttaaac ttatttcaga caacctttcc aataaggcaa
     4741 ggtcgtctga aatcctcagc tttgcatacc gaaataaaga acaaactgct attgcattct
     4801 tcaattatcg ttcttcaatc tccacaaacg ccaaatcttc tggacctgtg tagtttgcgc
     4861 tcggacggat gattttgccg tctttgcgtt gctcgagaac gtgtgcgctc caaccggttg
     4921 tacgggaaat tacgaacagc ggtgtgaaca tagcggtcgg tacgcccaat ttttggtagg
     4981 aaacggcaga gaaccagtcc agattcggga acattttttt ctcttcccac atcacgcttt
     5041 ccaaacgttc ggcaatgtca aagaggcgca tatcgccggt ttctttgctc aaaccgcgtg
     5101 ccacttcttt aatgacaacg ttgcgagggt cggaaatggt gtacaccgga tgaccgaaac
     5161 cgatcacgat ttctttgcgg ccgatgcgtt cgcggatgtc ggcttcggct tcgtcggcat
     5221 tgcggtagcg tttttgaata tcgtaagcca cttcgttcgc gccgccgtgt ttcggacctt
     5281 tcaacgcgcc gattgctccg gtaatgctgg agtacatatc agagcctgta ccggcgatca
     5341 cgcgggcggt aaaggtagaa gcgttgaact cgtgttcggc atacagaatc agtgaaacgt
     5401 gcatggcttt gatgtgtgat tcgcttgggc gtttgccgtg caacaggtgc aggaaatgac
     5461 cgccgatggt ctcttcgtcg ctttcaactt caatgcgttt gccgttgtgt gaatattgat
     5521 accagtacag caggaggctg ccgaggctgg caatcagttt gtcggcgatg tcgcgcgctt
     5581 cactttccgg atggctttca cgttcaggat gaacgcagcc cagcatggat acgccggtac
     5641 gcatcacgtc catcggatgg gtatgtgcag gcaggctttc caaaacttta atcacacgga
     5701 taggcaggcc gcgcatggat ttgagcttgg ctttataagc ggccaactcg aatttattgg
     5761 gcaaatgacc gtgaatcagc aggtgggcaa cttcttcaaa ctcgcatttt tgtgccaaat
     5821 ccaagatgtc gtaaccgcga tagctcaaat cgttgccggt acggccaacg gtacacaatt
     5881 cggtattacc ggccgcaacg ccagaaagcg caacggattt tttaggtttg aaagtcgggg
     5941 tttgagtagt ttcagtcatg gtatttctcc tttgtatttt tatgggtttt atttttcaga
     6001 cggccgatgc ggatttgttg aaaggccgtc tgaaagcggt aaatcatttt tgaaacaatt
     6061 tatccagttt ttgctcgaag gcatgatagt tcagatgctc gtacaattcg gcacgggttt
     6121 gcatagtgtc aactactgcg gcctgagtgc catcgcgcat aatcgcttcg taaacattca
     6181 gagcggcttt gcttgctgca cggaacgatg acagcggata cagcaccagc gacacgccgt
     6241 tttcagccag ctcgctttgg gtataaagcg gagtggaacc aaactcggta atgttcgcca
     6301 acacgggcac tttcaccgca tctgcaaatt ggcggtacat gttcaaatcg gtcatggctt
     6361 cagggaaaat catgtccgca ccggcttcga cacaagcttg ggcgcgttcg atagcggcat
     6421 ccaaaccttc taccgccagc gcatcggtac gcgccataat cacgaagttc tcatcaacgc
     6481 gcgcatctac ggcagctttg atacggtcga ccatttcatc tttagataca atggctttgt
     6541 tcggacggtg gccgcagcgt ttttgcgcta cctgatcttc gatgtgaacc gctgcaacac
     6601 cggcgcgttc aaagttgcga atggtacggg caatattgaa tgcaccgccc caacccacat
     6661 cgatgtccac cagcagaggc gtatccacgt tgtccgtaat gcgtcgtgcg tcgatcagca
     6721 catcttccat tgtggtaatg cccaaatcag ggataccgca agaacaggct gccacgccgc
     6781 cgccggacag atagatggct ttgaaaccgc tttgggtggc caatcgtgca aaataagcat
     6841 tgacgcaacc ggcgacggca agcggattcg attctttcac ggcttggcgg aaacgtgctc
     6901 cggcagagtg ttgactcatc atatttctcc tttatagact ttttttcagt atggacaggc
     6961 ttccatcaca ttcggacggc aaaacacagc catccgacgc gtcgggcatc ccaatccaat
     7021 cattaaaaat atatgggaaa aattatctta ttgatattta aaacgaatca aagaaaaaca
     7081 gcagaccgtt cggaattatg cggcaaaacc gcagacaaga agaaaacaag gggattattc
     7141 agaaaagggg aaaacatctg aattggtttc atagtaatgt tcctttgtag tgttatgtag
     7201 ttttattttt cgataatcta aaacacaaga ctgaaaatgt caaacatttt taaggagact
     7261 cattacataa ttttaatatt ttattttaat tccatgatta aactagatta attttgaatt
     7321 atttgtttca ataaaattgt caggaaatca agaaaaattt tcgataatca aaatcctgaa
     7381 aacttataaa atattttata cataaaaaca atgtattatt tttaagcaat ccgatgtatt
     7441 ttattatgta tttgattttt atataattta ataaaaatta aatatatcgg caaacaaaat
     7501 gccgtctgaa aacctttcag acggcatttt taatatcgaa tcaatcagcc caaataccag
     7561 aatttcagtg cccacaatac ggcaacaatc cataccatag gcggaacgtc tttggtgcgg
     7621 cggcataaaa gtttaaccac ggcatagctg atgaagccga aggcgatgcc gtctgcaatc
     7681 gaataggtaa acggcatgaa gacaatggtc aggaatgcgg gtgcggcttc cgtcatatcg
     7741 tcccaatcaa tatccctcgc actgcggagc atctgcgtgc cgacataaag cagggcgggc
     7801 gcggtggcaa aagcgggaac acttttcgcc aaaggtgaaa acatcaggca ggcgagcatc
     7861 aatacgccga cggtaaccgc cgtcaggccg gtccgtccgc ctgccgatac gcccgccgcg
     7921 ctttccacat aaggcgtggt ggaagaagta cccaaagccg cacctgccac aatggcggta
     7981 gagtctgcaa gcagtgcgcg tttcaggcgg ggcagcttac cgtccaccag cagcccggca
     8041 cggtgggata tgccgaccag cgttccggta ctgtcaaata gatcgaccaa gaagaagacg
     8101 aaaatcacac tgaccatgct gacggtaaac aggccttcaa aatccatctg cataaaagtc
     8161 ggcgcaatgc tcggtacttc gccgatgatg ccgtgaaatt cattcaaacc catcaggctg
     8221 gcaatgacgg taatggtcaa gatggtgatg atgattgcgc cttgaacgcg gaaatgtccc
     8281 aatacgacca ccatagcaaa accgaacaat gccaacaacg cggacggctg atgaatatcg
     8341 cccaaaccga ccaaggttgc cggattggca acgataatgc ctgcgccttt cagggaaatc
     8401 agtgccaaaa acaaaccgat accggcagca atcgacattt tcaaacccat aggcagtgcg
     8461 ttgaccagca tttccctgac tttaaaaaag ctgaacagga taaaaatcag accggagatg
     8521 aacaccgcac ccaacgcaac ctgccaaggc acgcccatac ccttaacgac ggcaaaggtg
     8581 aaataggcat tcagccccat ccccggtgcg agtgcaatcg gatagttgcc gacaaaaccc
     8641 ataacaaaac agccgatggc agacgcgata caggtagcga cgaataccgc ccccatatcc
     8701 atgccggtct cgcccaaaat cagagggttg acgataacga tgtagcacat cgtcaaaaaa
     8761 gttgtcaaac ccgccatcaa ctcggtacgc accgtcgtac cgtttgcctt cagcttaaaa
     8821 atcccgtcca acagtgtttg ttttgaagtg tccatatctg aaagtctgta tcaagtggaa
     8881 aaatctgaat ccgccgaccg caaaccgtac gaaattgcaa catcttgaaa aacatacctc
     8941 ccaaaccgga caaaccgatt cgggaggcag caggtttacg ggcaaacagc acattatttg
     9001 tctgaattgt gttgacgacg caaaatcgca gggatttcaa agtcgtcaag tacggactga
     9061 ttgtcgaaat ccgcagcggt aaggttcatc gtgcggatac cgcgattggt gcggatcata
     9121 ccttcgacat tgtggctttg ctcctgtttg gacggagcaa ccgcttctac ctcccttgcc
     9181 ggaacaaaat cgaccgcgcc tttttctttc agaccggtag cgataatggt aatccggatg
     9241 gcatcttcgc tcatggtctc gtcttcagcc gcaccgaatt tgcattccaa atcgggatgc
     9301 gcgctttggt tgacgatttt catgacttcg gacaactcgg acattttcaa gcaacccgga
     9361 gcagtcgtaa tattgaccag cacaccgcgc gctccgtcca aggttacatc gtccagcagc
     9421 ggactggaaa tggcctggtc ggtcgccata cgcgcacggt cgataccttg ggcataaccc
     9481 gaacccatca tagcgatacc gcggttgctc atcacggttt tcacgtcggc aaagtcgagg
     9541 ttgatgattt cgctcgggca agttaccact tcggaaatgc ctgcgaccgc atcgcgcaat
     9601 acattgtcgg cggcacggaa ggcttcgcgc atcgttacgt cttcacccaa tgcagtcatc
     9661 agtttgtcgt tcgggatgat gatcagcgaa tcgacgtgtt ctttcaactg ttccaaccct
     9721 gcctgtgcga catggacgcg cttaccttca tatgcgaacg gtcgggtaac cacggcaacg
     9781 gtcagaatgc ccaaagactt ggcaatctca gcaacaaccg gcgcggaacc ggtaccggta
     9841 ccgccgccca taccggtcgt gataaacagc atattcgcac cgcgaatggc ttcttcaatg
     9901 gcttcccggt cttcctgggc tgccgcacgg ccgatatcgg gattcgcgcc cgcgcccaaa
     9961 ccgcgtgtca gattcgtacc caactggatt ctcttcgccg catggttttt tgccagagac
    10021 tgcgcatccg tattggcact gataaactcc acaccgcgca cattgttggc aaccatgtta
    10081 ttgattgcat tgcaaccgcc gccgcccaag ccgattactt taatcaccgc agggctgact
    10141 gccgattctg ccacgtcgta aacaaattcc attcaaaaac tcctgctcgc cccattcaga
    10201 ggacggttta aataaattat tattcattat ataagaagta tcttgctgct ggcaaaatac
    10261 ttctcacctg tcaaacggca atccacctgt tcagaagctg ttttcaatcc accgtttcaa
    10321 tcttgccaac aaaccgccgc ccccttccct ctcttgcact gcaccgtttt ccggctgcgg
    10381 caagtttcct tccagcttgc atgctgcatg aagcagcccg atagcggtag aaaagcgcgg
    10441 tgtgcggacg cggtcggaca aaccgcccat ttcttggggt gcaccggtgc gtacaggcaa
    10501 atcgaagatt ttttcggcaa attccacaat cccggtcatc atggacacac cgccggtcag
    10561 aacaataccc gcattcagca cttctttggg gaaacccgat ttttgcagct cgcccagcac
    10621 tacgccaaaa atctcctgaa tacgtgcact gatgattgct gccaaaacct tactggaaac
    10681 ctgacgcgat gtccggtcac ccacgcccgg aacttcaatc atctcaccca agccttccgt
    10741 atcgcatgat gtcacgccat aatggatttt aatgtactcg gcggcatcga gaggtgttct
    10801 caacgatttg gacaaatctt tggtaatcag attaccaccg gccggaatga cggacgtatg
    10861 gcggatggcg ccgttcatat aaacggcaat atcggtcgtt ccgccaccaa tgtcgatgac
    10921 gcatacgccg aggtcttttt catcttcagt cagcaccgcc tgcccgcttg ccaacggctg
    10981 aagcatgatc tgatcgcttt tcaagccgca ccgctcgata catttttgga cattctgcac
    11041 tgccgtactt gcaccggtaa tgatgtgcac ccgcgtatcc aaacgcacac cgctcatacc
    11101 gataggctcc ctcacgccaa gttgggtgtc gataatgtag tcttgaacca cagcatcgag
    11161 aattttttga tcgggcggga tattgattgc ctttgccgtt tcaatggcgc ggtcgatgtc
    11221 tgcctgcgtg acttccccgt ctttaatttt aaccacacct tgcgaattga gactgcggat
    11281 gtggttgcct gcgatacctg tggtaacgtg ggtaattttg gtatccgcca tcagctcggc
    11341 atcattgacc gcctgcctga tggcttggac ggtggcatcg atattggtta ccatgcccgc
    11401 gcgcaagccc cgtgaaggag cctgccccaa accgacgatg ttgattttgt cgtcatcttg
    11461 aacttccccg atcagtgcga ggactttaga cgtaccgata tccagtacgc tgatgtatct
    11521 ttgctgctgt tccattgttc gtctgctctt aaaactgatt gaaatttgcg tcgcaccgtt
    11581 tcagacggca cggccgtaat ctgtccgata cctgttccca ctattcttcg gattcttttt
    11641 cgggtaaacc gtcgtaagca tagcggactg aaaatccgtc cttatacctc atatccacat
    11701 aggataaccg atttttattt ttacgcaaca gatgctgcca cgcttcggta aaaagccgga
    11761 ggcgtttcat ctcgttttcc cgtccgagcc tgacggtgat gccgttgtcc aaaacgacaa
    11821 tccacgccga acgtgccgta taggtcatct ctttgatgcc caaaccctgt tttgccaaaa
    11881 cagtcgaaaa ttcgtcataa cggcggagca tttcggcaga cgttccttcc gcgcctctga
    11941 ataccggcat tccgggtctg tccaagcggg cttcaaaaac attgccttcg ccgtccacca
    12001 aggcatggtc gccccaacgc gcgaccggct tgcgctcggt caggacgacc tcaaccgtgt
    12061 cgggaaaacg gcggcgaacc atgaccgacg caatccacgg ataccggcgg taggcctcct
    12121 gtgcgccatt gatgtccgtc ctcaaaatat tcccatggat gtattctttc gccaaactgc
    12181 ccaatgcttt cttatcggaa taaaccaggt tgcccttcag cgacacctgc ttgacgggca
    12241 gatgattcga attgtaaaac caaaccagcc cggacgcagc aagcagcatc gccatcatga
    12301 caagcagcca gcgcgtcagc cgttccatcg cttcggcatt atcccacatg tgcggtcttc
    12361 aaaatttcaa tacataaatc ggcaaaaccc acgcccgtaa cggcagcgga tttcggtact
    12421 aaactatggc cggtcatacc gggcagggtg ttgatttcca acagatagag tttgccgtcg
    12481 gtatctttga ggaaatcgac gcgcacgcag ccttccgcac cgattgcctg cgcgccgcga
    12541 accgccagtt cgcgcatcag gctttcttcg gcttcggtca gatcttccga aggacattga
    12601 taaatggtgt cgttgcggtt gtacttggct tcgtagtcgt aaaactcggt cgcggggatg
    12661 atgtgtatgc cgggcaggcc tttgccgttc aacacagggc aggaatattc gccgccgccg
    12721 ataaaccgtt cggcaatgat ttcgccctga aggtgtttca attcttcgta aacgcttttc
    12781 agacggcctt ttcctttgac ttttaccacg cctacgctgc tgccttcggc cgccggtttc
    12841 acaaacatcg gcaggcccaa tttttcttcg acggcatcga aatcagtgtc gtcgtgcagg
    12901 acggcgaact cgggaacggg cagtcccaat gcctgccaaa tcagtttgca gcggtatttg
    12961 tccatgccga tggcggatgc ggcgacaccg ctgccggtat agggaatgcc caacagttcc
    13021 aatgcaccct gaaccgcccc gtcttcgccg taagtaccgt gaaggatgtt gaatgccgtc
    13081 tgaaaacctt gtgccttcaa ttcagacaat ggggtttctt taggatcgaa ggcatatgcg
    13141 tctatgcctt tgctttttaa agcattcaaa atggcggtgc cgctgtccag cgagatttct
    13201 cgttcgctgg aaaaaccgcc catcaatacg gccactttgc caaaattctg cattgttttt
    13261 gttctttcct gattgcttta tgcttgttgc cagaggtcgt ctgaaacctg atttgcggtt
    13321 tcagacgacc tttatatgat gttccgtctg tcaggcgggt gtgcctcaaa tctgtttcga
    13381 caattccagc agcgcggcgg ggacgcggtt gatgcttccc gcacccatat tcaacacgat
    13441 gtcgccgtcc tgcaaaacgt tcaacagcat ttcgggcaga tcggcaacgt tttcgcagta
    13501 aatcggctcg agtttgccca acacgcggat ggcgcgggca agagcgcggg aatcggcggc
    13561 ggcaatcggc tcttcaccgg cggcataaac ttcggtcagc accagcgcgt caacggtatt
    13621 gaggactttg gtaaagtctt caaacaaatc gcgcgtgcgg gtatagcggt gcggctggaa
    13681 ggcgagtacc aaacgttttt ccggatacgc gccgcgtgcg gcggcaaggg tcgccgccat
    13741 ttcgacgggg tggtgtccgt agtcgtccac caagagcgcg gtcccgccgt ttggcaactt
    13801 gatgtcgccg tatttttgga agcggcggcc gacaccttca aagccgagca agcctttttg
    13861 gatcgcttca accgatgcgc cgacttccag cgccacgccg atggctgcca atgcgttcag
    13921 cacgttgtgt ctgccgggca tattcagcac gacttcaaac gacccctgct catgtccttt
    13981 catttgaaca tggacggtga atttcatttg cgcgccgacg ttttcgatgt cggtggcgta
    14041 gatgtcggcg gtatcgtcca aaccgtaagt agcataaggt ttgctcactt tgggcaaaat
    14101 cgcgcggacg tgttcgctgt caatacacaa aaaggctttg ccgtagaagg gcatacggtg
    14161 gatgaaatcg ataaacgcct gatgcagttt ttcgacgctg tgcccgtagg tatccatatg
    14221 gtcttcgtcg atattggtaa cgacggacat aatcggtgtc aggtgcagaa aggatgcatc
    14281 cgactcgtcg gcttcggcaa cgatgtattc gcctttgccc aagcgggcgt tggtgcctgc
    14341 ggcgttgagt ttgccgccga taacgaaagt cgggtcaagt cctgccgcgc cgaggatgga
    14401 ggcggtcaga ctggtggtcg tggttttgcc gtgcgtgccg gcaatggcga tgccgtcacg
    14461 gaagcgcatc aactccgcca acatcagggc gcgcggaata acgggaattt gctgctccaa
    14521 cgcagcgaca acttcgggat tttctttttt gacggcggta gaggtaacga cgacattcgc
    14581 accgttaacg tgttctgcgg tatggccggg ataaacttga atgcccaggc tgcccaaatg
    14641 ctcggtagcg gcatttcgcg cctgatccga accggaaact ttaaaaccca aattgtgcaa
    14701 gacttcggcg atgccgctca tgccgacgcc gccgataccg acaaaatgga tgttggtcac
    14761 tcgatttttc atcataatgt tgcgttccgg tggatttcga tgcgtaaagg cgttatttta
    14821 aagggctgac cgtttgcgcg ccatagtttt ctgacaaata tatagcggat tgaaataaaa
    14881 aacatccatg ccgtctgaac ggcttttcag acggcatggt tcggcagttt acgccgcaca
    14941 cgcaatcgcg gcttcggcaa cgtcgtccgc gctgtgcggc aatgccaaag tacgggcgtt
    15001 ttctgcccat ttgagacatt tttcacggtt caggctgccg aggatttcgg cgagtttttc
    15061 cgccgtcaac tgggtttgcg gcaacagcaa tcccgcctcc gcctgcacca taaaacgcgc
    15121 gttggcggtt tgatggtcat caacggcgtg aggatacggc actaacaacg cacccaatcc
    15181 cgccgccgtc aactcggcaa tcgtcagcgc gccggcacgg caaatcacca aatcggcatc
    15241 gcggtaggcg gacaccatat cggtaataaa ttccacgcat tcggctttca cgcccagcgc
    15301 gtcgtaatcc gcctgcaagc tgcccagctt gccccgtccc gattggtggt acatctgcgg
    15361 acgcgcattg tcgggcagca aagccaatgc ctgcggtacg gttttgttca aaacgtccgc
    15421 gcccaaactg ccgccgacca ccaaaatttt cagacggcct tcacgccctt ggaagcgttc
    15481 ggcaggcacg ggcaggttgc taatatcggc gcggacgggg ttgccgacca agccgccttc
    15541 gtggctgaac gctttcggaa aagcgtacaa cacccgcttc gcccagcgcg acaggtggcg
    15601 gttggacaaa cctgccacgg cgttttgctc gtgaatcaca atcggcacgc ctaatagctt
    15661 cgccgccaaa ccgccgggga aggtaacgaa gccgccgaag ccgatgacgc actcgacacg
    15721 gtgtttgcgg ataatccgct gcgcttcgcg gacggtttga tacaaagtaa acggcagcat
    15781 cagcttgcgt ttgatgccgt tgccgcgcac gcctttaatc gccagcgttt ccaagcgtat
    15841 gccgtattgc ggcacgatac gctcttccat cgaatccttg ctgcccagcc aaatcacatg
    15901 atggccgcgc gcgcgcaacg aatccgccac cgccagcgcg gggaaaatat gtccgcccgt
    15961 tccgcccgcc atcagcataa aggttttacc gcccatgatt tactccaccc ggtaaccgcg
    16021 cattttccgg cggttttcat aatctatacg caacagcagc atcatgctga tcagcatgaa
    16081 aaagactgac gaaccgccat aggacatcaa cggcagcgtc agacctttgg tcggcaaagc
    16141 accgatgttc acaccgatat tgaagaaact ttggataccg atccaaatgc cgatacccga
    16201 agcgatatag gcgttgaaag tcaaacccaa atcgcgcgac tgcttaccga tggaaaacgc
    16261 ccgcaccacc agccagccgt aacagaatat cagcacgcac atcccgaaga agccgaattc
    16321 ttcggcgatg atggcaaaaa taaaatcggt atgcgcttcc ggcagaaagc cgcgtttgct
    16381 caaactcgca cccaaaccca taccgaacca ctctccgcgc ccgattgcca tcagagagtg
    16441 ggtaagctgg tagccggcac cctgcgggtc tttccacggg tccaaaaatg ccactacccg
    16501 ctgcacacgg tagggagcgg cggtaatcat cagcaccatc ccgcccaaga cgctgcctac
    16561 caggacgaaa aaatatttcc acggcaatcc tgccaaaaac agcattccaa cggcaatgac
    16621 ggtaatgacg acaaacgaac cgaaatccgg ctgtaccatt atcagcacca aaccgaacgc
    16681 caccagcata atcggcagga tgatcgcccg gaaacggccg tacatttcta atgtttcacg
    16741 acgtgcctgc ggattggtgg cggacatgat cagattggcc gtcccccgcc aaatcgactg
    16801 ccaacccaaa ctttccatgc tgcgcaacac ttcttcacgg cgcgtgaaca ggcttgccaa
    16861 ataaaggatg accgccagct tgaacagctc ggtcggctgg aaattcaacg gacccaaagg
    16921 tatccaacgg gtcgcgccat tgatttcgcg cccggcaatc aatacgacta ccagcaacag
    16981 gccggatagg gcaaaaatcc acggcacaag ccgccgccat gtcctcatcc tgcaaagaaa
    17041 ccataaaaaa ccgctcgcta tcaagccggc aacgacgaac cccgcctgtc tggtcaaata
    17101 gaaaaactga tcgccgcctt ccttcgatgc caaatacaca gaagccgaat aaatcatcag
    17161 caggctgaac gccgtcatca gcaccaccat ccacaaaagc ggcgcgtcga atttcctgcc
    17221 gtcgcgcaca atcggtctgt cgagcagcag agtgtggaca ccgtcgccca cttttaccaa
    17281 tacttccgaa atcttcaaaa aaaccacctg ccagtctgtt tgcacctgcc gcaaagggca
    17341 aaaatttcag acggcagaca atgccgtccg aacatacgat acatcccaaa tcggtattct
    17401 aaatctttac ttgccgccca acaatgacgg cgtttgcatt tcagacggca tcacaaagcc
    17461 ttaaacgctt cgataaacac ttccgaacgg tgcgcgtagc ctttaaacat atcaaaactc
    17521 gcacaagccg ggctgagcag cacgatatcg cacgcttcgg cttgggcata tgccgtctga
    17581 acggcttctt ccaaagtggc gcagtcggtc atattcagac cgcagccgtc caaatcgcgg
    17641 cggatttgcg gcgcatcaac gccgatcagg aacacgcctt ttgccttgcc ggcaagcgcg
    17701 tcgcgcagag gcgtgaagtc ctgccctttg cccatgccgc ctaaaatcac gaagagcgga
    17761 ttttgcaaac cggcaatcgc ggcggcggtc gcgccgacat tcgtgccttt gctgtcatca
    17821 atgaatacca cgccgttttt cttgccgatt ttttccacgc ggtgcggcag gccttggaag
    17881 gttttgacgt gttcaagcaa tgcttcgcgc ggcaaaccga cggcttcgca caaagccacg
    17941 gcagccatga cgttggcggc gttgtgcaag ccttgcaacg gaatgtcttt cgtggcaatc
    18001 aaatcttcat tgccttgttt caggcggcct gtctcacgtt ccaaccaaaa atcggcttcg
    18061 tgttccaacg aaaaccattt tacctcgcgc ccggcacgtt tcatggcacg gcagaacgca
    18121 tcgtccgcat tcaaaacctg cacaccgtcg ccacggaaaa tcttggcttt ggtatgcgcg
    18181 tagtcgagca agtcgtcgta gcggtcgaga tggtcttcgg aaatattcag taccgtcgcc
    18241 gccgtcgggc gcaggctttc ggtgttttcc agttggaagc tggaaagctc caacacccac
    18301 acgtccgcct ttttgccttc gcgctgccat tccgcctcca aaaccggcgt gccgatattg
    18361 cccgcgataa cggtatccag cccgcacttg atacagagat agccgaccag gctcgttacc
    18421 gtggttttgc cgttgctgcc ggtaatcgca attaccttgt cgccccggcg gttcacaatg
    18481 tccgccagca attcgatgtc gcccaacacg cgtccgccgt tttgcttgaa cgcctcaata
    18541 tccggctgac gctcgctgat gccggggctg agtgccaaaa tatcgaaacc gttgtccagc
    18601 gcatctttca gacggcctgt gtaaaacacc agcccgtcaa acatcttacc gatttgcgac
    18661 acgcgctcgg gcttcaactc cgcatcataa gcggccacct ccgcgccgtt tttgcgcaga
    18721 taggcaatca tggaaatacc cgtaccgccg agtccggcga cgaggatttt tttgttttca
    18781 agcgtcattt tggttttgtc ctaaaacaaa tcatattggg caggtggggg gatgtccgcc
    18841 cctgcccaag ccgctttcag acggcatcgc gggctgttca ataacctgcc ttcaggcgtt
    18901 ggtaaatgtc gcagccgtct tggtctccgt tttgacaagc cttaccaaac cattcttgag
    18961 cgagcacaag gtcttggcgc acgccgcgtc ctttgtaata cattgcgccc aaattgtatt
    19021 gggctgctgc atttccctgt gctgccgcct gcaagttttc ccgaaaatcc gatacgtcat
    19081 ccgcccacac cgcttggttc aagcccaagg caatcagggc ggcggcaagc catttgactg
    19141 tctgtttcat ggttttactt ctgttttagt ataagccgcc gttaacgata gggctgggcg
    19201 gattgtcgcc gcaggtttat tgcgcgttca aatgccgtct gaaaggtgtt cagacggcat
    19261 aggttcagcg gattttgagg gtactcaaac cgatcaacac caagacgatg gtaataatcc
    19321 aaaagcggac gacgacttgg gtttctttcc agcctttttg ttcgtagtgg tgatggatgg
    19381 gcgccatcag gaagatgcgt tttttggttt tcttatacca gccaacctga agcataacgg
    19441 atacggcttc tacgacaaat aatccgccca taatgacgag gacaaactct tggcggacga
    19501 tgacggcgac ggtaccgagc gcggcaccca atgccaatgc accgacatcg cccataaaga
    19561 cttgcgcggg ataggcgtta aaccacaaga aaccgaggca cgcgccgcac atggcggtac
    19621 agaaaatcac cacttcgttt gcgccggcaa cgtaaggtaa ttgcaggtat tgggcaaatt
    19681 gtgagtggcc gctggcatag gcgaagatgg cgaggccggc ggcaacgagg acgacgggga
    19741 aggtcgcaag gccgtccaag ccgtcggtga ggttgacggc attggatgta ccgacgatgg
    19801 tcaggtaaga caacaccaaa aagccgacca cgcccagcgg cagggcgatt tgtttgaaga
    19861 acgggacaat caaaatattg ttggcggaat tggcggcaag gtaaaacaat gccaaactgg
    19921 cgataatggc aacgcttgac tgccacacca ttttgaattt ggcggacacg ccgttggggt
    19981 ctttatagac gactttgcgc cagtcgtcgt aaaaaccgag tgcgcccgtg gcgagcaata
    20041 cgcccaagag aatccagata tacgggtttg cccagttgcc ccacaacagg gtggacacgg
    20101 taatggcggt cagaatcagc gaaccgccca tcgtcggcgt gccgtttttg acgaggtggg
    20161 tttgcggacc gtcggtacgc actgcctgcc cgcatttgag cgcggtcagc ctgcgtatcg
    20221 tccacgggcc gaacatcagg gaaaacgcta aggcggtcaa cgccgccatg acggcgcgga
    20281 atgtggtgta ttgaaaaata ttcagaccgg ttaaccagtt gctgaaatgt gcgagccata
    20341 aaaacatggg gcttcctttt tttgttttgt
//
LOCUS       DAWFAN010000020        19797 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWFAN010000020 DAWFAN010000000
VERSION     DAWFAN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.020"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(209..284)
                     /locus_tag="WGO65_000488"
     tRNA            complement(209..284)
                     /locus_tag="WGO65_000488"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Asn,seq:gtt)
     gene            complement(377..736)
                     /locus_tag="WGO65_000489"
     CDS             complement(377..736)
                     /locus_tag="WGO65_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ0326826.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(759..1403)
                     /locus_tag="WGO65_000490"
     CDS             complement(759..1403)
                     /locus_tag="WGO65_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ0326827.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1405..1941)
                     /locus_tag="WGO65_000491"
     CDS             complement(1405..1941)
                     /locus_tag="WGO65_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ0326828.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1953..3050)
                     /locus_tag="WGO65_000492"
     CDS             complement(1953..3050)
                     /locus_tag="WGO65_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0326829.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3053..4489)
                     /locus_tag="WGO65_000493"
     CDS             complement(3053..4489)
                     /locus_tag="WGO65_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ0326830.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="WGO65_000494"
     CDS             complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="WGO65_000494"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ0326831.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5479..6126)
                     /locus_tag="WGO65_000495"
     CDS             complement(5479..6126)
                     /locus_tag="WGO65_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ0326832.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="WGO65_000496"
     CDS             complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="WGO65_000496"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ0326833.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            7501..8577
                     /locus_tag="WGO65_000497"
     CDS             7501..8577
                     /locus_tag="WGO65_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ0326834.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8784..10250)
                     /locus_tag="WGO65_000498"
     CDS             complement(8784..10250)
                     /locus_tag="WGO65_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ0326835.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10448..11371)
                     /gene="speB"
                     /locus_tag="WGO65_000499"
     CDS             complement(10448..11371)
                     /gene="speB"
                     /locus_tag="WGO65_000499"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ0326836.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11470..13362)
                     /gene="speA"
                     /locus_tag="WGO65_000500"
     CDS             complement(11470..13362)
                     /gene="speA"
                     /locus_tag="WGO65_000500"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ0326837.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVCPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13529..13813)
                     /locus_tag="WGO65_000501"
     CDS             complement(13529..13813)
                     /locus_tag="WGO65_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0326838.1"
                     /translation="MKKLLLAAVVSLSAATAFADDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13964..15772)
                     /gene="aspS"
                     /locus_tag="WGO65_000502"
     CDS             complement(13964..15772)
                     /gene="aspS"
                     /locus_tag="WGO65_000502"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ0326839.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGGFPRMPYAEA
                     MFYYGSDKPDMRIGLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(15830..16531)
                     /locus_tag="WGO65_000503"
     CDS             complement(15830..16531)
                     /locus_tag="WGO65_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ0326840.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16674..17822)
                     /locus_tag="WGO65_000504"
     CDS             complement(16674..17822)
                     /locus_tag="WGO65_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ0326841.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17982..18245)
                     /gene="rpsT"
                     /locus_tag="WGO65_000505"
     CDS             complement(17982..18245)
                     /gene="rpsT"
                     /locus_tag="WGO65_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ0326842.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18548..19690
                     /locus_tag="WGO65_000506"
     CDS             18548..19690
                     /locus_tag="WGO65_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0326843.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5096 a         5112 c         4602 g         4987 t
ORIGIN      
        1 gcgagccaat gccgtactgg tttttgttaa tccactataa aatcgagttt atcctagttg
       61 tccaggacag cccctatttt ttcataacca tcacgaaagg aattttgcaa agattttatt
      121 ccatctcaaa acaatcatct caaaaatgcg tttctgaccg ccggtaaaac aaaaccctct
      181 aagaaaatac ttagagggtt ttgaaatttg gctccccgac ctgggctcga accagggacc
      241 tgcggattaa cagtccgtcg ctctaccgac tgagctatcg gggaataagc gcgaataata
      301 aaacagaaat accgaacgtg tcaatataaa ttgacaatcg attgcacttt atctgcaagc
      361 ccgcaatctt tatatgttat atggcttccg caatatgcct tgccgcacgt ttggcatcca
      421 ataggattaa accaagtctg ccggtttctt ttgccaccag caccaacacg gcatctttac
      481 ccgcctgact taaaaggata tagcctgatt ttcctttaat catcacttgt tccaattccc
      541 cgcaggcgag ttcctgcacc gagcgactcc ccaaagcaag caaagtggca gaaatcgccc
      601 ctaccctgtc cgaattcaaa tgtgaaggaa gcattgtcgc catcggcaat ccgtcggttg
      661 agataacggc agacgcgata atatccgtag atgtattgtt taaatcttca aggattgaaa
      721 tcaataattg ctgcataacc cctcctctct cccaagtttt acacgcggtt gctgtaacgg
      781 cggtataaaa tccttaccaa agtaacaaat gcctctttgc ccaaatcggg aatgccgccg
      841 ataaccaaaa taaatttggt tgaaccgata tacaatggga aaaatgtcaa ttcgctctga
      901 ccggaaggat cgcaaacgcc ccaagcgtta ttgttgatat acaggttgtt cttaatcagc
      961 agccggtatt tcttttccat ctgtgcgact tctgccgcca acaaccccaa ctcttccgcc
     1021 gcctcatgat ggaaattggc gttggcaaga tacagaccgt tccgatcgac caataacgcc
     1081 ttaccgctgc cggacaattg ttccatcagc aacggcaatt gctcgtccga caaattgatg
     1141 ccgtctgaat gaccgttttc atcgccatag aggaattcga gtttttgcaa acggtacaac
     1201 aggttcaaag cggtatcgat gtcggcggtg tccgcccaag taagcagctt ctcactgctg
     1261 accatttcgt ccgcatccgc tttcaacagg ctgtgcaaca aagttttacc ggcactgggg
     1321 gcatcgctgg atacggcata aaatgcaccg gcaggagtca ggcggggata taaatttgct
     1381 tgtagtgaaa gtgttgattc catattaaac ctccagtccc ggatcaatag aaaataacat
     1441 tgcgctaacc aattgtttta cgtcatcttc cttacgggca tcaatttcaa aaaccggaac
     1501 attaagatta tgttttgcaa gatatttgtg atacacgtcg ataccgggct gagagcgtat
     1561 atccatcttg gtaataccga caacgacggg tgccttctcc agcagccctc gaaacgaatg
     1621 taaaaagaat tccaaatctt tcaacggatt ggttcgggca ttatctaaaa gcaagaccaa
     1681 acccatactg ccttggctta agatttccca cataaagttg aaccgttcct gaccgggcgt
     1741 accatataaa tggactttgg tatcctcatc caagctgatg gccccgtagt ccatcgccac
     1801 tgtcgtattc cttttcctat ccaaagtcat atcggatgcg gaagcatcgg tctgaacgag
     1861 tgcttcgtcc gaaatagccg caatggcagt ggttttccct acgccgacag gtcctgtgaa
     1921 aataatttta ttttctctca tctctccgcc tcttagctgc ccaacagttt tttcatcagc
     1981 ctttgcagaa ggccgcgcga ctgggattgg gatggtgttg tgattttttc cgcttttttc
     2041 atcatttcac tatcagaggc agaatcggct ccgatattta ttttatccgc catatcggag
     2101 tatgcctgtt gtgaaaccgt ttttaaatct accgacaaaa acccggttgt ataggttgcc
     2161 gcaagataat tcagaatatc attgaggttt aaaggcatca ctttatacaa cacgttaagg
     2221 ttgacggatg ccttggtcag aaatgccgac aagcgtatcg accctggcac atttgccaac
     2281 cgggtcaggt ttggccaaga tttcaacgta aacggagtat cgggagaaat cggataaatc
     2341 aacctgccct gcgccgtcca aatggaaaac tgccacatac aggacataat gcctacttta
     2401 gccttttcgc gccattgcgg gttatcggga acagtcttgc agctgacctg caaattttcg
     2461 tctttgcaca attcttcgag tttttgcaca ctttctgtca gcaaaacccg ttgtatcgag
     2521 gggaaaacaa taaggaccgg cttatttcca tgcaagatag cgatgtcctg cctgttcttt
     2581 tccgcaaacc gcaacgcccc caataatcct ttattcgggt taaactggcg tatcgtaacc
     2641 gtacgctgca cattcccgtt atttttcgcc gaccggtctg cgggtgcaat aaacgatttc
     2701 ccataaacat tctcgccctg caacaatttg cggagcatag gaaacaatgt ttcaaaccga
     2761 atcggtttgg gcaggtaggg aacttcagaa tcgggaactt tctccgaaca gacggcgacg
     2821 ggtatatcct tataacgctc ggcaagctct ttccaaagtt caaaaccgcc ctcggcatcg
     2881 gtatccgcca aaaccaaatc gggcacggca ctgccgtctg aaggggatac tgtttcataa
     2941 cgggtggtat tgtgcatttt gaatgccatt ttgaaaacgg attcctgctg cgccgtcatc
     3001 cccgccaaca ttacgcgtac tgttttaatt ttcggcagtt gaacttccat ttttattttc
     3061 cgtaccgttt tattttttta atattttgat tcatacgctg cagcagccgg ctcatcagca
     3121 taacgacctc ttcaggaagc ctgtccgcac gttccctcaa tacccttaaa aactgcccca
     3181 acctatccca atcttcagta cgttcataaa tatcgatcaa cgtaatataa agctgggact
     3241 cgtcgggata tttcaatacc gcctgctcca acacatccat tgccgcttca atctgaccat
     3301 acatcagcaa cgactctact tccttaaccg catcgtctgc cggagacgaa ccggtgttga
     3361 tcaacgaaga atcttgaagc accaaatccc gatgttgcga tttgaatttc tgtatatttt
     3421 tcggcagata cccgtgcccc ataccgatgt ctttgatttg ccggtcgttc ggcccttttt
     3481 ccaaatcatc gaaaacttca tggtaaccca agctgtaccc ccaccccagc atccgctctt
     3541 taacctgcct gccgtagttg cccaacgttt ggtaaagttt ccacaaatgt ccggcaaaac
     3601 ggtctatgtc cgcgtgttgg taatcgagtt tcaacgcatc gataatcagg tttgcaggtt
     3661 tttcggaagt ttggatggca cggtcgtatt gtttcgatgc cgtttcatag ctgactttgt
     3721 ctttaaggat tttcgcacct tgatcggcac ggaccaaacc ggcaatcgca ccgatttcct
     3781 cttgactgat tccggacacg tcttttttgc cccgcacaat cgggatgcgc ttgatttctt
     3841 cggcttcata agccttaccg ccggcatccg gtgggggcga tgcggaagct gccgtcgcag
     3901 aaccgccttc cgcacgtttt tcaatctcct gagtacccca tcccaaaccc tcttctgcca
     3961 agacgcggat acgcaaatgg tttgaatcgc gctgtaatgc ctgttcgata tattttgcca
     4021 aaagttcgga aggaatcagt ttgccgtatt tttgcagatt gtctgccaaa acatcgacat
     4081 cccccacttc gagattgata tcgagcagct cgcggataag gttttcaggt ttcgcttcac
     4141 catccggaat gccgtccaga taggcagcca aagattcggc agccttgccc tgataaccga
     4201 attgcttata aacctgatac tccgtaagcg gatcgacttc ttgcgcggat acggcggcgg
     4261 atgcggtctc ggcactttcg ttccaagacc agtcgggttg cgtgccgtct gaaaccgatt
     4321 gtccaatctg atcgacccag tccgaatcat tgcttccggg tgtctgcccc tgcgtattgc
     4381 cgacaccttg ggatttgcgc ttcggtgttt tcttaccctg cttcgcacgc ataaccaaaa
     4441 gcatcagcaa taccgccagt gccaaaccga taattaatga gttttccaaa ggatactccc
     4501 gataccgtaa cgagcggata gctgaccgcc tgcaacggcc attgtccgct tttattatta
     4561 acgatttact gaaatagact tgtaagtttt aaatcatacc ataatttaac gtttaacaat
     4621 atgccttctg cacaagcctc gccatattac cttttaccca catcagtatc aatacccgat
     4681 ataaaaataa ctttgcccat aagctgcctt attgcctgcc cgccgcagag tagcgcgcga
     4741 taaaaaataa cggatgataa gtcaggtgca ctttgcccga cggcatactg aacgccgaca
     4801 agtaatcgcc aataaatcca ttgagatttt ttcttgtcca atttttttgg ttcgtggctg
     4861 ttacgcctgt atatttaagg tgtttgagta catctaacgg cgtatcaaag tctagtatta
     4921 ccttaaaatc ctcacaccat aaaagctcaa aatctttggc taaccaagtc tgccattggg
     4981 ataaagtcgg gtaatttaag cctatatttg taatttggcg gacttctttt aaattgtctt
     5041 tgccaaaggt tgcaaccgcc aataatccgt ttgctttcaa gcctgttttg caatgggcga
     5101 taaaagcgtc gggttgatga aaccattgca cggcagatgc gcttgcgatt aagtcaaatt
     5161 gtcgttgaaa aggaaagttt tccgcatcgc cgcaataaaa atcaaaggat tgcggcagtt
     5221 tttcagccag ttggggctgc acatcgcaca aatcattaaa taaccaataa ttcgctgaaa
     5281 tctgtttttg cagcaaggca ctcaacattc ctgagccgca acccaattcc aacacgtttt
     5341 ccaatggcat atccggcaaa taatcttgca aatgcgccat tagattaatc gtcatttttt
     5401 gttggattaa ggcgtgccgg tcataatcgt ttaatgcttt ttggaaagcc tgccgaatgc
     5461 gcgatttatt tatggcagtc agtgattcca tagtgccgac caatgggtga atcttgaaaa
     5521 caggtaatgt ccgccgtcaa tttcccgaac ggtgcaacgc ggtgtccaat atcggtgctg
     5581 attggcaggc ataaaaattt tatcgcccga tccgaccaag gcatttgtcc agcgaatcaa
     5641 atctgcgcgc ctatcttgcc cgatcatcgc aaaaagtgcg ataagttctt gatgaatttc
     5701 gtcaaacgga cgggcgggaa attgttggta atcttcaaaa gatgctttat cgccacacat
     5761 tctgcgttca aattttaaac gggtgttttc cgtgaggttc tccaatgtgc ctttaaaaac
     5821 ggcgcaaggg ataccgaaat tatcatcgca aggcaaacct gtgccattca ctgccgttgc
     5881 ggattttaat cttattcctt gcaatgccct ctctgccgcc caaacgccca ttgaccacgc
     5941 caccaaacgg atgtgccgat aggcggaaaa atcaaaatcc aaatttaaat cttgataatc
     6001 atagcaaatc aataaatcgt gattttccgg caaaatcaaa tgatttacag catcgggcgg
     6061 cgttccccaa cctgcaaaat acaggattaa atgttcgcct tgatgattgt aaaattttgt
     6121 tttcatatca tatccttaca ggcacgccgc aaactgccgc acttcatcca ttgtcatatc
     6181 tgccgtcaaa gacaatcgta ttctggatgt accttttggt actgtcggtg gtctgatggg
     6241 caggcaataa taaccctgcc tttgcaggta ttcagctttg gcaagggtgg cttcattccc
     6301 gcctaaaata taggggacga tacaggtttg gctcggcatt atttgcgtcc gatgcgccac
     6361 ttcccgccgt aaaaatgcgc ttaactgctc aagatggctt ctttcttttg agaattgcgg
     6421 caatcgttca aaaataaaat aagtccaagc cacattaaac ggcggcaatg cggttgaaaa
     6481 aatcaatggg cgcatttgat taatcaaaca ttctttcaat acttggttgc agacggcata
     6541 cgcccccacc gaggctaagg ctttaccgaa agtgccgacc aataaatcaa tctcggcaat
     6601 caaattatcc cgttcggcaa tccccaatcc gttttgccca taaacaccga ttgcgtgggc
     6661 ttcatccaca taaagataag tattgggaaa ctgttttttt aattggacaa gctgtttcaa
     6721 atccgccaca tcgccgtcca tactgaaaac agattcggta acgataaaag tgcggtcaaa
     6781 ttttccgacg tttttttcaa gcagattttt caaatgttca taatcattat gacgataacg
     6841 gaaaaacgca caccggctca aacggatgcc gtcaatcata ctggcgtgaa caaatttatc
     6901 tgccaaaatc aaacttttcg tcgtcgtcaa agcaggcaaa ataccgagat tggcgtgata
     6961 gccgctgttg aacaataacg cgctttcccg ttggaaacgt tgtgcgacaa gctcttccaa
     7021 atcggtataa ataggaaagt tgcccgttaa taaacgcgat gaagaactgg taaaagaggg
     7081 aaaattaccg ccgtattgct gcaaaaaaga ccggcgcaag ttttcatctg atgccaaacc
     7141 caaataatca ttagacgaca tattcagcat tttgcagttt tcccgcgtaa tataccgccc
     7201 ttgatgaatc aaatccggaa tcgaacgata ttggttttgc gcgccgagtt gttcaagctg
     7261 ttgtttaaaa accttcatta tgatgtaaat attcctgagt taaagcctga accaacccag
     7321 catcaaacaa tcaagtaatg cccttttaat aaaaaccgtt ttgccgccga cctttgccac
     7381 acccctgcct tcataatcca aggcggaaat ttccgcgaca tttgtttccg ttgccattac
     7441 ccaatccgtc cgcactgtac cgaagcgcat attttctcac aaattggtac ggtttcaggt
     7501 atgatttgcc gaaaacctat cggacacaca ccatattttg atatgaataa aacaatctgc
     7561 cgcactgctg cactattgat aagcggtttt tcatacgcaa acaccgtcat tcccgatgtc
     7621 tcccccgtcg cacaaggtca acatgtcttc attaacatcc ctcagcaacg cctgttcctc
     7681 tacaccgacg gcaaactgac caaggtttat cccgttgcag taggtcgggc gatgacgcaa
     7741 accaatctag gcgaacataa aatcggtgcg aaagcctata accctgtttg gtacatcccc
     7801 aaatccatac agaaagaacg cggcgacggc gtaaaaacca tagccgccgg cccggacaac
     7861 cctttggggc cggtttttgt ccgcttaggc gaccccaaac tcggtttggg tatacacggg
     7921 accaatgcgc cggccagcgt ccccggggtt cggagtcacg gctgcgtccg catgaaatcg
     7981 cccgacgcgc tcgagtttgc caaaaccatc gccagcggct cgcccgcctc cgtcatctat
     8041 caaatggcgg gtctcaatga agatgcggat cgcaacctgt ggcttgccgc cttccgcgac
     8101 ccttacggta agaacaacct tgacatcgcc tctctgaaaa aaagtattgc gcaatgggca
     8161 aaaacacagg gtaaaaccat cgcgcccgag aaagtcgatg ctgtactcaa agaccgcacc
     8221 ggatcggccg tctgcctgac ctgcggcaaa aacggcaaga tgaagatgcc gctcaaatcg
     8281 ctggcgtgga tacagggttc ttcctcatac agccaagctg aagtcattga acaaactgag
     8341 gaaaccaact ctgccgaaat atccgagaca cgcacgcccg aagtgcctga tgtacacacg
     8401 cccgaggcac aaccgcattt aaatacccaa tccgacggca cgccgactgc ctataccgaa
     8461 ccggctgccg attcatcgcc gcaagtagaa acacctgatc aggcagcttc cgagccggtt
     8521 gatgtattat tttcaataga tgtgatacgg cagggaaatt tgcgtttagg taattgaata
     8581 accttctgat tatttaatgc ttatgtttat cagaagttga taggcggttt ggtttttgtt
     8641 tggcttttcc tacaccgccg cctgccgctt tctgcaacat tcaagcgcac aaatatgcca
     8701 tctgaaggct ttagacggca tatttcacga taatagtcgc ccaatcaaat aaacacagat
     8761 tggaaattta ttttattttt cccttaccaa taccccagaa ctttccacca aatgctgccg
     8821 ataacgaaaa aaatcagaaa attgactacg ctcatgataa aacccgcctt ccaccattct
     8881 cccattgtgg tgtagcccga accgaaaatc acaggcgaag taccggtcgc ataatgagtg
     8941 agggtcatca taatgttgga tgcggccgcc atcatcagcg cggtcggcat cgccggggca
     9001 ttcagtgaaa cggcagcagc gaaaaatgcg ccgaacatag cggtaatatg tgcagtagta
     9061 ctggcaaaca tataatgcgc atacatataa gcaagcacga ggattacgcc cgcagccgtg
     9121 ccgctaacgc ccaaaccgcc gacactttcc gccaacactc cggagaacca tttaatcagt
     9181 ccgagtttat ttaaaaatgc ggccatcata atcaatgcgc caaaccaaat aatcgtatcc
     9241 cacgcgcttt tttctttcaa aacatcgtcc caagtcaata caccggaaag caaaagcagg
     9301 cttaatccga taaatgcggt ggcggtggcg ttgatactaa aagcgtgatt gccggtaata
     9361 agggcgggaa catctgccca caacagcaac aagataccga aaatgaccgc cataatgatt
     9421 tcgtctgccg acattttacc catctccctc agacggtctt tggcaaattg aacggcattg
     9481 ggcgtttctt taatttcagg cggatacaaa aaatataaaa tcaaaggcat aacgaaaaag
     9541 gcgataacgc cgggaacagc cattgcccac gcccacgccc cccaagaaag acggaaacta
     9601 ctgcctaaat tttcggcaat caagttgacg attaaagggt tgggggcagt tgcagtaata
     9661 aacatagccg acgaaatggg attggaatga tagttgacca aagccaaata tttacccatc
     9721 ttgccttctg tgccttttgc gggattggag ccgtaactgc cggcaatcga ctgcataatc
     9781 ggatgtataa tgccgccgcc gcgcgcggta ttggaagggg taacgggagc cagcagcagt
     9841 tcggaaagag cgagactgta accgatgccc agcgtttttc ttccaaaaac ggcgataaac
     9901 aaatatccga tacgcatccc cagccctgtt ttgagcaaac cgcgcgaaat cataactgcg
     9961 atggcaatca gccaaatcaa cggattggcg aacgcactca acgcatcgct catcgccgcg
    10021 cccggtttgt cggcggttac gccggttact gcgaccaacc cgacggcaat aatcgacagc
    10081 gcgcccaacg gcatggcctt gccgataatg gcggcaatca caccgacaaa catggccagc
    10141 agcgtccaag cctgaggctt gaccccgtcg ggtacgggca gtgccaaaac cagggcgcac
    10201 aatactgcgg caatggcgag gggtatcggt ttgaaaccca atttcatcat attgacctcc
    10261 gtaaaaaaga ccgtcccgaa aaatcggaaa aataatattt aactaattgt tttataagat
    10321 atattccgat atttcaccgt ctttccgata tgcggctccg ggcaactttt gtttcagtat
    10381 ttgaattttc attagactga atacgccgtt tgaacggcac ggcgaaaacc cgggggatgc
    10441 cggacgttca gtcctttttc gcaccttgaa ggtaaagcat ttccaaggca attgtggcac
    10501 cggccaaagc ggtaatgtcg gattggtcgt aagagggggc aacttctaca acatccatac
    10561 cgacgatgtc gagatccgtc agcccacgta ggatttttaa tgccctgtcg ctgctcaagc
    10621 cgccgcatac gggcgtaccg gtcccggggg cgaacgacgg gtcgaggcag tctatgtcga
    10681 aagtcaggta aacgggcata ttgccgacgg tttctttgat tttacggacg gtctcttcaa
    10741 cactgtcttc attgactttg ggggcggaca acacagtaaa aggcaatttt ttactgtgtt
    10801 cggtgcgtat gccgatttgt acggaacggg acgggtcgat gaggccttcc ttgggggcgg
    10861 tatagaacat cgtaccgtgg tcgtattcgc tgccgttgtc gtaggtgtcg gtgtgcgcgt
    10921 caaaatgaat cagtgcgagt ttgccgaaat agcgggcgtg ggcgcgcaac aacgggaggg
    10981 taatgaaatg gtcgccgccc aaactcaaac agcgtttgcc ggaagaaagt aatttgccgg
    11041 cgtgcgcttc cattttttcg acaaaatccc tgctgtcgcc aaaagaaaaa accaagtcgc
    11101 cgcaatcaat aatgttcagg cgttcgcgca catcaaatgt ccatggaaac ctgcggtgct
    11161 cccaagcgag gttgacggag gcgcgccgga tggcttcagg accgaaacgc gcgccggaac
    11221 gccctgaaac cgccatatca taaggcacac cggtaataac ccaatcggca tgactttcat
    11281 acggcataaa attaagcggc aggcgcaaaa acccgaaatt attggaaacg agggagttgt
    11341 cggtttgtcc tgccagtgtg ctgtattgca tcgtaatgat tccttgtaat tggtttcaat
    11401 cggtcgtgat gattggtgtt tgagtaagaa aatcgggctt cagacgacat atccgatgcc
    11461 ttgatgcgtc tattcgtctt ccaaataggt ataaccatta agccccgctt cgagttcttt
    11521 taagaaagac atagcctgcg aggcaggaag gtctgaatgt tcgatttgtt cgcgatagcg
    11581 tttcatcagc tctttcggat cttgataaac gtattcgagc atatcggcaa cggtgtttcc
    11641 ttcatcgtaa tcgatgacgg taaattgtcc gtcttcccct acaacaacat cggcagtggc
    11701 agtgtcgccg aaaagattgt gcatattgcc gagtatttcc tgatatgctc ccaccataaa
    11761 aaagcctaaa agcggcggct cttcttcggg ataatcaggc ataggcatcg taccggcgat
    11821 gccgtcgccg tcgatgtagt ggtcaatcgt accgtctgaa tcgcaggtaa tgtccaacaa
    11881 cacggcgcgg cgcgcaatcg gttcattcaa accggtaatg ggacaaacag ggaaaagttg
    11941 atctatgccc caagcatcgg gcaaagattg gaagagtgag aaattgacat acagcttatc
    12001 ggcaaaacgt tcttgcaatt cgtcaataat ggttcggtga gaccggtgtt tttcattaaa
    12061 caattcgccg acttcatgac agatatttaa atacagttgc tccgcccacg cacgttgcgc
    12121 caaactcaac agcccgacat tatactgatt atgcacatcg gcaagatcaa actgcccttc
    12181 gtgtatccag ctgcgtaagg aacgtttttc ccgcgaggcg gaaatatccg tccaagtttc
    12241 ccacatactg tgcaacacac gcggcgcttc gggcgatggc gcatccagcc ggcgcggttt
    12301 gtaacgctca acgcctataa cattagcaac caaaacggcg tgatgtgcgg taatgccgcg
    12361 cccgctctcg gtgatgattg tcggatgcgg cagcccgtgt tcgagacaag cctgactgat
    12421 gccccatacg actgtggcgg catattcgtt gaggctgtaa ttaacggaac aatccgattg
    12481 tgtgcggttt ccttcgtaat ccacgccaag cccgccgcct acatcaaaac agcggatatt
    12541 tacccccagt ttgtgcaact caacataaaa ccgagccgat tcgtgtacac ctgtggcaac
    12601 atcacggatg ttcccaagct gcgagcccaa atggaaatgc aaaagctgca ggcaatccag
    12661 cctgtttttt tgtttcaaaa tatcgaccag ttgcaaaact tgggaagccg acaagccgaa
    12721 ttttgatttt tccccacccg aagactgcca ttttcccgaa ccttgggaag ccagtctggc
    12781 gcgcacaccc aaacggggct tgatgccgag tttttccgcc tcttccaata ccatttgtat
    12841 ttcggacagc ttctcaatca ccaaataaac ctgatgcccc agtttttcgc ccatcaaggc
    12901 gaaacggata tattcacggt ctttatagcc gttgcagacg attaatgttt gccgggtgcc
    12961 ggcgtgtgcc aaaaccgcca tcagttcggc tttagaacca gcttccaaac catgcggttg
    13021 tccgcttgac ataagcgatt cgatgacgcg gcggtgttgg ttgaccttga tagggtaaac
    13081 caaacaataa ccgcccttat agccgcactc ttcccgtgcc gtctgaaagg cgcggttaat
    13141 gtcgcggagg cggtgttcga ggatttgcgg aaaacaaaac aaaacaggca ggcgcgcctg
    13201 atgtttttgt tgcacggctt cagtcagttt ttgcagtgaa acagtttgat tgtgttgcga
    13261 gggattgggg cagacgatga tttcgccgga atcgtcaaca tcataataac ctatgcccca
    13321 atgattaatg ttgcacactt cacggatggt aaggataggc ataataaacc tgctccgtct
    13381 gtcgtgttga aaaggaatga ttataacaaa tcagcgtgaa atgtcatttt tttaataaga
    13441 aaagcctgcc tcatacctga tgaggaacag gcaaaatgtc gtctgaacgc ttcagacggc
    13501 attttggttc atctttccat caaaggagtc agcgatcgag ctgctctttg atgattttca
    13561 ggtcggggta agacaacacg atgtcgtatt cgcggccgtc tttataggct tccacttcca
    13621 aaacaggttt gccccaatgg tcgtcggcat cgacatcgtg aacctgataa ccgcgctgtt
    13681 ccaacatttt tacggctttt gtgcggtttt gttcaaaatg gggatcaccg taaatctgac
    13741 gctcggcaga atcgtcggca aatgcggttg cggcactcag ggaaacaacg gcagccaata
    13801 acagtttttt cattttcagt cctttcttaa tcggttgatt gaataagatg tgtttttcaa
    13861 tgctgccatt aaaacacagc aaaattaggt ttgaattagc aggaagttaa gaagcgtaaa
    13921 tgccgtctga aaaaaacaga gccgtcaaac ggctccgttt tccttatgct tctttggttt
    13981 cagccgcctt ttgacgcagg cgcaagctca attcgcgcaa ctgcttgtcg tccacgctgt
    14041 tgggcgcgtt ggtcagcagg cattgggcac gttgtgtttt cgggaaggca atcacgtcgc
    14101 ggatagattc ggcgccggtc atcagggtaa ccaaacggtc gaggccgaat gcaaggccgc
    14161 cgtgaggagg cgcaccgaat ttcaggttgt ccaagaggaa gccgaatttc tcttgttgct
    14221 cttcagggct gattttcagc gcggcaaaca ctttctcttg tacgtctgca cggtggatac
    14281 ggatagagcc gccgccgatt tcccagccgt tcaataccat gtcgtaggca cgggccaggc
    14341 agtttgcagg gtcggaaacc atcaggtctt catgaccttc tttaggcgcg gtaaacggat
    14401 ggtgtacggc aacgtagcgg tcggcttctt cgtcgtattc gaacattggg aaatcgacaa
    14461 cccacaaagg tttccattcg tctgtgaagt agccgttgtc tttgccgtgt tccaagccga
    14521 ctttgatacg caatgcgccg atggcttcgt tcacaacttt ggttttgtcc gcaccgaaga
    14581 agataatatc gccgttttgt gcgccggtac gctcgatgat ttctttcagg gcattctcag
    14641 acaggaattt tacgattgga gattgcaggc cgctgtcttc gccgtttgag aggttgccca
    14701 catcgtttac tttgatgtat gccagacctt tcgcaccgta gatgccgaca aatttagtgt
    14761 attcgtcgat ttctttgcgg ctgaatttcg caccgttcgg cacgcgcagg gcaaccacgc
    14821 ggccgccttt catatcggct gcgccacgga agactttaaa ctcttccgtc ttcatcaggt
    14881 cggtcaactc ggtaaatttc aagccgatac gcatatccgg tttgtcagaa ccgtaataga
    14941 acatcgcttc ggcgtaaggc atacgcggaa aaccgcccaa gtccacgccc agcgcgtctt
    15001 tgaagacttg tttcgccata ccttcggtga tgtccatgat ttcatcctcg tttaagaacg
    15061 aggtttccaa gtcgatttgg gtaaattcgg gctggcggtc ggcacgcagg tcttcgtcgc
    15121 ggaagcactt ggtgatttgg tagtaacggt cgaaacccgc caccatcaac agttgtttga
    15181 ataattgcgg cgattgcggt agcgcgaaaa actcgcccgg atgaacgcgg ctcggcacga
    15241 ggtagtcgcg cgcgccttca ggcgtggagc gggtcagcat cggggtttca atgtcgatga
    15301 aaccttgcgc gtccaagtag cggcgaacgc ccatagcaac ttggtaacgc aggcgcaggt
    15361 tgcgctgcat caccggacgg cgcaggtcga taacgcggtt ggtcaggcga acgttttcgc
    15421 tgatgttttc gtcgtcgatt tggaacggag gcgtagcggc agcgttcaac acttcgattt
    15481 ctttggcaag gatttcgatt ttgccggaaa tcattttgtc gttggtcgtg ccttcgggac
    15541 ggttgcgtac gcggccggta atgctcaaaa cgtattcgtt gcgggcggag tcggcagcgg
    15601 caaatgcttc gggcgtgtca gggtcgataa cgacttgaac gatgccttcg cggtcacgca
    15661 ggtcgataaa aatcacaccg ccgtggtcac gtcggcggtg tacccagcct ttgacggtaa
    15721 cggtttggtc taagtattgc tcgctgatca ggccgcagta gttggtacgc ataaaatcac
    15781 cttttattga tttaaactga aaacagaaaa tgccgtctga acggcggctt tattgttgtt
    15841 cgggcaaatc cgccttttca gacggcatag gtcctgccaa tgttttgacg ggcaggtcgt
    15901 cagggatgac catacccagc gaaatcacat atttcaacgc ttcgtccacg ctcatatcga
    15961 gttcgcgcac atcgcttttc tttaccataa tatagtaacc gccggtcggg ttgggcgtgg
    16021 tcgggacata cacggaaaga taatcgccat cctgcggcaa tgcggcctta accgcattcg
    16081 acacctgccc tgacacgaaa gcaatcgtcc aaatacccga ttggggaaac ggcacgagta
    16141 ccggcgtttt aaacgagcgg ctgctgtcgg acagcagcga ttcggatact tttttcacac
    16201 tcgagtagat ggatttgaca accggaatcc gccccaacag gctgtcccac gcggcaagaa
    16261 tctgccggcc caatacgttg gcggcaaata atccggttac aaacaatacg gcaatggcaa
    16321 caataacgcc cagccccggg atattgaagc ccaaaacata ttgcggccgc cattgcttcg
    16381 gcagcaggtt gacgagctga tcggacgcgg aaacgatata ggaaaccacc caaaccgtta
    16441 ccgcaatcgg cagccagacc aaaatgcccg taatcagata tttttttaac gccttggcag
    16501 ctttgccgcc ttcggccgca ggttccgtca tcttgcttga ttccgacaaa gtccgtacaa
    16561 accgcacatt atacgcgttt gcccggattc aaacgaaatt tttatcccgc cccgccaaac
    16621 cgccggcggc ttcagacggc acggcaactt gatatatcgt ctgaacacgc ggttcagatg
    16681 ccgtccaagt cgttgaacat caacccgata ccgataccgt tctgcttgtg gttgtagtcg
    16741 atcaggctct cgccgtaacc gtggaatccg cgtaccacgc ctttgagttt gcccttaatc
    16801 ggaaacgtgt aggcggcttc aatcgcgccg tagcccgttt tggggttgta gcgcaatacg
    16861 gaatacacat tctgcctgtc gttcaggcgg tactgcagct tcacgtcgcc ataccccata
    16921 tagtcggcaa tatcgggatt gtcgttttta tcgccgctct gatcgaacgc acgcacccac
    16981 acgcgcggaa tcaccgtcaa tttgccccat tccatgcctg ccatggcata aatcctgttc
    17041 cacgaacgcg attcgggacg gctctgtccg ttggactggt ggacaaaacc cgcaccgagc
    17101 atacgcagcc tgccgccgaa cggcaaatct gccttcacag gctgggtcag gaaaatttca
    17161 ggtttgtaat ccgtattgcg gaacggcgcg gatttcctgc cttggttgta aatctgccaa
    17221 tcggatcttt gggtgtagcc gaaccacaga tccgcgcggg ttttaaacaa atcttcggca
    17281 attttgcttt tgaacgaaac ctgcaatttg gtttccgcac gtttctgctg tccgaatttt
    17341 tcctgtacag tcgtaccgcg cgtcggcgaa cccggggcat agttgggcga attgttgtac
    17401 cagagcggca taaggtacat cggattgtgt tcgcgtacgc ccaacagccc gcgcaaatcg
    17461 tttttgtcca agtcgtacat caggctcaaa ggcgtataga tgtcggcggt ttcgcccgca
    17521 ctgtcggcag gaagcgcatc cccgcctttt tcaacaacaa tgaccgcctc gcccttatcc
    17581 aggctgctgc ggacggtttc cgtcagattg agtacggctt tcgactcctg cccttcctgc
    17641 cctgccgaag acggaagctg tgccgcaaaa atcctgtcgt aacacgccaa acgcgtaaca
    17701 ttgtccgtca aagcggcgca ttgcagcgcg gtctctccaa aagcggatgc catcggcaac
    17761 agtcctgtca aaagaatata gcgcatattc cgtgtattca tctccgcccc cattgtcggc
    17821 atattggttt tcaaacggca ttttatagcg gattcggata aaaaccgcac cctttccgcc
    17881 atttcgggat tttgcccccg caatacagaa aaccccgaaa ccgtcgggct tcagggtttt
    17941 ccgcttatcg cgtatcaacc gccttggcgg ttttgcaaaa atcaagccag tgcttttact
    18001 tttgcagaca gacggctttt gtggcgtgcc gctttgtttt tgtggaacac gcccttgtcg
    18061 gcgatgcggt cgatgacttt gacagactct tggtaaaccg cttgtgcggc agctttatcg
    18121 cctgcttcga ctgctttcaa tacttttttc actgcagtgc ggaatgcggt acgcaggcta
    18181 gcgttgtggg cgcgttgttt gacggactgg cgggcacgtt tgcgtgcttg tgcgctgttt
    18241 gccatattgg aatctcctga aagaaaaata aattctgtaa acgcgcaatt ttaaaggggg
    18301 tttacattat atgcaagcct tttctctcta tccgcccgtc ggacaccctg acagcgcaaa
    18361 aaaacggcaa aatcgtgcgg acgttttctc cctcttttga aaacaaggta attttttagg
    18421 aaaatcaggt caatatcgct ttgagtttgc acaaatttac tttacaatgc ccaaaccttg
    18481 cgccggggtg tttatggggc gcggggtttt ctttttttcc aacctctttc atcaggaatc
    18541 tgcccgtatg aaaaaatccg tattagccgt tttggccgcg ctgtctctgg cagcctgcgg
    18601 cggcagcgaa aaaaacgccg tgcaacctca agcagacgct gcgtctgctg ccaatgccga
    18661 agccgctgcc accgatactc tgaacatcta caactggtcg aactatgttg acgagagtac
    18721 ggtcgaagac tttaaaaaag ccaacaatct gaagctgact tacgacttgt acgaaaacaa
    18781 tgaaacgctg gaagccaaaa tgctgaccgg caaatccggt tacgatttgg ttgtgccggg
    18841 catcgccttc ctgccgcgcc aaatcgaggc gggcgcgtat caaaaagtca acaaagacct
    18901 gattcccaac tataaaaaca tcgatcccga actcttgaaa atgctggaaa ccgccgaccc
    18961 gggcaaccag tatgccgtcc cctatttctc cggcgtgaac acgattgcga ttacggcgaa
    19021 aggcaaagag cttttaggcg gcaagctgcc cgaaaacggc tgggatttgc tgttcaaacc
    19081 tgaatacacc cacaaactga aatcctgcgg catcgccctg tgggacaccc cgagcgaaat
    19141 gttcccgatt ttgctgaact acttgggcaa agaccccaaa ggctcgaatc ctgaagattt
    19201 gaaggcggcg gcggaagtgt tgaagtctat ccgtcctgat gtcaaacgtt tcagcccgtc
    19261 catcatcgac gagctggcgc gcggcgacat ctgcctggcg gcaggcaacg gcggcgattt
    19321 gaacttggcg aaagcacgtt ccgaagaagt gaaaaacaac gtcggcatcg aagtgctgaa
    19381 tccgaaaggc atgggcttct ggattgagtc ttggctgatt cccgccgatg cgaaaaacgt
    19441 cgccaatgcc cacaaataca tcaactacac gctcgacccc gaaatcgcgg cgaaaaacgg
    19501 catcgccgta accttcgccc ccgccagcaa accggcgcgc gaaaaaatgc ctgccgaact
    19561 ggtgaacacc cgttccatct tcccgaacga gcaggatatg aaagacggtt tcgtgatgcc
    19621 gcaaatgagt acggatgcga aaaaactgtc tgtcagcctg tggcagaaaa tcaaagtcgg
    19681 caccaactga tttgaagcat taaaaatgcc gtccgaacat cgttcggacg gcattttata
    19741 ttggattgaa atagaaatat ttatatagtg gattaacaaa aatcaggaca aggcgac
//
LOCUS       DAWFAN010000021        19574 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWFAN010000021 DAWFAN010000000
VERSION     DAWFAN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19574
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.021"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(243..845)
                     /locus_tag="WGO65_000507"
     CDS             complement(243..845)
                     /locus_tag="WGO65_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ0326844.1"
                     /translation="MTNVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQAMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(879..2150)
                     /gene="purD"
                     /locus_tag="WGO65_000508"
     CDS             complement(879..2150)
                     /gene="purD"
                     /locus_tag="WGO65_000508"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ0326845.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSATAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            2308..2625
                     /locus_tag="WGO65_000509"
     CDS             2308..2625
                     /locus_tag="WGO65_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ0326846.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLSTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            complement(2677..3288)
                     /locus_tag="WGO65_000510"
     CDS             complement(2677..3288)
                     /locus_tag="WGO65_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ0326847.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(3351..4286)
                     /locus_tag="WGO65_000511"
     CDS             complement(3351..4286)
                     /locus_tag="WGO65_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ0326848.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            complement(4297..5046)
                     /locus_tag="WGO65_000512"
     CDS             complement(4297..5046)
                     /locus_tag="WGO65_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ0326849.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            complement(5273..6241)
                     /gene="waaC"
                     /locus_tag="WGO65_000513"
     CDS             complement(5273..6241)
                     /gene="waaC"
                     /locus_tag="WGO65_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ0326850.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEVGFTDI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEVGRLKNLAQPYYAALHATSRDSKLWPMENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            complement(6279..6914)
                     /locus_tag="WGO65_000514"
     CDS             complement(6279..6914)
                     /locus_tag="WGO65_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ0326851.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGNWQVIVNEAACRGIAPDTIETAWQEMSSAGAVILKNAEEIKKYINNQ"
     gene            7028..7708
                     /gene="tsaA"
                     /locus_tag="WGO65_000515"
     CDS             7028..7708
                     /gene="tsaA"
                     /locus_tag="WGO65_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ0326852.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLASAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIIDIKPYIPFVESKPDASSGFVSGKPEELEVV
                     WLENIGAENLSESTQNLISQSIAQDPRPAYQDIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            7777..7896
                     /locus_tag="WGO65_000516"
                     /pseudo
     CDS             7777..7896
                     /locus_tag="WGO65_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7966..8970)
                     /gene="gap"
                     /locus_tag="WGO65_000517"
     CDS             complement(7966..8970)
                     /gene="gap"
                     /locus_tag="WGO65_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0326853.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQNILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            9412..12006
                     /gene="mutS"
                     /locus_tag="WGO65_000518"
     CDS             9412..12006
                     /gene="mutS"
                     /locus_tag="WGO65_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ0326854.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRHIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TTQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            complement(12129..12620)
                     /locus_tag="WGO65_000519"
     CDS             complement(12129..12620)
                     /locus_tag="WGO65_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0326855.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(12624..13019)
                     /locus_tag="WGO65_000520"
     CDS             complement(12624..13019)
                     /locus_tag="WGO65_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326856.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNTVYGAGGTKASGN"
     gene            complement(13040..14434)
                     /locus_tag="WGO65_000521"
     CDS             complement(13040..14434)
                     /locus_tag="WGO65_000521"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ0326857.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNAGNVVYQTRRFDRYKEVIAELLEKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            complement(14550..15206)
                     /locus_tag="WGO65_000522"
     CDS             complement(14550..15206)
                     /locus_tag="WGO65_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ0326858.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIDSVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            complement(15203..15556)
                     /gene="arsC"
                     /locus_tag="WGO65_000523"
     CDS             complement(15203..15556)
                     /gene="arsC"
                     /locus_tag="WGO65_000523"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ0326859.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIAEHPALLERPIVLANG
                     KAAVGRPLENIEAIL"
     gene            15680..16162
                     /locus_tag="WGO65_000524"
     CDS             15680..16162
                     /locus_tag="WGO65_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0326860.1"
                     /translation="MKYLNLAAIALAATFAAHTASADELAGWKDGTAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            16356..17006
                     /gene="ftsE"
                     /locus_tag="WGO65_000525"
     CDS             16356..17006
                     /gene="ftsE"
                     /locus_tag="WGO65_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ0326861.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            17003..17920
                     /gene="ftsX"
                     /locus_tag="WGO65_000526"
     CDS             17003..17920
                     /gene="ftsX"
                     /locus_tag="WGO65_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ0326862.1"
                     /translation="MSIIHYASLHIESARTALKELLRQPFGTLLTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRIANVRFIGKEDG
                     LAELQSNLDQNLVSMLDGNPLPDAFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSTVRPLVDAIFKPYGLNIGWRFFYPEEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            18011..18259
                     /locus_tag="WGO65_000527"
     CDS             18011..18259
                     /locus_tag="WGO65_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0326863.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(18326..19504)
                     /locus_tag="WGO65_000528"
     CDS             complement(18326..19504)
                     /locus_tag="WGO65_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ0326864.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASIASV
                     KYCVDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
BASE COUNT         4924 a         5482 c         4422 g         4746 t
ORIGIN      
        1 taatcatctt cggcgttttc atttttatgg attaaaacaa cacgggaaaa atctgttttc
       61 agatgcttgc ccgcttgatt gttcggatta ttgtccggaa cgacaaaacc gtcctcaaaa
      121 ttaaagcaga cgttgcgtcc ttctaccttt atctctgtgc aataacaatc atgtagagaa
      181 atgctatccg aaaaattttt tctttgtgta tgcaaaaaaa gttttcattc aagtacccat
      241 atctaacgca aacgtttacc tgtttccccg tcaataatct gactcggcga tttctgcctg
      301 ccgattcgcc caccaacaat ccacacatcg cgtccgaatt gccttctgac ttccctctcc
      361 gtccgacacg cgcgtttgcc tgcgcggttg cacgaagtcg agaccaaagg cgtttgcaaa
      421 gcctgacaca agcggcgcgc acctacatgg gcgggaaccc tgaccgccaa cttgctgcgc
      481 tgtttcccgc gtaactcagg taagacacag gatttggcag ataataggaa cgttttaggg
      541 gcgggccatt cttttctaag catagcctgc agattttcag acggcatttg aagtaaaggc
      601 tgcaattgct caaattgatt cccgatgaca atcataccct tgtgttgcgg tctttttttc
      661 aaatgcgcca acttaccgag tgctttggct aatgtcggaa gacaccccaa gccataacaa
      721 gattcggtcg gataagcgac caaaccacct tttttcaaat aaacgcttaa cttacgttgc
      781 gctgatgctg cgataattct cggaaataac ataatataaa ataccgtctg aagcacatta
      841 gtcatacttg gcttcagacg gcatcatcct ctttctaatt aacggttaat cgctttatcg
      901 gcaatgtctt tacggtattg catcccgtcg aaactgattt tttccaacgc gccatatgcc
      961 tttgctttcg cttgcgccac attgtcgccc aatcccacaa cacacaatac gcgtccgccg
     1021 ttggtcaata cgtcaccttt ctcgtttgcc gttgtacctg catggaaaac tttgcctact
     1081 tggttggcag catccagacc ggaaataaca tcgccttttt tgggcgtttc ggggtaattt
     1141 tgcgctgcca gtaccacgcc cacggcagtt tgcgggctcc attccgcggt tgcgctatcg
     1201 agtttgccgt ctattgccgc ttcaaccaaa tccgataagt cgctgttcag tcggctcata
     1261 atcggctggg tttcaggatc gccgaaacgg cagttaaact caatcgtata gggtgcaccg
     1321 ctttgatcaa tcatcaaacc tgcgtacagg aaaccggtga actcatgccc ctccgctttc
     1381 atccctgcta cggtcggcaa aataatttca ttcatcgcgc gttcgtacac aacaggcgtt
     1441 accacaggcg cagggctgta cgcacccata ccgcccgtat tcagaccttt gtcgccgtct
     1501 aaaagacgct tgtggtcttg gctagttgcc ataggcagta cattattgcc atcaaccatg
     1561 acgataaaac tcgcttcttc gccttgcagg aaatcttcaa tcacaacacg cgcgccggca
     1621 ttgcccattt tgttgtccag cagcatatca tcaatcgcag catgcgcttc atccaaagtc
     1681 atcgccacaa tcacgccttt acctgccgcc aaaccatcgg ctttgataac gataggcgca
     1741 cctttctgat tgacgtaatc atgtgcggca tcggcgtttt caaaggtttg atattgcgcg
     1801 gtcggaatgt tgtatttcgc cataaatgct ttggcgaaat ctttggaact ttccaactgc
     1861 gccgcatatt gtgtcggacc gaatattttt agtcctgcgg cacggaaatc atccacaaca
     1921 cctgccgcca aaggcgcttc agggccgacg acggtaaaaa caatattttc tttacgacag
     1981 aattcaatca aatcctgatg cgcagtcaag tcgatgtttt gcaacttggg ttcaatcgct
     2041 gtaccggcat taccaggcgc aacaaatact gtttccactt taggcgactg cgccaatttc
     2101 caagccagcg cgtgttcgcg accgccatta ccgataacca gcagtttcat gccatctcct
     2161 tgacaaatat gtacttttaa cgaaaactcg atacaaaggg acttttatcc catctgaaga
     2221 aattttagta gaatcaaaca aaagaccgct tcattccact ctgcaaccta ttcaacttat
     2281 ccataaatta aaaaaggaca agcaaccatg caaaaacgta ttgatgaaat ccaaagcaaa
     2341 taccgcgaat ggtgtcattt actaccgcaa ctggaagaag acatccgccg ttggaaatat
     2401 gtcgtcactt taattcgcga catggacaat ttctataccc acgagtatca ggcgtgtcat
     2461 caggctattg aagacggggt agaactggat ttgagtacgg aaggcgaata cagcattatg
     2521 agtgaagatg cactatggaa cgcgctgggc gaattccatc aattggcttg gttatatttg
     2581 cgctccagcg tcgatgcctt agacaaatat acacaagaag attagtcagc gaagaggtcg
     2641 tctgaaatac catcacaaag catttcagac gacctttcat tcaaaaggct tttccgtatt
     2701 tacttcaatc tgccgagtat tcttccaagc cgcaacacag gcctcataat ttaccaacga
     2761 caaactgacc gtcaatcggc aatccaactg caaatcccgc tccaatatat ccgcctgata
     2821 ttgtttggca atgcgtatcg cttcattcaa aaacggatat tcacatttca gccaaacagt
     2881 tttttcaata ttcttttcaa ctacttctgc aactgccaac gcttgagccg tcgcctcttt
     2941 gtacgcatgt atcagacctg gaacacctaa caaagtacca ccgaaatagc ggacgaccac
     3001 aaccaaaacg tcggtaatac ccaccgaatc aatctgtccc aaaattggtc gtccagcact
     3061 tcctgatggc tctccatcat cgttggcacg aaattgcatg ccatccacac ccaaacgata
     3121 ggcatagcac cagtgtcgtg ctttatgatg ctcttccttt aacggatcga ggtatttttt
     3181 cacatcagcc aatgtccgaa tcggataggc aaatgcaata aaacggctgc ctttatcttt
     3241 aaactcagcc tgcgtcgagg aagtaatggt tttataagtc gtaatcatgc tgaaatgttt
     3301 tcagacgacc tcattaataa caaggtcgtc tgaacgtttc acgtgaaaca ttaatttttc
     3361 aatacttctg ttaattgtgg aacgatttca aataaatcgc caaccaatcc gtaatcggct
     3421 acattgaaaa tcggcgcatc agcatcttta ttgattgcaa caatcacctt actgtcttgc
     3481 ataccggcaa cgtgttgaat cgcacctgaa ataccgattg caaaatagag ttgcggcgca
     3541 actactttac cggtttgtcc gacttgagca tcgtttggcg catattcggc atcaaccgcc
     3601 gcacgggatg caccgattgc cgcacctaaa acatccgcca acggtgtcag cacttcattg
     3661 aatttttccg cactacccaa cgcacgacca ccggaaacaa tcacttttgc ctgagtcagt
     3721 tcaggacgat cggaatggga aagctgacgg ttaacaaaac gactcaggtt ttgggcaggg
     3781 gttgcttcaa cattaattac ctcagcatta ccaccttgcg ccgccactgc gtcaaaaacc
     3841 gtcgcacgga aggtcagcac caatttttct gaatcagctt gcacggtttc aaatgcatta
     3901 cccgcataaa tggggcgcac aaaagtcgtg ttatccacaa tttcggtcaa atcagaaatt
     3961 tgcggtacgt ctaataaggc tgctacgcga ggcaaaaggt ttttaccgaa tgtagttgcc
     4021 gttgctgcaa catagcagta atccgctgcc aatttaacaa ccagcggagc caactcttca
     4081 gccaaacctt cggcataatg ggcagcatct gcaaccaaaa ctttttccac acccgccact
     4141 tgttttgcga actctaccac agcagatgca ccgcttccgg caaccaataa atcgatttta
     4201 cccagcttag cggcagcggt aacagcatgc aaagtggtag gattcaactg tttgttgtcg
     4261 tgttcgacaa taatcaatac actcatttca gcctcctcaa atcactttgg cttcgttttt
     4321 caatttttca accaattcgg caacgcttgc tacttttacg cctgcctgac gcgccttagg
     4381 ttcggcaaat ttcaccgttt tcaaacgagg tgaaatgtcg gcaaccaaat cgtcaggagt
     4441 cagtttttcc aaaggttttt tctttgccgc cataatattg gggagtttga caaagcgcgg
     4501 ctcgttcaaa cgcaaatccg cgctgataac agcaggcagt ttcaatgcga tggtttcttc
     4561 gccgccatca atttcgcgca caatctgtac ttcaccacct tcaatgtgta cttttgaagc
     4621 aaacgtacct tgcgccgcat tcagcaaagc tgccagcatt tgcgccactt gattggcatc
     4681 atcatcaatc gcttgtttgc ccaaaaagaa aatttgcgga ttttctttgt ccgcaacggc
     4741 tttcagcaac ttagcaacgg ccagagactc cagtttagta tcggtttcaa catgaatggc
     4801 acggtcggca cccatcgcca aagctgtacg caacgtttct tcgcatttct tttcgcccaa
     4861 agaaaccgct acgatctcgc ttacttttcc ggcttctttc aaacggacgg cttcttccac
     4921 agcgatttcg tcaaacggat tcatcgacat tttgacattg ccgatatcca catccgaacc
     4981 atcggctttt acacgaactt tgacgttgta gtccactacg cgctttactg cgaccagtgc
     5041 tttcattgaa ccctcctaaa aagaacgctg ctttcaccat ccagcgaaac caaaccttct
     5101 tccctataaa accaaatccg ttttccttaa aaacgaattc attcaaaaat ctttcggata
     5161 atgcttgccg attataccat ttttaaagca tttactcaga ctagcggata tacattcctg
     5221 tatctaataa attggaaaat atcatgccgc catatcagtt ttagacgacc ctttagcctt
     5281 tatctgctgc aacacaatcc atcagcgctt gataaaccaa atctgcggtc ggaatctgcc
     5341 cgatattgcc caaatttttt gcaattggcg aaacctgaac gcctgtttta atcggatcgg
     5401 tatcggtata aatgccgacc acaggttttt ccaaggcatt tgccaaatgc agcaaaccgg
     5461 tatccacgcc gacaattccg accgcgtatt tcagcagata cgctgcctgc aataaattta
     5521 ttttgtcgca cacaatagca aacggcagtc cgtctgcaat ctgtttggca cgcgtttttt
     5581 catcttcatt tccccaaggc aggtaaatat tgcattgctg ttcttcattc aacttttgca
     5641 gcaacgaccg ccagttttcc ataggccata acttactgtc ccgactggtc gcatgcaaag
     5701 ccgcataata cggctgcgct aaatttttca gacggcctac ctcaggaaca gtcaagccaa
     5761 atacctgcgt ttcaggcatc gcatacccaa atacttgggc gaacagctca cgattgcgcc
     5821 aaacagcatt tttccctttc ggtacagcgt atgttttcgc atacgccagc gcagccagtc
     5881 cctcgcgcgc gctattttta tccaaaccat aaatagggga tttcgccatt ttagcgaaac
     5941 acgcgctttt aatcagacct tgactgtcca atacaaaatt aaatgcttcc tgccgcaaag
     6001 tctgtttcag atgacccatt tcccgccaag tttcagcctg aaagagatgt ttgcgccatt
     6061 gccgccattt catcacatgg atttttttca caaacggatg caggcgcgca atatctgtaa
     6121 atccaacttc acacaaccaa tggagttcta catcagggca ttgtcgcgcc aaatcctcaa
     6181 ttgcgggcaa agtgtgaatt aaatcgccca tactagacaa acggacaagc aaaattttca
     6241 tattttaaga agtatgtttc acgtgaaaca attttaactt attgattatt aatatatttt
     6301 ttaatttctt cagcgttttt taagatgact gcaccggcag aactcatttc ctgccatgcc
     6361 gtttcaatgg tatccggcgc aatgccccga caagccgctt cattgacgat aacctgccaa
     6421 ttaccacctt taagtaactg caaaaccgtt gttttaacac aataatccgt agctaaccca
     6481 ccgataataa ccgtatccgt attttgacaa cgcagccatt caatcagccc tgtgcttagt
     6541 ttttcctcaa tatcgtgaaa acacgcgccg taaggatgca attcaggatc aacacctttc
     6601 caaacgcaat aatcatattc tttggcagaa ggcagtccgt ccaataattc atagccgcgc
     6661 gtaccgacca tcgcatgagc cacccaagtc aaatccgcat caggcaaacc tgtcggcttc
     6721 aacatatcaa cagggttatc cacaagccat ttcgctacca tatgatgcgc atctttcgtc
     6781 atcacgcgca aatccgccaa agcggcttga gcattcaact cctcgacaat caaatgcccc
     6841 tcgttcacgg gcagttcgtc aggacacagt ggcgtaaacg ttttttgtgc atcaacatca
     6901 atggaaacaa tcatctcatt atttcaacgc gattaaaatg ccctgtatta taacaaatta
     6961 ctgcccaaaa gcggtaaaac cgattgtgat aagataaggt ttttccaaaa aacttaccca
     7021 caaccttatg acttatacca ttacccccat cggcaccgcc cgatcgccct acaaacagaa
     7081 attcggcatc gcccgccagc ccggtttagc ctctgccgca gaagtatgca tcgagctgaa
     7141 tcccgaattt accgcagaca gcgtgcgcgg gctggaagat ttcgattatg tgtggataag
     7201 ttttattttt cacggcgtat tggatgaagg ctgggcgcaa atggtgcgcc cgccacggct
     7261 cggcggcaaa caaaaaatgg gcgtgttcgc cacgcgcagc ccccaccgcc ccaaccatct
     7321 cggactctcg ctcctgaaac tcgaacgcat cgaaaccggc aaacccgtcc gcctctattg
     7381 cagcggcgca gacctgttgg acggcacacc aattatcgac ataaaaccct acatcccctt
     7441 tgtcgaatcc aaacccgatg catcatccgg cttcgtcagc ggcaaacccg aagagcttga
     7501 agttgtatgg ctggaaaaca tcggcgcgga aaatttatct gaaagcaccc aaaacctaat
     7561 cagccaaagc attgcccaag acccgcgccc cgcctatcag gatattcccg aacggattta
     7621 tgtgatgaat attgcggatt acgaagttag gtttcaaatc gaagaaaacc gtgcaaccgt
     7681 tattgatctt tccccaaccc cgctttaaat cgggcaaaaa tccggttttg ccgcatagca
     7741 gttgaacaaa cggctgttgt ttgttcgcca taagccgcaa tatcaagtta tagcggatta
     7801 aatttaaatc aggacaaggc aacgaagccg cagacagtac aaatagtacg gcaaggcaag
     7861 gtaacgccgt actggtttaa atttaatcca ctatacagat aaacaatgcc gtctgaacgc
     7921 aatgtgttca gacggcattt acttatccac aggtttgttc aagccttaga ttttgcctgc
     7981 aaagtattcc aaagtgcgga cgagttggca ggtataggac atttcgttgt cgtaccaggc
     8041 aacggttttc accagttgtt tgccgcccac ggtcatcacg cgggtttggg tcgcatcgaa
     8101 gagcgagccg tattcgatgc cgacaacgtc ggaagaaacg atttgatctt cgttgtagcc
     8161 gtaagattcg ctggcggctg ctttcatcgc ggcgttgatt tcttctttgg ttacagggcg
     8221 ttcgaggacg gaaaccaatt cggtcagcga gccggtggca acagggacgc gttgggcgga
     8281 gccgtcgagt ttgccgttca attcagggat aaccagaccg atggctttgg cggcaccggt
     8341 gctgttgggc acgatgttga gcgcggcggc gcgggcgcgg cgcaaatcgc ctttgcggtg
     8401 cggcgcgtca agggtgtttt ggtcgccggt gtaggcgtgg atggtggtca tcaggccttc
     8461 gactacgcca aattcctttt gcaggactgc cgccatcggg gcaaggcagt tggtggtgca
     8521 ggaagcggcg gaaatgacgg tttcgctgcc gtccaaaatg ttttggttca cgccatatac
     8581 gacggttttc acatcattgc cgccaggtgc ggaaatcacg actttgcgcg cgccggcacg
     8641 gatgtgggct tcggctttgg ttttgttggt aaagaagccg gtacattcga ggacgacatc
     8701 cacacccaac tcgccccaag gcaattcttc gggattcgga ttggcaaaaa ctttgatttc
     8761 tttgccgttt accacgatgg catcgtcttt taattcggca gtaccttgga aacggccttg
     8821 cgtgctgtcg tatttgaaaa ggtgcagcag catttcggca ggggtcaggt cgttgacggc
     8881 gacgacttcg atgtcgtggg ctttttcaat ttgacgcaat gcgaggcggc cgatgcggcc
     8941 gaaaccgtta atcgctactt taatgctcat attttgtctc caggttgtga aataaagtcc
     9001 gatgcctgca ttgtattctg aaataaagtt acattccact attacatcta actacttgac
     9061 gcttatttga tatagatgaa ttttgctgtt tgcacagatt tccaaaacct ttaccatcaa
     9121 tatttgaatt taaattttaa tgatgatttt gatgattgcc aacctgcttg tgcgtaagta
     9181 gcaaatatcc aatattttca ttatcttttt gtcaaataag tttgagttta agacttgctg
     9241 tataagacag ataagcgtgg atgttttttg acttaataat atttctgtgg ataactttgc
     9301 tgttttccta gttgtctcca caaccttatt gacaggctta cggtcagtct cattccgtcg
     9361 aagacaaaac cttttgctac aataccgttt tcctaatgat aaggcagccc catgtccaaa
     9421 tccgccgttt ccccaatgat gcagcaatac ctcggcatca aagcgcaaca taccgacaaa
     9481 ctggtgtttt accgtatggg cgatttttac gagatgtttt tcgacgatgc ggtagaagcg
     9541 gcaaaacttt tggatattac cctgaccacg cgcggacagg tggatggcga gccggtcaaa
     9601 atggcaggcg tgccgtttca cgccgccgaa caatatctgg cgcgcctggt caagttgggc
     9661 aaaagcgtgg cgatttgcga acaggtcggc gaagtcggcg caggcaaagg accggtggag
     9721 cgcaaagtcg tgcgcatcgt aacgcccggc acgctgaccg attccgcatt gctggaagac
     9781 aaggaaacca accgcatcgt tgccgtgtcc cccgacaaaa aatacatcgg tttggcgtgg
     9841 gcatcgctgc aaagcggcga attcaaaacc aagctgacaa ctgtggataa attggacgac
     9901 gaactggcgc gcctgcaggc ggcggaaatt ctgttgcctg acagtaaaaa cgcaccgcaa
     9961 cttcagacgg catcgggtgt tacgcgcctg aacgcgtggc agtttgccgc cgacgcgggg
    10021 gaaaaactgc tgacggaata tttcggctgc caggatttgc gcggcttcgg tttggacggc
    10081 aaagaacacg ccgttgcgat tggcgcggca ggtgcactgt tgaactatat ccgtctgacg
    10141 caaaacctga tgccgcaaca tttggacggc ctgtcgctcg aaaccgacag ccaatatatc
    10201 ggtatggatg ccgccacgcg ccgcaatctc gaaatcacgc aaaccctctc cggcaaaaaa
    10261 tcgccgaccc tgatgtccac gctcgacctt tgcgctaccc atatgggcag ccgcctcttg
    10321 gctctctggc tgcaccaccc tttacgcaac cgcgcccaca tccgagcgcg ccaagaagcc
    10381 gttgccgcgc tggaaagcca atacaaaccc ctccaatgcc gtctgaaaaa cattgcggac
    10441 attgaacgca ttgctgcccg catcgccgtc ggcaacgccc gcccgcgcga tttggcatct
    10501 ttacgcgaca gtctgttcga gttggcacaa atcgacttgt ccgcaaacgg cagcagcctg
    10561 ttggaaaccc tcaaagccgt tttcccggaa aacctatcca cagccgaaca gctccgccaa
    10621 gccattttgc ccgaaccttc cgtctggctg aaagacggca atgtcatcaa ccacggtttt
    10681 catcccgaac tggacgaatt gcgccacatt caaaaccatg gcgacgaatt tttgctggat
    10741 ttggaagcca aggaacgcga acgtaccggt ttgtccacac tcaaagtcga gttcaaccgc
    10801 gttcacggct tttacattga attgtccaaa acccaagccg aacaagcacc tgccgactac
    10861 caacgccggc aaaccctcaa aaacgccgaa cgcttcatca cgccggagtt gaaagccttt
    10921 gaagacaaag tgctgactac tcaagagcaa gccctcgcct tagaaaaaca actctttgac
    10981 ggcgtattga aaaaccttca gacggcattg ccgcagcttc aaaaagccgc caaagccgcc
    11041 gccgcgctgg acgtgttgtc cacattttca gccttggcaa aagagcggaa cttcgtccgc
    11101 cccgagtttg ccgactatcc ggttatccac atcgaaaacg gccgccatcc cgttgtcgaa
    11161 cagcaggtac gccacttcac cgccaaccac accgacctcg accacaaaca ccgcctcatg
    11221 ctgctcaccg gccccaatat gggcggcaaa tccacctata tgcgccaagt cgcgctgatt
    11281 gttttattgg cacacaccgg ctgtttcgtg cctgccgatg ccgccacaat cgggcccatc
    11341 gatcaaatct tcacccgcat cggcgcatcg gacgacctcg cctccaaccg ctccactttc
    11401 atggtcgaaa tgagcgaaac cgcctacatc ctgcatcacg ccaccgaaca aagccttgtt
    11461 ttaatggacg aagtcggacg tggtacttcc actttcgacg gcctcgccct cgcgcacgcc
    11521 gttgccgaac acctgctgca aaaaaacaaa tccttcagtc tgtttgctac ccactatttc
    11581 gagctgacct acctgcccga agcccacgcc gccgccgtca atatgcacct ttccgcgctc
    11641 gaacagggac aggacatcgt tttcctgcac caaatccaac cgggtcccgc cggtaaaagc
    11701 tacggcattg ccgtcgccaa actcgccggc ctgcctgtac gcgcattgaa atccgcccaa
    11761 aagcatttga acggactgga aaaccaagcc gccgcaaacc gtccccaact ggatattttc
    11821 agtaccatgc cgtctgaaaa aggagatgaa ccgaatgtgg gcaactttgt ggacaaagca
    11881 gaggaaaaac attttgaagg tatattggca gcagccttgg aaaaactcga tcccgacagc
    11941 ctgaccccgc gcgaagcatt gtcagaactg taccgtctga aagatttgtg caaatccgta
    12001 tcttaatttc cgttggcgga acagcatcaa accatatgga aaaatctgtg gataaacatt
    12061 atctgacaag aagtttccaa acataaaaat gccgtccgaa cagctcagac ggcatttgtc
    12121 cattcggctt aaaccttatc catatccaaa cgcataaccg taacccattc accgttatgg
    12181 aaatgtcgcc cgacaaccgc ccagccgaat gattcataaa atatttgcac atcaggcgta
    12241 taaagataca agaactttat ccccagcgaa cgcgctgcgc ctatgcagtg ggcgaccagc
    12301 ctcctgccaa tgccttttcc gcgatattca ggtaaaacaa agacatcacc caaccaatat
    12361 tcatactgtg gaaaactttc catatcatgc cgcttgaccg tagccgaacc caacagggtt
    12421 ccggaatcat ccacagccgc aaaagccagc ggcagttcgt catccttcaa acacctgccg
    12481 taataggcat gaatcttatc cacagaagac cacggttcaa atccgtgcca ctcctcaaac
    12541 aacgcctgaa ccaacctgcc gatatgcccg gctttcagcc gtgtaatgaa aacagtattg
    12601 tctacaaaga gggaattcat cggtcaattc cccgacgctt tcgttccccc tgcgccgtaa
    12661 accgtattcc aagcatggtc caaacgcact ccgatttgcc tcagctcttc agcctgccgg
    12721 gctttttgcg ccattgctgc aggaatttcc gcttccaaac gggcgatgtc tgcttgagcc
    12781 gtctgcaaac gccggcgcgc atcttccaaa tccgactgca tcccgatgat ttttccgtcc
    12841 agattgtttt gtttttgcag taaggcacga tagccggatt ggatgctgag cagattgtct
    12901 tccgcatccc cggcccatac gcttgtagaa aaaacaaaca tcagaaaata caatattttt
    12961 ttcatgttca acttccgttt gaatgctgtc tgaagccgca ttccgacatc agacggcatc
    13021 gcccacgcct gtggataact taagcgcgga tgcgtttcaa cacttcttct ttgccgatta
    13081 atgccaacac agcatcgacg ctgggggttt tcgccgtacc gcagacggca aggcgcaggg
    13141 gcatgccgag tttgcccatt ttaatgcctt cttcgtcgca gaagggtttg aagaggtcgt
    13201 ggatggcttc ggcattccag tcttccagcc cttcgaggcg ttcggcaaag cgcagcatac
    13261 gggcggcggc ttcatcgtcc cagtgtttct gcacgtccgc ttcagtaggc gtttgtttga
    13321 cgtagaagta gaggcactcg tcggcaagtg tgttcaggtc ttgggcgcgg tctttgacca
    13381 gtgccaacac gtcttccaat gcaggttttt cagtttcatg gatatcgcgc agagcgagac
    13441 gcggtttgac gagttcggcg agtttgccgt tgggcgtgat tttgatgtgt tcgccgttga
    13501 tccagtagag tttcttcaaa tccatgcggc ttggagatgg agaaacgtct ttcaaatcaa
    13561 accattcgat aaattgttcc atcgtgaaga actcatcgtc gccgtgcgcc cagcccaaac
    13621 gtgccagata gttgagcatg gcttcgggca ggatgcccat cgcgccgaaa tcggtaatgg
    13681 caacggtatc gccgctgcgt ttggagattt ttttgccttg ttcgttgaga atcatgggca
    13741 ggtgtccgta ttcgggcagg tttgcgccga tggcttttaa gatgttgatt tgtttcgggg
    13801 tgttgttcac atggtcgtcg ccccggataa cgtgggtaac gcccatgtcg tagtcgtcca
    13861 ccacgacgca gaagttgtag gtcggtgtgc cgtcggcgcg tgcgataatc aggtcgtcga
    13921 gggcttcgtt ggggatggaa atttcgcctt tgaccaagtc cgcccacttg gttacgccat
    13981 ccaaaggcgt tttgaagcgg acgacgggtt gcacgccggc agggatttcg ggcagggttt
    14041 tgcccgcttc cggccgccag cggcggtcgt aagtcgccgt gccttctttt tcggctttct
    14101 cgcgcatggc ttccaactct tctttgctgc aatagcagta gtaggcatcg cctttttcta
    14161 aaagttcggc gatgacttct ttatagcggt cgaaacggcg ggtttggtac acgacgttgc
    14221 cggcgttgtc gtaatcgagt ccgacccatt tcatgccgtc gaggatgatg ttgacggatt
    14281 cggcggtaga acgcgccaag tcggtgtctt cgatgcgcaa taaaaattcg cctttatgat
    14341 ggcgggcaaa cgcccaagaa aacagggcgg tgcgaacgcc gccaatgtgc aggtagccgg
    14401 ttgggctggg ggcgaaacgg gttttgacgg tcatgatggc tccaaaatct ttaaaacggc
    14461 ttattttact ggttttaccg tgcttgggca tcaaaaatgc cgtccgaacc ctgcctgcgg
    14521 ataagtttca gacggcattt tctaccgctt caatcagaca gccatcccaa aaagcggctg
    14581 ccccattttt ccaaacggta gagggataac gcatatccct cttgcagcat aaagattttt
    14641 ttcttatttc ccgcatcaaa ccgcgtggtc ggggtggcag acatataaac gcggacaccc
    14701 aaatcctccg ccatttccgc cgcccgcgcc aaatggtagg gatcgctgac aatcaccacg
    14761 ctgtcaatac cgttggcacg caaaaccgga cggatgttgt tcaggttttc ataagtgttg
    14821 cgcgaagtgt tttcaaacag gatgttgcgc gccggaaccc cctgtttgag cgcgtaccgc
    14881 cgcccgacct cggcttcggt catatagcct tttttggttc gccctcccgt aaacacgatt
    14941 ttgcctaccc tgcggctctg ataaagcgcg atggcgtggt tgatgcgttc gcggaaaacc
    15001 ggggaagggc gtttgtccca cgcggcggcg cccaacacca gcgcggcatc cgcccggaca
    15061 tacggcggca aaacctgccc gcccgtccga taaaccgccc aaacggatga ggcaaacacc
    15121 agcaaaagcg gaaaaacact caaacagaaa ccgcccaaca ggtaatagcg caagccgttg
    15181 cggctgcaaa acagccgttt gttcacaata tcgcttcgat attttccaga ggcctgccga
    15241 cagccgcctt accgttcgcc aaaacaatcg gacgctccaa cagggcggga tgctcggcga
    15301 tggcacgcag cagcgcgtca ttgtccaaat cggggttgtc caaacccaat tccttgtaca
    15361 aatcatcttt cacacgcatc atcccgcgcg ccgatgccaa gcccaatttg ttgaaaatat
    15421 ccttcaattc ggacaagtcg ggcggcgtat ccaaatattt gaccacttcg gcagcaatgc
    15481 cgcgttcttc caatagggac aaggcggcac gcgatttgct gcaacgcgga ttgtggaaaa
    15541 ttttgatttc aggcatgaca tttccttgct tctcaacaat ccccttatta tcggcttacg
    15601 cagggtttta ctcaatatcc cgcccccaac cgtaccaaac ggtttacaat acccgaatcg
    15661 acatacaaag gacaaaacga tgaaatactt gaatcttgcc gcaatcgccc ttgccgccac
    15721 atttgccgca cataccgcct cggcagacga acttgccgga tggaaagacg gtaccgcgca
    15781 aagcctgcaa tcgctcaaag cccccgtacg catcgtcaac ctgtgggcga cttggtgcgg
    15841 cccgtgccga aaagagatgc ccgccatgtc caaatggtac aaagcgcaga aaaaaggcag
    15901 cgtcgatatg gtcggcatcg cgctcgacac atccgacaat atcggcaact tcctcaaaca
    15961 aactcctgtt tcctacccga tttggcgtta caccggggcg aacagccgaa actttatgaa
    16021 aacctacgga aacactgtcg gcgtactgcc ctttaccgtc gtcgaagcac cgaaatgcgg
    16081 atacaggcag accattaccg gggaagtgaa cgaaaaaagc ctgaccgaag ccgtcaaact
    16141 cgcccattca aaatgccgtt aaacgccgga tgccgtctga agccgcttca gacggcattt
    16201 ttcccgcccg accttcatat ccgccaaact tatccactat ctaaaagcag gcggaatctt
    16261 tataatcggc actgtcttac ctattgttca gacggcatat ccctgcggac gcaaccgccc
    16321 gaaacgatat gccgcccttc cttacaggac ctcctatgat ccgtttcgaa caagtttcca
    16381 aaacctatcc cggcggtttt gaagccctga aaaacgtcag cttccaaatc aacaaaggcg
    16441 agatgatttt tatcgcagga cactccggtt caggcaaatc caccgtcctc aagctgattt
    16501 cgggcattac caaaccaagc aggggcaaag tccacttcaa caaccaagac ctcggcacac
    16561 tgaccgacaa ccaaatcggc tttatgcgcc aacacatcgg catcgtgttc caagaccaca
    16621 aaatcctcta cgaccgcaac gtcctgcaaa acgtcatcct gccgcttcgg attatcggct
    16681 atccgccgcg caaagccgaa gaacgcgccc gcatcgccat cgaaaaagtc ggcctgaaag
    16741 gacgagaatt ggacgatccc gtaaccctct ccggcggtga acaacaacgc ctgtgcatcg
    16801 cccgcgccgt cgttcaccag cccggcctgc tgattgccga cgaaccctcc gccaacctcg
    16861 accgcgccta cgcgctcgac atcatggaat tgttcaaaac cttccacgaa gcgggaacaa
    16921 ccgtcatcgt tgccgcccat gacgaaaccc tgatggcgga ctacggacac cgcatcctgc
    16981 gcctctcgaa aggacgactc gcatgagcat catccactac gcctcgctgc acatcgaatc
    17041 cgcccgcacc gcgctcaaag agcttctgcg ccaacccttc ggcacgctgc tcaccctcct
    17101 gatgctcgcc gtcgcgatga ccctgccgct gtttatgtat ttgggcattc aaagcgggca
    17161 aagcgtgttg ggcaaactca acgagtcgcc gcaaatcacc gtctatatgg aaaccgccgc
    17221 cgcacaaagc gacagcgata ccgtccgcag cctgctggcg cgcgacaaac ggatcgccaa
    17281 cgtccgcttc atcggcaagg aagacggctt ggcggaatta cagtccaacc tcgaccaaaa
    17341 tttggtttcg atgctcgacg gcaacccgct gcccgacgca tttatcgtta cccccgaccc
    17401 cgcgacaacg cccgcgcaaa tgcaggcaat ctaccgggac attaccaaac tgcctatggt
    17461 cgaatccgcg tctatggata ccgaatgggt gcaaacgctg taccaaatca acgaatttat
    17521 ccgtaaaatt ttatggtttc tttccctgac gctgggtatg gcgttcgtcc ttgtcgcaca
    17581 caacaccatc cgcctgcaaa tcctcagccg caaagaagaa atcgaaatca ccaaactctt
    17641 gggcgcgccc gcatcgttca tccgccgccc cttcctttat caggcaatgt ggcagagcat
    17701 cctttccgcc gccgtcagcc tggggctttg cggttggctg ctttccacag tgcgcccctt
    17761 ggtcgatgcc atcttcaaac cctacgggct caatatcggc tggcgtttct tttatcctga
    17821 agaaatcgcg ctggtgttcg gcttcgtcat cgcgttgggc gtattcggcg cgtggcttgc
    17881 caccacccag cacctgctcg gcttcaaagc caaaaaataa aacaccgtca aaaatgccgt
    17941 ccgaacccgt tttcagacgg catttcaatt tgccagtata atggcgcatt tttccaacaa
    18001 ggaacctacc atgctgacct cggaacaagt aaaagccatg attgaaggcg tggcaaaatg
    18061 cgaacatatc gaagtagaag gcgacggaca ccattttttc gccgtcatcg tttcatcaga
    18121 atttgaaggc aaggcacgcc tcgcgcgcca ccgcctgatt aaagacggac tcaaagccca
    18181 actggaaagt aacgaactgc acgcactttc catttcggtt gccgccactc cggcggaatg
    18241 ggcagccaaa gcacaataat cgccacacaa aaatgccgtc agaaacagca caacgtttca
    18301 gacggcattt tttatatcaa accgcttacg cgccgcgttt ttccaaagcg gctacggcag
    18361 gcaactcttt accttccaag aactcaagaa acgcgcctcc gccggtagaa atatagccga
    18421 tttggtcggt aacgccgaat ttagcaatcg ccgccagcgt gtcgccaccg cctgcaatcg
    18481 agaatgcttt gctttgggca atggcttcgg caagagcttt cgtgccgcct gcgaactggt
    18541 caaactcgaa cacgccgacc ggtccgttcc aaacgaccgt gtcggcggct ttaagcaaat
    18601 cggcaagcgc ggcggcagat ttcgggccga tgtccaaaat catctcgtct tcggcaacgt
    18661 cggcaatgtc ttttaccacc gcttcggcat cggcggcaaa ggctttggca acgacgacat
    18721 cggtcggcag cggcacagaa cctcctttgg ccgccatttt cgccataatt tttttggatt
    18781 cttccaccaa atcgtgttcc gccaaagatt tgccgatggc tttaccttcc gccaacagga
    18841 aggtgttggc gataccgccg ccgacgatga gttggtcgac tttgtccgcc agcgattcga
    18901 ggatggtcag cttggtggac actttgctgc cggcaacgat ggcaaccatc gggcgcgcgg
    18961 gctgtttcaa ggctttgccc aaagcgtcga gttcgcccgc catcaatacg ccggcgcagg
    19021 caacgggagc ggcttgggcg acggcttcgg tcgaggcttg ggcgcggtgc gcggtgccga
    19081 acgcgtcatt gacgaacacg tcgcacaaag aagcgtaggc tttgcccagt tccaaatcgt
    19141 ttttcttctc gcctttgttg atacgcacgt tttgcagcat cacgacatcg cccgcgttca
    19201 aagccggttt gttttcgcgc cagtcgttca atactttcac gtttttgccc aacaggccgc
    19261 ccaaatgcgc ggcaacgggc gcaacgtcgt cttcggggtg aaactcgcct tcggtcgggc
    19321 ggcccaagtg ggtcatcacg atgacggacg cgccgttgtc cacgcagtat ttaacggacg
    19381 caatcgaggc gcggatacgg gtgtcgtcgc tgattttgcc gtctttgaac ggcacgttca
    19441 tatcggcgcg gatgaggacg gttttgccct gcacgttttg ttcggtcagt tttaaaaatg
    19501 ccataatcag tccttttcaa tcagtgtttg cgatacagaa acaattaatg cccttggatt
    19561 cggatttcaa gtgc
//
LOCUS       DAWFAN010000022        19504 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWFAN010000022 DAWFAN010000000
VERSION     DAWFAN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19504
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.022"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            1..191
                     /locus_tag="WGO65_000529"
                     /pseudo
     CDS             1..191
                     /locus_tag="WGO65_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            245..796
                     /locus_tag="WGO65_000530"
     CDS             245..796
                     /locus_tag="WGO65_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326865.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(1060..1609)
                     /locus_tag="WGO65_000531"
                     /pseudo
     CDS             complement(1060..1609)
                     /locus_tag="WGO65_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(2058..2615)
                     /locus_tag="WGO65_000532"
     CDS             complement(2058..2615)
                     /locus_tag="WGO65_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326866.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(2859..3017)
                     /locus_tag="WGO65_000533"
     CDS             complement(2859..3017)
                     /locus_tag="WGO65_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326867.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(3014..3856)
                     /locus_tag="WGO65_000534"
     CDS             complement(3014..3856)
                     /locus_tag="WGO65_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0326868.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNGPADERVLLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKIQRPQGYRLLGIFEEGKHNAVAVCGFHEACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            4094..5014
                     /gene="pyrB"
                     /locus_tag="WGO65_000535"
     CDS             4094..5014
                     /gene="pyrB"
                     /locus_tag="WGO65_000535"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ0326869.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            5024..5482
                     /locus_tag="WGO65_000536"
     CDS             5024..5482
                     /locus_tag="WGO65_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0326870.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            5547..6212
                     /locus_tag="WGO65_000537"
     CDS             5547..6212
                     /locus_tag="WGO65_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326871.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(6275..7492)
                     /locus_tag="WGO65_000538"
     CDS             complement(6275..7492)
                     /locus_tag="WGO65_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0326872.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            7634..8179
                     /gene="def"
                     /locus_tag="WGO65_000539"
     CDS             7634..8179
                     /gene="def"
                     /locus_tag="WGO65_000539"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ0326873.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            8266..9192
                     /locus_tag="WGO65_000540"
     CDS             8266..9192
                     /locus_tag="WGO65_000540"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ0326874.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDVPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDAGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            9271..10530
                     /gene="rsmB"
                     /locus_tag="WGO65_000541"
     CDS             9271..10530
                     /gene="rsmB"
                     /locus_tag="WGO65_000541"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ0326875.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAILRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIPARALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            10496..11095
                     /locus_tag="WGO65_000542"
     CDS             10496..11095
                     /locus_tag="WGO65_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ0326876.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            11095..13212
                     /locus_tag="WGO65_000543"
     CDS             11095..13212
                     /locus_tag="WGO65_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ0326877.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLISTASLSGNMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVETYA"
     gene            13205..14482
                     /locus_tag="WGO65_000544"
     CDS             13205..14482
                     /locus_tag="WGO65_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ0326878.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            14573..15760
                     /gene="dprA"
                     /locus_tag="WGO65_000545"
     CDS             14573..15760
                     /gene="dprA"
                     /locus_tag="WGO65_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ0326879.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            15786..16247
                     /locus_tag="WGO65_000546"
     CDS             15786..16247
                     /locus_tag="WGO65_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ0326880.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            16319..18625
                     /gene="topA"
                     /locus_tag="WGO65_000547"
     CDS             16319..18625
                     /gene="topA"
                     /locus_tag="WGO65_000547"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ0326881.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(18698..19393)
                     /locus_tag="WGO65_000548"
     CDS             complement(18698..19393)
                     /locus_tag="WGO65_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326882.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFQ"
BASE COUNT         4695 a         5005 c         5466 g         4338 t
ORIGIN      
        1 gaacgacaaa accatattaa tggaattgag aacttttgga accgggcaaa acgtcattta
       61 cgcaagttta acggcattcc caaagcgcat tttgagctgt atttaaagga gtgcgaatgg
      121 cgttttaaca acagtgagat aaaagttctt gttccatttt aaaacaatta gtaaaatcaa
      181 gtttgtccta gttatctagg acagcccctt gttttttgtt cggcggcttg cgtggtcggg
      241 taaaatgaaa gttttgaacg gttggtcgga caggaagatg tggcgggttt tgagtgcttt
      301 gccgataggc gtggtgtttt ttgatttgat ctacggtttt gtgttgaatg tgttgcaggg
      361 tttggatttg cagcgtgccg tgccggattc ggaaggcgtg ttggcggtta cgcccgatat
      421 tgcattcaac agtttgcaga ttgtcgccaa cggcggtatg gcggcggtgg tctgtttcgg
      481 gttggcggtt gtgtttttgc tcaaccgttc ggtgcggcgg cggcaggtgt tggaaatcgg
      541 ggtgttccgg atgttggggc tggtggcggt attggcgttc agcgcgccgt cggtgtggga
      601 gtgggcgaac gcgctgccgc tgctgctgaa gggcgcggac gtggtcaata cggggaatgc
      661 gcgttatgtg ctgacggctt tgtgtatgcc ctttccggcg gtgtcgtgcg tcatcgggct
      721 ggtggggcgg ttcaggcttc agacggcatc gggcagggcg gcaaagtcag ggggtgcggg
      781 caaggcggac ggataggacg catttttcag cgggtgcgtc gagaagcagc cgatgtgttt
      841 ggcagccgca gcttgggggg gtagtgctaa tggcggtttc tttgctttta tagtggatta
      901 acaaaaacca gtacggcgtt gcctcgcctt agcccaaaga gaacgattct ctaaggtgct
      961 caagcaccaa gtgaatcggt ttcgtactgt ttgtactgtc tgcagcttcg tcgccttgtc
     1021 ctgattacta tataaaataa atgggcaaaa atcggtttat tatcgttttt gccgcatttg
     1081 gatttgttct accgtaaaac gtgtttgacg aacgggattc ttattaaaaa aacatctgat
     1141 ttctaacaaa atcagtattt tttggcacga tggctaaaat ttttccttcc atttcgccat
     1201 cacgtgtttt ccatgcgctc aagaattgtg atttgctcat tgagacgtgc cccagcgatg
     1261 gatcagccag caaaacagtt tctccgttaa taccgttcaa taccgaaaaa tggttgtttt
     1321 tacggtattt taaatacaca attacaggaa tttttagttg taccaactgt tcaaatggca
     1381 aagcataacc ttgtgcttca aaacccagtt cgggcattat gcgttgcata tcatcaaaag
     1441 aagcacgcat ttgggtttta tccattttgt ctaagatttc cgcttcagaa taatgtctgc
     1501 cataaaaatt attcagtaac gtggcaatcg aagccgcgcc gcaagaaaaa tccaaatctt
     1561 gttttactat gccggaatct cgccgtgctt tccaactccg tacatggatg ttttggtaag
     1621 aagcgggggg tcaacaaaca taggccaagc aaaaactata tttggggcga aaccaatcaa
     1681 agccgcataa tttatcaatt tataaagatt ttttatcata atatgtatac gcggaataaa
     1741 cgcatgaaac ataaaaaaat aaaatcatat gcaattttat tgcatgaata aatatgaata
     1801 aaatagataa tgatgggagt aaatacgcca tgtattttgg aagtttaaat ttattaataa
     1861 taaaataatt tatcgtagcg caaataaatc ccaaaattgg aacgattaga aaaaaaatta
     1921 atacacccat catcatgcaa actctatatt aataaatagc taactaattc tattgtggaa
     1981 attagttagc taactaaaag ttattaatga ttattttcga gaattgactg cattgttggc
     2041 agcattggca ccaaaaccta gtgcatgaat acccggtctc catgccaaat tcccagccaa
     2101 tccgcctccg gcagcagcag ccagccctgt tttgccgcta ctcctgttgc cgcaccgatt
     2161 cctgccgcag tagccgcgcc ttgcgcagtt cctaatttac catgattata caaattagca
     2221 ccatgatact cccatgcacc taatgcaccg ccaaaagcag cggctgcaat aatgggaaca
     2281 aattcacctt gtgtttcttt catttcagcc tgtgataatt gaattgcttt cacattttgg
     2341 ctgtcaaaaa cttggctgtc taaattttgc gccattacag gtgtaatcat catagccatt
     2401 acagttgcaa ttttcgttgc gctggtttgc acataaatag gattagcaaa ttcgctttga
     2461 ttgcgttcag tgttgatgta gctaatactg ctttctagtt tgaatttacc cttgtcagtc
     2521 aataattctt ccaaacttaa aggtaagtca gcataagcaa tttggctggc aacagttaaa
     2581 ataaaatcta ttagacattt gtgtttttgc atcatttcgt ttgattttct agtttttgag
     2641 aatgatacaa agttttttac aaagtaaaga gtcactctga aaaacatttt ttcattataa
     2701 atcaaaatat tgatagaata aatagcgagc atcgattcac ggtgcacttt agtgcaaagc
     2761 gtacaacgcg agcctgaacc accagccgca acaggaaaag aaagccgata gagtgcaatg
     2821 cttgccaacg tgcaagcgag cttgcaagaa cgcttggctc aacgagagca ggcaagacag
     2881 aaagcagaaa gcaggatagg agcggtaacg caaaggtctc gggctttgat ttcgccgtaa
     2941 accctgctgc cgccttgtcc ggaaagggtg caggcggcga gtgccgacag ggtgcagatg
     3001 gggagggggg ttttcatttg gggtcgcaac ggaagtggta tgcgcagatt tcaaaaccgt
     3061 ttttgaaata caggcggtgc gcgtcggcac ggtcgtggtt gacgtggacg ttgaggtgga
     3121 ttttggttac ccctgtttcc gcgccgattt tgcggacttc ttccaaaagg cgcgaggcgt
     3181 agcctttgcg gcggctttgc ggcagggtaa cgatgtcgtc gatgtggatg tggcggccgc
     3241 cggcgagggt gcaggcttcg tggaagccgc agacggcgac ggcgttgtgt ttgccttctt
     3301 caaaaatacc cagcaggcgg tagccttggg ggcgttggat tttgttgatt tgttcggtaa
     3361 agcggttgat gtcggtcagg gcggaacgca aaacgctcaa ggctgcaaag gcggtggcgg
     3421 tgtcgtccgc gccgatttcg cgcaaaacgt aggatgcgcc cgaggcggtc tgttcctgtg
     3481 ctttttcggc ggcgtgtttt tcttcgattg cctgtgccag caggacgcgt tcgtcggcgg
     3541 ggccgttttg tccgccctgt tcgcgttctt cgagcagggc tttgcagtcg atgacgcgca
     3601 ggtcgttgtc ggcggcaaag tccatcagga agcggaacat ttggggattg tcggtttcca
     3661 gttttttatc gacggcgacg cagcggatgt tgtccaccag catggggcgc acccatttcg
     3721 agtaggagag gcggttgtct ttggtgaacg aggacaggat gccgcacagg cgttccgccc
     3781 agtcgctggg acggaaaatc ttgccggaac tcgttgtgcc gtggatgacg acttcgtagg
     3841 ggttgcagac taacatggcg gcttcctgaa aagaaatgtc tggcgcgatt ataccttatg
     3901 cttatgcggg cgtgtttgga tatgccgtct gaaaagtacg ggattcgtgc ggtaaaactt
     3961 tgcggcggca aatgtgcgat aatacgcgcc gtattgccgc ttttgcgaag ctgttccgca
     4021 aacatacggg cggcgtggac gacgtataac cggatacccg cctgacgcgg gttttttacg
     4081 gaagggggca aaaatgccta atccgcttta cagacagcac atcatctcga tttcggattt
     4141 gtcgcgcgaa cagttggaat gcctgcttca gacggcattg aagctgaagg cgcatccgcg
     4201 cggcgatttg ttggaaggca aacttatcgg ttcgtgcttc tttgaaccgt ccacgcgcac
     4261 gaggctgtcg tttgaaacgg cggtgcagcg gctgggcggc aaggtcatcg gcttttcgga
     4321 cggcgcgaat accagtgcca aaaaaggcga gacgcttgcc gataccgccc gcatcatttc
     4381 cggctacacc gacgcaatca tccaacgcca ccccaaagac ggcgcggcgc gcgtggcagc
     4441 ggagttttcg cgcgtccccg tcatcaacgc cggcgacggc accaaccagc accccagcca
     4501 gaccttgttg gatctggtaa cgatttatga aacgcagggg cgtttggaca agctcaaaat
     4561 cgccatggcg ggcgacttga aatacgggcg caccgtgcat tcgctttgtc aggcgttgaa
     4621 acgctggggc tgcgaatttg cctttgtttc gccgcccagc ctcgcgatgc ccgactacat
     4681 taccgaagag ttggacgaag ccggctgccg ataccgtatc ctcggcagtt tggaagaggc
     4741 ggcggaatgg gcggacatcc tgtatatgac ccgcgtccag cgcgaacgtt tcgatgaaca
     4801 ggaatttgcc aaaatccaag gcaaattcaa tctcgacgca tccatgcttg cccgcgccaa
     4861 accgaacctg cgcgtgctgc accctttgcc gcgcgtggac gaaatccatc ccgatgtcga
     4921 tgctacgccg catgcctatt atttcgagca ggcgaccaac ggcgtttatg cgcgtatggc
     4981 gatattgtcg ctggtgttga acgaagaagt gtgaggaacc gatatggaaa ccccgaaact
     5041 cagtgtcgaa gccattgaaa aaggtacggt tatcgaccat attcccgccg gcagggggct
     5101 gaccatcctg cgccagttca aacttttgca ctacggcaac gcggtaaccg tgggcttcaa
     5161 cctgcccagc aaaacccaag gcagcaaaga catcatcaaa atcaaaggcg tgtgcttgga
     5221 cgacaaagcc gccgaccgcc tcgccctgtt cgcccccgaa gcggtggtca ataccatcga
     5281 caatttcaag gtcgtgcaga agcggcattt gaacctgccc gacgaaatcg ccgaagtgtt
     5341 ccgctgtccg aacacgaatt gcgccggcca cggcgagccg gtcaaaagcc ggttttatgt
     5401 taaaaaacac aacgggcaga cgcggctgaa atgccattac tgcgaaaaaa cctacagccg
     5461 ggattcggtg gcggaagcct gacggattcc cttaaaccga gcgggcggca tttcgtctgc
     5521 cgcttgtttt gccaatctga aatggaatga tgatgcacgc ttctgtccaa agccgtttcg
     5581 caccgatact ttatgttttg attttctttg ccggtttttt gaccgcgcaa atctggttca
     5641 atcagaaagc ctatactgaa gagctgcctc cgcttctgtc cgcattgtcc gctgtcgcgc
     5701 tggtgtggct ggcgtgggcg ttcgtgtcgg cgcgttcaaa ggctaaggcg gaaaagttct
     5761 accgcgaaaa aatgatacag aacgaaagca tacatcccgt cctgcacgct tctttgcaac
     5821 acttggaaca caagccgcaa atgctcgccc tgctggtcaa aaaccacggc aaagggatgg
     5881 cggaacaggt caggttcaag gcggaagtgc tgcccgacga cgaagacgcg cgcacgattg
     5941 ccgccgagtt ggcaaaaatg gatatgtttg cattggggac ggacgcggtc gcctcgggcg
     6001 aaacctatgg acgcgtgttc gccgatattt tcgagttgtc ggcggctttg gaagggcgcg
     6061 cgttcaaagg aatgttgaaa ctgacggcgg aatataaaaa catcttcggc gatgcctgcc
     6121 gttcggaaac ggcgttggag ttgggcgcgc tcaatcaggc gttgcaggag atttcaaaaa
     6181 catcggaaaa gtccaaacgg atattttatt gaagatggaa aaatgccgtc tgaaacggaa
     6241 ggtgtttcag acggcatttt tgtcggatga ttaattattc ggagcggttg aagccaaact
     6301 tcacgcggct gcggccctga tccggtatat tgtccaaatc gcgtcccgga ttggcggcgg
     6361 tgtcgcctac ggaaatatcg gagatgtttt ccaaaatgat ggcggacgac aggtgttcgg
     6421 aggtgcggta aaccattgcc aagcccactt cttcggcagg agtggaaatc agctcgacgg
     6481 tatccctgct tttgaaattg ttggagaggt cgacctgcat cgttttcttg cgtttgtaga
     6541 ggctcaaaac cgtgcctttg tccaaaccgt ccgcctcgcc tttgtcgatg gtgatggttt
     6601 gaaactggcc ggcaatcctt gtgccttcaa acacggaaac gattttagcc tgaaccgggc
     6661 gggacggttc gtgcggcatc atgttgaagc ggtcggtgtc ttccggcatt ttcatcaggt
     6721 agtcgccctg ctgtatttcg gaaatggcgg tttcgaccac cagcggctgt atcgaagggg
     6781 tgcgcagcgg ggtaatcaaa ggatgggtgc gggtatggta ttcgttgtct ttcggccgtt
     6841 ctccagcctg tttcgagcgt tgttcgagga cagagtcggt atagtcgagg gagcgcacga
     6901 tgccgctgaa tgcgacttcc tgcccgagga atttacccgt atccggatcg gtgatgtttt
     6961 tattgattcg gtaggtcagg tagcggcccg gctctttcag gcctttggtg taaaccctgg
     7021 tgcctttggt gtacagcagc ctgccttccg ggcccgagag caggcgcggc gcggcagcgg
     7081 tttctttgcg ggaaacgatt tgcgggtgcc gcataaagat gcggtagaag ttgacatcga
     7141 tggcgggaat accgtatccg gacacttcct tatccggact cattttgacg acggggatgc
     7201 cgtctgtctg ttccaagccg aggcgcggtt cgccgtcaac gtggcgcaac accaatacct
     7261 ggtccggata aatcaggtcg ggattgtgga tttgatcccg gttcgcgtcc cacaggcggc
     7321 cccattgcca cgggctgtac aggtatttgc ccgaaatgcc ccacagggtg tcgccctgtt
     7381 tgaccgtgta gcgttccggc gcgttcgggc gcacctccaa atttgccgcc aaagtttgtg
     7441 ttgagaatgc catacctgcc gcgcagagca gggttataat acgacgttgc ataaccgttc
     7501 cccttatctg ataaatttcg gtttgtcttg cttgattggg ttggaaaaag cggcggcagc
     7561 ccctcgggat gtgccgcgtg ataaaaaatg ttccgcattt taacatcgaa ttatccgcac
     7621 catcacggta attatgaaaa acaggcggcg tatccgccga aggaaagaga aaattatggc
     7681 tttattgaat atcttgcaat atcccgacga gcgtctgcac acggtggcaa agcctgtcga
     7741 acaagtcgac gagcgcatcc ggaagctgat tgccgatatg tttgaaacga tgtacgaatc
     7801 gcgcggcatc gggctggcgg cgacgcaggt cgatgtgcac gagcgcgtgg tcgtgatgga
     7861 tttgaccgaa gaccgcagcg aaccgcgcgt gttcatcaac cccgtcatcg ttgaaaaaga
     7921 cggcgaaacc acttacgaag agggctgcct gtccgtgccg ggcatttacg acaccgtaac
     7981 ccgcgccgaa cgcgtcaagg tcgaggcttt gaacgaaaaa ggcgaaaagt tcacgctgga
     8041 ggcggacggc ttgctggcga tttgcgtgca gcacgagttg gaccacctga tgggtatcgt
     8101 gtttgtcgaa cgcctttccc aactcaagca ggggcggatt aagaccaagc tgaaaaaacg
     8161 tcagaaacat acgatttgac ccttttgccg tgccgtctga acgctgcaaa gttttcagac
     8221 ggcacggtct tgtccgacaa ttttacgcac acgcaggaac acgctatgaa agtcatcttc
     8281 gccggcacgc ccgattttgc cgccgccgcc ttaagagccg ttgccgccgc cggttttgaa
     8341 atcccgctgg tgctgaccca gcccgaccgt ccgaaagggc gcggtatgca actgactgcc
     8401 ccgcccgtca aacaagccgc gctggaactc ggtttgcgcg tcgaacagcc cgaaaaactg
     8461 cgtaacaacg ccgaagccct gcaaatgctc aaagaggtcg aggcagacgt aatggtggtt
     8521 gccgcctacg gtttgattct gccgcaagac gtgttggatg tgccgaaaca cggctgcctc
     8581 aacatccacg cttcgctgct gccccgttgg cgcggcgcgg cgccgattca gcgcgcgatt
     8641 gaggccggcg atgccgaaac cggcgtgtgc attatgcaga tggatgccgg tttggacacc
     8701 ggtgatgtgg tcagcgaaca ccgctacgcc atccaaccga ccgataccgc caacgaagtc
     8761 cacgacgcgc tgatggaaat cggtgcggcg gcggttgttg ccgatttgca acagcttcaa
     8821 agcaaaggcc gtctgaacgc ggtcaaacag cccgaagaag gtgttactta cgcgcaaaaa
     8881 ttgcgcaaag aagaggcgcg tatcgattgg agcgaaagcg cggcggttat cgaacgcaaa
     8941 atccgcgctt tcaaccccgt gcctgccgcg tgggttgagt atcagggcaa gccgatgaaa
     9001 atccggcggg cggaagtggt ggcgcaacaa ggcgcggcag gcgaagtgtt gtcctgttcg
     9061 gcggacggtt tggtcgttgc ctgcggcgaa aacgcgctga agattaccga attgcagcct
     9121 gccggcggca ggcgtatgaa tatcgcggcg tttgcagcag gacggcatat cgaagcaggg
     9181 acgaagctgt aaatcccttc agacggcatt ccgatccgca aacgggaatg ccgcctgaaa
     9241 ccatcagttg aagaaagcga atcacataat atgagtatgg cacttgccca aaaacttgcc
     9301 gccgacagca ttgcggcggt tgccgaagga cgcaaccttc aggacgtgtt ggcgcaaatc
     9361 cgcaccgcgc atcccgacct tacggcacag gaaaacggcg cgttgcagga catcgcctac
     9421 ggctgccagc gttatttggg cagtttgaaa catatgctcg cgcagatgct gaaaaagccg
     9481 attgacaacc cgcagctcga aagcctgctt ttggcggcgt tgtaccaact gcattacacg
     9541 cgcaacgcgc cgcacgccgt ggtcaacgaa gcggtggaaa gcatagccaa aatcggacgc
     9601 gggcagtacc gttcgtttgc caacgcgatt ttgcgccgct ttttgcgcga acgcgacaag
     9661 cttgcggctt cctgtaaaaa agacgatgtg gcgaaacaca acctgccgct gtggtgggtg
     9721 gcttacctga aaaaccatta tccgaaacac tggcacaaca tcaccgccgc gctacaatcc
     9781 catccgccga tgacgttgcg cgtcaaccgc cgacacggca atgccgaaag ctatttggaa
     9841 aaactggctg cggaaggtat tccggccagg gcgttggacg aatatgcggt tacgttggaa
     9901 gaagccgtgc cggtaaaccg tctgcccggt ttttcagacg gcattgtttc ggtacaggac
     9961 ttcggtgcgc agcaggcggc gtatcttctt aacccgaaag acggcgaacg gattttggac
    10021 gcgtgcgccg cgccgggcgg caagacgggg catatcttgg aactggcgga ttgccgcgtt
    10081 accgccttgg acatcgatgc aggccgtctg aagcgtgtgg aggacaatat cgcccgtttg
    10141 ggctttcaga cggcatcggc ggcgtgtgcc gatgcgcggg atttggcggc gtggtatgat
    10201 ggaaagcctt tcgacgcaat ccttgccgac gtgccatgca ccgcttccgg cgtggcgcgg
    10261 cgcaatcccg acgtgaaatg gctgcgccgt ccgaccgacg cgctcaaaac cgcccgccag
    10321 caggaagccc tgctggacgc actttggcag acgctgaaac agggcggcag aatgctgctt
    10381 gccacctgtt ccgtgttcgt cgaagaaaac gacgggcaat tgcaaaaatt cctcaaccgc
    10441 catgccgatg ccgaaccgat cgaatcgcgg gtgcttttac cgaacaaaca ccaagatggc
    10501 ttttattacg cgcttattca aaagcattaa acaatggctt gtgctgctgc cgatgctttc
    10561 cgttttgccg gacgcggcgg cggaggggat agatgtgagc cgcgccgaag cgaggataac
    10621 cgacggcggg cagctttcca tcagcagccg cttccaaacc gagctgcccg accagctcca
    10681 acaggcgttg cgccggggcg tgccgctcaa ctttacctta agctggcagc tttccgcccc
    10741 gataatcgct tcttatcggt ttaaattggg gcaactgatt ggcgatgacg acaatattga
    10801 ctacaaactg agtttccatc cgctgaccaa ccgctaccgc gttaccgtcg gcgcgttttc
    10861 gacagactac gacaccttgg atgcggcatt gcgcgcgacc ggcgcggttg ccaactggaa
    10921 agtcctgaac aaaggcgcgc tgtccggtgc ggaagcaggg gaaaccaagg cggaaatccg
    10981 cctgacgctg tccacttcaa aactgcccaa gccatttcaa atcaatgcat tgacttctca
    11041 aaactggcat ttggattcgg gttggaaacc tctaaacatc atcgggaaca aataatgcgc
    11101 cgttttctac cgatcgcagc catatgcgcc gtcgtcctgt tgtacggact gacggcggca
    11161 accggcagca ccagttcgct ggcggattat ttctggtgga tagttgcgtt cagcgcaatg
    11221 ctgctgctgg tgttgtccgc cgttttggca cgttatgtca tattgctgtt gaaagacagg
    11281 cgcgacggcg tattcggttc gcagattgcc aaacgccttt ctgggatgtt tacgctggtt
    11341 gccgtactgc ccggcgtgtt tctgttcggc gtttccgcac agttcatcaa cggtacgatt
    11401 aattcgtggt tcggcaacga tacccacgag gcgcttgaac gcagcctcaa tttgagcaag
    11461 tccgcattga atctggcggc agacaacgcc cttggcaacg ccatccccgt gcagatagac
    11521 ctcatcagca ccgcctccct atccggcaat atgggcaggg tgctggaaca ttatgccaac
    11581 gggtttacac agttgtccct ttacaatgcc gcaagcggca aaatcgaaaa aagcatcaac
    11641 ccgcacaagc tcgatcagcc gtttccggat aaggcgcgtt gggaaaaaat ccaacaggcg
    11701 ggttcggtca gggatttgga aagcataggc ggcgtattgt acgcgcaggg ctggctgtcg
    11761 gcgggtacgc acaacgggcg cgattacgcc ttgtttttcc gtcagccggt tcccaaaggc
    11821 gtggcagagg atgccgtctt aatcgaaaag gcaagggcga aatatgctga gttgagttac
    11881 agcaaaaaag gtttgcagac ctttttcctg gcaaccctgc tgattgcctc gctgctgtcg
    11941 atttttcttg cactggtcat ggcactgtat ttcgcccgcc gtttcgtcga acccgtccta
    12001 tcgcttgccg agggggcgaa ggcggtggcg caaggcgatt tcagccaaac ccgtcctgtt
    12061 ttacgcaatg acgagttcgg gcgtttgacc aagttgttca accacatgac cgagcagctt
    12121 tccatcgcca aagaagcaga cgagcgcaac cgccggcgcg aggaagccgc cagacattat
    12181 cttgaatgcg tgttggaggg gctgaccacg ggcgtggtgg tgtttgacga acaaggctgt
    12241 ctgaaaacct tcaacaaagc ggcggaacag attttgggga tgccgcttac ccccctgtgg
    12301 ggcagcagcc ggcacggttg gcacggcgtt tcggcgcagc agtccctgct tgccgaagtg
    12361 tttgccgcca tcggcgcagc ggcaggtacg gacaaaccgg tccatgtgaa atatgccgcg
    12421 ccggacgatg ccaaaatcct gctgggcaag gcaaccgtcc tgcccgaaga caacggcaac
    12481 ggcgtggtaa tggtgattga cgacatcacc gttttgatac acgcgcaaaa agaagccgcg
    12541 tggggcgaag tggcaaaacg gctggcacac gaaatccgca atccgctcac gcccatccag
    12601 ctttccgccg aacgtctggc gtggaaattg ggcgggaagc tggatgagca ggatgcacaa
    12661 atcctgacgc gttcgaccga caccatcgtc aaacaggtgg cggcattgaa ggaaatggtc
    12721 gaagcattcc gcaattatgc gcgttcccct tcgctcaaat tggaaaatca ggatttgaac
    12781 gccttaatcg gcgatgtgtt ggcattgtat gaagccggtc cgtgccggtt tgcggcggag
    12841 cttgccggcg aaccgctgat ggtggcggcg gatacgaccg ccatgcggca ggtgctgcac
    12901 aatattttca aaaatgccgc cgaagcggcg gaagaagccg atgtacccga agtcagggta
    12961 aaatcggaag cggggcagga cggtcggatt gtcctgacgg tttgcgacaa cggcaaaggg
    13021 ttcggcaggg aaatgctgca caacgccttc gagccgtatg taacggacaa accggcggga
    13081 acgggattgg gtctgcccgt ggtgaaaaaa atcattgaag aacacggcgg ccgcatcagc
    13141 ctgagcaatc aggatgcggg cggcgcgtgt gtcagaatca tcttgccaaa aacggtagaa
    13201 acttatgcgt agcagcgata ttttaattgt agacgacgaa atcggcatcc gcgacctgct
    13261 gtcggaaatc ctgcaggacg aaggttattc ggtcgcattg gcggaaaacg ccgaagaggc
    13321 gcgcaagctg cgccatcagg cgcgtccggc gatggtgctg ctggatattt ggatgcccga
    13381 ttgcgacggc atcacccttt tgaaggagtg ggcgaaaaac gggcagctca atatgccggt
    13441 ggtgatgatg agcgggcacg ccagcatcga taccgccgtg gaagccacca aaatcggcgc
    13501 gatcgatttt ttggaaaaac cgatttccct gcaaaagctg ctgtctgccg tcgaaaacgc
    13561 gttgaagtac ggtgcggcgc aaaccgaaac ggggcctgta ttcgacaagc tgggcaacag
    13621 tgcggcgatt caggaaatga accgtgaggt aggggctgcg gtgaaacgtg cctctcccgt
    13681 acttttgacg ggcgaggcag gttcgccgtt tgaaacggtg gcacgctatt tccataaaaa
    13741 cggtacgccg tgggtcagcc cggcaagggt cgaatatctg atcgatatgc cgatggaact
    13801 gttgcagaag gcggagggcg gcgttttgta tgtcggcgac atcgcccagt acagccgcaa
    13861 catccaagcc ggtattgcct ttattgtcgg caaggcggaa caccgccgcg tcagggtggt
    13921 cgcatcgggc agcagggcgg caggttcaga cggcattgcc tgcgaggaaa agctggcgga
    13981 actgctgtcg gagtcggtcg tccgcattcc gccgctgcgt atgcagcatg aagatattcc
    14041 cttcctgata caggggattg cctgcaatgt ggcggaaagt caaaagattg cgcccgccgc
    14101 attcagtgaa gaggcacttg ccgcattgac ccgttacgac tggccgggaa atttcgacca
    14161 actgcaaagc gtcgttgcaa cgctgttgtt ggaggcggac ggacaggaaa tcggcgcggc
    14221 ggcggtttct tcccttttgg ggcagaatgt gcctgccgag ggagcggaag atatggtggg
    14281 cgggtttaat ttcaacctgc ccctgcgcga attgagggag gaggtggagc ggcgttattt
    14341 cgagtaccac atcgcccaag aaggtcagaa tatgagcaaa gtggcgcaga aggtcggttt
    14401 ggaacgcaca cacctttacc gcaaactcaa acagctcggc atcggcgttt cgcgccgggc
    14461 gggggaaaaa accgaagaat aggcccggac ggtcggtttt ccggctgcgg gcttttgttt
    14521 tcagacggca tttggtgcaa atgccgtctg aaatcgtaag gggacggatt ttatgacaga
    14581 ggacgaacgt ttcgcgtggt tgcaactggc ttttacgccc tatatcggtg cggaaagttt
    14641 cttgctgctg atgcgccgtt tcggcagcgc gcaaaatgcc ttgtccgcac cggcggaaca
    14701 ggtcgccccg gcggtgcggc acaagcaggc ggccgaggct tggcgcaatg cggaaaaacg
    14761 cgctttggcg cggcaggcgg cagaagcggc gttggaatgg gaaatgcggg acggatgccg
    14821 cctgatgctg cttcaggatg aagatttccc cgaaatgctg acgcaggggc tgaccgcgcc
    14881 gccggttttg tttttgcgcg gcaacgtgcg gctgctgcac aagccttccg ccgccatcgt
    14941 cggcagccgc catgccacgc cgcaggcgat gcggattgcc aaagatttcg gcaagtcgtt
    15001 gggcgggcaa aatatccccg ttgtgtcggg tatggcttcc ggtatcgata ctgccgccca
    15061 tcagggcgca ctggaggcag aaggcggcac catcgccgtg tgggggacgg gtatagaccg
    15121 catttacccg ccgtccaata aaaaccttgc ctatgaaatc gccgaaaaag gattgattgt
    15181 cagcgagttt cccatcggta cgcgcccgta tgccggcaac tttccgcgcc gcaaccgcct
    15241 gattgccgcg ctgtcgcagg ttacgctggt ggttgaagct gcgttggaat ccggttcgct
    15301 gattaccgcc ggcttggcgg cagaaatggg gcgcgaagtg atggcagtac ccggttcgat
    15361 agacaatccg cacagcaaag gctgccacaa actgattaaa gacggcgcga aattggtgga
    15421 atgcctggac gacatcctga acgaatgccc ggggctattg caaaatacgg gtgcttcatc
    15481 atattctata aataaggata cgcccgacac gggacgccgc gccgttcaga cggcatacgc
    15541 cccgccgcct gccgcaaaaa tgccgtctga agcggcggca ggcggcacag ccgtcggcgg
    15601 catactggat aaaatgggtt tcgaccccat ccatcccgac gtgcttgccg gacagttggc
    15661 tatgcctgcc gcagatttgt atgccgcact gttggaattg gaattggacg gcagcgttgc
    15721 cgcaatgccc ggcggcagat accagcgtat ccgaacttga acgcacttta tattaaggaa
    15781 cacgaatgac cgaagtcatc gcctacctca tcgaacattt ccaagatttc gatacctgtc
    15841 cgccgcccga agacttgggt atgctgcttg aagaagcggg tttcgatacg atggaaatcg
    15901 gcaacaccct gatgatgatg gaagtattgc tcaacagctc cgaattttcc gccgaacctg
    15961 ccgacagcgg cgcattgcgc gtgtacagca aagaagaaac cgacaacctg ccgcaggaag
    16021 tgatggggct gatgcagtat ctgattgaag aaaaagccgt cagctgcgaa cagcgggaaa
    16081 tcatcatcca cgcgctgatg cacatccccg gcgacgaaat taccgtagat accgccaaag
    16141 tgctgaccct gctgctttta tgggcaaaca agagcgagct gcccgtgttg gtcggcgacg
    16201 agctgatgag cgcgctttta ctcgacaaca aacccacgat gaactgaagc ggcttcagac
    16261 ggcccgcccg aggccgtctg aaacgccggc atcaaaacca ccatccagag aacgacaaat
    16321 ggcgaaaaac ctattaatcg tcgaatcccc gtccaaagcc aaaaccctga aaaaatattt
    16381 gggcggcgat tttgaaatcc ttgcatccta cggacacgtc cgcgacctcg tccccaaaag
    16441 cggcgcggtc gatcccgaca acggctttgc gatgaaatac caactcatca gccgcaacgg
    16501 caaacacgtc gatgccattg tcgccggtgc caaagaagct gaaaacatct acctcgccac
    16561 cgacccggat agggaaggcg aagccatttc ctggcatctt ttggaaatcc tcaaatccaa
    16621 acgcggcttg aaaaacatca agccgcagcg tgtcgtgttc cacgaaatca ccaaaaacgc
    16681 cgtgctcgat gccgttgccc atccgcgcga aatcgaaatg gacttggtcg atgcgcaaca
    16741 agcccgccgt gctttggact atttggtcgg ctttaacctt tcgccattgt tatggaaaaa
    16801 aatccgccgc ggtttgagcg cgggtcgtgt gcaaagtccc gctttgcgcc tgatttgcga
    16861 acgcgaaaac gaaatccgcg cgtttgaagc gcaggaatat tggacggtac atctagacag
    16921 ccacaaaggc cgcagcaagt tcaccgccaa actcgcccaa tacaacggcg cgaaactcga
    16981 acaattcgac ctgccgaacg aagccgctca agccgatgtg ttgaaagaac tcgaaggcaa
    17041 agaggccgtc gttaccgcca tcgaaaagaa aaagcgcagc cgcaatcctg ccgcgccgtt
    17101 caccacttct accatgcagc aggatgccgt gcgcaaactc ggcttcacca ccgaccgcac
    17161 catgcgtacc gcccaacagc tttatgaagg tatagacgta gggcagggcg cgatcggtct
    17221 gattacctat atgcgtactg acagcgtgaa tttggcggat gaagcgttaa ccgaaatccg
    17281 ccattacatc gaaaacaaaa tcggcaaaga atatctgccg agtgccgcca aacaatacaa
    17341 aaccaaatcc aaaaacgccc aagaagcgca cgaagccatc cgtccgactt ccgtgtaccg
    17401 cacgcccgaa agcgtcaaac ccttcctgag cgcagaccag ttcaaactct atcaaatgat
    17461 ttggcagcgt accgtcgcct gtcagatgac gcctgccaaa ttcgaccaaa ccaccgtcga
    17521 tattaccgtc ggcaaaggcg tattccgcgt aaccggacaa gtgcaaacct tcgcaggctt
    17581 cttgagcgtt tatgaagaaa gcagcgacga tgaagaaggc gaagacagca aaaaactgcc
    17641 cgaaatgagc gaaggcgata aattgcccgt ggacaaactc tacggcgaac aacactttac
    17701 cactccgccg ccgcgctaca acgaagccac gctggttaaa gccctcgaag aatacggcat
    17761 cggccgcccc tcgacctacg ccagcatcat ctccacgctc aaagaccgcg aatacgttac
    17821 ccttgagcaa aaacgcttca tgcccaccga cacaggcgac atcgtcaaca aattcctgac
    17881 cgaacacttc gcccaatacg tcgattacca cttcaccgcc aaactcgaag accagcttga
    17941 cgaaattgcc gacggcaaac gccaatggat tcccttgatg gacaaattct ggaaaccgtt
    18001 catcaagcaa gtggaagaaa aagaaggcat cgaacgcgcc aaatttacca cgcaggaact
    18061 tgatgaaacc tgcccgaaat gcggcgaaca caaactgcaa atcaaattcg gcaaaatggg
    18121 tcgttttgtt gcgtgtgccg gttatcccga gtgcagctac acgcgcaacg tcaacgaaac
    18181 tgccgaagaa gccgccgagc gcatcgccaa agccgaagcc gaacaggccg aactcgacgg
    18241 acgcgagtgc ccgaaatgtg gcggtcgcct agtgtacaaa tacagccgca ccggcagcaa
    18301 attcatcggc tgcgccaact accccaaatg caaacacgtc gagccgctgg aaaaaccgaa
    18361 agataccggc gtccagtgtc cgcaatgcaa aaaaggcaac ctcgtcgagc gcaaatcccg
    18421 ctacggcaaa ctgttttaca gttgcagcac ctatcccgac tgcaactacg ccacttggaa
    18481 cccgcccgtt gccgaaagct gtccaaaatg cgcatggccg gttttgacca tcaaaaccac
    18541 caaacgctgg ggtgtggaaa aagtttgtcc gcaaaaagaa tgcggctgga aagaacagat
    18601 tgaaccgcct gcaccgaaag agtgagatta ggttggtttg aaagagaaaa ggtcgtctga
    18661 aaaattttca gacgaccttt gcttttctgt gattggttta ttggaatccg cgtgttgttt
    18721 taaagtccga taaaatccgg ttcatttcag gcgcaaacaa ggcgatgtaa tcgtaagata
    18781 gaccgcgact ggcactggga tggggaaagc agacgacttc gcaatcttca aacgattgga
    18841 atttgacatt gaaacgtgta ccgtcaaatt ctttttgcac cgtctccagc ggtttggtct
    18901 gcttaccgac caactgctcg aagcgtggca gtacattttg gttgttcaga aaatccgcca
    18961 acctgctgcc catgaagagg atgactttcg gacgcagttt ttcgatgtgg tagagaaaat
    19021 tatcgatgtg ctcgggttgt gtgaacttgt cgggattgtc gatagtgttg ccctgtgtag
    19081 cagcccagtt ggtttgaacc agggattttt caaatgcacc gcccaatcca ttttcgtcta
    19141 aggggtgtcc ccacatttca aaccaatttt ttatcgtatt atcgtaacgc cacttttttg
    19201 cctgctctcc gaaatagagg gatttgtttg caaatgtatg gtcgattttg ttttcaggga
    19261 gtttgtattc acctgctaca taagcagcct catcggcttt actccaaccc cattcatagc
    19321 cacaaatcat taagccatgt ttgtcgttgt agcctttgaa caggctgttg ctcaaattca
    19381 aatccttcat catgaactct tccttttaaa atttaagagc gattgacttc aatgttttta
    19441 gatggggtgg aaaaatcctt gtgtaggcaa cataaattca ataaatttct tgataattcg
    19501 aaac
//
LOCUS       DAWFAN010000023        19191 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWFAN010000023 DAWFAN010000000
VERSION     DAWFAN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.023"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(220..1995)
                     /gene="ptsP"
                     /locus_tag="WGO65_000549"
     CDS             complement(220..1995)
                     /gene="ptsP"
                     /locus_tag="WGO65_000549"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ0326883.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIAKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(1995..2264)
                     /locus_tag="WGO65_000550"
     CDS             complement(1995..2264)
                     /locus_tag="WGO65_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ0326884.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2333..2770)
                     /locus_tag="WGO65_000551"
     CDS             complement(2333..2770)
                     /locus_tag="WGO65_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ0326885.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2851..3414)
                     /locus_tag="WGO65_000552"
     CDS             complement(2851..3414)
                     /locus_tag="WGO65_000552"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ0326886.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3483..4307)
                     /locus_tag="WGO65_000553"
     CDS             complement(3483..4307)
                     /locus_tag="WGO65_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ0326887.1"
                     /translation="MIKKTIGGIIPIFMAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISAAVRS
                     ASSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRARTDR"
     gene            4399..5031
                     /locus_tag="WGO65_000554"
     CDS             4399..5031
                     /locus_tag="WGO65_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ0326888.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5237..7021)
                     /gene="rmuC"
                     /locus_tag="WGO65_000555"
     CDS             complement(5237..7021)
                     /gene="rmuC"
                     /locus_tag="WGO65_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ0326889.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     QGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(7395..8180)
                     /locus_tag="WGO65_000556"
     CDS             complement(7395..8180)
                     /locus_tag="WGO65_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ0326890.1"
                     /translation="MKQTLKNWFAALLLAVPMSAAVASGGGHYEKVDIDLRDQVSLQH
                     GAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDAA
                     KWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQG
                     VQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVNY
                     LVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLNKEFSKDIH"
     gene            complement(8195..9544)
                     /locus_tag="WGO65_000557"
     CDS             complement(8195..9544)
                     /locus_tag="WGO65_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ0326891.1"
                     /translation="MANQTNSKAKALLGWVDARFPLSKMWKEHLSEYYAPKNFNFWYF
                     FGSLSMLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKLKDENGVPLDGIPFFPYYVVHDILAVTIFLIVFCAVMFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFAGTQVWGVIGMGA
                     AVVLIALLPWLDKGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITVVGAYLFATNI"
     gene            complement(9563..10144)
                     /gene="petA"
                     /locus_tag="WGO65_000558"
     CDS             complement(9563..10144)
                     /gene="petA"
                     /locus_tag="WGO65_000558"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ0326892.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10267..11016)
                     /locus_tag="WGO65_000559"
     CDS             complement(10267..11016)
                     /locus_tag="WGO65_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ0326893.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11147..12076
                     /locus_tag="WGO65_000560"
     CDS             11147..12076
                     /locus_tag="WGO65_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0326894.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAEWDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNDIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(12332..12724)
                     /gene="rpsI"
                     /locus_tag="WGO65_000561"
     CDS             complement(12332..12724)
                     /gene="rpsI"
                     /locus_tag="WGO65_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ0326895.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12734..13165)
                     /gene="rplM"
                     /locus_tag="WGO65_000562"
     CDS             complement(12734..13165)
                     /gene="rplM"
                     /locus_tag="WGO65_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ0326896.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13343..13522)
                     /gene="ssrS"
                     /locus_tag="WGO65_000563"
     ncRNA           complement(13343..13522)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WGO65_000563"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13528..13833)
                     /locus_tag="WGO65_000564"
     CDS             complement(13528..13833)
                     /locus_tag="WGO65_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ0326897.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13844..14173)
                     /locus_tag="WGO65_000565"
     CDS             complement(13844..14173)
                     /locus_tag="WGO65_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326898.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14228..15217)
                     /locus_tag="WGO65_000566"
     CDS             complement(14228..15217)
                     /locus_tag="WGO65_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ0326899.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15340..18042)
                     /gene="ppc"
                     /locus_tag="WGO65_000567"
     CDS             complement(15340..18042)
                     /gene="ppc"
                     /locus_tag="WGO65_000567"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ0326900.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEELARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVTELFQHAGLEDYNSLNEEQKQAVLLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18085..18978
                     /locus_tag="WGO65_000568"
     CDS             18085..18978
                     /locus_tag="WGO65_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ0326901.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADFDTVELHNLQRQVAFDEGDVGKLKTEALADRLHHINHTVDVRTVNEKLDSGRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGCTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWRYFDLPRNPECPVCGAAR"
BASE COUNT         4137 a         5213 c         5066 g         4775 t
ORIGIN      
        1 taaaatctaa agaaaccgtt tttcccgaca agtttctgtg ccgacaaacc tagattcccg
       61 cctgcgcggg aatgacggat tttaggtttc tgattttggt tttctgtttt tgagggaatg
      121 accgattttg ggtttctgtt tcggttttct attttgcaag aatggcaaaa tttcagattg
      181 cgggcattgt taagtatttc tattttttac ctgccgtatt tatttccgcc ccttgaagtc
      241 ggcttcttcc tcgacagaca cgctgttcat ctgtttgatc agcttttccg acttctcttc
      301 gtcttcgcag cggatgactt tcgcaatatc gctttcgagc tgtccgacat tgctgtgcag
      361 aatgatgttt ttgacgggca ggatgttgtt ggggttcatg gaaaaacggc gcagccccat
      421 acccaataaa acgcgggtaa acgcggtatc gcccgccatc tcgccgcata cggatacgtc
      481 tttgtccatg cggttggcgg tacggatgac gtgttgcagc attttcagca cggcgggatg
      541 gccgggctgg tagaggtggc tgacgctgtc gtcgccgcga tcgacggaca agatgtattg
      601 aatcaggtcg ttggtaccga cggagatgaa atcgaccagt ttcaaaatac tgccgacggt
      661 cagcgcggca gacggaattt caatcataca gccgatgccg actttaccga aggcatcgcc
      721 gcgttcggca agctggcgtt gcgcggtgtc gaggtggatg aggcactggc gcacttcgga
      781 tacggaggta atcatcggcc acatcatccg cacggggccg tgtaccgccg cacggaggat
      841 ggcgcgcatc tgggtgcgga acatgaccgg ttcggcaagg cacaggcgga tgccggtcat
      901 gcccagcgcg gggttgaggc tgccgttggg cgtgctgttt ttcccgaacc agcgcgggtt
      961 tttgtccaca cccaaatcga ctgtccgtat cgttacgctt ttgcctttca tttttttgac
     1021 aatcgcgctg tacacttcgt actgctcgtc ttcagacggc atcgtatcgc ggttcaggta
     1081 aagaaactcg ctgcggaaca gcccgatgcc gtctgcgccg aggttgtgca gcggtttcac
     1141 gtcttcggcg gattctatat tgcccacaag ctcgatgcag accccgtcag cggtggcggc
     1201 ggcggttttt ttgagcttgt tcaaatcgcg tttgtggctg cggtattcgc gggcacggcg
     1261 gcggtattcg ttcaacaccg actcatccgg cgcgataatc aacacgccgt tgataccgtc
     1321 cacaatgacc gtttcgccct cggtaatcag tttgcgcgcg ttgtgcagcc cgacgacgga
     1381 cgggatgtcc aagctcctgc ccaaaatcgc cgtatgcccg gtggggccgc cggcatcggt
     1441 aacgaaggcg gcaatgcgct gctctttaaa caaaaccgtg tcggcgggcg aaaggtcgtt
     1501 tgcaatcaga acggtttcgt caaacaggtt gtcggcaact tccaactcgt tgccctgccc
     1561 gatcaggttg ttgtggatgc ggcggacgac ttgcagcata tcctgcttgc gttcgcgcaa
     1621 ataggcatcg tccatattgt cgaattgggc ggcgagtttg tcgctctgct gcttcaatgc
     1681 ccactcggcg ttgatttttt gttcccttaa aatatcgacg ggttcgcgcg acaaggtaac
     1741 atcggtcaag agcatcaggt gcagcgagat gaacgcgccc aactcggtcg gggcgttttc
     1801 gggaatcgcg ctgcggagct gttccaactc tttgcgcgtg gctttgacgg cggcatcgaa
     1861 acgttcggct tcggcatcgg tgtccgcctc cgcaacatca tactgcggca cttcctccgt
     1921 accgcgcgca atcaggtggg cgcaaccgac ggcaatgcct ttgcccgccg ccacgccgtg
     1981 cagcacgata ctcattattc gccctcgccg aagtagccgt tgattaagtc ggtcagggcg
     2041 cgcatcgctt ccgcctcgtc cgcgccgtcg gtttccagct cgatgaccgt acccttggcg
     2101 gcggcgagca tcatcagccc cataatgctt ttgccgttga cgcggctgtc gtttttcgta
     2161 acccagactt cgcttttgaa ttgggacgcg gtttgggtga acttgttgga cgcgcgggcg
     2221 tggagtccga gtttgttgat gatttcgatg gattgtttga gcatttcggt tcccgtgtta
     2281 tgtatatcgg cagcagacgc cgtttaaaat gttttcctgc cctgccgctt cttcagacgg
     2341 catcgccgct gcgccggcac accaaatctt cgggcgcgga cgtgatggcg aaaatgcctt
     2401 ttacagccgc ctctctgaca cattcggtaa aggcggcaag gtcttccgcc gccggcgaat
     2461 attggacggc cttaaccatc atcggcgcgt tcagcccggt caaaatcgcc gatttgtttt
     2521 cgcgcacgag gcggcgggct gcattgcagg gggtcgcgcc gaaaatatcg gtcataatca
     2581 gcacgccgtc gttgtcggga aactcctgaa gcgcggcaat ggcgttgttg atgatgtcgt
     2641 cttggtcttc cgtcggctgc acgccgagta tgcggacgtt ttcaggcagc ccgcccggga
     2701 aaaaatgatg tgccagcttg cggtaggctt cgcctatggt ttcgtgtgtg atgattaaaa
     2761 gccctatcat attatgcgtc ctgttcttca ttatcctgcc ggcgtatggg cgcgatgccg
     2821 tctgaaaggc cttcagacgg catcgcgtcc ttattttccg tccaatgcgt aaatctcgcc
     2881 caaattacgc cagcagcccg ccgcatccat gccgtaaccg aaaacataac ggttcggcac
     2941 atccagtccg acataatcgg ctcggatagg cttgggtttg tcgatcagct tgttggcgaa
     3001 caccgccgca cggcagcttg ccgcacccat ttccaaaagt ttggcttgaa tggcggacat
     3061 cgtatgccct tcgtccaaaa tatcgtccag cacgacgacg tgcctgcccc ggatttgttc
     3121 cgcatcgggc atacgcttcc agttgaacgc gccgccctcc agcttgtcgc cgtaacggga
     3181 aacgtgaaca taatcaaaat ctaagggaaa acgcaacagc ggcagcaact gccccgtaaa
     3241 caccaccgcg ccgcccatca cgggcagcag cagcggatat ttgccgccca aatcgcgcgt
     3301 aatctcgtcc gccacttttt gcagtgcggc acggcattgg ccttggtcga acaaaagatc
     3361 ggcgttttca agcatcgcct gtgtttcaag gcgtttggtt tctaaatcgg tcatatgtcg
     3421 gaatcggtcg gtaaaaggaa aattataaac caaagtatcg gctgccgtct gaaccgtcct
     3481 gctcaacggt cggtacgcgc gcgcacaaac gtggcgaatt tcggcgtgcc tttctgcgta
     3541 aagccacggt aacggtaggt aatcagcgta ccgattttgg gcgggttgtc gcggtctttg
     3601 tctttgaagc cgctgccgat gcggaattcg ccgtgtcggt ttttgcagcc gaccgcgccc
     3661 aaccgtccgg cgtttcgccc tttgccctca tagtgccgcg ttaccgtgca ttcgtcgtcg
     3721 tattggcttt tcagcttcaa taattggctg ctcctgccgc cgctgtaacg ggattcgggc
     3781 tgacgcagca tcacgccttc gccgccctgc gcttcgattt gttttaaaaa gtccatcgcg
     3841 tgctgccggt cgcgcacttt gatttgcggg atgatggtaa tcggcgcgtt cggatgcgtt
     3901 ttcagccact gcgttgcgac tgccaaacgt tggtagagat tgccctgcgc cttgggtaca
     3961 tcgaaaacgt gcaggcggat gccgcgccaa tctgaagaag cagaacgcac ggcggcggaa
     4021 atctgctcga actgaccgcg tccgctatac aattcgccgt ccaaaggata aggcggaaac
     4081 tgagcggtaa aacctttggg gggcgtaaac gcatagccct gacggcttat caggtgcttt
     4141 ccgtcccaat acgcgcgcac gccgtcgagt ttctcgctca tcgcccagcc ggcaatatcc
     4201 tgccctttgt attcctgcgc cagcatcaaa tccgccgcgc ctgccgatgc agggatgaaa
     4261 accgccataa aaatcggtat gatgccgccg attgtcttct taatcacccg attcccccaa
     4321 tatccaaaca ggcggcaaac cgccataaaa caaacggcaa acccgatgcc gtctgaaaaa
     4381 ccgtttagga acacgccgat gaccctacgt tacgaaatct tccccgttac ccccttccgc
     4441 caaaactgca ccctgatttg ggacgacgaa agtggcgaag ccgtcctgac cgatgtcggc
     4501 ggcgatgtgc cgttcctgct gcaagcgttg gcaaaccgca aacttacgct cacggcaatc
     4561 tggctgacgc acggccatct cgatcacgcg ggcggcgtgg tcgaaatgtt gaaaacgcat
     4621 aaagttcccg tcctcgggcc gcatccggac gatgaattcc tgctccaatc gctgccgcaa
     4681 accaccgcgc aatacggatt tcccgtctcg cccgcctttg cgccgaaccg ttggctcgaa
     4741 gaaggcgaaa cgctcacggt cggacgctat gcctttcaag tgctgcatat tccgggccat
     4801 acgccgggac atatcgtctt ttattgtgcc gaggcggaat tgctgattgc gggcgacgtg
     4861 ctgttttacg aaaccatagg cagaaccgat tttccgcgcg gcaaccacgc cgacttaatc
     4921 aataatatcc gcaacaaatt attcgccctc cccgaaaccg tgcaagttgt cgccggacac
     4981 gggcgtatga cttccatcgg acacgaaaaa cggcacaatc cgtttttcta accgacttca
     5041 ctacggtctt cagacggcgt catctgcact gatgccgtct gaaacgcaaa aggctcagac
     5101 aaccgccgcc ttgccggtgt acctcgccgg acaaggcttt ggtaagtact tcaaacaaac
     5161 cccaaatcag aaccggtaca gttacgccgc gctttcggca ttcccgcccc tgctgaaaca
     5221 agcttttccg cacaagtcag actgcttcat cttctgccac atattccaaa gattccgaca
     5281 acgccgttgt ttcattggca cgttcggtca aatcgcgtgc aagctgcttg tttgccttca
     5341 cgcccaacag acgcagtttc tcggcgcgtc cgaccagatt cccgcgcccc tcggcaagtt
     5401 gcttaaatgc cgtctgaaag ctgttttgcg cctgatcgat gcctttgccg acgctttcga
     5461 gcgtctgtac aaagccgaca aatttgtcgt acagcttgcc gccttcgtcc gcaatcgcca
     5521 gcgcgttttg gttttgctgc tcgttgcgcc aaatattcgc caccgtccgc aaagtcgcca
     5581 gcagcgtact ggggccgacc agcataatcc gtttgtcgaa acactcttgg aacaagccag
     5641 cgtcattctg caacgccaac aggtaggccg gttcgacagg gataaacata aagacgaaat
     5701 ccaatgtgtt cacaccttcc aaatcggtgt aatccttcag cgacaagcct ttcatatgcg
     5761 cgcgtatgct ggcgacatgc gccgccaact cgcgtgccgc cgtatccgca tccgccgcct
     5821 gcgtgtagcg cacataagct gtcagcgaga ccttggagtc aatcacaatc tgcttgttgt
     5881 cgggcaggtt gaccaaaacg tcgggctgga ggcggcgcgt gccgccgtct tcctcttttc
     5941 ggacggatgc tgcctgaacc acatattccc gccctttctg aaggccggaa ttttccaaaa
     6001 ccgtttccag aatcatctcg ccccaattgc cctgaacctt attctgcgta ccggtcagcg
     6061 cgttggtcag ggcctttgcc tcgctgtgca gctgcgcatt caatccttga agccgtttca
     6121 attcgttttc caacgtcagc cgctcgcgcg attctttatc ataggtttgc ttgaccaact
     6181 cgccgaaacc ttggatgcgt tcgtttagcg ggttcaaaac ctgatggagc tgctcccggt
     6241 tctgctcggt aaaacggcgg cttttttctt ccaaaatcgt gttggcaaga ttttgaaact
     6301 gatcgctcaa acttttgcgc gcctcgccca gcaaggacag cttctcttca gaagcaaggc
     6361 gttcctgttc gatttgcgtt gccaaacgtt cgttttcaac cgccaaaccc tgtgcctttt
     6421 cctgcaactc ggtatgcgac tgcctcaacc gctccgcttc cgcctctttt tcctgcaaat
     6481 gggcaatctg tttttcggct gcggcaaaac ggttgccgac atcggcaagg tcgttttgca
     6541 cgtcgcggac agtttggcgg ctttcttcca aatcggtttc gattctttgg cggatttggc
     6601 gttccaaggc atattggtcg gcaaacgcct tccgttcctg tccgagctgc gttgccaaac
     6661 gttcgttttc aaccgccaaa ccctgtgcct tttcctgcaa ctcgatatac gactgcttca
     6721 gccgaaccga ttccgcctct ttttcctgca aatgggcaat ctgcttttcg gctgcggaaa
     6781 aacggttgcc caaagcataa ttttcgtcct gcaaatgtcg gtatttcccg tccaaaacgg
     6841 ccaattccga tgcaatttct gcgtgtgcct gttcgacaaa atcacatctt gccgcctttt
     6901 ccgccagttg cgcgttcaaa tcggcaaact cgccctgaaa cctgcccttc atcagcaacc
     6961 atgtaaacaa cacgcccgac accaacgccg ccaaaggcag caaaacagtc atcagttcca
     7021 tcaatcatcc taatattcaa acattttcac accggacaaa accgccgctt attccgattc
     7081 tacctgtttg ttcgacatag ctccaaaaaa tatagcggat tggctttaaa cctgttcgac
     7141 atcgccttac catgctgctt gcggtttcag accttttcct aattcaatat caatctgcca
     7201 caaaccctga ttaagttccc gatgtctgac atttttagaa tgatgccgtc tgaaatgttg
     7261 cagctatgtt cagacggcat acggattcag gcttttcaaa cggcaggcaa aatgaaaaaa
     7321 gggcaaaccc taaaggattt gcccttttgt tccaaacgct tagtgtacgt ctttccaata
     7381 ttctttttta atgattaatg aatatcttta gaaaattctt tatttaggaa gtaagccaaa
     7441 ggcagcataa ccgcaaatag gaaaatcatc acgacatagc ctatacgttt gcgttgcagt
     7501 tgtgcaggtt cgcccatgta cacaaggtaa ttgaccaaat cgcgtacata tgcgtcgtac
     7561 tctttttgga tcactttgcc gttaggcagg cggcggctgt gcaaaccggt agattcccaa
     7621 tacagcttag gcttcatctc gccgtgttcg tcttttacca taaccggctg acctttggca
     7681 tccaactcaa cggcttgaac accttgttgc tcccacaacg ggtggggcat accgacttta
     7741 tcgaatacag tattgttcca gccgctcgga cgggtcggat ctttatagaa gccgcgcata
     7801 taggcgtaaa ggtagtctgc acctttggaa cgcgcaatca acgtcaaatc gggcggagca
     7861 gcaccaaacc attttgccgc atctttcggg ttcatcgccg aatgcatgac atcgccgaca
     7921 ttatcggtgg taaacatcag gtttttcttg atttcttcgt cagtcaaacc gatgtctttc
     7981 agacggttga agcgcatacc gcttgcagag tggcaagaca aacagtagtt tgtaaagatt
     8041 tgcgcaccgt gctgcaggct gacttggtca cgcaggtcga tatcgacttt ttcgtagtgt
     8101 ccgccgccgc tggcgacggc tgcactcata ggcactgcca gcaataaggc agcaaaccag
     8161 tttttcagag tttgtttcat tttcgctgcc ctcatcagat attggttgca aacaagtaag
     8221 caccaacaac ggtaataccg acgtaaacaa agaacatgat tttttgtttg gcggtgctca
     8281 tggttacgcg ttcgggaacc ggtttgtttt tatccagctt ggtatagaac ggcataccca
     8341 ggaagaatgc gaaatagacg aaggacagga tgcgcgcgac caaagtacgc gtatcggttg
     8401 ccaccatcgc gcccaaaata cccaaaccga taaaggaaat gatgaacaga accaatgcgg
     8461 ttttgaagat tgggccgcga tagcggacag atttaacctc gcctttatcc aaccaaggca
     8521 gcaaggcgat cagtacaact gctgcaccca taccgattac accccatacc tgagtaccgg
     8581 caaaggaagg aatcgcacgc agaattgcgt agaacggagt gaagtaccat accggcgcaa
     8641 tgtgcggagg tgttttcagc gcattcgctg catcgaagtt tggcgcttcc aagaagtagc
     8701 cgccgccttc aggtgcaaag aacatcacgg cacagaagac aatcaagaat atcgttactg
     8761 ccaatatatc atgcacaaca taatacggaa aaaaaggtat gccatctaga gggacaccgt
     8821 tttcatcttt cagctttttg atttctacac cgtcagggtt gttggaaccc acttcatgca
     8881 aggcaatgat atgagccaca accaagccga gcaataccaa aggtaccgcg ataacgtgca
     8941 gggcgaagaa tcggttcaaa gtaacatcgg aaacgttgaa gtcaccgcgg atccaagtgg
     9001 acaaatcagg accgataaca gggatggcgg agaacaggtt aataattacc tgcgcacccc
     9061 agaaggacat ttgaccccaa ggcaacaggt aacccataaa ggcttctgcc atcaatgcca
     9121 agaaaatcag ggaaccgaaa atccacacca attcgcgcgg ttttttgtac gaaccgtaaa
     9181 tcagaccacg gaacatgtgc agataaacga cgatgaagaa gaaagatgcg ccggtagagt
     9241 gcatatagcg gataatccag ccgccggaca cgtcgcgcat gatgtactct actgcggtaa
     9301 aggcagcagg cagatggtag gcgttaaggt tgccgtccgg tttgtagttc atggtcagga
     9361 aaataccgct gacgatttga atcaccagca ccagcataga caatgagccg aagaaatacc
     9421 agaagttgaa gtttttaggc gcatagtatt cagacagatg ctctttccac attttactta
     9481 atggaaaacg ggcatctacc cagcctaaca atgcttttgc tttgctattg gtttggtttg
     9541 ccataattat cgttccttat tcttagtctt cgcccaccaa gatagttgtg tcgctcaagt
     9601 atttatatgg cgggacaacc aggttggtcg gggcaggaac acctttatat acgcggccgg
     9661 ccaagtcgaa tttcgaaccg tggcaggggc agaagaagcc gcctttccag tctgcaccca
     9721 aatcggcggg ggcaatgtcg ggacggaagg tgggcgagca gcccaaatgg gtgcagatac
     9781 cgatggcgac aaggatgttc ggcttaatcg aacgggtctc gtttttagca tactccggct
     9841 gctgttccgc atcggaattg ggatcggtaa gttcgccgtt caggcctttc aggtctttaa
     9901 gctgctgatc tgtacggttg agcacccaaa tcggtttgcc ttgccactcg gcggtcagca
     9961 gctgacccgc ttcgatttta ctgacatcca cctcgacggc agcaccggcg gccttggctt
    10021 tttccgaagg gaaaaaactg gccacaaacg gcgttgccac acccaatgct gccactccgc
    10081 ccgcgccgca ggtcgcgagt gtcaggaaac ggcggcggcc gttgttgatt tcttgattat
    10141 ccattattca gtcgtcctaa tattttggga ataccgagcc attaaacgtt gcaattttac
    10201 ccagtttgca gtgatactca aagcattatt taaaataagg taaagtttta tgatatttct
    10261 caagactcaa gccggattgt tttcgtcaaa atggcacact tccaacccga aaacctctgc
    10321 cgccgattct gccagcgcgc gtacgccgta acgttccgtc gcgtgatgcc ctgccgaaat
    10381 gaaagccgta cccgtttcat tggcaaggtg gtattgggct tcagagattt cccccgtcaa
    10441 atacagatcg acaccttcgt ctattgccgt ctgaaaaaac ccctgcgccc cgccgctgca
    10501 ccatgcaacc cgtcggattt cgcgttcggg tttgccgatg acgacaggct tacgttgcaa
    10561 aactgtttca atatgcgccg ccaatgcgcc gagtgtcttg gcttgtttca ggctgcccga
    10621 gttgagcagg ttttgttcgc cgaaccgttt ttctgtcgca aaacccaatc tgtcggcgag
    10681 ttgggcattg ttgcccagtg tgggatgtgc atccaggggc agatggtagc ctgccatatt
    10741 gatgtcgtgc cgtaacagtg cggcaatccg ttcttttttc caaccagtaa cggtcggcaa
    10801 ctcgtttttc cagaacatac cgtgatgtac caaaagcaaa tctgccttct gctccacagc
    10861 aaaatcaatc gctgccctgc ttgccgttac cgacgtaacg attttcccga tatattccct
    10921 cccttcaacc tgcaaaccgt taggggcgta atctttaaac aacgctgtct gcaatgtttc
    10981 attacaccaa gtcagaaaat ccctgcgcaa taccatcttt tttcctaatc gctttaaaca
    11041 agcgggcatt ctaatcgcaa aatgtccgga attcacattt ttccgatttg cacccgcata
    11101 tgaattattt taatatgcgc cggttcaata tgccgtctga agccccatgg attccattat
    11161 cgaactgcgc cacctcaaga ccctgctggc acttgaagaa accggcagcg tctcccttgc
    11221 cgccaaacgg gttttcctta cccaatccgc tctttcccac cagatccgta tgctcgaaaa
    11281 ccactacggc acgccgctgt tcgaacgcaa atccacgccc ttgcgcttta ccccggtggg
    11341 cgaaaggctg ctgcgcctcg cccacgaact tatacctcaa gttgctgttg cagaatggga
    11401 tttggcgcga atcacggaag gagaggcggg agagctgcgg attgccgtcg aatgccatac
    11461 ctgtttcgac tggctgatgc ccgccatggg cgaattccgc ccgatgtggc cccaagtcga
    11521 attggatatc gtatcgggat tccaagcgga tcccgtcgga ctgctgctgc aacaccgtgc
    11581 cgaccttgcc attgtttccg aagcggaaaa acaaaacgat atttctttcc aaccgctgtt
    11641 tgcctacgaa atggtcggca tttgcgcacc agaccatccg cttgccgcca aaaacgtttg
    11701 gacggcggaa gactttatcg gggaaaccct gattacttat cccgttcccg acgagatgct
    11761 ggatttgccc aaaaaaatcc tgattccgaa aaacatcaac ccgccgcgcc gacacagcga
    11821 actgaccatc gccattatcc aactggttgc cagcagacgt ggcattgccg cccttcccta
    11881 ttggacagtc atgccctacc ttgaaaaagg ctatgtcgtc caccgccaaa ttactgccga
    11941 cggattgcaa agcaaactgt atgccgccat ccgtaccgaa gatacggaca agagctatct
    12001 gaacaatttt tgccaaatca tacgcgaacg cggttttgca gatttgcccg gactgagcga
    12061 attggaaccg gtctgacccc ttattcaacc atacccggca gtttttctat tttttcatgt
    12121 atagtggatt aacaaaaacc agtacagcgt tgcctcgcct tgccgtacta tttgtactgt
    12181 ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatactgttt ttgatttttg
    12241 cccaatctgt aatctttaga ttgccaatgg gaaaccgtct actacaaata aaaaaaccct
    12301 gcgataagca gggttttttg aatttccaac attaacgttt ggagaattgt tttgcacggc
    12361 gtgctttgcg cagacccggt tttttacgtt cgacttcgcg ggcatcgcgg gtaacaaaac
    12421 cggcttgaga caaggcgggt ttcaacgcgg catcgaagtc gatcagggca cgggtaatgc
    12481 cgtggcggat tgcgccggac tggccggttt cgccgccgcc aacaacattg actttgatgt
    12541 cgaaagattc ggcgttttca gtcagaacca agggttggcg aacaaccatt cggctggttt
    12601 cgcgtgcgaa gaattcgtca acgggacggc cgtttacgat gatttgacct gtacctttaa
    12661 tcaggaatac acgagccact gaacttttgc ggcggcctgt gccgtagtag tatttaccgt
    12721 tcatgtcgcg tccttatttc agttccaaaa ctttgggttg ttgcgcagca tgggcgtgtt
    12781 ccgcacccgc atacactttc agttttttaa tcatggcgta acccaaagga cctttgggca
    12841 gcataccttt tacagcttgt tccaaagcgc ggcccgggaa ttgctcttgc atttcgcgga
    12901 aagtgcgttc gtagataccg cctgggaaac cggaatggcg gaagtatttt ttatcttcga
    12961 atttggcacc ggttacacgc agtttgtccg cattgataac aatgatgtaa tcgccggtat
    13021 cgacgtgggg ggtgtattca ggtttgtgtt tgccacgcag acggctggcg acttcggccg
    13081 caacgcgacc caagactttg tcttgggcat cgatgacgaa ccattcgcgc ttcacctcgt
    13141 ggggtttcgc tgaaaaggtt ttcatagtgg aaatccaaat agatatagaa agttgtaaat
    13201 tttaaagaca ggattcgatt ttgtcaatcg cattaccgcg ttacggaagg attttccgga
    13261 tttcggcaga ctgcatactg ctttttcggg cggggcggcg gccaatgtga aaaaccgcat
    13321 cgttgcgatg cggttttgaa tgggaatccc cgcgagagcc gtttcggccg aatccgcttg
    13381 aaccttgctg acaaggcggc tgcctcgggt agtttcgggt gcgtccgcaa aaggacgctc
    13441 gcgcccacta ctgctcccgg caaccttaag cgaacttatt ggttcaaagg aatatatgcc
    13501 ttcgcggaca ccgcagggaa aaaggggtta ttcctgcgcc aagcgggata gtgctttttg
    13561 gcaggcgttg tccatatcgg ctattttacg cgcaaaatcg ccgattgcca aatcgccgcc
    13621 gttcagggag gttttcaaca ggtcgtggac gacgttgagc gcggccataa tgacgatttt
    13681 ttcgctgtcc gcgacgcgtc cgccttcgcg gatggcttcg gctttgccgt tgagcattcc
    13741 gactgcctgc aacagtgtgt ctttttcttc tgccggcgtg ttgacggtca gccgggcgtg
    13801 catgacttcg atgtggactt gttcgatgtt catcctttaa tccttattgc tgcgtttcct
    13861 gccattgggg gaggcgcgct gccagtgcgc tgattttttc cctgctctgt tcgagcaggc
    13921 tgcggtatcg tgtattttct tctgtcaggc tgtcgatttt gttttgcagg tcttctttga
    13981 gtttgccgac ttggacgagc agggcttcgc tgagttcgtc gacggcggtt tcgtgttcga
    14041 gtttttgccg ctcgtgcgcc cgtttgagtt cggcgacggt ttctttgagg cggcggtttt
    14101 cgctgacgag ggtttcgaat ttttgtacca gcgtataaac gctgctttcg agtttttcga
    14161 tattttgttt cataacctta cctgtccgta tgccgtctga aggcctcaga cggcatctgt
    14221 ctgttgttta ttcaaaacgc gcgctgcgtt ccatcagtct ttcgacaacc tgttgcgggg
    14281 tcatttcttt gcggatgagt tgcagcaggg tttgggtaat cggcatgtcg atttggtact
    14341 tacaggcagt attgaagact tcttctatcg tgctgacccc ttcggaaacg tgtccgattt
    14401 cgaccagcac ctgatgcagt tccttgcctt ctgccaaacc caagccgacg cggcggttgc
    14461 gtgagagtgc gccggtgcag gtcaagatga ggtcgccgat gcctgccagc cccatcatgg
    14521 ttttgggctg tgcgcccatt gcggaggcaa ggcgggtgat ttcagctaat ccgcgcgtaa
    14581 ccagtgcggc acgggcgtta agcccgtact ctaggccatc ggacaatccg gtggcaatcg
    14641 ccataacatt ttttaccgcg ccgccaaccg ccacgccgat aacatcggta ctgccgtaaa
    14701 gcctcatgac ggtcgtgttg agctgcggta cgagttcttc aatccactct tggttttcgg
    14761 aggcgaggac gacggcgcag ggcagttgtt tggcgagttc ctgtgcaaaa ctcggaccgg
    14821 aaagtacgcc gattttttta ttgtcgggca atacttcttt caatacttga aaggtcagca
    14881 gccctgtatc ctgctcgaat cctttgcagg cggcgaggac ggggaggtgt cccgcgccgt
    14941 actgtttgag cagctctgcg ctgcttctca atccggcaac ggaggttacg ataaggacaa
    15001 gtccgctgtc tttgagcgcg tctgccaaat ccgcacacac ttccaaggtt tcgggaaagg
    15061 aaaagccggg cagtccgcgt ttgttttcac gcgcttcctg catttggcgg acttggtctg
    15121 cgttgcgcgt ccacagggat acgcggttgc cgtgttggga aaagtgcagg gcgagcgccg
    15181 taccccacga acctgcgccg ataacggtaa ttttcattgg tcgtctttca acatatcact
    15241 gccgttcact ttaaaacaat cggtgtttct ctgcaagtgc ggtcagggaa atgccgtctg
    15301 aaaggtgttc agacggcatt ttgccccgat gcggcacaat cagcctgtat tgcgcaaacc
    15361 ttgcgccacg ccgttgatgg tcaggtgaac catcagcagc gcgtgcggat tgtcgggttc
    15421 tttacgcagg cgtttgagca tggcgacctg caggccgttg agcgcgttca aatatggaat
    15481 cctcaaggca agcgaacggg cgaggctgcg gttgtcgcgt aaaagttctt cggtttgcag
    15541 caggtcgagc agggctttgc ggctgcgttg gtattcttcc ttaatcatgc cgaagatgac
    15601 ggcggctttt tccggcgatt cgctcaagcc ggcatagttt tccgcgaggg tgatgtcggt
    15661 tttcgccatc acttgctcca tattggaaag catggcttgg aagaacgggt tgctttgggc
    15721 gtgttcgcgc agggcggcga gcgtttcggg tttgtcttcg cacaaggttt ccaccgcgct
    15781 gccgaaaccg taccaagcag gcagcatgag gcggttttgc atccatgaga atacccacgg
    15841 aatagcgcgc aagtcctgaa tccgcgccaa ggttttgcgg ctggcaggac ggctgccgag
    15901 gttgagggtg gcgatttcct gaatcgggct ggtttgcagg aagtagtcga tgaagtcggg
    15961 gtgggtaatc agttcgcggt agtatttgaa cgatacgtcc gacaatgcct gcatcagttt
    16021 ggcatcaggg tcttttttat ccggcaggat gctggcttcc aaagtcgcgg caaccaaggt
    16081 ttccaagttg cgttgggcat tgccggggtc ggcgtatttg gcggtaatga cttcgccttg
    16141 ttcggtaatg cggatttgtc ccgccacgct gcccgccggt tgggcgagaa tggcttggta
    16201 agaagggccg ccgccgcgtc cgacgctgcc gccgcgaccg tggaacaggc gcatgcggac
    16261 atcgtatttt ttgaagagtt cgaccaagcc caattccgcc tgatacagac accatgagct
    16321 ggtaacgtag ccgccgtctt tgttggagtc ggagtagccg agcatgattt cttggatgtt
    16381 tccacggctt tcgagcaggg catcgtacca gtcgaggcgg aacatggttt ccataaccgg
    16441 acaggcgttt tcgagcgctt cgatggtttc aaacagcggc acgatattga tgcggctgcg
    16501 cggtttgccg ttttccaccg ccaacaggcc gctttctttc agcagcaatg ccaaggcgag
    16561 caggtcgctg ggttgttcgc agttggaaat aatgctttgt gtaacggcat cttcgccgaa
    16621 ttcgtctttg attttgcgcg cttcgttgaa aattgccagt tcgtggcggg tatggtcgct
    16681 gtatgtgatg aacgggctgt acagcgggcg ttgatggctc aattcgcgca acaggacggc
    16741 ttgtttttgc tcttcgttca ggctgttgta gtcttccaag cctgcgtgtt ggaaaagctc
    16801 ggtaaccaca tcggcgtgtt tgcctgcgtg ttggcgcagg tcgagcggca tcatgtggaa
    16861 gccgaacacg gacacggaac ggatgaggtc tgccaaacgg ccttcggcaa gcaggcggct
    16921 gccgttgtcg ataagggaac gttgcaattt tttcaaatca tcgagaaatt cttgtgccga
    16981 agcataaggc tcgagaaagc cgaatttgca gcccatgccc aaaccgagcg cgcgcgcttt
    17041 gcccatagcg cgcgccataa tgtaggcaat ggcgcggcgg tagggttctt ccgtgcgggc
    17101 aagttcttcg tcaggcgaga gggctgccag tgccattacg tcgtcgttga ctttgacgcg
    17161 gcggatggag agcggcaatt cgcggtagag tttgtcgagt tcgccacggt aaaaacggaa
    17221 cacggcatcg gcgtggcggc ggaaggcaaa gcgcagggtt tcggcagaaa cgaacggatt
    17281 gccgtcgcgg tcgccaccga tccagccgcc gattttgagg atgttcggaa cgcggacatc
    17341 gggataggcc gtctgaaagt cgtgttccat cttgcggtag agtttgggca gggcttcaaa
    17401 aaagctcatc gggaagatgg acacgccgtt gttgatttcg tcgttgacgc tgagtttgtg
    17461 gcggcgcgtt tcgctggtct gccacaaacc cagcaggata gtgtcgattt cgcggcgcag
    17521 ccgtgccagc gcgtcggcat tggtgcagcg ttcgcgttgc ggcagcagcg cgcggatgcg
    17581 gcggttgaaa ttcaaaacgg tttggcgttg cacttcggtc gggtgcgcgg tcaaaacggc
    17641 ggtaacggac gtattgtcca actgccgctg caccgatttg ccgtcggctt tccccgcttt
    17701 gagcctgcgg acggtttccg tcaggctgcc ttccgcgccg ccgcgtccgg cttcttcgtg
    17761 gatttggcgg cggcgttcgt ggtgcacgtc ttcggcgata ttcaaaatct gggcgaacag
    17821 gccgcaggcc aaggttaaat cgtgggtttg ttgttcgtcc aattgcggca atactttttc
    17881 aatcaatgcc gcgctgtcgt cggaagtgga caagagtttg accgtttcga caaccaatgg
    17941 cgaggcttct tcgtgcagga ggttgaacag ggattgtttc agaaattccg cgtccgccgc
    18001 caaagccgcg tcctttggat tgttcagaat atgcagttgc atgatttttc tctcattgcc
    18061 gtaaatactg taaatgtacc tcaaatgccg catccgtgcc aaaccgttca cactttaacc
    18121 gcccgtgtcc cgaaatgccg tctgaagttg aacgccgccc gacggcagcg ttacaatcgc
    18181 ccgcaactgt tttttccgaa catcatcatg accacgaccg aacacgacaa cgacgatgca
    18241 ttcctgctgc ggtacagccg gcacatcctc ttggacgaaa tcggcatcga agggcagcag
    18301 aaactttccg acgcgcatat tttggtcgtc ggatgcggcg gtttgggtgc cgccgcactg
    18361 ccctaccttg ccgcttcggg tgtcggcacg ctgaccatag ccgatttcga cacggtcgaa
    18421 ctgcacaacc tgcaacgtca ggttgcattt gacgagggcg acgttggcaa actcaaaacc
    18481 gaagccttgg cagaccgtct gcaccatatc aaccataccg tcgatgtccg caccgtcaac
    18541 gaaaaactcg acagcggccg cctgaccggt ttggttcaaa ccgccgatat cgttttagac
    18601 tgctgcgaca actatgccac gcgccaagcc gtcaaccgtg cctgcgtaca aacgaaaaca
    18661 ccgctggttt caggagcagc ggtgcgcttt gaagggcaac ttgccgtgta ccgacccgac
    18721 ttgcccgact cgccatgtta cgcctgcctg ttcgacggcg gatcggcttc agacggcatc
    18781 tgttcccttt tcggcgtatt ctcaccgctg gtcggcatca tcggctgcac gcaggcagcg
    18841 gaggcactga aaatcctgct ggatgcaggc gaaccgtcgc acggcaggct ggcggtttac
    18901 cgtgctttgg aagggggctg gcgatatttc gacctgccgc gcaaccctga atgcccggtt
    18961 tgtggcgcgg cacgataaac cctgccgccg tttcagacgg cataaaaacg gattcggcgg
    19021 aacggtttta aaaatcggcg gtgttttgac atagtgtcga taagcatact tatcttcaat
    19081 acactcaaca ggataattgt ttgcctaagc aaaaaaataa tcgccttttc cctgacgggc
    19141 gggcgaaatc cacggattgc aaaaacacaa cctgaaaatt tacatttctt t
//
LOCUS       DAWFAN010000024        18735 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWFAN010000024 DAWFAN010000000
VERSION     DAWFAN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.024"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(172..543)
                     /locus_tag="WGO65_000569"
     CDS             complement(172..543)
                     /locus_tag="WGO65_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326902.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIKIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(679..1122)
                     /locus_tag="WGO65_000570"
     CDS             complement(679..1122)
                     /locus_tag="WGO65_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326903.1"
                     /translation="MKKQITSAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1165..2034)
                     /locus_tag="WGO65_000571"
     CDS             complement(1165..2034)
                     /locus_tag="WGO65_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ0326904.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2198..3121
                     /locus_tag="WGO65_000572"
     CDS             2198..3121
                     /locus_tag="WGO65_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0326905.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     SNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(3199..3840)
                     /locus_tag="WGO65_000573"
     CDS             complement(3199..3840)
                     /locus_tag="WGO65_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0326906.1"
                     /translation="MQKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCIAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(3895..4686)
                     /locus_tag="WGO65_000574"
     CDS             complement(3895..4686)
                     /locus_tag="WGO65_000574"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ0326907.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLNVFEGRVLELPAKSHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLADLKASNPNNGKHFHL"
     gene            4852..4927
                     /locus_tag="WGO65_000575"
     tRNA            4852..4927
                     /locus_tag="WGO65_000575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4885..4887,aa:Thr,seq:tgt)
     gene            4962..5037
                     /locus_tag="WGO65_000576"
     tRNA            4962..5037
                     /locus_tag="WGO65_000576"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Thr,seq:tgt)
     gene            5179..5970
                     /gene="panB"
                     /locus_tag="WGO65_000577"
     CDS             5179..5970
                     /gene="panB"
                     /locus_tag="WGO65_000577"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ0326908.1"
                     /translation="MITVNTLQKMKAVGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            6093..6929
                     /locus_tag="WGO65_000578"
     CDS             6093..6929
                     /locus_tag="WGO65_000578"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ0326909.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7094..8932
                     /locus_tag="WGO65_000579"
     CDS             7094..8932
                     /locus_tag="WGO65_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326910.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVVKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTEEQRSRAALTAAMMYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLTDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9072..9653
                     /locus_tag="WGO65_000580"
     CDS             9072..9653
                     /locus_tag="WGO65_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ0326911.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            9663..10508
                     /gene="ispE"
                     /locus_tag="WGO65_000581"
     CDS             9663..10508
                     /gene="ispE"
                     /locus_tag="WGO65_000581"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ0326912.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKLRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHESLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PWLSV"
     gene            10516..10591
                     /locus_tag="WGO65_000582"
     tRNA            10516..10591
                     /locus_tag="WGO65_000582"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10549..10551,aa:Gln,seq:ttg)
     gene            10604..10679
                     /locus_tag="WGO65_000583"
     tRNA            10604..10679
                     /locus_tag="WGO65_000583"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10637..10639,aa:Gln,seq:ttg)
     gene            10688..10763
                     /locus_tag="WGO65_000584"
     tRNA            10688..10763
                     /locus_tag="WGO65_000584"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10721..10723,aa:Gln,seq:ttg)
     gene            10930..11913
                     /locus_tag="WGO65_000585"
     CDS             10930..11913
                     /locus_tag="WGO65_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ0326913.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            11980..12552
                     /locus_tag="WGO65_000586"
     CDS             11980..12552
                     /locus_tag="WGO65_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ0326914.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTTVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(12677..13846)
                     /locus_tag="WGO65_000587"
     CDS             complement(12677..13846)
                     /locus_tag="WGO65_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ0326915.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTLQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTITEKEIVALENVEKRSRWQRLWMRLTGQ"
     gene            13994..15520
                     /gene="ilvA"
                     /locus_tag="WGO65_000588"
     CDS             13994..15520
                     /gene="ilvA"
                     /locus_tag="WGO65_000588"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ0326916.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(15577..16650)
                     /locus_tag="WGO65_000589"
     CDS             complement(15577..16650)
                     /locus_tag="WGO65_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ0326917.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(16647..17507)
                     /gene="cysW"
                     /locus_tag="WGO65_000590"
     CDS             complement(16647..17507)
                     /gene="cysW"
                     /locus_tag="WGO65_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ0326918.1"
                     /translation="MKPYSANPNLTEPRWLRVLLTATALGFLLLMLFVPLAAVFYEAL
                     KGGWDLYLKSLSDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGRLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNTV"
     gene            complement(17762..18598)
                     /gene="cysT"
                     /locus_tag="WGO65_000591"
     CDS             complement(17762..18598)
                     /gene="cysT"
                     /locus_tag="WGO65_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ0326919.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPLKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
BASE COUNT         4433 a         4360 c         5257 g         4685 t
ORIGIN      
        1 acgccgtact aatccactat atatatccat tcatatagag aaaaattctt atacccaata
       61 taaaacaagg tctttcatta tattttgaaa aacctagaag ctttgtatct attggtttat
      121 caaaattaat aattattgca gaagacatgt aggctaataa tcttgattta tctatatttt
      181 attaattata tatttaaccc cgaagtcaat atagaaattt agtgatttta aagttctctt
      241 atcaatttta atctcaggga gtacataaaa atcctcagaa taggcaaact ctatatatgc
      301 atcatcattc gataataatt caataatatt tttcatttca tccgaaagaa atgtaaccat
      361 ctcatattga catttatttt tagataccat ttttgaatat tgattaaata attttggatt
      421 actattttta gtaattaatc cttccggagt aataaaacca ctttgtataa agtcatcact
      481 tatattaaca gccttccgaa aaaaaatata atcacactct ttgttatcaa tataaaatct
      541 catactcttt aataatgaaa tataagaatt tccagaatta caagcaattt gaaaaaacat
      601 cattactcct aaaactcata aatagtaagg aatatatata tatatgcata ttccttacta
      661 ttttttcaca aattatgctt atctatgctt atttccataa aaagcatgcc ctcgaatttt
      721 ttgacttgag ttctggaaat gagaataatt tgaatggatt tccttctgtc catgtctaat
      781 aacattttgt cttccccaag cttggtttgc taccgctctt gcttgttgag atgttcgtgc
      841 atagacatta gctcctcttg ataaagcttg tgctgcaaca cgctgcgtaa cccaacgctg
      901 cgcaaggaat ctgcccgcat gcaaaattcc tattgccacc aacggtgccg cagccccttc
      961 agtttctttc atctcctttt gagaaagctc cgccaactgc atcggcgcat ctgcctgcgt
     1021 gtggaacact tggttttcaa atgtctgatt gtccaagccg tttgccattg cgggggcaat
     1081 catagacagc atcattacgg ctgaggtgat ttgttttttc ataataactc ctttggatta
     1141 caaggttgga aaatcaaaac ctgcttaaaa tgtatgctgt acgccaaatt tcagttcgga
     1201 actgctttgc cctgaaacgt tgaaacgtgc ggatgcgttt aaagccgtgg ttttggtgaa
     1261 accgaaacct gtgccgaaat gagcgtaggt ggatgtgttt cttgcggttt cttttttgcc
     1321 gtctatgcgg tcgggctgtg tgcccagcca ttggatgcct ccggtcaggc tgattctgtc
     1381 gttggcggca aatgagatgt tggggttgag cagcaggtag ttgcccgatt tgtagcggat
     1441 gccgtctgaa agggttttgc tgccgttgat gcggtaggcg gcggtgaggg aaaggacaat
     1501 cggatctatg gctttgtagg tggtggcgcc gatgagccag gattttcccg acgaggcttt
     1561 gttgcgcgat ttttcgtaaa ccgtgctttc aagaaagctg attagggcgg ggtttttgtc
     1621 gtctttaagg aaagtgtggc tgatgccgag ggatacgtcg gacatccgtt tgttgcgggt
     1681 tttgccgttg ccgtcgagtt tgcgttcttc gtgccacaga tagctgccgc tgccgtaaat
     1741 gtcggtattc ccggtcagtc cgtagcgcaa accgagcgtg ccgacgagca tatcggtatt
     1801 gctgccgttt tcttggattt cggtcggaat ggggataaac gaggttgcgc cggtttgaat
     1861 gtaaaccggt gcggcaagtt cggcgcggtt gttttcgctg ttcaggtagg taagggaagt
     1921 ttccagtttc cattttccct tgtcggtcat tatgtcttca atcgtcaagg gcaggtcggc
     1981 ataagtggat aaaggcagga tggcgggcaa ggcgggcaaa aagatgcgct tcatatttct
     2041 cctattgata ttaattctta tttactgata ataatgaata tccgataatc ttgtctaaat
     2101 aaatttttaa atcataaaaa acaatatgtt tgtttttgtc aattatgttt cgatatgccg
     2161 tctgaaaagt ttgtgaaaaa cggttacaat ccgccgcatg aaaaaacgca ataatcctct
     2221 tccgctgttg aacggtgtca aacccagtta tttggtgctg ccgcatgaaa agcagtttta
     2281 cgggctgccg ctgctgcatt ttctgtgcat ccgctttcct tttgtgggcg cggacgattg
     2341 gcgcaggcgg ttgaacagcg gttttgtggt cggttcggat ggtgcggcgt tggacgaaca
     2401 tactttgttc gagccgggta aggtgatgtt ttattatcgg gaaaccagtc gcgagagcga
     2461 gccgcgtatt ccgtttgaag aaaagatttt gcatattgat gaacatttga ttgtggtgga
     2521 caagccgcat tttctgcccg tcatccccag cgggcgtttt ttgagggaaa cgctgctcac
     2581 acgtctgcgc ctgcgtcctg aattgcagca tttgaatgtt gaagacatta cgccgctgca
     2641 ccgcttggac aaggatacgg caggcgtgat gctgctgtcg cacaatcctg ccacgcgggg
     2701 ggcttatcag acgatgtttc aaaacaaaac ggtatggaaa acgtatgagg cgcttgcccc
     2761 gacaaggacg gatttgccgt atccgcttga tgtggtttcg cgtttggtaa ggggtgaaaa
     2821 attttttacg acgcaagagg cggaaggcga atcaaatgca catacgacgg ttgaactaat
     2881 tgaaaacagg ggggaattca gcctttaccg ccttacgccg cataccggca agaaacacca
     2941 gttgcgcgta cacatgatgg gcttgggtat gccgttattg aatgacgcgc tctatcccgt
     3001 gccgtctgaa gcgggcagcg aggattatcg gaaacctttg aagctattgg caaaaaagat
     3061 tgcgtttgca gatcctttgt cgggtcggga aagggtgttt tgcagcggat tttgcctata
     3121 atgagaggga acgcaaaccc gtatcggcgg atgccgccga tacgggtttt acattatcga
     3181 tgccgtctga aaggggtttt atccgttcag gcatacgggc gcgaagcgga ggatggtgtc
     3241 gcgcaggacg atgagtttgc cgtgaaagaa atgcgtggaa ccggcgatgg taatgacggg
     3301 caaatcttgc ggttccgccc atttcagtgc tttcccgatt tcgacgactt cgtcttccgc
     3361 gccgtgtatc atcagcgttt tggcaacatt gggaacggcg gacggctcgg ggcggccggt
     3421 atagtggcag actgccgcgc cgatgagcag caataaatcg ggtatgcgcg cttgtgcggc
     3481 aaatgtggcg acataaccgc cgaaggagaa gccggataag gcaaattcgg gggcttcggg
     3541 atgttgggcg cgggcatagt cgatgacggc gatacagtct tgcgtttcac cgcgtccgta
     3601 atcgtgtgtg ccttcgctgc cgcctacgcc gcgaaggttg ggcaggtagc agtggaagcc
     3661 gagtttgctt aaggctttgg cggcggtttg gatgactttg ttggtgttcg tcccgccttg
     3721 gagggggttg gggtggttga tgacggcaac accgcgtgcc ggtacttgtt cggacgggat
     3781 atggatggtt tctaaaatgc cggcagggcc gggtatgtgt atggtttcag gtttttgcat
     3841 aatggtttcc tcttgatttt ccgcatattc cgatgcagcc gcgaagatgg cggcttaaag
     3901 gtggaaatgc ttgccgttgt tggggttgga tgctttcagg tcggcgagca tgcggtggaa
     3961 gtccaatccg taagcgttgc tcagtttgtc ggcacgtttt ttgagtttgt ctatgttttg
     4021 ctctttgggg ctgctttgga atgccattac cgcgacattg ccgtgacttt tggcaggaag
     4081 ttccaatacg cgcccttcaa agacgtttaa caggcgttcg atgaagcgtt gatagcgttt
     4141 gtcgccgctc caccagttgg taacgaatat gccgtctgaa gagagtgcgt tgcggcagtc
     4201 tcggaagaac ggttcttcaa ccagcgtatc gatgatttgt tcgccgtcga atccgtccac
     4261 caagatgata tcggtgttgt ggcggaagac tttgatgtac tctgcaccat ctgcttcaat
     4321 aatttcaaat ttttcgccct cgaaaggcaa ctcgaacagg ttgcgggcaa tggcgataac
     4381 ctgcgggttg atatctacgg cagtttgacg cgtatcgggc aggtaggtat ctatccagcg
     4441 tgcaaacgag ccgccgccca agccgatttg ggtgatatgt tgcggaagag cgtcggtaaa
     4501 cagcagccag cccatcatcg cgcggctgta agagagcacc agctcggacg ggtggtcgag
     4561 gttcatcgag ctttgaacgg tgtcgctgcc caagtgaagc gaacggatat tgccttcttc
     4621 ggaaatgccg acttcgggaa agccggattt tgcaggacgc aggcggcggt agggatgtct
     4681 tgccatcggt gtgaacggtc ggtttgaaag gcggatattt tatcagaatg ccgatgcttg
     4741 ttctgtttca aattaatttc ttttcaaata aattacttat tcggatttgc cggggctttc
     4801 ggataaattc cttgccaagg tgcggcattg cctgcataat tcgctttctt tgccggtata
     4861 gctcagttgg tagagcacct gacttgtaat cagggggtcc cgagttcgac tcttggtgcc
     4921 ggcaccattt ctgctgatgg tgttgcaaaa catttcagta agccggtata gctcagttgg
     4981 tagagcacct gacttgtaat cagggggtcc cgagttcgac tcttggtgcc ggcaccagat
     5041 ttgacagccc gattgtgtaa aacagtcggg ctgttttgca tttgcggcac ggtcggtggc
     5101 agggctgcgg gcatttcact ataatagccc gtttcaaact gaaaaggccg tctgaaaagg
     5161 gcggggtaac aatatctgat gattactgtg aacacactgc aaaaaatgaa ggcggtaggt
     5221 gagaaaatcg cgatgctgac cgcttacgaa tccagttttg ccgcgctgat ggacgatgcc
     5281 ggcgtggaaa tgctgctggt cggcgactct ttgggtatgg cggttcaagg gcggcagtcg
     5341 acgctgcccg tcagcctgcg cgatatgtgc tatcacaccg aatgtgtagc acgcggtgca
     5401 aaaaatgcga tgattgtcag tgatttgccg tttggtgcat atcagcagag taaggagcag
     5461 gcgtttgccg ccgccgccga actgatggcg gcaggagcgc atatggttaa acttgaaggc
     5521 ggtgtgtgga tggcggaaac gactgaattt ttgcaaatgc gcggtattcc ggtttgtgcg
     5581 cacatcggtc tgaccccgca atccgtgttt gccttcggcg gatataaagt tcaggggcgc
     5641 ggcggcaagg cgcaggcgtt gcttaacgat gccaaggcgc atgacgatgc gggggcggcg
     5701 gtcgtgctga tggagtgcgt accggcggaa ctggcaaaaa aggtaactga aactgtttcc
     5761 tgtccgacca tcggcatcgg cgcgggtgcg gattgcgacg ggcaggtttt ggtgatgcac
     5821 gatatgctcg gcattttccc gggtaagacg gcgaaattcg tcaaaaactt tatgcagggg
     5881 catgatagtg ttcaagcggc ggttcgggcg tatgttgccg aagtcaaagc caaaactttc
     5941 cctgctgcgg aacatatttt tgcagattga acaaggtttg ctgccaatgc cgtctgaaag
     6001 ccgtttcaga cggcattttg ttttgccgtg cgcggcgcgt ataatcggcg cgttttgtcg
     6061 ggcaggaagc ccgaaagata aggattaccg ttatgcaaat catacatacc attcgagaac
     6121 tgcgcgcgtg gcgtaaaaat gcgggaaagg tggcatttgt gccgaccatg ggcaatctgc
     6181 atgaagggca tcttgcgctt gtgcgtgagg cgaaaaaacg cgcggacagt gtcgtggtca
     6241 gcattttcgt caaccgcctg caattcggtc agggcgagga tttcgacaaa tatccgcgta
     6301 ctttgcaaca ggatgcggac aagctggaag ccgagggtgt tgccgttgtt ttcgcgcccg
     6361 atgagaaaga actctatccg aacgtggaac agcgttacaa cgtcgaaccg cccaatctgc
     6421 aaaatgagtt gtgcggcaag tttcgtccgg gacatttccg cggggtggcg acggtggtgt
     6481 ccaagctgtt caatattgtc gcgccggatg ttgcctgttt cggcaaaaaa gactatcagc
     6541 agcttgccgt gattaaaggt ttgaccgagg atttgaattt tgatgttgaa atagtgcctg
     6601 tcgatacggg gcgcgcggaa gacggtttgg cactctccag ccgcaaccag tatttgagtg
     6661 cggcggaacg cgacgaagca ccgcgcctgt accgcgaatt aaaggctgtt gccgaatcct
     6721 tggcgcaggg cagtttggat tatgcaggtt tggaaaaacg tgccgtccaa tccctgacag
     6781 aatacggctg ggtggtcgat tatgtcgaaa tccgccgcgc cgatacgctc gaagtggcgc
     6841 gggcgggaga taagaaactg gtggtcttgg ccgccgcccg tctggggacg acgcgcctga
     6901 ttgacaattt ggaaataaaa ctcccttaaa ctgcaagcgt cgggaatgcc gtctgaagcg
     6961 gatttgcgtt tcagacagca tttatttttt tgaacggggt ttcgcaaatc tacaaacgat
     7021 tctgcttgtg ataaagttac gcctgattat atgccgtctg aaggttcgga cggctgtcgg
     7081 ataaaggaag attatgttac ccgcccgttt caccatttta tctgtgctcg cggcagccct
     7141 gcttgccggg caggcgtatg ccgccggcgc ggcggatgcg aagccgccga aggaagtcgt
     7201 aaaggttttc agaaagcagc agcgttacag cgaggaagaa atcaaaaacg aacgcgcacg
     7261 gcttgcggca gtgggcgagc gggttaatca gatatttacg ttgctgggag gggaaaccgc
     7321 cttgcaaaag gggcaggcgg gaacggctct ggcaacctat atgctgatgt tggaacgcac
     7381 aaaatccccc gaagtcgccg aacgcgcctt ggaaatggcc gtgtcgctga acgcgtttga
     7441 acaggcggaa atgatttatc agaaatggcg gcagattgag cctataccgg gtaaggcgca
     7501 aaaacgggcg gggtggctgc ggaacgtgct gagggaaaga ggaaatcagc atctggacgg
     7561 actggaagaa gtgctggctc aggcggacga aggacagaac cgcagggtgt ttttattgtt
     7621 ggcacaagcc gccgtgcaac aggacgggtt ggcgcaaaaa gcatcgaaag cggttcgccg
     7681 tgcggcgttg aaatatgaac atctgcccga agcggcggtt gccgatgtgg tgttcagcgt
     7741 acaggggcgt gaaaaggaaa aggcaatcgg agctttgcag cgtttggcga agctcgatac
     7801 ggaaatattg ccccccactt taatgacgtt gcgtctgact gcacgcaaat atcccgaaat
     7861 actcgacggc tttttcgagc agacagacac ccaaaacctt tcggccgtct ggcaggaaat
     7921 ggaaattatg aatctggttt ccctgcacag gctggatgat gcctatgcgc gtttgaacgt
     7981 gctgttggaa cgcaatccga atgcagacct gtatattcag gcagcgatat tggcggcaaa
     8041 ccgaaaagaa ggtgcttccg ttatcgacgg ctacgccgaa aaggcatacg gcagggggac
     8101 ggaggaacag cggagcaggg cggcgctaac ggcggcgatg atgtatgccg accgaaggga
     8161 ttacaccaaa gtcaggcagt ggttgaaaaa agtgtccgcg ccggaatacc tgttcgacaa
     8221 aggtgtgctg gcggctgcgg cggctgtcga gttggacggc ggcagggcgg ctttgcggca
     8281 gatcggcagg gtgcggaaac ttcccgaaca gcaggggcgg tattttacgg cagacaattt
     8341 gtccaaaata cagatgctcg ccctgtcgaa gctgcccgat aaacgggagg ctttgagggg
     8401 gttggacaag attatcgaaa aaccgcctgc cggcagtaat acagagttac aggcagaggc
     8461 attggtacag cggtcagttg tttacgatcg gcttggcaag cggaaaaaaa tgatttcaga
     8521 tcttgaaagg gcgttcaggc ttgcacccga taacgctcag attatgaata atctgggcta
     8581 cagcctgctg accgattcca aacgtttgga cgaaggcttc gccctgcttc agacggcata
     8641 ccaaatcaac ccggacgata ccgctgtcaa cgacagcata ggctgggcgt attacctgaa
     8701 aggcgacgcg gaaagcgcgc tgccgtatct gcggtattcg tttgaaaacg accccgagcc
     8761 cgaagttgcc gcccatttgg gcgaagtgtt gtgggcattg ggcgaacgcg atcaggcggt
     8821 tgacgtatgg acgcaggcgg cacaccttac gggagacaag aaaatatggc gggaaacgct
     8881 caaacgtcac ggcatcgcat tgccccaacc ttcccgcaaa ccccggaaat aatgcaggtc
     8941 catcctttca gacggcataa ggtttgccgg gaagctgcgg cattcgggca aaccgcacgc
     9001 agttcgcacg cgttttgcac ggcacgccga acccatcggc cggcaggatg gcatccgtta
     9061 aggaaattct gatgaaacac actgtatccg catcggtcat cctgcttttg accgcttgcg
     9121 cgcaattacc tcaaaataac gaaaacctgt ggcagccgtc cgaacacacc cgcagtttca
     9181 cggcggaagg gcggttggca gtgaaagcgg aagggaaagg ttcgtatgca aatttcgatt
     9241 ggacatacca accgcccgtg gaaaccatca atatcaatac ccctttgggc agtacgctcg
     9301 ggcagttgtg tcaagacagg gacggcgcat tggcagtgga cggcaaagga aatgtctatc
     9361 aggcggaaag tgcggaagaa ttaagcaggc agctggtcgg tttcaaactg ccaatccaat
     9421 atctgcatat ctgggcagat ggcaggcgtg tggcgggcgc gccttaccgc atcctgccgg
     9481 acggcatatt ggaacaatac ggttggactg tcggcagaac cgccgacagt ggggggcaag
     9541 tccgaacgtt gcaactgaat aacggaaatt tgaacatcag gctggttttc accgaaatcg
     9601 gtatgccgtc tgaaaccgaa accccggaac gctgtgcggc gcgcacgaga taaggcggac
     9661 agatgaatat tgcggacgga cggcaggcgt ttcccgcacc tgcaaaactg aatctcgatt
     9721 tgaggattac tggaaagcgg gaagacggtt atcacaatat cgaaagcata ttctgcctga
     9781 tagatttgca ggataccgta tatttgaaac taagggacga cggcaaaatc atcctgcaca
     9841 atccggttga tggaatgccg caggaagtag atttaagcta ccgtgccgca tcgttgctgc
     9901 aaaaatatgc gcgcaacccc gccggcgtgg aaatatggct ggacaaaaaa atcccgacag
     9961 gggcgggttt gggcggcgga agctcggatg cggcaaccgt tttgctggtg ttgaaccgtt
    10021 ggtggcagtg cggtctgacg cagcggcagc tcattgattc gggcgcggct ctgggggcgg
    10081 acgtaccgtt ttttattttc ggaaaaaatg cgtttgcgcg gggtataggc gacaggctgg
    10141 acgaaatgga tattccgaaa cagtggtatg tcatcgtcaa accgcccgtc cacgtttcca
    10201 ctgcaaaaat tttcacacac gaaagcttga cacgggattc tgcctcaagc ataatgccga
    10261 ctttccaaaa cctgcaaccg tttagaaatg atatgcaggc agttgtattt aaagaatacc
    10321 ctgaagtttg gaaagcctat tccgagttgt cccgatatgg atttgcctta atgacaggtt
    10381 ctggtgcgtg tgtattcacg gcgtgtcaag ataggaatag cgcatacaat atataccgac
    10441 aagtttcaga tttgtacgag gcatatttgg cagagggtct ttcaaaacat ccttggttgt
    10501 ccgtataaac attgttgggg agtcgtcaag cggttaagac actggatttt gattccagca
    10561 tgcgaaggtt cgaatccttc ctccccagcc aagtcaaacg agttggggag tcgtcaagcg
    10621 gttaagacac tggattttga ttccagcatg cgaaggttcg aatccttcct ccccagccag
    10681 ccctttttgg ggagtcgtca agcggttaag acactggatt ttgattccag catgcgaagg
    10741 ttcgaatcct tcctccccag ccagataaaa gcgtgtaagc ctgcttacac gcttttattt
    10801 catagaaata aaaatattga aatgcctttg tttgtgtcgg atgttgcagg tataatgtcg
    10861 ggcttggtac aagcagaggg aagcattgtg ttttctgagc ggaagttaaa cataaaatca
    10921 ggtgagaata tggctgcgta cgacagtttg atggtattta caggcaatgc caatcccgaa
    10981 ttggcacaac gtgttgtcag gcatttggac atttctttgg gcaatgcttc cgtatccaag
    11041 ttttcagacg gcgaagttgc cgtcgaactg ttggaaaacg tacgcgggcg cgatgttttc
    11101 atccttcagc cgacctgtgc gccgaccaat gacaacctga tggaaatcct gacgatggcg
    11161 gatgcactga agcgtgcttc ggcaggtcgt attaccacag ccattccgta tttcggctat
    11221 gcgcgccaag accgccgtcc gcgttccgtc cgcgttccga tttctgccaa actggtggca
    11281 aatatgctgt attcggcagg gatcgaccgt gttttgactg tcgatttgca tgccgaccag
    11341 attcaaggtt tcttcgatat tccagtggac aatatttatg ccaccccgat tctgttgaac
    11401 gacatcaaac aacagcggat tgaaaatctg accgtcgtca gcccggacat cggcggtgtc
    11461 gtccgcgccc gcgccgtggc aaaatccttg aatgccgact tggcaatcat cgataaacgc
    11521 cgcccgaaag ccaatgtggc agaagtcatg aacatcatcg gcgatattca aggcagaacc
    11581 tgtctgattg tggacgatat gattgacact gcaaatacgc tgtgcaaagc cgccgtcgct
    11641 ctgaaagagc ggggggcgga acgtgtcttg gcatatgcca gccacgccgt attctccgga
    11701 gaggcggtca gccgtatcgc ctcatccgaa atcgaccagg tggtcgtaac cgataccatt
    11761 cctttgtctg aagcggctaa aaactgcgac cgtatccgtc aggtaacgat tgccggtctg
    11821 ttggccgaaa ccgtccgccg catcagcaat gaagaatccg tttcatatct ctttaatgaa
    11881 gaagtgatga caggcagtat gttgctgcca taagcccgaa gccgtcttaa gctggtcgcg
    11941 gccgatgacg gcgattttac ctaacttgga gtatttaaca tgacttatga aattcaagcc
    12001 tctgttcgtg aagcacaagg cactggtgcg agccgccgcc tgcgtcgcga aggccaaatc
    12061 cccggcattc tgtacggtga aggtcaagag cctgttgcaa tcgctgtgga tcacaaaacc
    12121 gtattctacg cattggaaaa agaatctttc cataccgcgt tgattaaatt gtctctgaac
    12181 ggcgaaacca aagacgttat cgtccgtgat ttccaaatgc acccgttccg ccgcgaagtt
    12241 caacacatcg acttccaagc tgtgaaagcc gatcaacttg tacgcatccg tgttcccctg
    12301 cacatcgtta acgctgaaaa ttcccaagcg gtcaaactgc aaggcggccg tgtatctctg
    12361 ttgaacacga ctgttgaagt gcttgcattg cctgccaata tccctgcttt cttggatttg
    12421 gattgtgccg aagtggttgc cggcgacatt ctgcatctgt ccgacatcaa actgcctgaa
    12481 ggtgtagaaa gcgtttcctt gaaacgtaac gaaaatctgg ctgttgccac cgttaccggt
    12541 aaaaaacgct aattgatttc agcagcaggg cggcgcgtat gcaatacgta ccgccctgtt
    12601 gttttatgcc gtctgaaccg tgtttcagac ggcatttctt tatttgttgg aaaaacggga
    12661 tatttgaaac ggcagattac tgtcccgtca gacgcatcca aagcctttgc caccggcttc
    12721 ttttttctac attttccagt gcgacaattt ctttttcggt aatggtgtat ccgttttgcc
    12781 tgattttgat ttttcctaaa atctgtccct tttttaccgg ggcgggaatc ggctgtatgg
    12841 tttctagaat ttgttctgcc attttcgctt ccttatgtgg cagagtgatg taggcttctt
    12901 tgaggaagcc tgcgcggacg gtttttttgc tgcctccgga aatttggatt tgggcaacgg
    12961 ttttgccttt cggatatatt ttgggcgtat cgaaggcctg caatgcccag ttcagcagct
    13021 tgctgttgtc tgatgcgcgt gtttccgccg attccgaacc caatgtgatg acaaggatgt
    13081 gcctgccgtt gccggagtat gacacggcaa ggttgtagcc gccgctttct gtgtgtccgg
    13141 ctttcagacc gtttacattg ttgtccctat ataaaaggat attgcggttg ttttgctcta
    13201 tatttttgaa tttgaaagat ttgatggaaa acagcgggta atattccgga aagtcgcgca
    13261 tcaatgcttc agacagctgg gcgaggtctt tggcggtgga aacctgtcct tctctactca
    13321 agcctgtcgg gtttttgaat acagtgttct tcatgcccaa gcgtcgggct tctttgttca
    13381 tttgttgcac aaaattttca atcgagccgt tgcccagccg gccggcaagg gttagggcgg
    13441 catcgtttgc ggatagtgca atcatgcctt ttaagagttt gtcggtgctg accgtatcgc
    13501 cgggacgtac aaacattctg cttccttctg aagcccatgc ggattcgggt atttttaagt
    13561 tttcttcaga tcggatattt cccgatttca tgtttttgaa aaccagatat gcggtcatca
    13621 gttgggttag tgccgccggt tcgacagggg tattgatgtt tttggcggat aaaatctgtt
    13681 tgctttgaag gtcgataacg atgtgtgccg ctgtgagggt ttcgggtgtt tggagcgtgg
    13741 gggcggcgtg taccgccggt ttgttgggcg cgggcgatgc ggccgttgcg tgagaaacgc
    13801 ctaagatgat ggaaagcagg acgggcagga ttttatgtgc tgtcatgaaa tattctaatt
    13861 gtgtgcgtgt ttcagtctgc cgattatacg cttagggtgt ctgatcgggc ggatttttct
    13921 tgctttcgcg ccgtcttggg cgtatggttt tgggttttgc gattttaata aaccgattat
    13981 cccatattga attatgaaca cgccccttcc ttattccaat tacctcatcc gcatcctgac
    14041 ggcatctgtc tatgatgtcg cggtcgaaac gcctttggaa ccggcacgca gcctttctgt
    14101 acgtttgaaa aacaacatcc ttttgaaacg cgaagatttg cagccggttt tttcgttcaa
    14161 aatacgcggc gcgtacaaca aaatgtccaa gttgccgaaa gatgcgctcg cttgcggcgt
    14221 gattgcggca agcgcgggca atcacgcaca aggcgtggca ttgtccgcac agcgtttggg
    14281 ttgccgtgcc gttatcgtca tgccggagac tacgccgaaa atcaaagtgg atgcggtcaa
    14341 aagccgtggc ggcgaggtgg ttttgcgggg cgtttcatac aacgatgctt acgattatgc
    14401 gatggagctg gcggaacagg aagggctgac ctatatcgca ccgtttgacg atcctgatgt
    14461 tattgcagga caagggacgg taggcatgga aattgtcagc cagcatcccg atccaatccg
    14521 cgccgtattc gtaccgatag gggggggcgg tttggcggcg ggcgtggcgg catttatcaa
    14581 gcaggtccgt cccgaaatca aagttatcgg cgttcagacc aacgattcct gctgtatgaa
    14641 gcaatcggtc gaagcgggcg aaatcgtcca tttgaaagat gtcgggctgt tttcagacgg
    14701 cacggcggtc aaagtcgtcg gaaacgaaac cttccgcctc tgcaaagaac ttttggatga
    14761 aatcattaca gtcgataccg atgcggtttg cggcgcggtc aaggatattt tcgatgacac
    14821 gcgcagcatt accgagccgg cgggcgcgtt ggcgttggcg ggtctgaaag cctatatcgc
    14881 ccgagaaggc gcggaaaacc aaaccctgat tgccgttacc agcggtgcga atatgaattt
    14941 tcaccgtttg cgccatgtgt cggagcgtag tgaattgggc gagggcaacg aaggtatttt
    15001 tgccgttacc atccccgaag aacgcggcag cttccttaag tttgtcaata tattgggaaa
    15061 taggaatatt accgagttca actaccgcta cggagacgat gaaaaagcgc atatctttgt
    15121 cggacttcaa gcggcaggcc cgcaggattt ggcggttatc ggcagccggt tggatgaggc
    15181 gggattgccc aatgtcgacc tgactgatga tgagattgcc aaaatccata tccgttatat
    15241 ggttggaggg cggacggaca aagtagaaaa cgagcgtttg gtcagttttg agtttccgga
    15301 gcgtccgggg gcgttggcgc gttttttgaa ccatatgcag ggcggttgga acattacgct
    15361 cttccattac cgcaaccacg gtgcggatta cgggcggatt ttggtcggta tcgatgtgcc
    15421 gccgcacgat gccgccgcat ttgacggttt cttggaaagt ctgggataca gctatcacga
    15481 ggaaacgcaa aatgccgcgt acaagctgtt tcttgcctga cgcttgaaag cacaatgccg
    15541 tctgaaagcc tttcagacgg cattgcgctt tcatggttaa atcgaatatt caatcagttc
    15601 gttttgagag aagacataaa cctgtttcgg aatcagcttc aattctttgc cttcggcgat
    15661 tgggtaacgc gcggcatcgc tgccagccag cgtgatatgc acgtcttgtt tgccgtgttt
    15721 caccaaaata tgcgtcaatg ccccgacggc gtggactttt tcgatttcgg cacaaatcat
    15781 cggtgtttcg tgttcggcgg cgatctgcca ttcgtgcggg cggatatagc cggtggcggt
    15841 ttgttcctgc catttgtatt gtgcgtccaa tttccacgcg aagccgttat aatgccagaa
    15901 gcctttttcg atgcgtcctt caaaagcgtc ggtttcgccg aggaactcgg taacgaaggc
    15961 attttcgggt ttgcggtaaa tagcttcggc gctgccggtt tgttcgattt tgccgtggtt
    16021 catcacgacg atttcgtcgg aaacttcgag ggcttcttct tggtcgtgcg taaccagaat
    16081 gctggttaca cccaggttgt gatggatatc gcgcagccag gtgcgtaatt ctttgcgtac
    16141 tttggcgtcc aacgcgccga agggttcgtc caaaagcagg agtttcggtt cgaccgcaag
    16201 cgcacgggcg agggcgatgc gctggcgttg cccgccggag agttggtgcg gataggattt
    16261 tgccaaatga gagagctgca cgagcttgag caattcttcg actttggcgc ggatttgtcc
    16321 tttggacggg cgttcggact tgggcaatac ggtcaaaccg aaagcgacgt tgtcaaacac
    16381 gttcatgtgg cggaagaggg cgtagtgttg gaacacgaag ccgactttgc gctcgcgcac
    16441 gtgtttggcg gttacgtctt gcccgtcaaa gaggatttgg ccgccgtcgg cgttttccag
    16501 tccggcgata atgcgtaaaa gcgtggtttt gccgcagccg gacgggccga gtagggaaac
    16561 gagtttgccg gtggggacgt tgaggttgat gtttttcagc gcgtgaaaat tgccgaagtg
    16621 tttgtttaag ttttggatgg tgatactcat actgtgttcc tttcggcggc ggcgagtttt
    16681 ttgtcttgta atttggtaat gatgttttgc accgccagcg tcgccagtgc taaaagtgcc
    16741 aatacgccgg agagggcgaa tgcgccggta aagttgtatt cgttgtagaa gatttcgacc
    16801 aaaagcggga cggtgttggt ttcgccgcgt atgtgtcccg ataccacgct gaccgcgccg
    16861 aattcgccca tcgcgcgggc gttggtgagg atgatgccgt agagtaacgc ccatttgatg
    16921 ttgggcaggg taacgcgcca aaacatctgc cagccgcttg cgccgagtat cagcgcggcc
    16981 tgttcttcgc tgtcgccctg cgcctgcatt aaaggaatga tttcacgcgc gacaaagggg
    17041 aaggtaacga acaaggtcgc caaaataatg ccggggatgg caaagataat ctgtatgcct
    17101 tgcgcttcga gccggccacc caatgccgtg tgcgcgccga acaataagac gaacatcaaa
    17161 ccggctacca ctggcgatac ggaaaacggc aaatcgagca gggtggtcag caactgcttg
    17221 cctcggaagt caaaacgggt cagcagccac gccatcgcca cgcccaagac ggcattgacg
    17281 gggacgacaa tcagcgcagt aatcagcgtc aatttgatgg cagaccacgc ttcgggatcg
    17341 cttaaggatt tcaggtacaa atcccaaccg ccttttaagg cctcgtaaaa cacggcggcg
    17401 agcggcacga acagcatcag caacagaaag cccaaggcgg tagcagtcag caatacgcgc
    17461 agccaacgcg gttcggtcag gttgggattg gcggaatagg gtttcatggc ggtggtgttc
    17521 cccggttggt tgttttaagg tgttcggatg ccgtctgaaa aaccgttttt tgtggggcgg
    17581 attcgttttc agacaacctt ttattgatta agggaataaa aacgtctagc taccgtcatt
    17641 cccgcgcagg cgggaatcca ccacagggca acaggaaaac agaaaataaa taaggcagcc
    17701 gaaattcacc aatggattcc cgcctgcgcg ggaataacgg taacaggtat ttcagacgac
    17761 ctcaaccctt cgcgcccgaa cgcctgccca acgcccactg catcacgttc agcgcaaaca
    17821 gaatcacaaa cgaaaccagc agcataaaca acgccaccgc cgacgcgccc tgtacgtcga
    17881 actgttccag cttgcccgta ataatcagcg gcaggatttc agaaaccatc ggaatattgc
    17941 ccgcgataaa aatcaccgag ccgtattccc ccgttgcccg cgcgaacatc attcccgcgc
    18001 cagtcaagag tgccggcgtg atttccggca gcagcacgcg gcggaacgtc gtaaagcggt
    18061 ttgcgcccaa agtcgccgcc gcttcctcat attcgcccga taattcctcc aacaccggct
    18121 gcacggcgcg gacgataaag ggcaggctga cgacgaccag cgcaatccaa atgccgacgg
    18181 gtgtaaacgc gattttgatg cccaaaggct cgaaaaaacg gcctatccaa ccgttgggcg
    18241 catacagggt cgccaacgcg atacccgtaa ccgccgtcgg cagcgcaaac ggcaaatcga
    18301 ccagcgcgtt cgccagaccc ttgagcggaa attcatagcg caccaacacc cacgccacca
    18361 gcgtgccgaa cacgacattg gtcagcatcg cataaaacga catccgcaaa ctcagccaca
    18421 ccgccgccaa cacgttcggc tcggcaatcg tgttccaaaa gccgccccag ccgatttccg
    18481 ccgccttcgc cgccatcatc gcaaacggca agaccacaag cagcgacagg cacaatacgg
    18541 tcagcccgag gctgagtttg aagccgggca gtacgccggg cgttttgagc ggtaacataa
    18601 aacaatgctg aaaataagga aaaggaagga ctactttaac gatgccgtct gaaaaacgga
    18661 aagaatggaa agtttggtgc aaagacgaat ttgttataaa gcggttggca gtttctcaag
    18721 cgggcgcgat gtttt
//
LOCUS       DAWFAN010000025        18191 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWFAN010000025 DAWFAN010000000
VERSION     DAWFAN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.025"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(135..470)
                     /locus_tag="WGO65_000592"
     CDS             complement(135..470)
                     /locus_tag="WGO65_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0326920.1"
                     /translation="MFKDWKEHTALVKKSFGELGKSHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            599..1504
                     /gene="mtrA"
                     /locus_tag="WGO65_000593"
     CDS             599..1504
                     /gene="mtrA"
                     /locus_tag="WGO65_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ0326921.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1566..2054)
                     /locus_tag="WGO65_000594"
     CDS             complement(1566..2054)
                     /locus_tag="WGO65_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0326922.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2201..3103
                     /locus_tag="WGO65_000595"
     CDS             2201..3103
                     /locus_tag="WGO65_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0326923.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLAEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3144..4274)
                     /locus_tag="WGO65_000596"
     CDS             complement(3144..4274)
                     /locus_tag="WGO65_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0326924.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4479..7103
                     /gene="alaS"
                     /locus_tag="WGO65_000597"
     CDS             4479..7103
                     /gene="alaS"
                     /locus_tag="WGO65_000597"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ0326925.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALVKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGEEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSEQA
                     IVLLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLASVESWLCQKLS"
     gene            7165..7896
                     /locus_tag="WGO65_000598"
     CDS             7165..7896
                     /locus_tag="WGO65_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326926.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8205..11018
                     /locus_tag="WGO65_000599"
     CDS             8205..11018
                     /locus_tag="WGO65_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0326927.1"
                     /translation="MKTLLQLQTAAQNFAAYYKDQTDERREKDTFWNEFFAIFGIDRK
                     NVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTKAKEQAERYLQEIGRTKPS
                     ALPEYYAVSDFAHFHLYRRVPEEGAENQWQFPLEELPEYITRGVFDFMFGIEAKVRQI
                     QEEANIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFLFFADDSAVFRRNYLFQDF
                     LENCKEADTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTA
                     KQHRALIDCGNFDWRNISPEIFGTLFQSVMDAQERREAGAHYTEAANIDKVINGLFLE
                     NLRAEFEAVKALKRDKAKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDII
                     AEALKDKAGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDG
                     EVACHTLPLEDAAEIIHANSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHI
                     KGYGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLKQGIEIHF
                     AHRTFQWTSQAAGKAAVHCIIVGFRQKPQMPSEKNLYDYPDIKGEPVKHTAANINPYL
                     IDAPDLIITKRRSQISGEIEMLYGNKPTDGGNLILSTAEKDALIAAEPLAEQYTRPFV
                     GAEEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKAMREASSKAATQKKAA
                     TPWLFDEIRQPSEGNYLIIPRVSSESRRFVPIDYLSHQIIISDSSFMLPNATLYHFGI
                     LSSTMHNAFMRTVAGRLKSDYRYSNTVVYNNFPFPESCRMPSENDCSDPLRAAVEAAA
                     QAVLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGK
                     NTDDEAERTAFLFELYRKAAAIA"
     gene            complement(11820..12503)
                     /locus_tag="WGO65_000600"
     CDS             complement(11820..12503)
                     /locus_tag="WGO65_000600"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ0326928.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDKDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            12800..15103
                     /gene="parC"
                     /locus_tag="WGO65_000601"
     CDS             12800..15103
                     /gene="parC"
                     /locus_tag="WGO65_000601"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ0326929.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESLTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            15124..16641
                     /locus_tag="WGO65_000602"
     CDS             15124..16641
                     /locus_tag="WGO65_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ0326930.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQALKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
                     TASATFILVAASYLSAIFTSLSVRYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAV
                     VVINTEGLAVLFNRKAKDLFPALEIGRRADLSDSAAELWDQASPHTFEYVLGTLGLTA
                     RIRAVPMNKEPDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAAGCIRLDMQGNHPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            16693..17160
                     /locus_tag="WGO65_000603"
     CDS             16693..17160
                     /locus_tag="WGO65_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0326931.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            17264..18016
                     /locus_tag="WGO65_000604"
     CDS             17264..18016
                     /locus_tag="WGO65_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0326932.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         4559 a         5257 c         4392 g         3983 t
ORIGIN      
        1 ttcacgctta ccccttgttt ctcaaatgcc gtctgaaaaa aacggctgta tatgttgtct
       61 gcaaaatatc gggaagcata acagacaaaa tgccgtctga aatattttca gacggcattt
      121 caatccgatt cggattattt ttgcgtttca accgcttcca atgcacgcag ggcgtaagtg
      181 taagcggcac cggcattcag ggcgatggcg gttgccaatg cgccggcgat ttcgctgtcg
      241 gtcgcaccgg ctttggcggc ggctgcggcg tgaacgctga tacagctttc gcaacgggtg
      301 gtaatggcaa cggcgatggc aatcagttcg cgcgttttgg catccaatgc ttcggcggcg
      361 gcggcctgtt ccaatgcgcc gtatgcctgc agcattttag gatgggattt acccagctcg
      421 ccgaacgatt ttttaaccaa tgcggtatgt tctttccaat ctttaaacat tttcttttcc
      481 tttctcttgc gtttaaccct gatacgcgct tgcgtatctg ttttcgatgt gcgtattatt
      541 gcaattattc agttgtgttt ctcgtttaat catctcattt catggttcaa aaagatttat
      601 ggacattctg gacaaactgg tcgatttcgc ccaattgacg ggcagtgtgg atgtgcagtg
      661 ccttttgggc ggacaatggt cggtacggca tgaaaccttg caacgcgaag gactggtaca
      721 cattgttaca tcgggcagcg gctatctctg catcgacggc gaaacttccc cgcgtccggt
      781 cagtacaggg gatattgtat ttttcccgcg cggcttgggt catgtgttga gccacgacgg
      841 aaaatgcgga gaaagtttac aaccggatat gcggcagcac ggtgcgttta cggtcaagca
      901 gtgcggcaac ggacaggata tgagcctgtt ttgcgcccgt ttccgctacg acacccacgc
      961 cgatttgatg aacgggctgc ctgaaaccgt ttttctgaac attgcccatc cgagtttaca
     1021 gtatgtggtt tcaatgctgc aactggaaag caaaaaacct ttgacgggga cggtttccat
     1081 ggtcaacgca ttgtcgtccg tcctgctggt gcttatcctg cgcgcctatc tcgaacagga
     1141 taaggatgtc gaactctcgg gcgtattgaa aggttggcag gacaaacgtt tgggacattt
     1201 aatccaaaag gtgatagaca aaccggaaga cgaatggaat gtcgacaaaa tggtggcggc
     1261 tgccaatatg tcgcgcgcgc aactgatgcg ccgtttcaaa agccgggtcg gactcagccc
     1321 gcacgccttt gtgaaccata tccgcctgca aaaaggcgcg ttgctgctga aaaaaaaccc
     1381 agattcggtt ttgtcggtcg cactgtcggt aggctttcaa tcggaaacgc acttcggcaa
     1441 ggcgttcaaa cggcaatatc acgtttcgcc gggtcaatac cggaaagaag gcgggcaaaa
     1501 ataaatcggg gcttcaaacg caaatgccgt ctgaaaaggc tttcatacgg catttgcgta
     1561 ccgcgtcatt tcaagggctg catcttcatc acttccatca aaaagttggt aaatgcgggg
     1621 ttgttgggtt tgacatccat atttttccaa cgctgctgcc agccgcgcaa ggcattctgg
     1681 atatacagct tggactgttc cgtattgatt gcgccccgct ggctgtctat cgccgaacgc
     1741 aggtagattt catacatact gtcatcgacg gcattgcgtc cgaccaggcg ttttctgaag
     1801 ttgttcagat attgcgccgc ctgaaccttg gtcattttac cgatacccac ctgatagccc
     1861 aagcgcgtcg cttcatcgct gattttggca acatccgtcc aatgcgaaga ggcaaggcgg
     1921 aaaccttttg caggtgcttc cgttttgacg gtattgatag gattcacggg gatttccgtc
     1981 aatgtgggca cataaataga ctggcagccg gaaagaactg ccacaatgga aagagggata
     2041 aggtattttt tcatgccccc attataatca agtttgcctt gagaaaacaa attgttcggc
     2101 aagaaaaata aaatttcggc atcagaagca ggcaaaaaca cattccacaa gccttgccgc
     2161 aaggtttaca atccgaccgt ccttatcgca acgaccgttt atggataccg caaaaaaaga
     2221 cattttagga tcgggctgga tgctggtggc ggcggcctgc tttaccatta tgaacgtatt
     2281 gattaaagag gcatcggcaa aatttgccct cggcagcggc gaattggtct tttggcgcat
     2341 gctgttttca accgttgcgc tcggggctgc cgccgtattg cgtcgggaca ccttccgcac
     2401 gccccattgg aaaaaccact taaaccgcag tatggtcggg acgggggcga tgctgctgct
     2461 gttttacgcg gtaacgcatc tgcctttggc caccggcgtt accctgagtt acacctcgtc
     2521 gatttttttg gcggtatttt ccttcctgat tttgaaagaa cggatttccg tttacacgca
     2581 ggcggtgctg ctccttggtt ttgccggcgt ggtattgctg cttaatccct cgttccgcag
     2641 cggtcaggaa acggcggcac tcgccgggct ggcgggcggc gcgatgtccg gctgggcgta
     2701 tttgaaagtg cgcgaactgt ctttggcggg cgaacccggc tggcgcgtcg tgttttacct
     2761 ttccgtgaca ggtgtggcga tgtcgtcggt ttgggcgacg ctgaccggct ggcacaccct
     2821 gtcctttcca tcggcagttt atctgtcgtg catcggcgtg tccgcgctga ttgcccaact
     2881 gtcgatgacg cgcgcctaca aagtcggcga caaattcacg gttgcctcgc tttcctatat
     2941 gaccgtcgtt ttttccgctc tgtctgccgc attttttctg gccgaagagc ttttctggca
     3001 ggaaatactc ggtatgtgca tcatcatcct cagcggcatt ttgagcagca tccgccccac
     3061 tgccttcaaa cagcggctgc aatccctgtt ccgccaaaga taaaaaatgc cgtccgaaca
     3121 tccttcagac ggcatatcgg gctttatttc cccgccttca catcctgcca ctggcgcacc
     3181 ataaacttca atgccgccgg ctggataggc accatgataa agctgttttt caaatcctcc
     3241 tcggttggga aaatcgtatt gtcgttttta aattcgtctt ccatcagctc acgcgccggc
     3301 ttgctcgaag gcgcataagt aacgaaattg ccgtttttcg ccgacacttc cgggtcgagg
     3361 aagtcgttga tgtatttgtg cgcgttggcg acgtttttcg catctttcgg aatcacgaaa
     3421 gaatccaccc aaatccccac gccctctttg ggcatcatca cgcggatttt ttccttgccg
     3481 cccgcttctt cggcacggcg tttggcgatg ttcaaatcgc cgccgaaacc gattgttacg
     3541 caggtatcgc cgcgcgccaa atcatcgata aagccggacg aagtaaagcg tttgatattg
     3601 gggcggtttt tcttgagcag ggcggttgcc tccctgatgt cttccgtatt gctgctgttc
     3661 gggtttttac ccaaatagtt caacaccata ggatagattt ccgccgcgct gtctaaataa
     3721 ctgataccgc attgtttgag tttggacgtg tattcggggt cgaacaccaa atcccactgg
     3781 ttgtccggca gcttgtccgt acccaaagcc tttttcacgc gttcggtatt gatggcgaag
     3841 gtatttgtcc cccaataaaa cggcacggcg tattcgtggc cgggatcgac cccgtccatc
     3901 agcctcatca tttcgggatt gaggtgttta taattgggaa tcagcgattt atcgattttc
     3961 tgatacgcac ctgccttaat ctgcctgccc acaaacgcat tggacggcgc gacaatgtcg
     4021 taaccggatt ttccggtcag caccttgctt tccagcgttt catcgctgtc gtacacatca
     4081 taagtaacct tgatgccgtt tttcttttca aaatcggcaa cggtttccgg atcgacgtat
     4141 tccgaccagt tgtaaatttt caatacgttt cggttttccg ccggcgccgg tttttcggca
     4201 ggcggtttgt ccgaaccgcc gcacgctgca agcagcaaag cagtcaggac ggcgagtggc
     4261 agatgtttgg ccattatcat tccttgcata tcgggttgga gaaagcggtc attatagccg
     4321 atattggcaa cagggcttca gacggcattc aaaatcccgc cacacccttc cgaaaaccgc
     4381 cgcttccata gctagaaaca gggatttgcg gtaagatacc gccgttcgtt ttccctgctt
     4441 ttaccatgac aagacatttg agagacattg aaaaaattat gaaaacctcc gaactgcgcc
     4501 aaaaattcct aaaatttttt gaaaccaaag gccacaccgt cgtccgctct tccagcctcg
     4561 tgccgcacga cgacccgacc ctgttgttta ccaacgcagg catgaaccag tttaaagacg
     4621 tatttttagg tttcgacaaa cgcccgtaca gccgcgccac caccgcgcaa aaatgcgtac
     4681 gcgcaggcgg caagcacaac gacctggaaa acgtcggcta caccgcccgc caccacacct
     4741 tctttgaaat gatgggcaac ttctccttcg gcgactactt caaacgcgac gccatccact
     4801 ttgcttggga atttctgact tcccccgaat ggctcaacat ccctaaagac aaactgttgg
     4861 cgaccgttta cgcggaagac gacgaagcct acaacatctg gttgaacgaa atcggtatgc
     4921 cgtccgagcg catcgtccgc atcggcgaca acaaaggcgc gaaatacgcg tccgacaact
     4981 tctggcaaat gggcgacacc ggcccttgcg gcccctgctc cgaaattttc tacgaccacg
     5041 gcgaagaaat ttggggcggc attcccggca gccccgaaga agacggcgac cgctggatcg
     5101 aaatttggaa ctgcgtattc atgcagttca accgcgacga acaaggcaat atgaatccgc
     5161 tgcccaagcc gtccgtcgat accggcatgg gcttggaacg catggccgcc gtcatgcagc
     5221 atgttcacag caactacgaa atcgacttgt tccaagacct gctcaaagcc gttgcccgcg
     5281 aaaccggcgc gccgttcagt atggaagaac ccagcctgaa agtcatcgcc gaccacatcc
     5341 gctcctgctc gttcctgatt gcagacggcg tcttgccttc caacgaaggc cgcggctacg
     5401 tattgcgccg cattatccgc cgcgccgtgc gccacggtta caaactgggt caaagcaaac
     5461 cgttcttcca caaactcgtt gccgatttgg tcaaagagat gggcggtgcc taccctgaat
     5521 tgaaagaaaa acaagcccaa atcgaagaag cattgaaaaa cgaagaaagc cgttttgccc
     5581 aaacgctgga aaccggtatg gctttgttgg aaaacgcgct ggtcaaaggc ggcaaaacac
     5641 tcggcggcga aatcatcttc aaactctacg atacctacgg tttcccatac gacttgactg
     5701 ccgacatctg ccgcgaacgc aatatcgaac cggacgaagc aggcttcgag cgcgaaatgg
     5761 aagcccaacg cgcacgcgca cgcgccgccc aaagcttcaa agccaacgcc caactgcctt
     5821 atgacggtca agacaccgag tttaaaggtt atagcgaacg ccaaaccgaa tccaaagtcc
     5881 tcgccctcta caaagacggc gagcaagtca acgaattgaa cgaaggcgac agcggcgcag
     5941 tcgtcatcga ctttaccccg ttctatgcag aatccggcgg ccaagtcggc gatgtcggct
     6001 atatcttctc aggcgaaaac cgctttgaag tacgcgatac ccaaaaaatc aaagcggccg
     6061 tattcggtca attcggcgta caaacttcag gccgtctgaa agtcggcgac agcgttaccg
     6121 ccaaagtgga tgacgaaatc cgcaatgcca atatgcgcaa ccacagcgcg acccacctga
     6181 tgcacaaagc cctgcgcgac gtattgggcg gacacgtcga acaaaaaggc tctttggtta
     6241 ccgccgaatc cacccgtttc gacatttccc atcctcaagc ggtcactgcc gaagaaatcg
     6301 ccgaagtcga acgccgcgtc aacgaagccg ttttggcgaa cgttgccgtc aatgcagcca
     6361 ttatgagcat ggaagacgcg caaaaaaccg gcgcgatgat gctcttcggc gaaaaatacg
     6421 gcgaagaagt gcgcgtactg caaatgggcg gtttctctac cgaattgtgc ggcggcacac
     6481 acgtttcacg caccggcgac atcggcctct tcaaaatcat cagcgaaggc ggtattgccg
     6541 caggcgtgcg ccgtatcgaa gccatcaccg gcctgaacgc actcaaatgg gcgcaagagc
     6601 aagagcgttt ggtgaaagac attattgccg aaaccaaagc ccaaaccgaa aaagacgtac
     6661 tggcaaaaat ccaagcaggc gcggcacacg ccaaagcatt ggaaaaagaa ttggcacgcg
     6721 ccaaagccga actcgccgtc cacgcaggcg ccaaactctt ggacgatgca aaagacttgg
     6781 gcgcagccaa actcgttgcc gcccaaatcg aagccgacgc agccgccctg cgcgaaatcg
     6841 taaccgacct gaccggcaaa tcagaacaag ccatcgtatt gctcgccgca gtcaacgagg
     6901 gcaaagtttc cctgtgcgca ggcgtatcca aagccctgac cggcaaagtg aaagcaggcg
     6961 atctggtcaa atttgcagcc gaacaagtcg gcggcaaagg aggcggccgt cccgacttgg
     7021 cgcaggccgg cggcacggat gccggcaaat tgcctgaaat gctggcaagc gtggaaagct
     7081 ggctttgtca gaaattaagt taaatttaaa tcttaataag ccatttgagt cttgttcaaa
     7141 tggcttatat tttggattgt tagaatgaat actaaaacag agttacaaaa acttttagaa
     7201 gaggatatat caactttaac agaaacacta atttgtgcag atgctcttcc tccaagatat
     7261 gtacgtagca ttgcaactcc tattgtccgt cgatggttaa ttgacaaaca attaaatatt
     7321 ctcgcaaaag aaataggttt aactatagaa ttgcccatcc ttgatacaag tttggtattt
     7381 gaaaaattgt caaccttaga gaataaagtt aatttttata tggctggtgg agtttattta
     7441 ggcggagaat ttattagctc tatatatcat tcatcacaag aattttctgg tgaaccaata
     7501 atctatgctg aaccaaatat aattttatgt cctgctgaaa aatttttaac tttaaaacga
     7561 gttttccata atggaaatat ttttaatatg aatcagataa ttactttttt atccaataaa
     7621 caaggaggag tacattttga taaaaattac gataaatata aaacttggca agttgctata
     7681 gaaaaagcag caaatttttt aaaactcggt aatccatata atgaagataa attatctctt
     7741 agtgaagaac atgatactat tttagtagtt ttaccattag aaaaaggata tgaatggaat
     7801 tgtcttgaga tagaagtact atccgctgct caatctctag caaatatcta ttgcaacaaa
     7861 gttcgcctca tagatggtca tgtttggaag gaataaaaga aaaagcatag ttggtaattt
     7921 ttagttacgt agggtgggct tcagcccacc gttttttcat ttttgattgt tgattggtgg
     7981 gctaaagccc accctacggt tactaaaatc ccgtcattac ctcgcaggcg ggaatccggt
     8041 ttgttcggtt tcggtttttt agggacagtg ccgcaacatt aaatttccag attcccactt
     8101 tcgcacaaat aacagcgcaa atagggcaaa gataaggata tggggggttt tttctttgaa
     8161 tttactatac aattcgcccg tccccgttat agaaacccaa gccgatgaaa accctgctcc
     8221 aactccaaac cgccgcacaa aacttcgccg cctactacaa agaccaaacc gacgagcgcc
     8281 gcgagaaaga caccttctgg aacgaatttt tcgccatttt cggcatcgac cgcaaaaacg
     8341 tcgcccactt cgaatacccc gtcaaagacc ctgccgacaa cacccaattc gtcgatatat
     8401 tttgggaagg catcttcctt gccgaacaca aatccgccaa caaaaacctg accaaggcca
     8461 aagagcaggc ggagcgttat ttgcaggaaa tcgggcgcac caagccctcc gcgctgcccg
     8521 aatattacgc cgtcagcgat tttgcccatt tccaccttta ccgccgcgta cctgaagaag
     8581 gcgcggaaaa ccaatggcag ttcccattgg aagaattgcc cgaatacatc acgcgaggcg
     8641 ttttcgactt catgttcggc atcgaagcca aagtccgcca aattcaagaa gaagccaaca
     8701 ttcaagcggc ggcggccatc ggcaggctgc acgacgcgct caaagaagaa ggcatttacg
     8761 aagaacacga gctgcgcctc ttcatcacgc gcctgctttt cctctttttt gccgacgaca
     8821 gcgccgtttt ccggcgcaac taccttttcc aagacttttt agaaaactgc aaagaagccg
     8881 acacgctcgg cgacaagctc aaccaactct ttgaatttct caacacaccc gaccaaaagc
     8941 gcagcaagac ccaaagcgaa aaatttaaag gtttcgaata cgtcaacggc ggcctgttta
     9001 aagaacgcct gcgcaccttc gacttcaccg ccaagcagca ccgcgcctta atcgactgcg
     9061 gcaatttcga ctggcgcaac atcagccccg aaatcttcgg cacgctcttc caatccgtca
     9121 tggacgcgca agagcggcgc gaagcaggcg ctcactacac cgaagccgcc aacatcgaca
     9181 aagtcatcaa cggccttttt ttagaaaacc tgcgcgccga atttgaagcc gtcaaagccc
     9241 tcaaacgcga caaagccaaa aaactcgccg ccttctacca aaaaatccaa aacctgcaat
     9301 tcctcgaccc tgcctgcggc tgcggcaact tcctgatcgt tgcctacgac cgcatccgcg
     9361 cccttgaaga cgacatcatc gccgaagcgc tcaaagacaa agcaggcggc ctgttcgaca
     9421 gcccgtccgt ccaatgccgt ctgaaacagt ttcacggcat cgaaatagac gaatttgccg
     9481 tcctcatcgc ccgcaccgcc atgtggctca aaaaccacca atgcaacatc cgcacgcaaa
     9541 tccgcttcga cggcgaagtc gcctgccata cgctgccgct cgaagacgcc gccgaaatca
     9601 tccacgccaa cagcctccgc acaccttggc aggcggcgga ctacatcttc ggcaatccgc
     9661 cctttatcgg ctcgacctac caaaccaaag agcagaaaaa cgacctcgaa agcatctgcg
     9721 gccatatcaa aggctacggc ctgttggatt acgtctgcaa ctggtatgtc aaagccgccg
     9781 gcatcatggc gcagcatccc caagttcaga cggcatttgt ttccaccaat tccatctgcc
     9841 aaggccagca ggtcgaaatc ctttggggca gccttttgaa gcaaggcatc gaaatccact
     9901 ttgcccaccg caccttccaa tggacgagcc aagccgcagg caaagccgcc gtccactgca
     9961 tcatcgttgg cttccgccaa aagccgcaaa tgccgtctga aaaaaacctc tacgactatc
    10021 ccgacatcaa aggcgaaccc gtaaaacaca ccgcggccaa catcaatccg tatctgattg
    10081 acgcgcccga tttgattatt accaagcgca gaagccaaat cagcggggaa attgaaatgc
    10141 tttatggtaa taagccaacc gacggcggca accttatcct ctcaaccgcc gaaaaagacg
    10201 ccctgattgc cgccgaaccc ttggcggaac aatacacccg cccctttgtc ggcgcagagg
    10261 aatttatcaa cggcaaaacc cgttggtgcc tgtggtttca cggcgtatcc gatgtcaaac
    10321 gcaaccacga cctgaaacaa atgccccaag tccaagctcg tattcaggcg gtcaaagcca
    10381 tgcgcgaagc cagcagcaaa gccgccacgc aaaaaaaagc agcgaccccg tggctcttcg
    10441 acgaaatccg tcaaccttca gagggcaatt atctaattat tccacgcgtc tcatccgaaa
    10501 gccgccgttt cgtccccatc gattatctgt cgcatcaaat catcatcagc gattcatcgt
    10561 ttatgctgcc aaacgccacc ctctaccact tcggcatcct cagctccacc atgcacaacg
    10621 cctttatgcg tactgttgcc ggccgtctga aaagcgatta tcgctattcc aataccgtcg
    10681 tgtacaacaa cttccccttc cccgaaagct gccggatgcc gtctgaaaac gactgctccg
    10741 acccgctccg cgccgccgtc gaagccgccg cccaagccgt cctcgacgcg cgcggacaat
    10801 accgccgaga agcgcgggaa gccggtttgc ccgagccgac cctcgccgaa ctctatgcgc
    10861 ccgacgcagg ctataccgcc ctagacaaag cccacgccgc cctcgacaag gcagtcgata
    10921 aagcctacgg ctacaaaaca ggcaaaaaca ccgacgacga agccgaacgc accgccttcc
    10981 tgttcgagct gtaccgcaag gcggcggcaa ttgcgtagta gttgcgtagt agttgcgtag
    11041 gatagttgcg tagggtgggc ttcagcccac caactcccac caactcccac catcttcacc
    11101 ctaaaacccc gtcatctccg caaaagcaat tgcgtagggt gggcttcagc ccaccgtttt
    11161 tcttcatttt cggttgttga ttggcaggct gaagcccacc ctgcggttac aaaaatcccg
    11221 tcattcccgc gcaggcggga atccagacct ttcggggcgg aaacttatcg gataaaacga
    11281 tttcccctgc atcctggatt cccactttca tgggaatgac gggatatagg tttccctacg
    11341 gacgtgttca gattcccgcc ttcgcgggaa tgacggcgga gaagtttctg tctttccgat
    11401 aacttaccgt aacttaaaat cccgtcatta ccgcaaaagc agttgctacg gttacaaaaa
    11461 tcccgtcatc cccacgaaag cgaaaatcct gcctgtcgga tttcggtttt ttcgggcgtt
    11521 tcgggaactt ataaatcgtc attcccgcgc aggcgggaat ccagaacgcg ggacggcggc
    11581 aatattcaaa ggttgtctga aaattcagag gttctagatt cccgcctgcg cgggaatgac
    11641 ggcggagcgg tttctgcttt tccgataaat gaccgcaacc taaaacccat ccttcccacg
    11701 aaaacagaaa aacaaaaacc taaaatcccg tcgttctcgc gaaagcagtt gctacgttta
    11761 ccgtcaaaaa tgccgtctga acgctcaagc cttcagacgg cattttttat ttcacaccct
    11821 tacaggtaga atttttcgat gactttcaaa ttgtcgtcca atttgtacac caacggctgg
    11881 ccagtcggga tttccaagcc catgatgtct ttgtcggaaa tgccctcgat gtgttttgcc
    11941 agcgcgcgca gggagttgcc gtgcgccgcc accaagacgc gtttgccgct caaaatcgcg
    12001 ggggcgattt ggtcttccca aaacggtaat acgcgctcca gcgttacttt caggttttcg
    12061 ccgtcgggta cgacatcggc aggcagatgg gcatagcggc ggtctttgtg tgcggaaaac
    12121 tcatcgtctt tgtccaaaag cggcggcagg gtgtcgtagc tgcgccgcca gatgcggact
    12181 tgctcgtcgc cgtattgttc ggcggtttgt tttttgtcca aaccttgcag ttggccgtag
    12241 tggcgttcgt tcagccgcca tgttttgatt tgcggtacga acagttggtc ggattcttcc
    12301 aaaacgatgt tgcaggtctt aatcgcgcgg gtcaggacgg atgtgaaggc gatgtcgaac
    12361 tcatagccgt tttctttcag ttttttaccg gcggcggcag cctcggcaag cccctgctcg
    12421 ctcagcttca cgtcgcgcca gcctgtaaac aggtttttcg cgttccattc gctttgtccg
    12481 tggcggataa ataccagttc catatcgtct ccaatgtgtg aaagtgggaa agccttattt
    12541 ataacatatt ttcacatttc ccgtatttga ttcagattca gacacgcgcc cactatggtt
    12601 tgccgttttg atttacaata atgccctttg ctttacattc cgcatacaca atgtttcaac
    12661 acacaggacg acacataaag caccgcccta tgtgttgccc tgatttggaa ggggttgccc
    12721 cctcccaaat aaagtctgat tctaccgccc cgaaggacag atgtccaagt ggcgggggtt
    12781 caaccgaaaa ggaaatacga taaatatgca accgcacgct ccacataccg attccaatac
    12841 gctgatgctc ggccgatacg ccgaacgcgc ctatctcgaa tacgccatga gcgtggtcaa
    12901 aggccgcgcg ctgcctgaag tttcagacgg ccaaaagccc gttcagcggc gcattttgtt
    12961 tgcgatgcgc gatatgggtt tgacggcggg ggcgaagccg gtgaaatcgg cacgcgtggt
    13021 cggtgagatt ttgggtaaat accatccgca cggcgacagt tccgcctatg aggcgatggt
    13081 gcggatggcg caggatttta ccttgcgcta tcccttaatc gacggcatcg gcaacttcgg
    13141 ttcgcgcgac ggcgacggtg cggcggcgat gcgttacacc gaagcgcggc tgacaccgat
    13201 tgcggaattg ctgttgtccg aaatcaatca ggggacggtg gatttcgtgc cgaactacga
    13261 cggcgcgttt gacgaaccgc tgcacctgcc cgcccgcctg cctatggtct tgctcaacgg
    13321 tgcgtcaggc attgcggtgg gtatggcgac cgaaatccca tcacacaatt tgaatgaagt
    13381 cacacaggcg gcgattgcac tgttgaagaa gccgacgctg gaaaccgccg acctgatgca
    13441 atacatccct gctcccgatt ttgccggagg cggtcaaatc atcacgccgg cggacgaatt
    13501 gcgccgtatt tacgaaaccg gcaagggcag cgtgcgcgtg cgtgcgcgtt acgaaatcga
    13561 gaagctggcg cgcggacaat ggcgcgtcat cgtaaccgag ctgccgccga acgccaattc
    13621 cgccaaaatc cttgctgaaa tcgaagaaca gaccaacccg aaaccgaaag caggcaaaaa
    13681 gcagctcaac caagaccagc tcaatactaa aaagctgatg ctggatttaa tcgaccgcgt
    13741 gcgcgacgag tcggacggcg aacatcccgt gcgccttgta tttgaaccaa aatccagccg
    13801 catcgatacc gataccttca tcaacacgct gatggcgcaa acttcgctgg aaggcaatgt
    13861 gtccatgaac ttggtgatga tggggctgga caaccgcccc gcgcagaaaa acctgaaaac
    13921 gattttgcag gaatggttgg atttccgcac cgttaccgta acacgccgtc tgaaattccg
    13981 tttgaaccaa gtggaaaaac ggctgcacat cctcgaaggc cgtctgaaag tctttctgca
    14041 catcgacgaa gtgattaaag tcatccgcga atcagatgac ccgaaagccg atttgatggc
    14101 ggcgttcggg ctgaccgaaa tccaagccga agacattttg gaaatccgcc tgcgccagtt
    14161 ggcacgtttg gaaggtttca aactcgaaaa agaattgaac gaattgcgcg aagaacaagg
    14221 ccgtctgaac atccttttgg gcgatgaaaa cgaaaagcgc aagctgatta tcaaagagat
    14281 gcaggcggat atgaaacaat acggcgatgc acgccgcacg ctggtggaag aggccggacg
    14341 cgccgtgctg acacaaacca ccgccgacga acccatcaca ctgattctgt cggaaaaagg
    14401 ctggatacgc agccgtgccg gacacaatct cgatttgagc caaaccgcgt tcaaagaagg
    14461 cgaccgcctc aaacaaaccc ttgaaggcag aacggtttta cccgtcgtca tcctcgattc
    14521 attgggcaga acctatacac tcgatgccgc cgaaattccc ggcggacgcg gcgacggcgt
    14581 gccggtttca tccttgatcg agttgcaaaa cggcgcgaaa cccgttgcga tgttgacagg
    14641 attgccggaa caacattatt tattatcaag cagcggcggc tacggcttca tcgccaagtt
    14701 ggctgatatg gtcggacgcg taaaagcggg caaagtggtg atgaccacag acagtggcga
    14761 aaccgtcctg ccgccgattg ccgtctatgc ctcctcgttc atcaaccccg actgcaaaat
    14821 cattgccgcc accagtcaaa accgcgccct cgccttcccc atcggcgaat tgaaaattat
    14881 ggcgaaaggc aaagggctgc aaatcatcgg attaaacgcc ggcgaatcgc tgacgcatac
    14941 cgccgtttct tccgagccgg aaatcctgat tgaaagcgaa ggcaggcgcg gcgcggcgca
    15001 caaagaccgc atccccgtct ccctgcttga ggcaaaacgc ggcaaaaaag gcaaactatt
    15061 acccatatcg ggcagcctga aacagctttc ttcccccaaa taaacccgat tccacacata
    15121 attatggtga tttccaaccc ccgcgaactt gaaaaactca aagaccggat tcccaatctg
    15181 atcaacatca tccgcgtcgc catcgttttt ccgctgatga ttatgcacat cctcgggctg
    15241 gaaaccggca gccgtgcgaa cctgcacgct tcgtggacgg cgtgggcgtt ttatgtttgg
    15301 ctcgccattg cctgctggct gattttcttt tccatcatcc atccgcattg gcaatggcag
    15361 gcgctgaaaa tgccgcgttt cagcgcggtg gcggacatca cgatgatcgg cgtgctgacc
    15421 tacctgttcg gcggcatcga ttccggcttc ggcatcctga ttctgccctt cgtcgtctgc
    15481 tcctgcctgc tcagctacgg gcgttacccc ctgctctatg ccagctacgc cgccatcctg
    15541 ctgatattca acgccattgc cgacggcgat atcggcaaat atccgcacat atcggatgcc
    15601 cgaaccgcct cggcaacctt catcctcgtc gccgcctcct atctttccgc catcttcacc
    15661 tcactgtcgg tcagatacat cgacagcgcc ggccaactgg cccgcgacaa ccacctcgcc
    15721 taccggcgca tcaaaggctt gagccaaacc gtactcgaac gcgttcaaga agccgtcgtc
    15781 gtcatcaata ccgaagggct ggcggtgctg ttcaaccgga aggcgaaaga ccttttcccc
    15841 gcactcgaaa tcggacggcg cgccgacctg tccgattctg ccgccgaact gtgggaccaa
    15901 gcctctccgc acactttcga atacgtcctc ggcacactcg gcctgaccgc ccgcatccgc
    15961 gccgtgccga tgaacaaaga gccggacaag ctgcttatcc tctacatccg cccgcaaagc
    16021 gaaattcagg cagaagccct gtccgtcaaa cttgccgcgc tcggacaact gaccgccaac
    16081 cttgcccacg aaatccgcaa cccgatgtcc gccatccgcc acgccaacga cctgctgcgc
    16141 gaaaatatgg aagcgggggc ggcagatccg ttcaacgcca aattgtgcaa aatcatcgac
    16201 ggcaacgtct gccgcatcga caaaatgctc gaagacattt cctcactcaa caagcgcaac
    16261 aaaaccgaac gcgaaaccat cggcctgata ccgttttggg aagaattcaa acaagagttc
    16321 ctgctcggcc atcccgatgc cgccggctgc atccgcttgg atatgcaggg caatcacccg
    16381 accgcctatt tcgatcccgc ccacctgcgg cagattatgt ggaatctcgc caacaacgcg
    16441 tggcggcaca gccgcaaaca gcccggctcg atttccgtca ccatccgccc cgcgcaaaaa
    16501 aacaccgtct gcatcctctt tgccgaccgc ccgaagtgca ggaacacctg ttcgaaccct
    16561 tttacaccac ggcggaaaac ggcaccggcc tcgggctgta tgtcgcccgc gaactggcgc
    16621 acgccaattt cggcgatttg acctacctac cggaagccaa atgtttcgaa ctcacattac
    16681 cggaaaaaac caatgactga actgcaacac cccgtcctcg tcgtcgatga cgaaaccgac
    16741 attctcgacc tgatggaaat gaccctgatg aaaatgggct tgcgcgtcca taccgcgtca
    16801 ggcgttgccg aagccaaaaa caagctcgac agccaacgct attcgctcgt cctgaccgat
    16861 atgcgtatgc cggacggctc ggggctggaa gtcgtccaac acatcaacag ccgcctgctc
    16921 gatacgccgg ttgccgtcat caccgccttc ggcaacgccg atcaggcaca ggaagcgttg
    16981 cgttgcggcg cgttcgaccc cgataccatg cagatacagg actatctcga ccaaatcgaa
    17041 cgcgacatta tcgaacaaac cctcaaacaa accgaaggca accgcacgca ggccgccaaa
    17101 cgcttgggca tcagcttccg ttccatgcgc taccgtatgg aacgcctcaa catcggctga
    17161 cgacaaaacg gcatccgcac catctccgcc cacccgaaaa aatgccgtct gaaacggcac
    17221 gggaaagcgg gttcgcccca cgcccgaacg gacacaaaac accatgaccg acatccttat
    17281 tgacaacacc gccaccgaaa ccgtccgcac cctgatacgg gcattccccc ttgtgcccgt
    17341 ttcccaaccg cccgaacaag gcagttacct ccttgccgaa cacgataccg tcagcctcag
    17401 gcttgtcggg gaaaaaagca gcgtcatcgt cgattttgcc tccggcgcgg cacaataccg
    17461 gcgcacaaaa ggcgggggcg aactcatcgc caaagccgtc aaccacaccg cgcaccccac
    17521 cgtttgggac gcaaccgcag gattggggcg cgacagcttc gtcctcgcct cgctcgggct
    17581 ggccgttacc gccttcgagc aacatcccgc cgtcgcctgc ctgctttcag acggcatccg
    17641 ccgcgccctc ctcaatcccg aaacgcaaga caccgccgcg cgcatcaccc tgcatttcgg
    17701 caacgccgca gtacaaatgc ccgcacttgt tcaaacacaa ggcaaacccg acatcgtcta
    17761 tctcgacccc atgtatcccg aacgccgcaa aagtgccgcc gtcaaaaaag aaatgaccta
    17821 cttccaccgg ctcgtcggtg aagcgcaaga tgaggccgcc ctcctccata ccgcacgcca
    17881 aacggcaaaa aaacgcgtcg tcgtcaaacg cccccgcctc ggcgaacacc ttgccggaca
    17941 agcccctgcc taccaataca ccggcaaaag cacccgcttc gacgtttacc tgccctacgg
    18001 gacggacaag ggataacgcc cataaaacaa gacaccgaaa aatttgccgt tcttatgcaa
    18061 acgagaaacc ggtttttgcg tttcgactgt tttggataaa tcatcacacc ttaaagtttg
    18121 tcattcccac ggaagtggga atccagaaat gaaaagcaac aggaatttat cgtaaatgac
    18181 cgaaaccgaa c
//
LOCUS       DAWFAN010000026        18113 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWFAN010000026 DAWFAN010000000
VERSION     DAWFAN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.026"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(84..1187)
                     /locus_tag="WGO65_000605"
     CDS             complement(84..1187)
                     /locus_tag="WGO65_000605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ0326933.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1422..2978)
                     /gene="dnaA"
                     /locus_tag="WGO65_000606"
     CDS             complement(1422..2978)
                     /gene="dnaA"
                     /locus_tag="WGO65_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ0326934.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPNEPRQAAGSASRPELEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VSASSRFMNRPVIDMDLARTALQDIIAEKHKVITADLIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3271..3405
                     /gene="rpmH"
                     /locus_tag="WGO65_000607"
     CDS             3271..3405
                     /gene="rpmH"
                     /locus_tag="WGO65_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ0326935.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3408..3773
                     /locus_tag="WGO65_000608"
     CDS             3408..3773
                     /locus_tag="WGO65_000608"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ0326936.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVIGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRPTAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3838..4065
                     /gene="yidD"
                     /locus_tag="WGO65_000609"
     CDS             3838..4065
                     /gene="yidD"
                     /locus_tag="WGO65_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ0326937.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHGHDPVP"
     gene            4238..5875
                     /gene="yidC"
                     /locus_tag="WGO65_000610"
     CDS             4238..5875
                     /gene="yidC"
                     /locus_tag="WGO65_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ0326938.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDETKPFVLFGDGKEYT
                     YVAQSELLDAQGNNVLKGIGFTAPQKQYSLNGDKVEVRLSAPETNGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSAWILQPKGGQSVCAAGDCRIDI
                     KRRNDNLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(5956..6669)
                     /locus_tag="WGO65_000611"
     CDS             complement(5956..6669)
                     /locus_tag="WGO65_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ0326939.1"
                     /translation="MVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQEIISQTVVQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(6728..7318)
                     /gene="maf"
                     /locus_tag="WGO65_000612"
     CDS             complement(6728..7318)
                     /gene="maf"
                     /locus_tag="WGO65_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ0326940.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAHKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7466..7969
                     /locus_tag="WGO65_000613"
     CDS             7466..7969
                     /locus_tag="WGO65_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ0326941.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSLHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGMLLEKELDLRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSS"
     gene            8003..8182
                     /gene="rpmF"
                     /locus_tag="WGO65_000614"
     CDS             8003..8182
                     /gene="rpmF"
                     /locus_tag="WGO65_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ0326942.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8357..8884
                     /locus_tag="WGO65_000615"
     CDS             8357..8884
                     /locus_tag="WGO65_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ0326943.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            8972..10027
                     /gene="plsX"
                     /locus_tag="WGO65_000616"
     CDS             8972..10027
                     /gene="plsX"
                     /locus_tag="WGO65_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ0326944.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTAQIVGMDEAPQSALKNKKDSSMRVAVNQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            10163..10421
                     /locus_tag="WGO65_000617"
                     /pseudo
     CDS             10163..10421
                     /locus_tag="WGO65_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
     gene            10448..10864
                     /locus_tag="WGO65_000618"
     CDS             10448..10864
                     /locus_tag="WGO65_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326945.1"
                     /translation="MKTSTVVFGGFFITDNGERIQIPVLENPGIKEINHFFSISNFEK
                     KAGVLVFRIIPEPEFGDTELTVYFEKGYYLPMIQTILEDGDIEVKNLKTENYSGKARE
                     ILGDSYPIEHTSKNISTIQNIISEFMIQKQTPAPII"
     gene            10884..11087
                     /locus_tag="WGO65_000619"
     CDS             10884..11087
                     /locus_tag="WGO65_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326946.1"
                     /translation="MPSEAAWAFRRHFLRLKQHIGNPAPPVHGNLHLKTICIPNHTPI
                     PISVICTLPVLGNHRFFAYNSRI"
     gene            11114..12076
                     /locus_tag="WGO65_000620"
     CDS             11114..12076
                     /locus_tag="WGO65_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ0326947.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            12123..12494
                     /locus_tag="WGO65_000621"
     CDS             12123..12494
                     /locus_tag="WGO65_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ0326948.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            12520..13446
                     /gene="fabD"
                     /locus_tag="WGO65_000622"
     CDS             12520..13446
                     /gene="fabD"
                     /locus_tag="WGO65_000622"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ0326949.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFANAVKLVRLRAELMQSAVPQGVGAMAAILGLEDGQVKAICAESAQGEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQIAAFIEAH"
     gene            13585..15450
                     /gene="msbA"
                     /locus_tag="WGO65_000623"
     CDS             13585..15450
                     /gene="msbA"
                     /locus_tag="WGO65_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ0326950.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            15550..17115
                     /gene="guaA"
                     /locus_tag="WGO65_000624"
     CDS             15550..17115
                     /gene="guaA"
                     /locus_tag="WGO65_000624"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ0326951.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRDIFDDAPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAKEKKLTNAKWLAQGTIYPDVIES
                     AGAKTQKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            17177..17923
                     /gene="fabG"
                     /locus_tag="WGO65_000625"
     CDS             17177..17923
                     /gene="fabG"
                     /locus_tag="WGO65_000625"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ0326952.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQAFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         4541 a         5496 c         4339 g         3737 t
ORIGIN      
        1 ttccgacagc cgaacaaaac cccgaatccg tctgcacggt tcggggtata tctccaatac
       61 gggcatcgtg ttccggaaaa ccgtcaaatc cgcatcggca tcacaatata tttgaaattc
      121 ggattgttcg gcacggtaaa cagcgtcgag cggttggcat cgccgaaggc aagctgcata
      181 tcgtcggaat ggatgttgcg caacacgtcc atcagatagc cgatattgaa accgacttcg
      241 agttcgccgc cctgataggc gatttcgatt tcttcgcgcg cttcttcctg ctcgttgttg
      301 ctgcacacaa cgctcaacag gccgggttgc aggaacaggc gcgcgccacg gaatttttcg
      361 ttggcaagaa tggacacgcg ttccagcgca cccaaaagtt cggcacggga caaaacgaaa
      421 atcttgtcgt tgtccaaagg aatcacgcgg ttgaaatcgg ggaatttgcc gtcgatgacc
      481 ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct gtttgtccag cagctcgatt
      541 tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc
      601 aaaatcactt cggcgcgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg
      661 tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg
      721 agataatagc ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg
      781 aaacgctcct gctccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga
      841 aaatcggcgg caggcagggt ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga
      901 cggctgtcgt cccaatccag cgacaccagc gcaccggcag gcagtgcccg taaaatgtcc
      961 tgaaatttct tggcattggt ggtgatgcgg aagtcgtccg cgccgccctc gggacccgca
     1021 gtgtcgattt ggatttccaa atcggttgcc aagagtttgg tctgaccgcc ttttccctca
     1081 atcaggacgt tggacaggat gggcagggtg tggcggcgtt cgacgatgcc ggtaacggct
     1141 tgcagcggct tgagtaggct gtcgcgttcg gcttgtaaaa tcaacatttt cgctcctttg
     1201 aatcggtttg tatagtggat taaatttaaa tcaggacaag gcgacgaagc cgcagacagt
     1261 acaaatagta cgaaaccgat tcacttggta cttcagcacc ttagagaatc gttctctgtg
     1321 agctaaggcg aggcaacgct gtactggttt aaatttaatc cgctatatct ttacccttcg
     1381 gacggcatgg gcaatatcat gtcgtctgaa aacgttttcc atcagttttg aatcagaatc
     1441 agcagtttct cgtaatcctg cgccaactcg gggtcttcct cgcgcagttt cgccaccgcc
     1501 ctgatgccgt gcatgacggt cgtatggtcg cgtccgccga acgaatcgcc gatagacggc
     1561 aggctcaaag tggtcagttc tttggtcagg ctcatggcaa cctgacgcgg acgggcaatg
     1621 ttgcgcgtgc gttttttgcc gagtacgtcg ctgattttga tgcggtaata tttcgccacc
     1681 gcatcaatga tgaggtcggc ggtaatgact ttgtgcttct cggcgataat gtcctgcaac
     1741 gccgtacgcg ccaaatccat atcgatgacg gggcggttca taaagcggct gctcgcgctg
     1801 acgcggttga atgcgccttc aagctcgcgc acattggaac ggatcaggtt ggcaataaac
     1861 aatgccgcct cgtcttcgat gctgataccc gccgcctctg cctttttctg caaaatggcg
     1921 atgcgcattt ccaattcggg cggctcgagt tccaaagtca aaccccaaga gaaacgcgat
     1981 ttgagccggt cgtccatgcc ttcgattttg gcgggtaaaa catcgcaagt gaggatgagc
     2041 tgttttttct cgttgtggaa atggttgtac agatagaaaa actcttccat cgtacggtct
     2101 ttgcctttga tgaactggat atcgtcgata atcagcaggt cgtattgctt gtattgctgc
     2161 ttgaacacgt cgtaggtatt gttgcgaacc gccttcataa agctgcggat gtagtcgtcc
     2221 gaatgcatat agcgcacttt cgcatcggga cggtttttca aaagctcgtt gccgaccgcc
     2281 tgcacaaggt gggttttgcc caaacccgtg ctgccataga ggaagaacgg gttataactc
     2341 tgtcccggac tttccgctat cgcctgcgcg gcggcagctg caaggcggtt gcccttacct
     2401 tcgaccaacg tatcaaacgt gtaatccgga gacaggttgg tttgttcgta acgcgcctct
     2461 tccgcatcac gctgcgcatc cgtccgcgct ttcgcaagca cctccaattc cggacgggaa
     2521 gcagacccgg cagcctgacg cggctcgttc ggcaggtttt tcatacgttc cgccaaaata
     2581 tccgccgccg ttttcgacgc agcgggtttg acaggctctt cagacggcag ctcgtccaac
     2641 agaacctcct gcacaggcat ctcctccgac cccgcgtgca aggacggctc ggcaggttcg
     2701 acagcacctt caaccgccgc catctcataa cgcacgcctt ctcccggttt gaatacgaag
     2761 gcggaacggc cggcagccaa ctcttccctc accgcttcta tttttccggc aaactggctc
     2821 ttgagcatat tgcaggcaaa ctggttcttg ccgtacacca cccatacgcc accctcctca
     2881 ccgacggtaa ggggcgcaat ccattgcgca aactgcccgt gaggcaacat atcgtgaaga
     2941 cggcggaggc acagcggcca aaactctgct aatgtcatgg ataggctcga atcggtaaaa
     3001 atgaaatcga aaacaaagaa aatataatat tttcaaaaag aaaacaaatc tgttgaacac
     3061 acatcggttc aaaatgcagc gaaccgatta taccgactcg ccgatatttt atccacaacc
     3121 cgtgcaaaaa tttatccaca gaaaggcggc agaaatccgc aggcaatcgg gcaatcctcc
     3181 tgcaaagttt ctatattgat tgacaaaagc ggcaaattgg agtgtaattc acggtttaat
     3241 tatctaccca ttctattttt aggaaacatc atgaaacgca cttatcaacc ttccgttacc
     3301 aaacgcaaac gcacccacgg cttcctggtg cgctccaaaa cacgcggcgg ccgcgcagta
     3361 ctggcagcac gccgtgccaa aggccgcaaa cgcctggcag tgtaattttg gactaccgct
     3421 tcggaaggca gtaccgcttg ttaaaaacgg atgatttttc atccgttttt gcattcagaa
     3481 accgccgcag ccgcgacctg ctgcaagttt cgcgctcaaa cggcaacggg ctgggccatc
     3541 cccgcatcgg tctggtgatc ggcaaaaaaa ccgccaaacg cgccaacgaa cgaaattata
     3601 tgaagcgcgt catccgcgac tggttcagat tgaacaaaaa ccggctgccg ccgcaggatt
     3661 tcgtcgtgcg cgtccaccgt aaattcgaca ggcctaccgc aaaacaggca agggcggaac
     3721 tggcacaact catgttcggc aaccccgcaa ccggatgcag gaaacaggca tgatcagaac
     3781 ggtactctgc aggcaaggtt cagacggcaa cgggtttccc atacaaggaa catcccgatg
     3841 aacttcctat tgtccaaact cctgctggga ctgatacggt tctaccaata ttgcatcagc
     3901 ccgctgattc cgccgcgctg ccgttatacg ccgacctgtt cgcaatacgc ggtcgaagcg
     3961 gtcaaaaaat acggcgcatt caaaggcggt cggctcgcca tcaagcgcat tgcacgctgc
     4021 caccctttcg gcggacacgg acacggacac gaccccgttc cctgacccga cgcaatattc
     4081 aaattgcacg ctttcctttt atttcccatc ggtttctata taatgccgtc tgaaccttcg
     4141 ggctggcggc acgaccgtcg ggtatgaagc ccgcccttat tccccgtcta tcggaacacg
     4201 caacctgcgg catttccgac cattcaggaa actctatatg gattttaaaa gactcacggc
     4261 gtttttcgcc atcgcgctgg tgattatgat cggctgggaa aaaatgttcc ccaccccgaa
     4321 acccgtcccc gcaccccaac agacggcaca aaaacaggca gcaaccgctt ccgccgaagc
     4381 cgcgctcgcg cccgcaacgc cgattaccgt aacgaccgac acggttcaag ccgtcattga
     4441 tgaaaaaagc ggcgacctgc gccggctgac cctgctcaaa tacaaagcaa ccggcgacga
     4501 aaccaaaccg ttcgtcctgt ttggcgacgg caaagaatac acctacgtcg cccaatccga
     4561 acttttggac gcgcaaggca acaacgttct gaaaggcatc ggctttaccg caccgcaaaa
     4621 acaatacagc ctcaacggcg acaaagtcga agtccgcctg agcgcgcccg aaaccaacgg
     4681 actgaaaatc gacaaagtct ataccttcac caaaggcagc tatctggtca acgtccgctt
     4741 cgacatcgcc aacggcagcg gtcaaaccgc caacctgagc gcggactacc gcatcgtccg
     4801 cgaccacagc gaacccgagg gtcaaggcta ctttacccac tcttacgtcg gccctgttgt
     4861 ttatacccct gaaggcaact tccaaaaagt cagcttctcc gacttggacg acgatgccaa
     4921 atccggcaaa tccgaggccg aatacatccg caaaaccccg accggctggc tcggcatgat
     4981 tgaacaccac tttatgtccg cttggatttt gcagcctaaa ggcggacaaa gcgtttgcgc
     5041 cgctggcgac tgccgtattg acatcaaacg ccgcaacgac aacctgtaca gcaccagcgt
     5101 cagcgtgcct ttagccgcta tccaaaacgg cgcgaaatcc gaagcctcca tcaacctcta
     5161 cgccggcccg cagaccacat ccgtcatcgc aaacatcgcc gacaacctgc aactggcaaa
     5221 agactacggc aaagtacact ggttcgcttc cccactcttc tggctcctga accaactgca
     5281 caacatcatc ggcaactggg gctgggcaat cgtcgttttg accatcatcg tcaaagccgt
     5341 actgtatcca ttgaccaacg cctcttaccg ctctatggca aaaatgcgtg ccgccgcacc
     5401 caaactgcaa gccatcaaag agaaatacgg cgacgaccgt atggcgcaac aacaggcgat
     5461 gatgcagctt tacacagacg agaaaatcaa cccgctgggc ggctgcctgc ctatgctgtt
     5521 gcaaatcccc gtcttcatcg gattgtattg ggcattgttc gcctccgtag aattgcgcca
     5581 ggcaccttgg ctgggttgga ttaccgacct cagccgcgcc gacccctact acatcctgcc
     5641 catcattatg gcggcaacga tgttcgccca aacttatctg aacccgccgc cgaccgaccc
     5701 gatgcaggcg aaaatgatga aaatcatgcc gttggttttc tccgtcatgt tcttcttctt
     5761 ccctgccggt ctggtattgt actgggtagt caacaacctc ctgaccatcg cccagcaatg
     5821 gcacatcaac cgcagcatcg aaaaacaacg cgcccaaggc gaagtcgttt cctaaatgcc
     5881 gcagcatgaa aaatgccgtc tgaaacctgt tcagacggca tttttattgc tcacccccta
     5941 tcggggcgga aatcttcaac ctgcatacat cacaaaaatc gtcgggcgtt ttttcagatt
     6001 gggcatttct tttctttttc gccactgcac aaccgtctgg ctgatgattt cctgagtcgg
     6061 caacgtcaaa tccgtagccg tgcacaagcg cgtttcagga tgcaggtttt ccaccgcatc
     6121 cgccaacaac acatcgttgc gatacggcgt ttcgataaac agctgcgttt cattctgctg
     6181 gcgcgaacgt tgctccaaag ccttcaaact ctgaatccgc tcgttttttt cagacggcag
     6241 ataaccctta aacgcaaaac tctgcccgtt cgcacccgaa gccatcaaag ccagcagcag
     6301 gctggaaggc ccaaccagcg gacgcacttc aaaaccgtgt ttatgcgcca aagccaccag
     6361 attcgcaccc ggatcggcaa cagccgggca acccgcctcg ctgacaatgc ccatactgcg
     6421 cccttcttgc aaaggtttca gcaattccgg caaagtcttc aaatccgtgt gttcgttcag
     6481 cgtttgcaaa ttcagttcgc ggataggcat tgtcacgccc aaatgtttca aatgcgcgcg
     6541 cgccgttttt tccgcttcca cgacaaaatc cgtcagcccg acaatcgcct gttgttcatg
     6601 cggcaacagg cacggcgtgt caggcgtacc caaaggcgta ggaatcaaat acaaaacagg
     6661 agacaccatt ccctcactca tcggttaaaa atgccgtctg aacccttcag acggcataaa
     6721 cgggcagtta cagaacctcc acgccctcat ttttcaaaaa atcaaccaga cggaaaaccg
     6781 gcaaaccgat taaagcattc ggatcggtac tctcaatcct ttcgatcaac aatgcaccca
     6841 aagcctcgct cttcaacgca cacgagcaat aaaccgcatc aggctcgcgc tccaaatagc
     6901 ggaggatgtg tagctcgtcc aacttcctca tcacgaccac cgtcttatcg atatgccgct
     6961 gcatcctgcc cgtaaccgta tttagcagaa cgaccgcgct gtaaaactca atctccctgc
     7021 cgctcaaatg catcaacatc ttgtgcgcat tagcaaggtt catcggcttg ccccactgcc
     7081 tgccgtcgca ccacgccacc tggtccgcac cgacaatcaa cgcctctggg aaacgcccgg
     7141 ccaacgaccg cgccttaccc tcggcaaggc gcaatgccgt ctgaggggcg gattccccca
     7201 acatcggcgt ttcgtcaaaa tcgggggacg ccgcctgaaa ggcaatgccg agcctttcca
     7261 tctgttcgcg gcggaaaacc gaactcgtac ccaaaatcaa aggcagttcc aaacccatcc
     7321 catcctcctt accgttgaaa acacgcccga aggggcggta aaatccagcc atgcgccgaa
     7381 acacggatac acgccttcgg cgtaccgcaa catttttctt aaaaatattg acgttagaac
     7441 atctaaatta tatcatatct cgtttatgtc agaccctaat ttgattgacc cggaaatttt
     7501 cgccgccgaa gggcagaacc tgcaaggcag ttttctgctg gaagaattgg acgagcgagt
     7561 cagtttgcac gattatcccg ccgacaggca gaccaaaata tcgtttacac tgaccggcgg
     7621 tcgcgaccgg ctgcaacgcc tgttcctcga cctgaacgtc aaagccgata tgcccctgat
     7681 ttgccagaga tgtatcaaac ccatgccgtt catgcttgat gaaagcagcc gtatcgtcct
     7741 gttttccaac gaagagtcct tggacgaatc catgcttgcc gatgaagaat tggaaggtat
     7801 gctgctcgaa aaagagcttg acttgcggac cttggtcgaa gaccaaatcc tgatgtcgct
     7861 ccctttctcc ccccggcacg aagactgcgg cgacaatggg acactggaag aagtcaatcg
     7921 ggacaaacca aacccctttg ctgttttggc aggtttgaaa agcagttaat taggacacag
     7981 tttatttatc taggagcttg aaatggccgt tcaacaaaac aaaaaatccc cttccaaacg
     8041 cggtatgcac cgttcgcacg acgcgctgac cgcgcctgca ctgtctgtcg acagcacaac
     8101 cggcgaagta caccgcccgc accacatctc ccccaacggt atgtaccgcg gccgcaaagt
     8161 ggtcaaagcc aaaggcgaat aatccctatt cgactgactg aaaaagccag aacattgcca
     8221 tgcaattact ggcttttttt gcattggacg caccatccgt ccaaactttc gccatacgtc
     8281 aacacacagg ggcaaagcgt tccgtataat accccgtgaa aatattccaa aagccccaac
     8341 caccaaggaa attccgatga aacagaaaat ctggtacacc tacgatgaca tccaccgcgt
     8401 catcaaagca ttggcagaaa aaatccggaa cgccgacatc aaatacgatg ccatgattgc
     8461 catcggcggc ggcggcttta ttccggcacg tatgctgcgc tgttttctgg aaattccgat
     8521 ttatgccgta accaccgcct attacgacag cgacaacgaa ggacaggtta cagaagaagt
     8581 caaaaaagtc caatggctcg accccgttcc cgaagccctg cggggcaaaa acgtccttgt
     8641 tgtcgatgaa gtggacgaca gccgcgtaac catggaattt tgcctgacag agctgcttaa
     8701 agaagacttc ggcaccatcg gcgtcgccgt actgcacgaa aaaatcaaag ccaaagcagg
     8761 caaaatcccc gaaggcattc cctatttcag cggcatcacc gtagaagact ggtggatcaa
     8821 ctatccatgg gacgcactcg acatcgacga acacaaccgc cttgccgagg ccggccgagg
     8881 ctgacccttt cagacggcat attttccgaa ccgatgccgt ctgaagcccg cacgacccct
     8941 gccgcagacc gaaaacctac cggagaaccc tatgattaca cttgccgtag atgccatggg
     9001 cggcgaccaa ggacttgccg ttaccgtacc cggcgcaacc gcattcctcc aagcacaccc
     9061 cgatgtccgc ctgattatga ccggcgacga aacgcaactg cgccaagccc tgaccgcggc
     9121 aggcgcaccg atggaacgca tcgacatctg ccacaccgca caaatcgtcg gcatggacga
     9181 agcgccgcaa tccgccctga aaaacaaaaa agactcctcc atgcgcgttg ccgtcaacca
     9241 agttaaagaa ggcaaagccc aagccgccgt atccgcaggc aacacgggtg cgctcatggc
     9301 aaccgcacgt ttcgtcctca aaaccattcc cggcatcgaa cgccccgcca tcgccaaatt
     9361 ccttccttcc gacaccgacc atgttaccct tgcactcgac cttggcgcga acgtcgactg
     9421 cacgcccgaa cagctcgccc aatttgccgt tatcggcagc gaactcgtcc acgcactcca
     9481 tcctcaaaaa ggacagccgc gcgtcgggct ggtcaacgtc ggcacggaag acatcaaagg
     9541 tacggacacc gtcaaacaaa cctacaaact gctgcaaaac agcaaactca actttatcgg
     9601 caacatcgaa agcaacggca tcctctacgg cgaagcagat gtcgtcgtcg ccgacggctt
     9661 tgtcggcaac gtcatgctca aaaccatcga aggcgcggtc aaattcatga gcggagccat
     9721 ccgccgcgaa ttccaaagca acctgttcaa caaacttgcc gccgttgccg ccctacccgc
     9781 cctcaaaggg ctgaaaaaca aactcgatcc gcgcaaattc aacggggcca tcctgctcgg
     9841 gctgcgcggc atcgtgatta aaagccacgg cggcacagac gaaaccggtt tccgctacgc
     9901 actcgaagaa gcctaccacg aagccaagtc agccggcctt tccaaaatcg aacagggcgt
     9961 agccgaacaa ctcgccgcac tcgaaactgc caaagccgtc caaaacgaaa atgtcggcgg
    10021 tctgtaacac gcgcgatgcc gtccgaacgc ccccgcccct ttcagacggc atccgcccgc
    10081 cccgaacctg cgggcgcgga cggcgatgca cctgtccggc acttcccaaa tacttccctg
    10141 taaaataagg agtatttgaa aaatgaagac attagaaaaa cggatgaggg cattagacaa
    10201 acggataatg aagttcggaa aatcccttga aggcaggctt gaggcttgat gcccgtctga
    10261 ttgaatccgc attggattat attcattatt cggaacgttt tttggctttt gaaatcctgt
    10321 gtacttatat cgaagatttc gatgtccggc tgacggaaca agaatcccgg gaaatttctt
    10381 ttatcaacaa ggaatttgag atagaaagca cgtccgatta accaataaag ccaatgggtt
    10441 gataaacatg aaaacatcaa cggttgtttt tggcggattt tttattacag ataatggaga
    10501 acgaatccaa atccccgttt tggaaaatcc tggcattaaa gaaatcaatc actttttttc
    10561 catatcaaat tttgagaaaa aagccggcgt ccttgttttc agaatcatcc ccgagccgga
    10621 atttggcgat accgaattaa ctgtctattt tgaaaaagga tattatttgc caatgattca
    10681 aacaatttta gaagacgggg atattgaagt caaaaacctt aaaacggaaa attacagtgg
    10741 gaaggcaagg gaaattttgg gagattccta ccctattgag catacctcta aaaatatttc
    10801 tacaattcaa aatataattt ctgaatttat gatacaaaaa caaacaccgg caccaataat
    10861 ttgatggaaa cccaacaatc aaaatgccgt ctgaagcagc ttgggctttc agacggcatt
    10921 tcctccgctt aaaacagcat atcggcaacc ccgccccgcc cgtccacggc aatctgcatc
    10981 tgaaaaccat ctgtatccca aaccacaccc ccatcccgat ttccgtcatt tgcaccctgc
    11041 ccgtattggg caatcatcgg tttttcgctt acaatagccg aatctgaacc aactatccaa
    11101 aaaggccgtt cccatgcagt atgccaaaat ttccggcacg ggcagctatc ttcccgccaa
    11161 ccgcgtcagc aatgacgacc ttgcccaaaa agtggatacc tcggacgagt ggattaccgc
    11221 gcgcacgggc atcaaattcc gccatattgc agccgaaaat gagaaaacca gcgatcttgc
    11281 cgccgaagca gcccgccgcg cactggatgc agccggatta gacagcggcg aaatcgattt
    11341 aattattgtg gcgacggcaa cgccggatat gcagtttcct tctaccgcca ccatcgtcca
    11401 acaaaaactg ggcatcacca acggctgccc cgcgtttgac gtacaggcgg tgtgtgcggg
    11461 ctttatgtac gcgctgacca cggcaaacgc ctacatcaaa agcggcatgg caaaaaacgc
    11521 gctggtcatc ggcgcggaaa ctttcagccg catcgtcgat tggaacgacc gcacaacctg
    11581 cgtattgttc ggcgacggcg cgggcgcggt ggttttgagc gcggcggaca aaccgggcat
    11641 catccacagc aaactcaaag ccgacggcaa ttatctgaaa ctcttaaacg tccccgggca
    11701 aatcgcctgc ggcaaagttt ccggttcgcc gtacatttcg atggacggtc ccggcgtgtt
    11761 caagtttgcc gtcaaaatgc tgtccaaaat cgccgatgac gttatcgaag aagccggtta
    11821 taccgccgct caaatcgact ggatcgtgcc gcatcaggca aaccgccgca ttatcgaatc
    11881 gaccgcgaaa catttaggtt tgagtatgga caaagtcgtc ctgaccgtcc aagaccacgg
    11941 caacacatcc gccgcatcga ttccgctggc tttggatacg ggcatccgca gcggacaaat
    12001 caaacgcggt caaaacctgc tgctcgaagg catcggcggc ggtttcgcgt ggggcgcggt
    12061 gctgttgcaa tattgaaccc gatgccgtct gaaacaggct ttcagacggc atttcccata
    12121 tcatgaagcg gcaggctttc ttcaaaccaa ttgcgtgtgc ggcatttctg tctgccgttt
    12181 cgctgcgcct ccccgtattg ggcgcgtgtt acgcaatatt gtccctctat gcgtttgcac
    12241 tttacggcat cgacaaacgg cgtgccgtgc ggggaaaacg ccgcattccc gaacaccgcc
    12301 tgctgctgcc tgccttgttc ggcggttggg cgggcgcata cttgggcagc aggatattca
    12361 ggcataaaac ggcgaaaaag cgttttgttg tgctgttccg tctgactgtt tcgggcaatg
    12421 tcctggcgac ctgcatcctg attgattatt tcgttccgcc cgaacttttt gtcaaactcg
    12481 ggcaacatct ctgacaatcc aaccaaacaa aggaatacca tgtcttttgc attcttcttt
    12541 cccggacaag gttctcaaag cctcggcatg atgaacggct ttacagggca ggccgtcgtc
    12601 aaaaacacat ttgacgaagc ctccgccata ttggggcagg acttgtgggc gatgattaac
    12661 ggcagcgatg ccgaaatcat cggtcaaacc gtcaataccc aacccatcat gctcgccgcc
    12721 ggcgttgccg tttaccgcgc ctatttggaa gcgggtggca aaacgcctgc cgccgttgcc
    12781 ggacacagcc tcggcgaata taccgcgctt gtcgccgccg gcgcattgga ttttgccaac
    12841 gcggtcaaac tcgtgcgcct acgcgcagaa ctgatgcagt ccgccgtacc gcaaggcgtg
    12901 ggcgcaatgg cggcgattct cggcttggaa gatgggcagg ttaaagccat ttgtgccgaa
    12961 tccgcacagg gcgaagtggt cgaagccgtc aacttcaact cgcccgggca aatcgtgatt
    13021 gcaggcaacg ccgccgccgt cggacgcgcc atggctgccg ccaaagaagc cggagccaaa
    13081 cgcgccctgc cgctgcccgt gtccgtacct tcccattgca gcctcatgaa acccgccgcc
    13141 gacaaacttg ccgaagcctt gaaaaccgtt gaaatcaagc agccgcaaat ccgcgttatc
    13201 cacaacgccg acgttgccgc ctacgatgat gccgacaaaa tcaaagacgc gctcgtccgc
    13261 cagctttaca gccccgtacg ctggacggaa accgtcaacg ccctcgtttc agacggcatt
    13321 gccgaatccg ccgaatgcgg cccgggcaaa gttctggcag gtctggcaaa acgcatcaac
    13381 aaagccgccg cgtgcagcgc actgaccgat gccggacaga ttgccgcctt tatagaagca
    13441 cactgacttc gttctgtaaa aagcagcctg catgcctcag gctgcttttc acgtccgaac
    13501 ggcaaacagc cgcatatttg cgctataatt gatgacgacc aaaccaccga cagcggctgc
    13561 cgttgcagtt cccgccccac cgacatgata gaaaaactga ctttcggact gtttaaaaaa
    13621 gaagacgcgc gcagctttat gcgcctgatg gcgtacgtcc gcccctacaa aatccgcatc
    13681 gttgccgccc tgattgccat tttcggcgtt gccgccaccg aaagctacct tgccgccttc
    13741 atcgcccccc tgattaacca cggcttttcc gcacctgccg cgccgcccga gctgtctgcc
    13801 gccgccggca tcatttccac cctgcaaaac tggcgcgaac agtttaccta tatggtttgg
    13861 gggacggaaa acaaaatctg gaccgtcccg ctctttctca tcattctcgt cgtcatccgt
    13921 ggcatctgcc gctttaccag cacctatctg atgacttggg tctccgtgat gaccatcagc
    13981 aaaatccgca aagatatgtt tgccaaaatg ctgacccttt cctcccgcta ccatcaggaa
    14041 acgccgtccg gcaccgtact gatgaatatg ctcaacctga ccgaacagtc ggtcagtaac
    14101 gccagcgaca tcttcaccgt cctcacgcgc gacacgatga tcgttaccgg cctgaccatc
    14161 gtcctgcttt acctcaactg gcagctcagc ctcatcgttg tcctgatgtt ccccctgctc
    14221 tccctgctct cgcgctacta ccgcgaccgt ctgaagcacg tcatttccga ctcgcaaaaa
    14281 agcataggca cgatgaacaa cgtgattgcc gaaacccatc agggacaccg cgtcgtcaag
    14341 ctgttcaacg ggcaggcgca ggcggcaaac cggtttgacg cggtcaaccg caccatcgtc
    14401 cgcctcagca aaaaaatcac gcaggcaacg gcggcacatt ccccgttcag cgaactgatt
    14461 gcctcgattg ccctcgccgt tgtcatcttc atcgccctgt ggcaaagcca aaacggctac
    14521 accaccatcg gcgaatttat ggcattcatc gtcgcgatgc tgcaaatgta cgcacccatc
    14581 aaaagccttg ccaacatcag catccctatg cagacgatgt tcctcgccgc cgacggcgta
    14641 tgtgcatttc tcgacacccc gcccgaacag gacaaaggca cgctcgcgcc gcagcgtgtc
    14701 gaagggcgta tcagcttccg caacgtcgat gtcgaatacc gttcggacgg catcaaagcc
    14761 ctcgacaact tcaacctcga catcagacaa ggcgaacgcg tcgccctggt cggacgttcc
    14821 ggcagcggca aatccaccgt cgtcaacctg ctgccccgct ttgtcgaacc gtctgccggc
    14881 aacatctgca tagacggtat cgacatcgcc gacatcaaac tcgactgcct gcgcgcccaa
    14941 ttcgccctcg tctcccaaga cgtattcctg tttgacgaca ccctgtttga aaacgtccga
    15001 tacagccgtc ccgacgcagg cgaagccgaa gtcctgtccg ccctccaagc cgccaacctg
    15061 caaagcctga ttgacagctc cccgctcgga ctgcaccagc ccatcggatc gaacggcagc
    15121 aacttgtccg gcggacagcg gcaacgcgtc gccatcgccc gcgccatttt gaaagacgcg
    15181 ccgatattat tattggacga agccaccagc gcattagaca acgaatccga acgcctcgtc
    15241 caacaagcgc tcgaacgcct gatggaaaac cgcaccggca tcatcgtcgc ccaccgcctg
    15301 accaccatcg aaggggccga ccgcatcatc gtaatggacg acggcaaaat catcgaacaa
    15361 ggcacacacg aacaactgat gtcccaaaac ggctactaca cgatgttacg caatatctca
    15421 aacaaagatg ccgccgtccg gacggcataa acaaaatgcc gtccgaaatg gtataatcgc
    15481 cccgaccctt tcagacggca ctatatccgc cgacccatcc gattatcttc aatcactgta
    15541 aaacccatta tgacccaaga caaaatcctc atcctcgact tcggttcgca agttacccag
    15601 ctgattgccc gccgcgtgcg cgaagcccac gtttactgcg aactgcattc cttcgatatg
    15661 cctttggacg aaatcaaagc cttcaacccc aaaggcatca tcctttccgg cggccctaat
    15721 tccgtttacg aatccgacta tcaggccgat accggtattt ttgatttggg cattccggtt
    15781 ttgggcatct gctacggaat gcagtttatg gcgcaccact tgggcggcga agtgcagccc
    15841 ggcaaccagc gcgaattcgg ttacgcgcaa gtcaaaacca tcgacagcga actgacccgc
    15901 gatattttcg acgacgcgcc caacacactc gacgtatgga tgagccacgg cgacaaagtg
    15961 tccaaactgc ccgacggctt cgccgtcatc ggcaacaccc cgtcctgccc gattgccatg
    16021 atggaaaacg ccgaaaaaca attctacggc atccagttcc accccgaagt tacccacacc
    16081 aaacaaggcc gcgccctgtt gaaccgcttt gtcttggata tttgcggcgc gcaaccgggc
    16141 tggacgatgc cgaactacat cgaagaagcc gttgccaaaa tccgcgaaca ggtcggcagc
    16201 gacgaagtga ttttaggttt gtcaggcggc gtagactctt ccgtagccgc cgcgctgatt
    16261 caccgcgcca tcggcgacca actgacctgc gtgttcgtcg atcacggttt gttgcgcctg
    16321 aacgaaggca aaatggtgat ggatatgttc gcccgcaact tgggtgtgaa agtgattcac
    16381 gtcgatgccg aagggcagtt tatggcgaaa ctcgccggcg ttaccgaccc tgagaaaaaa
    16441 cgcaaaatca tcggcgcgga atttatcgaa gtatttgatg ccaaagagaa aaaactcacc
    16501 aacgccaaat ggctggcgca aggcacgatt taccccgacg taatcgaatc cgcaggcgcg
    16561 aaaacccaaa aagcccacgc catcaaatcg caccacaacg tcggcggtct gcccgaaaat
    16621 atgaagctca agctgcttga gccgttgcgc gacttgttta aagacgaagt gcgcgaactg
    16681 ggcgttgctt tgggtctgcc gcgcgaaatg gtgtaccgcc atccgttccc cggccccggt
    16741 ttgggcgtgc gcatcttggg cgaagtgaaa aaagaatacg ccgacttatt gcgtcaagca
    16801 gacgatattt tcatccaaga attacgcaac accaccgacg aaaacggcac atcttggtac
    16861 gacctgacca gccaggcatt tgccgtattc ctgcctgtga aatccgtcgg cgtaatgggc
    16921 gacggacgca cttacgacta cgtcgtcgca ctgcgcgcag tcatcaccag cgacttcatg
    16981 accgcacact gggcagaact gccttactca ctgctcggac gcgtgtccaa ccgcatcatc
    17041 aacgaagtca aaggcatcaa ccgcgtggtg tacgatgtca gcggcaaacc gcctgccacc
    17101 atcgagtggg aataaccagc aaacagggct gcaccgtccg gcgcagtctt tcgattatcg
    17161 gaaaaaagga aaaaatatga gcacacaaga tttaagcggc aaaatcgctt tggtaaccgg
    17221 cgcatcgcgc ggtatcggtg cagcaattgc cgacacactg gcggcagccg gtgccaaagt
    17281 catcggtacg gcgaccagtg agagcggtgc ggcggcgatt agcgagcggt tggcgcaatg
    17341 gggcggcgaa ggccgcgtat taaattccgc cgaacccgaa accgtcgaaa acctgattgc
    17401 cgacatcgaa aaaacgttcg gcaaactcga tattctggtc aacaacgccg gcatcacccg
    17461 cgacaacctc ctgatgcgca tgaaagaaga agagtgggac gacatcatgc aggtcaacct
    17521 caaatccgtg ttccgcgctt ctaaagccgt cttgcgcggc atgatgaagc agcgcgccgg
    17581 ccgtatcatc aacatcacat ccgtcgtcgg cgtgatgggc aatgccggtc aaaccaacta
    17641 tgccgcggca aaagcaggct taatcggttt ctccaaatcc atggcgcgcg aagtcggcag
    17701 ccggggcatt accgtcaact gcgtcgcccc cggctttatc gataccgata tgacccgcgc
    17761 cctgccggaa gaaacccgcc aagcctttac cgcccaaacc gccttgggca gattcggcga
    17821 cgcgcaagac attgccgatg cggttttgtt cctcgcttcc gatcaggcga aatacattac
    17881 cggccagacg ctgcacgtca acggcggcat gctgatgccc tgacggacag ccgtgccgac
    17941 aatgccgtct gaagcccttt cagacggcat ttgcattccc aaacaaaatg aacacacacc
    18001 acaccctgcc ctgcccctgc ggttcaggca caagctgaga cctttgcaaa actcctttcc
    18061 ctcccgacag ccgaaaccca aacacaggtt ttcgtctatt ttcgccccaa ata
//
LOCUS       DAWFAN010000027        17817 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWFAN010000027 DAWFAN010000000
VERSION     DAWFAN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17817
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.027"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..303
                     /locus_tag="WGO65_000626"
     CDS             <1..303
                     /locus_tag="WGO65_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0326953.1"
                     /translation="KPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDV
                     TGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
     gene            321..632
                     /gene="rpsJ"
                     /locus_tag="WGO65_000627"
     CDS             321..632
                     /gene="rpsJ"
                     /locus_tag="WGO65_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ0326954.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            753..1077
                     /locus_tag="WGO65_000628"
                     /pseudo
     CDS             753..1077
                     /locus_tag="WGO65_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1321..1965
                     /gene="rplC"
                     /locus_tag="WGO65_000629"
     CDS             1321..1965
                     /gene="rplC"
                     /locus_tag="WGO65_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ0326955.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1965..2585
                     /gene="rplD"
                     /locus_tag="WGO65_000630"
     CDS             1965..2585
                     /gene="rplD"
                     /locus_tag="WGO65_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ0326956.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2582..2896
                     /gene="rplW"
                     /locus_tag="WGO65_000631"
     CDS             2582..2896
                     /gene="rplW"
                     /locus_tag="WGO65_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ0326957.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            2902..3735
                     /gene="rplB"
                     /locus_tag="WGO65_000632"
     CDS             2902..3735
                     /gene="rplB"
                     /locus_tag="WGO65_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ0326958.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3742..4020
                     /gene="rpsS"
                     /locus_tag="WGO65_000633"
     CDS             3742..4020
                     /gene="rpsS"
                     /locus_tag="WGO65_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ0326959.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            4029..4358
                     /gene="rplV"
                     /locus_tag="WGO65_000634"
     CDS             4029..4358
                     /gene="rplV"
                     /locus_tag="WGO65_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ0326960.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4368..5060
                     /gene="rpsC"
                     /locus_tag="WGO65_000635"
     CDS             4368..5060
                     /gene="rpsC"
                     /locus_tag="WGO65_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ0326961.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            5044..5460
                     /gene="rplP"
                     /locus_tag="WGO65_000636"
     CDS             5044..5460
                     /gene="rplP"
                     /locus_tag="WGO65_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ0326962.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5460..5651
                     /gene="rpmC"
                     /locus_tag="WGO65_000637"
     CDS             5460..5651
                     /gene="rpmC"
                     /locus_tag="WGO65_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ0326963.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5651..5914
                     /gene="rpsQ"
                     /locus_tag="WGO65_000638"
     CDS             5651..5914
                     /gene="rpsQ"
                     /locus_tag="WGO65_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ0326964.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            6136..6504
                     /gene="rplN"
                     /locus_tag="WGO65_000639"
     CDS             6136..6504
                     /gene="rplN"
                     /locus_tag="WGO65_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ0326965.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6516..6839
                     /gene="rplX"
                     /locus_tag="WGO65_000640"
     CDS             6516..6839
                     /gene="rplX"
                     /locus_tag="WGO65_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ0326966.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6849..7388
                     /gene="rplE"
                     /locus_tag="WGO65_000641"
     CDS             6849..7388
                     /gene="rplE"
                     /locus_tag="WGO65_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ0326967.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7391..7696
                     /gene="rpsN"
                     /locus_tag="WGO65_000642"
     CDS             7391..7696
                     /gene="rpsN"
                     /locus_tag="WGO65_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ0326968.1"
                     /translation="MAKKALINRNLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7712..8104
                     /gene="rpsH"
                     /locus_tag="WGO65_000643"
     CDS             7712..8104
                     /gene="rpsH"
                     /locus_tag="WGO65_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ0326969.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            8118..8651
                     /gene="rplF"
                     /locus_tag="WGO65_000644"
     CDS             8118..8651
                     /gene="rplF"
                     /locus_tag="WGO65_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ0326970.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8665..9018
                     /gene="rplR"
                     /locus_tag="WGO65_000645"
     CDS             8665..9018
                     /gene="rplR"
                     /locus_tag="WGO65_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ0326971.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            9037..9555
                     /gene="rpsE"
                     /locus_tag="WGO65_000646"
     CDS             9037..9555
                     /gene="rpsE"
                     /locus_tag="WGO65_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ0326972.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9548..9733
                     /gene="rpmD"
                     /locus_tag="WGO65_000647"
     CDS             9548..9733
                     /gene="rpmD"
                     /locus_tag="WGO65_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ0326973.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            9735..10169
                     /gene="rplO"
                     /locus_tag="WGO65_000648"
     CDS             9735..10169
                     /gene="rplO"
                     /locus_tag="WGO65_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ0326974.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            10181..11491
                     /gene="secY"
                     /locus_tag="WGO65_000649"
     CDS             10181..11491
                     /gene="secY"
                     /locus_tag="WGO65_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ0326975.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11496..11714
                     /gene="infA"
                     /locus_tag="WGO65_000650"
     CDS             11496..11714
                     /gene="infA"
                     /locus_tag="WGO65_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ0326976.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11735..11848
                     /gene="rpmJ"
                     /locus_tag="WGO65_000651"
     CDS             11735..11848
                     /gene="rpmJ"
                     /locus_tag="WGO65_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ0326977.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            11914..12276
                     /gene="rpsM"
                     /locus_tag="WGO65_000652"
     CDS             11914..12276
                     /gene="rpsM"
                     /locus_tag="WGO65_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ0326978.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12296..12691
                     /gene="rpsK"
                     /locus_tag="WGO65_000653"
     CDS             12296..12691
                     /gene="rpsK"
                     /locus_tag="WGO65_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ0326979.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12711..13331
                     /gene="rpsD"
                     /locus_tag="WGO65_000654"
     CDS             12711..13331
                     /gene="rpsD"
                     /locus_tag="WGO65_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ0326980.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13357..14343
                     /gene="rpoA"
                     /locus_tag="WGO65_000655"
     CDS             13357..14343
                     /gene="rpoA"
                     /locus_tag="WGO65_000655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ0326981.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14367..14735
                     /gene="rplQ"
                     /locus_tag="WGO65_000656"
     CDS             14367..14735
                     /gene="rplQ"
                     /locus_tag="WGO65_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ0326982.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14882..15595
                     /gene="minC"
                     /locus_tag="WGO65_000657"
     CDS             14882..15595
                     /gene="minC"
                     /locus_tag="WGO65_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ0326983.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15624..16439
                     /gene="minD"
                     /locus_tag="WGO65_000658"
     CDS             15624..16439
                     /gene="minD"
                     /locus_tag="WGO65_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ0326984.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16443..16706
                     /gene="minE"
                     /locus_tag="WGO65_000659"
     CDS             16443..16706
                     /gene="minE"
                     /locus_tag="WGO65_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ0326985.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16710..17630
                     /gene="oxyR"
                     /locus_tag="WGO65_000660"
     CDS             16710..17630
                     /gene="oxyR"
                     /locus_tag="WGO65_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ0326986.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(17627..>17817)
                     /locus_tag="WGO65_000661"
     CDS             complement(17627..>17817)
                     /locus_tag="WGO65_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0326987.1"
                     /translation="LIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNIL
                     AYRVVGSGKWRFENNACA"
BASE COUNT         4872 a         3439 c         4407 g         5099 t
ORIGIN      
        1 aaaccgggta ctatcactcc tcacaccaag ttcaaagcag aagtgtacgt attgagcaaa
       61 gaagagggtg gccgccatac cccgttcttc gccaactacc gtccgcaatt ctacttccgt
      121 accaccgacg taaccggcgc ggttactttg gaagaaggtg tggaaatggt aatgccgggt
      181 gagaacgtaa ccattaccgt agaactgatt gcgcctatcg ctatggaaga aggcctgcgc
      241 tttgcgattc gcgaaggcgg tcgtaccgtg ggtgccggcg tggtttcttc tgttatcgct
      301 taattgaagg atattgataa atggcaaacc aaaaaatccg tatccgcctg aaagcttatg
      361 attacgccct gattgaccgt tctgcacaag aaatcgttga aactgcaaaa cgtaccggtg
      421 ccgttgtaaa aggtccgatt ccgttgccga ccaaaatcga acgtttcaac attctgcgtt
      481 ctccgcacgt gaacaaaact tcccgtgaac aattggaaat ccgcacccac ttgcgcctga
      541 tggacatcgt ggattggacc gataaaacta ccgatgcgct gatgaagctg gatttgccgg
      601 ccggtgttga tgtagaaatc aaagtccaat aattcggact attgaaaatc cccaagcaat
      661 caatgcttgg ggatttttta tgttatgccg agacctttgc aaaattcccc aaaatcccct
      721 aaattcccac caagacattt aggggatttc tcatgagcac cttcttccaa caaaccgccc
      781 aagccatgat tgccaaacac atcgaccgtt tcccgctatt gaagttggac caggtgattg
      841 attggcagcc gatcgaacaa tacctgaacc gtcaaaaatc cgttacctcc gagattaccg
      901 cggccgtccc gcctacccgc tgttgtccat gttcaaagcc gttctgctcg gacaatggca
      961 cagcctctcc gatcccgaac tcgagcacag cctcatcacc cgcatcgatt tcaacctgtt
     1021 ttgccgcttt gacgaactga gcatccccga ttacagtcat caaccatatt ccggtttgtc
     1081 ggagaaagat gcatacgctg tgatgaccgg ataccgaccc tttaaaagag tctgacccta
     1141 tgccgtctga aaattcaaaa cgcttcagac ggcatattga agatatttct gatatttctg
     1201 ttgatatttc tttaacttgt cagatataat gccgagcttg gtacatttgt gccaagttta
     1261 actttgtctg aaagacaggc caatcgtagc ctgtcccttt actttaaaag gaaaataatc
     1321 atgactttag gtctggttgg acgcaaagtt ggtatgaccc gcgtgttcga cgaacagggt
     1381 gtttctgttc cggtaaccgt tttggatatg tctgccaacc gcgttacaca agtaaaatcc
     1441 aaagatactg acggctatac tgccgttcaa gttacctttg gtcagaaaaa agccaatcgt
     1501 gtcaacaaag ccgaagccgg gcactttgca aaagcaggtg ttgaagccgg tcgcggtttg
     1561 attgagtttg ctttgactga agaaaaactg gctgaattga aagctggtga cgaaatcacc
     1621 gtttctatgt ttgaagtcgg tcaactggtc gatgtaaccg gtacctctaa aggtaaaggt
     1681 ttctccggca cgattaaacg tcataacttc ggcgcccaac gtacttccca cggtaactcc
     1741 cgctctcacc gcgttccagg ttctatcggt atggcacaag acccgggtcg cgtgttcccc
     1801 ggtaagcgca tggccggcca atacggcaac accaaggcaa ctgttcaaaa attggaagtt
     1861 gtccgtgttg atgcagaacg ccaactgctg ttggttaagg gtgctgttcc gggtgcggtc
     1921 aacagcgatg ttgtagttcg tcccagcgtg aaagtaggtg cgtaatggaa ttgaaagtaa
     1981 ttgacgctaa aggacaagtt tcaggcagcc tgtctgtttc tgatgctttg ttcgcccgcg
     2041 aatacaatga agcgttggtt catcagctgg taaatgccta cttggcaaac gcccgctccg
     2101 gtaaccgcgc tcaaaaaacc cgtgccgaag taaaacactc aaccaaaaaa ccatggcgtc
     2161 aaaaaggtac cggccgtgcc cgttccggta tgacttcttc tccgctgtgg cgtaaaggtg
     2221 gtcgcgcgtt cccgaacaaa cccgacgaaa acttcactca aaaagtaaac cgcaaaatgt
     2281 accgtgccgg tatggcgact attctgtccc aattgactcg tgacgagcgt ttgtttgcga
     2341 ttgaggcgtt gactgccgaa actcctaaaa ccaaagtttt tgctgaacaa gtgaaaaatc
     2401 tgggtctaga gcaagtgttg tttgtaacca aacagctcga cgagaatgtt tacttggctt
     2461 cacgcaactt gccaaacgtg ttggttttgg aagctcaaca agttgatcct tacagcttgc
     2521 tgcgttacaa aaaagtaatc atcactaaag atgcagttgc acaattagag gagcaatggg
     2581 tatgaatcaa caacgtttga ctcaagtgat tttggcacct atcgtttctg aaaaaagcaa
     2641 cgtattggct gaaaaacgca accaaatgac gtttaaagtt ttggcaaatg caaccaaacc
     2701 tgaaatcaaa gcggctgttg agctgctgtt tggtgttcaa gttgcttctg taactaccgt
     2761 tacaactaaa ggcaaaacta agcgttttgg tcgtactttg ggccgccgca gcgatgttaa
     2821 aaaagcttat gtaagcttgg ctgccggtca agagttggat ttggaagccg ctgctgcagc
     2881 tgcagataag gaataaacaa aatggcaatc gttaaaatga agccgacctc tgcaggccgt
     2941 cgcggcatgg ttcgcgtggt aacagaaggt ttgcacaaag gtgcgcctta tgcacctttg
     3001 ctcgaaaaga aaaattctac tgccggtcgt aacagtaatg gtcatatcac cacccgtcac
     3061 aaaggcggcg gtcataaaca ccattaccgt gttgtagact ttaaacgtaa caaagacggc
     3121 attcctgcta aggtagagcg tatcgaatac gatcctaacc gtactgcctt catcgcactg
     3181 ttgtgctatg cagacggcga gcgtcgttac atcatcgctc ctcgcggtat tcaagtcggt
     3241 gccgtattgg tttccggtgc tgaagctgcc atcaaagtag gcaacaccct gccgatccgc
     3301 aacattcccg ttggtacgac tatccactgt atcgaaatga aacccggtaa aggtgctcaa
     3361 atcgcacgtt ctgccggtgc ttctgcggta ttgctggcta aagaaggcgc gtacgctcaa
     3421 gtccgtctgc gctctggcga agttcgtaaa atcaacgtaa attgccgtgc gaccatcggt
     3481 gaagttggta acgaagagca aagcctgaaa aaaatcggta aagccggtgc caaccgttgg
     3541 cgcggtattc gtccgaccgt acgcggtgtt gtcatgaatc ctgtcgatca cccgcatggt
     3601 ggtggtgaag gccgtacggg cgaggcccgc gaaccggtca gcccatgggg tactcctgct
     3661 aaaggctacc gcactcgtaa taacaaacgc acggataaca tgattgttcg tcgccgttac
     3721 tcaaataaag gttaatttag tatggctcgt tcattgaaaa aaggcccata tgtagacctg
     3781 catttgctga aaaaagtaga tgctgctcgc gcaagcaacg acaaacgccc gattaaaacc
     3841 tggtctcgtc gttctaccat tctgcctgat tttatcggtc tgaccattgc tgtgcacaac
     3901 ggccgcaccc atgtgcctgt gtttatcagc gacaatatgg ttggtcataa attaggcgaa
     3961 ttctcattga cccgtacctt taaaggccac ttggccgata aaaaggctaa aaagaaataa
     4021 ggtgaatcat gagagtaaat gcacaacata aaaatgcccg tatctctgct caaaaggctc
     4081 gtttggtagc tgatttgatt cgtggtaaag acgttgccca agctttgaat attttggctt
     4141 tcagtcctaa aaaaggtgcc gagctgatta aaaaagtatt ggagtcagct attgctaatg
     4201 ccgagcacaa taacggtgcg gacattgatg aactgaaagt ggtaactatc tttgttgaca
     4261 aaggcccaag cttgaaacgt tttcaagctc gcgccaaagg tcgcggtaac cgcatcgaaa
     4321 aacaaacttg tcatatcaat gtgacagtgg gtaactaagg aaaagctatg ggacaaaaga
     4381 ttaaccctac aggctttcgc ctggcggtaa ctaaagactg ggcttcaaaa tggtttgcta
     4441 aaagcaccga cttttctact gttttgaagc aggatatcga tgttcgcaat tatttgcgtc
     4501 aaaaattggc caatgcttcg gttggtcgag tggttattga acgccctgca aaatctgcac
     4561 gcattaccat tcactccgct cgtccgggtg tggttatcgg taaaaaaggt gaggatatcg
     4621 aggttttgaa acgtgacttg caagtcttga tgggtgtacc tgttcatgta aatattgaag
     4681 agattcgccg tcctgagttg gatgctcaaa ttattgctga cggtattgcc cagcagttgg
     4741 aaaagcgcgt tcaattccgt cgtgctatga aacgagcaat gcaaaatgca atgcgttctg
     4801 gtgctaaagg cattaagatt atgacttcag gccgtctgaa tggtgcggat attgcccgta
     4861 gcgaatggta tcgtgaaggt cgtgtgccac tgcatacttt acgtgcaaat gtagattatg
     4921 caaccagcga agcgcacacc acatatggtg tattgggtct gaaagtttgg gtttatacgg
     4981 aaggcaatat taaatcttcc aaacctgaac atgagagtaa acaaagaaag gcaggtagac
     5041 gtaatgctgc agccaactag actgaaatac cgtaagcaac aaaagggtcg caatactggc
     5101 atcgctactc gcggtaataa ggtaagtttc ggtgagttcg gcttgaaagc cgtaggtcgt
     5161 ggtcgtttga ctgcccgtca aatcgaagct gctcgtcgtg caatgacccg tcatatcaaa
     5221 cgtggtggtc gtatttggat tcgtgtattc cctgataaac cgattactga aaagcctatt
     5281 caagttcgta tgggtggcgg taaaggtaac gtggaatatt acattgccga aattaaacca
     5341 ggtaaagtgt tgtatgaaat ggatggcgtt ccagaggaac tggctcgtga agcattcgag
     5401 ttggctgctg ccaaattgcc tattcctaca acctttgtag taagacaggt gggtcaataa
     5461 tgaaagcaaa tgaattgaaa gacaaatccg ttgagcagtt gaatgcagat ttgttggact
     5521 tgttgaaagc tcagtttggc ttacgtatgc aaaacgctac cggtcaatta ggcaaaccaa
     5581 gtgaattgaa acgtgtacgt cgcgatattg ctcgtattaa aaccgtttta actgaaaaag
     5641 gtgctaagta atgagcgaaa ctaaaaatgt tcgtactttg caaggcaaag tagtaagcga
     5701 caaaatggat aaaaccgtaa cagtattggt tgagcgtaaa gtaaaacatc cgctgtatgg
     5761 taagattatt cggttatcta ctaaaatcca tgcccatgat gaaaataatc aatatggaat
     5821 tggtgatgtg gttgttatat cggaatcccg tccattgtca aaaactaaat cttgggttgt
     5881 cagtgagctg gttgagaaag cacgttctat ttaagaatta aagcaacgtg cttggaatgg
     5941 gaaacgaagt attgcagcaa atttaatttg cgtgtaaact tcgtttcctg tctttcagtt
     6001 tcttctggaa gtttcttccc tttcggggtc caagactggt ttacttgaac cgcaaggttt
     6061 catttaataa gcagcggctt tgctgtaagt tatctgaaag tggtaaatta agttggttaa
     6121 tttaaaggta ataacatgat tcaaatgcag accatcttag atgtggctga taactctggt
     6181 gcgcgtcgcg taatgtgtat caaggtattg ggcggatcta agcgtcgcta cgcttctgtt
     6241 ggcgatatta ttaaagtggc agttaaagat gcggctccgc gtggccgtgt caaaaaaggc
     6301 gatgtatata atgcggtagt tgttcgtact gctaagggtg tacgtcgtcc tgatggtgcg
     6361 ttaattaaat tcgataacaa tgccgccgtg ttactgaata ataaacttga acctttgggt
     6421 actcgtatct ttggtccggt aacccgtgaa ttgcgtactg agcgatttat gaaaatcgtt
     6481 tcattggcac ctgaagtatt ataaggaatg gcacgatgaa taaaatcatt aaaggcgata
     6541 gggttgtagt aattgctggt aaggataaag gtaagcaggg tcaagtagtt cgagtgttgg
     6601 gtgataaagt tgttgttgag ggcgttaatg ttgtaaaacg ccatcaaaaa cctaatccaa
     6661 tgcgtggcat tgagggcggt attattacta aagaaatgcc tttggatatt tctaatatcg
     6721 caatcctgaa tccggaaact aataaagcgg accgtgttgg tattaagctg attgaaaatg
     6781 aaggcaaagt taaacgcgtt cgtttcttca aatcaaatgg ctctatcatt ggggcataag
     6841 gagataacat ggctcggttg agagagtttt ataaagagac agttgttcct gaattggtta
     6901 aacaatttgg ttacaaatca gtaatggaag tcccgcgtat tgaaaaaatc accttgaata
     6961 tgggtgtggg tgaggctgtt gctgacaaaa aagttatgga acatgctgtt tccgatttag
     7021 agaaaattgc cggtcaaaaa ccggttgtta ctgttgcccg taaatctatc gcaggtttta
     7081 aaatccgtga taactatccg gttggttgca aagtaacatt gcgtcgtgat caaatgtttg
     7141 aattcttgga tcgtttgatt actattgcat tacctcgcgt acgtgacttc cgtggtgtga
     7201 gcggtaaatc atttgatggc cgtggcaatt acaatatggg tgttcgtgag caaattattt
     7261 ttccggaaat tgaatacgat aaaattgatg ctttgcgtgg tttgaatatt actattacta
     7321 ctacagcaaa aaccgatgag gaagcgaaag ctttattgtc attgtttaaa tttccgttca
     7381 aaggataatc atggctaaga aagcacttat taatcgtaat ctgaaacgtc aagctttggc
     7441 taaaaaatat gcggctaaac gcgcggcaat taaagcggta atcaatgatt cgaatgcaac
     7501 tgaggaagag cgttttgagg ctcgtttgag gtttcaatcc attcctcgta atgcggcacc
     7561 tgtgcgtcaa cgtcgtcgtt gtgctttgac aggtcgccct cgtggtactt tccgtaaatt
     7621 tggtttgggt cgtattaaaa tccgtgaaat cgccatgcgt ggcgaaattc cgggtgttgt
     7681 taaagccagc tggtaatagg agtaattaag aatgagtatg catgatccta tttccgatat
     7741 gttgactcgt atccgcaatg cgcaacgtgc taataaagca gcggttgcaa tgccttcttc
     7801 aaaattaaag tgtgctattg caaaggtatt gaaagaagaa ggatatattg aggacttcgc
     7861 agtttcatct gacgtaaagt ctatattgga aattcaatta aaatactatg caggtcgtcc
     7921 tgtaattgaa caaatcaagc gtgtatctcg ccccggtttg cgtatttata aagcgtctag
     7981 tgagattcca agtgttatga atggcttggg tattgctatt gttagtactt ctaaaggtgt
     8041 aatgactgat cgtaaagcac gttctcaagg tgttggtggt gagttgttat gcattgtagc
     8101 ctagtggagg aaaagaaatg tcacgtgtcg caaaaaaccc agtgactgtt cccgctggtg
     8161 tagaagtaaa atttggaaca gaggcattag ttattaaggg taagaacggt gaattgtctt
     8221 ttcctttgca ttctgatgta gccattgaat ttaatgatgg caaattggct tttgttgcga
     8281 ataacagcag taaacaagca aatgcaatgt ctggtactgc tcgcgcatta gtcagcaata
     8341 tggttaaagg tgtttcagaa ggttttgaga aaagattgca attgataggt gtgggttatc
     8401 gtgctcaagc acaaggtaaa atcttgaatc tgtctttggg tttttctcat ccgatcgtat
     8461 atgaaatgcc tgaaggtgtc tccgttcaaa ctcctagcca aacagagatt gttttaaccg
     8521 gctcggataa acaagttgtt ggtcaagttg ctgctgagat tcgtgcgttc cgtgctcctg
     8581 agccttataa aggtaaaggt gttcgctatg taggagaagt agtggtaatg aaagaagcca
     8641 agaaaaaata attgaggttc actaatggat aaacatacaa cccgactccg tcgtgcacgc
     8701 aaaacccgtg ctcgtattgc ggacttgaaa atggtaagat tatgtgtgtt ccgaagcaat
     8761 aatcatattt atgctcaagt aattagtgct gaaggtgata aagtattggc tcaagcctct
     8821 acattggaag ctgaggtgcg cggtagtctg aaatctggaa gcaatgttga agcagctgca
     8881 atagttggta aacgtatcgc tgagaaggct aaagcagcag gtgtagaaaa ggttgctttt
     8941 gatcgttcag gtttccaata tcacggtcgt gtgaaggctt tggctgaagc tgctcgtgaa
     9001 aatggtttaa gcttctaaat atttggagac tttcagatgg caaaacatga aattgaagaa
     9061 cgcggtgacg gtctgattga aaagatggtc gcagttaacc gtgtaaccaa agtagttaaa
     9121 ggtggtcgca ttatggcttt ctctgcgcta actgttgttg gtgatggaga tggtcgtatt
     9181 ggtatgggta aaggtaagtc aaaagaagtg cctgttgcag tccaaaaggc gatggatcaa
     9241 gcacgacgct ctatgattaa ggtaccatta aaaaatggta cgattcatca tgaggttatt
     9301 ggtcgacatg gtgctactaa agtatttatg cagcctgcta aagagggtag tggcgtaaaa
     9361 gccggtggac ctatgcgttt ggtttttgat gctatgggca ttcataatat ctccgccaaa
     9421 gtgcacggat ctactaaccc atataatatc gtacgtgcaa cattagatgg tttgtctaag
     9481 ttgcatactc ctgctgatat cgcagccaaa cgtggcttga cagtggaaga cattttggga
     9541 gttaaccatg gctgaacaaa aaaagattag ggttacattg gttaaaagcc tgattggtac
     9601 aattgaatct catcgtgcat gtgcacgcgg tttaggtttg cgtcgtcgcg agcatacggt
     9661 agaggtttta gatacccctg aaaaccgtgg tatgattaat aaaatcagct acttgttgaa
     9721 agtggagtct tgatatgttt ttgaatacaa ttcaacctgc tgttggtgct acgcatgctg
     9781 gtcgtcgtgt tggacgcggt attggtagtg gtcttggcaa aacgggtggt cgtggtcata
     9841 aaggtcaaaa gagccggtct ggtgggtttc ataaggtggg tttcgagggt ggtcaaatgc
     9901 ccttgcaacg acgcctccct aaaagaggtt ttaaatcttt aacagcatca gctaatgcac
     9961 agcttcgttt aagtgaactg gaatcaattg ctgttaatga gattgatatt ttggtcttaa
    10021 agcaagcggg tctgattgca tctacagtct ctaatgttaa agttattgct tctggtgaaa
    10081 tttctaaggc agttgctttg aagggtatta aagttaccaa aggtgcgaga gctgctatcg
    10141 aggctgttgg tggtaagatt gaaatgtaag gtttaatatt gtggctaatc aacaaacgtc
    10201 atcaggttca tccaaatttg gagatcttaa gaaacgtctt ttgtttctat ttggagcatt
    10261 gattgttttt cgaattggtg cccatatacc cgtacctgga gttgatgctg ttgctttagc
    10321 taaattatac gaaagcgctg gaaacggcat cctgggaata ttgaatatgt tttccggtgg
    10381 gtcgttagag cgctttagta tatttgcaat aggaattatg ccatatattt cagcttctat
    10441 tattgtacag ctcgcttctg aaattttgcc atcattgaag gctttaaaaa aagaagggga
    10501 ggctggtaga aaggtaatta cgaaatatac taggtatggt actgttttgt tagcaattct
    10561 tcaaagtcta ggtgttgcat ctttcgtatt tcagcaagga attgttgtaa caagttcatt
    10621 tgagtttcat gtttccacgg tagtttcttt ggtaacggga accatgtttc ttatgtggct
    10681 tggggagcaa attactgaaa ggggtatcgg gaacggtatt tctttaatca ttacggcagg
    10741 tattgcttca ggtattcctt cgggtattgc aaagctggtt acactgacga accaaggttc
    10801 tatgagcatg cttacggcgt tgtttattgt atttggtgcc ttattattaa tttatttggt
    10861 tgtatacttt gaaagtgcac agcggaagat tcctattcat tatgcaaaac gccagtttaa
    10921 tggtagggcg ggtagtcaaa atacgcatat gcctttcaag ttgaatatgg ctggtgttat
    10981 tcccccaatt tttgcttcca gtattattct atttccatct actcttttag gttggtttgg
    11041 ttcggctgat acaaatagtg ttttgcacaa aatagctgga ttgttacaac acggtcaatt
    11101 gctgtatatg gctttatttg cagcgacagt tattttcttt tgttattttt atacggcttt
    11161 ggtttttagc cctaaagaaa tggcagagaa tttaaaaaag agtggtgctt ttgttcctgg
    11221 gattagacct ggtgagcaga cctctaggta tttagaaaaa gttgtattac gtttgacatt
    11281 gtttggagct ctttatatta caactatttg tttaattcca gagttcttaa ctacggtttt
    11341 aaatgtacct ttttatttgg gtggcacgtc tttgttgatt ctagttgttg taacgatgga
    11401 ttttagtaca caaataaatt cgtataggct tactcaacag tatgataagt taatgactcg
    11461 ttcagaaatg aaatcatttt ctcggaaata gaattatggc gaaagaagat actatccaaa
    11521 tgcaaggtga aattcttgaa actttaccta atgcaacatt taaagtaaaa cttgagaatg
    11581 accatattgt attgggtcat atttctggga agatgcggat gcattacatt cgtatttctc
    11641 cgggagataa ggtcacagta gagctgacac cttatgatct aactagggct cgaatcgttt
    11701 tcagagcaag ataaaccaat aaaaggaaaa taaaatgcgt gtacaaccat ctgttaagaa
    11761 aatttgccga aattgcaaga ttattcgtcg aaatcgtgta gttcgtgtaa tttgtactga
    11821 tctccgtcac aaacagcgtc aaggttaatg gaatatttct tttaatgtga ttctgtgata
    11881 tagtgacaca ctttgcccta aaaaggaaaa aatatggctc gtattgcagg ggtaaatatc
    11941 cctaataacg cacacatcgt aattggtctt caggctattt acggtattgg tgctactcgt
    12001 gctaaattga tttgtgaggc tgcaaatatt gcgcctgata ctaaagcaaa agatttggac
    12061 gagactcaat tagatgcttt gcgtgaccaa gttgccaagt atgaagtaga aggtgatttg
    12121 cgtcgtgagg taactatgag tatcaagcga ttgatggaca tgggctgcta tcgtggcttc
    12181 cgtcatcgtc gcggcttacc atgccgcggt caacgcactc gtacaaatgc gcgtacccgc
    12241 aaaggtccgc gtaaagcgat tgctggtaag aaataaattt taaggaattt tattaatggc
    12301 taaagcaaac acagcttcac gtgtacgtaa aaaagtacgt aaaaccgtga gtgagggtat
    12361 tgtgcacgtt catgcatctt tcaacaatac catcattaca atcactgacc gtcaaggcaa
    12421 tgcgttgtct tgggctacct ctggcggcgc tggttttaaa ggttctcgta aaagtacacc
    12481 atttgcagca caagttgcag cagaagcagc tggtaaagtt gcccaagagt atggcgttaa
    12541 aaatttagag gttcgtatta aaggtccagg tccaggtcgt gaatcctctg tacgtgcttt
    12601 gaatgctctt ggtttcaaga ttaccagcat tactgacgtt accccgttgc ctcataacgg
    12661 ttgccgtccg cctaaaaaac gtcgtattta atattggagt gatttgaaac atggcacgtt
    12721 atattggccc taaatgtaag ttggcacgtc gcgaaggtac ggatttgttt ttgaagagtg
    12781 cgcgccgctc tttggattct aaatgtaaaa ttgattccgc tcccggtcag catggtgcaa
    12841 aaaaaccgcg tttgtcagac tatggtttgc agttgcgtga aaaacaaaaa atccgccgta
    12901 tttatggcgt attagaacgt cagttccgtc gttatttcgc agaagctgat cgtcgtaaag
    12961 gttctaccgg cgagttgctg ttgcagttgc tggaatctcg tttggataat gtcgtttatc
    13021 gtatgggttt cggttctacc cgagctgaag caagacagct tgtttctcat aaggcgatag
    13081 ttgtgaatgg acaagttgtc aatattcctt ctttccaagt gaaagctggt gatgttgtct
    13141 cagttcgtga aaaagccaaa aaacaggtac gtattcaaga agcattgggt ttggcaactc
    13201 aaatcggctt gccgggttgg gtttctgtag atgcggataa acttgagggt gtgttcaaaa
    13261 acatgccgga tcgctcggaa ttgaccggtg atattaatga acagctggtg gtagagttct
    13321 actctaaata atgctagctc agtgagggac agttaaatgc agaatagcac aaccgaattt
    13381 ttgaaacctc gtcaaattga tgtaaatact ttttctgcaa ctcgtgcaaa agtatctatg
    13441 cagccatttg aacgtggttt cggtcatacc ttaggtaatg ctttgcgccg tatcttactg
    13501 tcatccatga atggttttgc tcctactgaa gtagctattg ccggtgtatt acacgaatat
    13561 tctactgttg atggtattca ggaagatgtt gttgacattt tgctgaatat taaaggtatt
    13621 gtgtttaaac tccatggtcg tagccaagtt caacttgtgt tgaagaaatc aggttcaggt
    13681 gtcgtatctg ccggtgatat tgagttgccg catgatgtag aaattctgaa tcctggtcat
    13741 gtcatttgtc atttggctga taacggtcaa attgagatgg aaattaaagt agagcaaggt
    13801 cgtggttatc aatctgtttc aggtcgtcag gtagttcgtg atgagaaccg tcagattggt
    13861 gcaatccagt tggatgcgag cttttcgccc atcagccgtg ttagctttga ggttgaacct
    13921 gcacgtgtag agcagcggac ggatcttgat aagttggttt tggatatcga aaccgacggt
    13981 tctattgatc ctgaggaagc tgtacgcagt gcggcacgta ttttgattga tcagatgtct
    14041 atttttgctg atttgcaggg tacgcctgtg gaggaggttg aagaaaaagc acctcctatc
    14101 gaccctgttc ttttgcgtcc ggtggatgat ctggaattga cagtacgttc agctaattgt
    14161 ttgaaagctg aggatattta ttatattggc gatttgattc aacgcactga aaccgagctt
    14221 cttaaaacgc cgaatttggg acgtaaatct ttgaatgaga ttaaggaagt attggcatct
    14281 aaaggtttga cactgggttc taagttggaa gcatggccac ctgtaggctt ggaaaagcct
    14341 taatgaagaa ttaaaggata attgatatgc gtcatcgtaa tggcaatcgc aaattaaacc
    14401 gtaccagcag tcatcgtgct gcaatgctgc gtaatatggc gaattcatta ttgactcacg
    14461 aaactattgt aacaactctg cctaaggcca aggaattgcg ccgtgtagta gagccgttga
    14521 ttacattggg taaaaagccg tcattggcaa accgccgttt ggcatttgac cgtactcgcg
    14581 accgtgatgt tgtagtaaaa ctgtttggcg atttgggtcc tcgttttact gctcgtaacg
    14641 gtggttatgt tcgggtgttg aaatacggat tccgtaaagg tgataatgca cctctggcac
    14701 tggttgaatt ggttgacaaa ccggctgctg agtaatttta gtcatataac gccatctgcc
    14761 gaaaagcagg tggcgttatt tttgcaatat ctgataggta atagggtatt ggctatcatg
    14821 tttaaaatat taattgaata gctaaggttt gcgcggtaaa cttacatcat taaaaaattc
    14881 tatgatggtt tatataatga atgctttcga tataaagtcg acaaagatgg acgtattgtc
    14941 tatatctttg catacgtcag acttgtttga tttggaagat gtgctggtca aattgggcaa
    15001 gaagtttcaa gagtctggtg ttgttccatt tgtgctggat gttcaagagt ttgattatcc
    15061 cgagtctttg gatcttgctg cattggtttc gttgttttca aggcatggta tgcaaatttt
    15121 gggtctgaag cattctaatg aacgttgggc tgctgcggct atgaagtatc atttgctgtt
    15181 ttgtctgtct cattcggaaa atgttaaaga actgggtcag gttgaggtgc agaacacgga
    15241 ggatggtcag aaagcaagga aaacagtatt gattacatcc cctgtccgta ccggtcagca
    15301 ggtttatgcc gaagatggcg atttgattgt tacgggggcg gtcagtcagg gggcggaatt
    15361 gattgcggat ggcaatatac atatttatgc gccgatgagg gggcgtgctt tggccggcgc
    15421 caagggtgat acttctgccc gcatatttat ccactccatg caggcagaac tggtttctgt
    15481 ggcgggtatt taccgtaatt ttgaacagga tttgccggat catctgcaca agcagccggt
    15541 acagatattg ttgcaggata atcggttggt tatcagtgca attggctcag agtaattgtt
    15601 tgatatttta aaaaggaaat attgtggcaa aaattattgt agtaacttca ggtaagggcg
    15661 gtgtcggtaa aacgactacc agtgccagta ttgcgacagg tttggcatta cgcggatata
    15721 aaactgcggt aattgatttt gatgtgggtt tgcgtaacct cgacctcatt atgggttgcg
    15781 agcgtcgtgt cgtttatgac ctgatcaatg tcattcaggg ggaggcgacg ctcaaccagg
    15841 ctttgattaa agataaaaat tgtgaaaacc tgtttatttt gccggcttcc cagactcggg
    15901 ataaagacgc tttgacacgc gagggcgtag aaaaagtgat gcaggagctg tccggcaaga
    15961 aaatgggctt tgagtatatt atttgcgact ctcctgccgg tattgagcag ggtgcattga
    16021 tggcgttgta ttttgctgat gaagccattg taacgaccaa tcctgaggtt tccagtgtgc
    16081 gtgactccga caggattttg ggaattttgc aaagcaaatc ccataaggca gagcaaggcg
    16141 gttcggttaa agaacatctg ttgattacgc gttattctcc cgaacgtgtg gcaaaaggcg
    16201 aaatgctgtc tgtacaggat atttgcgata ttctgcatat tcctttgctg ggtgtgattc
    16261 ctgaatccca aaacgtcttg caggcatcca attccggaga accggtcatc catcaggaca
    16321 gcgtggcggc ttccgaggca tataaggacg ttattgcccg tcttttgggc gagaaccgtg
    16381 aaatgcgttt cttggaagct gagaaaaaaa gcttcttcaa acgtctgttt ggaggataag
    16441 gtatgtcatt aatcgaattt ttattcggca gaaagcagaa aacggcaacc gttgcccgcg
    16501 accgccttca aatcatcatt gcccaagagc gcgcccaaga aggtcaggct ccggattacc
    16561 tgccgacttt acgtaaagag ttgatggaag tcctgtccaa atatgtgaat gtttcattag
    16621 acaatatccg tatttcccaa gaaaagcagg atggtatgga tgtgcttgag ttgaacatta
    16681 ctttgccgga acagaaaaag gtataggaca tgaccttaac cgaattgcgg tacatcgtcg
    16741 cagtcgccca agaacgtcat ttcggcaggg cggcgcggcg ttgttttgtc agccagccca
    16801 ctttgtctat tgccattaag aaattggaag aagagcttgc cgtctctttg tttgaccgga
    16861 gcagcaacga tattattacg accgaggcgg gggaacgtat cgttgcacag gcgcgtaagg
    16921 tgttggaaga ggcagagctt atcaggcatt tggcaaatga agaacaaaac gagctggagg
    16981 gtgcgttcaa actcgggctg atttttacgg ttgcgccgta cctgctgccg aaactgattg
    17041 tctcgttgcg ccgtactgca ccgaaaatgc ctttgatgtt ggaagagaat tacacgcata
    17101 ctttgaccga gtcgctcaaa cgcggggacg ttgacgcgat tatcgttgcc gaaccgtttc
    17161 aagagccggg cattgttacc gaacccttgt atgacgaacc gtttttcgtg attgtcccga
    17221 aagggcattc atttgaggaa ctggatgccg tttcgccacg gatgctgggt gaggagcagg
    17281 ttttgctgct gacggaaggc aactgtatgc gggatcaggt actctcaagc tgttccgaat
    17341 tggcggcgaa acaacgtata caggggttga ccaatacatt gcagggcagc tcgatcaata
    17401 caatccgcca tatggtcgcc agcggtttgg caatcagcgt gttaccggca accgcgctga
    17461 ccgaaaacga tcatatgctg ttcagcatta ttccgtttga aggtacgccg ccaagccggc
    17521 gggtcgtatt ggcgtaccgc cgcaattttg tccgtccgaa ggcgttgtcg gcgatgaagg
    17581 cggcgattat gcagtcgcag cttcacgggg taagttttat ccgcgactag gcgcaggcat
    17641 tgttttcaaa acgccatttc cctgagccga caacacggta tgccaagata ttgccgtcat
    17701 catcgatttt gagtatagca tcgccacgga aactgccgtc ctgaagatat tcgacttttg
    17761 catcactgtg aatgttttca tcagtgccga tgcaatgcca tgtatagtgg attaaca
//
LOCUS       DAWFAN010000028        16779 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWFAN010000028 DAWFAN010000000
VERSION     DAWFAN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16779
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.028"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(177..1346)
                     /locus_tag="WGO65_000662"
     CDS             complement(177..1346)
                     /locus_tag="WGO65_000662"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ0326988.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1520..1630)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1683..2579
                     /locus_tag="WGO65_000663"
     CDS             1683..2579
                     /locus_tag="WGO65_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ0326989.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLANVKGVKTLFFCCERLPDGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2623..3687)
                     /gene="tsaD"
                     /locus_tag="WGO65_000664"
     CDS             complement(2623..3687)
                     /gene="tsaD"
                     /locus_tag="WGO65_000664"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ0326990.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVK"
     gene            complement(3803..4990)
                     /gene="ccsB"
                     /locus_tag="WGO65_000665"
     CDS             complement(3803..4990)
                     /gene="ccsB"
                     /locus_tag="WGO65_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ0326991.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLCAEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4983..6935)
                     /locus_tag="WGO65_000666"
     CDS             complement(4983..6935)
                     /locus_tag="WGO65_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ0326992.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVNIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7212..7835)
                     /locus_tag="WGO65_000667"
     CDS             complement(7212..7835)
                     /locus_tag="WGO65_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ0326993.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            8041..8670
                     /locus_tag="WGO65_000668"
     CDS             8041..8670
                     /locus_tag="WGO65_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ0326994.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8791..11187)
                     /locus_tag="WGO65_000669"
     CDS             complement(8791..11187)
                     /locus_tag="WGO65_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ0326995.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPKVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11339..12454
                     /locus_tag="WGO65_000670"
     CDS             11339..12454
                     /locus_tag="WGO65_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ0326996.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12457..13056
                     /locus_tag="WGO65_000671"
     CDS             12457..13056
                     /locus_tag="WGO65_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ0326997.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13057..13704
                     /locus_tag="WGO65_000672"
     CDS             13057..13704
                     /locus_tag="WGO65_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ0326998.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13722..14267
                     /locus_tag="WGO65_000673"
     CDS             13722..14267
                     /locus_tag="WGO65_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ0326999.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14286..16547
                     /locus_tag="WGO65_000674"
     CDS             14286..16547
                     /locus_tag="WGO65_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ0327000.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQTNIDFRKDG
                     KNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLK
                     RLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISL
                     DFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQ
                     QGNIVNIAPRDELLAKDKALLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNAD
                     TTGNRNTLISGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVE
                     AADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINLPITAAANS
                     ISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTSIAN
                     GGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLN
                     TQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFIT
                     PRIMGTAGNSLRY"
BASE COUNT         4499 a         4762 c         3824 g         3694 t
ORIGIN      
        1 ctcatggaaa atcccctaaa tgtcttggtg ggaatttagg ggattttggg gaattttgca
       61 aaggtctcta tctgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      121 tatataaaaa tgccgtctga actgttcaga cggcattttt gattttcaaa ccggaattac
      181 agccccgctg ccgccctcaa tgcagcagct ttgtcggtgc gctcccaagt gaactcaggt
      241 tcttcgcggc cgaaatgtcc gtaagcggcg gatttactgt aaatcgggcg caagaggtcg
      301 agcatttgga cgatgccttt ggggcgcagg tcgaaatgtt cgcgaactaa ggtaatcagt
      361 ttttcttcgc tgattttgcc tgtgccgaaa gtatcgatgg aaatcgaagt cggttcggca
      421 acgccgatgg cgtaggaaac ttggatttgg cattgggttg ccaatcctgc ggcgacgatg
      481 tttttcgcca catagcggca ggcgtaggcg gcggaacggt caaccttgga cgggtctttg
      541 ccggaaaacg cgccgccgcc gtgcggagcc gcgccgccgt aggtatcgac gatgatttta
      601 cggccggtca aaccgcagtc gccttgcggg ccgccgatga cgaagcggcc ggtcgggttg
      661 atcaggtatt tggtttcgtc ggtcagcagt tcagacggca ggacgggctt gataatctgc
      721 tcgatgacgg cgtttttcag ttcttcgtaa gcgatggacg gatcgtgctg ggtagagagg
      781 acgacggtat caatgcgttt taccttaccg gtttcgctgt cgtaaaccac ggtcaactgc
      841 gcttttgcgt cgggacgcag ccacggcagg cggccgtctt tacgcaattc gctttggcgt
      901 tgcatcaggc ggtggctgta atagatggca aacggcatca gggtcggggt ttcgtcgcag
      961 gcataaccga acatcaaacc ttggtcgcct gcgccttggt tcaagtcgat accttcgcct
     1021 tcgtttacgc cttgggcgat gtcgggggat tgttggtcgt agtacacgcc gaccgcgcag
     1081 ccgttggcat caaagcccag ctcggaggag ttgtagccga tgcgtttgat ggtttcgcgt
     1141 gcgactttga tgtagtccac ttgggcggtg gtggtgattt cgcctgccaa tacgcacaag
     1201 cctgtgttga ccaaagtttc tgcggcgaca cgcgctttgg ggtcttgcgc caagatggca
     1261 tccaaaatcg catcggacac ttggtcggca actttgtccg gatggccttc ggataccgat
     1321 tcggaagtaa acagatattc gctcattgct attctttcat taaaaacagg ctgctgtgtt
     1381 ttcagacggc cttttatttc ggacagcgga aatcataaca aaaactccgc tttttctcta
     1441 tacaagtgag aaacattcgc acttgtctgc tgtttcggaa tcctgttgcg cgtttcttac
     1501 aggcaaaaaa attcccgcat taagcgggtt tggattgctc tggtgagcca catcggcttt
     1561 tcaaccgtcc accttacttt tccttttgaa aagcaggttg gcatggaatt cccaactctt
     1621 aatgcagcgc ggatggtagc agaaaagcct ttccgccgca atacttccct ttttcagacg
     1681 gcatgtttcg tttacaattc gggctgtttc cccctttgcg aaccgccatg cacatcctgt
     1741 tgaccgccct gctcaaatgc ctctccctgc tgccgctttc ctgtctgcac acgctgggaa
     1801 accggctcgg acatctggcg ttttaccttt taaaggaaga ccgcgcgcgc atcgtcgcca
     1861 atatgcgtca ggcaggcatg aatcccgacc ccaaaacggt caaagccgtt tttgcggaaa
     1921 cggcaaaagg cggtttggaa cttgcccccg cgtttttcag aaaaccggaa gacatagaaa
     1981 caatgttcaa agcggtacac ggctgggagc acgtgcagca ggctttggac aaacacgaag
     2041 ggctgctgtt catcacgccg cacatcggca gctacgattt gggcggacgc tacatcagcc
     2101 aacggctgcc attcccctta accgccatgt ataaaccgcc gaaaatcaaa gcgatagaca
     2161 aaatcatgca ggcgggcagg gttcgcggca aaggaaaaac cgcgcctacc agcatacaag
     2221 gggtcaaaca aatcatcaaa gccctgcgtt cgggcgaagc aaccatcgtc ctgcccgacc
     2281 acgtcccctc ccctcaagaa ggtggggaag gcgtatgggt ggatttcttc ggcaaacctg
     2341 cctataccat gacgctggcg gcaaaactgg caaacgtcaa aggcgtgaaa accctgtttt
     2401 tctgctgcga acgcctgcct gacgggcagg gtttcgattt gcacatccgc cccgtccaag
     2461 gggaattgaa cggcgacaaa gcccatgatg ccgccgtgtt caaccgcaat gccgaatatt
     2521 ggatacgccg ttttccgacg cagtatctgt ttatgtacaa ccgctacaaa atgccgtaac
     2581 gaaaataaaa atgccgtctg aacaatttca gacggcattt tgtcatttga cgatttccga
     2641 cagcggccag cgcggacgga cattgaacgc accgacctcc ctgcccttgc ccaggtgcat
     2701 cgcaccggca aaggcaatca tggcaccgtt gtccgtgcag tatgccgtcg gcgggaaaaa
     2761 cacgctgact ttttcggacg gatgtttcgg cttgcctttg ggggtcggga tttgcaccgt
     2821 catgttgccg aaagtttcac ggagcttgcg gtttgcaccg accccgccgg cgaccactac
     2881 ggttctgaac cctgtctgca acagggcttt tttcactttc gccgccaaca catcgactac
     2941 cgcatcttga aacgcacggc agatgtcgtt gcgtgtctgc tcaggaatgt catccgcccc
     3001 gttttccgcg cgcactttct cgacggcggt caatacggcg gttttcaaac ctgaaaaact
     3061 catctgcaaa tcgtcggaat gaatcattgg gcgcggaaaa acaaacgctt cgggcctgcc
     3121 cgattccgca agttccgaca gtttcgcacc gcccggatac ggcaagccca gcagtttcgc
     3181 cgttttgtcg aatgcctcgc ccgccgcatc atcgacgctc tcgcccaaaa gcgcgtagtc
     3241 gcctataccc ctgaccgcca taatctgcgt atgcccgccc gaaaccagca gcgcgacaaa
     3301 aggaaagtcg ggtttttcct ccgccaacag cggcgacagc agatgtcctt ccaaatgatg
     3361 gacggggatg acgggcttgc ccaatgccaa agccagtgca ttggcgtagc tcgaacccgc
     3421 cagcagcgca ccgcccaaac cgggtccctg cgtaaaggca accgcgtcaa tgtcgccata
     3481 cgatgcgccc gcctgcgcca aacagccttc cgtcaacgga acaaggcggc ggatatggtc
     3541 gcggcttgcc aattccggca caaccccgcc atattcggcg tgcattgcca tctgcgtgtg
     3601 cagacagtgc gcccgcaatc ctcgttccgt atcgtaaagc gcaacgccgg tttcgtcgca
     3661 agaagattcg attcctaata ccaacatggt ctgatgccgt taaaaactga aaaacgtatt
     3721 ctagcggatt tcggcacgac tgccgtatcc caaaaacgga acatgccgtc tgaagccctt
     3781 cagacggcat cgtcgcaccg tatcaaagcg ttccgtaaga atgcagcccg ctcaaaaaca
     3841 tattcacgcc gataaaggcg aatgcggtta cgaacaaacc gataatcgcc caccacgcca
     3901 gcactttgcc gcgccaaccg gcaaccagcc gcaagtgcag ccaaacggcg taattgagcc
     3961 agacgatgaa cgcccacgtc tctttcgggt cccaactcca atagcgtccc caagcatccg
     4021 ccgcccacag cgcacccaaa atggtggcaa tggtaaagaa cagaaagccg acggcaatcg
     4081 ccttatacat gacttcctca atcaacacgg attgcggcag ccacgatttc ccgccttttc
     4141 cttccgcaca cagggaaacc agttcggcaa taccgagcat cgcggaaatg caaaacgcgc
     4201 cgtaaccgat aaagtttgcc ggaacgtgga ttttcatcca ccaagattgc aatgccggaa
     4261 tcagcggctg gatggtatgc gcctcgcggg acacgctgta ccacaagaca aatccaacca
     4321 cgaccgccat aaagccgaac acgaagccgc ccaatttctg tatggcgaac ttaccttcat
     4381 aataaagata catcagcgcg gtaatcacca aaaacaggat gaacacttca tacaggttgg
     4441 aaaccggaat atgccccgca tcgggacgga gcagatagct ttcgtgccaa cgtaccagca
     4501 gaccggtaaa gcctgctacg gcagacaccc atgcaaacac ggttcccata cccaacagcg
     4561 tgttggtcgg cacatttttt acgcttgcca aaaccgcgcc cgaaatatag gcgaacaggg
     4621 cgaagaagac aaaggcgcac tgccacatga tcgccgactg gctgctgagg aaataccgca
     4681 acaggaaaat ctctgccgat ttgatgttgc cgccatacaa accgacggcg gcataggcaa
     4741 gcaatacgct taaaggaaca aaccagcgca tcggtttgaa aaaccaaccc aaaaacacgg
     4801 caataccggc acttgcccac aacatgaccg tttcgtaaat gtccatatgc ataccggaac
     4861 gggtctgtac gaaaaccgta gccgcaaaaa ccagcacggc aaatacccaa tcccaaagat
     4921 tcagattgct gatcaaagac ttctgaatca gcagctcgtg ttccggaagg gttttatagt
     4981 gttcagtcat gattcaagtc cttgccgagc cgttgcagac tctcgacgtg ttttggaaat
     5041 tccttctgca aatcccgttc gctgcgggcc gaagacatgg caaaacggat tttgccgtct
     5101 gaaaacaata cccacgcccg tttttcgcgc acataaaaca tcaataccgt acccaatacc
     5161 aacagcaccg agccgagata gaccaaaagc gcacccgggg aacgggtcat ctgcaaaccc
     5221 gacgaacgca cctcggaaaa cccatcaagt tgcagcagca taggcgcggg atattcggtc
     5281 aaacccgtgt acgcatccat actgtgcagc aggaaacgat tccgcgcttc atcctgctgc
     5341 cattcgggca agccgtaccg gcgtatggtt tcatccaaag cagcgttcat cacgccgtaa
     5401 agcatttcgt agaaatagcc ctgcatctta tcctgctgct ctttcgggat attggacgta
     5461 ataaattcgt ccaatcccaa atagcctttt tgtgcaaaga tgttcagcgt gttttccgca
     5521 gccagcatga attgttcgcg gatttcggca ggtgcgcctt tggttgcgtc ggcaaccaga
     5581 cgtttgcgcc cttccccatc tttcaaaaac tcacgcaatg ccataaaggt gtccgctttc
     5641 aactgcttgt ccaaggggat acgcagccag cggtattgct gctgcaagcc gctgcgcgtg
     5701 ccggtaatcc aaaaataatc ctgttcctgc aaaaccggca gcatatagtt tttatattcg
     5761 accgcctgcc ctgccgcatc acggatacgg taaacaatgg aagggccgat attggtgtat
     5821 tttttacctt cctgagtaac ggcgcggaca tcgttcagcg tggatttcag gcttttttcc
     5881 cgttccgcgc cctcgctcat gtcctccaca ttcatagaag taaactgatc gaactcaaga
     5941 cgatatttgt gtttgccaat ttccaacgga aactggtgta tggatgttgc cttcaacacg
     6001 acaggctcgc gcgaagcatc acccaaattc cacgccttga atgtcaaatc cgaaccgccg
     6061 tcggcaaaac tcgcctgata aatcgtgatg ccgtgcaagg tcaaaggatg gttcacgcgg
     6121 atggtgcgct cgagtttctc accggttgcc ttatccgtta cttcaatatc actggcaaaa
     6181 tcgcgcggca tacccgtatt gtaaaaatcg atatggaatt ttttcagttt gacttcaaaa
     6241 ggcaagtcct gaaccaatat cccgttgtcg gcattcagga aaaccacatc cgcactctgc
     6301 ccctcggaaa tattgacgtt gcccctaaat gagagattgg acgcacccaa aatactttcg
     6361 ggcttgaaat ccttggcata aaccgcctga ttgtccggaa caatccgacc ggtcagcata
     6421 cccagtttca acagcaggtt actgtctatc aacccgccca ggcaaatgac aatcaaagca
     6481 acatgggcaa agatatagcc ccatttgttc attgtgcctt ttttggcggc aatcagaacc
     6541 gacccgtctt cacggttaat ggtttttccc tgaaaacctt gtacttccag ataacgtttg
     6601 gcaacctcgg gcgcaatgtt tacatccaac agcgaagaat ggcgcatcgc cgccagagat
     6661 ttttctttaa ccttttcccg aaaagacttc atttcgcgcc agaacggcgg cacattgcga
     6721 atcaggcaca aactggtaga aaccaccaaa aacatcatga taacgacaaa ccatgccgaa
     6781 gcatagacgt catacagtcc cagaaaacca aaaatctgcg cccaaaacga tccgaatttg
     6841 accaaataat ccgtctgcgg ctggttttgc tgcaacaccg taccgataac cgatgcaata
     6901 cccagcagac tgagcaaagc gactgcaaag cgcatggagc tgaaaaaagc gaaccacgga
     6961 cgggaaagaa gtgggggaga tctacgggat ttactcattg tgtgtttatt ccgccatcag
     7021 gaatatggga aagcagaatt gggcaaacag aaaacaacgt cccgattcta ctgtcttgat
     7081 gctttttatt tcaagacaat gaagacagcc tgcatcgatt ccaacggttg cgattgaaaa
     7141 aacttatcgc agaattgcct gaagccgtct gaaaactttt cagacggcct ctaaaacaga
     7201 ctattgcgga attaacgcaa accttggata aagttggcga ccgctttcaa atcttcttca
     7261 gacatacggt ttgcaatatc ttccatgatg gtatttttac gctgaccgga cttgtaggca
     7321 ttcatctgtt caacaatata tgcctgatgc tgaccgccca aacgcggata agcctgaatt
     7381 tcgcttccgc ctcccggcat acccgcaccg ctcggaccgt ggcaggacat acacgccggc
     7441 acttttttat cgctcaaacc gccgcgatag attttcgcac ccaattcggg attttcctta
     7501 ggattggctt caccggattt gggctgctgt ttggcataga atgcggatac gttcaaaata
     7561 tcctgatcgc tcaaattcat taccaccggt ttcatcacag ctgccgaacc gtgggtgcgt
     7621 ttaccgtcgc ggatgccgat agtttgatga tagatgtaag cagtatgctg tgccgccaaa
     7681 cgcggataca tcgcaatgcc gctgttaccg tctgctgcat ggcaagccgc acaaaccgtt
     7741 gcggcaacct gtttgccttt ttccacgtct gctttgggag aggcggaaac cgcaccggca
     7801 gccaaaacaa aggccaataa agtcaatcgt ttcatggagt gctcctgatt acagcattgg
     7861 ataacgcaac aatgctcttt ttatattcaa atacgggatt tttgacccga ttaaaaccga
     7921 tgattctgta aacgtgttat tctatactaa atttacatta aattaccact gtgtttcaca
     7981 taaaaccaac cgcatatttt tgctgtcgga caaacggcgg cggaaaacaa ggatatgccc
     8041 atgaaccttt ttcaaaacgc caaattcttc acgacggtca accaccttaa agacctgccc
     8101 gacacccctc tcgaaattgc ctttgtcggc aggagcaatg ccggaaaatc cagtgccatc
     8161 aataccctga ccaaccatgt ccgtcttgcc tacgtttcaa aaacacccgg acggacgcag
     8221 catatcaact tcttcgagct gcagaacggc aattttatgg tcgatttgcc cggctacggt
     8281 tatgcccaag tccctgaagc agtacgcgca cattgggtca atctgctcgg cgactatctg
     8341 caacagcgca aacagcttat cgggctggtt ttgattatgg atgcccgcca tcctttaaaa
     8401 gaactcgaca tccgtatgct ggattttttc cacacgaccg gcagaccggt tcacatcctg
     8461 ctgtcaaaag ccgacaaatt atccaaaaac gaacagataa aaaccctgtc ccaagtcaaa
     8521 aaactgctca aaccttattc cgacaggcaa aacatcagcg tacagctgtt ttccagcctg
     8581 aaaaaacaag gtattgacga ggccaaccga actgtcggaa gctggtttga cgcagcagat
     8641 gccgccgctt cctctccaga ggaaaactga ccccaattat acggaaaccg tatttccccc
     8701 acttgaccga ccgcaaacat ttaaaaaatt gccactgcca aatctaaaat gccgtctgaa
     8761 agacttttca gacggcattt tgcggagtct ttaaaacaga gaatccaact gctgctgttt
     8821 ggaaccagta ttactcggaa gcaccggcgt ttcctgcata tcttggcgga cttcgtcatc
     8881 cgccgcctgc cgtccgcctt ctgccgcgcc cccgtcatct tcttttgccc gtcgggaagg
     8941 ttgcggcgca ataccgctgt tgtccagcgt caagcccgga tcggttacca tacgttcctt
     9001 catatagtat tcgccattgc tgctgaccac accttcaggc attttcatcc ccttgccctg
     9061 ctttcctttc aacgcaaaac gcatatagtc cacccaaacc ggcaccgcaa tcgtaccgcc
     9121 gtagccgaca cgccccatac tcttaggttt gtcgaagccg atatatacgg cagtaaccac
     9181 atcagggtta aaaccgacaa accacgcatc cttattgtca ttggtcgtac ccgttttacc
     9241 ggcaatatcc gttcttccca acgcagctgc cccccttgcc gtaccaacac ggaccacatc
     9301 ctgcataatc ttatacataa tataggcatt gcgcggatcg attgcctgag gcgcattttg
     9361 cccggccacc aaaggttgca tttgggcgcg caacctgccg tctctgtcat aaatcttatc
     9421 gattacgtgc gaagaaaccc tatatccgcc gttcgcaaat acgctatatg cctccgccac
     9481 tttcaacggc gttgtctcgc ccgtacctaa agccatagac aggcttgccg gcagctcgga
     9541 cgacctgaag ccgaaacgcc ggatatactg ttgcgcgtaa ccgacaccga tagacatcaa
     9601 aatacggatg gaaaccatat tcttggaagc cgtcagagcc tgtctcaaag taatgtagcc
     9661 ggaatatctg ccgtctgaat ttttaggtgt ccaaaccgaa ccgttcggcc ctttccccgg
     9721 cagggaaatc ggcgcatcgt taaccactgt ggacgcggtc atccccttag ataatgccgc
     9781 cgaatagaca aacggcttaa aggtcgaacc cggctgccgc attgcctgaa cggcacgatt
     9841 gaatgttttg ctgtgaaaat cataaccgcc gaccagcgcg cgcacagctc cggtttttgc
     9901 atccagcgaa accaaagccc cctgcagcaa cggctcttga accaccgccc aacgcccgcc
     9961 gttgtttttg acacggatga ccgcgcccct gcggatacgg tcctccccca ttttttcatt
    10021 attgaccgcg cgggccgcaa aacccaaggc gcgcctgtca agcgtaaccc gcctgccgcc
    10081 gggcagctgt atgacgacat ttttcttttt agtcacatcc aacacaacgg cgggaaccat
    10141 tttatcgacg gtatagagtc ccgacagata ctggctgaca gtctcctcga catcttcact
    10201 cttactcaaa tcgatatagt tttccgcacc gcggtagctg ctgccgcgat cgaaattccg
    10261 tagagccttg cgcaatgcct cggttgccac cttctgatga tcagcgcgga ccgtggtata
    10321 aaccttaaaa ccctgcgtat aggcatcttc accgtatttc tcatacagtt cctgacgcac
    10381 catttccgcc acatataaag cactctgatc gattttccga acaaaccgct cgtaatgcag
    10441 ttcctcattc aacgcctgat cgcgctgttg cacggtaatc atcttctcct cgagcatatt
    10501 gttcaaaata tacttctggc gcaacttggc acgttctgga ttaacaatcg gattataggc
    10561 agacggagcc ttgggcagtc ccgcaagcat ggcggcttcc gccaaagtca aatctcggac
    10621 attcttattg aaatagattt gcgcggcaga tgcaaaacca taggcgcgct gaccgaggta
    10681 aatctgattg aaatacaact cgaggatttt gtctttgctt aaagactgct cgattttata
    10741 ggcaagcaac acctcattga atttgcgtgt gaacgttttt tcactgctca aataaaaatt
    10801 tttcgccacc tgctgcgtaa tcgtactcgc acccgactgc acgctgccgg acacgacatt
    10861 gccgacggca gcgcgggcaa caccccaaac atccaccccc caatgccggt aaaagcgttt
    10921 atcctcggcg gcgataaccg cattccgcaa cacctttggg aaatcgccga tttttgtaaa
    10981 ttcgcgccgc tgctccccat acataccgat gacttcccca tccgccgaat aaatagtcaa
    11041 cggcatttta ggctggtaat gctgcaaaga atccaaagac ggtagtttcg gatacgttac
    11101 caaaatagca atggcaacca aacccactcc aaatacacaa aacccaaaaa ccaaaccaaa
    11161 acaagtcgtt aaaatctttt taatcatagc tgaataataa tttaccatta ttggtattaa
    11221 ataaagtaaa atagcaaccg atttctacaa agcacggttt caatgtgcaa agaacaagga
    11281 atccattacg gataccgaaa cggttactca ctgtacaaat aaagcaggaa ctttcatcat
    11341 gcgcttgttt aaaagcttga aaaaccctaa aaaaacagat gccaagctcc ctaaaaaatc
    11401 ttcgggactc aataaccgcg cggcaatcgg catcgatatc gaccagcatt ccatcaaaat
    11461 ggtccaattg tcaggacgta gtttaaacca aattcaattg gaaaaatacg tcattgccaa
    11521 attaccaaag aatatcattc aaggcaataa agtccaaaat tacgatcaac ttgttacata
    11581 tttgcaacaa gcctatgcca aactgggtac ttcgtgcaaa aacatcatcg cgtccgtccc
    11641 gcaaaatctg gcaaccatcg aacaattgac ctacacagac aaagatgcag aattagacct
    11701 gcaggggttc gtggagtcct ccatctccga agtcagctcg atatcgctcg aagaagccaa
    11761 ttacgactat caggtcttgt cccaatcggc cgccggcgaa gctgtgttgg ccgtcgcatc
    11821 gagaaaggat gaaatcgaac ccctgattga cgcattcaac gccgccggta tgaaattatc
    11881 cgcgcttgat gtggacattt tcggacaata caacgcctac gcgctatgga taaaccattt
    11941 cgcccccgag cttgcagccg aaaaagtcgc cattttcggc gtatatgccg cacagaccta
    12001 cgccttggtc atccaagacg gaaaaatcct atacaaacag gaaacctccg tcagcgaaga
    12061 acagctcaac caactcatcc agcgcaccta tcaggtaaca gaagaaaaag cggaagaaat
    12121 catcaactcc ccgcaaaaac cttccgatta ccaagaaagc gtggcaaact atttcaacca
    12181 gcagattacc caagaaatac aaagggtctt gcagttttat tacaccacgc agaccgcaga
    12241 cgatatgacc gacatcaagc atatcctgct gaccggggaa gcggcgcgcc aggaaggcat
    12301 cgcccaaacc gtcgcctcac aaaccaatgc agatgtacaa tgcgtccatc ccgcgcgtta
    12361 ttttgcggac aacctcaaaa cagacaaaca acaattcgaa cttgatgcgc cgacactgac
    12421 cagggcgttc ggtttggcgg tacggggatt ataattatga acaatttaat caaaatcaac
    12481 ctcctcccct acagggaaga gatgaacaag cgcaaacagc agcagtttaa aacgctgatg
    12541 tacggtgccg tgctgacggg cgttgccgcc gttgcggcaa cctacctgtt tatcgacaat
    12601 atgatcaata accagtcgga aagaaacacg ctgctggaaa cctccatcgc acacttggat
    12661 accgagctgt cggaaataca aaagctcaaa caggaaaaag atgccttcct gattaagaaa
    12721 aacaaaatcg aggagctcca gctcaaacgc ctccaagccg caaaaatcct cgacagcctg
    12781 aatgaggccg tccccggaag cacctacctg acctcgctgg atgccgttac cgccgactct
    12841 tatcggctca gcggcaggac atccagcgac aaccgcgttg ccgccatgat gagggcgatg
    12901 cccaataccg gcatattcaa gcaacccgaa ttgttaagca tcaagaaaaa caattcgcat
    12961 caagaattta cccttcaggc aacattacaa cccatcgtaa aggcggccga atccaaagag
    13021 aatccggctt cgggaaacgc acaggaggca aactgaatgg cttctaaatc atctaaaacc
    13081 aacttggatc tcaacaacct tcacctgctc aaccttcctg ccaggctttt tatcgccctg
    13141 ctggccgttg ccgccgtgct ggggctcggt tatgccggat tgttcaaaag ccagatggaa
    13201 tcccttgagg aatacgaagc aaaagaaacc gaactgaaaa acacctacaa acagaaaagt
    13261 atcgacgcgg ccagcctgaa caacctgagg gacgaacttg cctcaatccg ctctgccttc
    13321 gatatcatgt tgaaacagct gccgacagat gcagaaattc ccaacctggt tcaagagctt
    13381 catcaggcag gttcgagcaa cggtctgcgc ttggacagcg ttatgcccca acctcccgta
    13441 gatgacggcc ccatcaaaag attaccctat tccatttcca ttaccggaaa ttacgaacag
    13501 atcagccaat ttacccgcga tgtcggcagc ctctcccgaa tcattaccct tgagtcgctg
    13561 aaaatcgccc aatctccgga aaacggcggc aatcctgacg gcaagagcag catcctgaac
    13621 ctcagcgcca ttgccaccac ctaccaagca aaatccgtag aagagcttgc cgcagaagcg
    13681 gcacaaaatg ccgagcaaaa ataacttacg ttagggaaac catgaaacac tatgccttac
    13741 tcatcagctt tctggctctc tccgcgtgtt cccaaggttc tgaggaccta aacgaatgga
    13801 tggcacaaac gcgacgcgaa gccaaagcag aaatcatacc tttccaagca cctaccctgc
    13861 cggttgcgcc ggtatacagc ccgccgcagc ttacagggcc gaacgcattc gacttccgcc
    13921 gcatggaaac cgacaaaaaa ggggaaaatg cccccgacac caagcgtatt aaagaaacgc
    13981 tggaaaaatt cagtttggaa aatatgcgtt atgtcggcat tttgaagtcc ggacagaaag
    14041 tctccggctt catcgaggct gaaggttatg tctacactgt cggtgtcggc aactatttgg
    14101 gacaaaacta cggtagaatc gaaagcatta ccgacgacag catcgtcctg aacgagctaa
    14161 tagaagacag cacgggcaac tgggtttccc gtaaagcaga actgctgttg aattcttccg
    14221 acaaaaacac cgaacaagcg gcagcacctg ccgcagaaca aaattaagaa gaggattact
    14281 ccattatgaa taccaaactg acaaaaatca tttccggtct ctttgtcgca accgccgcct
    14341 ttcagacagc atcggcagga aacattacag acatcaaagt ttcctccctg cccaacaaac
    14401 agaaaatcgt caaagtcagc tttgacaaag agattgtcaa cccgaccggc ttcgtaacct
    14461 cctcaccggc ccgcatcgcc ttggactttg aacaaaccgg catttccatg gatcaacagg
    14521 tactcgaata tgccgatcct ctgttgagca aaatcagtgc cgcacaaaac agcagccgtg
    14581 cgcgtctggt tctgaatctg aacaaaccgg gccaatacaa taccgaagta cgcgggaaca
    14641 aagtttggat attcattaac gaatcggacg ataccgtgtc cgcccccgca cgccccgccg
    14701 taaaagccgc gcctgccgca ccggcaaaac aacaggctgc cgcaccgtct accaagtccg
    14761 cagtatccgt atccgaaccc tttaccccgg caaaacaaca ggctgccgca ccgtttaccg
    14821 agtccgtagt atccgtatcc gcaccgttca gcccggcaaa acaacaggcg gcggcatcag
    14881 caaaacaaca ggcggcagca ccagcaaaac aaaccaatat cgatttccgc aaagacggca
    14941 aaaatgccgg cattatcgaa ttggctgcat tgggctttgc cgggcagccc gacatcagcc
    15001 aacagcacga ccacatcatc gttacgctga aaaaccatac cctgccgacc acgctccaac
    15061 gcagtttgga tgtggcagac tttaaaacac cggttcaaaa ggttacgctg aaacgcctca
    15121 ataacgacac ccagctgatt atcacaacag ccggcaactg ggaactcgtc aacaaatccg
    15181 ccgcgcccgg atactttacc ttccaagtcc tgccgaaaaa acaaaacctc gagtcaggcg
    15241 gcgtgaacaa tgcgcccaaa accttcacag gccggaaaat ctcccttgac ttccaagatg
    15301 tcgaaatccg caccatcctg cagattttgg caaaagaatc cggaatgaac attgttgcca
    15361 gcgactccgt caacggcaaa atgaccctct ccctcaagga tgtgccttgg gatcaggctt
    15421 tggatttggt tatgcaggcg cgcaacctcg atatgcgcca gcaagggaat atcgtcaaca
    15481 tcgcgccccg cgacgagctg cttgccaaag acaaagccct cttacaggca gaaaaagaca
    15541 ttgccgattt gggtgcgctg tattcccaaa acttccagtt gaaatacaaa aatgtggaag
    15601 aattccgcag catcctgcgt ttggacaatg ccgacacgac cggaaaccgc aacacgctta
    15661 tcagcggcag gggcagcgtg ctgatcgatc ccgccaccaa caccctgatt gttaccgaca
    15721 cccgcagcgt catcgaaaaa ttccgcaaac tgattgacga attggacgta cccgcgcaac
    15781 aagtgatgat tgaggcgcgt atcgtcgaag cggcagacgg cttctcgcgc gatttgggcg
    15841 ttaaattcgg cgcgacaggc aagaaaaagc tgaaaaatga tacaagcgca ttcggctggg
    15901 gggtaaactc cggcttcggc ggcgacgata aatggggggc cgaaaccaaa atcaacctgc
    15961 cgattaccgc tgccgcaaac agcatttcgc tggtgcgcgc gatttcctcc ggtgccttga
    16021 atttggaatt gtccgcatcc gaatcgcttt caaaaaccaa aacgcttgcc aatccgcgcg
    16081 tgctgaccca aaaccgcaaa gaggccaaaa tcgaatccgg ttacgaaatt cctttcaccg
    16141 taacctcaat cgcgaacggc ggcagcagca cgaacacgga actcaaaaaa gccgtcttgg
    16201 ggctgaccgt tacgccgaac atcacgcccg acggccaaat cattatgacc gtcaaaatca
    16261 acaaggactc gcctgcgcaa tgtgcctccg gtaatcagac gatcctgtgt atttcgacca
    16321 aaaacctgaa tacgcaggct atggttgaaa acggcggcac attgattgtc ggcggtattt
    16381 atgaagaaga caacggcaat acgctgacca aagtccccct gttgggcgac atccccgtta
    16441 tcggcaacct ctttaaaaca cgcgggaaaa aaaccgaccg ccgcgaactg ctgattttca
    16501 ttaccccgag gattatgggt acggccggca acagcctgcg ctattgatgc gtcaaaataa
    16561 gggcatatgt tttacggcat atgccctttc tttatgcttt ttgccgcgac cgaaatgccg
    16621 tcattcccgc gcaggcagaa atccaacttg tccggttccg gttgtttttc gtctcgtaac
    16681 ttttgagccg tcattcccgc gcaggcagaa atccagtccg ttcagtttcg gtcatttccg
    16741 ataaattcct gttgcttttc atttctggat tcccacttt
//
LOCUS       DAWFAN010000029        16458 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWFAN010000029 DAWFAN010000000
VERSION     DAWFAN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.029"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            210..1274
                     /locus_tag="WGO65_000675"
     CDS             210..1274
                     /locus_tag="WGO65_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ0327001.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKEGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1415..1738
                     /locus_tag="WGO65_000676"
     CDS             1415..1738
                     /locus_tag="WGO65_000676"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ0327002.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1810..2133
                     /locus_tag="WGO65_000677"
     CDS             1810..2133
                     /locus_tag="WGO65_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ0327003.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2180..2383
                     /gene="tatA"
                     /locus_tag="WGO65_000678"
     CDS             2180..2383
                     /gene="tatA"
                     /locus_tag="WGO65_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ0327004.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2387..3073
                     /gene="tatB"
                     /locus_tag="WGO65_000679"
     CDS             2387..3073
                     /gene="tatB"
                     /locus_tag="WGO65_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ0327005.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKEIGTDMEGNLHDISNGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANPASDGISDVMPSERSYTSAETLGDDRQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3087..3857
                     /gene="tatC"
                     /locus_tag="WGO65_000680"
     CDS             3087..3857
                     /gene="tatC"
                     /locus_tag="WGO65_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ0327006.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAVITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3910..4515
                     /gene="maf"
                     /locus_tag="WGO65_000681"
     CDS             3910..4515
                     /gene="maf"
                     /locus_tag="WGO65_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ0327007.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAATFLNLL
                     SGTGHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIGGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4531..4884
                     /locus_tag="WGO65_000682"
     CDS             4531..4884
                     /locus_tag="WGO65_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ0327008.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4874..6680
                     /locus_tag="WGO65_000683"
                     /pseudo
     CDS             4874..6680
                     /locus_tag="WGO65_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
     gene            6870..7547
                     /locus_tag="WGO65_000684"
     CDS             6870..7547
                     /locus_tag="WGO65_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0327009.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7560..8966
                     /locus_tag="WGO65_000685"
     CDS             7560..8966
                     /locus_tag="WGO65_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0327010.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9084..11510
                     /locus_tag="WGO65_000686"
     CDS             9084..11510
                     /locus_tag="WGO65_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ0327011.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGSADLIIAVTPPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFGSHPLYGDILTACCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HLKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLSPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTARDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            11555..11800
                     /gene="rpsP"
                     /locus_tag="WGO65_000687"
     CDS             11555..11800
                     /gene="rpsP"
                     /locus_tag="WGO65_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ0327012.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11816..12325
                     /gene="rimM"
                     /locus_tag="WGO65_000688"
     CDS             11816..12325
                     /gene="rimM"
                     /locus_tag="WGO65_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ0327013.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLHGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12325..13074
                     /gene="trmD"
                     /locus_tag="WGO65_000689"
     CDS             12325..13074
                     /gene="trmD"
                     /locus_tag="WGO65_000689"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ0327014.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13090..13455
                     /gene="rplS"
                     /locus_tag="WGO65_000690"
     CDS             13090..13455
                     /gene="rplS"
                     /locus_tag="WGO65_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ0327015.1"
                     /translation="MNLIQQLEQEEIARLNKDIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13774..14499
                     /locus_tag="WGO65_000691"
     CDS             13774..14499
                     /locus_tag="WGO65_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ0327016.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDTRTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14535..15410
                     /locus_tag="WGO65_000692"
     CDS             14535..15410
                     /locus_tag="WGO65_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ0327017.1"
                     /translation="MNPYDLLITPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15487..16413
                     /locus_tag="WGO65_000693"
     CDS             15487..16413
                     /locus_tag="WGO65_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEZ0327018.1"
                     /translation="MKHPKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALI
                     KADPEGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHI
                     EFIAPQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETSVNVSG
                     KLYSDALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4269 a         5287 c         3618 g         3284 t
ORIGIN      
        1 gccatctgaa gccgattcgg ttttcagacg gcatcattat ttgacctgac gcaaacagaa
       61 agccttccgc gtgcttaaac acatctcttg tagtaaaata tctaaacaga ttcaactgga
      121 aacggtcagg cttcttcacg ccattcccat ttcaaccgcc gcctgccaga ttcagacggt
      181 attcctactt gaaaacaagg agcgttacaa tgaaagcagc acgtttttac gacaaaggcg
      241 acatccgcat cgaagacatc cccgaaccga ccgtcgcccc cggcaccgtc ggcatcaatg
      301 ttgcctggtg cggcatctgc ggtaccgacc tgcacgaatt catggaaggc ccgattttca
      361 ttccgccttg cggtcatccg cacccgattt ccggcgagtc cgcacccgtc acgatgggac
      421 acgagttctc cggcgtggtc tatgccgtcg gcgaaggcgt ggacgacatc aaagtcggtc
      481 aacacgtcgt tgtcgagcct tacatcgtac acgacgacgt accgaccggc ccgggcagca
      541 actaccacct ctctaaagac atgaacttca tcggcttggg cggctgcgga ggtggcttgt
      601 ctgaaaaaat cgctgtcaaa cgccgttggg tgcatccgat ttccgacaaa atcccgttgg
      661 atcaagccgc tttgatcgaa ccgctgtctg tcggacacca cgcctatgta cgcagcggcg
      721 cgaaagaagg cgatgtcgca ttggtcggcg gtgcaggccc gatcggtttg ctgttggctg
      781 ccgtgctgaa agccaaaggc atcaaagtca tcatcaccga gttgagtaaa gcacgcaaag
      841 acaaagcgcg cgaatccggc gttgccgact acatcctcga cccgtccgaa gtcgatgttg
      901 tcgaagaagt gaaaaaactg accaacggcg aaggcgtgga cgtggcattc gaatgtacca
      961 gcgtgaacaa agtgttggat actttggtcg aagcctgcaa acctgccgcc aatttggtta
     1021 tcgtatccat ctggagccac cctgcaaccg tcaacgtcca cagcgtcgtg atgaaagagc
     1081 tggatgtgcg cggcacgatt gcctactgta acgaccacgc cgaaaccatc aaactggtcg
     1141 aagaaggcaa aatcaacctc gaacccttca tcacccagcg catcaagctg gacgaactgg
     1201 tttccgaagg cttcgagcgt ctgatccaca acaacgaatc cgccgttaaa attattgtga
     1261 gtccaaacct gtaagcaaat tacatttgca acagcacaaa tgccgtctga aacgctcaga
     1321 cggcattttc caacgaagcg caacaggcgt attgcccgag agcagccgtt tagcctatca
     1381 ttccgaacaa acagaaacaa acagaaggaa aatcatggga gattccgtac tatccgccat
     1441 ccaacaaacc atcacccagc gaaaatctgc caatccgtcc gaatcttacg tcgcacagct
     1501 cttgcataag ggcgaagaca aaatcctaaa aaaagtgatt gaagaggcgg gcgaagtgtt
     1561 gatggcatcc aaagacaaaa acccgtccca cctggtttac gaagttgccg acttatggtt
     1621 tcacaccatg attcttctga cacaccacga cctgaaggcg gaagacgtat tggacgaact
     1681 tgcgcgccgc caaggtttgt cgggcttggc cgaaaaagcc gctcgcacag aatcttgaat
     1741 ttatattaaa atccgcactt tcccacattc aatccgtctg accgcttttc agacggcatc
     1801 ggagccgtta tggacaactg tattttctgc aaaatcgccg ccaaagacat tccggcgcaa
     1861 accgtctacg aagacggcga aatggtttgt ttcaaagaca tcaaccccgc tgctccggtt
     1921 catctgctgc tgattcccaa agtccatttc gattcgttgg cacacgccgc gcccgaacat
     1981 cagccccttt tgggaaaaat gatgctgaaa gttcccgaaa tcgccaaagc ggcaggactg
     2041 gcagacggct tcaaaaccct gatcaacacc ggaaaaggcg gcggacaaga ggtcttccac
     2101 ctgcatatac acatcatggg cacacccgta taaaccgtta tttcacaatc aacccctaat
     2161 acttacttaa ggatacatca tgggcagttt ttctctgacg cactggatta tcgtactgat
     2221 tatcgtcgtt ttgatattcg gcaccaaaaa actgcgcaac gtcggcaaag acctcggcgg
     2281 tgcggttcat gacttcaaac aggggctgaa cgaaggcaca gacggcaaag aagcccaaaa
     2341 agacgatgta atcgaacaca aaaaagacga agacaaagcg taatttatgt ttgatttcgg
     2401 tttgggcgag ctggtttttg tcggcattat cgccctgatt gtcctcggcc ccgaacgcct
     2461 gcccgaggcc gcccgcaccg ccggacggct catcggcagg ctgcaacgct ttgtcggcag
     2521 cgtcaaacag gaatttgaca ctcaaatcga actggaagaa ctgaggaagg ccaagcagga
     2581 attcgaagct gccgccgctc aggttcgaga cagcctcaaa gaaatcggta cggatatgga
     2641 gggtaatctg cacgacattt ccaatggtct gaagccttgg gaaaaactgc ccgaacagcg
     2701 cacgcctgct gatttcggtg tcgatgaaaa cggcaatccg cttcccgatg cggcaaaccc
     2761 cgcatcagac ggcatttccg acgttatgcc gtccgaacgt tcctacactt ccgccgaaac
     2821 ccttggagac gacaggcaaa ccggcagtac agccgaaccc gcggaaaccg accaagaccg
     2881 cgcatggcgg gaatacctga ctgcttctgc cgccgcaccc gtcgtacaga ccgtcgaagt
     2941 cagctatatc gataccgctg ttgaaacccc tgttccgcat accacttcgc tgcgtaaaca
     3001 ggcaataagc cgcaaacgcg atttgcgtcc caaatcccgc gccaaaccta aattgcgcgt
     3061 ccgtaaatca taaagagggc aatccggtgt ccgaaacaca aaacgaacaa cccgtccaac
     3121 cgcttgtcga gcatctcatc gagctgcgcc gccgcctgat gtggacggtt gtcggcatct
     3181 tagtctgctt tttcggccta atgccgtttg cccaacaact ctatactttt atcgccgacc
     3241 cgctgatggc aaacctgccc aaagacacca gcatgattgc caccgatgtc atcgcaccgt
     3301 ttttcgtgcc ggtcaaagtt accctgatgg cggcattttt aatttcgctg ccgcatacgc
     3361 tctaccaaat ctgggcattt gtcgcgcccg cactctacca aaacgaaaaa cgcctgatta
     3421 cgccgctcgt cctctccagc gtcagcctgt ttttcatcgg catggcattt gcctactttt
     3481 tggttttccc cgtcattttc aaattccttg ccagcgttac ccctgtcggt gtcaatatgg
     3541 cgacagacat cgacaaatac ctctccttca tcttggggat gtttgtcgca ttcggtacaa
     3601 cgtttgaagt ccccattgtc gttatcctgt taaccaaaat tggtgtggta acaaccgaac
     3661 agctcaaacg cgcccgcccc tatgtgattg tcggcgcgtt tgtcattgcc gcagtcatca
     3721 caccgcccga tgtgatttca caaaccctgc ttgccattcc gctgattctc ttatacgaag
     3781 caggtatttg gttcggacgc tttttcacgc cacgttcaga acaggatggc gacatacagc
     3841 cgcctgcaac aacctgacac tatgccgtcc gaacctccgc ctcataccgc aacagattaa
     3901 ggaatacctt tgaataccct ctatttaggt tcaaacagcc cgcgccgaat ggaaatcctg
     3961 acacagttgg gctatcaggt cgtcaagctg cctgccgaca tcaatgaaac ggtcagacag
     4021 aacgaagacc ctgcccgtta cgttcaaagg atggcagaag aaaaaaaccg aaccgccctg
     4081 accctctttt gcgaaaccaa cggcacaatg cccgatttcc ccctgattac cgccgacacc
     4141 tgcgtcgttt cagacggcat catattgggc aaaccccgct cccaagctga agcggcaacc
     4201 tttctaaacc tgctgtcggg aaccgggcat accgtcctga ctgctgtctg cattcattat
     4261 cgcggcaaaa cgtcaagccg cgtccaaacc aaccgcgtcg ttttcaagcc cctgagttcg
     4321 gaagaaattt ccgcctatgt gcaaagcggc gaaccgatgg acaaagccgg tgcctacgcc
     4381 gtacaaggca taggcggcat ctttatccaa tctatcggag gcagcttcag cggcattatg
     4441 gggctgcccg tttatgaaac cgtttcgatg ttgcaggatt tgggataccg taccccacct
     4501 ttcatccgag cctaacccgt aaagaccgcc gtgaacagac aaaccgctta cctccttacc
     4561 tctttcagcc tgatcgcact gataatcctg tccctttcct gggaactgtg gattgcaccg
     4621 ttgcgcccgg gcggctcgtg gctcgcgctc aaagccctcc ccctctgtct gccgctttca
     4681 ggcatcttga aaaagaaaat ctatacttac caatacagct ccatgctggt tctgatttac
     4741 tttgccgaag ccgtcatgcg cctgttcgac gcctatcccg cagaacagat ttgcgccgcg
     4801 ctttccgcag tattcagcat catcttcttc atatcctgcc tgtcgttcgt caaacaatac
     4861 aaggaaacaa acaatgcccg ctgaaacgac cgtatccggc gcgcaccccg ccgccaaact
     4921 gccgatttac atcctgccct gcttcctttg gataggcatc gtccccttta ccttcgcgct
     4981 caggcagcaa ccgtcgcccg acttttacca cgatgctgca gccgcagccg gcctgattgt
     5041 cctgttgttc ctcacggcag gaaaaaaact gtttgatgtc aaaatccccg ccatcagctt
     5101 ccttctgttt gcaatggcgg cgttttggta tcttcaggca cgcctgatga acctgattta
     5161 ccccggtatg aacgacatcg tctcttggat tttcatcttg ctcgccgtca gcgcgtggac
     5221 ctgccggagc ttggtcgcac acttcggaca agaacgcatc gttaccctgc ttgcctggtc
     5281 gctgcttatc ggctccctgc ttcaatcctg catcgtcgtc atccagtttg ccggctggcc
     5341 ccatgcttca aaacattatt gtttacagcg ggcaaggcgt aatcggacac atcggacagc
     5401 gcaacaacct cggacactac ctgatgtggg gcatactcgc cgccgcctac ctcaacggac
     5461 aacgaaaaat cccgcccgcc ctcggcgcaa tctgcctgat tatgcagacc gccgttttag
     5521 gtttggtcaa ttcgcgcacc atcttgacct acatagccgc catcgccctc atccttccct
     5581 tctggtattt ccgttcggac aaatccaaca ggcggacgat gctcggcata gccgcagccg
     5641 tattccttac cgcgctgttc caattttcca tgaacaccat tctggaaacc tttactggca
     5701 tccgctacga aactgccgtc gaacgcgtcg ccaacggcgg tttcacagac ttgccgcgcc
     5761 aaatcgaatg gaataaagcc cttgccgcct tccagtccgc cccgatattc gggcacggct
     5821 ggaacagttt tgcccaacaa accttcctca tcaatgccga acagcacaac atatacgaca
     5881 acctcctcag caacttgttc acccattccc acaacatcgt cctccaactc cttgcagaga
     5941 tgggaatcag cggcacgctt ctggttgccg caaccctgct gacgggcatt gccgggctgc
     6001 ttaaacgccc cctgaccccc gcatcgcttt tcctgatctg cacacttgcc gtcagtatgt
     6061 gccacagtat gctcgaatat cctttgtggt atgtctattt cctcatcccc ttcggactga
     6121 tgctctttct gtcccccgca gaggcttcag acggcatcgc cttcaaaaaa gccgccaatc
     6181 tcggcatact aaccgcctcc gccgccatat tcgcaggatt gctgcacttg gactggacat
     6241 acacccggat ggttaacgcc ttttcccccg ccactgacga cagtgccaaa accctcaacc
     6301 ggaaaatcaa cgagttgcgc tatatttccg caaacagtcc gatgctgtcc ttttatgccg
     6361 acttctccct cgtaaacttc gccctgccgg aataccccga aacccagact tgggcggaag
     6421 aagcaaccct caaatcacta aaataccgcc cccactccgc cacctaccgc atcgccctct
     6481 acctgatgcg gcaaggcaaa gttgcagaag caaaacaatg gatgcgggcg acacagtcct
     6541 attaccccta cctgatgccc cgatacgccg acgaaatccg caaactgccc gtatgggcgc
     6601 cgctgctacc cgaactgctc aaagactgca aagccttcgc cgccgcgccc ggtcatccgg
     6661 aagcaaaacc ctgcaaatga cccgcgccgg cggatgcgga taccgcccga aatgtaaagc
     6721 tccatgcaag acattgcaaa aaacagcaaa ccggtaggga aatacgctat caaaaaacat
     6781 tgcagccgtg ttaagataaa ccgtcaaaca atcttttcac gccccgcccg aaacagggtc
     6841 ggggcatacc cttacgaaaa ggaaacacca tgagccgcgt attactcgta gatgacgatg
     6901 ccctgctaac cgaactgctg accgaatacc tgagcgccga aggtctgaac gtccgcagcg
     6961 ttcccgacgg ggaagcaggc gtacaggaaa tcctgagcgg gcagtacgat gtagtcgtat
     7021 tggattccat gatgcccaaa atgaacggct tggatgtctt gaaaaacgta cgcgcccgaa
     7081 gcaccgtccc catcatcatg ctgaccgcca aaggcgacga catcgaccga atcatcggct
     7141 tggaaatggg cgcggacgac tatgtcccca aaccctgcac accacgcgaa ctcttggcac
     7201 gcatcaatgc catcctgcgc cgcgcacaac acagcggcga acagaacaac gcacccaaca
     7261 gcatctccgt cagcgatgtc gtcctgtacc ccgccaaacg ccaggcatcc gtcaaagaca
     7321 tgccgctcga actgaccagc accgaattca acctgctcga agtcctgatg cgccatgccg
     7381 gacaggtagt cagcaaagaa accctgtccg tcgaagcact cgaccgcaag ctggcaaaat
     7441 tcgaccgcag tatcgacgta cacatctcca gcatccgcca caagttgggc gatgcctctc
     7501 tgattcaaac cgtacgcggc ttgggctacc tgtttgtcaa aaactgaagc aacgggcaaa
     7561 tgaaactgtt ccaacgcatc ttcgccacat tttgcgcggt tatcgtctgt gcaatctttg
     7621 tggcgagttt ttctttttgg ctggtgcaga acacccttgc cgaaaaccag ttcaaccaac
     7681 gccgcaccat cgaaaccact ttgatgggca gcatcatttc cgcattccgg gcacgcgggg
     7741 acgcgggcgc gcgcgaaatc ctgacggaat ggaaagacag ccccgtctca tcgggcgtgt
     7801 acgttataca gggcgacgag aaaaaagata tcctgaaccg gtatatcgac agctatacca
     7861 tcgaacgcgc ccggcttttc gccgccggac acccgcattc caacctcgtc catatcgaat
     7921 acgaccgctt cggcgaagaa tacctgttct tcaccaaaga ctgggacaaa ctccaagccc
     7981 gccgcctgcc cagccccctg ttgatccccg gcctgccgct cgccccgatt tggcacgaac
     8041 tcatcatatt gtccttcatc atcatcgtcg gactgctgat ggcatatatc ctcgccggca
     8101 acattgccaa acccatcaga atcttaggca acggcatgga cagggtggca aacggagaac
     8161 ttgaaacccg tatctcccaa caggtcgacg accgcgacga cgaattgtcc catcttgcca
     8221 tccaattcga caaaatggtg gaaaaactcg aaaaactcgt tgccaaagaa cgccacctgc
     8281 tccatcacgt ctcccatgaa atgcgttctc cccttgcgcg catgcaggca attgtcggac
     8341 tgattcaggc gcagccccaa aaacaggagc aatatctcaa acggctggaa ggcgaactga
     8401 cccgcatgga tacgctggcc ggggaactgt taaccctgtc ccgtctcgaa acttccaata
     8461 tggctttgga aaaagaaagc ctgaaactcc tgcccttcct gggcaacctg gtagaagaca
     8521 atcaaagcat tgcccagaaa aacggacaaa cggttaccct gtctgccgac ggaaaaatcc
     8581 ccgaaaatac aaccatcctt gccaacgaaa gctacctgta ccgcgccttc gacaacgtca
     8641 tccgcaacgc cgtcaactac agtcccgaag gcagcaccat cctgatcaac atcggacaag
     8701 accacaaaca ctggataatc gacgttaccg acaacggccc cggcgtggac gaaatgcagc
     8761 tcccgcacat cttcaccgct ttctaccgtg cagactccag tgcgaacaaa cccggaacag
     8821 gactggggct tgcattgacc caacatatta ttgaacagca ctgcggaaaa atcatcgccg
     8881 aaaacatcaa accgaacggt ctgcggatgc gctttatcct gcccaagaaa aaaaccggtt
     8941 ccaaaacaga aaaaagtgcg aactgaccat aatgccgtct gaaccggctt cagacggcat
     9001 tgcacaaaca gttaccccaa aacaaacaca cagcgggtgc atctccaaca atcacgcaca
     9061 taccgtccgc aaaggaatat atcatgtcgg cacaaaccga tccgggctac tttttcatgc
     9121 ccaaccacat tatcctgata ggcgcgagcg aacgtccgta cagcctgggc gaacgcgtac
     9181 tcagcaacct gctgagtacg ccctttcaag gaaaaatcac ccccgtaaac ccgcgccacc
     9241 acaccatagc cgggcttccc gcctacacca gcctcaacaa aatccccggc agtgcagacc
     9301 tgattattgc cgttaccccg cccgacagtt acgacaccct cttcaaaacc tgccgcaaaa
     9361 agcagctccg acacatcatc ctcatacagg actgggacag cctgtctgcc gcagaactgc
     9421 acaccgccga aactgccatc cgcaaacacc acggcaacgg actcaacatc accgcctgca
     9481 ccaccgcagg catccaactg ccctcactcg gacttaacat cagtacccaa gacggatacg
     9541 ccgcaggcca taccgccata ctgaccggca atgccgccgt cagccgccaa atcgacaaca
     9601 tcctgaacaa actccgtcaa ggcacatccc gccacatcag cctgcatccc ggcatcagcc
     9661 ccatcacatc cgccgattgg ctcaaccgct tcggacacag cctgcacacc aaaaccgccg
     9721 tcctacacca caaccctgaa gaggatcagc gcaaactgtt cagcgcaatc cgccaattta
     9781 cccgccatac gccgctgatt ctccacatca cctgccgcac gacagaaacc gaccgtgccg
     9841 tactgcactg cctcgcccga cactgcaact tcctcatcag tttcaacgcc gacgacctcg
     9901 aagccgcact gcgcgcccaa ctgtccgacc ttccacccct gtcccgactc gacatcctgt
     9961 ccgacacgcc tgccgaatgg ctgaacgcgc acgcgccaaa aaccctcgcc ctccactttc
    10021 ccaaccttcc ccaccacatc cgcaacggac acctgaccgg cacacccaca ccctcaatct
    10081 gccacgacat cgcctcacgt cagcttgccc gccccgacac ccaagccgtc ctgaccatcc
    10141 tcagcccctc cggacacgag gattacaaaa aaacagcacg cgcccttatc cgcctgtccg
    10201 aacagaccgc caaacccctg ctcgtcagca gccccttttc agacggcata acacatttcg
    10261 acacccccac tcaggcaatc cgcacccttt cctaccgcaa caccgccgcc gccctgaaac
    10321 aggcacagct cgacattgca ccgccgcagc catgccgtct gaaaacaccg caaccccaaa
    10381 acatcaaaaa agcccttgca gcggcaaatc cctccctgct cgccgaagcc ctgcacctcc
    10441 ccccctaccg gcacactacc cataacgccg tccaattcca attcggcagc caccccctct
    10501 atggcgacat cctgaccgca tgctgcaacg gacaaaccac tgccgtactc ccgccgttta
    10561 ccacgctcga cagccgccac cttgcccgct ttgccgaact cgacggcaca caaaccctcg
    10621 accagttcct gcacacactg accgtcattc ccgaataccg ccaacacatt ctcggcatca
    10681 ccctcaacct caacggcgga caatacagca gcgatttcat cttaaagaca cccgaaacac
    10741 acgacacgcc caaacgcaag aacacaggca aagccgccca aaccctcgaa catgccgccg
    10801 caaaaatgca gagtgccgcc gcatacctga aacacaaaaa cccgacagcc gccgaatttc
    10861 tccgccacac aagcgaagcc gccgcagaac tgctcggcag caaaaccgaa accggagcag
    10921 ccgtacccaa cgtacttgcc ccctatcccg cagcacacct caaaacactg tccctaaaaa
    10981 acaacacgac cgttaccatt accccccttt tgcccgaaga cgcagaagcc aaacagcagt
    11041 tcgtccgcag cctcagcccc gaagcacggt acacacgttt catgacccac accaacgaac
    11101 tgcccgcagc cacgttggca cgcctgtgca accccgacta ccactgtgaa gccgcatgga
    11161 cggcaaggga tgccgacagc aacatcgtcg ccgtcgtccg ccacagccgc ctgaaccgca
    11221 acgaatgcga attcggcatc acactggcgg aacatatgcg cggcagcggg ctggcacaga
    11281 aaatgatgga actcatcatc caaaccgccg cacagcaagg ctaccggact atgagtgccg
    11341 acattctcaa aaccaatact cccatgatca aacttgctga aaaatcagga tttaccctca
    11401 aggaatcgga caccgaaaaa aacctgtacc gcgcatatct gaaccttgcg gcagacaaaa
    11461 caacagaaaa aacaaataaa aacttgcaca ccgaccacaa aataacctaa aatcggcagt
    11521 ttccatatat ccgattttct caaaaggact caaaatggta gttatccgtt tggcacgcgg
    11581 cggctcgaaa caccgccctt tctacaacgt catcgttact gactcacgca gccgccgcga
    11641 cggccgcttc atcgaacgcg taggcttcta caaccccgta gccaacgaaa aacaagagcg
    11701 cgtccgcctc aacgcagacc gcctgaacca ctggattgca caaggcgcgc aagtcagcga
    11761 ctccgttgca aaactgatta aagaacaaaa agccgcctaa tccacatttg ccgccatgac
    11821 agacactcaa aaccgggtag ccatgggcta catcaaaggc gtattcggca taaaaggctg
    11881 gttgaaaatt gccgccaaca ccgaatattc cgacagcctt ttggactacc ccgagtggca
    11941 tttggtcaag gacggcaaaa ccatcagcgt tacccttgaa gccggaaaag tcgtcaacag
    12001 cgaactccaa gtcaaattcg aaggcataaa cgaccgcgac ttggcattct cattgcacgg
    12061 ttacaccatc gaaatacccc gtgaagcatt cgccccgaca gaagaagacg aatactactg
    12121 gacagacttg gtcggcatga ccgtcgtcaa caaagaccat accgttttag gcaaggtaag
    12181 caacctgatg gaaaccggcg caaacgacgt attgatgatt gacggagaac acgggcagat
    12241 tctgattccg ttcgtttccc aatatatcga aaccgtcgat accggcagca agattattac
    12301 tgccgactgg ggtttggact actgatgctt atccaggcag ttaccatttt ccccgaaatg
    12361 ttcgacagca ttacccgcta cggcgtaacg ggacgcgcga acagacaggg aatctggcag
    12421 tttgaagcag tcaatccccg aaagtttgcc gacaacagat tgggctacat cgacgaccgc
    12481 ccgttcggcg gcggcccggg aatgattatg atggctccgc cgcttcatgc agcaatagaa
    12541 cacgccaaag cacaatcttc ccaagctgca aaagtcattt acctcagccc ccaaggaaaa
    12601 ccgctgacac acccaaaagc ggcagaactg gcagaacttc cgcatctgat tctgctgtgc
    12661 ggacgctatg agggaataga cgaaagactg ctgcaaacca gcgtcgatga agaaatcagc
    12721 atcggagact tcgttgtttc cggcggagag cttcccgcca tgatgctgat ggatgcggta
    12781 ttgaggctcg tacccggcgt attgggcgat atgcagtctg ccgaacaaga ttcgttctca
    12841 agcggcattt tggactgccc ccactacacc aaacccttag aatttcaagg catggctgtt
    12901 ccggaagtat tacgttccgg caatcatggc ttgatagcgg aatggcggtt ggaacaatcg
    12961 ctgcgccgca ccttggagcg cagacccgat cttttggaaa agcgcgtttt aatcccaaag
    13021 gaatcccgcc tcttagaaac catccggcaa gagcaacggg aaatccaatc ataatttagg
    13081 aaaaaaacaa tgaacctgat tcaacagctc gagcaagaag aaattgcccg tctgaacaaa
    13141 gacatccccg aattcgcacc gggcgacacc gtagtcgtat ccgtacgcgt cgtggaaggt
    13201 acccgcagcc gtctgcaagc ctacgaaggc gttgtcattg cacgccgcaa ccgtggtctg
    13261 aacagcaact tcatcgtccg taaaatttcc agcggcgaag gtgtggaacg tactttccaa
    13321 ctgtactctc cgaccgtcga aaaaatcgaa gtcaaacgcc gtggcgacgt acgccgtgcc
    13381 aaactgtact acctgcgcgg cctgaccggt aaagctgcac gcattaaaga aaaactgcct
    13441 gcacgcaaag gttgattcaa accgttttcc cccaatgccg tctgaacctt cagacggcat
    13501 tttttatcgc cgcaatatca acaagacaac gatattccca atccgcctcc cctgcttaac
    13561 aagcactccc gtctattcaa acaaaattta cagaccaaac gttatacagt atcatagccg
    13621 ccatataacg ataaccgctt tgccaacttc cctttcaaaa ccgcaaatca ttatgacacc
    13681 ttccctctta ctgtcaggat tgaccttccg actcttcctc gccctgattg ccgtatccct
    13741 tttatggggc gtttaccttt gggcggtatc ggtatgagca tcatcgttga aaacctgacc
    13801 gtcagctacc gccgccgacc tgccgtgcac catgtggaca ttacttttga agaacatagt
    13861 atgtgggcgg ttttcggtcc caacggcgcg gggaaatcca cctttctcaa atccttaatg
    13921 ggattgcagc ctatcgatac gggcagcatc cggctggacg gattgacccg tcagaacatt
    13981 gcctaccttc cccagcagtc cgacatcgac cgctcgcagc ctatgaccgt tttcgacttg
    14041 gcggcaatgg ggctatggta tgaaatcggt tttttcagag gaataaatgc cgttcaaaaa
    14101 caacgcgttc acgaatcttt ggaacgcgtc gggatgcggc agtttgccgg ccgccaaatc
    14161 gcccacctct caaacggaca attccagcgt gtcctttttg cccgaatgct ggttcaaaac
    14221 gccaaattcc tacttctaga cgaacctttc aacgctgttg atacacggac gacctacgaa
    14281 cttctcgacg tattgcagaa atgccattgc ggcggacacg ccatcatcgc cgtactgcac
    14341 gattacgaac aagtccgtgc ctactttccc aataccctgc tgctcgcccg cgaaaaaatt
    14401 gcggcaggcg caaccgagac cattctgaca gaaccctacc tcgcccaagc caacgccaaa
    14461 atgcagcaac aggaaagccc cgagtggtgc gcctcataaa tgccgtctga aaccatgtcc
    14521 ccgtaccaaa cactatgaac ccctacgacc tgcttatcac accctttgca gaattcgact
    14581 ttatgcgcta cgccctcgca tccgtcttct gcctgtccct cagtgccgca cccgtcggcg
    14641 tattcctcgt catgcgccgt atgagcctga taggcgacgc attgagccac gccgtcctgc
    14701 ccggtgccgc cgtcggctac atgtttgccg gcttaagcct gcccgccatg ggtttgggcg
    14761 gcgtagccgc aggtatgctg atggcactgc ttgccggact cgtcagccgc ttcaccaccc
    14821 tgaaagaaga tgccaacttt gccgcctttt atctcagcag cctcgccata ggtgtagtcc
    14881 tcgtcagcaa aaacggcagc agcgtcgatt tgctccacct ccttttcggc tccgtacttg
    14941 ccgtcgatat tcctgccctg caactcatcg ccgccgtatc caccctcaca ctgcttaccc
    15001 ttgccgtcat ctaccgcccg ctcgtactcg aaagcatcga ccccctgttc ctcaaatccg
    15061 tcggcggcaa aggcgggctt tggcacgtac tctttctcgt cctggtcgtc atgaacctcg
    15121 tatccggctt tcaagccctc ggcacactca tgtccgtcgg actcatgatg ctgccagcca
    15181 ttaccgcccg cctgtgggcg aagcacatgg gcgcactcat cctcctatcc gttctgacag
    15241 ccctgctgtg cggcttgagc gggctgctca tttcctacca catcgaaatc ccttccggcc
    15301 ccgccatcat cctctgttgc agcgtccttt atctcttttc cgtcatactc ggcaaagaag
    15361 gcggcattct gaccaaatgg ctcaaaaacc accgccacca caccacctga ccccgcaagc
    15421 cgcttgaaca aacaggcggc ttgttttacc ataacagatg ttacgttata tcacaggaga
    15481 acaattatga aacaccccaa actcaccctt atcgccgcat tgctgaccac tgccgcaact
    15541 gccgcccccc tgccggttgt aaccagcttc agcattttag gcgacgtagc caaacaaatc
    15601 ggcggagagc gcgtatccat acaaagtttg gtcggagcca accaagatac gcacgcctat
    15661 catatgacca gtggcgacat taaaaaaatc cgcagtgcaa aactcgtcct gctcaacggc
    15721 ttaggacttg aagctgccga tgtacaacgt gccgtcaaac agagcaaagt atcctatgcc
    15781 gaagcgacca aaggcatcca acccctcaaa gccgaagaag aaggcggaca ccatcacgac
    15841 cacgatcatg accacgatca tgaccacgaa ggacaccacc atgaccacgg cgaatatgac
    15901 ccccacgtct ggaacgaccc cgtccttatg tccgcctatg cccaaaacgt cgccgaagcc
    15961 ctgataaagg ccgaccccga aggcaaagtt tattatcaac aacgtttggg caactaccaa
    16021 atgcagctca aaaaactgca cagcgacgca caagccgcat ttaacgccgt ccctgccgcc
    16081 aaacgcaaag tcctgaccgg gcacgatgcc ttttcctaca tgggcaaacg ttaccatatc
    16141 gaattcatcg ccccgcaagg tgtgagcagc gaagccgagc cttcagccaa acaagtcgcc
    16201 gccatcatcc gacaaatcaa acgcgaaggc atcaaagccg tctttaccga aaacatcaag
    16261 gacacccgta tggttgaccg tatcgccaaa gaaaccagtg tcaacgtcag cggcaaactg
    16321 tattccgacg cactcggcaa cgcgcccgca gacacctaca tcggaatgta ccgccacaac
    16381 atcaaagcct tgaccaacgc gatgaagcaa taaaaccgtt tgattggttt gaatttgaaa
    16441 aggcaaaagg ccgtctga
//
LOCUS       DAWFAN010000030        16441 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWFAN010000030 DAWFAN010000000
VERSION     DAWFAN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.030"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..303
                     /locus_tag="WGO65_000694"
     CDS             <1..303
                     /locus_tag="WGO65_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0327019.1"
                     /translation="KPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDV
                     TGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
     gene            310..385
                     /locus_tag="WGO65_000695"
     tRNA            310..385
                     /locus_tag="WGO65_000695"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Trp,seq:cca)
     gene            500..778
                     /gene="secE"
                     /locus_tag="WGO65_000696"
     CDS             500..778
                     /gene="secE"
                     /locus_tag="WGO65_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ0327020.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            780..1316
                     /gene="nusG"
                     /locus_tag="WGO65_000697"
     CDS             780..1316
                     /gene="nusG"
                     /locus_tag="WGO65_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ0327021.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1417..1851
                     /gene="rplK"
                     /locus_tag="WGO65_000698"
     CDS             1417..1851
                     /gene="rplK"
                     /locus_tag="WGO65_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ0327022.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1851..2546
                     /gene="rplA"
                     /locus_tag="WGO65_000699"
     CDS             1851..2546
                     /gene="rplA"
                     /locus_tag="WGO65_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ0327023.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2776..3276
                     /gene="rplJ"
                     /locus_tag="WGO65_000700"
     CDS             2776..3276
                     /gene="rplJ"
                     /locus_tag="WGO65_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ0327024.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3334..3705
                     /gene="rplL"
                     /locus_tag="WGO65_000701"
     CDS             3334..3705
                     /gene="rplL"
                     /locus_tag="WGO65_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ0327025.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3895..8073
                     /gene="rpoB"
                     /locus_tag="WGO65_000702"
     CDS             3895..8073
                     /gene="rpoB"
                     /locus_tag="WGO65_000702"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ0327026.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YGNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVVRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            8225..12400
                     /gene="rpoC"
                     /locus_tag="WGO65_000703"
     CDS             8225..12400
                     /gene="rpoC"
                     /locus_tag="WGO65_000703"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ0327027.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12691..12948
                     /locus_tag="WGO65_000704"
     CDS             12691..12948
                     /locus_tag="WGO65_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327028.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRIGMPINIG"
     gene            12945..13094
                     /locus_tag="WGO65_000705"
     CDS             12945..13094
                     /locus_tag="WGO65_000705"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0327029.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKISSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            13273..13644
                     /gene="rpsL"
                     /locus_tag="WGO65_000706"
     CDS             13273..13644
                     /gene="rpsL"
                     /locus_tag="WGO65_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ0327030.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13762..14232
                     /gene="rpsG"
                     /locus_tag="WGO65_000707"
     CDS             13762..14232
                     /gene="rpsG"
                     /locus_tag="WGO65_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ0327031.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            14251..16356
                     /gene="fusA"
                     /locus_tag="WGO65_000708"
     CDS             14251..16356
                     /gene="fusA"
                     /locus_tag="WGO65_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ0327032.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4349 a         3677 c         4277 g         4138 t
ORIGIN      
        1 aaaccgggta ctatcactcc tcacaccaag ttcaaagcag aagtatacgt actgagcaaa
       61 gaagagggcg gccgccatac cccgttcttc gccaactacc gtccccaatt ctacttccgt
      121 accaccgacg taaccggcgc ggttactttg gaagaaggtg tggaaatggt aatgccgggc
      181 gagaacgtaa ccatcaccgt agaactgatt gcgcctatcg ctatggaaga aggtttgcgc
      241 tttgcgattc gcgaaggcgg tcgtaccgtg ggtgccggcg tggtttcttc tgttatcgct
      301 taagtttaga ggccaatagc tcaattggta gagtatcggt ctccaaaacc gagggttggg
      361 ggttcgagac cctcttggcc tgccaaataa aaaattaacc ggccttgtgt cggttaattt
      421 ttttgtattt gttatttagt aaactctctt gccatttaca tggattgaga atagacagat
      481 gctatgatgg ataaataata tgacagaaca tacgcctgaa aaaaagaacg ttaaagtgga
      541 tcaactggtt gttcaagata aagaatctgc atctaattcc ggtaaggaag ggttttttgc
      601 atatttctca aattcttggt ccgaattcaa aaaggtggtt tggcctaagc gtgaagatgc
      661 tgtcagaatg actgtatttg ttatagtgtt tgttgctgtg ctttctatat ttatctatgc
      721 ggcagataca gcaatttcgt ggttattttt tgatgtattg ctgagaaggg aaggttgaga
      781 tgtcgaaaaa atggtatgtt gtacaggcgt attcggggtt tgagaagaat gtccaacgaa
      841 tattggaaga gcgcattgcc cgtgaggaga tgggagatta tttcggacaa attctggtgc
      901 ctgtagagaa agttgttgat atccgcaatg gtcgtaagac tattagtgaa agaaagtcat
      961 atcctggtta tgtgctagtt gagatggaaa tgacagatga ctcttggcat cttgtaaaaa
     1021 gcaccccccg tgtttccggt tttattggag ggagggctaa tagacctacg ccgattagtc
     1081 agagagaggc tgaaattatt ttacagcagg ttcagaccgg catagagaag ccgaaaccaa
     1141 aagttgaatt tgaggtcggt caacaggttc gtgtaaatga agggccgttt gcggatttta
     1201 acggggtggt tgaggaggtc aattatgaac ggaataagtt acgcgtgtct gttcagatat
     1261 ttggtagaga aacacccgtt gagctggagt tcagccaggt tgaaaagatt aactgatttt
     1321 tatacttgaa aaaaaagcaa taagaggata gaatcaaaaa ttaacttggg gagcggaaat
     1381 ggttccgcgt cttacccgtt tttaggagtt cgttaagtgg caaagaaaat tatcggctat
     1441 attaaactgc aaattcctgc aggtaaagcc aatccatctc ctccggttgg tcctgctttg
     1501 ggtcagcgcg gtttgaatat tatggaattt tgtaaggcat ttaatgctgc aacccaaggt
     1561 atggagcctg gcttaccgat tccggttgtg attactgcat ttgcagataa atcattcaca
     1621 tttgtgatga aaaccccgcc agcttctatc ttgttgaaaa aggctgccgg tttgcaaaaa
     1681 ggtagttcta atcctctgac caacaaagtg ggtaaattga cccgtgccca gttggaagaa
     1741 attgctaaaa ctaaagatcc tgatttgact gctgctgact tggatgcggc tgtccgtact
     1801 atagcaggtt ctgctcgctc aatgggcttg gatgtggagg gtgttgtata atggctaaag
     1861 tatctaaacg cttgaaagct cttcgctctt ctgtggaagc caataaatta tatgcaattg
     1921 atgaagcaat tgctttggta aaaaaagcag cgactgctaa atttgacgag tctgttgacg
     1981 tatctttcaa cttgggcgtt gatccgcgta aatctgacca agttatccgt ggttcggtcg
     2041 ttctgcctaa aggcaccggt aagataaccc gtgtggctgt atttactcaa ggtgcaaatg
     2101 cagaagctgc taaagaagct ggtgcagata tcgtcggttt cgaagatttg gctgctgaaa
     2161 tcaaagcagg caatctgaac tttgacgttg ttattgcttc tcccgatgca atgcgtattg
     2221 ttggtcaatt gggtaccatc ttgggtcctc gtggtctgat gccaaaccct aaagtaggta
     2281 cggttacccc taatgttgcc gaagcagtaa aaaatgcaaa agcaggtcaa gtgcagtacc
     2341 gtaccgacaa ggcaggtatt gttcatgcaa cgattggtcg tgcttcattc gctgaagctg
     2401 atttgaaaga gaactttgat gcgttgctgg atgctatcgt taaagccaag cctgctgccg
     2461 ctaaaggtca gtatctgaaa aaagttgctg tgtctagcac catgggtttg ggtattcgcg
     2521 ttgatacatc aagcgtaaat aactaatctt aaggaatttt caagcagttt ggttttctgg
     2581 gctgcttgaa tttgggctac ttaaaattaa gtagatgtcc aagaccgtag ggatcgtaag
     2641 atttaatcgt aactgcccta cgcagacggt agtcctgaaa cacattgcaa gattgcttgt
     2701 aagatgtctt tttaggttac cgcgctggtg ggatatcgtt ttggtatcct gtttataaac
     2761 agtgggaggt agaccttgag tctcaatatt gaaaccaaga aagtggcggt cgaggaaatt
     2821 agcgcggcaa ttgctaacgc tcaaaccctc gtagtcgctg aatatcgcgg tatcagtgtt
     2881 tccagtatga ctgagcttcg tgcgaatgca cgtaaagaag gcgtttattt gcgtgttctg
     2941 aaaaatactt tggctcgtcg tgcagtgcaa ggtacttcat ttgcagaatt ggccgatcaa
     3001 atggttggtc cgttggttta cgctgcttct gaagatgctg ttgctgctgc taaagtgttg
     3061 caccaattcg cgaaaaaaga tgacaaaatt gtcgttaaag ccggttctta caatggcgaa
     3121 gtaatgaatg ctgctcaggt tgctgagttg gcttctattc cgagccgcga agagctgttg
     3181 tccaaactgt tgttcgttat gcaagctcct gtatcgggct ttgcgcgcgg tttggctgct
     3241 ttggcagaga aaaaagccgg cgaagaagcc gcttaatcga ttttgtttct gttaatcaat
     3301 tattttttaa tacaatattt ggagtaaaat agcatggcta ttactaaaga agacattttg
     3361 gaagcagttg gttctttgac cgtaatggaa ttgaacgact tggttaaagc ttttgaagaa
     3421 aaattcggtg tttctgctgc tgctgttgca gttgcaggtc ctgctggtgc cggtgctgcc
     3481 gatgctgaag aaaaaaccga atttgatgtc gttttggctt ctgccggcga tcaaaaagtc
     3541 ggcgtgatta aagttgtccg tgcaattacc ggtttgggtc tgaaagaagc taaagacatc
     3601 gttgacggcg cacctaaaac cattaaagag ggtgtttcta aagctgaagc cgaagacatc
     3661 caaaaacaac tggaagaagc aggcgctaaa gtcgaaatca aataatttga tgcttcttat
     3721 ggaggctggc agttttctgc cagccttatt ttgcttctta aaataaacat caagtattgt
     3781 ttacatttat ttgcatagtt tttatcaagt cattgcaaat aaatgtaaat atcagattga
     3841 tgcgtaccgt tgtttcagac ggcctattat tgaaaattac ttttcggagt gtgtatgaac
     3901 tattcgttta ccgagaaaaa acgtatccgt aagagttttg caaagcggga aaatgttttg
     3961 gaagttcctt tcttgctagc aacccaaatt gattcttatg cgaagttttt gcagctggaa
     4021 aatgcttttg acaaacgtac cgatgacggt ctgcaggcag catttaattc tattttcccg
     4081 attgtgagcc ataacggtta tgcgcgattg gagtttgtgc attacacatt gggcgagcct
     4141 ttgttcgata ttcccgaatg tcagttgcgc ggaatcactt atgcagcccc cttgcgcgcg
     4201 cgtatccgtt tggtgatttt ggataaggaa gcatctaaac cgacggtaaa agaagttcgt
     4261 gaaaacgaag tgtatatggg cgaaattccg ttgatgaccc cgagcggttc ttttgtgatt
     4321 aacggcacag agcgtgtgat tgtctcccag ttgcaccgtt cgcccggcgt attcttcgag
     4381 catgacaaag gtaagacgca ctcttccggc aagttgttat tctccgcccg tatcattccc
     4441 taccgcggtt catggttgga ttttgaattt gacccgaaag atttgctgta tttccgtatc
     4501 gaccgccgcc gtaaaatgcc ggtaacgatt ttgttgaagg ctttgggcta cggcaatgag
     4561 caaatcttgg atattttcta cgacaaagaa acgttctatt tgtcttcaaa cggtgttcaa
     4621 accgatttgg tcgcaggccg tctgaaaggc gaaactgcca aggtcgatat cttggataaa
     4681 gaaggcaatg tattggttgc caaaggtaag cgcattactg cgaaaaatat ccgtgatatt
     4741 accaatgcag gcctgacccg tttggatgta gaaccggaaa gcctgctggg taaagcattg
     4801 gctgccgatc tgattgattc ggaaaccggc gaggtattgg cttctgccaa tgatgaaatt
     4861 acagaagagt tgttggccaa atttgatatc aacggcgtaa aagaaattac gaccctttac
     4921 atcaatgagt tggatcaggg tgcttatatc tccaacacct tgcgtacgga tgagactgcc
     4981 ggccggcagg cggcgcgtgt tgcgatttac cgtatgatgc gtccgggcga accgcccacc
     5041 gaagaggcgg tcgagcaatt gtttaaccgc ttgttcttca gtgaagacag ctacgatttg
     5101 tcccgcgtag gtcgtatgaa atttaatacg cgcacatacg aacaaaaact gtccgaagcc
     5161 caacaaaact cttggtacgg ccgcctgctg aacgaaacgt ttgccggtgc tgccgacaaa
     5221 ggcggttatg tcctgagcgt cgaagatatt gtcgcctcga ttgcgacttt ggtcgagttg
     5281 cgtaacggcc atggtgaagt tgacgatatc gatcacttgg gcaaccgccg agtacgttcg
     5341 gtaggcgagc tgactgaaaa ccaattccgc agcggtttgg cccgtgtgga acgtgctgta
     5401 aaagaacgtt tgaatcaggc ggaatcagaa aacttgatgc cgcacgattt gattaatgca
     5461 aaacctgttt ctgccgctat taaagaattc ttcggctcca gccaattgag tcagtttatg
     5521 gatcagacca accccttgtc tgaagtaacc cataaacgcc gcgtatccgc attgggtccg
     5581 ggcggtttga cccgcgaacg tgccggattt gaggtgcggg acgtgcatcc gacccactac
     5641 ggccgcgtat gtccgattga aacgcctgaa ggtccgaaca tcggtttgat caactcactg
     5701 tccgtttatg cgcgcaccaa tgattacggt ttcttggaaa cgccttaccg ccgcgttatc
     5761 gacggcaaag taaccgagga aatcgattac ttgtctgcca tcgaagaagg ccgctatgtg
     5821 attgcacagg cgaatgccga tttggattca gatggcaatc tgattggcga tttggttacc
     5881 tgtcgtgaaa aaggcgaaac cattatggca acgcccgacc gcgtccaata tatggacgtg
     5941 gcaaccggcc aggtggtatc cgttgcggca tccctgattc cgttcttgga acatgatgac
     6001 gcgaaccgcg cattgatggg tgccaacatg caacgtcagg cagtgccttg cttgcgtcct
     6061 gaaaaaccga tggtcggtac cggtatcgag cgttccgttg ccgttgactc tgctactgca
     6121 atcgttgccc gccgaggcgg cgtggtcgag tatgtcgatg ccaaccgcgt cgtcgttcgt
     6181 gttcatgacg atgaagcgac tgccggcgaa gtgggtgtcg atatttacaa cttggttaaa
     6241 tttacccgtt ccaatcaatc tactaacatc aaccaacgtc cggctgtcaa agcaggcgat
     6301 gttttgcaac gcggcgattt ggtggccgat ggcgcgtcca ccgatttggg cgaattggct
     6361 ttgggtcaaa atatgaccat cgccttcatg ccgtggaacg gttacaacta cgaagactcg
     6421 attctgattt ccgaaaaagt ggctgcggac gaccgctata cttcgattca cattgaggaa
     6481 ttgaatgtcg ttgcccgcga taccaagctg ggtgcggaag acattacccg cgatattccg
     6541 aacttgtccg agcgtatgca aaaccgtttg gacgaatccg gtatcgttta catcggtgcg
     6601 gaagtagaag ccggcgatgt gttggtaggc aaggtaacgc ctaaaggcga aacccagctg
     6661 acgccggaag aaaaactgct gcgcgccatc ttcggtgaaa aagcatctga cgtaaaagat
     6721 acttcattgc gtatgcctac cggcatgagc ggtaccgtta tcgacgttca agtcttcact
     6781 cgtgaaggta ttcaacgcga caaacgtgct caatccatta tcgattccga gttgaaacgc
     6841 taccgtttgg atttgaacga ccaattgcgt attttcgaca acgacgcatt cgaccgtatc
     6901 gagcgtatga ttgtcggtca gaaagccaac ggcggcccga tgaagctggc caaaggcagt
     6961 gaaatcacga ccgaatatct ggcgggtctg ccgagcaggc acgattggtt cgatatccgt
     7021 ctgaccgatg aagatttggc caagcagttg gaactgatta aagtgagcct gcaacaaaaa
     7081 cgcgaagaag cggacgagtt atacgaaatc aagaagaaaa aactgaccca aggcgacgaa
     7141 ttgcaacccg gcgtacaaaa aatggtgaaa gtttttatcg ccatcaaacg ccgtctgcaa
     7201 gccggcgaca aaatggcggg ccgccacggt aacaaaggcg tggtatcgcg cattctgcca
     7261 gtggaagaca tgccttacat ggcggacggc cgtccggtag acatcgtact gaacccattg
     7321 ggcgtacctt cccgtatgaa catcggtcag attttggaag ttcacttggg ttgggcagca
     7381 aaaggtatcg gcgagcgtat cgaccgtatg ctgaaagagc aacgcaaagc aggcgagttg
     7441 cgcgagttct tgaacagact ctacaacggc agcggtaaga aagaagattt ggatgccctg
     7501 actgatgaag aaatcatcga actggcctcc aacctgcgca aaggtgcatc tttcgcctct
     7561 cctgtattcg acggtgcgaa agagtctgaa atccgcgaaa tgctgaactt ggcttatccg
     7621 agcgacgatc ctgaggttga aaaactgggc ttcaacgaca gtaaaaccca aatcacgctg
     7681 tatgacggcc gttcaggcga agcatttgac cgcaaggtta cagtaggtgt gatgcactat
     7741 ctgaaactgc accacttggt tgacgaaaaa atgcacgccc gttctaccgg tccgtacagt
     7801 ctggttaccc agcagccttt gggcggtaaa gcccagttcg gtggccaacg tttcggcgag
     7861 atggaggttt gggcattgga agcatacggc gcggcataca cgctgcaaga gatgttgact
     7921 gtgaagtctg acgacgtgaa cggtcgtacc aaaatgtacg aaaacatcgt caaaggcgaa
     7981 cacaaaatcg atgccggtat gcccgagtcc ttcaacgtat tggtcaaaga gattcgctca
     8041 ctgggcttgg atatcgattt ggaacgctac tgatacgggt ttcagacggc ataaggggag
     8101 ctgttctgca ggtatgcggg gcagccgaca atgtttaaaa acgaaatgcc gtctgaaaac
     8161 actgtacctc tatccatatc gaaaatccgc catgcggtaa aaatacttcc ttcaaggagc
     8221 aaaaatgaat ttgttgaact tatttaatcc gttgcaaact gccggcatgg aagaagagtt
     8281 tgatgccatc aaaatcggta ttgcctctcc cgaaaccatc cgctcatggt cttatggcga
     8341 agtcaaaaaa cctgaaacca tcaactaccg tacgttcaaa cctgagcgtg acggtttgtt
     8401 ctgtgccaaa atctttggcc cggtcaaaga ctacgaatgc ttgtgcggaa aatacaaacg
     8461 cttgaaattt aaaggtgtaa cttgtgaaaa atgcggcgtg gaagtaaccc tgtccaaagt
     8521 gcgccgcgaa cgcatgggcc atatcgaatt ggctgcgccc gtcgcacata tttggttctt
     8581 aaaatccctg ccttcccgct tgggtatggt gttagacatg actttgcgcg atatcgaacg
     8641 cgtattgtac tttgaagcat ttgtggtaac cgatcccggt atgactccgc tgcaacgccg
     8701 ccaattgctg actgaagacg attactacaa caagctggac gaatacggcg acgatttcga
     8761 tgccaaaatg ggtgcggaag gtatccgcga attgctgcgt accctgaatg tagcgggcga
     8821 aatcgaaatc ctgcgccaag agttggaatc gaccggttcc gacaccaaaa tcaaaaaaat
     8881 cgccaaacgc ttgaaagtat tggaagcttt ccatcgttcc ggtatgaaac tggaatggat
     8941 gattatggat gtgctgccgg tattgccgcc tgatttgcgt ccgttggttc cattggatgg
     9001 tggtcgtttt gccacttccg atttgaacga tttgtaccgc cgcgttatta accgtaacaa
     9061 ccgtctgaaa cgtctgttgg aactgcatgc tcctgacatc atcgtccgca acgaaaaacg
     9121 tatgttgcaa gaagcagttg actcgctgtt ggataacggc cgtcgcggta aagccatgac
     9181 cggcgccaac aaacgcccgc tgaaatcatt ggcagacatg attaaaggta agggtggccg
     9241 cttccgtcaa aacctgctgg gcaaacgtgt ggactactcc ggccgttccg tgattaccgt
     9301 aggcccgtac ctgcgtctgc accaatgcgg tttgccgaaa aaaatggctt tggaactgtt
     9361 caaaccgttc attttccaca aattggaaaa acaaggtttg gcctctaccg ttaaagcagc
     9421 gaaaaaattg gtagagcaag aagtaccgga agtatgggac atcttggaag aagtcatccg
     9481 cgaacatccg attatgctga accgtgcgcc gaccctgcac cgtttgggta ttcaagcgtt
     9541 cgaacctatc ttgattgaag gtaaagcgat tcagttgcac ccattggtgt gtgctgcgtt
     9601 caacgccgac tttgacggcg accaaatggc ggtacacgtt ccattgagct tggaagcaca
     9661 aatggaagca cgcacgctga tgctggcttc aaacaacgta ttgtctcctg ccaacggcga
     9721 accgattatc gtaccttccc aagacatcgt attgggtctg tactacatga cccgcgaccg
     9781 tatcaatgcc aaaggcgagg gcagcctgtt tgccgatgtg aaagaagtgc atcgcgcata
     9841 ccataccaaa caggttgaac tgggtacgaa aattaccgta cgtctgcgcg aatgggtgaa
     9901 aaacgaagca ggtgagtttg agcctgtcgt taaccgttac gaaacaaccg tcggccgtgc
     9961 attgttgagc gaaatcctgc cgaaaggcct gccgtttgaa tatgtcaaca aagcgttgaa
    10021 gaaaaaagaa atttctaaac tgattaacgc atcgttccgc ctgtgcggct tgcgcgatac
    10081 ggttatcttt gctgaccacc tgatgtacac cggtttcgga tttgcggcaa aaggcggtat
    10141 ttccattgcc gttgacgata tggaaattcc aaaagaaaaa gcggccttgc tggctgaagc
    10201 caatgccgag gttaaagaaa tcgaagacca ataccgtcaa ggtttggtta ccaacggcga
    10261 acgctacaac aaggtggtcg atatttgggg tcgtgccggc gataaaatcg ctaaagcgat
    10321 gatggacaac ctgtccaaac agaaagttat cgaccgtgcc ggcaacgaag tcgatcaaga
    10381 gtcattcaac tccatctata tgatggctga ctccggtgcc cgtggttctg cggctcagat
    10441 taaacagttg tccggtatgc gcggtttgat ggcgaaacct gacggctcga ttattgaaac
    10501 gccgattacc tcaaacttcc gcgaaggtct gaccgtattg caatacttta ttgcgaccca
    10561 cggtgcgcgt aagggtttgg cggataccgc attgaaaacc gcgaactccg gttacctgac
    10621 ccgtcgtctg gtagacgtaa ctcaagattt ggtcgttgtt gaagacgatt gcggtacttc
    10681 agacggcttt gtcatgaagg cagtggtaca aggcggtgat gtgattgaag cattgcgcga
    10741 tcgtattttg ggccgtgtta ccgcgtctga cgttgtcgat ccgtcaagtg gcgaaacatt
    10801 ggttgaagca ggcacgttgt tgactgaaaa actggtggat atgattgacc aatccggtgt
    10861 cgatgaagtc aaagtccgta cgccgattac ttgtaaaacc cgtcacggtt tgtgcgcgca
    10921 ctgttacggc cgcgacttgg cgcgcggcaa actggtcaat gccggcgagg cagtcggcgt
    10981 gattgccgct cagtctatcg gcgaaccggg tacccagttg accatgcgca cgttccacat
    11041 cggtggtgcg gcatcccgtg cggcagcagc cagccaagtg gaagccaaat ccaacggtac
    11101 ggcacgtttc agcagccaga tgcgttacgt tgccaacaac aaaggtgagt tggttgtcat
    11161 cggtcgctct tgcgaagtcg tgattcatga tgacatcggc cgtgaacgcg aacgccacaa
    11221 agtaccttac ggtgccatcc tgctggtaca agacggtatg gccattaaag ccggtcaaac
    11281 cttggcaacc tgggatccgc atacccgtcc gatgatcacc gaacatgcag gtatggtgaa
    11341 atttgagaac gtggaagagg gtgttaccgt tgcgaaacaa actgacgatg taaccggttt
    11401 gtccactttg gtggtgattg acggtaaacg ccgttccagc agcgcgtcca aactgctgcg
    11461 tccgactgta aaattgttgg atgaaaacgg cgtggaaatc tgtattcccg gtacgtccac
    11521 tccggtttcg atggcattcc ccgttggtgc ggtgattacc gtacgcgaag gtcaggaaat
    11581 cggtaaaggc gacgtattgg cgcgtatccc gcaagcctct tccaaaaccc gcgacattac
    11641 cggtggtctg ccgcgcgttg ccgaattgtt tgaagcacgc gtgccgaaag atgccggtat
    11701 gttggcggaa attaccggta ccgtttcctt cggcaaagag accaaaggca agcaacgtct
    11761 gattgttact gacgtggacg gtgtagcata cgagaccttg atttccaaag agaaacaaat
    11821 tctggtacac gacggtcaag tggtaaaccg cggtgaaacc atcgtggacg gagctgttga
    11881 tccgcatgat attctgcgtt tgcaaggtat cgaagcactg gcacgctaca ttgtccaaga
    11941 ggtgcaagag gtttaccgtc tgcaaggtgt gaagatttct gataaacaca tcgaagtcat
    12001 catccgtcaa atgttgcgcc gtgtgaacat tgcggatgcc ggcgaaaccg ggttcattac
    12061 cggagagcag gtcgaacgcg gcgatgtgat ggcggccaat gaaaaagctt tggaagaagg
    12121 caaagagccg gcacgttacg aaaacgtatt gctgggtatt accaaagcct ccctgtccac
    12181 cgacagtttc atttctgccg catcgttcca agaaacgacc cgtgttctga ccgaagccgc
    12241 gattatgggc aaacaagacg aactgcgcgg tttaaaagag aacgtcatcg tcggccgcct
    12301 gattcctgcc ggtaccggtt tgacttatca ccgcagccgt catcagcaat ggcaaggggt
    12361 agagcaggag actgctgaaa cccaagtaac ggatgaataa tcttcggcgc gtttattcaa
    12421 tacaaaaccg caagctttaa gcttgcggtt tttctttgtc cgattaaggc aaaaacaagc
    12481 gttttcgtca ttttgaggcg tgtggattat tccttaggta ttttcgggcc ggagaccaac
    12541 gaggtggcgg gtgtcgtcgg tacgtccgga gaccaaaata actttgccaa ggatgttggt
    12601 ttcggcggtc aaaaaagtag cgtcttaatg ttttccattt aaacaaatgt cgtctgaaac
    12661 ttcagacggc atttccttta agaaataaat atgaaaccca gaaatctctt ttttgcaggc
    12721 tgcctgctga cttcggcgac gtttgccgag gatatcggcg tacctgtcga actgattaac
    12781 gtcggtaatc ggattgcgat gccgtctgaa ggggaaagcc tcgccctcct gccgtttgcc
    12841 gaggatgtac cgccggttcg cgatgcaatg ccgtctgaag ttcctaaaag cgcggcaggc
    12901 ggcgatgttc ggggtgaccg gataggaatg ccgattaaca tcggatgagc gcggctttat
    12961 ggcataaaaa actgtcgtgg aaaggattta caccccaaat aaatttccgt tacaacaaga
    13021 tcagcagcaa tatgcccgcc ttttattcgc gcagcggcaa ggaacggttt gtcagtatag
    13081 aaaaaacgta ttgacagtat tttcttcagt cgtccgactg attgtgaggg atgtcggtaa
    13141 atatttatcg gcaaacaaga aaatcatctt tcttcttgtc gttatgcttg actgtatact
    13201 tgcaataaaa atataattcc actcttgccg acatggtgtc ggcaagtatt taactcaaca
    13261 ggacgagaaa atatgccaac tatcaaccaa ttggtacgca aaggccgtca aaagcccgtg
    13321 tacgtaaaca aagtgcccgc actggaagcc tgcccgcaaa aacgcggcgt gtgcacccgt
    13381 gtatacacga ctacccctaa aaaacctaac tctgcattgc gtaaagtatg taaagtccgc
    13441 ctgaccaacg gttttgaagt catttcatac atcggcggcg aaggccacaa cctgcaagag
    13501 cacagtgtcg tattgattcg cggcggtcgt gtaaaagact tgccaggtgt gcgttaccac
    13561 actgtacgcg gttctttgga tactgcaggc gttaaagacc gtaaacaagc ccgttccaaa
    13621 tacggtgcta agcgtcctaa ataattactg ggacttaaat aggcacgtcg gccgcctaag
    13681 ctgaacaacg gccgagtaag tgaatactca attgggtatt catgggaata gacccgactg
    13741 aatagattaa aggaaattaa aatgccaaga cgtagagaag tccccaagcg cgacgtactg
    13801 ccagatccta aattcggcag cgtcgagttg accaaattca tgaacgtatt gatgattgac
    13861 ggtaaaaaat ccgttgccga gcgtatcgtt tacggtgcgt tggaacagat tgagaaaaaa
    13921 accggcaaag tagcaatcga agtatttaac gaagccattg caaacgccaa acctatcgtg
    13981 gaagtgaaaa gccgccgtgt aggtggtgca aactaccaag ttcctgttga agttcgtcct
    14041 tcacgccgtt tggctttggc aatgcgctgg gttcgcgatg cggcccgcaa acgtggtgag
    14101 aaatccatgg acctgcgttt ggcaggcgaa ttgattgatg cgtccgaagg ccgtggcggt
    14161 gcgttgaaaa aacgtgaaga agtacaccgt atggctgaag ccaacaaagc attctctcac
    14221 ttccgtttct aattttgaaa ggctaataaa atggctcgta agaccccgat cagcctgtac
    14281 cgtaacatcg gtatttccgc ccatatcgat gcgggtaaaa ccacgacgac agaacgtatt
    14341 ttgttctata ccggtttgac ccacaagctg ggcgaagtgc atgacggtgc ggctactacc
    14401 gactacatgg aacaagagca agagcgcggt attaccatta cctccgctgc cgttacttcc
    14461 tactggtccg gtatggcgaa acaattcccc gagcaccgct tcaacatcat cgacaccccg
    14521 ggacacgttg actttaccgt agaggtagag cgttctatgc gtgtattgga cggcgcggta
    14581 atggtttact gcgcggtggg cggtgttcaa ccccaatctg aaaccgtatg gcggcaagcc
    14641 aacaaatacc aagtgccgcg cttggcgttt gtcaataaaa tggaccgtca gggtgccaac
    14701 ttcttccgcg ttgtcgagca aatgaaaacc cgtttgcgcg caaaccctgt acctatcgtc
    14761 attccggttg gtgcggaaga caacttcagc ggtgtggttg atttgttgaa aatgaaatcc
    14821 atcatttgga atgaagccga taaaggtaca acctttacct atggcgatat tcctgccgaa
    14881 ttggtcgaaa ctgccgaaga atggcgtcaa aatatgattg aagccgcagc cgaagccagc
    14941 gaagaactga tggacaaata cttaggcggc gacgagctga ccgaagaaga aatcgtaggc
    15001 gcgttgcgtc aacgtacttt ggcaggcgaa attcagccta tgctgtgtgg ttctgcattt
    15061 aaaaacaaag gtgttcaacg tatgttggac gcagttgtag aattgctgcc agctcctacc
    15121 gatattcctc cggttcaagg tgtcaacccg aataccgagg aagccgacag ccgtcaagcc
    15181 agcgatgaag agaaattctc tgcattggcg ttcaaaatgt tgaacgacaa atacgtcggt
    15241 cagctgacct ttatccgcgt ttactcaggc gtagtaaaat ccggcgatac cgtattgaac
    15301 tccgtaaaag gcactcgcga acgtatcggt cgtttggtac aaatgactgc cgcagaccgt
    15361 actgaaatcg aagaagtacg cgccggcgac atcgcagccg ctattggtct gaaagacgtt
    15421 actaccggtg aaaccttgtg tgcggaaagc gcgccgatta tcttggaacg catggaattc
    15481 cccgagccgg taatccatat tgccgttgag ccgaaaacca aagccgacca agagaaaatg
    15541 ggtatcgccc tgaaccgctt ggctaaagaa gacccttctt tccgtgtccg tacagacgaa
    15601 gaatccggtc aaaccattat ttccggtatg ggtgagctgc acttggaaat tattgttgac
    15661 cgtatgaaac gcgaattcgg tgtggaagca aatatcggtg cacctcaagt ggcttaccgt
    15721 gaaactatcc gcaaagccgt taaagctgaa tacaaacatg caaaacaatc cggtggtaaa
    15781 ggtcaatacg gtcacgttgt gattgaaatg gaacctatgg agccgggtgg tgaaggttac
    15841 gagtttatcg atgaaattaa aggtggtgtg attcctcgcg aattcattcc gtctgtcgat
    15901 aaaggtatcc gcgatacgtt gcctaacggt atcgttgccg gctatcctgt agttgacgta
    15961 cgtatccgtc tggtattcgg ttcttaccat gatgtcgact cttcccaatt ggcatttgaa
    16021 ttggctgctt ctcaagcgtt taaagaaggt atgcgtcaag catctcccgc cctgcttgag
    16081 ccgattatgg cagttgaagt ggaaaccccg gaagaataca tgggcgacgt aatgggcgac
    16141 ttgaaccgcc gtcgcggtgt tgtattgggt atggatgatg acggtatcgg cggtaaaaaa
    16201 gtccgtgccg aagtacctct ggcagaaatg ttcggttact cgaccgacct gcgttctgca
    16261 acccaaggcc gcgctactta ctctatggag ttcaagaaat attctgaagc tcctgctcac
    16321 atagctgctg ctgtaactga agcccgtaaa ggctaatcag gcaaataggc cgtccgaaag
    16381 gctgaaatga tttttcagac ggcattgttc tttaatcgat ctttaatgta aaggaattag
    16441 c
//
LOCUS       DAWFAN010000031        16331 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWFAN010000031 DAWFAN010000000
VERSION     DAWFAN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.031"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(163..2952)
                     /gene="ileS"
                     /locus_tag="WGO65_000709"
     CDS             complement(163..2952)
                     /gene="ileS"
                     /locus_tag="WGO65_000709"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ0327033.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLASIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETPRVADMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCENYDKLSDTMDVWFDSGSTHYSVVKQREKLEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
     gene            complement(3093..4013)
                     /gene="ribF"
                     /locus_tag="WGO65_000710"
     CDS             complement(3093..4013)
                     /gene="ribF"
                     /locus_tag="WGO65_000710"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ0327034.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKRW"
     gene            complement(4540..5835)
                     /gene="tyrS"
                     /locus_tag="WGO65_000711"
     CDS             complement(4540..5835)
                     /gene="tyrS"
                     /locus_tag="WGO65_000711"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ0327035.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLYQKQVFG
                     LTLPLVTKSDGIKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(5898..7688)
                     /locus_tag="WGO65_000712"
     CDS             complement(5898..7688)
                     /locus_tag="WGO65_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0327036.1"
                     /translation="MAAGRISRCRYILCDFRLFDDGDPPSRNVGGGGGFSLKAFYIRR
                     IKRILPAFFAVLAATLAGGFFLFTKDDFFLLWKSALTALGFASNLYFARGKDYFDPAQ
                     EEKPLLHIWSLSVEEQFYFVFPILLLLVARKSPRVQFGFLAALCALSLAASFMPSAFD
                     KYYLPHLRACEMLVGSLTAVWMRYRQQRNLAVGRCCAAAGALFSACILSACLFAYSEQ
                     TAYFPGPAALIPCLAVAALIYFNHYEHPLKKFFQWKITVAAGLISYSLYLWHWPILAF
                     MRYIGPDNLPPYSPAAAVVLILLLSLFSYHCIEKPFKKWQGSFAQSVLWIYALPMLIL
                     GAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELLVLGDSHADHY
                     KTFFDAVGKKEKWAATMVSADACAYVEGYASRVFQNWAACRAVYRYAEEHLPRYSKVV
                     LAMRWGSQMPENSRSLAYDAGFFKKFDRMLHKLSSEKQAVYLMADNLASSYNVQRAYI
                     LSSRIPGYRQTLRPDDESTLKANARIRELAAKYPNVYIIDAAAYIPANFQIGGLPVYS
                     DKDHINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            complement(7788..7892)
                     /locus_tag="WGO65_000713"
                     /pseudo
     CDS             complement(7788..7892)
                     /locus_tag="WGO65_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(8144..9235)
                     /gene="ychF"
                     /locus_tag="WGO65_000714"
     CDS             complement(8144..9235)
                     /gene="ychF"
                     /locus_tag="WGO65_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ0327037.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(9349..11025)
                     /locus_tag="WGO65_000715"
     CDS             complement(9349..11025)
                     /locus_tag="WGO65_000715"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ0327038.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAVLLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGVGGADLARKVVNAIENQPNNFNFSYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSSGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            11200..11631
                     /locus_tag="WGO65_000716"
     CDS             11200..11631
                     /locus_tag="WGO65_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0327039.1"
                     /translation="MSSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGKNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(11862..12557)
                     /locus_tag="WGO65_000717"
     CDS             complement(11862..12557)
                     /locus_tag="WGO65_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ0327040.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(12573..13073)
                     /gene="hpaC"
                     /locus_tag="WGO65_000718"
     CDS             complement(12573..13073)
                     /gene="hpaC"
                     /locus_tag="WGO65_000718"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ0327041.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVTP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            complement(13188..13628)
                     /gene="nadR"
                     /locus_tag="WGO65_000719"
     CDS             complement(13188..13628)
                     /gene="nadR"
                     /locus_tag="WGO65_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ0327042.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(13971..14480)
                     /locus_tag="WGO65_000720"
     CDS             complement(13971..14480)
                     /locus_tag="WGO65_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ0327043.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(14559..14634)
                     /locus_tag="WGO65_000721"
     tRNA            complement(14559..14634)
                     /locus_tag="WGO65_000721"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14599..14601),aa:Lys,seq:ttt)
     gene            14845..15924
                     /gene="apbC"
                     /locus_tag="WGO65_000722"
     CDS             14845..15924
                     /gene="apbC"
                     /locus_tag="WGO65_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ0327044.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQHSDGIHIALHF
                     GFPVAHIAAAIADSVQETLMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSGGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGAAAQLFDKHPAIARIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
BASE COUNT         3709 a         4448 c         4398 g         3776 t
ORIGIN      
        1 cgagattcta gattcccact ttcgtgggaa tgacggttca gttgcattcc gacaacaccg
       61 caatcttgaa acccctccgc cgttataaag acaaatcgcg gcacaaaaaa tgccgtctga
      121 aatgctgttc ggcggtttca gacggcattt gctcaaactt tatcaggcgt aatggcgcgt
      181 ttcgccttct ccgccgacat tctctgcaca gcgtttgcag acggtttcat agcctgcaac
      241 cgcgcccaca tcgcgggtgt agtgccagca gcgttcgcat ttttcaccat cactggcttt
      301 agcggcaacg gcaagttcgc tgcctacttt cacttctgct ttagacacca gcaaagcaaa
      361 gcgcaattct tcgcccaaag cattcagata gccggccatt tcttccggcg cggtaatttc
      421 ggcttcggct tgcaaggacg aaccgacggt tttgtcggcg cgcaaaggct cgatggcggc
      481 ggttaccgct tcgcgggctt cgcggattgc cgtccatttt ttcaccagtt cggcttcggt
      541 tttttcgttg atggtcggga actcgtgcca agtatggaag aggacgctgt cttcttcgcc
      601 gccgccgatg atgtcccacg cttcttcgcc ggtgaagcac aaaatcggtg caatcaagag
      661 aaccaaactg cgtgtgatgt gatacagggc agtttgtgcg ctacggcgtg catggctgtc
      721 ggctttggtg gtgtagaggc ggtctttcag gatgtcgagg tagaacgcac ccaagtcttc
      781 cgagcagaaa gaaacaatgt cttttacggc aaagtggaag gcataacgcg gatagtaatc
      841 gcctgccaga cgctcttgca gctgacgtgc caataccacg gcgtagcggt cgatttccac
      901 catatccgcc tgttgcacgg catcttcaat cggattaaag tcgctcaagt tggcaaacaa
      961 aaagctcaag gtattgcgga tacggcggta gctttcggtt acgcgtttga ggatttcttt
     1021 ggaaatcgcc aattcgccgc tgtaatcggt agatgccgcc cacaggcgca ggatgtctgc
     1081 gccgaattcg ttataaacct cttgcggtgc aacgacgttg ccgatggatt tcgacatttt
     1141 tttgccttcg ccgtcgacaa cgaaaccatg ggtcagcagc tgtttatacg gcgcgcgacc
     1201 cattgatgag gcgcagccgg tcagcatgga cgattgaaac cagccgcggt gttggtcgct
     1261 gccttcgaga tacaaatcag ccggccattc caatttttcg cgttgtttca caacggaata
     1321 atgggtcgag ccggagtcga accatacgtc cattgtgtca gaaagtttat cgtaattttc
     1381 gcaatcttcc gcgctcaaga gttcgctctt atcgagggag aaccacgctt cgatgccttt
     1441 ttcttcgatt ttcagggcaa ctttttccaa aagttcggca gagttcggat gcagctcgcc
     1501 cgtttctttg tgaacaaaga aagtcatcgg cgtgccccaa tagcgttggc gtgaaaccac
     1561 ccagtcagga cgaccttcaa tcatggcttc caaacgcgcg cgaccccaag acgggaagaa
     1621 ttcggtgtcg tccacggcct tgatggcttt gtcgcgcagg gttttgccgt cggcaccagc
     1681 tttgtccata ccgacaaacc attgacctgt cgcgcggtaa atcagcggcg ttttgtgccg
     1741 ccagcagtgg gcgtagctgt gttcgatttt actgcttgcc aaaaggttgc cggtttcttc
     1801 caaccattgc aggatgacgg ggttcgcctc ccaaacgcgc atatcggcaa cgcgcggcgt
     1861 ttcgccgatg tatttgcctt cggcgttgac aggattgtaa agctcgatgc cgtatttgtt
     1921 gcagacggcg tagtcttcca aaccgtgcgc gggcgcagta tgcaccaagc cggtaccggc
     1981 atcggtggta acgtgttcgc cgttaagcat gggaatatcg cgttcgagga acggatggtt
     2041 catgtgcagg ttttccagct tgtcgccggt ggtttcggca aggatggcga tgccgtctga
     2101 aaagccgtaa cgtttgagcg cgtcttccgc caaatctttc gccaatacca atttgccttt
     2161 gggcgtatca atcagttgat acaccacgtc cgcgccggca gatacggctt ggctcgcagg
     2221 cagcgtccaa ggcgtagtcg tccagatgac ggcaaacgct ttgccttcga tacttgccaa
     2281 accgaatgcg gcggcaagcg cggcagtgtc tttaaacgga taggcaacgt caatcgcggg
     2341 cgagattttg tctttatatt ccacttccgc ttcggccagc gaagaaccgc agtccaagca
     2401 gaattgaacc ggtttcgcac cccggtagag atagccggat ttgtagattt cgccgagcat
     2461 acgcacggta tcggcttcgg ttttgaaatc catagtcagg taaggatggt cccagtcgcc
     2521 caacacgccc aagcggataa agtctttttt ctgacgggca atctgttcgg cggcgtattc
     2581 gcggcacaat tcgcggaagc gggctttggg catgtctttg ccgtgcagct tttccaccat
     2641 cacttcgatg ggcagaccgt ggcagtccca acccggcaca taaggcgcat cgaaaccggc
     2701 ttgggttttg ctgcggataa tgatgtcttt aaggatttta ttgacggcgt gaccgatgtg
     2761 gatgtcgccg ttggcatacg gaggaccgtc gtgcagaatg aatttcgggc ggcctttggc
     2821 gatttcgcgc agtttttggt agcgtttttg ctcgtaccag cttttcagcc atgcaggctc
     2881 gcgcttggca agattgccgc gcattggaaa cgggctctcg agcaggttga cggttttgct
     2941 gtaatcggtc attttttaat ctctattgtt acaatatttc ggttcagacg gcattgcgcc
     3001 tcaaacagta tttcacaacg ggaaacccct atgccgtctg aaaagttaaa agattgattg
     3061 tagcccaatc ggatggtttg tataaggttt ttctaccaac gctttgcggc ttccatatcg
     3121 gcttcaatct gccttttcag ttcttccata ccgtcaaact tttcctcatc gcgcagtttg
     3181 tgcaggaagc ggacgttcag ccgttgtccg tacaggtcgc cttgaaagtc gaacaggtgg
     3241 acttcaagct tttgagaaca gccgctatca acggtgggat tgaagccgaa gctcgccacg
     3301 ccgcgccgcg tgccgaatgc gccgtccgct tcgacgacga acacgccgcc gagtgcataa
     3361 cggtggcggg gcaggcggat gttggcagtc ggggcgttta aggtgcgtcc gagttttctg
     3421 ccgtgcacca ccctgccgct caagacgtag tcgtgtccca aaagtttttt cgcataggca
     3481 aggttgccgt ctgaaagggc ttgtcgcacg gcggtactgc tggtgcggat gtcttcgacg
     3541 atgacggaag gcgtacgctc ggtctgcata tcgggctgtt gtgccaaaag ttcaaaacag
     3601 ccttcccgcc ccgcaccgaa acggaaatca tcgccgacga gcaaataacg cgtattcaag
     3661 gtttgacgca gcaggcggtc gataaaccct tgcgcggata tttcggaaaa attttgatcg
     3721 aaacgcaaaa cccagacggc atcgacacag cctgtgcctt ccaataattc gagcttggtg
     3781 cgcagggggc tgatccgaca cggtggcatc ctgccggtgc ggagtgcgaa aaattctttg
     3841 ggttggggtt cgaaaacgac ggtcacgacg ggtaatccgc gcgtatcggc ttcgaggcgg
     3901 agtttttgga ggatgtgttt gtgtccgagg tgtacgccgt caaaattgcc tatggttacg
     3961 gcggccccgt gcggaaagtc gggcgcgttg tgccgcccca gcctgattct cattgttatg
     4021 ttcgggtatg tggtgaaaca ggcggtcatt gtaaacggta ttgcggttta tagacagtgt
     4081 gtcgcccgtt acgcccgccc gacgcgggaa aagtaggcaa atttcccgcc gccgaacgcg
     4141 ccaaacgcat aaagcgcgca gcaggcgcgg tgctatgtgt tgaaacatcg ccccaaacct
     4201 gatgcaacat cagtgaaaat cgttcttttt taaccaggta aaccgagaca aacaaccctc
     4261 cgccgtcatt cccgcgcagg cgggaatccg gacctgtccg cacagaaact tatcggataa
     4321 aaacagttgc tcaaaccccg agattctaga ttcccacttt cgtgggaatg acggttcagt
     4381 tgcgttccga caacaccgta atcttgaaac tcgtcattcc cgctcaggcg ggaatctaga
     4441 acgtggaatc taagaaaccg ttttgcccga taagtttccg tgcggacagg tccggattcc
     4501 cgcctgcgcg ggaatgacgg catttctgcg gcaatcggat tatttccaaa ccaaaagcgc
     4561 gtggttgcgt ttgccgcgcc gaaggatggt gtatttgccg aaacgtttgt gttcgtcggt
     4621 cagcaggcag gcatcgtcgg ggcgttcggc ggcgtggttg gggttgttgg cttcggcagg
     4681 tttgccgttg agcaaaaccg ctttgctgtt cacaaagccg cgcgcttctt tattggagga
     4741 tgccaaaccg gttttgacca aggcttcgac gacattgatg ccgtctgaaa cttcaaatgc
     4801 gggcaggccg tcgagtgcca gctgctcgaa gtcgctttcg gtcaggctgc tttggtcttc
     4861 ggcaaacagg ctttcggaaa tgcgctgcgc ggcggcaagg gcttcttcgc cgtgaatcag
     4921 gcgggtcatt tcttcggcga ggatgcgttg cgcttcgggc ttgctgccgc ttgcctggtc
     4981 tttggcttcg acggcatcga tttcttcgat ggacaggaag gtaaagtatt tcaggaattt
     5041 atacacatcg gcatcggcga ctttcagcca gaattggtag aactgatagg gcgaggtttt
     5101 tttcgcgttc agccatactg cgccgccttc ggttttgccg aatttgatgc cgtctgattt
     5161 ggtaaccaaa ggcagggtca gtccgaacac ttgtttttgg tacaggcggc gggtcaggtc
     5221 gataccggca gtgatgttac cccattggtc ggagccgccg atttccaaaa ccgcgccgtg
     5281 gcgtttgttc aactcggcga agtcgtaacc ttgcagcagg gaataggcga actcggtgaa
     5341 ggaaatgcct gcgccgtcgc ggtcgatgcg ctgtttgacg gattctttgt tcagcatggc
     5401 gttgacggag aaatgcttgc cgatgtcgcg caggaagtcg aggcagttca ttttgccgaa
     5461 ccagtcggcg ttgttcgcca taatggcggc gtttccgcct tcaaagctca agaaaggggt
     5521 taattggctg cggatgcttt ccacccagcc ggcaacggtt tcggcggaat tcaagctgcg
     5581 ttcggcggct ttgaagctgg ggtcgccgat cataccggtc gcaccgccca ccagtgcaat
     5641 cggcgtatgc cccgcctgtt ggaagcggcg caatgccaat acgggcagca ggtgtccgat
     5701 gtgcaggctg tcggcggtcg ggtcgaagcc gcaataaagg gaaatttttt gttcgttcaa
     5761 caaagcgtct aaggcttcga tgtcggtggt ttgcgcgatt aggccgcgcg attgcaggtc
     5821 ttggatgacg ctcatcggtc tctttcaaaa aaaattagcg gttttgcaaa ccgccgattg
     5881 taacaaattt aagcgaatca atggttatgg cgcgtatcga gaaaccgttg tttttcggaa
     5941 aaacgcttcg ccaattccgt gccgccgtaa gggttgatgt ggtctttgtc cgagtaaacc
     6001 ggcaatccgc cgatttggaa atttgcgggg atataggcgg cggcatcaat aatatagacg
     6061 ttggggtatt tggctgccaa ttccctgatg cgtgcattgg ctttcagagt gctttcgtcg
     6121 tccgggcgca gggtttggcg gtaacccggt atgcgtgaag acaagatata ggcgcgttgt
     6181 acgttgtaag acgaggcaag gttgtccgcc atcaggtaaa cggcttgttt ttcggacgag
     6241 agtttatgca gcatacggtc gaattttttg aaaaaaccgg catcgtaggc aagggagcgg
     6301 ctgttttcgg gcatttggct gccccaccgc atcgccaaaa ccacttttga ataccggggc
     6361 aggtgttctt cggcatagcg gtaaacggcg cggcaggcgg cccagttttg gaatacgcgg
     6421 gacgcgtagc cttccacata ggcgcaggcg tcggcggaaa ccatagtggc agcccatttt
     6481 tcttttttgc ccacggcatc gaagaatgtt ttgtaatggt cggcgtggga gtcgcccaaa
     6541 accagcagtt ccggctgttt ttccgtatcc ccccataggc attgtttgcc ggtattgttg
     6601 tggcaggagg tgttggaacg cgtcagcccc aagcggtcgt attgcgccat aaacggcagc
     6661 ctcatcgcaa aaaatgagcc cgcccccaaa atgagcatag gcaaggcata aatccataaa
     6721 acggattgtg cgaacgaacc ttgccatttt ttaaacggtt tttcgatgca gtggtaagaa
     6781 aacagggaaa gcagcaatat caggacgacc gccgccgccg gcgaataagg cggcaggttg
     6841 tccgggccga tatagcgcat aaaggccaat atcggccaat gccacagata aagcgaatag
     6901 gaaatcaaac cggcggcaac ggtgattttc cattggaaaa attttttaag cgggtgttcg
     6961 taatgattga aataaatcag cgcggcaaca gccagacagg gaatcaaagc ggcgggccct
     7021 gggaaatagg cggtttgttc cgaataggca aacaggcagg cggacagtat gcacgcggaa
     7081 aacaatgcgc cggcggcggc acagcatctg ccgacggcga gattccgctg ttgccggtac
     7141 cgcatccaca cggcggtcag cgatccgacc agcatttcgc aggcgcgcag gtggggcagg
     7201 taatatttat cgaacgcgga aggcataaag gaagcggcaa ggcttaaggc gcacaatgcg
     7261 gcgaggaagc cgaactgtac gcgcgggctt ttgcgggcga caagcagcag caatatcgga
     7321 aagacaaagt aaaattgttc ttcgaccgac aaagaccaaa tgtgcagcaa gggcttttct
     7381 tcctgcgcgg gatcgaaata atccttcccc cttgcaaaat acaggttgga ggcgaaaccc
     7441 aaggcggtca gcgcggattt ccacaaaaga aagaaatcat ctttggtgaa taaaaagaag
     7501 ccgcctgcca gcgttgccgc caatacggcg aaaaatgcgg gcaaaatccg tttgatgcgg
     7561 cggatataaa atgccttcag ggaaaacccc cccccccccc cgacatttcg cgaaggagga
     7621 tcgccgtcat caaaaagcct gaaatcacaa agaatatatc gacaccgaga aatccgcccg
     7681 gcagccaatt tttttcgata tggaacacga tgacggacaa aacggcggcg gcgcgcaatg
     7741 tgtcgatgtc cgggcggtag ggtaaggctt ggctcataat gtttttatag tggattaaat
     7801 ttaaaccagt acggcgttgc ctcgccttgc cgtactatct gtactgtctg cggtttcgtc
     7861 gccttgtcct gatttaaagt taatccacta tactcgaaac gcggcggcgc aaatgccgtc
     7921 tgaaaggtca ttttgtacgg gatattcgga atgccggacg gctttttgca acagcggggc
     7981 aggcggtttg ttttgcagga atcggggagg gcaaatcgga aatgcgggtg ggattttatt
     8041 ttgatgcagc cgcattccgg cggtatggaa acgccgaaaa ccaccaaaac cggcttcaga
     8101 cggcatttct ggcaaagccg tctgaaaacc tgccgcattt gggttacacg ttaaacaaaa
     8161 agtgcatcac atccccatcc tgcacgacgt attctttgcc ttccacacgc attttgccgg
     8221 cttctttggc tttggcttcg ccgccgagcg agacaaaatc atcgtaggca atcacttggg
     8281 cgcggatgaa gccgcgttca aaatccgtat gaatcacgcc ggcggcttgc ggggcggtgt
     8341 cgcctttgtg tatcgtccac gcgcggactt ctttcacgcc ggcggtgaag taggtttgca
     8401 ggcccaagag gtcgtagccg gcgcggatca ggcggttcag gccgggttct tccaaaccca
     8461 tttcggcgag gaattcggct ttttcgtcgt cttccaattc ggcgatttcg ctttccatcg
     8521 cggcgcatac ggcgacgacg ggggcgtttt cttttgccgc caattctttc aggcggtcga
     8581 ggtgcggatt gttttcaaaa ccgtcttcgg cgacgttgcc cacatacatc gcaggtttgg
     8641 ctgtcagcag gaacagcggt ttgagcatcg cgcgttcttc cgcgtccaaa ccgaaggaac
     8701 gcacgggttt gccttcgtcc agatgcggca gcagtttttt gcacaaatcg accagctttt
     8761 gcgcgtcttt gtcgcctgaa cgggcgcgtt tttcttcgcg gacgatggct ttttccacac
     8821 ttgccaggtc ggcaagtgcc aactctgtgc cgatggtttc gatgtcggca atcggatcga
     8881 cgcggcctgc aacgtggacg atgttgtcgt cgtcaaagca gcgcacgaca ttcacaatcg
     8941 catcggtttc gcggatgttg gcaaggaact ggttgcccaa gccctcgcct ttgctcgcgc
     9001 ctgcaaccaa accggcaata tcgacaaatt cgacgatggc aggctgcatt ttttgcggat
     9061 tgacgatttt tgccaattcg gccatacgcg gatcggggac ttcgacgatg ccgacgttgg
     9121 gttcgatggt acagaaagga tagtttgccg cttcgatacc cgattgggtc agcgcgttaa
     9181 aaagggtgga tttgccgacg ttgggcaaac cgacgatgcc gcatttcaaa ctcatgtttt
     9241 ttcctgaaaa tagagaaatt taacggcgga ttatagcata ccgccgcccg cgttccgaaa
     9301 aaatgccgtc tgaaacggct tcagacggca tctggtttca aaaaccgttc agaacaagcc
     9361 gtgaatcacg ccttctgcgt ccacgtcgat tttctcggcg gcgggaactt tgggcagacc
     9421 cggcattttc atcatattgc cgcacaacgc aacgatgaaa ccagcaccgg aagaaacggt
     9481 aatgccgcgc acggtaatgc ggaagccttc agggcagccc aagagtttgg cgttgtcgct
     9541 caaagaatat tgggttttcg ccatgcaaat cggcattttg tccaagccca gtttttccag
     9601 cgaggcgatt tcggcagacg cttccgcgct gaaatcaaca tcttccgcgc cgtacacttt
     9661 ttgggcaatc gcacggattt tgtctttaat actcagctca acatcgtagg aaaaattgaa
     9721 gttgttcggt tggttttcga tggcgttgac gactttgcgc gccaagtctg caccgcccac
     9781 gccgcctttg ccccacactt cggtcaagga aacttcaacg ccgtgttcgg cacaggcttt
     9841 ttcaatcatc gccaactcgg catcggagtc ggacacgaag cggttgagcg caacgacgac
     9901 gggcagtccg aatacgtttt tcaggttgga aatgtgtttc agcaggttgg gcaaaccttt
     9961 tgccaaggct tcgaggtttt cttcaccgag gttggcacgt tccacgccgc cgttgtattt
    10021 caaagcgcgg acagtcgcca cgacgaccgc cgcatcaggt ttcaaaccgg caaggcggca
    10081 tttgatgtcg cagaattttt ccgcgcccaa atccgcgccg aagcctgctt cggttacggc
    10141 gtaatcggca aggtgtttcg ccagacgggt tgcggttacg gagttgcagc cgtgggcgat
    10201 gttggcgaac gggccgccgt gtacgaaggc gggcgtgcct tcgatggttt gcaccaaatt
    10261 gggcttaatc gcatctttga gcaggaccgc catcgcgccg tgtgccttca aatctttggc
    10321 gtaaacgggg ctgccgtctt tggcgtaggc gacaaggatg ttgcccaaac gctctttcaa
    10381 atcgctgatg tctttggcaa ggcagaatac cgccatcact tcggaagcaa cggtaatgtc
    10441 gaaaccgtca ggacgcatca cgccgtcaac aggtttaccc atgccgtcga tgatgttgcg
    10501 caactggcgg tcgttcatat cgaccacgcg ccgccacagc acgcgtttcg gatcgatgtt
    10561 caactcgttg ccttggtaga tatggttatc gagcatggcg gcgagcaggt tattcgccgc
    10621 accgatggcg tggaagtcgc cggtaaagtg cagattgatg tcttccatcg gcaaaacttg
    10681 ggcatagccg ccgcctgccg cgccgccttt cacgccgaac accggcccca gagaaggttc
    10741 gcgcagggca atcacggcat ctttgccgat gtggcgcaac gcgtccgcca aaccgatggt
    10801 tacggtggtt ttgccttcgc ccgccggagt cgggttgatg gcggtaacca aaatcagcct
    10861 gccctgtttt tgcggcagtt tgaacgcttc ggcaggattg attttcgcct tgtaatgacc
    10921 gtaaggctca atgttgtcaa cgttcagacc cagcttggcg gcaatttcgc caatcgggcg
    10981 catcgtggag gattgggcga tttcggcatc ggttttgaag ctcatgattt tcctttagaa
    11041 atgaggaggg acataccgtc tgaaagcatc aggcgacaaa caggtggatt gaaaataata
    11101 tcaggcatat tataacgtta tccgcaccaa acccgcagtg aaatttttga cgcagcaaca
    11161 aaaataccgt tcatattgtt cacaatccaa ggagaaaaca tgagcagcaa cgcatggctg
    11221 ttttgggcat tggcatcggc aggcttcgcc tcattgaccg ccatcttcgc caaaatgggt
    11281 ttacagggta tagattcaga tttcgctacc ttcatccgca ccttggtcat cctcgccgct
    11341 ttgttattgt ttttgaccta caccggcaaa tggcagggcg tgaacggatt tacaggaaaa
    11401 aactggacat tcttaattct gtcgggtttg gcgaccggcg catcctggct cgcctacttc
    11461 aaagccctgc aactgggcaa cgcctcgcaa gtcgcgcccg tcgacaaatt cagcctggtc
    11521 ctggtcgccc tgatggcggt ggtctttttg gacgaacgcc cgaacacgca ggaatggata
    11581 ggcttgggac tggtaacggc gggcgtgttg gtgctggcgt tgaaacgtta aaccgaatcc
    11641 gccataccgc ctgaaaccgg gttttgacaa tctgacgggc ggcggtctga acaaagttac
    11701 ccaataaaat ccgacaccgc gccgacggca aaccgccgcg cttcagatgg catttgttgg
    11761 acggaaagcc ccgaacgccc tattttcacg cctccacgaa aatatgccgg acatccggtc
    11821 ggaaccgttc ggcagccggc tcggcaagcc accgttttat ttcacttcca agcccctgca
    11881 agggcttgag cctctttcag acggcatacc gtgccgacat ccagccacaa gcccgtatgc
    11941 ttctgaccgc tcacgcggtt ttgccgcatt tcgccacgca atacgggcgc gagtttcgcc
    12001 acactgcccg cttcgattcc gtcaaacatt tcaggacggt aaatacccac gccgctgaat
    12061 gtcaatccgt tgccgccatt tacttccggc cgcacgctgc cgtcgggcag cagggaaaaa
    12121 tcgccgtcgg ggttgtgcgg cggattttcc accagccaca gatgggcgga aatatgttcc
    12181 ggcagggacg atgccgcctg aaacgcggcg gtaaaatcga tgtcggtcag cacgtcgccg
    12241 ttgaccacca aaaacggctg cccacccaac agcggcaatg cctgcgcgat gccgcctgcc
    12301 gtttccaaac cgcctgcggg ttcgggcgaa taggcgatgt tcacgccata agccgagccg
    12361 tcgcccaaag catcttctat ctgccggccc agccaagcgt ggttgatgac gatttcggta
    12421 aaccccgcct gcttcagacg gcataggtgc caaccgatta gaggcttacc cgccacatcg
    12481 agcagcggct tcggagtggt atcggtcaaa gggcgcatac gctcgccgcg tcctgccgcc
    12541 agtatcatcg ctttcatata tctgtccgaa tatcagtcta aaaatctaaa ctgccgtctg
    12601 aaatacagca gcgcggggcg tttgcacccg cagtttttga tttcgtcgag cctgacgtaa
    12661 aacacaaaat gcgtgccgat ttcatgtttg ccgacaatat gcccgtgcag gtgcgccaac
    12721 gcgccctcta tttcaagttg tcccgttttg ccgcgatgcc agatgtggta ggcaaaccgc
    12781 tcttcgggcg acagcccggt cagcccggca aaatgttcgg caacatcctg atgttcgtcc
    12841 gccagcgtat tgatgcagag gctgccgttt tccgacagga tcggaatgat tcgcgcactc
    12901 cggttgatgc acagcatcac ggtcggcggc tcgtccgtaa ccggtgtaac cgccgtcatt
    12961 gtaatgccgt aacgccctgc cgcaccgtct gtcgtgatga catgaacgcc tgccgcacaa
    13021 gatgccatcg catcacggaa cgaagtttga aaagttttct gcaaatccgc catttttccc
    13081 ctttaaactg tcccctatat aagaatgctg cacacaaggc accccccatg tgcagcagtt
    13141 ttgattcaaa aagccgtcgg tcggacgttt ccgcgcgtta cggcgtatta cgagttcaac
    13201 gcatcctcga ttttggcaag ttctgccaac aggtctttaa gcagcagcat tttctcgcgg
    13261 cccagcactt cctcgatagc gtcgtaacgc tcgtccactt cttcgccgat ttcctcatac
    13321 agcttctcgc cctcggcagt cagcttcaga aaaacgcggc gttggtcgtt ggaaggtttc
    13381 aggcggacaa ccaaacccgc tttttcaagg cgggtcagga taccggtcag gctggggcgc
    13441 agaatacacg cctgattcgc caaatcttga aagtccagcg tgccgttttc cgccaaaaga
    13501 cggataatcc gccattgctg atcggtaata ttcgcctgat tcagaatagg cctgaattgg
    13561 gtcatcaggg cttcccttgc ctgtatcaga ctgatattga tagacgcatg ttttgattgg
    13621 gtaggcattg ttcaagtctc caagttatcg aaaatcaaac tttcaaaccg tcgggaaagc
    13681 ctgtgggcgt aaattttgat gcaaccgtta tataacaaaa cgaacatata gcaacaatac
    13741 gctataaacc gcatcggacg actgggtata aaagacttta attccgataa tcctatctaa
    13801 aaatatttta atagttatat cttagtctat tttccctaca atctcaacaa agtattacat
    13861 ttactgtcga aagaactttc ttccagaaaa aacaaaagcc gccgcatcag acgagcaagg
    13921 tatatgccgt ttgattctct acatagcgta aagtttaaca aaaacaaaag ttaaccgaaa
    13981 acatccgcct gaaaaattcg tgcgcgcaag ccccaataac tgctgattcc cgtcgtatag
    14041 tgaaccattt tcccattttt gaccaaaacg acggcaggcg ttgcgacaat ccgccaagac
    14101 cttgccaaac ccccgtcctc atcgttgacc gtcggaaagc ccaagccccg ctttgccata
    14161 tacgccgcca cttcggacgc gctgccggaa cgtaccgcca cgccgacgac cggcacgccg
    14221 tccgccgcca aatcatcgat tatcggcgac tgataacggc acacgccgca ccagctcccc
    14281 caaaaataca ccaaaaccgc cttatcccgg ctaaactgtc ccaaagtcag ccgctgcccc
    14341 gacagcaggg tcaaaggccg ccctgccgca ccggccggct cttcgggctt gcgtacccaa
    14401 tccaaaaaca gcgacaccaa taaaaacacc aatgccgtct gaacggcaaa tttgatgccc
    14461 gaaagcagtt tctttttcat acgctctctc aaacggtacg cccgcgcaac cggcaggcaa
    14521 acaaaaagcc aagtctcaaa acttggcttc cggttatctg gtgggtcgtg agcgattcga
    14581 acgctcgacc aacggattaa aagtccgctg ctctaccgac tgagctaacg acccgataag
    14641 ccgtgcatta tacagcacca tcctacctcg tcaagcaaat tttacaggct taattgcaga
    14701 ccactgtttg cacgggatat tttgacaacg gattttcaca atccgccgca taccgtgtaa
    14761 aagttcgcac aaggaaaagc aaaccgcccg aaatcaatgt acactttccg cccgtttccc
    14821 aaactgcaca cagaaacaca cattatgaac atacaaaaca tccgcaccct cctcgacacc
    14881 gtcgccgttc cgaatacggc acgcacgctc ggcggcgaaa aggccgtccg ttcggtcgaa
    14941 cagcattcag acggcatcca tatcgccctg catttcggct tccctgtcgc gcacatcgcc
    15001 gccgccatcg ccgattccgt acaagaaacc ctgatgcccg aaacaggcga cacacacatc
    15061 catctgtcca tggacactga aatcggcaca cacaaagtcc agcccggcgt taccaccatc
    15121 aaaggcgtga aaaacatcat cgccgtcgca tcgggaaaag gcggcgtggg caaatcaaca
    15181 accaccgcca accttgccgc cgcaatggcg cgcatgggcg cgcgcgtcgg cgtgctcgat
    15241 gccgaccttt acggcccgag ccaaccgacc atgttgggtg tggacgaccg caaacccgat
    15301 cagaaaaacc aaaaactcat tcccgtcgaa tcttcaggcg gcatacaggt tatgtccatc
    15361 ggctttctag tcgataccgg ccaagccgtc gtctggcgcg ggccgatggt cagccaagcc
    15421 ttgcagcaac tgatgttcca aagcgagtgg gacgaagtgg actatctttt catcgacctg
    15481 ccccccggca cgggcgacat ccaactcacg ctgtcccagc gcatccccgt taccgggtcc
    15541 gtcatcgtaa ccacgccgca ggacatcgcc ctgatagacg cgcgcaaagc cgtggatatg
    15601 ttccgcaaag tcaacattcc cattttgggc gtattggaaa atatgtccgt ccacatctgc
    15661 tccaactgcg ggcatagcga agcactgttc ggcacggacg gcggcaaaga tttggcggca
    15721 cgcctcaacg tccccctgct cggacagctt cccctaagcc tgcccgtgcg cgaagccatg
    15781 gacggagggg cggcggcaca actgttcgac aagcaccccg ccatcgcccg aatctacacc
    15841 gatgccgcat tccaaatcgc cctgagcatt gccgacaaag gcaaagactt cagcagccgg
    15901 ttccctaaaa ttgtcgtcga ataaagccgc gtccgaaacc gcaacagcaa tgccgtccca
    15961 agccccgcgc ctgccgatat gccgtcattc ccgcgcaggc gggaatccag gtctgtcggc
    16021 gcagaaactt atcggataaa acggtttctt gagattttac gttctagatt cccactttcg
    16081 tgggaatgac ggaatgtagg tttgcgggaa tgacggttta ggtattttta tagcaagccg
    16141 tagggtggga tgccgccgga gggcatcata ttcttggaat agcgcattaa aaagagtatg
    16201 ataatggaaa cacaaaagat tccggcaatg cccgtgcgcc gttcccgtcc gtcattcccg
    16261 cgcaggcggg aatccagacc tgtcggaacg gaaacttatc ggataaacgg tttctctaga
    16321 ttccgcatcc t
//
LOCUS       DAWFAN010000032        15317 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWFAN010000032 DAWFAN010000000
VERSION     DAWFAN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.032"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            465..1244
                     /locus_tag="WGO65_000723"
     CDS             465..1244
                     /locus_tag="WGO65_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0327045.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(1455..2867)
                     /locus_tag="WGO65_000724"
     CDS             complement(1455..2867)
                     /locus_tag="WGO65_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0327046.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLIAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEEWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGAINYIGTFERVYSEVSALLDSGKASFDWCNTEAVRFFP
                     ALGSLHAYKIKAELVRHYGADCYVSAKNLLKTFTTVRFEKGSDGMLDAKTAALKNGVA
                     DNPPTGLFVNKKAARRALSLWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            2958..3034
                     /locus_tag="WGO65_000725"
     tRNA            2958..3034
                     /locus_tag="WGO65_000725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2992..2994,aa:Arg,seq:acg)
     gene            3052..3126
                     /locus_tag="WGO65_000726"
     tRNA            3052..3126
                     /locus_tag="WGO65_000726"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3085..3087,aa:Glu,seq:ttc)
     gene            3724..4899
                     /locus_tag="WGO65_000727"
     CDS             3724..4899
                     /locus_tag="WGO65_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ0327047.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKYVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATVGR"
     gene            complement(4959..5507)
                     /locus_tag="WGO65_000728"
     CDS             complement(4959..5507)
                     /locus_tag="WGO65_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ0327048.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(5510..7006)
                     /gene="ccoG"
                     /locus_tag="WGO65_000729"
     CDS             complement(5510..7006)
                     /gene="ccoG"
                     /locus_tag="WGO65_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ0327049.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            7534..9513
                     /gene="tkt"
                     /locus_tag="WGO65_000730"
     CDS             7534..9513
                     /gene="tkt"
                     /locus_tag="WGO65_000730"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ0327050.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEAEWNELFAQYQAKYPTEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKALPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAIKAAD
                     HPSCLIFSRQNLKFQARNEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     VLAEQGIAVRVVSMPSTNVFDRQDAAYQTAVLPEGLPRISVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            9654..11042
                     /gene="fumC"
                     /locus_tag="WGO65_000731"
     CDS             9654..11042
                     /gene="fumC"
                     /locus_tag="WGO65_000731"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ0327051.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(11107..12030)
                     /locus_tag="WGO65_000732"
     CDS             complement(11107..12030)
                     /locus_tag="WGO65_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0327052.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALIVVGGAVTAAVGDRLFKRR"
     gene            12202..12342
                     /locus_tag="WGO65_000733"
     CDS             12202..12342
                     /locus_tag="WGO65_000733"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327053.1"
                     /translation="MPSENSGDVRQASLPQNARTAQSQRSAQRKGRQQAGSDAKKRNP
                     AP"
     gene            complement(12402..12926)
                     /locus_tag="WGO65_000734"
     CDS             complement(12402..12926)
                     /locus_tag="WGO65_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ0327054.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            complement(12929..14311)
                     /locus_tag="WGO65_000735"
     CDS             complement(12929..14311)
                     /locus_tag="WGO65_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0327055.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            complement(14422..15045)
                     /locus_tag="WGO65_000736"
     CDS             complement(14422..15045)
                     /locus_tag="WGO65_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0327056.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
BASE COUNT         3888 a         4417 c         3625 g         3387 t
ORIGIN      
        1 tataacgaat tttaattggg attatgtaaa actgtatata atcaattcag atttccaaaa
       61 aattgttttt tattataaag gtaagatagt ttttgaggaa ttaatagaac tagatggaga
      121 agggaatgtt ttacctcaat atctatttga ttcagatttg gaaaatattg aatattacga
      181 atgtgattac aagaatggca agatgcggct tttaaaaaaa gaaaaatcac attttttgat
      241 ggatattttt attattataa acctataaat tgcaggccga aacttttgta aaaccgtcca
      301 tactcattac cgcctagtcg gtaatgcatt ttcttagtag tatttgaaaa agtatgatga
      361 taaaaacggt caagcctaac tttttcataa aatccagcag agcaaggcag aaaaaaacac
      421 ccaaattggc tataatcccg acaaacacac tcaaggacaa caacatggca gcctcgcccg
      481 aagcaaaatt cactgaagaa aagattttgt gggtcaaaca ccacacgccg aaactcatca
      541 ctttcgccat cagccgtccc gaatcctacc gttttaaagc cggacagttc tcccgactcg
      601 gtttctacga aggggaaggc tttatttggc gtgcctactc catcgtttcc gcagaatatg
      661 ccgacacgct cgaatatttt gccgtactca tccaagacgg ccccatgtcg gcccgtttcg
      721 ccaaaatgca acagggcgac accatcctgc tcgataaaaa tgccaccggc ttcctcctgc
      781 ccgaacgctt ccccgacggc aaggatttgg tgatgctctg caccggctcc ggcatcgccc
      841 ccttcctttc cattctcgaa caacccgaaa tccggcagcg tttcgatacc gtcaacctga
      901 tacattccgt atcttttccc gaagaattga ttttcaacga ccggctcgcc gcattgtccg
      961 aacatcccct ggtaggcgaa tacagacact cgttccattt cgtccctgtt accacccgtg
     1021 ccgccaaccc ctcgggctta agcggaaaac gcattccgga actcttaaaa aacagcagca
     1081 tcgaacaggc gctgcatacc aagttcaccc cggaatccac acggtttatg atttgcggca
     1141 acccggaaat ggtcaaagat actttccaaa cgctgcttga catgggttac gccatgcacc
     1201 gcaaccgcat tcccggtcaa atcatgatgg aaaacggctt ctaaaaacca ccctgcttgt
     1261 ccgatgcctt cggatggacg gtcaaaccga cacggcacga aaaccgcgtc ggcaaaaatg
     1321 ccgtctgaaa aaattcagac ggcatcttcg gacacattac ctgcaaacgg caacacattc
     1381 caacccaaac cgattaggca atcaatacga acggctgttt acatacttac cggctttcat
     1441 caaccggtat cgatttaacc gatttcctta atatttttcc tgtccgtttt aaacttcgcc
     1501 ttaaacgcat ccggtaaatc tttatcgaaa taccaaagcc cgtcacccat ttccaatgcg
     1561 ccgcccattc catgcagaac aactttttcc cccgttaaag gatcagtttc ggtaatttct
     1621 acctcatgca tttttcccca atccaaaata ggcaacttgt gtgcaaacgc caaaacctgt
     1681 tcgtcagaac ggccgcacgc cttatcgcat cggcctgaaa catatacggg acaaggctca
     1741 tcctctgcat aaccgtccgg aaggataccg gctgcggcag gacacaatcc gtatgtttcc
     1801 gcccacaacg acaaagcccg tctcgctgcc tttttattga caaataatcc ggtaggcgga
     1861 ttgtccgcca caccgttttt caaagccgct gttttcgcat ccaacatgcc gtctgaacct
     1921 ttttcaaacc tgacggtcgt aaatgtttta agcagatttt tggcagatac gtaacaatcc
     1981 gccccataat gcctgaccaa ttccgcttta atcttatatg catgcaggct gcccaatgcg
     2041 ggaaaaaaac ggactgcctc cgtattgcac caatcaaacg aggcttttcc ggaatccaat
     2101 aaagccgaaa cctcgctata tacccgttca aacgtaccga tataatttat tgctcccccg
     2161 ccgtcgaaac aggcaagcac ccccataccg tcaggcaaac cgtacaactg ttccctcaac
     2221 cgttcgggca gcgcggcagg cagcggtttc ggattcatca aacggaaaca ctgcctgatc
     2281 cactcctcga ccccgtgttc cgacagactg tattccaaat aatcacacaa tgccgataca
     2341 tccgccatcg cacgatgcct gtcttccaca acaatcccca acctttcgat gatactgtcc
     2401 aggctgtgct tgtaaaattg cggatacaga caccgggaca gctgcacact gcacaaagca
     2461 ggcgatgaaa atccgatacc cgcacgatga aactcatgct ttaaaaacgt atagtcgaaa
     2521 cggctgttat gtgcaatcaa cacacaaccc ttcaataccg aaaacaactc gccggcaatc
     2581 tctgcaaaaa caggtgcgtc ggcaaccatg ccgtctgaaa tccccgtcag ccccgccaca
     2641 aactgcggaa taggtttttg aggattaacc agccactcat gccttaccac ccttccctgc
     2701 tcaaacttga ccaaagccac ttcggttacc ctgtcttcat acagattgcc gcccgtcgat
     2761 tccaaatcaa ccacggcaac aggcattcca aaccgtaaaa ataccttttc cagcaagggc
     2821 cagcgggaag caacaatcat tttattctct ttaaattcaa acaacaaacc gatattttac
     2881 acttttaagg catttcatcc aacaaaacaa ttgacagaat ccgatgatta ccctaaaatt
     2941 caaatctttc ttgcagcgca cccgtagctc agttggatag agtatctggc tacgaaccag
     3001 agggtcgggc gttcgaatcg ctccgggtgc gccagtaaga aaatacaata tgcgcccatc
     3061 gtctagcggt taggacatcg ccctttcacg gcggtaaccg gggttcgatt ccccgtgggc
     3121 gtgccaaatt ccaaatcccc gagattatcc ctcggggatt ttttattgtc tcggcaactc
     3181 gttaccatat ctttacctac ccccttcatc agaatctcag acacaatcga atcatattca
     3241 aacctttgcc gtgcaaaccg atatcccata accggatgcg gtgtccgtcc aacattttac
     3301 ccgattgaaa cgcctgatat atcgcacccc atcaaagggg cattgctttt cttaacaatc
     3361 ccctttgaca gggctttttt atgccatcat caaatttata gtggattaac tttaaaccag
     3421 tacggcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg gagcaccaag
     3481 tgaatcggct ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
     3541 taattcacta taatattttc tctcccgatt gaaacaggcg taacagaata tgcccgaagc
     3601 tccggctgct ttcttgttta ccgccgcgat atttagagta taataccaaa tttgagcaat
     3661 agttctaaaa cagttagaac catttttcat gagcctgact gattcgtaca ctcggagaaa
     3721 ctgatgcaga atatttttga ccctttggtt attcgtggaa aatcccttac ccccatcgtg
     3781 caaggcggta tgggggtcgg tgtttccgca tcgggtttat ccagcgcggt ggcacgtgaa
     3841 aacggtatcg gaacgattgc cagtgtggat ttgcgccacc ttcacgaaga cctactcgcc
     3901 gaatcccaaa tcaatccgag tgaagagaaa tatacgtctt tgaactgtac cgcattggac
     3961 agggaaatcc aaaaagccaa aagtgcttca gagggaaaag ggctgattgc ggtcaacgtg
     4021 atgaaggcgg tcaaagacca cgccgcatat gtccgccagg cttgcgaatc aggggcggat
     4081 gcggttgtaa tgggtgccgg tctgccttta gacctgccgg aaatgaccga gggctatcat
     4141 aaatatgtcg cgctgctgcc gattctgtcc gaatcgcgcg gtattaatat cgttttgaaa
     4201 cgttggatga aaaaaggcat attgcccgat gcgattgtag tcgaacatcc tgcccacgcg
     4261 gccggacatt tgggtgcatc aaccgttgaa ggcgtaaacg atgccaagtt cgacttcaaa
     4321 cgcgtgattg aggaaacgtt tgaagttttc aaaagtttag ggctggaaag cgaaaaaatc
     4381 ccgcttattc ttgcgggagg catggcaaat tttgaaaaag tcaaaaccgc cctaaagaac
     4441 tggggagcat ccgccgttca aatcggtacg gcttttgccg ttaccgaaga aggagatgca
     4501 caccttaact tcaaaaaaac gctcgccggt gcggaaactg aaaaagtagt cgaatttatg
     4561 tctgttgccg gtttgccggc gcgcggtgtc cgcaccaaat tcctagacag ctacatcaag
     4621 cgtgaaagca aacttcagac aaacgccaaa gccgacccgc gccgctgtac ccaaggttta
     4681 aactgcctaa ccagttgcgg tctgcgcgac gggctttcca aagccggaca attctgcatt
     4741 gatatccagc ttgccgccgc attccgtgga gaagtagata aaggcctgtt cttcagaggt
     4801 aaagacccgc tgcccttcgg caatgccatc cgcaccgtcc gcgagacgat acaatatctg
     4861 ctgacgggga gcgaacctgt tgcaacagta ggacgctgat gttaaaacag ccgataccgt
     4921 agcaaaatgc cgtctgaaaa ttttcagacg gcatctcttt atttgctttc agtattattg
     4981 aaaagtttgt ccatcggggt caaatcgacc gcattgcctt ggctggtaat ccatttgttc
     5041 tcgacgcgcc acacgcctgc cgcgtcctcc acgcgcacat accagtggtt ggtcggcgaa
     5101 agggttttga acaccgcctc atattccgcc ctgccgttct gcgcgctgcc gacgggcttg
     5161 agggcgacgg tttgatcgtc cgccttgcgg gtcgggtgca tcagcagcag gttcaaaggc
     5221 tgtttgccgt caaactcgcc gccgacaaac acttttgccg cattcatatc aggggaaatg
     5281 agaacctgca ccccgatatg ccgtctgacg gcttcttcat cccgatgaag ctggatgtcg
     5341 atatgcttgc cgtctttata ataatcgtcc gtaaccaaat ctgtcgcgtg ctgctgcgcg
     5401 acaaaaaaca tagcgacgct ggcgatgacg acaaaaatcg gccccgccat caagatccac
     5461 ggccagacgt gtttgtacca aggtttgatt ggagtgtttt gagacacggt tattctccga
     5521 taaaggttgc atcttcttcc aagacgaccg gcttgccgtc gggcgcgccg ctttcgcggt
     5581 attggaaggt aaattcgata gggtggctgc ctttgtccgc gtattccgga atggtggaca
     5641 cttggacggg aagggttacc gtttcgcgcg gggcaacctt gataccgcct tcgggcagcc
     5701 cggtcagggc gatttcgtca aagcctttga cacttgcggt aatgagctgt tctttttcac
     5761 ttttgttgat gatacgcagg ctgtatgcgt tttccagcca gcctttggcg ttttcgcgca
     5821 ccagtacgcc acggtctttc aaaatatcga cctcgaccat tttgcgcgtg gacaaaccgg
     5881 ccaggaaggc aatgataact aacgccaaca ccgcgccgta acctgccacg cgcggtctga
     5941 gcagccgttt tttaatgtct ttttcagaat attcgtgttc cagcgcgctt tcggtcgtat
     6001 aacggattaa tccgcgcgga tagcccattt tgtccataat ctcatcgcac gcgtcgatac
     6061 aggcggcgca gccgatacat tggtattgca gaccgttgcg gatgtcgatg ccgacggggc
     6121 agacttggac gcacatcgca cagttgatgc agtcgcccaa acccgcctct tccttattga
     6181 ccgttttctt gcgcgcgccg cgcggttcgc cgcgttccgc gtcataagaa acaatcagcg
     6241 tgtccttgtc gaacatcgcg ctttggaaac gtgcatacgg acacatatgc aggcatactt
     6301 tttcgcgcat aatgtgggcg aagaagaagg tcataaagcc gtaaaacgct gtggcaaaca
     6361 tcgcgccgcc gcctgctgct ccagtgaata aatcgggaac gaactggcgg atagggacaa
     6421 accagcctgc aaacgtgatg cccgtccacg cgcagacaag gaaaatcagc aggtatttgg
     6481 tggctttgat gcggatttta gtgaaattcc acggcgattt ttccagtttc agccgtttgt
     6541 ttctatcgcc ttcgaccagg ttgtcgatcc acagcataat ttcggtgtaa accgtttgcg
     6601 ggcaggaata gccgcaccac agtcgcccgg caatcgtcgt ccaccaaaac agcccgaagg
     6661 cgcaaatcat cagcagcaag gcaaggtaaa tcaaatcgcc cacccccaac gacaatccga
     6721 aaatgaagaa atgccgttcg gggatattga aaacgacagc ctgcctgccg ctccagttga
     6781 accacggaat gacgtaaaac acaaactgcg tcgccaatac ggcggcgata cgcagtttgg
     6841 cgaaccgtcc ttccgccttt ttgggatgga tgcgttcgcc ttcgggatgg atttgaatca
     6901 cgctggctcg cggatcgaat gttttttttg ctttcggcgc ggcttttgtt tgttcggacg
     6961 tgccgattcc ggatgccgga ctgccggctt ggttttccgt ggtcattctg cattccttag
     7021 atttttgatt gatggtttgc ccgttaccgc cgccgtttgc ttttcagacg tcatttttct
     7081 tgttttttaa ggcgttgtgt ttcaagtttt gagaaaatcc gtttttccca aaatatattt
     7141 ccgctattgt acaactttat gcgccgtctg gatgtatggg gcggatacat ttcccatccg
     7201 catcaaaacg cctggatttt accttaccgc ccgaacaaaa tccgaatacg gttaaaaaaa
     7261 aagactaaaa aaaccgacac ccccatatcg gcagaaccga cggcgcaagc tcataaacaa
     7321 acgctatcga caatccggca cacaatctat aacattttat ttcaaaagga ataatggcag
     7381 gcttcgcccg caaatcgaaa atccttcccc accggtcccc tgccgccgcc ttcccccgcg
     7441 tccgcccttt tcttgaaagc ataagcgaat cgggcgataa tcaacgccgt ctgaccatcc
     7501 acttatccga aacaccagca aggaaaatac aaaatgtctc aactggcaaa cgcaatccgc
     7561 ttcctctccg cagatgccgt tcaaaaagca aattccggcc accccggcgc gcctatgggt
     7621 atggcggaaa tggcggaaac attgtggacg aaattcctca atcacaatcc cgccaacccc
     7681 aaattctaca accgcgaccg cttcgtcctc tccaacggcc acgcgtctat gctgttgtac
     7741 agcctgctgc acctgaccgg ctacaatctg agcattgaag acttgaaaaa cttccgccaa
     7801 ctgcacagca aaacccccgg ccatcccgaa tacggctata ccgacggcgt ggaaaccacg
     7861 accggcccgt tggggcaagg gattgccaac gcggtgggca tggcattagc cgaaaaaatc
     7921 cttgccgccg aatttaataa agatggtttg aacatcgtcg atcattacac ctacgtcttc
     7981 atgggcgacg gctgtctgat ggaaggcgta tcgcacgaag cctgttcgct cgccggcact
     8041 ttaggcttgg gcaaactgat tgttttgtat gacgataaca atatttccat tgatggtaaa
     8101 gtggacggct ggtttaccga aaacatcccg caacgctttg aaagctacgg ctggcacgtc
     8161 gttcctaacg taaacggtca tgacaccgcc gccattcaag ccgccatcga agccgcacgt
     8221 gccgaaaccg gcaaaccgtc catcatctgc tgcaaaacct taatcggcaa aggcagtgcc
     8281 aacaaagaag gcagccacaa aacccacggc gcacctttgg gcgcggacga aatcgaagcc
     8341 acgcgcaaac atttgggctg gacttacccc gcctttgaaa tcccgcaaga aatttacgat
     8401 gcgtggaatg ccaaagaaca aggcgcgaaa ctggaagccg aatggaacga actcttcgcg
     8461 caatatcaag ccaaatatcc taccgaagcc gcagaattcg tgcgccgtat ggataaaaaa
     8521 ctgccggaaa actttgacga gtacgttcag acggcattga aagaagtgtg cgccaaagcc
     8581 gaaaccatcg ccacccgcaa agccagccaa aacagtatcg aaattctcgc caaagcactg
     8641 cccgaactgg taggcggttc tgccgacctg accccgtcca atctgaccga ctggtcaaac
     8701 agtgtctccg ttacccgcga caaaggcggc aactacatcc actacggcgt gcgcgagttc
     8761 ggcatgggtg cgattatgaa cggtttggta ttgcacggcg gcgtaaaacc cttcggcgcg
     8821 actttcctga tgttcagcga atacgagcgc aatgccctgc gtatggctgc gttgatgaaa
     8881 atcaaccctg tatttgtgtt tacccacgac tccatcggtt tgggcgaaga cggcccgacc
     8941 catcaaccga ttgagcaaac cgccaccctg cgcctgattc cgaatatgga cgtatggcgg
     9001 ccgtgcgaca ccgccgaatc tctggtcgct tgggcagaag ccatcaaagc cgccgatcat
     9061 ccgtcctgcc tgattttcag ccgtcaaaac ctgaaattcc aagcgcgcaa cgaacaacaa
     9121 ctgaacgata tcaaacgcgg cggctacgtc atcagcgaag cccaaggcaa cgcccaagcc
     9181 gtcatcattg ccaccggttc cgaagtcgag ctggctttgg aagcgcaaaa agtattggca
     9241 gaacaaggca tcgccgtgcg tgtcgtttcc atgccgtcca ccaacgtatt cgaccgccaa
     9301 gacgctgcct atcaaaccgc cgtcctgccc gaaggtctgc cgcgcatctc cgtagaagcc
     9361 ggacacgccg acggctggta caaatacgtc ggactgaacg gcgcagtcgt cggcatcaac
     9421 cgcttcggcg aatccgcccc tgccgattta ctcttcaaag cattcggctt taccgtagac
     9481 aatgtggttg atacggtgaa atcagtgctg taaacccaca cctaaacaaa tgccgtctga
     9541 acgcttttca aacggcattt ttttgccctc ccgccttgat gcttcctcat tccgccaatc
     9601 tccattataa tatttgcgaa tcactcttat tcacatttca aaaggagaaa cgtatgaaca
     9661 cccgcaccga acacgacacg atgggcaatg tcgaagtccc gtccgaagcc tattggggcg
     9721 cgcagaccca gcgcagccgc aacaatttca aaatcggcgg cgaaaccctg ccgcagccgc
     9781 tgatttatgc tttggcgctg gtgaaaaaag ccgctgccgc gaccaatgtt tccctcggca
     9841 ggattaagcc ggaacaggcg gatttgatta cgcaggcggc ggatgatgtg ttgaacggca
     9901 ggcttgacgg acagtttcct ttagtggttt ggcagaccgg ctccggcacg cagtccaata
     9961 tgaacatgaa cgaagtgctg gcgaaccgcg ccaacgaaat tgcaggtacg ggtttggcgg
    10021 cttatcagcc cgtccatccc aacgaccatg tgaaccacgc gcaatcgacc aacgacgcat
    10081 tcccgaccgc tatccacgtt gccgccgcga ttgaaatcaa ccgccacctc atccccgccg
    10141 taaaagccct gcgcgacacg ttggacaaaa aagcccaagc tttcgcccct atcgtcaaaa
    10201 tcggccgcac ccacttgcaa gacgcgacgc cgctgacttt gggacaggaa ttttccggct
    10261 acgtttccca gcttgatcac ggtttaggcc gtctgaacga tgcgcttaaa gacttgtatg
    10321 aacttgcttt gggcggtacg gcggtcggca cgggtttgaa cagccatccc gaatacgccg
    10381 aaaaagccgc cgccaaactc gccgaactgt ccggcttgcc gtttgtcagc gcgccgaaca
    10441 aatttgaagc cttaggcgga cgcgatgccg ccgttgccgc ttcgggcgca ttgaaaacac
    10501 tggcggcaag cctgaacaaa atcgccaacg acatccgctg gctggcaagc ggcccgcgtt
    10561 gcggtttggg cgaaatcaaa atccccgaaa acgagccggg ttcgtccatc atgccgggca
    10621 aagtcaaccc gacccaatgc gaagcaatga cgatggtgtg ctgccaagtg ttcggcaacg
    10681 acgttaccat cggtatggcg ggcgcgtcgg gcaatttcga gctgaacgtc tatatgcccg
    10741 tcatcgccta caacctcttg caatccatcc gcctgttggg cgacgcgtgc aacagcttca
    10801 acgaacactg cgccgtcggc attgaacccg taccggaaaa aatcgactat ttcctgcacc
    10861 attccctgat gctcgttacc gcgttaaacc gcaaaatcgg ttacgaaaac gccgccaaag
    10921 tcgccaaaac cgcctacaaa aacaacaaat cgttgcgcga aaccgccgtt gagttgggct
    10981 tgctgacggg cgaagaattt gacgaactgg tcgttcctgc cgatatggtt catccgcgct
    11041 aatctttccc tcaaataaaa tgccgtctga aacctcgttc ggacggcatt ttccgttgcc
    11101 tgcgaactag cggcgtttga acagcctgtc ccccaccgcc gccgtaaccg cacccccgac
    11161 cacgatcagt gcgccaacat aacccaaacc gttcatatcc ggcgcggcaa aagtatcagg
    11221 catcacataa tgcccgagca aagaaaatat tacggtaaac acggggagca aggttgttac
    11281 cgcgctgact ttggaagcct cccaatgttt caacgcctcg ccgaacgagc cgtaaccgat
    11341 taacgtattc aagcagcaat acgcaaaaca aacccacgcc aacgtaccgt ccaaacttcc
    11401 gatgtgtgcc ggttcggcaa acggcaggaa cacggcggca cttgccgcat aaatcaacag
    11461 cagaatctgt tgcggcccga attgcgccga cagcagcttt tgcgccacgg cataacacac
    11521 ccatgccata ctgcctgccg cacacagcaa cacgcccttc gcatacgcgc ccaaacccga
    11581 caactcgccg aatttatcgt taaaaaacat aagcaaaccg gcaagcagca aaaccaagcc
    11641 gattttctga gcggcagtca tccggtcttt aaacaccaac acaccgacaa caatcatcgt
    11701 aaacggcgaa atctgccaca aaacctgcgt cgtggtcggc gaaatataat gcagcccttg
    11761 ggcaatcagc acaaagtttg ccgaaatgcc cgccacgccg agcagcagca gcctgaatga
    11821 gcaccaagaa aaatcccgcc acttcggcag ccgcccgccc aatgccagca aaacaaacaa
    11881 taccgccgcc gccacggtaa aacgcaccca caccagcgtc ggcgcatcga caaacttcaa
    11941 tacctgccgc acggcaatcg gcagcgttcc ccacgtcatc gccgccaaaa gtgccaacgc
    12001 gaagcctagg agcggccttt ggttttccat cctgattttc ctatttttaa acaaccgtat
    12061 tgccggacga tgccggtttg ccgcatcggg caatgatggt tcaagcgttt gacgtttgat
    12121 tccaaccctt tgatttcaaa caaaccggct gaagcccgac tattgcttcg cgctatttgg
    12181 aaacaccgcc tgaattttaa aatgccgtct gaaaacagcg gggacgtgcg gcaggcatca
    12241 ctgcctcaaa acgcccgaac ggcgcaatcg caacgttccg cccaacgcaa ggggcggcaa
    12301 caagccggct cggatgcaaa aaagagaaac cctgccccgt aaggtttaag gtttctccgt
    12361 cctttatgat ttccctctgc gaggatgtcc ggccgtaaaa ttcagaacgg gatgtcgtcg
    12421 tcaatatcct cgaccggggc ggcggcaggc acgggttggc ggcgcggcgc ggctggtgct
    12481 tcttggggat gggacggcgc gtcggaggcg ggctgccggc tttgctgtgc ggggcgttgg
    12541 taagcctcct gactctgacc gtaaccttcc tcgtaaggcg caccgccgct gttttcattg
    12601 cgcccgccca acattttcat ttcgttggcg acaatatcgt aagcggtgcg ttcgatgccg
    12661 tctttgcctt ggtatttgcg gctttggatt ctgccttcca gataaaccag cccgcctttt
    12721 ttgaggtatt gcccggcaat ttccgccagt ttgcggtaca tcgtgatgtt gtgccactca
    12781 gtacgctcta cacgttggcc gttgcggtcg ttccaagttt cgctggtggc gacgctgaaa
    12841 ttacacaccg cctcgccgtt gggcatatag cgcacttcgg gatcgcgtcc gaggcggccg
    12901 ataaggataa ctttgttcaa tgacattttt aaactcctgt aatgattttt tcagcggcag
    12961 cctgatcgaa acccttctgc aacactttga gatagacggt ctgcccgtcg aaactgaaac
    13021 cgatgtcttc cacgccttcg agttgcaaca gggcgcgttg taagccgtcc tgattgacct
    13081 gccacacgcc gccgacaggg taactgaggt ttttgacggg cttgggcgca ggcgataaaa
    13141 cggcaatcac cagccacagc agcatcaata tgctgcaaaa ggcaaacacg ccggaaaagc
    13201 cgtatttttg aaacagcaaa ccgcctgcca cgccgccggc aaacagtccg agcgactgca
    13261 tcgtgttgta cacgcccatc gccgtaccct tcaggtcgga cggcgcgatt ttggaaacca
    13321 tagacggcag gctcgcttcc aacacattaa aaccgataaa gtaaacaacc aaataagcgg
    13381 taatcaagcc taccgagcgc ataccggaca gcaaaccgag ctgcgccgcc gcgatgcaga
    13441 cgatgcccaa aacaaaaacc tgtttgagct tgttgcgcgt ctctccgacg ataatcagtg
    13501 gaaccatcac caccaagccc gtaatggtcg agggcagata gactttccaa tgctgtattt
    13561 tttccaaacc gagctgggtc atcgcgaaag gcagcgcggt aaacaatgcc atttgcgcgg
    13621 cgtgcagggc gaaaatgccg aaatcgagcg tcagcagcct acggtttttc aaaacttcgc
    13681 ctatgcgcga aggctgcgcc tgcgtatctt cgtgcagctt ggaaacctcc gggtcgggag
    13741 tcatccacgc caccacgccg atactggcaa cggtcaaaat accggtcagc agaaacaagc
    13801 cgcgtacgcc caccgcgtcg gcaatcacag gggcaacgac gaggctgacc gaaaacgtca
    13861 gaccgatact caaaccgatc atcgccatcg cgcgggtacg tacgccgtcg cgcgtcaaat
    13921 ccgccagcag cgcggtaacc gccgcactga ccgctcccgc accctgtatg gcgcgtgcgg
    13981 cgaccagcat gggcagcgta tcggcggcgg cggcaaggaa gctgcccgct gcaaacacaa
    14041 tcagcccgac ataaatggtt ttcttgcgcc cgaacttgtc ggaagcgatg cccaaaggca
    14101 gttgcagcag agcctgtgtc agcccgtaaa tgcccattgc cagcccgacc agcgttttgt
    14161 tgccttccgc gccgggcagc gaggcggcat acaccgccaa tacgggcagc acgaggaaca
    14221 tacccagcat acgcagcgcg tacacgccgg aaagcgtcgt actggcgcgc cattcgtgcg
    14281 gaaacatttg gatgcggtta tctcttgcca tcatattttt tcagacggca tcaacagttg
    14341 caatgccgtc tgaacttcca gtgaacagat tttcggatta tacaggattc gccgtatttc
    14401 ggttgcggcg cgggttcaaa atcaacgcca ctgccagcgg ttgcgccacg cgcccaaaac
    14461 ggcgttcgga tatttattgc tgcccaagct gccgttaaag cgggcaagcg cgcggacgat
    14521 gttgcctttt tcaagattcc ggtaatggcg caggatggta cagccgtaac gcaggttggt
    14581 gcggatgtcg aacaggttgt gcgccggttt gccgatgtag tttttccaaa acggcataac
    14641 ctgcatcagg ccgcgcgcgc cgacaccgct gattgcatac tggcggaacg cgctttccac
    14701 ctcaatcagc cccaacacaa tctgcgtatc caaaccggcc cggctgcttt cgtactggat
    14761 attgaccagc agcctgcgcc gctcctcctc ctcggggacg aaccttgcca aacgtgccga
    14821 catggcagac aaccaacgtt cgccctcttt cggattgtca aacaccagcc tcggcggatt
    14881 gacgctgccg acagaactcc tcatcacgga agccacatcg tcggcaagcg tttcctcacg
    14941 ttgcgcgccg gcgtgcgcca gaggactgag caacaacgca ccggcggcac acaacaggcg
    15001 gcggcgttgc agattaacgg gtagggtatc ggtcggtttt ttcataggga acgggggcgc
    15061 gtccggacgt ttcagacggc attaaatatt caaacagaca atgattgctt ccaacgcgaa
    15121 aaaccgcgca caaaatccaa gcgcggcata tcgccctgcc cttttcgggc aaaccttgat
    15181 tttaccgccc tcaagaacgc ttgtccaaac aggcacaggc aacaccaccc gggcattttc
    15241 cgttttcacc ggttatccgt cgtccggatt atgcagcagc accatcagcg catcacgctt
    15301 ttcgggcggc agcaggc
//
LOCUS       DAWFAN010000033        14917 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWFAN010000033 DAWFAN010000000
VERSION     DAWFAN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14917
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.033"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGO65_000737"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGO65_000737"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0327057.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1965..3572)
                     /gene="aceF"
                     /locus_tag="WGO65_000738"
     CDS             complement(1965..3572)
                     /gene="aceF"
                     /locus_tag="WGO65_000738"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ0327058.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGTAIIEVETAGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQ
                     GGAAKPAAAGASLGSSLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPIWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3722..6385)
                     /gene="aceE"
                     /locus_tag="WGO65_000739"
     CDS             complement(3722..6385)
                     /gene="aceE"
                     /locus_tag="WGO65_000739"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ0327059.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7079..8791
                     /locus_tag="WGO65_000740"
     CDS             7079..8791
                     /locus_tag="WGO65_000740"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ0327060.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFKGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            8854..9510
                     /locus_tag="WGO65_000741"
     CDS             8854..9510
                     /locus_tag="WGO65_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ0327061.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGMGEGEDILGCVAGYGVGLDLTA
                     RDIQCHLKAQGLPWLKAKGFRHSACVSDFAAADRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9520..9975)
                     /gene="ruvX"
                     /locus_tag="WGO65_000742"
     CDS             complement(9520..9975)
                     /gene="ruvX"
                     /locus_tag="WGO65_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ0327062.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9968..10516)
                     /locus_tag="WGO65_000743"
     CDS             complement(9968..10516)
                     /locus_tag="WGO65_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ0327063.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10518..11078)
                     /locus_tag="WGO65_000744"
     CDS             complement(10518..11078)
                     /locus_tag="WGO65_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ0327064.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11406..13211
                     /locus_tag="WGO65_000745"
     CDS             11406..13211
                     /locus_tag="WGO65_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0327065.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13338..14822
                     /locus_tag="WGO65_000746"
     CDS             13338..14822
                     /locus_tag="WGO65_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ0327066.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKLIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQEKTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNGETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3536 a         3696 c         4208 g         3477 t
ORIGIN      
        1 tcggagaaga tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccta
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatca aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggacaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcagca gcaacgtggc
      541 cttcgtgaac ggctttgtgt gccaacattg gttgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggtaacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtaacgttgc ggctctttag gcgcatttgc accttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttca ccaagtcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagtgg
     1141 ctgcctgttc gtacgcgtcg ccggcagtca gcgacacttc caagtggtgc gggtctaaga
     1201 attgcccgtc gccttggata acgtccactt tacggctttt cgccatacct gccaaaccgc
     1261 ccgtgaggcg ggaaactacg ccgtctttgt aggcgcgaag catatcgatg tcgagttccg
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacaa ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg accgccaccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 ccgcttcggc tttaggagcg gcagccgtgc cttcagcttc aacgacaaca atcaaaccgc
     1681 cttcggagat tttgtcgccg actttgactt tcacttcttt gaccacgccc gcaacttcgg
     1741 caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat cagggtgtcg tcaacggcga
     1801 tggtgtcgcc cacgtttact tcaaccgcga taatatctac attttcgtgt ccgccaatgt
     1861 cgggcacttt caattcaact aagctcatgt ctcatctttc tgaatgatgt tcaaacttcc
     1921 ttttcagacg gcacagaccg ctatgccgtc tgaagccgat cgggttacag ggtaatgcgg
     1981 cggaagtctt tcaacagctt cgccaagaat acggtaaagc gcataccggc cgcaccgtcg
     2041 atgacgcggt ggtcgaagga caggctcaac gggcacatca ggcgcggggc aaactctttg
     2101 ccgttccaaa tcggtttgat ttgggatttg cacacgccca agatggcgac ttcgggagcg
     2161 tttacaatcg gcgtgaagcc tgtgccgccg ataccgccca agctggaaat ggtaaagcac
     2221 gcgccttgca tttcttgcgg tttgagcttg ccttcgcggg cttttttgga caattcggtc
     2281 agctcttggc tgatttgttt caagcctttt tgatccacgt ctttgatgac gggaacaacc
     2341 aagccgttcg gcgtatcggc tgcgaaaccg atgttgaagt agtttttcag caccaggttg
     2401 tcgccgtcca gagaagcgtt gaattcgggg aaggctttca gcgcggaaac ggaggctttg
     2461 atgatgaacg ccaacggtga cagtttcacg ccttcgcgtt cccattcttt gttcagttgt
     2521 ttgcggaatt cttccagctc ggtcatatct gcttcttcgt gtacggtaac gtggggaatc
     2581 accacccagt tgcgagacag gttttgaccg gagattttct taatgcggga caattcttta
     2641 acttcgacat tgccgaattt ggagaagtcc actttaggcc acggcagcaa gtccagactg
     2701 ctgcccaaag atgcgccggc tgcggcaggt tttgccgcgc caccctgcat tacggatttc
     2761 acaaaggctt tgatgtcgtc gcccacgata cggcctttca agccggtacc tttgacttgg
     2821 cccaaatcca cgcccaattc gcgcgccagt ttgcgtgcgg aagggccggc gtgtgctttg
     2881 gcgaaagcgg cctcgtcgat tttggcagcg gcaggtgcag aaggtgcagg cgcggcggcg
     2941 ggtgcggcgg cagcagtcgg aggcggagcg gcagccggtg cggcagcttg agcggcttga
     3001 gcaggggctg ctgcggcaga gccggcggtt tctacttcga taatggcagt accttcggac
     3061 actttgtcgc cgacttttaa gaatacggct ttaacgacac cggcagcggt acaaggtacg
     3121 tccatcgttg ctttgtcggt ttccaaagta atcagcgtgt cgtcttcggc aacggtatcg
     3181 ccgactttga tctcgacggc gattacatcc acatcggtat ggccgccgat gtcgggaacg
     3241 gctacttgaa cggttgcacc gcctgcggca acgggtgcgg cagcaggtgc aggttgtgct
     3301 tcggcagcag caggagcggc ttcggcagcg gcggcaccgg tttcaacggt caaaattacg
     3361 ccgccttcgg agattttgtc gccgactttg actttaactt ctttcacgac accggccgca
     3421 tcggcaggca catccatcgt ggctttgtcg gtttccaaag taatcagggt gtcgtcaacg
     3481 gcgatggtgt cgcccgcttt aacttctacg gcgatgatgt cgacgttttc gtgaccgccg
     3541 atatcgggga ctttgatttc tacgatactc atttgagttc ctttaatctt tttcagttgg
     3601 atgccgtctg aaaactgctt cacgcggcat cggtacggtt tgtctgttca aaccattcgg
     3661 gcattcggtt ttcagacggc atgatgtctg aatggggcaa acaggccgtc tgaaacatca
     3721 atcagcgttt ccagctaggg gctgaatcgg ctttgatgcc gtatttttca atggcttgtt
     3781 gaacagtttc tttgctgact ttgccttgtt ccgccaatgc ggccaatgct gctacggcaa
     3841 cgttgtagcg atccacttca aagaagcggc gcaggttggc gcgactgtcg gaacggccga
     3901 aaccgtcagt gcccaagacg tggtagtcgt tagggatgta ggcacggatg cggtcagcat
     3961 agctgcggat atagtcggta gcggcgataa ccggaccgtc atgaccttgc agttgagagg
     4021 taacgaaagg cactttttca gcttccagcg gatgcaggcg gttgaagcgt tcggtttcga
     4081 tggcgtcgcg gtgcagcagg ttgaaggacg ggcaagacca gatgtctgct tccacgccga
     4141 agtcggcttt cagcagctcg gcaccggcaa tcacttcttg cagaatcgta ccggaaccca
     4201 tcagttggac tttcttgtcg cctttgccgc cggctttcag caggtacata cctttcaaga
     4261 tgtcttgttc cgcaccttcg ggcatatccg gatgggtgta gttctcgttc atcagggtga
     4321 tgtagtagaa cacgtcttca ttattggcat acatacggcg cagaccgtct tgtacgatga
     4381 cggcgacttc gtattggaaa gtcgggtcat aagatacgca gttcggaatc aggtcggcct
     4441 ggatgtggct gtggccgtct tcgtgttgca ggccttcgcc gttcagcgtc gtacggccgg
     4501 cagtaccgcc cagcaggaag ccgcgcgcgt gcatatcgcc cgccgcccaa gccaagtcgc
     4561 cgatacgttg gaaaccgaac atagaatagt aaatgtagaa cggaatcatg gcgaagttgc
     4621 tgttggcgta gctggttgca gccgcaatcc agtcggccat cgcgcccggt tcgttaatac
     4681 cttcttgcaa gatttgaccg tcaacggatt ctttatagaa catcagttgg tctttgtctt
     4741 gaggggtata ttgctgacct ttcggattcc aaataccgta ttggcggaac ataccttcca
     4801 tgccgaaagt acggctttcg tcgggaacga taggtacgac gcgtttgccg atttttttgt
     4861 ctttcagcaa agtggacagg atgcggacga atgccatcgt ggtcgagaac tcgcgttcac
     4921 cgctggattt gagttgtgcg tcgaatgctg acagctcggg cacttccaat acttcctgcg
     4981 tcggtttgcg ttgcggcagg tagccgccca aagcatcgcg gcgtgcgtgc aggtatttgt
     5041 attcttccgt atcgggggca aaagtcaggt aaggcagatc gccgctttcg atttgttcgt
     5101 cggtaaccgg aatgtcaaag cggtcgcgga attgtttcag ggacgctttg tccatttttt
     5161 tggcttggtg ggcaacgttc tgaccttcgc cggatgcgcc cataccgtaa cctttaatgg
     5221 ttttcgccaa gatgacggta ggtttgccgt cggcatggtt cgctgcgcgg tcgtaggcgt
     5281 tgtacacttt ttgcgggtcg tggccgccgc ggttcaatgc ccagagttgc tcatcggtca
     5341 tatcggcaac caatgctttc agttcgggcg tattgaagaa gtgttcgcgc acatacgcgc
     5401 cgtctttgga tttgtaagtt tggtagtcgc cgtccaaaca ttcttccata cgttggcgca
     5461 ggataccgtc tttgtctttc gccaagaggc ggtcccagcg gcggccccaa atgactttga
     5521 cgacattcca gccggcgccg gcaaagttgc cttccaattc ttggatgatt ttgccgttgc
     5581 cgcgtaccgg accgtccaag cgttgcagat tgcagttgat gacgaaaatc aggttgtcca
     5641 agccttcgcg tgcagccagt gcgattgcac cttgagattc gggttcgtcc atttcgccgt
     5701 cgccgcagaa acaccatact ttacggcctt tggtttttgc caaaccacgc gattccaagt
     5761 atttcaggaa acgcgcctga taaatcgcca tgatgggccc caagcccatg gatacggtcg
     5821 ggaactgcca aaagtcgggc aagaggtggg ggtgcggata ggaaggcaga ccgtgtccgt
     5881 ccacttcttg gcggaagttg ttcagctggt cttcggtcag acggccctcg acgaatgcgc
     5941 gtgcatagat gcccggggcg acgtgacctt ggaagaagac caaatcgcct tcttcgcctt
     6001 cgcctttggc tttccaaaag tggttgaaac cgacttcgta catggtggcg gcagattgga
     6061 aagatgcgat gtgcccaccc agttccaaat ctttcttgcc ggcgcgcaat acgatggcgg
     6121 cggcgttcca gcgcacgaat gcgcggatgc ggtgttcgat gttttggtcg cccggaatgc
     6181 ctttttcgtt ttcaaccgaa acggtattca aatacggggt ggtcgtgccg tgtggcatac
     6241 gtacgccctt gtcgcggcag tatttgacca ggttttccaa gaggtattgc gcgcgttcgc
     6301 cgccttcata ttcgaggacg gagcttaaag cgtccagcca ctcttgggtt tcgatagggt
     6361 caacatcgtg taattgggtg gacatagtat ctatccttta tgttgagtgt ttaatcggca
     6421 gggagggtgt agccaacccc tttattttcg tttgtgaaaa ataataagca aaaacgaaaa
     6481 tttctaaatc aaaccgaaaa aaataattta agtttaatgt tttcaaaaaa ttataaaagt
     6541 tttggaaaca agggaaaact tgaaaaaaat gttcgatctg ctccacacga aatcctatca
     6601 gcaatcgggt ctgctggcaa ataaccatgc ctgtaaaagc aggttttata ctgaagttgg
     6661 cgtttggtaa ggtcaagtct ttttgatttc gaaatattct gtttgatttt ggcgatattt
     6721 tttcggaatt gtggtatatc gaatcattat gtctctgtcg gcaattttcc tattgtagaa
     6781 aatctataca caagctttgt ggataaccgt gttgataagc cacggcttgt gttaaaaaag
     6841 tcttacgcaa caaatgtacg cttattttga ttaaaaaata ggcacattta aatctataaa
     6901 aatcatgtgc ttatgttcaa tatgatgttt ctggtcatgt ttttgccgtc tgttatggga
     6961 tttgtcccgc gcttggggca gggggtgaat tttggaggtt ggtgcaaaac ctgctttttt
     7021 cgcgttacaa tctgcacccg atttactttt gaatttgcct gtttggaaaa atacttttat
     7081 gaaagccagc caattcttta tctctacttt aaaagaagcc cctgccgaag ccgcgcttgc
     7141 cagccacaag ctgatgattc gcgccggcct gattaaagcc aacgcgtccg gcctttatac
     7201 ttggatgccg atggggctgc gcgtgttacg caaagtcgaa aacgtcgtgc gcgaggaaat
     7261 ggcgcgcgcg ggcagcgtgg agctgctgat gccggtggtg cagcctgccg agctgtggca
     7321 ggaatccggc cgctgggaat tttacggtaa agaactgctg cgcctgaaag accgccacga
     7381 ccgcgatttc tgcatgggcc cgacctgcga ggaagtcatc gccgacatcg tgcgcaaaga
     7441 aatcaacagc tacaaacagc tgccgaaaaa cttttaccac atccaaacca aattccgcga
     7501 cgaagtgcgc ccgcgtttcg gcgtgatgcg cgcgcgcgag ttcgtcatga aagacgctta
     7561 ttccttccat gccgactacg cctcgcttca gacgacctat gatgccatgt atgacgctta
     7621 ctgccgtatc ttcacccgtc tgggcttggc attccgcccc gtcgccgcag acaccggcag
     7681 catcggcggt acgggttcgc acgagtttca agtgttggcg gaaagcggcg aagatgttat
     7741 tgcgtacagc gacacttccg attacgccgc caatatcgag ttggcaccga ccttgccgct
     7801 taaaggcgaa cgcgccgctg ctcaggctgt gttgaccaaa gtacatacac caaacgtcaa
     7861 aaccattgag tctttggttg aattcctgaa tattccggtt gaacaaaccc tcaaatccat
     7921 cgtggtcgaa ggcgaaaacg aaggcgaaat cgtcttatta ctgttgcgcg gcgaccatga
     7981 gttcaacgac atcaaggcag aaaaactggc gggcgtgaaa tcgccattga cgatggcaag
     8041 ccctgccgcg attgtcgaac agttcggcgc aaacggcggc tctcttggtc ctgtcggctt
     8101 caaaggcaaa gtctatgccg atttcgctac cgaaaaaggc gcggactggg tcatcggtgc
     8161 gaacgaagac ggctaccact ataccggctt caacttcggt cgcgatgccg ccgagcctga
     8221 gtttgtcgat ttgcgcaacg tcgtcgaagg cgacgaaagc cccgacggac aaggccgtct
     8281 gaaactggcg cgcggcattg aagtcggaca cgtcttccag ttgcgcgaca aatatactaa
     8341 agccatgaac gtcagcttcc tcgacaacaa cggcaaatcg caaattatgg aaatgggctg
     8401 ctacggcatc ggcatcaccc gcgtcgttgc cgccgccatc gagcagaata acgacgaaaa
     8461 aggcatcatc tggaccaaag caatggcgcc gtttgaagtc gttatcgtgc cgatgaacta
     8521 caaaaaatca gacaccgtac gcgaagccgc cgacaaaatc tatgccgaat tgctggcggc
     8581 aggcgcggat gtgctgctgg atgaccgcga cgaacgcgca ggcgtattgc tgaacgattc
     8641 cgagctgttg ggtattccgc accgcatcgt catcggcgac cgcgctttga aagaaggcaa
     8701 tgtcgaatac gccgaacgcc gcaacaacga agcgcaggca gttgcaatcg gagaaattgt
     8761 tgcgcgtgta acagcttcat taaatgggta agcttggtca aatgccgtct gaaaataatt
     8821 tcagacggca tttttatttg gggagaaagt gaagtggctt cagtattttt agaaggagaa
     8881 gcagtcgagg tcggcaatat tttctgtatc ggcaggaact atgccgcaca tattgaagag
     8941 ttaaaaaacg aaataccttc cgaacctgtg gtgtttatga agccgtcagg cagcattttg
     9001 aatagcggcg gtacaatctt gctgcccgag tttagtaggg atgtgcagtt tgagtgcgaa
     9061 ctcgtgctgc tggtcggtaa ggattcagac ggcatgggag agggtgaaga tattttggga
     9121 tgtgtggcgg ggtacggcgt gggtttggat ttgaccgcgc gagacatcca atgccatctg
     9181 aaggcgcagg ggctgccctg gctgaaggca aaagggttca ggcattccgc gtgcgtgtcg
     9241 gattttgcgg cggcagacag aatcggcaat cccgagaagg tgttgttttc attaaaacaa
     9301 aacggcgtat tgaaacagcg cggggatacc ggtttgatga tttacccgat tcgggaaatt
     9361 ttacacaaac tggcggcgga ttacggtttg gggaaagggg atttggtgtt taccggaacg
     9421 ccctccggcg tgggcgcaat cggtgcgggg gacaatttgg cattggagct ggacggcttg
     9481 gtgcgcgcct ctttcacggt agggtgttaa ccgcgccgct taaccctcac gcccgttgaa
     9541 acattccgcc ggaccgccct cgaaaaaacc gtgcaagatg gcttgcgccg ccacttggtc
     9601 gagcaccgat ttgcgttttt tgccgaagac ctgtgcttcc gaaagcaggc tttcggcata
     9661 gacggacgac agccgttcgt caacccaata gacggggaga ttgaacctgc cgttcagcct
     9721 gcgtccgaac ttgcgcgaca ggtgcgtcat ttcatgtttc gtgccgtcgg tatgcacggg
     9781 cagtccgacg acaaaataac gcggctgcca ttcttgaacc agcttggcga ttgccgcgaa
     9841 cttttcatcg ttgctgccgc cggtaacggt gctcaaagga tgggatagcc ctaattccgc
     9901 gtctccttgt gccacgccga tacgcgcttc gccgaagtcg aatgccagtg ccgttccttt
     9961 tggaatttta tgcatggccg gctcccgaaa acagggcgag cgggtcgatg ccgagtttgg
    10021 cgaatgcggc ggcgtaacgg tgttcgtagg ggatgtcgaa caggatgtgt tcgtcggcgg
    10081 gaacagtcag ccacgcattg tcggcaagtt cgcgttcgag ctggcctttt ccccagcttg
    10141 aatagcctat gctgatcaag gctttgtcaa ccgcaccttc gcgtgaaata ttttcaatca
    10201 cgtctcggga agaagttagc gcgatattgt ctgaaacgcc gatactgctt tgccagttgc
    10261 cgatcggggt atgcacgaca taacctcgct cgacttgcac cggtccgccc atcatcacgc
    10321 tgtcgtgctg catccgcatg gggatgtttt tgccggtggc ggaaaaaatc atgtccatcg
    10381 taatcggaga gggtttgttg atggcgatgc cgagtgcgcc gtcttcatcg tgtttgcaga
    10441 tatagacgac cgattgtgaa aaaaacgcgt cttccatatc gggcatggcg acgagaaagt
    10501 ggttggagag gttcatatca gcgtttgtcg ggattttcta tcgggttgga aatcatgcag
    10561 gcgccggcaa gcaggttgcc ggaagtatcg gcttgcaccc catcggtttg cggtatgccg
    10621 ccgccgaaac aggaaaccgc gcttaaggaa tttttcggcg agccttggat gattttatcg
    10681 ctgtacacca aataggcgaa ggttttgcgt ttggggtcgt aataacggac aatctgccgg
    10741 ctcttgaacg cgaagctcgc accgtgtttg aaaacttctt tcggtttgcg cacggcggtt
    10801 tcgtcaaaag aaatcgaaga tgccgtctga acgcacgaaa ccgatgcgtc ggacgcgtcc
    10861 tcttccaaat tgaccatttc cttcaagccg cctttttttg catacgaaat ataacaggca
    10921 accccttgaa cgtcgggatc gtcgaatcct tccacttcga tacggtcgtt tttgcccagt
    10981 atgttgaaaa cggtacttgc ccgtccgatt ttatcggttt cgccgctgcc gcaggcagtc
    11041 agcaggacgg cggcagacag cagtagcagt ctgttcatca aaatttcctt atcggttgaa
    11101 tccccgtttt ccgtgaaggc ggcagggctt gctttgtttg gaggggcgca cgttcccaaa
    11161 atcgggcaac atacggtatg cggcttaatg tgctaccctg tgtgtcggaa cacaatatcc
    11221 gttgcggcat tgaaaatatg acgcacaata taaggctgtt tgagccgatt gcaagtatcc
    11281 gcgcaaagaa ggcgcaagat tgggcaaacg gcattttcag acggcataac tgacagtata
    11341 atccaaacgt ccggctgctt tcgtgcagcc ttcagtatta tccaactttt tccgaaggtc
    11401 gaaccatgcg ctacaaaccc cttctgcttg ccctgatgct cgttttttcc acgcccgccg
    11461 ttgccgccca cgacgcggca cacaaccgtt ccgccgaagt gaaaaaacag acgaagaaca
    11521 aaaaagaaca gcccgaagcg gcggaaggca aaaaagaaaa aggcaaaaat ggcgcagtga
    11581 aagataaaaa aacaggcggc aaagaggcgg caaaagaggg caaagagtcc aaaaaaaccg
    11641 ccaaaaaccg caaagaagca gagaaggagg cgacatccag gcagtctgcg cgcaaaggac
    11701 gcgaagggga taagaaatcg aaggcggaac acaaaaaggc acatggcaag cccgtgtccg
    11761 gatccaaaga aaaaaacgca aaaacacagc ctgaaaacaa acaaggcaaa aaagaggcaa
    11821 aaggacaggg caatccgcgc aagggcggca aggcggaaaa agacactgtt tctgcaaata
    11881 aaaaagtccg ttccgacaag aacggcaaag cagtgaaaca ggacaaaaaa tacagggaag
    11941 agaaaaatgc caaaaccgat tccgacgaat tgaaagccgc cgttgccgct gccaccaatg
    12001 atgtcgaaaa caaaaaagcc ctgctcaaac aaagcgaagg aatgctgctt catgtcagca
    12061 attccctcaa acagcttcag gaagagcgta tccgccaaga acgtatccgc caagagcgta
    12121 tccgtcaggc gcgcggcaac cttgcttccg tcaaccgcaa acagcgcgag gcttgggaca
    12181 agttccaaaa actcaatacc gagctgaacc gtttgaaaac ggaagtcgcc gctacgaaag
    12241 cgcagatttc ccgtttcgta tcggggaact ataaaaacag ccagccgaat gcggttgccc
    12301 tgttcctgaa aaacgccgaa ccgggtcaga aaaaccgctt tttgcgttat acgcgttatg
    12361 taaacgcctc caatcgggaa gttgtcaagg atttggaaaa acagcagaag gctttggcgg
    12421 tacaagagca gaaaatcaac aatgagcttg cccgtttgaa gaaaattcag gcaaacgtgc
    12481 aatccctgct gaaaaaacag ggtgtaaccg atgcggcgga acagacggaa agccgcagac
    12541 agaatgccaa aatcgccaaa gatgcccgaa aactgctgga acagaaaggg aacgagcagc
    12601 agctgaacaa gctcttgagc aatttggaga agaaaaaggc cgaacaccgc attcaggatg
    12661 cggaagcaaa aagaaaattg gctgaagcca gactggcggc agccgaaaaa gccagaaaag
    12721 aagcggcgca gcagaaggct gaagcacgac gtgcggaaat gtccaacctg accgccgaag
    12781 acaggaacat ccaagcgcct tcggttatgg gtatcggcag tgccgacggt ttcagccgca
    12841 tgcaaggacg tttgaaaaaa ccggttgacg gtgtgccgac cggacttttc gggcagaacc
    12901 ggagcggcgg cgatgtttgg aaaggcgtgt tctattccac tgcaccggca acggttgaaa
    12961 gcattgcgcc gggaacggta agctatgcgg acgagttgga cggctacggc aaagtggtcg
    13021 tggtcgatca cggcgagaac tacatcagca tctatgccgg tttgagcgaa atttccgtcg
    13081 gcaagggtta tatggtcgcg gcaggaagca aaatcggctc gagcgggtcg ctgccggacg
    13141 gggaagaggg gctttacctg caaatacgtt atcaaggtca ggtattgaac ccttcgagct
    13201 ggatacgttg atcggattgc cgaaacggta ggctttatgc aaatcgtgcc gatatggtgc
    13261 gctgttccgt gaagtttgcc gtttgccgta cctgtatttt ccggtttttg gaaatcaaga
    13321 gagaaagtta aagaagaatg tcgaaacctg tttttaagaa aatcgcactt tatactttgg
    13381 gtgcaatcag cggcgtggcc gtcagtctgg cggtgcaggg ttttgccgcc gagaaggaca
    13441 ggcgggataa cgaagtcctg ccggtgcaat ccatccgcac aatggcggag gtttacggtc
    13501 aaatcaaggc aaactactat caggacaaac ccgatgccga tttgtttgaa ggtgcgatga
    13561 agggtatggt ggccggtttg gatccgcatt ccgaatatat ggataaaaaa ggttatgccg
    13621 agataaagga gtccaccagc ggcgaatttg gcggcttggg gatggaaatc gggcaggaag
    13681 acggatttgt caaagtggtt tcgccgattg aggacacgcc tgcggaacgg gcgggggtga
    13741 aaagcggcga tttcattgtg aaaatcgata atgtttcgac acgcggcatg acggtcagcg
    13801 aagcggtgaa gaaaatgcgg ggcaagccgg gtacgaagat tactttgacg ctgtcgcgca
    13861 aaaatgccga caagctgata gtcgtcaacc tgacccgtgc cattattaaa gtgaaaagcg
    13921 tccgccatca cctgatcgaa cccgattacg gctatatccg cgtgtcgcag ttccaagaga
    13981 agacggtcga aagcgtcaat accgccgcaa aagagctggt aaaggaaaat aaaggaaaac
    14041 cgctcaaggg gctggtgttg gatttgcgcg acgaccccgg cgggcttttg actggcgcgg
    14101 tcggcgtgtc ggcggcattt ctgccgtctg aagcagtcgt cgtcagcacc aagggacgcg
    14161 acggcaaaga ccgcatggta ctgaaagcca ttcctgaaga ttatgtgtac gggatgggcg
    14221 gcgattcgtt ggcgggcatt cctgccgagt tgaaaaccat accgatgacg gtattggtca
    14281 attccggttc ggcttccgcg tcggagattg tcgcaggtgc attgcaggat cataaacgcg
    14341 cggtcatcgt cggtacgcag agcttcggca aaggttcggt tcagactttg attcctttgt
    14401 ccaacggcag cgcggtcaag ctgacaacgg cactgtatta tacgccgaac gaccgttcta
    14461 ttcaggcgca ggggattgtt cccgatgtcg aagtaaaaga taaggaacgc atttttgaaa
    14521 gccgcgaggc ggatttggtc ggacacatcg gcaatccctt gggcggcgag gatgtgaacg
    14581 gtgaaaccct tgccgtgccg cttgaaaaag atgcggataa gcccgctgta aaagaaaaag
    14641 gtaaaaagaa aaaggacgag gatttgtctt caaggcggat tcccaaccct gccaaagacg
    14701 accagttgcg gaaagctttg gatttagtca agtcgcccga gcagtggcag aagtctttgg
    14761 ggctggcggc gaaaaagccg gtttcaaata aagataagaa agataaaaaa gataagaagt
    14821 aggttttgac gatgccgtct gaaaggattt cagacggcat attatgtttg aaaggacagg
    14881 ttatgagcga ttttttaccg gaatttgaac cgtgcgg
//
LOCUS       DAWFAN010000034        14911 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWFAN010000034 DAWFAN010000000
VERSION     DAWFAN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.034"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(92..880)
                     /locus_tag="WGO65_000747"
     CDS             complement(92..880)
                     /locus_tag="WGO65_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0327067.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(882..1757)
                     /gene="bioH"
                     /locus_tag="WGO65_000748"
     CDS             complement(882..1757)
                     /gene="bioH"
                     /locus_tag="WGO65_000748"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ0327068.1"
                     /translation="MRYAAKSSRQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKS
                     MPDAVKKVYLIHGWGANRHVFDDLMPRLPATWAVSAVDLPGHGDAPFVQPFDIEAAAD
                     AVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTAAEDYPEGLAAP
                     ALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLARCGTPSALQEALDAA
                     ERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLVVMEKAAHAPFLSH
                     AEAFAALYRDFVEGV"
     gene            1852..2349
                     /locus_tag="WGO65_000749"
     CDS             1852..2349
                     /locus_tag="WGO65_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0327069.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWRTLPLHTVFRRHRPPQSTLKG
                     GERRRNIKNAFEIRTPPPKNCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            2414..2878
                     /gene="trmL"
                     /locus_tag="WGO65_000750"
     CDS             2414..2878
                     /gene="trmL"
                     /locus_tag="WGO65_000750"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ0327070.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            2889..3011
                     /locus_tag="WGO65_000751"
     CDS             2889..3011
                     /locus_tag="WGO65_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0327071.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3008..3727
                     /locus_tag="WGO65_000752"
     CDS             3008..3727
                     /locus_tag="WGO65_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ0327072.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANCSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMIQTVLTAG"
     gene            3781..4107
                     /locus_tag="WGO65_000753"
     CDS             3781..4107
                     /locus_tag="WGO65_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0327073.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4189..4773
                     /gene="ruvA"
                     /locus_tag="WGO65_000754"
     CDS             4189..4773
                     /gene="ruvA"
                     /locus_tag="WGO65_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ0327074.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVSGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHAVTDGLFAAAPAADETEDIVGTLLA
                     LGYSEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            4773..4910
                     /locus_tag="WGO65_000755"
     CDS             4773..4910
                     /locus_tag="WGO65_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327075.1"
                     /translation="MPSEGGATFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            4936..6792
                     /locus_tag="WGO65_000756"
     CDS             4936..6792
                     /locus_tag="WGO65_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0327076.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLYSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPQALPLNVPQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGITQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            6797..7720
                     /gene="rsgA"
                     /locus_tag="WGO65_000757"
     CDS             6797..7720
                     /gene="rsgA"
                     /locus_tag="WGO65_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ0327077.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSMRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            7982..8206
                     /locus_tag="WGO65_000758"
     CDS             7982..8206
                     /locus_tag="WGO65_000758"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ0327078.1"
                     /translation="MKKNAPKSFEEALSRLESLTQAMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            8190..9086
                     /locus_tag="WGO65_000759"
     CDS             8190..9086
                     /locus_tag="WGO65_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ0327079.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAV
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9195..9494)
                     /locus_tag="WGO65_000760"
     CDS             complement(9195..9494)
                     /locus_tag="WGO65_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ0327080.1"
                     /translation="MTASMYILLLLALIFANAPFLTTRLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(9606..11051)
                     /gene="nuoN"
                     /locus_tag="WGO65_000761"
     CDS             complement(9606..11051)
                     /gene="nuoN"
                     /locus_tag="WGO65_000761"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ0327081.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALMFTLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRAHPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(11061..12557)
                     /locus_tag="WGO65_000762"
     CDS             complement(11061..12557)
                     /locus_tag="WGO65_000762"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ0327082.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGSDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFTILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAQVAQSKM"
     gene            complement(12646..14670)
                     /gene="nuoL"
                     /locus_tag="WGO65_000763"
     CDS             complement(12646..14670)
                     /gene="nuoL"
                     /locus_tag="WGO65_000763"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ0327083.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQTATIQ
                     LFPSVEWSLITVTCLLLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLFVTLPLILLAIPSVIIGYVAI
                     GPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(14671..>14911)
                     /locus_tag="WGO65_000764"
     CDS             complement(14671..>14911)
                     /locus_tag="WGO65_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEZ0327084.1"
                     /translation="NILFIGIVFLFIQIAAYFDLKLILENQKTLYLIPLLPPAANIIV
                     LETAYLKTGRSASMIAYGTIFSLIAISIYILLVQI"
BASE COUNT         3794 a         4439 c         3854 g         2824 t
ORIGIN      
        1 cactatacat cggcatttcc tttagttctg tcggttttca caaacaaatg ccgtctgaaa
       61 cttggaaacg gcttcagacg gcatctgcac atcatctctt cggaaaaaac tgcaccacgt
      121 tctccccttg cggcagcatc agttttttaa cggcgtgtcc gtacactgtt tccagcgtaa
      181 tactcaaacc gccttcccgt tcaaatattg tcccgacaca cgaacgcgcg gcgttttcat
      241 cgttccacga catcgcccgc caaacgccca gcgtgtccat atccgcccaa aacgtttccg
      301 cctttttgta atccaacacc agcttcgccg tatcggtaac ggggtcgtaa aacccgtttt
      361 cagcaagcat atcgcccaag tcgtgcatat cggggaaaag cgcgctgcgg ctttcaatgc
      421 cgttttcttt cagacggcat ttcagttcgg taaaagtgtc gcgcccgaag caggtaaaaa
      481 acagcagccc gtccgtcttc aaggcgcgcg cccagttgtg cagcacagga aggatttgtt
      541 ccgccgccaa cagtccgaga ttcgaccaca acatatcggc acacgcttcg ggcagcggcg
      601 cggtcgggga ttggcagtgt tgcaccacgc ccttacccgt aaacctttgc caaaaaccgc
      661 ccttgcgcac ggctgcggca gccgccaaaa aatccgcacg ggaatcgtat tcttcaaata
      721 ccgcctgcgg atatcgtttc gccagcaggc tgcggctgat gtccgcatcc gccccggcaa
      781 gcaggatatg tttgggcgcg ttgcggacga gtgtcagccg ttggtcggca tgttcggcaa
      841 gatggcggtg aacctgccaa catttgtcct gcggattcat gtcaaacccc ttcgacaaag
      901 tcgcggtaca gcgcggcaaa cgcttccgca tggctcaaaa acggcgcgtg tgccgccttt
      961 tccatcacaa ccagcctgct gcctttcaaa tgccgatgca gatactcacc catacgcggc
     1021 ggtgtaatcg cgtctttgcc gccgaacacc agcagtaccg gaacatctat cttgtccaac
     1081 aaatgccgcg catccgccct ttccgccgcg tccaacgcct cctgcagggc ggagggcgtg
     1141 ccgcagcgcg ccaaatcggg cagaatcctg ccgatgattt cggcggcatc aggcgtgtgc
     1201 agaagctgca attgtagaaa ctgtttgata tgtttggcat aatccgttcg gaacgcgccg
     1261 accattttgc ccagtgcagg cgcggcaagc ccttcgggat aatcttcggc agccgtcagc
     1321 cgtgcgaaac tcgccgtcag gcagagcgaa cggactttgt cgggatggcg cgccgccaga
     1381 tacagcgcga ccaatccgcc gagcgaccag ccgagaatgt cggcgggcgt atcgatttgc
     1441 gcggcaacag cgtcggcggc tgcctcaata tcgaagggtt gcacgaaggg cgcgtcgccg
     1501 tgtccgggca aatcgacggc ggacaccgcc cacgttgcag gcaggcgcgg catcaaatcg
     1561 tcaaacacgt ggcggttcgc cccccaaccg tgtatcaggt aaactttttt gacggcatca
     1621 ggcatggatt ttctctcttg ttggcggcgc atcgcagacg cgcccactat caggcgttgc
     1681 gtattatgcc acggttcgtc cggcgtttca gacggcatct gcgccggctg ccgcgacgat
     1741 ttggcggcat accgcaccga cgcggcaaac agctgccccc tgtgtttcat gcacgttcaa
     1801 ggcggcgcgg tgtgcggcgg ctgtcagaaa aaaccgcccg cattcgaccg gatgtgggct
     1861 tcgctgcatt acgaaccgcc cgtcagcaat atgatacacg cgctgaagca cttggctgat
     1921 ttgagcatgg tgcagccgct ggcagacctg atgatgcaga atccgcccga ccggcttgca
     1981 gacgaatgtt tcgatttcgt cctgcccgta ccgctaagca gggagcggct gctgcaacgc
     2041 gggttcaacc aaagtgaaag catcgtcggg ctgctggctc aacgctacgg ttggcgcacg
     2101 ctgcccctgc ataccgtatt ccgccgccac cggccgccgc aaagcacgct caaaggcggc
     2161 gaacggcggc gaaatatcaa aaacgccttt gaaatccgca caccgccgcc gaaaaactgt
     2221 aatattttgt taatcgacga tgtctttacc accggcgcga cgctggacga attggcaaag
     2281 acgctgaaaa aatcgggcgc aaaccgaatc tgctgctgga cgctggcacg cacgccaatg
     2341 aaaaaataac taaatttttt gacaccccat ccgccttgtg gcaaggttac gcgcctataa
     2401 gtgattgata tttatgttta ccatcgtttt ataccaaccg gaaatccccc cgaacacggg
     2461 caacatcatc cgcctgtgcg ccaataccgg cgcggatttg caccttgtca aaccgctcgg
     2521 cttcccattg gattccgcca aaatgaaacg cgccgggctc gactaccacg aattcgccag
     2581 cctgacggtg tacgaaaact tcgacgactg cctcaaggca ctcgcaggca ggcgcatttt
     2641 cgccctgacc accaaaggca cggcgcgccc cgatgaaacc gcgtttcaaa aaggcgacgt
     2701 tttactgttc gggccggaaa cgcgcggact gcctgccgac atcctcgaca gcctgcccgc
     2761 cgcgcaaaaa atccgcctgc cgatgcagcc cggcagccgg agtatgaacc tttccaatac
     2821 cgttgccgtg attctctttg aagcgtggcg gcaacacggt tacgcaggcg gcgtttgaac
     2881 gcaagttcat gccgtctgaa aacctatccg gacacattcc gaaccgccgt ccgcactgta
     2941 cggcggttcg gaacgtatcc ggcgggcatg atgcccattc gtcttcaact caagaacgga
     3001 acacgttttg actttaaccc gaaaaaccct tttccttctc accgccgcat tcggcacaca
     3061 ctcccttcag acggcatccg ccgacgcagt ggtcaaggca gaaaaactgc acgcctccgc
     3121 caactgcagc tacaaagtcg ccggaaaacg ctacacgccg aaaaaccaag tcgccgaatt
     3181 cacgcaaacc ggcaacgcct cgtggtacgg cggcaggttt cacgggcgca aaacttccgg
     3241 cggagaacga tacgatatga acgcctttac cgccgcccac aaaaccctgc ccatccccag
     3301 ctatgtgcgc gtaaccaata ccaaaaacgg caaaagcgtc atcgtccgcg tcaacgaccg
     3361 cggccccttc cacggcaacc gcatcatcga cgtatccaaa gccgccgcgc aaaaattggg
     3421 ctttgtcagc caagggacgg cgcacgtcaa aatcgaacaa atcgtcccgg gcaaatccgc
     3481 accggttgcc gaaaacaaag acatctttat cgacttgaaa tctttcggta cggaacacga
     3541 agcacaagcc tatctgaacc aagccgccca aaacttcgcc gcttcgacat caagcccgaa
     3601 cctcgccatt gaaaaacgcc gttacgaata cgtcgtcaaa atgggtccgt ttgcctcgca
     3661 ggaacgcgcc gccgaggccg aagctcaggc gcgcggtatg attcagacgg tattgaccgc
     3721 cggttgatgt cccgccttta cgtctttcaa acaccaccag atacttcgca ggagcaaaac
     3781 atgaacttca aacgccttct cttgaccgcc gccgcaaccg cactgatggg catttccgcc
     3841 cccgcactcg cccaccacga cggacacggc gatgacgacc acggacacgc cgcacaccaa
     3901 cacagcaaac aagacaaaat catcagccgc gcccaagccg aaaaagcagc gttggcgcgt
     3961 gtcggcggca aaatcaccga catcgatctc gaacacgaca acggccgtcc gcactatgat
     4021 gtcgaaatcg tcaaaaacgg acaggaatac aaagtcgttg tcgatgcccg taccggccgc
     4081 gtgatttcct cccgccgcga cgactgaatt tgatacaatc cgtgccgtct gaagcccgaa
     4141 ccggtttcag acggcatttt gcacccgaca cttcaggatt cggcatacat gatcagcaga
     4201 ctgaccggca aactggttga aaaaaaccct ccgcaaatcg tcatcgatgt caacggtgtc
     4261 ggctatgagg ccgacgtatc gatgcagacc ttctacaacc tgccgcccgt gggtgaaagc
     4321 gtacaactgt ttacccagct tatcattcgg gaagacgcac atcttttatt tggttttgcc
     4381 actgcggaag aacgcaaaac attccgccaa ctgattaagg tcagcggcat cggcgcgaaa
     4441 acggctttgg gcattttgtc ggcgatgacg gcagacgagc tggcgcaggc ggttgcagaa
     4501 gaagatgtca aacgcctctc ctccgccccg ggaatcggca aaaaaaccgc cgaacgcatg
     4561 gttttggaac tgcgcggcaa gctggtcgcg catgcggtaa cggacgggct gtttgccgcc
     4621 gcacccgccg ccgacgaaac ggaagacatc gtcggcacgc tcctcgccct cggttacagc
     4681 gaacgcgagg caaaagcggc ggtcaaaggc gttccgaaag ggacggacgt gggcgaaggc
     4741 gtgcgccttg ctttgaaaaa cctgctgaaa taatgccgtc tgaaggcggc gcgacgtttg
     4801 ccctgacgaa accctgcgtt tccgcctcgc gctgtcttcc cgacggcttt tcccgtaaaa
     4861 tggcgttatt gtccttcctt tcagacggca ttgtgtttga tgccgtctga aactgtttta
     4921 ccgaaatcga aaacaatgtt gaacaaaata ttttcctggt tcgagtcccg aatcgaccct
     4981 tatcccgaag ccgccccgaa aacgccagaa aaaggcttgt ggcggtttgt ctggagcagc
     5041 atggccggcg tgcggaaatg gatagccgcc ctggctgcgc tgaccgccgg catcggcatt
     5101 atggaagccc tggtttttca atttatgggc aaaatcgtgg agtggctcgg caaatacgcg
     5161 cccgccgaac tgtttgccga aaaaggttgg gaactggcgg caatggcggc gatgatggtg
     5221 ttttcggtcg tgtgggcgta tgccgcgtcc aacgtgcgcc tgcaaaccct tcagggcgtg
     5281 ttccctatgc gcctgcgctg gaacttccac cgcctgatgc tgaaccaaag cctcggtttt
     5341 tatcaggacg aatttgccgg acgcgtgtcc gccaaagtca tgcagaccgc gctggcgttg
     5401 cgcgacgcgg tgatgacggt tgccgatatg gtcgtttatg tgtcggtgta tttcattacc
     5461 tccggcgtga ttctcgcctc gctctactca tggctgctgc tgccctttat cggctggatt
     5521 gtcggtttcg cttcggtgat gcgcctgctg attcccaaat tggggcaaac cgccgcatgg
     5581 caggcggatg cccgctcgct gatgaccggc cgcattaccg atgcctattc caatatcgcc
     5641 accgtcaaac tcttctccca cggcgcgcgt gaagccgcct atgccaagca gtcgatggaa
     5701 gaatttatgg ttacggtgcg cgcccaaatg cggctggcga cgctgctgca ttcgtgcagc
     5761 ttcatcgtca acacctccct gaccctctcc accgccgcac tgggcatctg gctctggcac
     5821 aacgggcagg tcggcgtggg cgcggttgct acagccaccg ccatggcgtt gcgcgtcaac
     5881 ggtttgtcgc aatacattat gtgggaatcc gcgcggctgt ttgaaaacat cggcaccgtc
     5941 ggcgacggca tggcaaccct gtccaaaccg cacaccatcc tcgacaagcc ccaagccctg
     6001 ccgctgaatg tgccgcaagg cgcaatcaaa ttcgaacaca tcgatttctg ctacgaagcc
     6061 ggcaaaccgc tgctcaacgg cttcaacctc accatccgcc cgggcgaaaa agtcggcttg
     6121 atcggacgca gcggcgcggg caaatccacc atcgtcaacc tgcttttgcg cttctacgaa
     6181 ccgcaaagcg gcacggtttc gatcgacggg caggacataa gcggcatcac ccaagaatct
     6241 ttacgcgccc aaatcggttt ggtcacgcaa gatacctcgc tgctgcaccg ttccgtgcgc
     6301 gacaacatta tttacggccg ccccgacgcg accgatgccg aaatggtttc cgccgccgaa
     6361 cgcgccgaag ccgccggctt catccccgaa ctttccgatg ccaaagggcg gcgcggctat
     6421 gacgcgcacg tcggcgaacg cggcgtgaaa ctctccggcg ggcaacgcca gcgcatcgcc
     6481 attgcccgcg tgatgctcaa agacgcgccc atcctgctgc tcgacgaagc caccagcgcg
     6541 ctcgattccg aagtcgaagc cgccatccag gaaagcctcg acaaaatgat ggacggcaaa
     6601 accgtcatcg ccatcgccca ccgcctctcc accatcgccg caatggacag gctcgtcgtc
     6661 ctcgacaaag gccgcatcat cgaagaaggc acacacgccg aactcctcga aaaacgcggg
     6721 ctttacgcca aactctgggc gcaccaaagc ggcggcttcc tcaacgaaca cgtcgagtgg
     6781 cagcacgact gaaccgatgc cgtccgaaca cccgttttca gacggcattt ccacacccaa
     6841 ccccaaagaa accatgaacg acaccgccca aattaccgcc agctacggcc gccgctacat
     6901 cgtccgcacg cccgacggca caacctacga agccagcacg cgcaaaaaac gcgtcgattt
     6961 cgcctgcggc gaccgtgtcc gcatcagccc cgtcaacgcc gaacaagttg tgattgaaga
     7021 ttttttaccg cgccaaagcc tgctctaccg ccaagacgcg tggaaaacca aactcatcgc
     7081 cgccaacgtt acccaactcc tcatcgtaac cgccgccgtc ccgagtccga gcatgcggct
     7141 gctgcaacgc gccctgcttg ccgccgaagc cgccggcatc cgcgccgtca tcgtcctgaa
     7201 caaagccgac ctgcccgaaa ccgccctttg gcgcgaaaaa ctcaaattct acgaaacgct
     7261 gggttatccc gtcatcgaaa cccgcgcact ggaaaacgcc ggcagcctgc gccccgccct
     7321 gcaagggcac agcaacatcc tgctcgggca gagcggtatg ggcaaatcca ccctgaccaa
     7381 cgcccttttg ggcagccaaa ccgcccgcac cggcgacatt tccgccgcac tcgactcggg
     7441 aaaacacacc accacccacg cccggcttta tgatttgaac ggcgaaaccc aactcatcga
     7501 ctccccgggt ttgcaagaat ttggtttaca ccacctccaa gccgccgatt tgccgcgcta
     7561 tttccccgat ttccgccacc ttgtcgggca atgccgcttc cacaactgca cccaccgcgc
     7621 cgaacccggc tgcgccttca aagccgccgc gcaaaccggg gcggcaagcc ccgaacgcct
     7681 cgcctttttg cagggcatca ccgacgaact gcccgggtaa cgccttgccg cctgggcgga
     7741 aaaaatgccg tctgaagccg gattcgggtt tcagacggca tccgtttttc aaaaatgcta
     7801 caatccgctt tttactggaa catccgaaac tatgttccaa cacgtaggac gacacttaaa
     7861 gcaccgccct atgtgtcgtc ctgatttgga agggcttaca cccctcccaa ataaagtctg
     7921 attctaccgc cccgaaggac agatgtccaa gtggcggggt ttcaaccgaa aaggaaacac
     7981 gatgaagaaa aacgccccga aatcgtttga agaagccttg tcgcgccttg aatcgctgac
     8041 acaagctatg cagggcgaaa tgcccttgga agacgcgctt gccgcctatc aggaaggcaa
     8101 cgagctggtc agatactgcc aaaccaagct ggcgcaagtc gaacagaaat tacaggtttt
     8161 ggacgcggac gggccgaagg agttgaacct tgaatccgac gaatgatttg aaagcgtggc
     8221 aacagagggc gcaggcgcaa acagagctgc tgctggaacg ctttttgccg tctgaaaacg
     8281 aaataccgca cacactgcac gaagccatgc gttatgcggt tttggatggg ggcaaacgtc
     8341 tgcggccgat gctggttctg gcagcttcgg aattaggcga agccgtgcat gaagcagtag
     8401 aacaggcaat ggcggcaatc gaaatgatcc atgcctattc gttggttcac gacgatatgc
     8461 cggcaatgga caacgacagc ctgcgtcggg gcaaaccgac ctgccacatc aaatatggcg
     8521 aagccaccgc gctactggtc ggcgacgctt tgcaaaccca agccttcgac gtgttaagcc
     8581 gtccgacaga actgcccgcc gcacgccagt tggcaatgtt gtcggtgttg gcgaaagcgg
     8641 gcggcagcgc gggcatggcg ggcggtcagg caatcgattt ggcgaatgtc ggcaaacaaa
     8701 tggttcaagc cgatttggaa cagatgcaca gcctgaaaac gggtgcgcta atccgtgcgg
     8761 cggttttatt gggggcgatg tcctgcccga atctgtctga agacagtctg gcggtattgg
     8821 acgattatgc ggcaaaactg gggctggcgt tccaagtcat tgacgatgtg ttggattgtg
     8881 aagcggacac ggcgacttta ggcaaaacgg cgggcaaaga cgcagacaac gacaagccga
     8941 cttatgtgaa actgatgggc ttggaagcgg cgcgctcata cgcacacaaa ctggttgccg
     9001 aagcggtcgc gctgctcgaa cccttcggcg acaaagccct gcgcctgcgg cagttggcag
     9061 aatttgcagt cgcccgcaaa tattaaaacc ggcgtatatc gccggctgaa acgtgccggc
     9121 acagcgatgc cggcctgcac ttgaatgaaa caaatgccgt ctgaacaggt ttcagacggc
     9181 attcctatgc ctgcctattc cctgttgcgc gtgtgccaaa aataccgcca cacgaaacag
     9241 ggaaacgcaa aaatcaggta caggcagacg acggtggcgt agaactccca accctgatcg
     9301 tgtaccgctc ccgcacggga ttcgaggatg taggcaagaa cggcggtcag cgcgaaacct
     9361 gccgccagct cgatcaagtg gtgcccgaaa tgtttgcgct tgagcggtac gatgccgaac
     9421 agcctggtcg tgaggaaggg ggcgttggca aaaatcaagg caagcagcaa aaggatgtac
     9481 atggatgcgg tcatggcggg ctttctgttt gggcattgtc ggcggcgatg ccgtctgaac
     9541 tgcgttgcgg gacggcatat cttaacaaaa acggcagcct ctgacacggc tgccgttgcg
     9601 gcggcttaca gcgtgttttc caacgccttg gcgcaccagt cgataacggt ttgcggcata
     9661 atgccccaca gcaccaacag cagcgcgttg acgctcaaaa ggaattttgc ggcattgttg
     9721 ccgccgacgg tgggatgggc gcggccggat tcgtcaaaat acatgacttt gaccacgcgc
     9781 aggtagtaga acgcgccaat cagcgacatc acgacggcaa atacggacaa ccaaacatag
     9841 ccttgtttta agagtgccat aatcacgcca aatttggcgt aaaaacccat cagcggcgga
     9901 atgcccgcca tagagaacat aaccagtaac atcaaaaagg caagccatac gcggtgttgg
     9961 ttcagccctg ccaaatcgct gatgttttcg cactcgttgt ccccatccga caacaccatc
    10021 aacacgccga accctgccgc cgccatcagc gcgtaggtaa tggcgtaata gagacccgcc
    10081 gcaaagccga ccgcgcccgc cataaacgcc agcaggataa accccatatg cgatacggtg
    10141 gaataggcga gcatacgttt gatattggtc tgcatgatgg cggcaaggtt gccgaccagc
    10201 agcgaggcgg cggcaagcag ggcaaacatc agagaccagt catgatgcac ggttcccaac
    10261 ccggtaacga ggatgcggaa agcgaaaacg acggcggcga ttttcggggc agtgccgact
    10321 agggcggtaa cagaagtggg cgcaccgtga tacacgtcgg gcacccacat atggaacggc
    10381 accgcgccga gtttgaacgc aacggcgacg acgataaaca ccagacccag tttcaacagc
    10441 cattcgtttg cttcttcatt gaaggaagag gcgagcacgc cggcaaattc cagcgaaccg
    10501 gttgcgccgt aaaccataga aataccgtag agtagcagac cggatgccag cgcgcccaga
    10561 acaaaatatt tcaaggcggc ttcggcggca aagccggaat cgcggcgcag ggcaatcagg
    10621 gcgtaaaggg caagcgacaa gagttccaaa ccgatatagg cagttaaaaa atgccccgcg
    10681 ctcaccatca cgctcatgcc caagagggca aacagcgaca gggtgtaaaa ctcgccttta
    10741 aaaataccgc gcacttggtt gtagggcttg gcatagacaa acagggtaaa catcaaggca
    10801 tataaaacca tttttgccaa acgcgacata ccgtccgcaa tatacatccc gttgaacgaa
    10861 gacgtgctgc cctgttccca cacggcaagc tgcaccgccg ccgtaaccgc caccgttgcc
    10921 aacgcgccgt aatgcgtcca acggcgtttg tcatcactga cccacaagtc tgccagcaac
    10981 aataacacca gcagcgacag cagcgcgact tcgggcagtg cgggcatcaa attcaaatca
    11041 gaccaattca tttacacacc tcacatcttg ctttgcgcca cttgggcaat caaatcgttt
    11101 gccgcctgat gcaccacttc gataaatgcg ttcggataca ggcccatacc caataccgca
    11161 accgccaaaa ccgccagaat cgtaaactcg cgacagttga tgtctttcat ttcggcaacg
    11221 tgcggattgt gaatcgcgcc gaaaatcacg cgcttgtaca tccacagggt ataagacgca
    11281 ccgtaaatca gcgtcatagc cgccaacgcg cccacccaga aattcacttt aaccgcgccc
    11341 ataatcacca taaactcgcc cacgaagcca gaagtcgccg gcagaccggc attggccata
    11401 ccgaacaaca tcataaacgc cgcaaactta ggcatcacat tgaccacgcc gccatagtca
    11461 gcaatattgc gggtatgcag gcggtcgtac atcacgccga tacacataaa catcgccgcc
    11521 gacacgaaac cgtgcgaaat catttgaatg attgcgcctt tcaacgccca gtcgtccaac
    11581 tgcccgtcaa caaacaaaaa catcccaagc gttacaaaac ccatatggct gatagacgaa
    11641 tacgccacca gttttttcat atcggtttgc accaaagcca ccataccgat ataaatcacg
    11701 gcaatcaggc tcaatacgat gatcacaggg gcaaaatagc gtgccgcatc cggcataatc
    11761 ggcaggataa aacgcaagaa accatacgca cccagtttca gcgtaattgc cgccaacacc
    11821 atcgaaccgc cggtcggcgc ttcaacgtgg gcatccggca accaagtgtg cacagggaac
    11881 atcggcactt ttacggcaaa tgacaggaag aatgccacaa acaaaagctg ttgtacgccc
    11941 aatggaatct gcttgatgtt ttggaaatcg acaatagaga agctgcctgt ctgatagtac
    12001 aggtaaacca tggcaaccag catcaaaagc gaacccatca gcgtatagag gaacagcttg
    12061 accgacgcat aaacgcggcg cgggccaccc catacaccga taatcaggta cagcggaatc
    12121 agcataccct cgaagaacac ataaaacaga atcgcatcct gtgcggcaaa cgcgccgtta
    12181 atcaaacccg acatcatcag gaatgccgcc atatactgcg ccggacgttt ctgaataact
    12241 tcccaaccgg ccaataccac caacagcgta ataaacgcat tcaagatgat aaagagcact
    12301 gaaataccgt ccacgcccaa tgcatagttg attttcagca gcggaatcca ctcgtggaac
    12361 tcggtaaatt gatagccgcc gctcaaacgg tcgaaaccgg taaacagggg cagcgttacc
    12421 aagaaaccgg caagcgcacc catgaaggca agcacacgcg ccagcggcgc acggctgtcc
    12481 gaccccgttg ccaaaaccag cacacctgcg gcgatgggta tccatattgc caagctgagt
    12541 aggtagttgg aaaacatagt ggttaacctg tggttaaaat aaaaatatcg ttgtacgggt
    12601 tggtttgtca gacgacgttt taagagaccg tctgaagcct catccttatc tgaacaatcc
    12661 ccagaaggtc atgccgagca gtaccaatac gccgaacacc atagcggcgg cgtaggtgta
    12721 gataaagccg gtttgggctt tacgcacttg cgcggcaatc gcgccgacca gtttggcaga
    12781 gccgttgacg atgccgttat cgataatggc ggtatcgccg actttccaga agaaggtacc
    12841 cagtgcgcgc gtgcctttag cgaagacgtt gaaatacagg gcgtcgaggt agtatttgtt
    12901 ttcaaacaaa acgtaaaccg gacggaacgc ctgtgcaatt ttcgcaggca ggtgcggcag
    12961 tttgacgtac aaaagccatg cgctcaatac acctgcgata gcgaggtaga gtacaggaga
    13021 atgcaggctg tgcgacacca ttgccaatgc gccgtggaac tcttccttca tgatgtgcat
    13081 ggtcggatgc gcgtcggtat tgacgaaaat cacgtctttg aagaaatcgc cgtaaagcat
    13141 aggcccgatg gcaacatagc cgatgatgac ggacggaatg gcaagcaaaa tcaaaggcag
    13201 ggtaacgaac agcgggcttt cgtgcggatt gtcgtttttg cccaagccgt gatgttcttc
    13261 gccatgtccg tctgaatggt gttcgggcag gctgcgccat ttctcttcgc cgtggaacac
    13321 cataaagtat tggcggaacg cgtaaaacgc ggtaacgaac acgctggcaa ggacggcaaa
    13381 ataggcaaag ccgctgccgg gaagggtgct gtatttcgcc gcttcgataa tcgaatcttt
    13441 ggagtaaaag ccggagaaga acggcgtacc aatcagcgac aagttaccga tcaacatggt
    13501 caaccaagta atcggcatgt attttttcag gttgcccatg tggcgcatgt cttggtcgtg
    13561 atgcataccg ataatcgcgc tacctgcggc caagaacaac aaggctttaa agaaagcgtg
    13621 ggtcatcaca tggaacatcg ccacggaata ggcagacgcg cccagagcca cagtcatata
    13681 gcccagttgc gacagggtgg aatacgcaac cacgcgcttg atgtcgtttt gaatcacgcc
    13741 caagaaaccc ataaacaggg cggtaatcgc accgattacc atgatgacag acagcgcagt
    13801 gctgctcatc tcataaatcg gcgacatacg cgataccatg aacagaccgg cggtaaccat
    13861 ggttgcggcg tgaatcaatg cagaaatcgg ggtcgggcct tccatcgaat caggcagcca
    13921 gacgtgcagc gggaattgtg ccgatttacc catcgcaccg ataaacagga gcaaacaggt
    13981 tacagtaatc aaagaccatt ccacactggg gaacaattga atggtggccg tctgaacatt
    14041 aggcagataa gcgaatacat cttgatagcg caggctgccg ccgaaatagg caagcaccaa
    14101 gccgataccg agcaaaaagc cgaagtcgcc gacacggttg atcaaaaagg ctttcaggtt
    14161 ggcaaatgtc gcgctcggac gtttgaaata gaaaccgatc aagagatacg acaccaagcc
    14221 caccgcttcc caaccgaaga agagctggat gaagttgttg ctcataatca gcatcaacat
    14281 actgaaagta aacaaagaaa tatagctgaa gaagcgttgg taaccgactt tttcatcgtg
    14341 catatagccg atggtgtaga tatgcaccat caacgacacg cccgttacca cgaccatcat
    14401 catcgccgtc atcgtatcga ccaagaagcc gacggaaaaa tccaagccgc ccattgtcag
    14461 ccaggtataa acgttctcgt caaacttggc acggctgccg tcaataaagc cccacaacac
    14521 ataagccgac agcacggcag acaccgccac accgagtatc gtaaccgtat gcgcgccggc
    14581 acgtccgatt ttgttgccga ataaacccgc aatcagcgag cctgccaaag gcacgagggc
    14641 gatgatgaga tataaagtca tgtcgttcat ttaaatttgc accagtaaaa tataaatgct
    14701 gattgcgatt aaactaaaaa tcgtaccata cgcaatcata gaagccgaac gccctgtttt
    14761 caaatatgcc gtttccaaca caataatatt ggcagcaggc ggcagcaacg gtattaaata
    14821 caatgttttt tggttttcaa gaatcaactt caagtcaaaa tatgcagcta tttggataaa
    14881 aagaaaaaca atccctataa ataaaatgtt t
//
LOCUS       DAWFAN010000035        14889 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWFAN010000035 DAWFAN010000000
VERSION     DAWFAN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.035"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(141..836)
                     /locus_tag="WGO65_000765"
     CDS             complement(141..836)
                     /locus_tag="WGO65_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0327085.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(1007..1666)
                     /locus_tag="WGO65_000766"
     CDS             complement(1007..1666)
                     /locus_tag="WGO65_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ0327086.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(1864..3975)
                     /locus_tag="WGO65_000767"
     CDS             complement(1864..3975)
                     /locus_tag="WGO65_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0327087.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGYSRAFTASIDPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GNSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSIDTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDPKNNPYIYAVSGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYDSRNKEVTTLPGFARVDAMLGWNHKNVNVTFAAANLFNQKY
                     WRSDSMPGNPRGYTARVNYRF"
     gene            complement(4161..4766)
                     /locus_tag="WGO65_000768"
     CDS             complement(4161..4766)
                     /locus_tag="WGO65_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0327088.1"
                     /translation="MTRQSLQQAAESRRSVYLLNKNLPIGKDEIVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLKLFKAGAATILFYED
                     QNVVKGLQEQFPAYASNFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(4816..5214)
                     /locus_tag="WGO65_000769"
     CDS             complement(4816..5214)
                     /locus_tag="WGO65_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0327089.1"
                     /translation="MSNCCNRIQPVLLSVLRIVTAYLFLLHGTSKFFAFPIEMGSGSP
                     GGLMLLAGILEVVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFFYIAAAGGGAWSLDRLFFKRKA"
     gene            5322..6233
                     /locus_tag="WGO65_000770"
     CDS             5322..6233
                     /locus_tag="WGO65_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0327090.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLIRNGVPKTPEEVTRLPAVLPTYTNQQKLDITHKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAVEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            6223..7524
                     /locus_tag="WGO65_000771"
     CDS             6223..7524
                     /locus_tag="WGO65_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ0327091.1"
                     /translation="MPSETARPLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPNLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDSPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(7592..7816)
                     /locus_tag="WGO65_000772"
     CDS             complement(7592..7816)
                     /locus_tag="WGO65_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327092.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVAAWVAVIWAYLMIESEKNGRY"
     gene            7919..10069
                     /gene="dinG"
                     /locus_tag="WGO65_000773"
     CDS             7919..10069
                     /gene="dinG"
                     /locus_tag="WGO65_000773"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ0327093.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            10105..10719
                     /locus_tag="WGO65_000774"
     CDS             10105..10719
                     /locus_tag="WGO65_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ0327094.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGAPVAGAASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRASVTQSDFQPAGETEETYSRTILRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAGWLAAEYRLPASIEDIAASVGNDEILAA
                     EAYLAARLVCADLSRKETVFLARLSQALELDDNLVENLERQLGF"
     gene            complement(10824..12692)
                     /locus_tag="WGO65_000775"
     CDS             complement(10824..12692)
                     /locus_tag="WGO65_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0327095.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILAEIRDGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSANENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTASSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTENGKQQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFISFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLVEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRIGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSHGGALQ"
     gene            complement(12772..14142)
                     /gene="purB"
                     /locus_tag="WGO65_000776"
     CDS             complement(12772..14142)
                     /gene="purB"
                     /locus_tag="WGO65_000776"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ0327096.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAASEFIHFACTSEDINNLSHALMLQEARETVILPKLAEIIGKLTDMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANIVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPVSRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKVFIESLEIPTEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(14214..14846)
                     /locus_tag="WGO65_000777"
     CDS             complement(14214..14846)
                     /locus_tag="WGO65_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ0327097.1"
                     /translation="MENVNRVPEQARYDAERRQADEVLAGVFPAVSIFGSARTPQNHA
                     DYTFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYIVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         3564 a         4127 c         3821 g         3377 t
ORIGIN      
        1 gtactggttt ttgttaatcc gctatatatt ctgatttaaa accataaggc tttaagcaat
       61 catctcttct ataaagccta aatacaaaag gccgtctgaa atcctatttt cagatggcct
      121 ttactcattc aatcctcaac ttattgcggc tttttctgct cttcgcgtac tttatccacc
      181 aactggtcaa tcgtggtcat accggactgc cagtctttaa attcaacttg gtatttgccg
      241 ccgacaatca cagtcggtgt gccgctgatt tggaatttat tggtcaactc ttccatttga
      301 gccgcacgcg cttggctttc aggagcctca aatgcagcca atactttttt gccgtcaaac
      361 gctgtttgct cggacagcca ttttttcagg gtatcggtat cggccagatt gattttttga
      421 ttaaccatcg catcgaaaat atggctgttg gctttatctg attcaccggc catttccact
      481 gcggccgcca aacgtgccaa aggtttcatt tcatcacccc acacgacatg ctcccggcgc
      541 atataggtat cgtctttaaa cgttttgatg tgctcgctca agaccggctc aagatgggcg
      601 caatgcgggc agaagtagcc gaaaaattcc aatacttcga ttttaccggc ctgctgttgc
      661 ggaataggcg tagacaatac agtgtagttc acaccttcgt tcaactcagc aggggctgcc
      721 ggagcagatg agctgctttg gacgctgtct gccggaacgc tggtttcagc ctgtttgcta
      781 caagcggcca atgccaacag ggtcaatgaa gtcaaagcta aggttttcag tttcataggt
      841 atctcttgtg tgtcagatta atgtgcggat tttatgacat tttattgaag gcgtgttcga
      901 tttgagtgaa aaaatgtgtt tagttttatt tcagccttta taaaggccgt ctgaaatatg
      961 gcttagataa ttagccttat tttcagacgg cctttgttct agtaatttac ggtttcggac
     1021 attttgtctt caatccgtcg atgtgttcca ttaagtcagg tgggacatga ttccacatag
     1081 gatcaagaat aaaatcgata ccttcccgtc tggctaattt ggctgctggg acaaaatcgg
     1141 aatcaccaga aaacaagatg attttatcgg cttgtttttt taaggtaata gatgaaatat
     1201 ccaagcctat acgcatatct acaccttttt gtttaacatc gagcacgaca tcgttttccg
     1261 taatttgagt tgacgggttt ttcagaagat ttttcatgac ttggggtttt aatatccatg
     1321 ctgtattgtc cgacagcttg cctaaacgta acgctaattt acgcttcttc ttcaattcgg
     1381 tatgcagatt gcaacgaaat atggcttccg gagtctttga aaaatctatg gcacgatgag
     1441 atacgggatt gtgcattttc ttttcaaacg gcacgcagtc gtaaaagaaa attctatata
     1501 aatcatccat ttgatctgtg ccgttgattt tatctttcag atgcagcatc gaatatcgga
     1561 aagccaaatc agcaattctt tttgcatcgt gtggattttg agacaggcct aatgaccgta
     1621 cccgcttaat aaaaaatgca ccgtcaatca aaatggcggt tttcatattg cttcccctat
     1681 atttgtcaaa gatataaaaa agcccttggg atcggcatac ccgctatgat ttaatgggta
     1741 atgcgtactg ccaagggcac tatttctttt tggattatac tgatgtagat aaagcctgtc
     1801 aaaaagttcg tatgctgaat ttacggatta tcaggatttt cagacggcct ttgcctgatt
     1861 tcatcagaaa cggtaattta cccgggcagt atagccgcgc ggattacccg gcatagagtc
     1921 cgaacgccaa tatttttgat tgaacagatt ggctgcggca aaggtaacgt taacattttt
     1981 atggttccag ccaagcatgg catcaactcg ggcaaagcct ggaagcgtag tcacttcttt
     2041 atttcttgag tcgtaaccgt agcgtttgcc tgtaccggtt acgccgattt cgccgtagag
     2101 gttttcggtc ggtgtataac ggaaaaacag gttgccggta acgttgctgg tattattcaa
     2161 atggatgccc actcggtcgg gattttcttt atcttcaacg actttcgcct gcatcacgcc
     2221 caacgaaccg cgcagataga gttttttagg gatgatttgc ccgatggcgg acaattccac
     2281 gccgcgcgaa cggtgtttgc cgctaaccgc ataaatataa gggttgtttt ttggatcggg
     2341 gcggtagcgg atattgaagc gttcgatttg gtaggcagac aacgtagtgc tgaggcggtc
     2401 gtccagccaa ctgcttttca caccggtttc gtattggcgg gtgtactcgg ggtcggcgtt
     2461 gaacacggcg gaagacgacg tatcgatgct caaatagccg ccgcgtccgc cataaggcgc
     2521 gaagcctttg ttatacgagg cgtaaagtgt gtggactggg ttgatgttcc acactgcgcc
     2581 gatgttgggg ctgaacgagt gtccgctgta ttggcggctg ttgccggtga gtttgttttc
     2641 ggaattaaag gtgtatttgt cgtaacgacc gccgaggacg aatttcaagt cgggcgtggc
     2701 ggagaagatg ttttgcacaa agatgccgta ggagtcggct ttgtggcggt tttgggtgag
     2761 gataggctgc aatctgcccg aagccggcca gcttgctcgg tcgtatggat cgatggaagc
     2821 agtaaaggcg cggctgtaac ctaaggtcgg gttgcggtgt tcgcggctgt aatccatgcc
     2881 tacggtcagg tggttttcaa aacggccgat ggtgtagtcg ccgtttagcg tgaaattgga
     2941 cgacagggtt ttgttgtcgg tctgctgcca ggcgtagtta cgtttgatta agttgccatt
     3001 ttcgctgcct gcatagaaat gatcaaaatc ctgcgccgcc gtgcggtggg cgagctgcca
     3061 ttgggcacgc catttgtcgt tgaaggcgta ttcaaggtcg gaacgccaaa cttgcagctt
     3121 gtctttgaca aaatcgttcc ggtgggcgaa ccccatgcgg taaggcagtc cgaagcggtc
     3181 gtacacggac ttggtcggac tgcggtcggg cgtgcgctcc acattgtcgt aggtgtattg
     3241 ccccgtccac ttcaagccgt tgtcgagttt gacggtaatg ctgggcgaaa ccatgacatt
     3301 tttgctgtct atgccgctgc ggaacgaatt ggcgcgcccg acttcgccgg tgagacggat
     3361 ggcgacgttt ttgttcagca cttcgttaat gtccatattc aggctgcggt ttgcccacga
     3421 accgtaaacc gcaccgatgt tgcggctttg tttgaagttg gcgtatttgc tgaccatgtt
     3481 gatgacgccg ccgccgttgg tgcggccgta aagcacggaa gacgggcctt tcaggatttc
     3541 cacgcgctcg atgttggcag tgctgcggcg gacctgcccg ctttcgcgca cgccgtcacg
     3601 gtaaatatcg gatgcgtcag cttgaaagcc gcgcaggaaa atgctttcgc cgcgcatatc
     3661 gtaggcagcg tcgatgccgg cattgccttc gaggatggaa ctcaaatcgt tcgtaccgta
     3721 atttttgttt ttctggatat tgagcgtatc gatggtttgc ggcgtttctt tgatgagctg
     3781 tccgttgcgg gtaacggcgg cttcgtcgta gttgatgtag cctttgagta cgctggtgtc
     3841 ggactgtccg accacggaaa cggtaggtag cgtggcggtg taatgttcac cattgtcctg
     3901 cgtatcggcg gcagcaacag ggaaggaagc aataatcagc gtgggtaata aagctaaatg
     3961 aaatgatatt ttcattttta tactcaattt aacaaaacaa ccgaattata ttgcctcacg
     4021 gaggaaatga gaataatttc ttttaactat attgaacatg atttttgtaa acaaaggtct
     4081 cagaatgcgg aaaactcgcc gcctgatact gaaaaatgcc gtctgaacag ggttcagacg
     4141 gcattttttt gaccgcgaaa ttatgcgccg aacactttca aacgttctgc aactggttca
     4201 aaggtctttt cacctgccgc cccttcaata ccgccaataa ccatttgcgc gcgcaacaac
     4261 cagttttcgg ggatattcca cgctttggca atcgccgcat cgggcaaggg attgtaatgt
     4321 tgcaggtttg cacctacgcc gaccgcggca agtgtcgtcc aaacggcata ctgcaccatc
     4381 gcgttcgcct ggtccgccca aacgggaaag ttggaggcat aagcagggaa ctgctcctgc
     4441 aaacctttga caacattttg atcttcataa aacaaaatag ttgccgcacc cgccttaaac
     4501 agcttcaatt tttgcgcggt cggttcaaaa ctgtcggcag gcacgacggc acgcagcgcg
     4561 tcttcgacaa attgccacac cttatcatgc tcttcgccaa acagcacgac cacgcgggca
     4621 gattgggaat taaacgaaga aggcgtgtgc aaaacggcgt gttcgacgat ttggacgatt
     4681 tcatctttgc cgatgggcag atttttattt agcaaataaa cggaacggcg gctttccgca
     4741 gcctgttgca gggattgacg ggtcatttct tttcctttca aaaatattcg caaaatacgc
     4801 ttcagacggc atagttcagg ctttacgctt gaaaaacagc ctgtccagcg accacgcgcc
     4861 gccgcccgcc gccgcgatat agaagaatac gaagcagaac agcaccgcgg actcgccgcc
     4921 gttggcaatc ggaaacaaag catttccgga agcgtgcgcc ataaaatagg caaccgccat
     4981 ctggccggac aaaacaaacg cggcagggcg cgcaaacagg cccaacacca gcaaaatgcc
     5041 gccgacgact tccaaaatac cggcaagcag catcagcccg ccgggcgaac cactgcccat
     5101 ttcaatgggg aaggcgaaga attttgacgt accgtgcaac aaaaataggt aggcggttac
     5161 gatacgcaaa acagaaagca gaacaggttg gatacggttg cagcaattgg acataaaagc
     5221 tccttcggta tttatcttca tttcaggcgg cagagtatag atgtttcccc ttttcaaata
     5281 tatactgctt ttattgatac aacttttcca cacaagaaaa tatggacacc ctgttcagcc
     5341 tcaaagtttt ccgccaagtc gtccaaagcg gcggctttac ccgcgccgcc gacgcgctcg
     5401 gcatctccac cgcaatggca agcaaacacg tcagccactt ggaaaacacc gtccaagcca
     5461 aactcctgca ccgcaacagc cgcaatctca gcctgaccga agcgggggaa gaatactacc
     5521 gacaatgcag ttacgcgctc gacacgctcg acgatgccgc gcaaaaagcc gcagggggga
     5581 cggaaaaacc gcaggggctg ctgcgcgtaa cgatgccgct gtggtttgcc ggcagccaga
     5641 tatgcaactg gctggcggaa taccgcgaac gttatcccga agtaacatta gaactgattt
     5701 tggacaaccg gcacgtcgat ttgattgccg aaggcgtgga tttggcgttg cgcgtttccc
     5761 aaaccctgtc cccgtcgctg attgcgcgcc cgctggcgga aatcgaattt gccctgctcg
     5821 cctcgcccga cttcctgata cgcaacggcg taccgaaaac gccggaagaa gtaacgaggc
     5881 tgcccgccgt cctgccgacc tacaccaatc agcagaaact cgacatcacc cacaaatcgg
     5941 acggcaaaaa ataccggctt gagctgaccc ccgtcatccg taccgacaac acgctgatga
     6001 tgcgcgaaat gattaaggcg ggcgcgtgca tcggttatca gccgctttgg gcggtggaac
     6061 acgatttgcg ctgcggcacg ctggtgaggc tgctgcccgg atacgccgtc ccgaccgacc
     6121 ggctgaatgc cgtttatgca gacagggcat tcttaagcgc gaaagtccgc agcttcatcg
     6181 attttctgaa cgaaaaaatc gccagcagga aaggctgccg aaatgccgtc tgaaaccgcc
     6241 cgcccccttt atgcggacac gcgcgccgca cacacgctcg tctggttccg ccaaaacctc
     6301 cgcatccgcg acaacgccgc cttatgcgcc gccgttgccg aaggtttgcc cgttatcggt
     6361 atttggattg acgatgccga aacagacaac cctcgccgcg ccgcgttcta ccgccaatcc
     6421 gccgccgaac tcgcccaagg gcttgcaggg cgcggcatcc cgctctacac ggcggcatcg
     6481 cctgccgagc tcgtccggct cgccgtccgc ctcaatatcc gcgccgtcat cgccgacgaa
     6541 tcccatactt ttgccgacaa actcgccgac aacgcccttt ggcatgaatt ggacaaacac
     6601 ggcatcgcgt taaccttcgt caatgaccgc gccgttttcg gcaaaaccga cctgataccc
     6661 gacggcggca cggcacacac cgatttcgac cgctaccgcg aagtatggct cgaccgcttt
     6721 tccaagcagc cccccgccgg tccgaaccta ttcgcggcat accgccaacc cttccccgaa
     6781 aacctttccg ccccgccgcc tgccgccctt tcagacggca tcttcctgcc gcaaaacagc
     6841 ggcgaaacgg cggcttggcg gcagtggcgg cggtttctcg aacaggcgga ttcctactcc
     6901 gttttaaagg atttcccctc gcgcaaacac acttcgctga tgggcgcgta tttgagtgcc
     6961 ggctgcatct cgccgcgcct gctcgcgcgg gaaagcctcg aacgccgtct gaacgcgtgg
     7021 gcggacaaca tcatccgccg cgattttttc cttcaacttg ccttgcagca cacggatgac
     7081 agcccttcag acggcaatcc ggaacacacc ctgcgcctga cgctttggca gcagggccag
     7141 accggcattc cgattatcga tgccgcgatg cgctgtttgc acaaaaccgg cagcctccac
     7201 cccgccctga gacgcttgag cgcggatttt ttctgccacg ttttaaacct cccccgccgc
     7261 gaaggcgaga tatggtttgc ccggcagctg accgatttcg atgcggcaat caaccaaggc
     7321 aactggtggc ttgccgcctc acggcacacc tgccccgaca ttgccgccgc ctcataccgt
     7381 accgacccgg acggtacttt tatcaaacgg cacattcccg aactcgccca cctgtccgcc
     7441 gacaccgtcc acacgccttg gcggtttgcc tgttcggtcg atacccacgg ctatcccgcc
     7501 tatcctgtcg ccggtattcc ctaagcggta cggcaaccta aaacaaatgc cgtctgaact
     7561 tccgtttcag acggcattgc gtccgaatga ctcaatatct tccgtttttt tcactttcaa
     7621 tcatcaggta tgcccaaatc accgccaccc aagccgccac cgttcccatc gccggcaaaa
     7681 aatgctgcca atccgtcatc ggcggcacag ggcaacggcc gcattcgatt tccgcgccgc
     7741 ccaacaaccg gtacagcaac atatccgcaa tccaaagcaa caacgggtat ttcaaacgct
     7801 gcaaccaaaa aaccaaccac gtccgcacga ttcacctcac cccccattcc gacaacgata
     7861 ttgtaccgct tccgtgcaat gttaaaataa gcccacttct accgccgtac aaaacgccat
     7921 gctcaccgat ttagaaaaaa acgccatccg cgaccattac caaaacatcg gcaaaaacct
     7981 gcccggtttc cgtccgcgcg cttcgcagcg ggaaatgatt gcggcggttg ccaacgcttt
     8041 ttcgcggacg ttggcgcgcg aagaaggcgg cgagccgccc aagcgcgagg gcgagagcat
     8101 cgccgtaatc gaagggccga ccggcgtggg caaatcgctt gcctacctgc tggcgggcgg
     8161 catcatggcg cagacgcgcg gcaagcggct gattgtgagc agcgcaacgg ttgccttgca
     8221 ggagcagttg gtagaccgcg acctgccgtt tctggtcgaa aaaagcggtt tggaactgac
     8281 cttcgcactt gccaaagggc gcggccgcta tctctgcccc tacaaactct atcaactgac
     8341 gcaaagcaat gcccagcaaa acctgctcgg ctttgaagcc cccgccgtct tgtgggacag
     8401 caaacccaag cccgaagaat tgaagctgct gcgcgacatc gccgacgaat tttccgcccg
     8461 acggttcaac ggcgaccgcg acacttggcc ggaaaaaatc gatgacgcga tttggctcaa
     8521 agtgaccaac gaccgccacg gctgcctgaa aaccgcctgt cccaaccgtc cggaatgtcc
     8581 gttttaccta gcacgcgatg tcttggaaac cgtcgatgtc gtcgttgcca accacgatct
     8641 tctgcttgcc gacatcagta tgggcggcgg cgtgattctg cctgcgcccg aaaacagttt
     8701 ctattgcata gacgaagcgc accacctgcc caaaaaagcc ctcagccgtt ttgccgccga
     8761 acattcatgg aatattgccg tttggacgct ggaaaaactg ccgcagctga ccggcaaaat
     8821 tgccgcgctt accgataaag ccgaacttgc caacctggcc gacgaagccg ccgcatcctt
     8881 gctcgacagc ctgcacgaat ggcagttcca tttggcggaa gagccgtctt taagtatggg
     8941 gccgtctgaa aacgacagac gaaccaacag cgaaccgact tggctgtggg aagacggcaa
     9001 aatccccgaa ggcctcgaaa ccaccgtttc caatacggcc attgctgcgc gcagcctgct
     9061 caaacacgtt gtcgggctga acgatgcgct ttccgccgcg cgccgcgaaa aagaacagga
     9121 cggcgcgctc ctcgaccgcc tgaccggcga gttcggcatc ttcatcgtcc gtatcgaaca
     9181 aatcagcgcg gtttgggatt tgctctccac cgtccccctc gaaggcgaag agccgctggc
     9241 aaaatggata acccgccgtg ccgacgacaa aaacgactac attttcaacg ccagccccat
     9301 cagcagcgca tcccaccttg ccaacagcct gtggcggcgt gcggcaggcg cggtattgac
     9361 ttccgccacc ctgcaatcct tgggcaactt caacctgatg ctgcgccaaa ccgggctgca
     9421 atggctgccc gaaaccacca ccctcgccct caaaagcccc tttgactttg aaaaacaggg
     9481 cgaactctac atccccccca tatacgccag ccccaaagac cccgaagccc acaccgccgc
     9541 cgtcatcgaa tggctgccca agcttatttc gcccaccgaa gccatcggca cgctcgtctt
     9601 gttttcctcg cgcaaacaaa tgcaggatgt cgccctgcgc ctgcccggag actacctgcc
     9661 gctcttgctc gtacaaggcg aattacccaa agccgtcctc ctgcaaaaac accaccgggc
     9721 catagaagaa ggcaaagcca gcatcatctt cggactcgac agtttcgccg aaggactcga
     9781 cctgcccggc accgcctgcg tgcaagtcat catcgccaag ctgccgtttg cgatgcctga
     9841 caaccccatc gaaaaaaccc aaaaccgctg gatagaacag cgcggcggca atccttttat
     9901 tgaaatcacc gtccccgaag ccggcatcaa actcatccag gccgtcggcc gcctcatccg
     9961 caccgaacaa gactacggcc gcgtaaccat cctcgacaac cgcatcaaaa cacagcggta
    10021 cgggcagcaa ttattggccg gtctgccgcc gtttaaaagg atagggtaaa cctaccgccc
    10081 ttcccgcaac agaaaggaaa agtcatgaac ttcacccgac tgctcaacca agtcttaagc
    10141 acggttcaaa aaaaaggcaa cacattctcc gacagcccgc tcaattcatt cggcggaggc
    10201 gcgccggttg ccggtgccgc ctccatgctg ctgaacggta aaaaccgcaa aaccattacc
    10261 aaaatcggtt cgaccgccgc tttgggctac ctcgcctacc ggggctatca gatgtggcag
    10321 caaaacaaag ggcgggcatc cgttacacaa agcgatttcc aacctgccgg agaaactgaa
    10381 gaaacataca gccgcaccat attgcgcacc atgatagccg ccgccgcttc agacggcatg
    10441 atagacgaag ccgaacgccg gactatcgaa caggaaagcg gcacagaccc cgaaaccgcc
    10501 ggatggctcg ccgccgaata ccgcctgccc gcaagcatcg aagacatcgc cgcctccgtc
    10561 ggcaacgatg agatactggc ggcggaagcc tatctggcgg caagactggt atgtgccgat
    10621 ttgtcgcgga aagaaaccgt cttcctcgcc cgcctgtcgc aggctttgga actggatgac
    10681 aatctggtgg aaaacttgga aaggcaattg gggttttgat tcaaaattcc ctcccgcagg
    10741 ggagggaaaa taaaaaccgc cgccgccacg aagggcaaac ggcggcatag gcagctacag
    10801 gcaatatccg gcaacaaggc gggctactgc aatgcgccgc cgtgggaaga tttaagcagg
    10861 cgttcgtgtt tgtggaattc ccgccccata taataagaac cgaaataggt caggtggtct
    10921 tggtcgccgt aaagatagcg tccgtgtatt tcgaccgtgt ttttgggcag gtatttttgt
    10981 gcgtccaccc aatgcacatt gggaatatct ttaatcaaat caaagaccgc ctgattgctc
    11041 ttgccgatgt cgcccatagc ctgaatgggg cggagatatt ggtttgcggc aaatcttttc
    11101 aatttttcct ccctcagggg cgaacggctg attgatgtgt tgtttgcaaa aacatagacg
    11161 ggtttgacgg cggctatcct tttgacggtt tccctgaatc gggcttggaa cccgggtatt
    11221 aggaaggatt gcgcttcaaa tctcggcacg ggctggccgc ctatcctcaa atcatagaat
    11281 tgggcaatga aaacggcttc ggctttttca acttcatccc ggtattttcg acataacggg
    11341 ttgtctgcca gcttctcatc tacccaaacc aaacactccg aatcgaggga caggattttg
    11401 gctttccacc cttcccggct gccgacataa tccagaaacc cccgcaggtg tccggcgtgc
    11461 gagtcgccga gggtcaggac ggtttccgga aaatgatttt ccgcagcaag gggcgcgccg
    11521 ggcaacgggc ggaggtgttc ctgtttcaat atcccccttg cgtacaggtt gtaaccgaca
    11581 agtatcaggg acggggcgag atagaggcag aaaaatgcct ttttgaaggt catcttccgt
    11641 tttctaagcg gctgttcaac caaataataa ctcaacaggg aaaatccggc cgtcaatgcg
    11701 gcaaccgccg atacggcagg cagtccgagc tgtttgtcgc ctgtaatgta atgggcgaag
    11761 gaaataaaaa tccaatggta caggtatagg gaataagaga ttttgccgac aaatacgatg
    11821 gggcttgccg acaggatgcg tgtcggaagt gtcccgtatt gcatactccg gataagcagc
    11881 gccgtcagca ggcagggaag gagcagggtc attcccggga taaacggatt gtgtttgtca
    11941 atcacgaaca ggcaggcaag caatgcgccg aagcagagtg atgaaagcaa ctgccgtttt
    12001 ccatttgctg tttgctgttt accgttttcc gtttgcccat aaaccgccag cagcgaacct
    12061 gccaacagct cgggaaacct cagtgtcgaa aggtaataag tattgggttg gttgaggatg
    12121 tcggtataaa acccgcttgg caaaaacgat gaggcagtca aaatcagaaa caggatgatg
    12181 ctgatgttac gcagcacccg tagcgatttg gtttttttgc agcagaatat cagcaaaagg
    12241 ggatacagga ggtaatactg ttcctctacc gccaaagacc aaatatgcag tacggggttc
    12301 tcgttggcac tcaaatcgaa atacccctgc tgaaacccca aataaatatt ggacaagaaa
    12361 accgcagaaa gctccacggt tttccgcatt tggttgaaat cttcgtaaag gaagatttga
    12421 gaggcaatca ccgaagccag cgacacggcc gcaataaagg caggataaat ccgcttaatc
    12481 ctgcgggtat aaaaatcccg gaaagaaaaa gaaccgtccc gtatttcggc aagaatgatg
    12541 ccggtaatga ggaatcccga gatgacaaag aaaatgtcca cccccaggaa tcctccgggc
    12601 agccaacggt tattcaggtg gaaaataatg acggatagca cggcgacagc ccgcaatccg
    12661 tcaatttccg gtctgtatcg gacagcttgc ataaatatcg cccctgtata tccggtattt
    12721 taaaaatgcc gtctgaacgc gcgttcagac ggcatcggtt tcagtaaacg ctcaaatccg
    12781 tttcgccaac gcttcagcct tgcccacata cagtgcgggg gtcagctcaa gcaattttgc
    12841 tttggcttcg gtagggattt ccaacgattc aatgaagact ttcagcactt cgggcgtaat
    12901 gccgcctttg ccgcgcgtca ggtctttcag cttctcgtaa ggattggcga caccgtaacg
    12961 gcgcatcacg gtttgaatcg gctcggcaag cagctcccaa gtggcatcca aatcggcggc
    13021 cagcgcggcg gggttgggtt cgagcttgtt cagaccgcgc aaatgggcgg cgaaacccaa
    13081 cacggcatag cccacgccta cgcccatatt gcgcaatacg gtgctgtcgg tcaaatcgcg
    13141 ctgccagcgt gagacgggca gtttttccgc caaaaaaccc aatacggcgt ttgccatacc
    13201 gaggttgcct tcagagtttt caaaatcgat ggggttgact ttgtgcggca tcgtggaaga
    13261 accgacttcg cccgctttga ctttttgttt gaagtaaccc aaagaaatat aaccccaaac
    13321 atcacggttg aagtcgatga gaatcgtgtt gatgcggctg agtgtttgga aaaactccgc
    13381 catatagtcg tgcggttcga tttggatggt ataggggttg aaggtcagac cgaggctgat
    13441 ttcgacaaag ttgcggcagt gtgtttccca atccacatcg ggataggccg ccatatgggc
    13501 gttgtagttg ccgaccgcgc cgttgatttt gccgaggaac tcttgcgcct gaaggatttt
    13561 aaactggcgt tgcaggcggt acacgatatt ggcggtttct ttgcccaaag tggtcggcgt
    13621 ggcgggctgc ccgtgggtgc ggctcatcat cgggacggcg gcaaggtcgt gcgccatatc
    13681 ggtgagtttt ccgatgattt cggcaagttt gggcaggatg acggtttcgc gcgcttcctg
    13741 cagcatcagg gcgtgggaca ggttgttgat gtcttcgctg gtgcaggcga agtggatgaa
    13801 ctcgctggca gcagcgactt cgggcacttc ggcaaaacgt tttttcaacc aatattcgat
    13861 ggctttgaca tcgtgattgg tggtcgcttc gatggctttg acggcggccg cgtcttcaag
    13921 tgagaagttt tcaatcaccg tgtcgatttc ggcaagcgtt tcggcactga agggcggcac
    13981 ttcgacaatc ttcggctcgg cggcgagggc tttgagccag tttaattcga ctttgacgcg
    14041 cgccttcatc agaccgtact ctgaaaaaat cgggcgcaat gcttcaacgg attgggcata
    14101 acggccgtct aggggggaaa gcgaggcgat ggggttgatc atttcggcat cctgttcgga
    14161 aaagacatca aaaattgtta aattgtttgc aattatacat cactctcagg acgctatgcc
    14221 gtctgaagcc cgtgttccga cagatacgca acgatttcgt cttcatcgtc cgatatggcg
    14281 aacaaagaag cggccccttc ggaaatcagg ccgcgcgcca aaagctgcgc gtttatccac
    14341 tccgccaagc ccgaccaaaa cgcctttccg accaaaacga ccggacgcgg cggcactttg
    14401 cccgtctgca ccaaggtcag gatttcaaac aattcgtcca gcgtcccgaa gccgcccggc
    14461 atcacgatat atgcttggga atagcggaaa aacaccgcct tgcgttcggc aaaacgggaa
    14521 aaccgcaagg cgatgtcctg atacggattc ggtttctgct cgtgcggcaa aacgatgttc
    14581 agccccaccg aaaccgactt cccggcaaac gcgcccttgt tcgccgcctc cataatcccc
    14641 ggcccgccgc ccgaaataac ggcaataccc gaatccgaca gccgcctcgc cagacggcag
    14701 gcaaacgtat aatccgcatg attctgcggc gtgcgcgcgc tgccgaagat actgactgcc
    14761 ggaaacacgc ccgccaatac ttcgtctgcc tgccggcgtt cggcatcgta acgcgcctgc
    14821 tccggcacgc ggtttacatt ctccattccg tcctccgttt aaaaacggcg attgtacccc
    14881 gtcaaaaac
//
LOCUS       DAWFAN010000036        14877 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWFAN010000036 DAWFAN010000000
VERSION     DAWFAN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14877
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.036"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(1..682)
                     /locus_tag="WGO65_000778"
                     /pseudo
     CDS             complement(1..682)
                     /locus_tag="WGO65_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
     gene            complement(768..1190)
                     /locus_tag="WGO65_000779"
     CDS             complement(768..1190)
                     /locus_tag="WGO65_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327098.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITPGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            1502..2812
                     /locus_tag="WGO65_000780"
     CDS             1502..2812
                     /locus_tag="WGO65_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ0327099.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDGLKKLVAKVLALPEYRDFTIEADAQELFVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            2926..5931
                     /locus_tag="WGO65_000781"
     CDS             2926..5931
                     /locus_tag="WGO65_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0327100.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     SKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDGLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLSFLLQHGL
                     TYVKASQGLVQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKHGIVPKFYFIVDRLDLLKQAQREFTARDLIVHTIDSREAFAADIKSAQTLHN
                     HSGKPEITVVNIQKFKDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     INAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSIDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDDSLGAMVVC
                     DSSEQARQLFEHFQTASNHNLTAVLILHDIGTKEERDQWVKDFKAGKIDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIQSEFDKTNRAY
                     WDELSNELGDEIGNYSQLFKTTEEIEQEIAGIKDALFEFDRENAEVFRHQIEQIADKK
                     QLLALKKALQTARELYNVLRLQGSHEFLECLDFDKLNLLYREAVSRLDTLNIIEKQQQ
                     GNIAHLLNEALEDIYFQFVKISEAELKLADDLKDIMRKVREGLAGNIDQDDPEYISLR
                     EELERIFKKKNLAEVGQEEMQANIATLDTVYAQIKELNRKNDLLRHKYGGDTKYARIH
                     KRLLENPALYGDKLKVFNALNGVKTDADQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQAINRLLVREYLKESGRI"
     gene            6061..7296
                     /locus_tag="WGO65_000782"
     CDS             6061..7296
                     /locus_tag="WGO65_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0327101.1"
                     /translation="MIEKRLIDISKNISSGITPLRSNDEFWTDGTIPWLKTEQLGEKY
                     IFDTNEYITEKALQEANVKIFPENTLSIAMYGEGKTRGNVSILKRPMATNQACCNIEL
                     DEEKVSSEYVYYFLKTQYENLRGLSSGIRKNLNTNDIKNFVVRLPENLKTQQSIAAVL
                     SALDKKIALNKQINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGEMVFDETLKRE
                     IPKGWEVKSLWKIAKYFNGLALQKYRPENELDDFLPVIKIREMNEGVSSNTERAKTNI
                     PKEAIIDDGDILFSWSATLEIKIWSQGKGALNQHIFKVTSSEYPKYFFYFELLNYLKH
                     FKMIADLRKTTMGHITQDHLKQAYICIPSQPLLEKLEKIVTPIFQKILITQKQNHQLT
                     QLRDFLLPMLMNGQVSVAE"
     gene            7345..8946
                     /locus_tag="WGO65_000783"
     CDS             7345..8946
                     /locus_tag="WGO65_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0327102.1"
                     /translation="MTEQHFTEQTKALIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKENPDEEIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEV
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPYHKDASGRLKKFDFIVSNPPFKLDFSDFRDQLESDENRERFFAGIPKIKAKD
                     KDKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAQSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKANKDNVVLIDASGLGEKIKDGKNQKTVLSREEEQKICN
                     TFTHKQAVEDFSVVVGYDEIKAKNYSLSAGQYFEVKIDYVDISAEEFEQKMAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            complement(9042..10379)
                     /gene="ribB"
                     /locus_tag="WGO65_000784"
     CDS             complement(9042..10379)
                     /gene="ribB"
                     /locus_tag="WGO65_000784"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ0327103.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(10593..11607)
                     /locus_tag="WGO65_000785"
                     /pseudo
     CDS             complement(10593..11607)
                     /locus_tag="WGO65_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(11763..12356)
                     /gene="ribA"
                     /locus_tag="WGO65_000786"
     CDS             complement(11763..12356)
                     /gene="ribA"
                     /locus_tag="WGO65_000786"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ0327104.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(12349..13026)
                     /locus_tag="WGO65_000787"
     CDS             complement(12349..13026)
                     /locus_tag="WGO65_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327105.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWRSDEDWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            13180..13264
                     /locus_tag="WGO65_000788"
     tRNA            13180..13264
                     /locus_tag="WGO65_000788"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13214..13216,aa:Leu,seq:tag)
     gene            13784..14818
                     /gene="purM"
                     /locus_tag="WGO65_000789"
     CDS             13784..14818
                     /gene="purM"
                     /locus_tag="WGO65_000789"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ0327106.1"
                     /translation="MSISLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         4030 a         3582 c         3543 g         3722 t
ORIGIN      
        1 cgcaaatgcc gttttgtaca attccaaaag ttttcaattc cgttaatgcg attttgccgt
       61 ttggcgaaat gcgtactgtt ccagtcgtgg attgaacccc caccctgtat agttctttcg
      121 aagcattggg gtattgtttt ttcaaagcat cgattaaagc cgtattggtg tccggatttt
      181 taacctggac aatattgtcc ccttcggctt caagcgttgc ttttgcagaa tgcgacgcga
      241 taatgccttt tacggaggcg tggtttttta catttaaatc aacttcatta tttcccttga
      301 cggctttgag cttgatagtg ctgatgccat caagggaagg ttgggtaagg tcgcttgatt
      361 gattattgat gaaaatgccc gtcgtgcgtc cggaataggg ggagttggac tggagaccat
      421 ttacgcccaa tcccaagccc ttgccctcag tggcgatgga tattttattg ttattccccg
      481 ttaatacggt attggattta aaagccgttt caatgcccct taaaaaactt gtactctcaa
      541 catttcctgt cggcataggg tcggcgagtt cccctttaat ctcaatcaca ttatcgctgt
      601 cttcggcagt cagatgaaca tcggtattgt tgaggttgtg aataccgaaa tggtgtgtgt
      661 ctttatgaac gttaacgttc atattgaaca aattgcctgt ttttgaatga aaggatatgc
      721 tgcctttgtc attttcatcg gggggctgat agtgttgaca taccgcacta aggcactatt
      781 gtttgtcggt atttttttcc actggctaca tcttgtaccc catcaacatc gggaacattc
      841 aaattaaatt gaatttttcc tttggcatcc aaatggatat ttccggaatg gggcttcacg
      901 tcaacagatt ggagattatt tttaaataga atatcattat tgctttttac caataattca
      961 ggcgtatatc ccaattgaga gtctgcaaaa gattggatgc cgtaaattcc atttaccgtt
     1021 aaatttccat tatcattttt accaggggta ataagttcta attttctaaa gttttcacct
     1081 tttttccacc aaggcacaat atagtttttt acagtgattt tttcatcttt aagggttata
     1141 agactatcat cgtctctttg cggatcagaa aataaagaca cccattccat ctcttgaata
     1201 ttagtatatt catgggcttc cgcctcaaaa cataatgccg attgaatcaa caaagcaatg
     1261 gcagataaag caaattttct attttagtct ccttaatgtt taattgaaac gctgatttgg
     1321 gtattgtcat gccgatgcgg aatcggtctc cgtgccggat atattgtgtg aagtctctcg
     1381 tcttttccag tatgcgccta atatcggctt aaggcaaacg gaggggcgga atccgtatgc
     1441 ggcacgccgc cgttccctgc cgtataatca tattgtttga aaacctgttg acggagccgt
     1501 tatgaccgat ttgtttgccc gagaacccga cgcgccgctt gccgaacgat tgcgtccgca
     1561 tacgcttgac gacgtggtgg ggcaggaaca cctcatcggc gaaggtaaac ctttgcgcgt
     1621 ggcggtagaa ggcggcaagc cgcattctat gttgctgtgg gggccgccgg gcgtgggcaa
     1681 gacgacgttg gcgcggattt tggcgcagag tttcaatgcc cagtttttgc ctgtttccgc
     1741 cgtgttttcc ggcgtgaagg acatccgcga ggcaatcgat aaagccgaaa tcgctttgca
     1801 gcagggacgc gcgacgattt tgtttgtcga tgaagtccac cgcttcaaca aggcgcagca
     1861 ggacgcgttt ttgccgcatg tcgaaagcgg tttgctaacc tttattggtg cgacgacgga
     1921 aaatccgtcg tttgaagtca atcccgcgct gttgagccgc gcgcaggtgt atgttttgca
     1981 gcccttgtct tcagacggcc tcaaaaagct ggttgccaaa gttttggctt tgcccgaata
     2041 ccgggatttc acgattgaag ccgacgcgca ggagctgttc gtcaataccg ccgacggtga
     2101 tgcgcgcaga ttgttgaatt tgttggaaca acttttacgc gccgccgata cacgtcgtct
     2161 gaaaaactta accgccgaat ttctcgccga cagtctcggg gcgcaaatcc gccgtttcga
     2221 caaaggcggc gagagcttct acaaccaaat ctccgccctg cacaaatccg tgcgcggttc
     2281 gcatccgaac gccgcgctgt attggttctg ccgtatgctc gacggcggca ccgacccgcg
     2341 ctacctcgcc cgccgcatcg tgcgtatcgc ttgggaagac atcgggcttg ccgacccgcg
     2401 cgccttccaa atcgccaacg atgccgccgc caccttcgaa cgcttaggct cgcccgaagg
     2461 cgaactggct ttggctcaag ccgtgctgta ccttgctgcc gccgccaaat ccaacgcggg
     2521 ctacaaggca tacaaccaaa tgcgccactt cgtcaaagaa aacgccagcg acgaagtgcc
     2581 cgtccacctg cgcaacgccc cgaccaagct gatgaaggaa ttgggctacg gacgcgaata
     2641 ccgctacgcc cacgacgaac cgaacgccta cgccgccggc gaaagctata tgcccgacgg
     2701 tttggacgaa ccggacttct accaacccgt cccgcgcggg ttggaaatca aaatcggcga
     2761 aaagctggaa tggttgaaat cgctggacga agaagtatta aaggcaaaat gaagcaatgc
     2821 tgtctgaagc agaacagtat ggcaaagccg gtacaccgaa aaggcgtatc gggtttttca
     2881 ggtgttataa tccgtttcaa taaattttga tgaatcatta cgtttatgtc ccaaactcac
     2941 aacgaaaatt cccgcgtcaa aatccccgcc gttttgcacc tgatgcgctt gggctacgac
     3001 tacctctcgc tcaaaaacga aaattgggat aaaccaacca acatctttcc caaaatcttt
     3061 atagaaagcc tctgccgcat caatcccgat ttgtcgccgg acgatgcacg ccgtttgctg
     3121 accgacatac gcttagaact ggacaacgaa gacttggggc aaaagtttta cgaacgcctt
     3181 accaatcaat ccggcggcaa gaaactgatc gacttccaaa attttgataa caacagcttc
     3241 cacgttgtaa ccgaattgcc ctgtatcaac ggcgatgaag aattccgccc cgacattacc
     3301 ctgctggtaa acggcatgcc tttggttttt atcgaagtca aaaagcccaa caacaaaggc
     3361 ggaatcggcg aagagcggga gcgcatgggc aaacgtgcga aaaattccaa atttcgccgt
     3421 ttcatcaaca tcacccaatt catgattttt tccaacaaca tggaatacga cgacggcgca
     3481 accgaaccgg cacaaggcgc gttttacgct tcgtctgctt acggtaagcc cgtgtttaac
     3541 tacttccgcg aagaacataa attaaatctt gccgaattat tgaacacgct ttcagacggc
     3601 ctcgaaaaca acgtcctgca agacaacaac ctgcccgtta tcaaacacag ccccgaattt
     3661 atcagcaata aatcgcccga tacgccgacc aaccgcatcc tgacttcgct gctttgccgc
     3721 gaacgtcttt cttttcttct gcaacacggg ctgacttacg tcaaagcaag tcaaggcttg
     3781 gtgcaaaaac acatcatgcg gtatccgcag ctatttgcca ctctcgccat tgaaaaacat
     3841 ttggcaaacg gcggcaaaaa aggcgtgatt tggcataccc aaggctcggg taaaaccgcg
     3901 cttgcctatt acaatacccg ctacctgaca cactattacg ccaaacatgg catcgtgccg
     3961 aaattctatt tcatcgtgga caggctcgat ttattgaaac aggcgcagag ggaatttacc
     4021 gcccgagact taatcgtcca taccatcgat agccgcgaag ccttcgctgc cgacattaaa
     4081 tccgcccaaa ccctgcacaa ccactcaggc aaaccggaaa tcaccgtcgt caacatccaa
     4141 aaattcaaag acgatcccga tgtcgtcgct cgcaacgact acgaccttgc catccagcgc
     4201 gtctattttc ttgatgaagt acaccgcagc tacaacccca aaggctcgtt tcttgccaac
     4261 ctcaaccagt cagacataaa cgccgtcaaa attgggctga ccggcacgcc gcttatcggt
     4321 gtaaccgcag gcaacgtcaa cactcgcgag cttttcggcg actacatcca caaatactat
     4381 tacaacgcct ccatcgccga tggctacacc ctgcgcctga ttcgcgaaga aatcggcagc
     4441 cgatacaaag cccaattaca agaagcactg gcgcaacttg aaatcgaaaa gggcagcatt
     4501 gaccgcaaag aaatctacgc ccatccgcac ttcgtccacc cgatgcttga ctacatcttg
     4561 gacgactttg ccaaattcag aaaaaccaac caagacgaca gcctcggcgc catggtcgtt
     4621 tgcgacagct cagagcaggc acgccagctt tttgagcatt ttcagacggc ctcaaaccac
     4681 aatctgaccg ccgtgctgat attgcacgat atcggcacca aagaagaacg cgaccaatgg
     4741 gttaaagact tcaaagcagg caaaatcgac atcctgtttg tgtacaacat gcttttgacc
     4801 ggctttgacg cgccgcgcct gaaaaagctc tacttaggca gactcatcaa agcccacaat
     4861 cttttgcaaa ccctgacccg cgtcaaccgc acctacaaat cataccgtta cggctatgtt
     4921 gtcgattttg ccgacatcca aagcgaattt gacaaaacca accgcgccta ttgggatgaa
     4981 ctgtccaacg aattgggcga cgaaatcggc aattacagcc agcttttcaa aaccaccgaa
     5041 gaaatcgaac aagaaattgc aggcattaaa gatgccctgt ttgaatttga tagagaaaac
     5101 gccgaagtat ttcgtcatca aatcgaacaa atagcagata aaaaacaact tcttgccctg
     5161 aaaaaagccc tgcaaaccgc cagagagctg tacaacgtcc tgcgcctgca aggcagccat
     5221 gagtttctcg agtgtctgga ttttgacaaa ctcaatcttt tatatcgtga agctgtttca
     5281 cgcttagata ccttaaacat tattgaaaaa cagcaacaag gcaatatcgc ccatttgctc
     5341 aatgaagcat tggaagacat ttacttccaa ttcgtcaaaa tcagcgaagc cgagctcaag
     5401 cttgccgacg atttgaaaga catcatgcgc aaagtgcgcg aagggctggc aggcaatatt
     5461 gaccaagacg accccgaata catcagcctg cgcgaagagc tggaacgcat cttcaagaaa
     5521 aagaacctcg ccgaagtcgg gcaggaagaa atgcaggcaa acatcgctac gctcgatact
     5581 gtctatgccc aaatcaaaga gctgaaccgc aaaaacgatc tcttgcggca caaatacggc
     5641 ggcgacacca aatacgcccg catccacaaa cgcttgctgg aaaatcccgc cctctacggc
     5701 gacaaactta aagtcttcaa cgcgctaaac ggcgtcaaaa ccgatgccga ccaaaaagtg
     5761 ctggatatgg agcagatttt ggataaccaa aattactttg aaaaacaaat gcagggcatc
     5821 gtattaaaac gctttaggac ggaacagcaa tttcccgttc agcccgccga catccaagcc
     5881 atcaaccgcc tgttggtgcg ggaatattta aaagaaagcg ggcggatttg aggaagcggt
     5941 ttttcaggca gcctttacgg gcgtattgca tacgttcgga atgggcaata aaagtgcggt
     6001 cagatttggg aatgtttttt cagacggcct ttaaagagtt tcaaaagatg aagttttaag
     6061 atgatagaaa aacggttaat tgatatttca aaaaatattt caagtggcat aacaccattg
     6121 cgaagtaatg atgaattttg gactgacgga acaatccctt ggttaaaaac agagcaactt
     6181 ggtgaaaagt atatttttga tacaaatgaa tatattacgg aaaaagcttt gcaagaagca
     6241 aatgtgaaaa tttttcctga aaatacacta tctattgcaa tgtatggaga aggcaaaact
     6301 cgaggtaacg tttcgatatt gaagcgaccg atggctacta atcaagcctg ctgcaatatc
     6361 gaactggatg aggagaaagt aagctcagaa tatgtgtatt attttttaaa gacacagtat
     6421 gaaaatttaa gagggttgtc ttcaggtatc agaaaaaacc taaatacaaa cgatattaaa
     6481 aattttgttg tcaggctgcc tgaaaacctc aaaacccaac aatccatcgc cgccgtctta
     6541 tccgctttgg acaaaaaaat cgccctgaac aaacaaatca acgctcgctt ggaagagatg
     6601 gcgaaaaccc tgtacgacta ctggtttgtg cagtttgatt tccccgacgc caacggcaaa
     6661 ccctacaaat cctcaggcgg cgaaatggta tttgacgaaa ccttgaaacg ggaaattccg
     6721 aaggggtggg aggtgaagag tttatggaaa atagcaaagt attttaacgg attagctttg
     6781 caaaaatatc gtcctgaaaa tgaactcgat gattttttgc cagttattaa aatcagagaa
     6841 atgaatgaag gtgtttcttc aaacactgaa agagctaaaa caaatatccc aaaagaagca
     6901 attattgatg atggggatat tttattttct tggtctgcga ctttagaaat caaaatttgg
     6961 tctcaaggta aaggggcttt aaatcaacat atttttaaag taacttcttc cgaatatcca
     7021 aaatactttt tttattttga attattaaac tatttgaagc attttaaaat gattgctgat
     7081 ttaagaaaaa cgacgatggg acatattacc caagaccatt tgaagcaagc gtatatctgt
     7141 attccttctc aaccattgtt agaaaaattg gaaaaaattg ttactcctat ttttcaaaaa
     7201 atattaataa ctcaaaaaca aaaccaccaa ctaacccaac tacgcgattt cctactgcct
     7261 atgctgatga acgggcaggt atctgtggcg gaataagcca gattcgagaa tcggacctac
     7321 aaaataaaac aaacgagata aaaaatgact gaacaacact ttaccgaaca aaccaaagcc
     7381 ctgattgaca gcttaaaaac catttgcgcc aactatggct taggcaatga cggcaacgag
     7441 ttcaaaatca tcagtcaggc atttttgtat aaattcttaa acgataaata cgattttgaa
     7501 gttaaaaaaa ttcgcaaaga aaatcccgac gaggaaattg agttcatcaa tatggatatt
     7561 gagggaaaaa cagccgtttt gaagcccgag cattccataa aatatctgtc cgagcggcaa
     7621 aacggtgcag attttgccaa actctttgac gatacgctga ccgacatcgc cgcgcataat
     7681 gcggaattgt tttcagtgaa aaccgaaggc ggggcgaaaa tcgtgttgtt cgagcgtatc
     7741 agtcaatata ttactgatga aggtcgccgc gacgattttt gccgcgcgct gatttccaag
     7801 ctggcgggtt ttagctttga ggtgattttt gcgcaaaaat ttgatttttt cgccacgatt
     7861 tttgaatacc tgattaaaga ctacaacagc aattcaggcg gcaaatacgc cgaatactac
     7921 acgccgcacg cagtagcgcg gattatggcg gatattttgg tgccggagga agtgcgcggg
     7981 caaattcgca gcgtggacgt ttacgacccg tcggccggtt cgggaacgct gttgatgaac
     8041 gttgcccatg ccatcggtga agataaatgt atgatttata cgcaggatat ttcgcagaaa
     8101 tcgtccaatc ttttacggct gaatctgatt ttgaacaatt tggtgcattc actgaataat
     8161 gtggttcagg gcaatacgat tttgtcgccg tatcacaaag acgcttcagg ccgtctgaaa
     8221 aaatttgatt tcattgtttc taatccgccg tttaaattgg atttcagcga tttccgcgat
     8281 cagctggaaa gcgatgaaaa ccgcgaacgc ttttttgcag gcattccgaa aatcaaggcg
     8341 aaggataagg ataaaatgga aatttatcag ctttttatcc agcatatctt gtttagcctg
     8401 aaagaaaatg gtaaagcggc gattgtattg ccgaccggct ttatcactgc ccaatcgggc
     8461 atcgataaaa aaatccgcga atatttggta gaaaacaaaa tgcttgccgg cgtggtttcc
     8521 atgccttcca atatttttgc caccacggga accaacgttt ctattctgtt tatcgataaa
     8581 gccaataaag acaacgtagt attaatcgac gcatcgggct tgggtgaaaa aatcaaagac
     8641 ggcaaaaacc aaaaaaccgt actttcccgc gaagaagaac aaaaaatctg caataccttc
     8701 acgcacaaac aggcagtgga agatttcagc gtggtggtcg gctatgatga gatcaaagcc
     8761 aagaattaca gcttgtcggc aggtcaatat tttgaagtaa aaattgatta tgtggatatt
     8821 tccgccgagg aatttgagca gaaaatggcg ggattttcgg cagatttgga caagctcttt
     8881 gcggaatctg ccgaattgga aaaagagatt aaggatagat tagcaatgtt gaaattcaat
     8941 tcataaatac tgacaaatta aactggccaa acttaaaagg ccgtctgaaa attttcagac
     9001 ggccttttgt ttcatttgtt ttgagaatca atcaagcaag attatttttc agcttcttca
     9061 aagccgacga cttcgaggcc gaagccggtg aggccggtga aggaggaagg ctggccgagg
     9121 acgcgcagtt ttttgacgtt gaggccggcg aggatttgtg cgcctatgcc gtagcttttg
     9181 ctgtcccatt tgtaggcttg gtttgcgcct ttaggcaggg tgcggtcgag cagggatgcg
     9241 ccgtcttcgg tgcggtgcag aaggatgacg acgccgcttt ctgcctgttg gacgcgctcg
     9301 agtgctttgg gcaatgacca tgaatggcgc gggttggctt gaatgaagtc catcacactg
     9361 aagggttcgt ggacgcggac gagggtttcg gtgtcggcgg agggcgtgcc tttgacgagg
     9421 gcgaggtggg tttcgccgga gagtttgtcg acgtaaacgt gttgttggaa ctcgccccac
     9481 ggggtttgta caggcgcatt gcccatgtct tcaagcaggc tttcggtacg gctgcggtat
     9541 tcgatgaggt cggcaatcgt gccgattttg agcttgtgtt cttcggcgaa tttcatcagt
     9601 tcgggcatac gcgccatcgt gccgtcgtcg ttgatgattt cgcaaataac ggaggcagga
     9661 atcagcccgt tcatttgcgc caggtcgacg ccggcttcgg tgtgtccggc gcggacgagt
     9721 acgccgcctt tttgggcgcg aagcggaaag atatgaccgg gttggacgat gtcttcgggt
     9781 ttagcggtcg gggaaacggc ggtttgaata gtcagggcgc ggtcggcggc ggaaatgccg
     9841 gtggtaatgc cgtgtgcggc ttcgatggag acggtaaagt tggtgccgta ttgcgcgccg
     9901 tttttctgag tcatcatggg caggccgagt ttttcgacca tttcgccgtc catcggcagg
     9961 cagaccaagc cgcgcgcgtg tttaatcatg aagttgatgg cttcgggcgt gacgaattgc
    10021 gccgccatca gcaggtcgcc ttcgttttct cggtcttcgg catcggtgat gatgaccatt
    10081 ttgccggctt tgatgtcggc taggatttcg gggatggggg aaatatggga catggtgtgt
    10141 tttcctgtgt gttcgggcgg gcgggcggtg tgttcggtat cgagccagaa ggcgggcatt
    10201 tttgcagcct gttcgatttt acgggctttt ttctcgccga aggatttgtt tttcaaaagg
    10261 gcggagagtt cgcctgtgtt gagggcaacg gcttcggcaa aacgggtttg caaaccgccg
    10321 tagtgttctg ctatccattg ccgcaggttg cggcggcgca gaccggctgt gtcggtcatc
    10381 gtttcatcct ttgtagaaat cggatgtagc ggattgtaag cgcgtattta ccaaaaagca
    10441 aatctgtttt ttcgctaaac cgaattattt gctttttggt aaacagatgc cgtctgaagc
    10501 cggattcgtg gcttcagacg gcattgccgc aaccccgtcg gcggggcggc tgtttcttcc
    10561 cgcccgcttc cgattcggga tttgccggaa gcttattccg aaaaaaattt ttgataaacg
    10621 gccttgattg ccgggtacag cataggcggt acgagcaggc gcaatatcgg caatcctatg
    10681 atgaatgcgg tcttcccgat aaaaaccgga cggattgcct tcgcgccgaa tatctttctg
    10741 catttttctt taaatgaaaa atatttccgg tagatgacga taatgttttt ggaaaaataa
    10801 agccttacta atatcgacct cagatagccg atattgtcga agacaaattt ttgcaaaccg
    10861 ccgtagatgg gggaattttt gttttcgact aaaaaatcca caccccggtc gagatgcgtc
    10921 agcacatcgc tgaaattttt gtatttgatg ttgtggctga tagagccgcc gagaacgcgg
    10981 tactggtaaa aagggttatc gtaaaaggca aaagtcttga taaaacgcgc caattgcaaa
    11041 ttgtacggga aatcctcgtg aatgtatcct tttgggaaaa acagattgtt tttgatgatt
    11101 atttcccgcc tgacgatttt tgtccacgcg ttggcgatat agtaccgccc ctccaccagc
    11161 gtttcaaaat ggcggacaaa atcattatcc gaaaagtccg cccctttggg gatgacgtgg
    11221 taattgaggg acgagggatg caggatcaaa tcaacctttt tgtctgcaag ttgttgtaaa
    11281 tcaaagagaa tccccccccc ccgcgttttt tgaacggttg gtatcggccc aatagtcgtc
    11341 gctgtccaaa aagattaggt aatcgccttt tgccgcccgg ataccggcgt tgcgggcatc
    11401 cgacagcccg ccgttttctt gatgaatcac ttttatatgc ggatatttgc ctgcatattc
    11461 gtcgcaaatc ttcccgcagc cgtccggcga accgtcatcg accaaaatca tttcataatc
    11521 ggtaaaattt tcggcaagca cggaatccac gcagcagcgg aggtattttt ccacattgta
    11581 aatagggacg atgatggaga aaatcataaa tatcaatcca ttatattaag atgtttgcgc
    11641 gtatgcctca aacccgcgct cgcaatgcgt ttgcatccac tataaaactt attgctttat
    11701 caaggtatgg aaacctgttt cccgaaaggc ggcgcaggat gcccgttccc tgcaactttg
    11761 ccttattccg acatcagatg tcccagcttg tctgctttgg tttggaggta gcgtttgttt
    11821 tcaaggtttt ccccgacgtg cagggggatg cgttcgacca cgttaatccc cgcatctttc
    11881 agggtttgga ttttttcggg gttgttggtc agcagtttga ccgagcggat gcccagatat
    11941 tcgtagatag attgcgccaa acggaaatcg cgggcatcga cgggcagccc gagtgccaaa
    12001 ttggcttcaa ccgtatccat accttggtct tgcagatgat aggcgcggat tttgttaatc
    12061 agcccgatgc cgcgtccttc ctgacgcaga tagacgatga tgccgcgccc ctctgtctgt
    12121 accgccctca tggccgcttc aagttgaggt ccgcagtcgc attttctcga gaacagcgca
    12181 tcgcccgtca ggcattcgga gtggatgcgc gtcagaaccg gattgccgtc tgaaaaatta
    12241 ccgacggtca gcgcgacgtg ttcctgcccg tttgcctctt caaagccgtg catcgtaaat
    12301 acgccccatt cggtcggcag gcggcaggat gccacatggt tcaacatttc agacatcttc
    12361 actcccatct tttccggcag acatacccaa cagcagtttc aaaggttcgg acaaagccaa
    12421 ggcaagagcc acccattcca cctgtgctgc cgtacccgca cactctgcgc attcaaattc
    12481 cacatgaacc acgcccaaca caccgccact ttccgtgcag accggaatgg aaatttgcgc
    12541 cgccgaagca tgattgcgtt ctcccgaaag ctcccccaaa tccaaccaac ggcgtacatc
    12601 cgaggcaaca ttcatccaac cgctttgcgc cgaacggcaa gccagcgaaa ccttgcctgc
    12661 cgcttcatcc aaatcccaca ggttttccaa aacctcgccc tgtcgggaca ggcatatcag
    12721 ttggaaagcc tggttttcag atggcatcaa ggcatagacc gccgcactcc gcacgcctgt
    12781 cgagcgcgaa aacaccgaat ccaaagccat gaaaagccgt ttcagtgcat cttcgcggac
    12841 attgtcgcac gacaggtaat cggcaagttt ccaatcctca tcactgcgcc acaaaaccga
    12901 acggtctatc gaagccgtcc ccatatccat taccgtctgc gccgtcagat acgccgcccg
    12961 aacctcgtca agcggcagct tcaaaccctg agtcagcaga aaatccttaa tcaaagcagc
    13021 aggcataccc aaattccgtc aataaaaaca aaaaaccgcc ggattccgat gtcaggcgga
    13081 cgaaaaacag attttaccgc cccaaacccc aagccatcaa cagccccgcc gaaaaccttg
    13141 cacaaaaaaa caaaatctgt ataatcgtcc aattcccatg cggacgtggc gaaattggta
    13201 gacgcaccag atttaggttc tggcgccgaa aggtgtgaga gttcgagtct ctccgtccgc
    13261 accaataatg ataaataaat catatagtta atgcttatcg ggacaaatta gtgccacaaa
    13321 acataagtcg tctgcttttc agcgggcggc ttttttatct gcctatatgt ttcaacacgc
    13381 agaacggtac ataaagtgcc gccatttgtt ctaccccgaa tcagaagcgg gtgcttccgc
    13441 ccgcaatcag attcaccgcc cctaatgccg gggtttcaac tggaaagaag aaaatattga
    13501 ttttgatgat ggcatcaaaa aaccccttcc gcccgtctgc caaaaggaat ggttaagaaa
    13561 agcaatgcgt cttttgatgg aacgcgatat ataaggaatt gaggggaagg gcaggcgggt
    13621 ttctgcccgt taatcctgcg gacgatcccg ccaaacccga cacgcgcggg catatggcgg
    13681 attgtttaca atattgtcta aaaatctgaa cttgcaaacg cgaaataagg tagaatacgc
    13741 cccgttattt taccgtccaa tccccattta ccaaggaaaa atgatgagta tttcattgag
    13801 ttaccgcgat gcaggtgtcg atatcgacgc aggcgaccaa ctggtcgaaa acatcaaacc
    13861 ttttgccaaa cgcacgatgc gcccggaagt attgggggat ttgggcggct tcggcgcatt
    13921 ggtcgaaatc ggcaagaaat ataaaaatcc cgtattggtc agcggtacgg acggcgtggg
    13981 taccaagctc aaacttgcct ttgattggga taaacacgat acggtgggca tcgaccttgt
    14041 tgcaatgagt gtcaacgata ttttggttca aggagcagaa cctctgtttt tcttggatta
    14101 tttcgcctgc ggcaaattgg atgttccgcg cgcaaccgat gtcatcaaag gcattgcaca
    14161 aggttgcgaa gaatccggct gcgccctgat tggcggggaa actgccgaaa tgccgggcat
    14221 gtatcccgtc ggagaatacg atttggcggg ttttgccgtc ggcgtagtgg aaaaagagaa
    14281 tgtcattacc ggccgcagca tcggcgcggg cgatgtggta ttgggtttgg cttccaacgg
    14341 cgcacattca aacggctatt cccttatccg taaaatcatc gaacgcgaca atcccgattt
    14401 ggatgccgag tttgataatg gcaaaacctt gcgtgaggct gttattgcgc cgacccgtct
    14461 gtatgtgaaa ccgattcttg ccgctttgga aaaatttacc attaaaggta tggcacacat
    14521 taccggcggc ggcattaccg aaaacgtgcc gcgcgtgttg cctgaaaaca cggttgcaca
    14581 aatcgatgct aaatcgtggg aattgcccaa gctcttccaa tggcttcaaa aggcgggcaa
    14641 tgtggaaacc caagaaatgt accgaacctt taactgcggc atcggcatgg tcgttattgt
    14701 tgctgccgaa gatgccgatg cggttcaggg tctcttgggt gaacaaggtg aaacagttta
    14761 ccgtttaggt ttgatccgtg agcgtcaggg agacgagcat caaacccagg ttgcctgatt
    14821 gcttctatag cgaaatgccg tctcaaacat gggtttggac ggcattttta tagagga
//
LOCUS       DAWFAN010000037        14063 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWFAN010000037 DAWFAN010000000
VERSION     DAWFAN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14063
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.037"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(69..2045)
                     /locus_tag="WGO65_000790"
     CDS             complement(69..2045)
                     /locus_tag="WGO65_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ0327107.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKVLVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIVRVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPIYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGETGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WGO65_000791"
     CDS             2194..3033
                     /locus_tag="WGO65_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ0327108.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARTRYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3232..4263
                     /gene="gap"
                     /locus_tag="WGO65_000792"
     CDS             3232..4263
                     /gene="gap"
                     /locus_tag="WGO65_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0327109.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FASKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGKLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4415..5140)
                     /locus_tag="WGO65_000793"
     CDS             complement(4415..5140)
                     /locus_tag="WGO65_000793"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ0327110.1"
                     /translation="MRIPLLAPDNYAFPDPTYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(5210..5644)
                     /gene="fur"
                     /locus_tag="WGO65_000794"
     CDS             complement(5210..5644)
                     /gene="fur"
                     /locus_tag="WGO65_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ0327111.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHSEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5863..6240
                     /locus_tag="WGO65_000795"
     CDS             5863..6240
                     /locus_tag="WGO65_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0327112.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            6250..7059
                     /gene="dapB"
                     /locus_tag="WGO65_000796"
     CDS             6250..7059
                     /gene="dapB"
                     /locus_tag="WGO65_000796"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ0327113.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7056..7301)
                     /locus_tag="WGO65_000797"
     CDS             complement(7056..7301)
                     /locus_tag="WGO65_000797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327114.1"
                     /translation="MPRKQLYRISFSVETPPLGHLSFGAVESDFIWEGRNPFQIRTTH
                     RATLYVSSRVLKHMPIRSLSAKCRLNNIFSDGILYGG"
     gene            complement(7319..7729)
                     /locus_tag="WGO65_000798"
     CDS             complement(7319..7729)
                     /locus_tag="WGO65_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327115.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPMPNYSEWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGGRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            complement(7731..7976)
                     /locus_tag="WGO65_000799"
     CDS             complement(7731..7976)
                     /locus_tag="WGO65_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327116.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAELARVGLRL
                     KYNGLLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(7984..9156)
                     /gene="lpxB"
                     /locus_tag="WGO65_000800"
     CDS             complement(7984..9156)
                     /gene="lpxB"
                     /locus_tag="WGO65_000800"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ0327117.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLKRYPAARFLLPAATEATKRRLAEILQRSEFAGLPLTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9204..10196)
                     /locus_tag="WGO65_000801"
     CDS             complement(9204..10196)
                     /locus_tag="WGO65_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0327118.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            10690..13449
                     /locus_tag="WGO65_000802"
     CDS             10690..13449
                     /locus_tag="WGO65_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ0327119.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSENATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         3542 a         4552 c         3291 g         2678 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccggttt caccgaatcc cgcttcgtcg tcgctgccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 atgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgttttg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 acaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca ataccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aagatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacgggacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaacac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcac ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgcattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcgtc
     2341 cgcgaccttg ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcac gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acgcccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat acctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaagcc
     3061 gcttcagacg gcattttatc aagctctccg tccgccgcac ccgtgccgtc cgcatcttac
     3121 cgcccaccct tccggccgcg ccgttttcaa taaaatatta attacacgcc actacaaatt
     3181 tgctataatc cgccccgaaa atctacccaa ccctcaacaa aggaacgaac catgggcatc
     3241 aaagtcgcca tcaacggcta cggacgcatc ggccgccagg ttttgcgcgc catctacgac
     3301 tatcagattc aagaccaact ccaaatcgtc gccgtcaacg ccagcggcag ccttgaaacc
     3361 aacgcccatc tcaccaaatt cgacaccgtg cacggacgct ttgaagccga cgtatcccac
     3421 gacggcggca acctcatcgt caacggcgac aaaatcccct tcttctcgac ccgcaaccct
     3481 gccgaactgc cgtggaaaga actcggtgtc gatttggtca tggaatgcac cggcgcgttc
     3541 gccagcaaag aaaaagccaa agttcatctg gaaagcggcg cgaaaaaagt cctcatttcc
     3601 gcaccgggcg gcgacgatgt cgatgcaacc gtcgtgtacg gcgtgaacga cagcatcctg
     3661 accgccgaca tgaccgtcgt ttccaacgct tcctgcacca ccaactgcct ctcgccggtt
     3721 gccaaagtgt tgagcgaaag cgtcggcatc gtcaaaggcg cgatgaccac catccacgcg
     3781 ctgaccaacg accaaaccgt taccgacgtg cgccacaaag acctgcgccg cgcccgcagc
     3841 ggcgtggaaa acatgattcc gaccaaaacc ggcgcggcaa aagccgtcgg tttggtactg
     3901 cccgaattga aaggcaaact cgacgggctt gccatccgcg tgccgaccgt caacgtatca
     3961 ttggtagatt taagcttcca agccgcgcgc gacacgacag tcgaagaaat caacgcactg
     4021 atgaaagccg cctcggaagc aggcgcgctc aaaggcgttt taggctacaa caccctgccc
     4081 ttggtttcca tggacttcaa ccacaccacc gaagccagcc acttcgacgc aacactgacc
     4141 aaagtcgttg acggcaacat gattaaagtc ttcgcatggt atgacaacga atggggcttc
     4201 agctgccaaa tgctgaacac cgcacgccgt atgttcggac ttgaagtgcg cccgctcaaa
     4261 taagcaataa accgtcaaac aaaatgccgt ctgaaacccg atgttttcaa gttccagacg
     4321 gcatttttca ttttttggca gtgattttgc ccggctgtcg ccatttccaa ttttaatcca
     4381 ccatatttga acctgaccgg agcataagcc gccatcaaag ccccttgcac gccacttcgc
     4441 gccgcccgat ttcctctttc aacggcacgg cgttcaacat ccgcagacgt tcggcaaaat
     4501 ccgcaaacgg cagcagctcc gaaccgaaac ggcgcatatg ttccgtgtcc tgctggcagt
     4561 ctatcagttc cacacccaaa tccgccaaaa acggcacggc gcaggcaaac gcgattttcg
     4621 acgcatccgg ttgtaatgcg aacatcgatt cgccgtaaaa caccctgccg atttgcacgc
     4681 cgtaaaagcc gcccgccaac ctcgtttcac cgctttcatc gggataatgg cactcgaacg
     4741 aatgcgcgtg tcccatttcg tgcagcttca aatatgccgt ctgaaactcg ggcgcaatcc
     4801 aagttccgtc ctgattcggg cgcgccgctg ccgcacaatg cgcgaccact tccgcaaaac
     4861 agccgttgac cgcaacccga tagctgccgt tgcgcagcgt tttcgccagc gagcgcggaa
     4921 cgtgcagacg ttcgggaaca atcaccgcac gcgggccgac cgcataccaa aaaaaccaac
     4981 cgtcccgaga aaaccacgga aacacgccgt tccgatacgc ctcgagcagc cgccccgcat
     5041 ccaaatcgcc gctcacgccg accagcccgt cgcaccgggc caaagcatag gtaggatcgg
     5101 gaaaggcata attgtcaggg gcaagcagcg gaatacgcat ggcagttctc caaaaacaca
     5161 gacggcatcc aaaatcatga atgccgtctg aacaacaaac cgtccggatt taacgtttgc
     5221 ccttggcctg acagtcgctg cacacgccgt acatataaag cgcgtgatcg acgatgcggt
     5281 agccgttttc ctcagcgatt ttgtcttgca gggcttcgat ttcgggattg tggaattccg
     5341 ttacctcgcc gcacttcacg cagacgatgt ggtcatggtg gtcgcctttg tccaactcat
     5401 aaaccgcctt gcccgtttca aaatgatggc gttgcagaat acccgcctgc tcaaactggg
     5461 tcagcacacg gtaaatcgtc gccacaccga tttccacacc ctcttccaac aaaatgcggt
     5521 acacatcttc cgcactcaaa tgctcttccg aatgcgtctc gaacaaatcc aaaatcttca
     5581 aacgcgggcc ggtaaccttc agaccgctgt ctttcagttg tgcaatattg ttgaattttt
     5641 ccataatatt caatacccct gtaaaacaat agacgttata atacgcaatt tcggcctgtt
     5701 tgcccactat cgcaccatag cagtttgcaa tagtaaaacc gcaccggcgg ccgatgcgcg
     5761 gacagggcgg ataccgctta atcgtatgat tatcgttcgc ttttataaaa tattcaagca
     5821 gtgttacact acccatcccg atttgcacag aaaggcattt ccgtgaacaa aaccctcatc
     5881 ctcgcccttt ccgccctcct cggccttgcc gcgtgcagtg ccgaacgcgt ttcactgttc
     5941 ccctcgtaca aactcaaaat catacagggc aacgaactcg aaccgcgcgc cgttgccgcc
     6001 ctccgccccg gcatgaccaa agaccaagtc ctgctcctgc tcggcagccc catactgcgc
     6061 gacgcattcc ataccgaccg ctgggactat accttcaaca cctcccgcaa cggcatcatc
     6121 aaagaacgca gcaatctgac cgtctatttt gaaaacggcg tactcgtccg caccgaaggc
     6181 gacgtcctgc aaaacgctgc cgaagccctc aaagaccgcc aaaacacaga caaaccataa
     6241 ggaacacaca tgacaccgct caaaatcgcc atcgccggcg caaacggccg tatgggacgc
     6301 gtattggttg aagccgtcaa caaccatccc gacaccgtcc tttccggcgc gctcgaacac
     6361 tcgggctcag aagccctcgg gctggacgca ggctacgccg tcggactcaa aaccggcatc
     6421 gccatttcag acgacgttga cgccgttctc gcacaaagcg acgtactcat cgacttcacc
     6481 cgccccgagc ccaccctcaa acacctgcaa aaatgcgttg aaaaacaagt caacatcatc
     6541 atcggcacaa caggcttcga cgatgcgggc aaagccgcta tccacactgc cgccgaacaa
     6601 acaggcatcg ttttcgccgc caacttcagc gtcggcgtca acctcacctt ccacatcctc
     6661 gacaccgtcg cacgcgtatt aaacgaaggc tacgacatcg aaatcattga aggccaccac
     6721 cgccacaaag tcgatgcccc cagcggcacc gcgttacgca tgggcgaagt catcgccggc
     6781 gcgctcggca gagacctcaa acaatgcgcc gtttacggcc gcgaaggcca caccggtccg
     6841 cgcgatccgt cgaccatcgg ctttgccacc gtccgcgcag gcgacatcgt cggcgaccac
     6901 accgccctct tcgccaccga cggcgagcgc gtggaaatca cccacaaagc cggcagccgc
     6961 atgacctttg ccgccggcgc cgtccgcgcc gcagtttggg taaacggcaa aacgggtttg
     7021 tacgatatgc aggacgtact cgggctgaac aaccgttaac ccccatacaa aatgccgtct
     7081 gaaaagatat tgttcagacg gcattttgcc gacaggctcc gtatcggcat atgtttcaac
     7141 acacgggacg atacataaag cgtcgcccta tgtgtcgtcc tgatttggaa gggattacgc
     7201 ccctcccaaa taaagtctga ttctaccgcc ccgaaggaca gatgtccgag tggcggggtt
     7261 tcaaccgaaa aggaaatacg ataaagttgc ttgcgcggca tgacaagtta cggctcgttt
     7321 aattaaaact ctcctgatct aaaaattcta actctatttc ccggcaaact atatctatac
     7381 taaaaccggt cggaagatca tacctaccgc ctatcacttc cagcgcacct ccataatcca
     7441 agatttcatc atcctgcttt aattctccaa gttccaatac atttttgata tttttatctt
     7501 ggttcttcgt ggcgtatcca tagataaatt tcagatagct gaactgcttg aatttaattt
     7561 ttacccattc tgaataattg ggcataggga tctcttgagg aaagtccgga taatccacat
     7621 tctggacgat gatatggaat tcatcgtttt tcaaagtata gtttaataga gtgccctcat
     7681 atatatacag aaattgataa tctttttttg gaatggtata agttgtcata ttattttacc
     7741 ttttcaaaaa caatgagttt aagatttttt gagtccaagt tggttctact actcttatga
     7801 aatttcagtt gtttaaactc ttcctttaac agaccattat attttaacct taatcccact
     7861 cgagccagtt ctgcttcatt tggaattcct cttaaatact tattgtttga tgtgccatta
     7921 atcacaattt ctcccccttt ttttgtaata cgtgctgctt cgggaaaata atccattaac
     7981 cgctcaacat cccgcctctt ccaaaaccgc gcgcgcggcc aaatccgccg tatctttttt
     8041 caacagcagg tgcaacgccc tgaaatcctg ttgcagcgcg gcaaccttat cggggtgttc
     8101 gtaccagtcc gccaacgccg ccgccagttt ttccggtacg gcatcatgtt gaagcagctc
     8161 cggcacagcc tccttaccca acaggatatt cggcaggcca acatgcggca ctttgatttt
     8221 gcgtttcaca taagcataag tcagcggcga aatcttgtag ctgatgacca tcggacgctt
     8281 gcacagcgcc acttccaaag tcgccgtacc gctcgttacc agcaccgcat ccgccgccct
     8341 gcacaccgtt tcagactgtc tgtcggttac cgtcagcggt aatccggcaa actccgaacg
     8401 ctgcaaaatt tccgccaaac gccgcttcgt cgcctccgtt gcggcaggca gcaggaagcg
     8461 tgcggcggga tagcgtttca acaacaataa tgccgtctga aaaaacaccg gcgccatata
     8521 gtcgatttcg ctgacgcggc tgccgggcag cagggcgaat acggggatgc cggcatccac
     8581 gcccaaagtt tgccgcgccg tttcacggtc gtcttccaag ggcataagct gcgccatcgg
     8641 atgaccgaca aactccgcac gtccgcccgc atcgagataa agctgcggct ccatcgggaa
     8701 caggcacagc acgcggttga cctgatgcac gattttgccc acgcgttccc gccgccacgc
     8761 ccacaccgac gggctgacat aatgcacggt cggaatgccc gcccgtttca gcttttccgc
     8821 cacacccaaa ttgaaatcgg gcgcatcgat accgacaaag acatcaggtt tcaacgacag
     8881 caaatcccgt accagccccc tgcgtatccg taaaatttcc ggcagccgcc tgaccacttc
     8941 gacaaagccg cgcaccgcca gccgctcctg atcataaagg ctctcgaaac cttccgcctt
     9001 catcagttcc ccgccgatgc cggtaaaccg cgcctgcggg caacgcttgc ggatggcgcg
     9061 tatcaggtgc gcccccaaca ggtcgcccga cgcttcgccg acactgacgg caatcaaagg
     9121 gcttttttta tcaaccatat tcgtctgtcc ccacatactt tcacgtcccg atgccgtctg
     9181 aaggcttcag acggcatcgg ggttcacatt ttcgtcccgt tttccaacat caccgcaaac
     9241 tgcgccaagt ccggcggcag ctccgccttc aacaccagcg gctcacccgt gagcggatgg
     9301 ttcaagtgca gctcggacgc gtgcaaaaac atccgcttca aacccaactt ctgcaaacga
     9361 cggttcgcct gataatcgcc gtagcgttcg tcgcccgcaa tcggacagcc ttgagattgc
     9421 aggtggacgc ggatttggtg cgtgcgcccc gttttcaacg tcgcccgcac caaagtcagg
     9481 tgcgacagcc cgacaccgtg caaaatgccg tctgaaaaac ggcttaacac acggaacacc
     9541 gtatgcgccg actgcccgtc cgcactgacg cgcaccatct tttcgccttg tgcgccggta
     9601 tatttgaaca ggggcagttt gacatggaaa ttgtcgtccg gcagtttgcc cacccccagc
     9661 gcaaggtaga tttttttggg gtggtcgtta cggatggctt cgtgaagttt gacgagcgcg
     9721 ctgcgtttct tcgccaccat caacaagccg ctcgtatcct tgtccaaacg atgaaccaac
     9781 tccaaatact tcgcctccgg acgggcgcgg cgcaactgtt cgataacgcc gaaactcacg
     9841 ccgctgccgc cgtggacggc aacgccggac ggtttgtcga tcaccaaaag cgcatcgtct
     9901 tcgtaaacaa cgtcaaacgc acgcgccggt acggcggcac gcctttcaga cggcatttcc
     9961 ttctccgcca cgcgcacagg cggaatccgc accgtatccc cctccgcaat acggctgtcg
    10021 ggtttgcagc gttttttgtt cagccgcacc tcgccggcgc ggataatgcg gtggatatgg
    10081 ctcttgggaa cccccttgag gatttttatc agatagttat caaggcgttg acccgcctca
    10141 tgttcggcaa cccctatcaa gctgaccgaa tctttgctta tttcgtgcgt tttcatctat
    10201 aatccgaaca tccgtttcag caaaaagcgc gcccgccgcg cctcctgaaa cggtttactt
    10261 taaaacggaa ttttatatta aaaacgcact ccgcaaagca ttttccctgc gcctgttccc
    10321 agccggcagg cgcagaacgt aatcaagttt gaattgattt tgccgtttcg gcgcggaagt
    10381 aagacggcag ccgggcccca agtcccaaac gcaaaccgcc cgaaaacaca ggcatcacga
    10441 taacaagaag aagccggcac ccatttttcc gaccataccg ctcggaacgc ggcagacaga
    10501 ccgccgcatc gatctttatt gccttcttta tccgaccctt ccgcacacgg ttcctcggaa
    10561 cgtgcatccc tgcggatttt caactcaaaa actgattacc ggttttatcc gaagacatcg
    10621 gaaaacccag ccttacggaa tgccgaaccg gaccggcgcg cccgaatgcc gcccgtccac
    10681 gaggtgatca tgaaaagaat gttattcaac gcaacgcagg ccgaagagct gcgcgttgcc
    10741 attgtcgatg gccaaaacct tttggacttg gacatcgaaa cgctgggcaa agaacagcgt
    10801 aaaggcaata tctacaaagg tatcattacc cgcatcgagc cgtcgctgga agcgtgtttc
    10861 gtcgattacg gaaccgaccg ccacggcttt ttgccgttta aagaagtctc ccgctcatac
    10921 ttccaagact acgaaggcgg acgcgcgcgc atccaagacg tgctcaaaga aggcatggaa
    10981 gtcatcgtcc aagtcgaaaa agacgagcgc ggcaacaaag gcgcggcgct gaccaccttc
    11041 atcagcctcg ccggacgcta tctggtattg atgccgaaca acccgcgcgg cggcggcgta
    11101 tcccgccgta tcgaaggcga agaacgtcaa gaactcaaag ccgctatggc gcaactcgac
    11161 attccgaacg gcatgagcat catcgcccgt accgccggca tcggccgcag cgcggaagag
    11221 ttggaatggg atttgaacta tctcaaacaa ctctggcagg cgattgaaga agcaggaaaa
    11281 gcgcatcacg acccttacct gctctttatg gaaagctcgc tgctcatccg cgccatccgc
    11341 gactatttcc gccccgacat cggcgaaatt ctggtggaca atcaagaagt ttacgaccaa
    11401 gttgccgagt tcatgagcta cgtcatgccg ggcaatatag gccgtctgaa actctacgaa
    11461 gaccacacgc cgctgttttc ccgcttccaa atcgaacacc aaatcgaaag cgcgttttcg
    11521 cgcagcgtca gcctgccctc cggcggcgcg atcgtcatcg accataccga agccctcgtc
    11581 tccatcgacg tgaactccgc acgcgccact cgcggcgcag acattgaaga caccgcgttc
    11641 aaaaccaata tggaagccgc cgaagaagtc gcccgacaaa tgcgcctgcg cgacttgggc
    11701 ggcttggtcg tcatcgactt catcgacatg gaaaacccca aacaccagcg cgatgtggaa
    11761 aacgtcctgc gcgacgcgct caaaaaagac cgcgcccgcg tacagatggg caaactctcc
    11821 cgtttcggcc ttttggaatt gagccgccaa cgtttgaaac cggctttggg cgaaagcagc
    11881 cacgtcgcct gtccgcgctg cgccggcacc ggcgtgattc ggggcatcga atccaccgcc
    11941 ctgcacgttt tacgcatcat tcaagaagaa gcgatgaagg acaacaccgg agaagtgcgc
    12001 gcccaagtgc ccgtcgatgt cgccaccttc ctgctgaacg aaaaacgcgc cgagctgttt
    12061 gcgatggaag agcgtttgga tgtcaacgtc gtcctgattc cgaacatcca cctcgaaaat
    12121 ccgcactacg aaatcaaccg catccgcacc gacgacgtag aagaagacgg cgaaccgagc
    12181 tacaaacgcg tcgccgagcc tgaagaagac gaatccgcca aaccgttcgg cggcgaaaaa
    12241 gccaaagccg cccgtcccga acccgccgtc aaaggcgtgc gccacaccag ccccgccccg
    12301 actgccgccc ccgagaaaaa aacctcttgg tgggacagct tcaaagcttg gctcaaacgc
    12361 attttcggcg gcagcgaaac ccaagccgcg cccgctgccg aaacctccga aaaacgcagc
    12421 acggcaaacc gcagcggcag ccgcgccaac aaccgccgcc aaaacccgcg ccgcagcaaa
    12481 cgcgaaggca gcaaagtaga agtccgcgaa gtggcaggca aaactgccgg acaggaagcg
    12541 cgtgccgaca aagccgaaac gcgcaacaac ggcaaccgcc gccgcaacga acgcggcgac
    12601 cgtgccgccg gacgcgccga cgaagcggaa atccaagacc gcaacataca gcctgccgca
    12661 accgttgcag atgccgcacc gtccgaaacc gaagtgcaaa ccggaaaacg ccgccgcaac
    12721 ggcagccgca gcgaacgcgg ccaaaccgcg ccggaaaccg ccaccgttgc cgaaacaacc
    12781 gttcagacag cggaaaacac gccgtccgaa ccgcataccg cagaagacaa aggcagcaag
    12841 cccaaatccg aacgcaaccg ccgcgaacgc gacagccgag atgccaaaga acgccgcgag
    12901 cgcaacaatc agcgcgaccg ccgtcaaaac ggcaaaaaac gcaatattcc gtctgccgcc
    12961 aaaatcgagc agtacctgaa tattcacgac accgccgaca aagtccgttc cgccgccgcg
    13021 cacgttttcg gcgaaaccga cgcaaacgcg ccgattaccg tcagcattgc cgatccggtt
    13081 gcagaaaggg atcttccgac agcatctccc gccgtttcaa acggcgacgc accggtttat
    13141 gatgcggcgg aaaaaatccg ccgtgccacc gccgccatcc tgcccgaagg cgcgacaccg
    13201 aaagccgaag cacaggaaat gccgtctgaa aacgcaacct ttacggctgc ggcggaacag
    13261 gcacgggaaa ccgcacaaac cggcggactc gtcctgatcg aaaccgaccc tgccgcattg
    13321 aaggcatggg cggcacaacc cgaagtccaa accggacgcg gtttgcgccg ttccgaacag
    13381 cccaagccgt ctgaagtcgc aaccgtccct gccgaagaaa tgatccaagt cgaaacccgg
    13441 caaggctgaa ccgacggcgg caaaaagagg ttctgttccg cagaacctct tttttacata
    13501 gattcggata cctgcaatgc cgtctgaaac ttcgccattc ccgtcattcc cgcaaccttt
    13561 cgtcattccc atgattcccg caacattccg tcattcccgt gaaaacggga atctagaacc
    13621 tcaaactttc ggataatctt tgaatattgc cgtcgcccaa aggcctggat tcccgcctgc
    13681 gcgggaatga cggtttagaa gttgcccgaa atctcaaaaa aaaccgaaac cgaacaagcc
    13741 agattcccgc aacattccgt cattcccgtg aaaacgggaa tctagaacct caaactttca
    13801 gataaccttt gaatattgcc gctgtccaat ggtctggatt cccgcctgcg cgggaatgac
    13861 ggtttagaag ttgcccgaaa cctcaaaaaa accgaaaccg aacaagccgg attcccgcaa
    13921 cattccgtca ttcccgtgaa aacgggaatc tagaacctct aaactttcag ataatctttg
    13981 aatattgccg ctgcccgaag gtctggattc ccacaacctt tcgtcattcc cgtgaaaacg
    14041 ggaatctaga acctcaaact ttc
//
LOCUS       DAWFAN010000038        13975 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWFAN010000038 DAWFAN010000000
VERSION     DAWFAN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.038"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(105..1430)
                     /locus_tag="WGO65_000803"
     CDS             complement(105..1430)
                     /locus_tag="WGO65_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ0327120.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1645..2484
                     /locus_tag="WGO65_000804"
     CDS             1645..2484
                     /locus_tag="WGO65_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ0327121.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            2481..3647
                     /locus_tag="WGO65_000805"
     CDS             2481..3647
                     /locus_tag="WGO65_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ0327122.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3911..5176
                     /gene="efeB"
                     /locus_tag="WGO65_000806"
     CDS             3911..5176
                     /gene="efeB"
                     /locus_tag="WGO65_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ0327123.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPVHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5245..5577)
                     /locus_tag="WGO65_000807"
     CDS             complement(5245..5577)
                     /locus_tag="WGO65_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ0327124.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNKTESAAAEAAEEV
                     RDANGAVLQNGDTIILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIACKIDGSAMNL
                     KSEFVKKA"
     gene            complement(5652..7022)
                     /gene="glmU"
                     /locus_tag="WGO65_000808"
     CDS             complement(5652..7022)
                     /gene="glmU"
                     /locus_tag="WGO65_000808"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ0327125.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVIGHGKEQVLNTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDASAA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKRQLAELERIFQTGQAQELLKAGVTLHDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVEIGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNRIGPYARLRPQ
                     ARLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNVEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(7077..7349)
                     /locus_tag="WGO65_000809"
     CDS             complement(7077..7349)
                     /locus_tag="WGO65_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ0327126.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPDQTDGSEQDVRGQNWQKPKFQNR"
     gene            complement(7342..8055)
                     /locus_tag="WGO65_000810"
     CDS             complement(7342..8055)
                     /locus_tag="WGO65_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ0327127.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDEVLTRLHGMPKTQNTLSR
                     LKGRKAVFSNGPSFYVRAIVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPENTKECYA"
     gene            8114..9115
                     /locus_tag="WGO65_000811"
     CDS             8114..9115
                     /locus_tag="WGO65_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0327128.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGMGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9209..10057
                     /locus_tag="WGO65_000812"
     CDS             9209..10057
                     /locus_tag="WGO65_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0327129.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10083..10568
                     /locus_tag="WGO65_000813"
     CDS             10083..10568
                     /locus_tag="WGO65_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ0327130.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLASVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10670..12238)
                     /gene="msrAB"
                     /locus_tag="WGO65_000814"
     CDS             complement(10670..12238)
                     /gene="msrAB"
                     /locus_tag="WGO65_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ0327131.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRIEVRSHAADSHLGHVFPDGPQDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12383..13648
                     /gene="ftsY"
                     /locus_tag="WGO65_000815"
     CDS             12383..13648
                     /gene="ftsY"
                     /locus_tag="WGO65_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ0327132.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         3011 a         3577 c         4178 g         3209 t
ORIGIN      
        1 tccgtcaaat aagaaaggaa ttttgtgcct gcggtatcgc aaaacttcgc cttaatgcgc
       61 ccgattgact agggatgggc ttcagatggc attgttttgc cggtttacgg gcggtattcg
      121 ggcttcatac cgttgggtag gagctgccag acgtaacccg tggttttctg tttgccggca
      181 agttcgccgg cttcgtcgcc gtatccccaa aaataatcca cgcgcaccgc gcctttaatc
      241 gcgctgccgg tatcctgcgc cataatcagg cggttgaggg ctttgcgggt aaccggatgg
      301 gcggtggcga caaataaggg cgcgcccaag gtaatgtagt gccggtcgac tgcgccggca
      361 tattccccca tcaacggcgt gcccagtgcg ccgacgggac cgtcattgct gcttccggca
      421 agctcgcgga aaaagatata gctggggttt tgacccaaaa cttcggcgag gcgttgcgga
      481 ttttgccgca tataggcttt gatgccctgc atggaggtct gcccgagctt gaggtagcct
      541 ttgtccgcca tatagcgtcc gatggaaacg tagggatgtt cgtttttgtc ggcatagccg
      601 atgcggatgt atttgccgga cggggttttc agacggcccg agccttggat gtgcataaaa
      661 aaaagttcga cagggtcttc ggcgtaaccg agtatcggtg ctttgccgtc aagcgcgccg
      721 ccgttgattt ggttgcgcgt gtggtagggg aggaagcggc ttccttcaaa cctgcctttg
      781 attgccgttg tgcgcgcagt gatggggaat tgggagaggt cggcggtatg tgtgccgccg
      841 gtattgtcga ttgtgccgct gttttttccc gtctgcctga tgcggacaag ggcttttccg
      901 ctccgcaaac cggcaggcag ggggacggag ataaaatcgt cgggaatacc gtaaatcggg
      961 aagcgggctt gtgccgtccg cctgtcgtcg cccttcagca ccggctcgta atagccggta
     1021 accgtaccgg caaggcttcc gttgcctgca acctgccacg gcgtgaaata gcgttcaaaa
     1081 aactgttttg cctgaaagga atggacgggg gtttgaaagg cttgggcgca cacatcctgc
     1141 cagccttggc ggtttttcaa attggcgcag ccgaggcgga aggattgcag gcttttggcg
     1201 aaatcctgcg ccgcccagtg gggcagggac aggtgcggca caacggtata aacggccccg
     1261 ccgccgccga ccgtcgttcc ggcggggtcg gggatgccga ccggccggtc cgggccgttg
     1321 atgacggatg tgtcgggttg cggaaaggtt tggatgctct tgctttggca ggcggcgagg
     1381 atggcggcgg cgatgccgca cagggcggcg cggaacaggt attttttcat aatggacaat
     1441 gttgccggca gtaataaaaa aaaaagatgg tttcgggcgg cgttgcggca gccgtggaga
     1501 ggggatttta acacagggcg cagctgcagc ctgcggaact ttccgccgcg cggcaatgca
     1561 gataaaaata acttgcattt gtatttacaa gcaatgaaaa tattccgata atatattatt
     1621 catcatcctt gttcgttcgc gtttatgctg gtcgcttttt taattatgtt gcgcgagggt
     1681 atcgaagccg cgctcatcgt cggcatcgtt gccggttttc tgaaacagtc cggacattcc
     1741 aaactgatgc ctaaggtctg gttcggggtc gtccttgctt ctttgatgtg tttgggcatc
     1801 ggatacggca tccattcggc aacgggcgag attccccaga agcagcagga gttcgtcgtc
     1861 ggcattatcg gtttggttgc tgtcgcgatg ctgacttata tgattttatg gatgaaaaag
     1921 gcggcgcgtt cgatgaagcg gcagcttcag gattctgtac aggcggcttt gaaccgtggc
     1981 agcggtcaag gatgggcctt ggtcggtatg gcgtttcttg ccgtggcgcg cgaaggtttg
     2041 gagagcgttt ttttcctgct tgccgtattc aaacagagcc cgacgtggca gatgccggcc
     2101 ggtgcggtag cgggggtttt ggctgccgcc gtgattggcg cgttgattta tcagggtggg
     2161 atgcgcctga atctggctaa gtttttccgt tggacggggg cgtttttgat tgtcgttgcc
     2221 gccggcctgc ttgccggctc gctgcgcgcg ctgcacgaag caggcatttg gaacgcgctt
     2281 caggacattg tgttcgactc atcaaaatat ttgcacgaag acagtccgtt aggtgtgctg
     2341 ctcggcggat ttttcggcta taccgaccat ccgacgcagg gcgaggcctt ggtttggctg
     2401 ctgtacctta ttcccgtcat gacttggttt ttgtgcggca gcaggccgtc tgaaacttta
     2461 acccgtaaag aggagctgaa atgagaaaat tcaatttgac cgcattgtcc gtgatgcttg
     2521 ccttaggttt gaccgcgtgc cagccgccgg aggcggagaa agctgcgccg gcagcgtccg
     2581 gtgaggcgca aaccgccaac gagggcggtt cggtcagtat cgccgtcaac gacaatgcct
     2641 gcgaaccgat ggaactgacc gtgccgagcg gacaggttgt gttcaatatt aaaaacaaca
     2701 gcggccgcaa gctcgaatgg gaaatcctga aaggcgtgat ggtggtggac gagcgcgaaa
     2761 acatcgcccc cggactttcc gataaaatga ccgtcaccct gttgccgggc gaatacgaaa
     2821 tgacttgcgg tcttttgacc aatccgcgcg gcaagctggt ggtaaccgac agcggcttta
     2881 aagacaccgc caacgaagcg gatttggaaa aactgtccca accgctcgcc gactataaag
     2941 cctacgttca aggcgaggtt aaagagctgg tggcgaaaac caaaaccttt accgaagccg
     3001 tcaaagcagg cgacattgaa aaggcgaaat ccctgtttgc cgacacccgc gtccattacg
     3061 aacgcatcga accgattgcc gagcttttca gcgaactcga ccccgtcatc gatgcgcgtg
     3121 aagacgactt caaagacggc gcgaaagatg ccggatttac cggctttcac cgtatcgaat
     3181 acgccctttg ggtggaaaaa gacgtgtccg gcgtgaagga aattgcagcg aaactgatga
     3241 ccgatgtcga agccctgcaa aaagaaatcg acgcattggc gtttcctccg ggcaaggtgg
     3301 tcggcggcgc atccgaactg attgaagaag tggcgggcag taaaatcagc ggcgaagaag
     3361 accggtacag ccacaccgat ttgagcgatt tccaagccaa tgtggacggt tcgaaaaaaa
     3421 tcgtcgattt gttccgtccg ctgatcgagg ctaaaaacaa agccttgttg gaaaaaaccg
     3481 ataccaactt caaacaggtc aacgaaattc tggcgaaata ccggactaaa gacggttttg
     3541 aaacctacga caagctgggc gaagccgacc gcaaagcgtt acaggcctct attaacgcgc
     3601 ttgccgaaga ccttgcccaa cttcgcggca tactcggctt gaaataagcc gcaagcgttc
     3661 agacggtatt tggcggcaga taccgtctga agttttatag tggattaaca aaaaccagta
     3721 cggcattgcc tcgccttgcc ttgccgtact atttatactg tctgcggctt cgtcgccttg
     3781 tcctgatttt tgttaatcca ctatatccgc catatattgc agggcaggat ttcaacctgc
     3841 cgctatcggt taatggaaaa acggcgtgca gggataccca tcctgctgca cggatattga
     3901 aggaaacacc atgagcaaaa accaacccgc acaaccgacc aggcgcactc tttttaaaac
     3961 cgcgatcgca gccggagcag tcggcgcaat cggaggttat ctcggcggca aaaaacaggg
     4021 cgaaaccgcc gaacgcaccg ccgaaagcca acactcgccc caagcctatc cctgctacgg
     4081 cgaacatcag gcaggcatcg ttacgccgca gcaggcgttt tcgattatgt gcgccttcga
     4141 cgtaaccgcg caaagtgcca agcagctgga aaacctgttc cgcacgctga ccgcccgcat
     4201 cgagtttctc acccaaggcg gcgaatacca agacggcgac gacaaacttc cacccgccgg
     4261 cagcggcatt ttgggcaaag ccttcaaccc cgacgggttg accgttaccg tgggggtggg
     4321 cagcagcctg tttgacggcc ggttcggact caaagacaaa aaaccggttc atttgcagga
     4381 aatgcgcgac ttctccaacg ataagctgca aaaaagctgg tgcgacggcg atttgagcct
     4441 gcaaatctgc gccttcaccc ccgaaacctg ccaagccgcc ctgcgcgaca tcatcaaaca
     4501 caccgtccaa accgccgtta tccgttggag tatcgacggg tggcagccta aatccgaacc
     4561 cggcgcgatg gcggcgcgca acctgttggg cttcagggac ggcacgggca accccaaagt
     4621 ttccgacccc aaaactgccg acgaggtttt gtggacgggt gtggccgcca acagcctcga
     4681 cgaaccggag tgggcgaaaa acggcagcta tcaggcagtc cgccttatcc gccactttgt
     4741 cgagttttgg gacaggacgc cgcttcaaga gcaaaccgac attttcgggc ggcgcaaata
     4801 cagcggtgcg ccgatggacg gcaaaaaaga agccgaccaa ccggattttg ccaaagaccc
     4861 cgaggggaat accacgccca aagacagcca tatacgcctg gcgaatccgc gcgatcccga
     4921 attcctcaaa aaacaccgcc tcttccgccg cgcctacagc tattcgcgcg gactcgcctc
     4981 aagcggacag cttgatgtcg ggctggtgtt cgtctgctat caggcaaacc ttgccgacgg
     5041 attcatcttc gtgcaaaacc tcctcaacgg cgaaccgctg gaagaataca tcagcccctt
     5101 cggcggcggc tatttcttcg tgctgcccgg cgtggaaaaa ggcggctttt tggggcaagg
     5161 gctgctgggc gtataaatcc gccatataaa aaacgccgtc tgaatccgat aaggggttca
     5221 gacggcattt tactgtttgg gcggtcaggc ttttttgacg aattcagatt ttaaattcat
     5281 cgcgctgccg tcgattttgc aggcgatgtt gtgatcgcct tcctgcaggc gtatgccttt
     5341 gactttcgta ccttgtttga tcgtcatcga gctgcctttt accttgaggt ctttgatgag
     5401 gatgatggtg tcgccgtttt gcagcaccgc gccgttggca tcgcgcactt cttcggcggc
     5461 ttcggctgcg gcggattcgg ttttattcca ttcgtgggcg cattcggggc agatgtattg
     5521 tccgccgtct tcgtaggtgt attcggaggc gcattgcggg caagggggta atgacatggt
     5581 ttgccgtcct tatcggatgt ttgttttggg gtgccgtctg aaacctgaaa ccggcttcag
     5641 acggcatagc tttattgttt gtctttttca ggacgcaccc agccttcgat gacggtttgg
     5701 cgggcgcggg cgagggcgag tttgttgtct tcgacattgc gggtaatcgt gctgcccgcg
     5761 cctgtggtta ctttgttgcc gagggtaacg ggggcgacta ggacgcagtt tgaaccgatg
     5821 cgcacttcgt cgccgatgac ggttttgtgt ttgtgcacgc cgtcgtagtt ggcaataatc
     5881 gtaccggcgc cgaagttggt tttgctgccg acttcggcat cgccgatgta ggtaaggtgg
     5941 ttggctttgg tgcctttgcc gatggcggcg tttttgattt cgacgaagtt gccgacgtgt
     6001 acgtcgtctg caaggcgggc ttgcggacgc aggcgggcgt acgggccgat tcggttgttt
     6061 tcgccgactt cgcagtcttc gaggtgggag aagggggcga ttttgctgtt tgcaccgatt
     6121 ttggcgtttt tgatgacgca gtttgcgccg atttcgacgt tgtcgccgat ttcgacttcg
     6181 ccttcaaaga tacagttcac atcaattacg acatcctgtc cgtgtttcag acggcctcgt
     6241 aaatcgaaac gtgccgggtc gtgcagggta acgcctgctt tgagcaattc ttgcgcctgt
     6301 ccggtttgga agatgcgttc gagttcggcg agctggcgtt tgttgttcac gccggcggcg
     6361 aggtgggagg cgcggacttg gacgggatga actttaatac cgtcggcaac ggctttggcg
     6421 atgaggtcgg tcaggtagta ttcgccttgg gcattgttgc tggaaaggct gttcagccag
     6481 ttttccagtt tggcgttggg caggacgaga atgcctgtgt tggtttcttt cacggctttt
     6541 tgggcggcac ttgcgtcttt ttcttcgaca atggcggtta cgctgccgct gccgtcgcgg
     6601 atgatgcgcc ccaaacctga cgggtcggcg ggcacgtcgg tcaacagccc gacttcgttg
     6661 cctgcggctt cgagcagggt ttcgagggtt tcaacgtcaa ttaaaggaac gtcgccgtac
     6721 aacaccagcg tgcggccttc ggcggcaagg tggggcaggg cggttttgac ggcgtggccg
     6781 gtgccgagct gttcggtttg ttcaacccaa acgacatcgc gtttgacagt attcaaaact
     6841 tgctctttgc cgtgtccgat gacgacgcag atgttttgcg gattcagtgc ggcggcggtg
     6901 tcgataacgc gctcaaccat gggcttgccg ccgatgcagt gcagcacttt gggcattttg
     6961 gaatacatgc gcgtgccttt gccggcggcg aggataacga tgtttaaagt gttttgcggc
     7021 atgacggttt cctgtgcaat gccgtctgaa agggcttcag acggcatagg gtgggtttat
     7081 cggttttgaa atttgggctt ttgccagttc tgtccgcgta cgtcttgttc ggaaccgtcg
     7141 gtttggtcgg gcagcacggc atggcgttcg gggcggtatt ggttgtagtt catatttttc
     7201 gagtagctgc cgtcttggta ataaacgggc gtgccggcgg gatatttttg acggacggcg
     7261 gtcttgccgt tgccgtcttg ataagtttcc cacgcgcagc ccgacaaaag agcggcggca
     7321 gtcatcagga taaggaaggt tttacgcata acattctttc gtattttcgg gggttgtgcg
     7381 agtattgtaa tgattttggc ggtgttctga caaagtttct gcataccgag ccagttgcgc
     7441 catatcgctt acggaagcat cgataaaggg caggtcgtgc acttttgcgc cgaaccggac
     7501 ggttttcatg cccaatgctt ttgcctgatg caggttgtcc gcgctgtcgt ccaccataat
     7561 gcagcattcg ggcggtacgt ccaaaaggcg gcagacattg agatacgctt gcggattggg
     7621 tttgtacagc agtccgaaat catctgtgcc gaacagtgcg tcgaaacggt tttccaaacc
     7681 gagtgcgccg acaatggcgc ggacgtaaaa cgacgggccg ttggaaaaaa ccgccttgcg
     7741 cccttttagg cggctcaggg tgttttgtgt tttcggcata ccgtgcagac gggtcaaaac
     7801 ctcgtcaatc ggatggcttt cgcgcaaaaa ttcggcgatg tcgatttcgg gatggtggat
     7861 ttgcagtccg gcgagcgttg cgccgtagcg gtgccaatag tcttgacgca ggtcggacgc
     7921 ggcggattcg gagagtttga ggcggcgtgc catatagcgt gtcatggcgc ggttgatgag
     7981 tgtgaagatg ccggcatcgg cattgtgcag cgtgttgtcg aggtcgaaca gccacacggt
     8041 cgggttttct tgcattttga accgtgaaaa tttgttagaa tgttatttta cagcgaatag
     8101 aggaggactc ggaatgaaac ggaaaatttg gctgctgccg ctgctggcgg tttcggcata
     8161 cctgcaggcg cagacggaag tcaggctggc ggtgcataag tcgttcagcc tgcccaaagg
     8221 gctgattgcg cgcttcgagc gggcaaacga tgcgaaggtg tcgattattc aggcgggcgg
     8281 cgcgaacgaa atgctcaaca aactgatttt gagccgcgcc aacccgattg ccgacgcggt
     8341 gtatggtttg gacaacgcca atatcggcaa ggcgcgggaa atgggcattt tggcggcggc
     8401 gcaacccgaa tccgcccccg tcgcggtcgg gctgccttcg gctttggcgg tcgattacgg
     8461 ctatgtgtcc atcaattacg acaaaaaatg gtttgaaggc aaaaagctgc ccctgccgca
     8521 aaccctgcag gatttgaccc gccccgaata taaaaaccta ttggtcgtgc cgtcccccgc
     8581 cacgtcgtcc ccggggctgg gcttcctgat ggcgaacatc agcgggatgg gcgaagaagg
     8641 cgcgttcaaa tggtgggcac agatgcggca gaacggcgtg aaggtcgcca aaggctggag
     8701 cgaggcgtat tacaccgact tttcgcacaa cggcggcgcg tatccgctgg tggtcggtta
     8761 tgccgccagt ccggcggcgg aagtgtattt ttccaaaggc aaatacagcg agccgccgac
     8821 gggcaacctg tttttaaaag gcggcgtatt ccgccaggtc gaaggcgcgg cagtcttgaa
     8881 gggcgcgaaa cagccgaagc tggcggcaaa actggtgcaa tggctgcaaa gtcgggaagt
     8941 gcagcaggcg gttccgtccg aaatgtgggt ttaccccgcc gtcaaaaaca cgcgcctgcc
     9001 cgacgtgttc cgcttcgccc aagccccgac gcacaccacc gcccccgcgc agcgcgatat
     9061 tgatgcgaac cagcgcggat gggtttcccg ttggattaga acggttttga aataaaacaa
     9121 acataccgcc ccgcagggct tcatacggca tttttacacc tgtgccgatt acgccgcacg
     9181 gggcggatgt tcgatcaaga ggaaaacaat ggacttcaaa caatttgatt ttttacacct
     9241 gatcagtgtt tccggttggg agcatctggc tgaaaaggcg tgggcgttcg ggctgaacct
     9301 tgccgccgcg ctgcttattt ttttggtcgg aaaatgggcg gcgaaacgca ttgtcgctgt
     9361 gatgagggcg gcgatgacgc gcgcgcaggt cgatgccacg ctgattagtt ttttgtgtaa
     9421 tgttgccaat atcggcttat tgattttggt gattattgcc gcattgggca gattgggcgt
     9481 ttccacaaca tccgtaaccg ccttaatcgg cggcgcgggt ttggcggtgg cgttgtcctt
     9541 gaaagaccag ctgtccaatt ttgccgccgg tgcgctgatt atcctgttcc gcccgttcaa
     9601 agtcggcgat tttatccgcg tcggcggttt tgaaggatat gtccgagaga ttaaaatggt
     9661 gcagacttct ttgcggacga ccgacaacga agaagtcgtg ctgcccaaca gcgtggtgat
     9721 gggcaacagc atcgtcaacc gttccaccct gccgctttgc cgcgcccaag tgatagtcgg
     9781 cgtcgattac aactgcgatt tgaaagtggc gaaagaggcg gtgttgaaag ccgccgtcga
     9841 acaccccttg agcgttcaaa acgaagagcg gcaggccgcc gcctacatca ccgccttggg
     9901 cgacaatgcc atcgaaatca cattatgggc ttgggcaaac gaagcagacc gctggacgct
     9961 gcaatgcgac ttgaacgaac aagtggtcga aaacctccgc aaagtcaata tcaacatccc
    10021 gttcccgcaa cgcgacatac acatcatcaa ttcttaaacg ccgtctgaaa ggggagtggg
    10081 aaatggacgc gctgcacacc atcgcccgaa acctgacgaa aaaacgtcaa accgtaagct
    10141 gtgccgaatc ctgcacgggt gggctgcttg cctccgtatt cacaagcgtt gcaggcagtt
    10201 cgcaatggtt cgatcaaagt tttgtaacat acagcaacaa agccaaagaa gaccgcttgg
    10261 gcgtgttgcc cgaaaccctg ctcgaacacg gcgcggtcag ccgccaaacc gtctatgaga
    10321 tggcgcgcgg cgcgaaagcc gtggcgcagg cggattacgc cgtcggtatt tccggcatcg
    10381 ccggtccggg cggcggcagc gaaagcaaac ccgtcggcac ggtttggttc gggtttgcct
    10441 ttccgggcgg aagttgcgaa gcaatgcgcc gttttgacgg caaccgcgaa tccgtccgcg
    10501 cgcaggcggt cgccttcgcg ttggaacggt tggcggggct gattgaaaac ggcggcgatg
    10561 ccgtctaaac aaaatctccg tctggacaaa atccccgccg gataaaaaat accgtctgaa
    10621 acgttgcagg tttcagacgg tattttgtcg ggttaggcgg cggtgcggct tatttcactt
    10681 tactcttcaa cgcgccgtag cctgccgcgt ccatttgttc cagcgggatg aatttcaaac
    10741 tcgcgccgtt gatgcagtag cgcagaccgc ctttgtcttg agggccgtcg gggaagacgt
    10801 gtcccaagtg cgaatcggcg gcgtggcttc tgacttcgat gcggcgcata ttgaagctga
    10861 aatcatcgtg ttcggtaacg gattttgcat caatcgggcg cgtgaagctc ggccagccgc
    10921 agccggaatc atatttgtcg gcggagctga acaaaggttc gccgctgaca acgtccacat
    10981 aaatgccggg tttgaacaaa tggtcgtatt cgtggctgaa ggcatattcg gtcgcgctgt
    11041 tttgggtaac ttggtattgc tcttcggtca gggtgcgttt gagttcggcg tcactcggtt
    11101 ttttatacgt tgccgcatcg aagcctttgc cttgcggggc ggctttggtt ttgcccggca
    11161 gcggttcgtc ggctttgcgg atatcgatgt ggcagtagcc gttggggttt ttaatcaggt
    11221 agtcctgatg gtattcctcg gcatcgtaga agtttttcag cggctcgttt tcaacaacga
    11281 ggggcagttg gtatttttgc tgctcgcgtt tgagggcggc ggcgatgacg gctttttcgg
    11341 cggggtcggt gtagtacacg ccgctgcggt attgcgtacc ggtgtcgttg ccctgtttgt
    11401 tgaggctggt cggatcaacg acgcggaaga aatattgcag gatgtcgtct aggctgagtt
    11461 tgtcggcatc gtaggtcact ttaacggttt cggcgtggcc cgtatggcgg taggacacgt
    11521 cttcatagct cggatttttc gtgttgccgt tggcgtagcc ggataccgcg tcaaccacgc
    11581 cgtcgatgcg ttggaaatag gcttccaagc cccagaagca gccgccggcg aggtaaatgg
    11641 tgcgcgtgtt catgattttt gaatcctttt tctgagtgtc gggtttgtag aacgaatgtt
    11701 tcaagctgcc caaatcggca ttcgggtcgc ggattaacgc caacgcctgc gcttcgttga
    11761 tgctgccttt gacgatgcgc tgcacgtcgc cgtctttacc gattaacgcc cacgaagggt
    11821 aaacgctgat attcaggctt tgggcgatcg tgccgccgtt gtcggttacg acgggcagct
    11881 tgggataatt caaaccggca taccattttt ggaagtcgcc gtcttttttc tcgtgcaaaa
    11941 agcccggaga cgcgacggta atcaggttgg cggagctgaa ttttgcatct tgcgcccatt
    12001 tttcggtctg tcccaattcg gacagacaca aaggacacca gctcgcccaa aatttaatca
    12061 gcgtcggttt gtcttttttc aagtaaacac tggcggggcg gttgtccgca gttttcaaag
    12121 tggataaagt gtgcggcacg gtcgcggctc cggcatcgac gattttgggc gaacaagcac
    12181 ccagcgcaag caggcagccg aacttggcgc aaagggaaaa gaaagtacgg tgtttcattt
    12241 tgagtttcct gtgtggacgg tttgcatgat tagacgtttg agatgccgaa accttacagc
    12301 tcggattttc agacaacctt gccgcgtaaa atacgctaca atacgccctg tttcaagttt
    12361 ctaaaattaa aaggaaaatt caatgttcag cttcttccgt cgcaagaaaa aacaggaaac
    12421 gccggctctc gaggaggctc aaattcagga aaccgcagca aaagcagagt ccgaaaccga
    12481 tcaagtaatt gaaaatatta aagaagatgc tgaatcttta gcagaaagcg tcaaagggca
    12541 ggtcgaatct gccgttgaaa ccgtcagcgg tgcggttgaa caggtaaagg aaaccgttgc
    12601 cgagatgctg tctgaagcag aggaagcggc ggaaaaagca gcggaacaag tcgaagcggc
    12661 aaaagaagcc attgccgaaa ccgtcggcga ggctgtcggg caagttcaag aagccgttgc
    12721 aacaactgaa gaacacaagc tcggttgggc ggcgcgtttg aaacaaggtt tgaccaaatc
    12781 gcgcgacaaa atggcgaaat cgctggcggg cgtgttcggc ggcggacaaa tcgacgaaga
    12841 tttgtacgaa gagctggaaa ccgtgctgat taccagcgac atgggcatgg aagccaccga
    12901 atacctcatg aaagacgtgc gcgaccgtgt cagcctcaaa gggctgaaag acggcaacga
    12961 attgcgcggc gcgttgaaag aagccttgta cgacctgatt aagcctctgg agaaaccttt
    13021 ggttttgccc gaaaccaaag agccgtttgt catcatgctt gccggcatca acggcgcggg
    13081 caaaaccacg tctatcggta aactcgccaa atatttccaa gcgcagggca aatctgtgct
    13141 gcttgccgcc ggcgacacct tccgcgccgc cgcccgtgag cagcttcaag cttggggcga
    13201 acgcaacaac gttaccgtga tttcacaaac cacgggcgat tccgccgccg tgtgcttcga
    13261 tgccgtccaa gccgccaaag cgcgcggcat cgacattgtg cttgccgaca ccgccggccg
    13321 cctgcccacg cagcttcatt tgatggaaga aatcaaaaaa gtgaaacgcg tgctgcaaaa
    13381 agccatgccc gacgcgccgc acgaaatcat cgtcgtgctt gatgccaaca tcggtcaaaa
    13441 cgccgtcaac caagtcaaag cctttgacga cgcattaggg ctgaccggtt taatcgttac
    13501 caaactcgac ggcacggcaa aaggcggcat cctcgccgcg cttgcctccg accgccccgt
    13561 ccccgtccgc tatatcggcg tgggcgaagg catagacgac ctgcgcccgt ttgacgcgcg
    13621 cgcgtttgtg gacgcactgc tggattgagc cgaaatgccg tccgaaaaca gcagaccgat
    13681 gccgtcattc ccgcgcaggc gggaatctag accttgggat aacagcaata ttcaaaggtt
    13741 ataaaagacc cgtcattccc gcgcaggcgg gaatctagaa cgtaaaatct aaagaaaccg
    13801 tgttgtaacg gcagaccgat gccgtcattc ccgcgtaggc gggaatctag gccattggac
    13861 agcggcaata ttcaaagatt atctgaaagt ccgagattct agattcccac tttcgtggga
    13921 atgacggaat gtaggttcgt gggaatgacg ggatttgaga ttgcggcatt tatcg
//
LOCUS       DAWFAN010000039        13685 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWFAN010000039 DAWFAN010000000
VERSION     DAWFAN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13685
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.039"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(5..408)
                     /locus_tag="WGO65_000816"
                     /pseudo
     CDS             complement(5..408)
                     /locus_tag="WGO65_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(565..3315)
                     /gene="gyrA"
                     /locus_tag="WGO65_000817"
     CDS             complement(565..3315)
                     /gene="gyrA"
                     /locus_tag="WGO65_000817"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ0327133.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            complement(3413..3913)
                     /gene="hscB"
                     /locus_tag="WGO65_000818"
     CDS             complement(3413..3913)
                     /gene="hscB"
                     /locus_tag="WGO65_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ0327134.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            3995..4216
                     /locus_tag="WGO65_000819"
     CDS             3995..4216
                     /locus_tag="WGO65_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ0327135.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            complement(4176..4496)
                     /gene="iscA"
                     /locus_tag="WGO65_000820"
     CDS             complement(4176..4496)
                     /gene="iscA"
                     /locus_tag="WGO65_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ0327136.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(4583..4969)
                     /gene="iscU"
                     /locus_tag="WGO65_000821"
     CDS             complement(4583..4969)
                     /gene="iscU"
                     /locus_tag="WGO65_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ0327137.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKGDDSVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            complement(5233..6447)
                     /locus_tag="WGO65_000822"
     CDS             complement(5233..6447)
                     /locus_tag="WGO65_000822"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ0327138.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            complement(6476..6922)
                     /gene="iscR"
                     /locus_tag="WGO65_000823"
     CDS             complement(6476..6922)
                     /gene="iscR"
                     /locus_tag="WGO65_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ0327139.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            7272..8444
                     /locus_tag="WGO65_000824"
     CDS             7272..8444
                     /locus_tag="WGO65_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ0327140.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            complement(8588..11368)
                     /locus_tag="WGO65_000825"
     CDS             complement(8588..11368)
                     /locus_tag="WGO65_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0327141.1"
                     /translation="MANNKTLFEVIENRKAVYLEDGDDEKCRLPEFVERNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHAKFLFTEKILQGDQVIPIRKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQGELDLEKEIND
                     RTSNAEIERKGWEHMVLELLLNKNGNPSENVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENIQADNFSFLTTFWASLNDSDNANEQGKTRTEQALKFMQDNK
                     FGFAHLANWVKQNYQKHNIIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFAANKDFRELLIWANKKIPNPNAKSNNADSLKANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLD
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARL
                     GDLKSQYDIHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVKDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(11358..13517)
                     /locus_tag="WGO65_000826"
     CDS             complement(11358..13517)
                     /locus_tag="WGO65_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0327142.1"
                     /translation="MKTDIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLSNDELKRHFFVKVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDSSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQSVSQSVSQSVSQSVSQLYTKL
                     TRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRYTADGKQAVGEIKRHSDGTPAENLII
                     KGNNLIALHSLAKQLKGKVKLIYIDPPYNTGEDSFKYNDKFSHSTWLTFMKNRLEIAK
                     DLLKDDGLIFVQCDDKEQAYLKILLDEIFTRENFINCIAVKMSEPSGNKMAHTSHRLP
                     KIKEYILIYKNINIKLNPIREQKSEWDDEYNIFLENFTQEDKRFIDLIISSQTENKEL
                     NADTLKEIDVLLKKISPISVNQKLAQLNIKDNEVIKWKLDNAYRIIRTAASSSVKKLA
                     DEKKEICQQQFFSVISKRDKLLYIVKSDYSKDAKAPRVQVLFAEDYLSISLCDLWTNI
                     NTTGLEAEGNVELKNGKKPESLIETIIKLATNENDIVLDYHLGSGTTAAVAHKMNRQY
                     IGIEQMDYIETLAVERMKKVIEGEQGGISKAVNWQGGGEFVYAELAPFNETAKQQILA
                     CENSDGIKMLFEGLCERYFLKYNVSVNEFGQIIQEPEFQSLPLDEQKQMVLEMLDLNQ
                     MYVSLSEMDDEQFAGCLNDDDKALSRAFYQAEKKDGE"
BASE COUNT         3140 a         2999 c         3326 g         4220 t
ORIGIN      
        1 ataactagga taaactcgat tttactaatt gttttaaaat ggaaatttga acttttatct
       61 cactgttgtt aaaacgccgt tcgtacccct ttaaatacag ctcaaaatgc gctttgggaa
      121 tgccgtcaaa cttgcgtaaa tgacgttttg cccggttcca aaagttctca attccattaa
      181 tatggttttg tcgttcagca aaataacttt catctgcttc tacttcgcca tcaaacattt
      241 ccaaatgcgg actgttttga taaataagta atcgtaaacg atgaaaataa taggctgcgg
      301 tacttttatt aacgcctact aactctgctg tcgttcttgc agttacacct gcgacaaaca
      361 gttcaatgag tttatttgtt tataccggct tagacgactt tttctcatag gggcaactct
      421 aacttaattt gaatttccct agttatctag gacagcccct tgtttttaat tgactataat
      481 ccgctatatt gtgagaagct ggatgacgga tgagggataa aataaatgcc gtctgaatct
      541 tcagacggca tcgggagatg attttcagtt ctcgacttcc ggttcggtta cattggaaat
      601 tacagaagcg tcggagagtt cggattcgtc ttcggcaaca cgttccagcg ataccaaggt
      661 ttcgccttcg tccaagttaa tcagtttcac gcctgctgcg gcgcggccgg tttcgcggat
      721 ttgttcgact ttggtgcgga taagtacgcc gccgctggta atcagcatca aatcgtcggt
      781 ttcgccgacc aaggttgcgg cgaccaaatc gccgtttcgc tcgccggtgt taatggcaat
      841 attgccttgc ccgcctttgt ttttgcggct gtaatcggca atcggggtgc gttttccgta
      901 tccgttggcg gtggcggtta aaacttgcaa accgctttct tcggtttcag gggcgaaggt
      961 aatcaggctg acgattttgc cgtcggcagg caggcgcata ccgcgcaaac cgccgctgcc
     1021 gcgaccggac gggcgaacac cgtgtttgcc gctcggcagt gcgttttcgc tgtcggcggt
     1081 ttcatcttcg atgccgtctg aaatttcggt ttcgatgtcg gcatcttccg cttcgtcgtt
     1141 gccggatttt tcccagtatt cgttgaagcg gatggcttta cctaagttgg agaacagcat
     1201 gatgtcgtcc gcaccgcctg tttgcgcagc gccgacgagg tagtcgcctt ctttgagcgc
     1261 gatggcttta atgccttggg cgcggacgtt tttaaaggcg gaaagttgga cttttttcac
     1321 cattccctgc gcggtggcga agaagacgta ttggtcttcg gggaactcgc gtactgccag
     1381 aatcgcgctg actttttcgc cttcttccaa ctggatgacg ttgttaatcg gacggccgcg
     1441 gctgttgcgt ccgccttcgg gcagtttgta aaccttaatc caatgacact tgcccaaatt
     1501 ggtaaagcac atcaaataat catgcgtgtt ggcaacaaac agggtttcga taaagtcttc
     1561 gtctttggtg gcagccgcct gtttgccgcg cccgccgcga cgctgcgcct gatagtcggt
     1621 ggtcggctgg gttttgatat agccgccatg tgtcagggta acgaccattt cgcgttgcgg
     1681 aatcaggtct tcatcggcaa tgtcgccgcc gaacgggttg atttcgctgc ggcgttcgtc
     1741 gccatagttg gttttgattt cttccagttc gtcgcggatg atttgggtaa tgcgttcggg
     1801 tttggagagg atatccacaa agtcgatgat tttacccatc aggtttttgt agctttcgac
     1861 aatttcttct tgatcgaggc cggtcaggtt tcgcaggctc atgcgtaaaa tagcatctgc
     1921 ctgaatctcg ctcaggtaat aaccttgctc tttcaagccg atgtttgcag ccaatccttc
     1981 cggacgcatc atttccaaat ccagaccgga acgcgtcagc atttcttcaa cgaggctgct
     2041 gcgccaaggg cgcgcaagca gtttgtcttt ggcctcggct gcgttgggcg attctttgat
     2101 gagcttgatg atttcatcga tattggacag tgcgacggct ttgccttcgg cgatatgccc
     2161 ttcatggcgt gccttcttca gccggaaaag cgtacgtcgg gtaacgactt cgcggcggtg
     2221 gcgcaggaat tcggagagaa tctgtttcag gttcaacagg cgcggttgtc cgtcgaccaa
     2281 aaccaccata ttgatgccga aactgtcttg cagcggagtc agtttgtaga gttggtttaa
     2341 gacgacttcg gcattttcgt tgcgtttcag ctcgataacg acgcgcatac cggatttgtc
     2401 ggattcgtcg cggagctcgg aaatgccttc cagtgttttt tcccgaacca aatcgccgat
     2461 tttctcgacc agcttggctt tgttgacctg ataggggatt tcgtcgataa cgatggcttc
     2521 gcgttcgccg tttctgccta tgggttcgat atgggtctta ccgcgcataa cgacgcggcc
     2581 gcggcctgtt ttatagcctt cgcgcacgcc gctcaagccg tagatggttg ccccggtcgg
     2641 gaagtcgggg gcttggataa tgtcgatcag ttcgtcgatt tcggtgtcgg gtgcatcgag
     2701 caggcgcagg caggcattga cggtatcgga aaggttgtgc ggcgggatat tggtcgccat
     2761 gccgacggcg atgccggacg agccgttgac gagcagtgtg gggaaacggg tcggcagtac
     2821 aagcggctcg tgttcgctac cgtcgtagtt cgggccgaaa ttgacggttt cttcctcaat
     2881 gtctgccagc atttcgtggg aaattttcgc catgcggatt tcggtgtagc gcatggctgc
     2941 ggcggcaagc ccgtccaccg atccgaagtt gccctgtccg tctatcagca cataacgcat
     3001 agcgaaattt tgcgccatac ggacgatggt gtcgtatacg gcggtatcgc cgtgggggtg
     3061 gtatttaccg atgacgtcgc cgacgatgcg cgccgatttt ttgtaggcgg cattccagtt
     3121 gtttttcaat tcgtgcatcg cgtacagtac gcggcggtgt accggcttga gaccgtcgcg
     3181 aacgtccggc agcgcacgcc cgacaatgac gctcatggcg tagtcgagat agcttttgcg
     3241 catttcgtct tcaaggctta ccggcagggt ttcgagggcg aatttgtggt cgtggcggat
     3301 ggttgcgtcg gtcatggttt caatgtttcg tatggcaaaa aattgttgct tattttagca
     3361 tattttgacg cggaacggtg cggcggttac gccgtctgaa acacggtgcg gattataatg
     3421 ccgaggaaat ttcgttgcgg agtttgtcga gaaacctgcc ttggcggact tgttgtgcgg
     3481 ctgtgtcgta atcttgtcgg gcaaacgact gtttcagacc gcagaacagt ttttcttgtt
     3541 cgtcgcggat ttcgttgtcg agatttttca aggattcaag gtcgttgcct gcccgtgcct
     3601 ccatcagcgt ttcgcgccat tccatttgct gcataaggaa ttcgggggcg aaagcggtat
     3661 gctccggcgc gtcggcatcg atgcccgatg ttttcagcag gtaggcggcg cggtcgatgg
     3721 ggtttttcaa ggtgcggtag gcatcgttga tggtggaaga catcatcact gcctgctttt
     3781 gctcaaaggc ggaagctgaa gcgaatttat cgggatggaa acgggcggcc aaggcgcggt
     3841 aggtttgttc caagttttcg gtgtcgatat cgaaagcggg ttcaatccgg aagagggtga
     3901 aatattggga catagtagga tgataaatgt aagattttgg cagaaaactg tttttgcctt
     3961 ataatctgcc gcttcttaaa cgaaaggact gaatatgggc ggcaaagtgc agcacaataa
     4021 aggcaaaata cgcgacaatg ctttaaaagc cttagtgaaa tccgatttgt tccggcacaa
     4081 ggtggaacgg aaaaggaaag gcaaaggcag ctacaacagg caggaagcga aaaaatggcg
     4141 ggacggtttt gatacggtcc cgccgttttt atgccttaaa cgtggaagct ttcgccgcag
     4201 ccgcaggagt ctttgacatt ggggttttca aatttgaaac cttcctgcaa accttctttg
     4261 gtgtaatcga cttgcgtgcc atccagataa accaggcttt tcggatcgat ataaatgcgc
     4321 gcgccgtgtc cttcgaaaat caggtcgtcg ccatcggctt cgtcgacaaa ttcaaggttg
     4381 tacgccatcc ccgagcagcc gctggttttc acacccaagc gtacgcccaa gcctttgccg
     4441 cgtttggcga gatagtcatt gatgtgtttt gcggcattct cggtaagggt aatcatattt
     4501 cttccttgtt gtaccgcccc ggacggacct gaagcggcgg gtggttcgga cggcattgcg
     4561 ggatgatgcc gtctgaaggg ctttatctgt tttcctgacg tttgcggtag tcggcaacgg
     4621 ccgcttttac cgcatcttca gccaagatgg agcagtggat ttttaccggc ggcaattcca
     4681 actcctcggc gatttcgctg tttttgattg ccagcgcgtc atccaggctt ttgcctttaa
     4741 cccactcggt aatcaggctg gacgaagcga tggccgagcc gcagccgtaa gttttaaatt
     4801 tcgcatcttc gatgatgccc tcgtcgttca ctttgatttg caggcgcatg acgtcgccgc
     4861 aggcgggcgc gccgaccatg ccggtgccga cggaatcgtc tcccttgtcg aatgtgccga
     4921 cgttgcgcgg attttcatag tggtcgatta ctttatcgct gtatgccatg atgtggtttc
     4981 cttaatgttt tttgatggtt taagtggttt gtttgccgat tttcagacgg cctgaagttt
     5041 agattttgca cgccccgcct tcgcagccgt cgtcgttttc cgaatcgacg ccgccttcca
     5101 cggtaacgcc gtcaaaatct tcaagcaggt tgtcgagatt gtccaaatct aaatcttgtt
     5161 cgttcatatt ttctcttgat tgctttgttt ttggaatgcc gtctaaaggt tcggacggca
     5221 tgttggtacg ctttaatgcg ctgcccattc aatcgagttc aaatcaatcc cgtctttgaa
     5281 catttcccac agcggcgaca gttcgcgcag tttgccgatt ttggatttaa tcagttcggc
     5341 ggcgaattgc acttcttctt cggtggtcat gcgaccgaag gtgatgcgca gggatgagtg
     5401 cgccagttca tcgttgcggc cgagcgcgcg caggacgtag ctgggttcga gactggcaga
     5461 agtacaggcg gagccgctgg atacggcgag ttctttcacc gccataatca ggctttcgcc
     5521 ttcgacgaag ttgaagctga cgttcaggtt gttcggggcg cggtgttcga ggtcgccgtt
     5581 gacatagact tcttcgatgc cttcgatgcc tttgaggaag atgtcgcgca gtttgcggta
     5641 gtgcgccata tcttgttcga gttcttcttt ggcgatgcgg aaggcttcgc ccataccgac
     5701 gatttggtgg gtcggcaatg tgccgctgcg gaagccgcgt tcgtggccgc cgccgtgcat
     5761 ttgggcttcg aggcggacgc ggggtttgcg gcggacgtac agcgcgccga tgcctttggg
     5821 gccgtacact ttgtgcgcgg acatcgagag caggtcgatt ttggcggctt cgacatcgac
     5881 aggcactttg ccgcatgctt gtgctgcgtc aacgtggaaa atgattttgc gttcgcggca
     5941 gatttcgccg atggcaggaa tatcttgaac cacgccgatt tcgttgttta cccacattac
     6001 ggaaacgagg atggtgtctt cgcggatggc ggctttcagt acgtctaaat caaccaaacc
     6061 gttttcttgt acgtccagat aagttacttc gtaaccttgg cgttcgagtt cgcgcatggt
     6121 gtcgagtacg gctttgtgtt cggtttttac agtgatgagg tgattacctt tagatttgta
     6181 gaagtgcgcc gcgcctttga tagcgaggtt gttggactcg gttgcgccgc tggtgaaaac
     6241 gatttcttta gagtcggcgt taatcagggc ggcaatgtct gcacgtgctt tttctacagc
     6301 ttcttctgct tcccagccga agctgtggct gttggaggct gggttgccga aggtttcggt
     6361 cagataggga atcatttttt cggcaacgcg tttgtcaacg ggggtggtgg cggcgtagtc
     6421 gaggtaaacg ggggttttga cggtcatggt ttgctctttc tttttcgggt gttatttaat
     6481 ggatgtgtgt aaattggacg acgcggctgc cgtcgccgtt gtttttctgt tcgatgatgc
     6541 tttgcagggt aacgctgccg aggtagtcgt tgatggtttt gtttaaattc tcccaaagat
     6601 cgtgcgtcag gcagggcgcg ccgtggtggc agttggcttt gctgccgcat tgggtcgcgt
     6661 ccagccggtc ttcggcggcg gcgatgattt gggcgatgtt gatgcgtgcc gccggtgcgg
     6721 cgaggatgta gccgccgccg ggcccgcgca ggctttcaac cagtccggcg cggcggagtt
     6781 tgccgaacaa ttgctcgaga taggagaggg atatgttttg gcgttcgctg atggcactga
     6841 gtttgacggc gccggtttgc gcgttcatcg ccaaatccag catagcggta acggcaaaac
     6901 gccctttggt ggtcagtctc atggtggtgg ttgcccgtgt cggctttttt atagtgggga
     6961 gaattgtcca atatctgagt gtttcagtca agtaaggggg agctgttgcc gattgtttgt
     7021 ttaaatggcg gtatagtctg attttgcttt atttgtttgg ttttttaaat cagttggtta
     7081 tgttttttga ggagcggtgg tcggggtatt ttgccggcaa gcggtttcaa aacgatttga
     7141 tttatttttg atattgatat agattatcta aatcttagtg atttatttgg gtattgcctg
     7201 aaatgctgtc tgaaggctca agcggtttcg gacggcatat tccatcaggc tgtcaaacag
     7261 ggaaaaacaa gatgaaacgt gatttgagca aaatgacctg catcgaagac ttgcgccgcg
     7321 ttgccaagcg caaaatgccg cgtatgtttt acgattacat cgattcgggt tcgtggacgg
     7381 aaactaccta ccgcgaaaac acttcggatt ttaaagatat ccgcttccga caaaaggtat
     7441 tggtcaatat ggaaggtcga agcctggaaa ccaaaatgat cgggcaggat gtgaaaatgc
     7501 cggtggcgat tgcgccgacg ggttttaccg gtatggcaca cgccgacggt gaaatcttgg
     7561 cggcgcgggc ggcggagaag ttcggcattc cgtttacgtt gtccacgatg tctatctgct
     7621 cgattgaaga cgttgccgag aacacttccg caccattttg gtttcagctt tatgtgatgc
     7681 gcgaccgcga gtttatggaa aacctgatta agcgcgcgaa ggatgccaaa tgttcggcat
     7741 tggtattgac cgccgatttg caggttttgg gtcaacgcca caaagacatc aaaaacggcc
     7801 tgtccgcgcc gccgaaaccg accatcgcca atttaatcaa tctggcgacc aaaccggaat
     7861 ggtgcatgaa aatgctgaac acggaacgcc gcacgttccg caatatcgtc ggacacgcga
     7921 agaacgtcgg cgatttgtct tcgctgtctt catggacttc cgaacaattc gacccgcgcc
     7981 tgagctggga cgatgtggcg cgcattaaag atttgtgggg cggcaagctg attatcaaag
     8041 gcattatgga acccgaagac gcggaaaaag cagcgaaaag cggcgcggac gcattgattg
     8101 tttccaacca cggcggccga cagctcgacg ataccgtgtc tgccatcaaa gccttgcccg
     8161 acatcgtcag cgcagtgggc agcgacatcg aagtttggat ggacagcggc atccgcagcg
     8221 gtcaggatat tttgaaggcg tgggctttgg gcgcgaaagg tacaatgata ggtcgggcgt
     8281 ttctgtatgg tttgggtgcg tatggtgaag aaggcgtaac ccgtgcgctg gaaatcctgt
     8341 ataaagaaat ggatatatct atggcgttta cgggacaccg cgatattcaa gatgtcgatg
     8401 cttcgatttt gagaggcaaa gattggggca gggaaacggt ttagatactg aaaaatacga
     8461 tgccgtctga acacagggtt cggacggcat taaaatttgg ctgtatttta ataatgtgtt
     8521 gattaaatat ctgacaaaac gcccttttgt aaaggaagtt ttgtcaaacg gggttgcctg
     8581 caatgcacta cttcattttt tcaagcgatg ccgcacctaa agggaatgac tccgtaaatt
     8641 gtccattttt ctgatgatca gtaaaaaacg gtaaaccgat caaacgataa tgcggtgtat
     8701 ctttttgcag gatcttatcc atcccatatt ccacagtgat ggcttctaaa aattcttctt
     8761 tccatccatc agtttccact aaatgctcac ctttgggctc aatgaaaatt tggtaatgca
     8821 aaaagtcctt cacgccattg gaagaagatt tttgtttatc tttcagcaat aaaataaagt
     8881 ccggcataaa tccttcacca tcggcaaagt tattcaattt aaacacttct tcattacgga
     8941 ttaaatgaat gtcatattga gatttcaaat cgcccaaacg cgctgaaata aattgaatta
     9001 acgcttcttc caaacccgtt ccggcaaaat tattcatcac ataccaatca ttttgcgtgg
     9061 caatagtcgt ttttacatca tcttttttga cccatttttg ttttggcgtg ccgaaaattt
     9121 cccataattt tttaggtgta aattcttttg taccgataaa cggcatatca ctttcattca
     9181 gatgtttttc aaccatttcc aagattttca ggcagcctgc aagtttatcg tccaggcgaa
     9241 cctgtttgtc ttgccctaat cctaaaaatt caatttgcca gtctttcaac aagttatttt
     9301 gcaattcatt gcgattttga atgtcgagct tgctttgcaa acggttgaaa tggaataaag
     9361 attgactatt tttccctttg atatgtaatg ctttattgaa aatgtgccgc tccatttcac
     9421 tcatttttat ggtttgagta aaattatttt gtgtgccgat ttgtcgggct gtttcatcat
     9481 tttcatcggc tgtaaattgc gtttcctgca acagttgatt accgtgaact tggaatgaaa
     9541 gcgtttgtgg attggctttt aggctgtctg cattattgct tttggcgttt gggttgggga
     9601 tttttttatt cgcccaaatt aacaattctc taaaatcctt attagcggca aattcagatt
     9661 tgagtttaaa tgttgccaat accttatcat cgtctttttc aggcaaataa ccgtcttttc
     9721 gcaactcatt tttcagttct gaaatatagc gggattgctc atcgtgcgtg taataaaaca
     9781 attcttccaa gatacgcaat tcgtgttgca tgtcgttgtc aaatttgcgt ttattcggct
     9841 gtttaccttc aaacgcaaat ggaaaataac gcacgccacg accaatcaac tgcttttccg
     9901 atacggtagc ggcagccgtt ttgcctgatt ttttatttga accaccgcca ttttgccctt
     9961 catacaaacg cacaatatca aacaaattca gaacgtccca accttcagtt aatctatcca
    10021 ccgtaaaaat ggcacggatc ggattatcag ccgcttccaa attattcagc aatttttccg
    10081 tttcgctgtc ggttttttcg gttttggttt tgttggtttc ggagttggta ataatgatat
    10141 tgtgtttttg ataattttgt tttacccaat ttgctaaatg tgcaaaccca aatttattgt
    10201 cctgcataaa ttttagggct tgttcggtgc gggttttacc ttgttcgttg gcgttatcgc
    10261 tatcgtttaa gcttgcccaa aatgtcgtta aaaacgaaaa attatccgcc tgtatatttt
    10321 ctgcccaatt taaaaacgcc gaataatccg cttttgattc atcaatcgtc ttacttctaa
    10381 acaacatcac aggcttgaaa ttggcaatgc cgtatttcaa cgcgatttga tggcgatacc
    10441 aagcaaacaa taaagcgtgc aacacccgct ctttcttgcc cagcgtactg gataccaaat
    10501 tgatttcttt ggtataacct ttttgcaaaa actcttttaa gccaaatttt gtgatgattt
    10561 tatccgcgta tttttgttgc acgtcagcat tttcaggtag cgtggcggta aattccaaca
    10621 gcacattttc gctaggattg ccatttttat tgagtaacaa ttccaaaacc atatgctccc
    10681 agcctttgcg ttcaatctcg gcattgctag tgcggtcgtt aatttccttt tctaaatcta
    10741 attcgccttg ttttttgcct ttggtttgtg cgtttaaatg gtgtgcttca tcgcccagca
    10801 tcacaaggtt caatttgtgc aaatccgcca atgtggtttg gttttcccgt tcggtgtgga
    10861 tatcgttata cagtttttga atactggtaa atttaatttc aatgccgtct gaatgtgggc
    10921 taaacgtttc tactttgcga atgggaataa cttggtcgcc ttgcaaaatc ttctcggtaa
    10981 ataaaaattt tgcgtgcgcc gggtcaataa aattattttc cgttttatcc acgatgttat
    11041 tttgattaac gaaaaacaaa aaatgccgat aacccttttc aaaataatac aaaatcaacg
    11101 ccgccatcat catcgttttg cctgcacctg ttgccatatt gaacagcaaa tgggtagggc
    11161 ggtttttgat gtcagggaaa tcctttaatt ttgaagtgcg gtcaaaaatc acaaaatttt
    11221 ctaaagccga tttttgccat tcaaaaaacg gatattttaa attgcgttct acaaattcag
    11281 gcagtctgca tttttcatca tcgccgtctt ccaaatacac agctttgcgg ttttcaatca
    11341 cttcaaacaa agttttatta ttcgccatct tttttctccg cttgatagaa cgcacggctt
    11401 aaggctttat catcatcatt caagcagcct gcaaattgtt catcgtccat ttcggataat
    11461 gaaacataca tttgatttaa atccaacatt tcaagcacca tttgtttttg ttcgtctaat
    11521 ggcaaagatt gaaattcagg ctcttgaatg atttgaccaa actcatttac gctcacattg
    11581 tattttagga aatagcgttc gcataaacct tcaaacagca ttttgatgcc gtctgaattt
    11641 tcgcaagcca aaatttgttg ttttgcggtt tcgttaaatg gagcaagctc ggcatagaca
    11701 aactcgccac caccttgcca attcacggct ttggaaatac caccttgctc gccttcaatc
    11761 acttttttca tgcgttcaac ggcaagcgtt tcaatataat ccatttgttc aataccaata
    11821 tattggcggt tcattttgtg agcaacggca gcggttgtgc cactacctaa atggtagtct
    11881 aggacgatgt cgttttcgtt ggttgctagt ttaataatag tttcaattaa actttctggc
    11941 tttttaccat tttttaactc tacatttcct tctgcttcta gtcctgttgt atttatattt
    12001 gtccataaat cgcataaact aatactcaaa taatcttctg caaacaaaac ctgcactcgt
    12061 ggtgcttttg catcttttga gtaatctgac ttaacgatat acaataattt atctcgttta
    12121 gaaataactg aaaaaaattg ttgttggcaa atttcctttt tttcatctgc taattttttt
    12181 acgctactac tagctgctgt tcttataatt ctatatgcat tatctaattt ccattttata
    12241 acttcattat ctttaatatt taattgggct aatttttgat taactgaaat aggagatatt
    12301 ttctttaaaa gaacatcaat ttcttttaaa gtatcagcat tcagttcttt attttctgtt
    12361 tggctactta ttattaaatc aataaatctt ttatcttctt gtgtaaaatt ctctaaaaaa
    12421 atattgtact catcgtccca ttctgatttt tgctctctaa tagggtttaa ttttatattt
    12481 atatttttat agataagaat atattccttt atttttggta gtctatgcga tgtatgcgcc
    12541 attttgttac ctgatggctc tgacattttt acagcaatac agtttataaa attttctctt
    12601 gtgaaaattt catctaataa aatttttaaa taagcctgtt ccttatcatc gcattgaaca
    12661 aagattagcc catcatcttt taataaatct ttcgcaattt ctaaacgatt tttcataaaa
    12721 gtaagccaag tggaatgact gaatttgtcg ttatatttaa aactgtcttc ccctgtattg
    12781 tatggcgggt caatataaat cagcttcact ttacctttta actgtttggc aagcgaatgc
    12841 agggcaatca gattattgcc tttgataata agattttcgg cgggtgtgcc gtctgaatgt
    12901 cgtttgattt ctccaacggc ttgtttgccg tctgcggtat agcgagagaa ttttgagaat
    12961 gcttttgcgt caaaaagccg atcgatttca tcaaaagcaa gcgtttgatt aaaaaagatt
    13021 tcttgtctct ttcgggttaa tttggtgtat aattgactga ctgactgact gactgactga
    13081 ctgactgact gactgactga ctgactatta ttgcgtttaa aataaatctc ttcgccttct
    13141 tcggtgcttt gtccgccatt caatacgcaa tctttaaacg gaaaatccaa cacaatatcc
    13201 gaactgtctt tcaaaaagcg gttgccgtcc gtcaaaccaa tgcggttggc gtattttgtg
    13261 taggaattat tgacgctgtg tttgtccaag aaaaaacgga aatcctgcaa tttaaacacc
    13321 agcacaccat tcactttcac aaagaaatgg cgttttaact catcattact caataacaaa
    13381 ccgataatgg tcgggtcggt tttttccacc aaatccaaca gaatgttttt ggctaaaatg
    13441 gttttttctt cgttcgccca tactttttcg tgtgatagca gggcttgggt taattcggtt
    13501 tgaatgtctg tcttcatcgt tataaatcct catcagttat tttaatgtgc cattcttgtg
    13561 taacggcaaa tggctttgca aataaattct aataaaatca atcctttgat gtctttgctg
    13621 tttccggggg cgttcgcaaa gttgcgataa gcggggcggg tgtccgagtg tgtcggaatt
    13681 ttgcg
//
LOCUS       DAWFAN010000040        13538 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWFAN010000040 DAWFAN010000000
VERSION     DAWFAN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13538
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.040"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..79)
                     /locus_tag="WGO65_000827"
     CDS             complement(<1..79)
                     /locus_tag="WGO65_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0327143.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WGO65_000828"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WGO65_000828"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ0327144.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPDLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERIRFTTSHPREFTDAIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMVGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WGO65_000829"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WGO65_000829"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ0327145.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVVDMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDGLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WGO65_000830"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WGO65_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ0327146.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQGLSQRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDSLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4804..5868
                     /locus_tag="WGO65_000831"
     CDS             4804..5868
                     /locus_tag="WGO65_000831"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ0327147.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYIAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            5888..6847
                     /locus_tag="WGO65_000832"
     CDS             5888..6847
                     /locus_tag="WGO65_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ0327148.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKSLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPSGKAEYHG
                     KAFSSDDPNGRLHYSIDFTKKQGYGRIEHLKTPEQNVELASAELKADEKSHAVILGDT
                     RYGGEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            6986..7663
                     /gene="tsaB"
                     /locus_tag="WGO65_000833"
     CDS             6986..7663
                     /gene="tsaB"
                     /locus_tag="WGO65_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ0327149.1"
                     /translation="MQADFNRPVLAIDTGTSYLSLALRADGETRLFHQEVGSRQSELI
                     LPEIRTLFHDAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASMPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADITLPEGHVFSDG
                     IGNAFALENRPPFAGKPDMPTAADFLNLAEEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            7660..8100
                     /gene="rimI"
                     /locus_tag="WGO65_000834"
     CDS             7660..8100
                     /gene="rimI"
                     /locus_tag="WGO65_000834"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ0327150.1"
                     /translation="MNIRRPSPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRLAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEGTQRLLLE
                     VRAGNIGAQTLYAAHGFSIAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8094..8831
                     /locus_tag="WGO65_000835"
     CDS             8094..8831
                     /locus_tag="WGO65_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ0327151.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8893..9534
                     /gene="pyrE"
                     /locus_tag="WGO65_000836"
     CDS             8893..9534
                     /gene="pyrE"
                     /locus_tag="WGO65_000836"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ0327152.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9608..10042
                     /locus_tag="WGO65_000837"
     CDS             9608..10042
                     /locus_tag="WGO65_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327153.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10039..11349
                     /gene="argA"
                     /locus_tag="WGO65_000838"
     CDS             10039..11349
                     /gene="argA"
                     /locus_tag="WGO65_000838"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ0327154.1"
                     /translation="MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFHLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AVTLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFTEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11445..13460
                     /locus_tag="WGO65_000839"
     CDS             11445..13460
                     /locus_tag="WGO65_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ0327155.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARTLS
                     DGILAQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCAAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPDRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPADCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFTDGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGASWTDEYGNPQKYEVCKCRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
BASE COUNT         3021 a         4223 c         3602 g         2692 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccaaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatac agccgtcctg
      121 accgattaaa ttcatatttt aaaacaatat cctgcctcca aaacccacat cgtgctataa
      181 tccacaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gtaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcacagg aaaaagtgtt ctccgacttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaat cccgacctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtcgtcttc ggcccgcaaa
      601 cgctgcaccg cctgccaaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgacc gaaatcgcca
      841 accttgccca gcaaggtgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgt atcgtccacg
      961 aaatccccgg catcgaacgt atacgcttca ccaccagcca tccgcgcgaa tttaccgacg
     1021 caattatcga gtgctaccgc gacctgccca agctggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgcgccatcc gtcctgattt gtgcctcagc tccgacttta
     1201 tcgtcggctt ccctggcgag accgagcgcg agttcgagca aaccttgaaa ctggtcaaag
     1261 acatcgcctt cgacttgagc ttcgtcttta tttacagtcc gcgccctggc acgcctgccg
     1321 ccaacctgca cgacgacaca ccgcatgaag aaaaagtgcg ccgcctcgaa gctttgaacg
     1381 aagtcatcga agccgaaacc gcgcgtatca accaaaccat ggtcggcacg gttcaacgct
     1441 gcttggtcga aggtatctcc aaaaaagacc ctgaccaact gcaagcccgt accgccaaca
     1501 accgcgtggt caactttacc ggcacacccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaaattgt cgaagcctaa accctcacgc
     1621 cgaaaaaatg ccgtctgcag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa acccaaaccg tccaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacaaagcgc
     1981 atatcgacga cggtggcgtt cagttttccg gcgaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttcccacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg catccgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggc
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catgccttcg cacgccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caatgccgac atcgaaataa cggtcgggga atcgttgttc aaactccacc
     2521 agtccgctgc cctcgcgcat cgcgggcgtg atggcgatca aacgctcgtc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcgac gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgcct tctttaggca gcttggcgac ggcgtggtat
     2701 ttgacggggt cgttttcagc gagtttgtag ccgttgcctt ttttggtgat gacgtgcaga
     2761 agctgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt gtagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatgcccaa cgccgcgccg
     3241 atggatgtgg aagaatgccc tacgccgaac gcgtcgtatt ccgactcgct gcgttttggg
     3301 aagccagcta aaccgccgta ttggcgcatg gtgtgcatct ggtttttcct gcctgtcagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccatacca gcttgtcttc gggcgtatcg
     3421 tacacatagt gcagggcgat ggtcagttcg accgcgccca aattgctggc gaaatgcccg
     3481 ccggtctgcc cgacagattc cagcagaaag gcgcgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggccaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gcttggtata aatctgcgtg
     3781 gtcgaaaggc tgctgtgccc gagcagctcc tgcaccgccc tgatgtcgcg cgaagcctgc
     3841 aacaggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccggtcagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 gtaacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagt tcgaacagcg cgtgatcgcg caccgccagc
     4201 gagtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagggct
     4261 ttgggtacgc gctcgggctg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc
     4321 agaccgcgtt tgaccagcca ggcgcaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgttgc
     4441 gacaagccct gcggcacgcc gccttcagct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgcc accgtgtgct ccgacttgcc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca
     4621 cacaaatatc aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc
     4681 gcccaaaatc cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta
     4741 tggttttttg ccgcaaaaaa cattataatc cgccttattt acctattgcc caaggagaca
     4801 caaatggcac tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc
     4861 ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac
     4921 caagtcaacg cgcccgtcat cgtacaggcg agtgcaggtg cgcgcaaata tgcgggcgcg
     4981 ccgtttttgc gccacctgat tctggcggct gtcgaagaat ttccgcacat ccccgtcgtc
     5041 atgcaccaag accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttc
     5101 tcctccgtga tgatggacgg ctcgctgctc gaagacggca aaaccccttc ttcttacgaa
     5161 tacaacgtca acgccacccg taccgtggtt aatttctccc acgcctgcgg cgtatccgtt
     5221 gaaggcgaaa tcggcgtatt gggcaacctc gaaaccggcg aagcaggcga agaagacggt
     5281 gtaggcgcag tgggcaaact ttcccacgac caaatgctga ccagcgtcga agatgccgtg
     5341 cgcttcgtca aagacaccgg cgtggatgcg ctcgccatcg ccgtcggcac cagccatggc
     5401 gcatacaaat tcacccgtcc gcccacaggc gacgtattac gtatcgaccg catcaaagaa
     5461 atccaccaag ccctgcccaa tacacacatc gtgatgcacg gctccagctc cgttccgcaa
     5521 gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg
     5581 gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgta aagtcaacat cgataccgac
     5641 ttgcgccttg cttccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttc
     5701 gatccgcgca aatatttgag caaaaccatt gaagcgatga agcaaatctg cctcgaccgc
     5761 tacatcgcgt tcggttgcga aggtcaggca ggcaaaatca aaccggtttc cttggaaaaa
     5821 atggcaaacc gttatgccaa gggcgaattg aaccaaatcg tcaaataaca ggttgcttgt
     5881 aaacaaaatg ccgtctgaac cgttgttcgg acggcatttg atttttgctt ctttgacctg
     5941 cctcattgat gcagtatgca aaaaaagata ccataaccaa aatgtttata tattatctat
     6001 tctgcgtatg actaggagca aacctgtgaa ccgaactgcc ttctgctgcc tttctttgac
     6061 cgccgccctg attctgaccg cctgcagcag cggaggcggc ggtgtcgccg ccgacatcgg
     6121 cgcggggctt gccgatgcac taaccgcacc gctcgaccat aaagacaaaa gtttgcagtc
     6181 tttgacgctg gatcagtccg tcaggaaaaa cgagaaactg aagctggcgg cacaaggtgc
     6241 ggaaaaaact tatggaaacg gcgacagcct caatacgggc aaattgaaga acgacaaggt
     6301 cagccgcttc gactttatcc gtcaaatcga agtggacggg cagctcatta ccttggagag
     6361 cggagagttc caaatataca aacaggacca ctccgccgtc gttgccctac agattgaaaa
     6421 aatcaacaac cccgacaaaa tcgacagcct gataaaccaa cgctccttcc ttgtcagcgg
     6481 tttgggtgga gaacataccg ccttcaacca actgcccagc ggcaaagccg agtatcacgg
     6541 caaagcattc agctccgacg acccgaacgg caggctgcac tactccattg attttaccaa
     6601 aaaacagggt tacggcagaa tcgaacacct gaaaacgccc gagcagaatg tcgagcttgc
     6661 ctccgccgaa ctcaaagcag atgaaaaatc acacgccgtc attttgggcg acacgcgcta
     6721 cggcggcgaa gaaaaaggca cttaccacct cgcccttttc ggcgaccgcg cccaagaaat
     6781 cgccggctcg gcaaccgtga agataaggga aaaggttcac gaaatcggca tcgccggcaa
     6841 acagtagcat ttgaaaaatg ccgtccgaac acgatagttt accgttcgga cggcattttt
     6901 gcgccatact gccctaggac acgacggcac ggcaggcaag ctgatttcac tatataatgc
     6961 cgtctgaacc aacgagaacc ataccatgca agctgatttt aaccgtcccg tcctggccat
     7021 cgataccggt acttcctatt tgtcgctcgc gctgcgcgcc gacggcgaaa cccgtctgtt
     7081 ccatcaggaa gtcggcagcc gccagtccga actgattctg ccggaaatcc gcacgttgtt
     7141 tcacgatgca ggcattaccg ccgccgattt gggggcgatt gtttatgcga aaggtcccgg
     7201 cgcgtttacg ggactgcgta tcggcatcgg cgtggcgcaa ggtttggcaa cgccgtttga
     7261 tacgccttta atcggcatcc ccacgctcga tgccgccgcc tcgatgccgc cgccgcaaag
     7321 ctgcatactc gccgctgcgg acgcgcgtat gggcgaagtg ttttatgcgt ggttcgacac
     7381 gctgaaccgc cgccgtttga gcgattatca agtcggtagg gcggcagaca ttaccctccc
     7441 cgagggacac gtcttttcag acggcatcgg caatgcgttc gcgctggaaa accgtcctcc
     7501 cttcgcaggt aaacccgata tgccgactgc cgccgacttt ctcaatctgg cggaagaagg
     7561 cggttttccc gccgtcgctg cggcacacgc cgaactgctc tacgtccgca acaaaatcgc
     7621 cttgactgcc aaagaacagg cggaacggaa agcccgaaca tgaacatccg ccgcccctca
     7681 ccgtccgact gcgccgcact cgccgcgctc gatgccgtct gcaacccgtc cgcgtggacg
     7741 caacgccaat ttgagtccgc actggtttcg ccgtccgaac aggttttcct tgcggaaaaa
     7801 gacggcaggt tggccgcctt tatcgtttgg cagaacctgc ccgacgaatc cgaactgcac
     7861 ctgattgcca ccgcgcccga atgccgccgc cggggtgttg cgtccgccct gctcgaatac
     7921 tggttcgcac atctgcccga aggcacgcaa cgcctgctgc tcgaagtccg cgcgggcaat
     7981 atcggcgcgc agacgctgta cgcggcgcac ggcttcagca ttgcgggcag gcggaaaaac
     8041 tattaccgta cagccgacgg taaaaccgaa gatgccgtct tgatggagaa aatatgttaa
     8101 gcgcgcgcta cctccacctg cacgaagctt tgggtttagg cccgatgtgg ctgaaacagg
     8161 ccgccgccgt cctgccgccc aaaaacacac ccgcaacccc ggcacaggca cgtcctcaaa
     8221 ccgtccgcgc cgccccgatc cgcccttccc aaccccataa cggtcaggcg cggctcgaaa
     8281 cgatgaaagc gttggaaacc gccgccgtac ctacgcgcaa acccgcgcct gaaaccgaaa
     8341 cgcctctgcc cggcctttca gacggcatcg cccccgttcc cgccgcttcg ggcatcacca
     8401 agcttgccgt cgtcagcctt tgcccaccga tcgaggatgc ggtttacggg caactgttct
     8461 acggcaaagc gggtgtcctg cttgacaata tactcaaagc cgtaggactg gatgccgcct
     8521 atgtccacaa aacctgttgg gtgaaaaccg ccgccgtcgg caacccgatg ccgtctgaag
     8581 cggcaatcga aaccgcgacg gtttccgtca tgcaggaact cgacggctgc cgcgcccccg
     8641 ccgtcctatt cctcggacag gcttttgtcc agcctgaacg gcaaacgatg attgaaactt
     8701 tgtgcggcag ccgtcccttc ttcatcatcg accatcccgc ccggctgttg cgccaacccg
     8761 aacttaaagc ccgcgcctgg caggtgttga aacagttgaa acgcgccttg cggcaaggcg
     8821 gcggcagttg aagcgcaccg cacggggcgg tagaatcgca actgcgtccc aatatctgac
     8881 agaaagcaca aaatgaccga tttccgccaa gatttcctca aattctccct cgcccaaaat
     8941 gttttgaaat tcggcgaatt taccaccaaa gccggacggc ggtcgcccta tttcttcaat
     9001 gccggcctct ttaacgacgg cttgtccacg ctgcaactgg caaaatttta cgcacaatcc
     9061 atcattgaaa gcggcatccg attcgatatg ctgttcggcc ccgcctacaa aggcattatt
     9121 ttggcggcgg caaccgcgat gatgctggcg gaaaaaggcg tgaacgtccc gtttgcctac
     9181 aaccgcaaag aagccaaaga ccacggcgaa ggcggcgtgt tggtcggtgc gccgcttaaa
     9241 ggccgcgtgc tgattatcga cgacgtgatt tccgccggca catccgtacg cgaatcgatc
     9301 aagctgattg aagcggaggg cgcaaccccc gccggtgtcg ccatcgcgct cgatcgcatg
     9361 gaaaaaggca cgggtgagtt gagcgcggtt caggaagtgg aaaaacaata cggtctgccc
     9421 gtcgccccca tcgccagcct gaacgatttg tttattctgt tgcaaaacaa ccccgaattc
     9481 ggacagttcc tcgaacccgt ccgagcctac cgtcggcagt acggcgtaga ataaaaacaa
     9541 agcatatgcc gtccgaaccg ccttacgcct cagacggcat caaacctgac acacacgagg
     9601 aaataccatg cccgcctgtt tctgccccca ctgcaaaacc cgtctctggg tcaaagaaac
     9661 ccaactcaat gtcgcccaag gcttcgtcgt ctgccaaaaa tgcgaaggac tgtttaaagc
     9721 caaagaccat ctggcaagca cgaaagaacc catattcaac gatttgcccg aggctgtttc
     9781 ggatgtcaaa ctcgttcacc gtatcggcac gcgcgccatc ggcaagaaac agatttcccg
     9841 tgacgaaatc gccggcatcc tcaacggcgg cacaacccag cccgatattc cgcccgcaac
     9901 cgccgccacc cctgctgccg caccgcaggt taccgtaccg cccgccgcgc ccgcccgtca
     9961 ggatgggttc aactggacga ttgcaaccct gtttgccctt atcgtcctca ttatgcagct
    10021 ttcctacctc gtcatcctat gatcgtgccc gacctctttg tcgcccactt ccgcgaagcc
    10081 gccccctaca tccgccaaat gcgcggcaaa acgctggtcg ccggcataga cgaccgcctg
    10141 ctcgaaggtg ataccttaaa caagctcgcc gccgacatcg ggcttttgtc gcaactgggc
    10201 atcaggctcg tcctcatcca cggcgcgcgc cacttcctcg accgccacgc cgccgcgcaa
    10261 ggccgcacgc cgcattattg ccggggcttg cgcgttaccg acgaaacctc gctcgaacag
    10321 gcgcagcagt ttgccggcac cgtccgcagc cgttttgaag ccgcattgtg cggcagcgtt
    10381 tccgggttcg cgcgcgcgcc ttccgtcccg ctcgtatcgg gcaacttcct gaccgcccgt
    10441 ccgataggtg tgattgacgg aaccgatatg gaatacgcgg gcgttatccg caaaaccgac
    10501 accgccgccc tccgtttcca actcgacgcg ggcaatatcg tctggctgcc gccgctcgga
    10561 cattcctaca gcggcaagac cttccatctc gatatgcttc aaaccgccgc ctccgtcgcc
    10621 gtctcgcttc aggccgaaaa actcgtttac ctgacccttt cagacggcat ttcccgcccc
    10681 gacggcacgc tcgccgtaac cctctcggca caggaagcgc aatcgctggc ggaacacgcc
    10741 ggcggcgaaa cgcgacggct gatttcgtcc gccgttgccg cgctcgaagg cggcgtgcat
    10801 cgcgtccaaa tcctcaacgg tgccgccgac ggcagcctgc tgcaagaact cttcactcgc
    10861 aacggcatcg gcacgtccat tgccaaagaa gccttcgtct ccatccggca ggcgcacagc
    10921 ggcgacatcc cgcacattgc cgccctcatc cgcccgctgg aagaacaagg catcctgctg
    10981 caccgcagcc gcgaatacct cgaaaaccac atttccgaat tttccatcct cgaacacgac
    11041 ggcaacctgt acggttgcgc cgccctaaaa acctttaccg aagccgattg cggcgaaatc
    11101 gcctgccttg ccgtctcgcc gcaggcacag gacggcggct acggcgaacg cctgcttgcc
    11161 cacattatcg ataaggcgcg cggcataggc ataagcaggc tgttcgcact gtccacaaat
    11221 accggcgaat ggtttgccga acgcggcttt cagacggcat cggaagacga gttgcccgaa
    11281 acgcggcgca aagactaccg cagcaacgga cgaaactcgc atattctggt acgtcgcctg
    11341 caccgctgac cgcaacggaa agccgccgca gaaatgccgt ctgaaccccg tttcagacgg
    11401 catttccccg attataaaga cctgcccaac cctcaaggac cccgatgaaa tcctaccccg
    11461 acccctaccg ccattttgaa aacctcgatt ccgccgaaac gcaaaacttc gctgctgaag
    11521 cgaatgccga aacgcgcgcg cgttttttag aaaacgacaa ggcgcgcacg ctgtcggacg
    11581 gcattttggc gcagatgcag gacacgcggc agattccgtt ttgtcaggaa caccgcgcgc
    11641 ggatgtacca tttccatcag gacgcggagt atccgaaggg cgtgtaccgc gtgtgtgcgg
    11701 cggcgacgta tcgttccggc tatcccgagt ggaaaatcct gttttcagtg gcggatttcg
    11761 acgagctgct cggcgacgat gtgtatttgg gcggcgtgtc gcacttggtg gaaaagcccg
    11821 accgtgcgtt gttaacactg agcaaatcgg gcggcgatac ggcgtacacg ctggaagtgg
    11881 atttggaagc aggggagttg gtagaaggcg gttttcactt tccggcaggc aaaaaccatg
    11941 tgtcgtggcg cgatgaaaac agcgtgtggg tgtgtccggc ctgggacgaa cgccagttga
    12001 ccgaatcggg ctatccgcgc gaggtgtggc tggtggaacg cggcaaaagt ttcgaggaaa
    12061 gcctgccggt gtaccaaatc ggcgaagacg ggatgatggt gaacgcgtgg cgttacctcg
    12121 atccgcaggg ttcgccgatt gatttgattg aagcgtctga cggtttttac accaaaacct
    12181 atttgcaggt gtcggccgaa ggcgaggcga aaccgttaaa cctgcctgct gattgcgatg
    12241 tggtcggcta tctggcgggg catcttttgc tgacgctgcg caaggactgg caccgcgcga
    12301 accaaagcta tccgagcggc gcgctggtag cggtgaaact gaatcgcggc gaaatcgggg
    12361 cggcgcagct tttgtttgcg cctgatgaaa cgcaggcatt ggaaagcgtg gaaacgacca
    12421 agcgttttgt cgtggcgagt ctgctggaaa acgtacaagg ccgtctgaaa gcgtggcgtt
    12481 ttaccgacgg caaatggcag gaagccgaac tgccgcgcct gccctcgggc gcgttggaaa
    12541 tgaccgacca accgtggggt ggcgacgtgg tttaccttgc cgccagcgat ttcaccacgc
    12601 cgctgacgct gtttgcgctg gatttgaacg tgatggaact gaccgtcatg cgccgtcagc
    12661 cgcagcagtt cgattcagac ggcatcaacg tgcagcagtt ttggacgact tcggccgacg
    12721 gcgagcgtat tccttatttt cacgtcggca aaaacgccgc ccccgacacg ccgactttgg
    12781 tttatgcata cggcggtttt ggcattcccg agttgccgca ttatttgggc agcgtcggca
    12841 aatattggct ggaagagggc aatgcctttg tgttggcaaa catccgcggc ggcggcgagt
    12901 tcggcccgcg ctggcatcag gcggcgcagg gaatcagcaa acacaaaagc gtcgacgatt
    12961 tgcttgccgt cgtgcgcgat ttgtccgaac gcggtatcag ttcgccccaa cacatcggct
    13021 tgcagggcgg cagcaacggc ggcctgatta ccgccgccgc cttcgtgcgc gaaccgcaaa
    13081 gcatcggcgc gctggtgtgc gaagtgccgc tgaccgatat gatccgctat ccgctgctct
    13141 ccgccggcgc aagctggacg gacgaatacg gcaacccgca gaaatacgaa gtctgcaaat
    13201 gccggttggg cgaattgtcg ccgtatcaca atctttcaga cggcatcgat tatccgccag
    13261 cgctcattac caccagcctc agcgacgacc gcgtccatcc tgcccacgcg ctcaagttct
    13321 acgccaaact gcgcgaaacc tccgcgcaat cttggctcta ctcacctgac ggcggcggcc
    13381 ataccggcaa cggcacccaa cgcgaatccg ccgacgaact cgcctgcgtg ttgctgtttt
    13441 tgaaagagtt tttgggctaa gggcggggag cggcactgcc gccgcgaatg aaaaaaggtc
    13501 gtctgaaact gctttttcag acgacctttt ttaatggt
//
LOCUS       DAWFAN010000041        13383 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWFAN010000041 DAWFAN010000000
VERSION     DAWFAN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.041"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(100..528)
                     /locus_tag="WGO65_000840"
     CDS             complement(100..528)
                     /locus_tag="WGO65_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ0327156.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLGYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(593..2341)
                     /locus_tag="WGO65_000841"
     CDS             complement(593..2341)
                     /locus_tag="WGO65_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ0327157.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSNIKKICETELTVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2668..4101
                     /gene="ccoN"
                     /locus_tag="WGO65_000842"
     CDS             2668..4101
                     /gene="ccoN"
                     /locus_tag="WGO65_000842"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ0327158.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4128..4739
                     /gene="ccoO"
                     /locus_tag="WGO65_000843"
     CDS             4128..4739
                     /gene="ccoO"
                     /locus_tag="WGO65_000843"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ0327159.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4744..4914
                     /locus_tag="WGO65_000844"
     CDS             4744..4914
                     /locus_tag="WGO65_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ0327160.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4938..6035
                     /gene="ccoP"
                     /locus_tag="WGO65_000845"
     CDS             4938..6035
                     /gene="ccoP"
                     /locus_tag="WGO65_000845"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ0327161.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6148..6273
                     /locus_tag="WGO65_000846"
     CDS             6148..6273
                     /locus_tag="WGO65_000846"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0327162.1"
                     /translation="MHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            6475..7884
                     /locus_tag="WGO65_000847"
     CDS             6475..7884
                     /locus_tag="WGO65_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0327163.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7986..11195
                     /gene="recC"
                     /locus_tag="WGO65_000848"
     CDS             7986..11195
                     /gene="recC"
                     /locus_tag="WGO65_000848"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ0327164.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPHIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGAGAA"
     gene            complement(11265..12833)
                     /gene="mtrF"
                     /locus_tag="WGO65_000849"
     CDS             complement(11265..12833)
                     /gene="mtrF"
                     /locus_tag="WGO65_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ0327165.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFELSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13167..13379
                     /locus_tag="WGO65_000850"
     CDS             13167..13379
                     /locus_tag="WGO65_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327166.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIARDVSLTTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3351 a         3219 c         3553 g         3260 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgttttc ggcttgtaat cagacgcggg cgttgtccag
      121 ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga tgatttcgtc
      181 aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc gcgtaccggt
      241 ccggcaatag ccaaggacgg gatattcggc ttgtccgatg agttggcgga aggtttcgac
      301 atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag taacgcctgc
      361 ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt cgcggtcggg
      421 gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat cggctttggt
      481 cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt tgtttccttt
      541 actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa gtttaaccga
      601 acaaccagtt gtccaacagg tttcggtctg tgatatgtag gatggcagta tccgctgccg
      661 ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag tggatattga
      721 tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag gcataaccgt
      781 ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg ccgccgttga
      841 agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca aaatcgcctg
      901 ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg ggaagcggca
      961 ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa tcttcggtac
     1021 tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc tgacagcgga
     1081 tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga tagagtttgt
     1141 ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc agatcaaggc
     1201 acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg ctgttttcat
     1261 ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc tgcctcaaac
     1321 ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa taagattcgg
     1381 gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta ataccttcga
     1441 atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg aacgcggcag
     1501 gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt ccgagcaatg
     1561 tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg cggtcggcga
     1621 gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg acgtggagca
     1681 ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgtcagttct gtttcgcaga
     1741 tttttttgat attcgaaacc agcctgttgc ggtcgaaatc gggatatatg ccgcttaagg
     1801 caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag cccatctcga
     1861 caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc cctgtttccg
     1921 gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag cggtgcggca
     1981 gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa ccgcgttctg
     2041 tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag cggatttgcc
     2101 actcgccgcg tacggcggca gcgtgccagc ggttttttgc aatttgttcg agcggcatgg
     2161 acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc cgaatcagat
     2221 agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca tcttgtgtga
     2281 atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta taatgaatca
     2341 taactatcaa atatatcgtt aaacgggata gattaaatac ctttaaaatg cagcttaacc
     2401 tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg cggtcgggtt
     2461 gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc aaagggctcg
     2521 tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa atttagaaaa
     2581 aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga taaaaatctt
     2641 tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta caaagtggtg
     2701 cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt cggcgttatc
     2761 gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc ttggttccac
     2821 ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg cggttgcggc
     2881 ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct tttcggcggt
     2941 tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc cgtcgtcagc
     3001 ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc gatcgatatt
     3061 ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat tgccaaacgt
     3121 aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt ggcggtcgca
     3181 cttttacaca tcgtcaacaa tatcagcatc ccggcagggt tgatgaagtc ataccccgtc
     3241 tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataacgc cgtgggcttc
     3301 ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca agcagcccgc
     3361 cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt tacctatatg
     3421 tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca atctttgggt
     3481 atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa cggcatcatg
     3541 accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt tctgattgta
     3601 tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat taaaacggtc
     3661 aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg tgcgttgggc
     3721 tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct gttcggcaaa
     3781 gaacagatgc acagcaccaa gctggtagaa gcgcattttt ggattgcgac catcggcgtg
     3841 gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat gtggagttct
     3901 ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg caccatgcct
     3961 tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg cattatggcg
     4021 tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat ccctgcggtt
     4081 tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg aaattacaac
     4141 aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta gtcagtgtcg
     4201 gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag ccggcaccgg
     4261 gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc cgtgagggct
     4321 gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag cgttacggtc
     4381 attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt tccaaacgta
     4441 ccggtcctga tttggcacgt gtgggcggcc gctattccga cgaatggcac cgtatccacc
     4501 tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca tggcttgcac
     4561 gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt aaagtaggta
     4621 ctccttacag tgatgaggaa attgcgaaag cacctgaggc tttggcaaac aaatccgagc
     4681 tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac gtaaggtaac
     4741 atcatggata ttaacggtat tcgtgctctc ttcacggtat ggatctttat ctgtttcctg
     4801 ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa tgccgcaaac
     4861 agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg ttaatattgt
     4921 gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc tggaatatat
     4981 atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg ctgtctcaaa
     5041 atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat gagtgggacg
     5101 gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt gttttgacgt
     5161 ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac aaaggtctgc
     5221 tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat gagcaatacg
     5281 gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa gaccctcaag
     5341 ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac ggctctgatg
     5401 ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg ggcggtgatc
     5461 ccgataaaat tcacgagacc atcgaaaaag gccgtgttgc gactatgcct gcctggggcc
     5521 ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc ctttcaaaac
     5581 ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc agcggtccgc
     5641 ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt ttgggtccga
     5701 acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc gaaaccatta
     5761 ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa gacaaactgc
     5821 atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct ccggtgaaaa
     5881 aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct gctcctgcag
     5941 aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa gccgaggaaa
     6001 aagccgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt tgtgccgcct
     6061 gccatggcaa tgcggttccg ggtattcccc atgtaggcat caaagccgat tgggccgacc
     6121 gcatcaaaaa aggcaaggac acgttcgttg cacaaacacg cgatcgaagg tttcaatacg
     6181 atgcccgcaa aaggcggtcg cggcgatttg agcgatgatg aagtgaaagc tgcggttgac
     6241 tatatggtca accagtcagg cggcaaattc taacttgact gagtttccga tattatcggt
     6301 tatgaaaatc cagattcagt attgaatctg gattttttgt tttaacgggc atccggtttt
     6361 aaacgcagac tgcctatttg ttgtaatgaa ttttgacttt agtcataaag aattagtctt
     6421 tattgtttat aatcgcgcat catcacgata gccctatttt caaaaggacg attaatggat
     6481 acacaaatca aaactgaagc cgacaatcag agcaaccggc gttatctgac cgtttggcgg
     6541 tggcattttt atgccggtct gctggttatg ccttttctta ccctgcttgc cgttacgggt
     6601 ttgggtatgc tgctgtttgc caatattacc ggtaaggagg gcgagcggat tcatgttgtg
     6661 ccgcaggcaa cggtacaacc tctgtctgtt caggcggaag cggcacgcag tgccgttaat
     6721 ccggaaactt cgtccgtcgt tcagtatatt gcgccgcgtg ccgatgatat ggttgccgtg
     6781 ttccgtgtca acaatgaggg caaggcaacg atggtcgcgg tcgatcctta tacggcaaaa
     6841 gtggtcagta ccatgccgcg caatcagggt tggtattacg cgatggatga aatccacagc
     6901 gatatgatgc tcggttcgac aggtgattat cttttggaaa cggctgcatc gctgacgatt
     6961 atcatgataa tcagcggttt gtacctttgg tgggtgaaac ggcgcggcat caaggcgatg
     7021 ctgctgccgc caaaaggcag ggcgcgttct tggtggcgga atctgcacgg cgcgtttgga
     7081 acttgggtgt cgttgatttt actgttgttc tgcctgtcgg gtattgcttg ggcaggtatt
     7141 tggggcggca agttcgtgca ggcttggagt cagttcccgg ccggcaaatg gggtgtcgaa
     7201 ccgaaccctg tttcagtcgt gccgacccac ggcgaggtat tgaatgacgg caaggttaag
     7261 gaagtgccgt gggttttgga gcttacgcct atgcctgttt cagggacgac tgtgggcaaa
     7321 gacggtatta accctgacga gccgatgaca ttggaaaccg tcgaccgttt tgcgcgggaa
     7381 atcggtttca aagggcgtta tcagttgaat ttgcccaaag gcgaggacgg agtgtggact
     7441 ttgtcccaag actctatgag ttacgatatg attagtccgt ttgccgaccg cacggtgcat
     7501 atcgaccagt acagcggcaa aatccttgcc gacatccgct ttgacgatta caacccgttc
     7561 ggcaaattta tggcggcaag cattgcgctg catatgggta ctttgggctg gtggagcgtg
     7621 ttggcgaacg ttttgttctg ccttgccgtc atttttatcg gtatcagcgg ctgcgtgatg
     7681 tggtggaaac gccgtccgac cggagcggtg ggcatcgttc cgccggcgca gaaagtcaag
     7741 ctgccggttt ggtggatgat ggcattgccg ctattggcaa tcgcactgct cttcccgacc
     7801 gcactgcttg ccattgccgt gatttggctg ttggatacct tgctgctgtc gcggattcct
     7861 gttttgagga gatggtttaa atgacccgat gccgtctgaa gcgttcagac ggcattttgt
     7921 ttgaaggcgg gcgggggaaa ggctatataa tcccgaatac ttgaccacag cttctgtttg
     7981 aaatcatgtt ttatctgtat caatccaacc gtcttgaaac gctggcggca ctgtttgccc
     8041 gcattcaaaa agtcaaaccg ctgaaatcgg ttttacagcc cgaacagatt attgtgcaga
     8101 gtcaggggat gcgccgctac ctcaatacct gcctcgcccg cgatttgggc gtggcggcga
     8161 atttgtcgtt cagcctgccc gccggtctga cgtggaagct gatgaaaaaa ctgattcccg
     8221 gtattccgga actcagcccg tttgcgcctg aagtgatgcg ctggcggctg ttggatttgt
     8281 tccgcagcga ggcatttcgg aatacggcag aatttgaaga tgtgaggaat gtgctgcaag
     8341 actatctagg cagcggcgaa tcggcagatt atcagcttgc gggacagctt gcggacatat
     8401 tcgaccaata ccttgtctat cgtccgcagt ggatagacgc gtggcagcag ggcaggatac
     8461 tcagtttggg cgacgatgaa atctggcagt ccaaactgtg gcgttatctt gatgacggca
     8521 ggcagagcgc gccgcaccgt gtcgcgttgt gggaaaagct gcttgccgcg ttggacaagg
     8581 ataagctgcc cgagcgttat ttcgtgttcg gcatttccac gatggcgccg atgtatcttc
     8641 agcttttgca caagctgtcc gaacattgcg atgtgttcgt gttcgcactc aatccgagcg
     8701 ggatgtattg gggcaacgtc atcgaagcgg cgcaaatcct caaaggcggc ggcgatcccg
     8761 atttaactca ggcagggcat ccgctgctcg cctcattggg caagcagggg cgcgactttt
     8821 tcgacttttt gaacgaaatg gaaatagaag gagaaacgcc ggtatttgaa gaaggcgggc
     8881 gcgatacgct tttgcacgcc ctgcaaaccg atattcaaaa cctgaaaatg ccgtctgaaa
     8941 atgtgggaag cgtcaacacg ggcgacggct cgatacgcat cgtctccgca cacagcccct
     9001 tgcgcgaatt gcagatactc aaagacaagc tgttgaaaat tctgcatgaa catcccgatt
     9061 ggcagccgca tgatatcgcc gtattaaccc cgcacatcga atcctatacg ccttttatcg
     9121 aagccgtgtt cggacaggcg cagcccggtg cgcaggcatt gccgtattcc gtttcagacg
     9181 tgaaaatcag ccgccgacaa ccgctgtttc atgctttgtc atgcctgttc gacttgttgg
     9241 aaagccgatt tgaagtcgat aaagtgcttg tgcttttaga aaccgcaccc gtgttgcgcc
     9301 gtttcggact gactgaggac gatttgccgc ttttgcacga tatggttgcc gatttgaacg
     9361 ttcactgggg tttggacgga gaaatgcgcg gcggcacgga tcagctgttt acctggaagc
     9421 aggcggtaga acgcatgata ttgggctgga tgctgcccga aggcggcaac ccgatgtggc
     9481 aggatgtcag cgcgtggtat gccgacgtga accaaaccgc cctgttcgga cgttttgccg
     9541 ccctcctcga aaccctgtcg gatattgtac ggatatggcg gcagcccgca acgatcggcg
     9601 aatgggtggc gcgttgccgg gatttgcttg aaacattgtt ccaagctgaa gccgatgacc
     9661 aaaagtcagt ccaaaacctt gaaaacgaat gggtcaaatg gcaggcggaa acagaattgg
     9721 cgcaattttc cggacagttg ccgcaacaaa ccgtcatccg ccatatccga cgtttcctcg
     9781 acagcgaaag cgaggcaggc tttttacgcg gcggcatcac cttttgcagt atggtgccga
     9841 tgcggagcct gccgttcaaa gtcatctgcc tgttgggttt gaacgacgga gattttcccc
     9901 gcaataccaa agccgccgta ttcgacctga ttgccaaaca tcccgccaaa ggcgaccgcg
     9961 cccgccgcga tgacgaccgc tacctcttcc tcgaagccct catcagcgcg cgcgaaatcc
    10021 tctacctgtc ctacatcggg cgcgacatcc gcaaagacga agagcttgct ccgtcttcgc
    10081 tgttgggcga actcatcgac acagttgccg ctatggcggg catcggtagc cgccaacttg
    10141 cacaaaactg gatagaacag catccgctgc aagccttctc gcgccgatat tttcaagaag
    10201 gcggacgttc agacggcata ttcggcacgc gtaccgacta cgccactgcg ctcggacaaa
    10261 cgcccgaacc gccgcaaccc tttttcgacc aacccgtaga aaacgccgaa cctgttgccg
    10321 aaatcggaca ggacgaattt atccgtttct ggcgcaaccc cgtcaaagta tggcttcagc
    10381 agcagcttgc gtggagcgaa ccccatatcg gcgaagcctg ggagcctgcc gaacccttcg
    10441 agccgcaaca cgccgatcaa atcgccgaaa cctacatcga agcacggcgc gaaggacggg
    10501 attttgccca aaccgccgcc cgcatcgggg cggaaagcct cctgccgtcg ggagagttgg
    10561 gcagactttg gcagcagaac ttccaaactg ctgccaaaca aatcgacacg gcggttttaa
    10621 acagccccaa actgccaccg ctttcatatg ccataccgtc ggacgggcaa atcctgaaag
    10681 gcagcttggg caatctgtac cgctgcggac aagtgtttta cgcctacggc aaacccaacg
    10741 cgccgcaacg tatcgctttt ctgctggaac acctgatatt ttgcgccgtt atgccgtctg
    10801 aagccgaaac gcggcaaacc tttatcgtcc aatccggaga aaccgaaata ttggcggaaa
    10861 tcgcgcaaga cagggcattg cagctattgt cggaatggat ggcgtttttc aatatcgggc
    10921 aaaaccgccc gctgccgttt tttgccaaga cctcgcttgc cgctgccgaa gcgtttgccc
    10981 aaaaacaaga ttgggaagcc gccctgaaaa aagcccaaac cgcctattac ggcaacaaag
    11041 tcagcaaagg gcagaaagac tataccgaag tcgccctcgt gttcggcaac gcaagccaaa
    11101 acccactcga acaacccctg tttgaaaacc tcgcccgcct gcttgccgac acgcttgccg
    11161 cagcggaaaa aagggaaggg gccggagcag cctgacggga tggaaaggca aatgccgtct
    11221 gaaaacagca aaaaggtttt cagacggcat tttgtttatc gtgtttaagg tgcgggatag
    11281 aatgtgggcg cgccgggacc gacgggcagg cccaaaacaa atacccaaat gcagaataag
    11341 gcaatccacg caatcaagaa gaaagcggaa tacggcaaca tcatagaaat cagcgtaccc
    11401 acgcccgcat cttttttgta tttgatcacc gtcgccataa tcagcccgaa ataactcatc
    11461 atcggcgtaa taatattggt aacggaatca ccgatgcggt aagcggcttg aatgacttcg
    11521 ggcgcgtagc cggccaacat cagcataggg acgaaaatcg gcgcagttac cgcccattgc
    11581 gcggaggcgg agcctatcat cagattgata aaagcacaaa ttaaaataaa accgataaac
    11641 aacacgctgc cgcccaagcc gacttctttt aagaacgtcg cccctttaac ggcaatatat
    11701 tgcccaatat tcgtccaatt aaaaaatgcg acaaactgtg cggcaaaaaa gatgatgacc
    11761 aaataaagcc ccagagtact catcgattcg gccatcgcat taacgacttc ctgttcgccg
    11821 cgcaaacttc gggttacccg gccataaaca atgcccggca gtgcaaacaa caagaaaata
    11881 aaaacaacaa ttgattttaa aaacggcgaa ccggaaacca atcctgtttc aggatgacgc
    11941 aaaataccgt cggcagggac gatgctccaa gccaataggg cggataaggc aacaaacacc
    12001 acgccagccc aaattaatcc tttatattcc aaaggcgtga tttcattgga atgtcgaatg
    12061 tctttttctt cttgtgacaa atctgattga taagggccca attgcggttc gacgattttt
    12121 tcagtaacaa aataaccaat caaagcaatc acaaacgtac tggctaccat aaaaaaccag
    12181 ttggcttcag ggcctacgac gtagtcggga tggatgattt gcgccgcctg ttgggtgatg
    12241 cctgccaaga gcggatcgat tgtgcctaag aacagattgg ccgaataacc gcccgaaacg
    12301 ccggcgaaag ccgcagccag accggcaagc ggatggcggc cgagggaatg aaagatgatg
    12361 gcggacaaag ggattaggac gacatagccc aattcagaag cggtattaga taaaatccct
    12421 gtaaaaacaa ccataaaagt agtgagtttg cgtggagatt ttgtgagcaa taagcgcatt
    12481 aatgcggaaa tcaagcccga tttttccgca atccccacgc ccaataaaga aaccaacacc
    12541 gttcccaacg gcgcgaaacc ggtgaaattt ttaacggtat gcgtcaggat tttgatcaaa
    12601 ccgtcagcat cgagcaggct gacaacgtga atcaaaccgt catcggcacg tcctttcgca
    12661 ccaacagggc gcggatcggg gacggatagt tcgaaatacg caccggcggc agaggcaatc
    12721 agcaataaca caatgaaaat aataaaaagc gtaaccgggt gcggcaacat attgcccagc
    12781 cattcgactg tgcgtaaaaa tcgtccgtcc cgttgcgtat cggtttgact catctgcttc
    12841 tccttaaaaa aaatatattg agtgcattaa atttttgaat gtaacatgaa gtgttttgta
    12901 agcaaactaa aataatcgta tatgtatata accgtaatag attaaaacag aaaatgccca
    12961 catcgttatt ctcataaagg cggtaatccg aaaacttgac gttaaagcct tatcaggaat
    13021 gactggaact caaaaaaacg gattcccgcc tgcgcgggaa tgacgggatt ttcggttttt
    13081 gtttgcaaga atgccggaga agaatgcagt ttacattttg aatggtattg ttattaattg
    13141 taaataaata ttggaaggta aatgatatga aaaatgaaat tcaaaaaatc atggacaaat
    13201 ataatccatg gcatgaagat gattttgagt cgtatgaaga cattgccaga gatgtatcgc
    13261 tgacgacaga taaaacgttc attgaacatt atttgttaga agtttattca gaagaaaacg
    13321 gacattttga ccaagagaat gtccatgcaa tgatagaaga aattaaaaat gcaatttagt
    13381 gaa
//
LOCUS       DAWFAN010000042        13057 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWFAN010000042 DAWFAN010000000
VERSION     DAWFAN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.042"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            142..309
                     /locus_tag="WGO65_000851"
     CDS             142..309
                     /locus_tag="WGO65_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327167.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYTQNIDFSQW
                     VKSIIKEKIKK"
     gene            582..767
                     /locus_tag="WGO65_000852"
     CDS             582..767
                     /locus_tag="WGO65_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327168.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            968..1168
                     /locus_tag="WGO65_000853"
     CDS             968..1168
                     /locus_tag="WGO65_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327169.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            1300..1908
                     /locus_tag="WGO65_000854"
     CDS             1300..1908
                     /locus_tag="WGO65_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HEZ0327170.1"
                     /translation="MKDNFTMNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSK
                     WIQRRIEEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERND
                     KGRQARRYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIG
                     KLLDMNPGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            1912..2127
                     /locus_tag="WGO65_000855"
     CDS             1912..2127
                     /locus_tag="WGO65_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327171.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(2288..2806)
                     /locus_tag="WGO65_000856"
     CDS             complement(2288..2806)
                     /locus_tag="WGO65_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ0327172.1"
                     /translation="MAKTRIKQPAIEAAQDKAEVTAFIRKIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(2831..3319)
                     /locus_tag="WGO65_000857"
     CDS             complement(2831..3319)
                     /locus_tag="WGO65_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327173.1"
                     /translation="MKELPTQLHNAMIDGLTMLLTLRLSGSPAADTVAATAQTWSRVL
                     AHGRAWDEARDVPRFQTAFMVLANEMSRWPSPKDFLDKIPPPPEPLKLEHHYHPTAEE
                     KAKGKSALNRIQGVIKEVLKGKSLIPPPAETATGQILRNRAKVEALAKREREQGLSKP
                     KC"
     gene            complement(3316..3801)
                     /locus_tag="WGO65_000858"
     CDS             complement(3316..3801)
                     /locus_tag="WGO65_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327174.1"
                     /translation="MNVKCPNCGAVHSLDSLINDAEASAVLKAVLEMDVELGKAAIRY
                     IGLFRPAKSRLSWARTAKLLNELLPMIKAQEATRDGVCFHAPAEAWIHGFNETVNARD
                     QGRLKTPLKSHGYLYEILAGRVGQPSAGNQTNQPNRRAALPANPSQTLTAAASLQGLK
                     K"
     gene            complement(3798..3998)
                     /locus_tag="WGO65_000859"
     CDS             complement(3798..3998)
                     /locus_tag="WGO65_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327175.1"
                     /translation="MMVTLIYFGVVFVLGLGMTLVCVANFLKGRCSRKSEILGALVIV
                     FGSVATWLATWLSMMVLSGVIK"
     gene            complement(3995..4168)
                     /locus_tag="WGO65_000860"
     CDS             complement(3995..4168)
                     /locus_tag="WGO65_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327176.1"
                     /translation="MANIDLTQWGTKLQVIPQRFEHEPAIYEVECGNDSVFFTEEHAY
                     QLYYALEKVLEDL"
     gene            complement(4168..4335)
                     /locus_tag="WGO65_000861"
     CDS             complement(4168..4335)
                     /locus_tag="WGO65_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327177.1"
                     /translation="MNEKDLIEWLEDRGELMIMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            complement(4328..4501)
                     /locus_tag="WGO65_000862"
     CDS             complement(4328..4501)
                     /locus_tag="WGO65_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327178.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATKFMVKRNREQEVAEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            complement(4607..4846)
                     /locus_tag="WGO65_000863"
     CDS             complement(4607..4846)
                     /locus_tag="WGO65_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327179.1"
                     /translation="MSHLFCERKTKWIGLAFWLLFWAVLVGTMLHSCSKPKVSAAKLE
                     MSRRERMADLEVKIWEEQYESMSVEEKMKGIVYER"
     gene            complement(4874..5095)
                     /locus_tag="WGO65_000864"
                     /pseudo
     CDS             complement(4874..5095)
                     /locus_tag="WGO65_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520480.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
     gene            complement(5111..6025)
                     /locus_tag="WGO65_000865"
     CDS             complement(5111..6025)
                     /locus_tag="WGO65_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEZ0327180.1"
                     /translation="MKQINQALQQKLAEFKAKSGMNQTQLARGIGTSPASISMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKAKREELVLGFVSTKTTRRIAEAMRDAHEGGEIV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSLPLNLGESLPDDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            6096..6173
                     /locus_tag="WGO65_000866"
                     /pseudo
     CDS             6096..6173
                     /locus_tag="WGO65_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(6240..8213)
                     /locus_tag="WGO65_000867"
     CDS             complement(6240..8213)
                     /locus_tag="WGO65_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0327181.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DARCAVVAAVLGIKYQYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRTAKVG
                     ERTLDGWISAYLKAEDATERLVALAPKTTKTVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPGWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPDMLSA
                     EELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASAVNVFDMD
                     GKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQAAVW
                     DELGHLGGNVIEAEYAVLPKTGTDDFVLFEADR"
     gene            complement(8215..8466)
                     /locus_tag="WGO65_000868"
     CDS             complement(8215..8466)
                     /locus_tag="WGO65_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0327182.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            8514..9413
                     /locus_tag="WGO65_000869"
     CDS             8514..9413
                     /locus_tag="WGO65_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ0327183.1"
                     /translation="MPALTVTWMIYTKYLVYQIYLVIFCDVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            9575..9787
                     /locus_tag="WGO65_000870"
     CDS             9575..9787
                     /locus_tag="WGO65_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0327184.1"
                     /translation="MNHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAP
                     FEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            10009..10587
                     /locus_tag="WGO65_000871"
     CDS             10009..10587
                     /locus_tag="WGO65_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ0327185.1"
                     /translation="MFRHNGEVLLDGINIKLKSGDSLLIRGPSGCGKTSLLRALAGLW
                     PFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELAEAMNTCRLGYL
                     IDKLDKTDDWQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALDEPTEALLYRALKQ
                     KLPDSIIISIGHRSTLNAFHNMHLDVGNVACG"
     gene            10692..11360
                     /gene="hda"
                     /locus_tag="WGO65_000872"
     CDS             10692..11360
                     /gene="hda"
                     /locus_tag="WGO65_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ0327186.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            11357..12025
                     /locus_tag="WGO65_000873"
     CDS             11357..12025
                     /locus_tag="WGO65_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0327187.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            12381..12488
                     /locus_tag="WGO65_000874"
                     /pseudo
     CDS             12381..12488
                     /locus_tag="WGO65_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         3442 a         3500 c         2716 g         3399 t
ORIGIN      
        1 ctattcaaat tctcatcttg gtcgcagttt tcatgttggc aagtgctaat gcctactaaa
       61 gactgaccag ctgggggcgg tcgccgctgt ccccagtccc aatcttacaa aatacggttt
      121 aaaaaatcaa ggagtttgcc tatggttgat gaaaaactgg cagaaaaccg aaagcgttac
      181 gagcagaaac gggtgattaa gaaagtctcg ttcaatgccg aaacggaaaa agaacttttg
      241 gaatataccc aaaatatcga tttttcccaa tgggttaaat caatcatcaa agaaaaaatc
      301 aaaaaatagc ttgactaaat tctagaaata ctagataata cgcctatctg ataaagcaaa
      361 gccccgaagc ggcaacttcg ggggcttatc ggatagaaag gaggcttgtg gaaatgacaa
      421 aactggcgaa agttgttttt ttcatcattc tgcttttaag ctgtagtccg gcttactaag
      481 caagttgatt aaacacaagg ggtggcagaa caccgcgccc ctttcgccaa cctttctgca
      541 agccatttta ctacaacgat tttaaaaatc aaggattgcc tatgcctgat gaaaaaacgc
      601 taaaagcaaa acaagctgaa tatcagcgaa agtatgacca aaagcggctg atgaaaacag
      661 tttctttcca tttggaaaga gagaaagagt tggtggattt tatgcaaaaa gaaattcccg
      721 acttttcaaa ctgggttaaa tcagtcatga aggaaaaaat gaaataagaa aattccgccg
      781 atgggaaaac atcggcggaa caggtggaaa gcagagcagc aactctcttt atgtagggca
      841 tgaaaaaagg ccgtctgaaa caggttttaa accccatttc agacggcttt ttgttgtaat
      901 acatcttgat taaaatggtt tttagtatta ccataatcgt gttttaaaac ctatttaaag
      961 gaagattatg gactcgatta aaaaggctca agaaaaatac gcactcaagc gagtagtgaa
     1021 aacagtatcg ttcaattccg aaaaagaact tgatttaatt aaattttctg aaaatttaga
     1081 tttttcaaac tgggtaaaat cgaaaattag aggagatatg gaatcggaaa aattcaaaaa
     1141 aaaagcaaaa aagaatattg acaaatagaa ttaccataat ttaaaatttc catatcttgt
     1201 gcaacacaag atatggaaat ccccgataag tgacagctta tcggggatag tggaaaagca
     1261 acgttagagc gttcttttcc ttgtcatctc aaccttgaaa tgaaagataa ttttaccatg
     1321 aatacccaac tcatcccaac tgtttcaggt caattggaca atcaaacaca agcactggtt
     1381 gatgctcatg acctgcataa gttcttaggt gtggaaacac cattttctaa atggattcag
     1441 cgtcgcattg aagaatacgg atttacacaa gcccttgatt ttatcggcgt ggacaaaatt
     1501 gtccgcaccg aagcaggttt ctttggacag cgtgataaaa ctgttcaggg gtattattta
     1561 tccctcgaca tggcaaaaga gctgtgcatg gtggagcgca acgacaaagg acgccaagcc
     1621 cgccgatatt ttatcgaaat ggagaaacag gcaaaagccc tgcctgatgc cgtgctatac
     1681 cgcatcgatg cattggaaga cgcctatttc caagccgcgc ccgaaatgct cgcgctgctg
     1741 cgctaccgca gtatgggctt gaacctgacc gaaataggca aactcttgga catgaacccc
     1801 ggcgcggttt cctaccgtct caaaaaactc aacgatttgg gtttcttgga atatgtcccc
     1861 aagcccaaaa tggtggtgca acaacagtct ttgggattgg agggttaagc catgaatacc
     1921 acctataccc tgactttcga ccaagacagc ctcaaagccc tgaatctgct ggtgttcaca
     1981 ctcaaccatc tgaaagtttt ggagatggac atggaaggaa tcgaagacgg tttgtctgcc
     2041 gtgattgaaa ccgcctccga gaaagccgac aagctgtccg ccgctttcta caacgccgtg
     2101 tacgagcagg aagagaaagc agcctgaatc tagggcatga aaaaaggccg tctgaaacag
     2161 gttttaaacc ccgtttcaga cgacctttaa atcatgggtt aaagcctatt tgaaccggct
     2221 aggcaagctc ctgctccgta ggctcaatca cgtttcaaca cactgaattg aaatcaggct
     2281 gttgatttta tagaatttgt tcgctcggct caatcacaaa atcctccacg ccgctcacaa
     2341 tcttaatccc cggcacttgg ccgtctgaaa aacgctcttt ttgattcagg atggcgtctt
     2401 tgtcgatttc ctttttagtg cggacaaact cggcaaaggc ggatttttcc gagagccacg
     2461 ccaagacggc ggccacgccc gttaccttga cggatggcgg acggatgcgc catttaatca
     2521 ggccggtggt aaagtccacg gttttggttt taccgttttc cgtcagctcg tccttatgtg
     2581 cctcgcagta tgcggccaca cgttcggtca ggctcatgat ttcggcacac atcggcgcgg
     2641 ctttggcggc atattcttct tcgatggccg cttttttgtc tccggcttcg gtttccaggc
     2701 gtttgacttc gcgttgcaaa tcgccgattt tgcggataaa cgcagtaact tccgctttgt
     2761 cttgtgccgc ttcgatagcg ggttgtttga ttcgggtttt agccattttc tctttccttt
     2821 caggttgggt ttaacatttc ggcttgctca agccttgttc gcgttcgcgc ttggcaagtg
     2881 cctcaacttt cgcgcggttt ctcaaaatct gcccggtggc ggtttcagcc ggaggcggta
     2941 tcagcgactt gccttttaat acctctttaa ttacgccctg tatgcggttt aaagccgatt
     3001 tccccttcgc tttttcctct gccgtagggt ggtagtggtg ttccagcttc aacggctccg
     3061 gtggcggcgg aatcttgtct aaaaaatctt tcggactcgg ccagcggctc atttcattgg
     3121 ccaataccat aaaggccgtc tgaaagcgcg gtacatctcg cgcttcgtcc cacgcccggc
     3181 cgtgcgccaa tacacggctc catgtttgcg cagtggcggc cacagtgtcg gcagccggcg
     3241 aaccgctcag acgcagggtc aaaagcatgg tcaggccgtc aatcatggcg ttatgcagtt
     3301 gggtaggcag ttctttcatt ttttcagtcc ttgcagcgag gcggctgcgg tcagggtttg
     3361 gctgggattg gccggcaggg cggcgcggcg gttgggttgg tttgtctgat tccctgcgct
     3421 tggctggccg acccggcctg caaggatttc atacagatag ccgtgcgact tcagcggcgt
     3481 tttcagacgg ccttggtcgc gtgcgttaac ggtttcgtta aatccgtgaa tccaagcctc
     3541 ggcaggggca tggaaacaaa ccccgtcgcg tgtcgcctcc tgcgccttaa tcatcggcag
     3601 caactcattc agcagtttcg cggtacgcgc ccaagagagc cgggacttgg cagggcggaa
     3661 taaaccgata taccgtatcg ccgccttgcc caattccaca tccatctcca acacagcctt
     3721 taataccgcc gaagcctccg catcattgat taagctgtcc aggctatgca ccgcaccaca
     3781 gttcgggcat ttcacgttca tttgattact ccggacaaca ccatcatcga caaccatgtg
     3841 gccaaccatg tggccaccga accgaaaaca atcaccagtg cgcccaatat ctccgatttc
     3901 cgcgaacagc gacccttgag gaagttcgcc acacaaacca aagtcatgcc aagacccaac
     3961 acaaacacca caccgaaata aatcaacgta accatcataa atcctccaaa accttttcta
     4021 gtgcgtaata aagctggtaa gcatgttctt ctgtgaaaaa tacggagtcg ttaccacatt
     4081 caacttcata aatcgcaggt tcatgctcaa atctttgcgg gattacctga agcttcgttc
     4141 cccattgagt taaatcgata tttgccatca cacttcctcc cataaagtta tcgcctgagc
     4201 caaagtttcc gcctcggcag ttttccacat cccatccggc gcacgcgctg caatcacaaa
     4261 gccttcgccg tcctttttca taatcatcag ctcgccacgg tcttcgagcc attcgatcaa
     4321 atctttttca ttcatctttg tttccttccg ttttcagacg acctttgccg tccttatctt
     4381 caaactgcgc ctgatattcg gcgacttcct gctcgcggtt tcgcttcacc ataaacttcg
     4441 tcgcacgcct gcggtgttgt ccccaagcct gccaatccgt attacgtcgt ccgaaactca
     4501 tttcacacat cctttcacaa tcgccttatc gccatatttc gcgcggattt cctttaccgc
     4561 ccgatccaaa gcctcttttt tcgccgcagg gttcaataaa attggcttat cgctcataaa
     4621 caatcccttt cattttttcc tcgacgctca ttgactcgta ttgctcttcc catatcttga
     4681 cctccaaatc cgccatccgc tcgcggcgcg acatttccaa cttcgccgcc gacaccttgg
     4741 gcttagagca gctgtgcagc atcgttccca ccaaaaccgc ccaaaacaac aaccaaaaag
     4801 ccaaaccgat ccacttggtt tttcgttcgc aaaacaaatg agacattttc atatttcctt
     4861 ataaatcaac taattaatat tttcttaagg caaaaaaatt attgcgtacc caatccgccc
     4921 aaccgacgcg cccccttcgc tccctctacc cgtgggagag ggtcggggag agggcaactc
     4981 cttctttgcg cttgccgcgt acatccgccc gccaaatctt ccgtcgttta tgggtttcat
     5041 aatcgtgcca agtgtcctca tagactccgg cgtgtaccgc atattcgtgt ctcatttcag
     5101 acggcctttc ttatcggata atcagggagc tgtatttctt aacgataccg gcctgcatct
     5161 tgataccgtt cttgttcgcc gtgcgtaccg cgccgcgcat caatttgctc atccggcgcg
     5221 tattgccgtt gctatgttta accagttcca agagcgtttc ttcgtccgca tcaggcaaag
     5281 ccgctttcgc aatctcaaag agttcgtcat ccggcaaaga ttcgcccaaa ttcagcggaa
     5341 gcgacacgcg gctgtaaagc tgtaccagct cgccatgctt accgcgcaaa ttcgcaacca
     5401 gtcggggcat accgctcaac accaaaccgc aacccgtttc atcatgcagt cggcggataa
     5461 tctcaagggc gcgcaaaggc aggttttccg cttcatcgac cacaatcaga cggcccgaat
     5521 cacgcaatct gtccgatacc gattcaaaca aatcattcag gctgcccatc gccgatacct
     5581 tcgccgccgt cgccaacttg cgcatcaaga ccaaagccgt aaagctcgga ttagcctcaa
     5641 tcaagatggc cgctgggttt ttctcgcagt agtttttgac cgcctgagtc ttgcccaaac
     5701 cggcctgacc gtagatcacc actatttcgc cgccttcgtg cgcatcgcgc atcgcttccg
     5761 caatacggcg tgtcgtctta gtcgatacaa aacccaacac cagctcttcg cgtttcgctt
     5821 tactgtcctg catctccaaa aacgcctcga ttttcggctc gatggtttca taattgccgc
     5881 ctttttccgc ataagtgccg ttcagataca tactgatgga tgccggcgaa gtaccgatac
     5941 cgcgtgccag ttgggtttgg ttcatgcctg atttggcttt aaattcagcc agtttttgtt
     6001 gcaatgcttg attaatttgt ttcattttta atatccttga gttttaaaca acctttaaag
     6061 gccgtctgaa atgaaagagc ttcccgattt agaaatttga gcctgattcc gttcagcggg
     6121 aacaccttga atctttactt gaaatcgttg aatccgccca agggctcgct tgagtttcaa
     6181 tttttcaaca agctcggccg aaaacgccgc attttgctgt ttgtccatca tgttttcctt
     6241 tatctatccg cctcaaatag cacaaaatcg tccgtacccg ttttcggcaa taccgcatac
     6301 tccgcctcga tgacgtttcc gcccaaatgt cccagctcgt cccaaaccgc agcctgttcc
     6361 agtgccggat tgacttccgc gtttgcaagc ttgattgcat tttccgcccg cttgattttg
     6421 ccttttcggc gtttttctgc cagttggtcg atacgcgccg tcgggaaagc ctcgcgggta
     6481 ttgccgttgg cctgagcctt agtaataaac ttgccgtcca tatcaaacac attgaccgcc
     6541 gacgcatcgc tcaaatcgta gctgacccgt acctcgtctt tgtgatactc cgccagctcg
     6601 gttgagaaat aagagttgtt gaacaaatcc agccaaccgc gctgtacctt tcgcacttcc
     6661 tgcggcataa acatcgtcgc cagttcttcc gccgacaaca tatccggcgc gataccgtcc
     6721 tgttccagcc tcatttcccg ataagccttc ggcgtataat gcccgccgtc aggatgtcgg
     6781 ggcagctcgc cgtgcgggcg gttgttgtat tcgtcgatac atttgaccac atccgcgata
     6841 aaacgcgacc agcccggcag ttttttcaaa tatttctgtt gttcctccgt caaatccttg
     6901 cctttttcca aagcattaaa agcactttcc atcttgcggt acatcaggtt cttcgtgctg
     6961 ctgtccatcc ccgcgcccgc aaacgtctca tactgccgcg ccatctcaat cagattgtct
     7021 ttccaccatc gctcaatgat gccgcgccct tgcgggttgc ccgcgatacc cgtttcatgc
     7081 cggataccca gtcgggacgt aatacccgtg atttcatggt ctatcgtctt gccggtttgg
     7141 ccaccgccgt tatccgagta atagataatc ggcaaaccaa aatgcttgac cccgatacgc
     7201 agagcgtccg ataccgccac acaactttca gccaacgaaa ccgaaaaacc gaccacaaac
     7261 cgcgtacaac catcaataat caccgtcact tccggcttaa acggcctgcc gtgtaccgga
     7321 tgtgccacct tcgccttaaa gctgtggccg tcgccgatcc aaacatcgtt cggcttcaaa
     7381 gccccccaat cacgtttcac ataaggcagc agcgatttat aagccgcccc cgttttccta
     7441 ccgcgctcct gcataatcag cggcagcttg tcccaaacgc ggcgcaccat actcaagtta
     7501 ggcacatcat tgaccggcat attttccgct tccgcccact gcacaaaccg gcggtagctg
     7561 tgcgccagtt tcggcgcgga cgggatattg tgaaactgca taaacatcgg caaccaaccg
     7621 tagctctcaa tcggcttgac tgtcttcgtc gtcttcggag ccaaagcaac caaccgctcc
     7681 gtcgcgtctt ccgctttcaa ataagcagaa atccagccgt ctaaagtacg ttcgccaacc
     7741 tttgccgtcc ggctgcggtc attggccttt tccaagttcc caagcgtgac cgcgtccaat
     7801 ttaccttctg ccagcaagcc caaaaactga gccaccgcag ccttggcaga gcaatcgtat
     7861 tggtatttaa tccccaacac cgccgccacc accgcacatc gcgcatccgc caccgaccgt
     7921 tgtttctcgt tcaacagctt ggccgcttca gccagtgctt gagccgacat cgccgtcccc
     7981 ggtctgactt ggggcagggt tttcggcatc ttctccgcca gctcgtccga ctgccgtttc
     8041 atgatggctg ctcggatttc ggcagggagg gcatattcaa caccaccacc cttaccttga
     8101 cgccttctcg tttgccaacc atccctttgc gctttttttc tcagccctat tactgttctt
     8161 ggcatatttg gcaacccaaa attaagcagg cttttgagat ccatatattc catttcaacc
     8221 cactccgagc atctctttca attcagctat ctttttgtct cgacaatctg ttttcttgta
     8281 atgcggaaaa acttcaacta aatctttttg aatcactttc gcgattgctt ccgcaattct
     8341 tttgcttcct cgtccatttc ttatgacctc agaaacagat tggttagtta cccctaaagc
     8401 cgaagccaac attcttgccg atatattttt ttcttctaag gatttataga tttcgttcgc
     8461 ttgcataatg cctccatgat agaatccatt acttacttac cttacaggct ttaatgcctg
     8521 ccttaactgt tacttggatg atatacacaa aatatttggt ataccaaata tatttggtaa
     8581 tattttgcga tgtttttatg gaacgcattg atttagcatt gaatttagtt gaagagagaa
     8641 gccgacttgg atactctcga aagaattttg ctgagcaaac tggatgcagt gcagaaagtt
     8701 tgagactgta tgaaagcggg caggtcaaca ttcctgcgga ctttttactt gctgctgtcc
     8761 aattaggggt ggatattcaa tttgtcttta caggcattca ttcggcaaat ttaaatgctg
     8821 tttcagacga ccttaataaa aaattggacg acgctattgg caaatcaaac gttgagaatc
     8881 agattaacgg caacgtcagt aacgctgtta tcgcctcatc tggttcggtt gttcatcaaa
     8941 ttaataccca aaatcacgtc atcagaaccc gtgtcgattc aaaacccggc aaggaacata
     9001 taacgcttga gcaagcttca aagctgcaac aacttgttaa acaagttgcc gcagcagaag
     9061 agattgccaa acggtcgcca aaatcaatcc gtgcaatttg ggctagtctg aatgctcact
     9121 gtaaagttcc aagttacaaa ctaatcgcat tgagcgatta cgacaaagca gaaacttatc
     9181 tcagaaaatg gctgggtagg ttgagcaata cagcaacatc aaagaacaac gatcccgact
     9241 ggcgaaaaaa gaaatacgct tatatcaaat taaatgtaaa acagcttgag cttgaagatt
     9301 ggttgaaaag ttacctcgaa aaaaatttct cagtagaaag cctgactgaa ttaagtgatg
     9361 aagatttgca aaaaacatat gcagcagtat cgactaaaaa acggaaaaaa taggcttaaa
     9421 catgaaactc cgcctttaaa ttcaagtttc atgtttagct tatggtcttc agatgctttt
     9481 ttagatgtat gatattaaaa gattttttca aacatggaac tattatacaa gttccatgtt
     9541 cagtttctag ttaaagcctt ttataaacat tctaatcaac cacgccgaaa ccgagccgcg
     9601 cgacggccta tatgtcttgc gtataggcaa tgacctcttt gtcaaacgtg tgcaacgtat
     9661 accgggcaaa ctgcttgtaa cgtctgaaaa tccacgttac gcaccgtttg aaatagattt
     9721 gagtaatacc caagacgaca tcgccatcat aggccgtgtt gagtggtatg gccggagtat
     9781 tgattaaatc cattttaaaa acctcttaaa acagttttaa aaattcccta atctcgtcag
     9841 ctttaaacag aaaaccgcgc attccggcgc ggttttgtga aaaagctggc gtaacttttc
     9901 cggatacaaa aaaacgccga aatccacgtc ttccgaagat ttcagcgttt ttttactcta
     9961 tttgttcctt gtgcaaaaac taacagtccc ccacaggaaa acgtcgccct gttccgccac
    10021 aacggcgaag ttttattgga cggcatcaac atcaaactca aaagcggcga ttcgctgctg
    10081 atacgcggcc cgagcggttg cggcaaaacc tcgctgctgc gcgcgctggc tgggctttgg
    10141 ccgttcggca gcagcggcaa agtcagccgt ccgccgcatc aagacatcct cttcctgccg
    10201 caacgcccgt acacggcaca gggcagcctg cgcgacgcgg tttgttatcc ggacatcgac
    10261 aaacagcatc ccgagttagc cgaagccatg aacacctgcc gcttgggtta tctgattgac
    10321 aaattggaca aaacagacga ctggcagcac aaactctccc cgggcgaact gcaacgcgtc
    10381 gccttcgtcc gcgccctgct ttccaagccc aaaatcgtcc tgctcgacga agccaccgcc
    10441 gctttggacg aaccgaccga ggccctgctc taccgcgcac tgaaacaaaa actgcccgac
    10501 agcatcatca tcagcatcgg ccaccgcagc acgctcaacg cgttccacaa tatgcacctc
    10561 gatgtcggca acgttgcctg cggttgaacg atgccgtctg aagccgtttt cagacggcat
    10621 ttttcaaatt gataaagcac gcgaaaacaa cgtacaataa acccgtttac cacccctcgg
    10681 aatgccgaac cgtgaaccag cttattttcg actttgccgc acacgactat ccgagtttcg
    10741 acaaattcct cggcacggaa aacgcggaac tggtctatgt cctccgacac aaacacggac
    10801 agtttatcta tgtctggggt gaagaaggtg cgggcaaaag ccatcttttg caggcgtggg
    10861 tcgcacaggc gctcgaagcc ggcaaaaacg ccgcctatat cgatgccgcc tccatgccgc
    10921 tgaccgatgc cgcgttcgag gcggaatacc tcgccgtcga tcaagtcgaa aagctgggca
    10981 acgaagaaca agccctgctt ttttccatct tcaaccgctt ccgcaacagc ggcaaaggct
    11041 ttttgctgct cggttcggaa tacacgcccc agcagctcgt catccgcgaa gatttgcgga
    11101 cgcgtatggc gtactgcctc gtttacgaag tcaagccctt aaccgaccaa gaaaaaatcg
    11161 acgcgctcgc cagtatggcg gcggcacgcc aagtaaccgt cgattccgaa attttcgaat
    11221 acctgctcaa acactggcgg cgcgatatgg acagcctgat gatgatgctc gacacgctgg
    11281 acaactacgc cgtcacaatg ggcaaacgca tcaccctgcc gcttttgcgc cagcttttga
    11341 aacaacagga aacccaatga aaaacctcgc catcttcgac ctcgacaaca ccctcatcaa
    11401 caccgattcc gaccacgcct ggccgcaata cctcatcaaa aaaggcttgg ttgacgctgc
    11461 cgaaaccgaa gcgcaaaacg aaaaattcta ccgcgactac caaaacggct gcctcgacat
    11521 cgacgcattc ctcaaattcc acctcgcccc gctcgctcgt tacagcaaag aagagctggc
    11581 ggaatttcac cgcgaattta tggcggaata catcatcccc cacatctcgc ctatgcagcg
    11641 tatgctggtg cagagccacc aaatggcagg cgacgaaacc cttgtgattt cctcaaccaa
    11701 cgagttcatc atcacccccg tctgccacct tttcggcatc accaacatca tcggcacaca
    11761 actcgaaacc ggttctgacg gccgctacac cggcaattac atcggcacgc ccagcctcaa
    11821 agaaggcaaa atcacccgcc tgaaccaatg gcttgccgaa cgcggcgaaa cgctccaaag
    11881 ctacggcaaa acctattttt acagcgactc caaaaacgac ttgccgctgc tgcgccttgt
    11941 cagcgaaccc gtcgccgtca accccgatgc cgagctggaa aaagaagcca aagaaaaagg
    12001 ctggccggtt ttgaatttca aatgacgcaa tgccgtctga agcccgattc ggcgttcaga
    12061 cggcattgag gctgaaatcc aaaaaactca aagccacagg aatttatccg gaaaaacaaa
    12121 aaacacagga attcatcggg aaaaacaaca atctttccac cgtcattccc acgaaagtgg
    12181 gaatcttgat ccgtccgcac ggaaacttat atttcgtcat ttgcacccga cataattcaa
    12241 tactttattt tgcaataaat taacggatgc tgacaaatta gattggatta attaccctat
    12301 tatccatatt gggttatcgg gttgctaatt gagcagagcg tagcaaacca taaagtgagt
    12361 gtttccaccc actctgcatt tatagtggat taaatttaaa tcaggacaag gcgacaaagc
    12421 cgcagacagt acagatagta cggcaaggcg aggcaacgct gtactggttt aaatttaatc
    12481 cactatacta tgaagtggtt attaaatatg ataatgagac ctattaaatt tagtatggta
    12541 aatacgttat tatttattgt tatatgtagt tcattttttg atctgctcgt tcaattatgt
    12601 acaattttat ttcatagcca aaaaatatac tttattacat tatttttatt atttattttt
    12661 aattttgtta caaaatctat ctatatggca attatttatc ctattttata tttttttacg
    12721 ataaaaaaat attatcctta ctctaggaaa gtgataattc tattattatt agcattatct
    12781 atatatttta gttttatgga cttttacttt ttttccatat attcagataa ccttagctat
    12841 gaaacggagc ctttacattt atacatccct attattatta attttttctc acttttagtt
    12901 tctaatttta ttttatcttt tatcaacaag taaatgtata tacaaaatac aatccaaaag
    12961 atcctaagca aaccaccaat cccacaccac ccttaccggt tcaaatccgc acaaaaaccc
    13021 acatcccgtc attcccacga aagtgggaat ctaggac
//
LOCUS       DAWFAN010000043        12697 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWFAN010000043 DAWFAN010000000
VERSION     DAWFAN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.043"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            332..1645
                     /locus_tag="WGO65_000875"
     CDS             332..1645
                     /locus_tag="WGO65_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ0327188.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1863..2038
                     /locus_tag="WGO65_000876"
                     /pseudo
     CDS             1863..2038
                     /locus_tag="WGO65_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2034..3284
                     /locus_tag="WGO65_000877"
     CDS             2034..3284
                     /locus_tag="WGO65_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0327189.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3319..4812)
                     /gene="rng"
                     /locus_tag="WGO65_000878"
     CDS             complement(3319..4812)
                     /gene="rng"
                     /locus_tag="WGO65_000878"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ0327190.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            5026..5283
                     /gene="grxC"
                     /locus_tag="WGO65_000879"
     CDS             5026..5283
                     /gene="grxC"
                     /locus_tag="WGO65_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ0327191.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5304..5747
                     /gene="secB"
                     /locus_tag="WGO65_000880"
     CDS             5304..5747
                     /gene="secB"
                     /locus_tag="WGO65_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ0327192.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5833..7875
                     /gene="recG"
                     /locus_tag="WGO65_000881"
     CDS             5833..7875
                     /gene="recG"
                     /locus_tag="WGO65_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ0327193.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTATETLEQLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLSELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFANLEEDLHLLEQAREIAPLLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(8592..9635)
                     /locus_tag="WGO65_000882"
     CDS             complement(8592..9635)
                     /locus_tag="WGO65_000882"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ0327194.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIWDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGGIPLLP"
     gene            complement(9632..9913)
                     /locus_tag="WGO65_000883"
                     /pseudo
     CDS             complement(9632..9913)
                     /locus_tag="WGO65_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10165..11319
                     /locus_tag="WGO65_000884"
     CDS             10165..11319
                     /locus_tag="WGO65_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0327195.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDPRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTATQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            11548..11901
                     /locus_tag="WGO65_000885"
     CDS             11548..11901
                     /locus_tag="WGO65_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327196.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            11962..12588
                     /locus_tag="WGO65_000886"
     CDS             11962..12588
                     /locus_tag="WGO65_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ0327197.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRATNLFIS"
BASE COUNT         2941 a         3126 c         3577 g         3053 t
ORIGIN      
        1 ccgccttgtc ctgatttttg ttaatccact ataaaatccc acgtgtacgg cacgccccgc
       61 gctccgcaat gataggggcg gtttcgtttg aatcggcgta ccgggatgaa tgcccccgcg
      121 ccgtcccgat accggtttgc cggttgttcc gtatcggttg ccgccgtgaa atgacggacg
      181 gatacaggaa tggcggcagg cgtaaatggg aggaaatgac cgattgactt gtttcaaaat
      241 aaaaaagtag aatacaaggc ttatcccgcg tctgtgggcg gttttgcctg cacgggcggt
      301 tttctgttta aaataaaata aaggagaaat gatgctgacg tttatcggac tgctgattat
      361 cggggtcatc gtatggttgc tgctgacgga aaaagtgtcg cccatcatcg cattaatctt
      421 ggtgccgctg tttggggctt tgctggcggg gtttgatgta tcccaattaa aagaatttta
      481 ttcgggcggc accaaatcgg tgatgcagat tgtgattatg tttatgtttt ccattttgtt
      541 ttttggaatc atgaacgatg tggggctgtt ccgtccgatg ataggcggtt tgattaagct
      601 gactcggggt aatatcgtgg cagtgagtgt ggggacggtc ttggtgtcgg tggtggcgca
      661 gttggacggg gcgggtgcga cgacgttttt attggtggtc cccgcccttt tgccgcttta
      721 caagcgtctg catatgaatc cttacctgct gtttttgctg ctgacttcca gtgcgggatt
      781 gattaacctt ctgccgtggg gcgggccgac tgggcgggtt gcaagcgtgt tgggcgcaga
      841 tgtgggcgaa ttgtataaac ctttgttgac ggtgcaaatt atcggtgtgg tgtttatcct
      901 tgcgctgtcc ctgcttttgg gtgtgcgtga aaaaaggcgg attgtccggg agttgggcgc
      961 gttgcccgcc gtggcggatt tgataaagcc ggtgcctttg tcggaagaag aacaaaaatt
     1021 ggcgcgtccg aaactgtttt ggtggaatgt cctgctgttt ttggcggcga tgagcctgct
     1081 tttttcgggc atcttcccgc cgggttatgt atttatgctg gctgcaacgg cggcgttgct
     1141 tttgaattac cgcagcccgc aggaacagat ggagcggatt tatgcccacg ccggcggcgc
     1201 ggtgatgatg gcgtccatta ttttggcggc aggtacgttt ttggggattt tgaagggtgc
     1261 ggggatgttg gacgcgattt ccaaagacat tgtgcatatc ctgccggacg cgctgctgcc
     1321 ttatctgcat attgccatcg gtgtgttggg cattccgctt gagttggttt tgagtacgga
     1381 cgcttattat ttcggactgt ttccgattgt ggagcagatt acctcgcagg cgggcgtggc
     1441 gcccgaagca gcaggttatg cgatgttgat cggcagtatc gtcggcactt ttgttacgcc
     1501 gctttcgccg gctttgtgga tgggcttggg tttggcgaaa ttgtcgatgg gcaaacacat
     1561 ccgttattcg tttttctggg cgtggggttt gtcgctggcg atattggcca gttcgatagc
     1621 ggcaggaatc gtgcctctgc cgtaaacggc gaagccgtct gaagcccgaa tcgttcctgt
     1681 ggtctgtcgc aggctctgcc tgcccgtgtg ttgcaatcgc gccccaatgc cgtctgaagg
     1741 cagttgccgg cgcggttttt tgtgccgcag ttttgaaaaa taccggcaag cgtaaaggcc
     1801 gctaaattgc ggctaatttt ggggtgttag aataggcacg gtttattttg aagggaaagt
     1861 tgatgcgtgt tttgctggtg gaagacgatg cgatgattgc ggaggcggtg tcggcaagtt
     1921 tgaaagacgg cggctatgcg gtggattggg tcaaaaacgg cgcgcggctg ccgctgccca
     1981 gccttatgac ttgatgctgc tggatttggg tttgctgggg cgggacggtt tggatgtttg
     2041 ttccgctggc aatgcttgca ggtatgtttt cctactacga aaccttccac gaaacggaag
     2101 cgttgcagga cgacctgctc cgtcaggcgg cattgtacgt cgcacccgat tccaaacccg
     2161 aaactttgcc cgagggcgac ggcgatacgc gcattttcgt gcagatgccg cagcaggaag
     2221 accctgttgt cagcctgcct gcgcatctgg cggacggtct gcacacgctt caggcggacg
     2281 gggacgacga ttattaccgc gtctatatcc gcacgaccga acagggacgg attgccgtta
     2341 tgcaggaaaa cgaataccgt gaagatttgg cggcggatgc ggcacggcaa agcgtgttgc
     2401 ccctgctggc ggcactgccg ctgatgatac tgctgaccgt gtggattacg cacaaagcca
     2461 tgcgccccgt ccgcaaattg tcgcaaagtc tcgaacaacg ccgaatcaat gacctgtctg
     2521 ctttgagtgt ggacaatatt cccagtgaaa tcagagggtt cgtaaccgcc atcaacctgc
     2581 ttttgaaacg tgcggatgaa gacatccgcc accgtcagcg gtttgtcgcc gacgcggcac
     2641 acgaattgcg tacgccgatg acagcccttt cccttcaggc ggaacggctc aacaatatgt
     2701 cgctcccacc cgatgcagcg cggcagtctg ccgttttgca gcagagcatc aggcgcaaca
     2761 aacacctgct cgaacagctt ttggcactgg cgcgttcgca gtcggatgaa acccctctga
     2821 cgaaaacgac attcggtctg caaagccgtt tccgccaagt gttgcaggaa ctgatgccgc
     2881 tggctttgga aaaacgtcag gacatcggcg tggcggtcgg aggcgatgtc gaagtgtctg
     2941 ccgacgaaac ggaaatctat acgctgatta aaacctttgc cgacaacgcg gtacgttaca
     3001 cgccgcccga aggcaggata gatttgggtt tcacggacga agggaaatat ctcgccgtgt
     3061 gggtggaaga taacgggaac ggcattcccg aatccgaacg cgcccgcgtc ctcgatccgt
     3121 tttaccgtat tttgggaacg gagcagcagg ggacggggct ggggctgtcg attgccgaca
     3181 cgcttgccaa aaaatacggc ggacatttgg aattgaccga cagccgacgt ttcggacacg
     3241 gtctgttgat acgggcgttg ttggacaagg aaaccctgaa atagacagat gccgtctgaa
     3301 ggcttcagac ggcatttttt acatcagcac aatatcgtat tgttcctgcg tgtaagcggt
     3361 ttcgaccgcc agagaaatcg gtttgccgat gaaatctatc agcattgcca aggattgcga
     3421 ttcttcgtcc aaaaacaaat cgatgacgtt gggggcggcg aggatgcgga aactttcggc
     3481 atcgtaacgg cgcgcttcgc ggacgatttc gcgctggatt tcgtagcaca cggtttgcgg
     3541 cgttttcaga cggcctctgc cttggcagga agggcagggt tcgcagagga cttggtttaa
     3601 gttttcgcgc gagcgtttgc gcgtcagctc gaccagcccc aggctggtaa aaccgtgcag
     3661 ggtaacgcgg gtacggtcga aggcgagggc tttggcaagc tcctgcaaca cggcttcgcg
     3721 gtggctttcc tgcgccatat cgatgaagtc gatgatgatg atgccgccga ggttgcgtag
     3781 cctcaattcg cgggcgatgg tgtggcaggc ttcgaggttg gtgcggaaga tggtttcgtc
     3841 gaagttgcgt gcgccgacga agccgccggt gttcacgtct atcgtggtca tggcttcggt
     3901 ggattcgata atcaggtagc tgccgaagtt gaggttgacg cgcggttgca gggcgcggct
     3961 gatttcctgt tcgacgttgt gggtttcaaa cagcgggcgt tcgcctttga acaattctat
     4021 cctgcccaat gcgccgtgga cgtattgttc ggcaaaacgc gtcatgcgcc cgtggtttac
     4081 ggtggaatcg acgaggattt tttgcgtgtc gcagccgacc atatcgcgca atacgcgcaa
     4141 actcaagggc aaatcctgat aaagcagggt ttccggcggc cggattttcg cctgttcttg
     4201 gatgtgttcc cacactttgg tcaggtagtc gatgtcggac tggagctgtt cgtcggtggc
     4261 gttttcggcg ttggtgcgga tgatgtagcc ccggcaggca ttttccggca ggagcttgtc
     4321 gaggcgttcg cgcaggctgc tgcgttcggc atcgtcttcg atgcgttggg acacgccgat
     4381 gtggtcttct tgcggaagat ggacgaggaa acgccccgcc agcgagattt gggtggaaag
     4441 ccgcgcgcct ttggtgttga tcgggtcttt gatgacctgc accaaaacag actgcccttc
     4501 aaacagcata tgttcgatgc gctgggtttc ttcggggttg cggcgttgtt cgaggacatc
     4561 gacgatgtgt aaaaacgccg cgcgttccaa gccgatgtcg ataaacgcgc tctgcatccc
     4621 gggcagcacg cggcgcacca cgcccagata gatattgccg accaggctgt gttcgctgtt
     4681 gcgctcgatg tgcagctcgc agatattgtt ttcctccaac accgctacgc gcgtttcctg
     4741 cggcgtgatg ttgaccaata tcgtttcggg cgggcgcgcg atgtctttgg ggatggggag
     4801 tcctgacaac atggtttttc ctgaaaatgt aataaaaata tttttgcacc cgttccgccg
     4861 cgcagcgcgg gtgcaaaaat atttttcaca atgcctatca tactttaaaa aagaaacttt
     4921 gacacattcc gcacatgccg tttgaacgtg catctgttcc gcacttgcca aacaggggaa
     4981 atgcccctat attgattatc attgcaaaac tttcggaaac ccaatatgca gaccgttacc
     5041 atgtacacag gtccgttttg tccctactgc gcgatggcga aaaggctgct gcacgcggca
     5101 ggtgtcggac atatcgacga aatccgtgtc gatgcaagcc ccgaagcctt tgccgaaatg
     5161 cagcagcttt cgggacagcg cagcgtgccg cagattttca tcggcgaaac gcacgtcggc
     5221 ggatttaccg acctctaccg cctccagcag gaaggcgggc tggacggact gctgaaccct
     5281 taacccccaa ctaggaaaac aaaatgagcg aagaactgca acccgtattc agcatcgagc
     5341 gactgtatgt caaagacttg tctttggaag tgccgcacgc gccgcaaatc tttttggaac
     5401 agggcgagcc tgaagtggat atgcgcgttt ccaccggcag ccaaaagctg gaagacggct
     5461 actacaacgt ggacgttacc gtaaccgtta ccgccaagct ggataacgag cgcacgatgt
     5521 ttttgaacga agtaacccaa agcggcatct tccgtttgga aaacatcccc gaagaagatg
     5581 tgcagctgct gctgggcgtg gcgtgtccga acatcctctt cccttacgcg cgcgaagccg
     5641 tttccggtac ggtaacacgt gccggcttcc cgcccgtcct gctcgcgccg attaattttg
     5701 aggcgattta ccaacaacag caggaagccg aagccgccgg ggcttgattc ctgcctgccg
     5761 atgccgtctg aatccgtttc agacggtatt tttgttttcg gataaaatag gtgcgttcaa
     5821 aggaatctgc ctatgatgtc gcccgaaacc cagaaacagc tcaaaatcac cgatgtttcc
     5881 gccaagaagc tcgacaaact caacctccat accgcgtggg atttggtgtt gcacctgccg
     5941 ctgcgttacg aggacgagac gcacattatg ccgattaagg acgcgccgat tggcgtgccg
     6001 tgccaggtcg agggcgaggt tatccatcag gaagtaacgt tcaaaccgcg caagcagttg
     6061 attgtccaaa tcgccgacgg ttccggcagc gtcctttttc tgcgcttcat ccacttttac
     6121 gccagccatc agaaacagac ggcggtcggc aaacgcatcc gcgccgtggg cgaaatcaaa
     6181 cacggctttt acggcgacga gatgattcat cccaaaatcc gcgatgccga gggcggcggt
     6241 ttggcggaaa gcctcacgcc ggtttacccg accgtaaacg gtttgaacca gcccactttg
     6301 cgccgtatta ttcagacggc gttggacgtt acgccgctgc acgacacgct gcccgatgcc
     6361 ctgctgtgcc gtctgaagct gccacacctc gccgaaagcc tgcgtctttt gcattcgccg
     6421 ccgccgagtt tcacgattca tcagctttca gacggcacgc tgcctgcatg gcaacggctc
     6481 aaattcgatg aacttttggc gcaacagctt tcgatgcgct tggcgcgaca gaagcgtatc
     6541 ggcggcacgg cggcggcatt gggcggcgac ggcacattga cccaagccct gcgccaagcc
     6601 ctgccgtttg ccctgaccga tgcgcaagaa aaagttgttt ccgaaatctg ccgcgatatg
     6661 gcgcaaacct acccgatgca ccgcctgctg cagggcgatg tcggcagcgg caaaaccatt
     6721 gtcgccgcct tgtccgcgct gaccgccatc gaatccggtg cgcaggtggc tgtaatggcg
     6781 cccactgaaa tccttgccga acaacatttt attaaattta aacaatggct cgaacctttg
     6841 ggcattgaag ttgtccgcct ttttggcagt ttgcgtaaaa aagccaaaga cgaagccaaa
     6901 gccaaactcg ccgacggcag cgtcaaaatc gccgtcggca cgcacgccct gttttcagac
     6961 ggcgtggcgt ttcacaattt gggcttgacc attgtggacg aacagcaccg tttcggcgtt
     7021 gcccaacgcc tcgccctcaa aaacaaaggg cgcgaagtcc atcagctgat gatgtccgcc
     7081 acgcccatcc cgcgcacgct tgcgatgagt tttttcgccg atttggacgt gtccgtcatc
     7141 gacgaattgc cgcccgggcg cacaccgatt aaaacgcgcc tcgtcaacaa cgtccgccgc
     7201 gccgaagtcg aaggcttcgt cctcggcact tgccgaaaag ggcggcaggc gtattgggtc
     7261 tgcccattga ttgaagagag cgaaaccctg caactgcaaa ctgctaccga aaccctagag
     7321 cagcttcaga cggccttacc cgaactcaac atcggcttgg tacacgggcg catgaaagcc
     7381 gccgaaaagg ccgaagtgat ggcgcggttt tcttcggggg ggctgaacgt cttggtcgcc
     7441 accaccgtca tcgaagtcgg cgtagatgtg cccaatgccg ccctgatggt catcgaacac
     7501 gccgagcgca tgggcttggc gcagctgcac caattacgcg gtcgggtagg gcgcggagcg
     7561 gcagaaagcg tgtgcgttct cctgtttgcc gagccattga gcgaactcgc caaagcgcgg
     7621 ttgaaagtca tctacgaaca caccgacggc tttgaaatcg cccgccaaga cctcaacatc
     7681 cgcggccccg gcgaatttct cggtgcgcgt caaagcggcg ttcccatgct gcgttttgcc
     7741 aatcttgaag aagacttaca ccttttggaa caagcgcgcg aaatcgcccc tttgctgatt
     7801 gaacaaaacc ctgaaatcgt cgaagcccat ttggcacgat ggctttccgg cagggaaggt
     7861 tatttgggcg tgtgagcgaa atgtcgccgg aaatattgga aaaaagtgca acggaatctg
     7921 attttgcatt atttttgaca cattttcatg attttaaatc tcgttattcc caagtgggcg
     7981 gaaatccggc aatgggaagc cgcagagact tatcgggaaa acggcaagcc ctccgccgtc
     8041 attcccgcgc aggcgggaat ctagaaatga aaaaccacgg tgttatcgga aatgattgaa
     8101 accggagaaa tgacggaatc gaagcgtgtg gaaatgtgtc ggaaattgct gaagctggat
     8161 aaactagatt cccgcttttg cgggaatgac gggattttag gtttttggtt tgttttcgcg
     8221 gcaatgatgg gattttaggt ttttggtttg ttttcacggc aatgatggga ttttaaattg
     8281 caggtattta ccgggaaaaa cggcaagccc tccgccgtca ttcccgcgca ggcgggaatc
     8341 tagaaatgaa aaaccacggt gttatcggca atgactgaaa ccggagaaat gacgaaattg
     8401 aagcgtgcgg aaatgtgtcg gaaatcgctg aagccggata aactagattc ccgcttttgc
     8461 gggaatgacg ggattttagg tttttgtttt cgcggaataa tgagccaaaa gccgtctgaa
     8521 cctaaaaaac cgccgctttc agatggcatc aaaaaccgca ggcacgatgc ctgcggtttg
     8581 ccgtttgcgc ttcaggggag cagggggatg ccgcccaagc cgtgtgtttc cttcagtccg
     8641 aacatgatgt tcatattttg caccgcctgt cccgccgcgc ctttgacgag gttgtcgatg
     8701 acggaaagga cgacccacac atcggattgc gccgcctgtt ggatgctgat gcggcagagg
     8761 tttgcgccgc gcacgctgcg ggtttcgggt gtcgaaccgg cgggcaggat gtccacgaac
     8821 aggctgtcgc ggtaatagtc gcgcaggatg gtttcggggc agatgccgtc tgaaaggtgg
     8881 aggtaaacgg tggcgtgcat accgcgtatc atcggcgcga ggtgcggcgt gaacacgaat
     8941 ccttcggcga tgccgtcctg aagcccggcg atggtctgcc tgatttcggg caggtggcgg
     9001 tgtccagcta tgccgtaggc tttgaagttg tcgccgactt cgcacaacag cgaaccgaca
     9061 ttgcctttcc tgcccgcgcc ggacacgccg gatttgcagt cggcaatcag cggcataccg
     9121 ggcttcagac ggcattgccg caacagcggc acgagcggca gggatacgca ggtcgggtag
     9181 cagccggggt tggcgacgag gcgcgcctgt gcgacggctt cgcggttgag ttcgctcaat
     9241 ccgtacacgg cttgggaaac gaggccgggg gcggcgtggg tcatgccgta ccagtgttcc
     9301 caggtcggaa tgtcccatat gcggaagtcg gcggaaaggt cgatgacgcg cacgccctgt
     9361 tcaatcaggc gcggcgcgtc tttcatggcg atgccgttgg gcgtggcgaa gaagacgatg
     9421 tcgcattgtt ccaaacctgc ctcgtcgggc gtttggaagg cgaggccgta cacgccgcgc
     9481 aaactcggaa agtaatcggc aactgcggtt cccgcttcgc tgcggctggt tacggcggcg
     9541 acttcgacat cgggatgggc ggcaagcagg cgcagcagtt ccacgccggt gtagcccgtc
     9601 gcgccgacaa tgccgacttt gattttttta ctcatggttt ttcctttgtg tggttggcgg
     9661 gtatgccgtc cgaacgctgt ccgacggggt tttgattggc ggcggaagga cggtacacaa
     9721 agcgtttgaa aagcgtgtct aatttaatgg catccgcctc tgtttttaaa acctagccct
     9781 gccgtccgga ataaacataa ccgtaggcgc gaaagccggc aggatatttc ggtgcctgcc
     9841 ccaaacccaa accgtatgcg gcgaaagcgg tggtcgtgga gaaggaaagc aggaggagga
     9901 cgagggtttt cataaggttc caaaccgaac ggggcgatga agcccgatta tagcaaaagg
     9961 tcgtctgaaa accgttttca gacgaccttt tatttaatta caataaaaat gttaacaaca
    10021 aaaaacaaac cgcttttttc cgtttggaga caaattttta actaaaattg tcaacaattc
    10081 cttgatgcac acaaaaaaaa ctttaatatt tgtaaatata tgtaacatta taaattttaa
    10141 atattttctg agtaagggga agtaatgagc atccatctcg acttcggcat tagtcctaaa
    10201 acgttccgac agacttatct gtatcaaaag cccaagctct ttaaaggagc ggttcggaat
    10261 ctcgaatccg catcttggaa agatatcaac gagatatacc aacgagcaga cccaaccgca
    10321 ccgctgtttc atctgcgtaa aaaaggcgca atcgttccta aagaagaata cgtcgaaagt
    10381 ttcgacgatt tgggcaaaac tcgctaccgt tttattaaat ccgttatcta cgaacatatg
    10441 aagaatggtg cgtcgttagt ctataaccat attaacaacg agccgttttc agaccatatc
    10501 gcccgtcaag tcgcccgctt tgccggcgca catactattg ttagtggata tcttgctttt
    10561 ggcagcgacg aatcttataa aaaccattgg gacccccgcg atgtgtatgc catccagctt
    10621 ttcggcaaga aacgttggca acttactgcc cctgatttcc ctatgccatt gtatatgcaa
    10681 cagactaaag atactgatat ttccattcct gaacatatcg atatggatat tatccttgaa
    10741 gcaggtgatg tcctctacat cccacgcggt tggtggcaca gacctatccc gctcggctgt
    10801 gaaaccttcc acttcgctgt cggtaccttc ccaccaaacg gctataatta cctcgagtgg
    10861 ctaatgaaga aatttcccac catagaaagt ctgcgccaca gtttctcaga ctgggagcaa
    10921 gataggacgc gtatcaacga tactgccaca caaattgctg ccatgattgc cgaccccgtc
    10981 aattatgaag ccttcagtga agactttctc ggcaaagaac gtaccgatac cgcttttcat
    11041 ctcgaacagt tcgcgaatcc caacgctact ccgctttcag acgacgtcag gttgagatta
    11101 aatgccaata atttggatac gttggaaaag ggatatttga ttgggaatgg gatgaagata
    11161 agcgtagatg agttggggaa aaaagtgtta gaacacatcg gtaagaatga accgttattg
    11221 ttgaaaaatc tactggttaa cttcaatcag ggaaaatatg aagaagttag gaagttgatt
    11281 tatcagttga tagagttaga ttttctggaa attttgtgag ggattctatg aaaaactgga
    11341 agcaatttac atttttcgta atattagtaa tagcttgtta tcaagtgcta tattttttat
    11401 cagatatgtt tcttctcgac tacattaaca aatatagttg gaatttgaat tttattcagg
    11461 gtactttgaa ttttttttca atatatctgc catatgtttt tgttagccga atttttagga
    11521 ataccaacca agaaaaggag tataaaaatg attgaacttc aacttcatga attgaagctg
    11581 gtttcagggg gaggtcctgt aacagacaat atagctggaa atgtagctaa tgctgccaca
    11641 accaaaggag gtcccacatg gggggatttg gttgcaatac ctgctgccgc agcggcagtt
    11701 tattatctgc cgaaaaatgc ttacggtgct gctggtgcaa atggtgtata caatgtgact
    11761 cgtaattggg taaatgatgc cgttaatgca cctccttata acggaagacc aatctttgag
    11821 attgaacatg gattaactgc tcccgcaaca aaagcagata aatcaggaaa cggctacact
    11881 gacggtacag attactgctg atatttctca tcgtccagac aatctctagg ggtcgtctga
    11941 aactttctta acttcaattt tatgaataga cccaagcaac ccttcttccg tcccgaagtc
    12001 gccgttgccc gccaaaccag cctgacgggt aaagtgattc tgacacgacc gttgtcattt
    12061 tccctatgga cgacatttgc atcgatatct gcgttattga ttatcctgtt tttgatattt
    12121 ggtaactata cgcgaaagac aacagtggag ggacaaattt tacctgcatc gggcgtaatc
    12181 agggtgtatg caccggatac ggggacaatt acagcgaaat tcgtggaaga tggagaaaag
    12241 gttaaggctg gcgacaagct atttgcgctt tcgacctcac gtttcggcgc aggaggtagc
    12301 gtgcagcagc agttgaaaac ggaggcagtt ttgaagaaaa cgttggcaga acaggaactg
    12361 ggtcgtctga agctgataca cgagaatgaa acgcgcagcc ttaaagcaac tgtcgaacgt
    12421 ttggaaaacc agaaactcca tattttgcaa cagatagacg gtcagaaaag gcgcattaga
    12481 cttgcggaag atcagtggca aaaacggcac tgggcagaca ggaattgtcg ggattgggca
    12541 tggtatcctc cgatttggcg aagagcaacg aacctgttta tctcgtgaaa ataaaacccg
    12601 acaaaccaac catcactgca tacggtgagg aaaaaccgct gcaaatcggc atgacgttgg
    12661 aagcagacat cctacacgag aaacggcgat tgtacga
//
LOCUS       DAWFAN010000044        12470 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWFAN010000044 DAWFAN010000000
VERSION     DAWFAN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.044"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..206
                     /locus_tag="WGO65_000887"
     CDS             <1..206
                     /locus_tag="WGO65_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEZ0327198.1"
                     /translation="PRGAGGEIQLTDGIARLLDHEFVLAHAFEGKRYDCGSKLGYLEA
                     TVAYGLKHPELKDDFKELLKQFV"
     gene            289..1305
                     /gene="galE"
                     /locus_tag="WGO65_000888"
     CDS             289..1305
                     /gene="galE"
                     /locus_tag="WGO65_000888"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0327199.1"
                     /translation="MNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPRL
                     KTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPIKYYDNNVS
                     GSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERIL
                     TDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAVF
                     GDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRAF
                     EAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNHPN
                     GYDD"
     gene            1401..2462
                     /gene="rffG"
                     /locus_tag="WGO65_000889"
     CDS             1401..2462
                     /gene="rffG"
                     /locus_tag="WGO65_000889"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0327200.1"
                     /translation="MKKILVTGGAGFIGSAVVRHIINDTQDAVVNVDKLTYAGNLESL
                     ESLANHPRYAFEQVDICDRAALDRVFAEHQPDAVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWHDMPSEKKAAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGEIGETYNIGGHNEKANIEVVRTICALLEELVPN
                     KPQGVARYEDLITYVKDRPGHDVRYAIDAAKIGRELGWQPQETFESGIRKTVEWYLNN
                     QTWWSRVLDGSYNRERLGS"
     gene            2675..3550
                     /gene="rfbA"
                     /locus_tag="WGO65_000890"
     CDS             2675..3550
                     /gene="rfbA"
                     /locus_tag="WGO65_000890"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0327201.1"
                     /translation="MKGIILAGGSGIRLYPITRGVSKQLLPVYNKPMIYYPLSVLMLA
                     GIRDVLVITTPEDNDAFRRLLGDGSDFGIQISYAIQPSPDGLAQAFLIGEEFIGDDSV
                     CLVLGDNIFYGQHFSQSLKEAAASVETKGATVFGYQVKDPERFGVIEFDENFKALSIE
                     EKPKQPKSDWAVTGLYFYDNRVVEFAKQVKPSERGELEITTLNQMYLDDGSLNVQLLG
                     RGFAWLDTGTHASLLEAASFVETIENIQNLQVACLEEIAWRNGWLSSEEIRNLAKPMC
                     KNEYGRYLLRLVGKD"
     gene            3605..4144
                     /gene="rfbC"
                     /locus_tag="WGO65_000891"
     CDS             3605..4144
                     /gene="rfbC"
                     /locus_tag="WGO65_000891"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0327202.1"
                     /translation="MNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERTF
                     VQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVLS
                     ADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLGE
                     PSLSAKDLAGKMLAEAATF"
     gene            4306..6354
                     /locus_tag="WGO65_000892"
     CDS             4306..6354
                     /locus_tag="WGO65_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ0327203.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPEINRL
                     VQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSIYFNAETPSRLEYILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYR
                     NLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQY
                     ADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGCFAKKLGKIKQLYRSFK
                     "
     gene            6491..7750
                     /locus_tag="WGO65_000893"
     CDS             6491..7750
                     /locus_tag="WGO65_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ0327204.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKNTSDSSTPTT"
     gene            8119..9525
                     /locus_tag="WGO65_000894"
     CDS             8119..9525
                     /locus_tag="WGO65_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ0327205.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            9743..10957
                     /gene="gltS"
                     /locus_tag="WGO65_000895"
     CDS             9743..10957
                     /gene="gltS"
                     /locus_tag="WGO65_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ0327206.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            11056..12072
                     /locus_tag="WGO65_000896"
     CDS             11056..12072
                     /locus_tag="WGO65_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0327207.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            12083..12280
                     /locus_tag="WGO65_000897"
     CDS             12083..12280
                     /locus_tag="WGO65_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327208.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            12298..12447
                     /locus_tag="WGO65_000898"
     CDS             12298..12447
                     /locus_tag="WGO65_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327209.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTRRTIYKAL
                     PYVPS"
BASE COUNT         3190 a         3300 c         2982 g         2998 t
ORIGIN      
        1 tacctcgtgg tgcaggtggt gaaatccagc taactgatgg tattgcccgc ctacttgacc
       61 atgaatttgt cttggcgcat gcgtttgaag gtaagcgtta tgattgtggc agtaagttgg
      121 gctatctgga agccacggta gcttatggct tgaagcatcc cgaattaaaa gatgatttca
      181 aagaattatt gaagcaattt gtataatttt aaggccgtct gaaaaagaaa aggcatcatg
      241 ttttttacac tttcaggcgg ccttttaaac ataaagaagg aaaacgtgat gaacattctc
      301 gttaccggcg gcaccggttt tatcggctcg cacaccgttg tttctttgct gaaaagcggt
      361 catcaagtcg tgattttgga taacctatgc aattccagca tcaatatcct gccacgcttg
      421 aaaacgatta ccggccaaga gattccgttt tatcaaggcg atatccgcga tcgtgagatt
      481 ttgcgccgta tttttgcgga aaaccgcatt gattcggtga ttcattttgc cggcttgaaa
      541 gcggtgggtg aaagtgtggc cgagccgata aaatattatg ataataatgt ttccggcagc
      601 ttggtgttgg cggaagaaat ggcgcgtgcg ggcgtgttta gcattgtgtt cagttcttcg
      661 gcgacggttt atggcgatcc gggcaaagtg ccttataccg aggatatgcc accgggcgac
      721 accaccagcc cttacggcgc atcgaaatcg atggttgagc gcattctcac tgacattcaa
      781 aaagccgatc cgcgctggag catgattttg ttgcgttatt tcaatccgat tggcgcgcat
      841 gaaagcggtt tgattggcga gcagccaaac ggtatcccaa ataatttgtt gccttatatc
      901 tgccaagtgg cggcaggcaa actgccgcaa ttagcggtat tcggtgatga ctaccctacc
      961 cctgatggca cgggtatgcg cgactatatt catgtgatgg atttggcaga aggccatgtc
     1021 gcggctatgc aggcgaaaag caatgtagca ggcacgcatt tgttgaactt aggttccggc
     1081 cgcgcttctt cggtattgga aatcatccgc gcatttgaag cggcttcggg tttaaccatt
     1141 ccatttgaaa tcaaaccgcg ccgcgccggt gatttggcgt gcttctatgc cgacccttct
     1201 tatacgaagg cgcaaatcgg ctggcaaacg cagcgtgatt taacccaaat gatggaagat
     1261 tcatggcgct gggtaagtaa tcatccgaat ggttatgatg attaagtcat gaaattgaga
     1321 ggccgtctga agtttcagac ggcctttgtt tgcgatttat agtaaaattt accttatcat
     1381 tttggcgatt aatattcatc atgaaaaaaa tccttgtcac cggcggtgcc ggttttatcg
     1441 gttcggcggt tgtacgccat atcatcaacg atacgcaaga tgcggtggtg aatgtggata
     1501 agctcacgta tgcgggcaat ttggaatcgc tggaatcttt ggccaatcat ccgcgctatg
     1561 cgtttgagca agtcgatatt tgcgaccgcg cggcactgga tcgtgtgttt gccgaacacc
     1621 agccggatgc ggtgatgcac ttggcagcag aaagccatgt tgaccgcagt attgatggcc
     1681 cagcggcatt tattgaaacc aatattgttg gtacttacac gcttttggaa gcggcgcgcg
     1741 cgtattggca tgatatgccg tctgaaaaaa aagcggcttt ccgtttccat catatttcga
     1801 ctgatgaagt gtatggcgat ttgcacggca cggatgattt gtttaccgaa accacgcctt
     1861 acgccccttc cagtccttat tcggcttcca aagcctccag cgaccatttg gtgcgcgcat
     1921 ggctgcgtac ttacggcctg ccgaccattg tgaccaattg ttctaataac tacggccctt
     1981 atcattttcc tgaaaaactg attccactga tgattctgaa tgcgcttgat ggcaaaccgc
     2041 tgccggtgta tggcgacggt atgcagattc gtgattggct gtttgttgaa gatcatgcgc
     2101 gtgcgctgta tcaagtggtc acagaaggcg aaatcggcga aacttacaac attggtggcc
     2161 acaatgaaaa agccaatatt gaagtggtgc gcaccatttg cgccctgctg gaagaattgg
     2221 tgccgaacaa accgcaaggc gtggcgcgtt atgaagattt gattacctat gtcaaagacc
     2281 gccccggcca tgatgtgcgc tatgcgattg atgccgcaaa aatcggccgt gagttgggtt
     2341 ggcagccgca agaaactttt gaaagcggca tccgcaaaac agtggaatgg tatttaaaca
     2401 atcaaacttg gtggtcgcgc gtgttagacg gcagctacaa tcgtgaacgc ttaggcagtt
     2461 aaatagagac ctttgcaaac tccccatctt tggcacattt cttcgttatg cgctgctcga
     2521 aagcttgcct atctttgtga tatgtctgcg ctttctgcgc tgctataact tcgaactgta
     2581 ctcaaatctg tggctttgca aaggcctcaa ataataaaaa tacgttcaga cggcatgaag
     2641 gccgcctgaa aaactacata aggaaagtaa actgatgaaa ggcattattc tcgcaggcgg
     2701 cagcggtatc cgcctttacc cgattacccg cggcgtatca aagcaattac taccggtgta
     2761 caacaaaccg atgatttatt accccttgtc ggtgttgatg ttggccggaa tccgcgatgt
     2821 gctggtgatt accacccccg aagacaacga tgctttccgc cgcttactgg gtgatggcag
     2881 cgatttcggt attcaaattt cctacgccat ccaacccagc cccgatggtt tggcgcaagc
     2941 ctttttaatt ggtgaagaat ttatcggcga tgattcggtg tgtttggtgt tgggcgacaa
     3001 tattttctat ggccagcatt tcagccaatc cctgaaagaa gccgccgctt ccgtggaaac
     3061 caaaggtgct accgtattcg gctatcaagt gaaagatccc gagcgtttcg gtgtgattga
     3121 gtttgatgag aatttcaaag ccctgtcaat cgaagaaaaa ccgaaacaac ctaaatccga
     3181 ttgggcggta accggcctct atttctacga taatcgcgta gtcgagtttg ccaaacaagt
     3241 gaagccgtct gaacgcggcg aattggaaat taccaccttg aatcaaatgt atttggacga
     3301 tggttcgctc aatgtccaat tgctcggtcg cggtttcgca tggcttgata ccggcacaca
     3361 cgccagcctg ttggaagcag cctcgtttgt cgaaaccatt gaaaacattc aaaacctaca
     3421 agtcgcctgt ttggaagaaa tcgcatggcg taatggctgg ttgagcagcg aagaaatcag
     3481 aaacttggca aagccaatgt gtaaaaatga atatggccgc tatttattgc gcttggttgg
     3541 caaagattaa gaggttgctt ttaccagttt tcaggccgtc tgaaacgctt tttaggaaaa
     3601 catgatgaat atcatcgata cccgcattcc cgatgtcaaa ttgctcgaac ccaaagtctt
     3661 cggtgacgag cgtggttttt ttatggaaac cttccgtgat gaatggttcc gccaaaatgt
     3721 agccgagcgc actttcgtgc aggaaaacca cagcaaatcc acccaaggcg tattacgcgg
     3781 tttacattat caaaccgaaa atacacaagg caaactggtg cgcgtggtat caggcgaagt
     3841 ctttgatgtt gccgtggata tgcgtcgtga tagtcctact ttcggccact gggtcggtga
     3901 ggttttatcg gcagacaaca aacgccaatt atgggtgccg gaagggtttg cccatggatt
     3961 ttatgtgttg agcgatgaag cggaattcgt ctacaaatgc accgactatt acaacccgaa
     4021 tgcggaacat tccctaattt ggaatgatgc cacagtggga atcgactggc ctttattggg
     4081 agaaccaagc ctatcggcca aggatttggc aggcaagatg cttgctgaag cggccacatt
     4141 ttaattttca cttttaggtc atcgtgggta atttgtagca agatgaccta atttttacca
     4201 gctagcctac taactaaatt tctactatat ttaaatagtc agaaaattta cacaaaatct
     4261 gttaataaga tattatattg tcagtaatat tttttcctat ttattgtgat tcctgcctac
     4321 attcctcttc gctctttcgg tatacgtaaa attccgaata ttacgcaatt tctaccggaa
     4381 tattcactta acaaaaccgt attcaacgcc caaaccatca ttggctgggg cttacgcccg
     4441 accacccacc aagcacgtgc ttttgcacaa tcacatggct tgccgtttgt agccttggaa
     4501 gacggttttt tacgctcact gggcttaggc gtggccggtt atccgccctt cgcactggtt
     4561 tacgacgata tcggcatcta ttacgacaca acccgcccat cgcgtttgga gcagttgatt
     4621 ttagccgcaa acatgccgtc tgaaatgctt aaccaagcgc agcaggccat tgatttgatt
     4681 ttgacgcacc gtttgtctaa atataaccat gctctggatt tttcagacgg cctcgtacaa
     4741 gacagcaaaa acattgtttt ggtaattgat caaactttcg gcgatatggc gattcaatat
     4801 ggcggcgcag atgaagccac attccgccaa atgtttcaga cggccttagc cgaaaatcct
     4861 gatgcggaaa tctgggtcaa aacccatccc gatgtattga gcggcaagaa acagggttac
     4921 cttactgatt tggcacagca gccgcgtgtg cgtttattgg ctgaagacat taatccgatt
     4981 tcgctgctgc aagcggtgga caaagtgtat tgcgtgacct cgcaaatggg ttttgaagcc
     5041 ttgttgtgcg gcaagccttt ggtaacgttt ggtttgccgt ggtatgccgg ctggggtgta
     5101 agcgacgacc gccatcctga aatcaaccgc cttgttcaaa cccaacgccg cgccacccgc
     5161 aacttgctgc agctcttcgc cgcagcctat ctgcaataca gccgctacct caaccccaat
     5221 accggcgaag caggcagcct ctttgatgtc atcgactatc tggcgacggt caaacgtaaa
     5281 aacgacaaat tgcgtggcga gttatattgc gtcggtatgt ctttgtggaa acgcgcggtt
     5341 gccaaaccgt tctttaacgt accctcttgc cgtctgaaat ttatctcttc cacccaaaaa
     5401 ctggcaaggg tcaaactgtc cgacgatgca cgcatcctgg cttggggcaa cggcaaagag
     5461 gccatcgtcc gctttgccga acaacaccac atccccctgc tgcgcatgga agacggcttt
     5521 atccgctcgg tcggactcgg ctccaactta gtgccgccgc tgtcgctcgt taccgacgat
     5581 atgagcattt atttcaatgc cgaaaccccg tcccgtcttg aatacatcct acaaaaccaa
     5641 aacttcgacg atcaagactt tcagacggcc ttgaagctgc aaaaaatgct gaccgaaaac
     5701 cacatcagca aatacaacgt cggcagctca gacttcaccg ccccgtcaac cgacaaaacc
     5761 gtgatcctcg ttcccggcca ggttgaagat gatgcgtcta tccgctacgg ttcgccccaa
     5821 atctaccgca atctggattt gctccgtacc gtacgcgaac gaaaccccaa tgcctatatc
     5881 atctacaaac cgcatcccga tgtagtcagc ggtaaccgca tcggccatat ttcccctgaa
     5941 gatgctgcac gatatgccga ccaaaccgcc gaacaagccg acatcctgac ctgtctccaa
     6001 tacgcagacg aaatacatac catgacttcg ctgaccggtt ttgaagcctt gttgcgcggc
     6061 aaaaaagtca gctgctacgg cctgcctttt tacgcaggct gggggcttac ccaagatctg
     6121 ctccccatcc cgcgccgtag ccgcagactt gagctttggc agctgattgc cggcacgctc
     6181 atccactatc ccgactacat ccaccccgaa acccatcagg ccataaatgc agaaaccgca
     6241 gcccaaatcc tgatacgaca aaaaaatatg caaaaaaaca acaacggatt gcatcgcggg
     6301 tgctttgcca aaaaattagg taaaatcaaa caactatatc gatctttcaa ataaatacca
     6361 tcaaagttaa cgatgcgtca taaacttgcc tctattgcgg catcattgcc tttgcatcgt
     6421 taattctctt ggcgtatgct tgaaagttca acctaaaact attacataaa aaacaaaacc
     6481 acattgcaac atgaaacaga ccgtcctcaa aaataacctg caaaacctgc ttgaaagcgc
     6541 agaaaatatc ctgctgcttc aaggccctgt cggcgatttt tttctgcgcc ttgccgactg
     6601 gctgactgca aacggcaaaa ccgtacataa attcaacttt aatgcaggcg acgactattt
     6661 ttatccgccc actcaagcgc ataccgttgt ttttaacgac aactacgatg cctttcctga
     6721 gtttttgcaa gaatacatca ctcaacatca catccaggcc gttgtctgct ttggcgacac
     6781 acgcccttat cacgtcattg caaaacgcat tgcaaacgaa aaccaagcca gtttctgggc
     6841 gtttgaagaa ggctatttcc gcccctacta catcacctta gaaaaagacg gcgtcaacgc
     6901 attttccccg ttgccgcgcc gtgccgactt ttttcttgaa caattcccta agcttgccca
     6961 gcaagaatat aaagcgccaa cgccggtaca cggcggtttt acgcccatgg caaaaaacgc
     7021 tatccgttac tatatcgagt tgttccgcaa tccacgcaaa taccccgact acatccacca
     7081 ccgcgcaccc aatgccggcc attacctcaa accgtggtcg ctctccatcc tcaagcgttt
     7141 gaactactat attgaagaca tccaaatcgc caaacgtgtg gaagcaggca aatacggcaa
     7201 gttttttatt gttcccttac aggtattcaa cgacagccaa gtccgtatcc attgcgactt
     7261 tcccagcgtc cgcagcttcc tgctccatgt tttgagttca tttgccgagc acgcgcctgc
     7321 cgataccaac atcatcatca agcatcatcc tatggaccgc ggctttatcg actactggcg
     7381 cgacattaaa cgctttatca aagaacaccc cgaactcaaa ggccgtgtga tttatgtcca
     7441 tgatgtcccc ctgcccgttt tcctgcgcca cggtctcggc atggttacca tcaacagcac
     7501 cagcggcctg tccggactga ttcacaatat gccagttaag gttctcggcc gtgcctatta
     7561 tgatattccc ggcattactg accaaaatac cttggcagaa ttttggaatc atccgacacc
     7621 gcctgacaaa gagctgttcc atgcctaccg aatgtaccac ctcaacgtga cccaaattaa
     7681 cggcaacttc tacagtcagg tcttttttcc taacaaaaac acatccgact cttccacacc
     7741 aacaacctaa cttggcgaaa gaaaagctca ggccgtctga aaacatttca gacggcctga
     7801 aacaatcaat accttagcta ctgccatgta aataaaacac aaaaatctgc atttatcatt
     7861 aacaataaat tacaaaaaca gtataatgac cgagctgcca tgagcgcata ccgactcaac
     7921 ctgagccctt tgtaacacac aaaatatgga tatatcccta ggcaaaacaa tataacaagc
     7981 caaacatcct aaagataagc cggcaaggca atacactcta taaaactgtg ccgagcaaaa
     8041 tttttacaaa gccctcaacc ggtatcgccg cccatatgcc gcagcatccg tcttccactt
     8101 tatatccgcc cgcaaaccat gaccgccgct cctgatatcc tctaccggca agccgccgcc
     8161 cttttggaac aatccaatac cgcccaagcc ctgcccctgt tgcaacaggc ggcagagcaa
     8221 ggttatgcgg aagctgcttt cgtattgggc aaccatctgc tgcaaaacgg ccaaccggag
     8281 caggcacttt catggttgga agccgccgcg gcccaacgcc atcccaaagc actcttctcc
     8341 ctgctgcaac aacgcgaaca caacggcacc ccgaccggac agcttctcaa cgactatgcc
     8401 tggctgggtg agcaggggca ctcagaagcc caattaatcc tcatgcgtta ccacgcgcaa
     8461 cgcaacgatc cacaatcgct ctactgggcg gaacttgctg ccgcccgata tgccgcacct
     8521 gcgtattacc atctggcacg ccatcatcaa cgccaaggcg acgttgaaac agccatcgaa
     8581 caatacgaaa aagcggcagc actcggcgta actgccgcct gctggcaact tggtcaaatc
     8641 tacttctacg gtacaggtgt cagccccaac cacgcacaag ccgaacacta tctcgaacca
     8701 gccgcacaag ccggccacat cgccgcacaa acgctgctgg ctgaccttct tgccgcccaa
     8761 cgcaaacctg aagccttgga atggtatcgt cgtgccgccg ataaggaaca agcggaagca
     8821 cagtctaagc tggcccaata cgccctgacc ggcgaacttt ccgaacgcga tccgttccaa
     8881 gcggcacgat atgccaaagc cgctgccgag aaaaaccatc ctgaagccct gaaaatcatg
     8941 ggcgacctct accgctacgg tctcggtatc aaagccgaca accatatcgc gcaagattac
     9001 taccaccgtg ccgccgcgct gggttctgcc gccgcagcac aaaaactcat cagcgacgcc
     9061 gcgctgtacc atccgcaaca atacgaacaa atcaaaactg ccgccctgca acaacaacaa
     9121 accgaaacca tctaccgttt ggcggaagca caagcctgcg ccatcggccg tcccgccgac
     9181 tacaatgccg cgcgaaaaaa ttacatggaa gctgccgggt tccaccataa aaacgcagcg
     9241 gcagccttag gccgcatcta ccattacggc ctcggtacgg cgcaagatcc tcgggcggct
     9301 gcacactggt acgccattgc tgccgaacaa aaccaccctt ccgcccaata ccacctcgcc
     9361 tgtttttact atcacgggca aggtgtcggc tgtcatgttc cgaccgcctg ctactggctg
     9421 caggccgcca tcggcaacgg ccacacttcg gccgaatcat taatatccct attagaacaa
     9481 tggcgacgcg aagcacacca tgccatcgga caaaaggccg tctgaaaaga tttacactcg
     9541 cattttttga caatctttaa ctattcccct aatatttgcc agttattttt cacggacacg
     9601 ccattgtttt catttctttc tgaaaacacc ttgtccgcgc atcaatacca tgacactcgg
     9661 cggataacgc caagcgttga aacacactac atccggaaca aaaacggatg ctcggaaaaa
     9721 tatttctagg aggtgaaaca acatggaatg ggaattcaac agttattaca cactgattgc
     9781 cgccacgctc gtgttgctgg ttggtaaatt tctggttcaa aaaatcaaat tcttacgaga
     9841 cttcaatatt cccgagccgg tcgctggcgg tttggttgcc gcgattatcc tgtttgcgct
     9901 gcacgaggcg tacggcgtga gcttcaaatt tgagaaaccg ctgcaaaatg cgtttatgct
     9961 gatttttttc acgtccatcg gcttgagcgc ggatttttcc cgtttgaagg cgggcggttt
    10021 gccgctggtg gtttttaccg cgattgtggg cggatttatc ttggtgcaaa actttgtcgg
    10081 ggtcggactg gctacggctt tgggtttgga cccgctcatc ggtctgatta ccggttcggt
    10141 gtcgctgacg ggcggacacg gtacgtcagg tgcgtgggga cctaattttg aaacgcaata
    10201 cggcttggtc ggcgcaaccg gtttgggtat tgcatcggct actttcgggc tggtgttcgg
    10261 cggcctgatc ggtgggccgg ttgcgcgccg cctgatcaac aaaatgggcc gcaaaccggt
    10321 tgaaaacaaa aaacaggatc aggacgacaa cgcggacgac gtgttcgagc aggcaaaacg
    10381 cacccgcctg attacggcgg aatctgccgt tgaaacgctt gccatgtttg ccgcgtgttt
    10441 ggcgtttgcc gagattatgg acggcttcga caaagaatat ctgttcgacc tgcccaaatt
    10501 cgtgtggtgt ctgtttggcg gcgtggtcat ccgcaacatc ctcaccgccg cattcaaggt
    10561 caatatgttc gaccgcgcca tcgatgtgtt cggcaatgct tcgctttcgc ttttcttggc
    10621 aatggcgttg ctgaatttga aactgtggga gctgaccggt ttggcggggc ctgtaaccgt
    10681 gattcttgcc gtacaaaccg tggtgatggt tttgtacgcg acttttgtta cctatgtctt
    10741 tatggggcgc gactatgatg cggcagtatt ggctgccggc cattgcggtt tcggcttggg
    10801 tgcaacgccg acggcggtgg caaatatgca gtccgtcacg catactttcg gcgcgtcgca
    10861 taaggcgttt ttgattgtgc ctatggtcgg cgcgttcttc gtcgatttga ttaatgccgc
    10921 gattctcacc ggttttgtga atttctttaa aggctgattt tccgcctttc cgacaaagca
    10981 cctgcaaggt ttaccgcctg caggtgcttt tgctatgata gccgctatcg gtctgcaccg
    11041 tttggaagga acatcatgta tcggaaactc attgcgctgc cgtttgccct gctgcttgcc
    11101 gcttgcggca gggaagaacc gcccaaggca ttggaatgcg ccaaccccgc cgtgttgcaa
    11161 ggcatacgcg gcaatattca ggaaacgctc acgcaggaag cgcgttcttt cgcgcgcgaa
    11221 gacggcaggc agtttgtcga tgccgacaaa attatcgccg ccgcctacgg tttggcgttt
    11281 tctttggaac acgcttcgga aacgcaggaa ggcgggcgca cgttctgtat cgccgatttg
    11341 aacattaccg tgccgtctga aacgcttgcc gatgccaagg caaacagccc cctgctgtac
    11401 ggggaaaccg ctttgtcgga tattgtgcgg cagaagacgg gcggcaatgt cgagtttaaa
    11461 gacggcgtat tgacggcagc cgtccgcttc ctgcccgtca aagacggtca gacggcattt
    11521 gtcgacaaca cggtcggtat ggcggcgcaa acgctgtctg tcgcgttgct gccttacggc
    11581 gtgaagagca tcgtgatgat agacggcaag gcggtaaaaa aagaagacgc ggtcaggatt
    11641 ttgagcggaa aagcccgtga agaagaaccg tccaaaccca cgcccgaaga cattttggaa
    11701 cataatgccg ccggagggga tgcagacgta ccccaagccg cagaaggcgc gcccgaaccg
    11761 gaaatcctgc atcctgacga cggcgagcgt gccgataccg ttaccgtatc acggggcgaa
    11821 gtggaagagg cgcgcgtaca aaaccagcgt gcggaatccg aaattaccaa actttgggga
    11881 ggactcgata ccgacgtgca aaaagagttg gtcggcgaac aacgcaagtg ggcgcaggaa
    11941 aaaatcagca actgccgaca agccgccgcg caggcagacc ggcaggaata cgccgaatac
    12001 ctcaagctgc aatgcgacac gcggatgacg cgcgaacgga tacagtatct tcgcggctat
    12061 tccatcgatt aggggcaaac cgatgaatac cgtcccaaaa agcaggattc ccgtcaaacc
    12121 gctgcccgaa aaaaccacag acgaagccaa agtcgaaaaa tggcggcagc tcggtgcgga
    12181 acacggtttg tcgggcgaat gggcagttgc cgtcagattg ggcgaaaacg gttttaccga
    12241 agaacagatg gaaaatatcg ccaacctgtt cggcagataa agagaaaatt gacggaaatg
    12301 ccgtctgaaa ccctgttatc ggtttcagac ggcattttga ccaatacggt acgcaggcgc
    12361 aaaacagccg gcttttcctg tgttgcctat gctgatgttt caacacgcag gacgatatat
    12421 aaagcgttgc cctatgtgcc gtcctgattc ggaaagggtt acgcccctcc
//
LOCUS       DAWFAN010000045        12240 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWFAN010000045 DAWFAN010000000
VERSION     DAWFAN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.045"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(280..3132)
                     /gene="gcvP"
                     /locus_tag="WGO65_000899"
     CDS             complement(280..3132)
                     /gene="gcvP"
                     /locus_tag="WGO65_000899"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ0327210.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNMINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGINVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3163..3525)
                     /locus_tag="WGO65_000900"
     CDS             complement(3163..3525)
                     /locus_tag="WGO65_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327211.1"
                     /translation="MTDRPNLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILLARCPPL
                     SRLLCYPCPNHPMSKNSA"
     gene            3473..3703
                     /locus_tag="WGO65_000901"
     CDS             3473..3703
                     /locus_tag="WGO65_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0327212.1"
                     /translation="MISACPGFIHSRFGRSVMPSEPADHLFQKTPYRRNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            complement(3696..4337)
                     /locus_tag="WGO65_000902"
     CDS             complement(3696..4337)
                     /locus_tag="WGO65_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ0327213.1"
                     /translation="MPSEKPMNRRIFLLGAGALLLTACGRKSARTHAKIPEGSSVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVLV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4327..4878)
                     /locus_tag="WGO65_000903"
     CDS             complement(4327..4878)
                     /locus_tag="WGO65_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ0327214.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFDEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAV"
     gene            complement(4882..5400)
                     /locus_tag="WGO65_000904"
     CDS             complement(4882..5400)
                     /locus_tag="WGO65_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ0327215.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5400..7061)
                     /gene="pqiB"
                     /locus_tag="WGO65_000905"
     CDS             complement(5400..7061)
                     /gene="pqiB"
                     /locus_tag="WGO65_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ0327216.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7054..8514)
                     /locus_tag="WGO65_000906"
     CDS             complement(7054..8514)
                     /locus_tag="WGO65_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ0327217.1"
                     /translation="MGSAVGGQPLEKIFLPDTNGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGGRADVPRLDRGEAAFCPR
                     CGHKLFRVGRHPFSGPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMVRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            8864..9493
                     /locus_tag="WGO65_000907"
     CDS             8864..9493
                     /locus_tag="WGO65_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ0327218.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGDKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            9668..12139
                     /locus_tag="WGO65_000908"
     CDS             9668..12139
                     /locus_tag="WGO65_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HEZ0327219.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVLAADEAAIETTPVKAEIKAVRVK
                     GKHNASTTVERVKAKTIQEQMIRDTRDLVRYSSDVGVSDDGRRMKGFAMRGVEDNRVG
                     ISIDGVALPSSEENSLYARYGNFNNSRLSIDTELVKGVDITKGADSFESGSGSLGGNV
                     NYRTLDARDIVLPEQNFGALLRSGYASKNREWANTAGFGYAGNVFDAVVLYSRRHGHE
                     MKSGGDVVDFVGFGELDKQDQAERGSARIHPDPSKHNNHSYLVKLGWQPADSHRFGVS
                     VNGQNNSNYTYEKTYSLTSYWREADDVQKRTNINLNHEWTPASSVLSLLRTDLDYQKT
                     RNGAINYKGDMVRTGDWRTGYQYHNGFMADRDERNMDTKYKRITFRLDSRPFSLWAGE
                     HRLSFKTFASRRDFENVNDDLDFNSTGNITDRKHYTIIRPMKTDQIGFSLQDKIAFDD
                     KLSVNAGVRYDYEKVKPQAFKPDTPCLSTCVNDGNPSARSFNTWNGVLGLDYLFNDTW
                     RAAYQINTGHRVPTASEMYFTYTSPYGNWIANPSLKAERSLNQTLSLRGKNDKGLLDF
                     SLYQTRYRNFLFEEEKTYQVPNEYYNEHSCYYNPKYCNPTLTEIGQQMVNIDKARVRG
                     VEFKGELNLHQVMSVPEGFKVSGALGYSKGKLSGERGSLLSIQPLKLVLGFDYESPDE
                     RWGIFSRVSYMGRKKAKDAQVIEQKSYCTSYKLDPWTGKETDDCAVALQYRDDKIDFR
                     WLNKAATVVDVFGYYKPVKRMTLRAGVYNLFNRKYHTWDSLRGINPRSTINSLSVSNT
                     KSANQGLERYYAPGRNYAVSLEWKF"
BASE COUNT         2946 a         3006 c         3323 g         2965 t
ORIGIN      
        1 gaggcaacgc tgtactggtt tttgttaatc cactatattt gtttgtccgt gtggaaatga
       61 catcaaaggg tgtgaatagg cttttcatct ttgaaatctg tttgcacgat cggatgaata
      121 aaaagtagga tgataaaaaa gctgatgtat ggatactggt ttaactcatt tgttttaaag
      181 ttatagtcga gatacgtgta ttggtgcaac cagtctgccg ttgacgaaaa tgccgtctga
      241 aaatgtttca gacggcattt gtcttcgggg tttcaacggt cagtcttcat aattttccat
      301 cggtgggcag ctgcacacga gattgcggtc gccgtacacg tcgtccacac ggttgacgct
      361 cggccagaat ttgtgttcgc ggacgaatgg caacggaaag acggcttctt cacgggagta
      421 cggatgcgcc cattcgccgg ttacatcgga tgcggtatgc ggagcgttga ccagcggatt
      481 gtcctctttc ggccattcgc cgcgcccgac tttcagcact tcctgtttga tttgtttcag
      541 ggcggcgatg aagcggtcga gttcggcttt gctttcgctc tcggtcggct cgatcatcag
      601 cgtaccggca acggggaagg aaaccgtcgg ggcgtggaag ccgtagtcca tcaggcgttt
      661 ggcgatgtcg gtttcagtga tgccgctttc ggctttgagc ggacgcaaat cgacgataca
      721 ttcatgcgcg acgcggccgt ttttgcctgt atacagaatc ggatagtctt cgctcaggcg
      781 tttggcaacg tagttggcgt tgagcaatgc ccagcgtgtc gcctgttcca tgccttgttt
      841 gcccatcatg gtcaggtaca tccaagtaat cggcagtatg gacgcagaac cgaaggccgc
      901 agcagcaacg gcggtttggt cggcactggc gctgtgggtg tcggtcaaag cgtgtcccgg
      961 tgcgaacgga gccagatggg ctttcaagcc aatcggaccc atgcccggac cgccgccgcc
     1021 gtgagggata cagaaggttt tgtgcaggtt catgtgcaac acgtccgcgc cgacttcggc
     1081 aggctgcatg atgccgattt gggcgttgag gttggcgccg tccatgtaaa cctgtccgcc
     1141 gttttcatgg acgatgcggc agatgtcgcg gatgccttct tcgtacacgc cgtgggtgga
     1201 cggataggta atcatgatgg cggacaaagc gtcgcggtgt tgctcggctt tggctttcaa
     1261 atcgtcgatg ttgacgttgc cgtgttcgtc ggtatcgacg acgacgactt tcaaaccgag
     1321 catggcggcg gtggcggggt tggtgccgtg ggcggatttg ggaatcagac agatgttgcg
     1381 ctgcgcttcg ccttgcgctt cctgatagcg gcggatggca agcataccgc tgtattcgcc
     1441 ctgcgcgccg gagttgggct ggaaggaaat tgcatcaaaa ccggtgatgg atttcaggct
     1501 gttttccata tcggcgagca attcgcggta gccggcggtt tgcgcttcag gagcgtaagg
     1561 atggatgtcg gagaactccg cccaagtaat cggcaacatt tccgcagtcg cgttgagttt
     1621 catggtgcag ctgcccaatg aaatcatgct gcggttcatc gccaagtcgc ggtcttcgag
     1681 ttttttcagg tagcgcagca tttcgtgttc ggtgtggtaa cggttgaaca cgggatgttg
     1741 cagaatgtcg tcttgacgca gcagttcggc gttcagacgg cctttgacat catcggcaaa
     1801 tgcgaacgta tccttgccgg taaacgcgcg gtacaaatca accaaatctt cgcatgccga
     1861 tgtttcgtgg aatgcagccg caacttgagt atcgttaacg cggcgcaggt tatagcccga
     1921 ttccaaagca gcggcaaaca cttggtctgc tttctctttg ttgccgaaat cgacggtaac
     1981 ggtatcgaag aagactttgt gaaccacatt aatgccgtct gaaaccagcg catcggcaaa
     2041 agcagaagcc agcgcgtgaa tgcggttggc gatgcgtttc acgccttcag ggccgtggta
     2101 aacggcgtac atacccgcca agttcgccag caatgcctgc gcggtacaaa tattggatgt
     2161 cgctttttcg cggcggatgt gttgctcgcg ggtggacaac gccatgcgca aggcaggttt
     2221 gcccgatgcg tctttggata cgccgatgat gcggcccggg gcggaacgtt tgaactcgtc
     2281 tttaaacgcg aaataagcag catgcggacc gccgaagccc atcggtacgc cgaagcgttg
     2341 ggtgttgccc aaagcaatat ccgcgcccaa ttcggcaggc gatttcagca aaaccaagct
     2401 cataatgtcg gcggaaacgg ccacgattgt gcctttggct ttcagacggc cgataacgtc
     2461 ctgcaagtct tgcacatcgc cgtctttgcc gacgtattgg aacagcgcgc cgaagtattc
     2521 gccctcgtcg gcttgagcaa aatcgccgac caccaattca aagccgaaat atttggcgcg
     2581 ggttttcatc acgtccaaag tttgcggata cacgcgcgca tccacgaaga aacgctcgga
     2641 tttcactttg cccacgcggt gcgccatcgc catcgcttcg gcggcggcag tcgcttcgtc
     2701 cagcaaagac gcgcctgcca cggggaagcc ggtcaaatcg atacacactt gttggaagtt
     2761 caacaacgct tccaaacgcc cctgggcgat ttccgcctga tacggcgtgt aggcggtgta
     2821 ccaacccgga ttttccaata cgttacgcaa aatcacgttc ggcacgcggg tcgggtaata
     2881 gcctaaaccg atataggatt tgttgatcat gtttttcgac gcaatgcctt tcaatttcgc
     2941 caatgcgtcc gcttcggtca gggcttcggg caaatcgagt tcggacggca tacggatgct
     3001 ttgcggcacg gtattgccga caaaatcgtc catgcttttc tcgccgacag cggcaagcaa
     3061 cgccgcttcg tcgccgaaac tcaaatgccg cgcggcaaat tcgtcggggt tgaacagttc
     3121 ggacagtttc atttctactc cttcgggttt gtagggctgc catcaggcag aatttttaga
     3181 catcggatga ttcgggcaag ggtagcacaa aagacgggat aatggcggac aacgcgccag
     3241 cagaatgcag caggtcagga caagcagcgc cacaagcggc agcgataagg gaaagccgcc
     3301 gcccaaaacc atgaaacctg caggcaccag ccaagccaat ggatacgccg gaagcggaaa
     3361 aacaatattg agcagaacca aaatcacgac aaacagcggc agacccgcca tcaggttggc
     3421 acgcctgtat gttgcgtacg ggtcggatgc ttcttcaggt tcgcaatatt ccatgatttc
     3481 agcctgtccg ggctttatcc acagcaggtt cggacggtcg gtcatgccgt ctgaaccggc
     3541 agaccatctt tttcaaaaaa cgccataccg ccgcaacttc atgcggctgt atggcgtagg
     3601 ttttgcactt cagacggcat atgcgtcccg aagcagtttg gtgttccagt cggttcatga
     3661 ttatataaac aacttgggtg cggataaacc tttgtttatc taaacccctg ttttctcaaa
     3721 aattgattca aatcttcggc aaatttccga tagcctttgc cgttggcgtg gatttggtcg
     3781 gatttcagat tattatcgcc caaaatttcc gcccacgcgc cgccgaacag cggaatgccg
     3841 tattcctcgg acaaatcctc atacagcgga tgatcgctca aatgtccgaa caacgcgccc
     3901 agtgtgatgt gcggcacgcc gacgaggacg gcggggatgt tttccttctg cacggtttcg
     3961 atgattttcg cgatattggc gcgggtctgc tccttgggaa ctttgcgcag aaagtcgttg
     4021 ccgcctatgc cgacaagcac aagcttgggt ttgcgtgcca acagcgcggg caggcgcgac
     4081 agggcttggg cggatgtatc gcccgatacg ccgccgttga caatattcca acccgtcagt
     4141 ttttgcagtt gcgcggggta ggattcgcca gggtttgcgc cgtagccgaa ggtaagcgaa
     4201 tcgcccaagg caagtacgga gcttccttcg ggaattttgg cgtgggttcg ggcggatttt
     4261 ctgccgcagg cggtaagcag caacgcgcct gcgccgagga ggaagattct tctgttcatt
     4321 ggtttttcag acggcatcga gccatttcgg gtggtatttc agggtttcgc ggtaaaccgg
     4381 acagctttcg gcgtgcgcgc ctttgaggta gccggtactc atcagaaatt cgccgacgat
     4441 ttcgccgccg acgaatttga aatgtttttt aaagagtttg acccattcgt ctttgcttcg
     4501 cggatggtgc gcgtcaagcc agttcttgaa cgagccgtat tcttgttgca acgcttggat
     4561 ttgccgtgca ttgaaaatgg cggcatcgat tttcaggcgg ttgcggacga tgcccgcatc
     4621 ggcaagcagc cgctcaatat cggcttcgtc aaatgcggca accgtatcga tgtcgaaccc
     4681 ttcaaatgct gtctgaaacg cctgccgctt cttcagcatc agcgtccagc tcaatcctgc
     4741 ctgattgatt tccaacacca gccgttcaaa caattcattg tcgtcctcaa tcggaaaacc
     4801 gtattgcgtg tcgtggtaat gtttgttcgg gttgtcggtg ttttcgggaa gtgaggcggc
     4861 aaattcgcaa tagttcacgg tttactcgac catctgttgc gccgcctgtt tcagtccctg
     4921 ttcgagtgcg gcggtcatcg cggcgtagcc gtcaccctgc tgttcggttt cgatatggaa
     4981 gggtctgttc gtaccgtcgg gtaggacggc gtagccgctg atgagggttt tgcccgtgta
     5041 gctgccttgg aatgcgtcaa tatagaccgt ccatttttcg gtactgccgc tgcgtgaggc
     5101 aggaacaaag atgcgtgtgc tgtccaaacg gttgaatgca ttgctcaacg ccgcttcgag
     5161 catatcgtcc aaggtgtccg cccaaacgtg gttttgtgcg gtgttgaggc ggtaggggtc
     5221 ggtttgatag accagtccgc cgcgtttgag cggttcggca aggcggactt cgacggcggt
     5281 ttcgccgcct tgcgttgcag ggcggatgta gcggctgtcg ggcaacacga aatattgtgt
     5341 gctttgcaca gtaccgcagg cggcaagcgt cagggcggcg gcgatgggga agaggcgcat
     5401 tatcggcttc ctttcgggat agggtctttg ctgctgctgt tgaaaatcag cgcgttgggt
     5461 ttttctttca aagtgttaat gacgggttga acgtctttta aggttttgtc caaactttgc
     5521 agcgtatttt gtacgtcgcc gtagataggc gattgaggcg atacgccttg cagggttgtg
     5581 cgcaactctt tcagggtttg gttcagttcg ttcggaatgt tttgtgtctg cggtttgccg
     5641 accagtttgt cgatggagct tagggcggca ttggcagatt tgagtgtgga tttgagctcg
     5701 gcaagcgaac cgttcaattc ggcaaccgtc ttatctaaag gcagtttgtc gaacttgtcc
     5761 agcaaatccg ccaatttgac ctgcaaatcg tccaaaccgc cgccctgggt cgcgataacg
     5821 gtatcgcctg cataaacggt atgcggtcgc agcttgggcg aagacgaagg ctgatcgttc
     5881 aactcaatca ttttgctgcc ggtcagcagg ttgttgctgg agatggtggc ggtcaggcct
     5941 ttgtttaagg ccgtctgaaa ttgttgtttc caatgttctt tgctttgctc gtcggcattg
     6001 atttccaaac gggaaggctc gatgcggatg cgtacgggaa tccaaccgtt ttcaaacaga
     6061 tgcaggctgt cgttgcggtc gaaataaggg acatcggaaa ccacgccgac attcagccct
     6121 ttgtactcga cgggcgaacc gacggtcagg ccgcgcacgg attgtttgaa aaacgcggtg
     6181 tagtacagcg agcggtcgtc aggcaggttg gcgacttcgc tgcggctgtc gtaaagcgtg
     6241 aagctgtctt cgcttttgac gtttttactg tttttggttt tcggcgaatc aaatgaaatc
     6301 gcgcccgaca gcagggcagg cagaggggcg gaattgagtt tgatgccgct gcctgtggtt
     6361 tcgatattga tgccgctttc cagccagaaa cggctggcgg aatgaatcag tttgtcgttg
     6421 gggctttgga tgaagatggt gtaatgcacg ctttggtcgg acgggtcgaa atgcgcgctt
     6481 tcgacttgcc cgaccataaa gttttcatac aaaacagggc tgttgacgtt gaggatgcgg
     6541 tcgtttttac caatcaaatt caagcgcagc ccgctttgcc cgatggcggt aacgggcgga
     6601 atgtcctgca cttggaacac gtcttttgcc tcgtcgcttt tgccgggtgt aaaagcgatg
     6661 tacgaacccg aaagcagcgt acccaaaccg gttacgccgc tttggtcgat acgcggcttg
     6721 accacccaaa actgggtatc gctgcggatg aggccggata cgtccgcatt gagttgggcg
     6781 gttacttcca cgcctttttg gtcgtcgcgc agtttgattc gggtaacgcg tccgacatcg
     6841 atgctcaata ccttaatgac cgtattgttg acctcaatgc cttccgcgct gtccatcaag
     6901 agcgtaacca caggccccct gttgcggatt tccttaaccc aaagccagcc gccggcaatc
     6961 agcgcaatca gcgggaccag ccagacggcg gagagcaagg tgttgttttt gcggacgcgt
     7021 gcttgggcgt gtccgtttgg aggagggctg ttgtcagtca tatttttccg tttcattgaa
     7081 agcaatgccg tctgaagcgc gtttgtccca aagcaggcgc gggtcgaaat aataggcgga
     7141 cagcatcgtc agaatcacga ccaggcagaa atagactgcc gcactgcccg gaatgacgcg
     7201 cgcggcataa gtgtggaacg aacacatcaa aataataatc acaaaaatat caatcatcga
     7261 ccagcggccg actgcttcgg tgatgcggta gaggtgcgac aatttctttg cgcccgttgg
     7321 caaagcgaag cgggcggacg caatcaaaac cgacattgcc gcaatcttca gtaccggcac
     7381 caaaatactc gcgctgaaaa taaccgccgc aatcagcctg tcgccctcgt cccacatata
     7441 agcgatgccg ttaaggatgg tattgacctc tgtggcggca ggattggacg aaatcataat
     7501 cggcaggata ttggcaggga aatacaaaat aaccgctgcc gtcagaaacg ccgacgaaat
     7561 actcagactt ttcggccgtc ggcggtacag ttccgcgccg cacaccccgc agggggattc
     7621 ggcactgtcg cggaaataca ggcagcggct gcaacaggtt ttgccttccg atgccgtctg
     7681 aaccgcatta tcccccgtca gccgcccgat ttgaaaatac acccaatgct ggggaaccga
     7741 taccgaagtc cgaatcagca taaccgacag cgcgaacatc agataaaacg ccgacccgaa
     7801 gcgaacctct gccacagacg agagcttgat atacgccacc aaagtggaaa caaaaaacac
     7861 atccaccatc atcgcctgcc tcaagcgcac catcacacgc gttgccaaac gcagcgcagg
     7921 atacgcctgt ttccgtatca gcgcggcata gacatacagg cacagcagca gaaacagaac
     7981 cggcgcgccg aaagtcagca caaacatcac ttcggccaaa aaaccataat cctgaaacac
     8041 catcaggcgt accatttcgg gtagcgaaag gacggatgcc gcacccggta tcccgacctc
     8101 gatatacgtc ataccgtaag caaacgccat caaaatcagc gaagccgccg cataggcggg
     8161 cgggccggaa aaaggatgcc tgcccaccct gaagagtttg tgtccgcaac ggggacagaa
     8221 cgccgcctct cccctgtcca accggggcac atccgcgcgg ccgccgcatt ccggacaatc
     8281 taccgtatgt ggcggcaggg cttcgtcgcg gcagttgccg cgaaggctgt gggggatatt
     8341 ttcggcaaac ggcttcatcg ggaaagtggc aaatataagt ggcggcatta taaaggaaaa
     8401 aaggcgatac acccaatcag gatgcaaaac cggcaggttc ggcagtatga acaaaccatt
     8461 ggtgtccggc aaaaatattt tttcgagcgg ttgtccgccg actgccgaac ccatttgata
     8521 aaatcccgat tctgtaaaga atagtaaaat acctgcttaa aaacatcacg caaatgatat
     8581 tttttccaaa aatatgtgat aataaatttc gtttatgatt ctttattatt tttatagtgg
     8641 attaacaaaa atcaggacaa ggcggcgaag ccgcagacag tacaaatagt acggaaccga
     8701 ctcacttggt gcttcagcac cttagagaat cggtctcttt gagctaaggc gaggcaacgc
     8761 cgtactggtt tttgttaatc cactatataa tccactatat atgcgggcaa gtgcggattt
     8821 tgcccgcccc gcatccttat cccttaccga caaaggaaaa cttatgagtg aaaccgaaaa
     8881 tcaagccctg acgtttgcca aacgtctgaa ggcggatacc acggcggttc acgacagcgt
     8941 ggataatctc gttatgtctg tccaaccgtt tgtcagcaaa gaaaactaca tcaaattttt
     9001 gaaactccaa tccgttttcc acaaggctgt cgaccacatc tataaagatg ccgaattaaa
     9061 caaagccatt cccgagctgg aatacatggc gcgatacgat gccgtaacgc aagaccttgc
     9121 agatttgggt gacaaacctt acgaatacgg caaaccgctg ccgcatgaaa ccggcaacaa
     9181 agcaatcggc tggctttatt gcgccgaagg atccaatttg ggcgcggcat ttttgttcaa
     9241 acacgcccaa aaactcgatt acaacggcga acacggcgcg cgccacctcg caccccatcc
     9301 cgacgggcgc ggcaaacact ggcgcgcctt cgtcgagcat ctgaacgctt tgaacctgac
     9361 tcccgaagcc gaagcggaag ccatccaagg cgcgcgcgaa gcctttgcat tctacaaagt
     9421 cgtgttgcgc gaaaccttcg gcttggcagc cgatgccgaa gcaccggaag gaatgatgcc
     9481 gcacaggcac taaaaaataa tcgaaccaaa taaacaaggt ctcggcatag ctgtttgcag
     9541 ggacctttaa ttacacggcg cggctttgtt tacatggatt actgtcttat taaatattaa
     9601 tgattatcat aaaatctatt attcgctaac cgatggatga acaatccata catcttgagt
     9661 tgataatatg aaaccattac aaatgctccc tatcgccgcg ctggtcggca gtattttcgg
     9721 caatccggtc ttggcagcgg atgaagctgc aattgaaacc acacccgtta aggcagagat
     9781 aaaagcagtg cgcgttaaag gtaagcacaa tgcatctacc actgtggaac gagtaaaggc
     9841 taaaaccatt caagagcaaa tgattcggga tacacgcgat ttggtgcgtt attcatcaga
     9901 tgtcggtgta tccgatgatg gtcgccgtat gaaaggcttt gccatgcgcg gggtggaaga
     9961 caaccgtgtc ggtatcagta tcgacggcgt ggcattgccc agttcggaag aaaattcact
    10021 ttatgcacgt tacggcaact tcaataattc ccgtttaagc atcgataccg aattggttaa
    10081 gggtgtggat attacgaaag gtgcggattc atttgaaagc ggcagcggct ctttgggtgg
    10141 taatgtgaac taccgcacgc ttgatgcacg ggatattgtt ttgccggaac aaaatttcgg
    10201 tgcacttctt cgcagtggtt atgcaagtaa aaacagagag tgggcgaata ccgcaggttt
    10261 cggctatgcg ggcaatgttt ttgatgccgt ggttttgtat tcccgccgac acgggcatga
    10321 aatgaaaagc gggggggatg tcgttgattt tgtagggttt ggtgaattgg ataagcagga
    10381 tcaggcagag cgcggatcgg cacgcattca tccagatccg tccaaacaca ataaccacag
    10441 ttatttggtg aaattaggct ggcaaccggc cgactctcac cgcttcggtg tttctgttaa
    10501 tggacaaaat aacagcaatt acacttatga aaaaacttac tccctgacaa gttattggcg
    10561 ggaggcagac gatgtgcaga aacgcaccaa tattaacctg aaccacgagt ggacacctgc
    10621 atcttccgtc ctttctttgt tacgtacgga tttggactat cagaaaaccc gcaatggggc
    10681 gataaactat aaaggtgata tggtccgtac cggagactgg cgcacaggtt atcagtatca
    10741 caacggcttt atggctgata gagacgagcg gaatatggat accaaataca agcgcattac
    10801 cttccgtttg gatagccggc ctttctcact gtgggcaggt gaacacagat tatcttttaa
    10861 gacttttgcc agtcgtcgcg actttgaaaa tgtaaatgat gatttagatt ttaattcaac
    10921 aggcaatata actgaccgta aacattacac cattatacgt ccgatgaaga cggatcaaat
    10981 cggtttttca ttgcaggata aaatcgcttt tgacgacaag ttatctgtaa atgcaggcgt
    11041 gcgctacgac tatgagaagg taaaaccgca ggctttcaag ccagatacgc cttgtctgag
    11101 tacctgtgtt aatgacggta atccgtccgc aagaagtttt aatacatgga acggcgtttt
    11161 aggtttggac tacctcttta acgatacttg gcgtgcagct taccaaatca atacggggca
    11221 tcgcgtgccg acagcatcgg aaatgtattt tacttatacc agcccttacg gtaattggat
    11281 tgctaaccca tcgttgaaag cagagcgcag tctaaaccaa actctgtcac tgcgtggaaa
    11341 gaatgacaaa ggattgctgg attttagcct gtatcagacc cgttaccgta atttcttatt
    11401 tgaggaagaa aaaacgtatc aggtgccgaa tgagtattat aatgaacata gttgctatta
    11461 taatcctaaa tattgtaacc ctacgcttac cgaaatcggc caacaaatgg tcaatattga
    11521 taaagccagg gtgaggggag ttgaatttaa aggtgagttg aacctacatc aagtgatgtc
    11581 tgtgccggaa ggttttaaag tatccggcgc attgggttat agtaaaggca aattgtccgg
    11641 tgaacgcggc agtttgttgt ccatccagcc gctgaagttg gtgcttggat tcgattatga
    11701 gtctcctgat gagcgttggg gtattttctc ccgtgtcagc tatatgggca ggaaaaaagc
    11761 caaagatgcc caagtaattg aacaaaagag ctattgcaca agctataaac tcgatccttg
    11821 gacaggtaag gaaacagatg attgtgctgt tgccttgcag tatcgtgacg ataaaatcga
    11881 cttccgttgg ttgaataaag cggcaactgt tgtggatgta ttcggctact acaaaccggt
    11941 aaaacgtatg actttacggg cgggcgttta taatctgttt aaccggaaat accatacttg
    12001 ggacagcttg cgcggcatca atccgagatc gactattaac tctttaagcg tatcgaatac
    12061 taaatcagca aatcagggtc tggaacgcta ctacgccccc ggccgcaact atgccgtatc
    12121 gctggaatgg aagttttaat ctggtattat tgaattaatc gccttgttga aaattaaagc
    12181 cgtccgaatt gtgttcaaga attcattcgg acggcttttt accgtaaatc tgtgtgatgg
//
LOCUS       DAWFAN010000046        12195 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWFAN010000046 DAWFAN010000000
VERSION     DAWFAN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12195
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.046"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            207..944
                     /locus_tag="WGO65_000909"
     CDS             207..944
                     /locus_tag="WGO65_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0327220.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            947..1633
                     /locus_tag="WGO65_000910"
     CDS             947..1633
                     /locus_tag="WGO65_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0327221.1"
                     /translation="MAGLTFEQTVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1792..2655
                     /locus_tag="WGO65_000911"
     CDS             1792..2655
                     /locus_tag="WGO65_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327222.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2717..3286)
                     /locus_tag="WGO65_000912"
     CDS             complement(2717..3286)
                     /locus_tag="WGO65_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ0327223.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3444..4304
                     /locus_tag="WGO65_000913"
     CDS             3444..4304
                     /locus_tag="WGO65_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ0327224.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTLETFDHI
                     LKQLGIDYRP"
     gene            4439..4777
                     /locus_tag="WGO65_000914"
     CDS             4439..4777
                     /locus_tag="WGO65_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327225.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5145..5498
                     /locus_tag="WGO65_000915"
     CDS             5145..5498
                     /locus_tag="WGO65_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ0327226.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5488..6354
                     /gene="atpB"
                     /locus_tag="WGO65_000916"
     CDS             5488..6354
                     /gene="atpB"
                     /locus_tag="WGO65_000916"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ0327227.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6411..6647
                     /gene="atpE"
                     /locus_tag="WGO65_000917"
     CDS             6411..6647
                     /gene="atpE"
                     /locus_tag="WGO65_000917"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ0327228.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6718..7188
                     /locus_tag="WGO65_000918"
     CDS             6718..7188
                     /locus_tag="WGO65_000918"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ0327229.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7193..7726
                     /locus_tag="WGO65_000919"
     CDS             7193..7726
                     /locus_tag="WGO65_000919"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ0327230.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKM
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7737..9284
                     /locus_tag="WGO65_000920"
     CDS             7737..9284
                     /locus_tag="WGO65_000920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ0327231.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9309..10184
                     /gene="atpG"
                     /locus_tag="WGO65_000921"
     CDS             9309..10184
                     /gene="atpG"
                     /locus_tag="WGO65_000921"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ0327232.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10222..11619
                     /gene="atpD"
                     /locus_tag="WGO65_000922"
     CDS             10222..11619
                     /gene="atpD"
                     /locus_tag="WGO65_000922"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ0327233.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVNNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11630..12052
                     /locus_tag="WGO65_000923"
     CDS             11630..12052
                     /locus_tag="WGO65_000923"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ0327234.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         3001 a         3115 c         3146 g         2933 t
ORIGIN      
        1 ttttgttaat ccactatatt gtccgaaatg gttgcccgct tcctgcctta ttgacaaaaa
       61 aacgctttcc cgataatatc ctacgaaaat taacctgccg atttgacaca gcttgcgggc
      121 ataacagcta aagcgttccg acaatttcag ctttatcttc cgcgcccgtt gtgtccgaca
      181 tcgggctttg ttgtatggga aagacaatga ttattttgga caaggtttcc aagcattacc
      241 aaacgcgcga caagacccgt tttgccgctg tcgagccgac cagcctcgaa atccgcgacg
      301 gtgaaatctt cgggctgatg ggttattcgg gtgcaggcaa atccaccctg ttgcgcctga
      361 ttaacctgtt ggaacgtccc gacagcggca aggtcaacgt ctgcggacaa gagctgaccg
      421 cgctcgatgc cgccgcattg cgtcaggctc ggcagaatat cggcatggtg tttcagcagt
      481 tcaacctgtt gaacaaccgc accgtcgccg gcaacgtcgc cttcccattg gaaatcgccg
      541 gatggccgtc tgaaaaaatc aaagcgcgcg ttgccgaatg tctcgaaatc gtcggactga
      601 ccgaacgcgc cggccactat cccgcccagc tttcaggcgg gcagaaacag cgtgtcggca
      661 tcgcccgcgc actcgcgccc aaaccccaag tcatcctcgc cgacgaaccc acttccgccc
      721 tcgaccccgc caccacgcgc agcgtcttgg aatgtttgga agacatcaac aaacgattca
      781 acgtaaccat cgtcatcgta acccacgaaa tgagcgttat cagacgactg tgcgaccgcg
      841 ccgccctgtt ggataaaggc aaagtcgtcg aaatcgtcga agtacgcggc aaccaaatcc
      901 acgcccgatc cgacatcggg cgcgaactga ttcgggagga ctgaatatgg caggcttaac
      961 atttgaacaa accgtttcca acatcatcgg tatgaaaggc gaaatcgtcc gcgcattggg
     1021 cgaaaccttc gtaatggtcg gtttgtccac cacattcgcc gtcgttttcg gcacgttgct
     1081 gggcgtgttg ctcttcgtaa cttccagccg ccaactgcat tacaacaagc cggtgaactt
     1141 cctgctcgac aacctcgtca acctcatgcg cgccttcccc ttcgtcattt tgatgattgc
     1201 gatgataccc gccacacgcg ccatcgtcgg cagcaccatc ggcccggttg ccgcctcgct
     1261 ggtgttgagc gtgtcgggat tgttttattt tgcccgactg gtggaacaaa acctgcgcga
     1321 agtccccaaa ggcgtaattg aagccgccgc cgcgatgggt gcgccgccgg ttgccatcgt
     1381 ccgcaaagtc ctcttaaacg aagcgcgcgc gggcatggtt tccagcatta ccgtgcttgc
     1441 catcgggctt ttgtcataca gcgcggcggc agggatgata ggcggcggcg gcttgggcga
     1501 cctcgccatc cgctacggct actaccgcta ccaaaccgaa gtcatcgtct tcatcgtcgc
     1561 cctcctcgtg ctgctggtca tcctgattca aagcaccggc aacgcattgg cgcggaaatt
     1621 ggataaacgt taaacccgaa tgccgtctga acgccaaaac ccccaccgct atccgaaaaa
     1681 tgctataaaa tccccctgtt cgcggcaaat gccgtctgaa cgccgaatct ggacggcagg
     1741 actccctgcc cgtcattttt gtttgaaact gccacaacat caggagaaaa tatgaaaacc
     1801 ttcttcaaaa ccctttccgc cgccgcactc gcgctcatcc tcgccgcctg cggcggtcaa
     1861 aaagacagcg cgcccgccgc atccgcttct gccgccgccg acaacggcgc ggagaaaaaa
     1921 gaaatcgtct tcggcacgac cgtcggcgac ttcggcgata tggtcaaaga acacatccaa
     1981 cccgagctgg agaaaaaagg ctacaccgtc aaactggtcg agtttaccga ctatgtgcgc
     2041 ccgaatctgg cattggctga gggcgagttg gacatcaacg tcttccaaca caaaccctat
     2101 cttgacgact tcaaaaaaga acacaatctg gacatcaccg aagtcttcca agtgccgacc
     2161 gcgcctttgg gactgtaccc gggcaagctg aaatcgctgg aagaagtcaa agacggcagc
     2221 accgtatccg cgcccaacga cccgtccaac ttcgcccgcg tcttggtgat gctcgacgaa
     2281 ctgggttgga tcaaactcaa agacggcatc aatccgctga ccgcatccaa agcggacatt
     2341 gccgaaaacc tgaaaaacat caaaatcgtc gagcttgaag ccgcgcaact gccgcgtagc
     2401 cgcgccgacg tggattttgc cgtcgtcaac ggcaactacg ccataagcag cggcatgaag
     2461 ctgaccgaag ccctgttcca agaaccgagc tttgcctatg tcaactggtc tgccgtcaaa
     2521 accgccgaca aagacagcca atggcttaaa gacgtaaccg aggcctataa ctccgacgcg
     2581 ttcaaagcct acgcgcacaa acgcttcgag ggctacaaat cccctgccgc atggaatgaa
     2641 ggcgcagcca aataaggcag tcgtataaaa tgatgccgtc tgaaccgtat ccgtgttcag
     2701 acggcatttt tgtcctttaa tccgccattc cctgccattc cgccgaatcc ggcgtatcga
     2761 ttccgaacag cgacaaagcg tgtgcaacac tgtgcgccac tatgtcgtcc gccgtctgcg
     2821 gtttgcggta catcgcagga acagggggaa acaccacgcc gcccatttcc gttacccgct
     2881 tcatattgtc caaatgggca aggttcagcg gcgtttcgcg caccatcagc accagccgcc
     2941 gcctttcctt caaaaccaca tccgccgcac gcgtcaagag gttgtcgccg aagccgtgcg
     3001 cgaccgaggc aagcgtccgc atcgaacagg gggcgaccag catcccgtcc gttttaaacg
     3061 taccgctggc aatgcacgcc ccgatattgc cgatcggatg cacgaagtcc gccaaggcat
     3121 atacctcatc tctcgcataa gccgtttccg aagcgcgcgc catctccgca cctttcgata
     3181 ccacaaggtg cgtttcgata tcttgcgcgc gtaaaagttc caaagccttc acgccgtatt
     3241 ggaaaccgct cgccccactg atgccgatta tcaaacgccg taccatcatc cgcctttccc
     3301 ataaaaccgc ctgcaacggc aaaccggcta ttatagtgaa aaaacagaaa tccgataaat
     3361 acggatacaa attgtcggca acacccaata tccgataaaa tatccgattt aacatcctat
     3421 ctgaataggc acgggagggc ggtatggcaa aagtaaaagg cggattgggg cgcggtttgg
     3481 attcgctgct cgccaacggc gcagacaaca gcagcggcga ccgattgacc acggttgcgg
     3541 ttaaagatat ccggcccggc cgctatcagg cgcgtgttca aatcgatgac gaagccttgc
     3601 aggagctggc ggattcgatt aaagcacagg gagtgataca gcccgtcatc gtgcgcgaac
     3661 acggactgtc ccgatacgaa ctgattgcgg gggagcggcg ttggcgtgcc gcacagattg
     3721 ccggcctgac cgaaatcccc gccgttatca aaaccatcag cgacgaaacc gcgttggcaa
     3781 tgggtttgat cgaaaacctc cagcgcgaaa acctcaaccc catcgaagaa gcacaaggct
     3841 tgaaacgcct tgccgacgag ttcggactga cccacgaaac catcgcccaa gccgtcggta
     3901 aaagccgaag cgcgatttcc aacagcctgc gccttttaag cctgcccgaa cccgtgcagg
     3961 aaatgcttta ccaacgccgc ctcgaaatgg ggcacgcccg cgcactgctg accctgcccg
     4021 tcgtcgaaca gctcgaattg gcgcaaaagg ccgtcaaaaa cggctggtcg gtgcgcgaag
     4081 tcgaacgccg cagccaagcc gcccttcaaa acaaacgtcc cgagcccaaa aagactgccg
     4141 ccgccgacat cggccgcctg aatgatttgc tgactgaaaa actgggtgtc aacgccgaaa
     4201 tcaaaaccgc caaccataaa aaaggcaaaa tcatcctgca tttcgacacg ctcgaaacct
     4261 tcgaccatat cttgaagcag ttgggcatag attaccgacc ttaattttgc gggatatacc
     4321 gtctgaaata tagagaatag ctttccagat tttaagtggg aaatataatt ctattgacat
     4381 ttttctgctt cacgtaagaa tcgttttcct gttttcattt ttaattttcg aggaaattat
     4441 gaacacacgc atcatcgttt cggctgcgtt cgttgcgttg gcattagcag gttgcggctc
     4501 aatcaataat gtaaccgttt ccgaccagaa acttcaggaa cgtgccgcgt ttgccttggg
     4561 cgtcagccca aatgccgtaa aaatcagcaa ccgcaacaat gaaggcatac gcatcaactt
     4621 taccgcaact gtgggtaagc gcgtgagcca atgctatgtt accagtgtaa tcagcacaat
     4681 cggcgttacc acttccgatg caatttgttt gggaggcgga acgcacaaag gcaaaagtca
     4741 atgcaatgct ttgcttaaag cggcaggccg ttgctaatcc tttattcgga aaaggtcgtc
     4801 tgaaaatatt ttcagacgac cttttattta ttgagcaaat tccccaaact gccgacaatc
     4861 cgaccgataa actgctacaa tttcgcgttg attttttatt atttccacgt aaagcaaaca
     4921 aatcacgtaa gaacattaaa aatgtaaaga aattgcggac aaactcctta atatcctgta
     4981 attatatgta atgtgtgtta gaatgacacc tgtcgccgtt attcaggcgg cgagcgcggc
     5041 ggattgggat acgttgtttc acaaagatta actgtcgctt gacgtaataa ggcttctaaa
     5101 ttatattagc cgcgccccaa atccgaggcg gaaacattcc ctttatgaag cagattatca
     5161 tcctacaatc cgccgtattg tccatatgcg ccgcagttgc ctttgctgta tggggttttg
     5221 ccggattcct ttcggcggtc ggtggcggtt tgtcctacct gcttcccacg tttgttgcag
     5281 ttttactttt aaaacttttc aggggaaacc ccttcctgca aagcaggatg ttcgtcttcg
     5341 gagagatttt aaaagtagtg ctgtcgctgt tgtccatgct cgccgtattt gctgtatggc
     5401 atcaatcgtt ggtgttcgcc ccgtttctga tggggctgct cggtgtcagc catttggttt
     5461 ttttagtatt gttgagagtg aaagattatg gcaggtgaaa ccattaccgc tgccgactac
     5521 atcaagcacc acttgcagag cttgaccagt ttgtcggatg ttactcaggg tcagggactg
     5581 aaaaacattg ctgatttttc gtttattaac cttgatgccg tcttttttgc cgtcctgttg
     5641 ggcgtaatcg gcagcttcct gttgtggcgc ggtgcgaaaa aagcaacggc aggcgttccc
     5701 ggacgtttcc aggctgccgt ggaaatcttg tttgagtttg tggacgatat gtgtaagagc
     5761 atcattcaca acgaaaagtc tagaaaagcc gtcgcgccgt tgggtctgac gctgtttgtc
     5821 tggattttcc tgatgaacgc gatggatatg ctgccggtcg atttgctgcc gatggtatgg
     5881 cagggcatta ccggcaacca tcacgccctg ttgcgcgtcg taccgactgc cgatttgaac
     5941 accactttgg cactcgctgt cggcgtgttg ctgatttgta tctattacaa catcaaaatc
     6001 aaaggattgg gtggctggtt tcacgagttg ttcagcgcgc cgttcggtgc aaaactcgca
     6061 cctgcgaact ttctgttgaa tttggtcgag tttctttcta aaaccgtatc ccacggtatg
     6121 cggttgttcg gcaatatgta tgcgggcgag ttggtattct tgctgatagc cttgcttggc
     6181 ggtgcttggg cggcttccgg cagcgtcgaa gtcatggatc cgattctgtt tgtattccac
     6241 attattgccg gtttggcatg ggcgattttc catattttgg tgattaccct gcaggcgttt
     6301 attttcatgg cgttggcgtt cgtttatatc ggacaggcac atgatgcgca ctgattttcc
     6361 taagtagttt gtttttgtag tttaaccttt gtttctttaa ggagtttaaa atgggtttga
     6421 ttgctatcgc atgtggtttg atcgttgcat tgggtgcatt gggtgcctct atcggtatcg
     6481 caatggtcgg ttctaaatat ttggagtctt ctgcccgtca gcccgaactg atcggtccgc
     6541 tgcaaaccaa actattcctg attgccggtt tgattgatgc cgcattcttg attggtgtcg
     6601 ccattgcact gctgttcgcc ttcgttaacc cgtttgcagg tgcataatca ggacggattc
     6661 gtccgttccg tacaggcgca gcctgtttga ttaaccccag tacgaaggtt aagtaacgtg
     6721 aatatcaatg caacattatt cgctcaaatc atcgtctttt tcggtttggt atggtttacc
     6781 atgaaattcg tgtggccgcc gattgcaaaa gctttggatg agcgtgccgc aaaagtcgcc
     6841 gaaggcttgg ctgccgccga gcgtggtaaa agcgattttg agcaggctga aaaaaaggtt
     6901 gcagaactct tggcagaagg gcgtaatcag gtttccgaaa tggttgccaa cgccgaaaaa
     6961 cgtgccgcca aaattgtcga agaagccaaa gaacaggctt cttccgaggc ggcgcgcatt
     7021 gcagctcagg caaaggccga tgtggagcag gaattgttcc gcgcacgcga atccctgcgc
     7081 gagcaggttg ccgtgttggc tgtcaaaggt gccgaatcta ttttgcgcag cgaagtcgat
     7141 gcttccaaac acgcaaaact gctcgatacc ctgaaacagg agctgtaatc ttatggcaga
     7201 gttcgcaacg attgccagac cttatgcaaa ggcattgttc ggtctggctc aggaaaaaaa
     7261 ccaaattgag tcttggttgg gcggactgga aaaacttgcg gcggttgttc aggaagggaa
     7321 gatggcttca ttgattgacc gtcctgaaac gaatgcttca gaaaaagcag atatcctcat
     7381 cgatttggtc ggtttgaaag acaaggagtt gaaaaacttt gttatcgtct tggccgggca
     7441 gaaacgtttg tcgatattgc cggaagtata tgctcaatat caagacttga ccttatcatt
     7501 caaccatatc aaatctgccg tcatttacag tgcctatccg ttgaccgaca aacaggtcgg
     7561 cgagttggcg caaatgctga ataagcgttt cgacagcgag ctgaaaatct ctgtcgaaat
     7621 cgaaccggag ctgattggcg gcataaaagt tgaagtgggt gatcaggttt tggatttgtc
     7681 tgtacaaggc aaattgagtg ccttgtacac gactatgacg aattaggaga gttttcatgc
     7741 agcttaatcc tgctgaaatt agcgatttga ttaaagccaa gatcgaaaat ctgtctgtaa
     7801 atgccgaagt gcgtactcgt ggtacggtga tttcggtaac tgacggtatc gttcgcatcc
     7861 atggtttgtc agatgcaatg caaggtgaga tgctcgaatt cccgggtaac actttcggct
     7921 tggcgatgaa cctggagcgc gactccgtcg gcgccgtagt gttgggcgag tacgaacata
     7981 ttaaagaagg cgacacggtt acctgtaccg gtcgtatctt ggaagtgccg gtcggacgcg
     8041 aactggtcgg acgcgtcgtt gatgcattgg gtcggcctat cgacggcaaa ggcccgatta
     8101 atacaacttt gaccgcccct atcgaaaaaa tcgcaccggg cgtgattgcg cgcaaatcgg
     8161 ttgaccagcc gatgcaaacc ggtctgaagg cgattgactc tatggttcct gtcggtcgcg
     8221 gtcagcgtga gttgattatt ggcgaccgtc aaaccggtaa aacggccgta gcattggatg
     8281 ccatcgtcaa ccaaaaaggt acgggtgtta tctgtatcta tgtcgctatc ggtcaaaaag
     8341 catcttctat tgccaacgtg gtccgcaaat tggaagagca tggcgcgatg gagcatacca
     8401 tcgtggttgc tgcaactgca tctgaagcgg cggcattgca atatatcgca ccttactccg
     8461 gttgtacgat gggtgaattc ttccgcgatc gtggcgaaga tgccttgatt gtttatgacg
     8521 atttgtccaa acaggctgtg gcttaccgtc aaatttccct gcttttgcgc cgtccgcccg
     8581 gccgcgaagc ctatcccggc gatgtgttct acctgcactc ccgtctgttg gaacgtgcag
     8641 cacgtgtcaa tgaacacgaa gtggagaaat tgaccaacgg cgaagtaaaa ggcaaaaccg
     8701 gttctctgac cgcgttgccg attatcgaaa cccaagcggg cgacgtatct gctttcgttc
     8761 cgactaacgt catttcgatt accgacggtc agattttctt ggaaaccgac ctcttcaacg
     8821 ccggtatccg tcctgcaatc aatgccggta tttccgtatc ccgcgtaggc ggtgctgcac
     8881 aaaccaaagt gattaaaaag ctgggtggcg gtatccgttt ggcgttggcg caatatcgtg
     8941 aattggcggc gttctcgcaa tttgcatccg atttggatga agctacgcgc aaacaattgg
     9001 agcatggcga agtcgtaacc gaactgatga aacagaaaca gttcagtacg ttgaatacgg
     9061 ctgaaatggc tttgaccctt tgggcaatca acaacggttc gtattctgat gttccggttg
     9121 ccaaagctct ggcttttgaa tctgaatttt tgagctttgt ccgtacccaa catccggaag
     9181 ttttggaagc cgtcaatgct tcaggtgcaa tgtccgacga gagcgagaaa acgcttgaag
     9241 cagccatgaa atccttcaaa tcttcttacg cctatcaggc ataaggcttg aagtgaaagg
     9301 agtctgaaat ggcagtagga aaagagattc tcaccaaaat ccgcagtgtt cagaataccc
     9361 aaaagatcac taaagcgatg cagatggtgt caacctctaa aatgcggaag actcaggaac
     9421 ggatgcgttt ggcgcgtccg tatgccgaaa aagtgcgtat ggtgatgagc catcttgcgc
     9481 aaaccaatac cgatcatggt attccgttac tggaatctca tcgggaaatc agacgtgtcg
     9541 gttttatttt gattacgtct gataagggtt tgtgtggcgg tttgaacgcc aacgtgctga
     9601 aaaagttttt ggcacaagtt caagagtatc ggaatcaagg tattgaagag gaaatcgtat
     9661 gccttggcag taaaggtctg atggcgtgtc agagcattgg tctgaatgtg gttgccagtg
     9721 ccgtaaattt gggcgatacc ccaaaaatgg aaatgctgct cggacctttg acagaactct
     9781 tccaacggta tgagaaacat gaaattgaca gaatccatct ggtgtattcg ggttttgtca
     9841 ataccatgcg tcaagaaccg agaatggaag tattgctgcc tatcggtgag aacgtgattg
     9901 gcgattcagc tcccaaatca ccgttcagct gggaataccg ctacgaaccg actgcacttg
     9961 cagtgttgga atatctggtt cgccgctatt tagagtctgt ggtttatcag gcgttgagcg
    10021 acaatatggc atccgaacag gcagcccgca tggttgccat gaaagctgca acagacaatg
    10081 caggcaatgc catcaaagag ttgcgtttgg tatataacaa atcgcgtcaa gctgcgatta
    10141 ccacggaatt gtcagaaatt gtagcaggtg cggcggctgt ctgatgccgt ctgaatcctg
    10201 tacggaaatt aggatacgat aatgagccaa ggcaaaatcg tacaaattat cggtgcggtt
    10261 gttgacgtgg aatttccacg cgacatgatt ccgcgcgttt acgacgcttt gaaattagac
    10321 gaaaacggtc tgactttgga agtccaacag cttttgggcg acggcgtagt ccgtaccatt
    10381 gcgatgggca gctcggacgg tttgaaacgc ggcatgactg tgaacaatac cggtgcgccc
    10441 attactgtgc cggtaggtaa aggtacgttg ggacgcattg tcgatgtatt gggaacgcct
    10501 gttgacgaag caggtccgat cgataccgac aagagccgtg ccatccacca agccgcccct
    10561 aagtttgacg aactgtcttc cacaaccgaa ttgctcgaaa cgggcattaa agtgattgac
    10621 ttgctgtgtc cgtttgccaa aggcggtaaa gtaggtctgt tcggcggtgc cggtgtgggc
    10681 aaaaccgtga acatgatgga attgatcaac aacatcgcca aagcgcacag cggcttgtcc
    10741 gtgttcgcag gcgtgggtga gcgtacccgc gaaggtaacg acttctacca cgagatgaaa
    10801 gattccaacg tattggataa agtggcaatg gtttacggtc agatgaacga acctccgggc
    10861 aaccgtttgc gcgtcgcatt gaccggtttg accatggcgg aatacttccg tgacgaaaaa
    10921 gacgaaaacg gcaaaggccg cgacgtattg ttcttcgtgg acaacatcta ccgttacact
    10981 ctggccggta ccgaagtatc cgcattgttg ggccgtatgc cgtctgcagt gggctaccaa
    11041 ccgacattgg cagaagaaat gggtcgtttg caggagcgta ttacctctac ccaaaccggt
    11101 tctattactt ccatccaagc cgtatatgta cctgcggacg atttgaccga cccgtctccg
    11161 gcaacaacgt tcgcccactt ggacgcaacc gtcgtattga gccgcgatat tgcctctttg
    11221 ggtatttacc cggcagtcga tccgcttgat tccacttcgc gccaattgga tccgatggtc
    11281 ttgggtcaag aacactacga cgtggcgcgc ggcgtacagt ccaccctgca gaaatacaaa
    11341 gaattgcgcg acatcatcgc catcttgggt atggacgaat tgtctgatga agacaaactg
    11401 accgtaatgc gtgcgcgtaa aatccaacgc ttcctgtccc aaccgttcca cgttgccgaa
    11461 gtgtttacag gttctccggg taaatatgtc gccctgcgcg ataccattgc cggcttcaaa
    11521 gccatcttga acggcgaata cgatcatctg cccgagcagg cattctatat ggtcggcagc
    11581 atcgaagaag cggttgagaa agcgaaaacc ttaaactgag gaggtcggca tgagcatcat
    11641 gcaagttgag gtggtcagta gcgagcaaaa catctattcg ggcgaagcca gctttgtagt
    11701 ggttccgacc gttcaaggtg agcttggtat ttacccgcga cacgagccga ttatgagttt
    11761 ggtgcggccg ggggctttgc gtttgaccgt tccgggcgag gataaagagg ttttggttgc
    11821 tgtttccggc ggtgttttgg aagtacagcc tgataaagta actgtcttgg cggatgttgc
    11881 cgtccgcagt gcggagatgg atcaggcacg tgcggaagag gcgaaaaaag ccgcagaagc
    11941 gggcatttcc caagctaaag acgataaggc tttggcggaa gcacataaag cattggctgc
    12001 tgcgattgca cagctcaaaa ctttggacta tatccgttcg cacaagaaat aaacttgttt
    12061 ggtttggaaa agcacgaccg tttggtcgtg ctttttattt tccggattta aattaaagac
    12121 tgtgtttgga tatggcgttg gcaggcatag cctgctttaa ggtattcaat agggcagcct
    12181 gccgcctaac cgaaa
//
LOCUS       DAWFAN010000047        12148 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWFAN010000047 DAWFAN010000000
VERSION     DAWFAN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.047"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            141..1538
                     /gene="aspA"
                     /locus_tag="WGO65_000924"
     CDS             141..1538
                     /gene="aspA"
                     /locus_tag="WGO65_000924"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ0327235.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1610..2485)
                     /locus_tag="WGO65_000925"
     CDS             complement(1610..2485)
                     /locus_tag="WGO65_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0327236.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2487..3209)
                     /locus_tag="WGO65_000926"
     CDS             complement(2487..3209)
                     /locus_tag="WGO65_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ0327237.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQQAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSRHAYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYLLGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNDGRAVAPEKWI"
     gene            complement(3232..3711)
                     /gene="ybaK"
                     /locus_tag="WGO65_000927"
     CDS             complement(3232..3711)
                     /gene="ybaK"
                     /locus_tag="WGO65_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ0327238.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFSRLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3786..4148
                     /locus_tag="WGO65_000928"
     CDS             3786..4148
                     /locus_tag="WGO65_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0327239.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWCVEIKITAVKRDAATA"
     gene            4183..5046
                     /locus_tag="WGO65_000929"
     CDS             4183..5046
                     /locus_tag="WGO65_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ0327240.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPSLAFTAECGSDGICSVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLAADGEEAGIVLDSVKDSESFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5144..6103
                     /gene="ttcA"
                     /locus_tag="WGO65_000930"
     CDS             5144..6103
                     /gene="ttcA"
                     /locus_tag="WGO65_000930"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ0327241.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(6237..6791)
                     /locus_tag="WGO65_000931"
     CDS             complement(6237..6791)
                     /locus_tag="WGO65_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0327242.1"
                     /translation="MKPKFKTVLTALFLAASLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGFTEDLVQKATDKAVANESGNGLKN
                     TAGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNIPVQPVKIRRVVAE"
     gene            complement(6856..7782)
                     /locus_tag="WGO65_000932"
     CDS             complement(6856..7782)
                     /locus_tag="WGO65_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0327243.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMEQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKTKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCPPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKFTDGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            complement(7770..8246)
                     /locus_tag="WGO65_000933"
     CDS             complement(7770..8246)
                     /locus_tag="WGO65_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ0327244.1"
                     /translation="MKTNFKQKIIDQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGAVAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            complement(8317..8772)
                     /locus_tag="WGO65_000934"
     CDS             complement(8317..8772)
                     /locus_tag="WGO65_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ0327245.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKICQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETYRLADAGCRALLEKISK"
     gene            complement(8897..10012)
                     /locus_tag="WGO65_000935"
     CDS             complement(8897..10012)
                     /locus_tag="WGO65_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ0327246.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            complement(10126..11223)
                     /locus_tag="WGO65_000936"
     CDS             complement(10126..11223)
                     /locus_tag="WGO65_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ0327247.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            complement(11305..>12148)
                     /locus_tag="WGO65_000937"
     CDS             complement(11305..>12148)
                     /locus_tag="WGO65_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ0327248.1"
                     /translation="AFNDPIYVNGFIEWANRHRLAYIGDADLHASFVSWMAEHTRERI
                     LALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESVAVEVIESLNFRPARG
                     ETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLTFDRMKINSQLLLQTIL
                     GRFSVSSDNAGKQFFEDHKTYVPARFTDYAAAFVEHGAEAFVRPANRYNESTPSFGYG
                     HLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEVYVEEILADLADRHFL
                     VSAD"
BASE COUNT         2951 a         3345 c         2973 g         2879 t
ORIGIN      
        1 cccgaaaaca acgtttcccg ttttcagacg gcatacgata tggacaattt catccggccg
       61 gtatagtgtt cgtcctgcgg cggcatcttt ccgatggcgc attttttcaa tattaattat
      121 ccgacttaca aaggtaaaac atgactgtcc gtatcgaaca cgatttattg ggcgaccgcg
      181 agattcccgc cgaagtgtat tggggcatcc acactctgcg cgccattgaa aactttaaaa
      241 tctccacaca aaaaatttcc gacgtgccgc agtttgtccg cagtatggtg atggtgaaaa
      301 aagcaaccgc gcaggcaaac ggcgaattgg gtgcagtaaa acccgaaatc gccgccgcca
      361 ttgaaaaggc ttgcgacgaa gttctgctga acaaccgctg cctcgaccaa ttcccctccg
      421 acgtgtatca gggcggtgcg ggaacttcgg ttaatatgaa caccaacgaa gtcatcgcca
      481 acctcgcatt ggaagccttg ggatacgaaa aaggccgcta cgacatcgtc aatccgatgg
      541 atcacgtcaa cgccagccaa tccaccaacg atgcctaccc taccggcttc cgccttgccg
      601 tgtattacag catcggcgaa ttgctcgaca aactgactgt cctgaaaaac gcctttgccg
      661 ccaaagccga agaatttaaa gacgttttga aaatgggccg cacccagctt caagatgccg
      721 tgccgatgac tgccggccaa gaattccaat ctttccaagt gttgttggaa gaagaaatcc
      781 tcaacctcga ccgcacccgc caactgctgt tggaagtcaa tttgggcgca acggcaatcg
      841 gcacgggcgt gaacacgccc aaaggctacg ccgaactggt ggtcaaaaaa ctctccgaag
      901 tcagcggctt gccttgcaaa ctgaccgaaa acctgattga ggcgacctcc gactgcggcg
      961 catatgtgat ggtacacggc gcattgaagc gcacggcggt caaactctcc aaaatctgca
     1021 acgacttgcg cctgctctct tccggcccgc gtgccggctt gaaagaaatc aacctgcccg
     1081 aattgcaggc cggttcttcc ataatgcccg ccaaagtcaa tcccgtgatt cccgaagtgg
     1141 tcaaccaagt ctgcttcaaa gtcatcggca acgacacgac gattaccttc gccgccgaag
     1201 ccgggcaact gcaattaaac gtgatggagc cggtcatcgc ccaatgtatg tttgaaacca
     1261 tttccctctt gggcaatgcc gcagtcaacc tgtccgacaa atgcgtcaaa ggcattacgg
     1321 tcaaccgcga aatctgcgaa cgttacgttt tcaactccat cggtttggtg acttatctga
     1381 atccgtatat cggacaccac aacggcgatt tggtcggcaa aatctgcgcc caaaccggca
     1441 aaggcgtgcg cgaggttgtc ttggaacgcg ggctgttgag cgaagaagaa ctcaaccgca
     1501 tcctctctcc cgagaacctg atgaaccccc atctgtaacg ttgcagacaa atgccgtctg
     1561 aagcagggat gctgcttcag acggcatttt gttatatcaa tcagccggat tatatgccga
     1621 acagtgcctt gacctccgcc caataaaaca ggcaggcgat ggaacacagt gccaacacga
     1681 ttcccgccgt attgattgtg ttgatttttt ccttaaaaaa cagtgcgccc gaaagcgtcc
     1741 ccaaaacaat cacgccgata ttcataccgg caaaaaccag cgtgggattg tccttcatca
     1801 tttggtgcgc ggtgatgtag gttacgatgt tcatgaagtt caaaccgccc aagagtatgc
     1861 cgccgaccac actctcaacg cgccatctga ccgatttggc aaacaggcag gcaaacatca
     1921 gcacacccgc cagcacaaat gcaaccagca ggttgcccgc aaatgccgtt ccgcttttgg
     1981 caagctgttt gaacaggata tcgataatgc cgtaacctgc ccacacgccc agcagcaatg
     2041 ccgcctgccg ccacgcgctt cctgattttt tgccaccgct gtgtttccaa agcaggcaga
     2101 acagtgcggc aaatgcgagg cacagcccga ttagtctgcc ttcgctgagt ttttcgccaa
     2161 acagcgtcaa ggcggcaaca atcggcaaaa acagcgacaa acgctgcgcc gcgtcggatt
     2221 tgacgatacc ggcggattct acggatttgc ccataatgac aaatacggac ggcagcagca
     2281 cgcctaatgc ggcaaacagc ggccacgtcg gcaaaaatgc gccgatattg ccgacatccg
     2341 gcttcaatac cagcaaagtc agtatgaccg cgaccacata attgacggcg accgcctgcg
     2401 cgatgtcgat ttttttcttc cttgccattt tcagcaaaat ggaaaccgac acgctgaaca
     2461 cgatgctgat taacaggtaa gccatactat atccatttct cgggtgcaac cgccctgccg
     2521 tcgttgaagc ggataaagcc ggcgatgatg cggtagaaat accagacgtt cgttgcaata
     2581 ataatcagca cgccgatacc caaaagggca gtcagcgcgc ccaaaagata aagccaaaat
     2641 gtgcgccaaa aggttttaat caggtattcg gtatgtgcgg catagacaat gctgtccaaa
     2701 ctgttccgtt tcatataggc gagaatcacg ccgactatgg gcatataggc gaaaatcacg
     2761 ccgactatgg gcatgaacag catgattgca cccaaaccga acaggatgta ggcggcgagg
     2821 acatagttgc gcccgcttcc gccgccggac ggttcgtaag catggcgcga acgtgaggcc
     2881 tgccgttcaa acgcctgtct gaacgcctgt tcgcggcgca tctgttcgcg gtaaaactgc
     2941 tccctgcgcg cctgttcgcg gcggaatgcc tcttcctgcc gcccgcgttc ctcatgccgt
     3001 ctgaaggacg cgtcgtattg catccgtttc gacaggtcgg aaagcgtatc gtaagcctgt
     3061 tggatttctt tgaacttttc ttccgcctgc ggattgcccg gattgcggtc gggatggtat
     3121 ttcatcgcca gcttgcggta ggcgcgtttg atttcgtccg ctcccgcctg cggcgacacg
     3181 cccaatacgg catacaaatc tttgtccata ttgttccttt atactttccc gtcaaaccgc
     3241 cgcctgtatt gttttcgggt tcaaaatatt taaatctccg ggacggatgc cgataatgaa
     3301 cccgcgtttt ccgccgttga tatagatggt ttccaaatcc atcaccgact gttcgacgta
     3361 aatatccaac tttgtccgga tgccgaacgg cgttgtgcct ccgaccagat agcccgtcca
     3421 cttgtttgcc tgtgcgggcg tagcgggttc gatgtgtttc gcacccaaat gccgagccag
     3481 attgcgcgtg gaaatctgtt tgtcgccgtg catcaagaca atcagcccct taccgttttc
     3541 atcttgcaaa acaatggttt taatgaccaa gtgttcgtct ttgccgaaca gtcgggaaaa
     3601 ctgcgccgtg ccgccgtgtt cctcataggc ataggtaaaa ggttcaaatt cgatgccgtt
     3661 ttcacgcaaa acgcgcacgg caggtgttat cggataagtg tgtttactca tttttcgtca
     3721 tgcaaaacat ggaaatcggc caagcgcaat gttaccatat atccccttta tcgaaggaaa
     3781 ccgatatgga tatccgatat ttcggcacaa cgccccgcta ttccgaagcc gtcggcgcaa
     3841 acggcttgat tttcctctcc ggcatggttc ccgaaaacgg tgaaacggct gccgaacaga
     3901 ctgccgacgt gcttgcccaa atcgaccgct ggctggcgga atgcggctcg gacaaagcgc
     3961 acgttttgga tgcggtcatc tatctgcgcg atatgggcga ctatgcggaa atgaacggcg
     4021 tgtgggacgc ttgggttgcc gccggcagaa cgcccgcccg cgcctgcgtg gaagctcggc
     4081 tcgcccgtcc ggagtggtgc gtcgaaatca aaataaccgc cgtgaagaga gacgcggcaa
     4141 ctgcataaac agatttcctt tatatttcag agtacgcaaa tgatgaaaac cttactgccc
     4201 tttttcggcg tggtgcgcgt cagcggcgaa gaccgccaaa ccttcctgca cggacagctt
     4261 tccaacgaca tcaaccacct tcagaccggg caggcgtgtt atgcgactta caacacgccc
     4321 aaaggccgcg tcatcgccaa tatgattgtc gtcaaccgag gcggcgattt gctgctgatt
     4381 atggcgcaag acctgcttga agcaacggtc aaacgtttgc ggatgtttgt gttacgcgcc
     4441 aaagccgttt ttgaaatcct tgaagattac gccgtcggcg cggaattaga agcatccgcc
     4501 gaaccgcttg ccgcgcaaga acccagcctc gcctttaccg ccgaatgcgg ttcagacggc
     4561 atctgttccg tcgtcctgcc acatcggggc attttgcaca tcgcccccaa aaacgccttg
     4621 ccgccttatg atgccgccgc cgaaaacgcg tggcggctgc acgaaatccg cagcggttac
     4681 ccgtggatat gcgcggctac caaagaaact gccgttgccc aaatgctcaa ccagcacatc
     4741 atcggcggcg tacacttcaa aaaaggctgc tatcccggtc aggaaatcat tgcccgtgcc
     4801 cagtatcgcg gtcaggtcaa acgcggcttg gcggtattgt cgggaaattc ggcagccgaa
     4861 gcaggcatcc tgctggcggc agacggcgaa gaggccggca tcgtgctcga cagcgttaaa
     4921 gattcggaaa gctttaccgc ccttgccgtg attaaattct ccgccgcaca aaaagaactg
     4981 accgccccaa acggcagtat cttcaaagcg gttcatccct tctttaaaac cgaaaatgca
     5041 gaataatccc tctaatgccg tctgaacctt tttcagacgg cattttaaaa ccgcccctgt
     5101 tttcagttac aatgccgcat ccccaatttt tcaaaccacc gccatgtcca aaaaaaccaa
     5161 acaagaactg gaaaacaaca aactcagcaa acgcctgcgc cacgccgtcg gcgacgcgat
     5221 taacgatttc aacatgatcg agccgggtga caaaatcatg gtctgcctct ccggcggcaa
     5281 agacagctac gccctgttag acatcctgcg ccggcttcaa gccagcgcgc cgattgattt
     5341 cgaactggtc gccgtcaatc tcgaccaaaa gcagccgggc ttccccgaag aagtattgcc
     5401 gacctatctc gaaagcatcg gcgttcccta caaaatcgtt gaagaagaca cctactccac
     5461 cgtcaaacgc gtattagacg aaggcaaaac gacttgttcg ctgtgcagcc gcctgcgccg
     5521 cggcatcctc taccgcactg caaaagaatt gggctgcacc aaaatcgcct tgggacacca
     5581 ccgcgacgac atcctcgcca ccttgttttt aaatatgttt tacggcggca aactcaaagc
     5641 catgccgccc aagctggtga gcgacaacgg cgaacacatc gtcatccgtc cgctggcgta
     5701 tgtaaaagaa aaagacttaa tcaaatacgc cgaattaaaa caattcccca tcatcccgtg
     5761 caacctctgc ggctcgcaac ccaacctgca acgccaagtc atcggcgata tgctgcgcga
     5821 ttgggacaaa cgcttccccg gccgcatcga atcgatgttc tccgccctgc aaaacgtcgt
     5881 cccttcccac cttgccgata ccgaactctt cgactttgtc ggattggaac gcggtcaaac
     5941 cctgaaacac ggcggcgatt tggcgtttga cagcgaaaaa atgcccgaac gtttttcaga
     6001 cggcagcgaa gaagacgaaa gcgaaatcaa aatcgagccg caaaaagccg aacgcaaagt
     6061 catcaacatt ctggcgaaca agccgaaaac ctgcggcgcg taaacctcca ccgtcaaaaa
     6121 tgccgtctga agcccgtttg gcgtttcaga cggcatttta aaatcccgaa gtagacactt
     6181 ttcaacccca aacgcctgct ttttccgttt cagctttctg aaggtttccc gttatattac
     6241 tcggcaacaa cgcggcgaat tttgacgggc tgtacgggta tgttttgata aaagccgcgc
     6301 gtggcggttt tgacgcgggc gattttggaa acggtgttca tgccgctttc gaccctgccg
     6361 aaaacggtat agccgtattg tccgtttttg tagttgagtg aatcattgtc ggccagattg
     6421 ataaagaatt ggctggtggc ggaatccgga tcggccgtcc gcgccatggc gatggtaccg
     6481 gcagtgtttt tcaagccgtt gccggattcg ttggcaacgg ccttatcggt tgccttttgt
     6541 accaagtctt cggtaaatcc gccgccctgg ataacgaaac cgtcgataac gcggtgaaaa
     6601 acggtgtcgt cgtaaaagcc ttttcgagca tagcgcacga aattagcaac ggttttgggg
     6661 gctttggact cgtccaaaac caaacggata ttgcccatgt cggtttccat caaaacatgg
     6721 gttgccgcca tagacggcag ggaagccgcc aaaaacagcg cggttaaaac ggttttgaat
     6781 ttgggtttca tcccgtcctc ctcagacctt cagacggcat tttcatttca tatgccgtct
     6841 gaaagcgcgt gaacactatt ccaatgcgtc tttaagtttt tgttcgatta aatccgcatc
     6901 aaacgatttg gcaatcaatt caaaacggga atcacgccgc caagacactt ggttggcgcc
     6961 ccattgcccg tccacccagt tgagccatac ccatgtaccg agcacttgga acacgccttt
     7021 ggcgcgaaca agaccgtctg tgaatttggg caagtcgttg aagaagttgg tcagcttttc
     7081 gccgtcgaaa tcccgtccgg cggggaatgt gaaaccttgc gactggaatc ccatggtgtt
     7141 gtccggcagg gctttgaggc ggtagcgtga tttttcgatg acgggaatat cgagccattg
     7201 gatgtcgagt tgtgcgtttt ggacttcgac cactttggct ttgggcggga acagttttgc
     7261 cgctttgtcg tgaaattcgg caagctgttc gggggggcat aaatcggttt tgctggcgac
     7321 caatacgtcg caaataccga tttggtcttt atataaggct tgttgcgcgt aatcgggatt
     7381 aatgaattgg cgcggatcga cgacggtaaa gactgcgccg atttccaaaa ggctgtccag
     7441 cggtttggtt ttcagttcat caataacact ggcggcgtgc gccagtccgc ttgcctcaat
     7501 catcaggcgg tcgggcttgg cgtcgcgcag cattttctgt acggttacgc ccatttgcgg
     7561 accggcggtg caacacaaac agccgccggc gatttctgcc acggggatgc cgttgtcgct
     7621 caataccgcg ccgtcaatgc cgatttcgcc gaactcgttg acgatgatga cccatttctc
     7681 gttcgggtct ttctgctcca tcaggctttt aagcgcggtg gttttgcctg ttcccagaaa
     7741 acctgaaatc aggtggactt tggttttttt catttctgac attttttaca gattccagtt
     7801 aaaacaacgt gttcttcttt cagcgcgaag ccgctttcgg caacgcctgc gcgcaatgcc
     7861 gcccactcgt ggctcagggt ttgctcgtcc gccgtgccgc attcggtgca gaccaaaata
     7921 aacgcgctgt ggtgcgcttc ggcttcttcg tggtcgtggc aatggtcgtt gcactcgtgc
     7981 tgcgcgtggc tgcacaaaat atagccgttg accgccgcca ctttgtgcaa aacgccctgc
     8041 tccgcccaaa aatcaagggc gcggtaggcg gtcggcggcg caaccgcgcc ctcgctttgc
     8101 tgctgcatct gcgacaagac gttgtaggct ttaatcacgc cgctttgctg caagacaata
     8161 tctaatacct gctcgcgcaa agcggttacc tgcaagcctt cgctgcgtgc ctgatcgatg
     8221 attttttgct tgaaatttgt tttcataaat tccctgttta tgccgtctga acgaccgata
     8281 cggcaggagg cggttttata tttgtattca attactttat ttggaaatct tttccaacaa
     8341 tgcccgacag cccgcatccg caagcctgta tgtttcttca aaatcgcccg tgtaccacgg
     8401 atcggggaca tggtcgtaac cgctttcggg tatcaggtcg gtcagcttga atattttttc
     8461 cggccgcctg ccgaaggttc tttccaattc ggacaaattc ttgccgtcca tcgcgatgat
     8521 gcagtcaaac gccgccgcat cgctttggca gattttgcgg ctggtaaagc ctgaagcatc
     8581 gataccgtgt tttttcaata tctttgccgt ctcgcggtgc atatcttcgc cgtcgtgcca
     8641 gcccgatgtc cccgcgctgt ccgcttcaag gggaatgccc gcttcggcgg cgcggcggcg
     8701 caaaatgtat tccgccatcg gcgaacggca gatgttgccg aggcagacaa aaaggatttt
     8761 cggttttttc atatcccctc cctgttccgg cgcgatgccg tctgaagcgg aaaccctttc
     8821 agacggcatc ggtcggttaa tcgtaaccgc ataaggcaga accagccaaa acatccatct
     8881 ggaagaaatg gtttggctaa atcttaggca tatttaataa gtgtccaata ttagaagccg
     8941 tatgctccaa atagagactg gcatttttca aactatcttc taaaggttca cttttctcca
     9001 aaatagaaaa ggcagcttgg atattttcaa atggcaggga aggcaaatct tcaacgagac
     9061 tgccacaaat agcgacaaca ggaactccga caggggttct ttttgctaca ccaataggcg
     9121 ctttccctgc taaactttga cgatctagtc ttccttcacc aatgataacc aagtcaacat
     9181 ctgacacttt cttatcaaag tcaatcaagt ccaggcaggt gtcaattcca gatacgatac
     9241 ttgcctgagc aaaggcgcac aaaccaccag cgatgcctcc accagctcct gctcctttaa
     9301 gttttaatgt tgcaggggag actttttcat aaaaatcttg tattgcctga tctacggcct
     9361 caaacatagt agaatccaac cctttttgct tgccaaacgt ataggtcgca ccttggtgtc
     9421 cacataaggg actcacaaca tctgctaaaa tacgaatgtg aacatcttca ggaatttcat
     9481 agcgattttc tgttgaaaca gaagctaggt ttagtaagga ttgaccgcaa acgggtaagg
     9541 tatttccatc ctcatcataa aattgataac ctaaaccagc agcaatccca atacctccat
     9601 cattactggc cgtaccgcca acgccaatat agatttcttt aatttcttga ctaatgaggt
     9661 ggcgaatcaa ttctccaata ccacgagttt ggatttgtaa tggatttcgt ttctctagcg
     9721 ggatttttcc aagaccaacc aaatcagcaa cttcgaatag ggctagttgt tctttttgaa
     9781 aatagcgcat gacttctttt tgtccaaaag gtcctgtcac ttgaagacat ttttcttcta
     9841 ggtcaagaga atgtcggata gcatctacag tgccttctcc cccatcaccg acaggacaga
     9901 ggagacattc cacatctgct atcgattgtt ggaagcctct ttttattgct tcagctacct
     9961 gttgagctgt caagctttcc ttaaacgaat ccggtgcaat tacaatcttc atatttataa
    10021 ttcatccttt cgtttcactc aaggcacaac acagaatgaa aaagtgttgt gctctttatt
    10081 ttgatttatt atataaatga gaaagcctat cactactaca aatcactatg cgctgaaaaa
    10141 cggattgtgc ccttcccgtt tcaatgcttc cgcatagctc gggatgcttt cctgttcgcc
    10201 caagggattg tgcaacaggt aaacgtgttc cacgcggcac tccgccatca ggtcgaggaa
    10261 attttgctgg atgacggcga tgttgtcggt aaaggaaagc tgccacgaaa acacctgcgg
    10321 cacgacttca aacgcgccgt cggtcgcatt aaacccgaat aaaacctgtc cgcccgcctt
    10381 tgccgcagcg accacgccga cgcgcggact ctgcatacgg acggcgcgta tcgcgtttgc
    10441 cgcaaacaca tccatcgcca tacgctggcg cgtgtggctg ccgtctgaaa gcgcgcgtac
    10501 cggagaatcc ggggaaagtg gattggcaaa cagcgtaaca ccttccgatg caagattttc
    10561 ctgccatacc tgcattaagg gcaggcctgc ctgaagaagg ttctgaccca atgccgtctg
    10621 aaccttgccg ctgccgaaac ccagcgcgta aacgacgtgg acggactgcc cttcgggcaa
    10681 cagcaaaggg cgcggggcga aacggcgtaa gtgttgcgcc gccgcttcgg tattttgttt
    10741 ggcacgccac cactcatccg gcgcgaccgc actcaaatcg gacgaatgca caagataagg
    10801 cagccattgt gtctcttgcg tcaacgcgcg caggtgcgga tagttttgca ggcaggcaaa
    10861 caatgcctcc gtcggcagct tcatctcgat tgcccgttct ttcttgcagc cggacaccag
    10921 cacgaccggc agggcgaacc actgcacttc gccttctttc tcgcaatcga gtaccgcgtt
    10981 cacactggaa agcagcgcgg cataagttcc ggcatcgggc gacatcgtca acgcgaggga
    11041 aaggttgata tagtggtttt gctcaagcat ccccctgatt tcggtttgaa gttggccgga
    11101 cgagagtttg cgcgaagcct gggaagaatt atgcgccaac tggtaggcat tgagcagcag
    11161 gtggttctta atcggatttt ggggatacgg gcgcgtatcg ggcaaggtaa atgtctggtt
    11221 catatgtgtc gtgtaccgct tttcgtagtg tgtgtttttc ctgtttgaaa atatatcgga
    11281 cggattgccg tcgggacttg cccgtcaatc cgccgaaacg agaaaatgcc tgtctgccaa
    11341 gtctgccaat atttcttcca catacacttc ggcaggcgga tggaatgtca aaccgtcggg
    11401 cgtggtgctg acgatgttca agttttcggc aaccgtttca atcaatgcct gtttgctcgt
    11461 cggccgcgac aattgccgca taatgtacaa atgcccatat ccgaatgagg gggtgctttc
    11521 gttgtaacgg ttggcaggcc ggacaaacgc ttccgcgccg tgttccacaa aggcggcggc
    11581 ataatctgta aaacgcgccg gcacataggt tttatggtct tcaaaaaatt gtttgcccgc
    11641 attgtctgat gaaacagaaa aacgcccgag aatggtttgc aataaaagct gggaattgat
    11701 tttcatacgg tcgaatgtca aaccgggaaa tcggcgggcg agattttccg caacatcttc
    11761 cgttttaaac ggctctccgg ttttcatcac atcgcgtatg cccgaaagca gggtatcgtt
    11821 ttcatcaaag ttgattgttt cccctcttgc cgggcggaaa ttcaaacttt ctatcacttc
    11881 gacggcaacc gactcgtcac gcctgacagt atccccgact tcctcacggc ataaaagcga
    11941 acggcggaat tggcggtcgg ataaaatatc gctgtaaaat tctttggcaa tataatcgcc
    12001 ccctgccaat gccagaatcc gctcccgcgt atgctccgcc atccaagaaa caaaagacgc
    12061 gtgcaaatcg gcatccccga tatacgccag ccggtggcgg tttgcccatt cgatgaagcc
    12121 gttgacgtaa atcgggtcgt taaacgcc
//
LOCUS       DAWFAN010000048        12123 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWFAN010000048 DAWFAN010000000
VERSION     DAWFAN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12123
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.048"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            14..285
                     /locus_tag="WGO65_000938"
                     /pseudo
     CDS             14..285
                     /locus_tag="WGO65_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            325..1359
                     /locus_tag="WGO65_000939"
     CDS             325..1359
                     /locus_tag="WGO65_000939"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ0327249.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1565..2146
                     /locus_tag="WGO65_000940"
     CDS             1565..2146
                     /locus_tag="WGO65_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ0327250.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2215..2397
                     /locus_tag="WGO65_000941"
     CDS             2215..2397
                     /locus_tag="WGO65_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ0327251.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            2394..3155
                     /gene="kdsB"
                     /locus_tag="WGO65_000942"
     CDS             2394..3155
                     /gene="kdsB"
                     /locus_tag="WGO65_000942"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ0327252.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            3178..3495
                     /locus_tag="WGO65_000943"
                     /pseudo
     CDS             3178..3495
                     /locus_tag="WGO65_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            3600..3797
                     /locus_tag="WGO65_000944"
     CDS             3600..3797
                     /locus_tag="WGO65_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327253.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            3954..4046
                     /locus_tag="WGO65_000945"
     tRNA            3954..4046
                     /locus_tag="WGO65_000945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3988..3990,aa:Ser,seq:gct)
     gene            4349..5134
                     /locus_tag="WGO65_000946"
     CDS             4349..5134
                     /locus_tag="WGO65_000946"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ0327254.1"
                     /translation="MSRIRQAFAALNGEKALIPYITVGDPDIGATLAIMHSMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGMLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            5171..6043
                     /locus_tag="WGO65_000947"
     CDS             5171..6043
                     /locus_tag="WGO65_000947"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ0327255.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRREMKQRISG
                     LITLLCRQDKVSAA"
     gene            complement(6103..6714)
                     /locus_tag="WGO65_000948"
     CDS             complement(6103..6714)
                     /locus_tag="WGO65_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ0327256.1"
                     /translation="MRRTALLLLALTAGTSLAAGFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHDK"
     gene            6823..7047
                     /locus_tag="WGO65_000949"
     CDS             6823..7047
                     /locus_tag="WGO65_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ0327257.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            7048..7194
                     /locus_tag="WGO65_000950"
     CDS             7048..7194
                     /locus_tag="WGO65_000950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327258.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            7410..8444
                     /gene="pyrC"
                     /locus_tag="WGO65_000951"
     CDS             7410..8444
                     /gene="pyrC"
                     /locus_tag="WGO65_000951"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ0327259.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAVLPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQDILFLVHGEVTDPEIDIFDREAAFIERVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(8811..9236)
                     /gene="nusB"
                     /locus_tag="WGO65_000952"
     CDS             complement(8811..9236)
                     /gene="nusB"
                     /locus_tag="WGO65_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ0327260.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAIPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(9314..9790)
                     /locus_tag="WGO65_000953"
     CDS             complement(9314..9790)
                     /locus_tag="WGO65_000953"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ0327261.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRILQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(9838..10161)
                     /locus_tag="WGO65_000954"
     CDS             complement(9838..10161)
                     /locus_tag="WGO65_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ0327262.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            10328..11047
                     /gene="rnc"
                     /locus_tag="WGO65_000955"
     CDS             10328..11047
                     /gene="rnc"
                     /locus_tag="WGO65_000955"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ0327263.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            11094..12017
                     /gene="era"
                     /locus_tag="WGO65_000956"
     CDS             11094..12017
                     /gene="era"
                     /locus_tag="WGO65_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ0327264.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         2792 a         2911 c         3543 g         2877 t
ORIGIN      
        1 aagatactgg aaaatgaaag agatgaaacc tgtccgttat catattggcg atatgcccga
       61 gacttcaaaa caaaccgcct cccgtcatga cgacagggca gtgggtgttg acgatgattg
      121 ttttcatgat tcctttggtc aatttttgtt tgggtgttcg gcagaggcaa cccgaaccgc
      181 gccaatttct gtaaagcgca gttgcttatt tacctgattg gttcgcttat cggtttggtc
      241 ttcgcgttgt ttataggtgg gtctgtatcg ggtacgcatg attaatgccc cgggctgatt
      301 ttgcttcgag gatttgtatc gaatatgccg aattgtttca aatttcatac cgttatcgaa
      361 cggcattggc aaaaacctta tccggttttg tcttttctgc ttaagccgct ctccgggctg
      421 tttgccaaaa ttgcggcaaa acggcggacg gattttttat cgggaaaacg gcaaagcgaa
      481 aagctgcccg tgcctgtggt cgtggtcggc aatattcacg cgggtgggac ggggaaaacg
      541 ccgattgttg ccgcgctggt gtcgggtttg caggaaaagg gcgtcaaggt cggcatcatc
      601 agccggggct acgggcgcaa gagcaaggcg gttcatgtat tgaatgctga gagccgagcg
      661 gaagatgcgg gcgatgagcc tttgctgctg ttccgcaaaa ccggtgcgcc gacggcggtg
      721 ggcagcagcc gtgcagaggc aggcagggcg ttgctggcgg cgcatcccga catcggactg
      781 attgtggcgg acgacggttt gcagcattac gccctgcggc gagatgtgga aatcgcggtg
      841 tttccggcgg cggatacggg gcgcacggat ttggatttac tgcccaacgg cagtttgcgc
      901 gaacctttgt tgcggctgga ttcggtggat gcggtcgtcg tcagcggcgg caaggcggat
      961 gcgctgttta ggccgtctga aaatatgttt cacagccgta tcgaagcggg acggatttac
     1021 cgtttgaaca atccgtccga aatactggac acaggccgtc tgaaaaatca aaccgtcgtc
     1081 gccgtggcag gtattgccaa gccggcgcgt tttttcgatt cgttgcggaa tatgggcatt
     1141 accgtgaagc gaaccgtcgc gctgcccgac cacgccgaca tttcggcggc agatttgccc
     1201 gatgcggacg cggtcattat tacggagaaa gatgcggtca aattttcaga cggcatttgc
     1261 accgataatg tttgggtgtt gcccgtttgt gcgataatcg aacctgattt ggcggcgttt
     1321 gtgttggagc ggttggaaga tgtaccgaag gccgtctgaa agcacggttt gggcggagtg
     1381 attacggatt tgaataagaa cgcctcgcgc cgtcattccc gcgcaggcgg gaatctaagt
     1441 ctcgaatttt caggaatgcc taggaggctt cagaaatccc aaatctccgg atttccactt
     1501 ggacaggaat gagaaaatcg gtcgtatttt ttatctgcat taatcattcg ttaaagtatt
     1561 gaatattaaa ctgaaaacct tgttattgcc cttcgccacg ctggcattgt gcaccaacgc
     1621 ttttgccgcc ccgcccagcg acgcgtcgtt ggcgcgttgg ctggatacgc agaattttga
     1681 ccgggatata gaaaaaaata tgattgaggg ctttaatgcc ggatttaaac cgtatgcgga
     1741 caaagccctt gccgaaatgc cggaagcgaa aaaagatcag gcggcagaag cctttaaccg
     1801 ttatcgtgag aatgttttga aagatttgat tacgcccgaa gtgaaacagg ctgtccgtaa
     1861 tactttattg aagaatgccc gtgagatata cacgcaagaa gaaattgacg gcatgattgc
     1921 cttttacggt tcgcctgtcg gtcagtccgt cgttgccaaa aatccgcgct taatcaagaa
     1981 atcgatgagt gaaatagcag tatcttggac tgcattgtca gggaaaatcg cgcgacatca
     2041 tctgcccgcg tttacggaag agttgcggcg catcatctgc ggcggtaaaa atcccgatgc
     2101 gggctgtaaa caagccggac aggttgggaa aaggcatcag aaataaatga tagccgtctg
     2161 aaatattgaa gagggcatcc gattgattga accatcaaac ccgaaagcaa ccctatggaa
     2221 aaaaaattct tagacatcct cgtctgcccc gttaccaaag gcaggctgga atatcatcag
     2281 gacaaacagg aattgtggag ccgtcaggcg aagcttgcct atccgattaa agacggcatt
     2341 ccctatatgc tggaaaacga agcgcgtccg ttgggcgaag aggaactcga agcatgaccg
     2401 aattcgtcgt attgattccg gcgcggctgg attcgtcgcg cctgcccgga aaagccttgg
     2461 cggacatcca cggcaaaccg atggtcgtgc gcgttgccga acaggcggca aaaagtaaag
     2521 ctgtgcgcgt cgtcgttgcc accgaccatc ccgatattca gacggcctgt caggcgcacg
     2581 gcgtcgaagt cgtcatgact tcaaaccggc acgaaagcgg cacgacgcgc cttgccgaag
     2641 cctctgccgc gctgaagctg ccgccgcatc tggtggttgt caacgtacag ggcgacgagc
     2701 cgctgattgc acctgaactc atcgaccgca ccgccgaagt actcgtcgaa aacaacgtcc
     2761 aaatggcgac cgccgcccac gaattgcacg atttcgacga attgatgaat cccaatgccg
     2821 tcaaagttgt cctcgacaaa aaccgcaacg ccatctactt cagccgcgcc ccgattccct
     2881 atccgcgcga tgcgatgcgt gcaggaaaac gcgaaatgcc gtttgaaacc gccgtcctgc
     2941 gccatatcgg catctacgcc taccgcgtcg gcttcctgca acgctacacc gaaatgagcg
     3001 tttcgccgct ggaaaccatt gaatcgctgg agcaactgcg cgtcctgtgg cacggctacc
     3061 cgattgccgt cgaaaccgcc aaagaagctc ctgccgccgg cgtagatacg caggaggatt
     3121 tggacaggat tcgcgctgta tttcagacta tataaaatag attcaaaggg aaaagatatg
     3181 cagcaacata ttgaaaaatg gcaacacttg agccgggaag aacagaaaat ccttgctgaa
     3241 gtatggggtc tcgtgcaaaa cgacgatcag gaggttcact atgaaatgct caaattgaac
     3301 gcacccgacg aagccagtgg cgaattttgg ttcagaatgg cagaaacact cagcaccctg
     3361 ccgcccaacc gttccctcga ccttagaatg aacggcggca ggctggcgac cgccgtatcc
     3421 atcctttccg tcatgattga agacaatccc gacataccgc agctttgggc gcaaaaaatt
     3481 accgcgctca attatagtgg attaaattta aaccagtacg gcgttgcctc gccttgtcgt
     3541 actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatatt
     3601 tggcacacgg acacaaagcc cgtgccgacg gtttggcaca acagcccgac aaagcggcag
     3661 aagccaacga ggaggaatac ctgaccaaag ccctgtcgca aaacctactg tcaacattgg
     3721 atgccgcgct tgcacgtttt cctgaagacg cgtggtttca ggaaatcaaa caggatgcac
     3781 aaaagcattt tgcttgagga tgtggcagtc aggaatattt ccattcagga agaaaagaag
     3841 tgcctgattg ggtataatca gggtaaatct tattttattt caaaagatta atatttgctt
     3901 tctgtttttc cttgacggta tcggaaaagt tgattatagt tacagcttcc ttaggagtaa
     3961 tggctgagag gctgaaggca cttccctgct aaggaagcat gtggggtcaa cctgcatcga
     4021 gggttcgaat ccctcttact ccgccaagat gagaagacgc tgtgttttac agcgtctttt
     4081 tttacgcgct ttgttgcgga agtgccgagg gctgtctgcc gtgcgccgcc tggatttgca
     4141 atgtgcccgc ccgtgcaaaa tccgatttga ccaacctgaa gggcaggttt cggaagagcg
     4201 gggaattttg ataaacaaaa aactgcacgg cataatcttt cccggttttc attttccgta
     4261 ttcgcctatc cgtaccgcaa atgctatact gggaaaaatt taccgattgt gttttacggc
     4321 atatttgccg ataggatgga agagacaaat gagcagaatc cggcaggctt ttgccgcttt
     4381 aaacggtgaa aaggcattga ttccttatat tacggttgga gacccggata ttggggcgac
     4441 tttggcaata atgcacagta tggttgcaaa cggtgcggat attttagagt tgggcgtgcc
     4501 gttttccgat ccgatggcgg acgggccggt gattcagcgt gcggcggagc gggcgttggc
     4561 aaacgggatt tcgctgcgcg atgtcttgga tgtcgtcaga aaattccgtg aaaccgacac
     4621 gcaaacgccg gttgttttga tgggatattt gaaccctgta cataagatgg gttatcggga
     4681 gtttgctcag gaagccgcaa aggcgggtgt ggacggcatg ttgacggtgg attcccctgt
     4741 cgaaaccatc gatccgctct atcgcgagct gaaggataac ggggtcgact gtattttcct
     4801 gattgcgccg acgacgacgg aagaccgtat taaaaccatt gccgagctgg caggcggatt
     4861 tgtctattat gtttcgctca agggcgtaac gggcgcggca agtttggata cggatgaggt
     4921 ttcgcgtaaa atagagtatt tgcatcagta tatcgatatt cccatcggtg tcggtttcgg
     4981 catcagcaat gcggaaagcg cgcgcaaaat cggccgggtt gccgacgcgg tcattgtcgg
     5041 cagccggatt gtgaaagaga ttgaaaacaa tgcgggcaac gaggctgcgg ccgtcggtgc
     5101 tttggtcaaa gagttgaagg atgccgtgcg ctgattctgt cacgcattct aaatatttta
     5161 ggagttgtcc atgagctggt tagataaaat cctgccaccc aaaatcaaaa accgcagcaa
     5221 agacggttct tccaatgttc ccgagggttt gtggcacaaa tgtccgtctt gttcggcaac
     5281 cgtttattcg accgagttgc agcagaacaa tcaggtttgt ccgaaatgca accaccacaa
     5341 tccgttgtcg gcacgacaac gcctgaatct gcttttggat gaggatggca gagaggaggt
     5401 tgccggaaat gtcaaaccga cagatccttt gaagtttaaa gacggcaaaa aatatccgga
     5461 tcgtttgagt gcggcacgca agctgaccgg ggaagatgat gctttggtgg taatgaaagg
     5521 caagatgaac ggtctgcccg ttgtggttgc tgcgtttgag ttccgcttta tcggcggttc
     5581 gatgggttcg gttgtgggcg aacgattcgt acaaggtatc cgtcgggcgg ttgccgacaa
     5641 ttgtccgttc gtctgtgtgg cggcttccgg cggtgcgcgt atgcaggagg gtgtgaactc
     5701 gttgatgcag atgacgaaaa ccagtgccgc gctgcatttg ctgacggaaa aacgcctgcc
     5761 gtttatatcg gtattgaccg atccgactat gggcggcgta tccgccagct tcgcattttt
     5821 gggcgatgtc gtgcttgccg aacccaatgc attaatcggc tttgccggtc cgcgcgtgat
     5881 tgagcagacg gtgcgcgaaa cgctgccgga aggcttccag cgtgccgagt ttttgctgga
     5941 aaaaggcgca atcgaccaga ttgtcgaccg gcgcgagatg aagcagagaa tcagcggttt
     6001 gattacgctg ttgtgccgtc aggacaaagt ttctgccgcc tgatggctga tgaatcgaat
     6061 accgtctgaa accgatgttt cagacggtat ttttgtgtct ggttatttgt cgtgcggctt
     6121 catcgacggg gcatagcgtc cggcacgttc tttcaggcgt tgtaccaaac ctttcgtgtc
     6181 ggcgggtaca ccgccctcgc agaatgcctg atacaggacg gtgcgcagtg cgtcgttgcg
     6241 gcttaatgta ccgcctatcg gtttccattc ggcgtttcgg ggctgtatcc agcggcggtt
     6301 gaccgtgtcg ccgtagccga acactttata ggaggaaggt ttgccgttgc cgaacctgat
     6361 ggagaagcgg gcgcagaata tgccttcggc agtcaggttg tcgtagcctt tgtcggagcg
     6421 gatattgaga atgtagcgga tgctgccgtc gggcgcgggc ataatttgca ggctgtcgag
     6481 caggattttc ggctgtttgc cgtaattttc atccacataa atgtcaaacc agccgtccga
     6541 gtgcgtatcg ggcagcggcg gcagtttggc ggtatgttct ttaaattcgc gggcggcggc
     6601 ttcttcgggc gtttcgcggt agcgggtgtt gatcggcgtg tctttttggt tgaagccggc
     6661 agcaagggac gtgccggcgg tgagtgcgag cagcaggagg gcggtgcggc gcataagttt
     6721 ctccaaattg aaaacggcgt tattttatgg gttggcaaag ggggctgcaa gcaactgggg
     6781 tataatctcc ccccgattcc cattttttaa acggtacaaa cgatgaacag cgaaacttta
     6841 gacgtaaccg gattgaaatg tcccctgccg attttgcggg cgaaaaaggc tttggcgcaa
     6901 atgcagcagg gcgacgtgtt gaccgttctg gcaaccgacg gcggcgcacc gggggatttt
     6961 gaggcttttt gccgccaaac cggtcatgtg ctgttggatg cttccgaaca ggacggcgtg
     7021 ttcacgctgg tcgtcaagca caaataaatg ccgtctgaaa tgcggatgtc ccgcccgacg
     7081 gcgttgtttt tgaatatcgt atgtgccgcg tgccgtttca aaaacaaacc gtccggcgtg
     7141 cagtgtcggc atttcgggcg tatcgccgcc gatttgttcc ggaatggctg ttaaccgcct
     7201 tgccgtcggc aaagaagcaa aacccaagca caatcaaaat ctaaaggctg tgtttgaaga
     7261 ttccgttgat gcaacccaat cagtcggaca aaatgccttt catccaatgg aaccggtttc
     7321 cgaccggacg gaataaccgg ctttcccccg gcaaacggat ggaatcgacc gggtattcaa
     7381 acgcagccaa aacctaaaaa ggaacaacca tgcaaaccct gaccattatc cgccccgacg
     7441 atatgcacct gcacctgcgc gacggcgacg cgctcaaagc cgttgcccct tataccgccc
     7501 gccaaatggg gcgcgccgtc attatgccca acctcaaacc gcccgtcgtc agtgtagccg
     7561 acgcgcttgc ctacaaagcg cgcattatgg cggtgttgcc cgaaggcagc gcgtttgagc
     7621 cgctgatgac gctttacctg accgaccaag ccacgcccga gctggtgcgc gaagccaaag
     7681 ccgccggcat cgtcgccttc aaactctacc ccgccggcgc gacgaccaat tccgattccg
     7741 gcgtaaccga cctattcaag ctcatccccg tgttggaaga aatggcaaaa caggacatcc
     7801 tgttcctcgt tcacggcgaa gtaaccgacc ccgaaatcga cattttcgac cgcgaagccg
     7861 cctttatcga gcgcgtgatg aaacccgttt tggcgcaagt gccgaatctt aaagtcgtgt
     7921 ttgaacacat taccaccgcc gaagccgccc gcctggtttt ggaagcaggc gacaacgttg
     7981 ccgcctctgt gaccccgcaa cacctcctgc tcaaccgcaa cgacctcttg gtcggcggcg
     8041 tgcgccccca tcatttctgc ctgcccgtcc tcaagcgcga aacccaccgt caggcattgg
     8101 ttgccgccgt taccggcgag aaggcgcaca aattcttcct cggcacagat tccgcgccgc
     8161 acgccaaatc cgctaaagaa aacgcctgcg gttgcgccgg tatgttcagc gcgatgaccg
     8221 ccatcgagct ttacgccgaa gtgtttgaaa aagcaggcgc gttggacaaa ctcgaagcct
     8281 ttgcctcgaa aaacggcgca aggttttacg gcattccgga aaacgccggc acgatcaccc
     8341 tcgtcaaaca aagccaaacc gtccccgcaa gtgttcccta cggcgacggc gaacttgtcc
     8401 cgatgcgcgc gggcggcgaa atcggctgga cggtacagta ttgattgaac ggtcaagcaa
     8461 aatgccgtct gaaaggtttt tcagacggca tttgtgttgt tgactgattg atacgtcaac
     8521 ggcgtttgag tttgttacgt ttcggttatt tccgataaat tcccacaatt ttcaaatttc
     8581 gccattccca cgaaggcagg aatccggaaa ttcgatgcga ccagagttta tcaaaaacgg
     8641 cagcaactca aaaaaccgga ttcccgcctg cgcgggaatg acgagattga agtttcagaa
     8701 tttatttgaa atacccaaaa ttcaaaaaac caaattccca catgcgtggg aatgacgaaa
     8761 caaagaaagc agaaataagg acatagaact ttctttaaat ttgtgatgca tcaacggcgt
     8821 ttgggctcgt cggggcggat ttgggcagcg agtttgtcga ggatgccgtt gacgaatttg
     8881 tgcccgtccg taccgccgaa ggttttggta acttcgatgg cttcgttgat aatgacgggg
     8941 tagggcgttt cgggtatggc ggacagctcg tggcaggcgg tcagcaggac ggcgcgttcg
     9001 atggggttga ggtctttttc gtccctgtcg agcagcgggc ggatttgccg gatgtattct
     9061 gccgcattgg tttgcgtgcc gaagaaaagt ttgttgaaca attcttcgtc tgccttggca
     9121 aagtcggaca tttcgcggat gtttttagca atctcgggtg ctgcggtgcg gttgataagg
     9181 gattggtaaa cggcttgtac ggcaagctcg cgggaacggc ggcgggctgt tttcatgatt
     9241 tttccttgaa acggttgggc ggcacggtat gccgtctgaa acggaaaggg cgtattggtg
     9301 tacgccctgt ttgttattct tcgtcttcaa actgttcttc gagcagcagg ttgacgaggt
     9361 tggcgcattc tacggcgact ttggcggcat ccgaggcttt ttcttcaatc cgttcgattg
     9421 cctgcgcgtc gttttcggtg gttaagacgg cattggcaat cgggatgttg tagtcgagtg
     9481 cgacgcggct aacccctgct ccggattcgt tggaaaccag ctcgaaatgg taggtttcac
     9541 cacggatgac gacaccgatg gcaatcagtg cgtcaaattt ttcggaagag gcaagattca
     9601 tcagcgcgat ggggatttca agcgcgccgg gtacggtggc gacggtaatg ttttcgtctg
     9661 ccacgcccaa ttcttggagg atgcggcagc agactttgag catttcgctg ccgatttcgt
     9721 tggtgaagcg tgcctgtacg atgccgatgc ggaggtgttt gccgtcgagg ttgggggcga
     9781 tggtgttcat tgggtgtcct ttggtattcg gaggtttcgg aatgccgtct gaaggtttca
     9841 gtcttgcggc tgccagtcgg cgacggtttg gaatgtgccg tcttcggcaa gctcccatgc
     9901 gctgccttcg ggttgggaaa gcagtgcggc ggtttcaggg ttggttttgg cgatgtcggc
     9961 gaggctgacg atgctgaagt tgtccggatc gtcggtgtat tcgtcggtct cgtcgccgct
    10021 gaagaaacgc cagccgctgt cgttttcaaa aacgggggct tcgcggtaga ggaagccgac
    10081 gggccggttt tgtttggcga cggtgttggt ggcgatacag cggtcgagtg ccgaggaaag
    10141 tgcttgtgca aatgcgttca ttacgggaat acgttggggg aaaacttacg gattttacca
    10201 cgattcgtgc gttgtcggca gacggcggcg gtttggtggt acaatgtgcg ccgtttgcag
    10261 ccttaaggtg tttctgtatt tttggagtat ggaaacgcat tcgggctgtt ttttgcggaa
    10321 gacggtaatg aaagacgatg ttttgaaaca gcaggcacac gcggcgatac agaagaaact
    10381 gggctacgcg ttccgcgata tttcgctttt gcggcaggct ttgacgcaca ggagccatca
    10441 tgcgaagcac aacgagcggt ttgagtttgt cggcgattcg attttgaatt atacggtggc
    10501 gcggatgctg tttgacgcgt ttccgaagtt gaccgagggc gagttgtcgc ggttgcgggc
    10561 aagtctggtc aatgagggcg tgctggcgga aatggcggcg gaaatgaatg tcggcgacgg
    10621 tctgtacttg ggggcgggcg agttgaagag cggcggcttc agacggcctt cgatactggc
    10681 ggacgcgatg gaggcgatgt ttgcggcggt cagcttcgat gccgatttca acacggcgga
    10741 aaaggtggtg cgccatttgt ttgccgaccg cgtccggtgt gccgatttcc aaaatcaggc
    10801 aaaagacggc aaaactgctt tgcaggaggc gttgcaggca cgccgctttg ctttgccgaa
    10861 ataccgtatc gaagagcaaa tcggccatgc caacgacagt atgtttgtgg tttcctgcga
    10921 tttgggcgaa ctgggtttcg tgtgccgtgc caaagggacg agccgcaagg cggcggaaca
    10981 ggaggcggcg aaagaggctt tgaaatggct ggaagagaag ctgccgctga agaagaaaaa
    11041 gaaatgaggc ggcgcgtgaa tatgccgtct gaaatgtgga tatgaaagcg aatatggata
    11101 ttgaaacctt ccttgcaggg gaacgcgccg ccggcggata ccgttgcggc ttcgtggcga
    11161 ttgtcggtcg tccgaacgtg ggcaaatcaa cgctgatgaa ccatctcatc ggtcagaaaa
    11221 tcagcatcac cagcaaaaag gcgcagacga cgcgcaaccg cgtaacgggg atttataccg
    11281 acgataccgc gcagttcgtg tttgtcgata cgcccggctt tcaaaccgac caccgcaacg
    11341 cgctcaacga caggctgaat caaaacgtta ccgaggcgct tggcggcgtg gatgtggtgg
    11401 ttttcgtcgt ggaagcgatg cgctttaccg atgccgaccg tgtcgtgttg aaacaactgc
    11461 ccaagcacac gccggtcatt ttagtggtca acaaaatcga caaggacaag gcgaaagacc
    11521 gttacgcgct ggaggcgttt gttgcccaag tgcgcgccga atttgaattt gcggcggcgg
    11581 aggcggtcag tgcgaaacac ggattgcgga ttgccaacct gttggagctg ctcaagccgt
    11641 atctgcccga aagcgtgccg atgtatcccg aagatatggt tacggacaaa tcggcgcgtt
    11701 ttttagcgat ggaaatcgtg cgtgaaaaat tgttccgcta tttgggcgag gaattgcctt
    11761 atgcgatgaa cgtcgaagtg gagcagtttg aagaggaaga cggtttgaac cgcatctata
    11821 tcgccgtttt ggtcgataag gaaagccaaa aggcgatttt aatcggcaaa ggcggggagc
    11881 gtttgaagaa aatttccacc gaagcgcggt tggatatgga aaaactgttt gataccaaag
    11941 tatttttgaa ggtctgggtc aaagtcaaat ccggttgggc ggacgacatc cgcttcctgc
    12001 gcgagctggg tttgtagttt ttcttgctga actttacgca aatgccgtcc gaacaggttt
    12061 cagacggcat tttgtttcaa tcgggaatat ctttgttaaa aacgggttga tattatctgt
    12121 gca
//
LOCUS       DAWFAN010000049        12094 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWFAN010000049 DAWFAN010000000
VERSION     DAWFAN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12094
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.049"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(61..2361)
                     /gene="recQ"
                     /locus_tag="WGO65_000957"
     CDS             complement(61..2361)
                     /gene="recQ"
                     /locus_tag="WGO65_000957"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ0327265.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMCEGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADGAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFISSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKQMHGQSGIVYCLSRKKVEDVAQFLCENGLEAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGSEQTDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKIDRFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLTVLGQNAV"
     gene            complement(2426..3208)
                     /gene="trpC"
                     /locus_tag="WGO65_000958"
     CDS             complement(2426..3208)
                     /gene="trpC"
                     /locus_tag="WGO65_000958"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ0327266.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVPLEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRTRGVHTFLIGETFMRADDIEAEVGKLF"
     gene            complement(3259..4215)
                     /locus_tag="WGO65_000959"
     CDS             complement(3259..4215)
                     /locus_tag="WGO65_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ0327267.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGGSGSHYAVELMLAVRDRGWNGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQASA
                     AISAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLHGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRRTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHAGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            complement(4348..5886)
                     /gene="murJ"
                     /locus_tag="WGO65_000960"
     CDS             complement(4348..5886)
                     /gene="murJ"
                     /locus_tag="WGO65_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ0327268.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQVSLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFFLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            5944..6051
                     /locus_tag="WGO65_000961"
                     /pseudo
     CDS             5944..6051
                     /locus_tag="WGO65_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6345..7043
                     /locus_tag="WGO65_000962"
     CDS             6345..7043
                     /locus_tag="WGO65_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0327269.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQNPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(7100..8104)
                     /locus_tag="WGO65_000963"
     CDS             complement(7100..8104)
                     /locus_tag="WGO65_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ0327270.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            8174..10582
                     /locus_tag="WGO65_000964"
     CDS             8174..10582
                     /locus_tag="WGO65_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ0327271.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            10684..11712
                     /locus_tag="WGO65_000965"
     CDS             10684..11712
                     /locus_tag="WGO65_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0327272.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTASAKVAKVAKVAKVAK
                     VAATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKEAQISES
                     ELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQRRDFADNII
                     AEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAA
                     VGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFEEAVHAL
                     KPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVNLLRDLH
                     SGAYVDIR"
BASE COUNT         2807 a         3469 c         3386 g         2432 t
ORIGIN      
        1 cactataaaa acaggaattt ttaaatagag gcaatgccgt ctgaaacttg gtaacgggct
       61 tcagacggca ttttgtccca acaccgtcaa caccgccgca ccgtaacgtg cggctttttc
      121 ttcgcctacg ccatatacgg cggcaagctc cgccaaacct tccggctgtt tggcggcaat
      181 ggcgcgcagg gcggctttgc tgagaatgcg gtagggttcg gactgttcgt gttttgccgt
      241 ttcgccgcac cattggatca gggcgcgcat caggcggcgt ttgcgtttgg cggcttcatc
      301 gatgccgtct gaaaacggac ggcagacggc gaggatgtcc cgtccgtatt tggcggcgcg
      361 tacgctgccc aagccgtgta cgccttcgag gtcggtttcg gtttcaggcg tggcggcaag
      421 catatcggca aggctttcgt cggagaggac ggcatgcagg gcgcagtttt ccgcccttgc
      481 ctgttcatac cgccaggctt cgagtttttg acgcagttgc tgttcgcgtt cggtttgcgg
      541 acggatgacg gcatcgtggc taaagtcggc agcgttttgg cagactttga gtatgccgtg
      601 tccgaaacgg tcgattttgg cttcgcccaa gccgtagatg tcgtgcagcc cgttgaggtt
      661 ttgcggcatt ttttcgacaa ggtcgcgcag ggttttgtcg ccgaaaatca tataggcggg
      721 gatgccttcg gcttctgcct gtttcatacg ccaaacgcgc aatgcctgcc acaggcgttc
      781 ttcgcgttcg gtacgcagcc agttgtcttt gagggtgcgg gcggcgggct tgtcgcgctt
      841 gagcggacgc agcatcactt cggtttcgcc tttgaggact tttttggcgg cttcggtcag
      901 ttgcaatgcc tgatatcggg taatgttgac ggtgagatag ccgaggctga tgcactggcg
      961 gatgacgctg cgccattcct tgtcggtctg ttcgctgccg atgccgaagg tagacagttg
     1021 ttcgtgccgg ttgccgcgta tccaatcatc gcttttacca cgcaaaaggt tggtgatgta
     1081 accggcggca aaacgttgtc cggcgcggta cacgcagctg agtaattttt gtaccaacac
     1141 cgtgccgtca aaccgcacag gcggatgcag gcagttgtcg caatggccgc agggttcgga
     1201 tgcttcgccg aaatgtttga gcagcagtac gcggcggcag gcggcggttt cgcagacgga
     1261 aagcatggca tcgagttttt gcatttcgat ttgcttttgc acctcgtcgc tgttgccctc
     1321 ggcaatccgt tcgcgcagca acacccaatc gttcaaaccg taacacagcc aactcacggc
     1381 tggcagcccg tcccggccgg cgcgccccga ttcctgatag aaatgttcga cactttgggg
     1441 catatcgaga tgggcgacaa agcgcacgtc gggtttgtct atgcccatgc cgaacgccac
     1501 ggtcgccacc acgataatat tgtcttcatg cgtaaagcgg cgttggtttt cctcgcgcac
     1561 gtccatgctc aaaccggcat gatacggaat cgcctccaag ccattttcac acaaaaactg
     1621 cgccacatcc tcgacttttt tgcggcttag gcaatacaca atgccgcttt gcccatgcat
     1681 ctgcttgcgg atgaaatcca gcaattgttt tttaccatta tttttttcga taacctgata
     1741 ataaatattc gggcggtcaa agctggaaat aaattcgggc gcattgtcca agtgcagata
     1801 atgcttgatg tcggcgcgcg tggcggcatc ggcggtagcg gtcagggcga tgcgcgggac
     1861 gttcggatag cgttcggcaa gcatgccgag ctgttggtat tcggggcgga aatcgtgtcc
     1921 ccattggctg acgcagtgcg cctcgtcaat ggcgaacagg ctgacggttt gttggtcgag
     1981 aaaacgcaaa aagcggtcgg taactaggcg ttccggtgcg acataaagca gtttcagacg
     2041 accttgggca agcttgtcgg caatctcgcg cgccccatct gccgatgtgc cgctgttgac
     2101 tgccgccgct tcgatgccgg cggcgtgcag gtttgccact tggtcgttca tcaacgcaat
     2161 cagcggcgat acgacaaccg ccacgccttc gcacatcagc gcgggaatct ggtaacacaa
     2221 agacttgccg ccgcccgtcg gcatcagcac cgtcaaactc ccgccgcctg ccaaagtatt
     2281 gatgacagcc tcctgcctgc cgcgaaattc gggataacca aatacttcgt gcagaatctg
     2341 tttggcggtc ggtcggtgca tgatggttcc gtgctcggta agggtgttgg tcggttggcg
     2401 gcaatatgcc gtctgaaatc gggatttaga agagtttgcc cacttctgct tcaatatcgt
     2461 cggcacgcat gaacgtttcg ccgatcagga aggtgtgcac accgcgcgtc cgcataaatt
     2521 ccacatccgc cttacctgta atgccgcttt cggtaacgac ggttttgcct tccagcgcgg
     2581 gcagcagcga cagggtttgg tcgagggaga cttcaaaagt cctcaggttg cggttgttta
     2641 caccccacaa cggcgtggtc aggttgcggc atttttccaa ttcgctttca tcgtgcagct
     2701 cgagcaggac ggtcatgccc aattcgtgcg ccaccgcttc aaagcgttcc aattgttccc
     2761 gttccagtgc ggcggcaatc agcaggacgg catccgcccc ccaggcgcgc gcctgataaa
     2821 cctgatattc gtcgatgatg aagtctttgc gcagcacggg cagcgatacg gcttcgcgcg
     2881 cctgtttgag gtattcgggc gaaccttgga aatagggttc gtcggtcagt acggacaaac
     2941 acgccgcccc ggcgttttca taggcgcggg caatctcggc aggtcggaag tccggacgga
     3001 tcaggccttt gctcgggctt gcctttttga tttcggcaat gacggcgggc aggtttaggc
     3061 ggtgtttgcc gcgtatcgaa tcgatgaagc tgcggacggg cgcggcttct gcggcaagtg
     3121 cgcggatgtg ttcgagggga acggcggctt tttgggcggc aacttcctgt gccttggcgg
     3181 taaggatttt attgaggatg tcggtcatat tgtgttccgt attcgtccgg ggaaaggggg
     3241 aatattagca tcaaaccgtt aacgcctgtt tgtgcggaag ctgtcgaaat aggacaggac
     3301 ggtctgcggc agccattgca ggtgcagcct gccgccggtg ctgccgacaa agccggcgtg
     3361 tccgccgtgt gccggctgga acagggtaac ggcttcggac acttcgtccg gtgtcggcag
     3421 cgcttcgggc ggcaggaagg ggtcgttggc ggcattgagc aggagcagcg gtttggcaac
     3481 gtgtttgagc agcggtttgc aggaagttcg gcggtagtag tcgtgccggt cggcaaagcc
     3541 gtgcagcggt gcggtgaagc ggtcgtcaaa ctcgcccagt gttttgcacc ctgcggcaaa
     3601 tgccgtctga aaaccgtgga gcgaccgtgc tttgggtatc agggtgcgga ggaagtagcg
     3661 cgtgtagagc agccgtgtga tgccgctgtc gaagcggctg cctgccgcct ctgcatcgac
     3721 gggggcggag atggcggccg aggcttgcgg cagcgcgttt tcgccctgtt cgcccaaata
     3781 ttttgccagc gcgttgccgc ccagcgatac gccgacggcg tatatttcac ggtaacgcgc
     3841 ggcgagcgtg tccaaagtaa aggcgatttc ggcggtatcg cccaagtggt agaacactgg
     3901 ggcggtgttc gctacgccgc cgcagctgcg gaaatggacg actacgccgt tccaaccccg
     3961 atcgcgtacc gcaagcatca gttcaaccgc gtaatggctg ccactgccgc cctccaaccc
     4021 gtgaaacagc acgaccagcg gcgcatcggg cgaaatgccg tctgaaaagt cgtaggcgac
     4081 tttggtttta cccgtgctgt cgggaagcag ctcgcggcgg tatgtgggtg cggggcgttg
     4141 caggaatttg gcggcaatcg tgtcggcatt gccgttgcgg aggaaaaagg gcgtgtccgg
     4201 cggtgttaaa atcataaggt atcggttttc ttgttttcag acggcattga tgatgcggca
     4261 gcccgtccgg ctggtgcgga cgcgggggat gcgcgcccga atataggcgc ggaaaaagcg
     4321 ttttccgaaa aaggatatcg gcatcggtca gctttccacc cgtttgaaat ggcgcggacg
     4381 gaagcccaaa gccgccagag atgcgaaata cagtccgccg ccgacggcaa tcaggatgaa
     4441 gagccgggcg gccttttgca ttccaccggc gtgtgcccag tcgaacggca gccagatttg
     4501 ggcggcatac aggccgcctc ccatcacggc gagcgagagc agcatttttg ctaagaacgc
     4561 tgcccaaccc ttgccaggtt ggtaaatacc gtgtctgcgt aacaggaaga acaacaatcc
     4621 ggcattgatg cacgcgccca gaccgatggc aagcgaaagt ccgacgtgtt tcagtgggcc
     4681 gataaaggca aggttcatca actgcgtgca gatgagcgtg aagatggcga ttttgacggg
     4741 cgttttgatg ttttgccgcg catagaagcc gggtgccaac actttaatca taatcaaacc
     4801 gattaagccg aaagaatagg caatcagcgc gtgctgcgtc atctgcgcgt caaacagcgt
     4861 aaattcgcgg tacataaaca gtgtcgccac cagcgggaac gacaacaccg ccagtccgac
     4921 cgccgccggc agcgtcagca gcatacacag gcgcaaaccc cagtcgagca gggcggaaaa
     4981 ctgttccgta tcttggtttg ccgagtgttt ggacaaagtc ggcagcaaaa tcgtaccgag
     5041 tgccgccccc agcacgccgc tgggcagctc catcatgcgg tcggcgtaat acatccatga
     5101 aacgctgccc gattgcagat aagacgcgaa aatcgtgttg atcaccaaag aaacctgcgc
     5161 cacgctcacg cccaaaatcg caggcgccat ctgtttcatc acgcggttga ccgccgcatc
     5221 tttgaaattc agtttgggca atttcaaaaa acccagtttc gccaaccaag ggagctgaaa
     5281 gccgagctgc aaaataccgc cgacaaaaac cgcccaagcc agcgcggtaa cgggaggatc
     5341 gaaatacggc acgaaaaaca gcgcgaatac gataaacgac acgttcagga acgtgggcgt
     5401 aaacgccgga atgctgaatt tatgatagga attgagtacc gagccgacaa aagaggaaag
     5461 tgaaatcaat aagatatagg ggaacgtaat ccgcagcaaa tcgatggaga gctgaaattt
     5521 gtcggcatct ttggcaaaac cgggtgcgga aacataaatc acccaaggcg cggcaagtat
     5581 gcccagcgcg gtaacgatga ccagcacaaa cgacagcatc cccgccacat ggcggataaa
     5641 agcctccgtc gcctctttag aacgcgtttc cttatattcc gccaaaatcg gcacaaacgc
     5701 ttgggcaaac gccccctccg caaacacgcg gcgaagcagg ttgggcagtt tgaacgcgac
     5761 aaaaaacgca tccgtcgcca tgcccgcgcc gaatgcgcgc gcaatgaccg tatcgcgcac
     5821 aaatcccaaa acgcgcgaca ccatcgtcag gctgccgact tttgccaaag ctcccagcat
     5881 attcatcgta tttccttttc ggttgaaacc ccgcccttta gggcggcagg atcagacttt
     5941 gatagtggat taaatttaaa tcaggacaag gcgacgaagc cacagacagt acaaatagta
     6001 cggcaaggcg aggcaacgct gtactggttt aaatttaatc cactatattt gaaaggggtg
     6061 taacccttcc gaaccagggc aacgcatagg gcggtgcttt atgtgccgtc ctgtgcgttg
     6121 gaacatcatt agttccccaa cagccgtatc cgtctggggc gacggcgcgt attgtacgac
     6181 agaaaccgct tcagacggca tcgggtttga tgccgtccga agcggtttcc ccaaacgaaa
     6241 acgtcctttt ccggcggcaa actgtatcaa cacgcggaaa tgcaataaaa tagccggatt
     6301 ccgattgatt tccaacatct gtttccaaca tcacggagag ccgtatgaaa tccagacacc
     6361 tcgccctcgg cgttgccgcc ctgttcgccc ttgccgcgtg cgacagcaaa gtccaaacca
     6421 gcgtccccgc cgacagcgcg cctgccgctt cggcagccgc cgccccggca gggctggtcg
     6481 aagggcaaaa ctataccgtc cttgccaacc cgattcccca acagcaggca ggcaaagtcg
     6541 aagtccttga gtttttcggc tatttttgtc cgcactgcgc ccacctcgaa cctgttttaa
     6601 gcaaacacgc caagtctttt aaagacgata tgtacctgcg taccgaacac gtcgtctggc
     6661 agaaagaaat gctgacgctg gcacgcctcg ccgccgccgt cgatatggct gccgccgaca
     6721 gcaaagatgt ggcgaacagc catattttcg atgcgatggt caaccaaaaa atcaagctgc
     6781 aaaatccgga agtcctcaaa aaatggctgg gcgaacaaac cgcctttgac ggcaaaaaag
     6841 tccttgccgc ctacgagtcc cccgaaagcc aggcgcgcgc cgacaaaatg caggagctga
     6901 ccgaaacctt ccaaatcgac ggtacgccca cggttatcgt cggcggtaaa tataaagttg
     6961 aatttgccga ctgggagtcc ggtatgaaca ccatcgacct tttggcggac aaagtacgcg
     7021 aagaacaaaa agccgcgcag taagcccgtt tgaaaaatgc cgtctgaaac ttggttttca
     7081 gacggcattt tgattgggtt taaaacgtaa agcccgtttc cagttcttca tcgccgacca
     7141 gttcgaccaa gagcgcgtag agcggggcga gttcggcata acggcgcgat acgcggcgca
     7201 gatagtttaa gaaacgcggg atttcgggac ggtatttgtc tttgccgtca cggtagtaca
     7261 ggcgtgcgaa gatgcctgca accttcaagt gccgctgcac gcccatccat tcgaaccagc
     7321 ggtaaaactc gtcaaacgct tcggggacgg gcaagccggc agcccgcgcc ttttcccagt
     7381 agcggataac caagtccaag acaaattctt cttcccattc gataaaggca tcgcgcaaca
     7441 gcgacaccaa atcgtaggaa atcgggccgt aaagcgcgtc ttggaagtct aaaacgcccg
     7501 gtctgccgcg cgtcagcatc aggttgcgga cgataaagtc gcggtgcaca tagactttgg
     7561 gctgcgccaa caggggcggc agcagcgtat cgacggtttg ctgccaaagt tggcgttgtt
     7621 tgaatgttaa ttcgcgcccc aattcttttg cgacaaacca ttccgggaac aggttgattt
     7681 cgcgcagcat cgtttcacgg tcatattcgg gcaaaacccc ttcacggctt gccttctgca
     7741 attcgaccag ctcgccgatt gcctccaaaa gcagggcttt gtgcgccgct tcgccctgct
     7801 cctgaagcat cgcggtcaaa aacgtcgtat tgcccaagtc gttcaatacc acaaacccca
     7861 aatccgtgtc cgcgtgcaat acctgcggta cattgaccat gtcaaacagt ttctgcactt
     7921 tcaaataagg tgcgacactc atcttgtcgg gcggtgcatc catgcagacg acactgctgc
     7981 cgtctgaaaa cgttgcacgg aaatagcggc ggaaatcagc atccgccgcc gcgaaggaca
     8041 gatcgaagtc ccgttcggga tagatggtct gaagccaatt tttcagttcg gtttgccgtt
     8101 gcataacagt actaaagcat ttcaggttac aataaacgct attctaactg gcaaaccgac
     8161 ttgaggggcg attttggctc gtttattttc actcaaacca ctggtgctgg cattgggctt
     8221 ctgtttcggc acgcattgcg ccgccgccga tgccgttgcg gcggaggaaa cggacaatcc
     8281 gaccgccgga ggaagcgttc ggagcgtgtc cgaacccata cagcctacca gcctgagcct
     8341 cggttcgacc tgcctgtttt gcagtaacga aagcggcagc cccgagagaa ccgaagccgc
     8401 cgtccgaggc agcggcgaag catccatccc cgaagactat acgcgcattg ttgccgacaa
     8461 ggtggaagga cagtcgcagg tgcaggtgcg tgccgaaggc aacgtcgtcg tcgaacgcaa
     8521 ccggacgacc ctcaataccg attgggcgga ttacgaccag tcgggcgaca ccgttaccgc
     8581 aggcgaccgg ttcgccctcc aacaggacgg tacgctgatt cggggcgaaa ccctgaccta
     8641 caatctcgag cagcagaccg gggaagcgca caacgtccgt atggaaaccg aacacggcgg
     8701 acggcggctg caaagcgtca gccgcaccgc cgaaatgttg ggcgaagggc attacaaact
     8761 gacggaaacc caattcaaca cctgttccgc cggcgatgcc ggctggtatg tcaaggccgc
     8821 ttccgtcgaa gccgatcggg aaaaaggcat aggcgttgcc aaacacgccg ccttcgtgtt
     8881 cggcggcgtt cccattttct acaccccttg ggcggacttc ccgcttgacg gcaaccgcaa
     8941 aagcggcctg cttgttccct cactgtccgc cggttcggac ggcgtttccc tttccgttcc
     9001 ctattatttc aaccttgccc ccaatctcga tgccacgttc gcgcccagcg tgatcggcga
     9061 acgcggcgcg gtctttgacg ggcaggtacg ctacctgcgg ccggattatg ccggccagtc
     9121 cgacctgacc tggctgccgc acgacaagaa aagcggcagg aataaccgct atcaggcgaa
     9181 atggcagcat cggcacgaca tttccgacac gcttcaggcg ggtgtcgatt tcaaccaagt
     9241 ctccgacagc ggctactacc gcgactttta cggcaacaaa gaaatcgccg gcaacgtcaa
     9301 cctcaaccgc cgtgtatggc tggattatgg cggcagggcg gcgggcggca gcctgaatgc
     9361 cggcctttcg gttctgaaat accagacgct ggcaaaccaa agcggctaca aagacaaacc
     9421 gtatgccctg atgccgcgcc tttccgccga ttggcgcaaa aacaccggca gggcgcaaat
     9481 cggcgtgtcc gcacaattta cccgcttcag ccacgacagc cgccaagacg gcagccgcct
     9541 cgtcgtctat cccgacatca aatgggattt cagcaacagc tggggttacg tccgtcccaa
     9601 actcggactg cacgccacct attacagcct caaccgcttc ggcagccaag aagcccgacg
     9661 cgtcagccgc actctgccca ttgtcaacat cgacagcggc gcaacttttg agcggaatac
     9721 gcggatgttc ggcggcggag tcctgcaaac cctcgagccg cgcctgttct acaactatat
     9781 tcctgccaaa tcccaaaacg acctgcccaa tttcgattcg tcggaaagca gcttcggcta
     9841 cgggcagctc tttcgcgaaa acctctatta cggcaacgac aggattaaca ccgcaaacag
     9901 cctttccgcc gccgtgcaaa gccgtatttt ggacggcgcg acgggggaag agcgtttccg
     9961 cgccggcatc ggtcagaaat tctatttcaa ggatgatgcg gtgatgcttg acggcagcgt
    10021 cggcaaaaaa ccgcgcaacc gttccgactg ggtggcattt gcctccggca gcatcggcag
    10081 ccgcttcatc ctcgacagca gcatccacta caaccaaaac gacaaacgcg ccgagaacta
    10141 cgccgtcggt gcaagctacc gtcccgcaca gggcaaagtg ctgaacgccc gctacaaata
    10201 cgggcgcaac gaaaaaatct acctgaagtc cgacggttcc tatttttacg acaaactcag
    10261 ccagctcgac ctgtccgcac aatggccgct gacgcgcaac ctgtcggccg tcgtccgtta
    10321 caactacggt tttgaagcca aaaaaccgat agaggtgctg gcgggtgcgg aatacaaaag
    10381 cagttgcggc tgctggggcg cgggcgtgta cgcccaacgc tacgttaccg gcgaaaacac
    10441 ctacaaaaac gctgtctttt tctcacttca gttgaaagac ctcagcagtg tcggcagaaa
    10501 ccccgcagac aggatggatg tcgccgttcc cggctacatc cccgcccact ctctttccgc
    10561 cggacgcaac aaacgaccct gaccgtcgga aacccggcag gagcaccgtt cccgcacaag
    10621 acggcattcc accgacaacc ccaaacccgc catcaaaggt aggattcaaa cgataaggaa
    10681 agaatgatga aaatcaaagc cctgatgatt gccgccgcat tgctggcagc agccgatgtc
    10741 cacgccgcac cgcaaaaggc aaaaaccgca tccgccaaag ttgccaaagt tgccaaagtt
    10801 gccaaagttg ccaaagttgc cgccacggcg caaaaagaag ccgcacccgc acaacagcag
    10861 ggcggtatcc gcttttcaga cggcattgcc gccgttgccg acaacgaagt catcacgcgc
    10921 cgccggcttg ccgaagccgt tgccgaagcc aaagccaacc tgcccaaaga ggcgcagata
    10981 agcgaatccg agctgtcccg acaggtgctg atgcagcttg tcaaccaatc cctgattgta
    11041 caggcgggca aacgccgcaa cattcaagca agcgaagcgg aaatcgatgc cgtcgtcgca
    11101 aaaaattccg ccctcaaaaa cctcagcccc gcccaacgcc gcgattttgc cgacaacatc
    11161 attgccgaaa aagtccgcca gcaggcagtg atgcagaaca gccgcgtgag cgaagctgaa
    11221 atcgatgcct tcctcgagca ggcgcaaaaa caaggcatca ccctgcccga aggcgcaccg
    11281 ttgcgccaat accgcgccca acacatcctg attaaagccg acagcgaaaa cgccgccgtc
    11341 ggcgcggaaa gcaccatccg caaaatctac ggagaggccc gcagcggcac agacttttcc
    11401 agcctagcgc gccaatattc gcaagacgcg agcgcgggca acggcggaga tttgggctgg
    11461 tttgccgacg gcgtgatggt tcccgccttt gaagaagccg tccacgcgct caaacccgga
    11521 caggtcggcg cgcccgtccg cacccaattc ggctggcata tcatcaaatt gaacgaagtg
    11581 cgcgatgccg gcacacctca ggaacgtatc cgcaattccg tgcggcaata catcttccaa
    11641 caaaaagccg aacaggcaac cgtcaacctg ttgcgtgacc tgcattccgg cgcgtatgtc
    11701 gacatccgct aaggcggttt gaagcaaaaa gccataccga tcggcaaaaa tccgggcggt
    11761 atggcttttt ggatttcgag ttacttttac accgtcattc atcattcccg cgaaagcggg
    11821 aatctagaaa cgaaaagtaa caggaattta tcgggaatgg ctggagttta aaggactgga
    11881 ttcccgccgt cgcgggaatg acgggatttt gggttgtggt aatttatcgg aaaaacaaaa
    11941 aaacctatgc cgtcattccc gagcaggcgg gaatccggtt atttaaaact gcagaaattt
    12001 atccgaagca acaacaatct ttccatcgtc attcccgcgt aggcgggaat ctagcaacga
    12061 aaagtaacag gaatttatcg ggaaaacaga aacc
//
LOCUS       DAWFAN010000050        12075 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWFAN010000050 DAWFAN010000000
VERSION     DAWFAN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.050"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            188..622
                     /locus_tag="WGO65_000966"
     CDS             188..622
                     /locus_tag="WGO65_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0327273.1"
                     /translation="MNFALSVITFTLASFLPVPPAEAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(671..2014)
                     /locus_tag="WGO65_000967"
     CDS             complement(671..2014)
                     /locus_tag="WGO65_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ0327274.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(2019..2672)
                     /locus_tag="WGO65_000968"
     CDS             complement(2019..2672)
                     /locus_tag="WGO65_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ0327275.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2823..3113
                     /gene="gatC"
                     /locus_tag="WGO65_000969"
     CDS             2823..3113
                     /gene="gatC"
                     /locus_tag="WGO65_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ0327276.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3176..4621
                     /gene="gatA"
                     /locus_tag="WGO65_000970"
     CDS             3176..4621
                     /gene="gatA"
                     /locus_tag="WGO65_000970"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ0327277.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4618..5550
                     /locus_tag="WGO65_000971"
     CDS             4618..5550
                     /locus_tag="WGO65_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ0327278.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5593..7023
                     /gene="gatB"
                     /locus_tag="WGO65_000972"
     CDS             5593..7023
                     /gene="gatB"
                     /locus_tag="WGO65_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ0327279.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGEIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7115..8125
                     /locus_tag="WGO65_000973"
     CDS             7115..8125
                     /locus_tag="WGO65_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ0327280.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDISINIPGYKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSSRVVHFYWGARHQDDLYALEEAQGLADRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8255..8887)
                     /gene="pdxH"
                     /locus_tag="WGO65_000974"
     CDS             complement(8255..8887)
                     /gene="pdxH"
                     /locus_tag="WGO65_000974"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ0327281.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9109..10158
                     /locus_tag="WGO65_000975"
     CDS             9109..10158
                     /locus_tag="WGO65_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0327282.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKGETRKTATQAY
                     GQKASDGIKPQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIYEQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(10227..11768)
                     /locus_tag="WGO65_000976"
     CDS             complement(10227..11768)
                     /locus_tag="WGO65_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ0327283.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKSGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMIFVKA
                     LDLFS"
BASE COUNT         3082 a         3669 c         3001 g         2323 t
ORIGIN      
        1 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatattttt gggaatctgt
       61 tttaccccaa tatataaagc accatattaa ggcggagttt cttccccact ttgaaccgaa
      121 cccggaaaag acaccgccca agccaatcct gatgctgccc cggcagccaa ccatcaagga
      181 aaccctaatg aactttgctt tatccgtcat cacatttacc ctcgcctctt tcctgcccgt
      241 cccgcccgcc gaagccgccg tctttacttg gaaggacggc ggcggcaaca gctattcgga
      301 tgtgccgaaa cagcttcatc ccgaccagag ccaaatcctc aacctgcgga cgcgccaaac
      361 caaaccggcg gtcaaacccg cccaagccga cgcagggaag cgcacagacg gcgcggcaca
      421 ggaaaacaat cccgacactg ccgagaaaaa ccggcagctt gaggaagaaa agaaaagaat
      481 tgccgaaacc gaacggcaga acaaagaaga aaactgccgg atttcaaaaa tgaacctgaa
      541 agcggtggga aattcaaatg caaaaaacaa ggatgatttg attcggaaat acaataacgc
      601 cgtaaacaaa tactgccgtt aatcggctct agcgcaaacc ccatgccgtc tgaaacggca
      661 cggggtttgt ttatttctgc cagtaggttt tgacgttgac gaactcgtac agcccgaatt
      721 cggacaattc gcgcccgtaa ccggaatctt tgactccgcc gaaaggcagg cgcaaatcgc
      781 tgctggtatg gcggttgata aacaccgatc ccgcctgtat tttttcggca aaccgccaag
      841 cgcgttcggt atcggcggta taaatgcagg cacctagccc gaacggggaa tcattggcaa
      901 gggcgatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc ccgaatactt
      961 cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg ggataaaacc
     1021 agcctcgccc ttgtgggatt tttccgccgg tcaggcacac cgcgccgttt gaaacggcat
     1081 cttcaacctg cccgtgaacc cggtcccgca aatcttcgcg gtgcagcggt gcaagcgtgg
     1141 tatcgggatg tttcgggtcg cccattttca atttggcgca ttcggcgaga aacagctgaa
     1201 taaagcggtc ggctgcggct tcggttacga tgatgcgctt ggcggcgtta cacgattgcc
     1261 ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc gcgttccaaa tcggcatcgg
     1321 gcatcacgat aaaggcgttg ctgccgccga gctccaacac ggttttcttg aggtttgcgc
     1381 ccgcatgtgc cgcaagaatg cgccccgtat gcgtcgaacc ggtaaacgcc atcgcatcgg
     1441 tatcttcgac tgccttgagc gtgtccgcct catccagcca cgcgcctgcc agaggaatac
     1501 cgtctgaagc caaatcgaac aacgcccgac tgacgcgtgc cacgctgggc gcgggcttga
     1561 cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc tgccagacgg
     1621 gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcgaagcgc acctgactca
     1681 aactcgcctg cgtcgcgatg gttttgtggg caagcagttc gggggcaagg cgggcgtaat
     1741 agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcaag cagcgtccga
     1801 cttcctcaca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg tcggcaaatt
     1861 tttgcaggcg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg ccgcgcattt
     1921 tcaaatccgc cagccgccgt tcaaactccg cataatcttg agcggggcgg cggtaaagcg
     1981 tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag ttgcgggaat
     2041 atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat catacccata
     2101 atgcggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt cccggcaacg
     2161 attaaaatgg cataacaaat cgcactgacc accaaaccgg ccgcgataat caacgcgatg
     2221 tcgccgtatg ttttagccgc cgaagcgtaa ataatcacgg tcgaaatacc gcccgggccg
     2281 atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg ggcggggcgc
     2341 gcctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc gttgccgttc
     2401 atcatcgaaa tggcgatcag cagcaccaaa atcccgccgc cgacctgaaa cgaaccgacg
     2461 ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc aatcacggca
     2521 aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct gtgcccgttg
     2581 gtcaggtcaa ggtaaagcga caacgcgcta aacggattaa tcagcaccaa aaaagccaca
     2641 atcagcttgc cgatttccat acccaatccc attatttccc cctccttcaa acccgtgcgg
     2701 caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa ttaaaaattc
     2761 aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca aggatgcttt
     2821 tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga ctgcacctga
     2881 ctgcggaaga aaaagaaaaa tcgcttcaag aattaaacga cattttcact atggtcgaac
     2941 agatgcaaac cattaacaca gacggcatcg aaccgatggc gcacccgcac gaggccgccc
     3001 tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat caggcgggtg
     3061 ctccggaagt acgcaaccgt ctgtacatcg taccgcaagt tatcgaagaa taatccgaat
     3121 atgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa gatttatgac
     3181 ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt ccgccgtcga
     3241 actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca acggctatat
     3301 caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac gtatcgcgca
     3361 gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt tctgccaaac
     3421 cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc cctacaccgc
     3481 caccgtcgtc caaaacctgc tcgacgaagg tatggtaacg ctcggccgca ccaatatgga
     3541 tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggtgcagcca aaaacccatg
     3601 gaatcttgag cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg ttgccgcgcg
     3661 cctcgcccct gccgcgctcg gttcggacac cggcggctct atccgccaac ccgcatcgca
     3721 ctgcggcatt accggcatca aacccacata cggcacggtt tcccgcttcg gtatggtcgc
     3781 ctacgcctcc agcttcgatc aaaccggccc gatggcgcaa actgccgaag actgcgcgat
     3841 tctgttaaac gcgatggcag gtttcgaccc caaagactcc accagcctcg agcgcgaaaa
     3901 agaagactac acccgcgatt tgaaccaacc gctcaaaggt ttgaaaatcg gcctgcccaa
     3961 agaatatttc ggcgaaggca acagcgccga tgttctgacg gcattgcaaa acaccattga
     4021 tttgctgaaa gcccaaggcg cggaattgat tgaagtttcc ctgccgcaaa ccaagctgtc
     4081 catccccgcc tactacgtcc tcgcctccgc agaagccagc accaaccttt cacgttacga
     4141 cggcgtacgt tacggacacc gtgccgccca attcgccgat ttggaagaaa tgtacggcaa
     4201 aacccgcgcc gaaggtttcg gcagcgaagt caaacgccgc atcatgatcg gcacttatgt
     4261 actgtcgcac ggctactacg atgcctacta tctcaaagcc caaaaactgc gccgcctcgt
     4321 tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc cgaccgcacc
     4381 cactgcagcc ccaaaaatcg gagcggatgc ttcgccggtt gaaacctact tgagcgatat
     4441 ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg caggcttcag
     4501 cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg aagccaaaat
     4561 cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac gacccgaatg
     4621 aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta tgcgccttta
     4681 tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa agaaatttac
     4741 gccttcgagc agtttgtcaa cgcctcccct atccgtcagg ggctgttcct ccactgcccg
     4801 caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg caaacgccgt
     4861 ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac agacatcctg
     4921 caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga cggcatcagc
     4981 ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt atctttgcgc
     5041 gacgaagcag gcaaccggct gtatatggcg actttcgcct ttgtcggcac acacctgctg
     5101 acagcctccg tacaagggcc tgcgggtgaa gaagccaaag acactgtccg ccgcataacc
     5161 aaacaacttc acggcttgcg tccccaacaa ctgatggtaa ccgccctgca atatttcgcc
     5221 gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt caaactgcgc
     5281 tggaaactta aaaagcgtgt caaaatgaat tacgacgcat tctggcagga atacggcgca
     5341 agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa agaccttgcc
     5401 gacatcgaaa gcaaaaaacg ctcgatgtac cgcaagcgtt atgaaatgct ggacaacatg
     5461 gttgcagaga tgaaggacgg tctgaaaaca gaagcacgcg gcatttcaga cggcatccaa
     5521 acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga tattttagag
     5581 aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt ccaattgaac
     5641 accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc caacgcgcac
     5701 gccggcgtgg tggaatgtgc tttgccgggc gtactgccgg taatgaaccg tgaagtcgtt
     5761 gaaaaagcca tcaaattagg cttggctttg gatgcgaaaa tcaaccggaa aaacgtgttc
     5821 gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag ccagttggac
     5881 ttaccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt gaaaaccatc
     5941 aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtacatga aggcttgaac
     6001 ggcgcgaccg gtatcgacct gaaccgcgcc ggcacgcctt tattggaagt ggtgtccgaa
     6061 cccgaaatgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca cagcttggta
     6121 acctggctga acatttgcga cggcaatatg gcggaaggtt cgttccgcgt cgatgccaac
     6181 gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat taaaaacctc
     6241 aattccttcc gtttcttgga gcaggcgatt aattacgaag cagaagcgca aatcgagatt
     6301 ttggaagacg gcggcaaagt acagcaggca acgatgctgt ttgatcccga aaaaggcgaa
     6361 acccgcgtaa tgcgcctgaa agaagacgcg caagactacc gctacttccc cgaccccgat
     6421 ttgctgcccg tcatcatttc agacgctcaa atgcaaaaag ccaaagcaga aatgcccgag
     6481 ctgccgaaag aaatggcagc gcgtttcgtg gcggactacg gcgtgtccga atacgacgcg
     6541 cgcctgctca ccgcaagccg cgcacaggcc gcctattttg aagaagccgc caaagagagc
     6601 ggacaaggca agccgactgc caactggatg aacggcgaac tcgccgccgc actgaacaaa
     6661 gaaggcatgg aacttgccga cagcccgatt accgctccgc gcctcgccgc gctggtaggc
     6721 aaaatcgccg acggcacatt aagcagcaaa ttagcgaaaa aagcctttga agccatgtgg
     6781 gcagaacccg aagccaccat tgccgaaatc atcgaaaaac acggtttgca acagatgacc
     6841 gacaccggcg agattgaagc catggtggac gaagtgctgg caaacaacgc caaagccgtg
     6901 gaacagttta aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca agtgatgaag
     6961 gccagcaaag gcaaagccaa ccccgcgcag gttcaagagc tgattaaagc caaactggcc
     7021 tgataagcaa gatgccgtct gaaacccaga tccgacgttc agacggcatt ttcacagtct
     7081 gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccgtta ccctgcccga
     7141 ccaaaccacc tttgccgccg gcgacggtga aaccgttttg gccgctgccg cccgtcaaaa
     7201 cctcaatctg ccccattcct gcaaaagcgg tgtctgcgga caatgcaaag ccgaactggt
     7261 cagcggcgat attcaaatgg gcggacactc cgaacaggct ttatccgaag cagaaaaagc
     7321 gcaaggcaag attttgatgt gccgcaccac cgcgcaaagc gatatcagca tcaacatccc
     7381 cggctacaaa gccgatgccc tacccgtccg caccctgccc gcacgcatcg aaagtatggt
     7441 tttcaaacac gatgtcgccc tcctgaaact tgccctgccc aaagccccgc cgtttgcctt
     7501 ctacgccggg caatacattg atttactgct gccgggcaac gtcagccgca gctactccat
     7561 cgccaattcg cccgaccaag aaggcatttt ggaactgcac atccgcagac gtgaaaacgg
     7621 tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag gcattgtccg
     7681 cgttaaaggc ccgctcggtt cgtttacctt gcaggaagac agcggcaagc ccgtcatcct
     7741 gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc ttatccgcca
     7801 aggcagcagc cgcgtcgtcc atttctactg gggcgcgcgt catcaagatg atttgtatgc
     7861 cctcgaagaa gcacaagggt tggcagaccg tctgaaaaac gcctgcttca cccccgtatt
     7921 gtcccgcccc agagagtgct ggcaaggaag aaaaggccac gtacaagaca tcgcggcaca
     7981 agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggctctccgg ctatgaccga
     8041 acaggcaaaa aatctgtttg tgcaacagca taagctgccg gaaaacttgt ttttctccga
     8101 cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg ctttccgttc
     8161 ggaacacaaa aaacttcccg tccgtatttt ccccgtgaaa aaatgccgtc tgaaacccga
     8221 ttcctgtttt cagacggcat atgttttttc ctgttcaagg tgacagccgc tcgcgtatcc
     8281 agccgccatc cagcaaacgg tattggatgc ggtcgtgcag cctgctcggc ctgccctgcc
     8341 aaaactcaat caaatcggga atcacaatat agccgcccca atgcggcgga cgcggcacgt
     8401 gcaaaggatg tttgagtccg accgccgccg cctttgccac caataccgcc ttgttcggaa
     8461 tcacttcgct ctgcgcgctt gcccacgcac ccaaacggct ctgatacggg cggctctcaa
     8521 aatattcgtc cgacaatttc tccgccagcc tttcaacacg cccttccacg cgcacctgac
     8581 gctccagctc cggccaaaaa aacgtcatcg ccgcaaatgg atgagcatcc agcgaacgcc
     8641 ccttgcggct gtgataattc gtaaagaaaa caaaaccttc agaattaact tccttcagca
     8701 gcaccatacg gctgttgggt ctgccgcgtc cgtcgaccgc cgccacatta accgccgtcg
     8761 gctcgttaac ctgtgcgcgt accgcctcgt ccaaccaccg ctcgaactgc tcgatcggat
     8821 tgtcggcgca atcggcttcc gacaattccc gtttgctgta atcttcccga atattgtgca
     8881 aatccatgtt accgctcctc ttgttatttg aaagattcta cccccgcacg caaaccgatt
     8941 tcaaccgttg cacaaacttt gtccccaaag ccgcagcgac gatttcatcc gcaaaaccgc
     9001 cgcatcaggt acaatatcga accgtccgac tgaggacggc attttatcaa cccgtcctgc
     9061 cgcatacgcc gcagaagaac cgctttatca agcgagttag gaaaaatgat gtccaaacag
     9121 cccaccagca aacgccaatg gcgcgacggc gcagccccgt ctgccaagaa aaccgccaaa
     9181 cctttcaaaa gcaaagcccg tcccaaaggc gaaacgcgca aaaccgccac ccaagcttac
     9241 ggacaaaaag cttcagacgg catcaagccg caaaacgccc ccaaacagcg cgccgccaaa
     9301 gccaaaaaac tcgtcgtccg caatcccaac caaaaaatta tggaacacgc gcgcgatttg
     9361 aaagaacgcc gcagcgacct gtcgcgcatg gaacccgaac gcctgcaaaa agtgcttgcc
     9421 gcgtccggcg tcggctcgcg ccgcgaaatg gaagaatgga ttaccaacgg ctggataacg
     9481 gtcaacggca aaaccgcgca actgggcgac aaagttaccc ccgacgacca cgttaccgtc
     9541 aaaggcagca tcatcaagct caaatgggcg gaccgcctgc cgcgcatcat cctgtattac
     9601 aaacaagaag gcgaaatcgt ttcccgtgac gacccgcaag gccgcgtcag catattcgac
     9661 cgcctgccgc aggccgccag cagccgctgg gtcgccatcg gacgcttgga catcaacacc
     9721 agcggacttc tgattcttac cacctccggc gaactcgtcc aacgtttcgc ccaccccagc
     9781 ttcgaagtcg aacgcgaata cgccgtgcgc gtcttgggcg ggctgaccgg cgaacaaatg
     9841 cgcgtcctca ccgaagaagg cgtgatgctc gaagacggct tggcaaaagt cgaacgcatc
     9901 tacgaacaag gcggcgaagg cgcgaacaaa tggtacaacg tcgtaattaa agaaggccgc
     9961 aaccgcgaag tgcgccgcat attcgaaagc caaggcctca ccgtcagccg cctcgtgcgc
    10021 gtcggcttcg gtcccatcgg actgcccaac cgcctcaaac gcgggcagtt ctacgaactc
    10081 aatcccgccg aagtcgccaa catcatcaaa tgggcggaca tgctgctgcc gggcgaacgc
    10141 cgccgcaaaa aagcctaaac ccgccaaaac acaaaaatgc cgtctgaaac atctactgtt
    10201 tcagacggca ttttgttcgg gcgttttcag gagaaaaggt cgagtgcttt gacaaagatc
    10261 atcaccacgc cgtaggcgag cggtatgacg gccaatgccc aacgccacca gaccgatatg
    10321 ccgccgccgg aaactttttc gcccacaagg taagcgtcgg atatggcggt ttcgtcatcg
    10381 gggttgccgc tgtgtgcggc ggttttgatg tctttttcgt ggtgtttttc gtgtacggat
    10441 ttgacggcga ggttgcacaa caaaccgata atcagcagac ccgccatgat gtacatggtt
    10501 acgctgtatg cctgtgccgc cggtatgccg ctgtcgattt ggctttggcg tatgtaattg
    10561 accagtaccg ggccgatgac ggcggcggtt gaccaggcca gcaggatgcg cccgtgaatc
    10621 gcgccgacct gataggtgcc gaacaggtct ttcaggtagg cgggaatggc ggcaaatccg
    10681 ccgccgtaca tggaaataat cacgcaaaag ccgatgatga acagggcttt gctaccaccc
    10741 tcgccgatgg agggaacggc gaaatacagc agcgaaccga gtacgaagaa aatggtgtag
    10801 gtgtttttgc gtccgatttt gtcggaaacg ctcgaccata aaaagcgtcc gcccatgtta
    10861 aacaggctaa ggaggctgac gaagcctgcc gccgcacctg caccgactgc cgcctgcctg
    10921 cctacggagg tttcggaaaa gagttcctga atcatcacgg atgcctgacc caatacgccg
    10981 atgccggcag ttacgttcag gcacaatacc cagaacaaca gccaaaactg cggcgttttc
    11041 atggcttggg acacgttgac atgattgctg ctgaccagct tgttttgcgt tttcggcgcg
    11101 gtatagcctt caggtttcca gccgtcggca ggtacgcgga tggtaaacgc gccaaacatc
    11161 atcagtgcga ggtaaagcag acccaatacg gcgaaggttt cggcaacccc aaccgaagca
    11221 gcgtttgaaa aggcgttcat cagtgatacg gaaagcggcg aggccagcat tgcgccgccg
    11281 ccgaaaccca taatcgccaa accggtcgcc atacccgact tgtcgggaaa ccatttcatc
    11341 agtgtggaaa ccggcccgat gtagcccaaa cccaagccta cgccgccgat gacgccgttg
    11401 cccaaataga gcaggaagag gttgtgcgta cgcacgccga atgcggatac gaagaagccc
    11461 aggctgaagc agcaggcggc ggcaaatatg gctttgcgcg gccctacccg ttccatccac
    11521 gtaccgaaca gggcggccga cgcgcccagc atcgcgagtg cgatactgaa aatccaacct
    11581 acggtcgtca gcttccaatc tccggccgcc gattcggtta tgccgataag tttggtcagc
    11641 ggcgcgttga atacggaata ggcgtaaatc tgcccgatgg caaggtgcac cgccaatgct
    11701 gcgggcggta cgagccaacg gttgaaaccc ggcttggcaa tgcttgcctc acggtctaaa
    11761 aacttcataa catcctcttt ctgtcagttg aaaaataaaa tttcatttgc tcaatggaaa
    11821 cttattgaaa actataaaaa aatatcggat cggtttttta tccgccccaa gatgccccgt
    11881 ctgaaacatt tcgggtgtac ggaaaggttt ctgttttttc cgacaaattc ctgcggcttt
    11941 tcgcttccgg attcccgctt ttgcgggaat gacgaattaa agattatctt aaggtcaagg
    12001 gactggattc ccgctttcgc gggaatgacg gcggcgggtt gctgtttttt gtttataaat
    12061 ttctgtaact ttgga
//
LOCUS       DAWFAN010000051        11761 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWFAN010000051 DAWFAN010000000
VERSION     DAWFAN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11761
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.051"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            46..261
                     /locus_tag="WGO65_000977"
     CDS             46..261
                     /locus_tag="WGO65_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327284.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMSESPDFANAVAMICIVWIAVL"
     gene            complement(259..374)
                     /locus_tag="WGO65_000978"
                     /pseudo
     CDS             complement(259..374)
                     /locus_tag="WGO65_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            590..1891
                     /gene="bioA"
                     /locus_tag="WGO65_000979"
     CDS             590..1891
                     /gene="bioA"
                     /locus_tag="WGO65_000979"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ0327285.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLHDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMAHFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGLLTELADKTVHILKEHNK"
     gene            1888..2535
                     /gene="bioD"
                     /locus_tag="WGO65_000980"
     CDS             1888..2535
                     /gene="bioD"
                     /locus_tag="WGO65_000980"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ0327286.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKLVQTGC
                     QNIADDIAVHRKIMGIPMQEADKQGLTMPEIFSYPASPHLAARLDGRDLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2550..2732
                     /locus_tag="WGO65_000981"
     CDS             2550..2732
                     /locus_tag="WGO65_000981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327287.1"
                     /translation="MEHLYDEYGNTTPNRRTTNKKDETISSKVTVAAIPVAAPFAIAD
                     IVDQDFIQAIMKITGH"
     gene            2756..3142
                     /locus_tag="WGO65_000982"
     CDS             2756..3142
                     /locus_tag="WGO65_000982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327288.1"
                     /translation="MKCYIKVVIIVFIMVMTTSCNPCFFATSSCDYSFRKNYPINSDD
                     EYYVKNNTSKETRIADLRYCISYACPEVYQKYQLPDENGDYREIIRRKYKDDYKKGYK
                     NADDVMTCYGKIGECMKNNKGYSTKW"
     gene            3136..3579
                     /locus_tag="WGO65_000983"
     CDS             3136..3579
                     /locus_tag="WGO65_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327289.1"
                     /translation="MVMFRLLSTALVLLIMQGCYYTTIDNEKCFVAPQSVHCPVSSDI
                     ENVIRKNPNKSINQVRSDIKYCANMYFSNIRELNIPQDFDNYIFMLKEKGIPRNDKEL
                     AYNAVRNMHIICLSSLGGYDYYQKTAEFQKIDYKKEMEKLLNENH"
     gene            4037..4513
                     /locus_tag="WGO65_000984"
     CDS             4037..4513
                     /locus_tag="WGO65_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ0327290.1"
                     /translation="MGYGFEEWLPDLPAPVSDGISLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            4543..5433
                     /gene="ubiA"
                     /locus_tag="WGO65_000985"
     CDS             4543..5433
                     /gene="ubiA"
                     /locus_tag="WGO65_000985"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ0327291.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVLPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGATWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            5618..6067
                     /gene="ptsN"
                     /locus_tag="WGO65_000986"
     CDS             5618..6067
                     /gene="ptsN"
                     /locus_tag="WGO65_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ0327292.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            6069..7031
                     /locus_tag="WGO65_000987"
     CDS             6069..7031
                     /locus_tag="WGO65_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ0327293.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSICTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            7012..7866
                     /gene="rapZ"
                     /locus_tag="WGO65_000988"
     CDS             7012..7866
                     /gene="rapZ"
                     /locus_tag="WGO65_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ0327294.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIAYLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPKLRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            7947..8888
                     /gene="ylqF"
                     /locus_tag="WGO65_000989"
     CDS             7947..8888
                     /gene="ylqF"
                     /locus_tag="WGO65_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ0327295.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            8989..9591
                     /locus_tag="WGO65_000990"
     CDS             8989..9591
                     /locus_tag="WGO65_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ0327296.1"
                     /translation="MTDKISPDALIEAALLTQTEPLNEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVIQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPTTVPSDTLPN"
     gene            9775..11448
                     /gene="recN"
                     /locus_tag="WGO65_000991"
     CDS             9775..11448
                     /gene="recN"
                     /locus_tag="WGO65_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ0327297.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPTLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            11558..11634
                     /locus_tag="WGO65_000992"
     tRNA            11558..11634
                     /locus_tag="WGO65_000992"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11592..11594,aa:Arg,seq:acg)
     gene            11652..11726
                     /locus_tag="WGO65_000993"
     tRNA            11652..11726
                     /locus_tag="WGO65_000993"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11685..11687,aa:Glu,seq:ttc)
BASE COUNT         3053 a         2781 c         3045 g         2882 t
ORIGIN      
        1 ggctgtaacc ttcaatcgga atttgttgcc tgcgggatat gcggtatgaa tgtttgggat
       61 atatggggca ggatggtgga aatctatcat aagtataaga agccgtgcct ggttttggcg
      121 gtggattttg tgatgggtat ggtattcata gagccgaatg aggagccgtg catcggcaga
      181 tgctatgcgc caatgtcgga gtcccctgat tttgccaacg ctgttgctat gatctgtatc
      241 gtatggattg ccgttttata gtggattaaa tttaaaccag tacggcgtta cctcgccttg
      301 ccgtactatt tgtactgtct gcggcttcgt ctccttgtcc tgatttttgt taatccacta
      361 tatctatgac tgattgaagc gttgggcgga ggctgtgtga aacggtattg ggcgttgggt
      421 cgtctgattc caatcaggct tgaggaatgc gaaacggtgt gcgcttatgc tgaggacgat
      481 ttgtttcgcg gttttgcgcc cgaaacggat ggaaaggtgt gggaagcggt ctgtcggagt
      541 agaatacgcg tttgcgtttg aatacagtaa gaagaaaaga gagaaactta tgccgtctga
      601 acatcaacac acatcatcat tgcttgattt cgaccgtacc catctgcttc atccctatac
      661 ttccatgacc gatccgctgc ccgtttatcc tgtcaaacgt gcagaagggg tgtttatcga
      721 attggcggac ggcacgcggt tgattgacgg gatgtcgtct tggtggtgtg cgatacacgg
      781 ctacaatcat cctgttttga atcaagcggt tgagacgcag atgaaacaga tgtcgcatgt
      841 gatgttcggc ggtttgacgc acgaaccggc agtggagctg ggcaaattat tggttgggat
      901 tttgccgcag ggactggacc gtatttttta tgcggattcg ggttcggttt cggtagaagt
      961 cgcgctgaag atggcggtgc aataccagca ggcgcggggt ttgaccgcga agcagaatat
     1021 tgcgacggta cggcgcgggt atcacggcga tacttggaac gcgatgtccg tctgcgatcc
     1081 ggaaacgggg atgcaccata ttttcggcag cgcgttgccg cagcgttatt ttgtcgataa
     1141 tccgaaaagc cgtttcgacg atgaatggga cggggcggat ttgcagcctg tccgcgcctt
     1201 gtttgaagtg catcatgcgg atattgccgc ctttatttta gagccggtcg tgcagggcgc
     1261 gggcggcatg tatttttatc atccgcagta tcttcgcgga ttgcacgatt tgtgcgacga
     1321 gtttgatatc gttttgattt ttgatgaaat cgccaccgga ttcgggcgca cgggcaagat
     1381 gtttgcctgc gaacacgcgg aggtcgtgcc ggatattatg tgcatcggca agggtttgag
     1441 cggcggctat atgacgctgg cggcggcaat cacttcgcaa aaagttaccg aaacgatttc
     1501 gcgcggcgag gcgggcgtgt ttatgcacgg accgacattt atggcaaacc cactggcgtg
     1561 tgccgttgcc tgcgcttcgg tcaaactgct tttgtctcaa gactggcagg caaatatccg
     1621 ccgcattgaa agcatcttaa aaggccgtct gaaagccgcg tgggacattc gcggcgtgaa
     1681 agacgtacgc gttttgggtg ccatcggcgt gatcgagctg gataaaggcg tagatatggc
     1741 gcattttcaa gcggattgcg tggcgcaggg tatttgggtg cgccccttcg gcaggctggt
     1801 gtacctcatg ccgccttaca ttatttcaga cggcctcttg accgaacttg ccgataaaac
     1861 cgtacacatt ttgaaggaac acaacaaatg aaaggcgttt acttcgtcag cggcattgat
     1921 acggacatcg gcaaaaccgt cgccaccggc gtattggcaa aacaattgtt gcagcaaggc
     1981 aaaagtgtga ttacgcaaaa gctcgtgcaa accggttgcc aaaatatcgc cgacgacatc
     2041 gccgtccacc gcaaaatcat gggcataccc atgcaggaag ccgacaaaca agggttgacc
     2101 atgcccgaaa tcttcagcta tcccgcctcg ccccaccttg ccgcccgtct ggatggcagg
     2161 gatttggact tggacaaaat ccgtaccgcc acacaagaat tggcggcgca gtacgaagtc
     2221 gttttggtcg aaggcgcggg cggattgatg gttccgctga cggaaaacct gttaaccatt
     2281 gattatatcc gtcagcaagg ctatcccgtc atcctcgtta ccagcggacg gctcggcagc
     2341 atcaaccaca ccctgctcag tttcgccgcg ctcaaacaat acggcattcg cttgcacagc
     2401 ctcgtgttca accacatcca cgacagccgc gacgcacaca tcgcccaaga cagcctgagc
     2461 tacttgaaat gccgtctgaa agccgatttt tccgaagcgg aatggatgga gttggcaaaa
     2521 acagacgcgg tataaagatt gggaaaaata tggaacacct atatgatgaa tatggaaata
     2581 caacgccaaa tagaagaaca acaaataaaa aagatgaaac tatttcaagt aaagtaactg
     2641 ttgcagcgat accagtagcc gctccatttg ctattgctga tatagtcgat caggatttta
     2701 tccaagcgat tatgaaaatt actggtcatt gattatctat atattggtgg ttgttatgaa
     2761 atgctatatt aaagttgtca ttattgtttt tataatggta atgacaactt cttgtaatcc
     2821 ttgctttttt gcaacttctt cttgtgatta ttcttttaga aaaaactatc ctataaatag
     2881 cgacgatgaa tattatgtta agaataatac gagcaaagaa acaagaattg cggatttacg
     2941 ctattgtatt tcttatgcat gtccagaagt ttatcaaaaa tatcaactac cagatgaaaa
     3001 tggagattat agggagatta ttcgtagaaa atataaagat gattataaaa agggctataa
     3061 gaatgcagat gatgtcatga catgctacgg aaagattgga gaatgcatga aaaataataa
     3121 aggatattca acaaaatggt aatgttccgc ttattatcaa cagcccttgt attgttgata
     3181 atgcagggct gttattacac aactattgat aatgaaaaat gctttgttgc cccacaaagc
     3241 gttcattgtc ctgtaagttc tgatattgaa aatgttatta gaaaaaatcc taataaaagc
     3301 ataaatcaag ttaggagtga tattaaatat tgtgctaata tgtatttttc caatatacgg
     3361 gaattaaata ttccgcaaga ttttgataat tacattttca tgcttaaaga aaaaggtatt
     3421 ccaagaaatg ataaggaatt ggcttataat gcagttagaa atatgcacat tatttgtttg
     3481 agttctttgg gtggttatga ttattatcaa aaaaccgcag aatttcagaa aatagattat
     3541 aaaaaagaaa tggagaaatt gttaaatgaa aaccattaaa tttgctattg ccgatatagt
     3601 tgatagcgac tttatccaag tgattatgaa attaagcggg cattgattta tgattacgcg
     3661 ttgttttaaa gttgtcatta attgttttta taatggtaat gacaacttct tgtaatcctt
     3721 gattttttgc aacttcgtct tgtaattatc cttttgatga tatgacgata agagcaaaca
     3781 acgatattga atattttgtt aaaaataata cgactaacga acaacgaatt gatgatttca
     3841 gaagttgcct ttccgttgct tgtcctgaaa tatataaatt atatcaatta cctgatgaaa
     3901 agggtaatta tcaatatatt ataaaaaaat attatgataa taaatacaat gataatgcag
     3961 tcaaatcgac aaattgtatt ggggataatg caagatgtat gcttaataat aaaggatatt
     4021 caacaaaatg gtaataatgg gttacggctt tgaggaatgg ctgcccgacc tgcccgcccc
     4081 tgtttcagac ggcatcagcc tgccgatgag ccggttattg aaagcccggt cgctgaccgc
     4141 cgcattgcgc gccttgccgc atacattttc ggtggaactg ctgaaactgg gcgaattgga
     4201 gacggaatgc ggagggaggc tggtgcgcga agttttgttg aagctggacg gtatccctgt
     4261 tgtcgcggca aggagcgagt gccggatagg ttcggcgttt tggcaaaaca ttttggactg
     4321 cggcacgcgt cctttgggcg agcgtctgtt tcaagccgat ttggaagggg cgcgttcggc
     4381 gtttgagttt gccgtttccg gcgaaggatg cggacggtac tttgccgcgc ggcgttctcg
     4441 gttttcccgt cacggcgagg aaatgctgct gaccgagtat tttctgcccg aactgaaacg
     4501 ctttatcgga taaaataccg ttttttcaag ctgtgcggca atatgaatcc taaatcccct
     4561 ttatttttac gactgtccga tcgtttggat gtgtacctgc gcctgatgcg ggcggacaag
     4621 cccattggga cgctgctttt gctgtggccg acctactggg cattgtggct ggcttcagac
     4681 ggcattcccg atttggcggt attggcggcg tttacaatcg gcacgttttt aatgcggagt
     4741 gccggttgcg tcatcaacga ttttgccgac cgcgattttg acggtgctgt cgagcgcacc
     4801 aaaaaccgtc cgttcgcaca gggtcgggtc ggaaaaaaag aagcgctgct gctgacggca
     4861 tttttgtgcc tgcttgccgc attgtgcctg attccgctga atcatctgac atggctgatg
     4921 agcctgcccg cgctgtttct tgcgctgact tacccgttta ccaaacgctt ttttccgatt
     4981 ccccaactct atctcgggct tgccttttcc ttcggtatcc cgatggcgtt tgccgccgtt
     5041 gccggaaacg tgctgcctca agcgtggata ctctttgccg ccaatgtgtt atggactctg
     5101 gcgtatgaca cggtttatgc aatggcggac aaagaagacg atttgaaaat cggcatcaaa
     5161 acctccgccg tcacgttcgg gcgttacgac atcgccgccg ttatgctgtg tcacggaggc
     5221 tttaccctgc tgatggcagt attgggtgcg gttatcggtg cgacatgggc atattggacg
     5281 gcaatcccca tcgtcctgct gctgcaatac cgccaatatg ccgccatcaa aagccgcgtc
     5341 cggcaaatct gttttgaaac gtttttggca aacaacagaa ttggttgggt gtggtttacc
     5401 gccatttttg cccatacgtt tttcgcgaaa taaggcaggg caatgccgtc tgaagagccg
     5461 taaactgctt tggacggcat ttctatctgt gccgaaaagc gttaaaatat gtttttaaaa
     5521 cgctgtgtta tgtcagcccg tgccgtatgc gggattgaga tttgccccgg cagtcggtac
     5581 aatctttctg ttttgcgatg tctgaaaaga gaagcttatg agccttatcg gcgaaatttt
     5641 gcctttgtcc catattgttt tggatatgga ggtgggcagt aaaaaaaggc tgtttgagga
     5701 agcgggcctg cttttggaac gcgaatcctc attgtcccat gctgatgttt tcgaatgtct
     5761 ttttgcccgt gaaaaactcg gttcgaccgg tttggggcag ggcgttgcca tcccgcacgg
     5821 ccgtcatgcc ggcgtgaagc aggcgacggg cgcgttcatc cgcacgcgcg aacccgtcgg
     5881 atttgacgca ccggacggca aaccggtttc cctggttttt atcttgctgg ttccggaaaa
     5941 tgcgacggga gagcatttgg aagtattgtc caaactggcc ggcaagtttt cccaaaaaag
     6001 catcagagaa tcgctgatga ccgtttcttc tgcggaagaa gcccgcgcca tcctgactga
     6061 agaataatat gcccagtatc tctgtccgcc ggctgtttga cgacaaccaa tacaaactgc
     6121 aacttgcctg ggccgccggc aattcgggtg cggacaaccg tatcggcgta gaggcggaca
     6181 agcccgtcct cgccttggtc ggacacctga atttcatcca ccccaaccaa atccaagtgg
     6241 tcggtttggc ggagtcggaa tatctgaacc gtctcgaatc gggggaaacg ggttatcagt
     6301 tcggagacct gttcgacatt tctatgtctt tggtcattgt ggcaaacggc ttgccggttt
     6361 ccccgggtct gcgcgactat tgccataaaa acgatattcc actgctgact tccaaactcg
     6421 aaagccccta tctgatggat gtgttgcgga tttacctgca acgcaccctg gcggcatcgt
     6481 ccgtcaaaca cggcgtattt ctcgatgtgt ttgaaatcgg cgtgctgatt accgggcatt
     6541 ccggcctggg taagagcgaa ttggcattgg aactgatttc gcgcggccac agcctgattg
     6601 ccgacgatgc ggtcgagctg ttccgcatcg gcccggaaac gctggaaggg cgttgttcgc
     6661 ctatgctgcg cgattttttg gaagtgcgcg gcttggggat actcaatatc cgccatattt
     6721 tcggcgaaac ttccatccgc cccaaaaaaa tcctgcaact cattatcaat ttagtcgagg
     6781 cggacgacga gtatatgaag cagctcgacc gtttgagtat ctgtaccgaa accgaatcca
     6841 tcctcaacgt caacgtccgt tcggtaacgc tgcctgtcgc ggtcggacgc aaccttgccg
     6901 tattggttga agcggcggtg cgcaattata ttcttcagtt gcgcggtaag gacagtacgc
     6961 gcgaattttt ggaacgccat cagacacaac ttaaagaaaa cgaacagaat catgaaaatc
     7021 gtcctgatta gcgggttgtc cggttcgggc aaatccgtcg cactgcgcca aatggaagat
     7081 tcgggttatt tctgcgtgga caatttgcct ttggaaatgt tgcccgcgct ggtgtcgtat
     7141 catatcgagc gtgcggacga aactgaattg gcggtcagcg tcgatgtgcg ttccggcatc
     7201 gacatcggac aggcgcggga acagattgcc tatctgcgcg gactggggca cagggttgaa
     7261 gttttgtttg tcgaggcgga agaaagcgtg ttggtccgcc ggttttccga aaccaggcga
     7321 ggccaccctc tgagtaatca ggatatgacc ttgttggaaa gcttaaagaa agaacgggaa
     7381 tggctgttcc cgcttaaaga aatcgcctat tgtatcgaca cttccaagat gaatgcccaa
     7441 cagctccgcc atgcagtccg gcagtggctg aaggtcgaac gtaccgggct gctggtgatt
     7501 ttggagtcct tcgggttcaa atacggcgtg ccgaacaacg ccgattttat gttcgatatg
     7561 cgcagcctgc ccaacccgta ttacgatccc aagttgaggc cttacaccgg tatggacaag
     7621 cccgtttggg attatttgga cggacagccg cttgtgcagg aaatggttga cgacatcgaa
     7681 aggtttgtta cgcattggtt accgcgtttg gaggatgaaa gcaggagcta cgttaccgtc
     7741 gccatcggtt gcacgggagg acagcaccgt tcggtctata ttgtcgaaaa actcgcccga
     7801 aggttgaaag ggcgttatga attgctgata cggcacagac aggcgcaaaa cctgtcagac
     7861 cgctaattcc gtcaaaccat tatgccgtct gaaaccccgt cctccgtttc agacggcatt
     7921 tcaaatattg aaaagaaaga acagacatgg caatccaatg gtttcccggc catatgaaca
     7981 aggcgaaaaa agccatcgcc gagcgtgcaa aaagcgttga tatggtgatt gaaatgctgg
     8041 acgcgcgtat gcccgcctcc agcgaaaacc ccctgcttgc ccagctttcc aaaggcaaac
     8101 ccaaacttaa aatcctaaac aaacaagatc ttgccgaccc cgaacgcaca aaggtctggc
     8161 ttgaccacta caacagccgc cccgacacgc gcgccatcgc cctcgattcc tccgaaaccg
     8221 gcgcacacgg caaaatcacc caagcctgcc gcgcgatgat tccccaccgg caaggcatag
     8281 acaaacccct gcgcgttttg atttgcggca ttcccaacgt cggcaaatcc accctcatca
     8341 acggcatgat aggcaaaaaa tccgccaaaa ccggcaacga acccggcatc accaaagccg
     8401 aacaacgcct tttccttgcc gacgacttct ggctgtacga cacccccgga atgctgtggc
     8461 cgaaaatcat tgtcgaagaa agcggctaca acctcgccgc cggcggttca gtcggacgca
     8521 acgcattgga cgaagaagaa gtcgccctcg aacttttaga ctacctccgc cgccactacc
     8581 tccctatgtt gcaagaacgc taccaagccg acaaagaccc cagcagccac tgggacgaaa
     8641 acgtttggct cgaatggata gccaaaaaac gcggcgcagt cctcagcggc ggacggatca
     8701 actaccaaaa agccgccgaa aacatcctca ccgacttccg tgaaggcaaa atcggcagaa
     8761 tcaccctcga aacgccgaac caatgggaaa cttggctcaa aaaagcccgt cagaaagaag
     8821 ccgaactcaa agccatacgc gaagccagaa aagcagagcg taaagggcag aagccttcgg
     8881 aagcataaag aatgccgtct gaaaaatatt tttcaggcag cttctctcta ctacaaccga
     8941 tttcagacgg catatccaaa cccatgccgt ttcagcacgg atacccgtat gaccgacaaa
     9001 atttctcccg acgcgctgat tgaagccgca ctgctgaccc aaaccgaacc gctgaacgaa
     9061 aaatctatgc gcgaattgtg tgtgccgccg ttgtcgcaag acaaactgat tgatgtgttg
     9121 gcgcagttga aaacgcgttg gcaggatagg gcgttgcaac tggtgcatac gcaagagggc
     9181 tggcgttttc agattgttca gacggcattc gagcggctgg gcagcctgca agaacagcgt
     9241 gcgccgcgct actcccgcgc cgtgatggaa acactggcga ttatcgccta ccagcagccc
     9301 gtaacgcgcg gcgacatcga aggcatacgc ggtgtggcgg tgtctcagaa cgtgatccag
     9361 accttgcagg atcgggggtg gattgaagtc atcggacatc gggacacatt gggaaaaccc
     9421 gcattgtggg caacaacggc aacgtttctc agcgatttgc gcttggacgg tctagaagaa
     9481 cttcctccgc tgaccgaact gggcgaactg gttttgcccg atttaatgga aatgccgcct
     9541 acggatgaag aagagccgac aaccgtacca tccgataccc tgcccaactg aaattccaaa
     9601 tgccgtctga aacgcacatt gcttcagacg gcattgcaac aaataagcag ataaaaacaa
     9661 gcactaagaa aaattaagga aaaacttatt tttaatttaa aaaatcttag ttataattcg
     9721 tatatctaaa gttgatattg cttttgtcgg tagaattgct aaggaatcct cacgatgctt
     9781 ctaacacttt ctttgcgtga ttttgtcatt gttgaaaatc tgaatctgga ttttcaaagc
     9841 ggctttaccg tattgaccgg agaaactggc gcgggcaagt ccattacttt ggatgcgatt
     9901 ggtctgctgt tgggcgataa agccgattac agccaagtcc gcagcggcgc aaaagaagcg
     9961 cagttgtcgg cattgttcga tatttcccat ttgcctactt taaaagcaga attgtatgaa
    10021 caggggcttt taaacgacgg agaagaagaa ctcagtatcc gccgcattat cgatgccaaa
    10081 ggcaaaagcc gcagctttat caacaatcag gccgctacct tggcgcaact caaagccgtc
    10141 ggcagccagc ttatcgacat ccacgggcaa aacgcccatc attcgcttaa tcaggaagcc
    10201 gcccagcgcg aattgttgga cgcatttgcg ggcagcaggg cgcaggcgga aaccgtcagg
    10261 cagctttatc aaaactgggc aaactcaaaa aaagcattgc aggaagcgca ggaacaggca
    10321 gaaaacattg cactcgaaca agagcgtctg gaatggcagt ttaacgaatt gaatcagttg
    10381 gacattaaac aaggcgaatg ggaagccctc agccaaagcc acgacagcct tgcccattct
    10441 gccgagctgt tgcaggctgc cgaagaagtc ggaagcaaga ttgacggcga caacggcatc
    10501 caacgccata tctatcaatg tcaaaaacta ttggccaatc tgcaaaacat cgagccgcgc
    10561 tttgccgaga gcctgaatat gttggcaagc atcgaggccg aattgggcga aatcagtgcc
    10621 aatatgcgcg atgtggcagg acgcagcgac atcaacccca atgaattggc ggcacaggaa
    10681 cagcgtatgg gcgaactgat ggggatggcg cggaaatacc ggatcgagcc tgaagagttg
    10741 cctgccaagt tggcagaaat cgaagaacgc ctgcaaagcc tgcaagctgc cgccgatttg
    10801 gacgcgctcg agcataatgt tgcccacaat tttgccgaat atcaggaagc tgcccacatc
    10861 ctttctgcca tgcgccatca ggcggcagag cgtttgagcg gcgaaacgac cgagcatatg
    10921 caacaccttg ccatgaaagg tgcacgtttc gacatcgtcc tgttgccctc gtcgccgacg
    10981 gcacacggtt tggagcaggt tcaatttcaa gttgccgcca acaaaggcaa tccgccccgt
    11041 ccgctgaata aagttgcctc cggcggcgaa ttggcgcgta tcagccttgc cttacaggtt
    11101 gttgccagcc aatataccca agttcccacc ctgatttttg atgaggtcga taccggtatt
    11161 ggagggggag tggctgaaat ggtcggcaag gcattacgtg cgttgggcag aaaacatcag
    11221 gtgcttgccg ttacccatct tccgcaagtc gcatcctgcg gcgaaaacca ctggcgggtg
    11281 cgcaagcaca gcgagggaga gcaaaccgtc agcgaaatca gtatattgga tgaaatccaa
    11341 cgggtcgaag aggttgcccg tatgttgggc ggagaagtca ttaccgatac gacgcggcag
    11401 catgcggcag aattgctgca acttgcgtcg aaaaatagtt tattttaaaa tcaatcagtt
    11461 aaaaaataac taaaaataaa agtctaaaac aatagacaga actcagataa atccgtatta
    11521 tcacgctttc ttaatcactt gaacaagtga ttgtgctgca cccgtagctc agttggatag
    11581 agtatctggc tacgaaccag agggtcgggc gttcgaatcg ctccgggtgc gccagtaaga
    11641 aaatacaata tgcgcccatc gtctagcggt taggacatcg ccctttcacg gcggtaaccg
    11701 gggttcgatt ccccgtgggc gtgccaattc aaaatgcctc cgattatatc ggaggcattt
    11761 c
//
LOCUS       DAWFAN010000052        11697 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWFAN010000052 DAWFAN010000000
VERSION     DAWFAN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.052"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(309..731)
                     /locus_tag="WGO65_000994"
     CDS             complement(309..731)
                     /locus_tag="WGO65_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ0327298.1"
                     /translation="MQVTSKWIDGMCFVGTTESGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(849..1520)
                     /locus_tag="WGO65_000995"
     CDS             complement(849..1520)
                     /locus_tag="WGO65_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ0327299.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1536..2162)
                     /locus_tag="WGO65_000996"
     CDS             complement(1536..2162)
                     /locus_tag="WGO65_000996"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ0327300.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGVVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2269..3075)
                     /locus_tag="WGO65_000997"
     CDS             complement(2269..3075)
                     /locus_tag="WGO65_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0327301.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAASEPAAASAAQGDTSSIG
                     STMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMM
                     KFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQP
                     TKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYI
                     PSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3334..3774)
                     /locus_tag="WGO65_000998"
     CDS             complement(3334..3774)
                     /locus_tag="WGO65_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ0327302.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3837..5243)
                     /locus_tag="WGO65_000999"
     CDS             complement(3837..5243)
                     /locus_tag="WGO65_000999"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ0327303.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5413..6528
                     /gene="lptF"
                     /locus_tag="WGO65_001000"
     CDS             5413..6528
                     /gene="lptF"
                     /locus_tag="WGO65_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ0327304.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6525..7595
                     /gene="lptG"
                     /locus_tag="WGO65_001001"
     CDS             6525..7595
                     /gene="lptG"
                     /locus_tag="WGO65_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ0327305.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7905..10490
                     /gene="acnB"
                     /locus_tag="WGO65_001002"
     CDS             7905..10490
                     /gene="acnB"
                     /locus_tag="WGO65_001002"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ0327306.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10632..11627
                     /locus_tag="WGO65_001003"
     CDS             10632..11627
                     /locus_tag="WGO65_001003"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ0327307.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2685 a         3259 c         3041 g         2712 t
ORIGIN      
        1 caataaatta atatatggct taaaataacg ggatgcccgc cttccgcccg cccgcagaag
       61 caggcgggta tcattttaaa acgcggcatt taaaatttga ccgaaaattg ttgacaatcc
      121 ggaatcaagt ctgcacaata ccccgacaag tccaagtatt ataaaggctg aataaagagg
      181 aaacagcagg cagatatatt cgggaggtgc agtccgaata tatctgcttt tttatgcgcc
      241 tccggattgc ctgccgcacc tttcccttca gacggcatca gccgtttccc cataatgccg
      301 cccgatgcct atttatctgc cccggcaatt tcaaaactgt gggtaatctt tgccgctttg
      361 cccaacataa tcgaagccga acagtatttt tcggcagaca tctgaacggc gcgctcaatg
      421 gccgattctt tcaaatcatg cccgaatact ttgaaatgga tgtggatttc ggtaaacacg
      481 cgcggcgcat cgtccgcccg tttcgccgta accgtcgcac ggcagtcagt cactttctga
      541 cgctgttttt cggcaatcat caccacatcg atgctcgaac agcccgccac gcccaacagc
      601 agcatttcca aagggctggg cccgcgcttg gccgcacctt ctgccgccga accctccata
      661 acgacgctgt gcccgctttc cgtcgtgccg acaaagcaca ttccgtctat ccattttgat
      721 gtaacctgca tggtgtcatt cctgaaaata gcgttaaaac cgctttgcat atggcgttat
      781 tgtaaacaat ttcaagcggc ttatgcagaa atatggacaa aacggcgaaa aaacgcctga
      841 aaaccgattt acggtttggc ggactggcca ttgatctgca ccgatttgag tttcagcgta
      901 taggttttgc cgtcgtcggt atagccgatt tgtgccggaa tattgttcag ggacggtgcg
      961 aagaaataca ttaccgtatc gtcgccgcgc cgcacccgat atttgacgac ttcggtttcc
     1021 acgccgccta tgctgtattt tcctgtaccc gccttattca aaccgccgac ggaataaagt
     1081 tttttgccgt tggtgatttt cagccccggg gggagtttcg cgtcatttgc cgccaactgc
     1141 caggcaagcg tgaacaaatc catagccttg gggctttgct cggttttgct ctcgcccgct
     1201 ttgccgtagg ttacgctgcc gtcggcgaat ttggcttccg catacagttt gcccctgcgt
     1261 atgtctctat agtaggtagg gtgcagggta ttgccgacaa ccgtaccgcc ggactcgaaa
     1321 cggatattgt atagcggcac tttaatcgtc gaaacgattt tgtaagcatt gccgctgcgt
     1381 tcaaatgtca tcgtggcggg aatgccgtag ctgccggaat agtgcagcac ggcggattgg
     1441 ggcagccctg ccgcatacgc gcacggcagg gcggcggaca aaatggcggc ggaaaatata
     1501 tttttaaaag tcttcatcat ttgctcccgc ccggtttacg ccgtcagaaa acgggcggca
     1561 tcggcgtttt ccgaatttct gacgcggttt ccctcgataa tcaggcggcc ggcggcaaaa
     1621 tcggcaacgg ctttcggata gagtttatgc tcgacagcca aaacccgggc ggcaacatcg
     1681 tctgccgtat cgccgtcgag tatcggcaca accccttgcg atacaatcgg gccgcaatcc
     1741 agttcggcag taacgaaatg gatggtgcag ccggcaacgc gacaacctgc ctccaaagcg
     1801 cgttcgtgcg tatgaagtcc ggtaaacgag ggaaggatgg acgggtgaat gttcatcagc
     1861 ctgccttcgt aatgggcaca gaactcaggg gtcagaatcc gcataaaacc tgccaaaacc
     1921 accaagtcgg gttgatatgc gtcgattttt tccatcatgg cggtatcgaa ggcaagtcgt
     1981 gattcaaagt ttttatgatt caggctgtcg gtcggaatac cctgaccggc cgcccattgc
     2041 aaaccggcag ccgtttcgct gttgctcaac acggcggcaa tacggacgtt gggaatggcg
     2101 gcattgacga ttgcctgcat attgctgccg cgtccggaaa tcaggatgac gatgtttttc
     2161 ataatggtgc gcttttgaaa gggatgccat ctgaaccgct gtttggtggt ttcagacggc
     2221 atttgccgta aaaatgcccg aaaacctgtt tcgggcatgg attcggactt aatttacttt
     2281 tttgatgtcg acttgagccg gctgcttggc gggcgcgttt tcgggtgcgc cgattttgac
     2341 cagtttcaca tcaaatacca aagtggcgtt cgggccgatt ttgtcgcccg caccctgttc
     2401 gcggtaggca aggttggacg ggatgtagaa cgtggcttcg ccgccttctt tcagaagctg
     2461 tacgccttcg gtccaacccg gaatcacttg gctcaaaggg aaggtgaccg ggccgccgtt
     2521 ggctttgctg ctgtcgaata ccgtaccgtc aatcaggcgg ccttcgtatt ccacggtaac
     2581 gatgtcgtct ttggtcggct gtttgccttc gccctgtttg gtgattttgt attgcaggcc
     2641 ggaagcagtg gtcttcacgc cgtctttggc ggcattttct ttcagaaagg cttcgccttt
     2701 ttctttattg gccttcgcgt ccgccttgtg tttttctacg gctttagcct gttgttcctg
     2761 aaggaatttc atcatgactt cctgagcctg ctcttcagtc attttgattt ctttgccgtc
     2821 atacactgcc tgcatggctt cggtaaagac tttcaaatcg atttccgcac cctgttcctt
     2881 catttgcttc agggagcgtc cgatgtccac gcccatcgca tagcttgcct gctgcatcgt
     2941 gctgccgatc gaagaggtgt cgccctgcgc ggcagaagcg gcggcaggtt cggatgcggc
     3001 ttcttttttg ccacaggcgg aaagtgccaa agcggcggaa agggtcagtg cgctgatttt
     3061 gaaaatggtg ttcatgatgg atcttcgctg tcgataaggt cggaaaaacg ggattatagc
     3121 cgagtttgaa tgtttcaaca cacaggatga cacataaagc gtcaatcgtg tgttgccctg
     3181 ttttggaagg gattgaacct tccaaaataa gttttgattc taccgccccg agggacagat
     3241 gtccaagtgg cggggttcaa ccgataagga aattttaatc aaatagaatc aagcctgttt
     3301 aaattttgta aatgcggcat ttcagacggc attttatgcc ttgccctcca tgccgtgatg
     3361 ttcgatggca aaaccgcttc ggcggtaggc ggtaaagcgt tcgcgcgcgt cggcgagatc
     3421 ttccaagctg ttgccgacga tttccaaaac gcgcgcgggc aagaccggag cggtgttcca
     3481 aaaaccgtcg gacaggttca aaacagtcat gccttcggga attcggggcg gattgccgcc
     3541 gcaagcaagc aggacggatg tttcagacgg catggcttct tccatttccc aaatttcgtg
     3601 cggaatgaaa ctctcggctt cgtattgcca aagcattttg tccaattcct gaagccgccc
     3661 gaacgaatcg gaccacacca gtatcctgcc gccgtcccga atcgcacggg caatcaggcg
     3721 gcaggtgaaa atcggaactt gggcaacgtg cgtgtaaaag gtggctttcg gcatattgtt
     3781 tgagcatttg gcaggacgat gccgtctgaa aggcttcaga cggcattgtg gcaaaattaa
     3841 agattccgca gatagttcag cagcaaggga acgggacggc cagtcgcacc tttttccgct
     3901 ccggatttcc acgccgtacc cgcgatgtca aggtgcgccc acgggtagcc ttcggtgaag
     3961 taagacagga atgttgcggc ggtaatcgtg cccgcgccgg gcgtgccgat gtttggaatg
     4021 tcggcaaagt tggatttgag ttggtctttg taggtctcaa agagcggcag ttgccatgct
     4081 ttgtcgtcca cgttgtagga agcggcaagc aggctgtcga tcaaatcctg attgttgccc
     4141 atcacgccgc tgacatcgtg ccccaaggca acaatacacg cgccggtcag ggtggcgacg
     4201 tcgatgacgg ctttgggttt gaactgctcg gcgtaagtga gcgcgtcgca caaaatcaga
     4261 cggccttcgg catcggtgtt caacacttcg atggtcagac ctttcatact tttcacgaca
     4321 tcgcccggtt tgtttgccgc gccggaaggc atattttcac aagtggcgac gacggcaatc
     4381 aggttaatcg gcagttgcag tttgacggcg gcgcagaagg tgctgatgac ggttgccgcg
     4441 ccgcacatat caaacttcat ttcgtccatg ttcaggccgg gcttgaggga gatgccgccg
     4501 gtgtcgaagg taatgccttt gccgaccaat accacaggcg cggcttcttt gtcggctgca
     4561 ccgaaatagc tcagttcgac caaatagggg gcttccgcgc tgcctttggc gaccgaccaa
     4621 aacgaaccca tgttttcttt gatgtagtct ttttcgatga ttttggcgtg cgcgcccagt
     4681 ttttcggctt cggctttggc ggtgcgcgct aaaaattcgg gcgtgcattc gttgggcgcg
     4741 gcgttgccca agtcgcggca gaggctttgt ccgtaaactt gcgcttcggc gacgcgcaag
     4801 gcttctttaa cgacggcttc gtgtgcgctg tggaacacgg cggtttcaaa tttggcgggc
     4861 ttggcttctt ttttgtagcg gtcgaaacgg taggcggcgt tgccgaacgc aatcgcaaac
     4921 gcttcggcta cggcggcggc ttgcgcttca tcgaaggcat ggatgtccac attgaccgtt
     4981 tcctgatttt gcgcccattt ggcggcttcg gcggcggcct tgttcaatgc ggcgcggccg
     5041 gtgcttttca gacagcatac ggcaacagcc tgcaaaccgt tgcctgtcgg gatttttgtg
     5101 tcggcaaaat tttgaccttc ttcaagcgaa gacaaaaggg caagggcggt cggatggttt
     5161 aattgcgcct cttcggtgca gacaaataac tgtgcgcctg cctgctgttc ctgcaagatt
     5221 tcggtttttg tgctaaattc cacgtttatt ctcctgattg agacggttgt cggtagtttt
     5281 cggacggcct ttcgctcaaa agaccgtctg aagacggctg gcacgattgt accccatttg
     5341 aagcaccgtc tgaaaccttg cgcggacaat ccgcctgcgc cgaaccgctt accgccccct
     5401 gaccgcgatt ctatgattta tcaaagaaac ctcatcaaag aactctcttt taccgccgtc
     5461 ggcattttcg tcgtcctctt ggcggtattg gtctccacgc aggcaatcaa cctgctcggc
     5521 cgtgccgccg acgggcgtgt cgccatcgat gccgtgttgg cattggtcgg cttctgggtc
     5581 atcggtatga cgccgctttt gctggtgttg accgcattta tcagtacgtt gaccgtgttg
     5641 acccgctact ggcgcgacag cgaaatgtcg gtctggctat cctgcggatt ggcattgaaa
     5701 caatggatac gcccggtgat gcagtttgcc gtgccgtttg ccgttttggt tgccgtcatg
     5761 cagctttggg tgataccgtg ggcagagcta cgcagccgcg aatacgctga aatcctgaag
     5821 cagaagcagg aattgtcttt ggtggaggca ggcgggttca acagtttggg caagcgcaac
     5881 ggcagggttt attttgtcga aaccttcgat accgaatccg gcatcatgaa aaacctgttc
     5941 ctgcgcgaac aggacaaaaa cggcggcgac aacatcatct tcgccaaaga aggtaacttc
     6001 tcgctgaacg acaacaaacg cacgctcgaa ttgcgccacg gctaccgtta cagcggcacg
     6061 cccggacgcg ccgactacaa tcaggtttcc ttccaaaaac tcaacctgat tatcagcacc
     6121 acgccaaaac tcatcgaccc cgtttcccac cgccgtacca ttccgaccgc ccaactgatt
     6181 ggcagcagca acccgcaaca tcaggcggaa ttgatgtggc gcatctcgct gaccgtcagc
     6241 gtcctcctac tctgcctgct tgccgtgccg ctttcctatt tcaacccgcg cagcggacat
     6301 acctacaata tcttgattgc catcggtttg tttttaattt accaaaacgg gctgaccctg
     6361 ctttttgaag ccgtggaaga cggcaaaatc catttttggc tcggactgct gcctatgcac
     6421 atcatcatgt tcgtcatcgc aatcgtactt ctgcgcgtcc gcagcatgcc cagccagccc
     6481 ttctggcagg cggttggcaa aagtctgaca ttgaaaggcg gaaaatgaac ctgatttcac
     6541 gttacatcat ccgtcaaatg gcggttatgg cggtttacgc gctccttgcc ttcctcgctt
     6601 tgtacagctt ttttgaaatc ctgtacgaaa ccggcaacct cggcaaaggc agttacggca
     6661 tatgggaaat gctgggctac accgccctca aaatgcccgc ccgcgcctac gaactgatgc
     6721 ccctcgccgt ccttatcggc ggactggtct ctctcagcca gcttgccgcc ggcagcgaac
     6781 tgaccgtcat caaagccagc ggcatgagca ccaaaaagct gctgttgatt ctgtcgcagt
     6841 tcggttttat ttttgctatt gccaccgtcg cgctcggcga atgggttgcg cccacactga
     6901 gccaaaaagc cgaaaacatc aaagccgcgg ccatcaacgg caaaatcagt accggcaata
     6961 ccggcctttg gctgaaagaa aaaaacagca ttatcaatgt gcgcgaaatg ttgcccgacc
     7021 ataccctgct gggcattaaa atctgggccc gcaacgataa aaacgaactg gcagaggcag
     7081 tggaagccga ttccgccgtt ttgaacagcg acggcagttg gcagttgaaa aacatccgcc
     7141 gcagcacgct tggcgaagac aaagtcgagg tctctattgc ggctgaagaa aactggccga
     7201 tttccgtcaa acgcaacctg atggacgtat tgctcgtcaa acccgaccaa atgtccgtcg
     7261 gcgaactgac cacctacatc cgccacctcc aaaacaacag ccaaaacacc cgaatctacg
     7321 ccatcgcatg gtggcgcaaa ttggtttacc ccgccgcagc ctgggtgatg gcgctcgtcg
     7381 cctttgcctt taccccgcaa accacccgcc acggcaatat gggcttaaaa ctcttcggcg
     7441 gcatctgtct cggattgctg ttccaccttg ccggacggct cttcgggttt accagccaac
     7501 tctacggcat cccgcccttc ctcgccggcg cactacctac catagccttc gccttgctcg
     7561 ccgtttggct gatacgcaaa caggaaaaac gctaaaccaa tgccgtctga cccccttcgg
     7621 acggcattgg tttttattat catattcctg tagacagata gccgttccct attatagtgg
     7681 attaacaaaa accagtacgg cgttgcctcg ccttgccgta ctattgtact gtctgcggct
     7741 tcgtcgcctt gtcctgattt ttgttaatcc actatacatt acctgtcata acagttccat
     7801 ttttgttaaa actagtctat gatagcggta caaatattgt ttacaatatt taacgcaaat
     7861 catttgcaac ccgacaaaag aaaaacagaa aaaggaacaa agagatgtta gaagcctatc
     7921 gtaaagccgc cgccgagcgc gccgccctcg gcattcccgc cctccctttg aacgcgcagc
     7981 aaaccgccga tttggttgag ctgctgaaaa gcccgcccgc aggcgaaggc gagttcttgg
     8041 tcgaactgct tgcccaccgt gttccgcccg gtgtggacga tgccgccaaa gtcaaagcct
     8101 cattcctggc tgccgttgcc gaaggcagcg cgtccagccc gctgatctcc cccgaatatg
     8161 cgaccgaact cttaggtacg atgctcggcg gttacaatat ccacgcctta atcgaactct
     8221 tggacgacga caaactcgcg cccattgccg ccaaaggctt gaaacatacg cttctgatgt
     8281 tcgattcctt ccacgacgtt caggaaaaag ccgaaaaagg caacaaatac gcgcaagaag
     8341 ttttgcaatc ttgggcagat gccgaatggt tcgcctcacg cgccaaagtt cccgaaaaaa
     8401 tcaccgttac cgttttcaaa gttgacggcg aaaccaatac agacgacctc tcccccgcgc
     8461 ccgacgcgtg gagtcgtccc gatattccgc tgcacgcgct ggcaatgctg aaaaacccgc
     8521 gcgacggcat cacgcccgac aaaccgggcg aagtcggtcc gattaaattg ttggaagaac
     8581 tcaaagccaa aggccatccg gttgcttacg tcggcgacgt ggtcggtact ggttcttcac
     8641 gcaaatccgc gaccaactcc gtcatttggc ataccggcga agacattccg ttcgtgccga
     8701 acaaacgctt cggcggcgta tgtttgggcg gcaaaatcgc gccgattttc ttcaataccc
     8761 aagaagattc cggcgcgctg ccgattgaag tcgatgtatc tgctctaaaa atgggcgatg
     8821 tcgtcgatat cctgccttat gaaggcaaaa tcgtgaaaaa cggcgagact gttgccgagt
     8881 ttgaattgaa atcacaagta ttgctggacg aagtgcaagc cggcggccgt atcaacctga
     8941 ttatcggccg aggtctgacc gccaaagcgc gcgaagccct gaaactgcct gcctctactg
     9001 cattccgcct gccgcaagcg cctgccgaaa gcaaagccgg tttcaccttg gcgcaaaaaa
     9061 tggtcggccg cgcctgcggt ctgcccgaag gacaaggcgt gcgcccgggt acttactgcg
     9121 aaccgcgtat gacgacggtc ggctcgcaag acacgaccgg cccgatgacc cgcgacgagt
     9181 tgaaagactt ggcttgtttg ggcttctccg ccgatatggt gatgcagtct ttctgccaca
     9241 ccgccgccta tccgaaacct gtcgatgtaa aaacccataa agaactgccc gcctttattt
     9301 ccacccgtgg cggcgtgtca ctgcgtccgg gcgacggcgt catccactcg tggctcaacc
     9361 gcctgctgct gcccgatacc gtcggcaccg gcggcgacag ccatacccgt ttccccatcg
     9421 gtatttcctt ccccgccggc tccggcttgg ttgcctttgc cgccgcaacg ggcgtaatgc
     9481 cgctcgatat gcccgagtct gtattggtac gcttcagcgg caagctgcaa ccgggcgtaa
     9541 ccctgcgcga tttggtgaac gccatcccgc tgtacgcaat taaacaaggt ttgctgaccg
     9601 ttgccaaagc cggtaagaaa aacatcttct ctggccgcat cctcgaaatc gaaggcctgc
     9661 ccgatttgaa agtggaacaa gcctttgaat tgaccgacgc atccgccgaa cgctccgccg
     9721 ccggctgtac cgtgaagctc aataaagagc cgattatcga gtacatgaaa tccaacgtcg
     9781 tgttgatgaa aaacatgatt gccaacggct atcaagaccc gcgcactttg gaacgccgca
     9841 tcaaagccat ggaaaaatgg ctggcaaatc ccgagttgct cgaagcggat aaagatgccg
     9901 aatacgccgc cgtgattgaa atcaacatgg acgacatcaa agagccgatt atcgcctgcc
     9961 cgaacgaccc ggacgacgtg tgcttcatgt ccgaacgctc cggcaccaaa atcgacgaag
    10021 tattcatcgg ttcgtgtatg accaacatcg gccacttccg cgccgcctcc aaacttttgg
    10081 aaggcaaggc agacaccccc gtccgcctgt ggattgcgcc gccgaccaaa atggacgcga
    10141 aacaattgtc cgacgaagga cactacggcg tactcggacg tgccggcgcg cgtatggaaa
    10201 tgccgggttg ctccttatgt atgggtaatc aggcgcaagt acgcgaaggt gcgaccgtta
    10261 tgtccacctc cacccgcaac ttcccgaacc gtttgggtaa aaacaccttt gtttacctcg
    10321 gttcggcgga attggcagcg atttgctcca aactgggtaa aatcccgacc gttgaagaat
    10381 atcaagccaa tatcggcatc atcaacgaac agggcgataa aatctaccgc tatatgaact
    10441 tcaacgaaat cgacagctac aacgaagtag ccgagaccgt gaacgtttaa tccccgtcat
    10501 ccgtatgaag taagggattg accgcaatgc cgtctgaaca accttcagac ggcattgcaa
    10561 cattccgcta acccttcttt ccgcaaacgc tgcaaatacg gcgttcacgc ccccacataa
    10621 aggaaacgac agtgaacctg aaaaaccgcc attttctgaa acttttagac ttcacgccgg
    10681 aagaaatcac cgcctacctc gaccttgccg ccgaattgaa agccgccaaa aaagcagggc
    10741 gcgagattca gcggatgaaa gggaaaaaca tcgccctgat ttttgaaaaa acctctactc
    10801 ggacgcgctg cgcgtttgaa gtcgccgcgc gcgatcaagg cgcgggagtg acttatttag
    10861 agccgtccgc cagccaaatc gggcataagg aaagcatcaa agacaccgcc cgcgtgttgg
    10921 gcaggatgta cgatgccatc gaatatcgcg gtttcggtca ggaagttgtt gaagaattgg
    10981 cgaaatacgc gggcgtaccc gtgttcaacg ggctgaccaa cgagttccat cccacacaaa
    11041 tgcttgccga cgcactgact atgcgcgaac acagcggcaa acctttgaac caaaccgcgt
    11101 ttgcctacgt cggcgacgcg cgttacaaca tgggcaattc cctgctgatt ttaggggcaa
    11161 aattggggat ggacgtgcgt atcggcgcac cgcaaagcct gtggccgtct gaaggcatta
    11221 ttgccgccgc acacgccgcc gccaaagaaa ccggcgcaaa aattaccctg accgaaaacg
    11281 cgcatgaagc cgtgaagaat gttgatttta ttcataccga tgtgtgggtc agcatgggcg
    11341 agccgaaaga agtctggcag gaacgcatcg atttgctgaa agattaccgc gttacgcccg
    11401 aactgatggc ggcatcgggc aatccgcaag tcaaattcat gcactgcctg cccgccttcc
    11461 acaaccgcga aaccaaagtc ggcgaatgga tttacgaaac cttcgggctg aacggtgtgg
    11521 aagttacaga agaagtattc gaaagccccg ccagcatcgt gttcgatcag gcggaaaacc
    11581 gtatgcacac gattaaagcg gtaatggtcg cggctttggg cgactgacag aactgtgcct
    11641 gtttaaattc atccgcaaca cagataccgt ctgaacacga tgttcagacg gtatcca
//
LOCUS       DAWFAN010000053        11611 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWFAN010000053 DAWFAN010000000
VERSION     DAWFAN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.053"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(174..866)
                     /locus_tag="WGO65_001004"
     CDS             complement(174..866)
                     /locus_tag="WGO65_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0327308.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGAPKYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(974..2167)
                     /locus_tag="WGO65_001005"
     CDS             complement(974..2167)
                     /locus_tag="WGO65_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0327309.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2296..3033)
                     /gene="pxpA"
                     /locus_tag="WGO65_001006"
     CDS             complement(2296..3033)
                     /gene="pxpA"
                     /locus_tag="WGO65_001006"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ0327310.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3020..3949)
                     /locus_tag="WGO65_001007"
     CDS             complement(3020..3949)
                     /locus_tag="WGO65_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ0327311.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3946..4593)
                     /gene="pxpB"
                     /locus_tag="WGO65_001008"
     CDS             complement(3946..4593)
                     /gene="pxpB"
                     /locus_tag="WGO65_001008"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ0327312.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4777..6984
                     /gene="uvrD"
                     /locus_tag="WGO65_001009"
     CDS             4777..6984
                     /gene="uvrD"
                     /locus_tag="WGO65_001009"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ0327313.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQMPLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNATLESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7341..7880
                     /locus_tag="WGO65_001010"
     CDS             7341..7880
                     /locus_tag="WGO65_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0327314.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8070..8285)
                     /locus_tag="WGO65_001011"
     CDS             complement(8070..8285)
                     /locus_tag="WGO65_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327315.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8293..8496)
                     /locus_tag="WGO65_001012"
     CDS             complement(8293..8496)
                     /locus_tag="WGO65_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327316.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8409..8768)
                     /locus_tag="WGO65_001013"
     CDS             complement(8409..8768)
                     /locus_tag="WGO65_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0327317.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8821..9198
                     /locus_tag="WGO65_001014"
     CDS             8821..9198
                     /locus_tag="WGO65_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0327318.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVQISILKQLVKSSLS"
     gene            9397..10017
                     /locus_tag="WGO65_001015"
     CDS             9397..10017
                     /locus_tag="WGO65_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0327319.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            10040..11491
                     /locus_tag="WGO65_001016"
     CDS             10040..11491
                     /locus_tag="WGO65_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0327320.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSIAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT         2852 a         3076 c         2836 g         2847 t
ORIGIN      
        1 ctctttcagt tacagcagct tgatcccttt cccttatcca acgggggaag gctaggatag
       61 ggtggcttgc aaatatacag aacaagggac aagagccacc ctctctccaa ccctctccct
      121 ccgtacggga gggggtggat tctcgcgggc gaagcccacg ctacggttag cctttacccc
      181 agcacaaaca attcccgccc gtgcgccttc agccaacttt tagcattgtc ggtatgcggc
      241 gtcagcgtgt tcaccaaatg ccaaaagcgc ggactgtggt cggggtggcg gaggtggcag
      301 agttcgtgga tgcagacata gtcggcaacg tacttgggcg cgccgatcag ccgccagttg
      361 aggcggatgc cggtgtgcgg gcggcatacg ccccaaaagg ttttggcgtt gctcaggtct
      421 gtggcggtgg gcgtcagtcc tgtttcggct gcgtgttttt caaggcgggg cagcaggtat
      481 tcgcgggcgc gttcgttcaa caggcggcgc aggtggtcga tttgtgcggc ggtttctttt
      541 cggggaagca ggatttcaga cgacgtgata cggatatggc tttggctgtg ggtatccagc
      601 ttggtcttta ttccccgata ccaaatccac tcgggtaagt ttgggtggga aacaggatgc
      661 acgggcgttt tggcaagcgt gttccgcaaa atcgtttcgt ttgccgccag ccagtttgct
      721 aacgcgtggt cttgaaaaaa gggtgggacg ttgatgctga ccgtctgcat attgacgggg
      781 cgcagaatca gatttttctt ggcactgcgt ttgagttcga tttcgatgca caaaccgtcg
      841 gaaagagtat aggtgaagcg tttcatagtt gtgaataggt ttcagaccgg atacatcgtc
      901 tgaaacagga attttccata tcaggcggca aacttcggat aatatacaaa atcaaacatc
      961 tgcgctacaa ggttcagccg aacaagccgc cgatatattt gctgatggta atggcgctga
     1021 gtactgccat caaaccgacc acaatcacgc cggaaacggt gagccacagc gggtgtttgt
     1081 agtcgccgac aattttggtt ttgtaggcgg caatcagaat cagaccgagg gaaatcggta
     1141 aaatcaggcc gtttaatgcg cctacgaaca ccagcacctg cgccggtttg ccgatggtgg
     1201 aaaatacggc ggtggacacg gcgataaagg caataatcca tttgttttta ttgcgttcga
     1261 tagacgggct gagaccggag aagaacgaca ccgaagtata agccgcacca atcaccgaag
     1321 taatcgaagc cgcccaaatc accacgccga aaatcagcag gccgatgtat cccgccgcat
     1381 attcaaacgg tgtggaagca gggttgtcgg gattgagctg tacgccttgg ctgaccacgc
     1441 ccaaaaccgc caaaaacaat acaatccgca taatcgaggc aatcaggatc gcccgcaccg
     1501 agctttggct cacttccggc aacgccgatt tgcctttgat gcctgcgtcc agcagacggt
     1561 gcgcaccggc gaaggtgatg tagccgccga ccgtgccgcc caccagtgta acaatcgcca
     1621 ttgcatcgag tttttccggc ataaaggtat gcacggcggc atctgccagc ggcggattcg
     1681 cctgccatgc cacataaacc gtcagcgcaa tcattacgaa acccatcact tgggcgaatt
     1741 tgtccatcac tttgcctgct tctttaaaca gaaacacacc gatggcaatc acgccgctga
     1801 tcacggcacc ggtttccggc gacagtccgg tcagcaggtt cagacccaag cctgcgccgc
     1861 cgacgttgcc aatattgaac gccaaaccgc ccatcacaat cagcacagcc aagaaatagc
     1921 ctgcgccggg caagacctga ttggcaatat cctgcgcctg tttttcggaa acggcgacaa
     1981 tccgccaaat attgagctgc gccccgatgt cgagcagaat cgagagcaga atcacaaagc
     2041 cgaaacttgc cgccagtgct tgggtgaagg tggcggtttg ggtcagaaag cccgggccga
     2101 tggcggaagt cgccatcagg aatgcagcgc cgattaaggc atttctgcgg tttttttgat
     2161 tagacataat cgcttatcct ctataaaatt ggttgttgct gtgtttgggc gaaacctgcg
     2221 gttttagcta cgcagaaact cgctttgctc gttttggcga aacctgcggt tttcagacgg
     2281 cctatgaact gtttttcaag cagaaacttt gatgcctgcc gccagtagtt cctgccggat
     2341 tttttcggca aacaccacgg cgtgcggccc gtctccgtgc agacagatgc tgtcggcttg
     2401 cacggcaacc aggctgccgt tcactgcttt gacccgcccg tcccgcacca tctgcaatac
     2461 ttgggcgatg gcttcttcgt cgctgtccac ctgcgcatcg gggcggctgc ggggaaccag
     2521 cgtaccgtcg ggcatatagc ggcggtcggc gaatacttcg gaaatcacac ccaagcctgc
     2581 ggcttttccg gcttccaaga gcaggctgcc ggaaagtgcc atcaatttca atttcgggtc
     2641 gaaatccgcc acaattcggg caacggtatc cgccagcgca cggtttttcg ccgcttggtt
     2701 gtacattgcg ccgtgcggtt tgacataagc catttccaaa ccctgatcac ggcacaaggc
     2761 ctgcaatgcg cccaactggt aattcagaca cgcccgcaaa tcggcttcgg acagattcat
     2821 ttcggtacgg ccgaagtttt cccgatcggg atagccgggg tgtgctccga tgcgcacgcc
     2881 gttttgttgg gcatacgcca atgccgcccg aatatcggca atgctgccgg cgtgttgggc
     2941 gcaggcgatg ttggccgaag taatcagctg caacaaggct tcgtcgctgc cgcagccttc
     3001 ggcgagatcg gcgtttaaat caacctgctt catgggtgat tctccgtatt tggttcagat
     3061 aggcttggtt tttgcgccgc agggcggtgg cttctttcaa gccgattatt ttgaatttga
     3121 ctttgctgcc gaagcgcacc tgtgccagcc tgcccaaatc ggcggcggca acggtagcga
     3181 ttttcggata accgccggtg gtttgtgcat cggccagcag gataatcggt ttgccgccgg
     3241 gcggcacctg cacggttcct gcctgaacag cgtgggacag catttccaaa ggttgcgaca
     3301 gggtcagcgg ctgtccgtcg aagcggtagc ccatgcggtt gctatcgctt tgcagcgtcc
     3361 acgtttcccg ttccagattc agacgccctt tttcactgaa agcggcatat tccgacgaag
     3421 gaacaaggtg gacggtatcg gtaaacggta tcggggcaat gccgactttg gacaattcct
     3481 gcgcaccttt gccgatgggg agacaatcac ctttttgcag cattctgccc tgatggccgc
     3541 cgaaaccggc tttcaggtcg gtgcttctcg aacccatcac ttccggcaca tcaaatccgc
     3601 ccgccacgca cacatagccg tacatgccct gcacggcacg caccattttc aaggtctgcc
     3661 ctttgcgggc ggtataacgc caatacgaat agaccggttc gccgtccaat tccgcctgat
     3721 acacggcacc ggtgagacaa aacggcgtat cccgttcaaa caccagcatt atcccgccca
     3781 aagcgatttc gattgcggcc gtgccttcgt cgttgcccaa taaaatatta cccgccgcca
     3841 aagcaaccgt gtccatcgca ccggcatgac cgatgccgta acgccggtgt ccgtagcgtc
     3901 cggtatcctg aatatgcgcc ggtgcctgca ctgccgaaac gtgaatcatg gctcaatcct
     3961 ttctgcaaca aagcggactt ggtcacccgc cgccagcagg gtcggcggat tcaaatcggc
     4021 tcggaacaag ggtaattcgg ttctgccgat aatctgccag ccgccgggcg aagcgaacgg
     4081 atacacaccg gtctgactgc cgccgatacc gaccgaaccg gcaggaacgg acgttctcgg
     4141 cacggcacgg cggggcgtgt gcaatgcttc gggcaagccg cccagataag ggaaaccggg
     4201 ctggaagccc atcataaata cggtataagt ttgcgccgta tggcggcgga cgatttcgga
     4261 aataaccgtc tgatggaaag cagcgacttc cgccaaatcc gggccgtatt cgccgccgta
     4321 gcagacggga atttccacca gtttgccctg atggtctgta acggcggtgt gttcccacac
     4381 atattgcaat tcatcggcaa gcgtcgccaa atcggtatcg aaacgggtga acacggtcag
     4441 attgttcatg ccgaccacca cttcctcaat cctgtcgtgc tgcccgagcg cagcggcaaa
     4501 cgcccacaac ttttgctgtt tgcccagttc ggaaggcgca ttcagtcggc agaccaaagc
     4561 ggattcgctg attggtgtga tctctattct catttgttgt tcattttggt tatgttttaa
     4621 tgaatctata tgcaggggcg gcggtttgtc aatatcttct gtgctgcatc atcaaaccgt
     4681 cgattggaaa agtgctgccc tgccgctgca ctttttcaga cgaccttaaa ccgtttctat
     4741 taaaatagcg cattccactt ttcagacggc atccttatgt ttcccgacca atccgccccc
     4801 aacctgctgc aaggcttgaa tcccgaacaa ctctccgccg taacctggcc gccgcaatcc
     4861 gcccttgtgc tggcgggcgc gggcagcggc aaaacgcgcg tgctgaccac gcgcatcgca
     4921 tggctgttgc aaaccggtca ggcaagcgtg cacagcatta tggcggtaac gtttaccaac
     4981 aaagccgcca aagaaatgca aacccgactc ggcgcgatga ttcccatcaa cgtccgcgcc
     5041 atgtggctcg gcacgttcca cggtctctgc caccgctttt tgcgcctgca ccaccgcgac
     5101 gccggtctgc cgtcttcctt tcaaatcctc gacggcggcg accagctttc cctcatcaaa
     5161 cgcctgctca aaagcctcaa catcgccgaa gaaatcatcg cgccgcgttc gctgcaaggc
     5221 tttatcaacg cgcaaaaaga atccggcttg cgcgcttccg tcttgagcgc gcccgacccg
     5281 cacacaagcc gcatgatcga atgctacgcc gaatacgaca aaatctgcca acgcgaaggc
     5341 gtggtcgatt ttgccgaact catgctccgc agctacgaaa tgctgcaaag caacgaaatc
     5401 ctgcgccaac actaccaaaa ccgcttcaat cacattctgg tcgacgaatt ccaagacacc
     5461 aacaaactgc aatacgcctg gctcaaactc atggcgggcg gcaacgcggc ggtatttgcc
     5521 gtcggcgacg acgaccaaag catctaccgt ttccgtggcg caaacgttgg caatatgacc
     5581 gcgctgatgg aagaattcca catcgacgcg cccgtcaaac tcgaacaaaa ctaccgctcc
     5641 gtcggcaaca tccttgccgc cgccaatgcc gttatcgaaa acaacgacga acgactcggc
     5701 aaaaacctgc gcaccgacgc cgaagcaggc gacaaaattc gctactactc cgcctttacc
     5761 gacctcgaag aagcccggtt catcgtggac gaaaccaaag ccctcgaacg cgaaggctgg
     5821 gatttggacg aaatcgccgt cctctaccgt agcaacgccc aatcccgcgt tatcgaacaa
     5881 agcctgttcc gcagcggcat cccctacaaa atctacggcg gcttgcgctt ttacgaacgc
     5941 caagaaatca aacacgccct cgcctacctg cgcctcgccg tcaatcccga cgacgacaac
     6001 gccctcttgc gtgtcatcaa cttcccaccg cgcggcatcg gtgcacgtac cgtcgaaaat
     6061 cttcagacgg cctcaaacga acaaggcatc accctctggc aagccgcctg caacgccggc
     6121 gcgaaagccg ccaaagtcgt cgccttcgtc cgcctgattg aagccctgcg caaccaagtc
     6181 ggacaaatgc ccctgtccga aatcatcgtc ggcatcctca aagacagtgg cttgaccgaa
     6241 cactaccgca cccaaaaagg cgacaaccaa gaccgcctcg acaaccttga cgaactcgtc
     6301 aacgccgcca tcgagttcaa accagaagac agcaactttg aaaccctgcc tgaaaacatt
     6361 tcagacgatc ccgccttccc catcctcgct ttcttgagca acgccaccct cgaatccggc
     6421 gaaaatcaag caggtgcagg cgaaaaagcc gtccaactca tgaccgtcca cgccgccaaa
     6481 ggcttggaat ttaacgccgt cttcctcacc ggcatggaag aaggccgctt ccccagcgaa
     6541 atgagtctcg ccgaacgcgg cggcctcgaa gaagaacgcc gcctcatgta cgtcgccatc
     6601 acccgcgccc gcaaacgcct ctacatcacc atggcgcaac aacgcatgct gcacggacaa
     6661 acccaattcg gcatcgtctc ccgcttcgtc gaagagatcc cacccgaagt attgcactac
     6721 ctgtccgtca aaaagaccgc ctacgacagc tacggcagcc cgcgccaaac cgccgcatcc
     6781 aaagacaaaa tcatcgacga ctacaaacag ccccaaacct acgcaggctt ccgtatcgga
     6841 caaaacgtcc gccacgccaa attcggcacc ggcgtgatta tcgatgccgc agataaaggc
     6901 gaatccgccc gactgaccat caacttcggc aaacagggcg tgaaagagtt ggataccaag
     6961 tttgcgaaat tggaagcgat gtaaatttga aatgcaggct ggatactcgt atccgacaaa
     7021 aatatttgac gcgtctatcg tttccgaaaa accgctgttg gaaaatgtcg gattcgagaa
     7081 tccgacctat ggcaaaaaac tgtagcaagg acaaggcaaa aggccgtctg aatccggtaa
     7141 caacaaattt tgccgaaacc tccgccgtca ttcccgcgca ggcgggaatc cagacctttc
     7201 ggcacgggaa cttatcggat aaaaggtttc tttagattcc acgttctaga ttcccgcctg
     7261 cgcgggaatg acggacggta aaagccgggt atagatacct aacccttgtt tatagctgaa
     7321 aatcaataag gaagaacatt atgtcccaag tttttaaaga ttttgatttg tcctccgtat
     7381 gggaatccga cagttgggca gatgaaaact acaaagaagc cccgtttacc cctgaaattt
     7441 tggctgccgt agaaagtgaa ctgggctata aattgccgca aagttttatt gaattgatgg
     7501 cggtacaaaa cggcggaata tttgtcaaaa actgttttcc gaccacacag agaaattcgt
     7561 gggcgaaaga tcatgtgcaa atttgcgagg tatcgggcat cggctttgaa aaagaaggga
     7621 gtttgtgcgg cgaaacaggg caaaaacttt ggatggaaga atgggaatat ccgcccatcg
     7681 gcgtgtattt tgccaacgac ccgtcaggcg gtcatgccat gtttgccttg gactatcggg
     7741 agtgtggaaa agacggcgag ccgaaagtgg tgtttgtcga gcaagaatcg gattttgaaa
     7801 tcgtcgaact tgcccccgat tttgaaacct ttatccgcag cttgcggcat gaagatgagt
     7861 ttattgacga ggaaatataa aacggcggtt gaaaaaccgg aatcatcaag ggaaaacggg
     7921 cgaaataacg gataatcgct tgaatccgta aggaaaacgg tttggtggaa cgcgccatcc
     7981 aagacctttg caaagaaaaa ctgtctccga cagcattgac attattaaca gaacttatca
     8041 agtttggagc tatctcaaat ataattcggt tatccttttg tatccattaa atcatatgct
     8101 tcaattaatt gttgttctag ctcttgtacc aattttgaat tgcgaattcc tgacacaatc
     8161 tcaaattctt ctgcatctat gcaaacacct gcataaattt caataacaag ggaacgcaat
     8221 aattgaagct cttctcttgt taaagaaata ataatgtcat cacctgtgta attgattata
     8281 ttcataataa ttttattttt gtttgtcaaa gtaagttttg cctaaggttg gtctaaatgc
     8341 agttccacca tcttttgaat ttgggtctct gattacaatt gctccagact tatcatccca
     8401 aattgctctt atgtgtttgg attgtaatct tcgaattccc aagaaaaaaa tcgtaataag
     8461 tttgaaagtg tcaaatccca agtttctttt gagcaatatt ctaatatttt atcaatttca
     8521 cttttaataa tcttatgatc aaactgttct aatattaatg cattagacca aaaaaaacct
     8581 tctttattac aatgatggga aatccattta ggagaacaaa tgcaaagtga aaaaatagat
     8641 gagccttgtt ctccttcgat tccgatatcc aaatctatcc atctatggaa attatctgga
     8701 atttcggggg taaatttttc aaaatcaata tcatataaat ttatgctttt taaatccaat
     8761 ttaatcatta gggctgtcct agataaatag ggaaattcaa attaagttag aattatccct
     8821 atgagaaaaa gtcgtctaag ccggtataaa caaaataaac tcattgagct atttgtcgca
     8881 ggtgtaactg caagaacagc aacagagcct gacagcattg tttatacgga ttgttatcgt
     8941 agctatgatg tattagatgt gagcgaattt agccatttcc gtatcaatca cagcacacat
     9001 tttgctgaac gacaaaacca tatcaatgga attgagaatt tttggaaccg ggcaaaacgt
     9061 catttacgca agtttaacgg cattcccaaa gcgcattttg agctgtattt aaaggagtgc
     9121 gaatggcgtt ttaacaacag tgagataaaa gttcaaattt ccattttaaa acaattagta
     9181 aaatcgagtt tatcttagtt gtccaggaca gccccattat ttttataaca ccgtgaagcc
     9241 gcacagcagt ttgaacagtg atacgccgtt tgcgggctta cgagtttatt ttcccggcct
     9301 gcagtttgag caatacggtg atttcctacg gttaatacaa atgtttacac attgatacat
     9361 ttcatttata gttccgccta tttgaaaata gaaaatatga attcgaccgc aagtaaaacc
     9421 ctgaaaggat tgtcgctggt gtttttcgcc tctggattct gcgccctgat ttaccaggtc
     9481 agctggcaga ggcttctatt cagtcacata ggtatcgatt tgagttcgat tactgtcatt
     9541 atttctgtat ttatggtcgg cttgggtgta ggtgcgtatt tcggtggacg cattgctgac
     9601 cgttttcctt caagtatcat ccccctgttt tgcatcgctg aagtatccat cggtctgttc
     9661 ggtttggtaa gcaggggtct gatttccggc ttggggcatc ttttagttga ggctgatttg
     9721 cccatcatcg ctgctgccaa tttcctctta ttgctgcttc ctacctttat gatgggcgcg
     9781 accttgccct tgctgacctg tttttttaac cggaaaatac ataatgttgg cgagtctatc
     9841 ggtaccttat attttttcaa cactttgggt gcggcactcg gatcgcttgc cgccgccgaa
     9901 tttttctacg tcttttttac cctctcccaa accattgcgc tgacagcctg ctttaacctt
     9961 ctgattgctg cttcagtatg gctgcgttac agaaaggatg gatatagtga acactaaacc
    10021 gaatactagt ttgatttata tgctttcttt ccttagcggc ttattgagct tgggtataga
    10081 agtcttgtgg gtaaggatgt tttcgttcgc agcacagtcc gtgcctcagg cattttcatt
    10141 tactcttgcc tgttttctga ccggtatcgc cgtcggcgcg tattttggca aacggatttg
    10201 ccgcagccgc tttgttgata ttccctttat cgggcagtgc ttcttgtggg cgggtattgc
    10261 cgactttttg attttgggtg ctgcgtggtt gttgacgggt ttttccggct tcgtccacca
    10321 cgccggtatc ttcattaccc tgtctgccgt cgtcagaggg ttgattttcc cgctcgtaca
    10381 ccatgtgggt acggatggca acaaatccgg acgacaggtt tccaatgttt atttcgccaa
    10441 cgttgccggc agtgcattgg gtccggtcct tatcggcttt gtgatacttg atttcttgtc
    10501 cacccaacag atttacctgc tcatctgttt gatttctgct gctgtccctt tgttttgtac
    10561 actgttccaa aaaagtctcc gactgaatgc agtgtcgata gcagtttccc taatgttcgg
    10621 catcctcatg ttcctactgc cggattctgt ctttcaaaat attgctgacc gtccggatag
    10681 gctgattgaa aacaaacacg gcattgttgc ggtttaccat agagatggtg ataaggttgt
    10741 ttatggggcg aatgtatacg acggcgcata caataccgat gtattcaata gtgtcaacgg
    10801 catcgaacgt gcctatctgc taccctccct gaagtctggc atacgccgca ttttcgtcgt
    10861 tggattgagt acaggttcgt gggcgcgcgt cttgtctgcc attccggaaa tgcagtcgat
    10921 gattgttgcg gaaatcaatc cggcataccg tagccttatc gcggacgagc cgcaaatcgc
    10981 cccgcttttg caggacaaac gtgttgaaat tgtattggat gacggtagga aatggctgcg
    11041 tcgccatcct gatgaaaaat tcgacctgat tttgatgaat acgacttggt actggcgtgc
    11101 ctattccacc aacctgttga gtgcggaatt tttaaaacag gtgcaaagcc accttacccc
    11161 ggatggtatt gtaatgttta ataccacgca cagcccgcat gcttttgcta ccgccgtaca
    11221 cagtattccc tatgcatacc gctatgggca tatggtagtc ggctcggcaa ccccggtagt
    11281 tttccctaat aaagaactgc tcaagcaacg tctctcccgg ttgatttggc cggaaagcgg
    11341 caggcacgta tttgacagca gcaccgtgga tgctgcagca caaaaggttg tctctcgtat
    11401 gctgattcag atgacggaac cttcggctgg ggcggaagtc attaccgacg ataatatgat
    11461 tgtagaatac aaatacggca gagggattta accgtcttaa agggtttcag gcaacgcagg
    11521 ttttaggtaa cgtcctgcta gttcaaaaaa accgcatcac agcagtcggg acaaaatggt
    11581 ttaaacattt tgtcccgaat tcttattcct a
//
LOCUS       DAWFAN010000054        11587 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWFAN010000054 DAWFAN010000000
VERSION     DAWFAN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.054"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(85..954)
                     /locus_tag="WGO65_001017"
     CDS             complement(85..954)
                     /locus_tag="WGO65_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0327321.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WGO65_001018"
     CDS             complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WGO65_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ0327322.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(1963..3060)
                     /locus_tag="WGO65_001019"
     CDS             complement(1963..3060)
                     /locus_tag="WGO65_001019"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ0327323.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WGO65_001020"
     CDS             complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WGO65_001020"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ0327324.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(4392..5291)
                     /locus_tag="WGO65_001021"
     CDS             complement(4392..5291)
                     /locus_tag="WGO65_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ0327325.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(5395..6048)
                     /locus_tag="WGO65_001022"
     CDS             complement(5395..6048)
                     /locus_tag="WGO65_001022"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ0327326.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(6127..6780)
                     /locus_tag="WGO65_001023"
     CDS             complement(6127..6780)
                     /locus_tag="WGO65_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ0327327.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            7057..7141
                     /locus_tag="WGO65_001024"
     tRNA            7057..7141
                     /locus_tag="WGO65_001024"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7091..7093,aa:Leu,seq:caa)
     gene            7850..9577
                     /gene="ilvB"
                     /locus_tag="WGO65_001025"
     CDS             7850..9577
                     /gene="ilvB"
                     /locus_tag="WGO65_001025"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ0327328.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDSLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
     gene            9588..10079
                     /gene="ilvN"
                     /locus_tag="WGO65_001026"
     CDS             9588..10079
                     /gene="ilvN"
                     /locus_tag="WGO65_001026"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ0327329.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            10146..10439
                     /locus_tag="WGO65_001027"
     CDS             10146..10439
                     /locus_tag="WGO65_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ0327330.1"
                     /translation="MSNIKIVALVTVKPEYTETLVAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            10534..11547
                     /gene="ilvC"
                     /locus_tag="WGO65_001028"
     CDS             10534..11547
                     /gene="ilvC"
                     /locus_tag="WGO65_001028"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ0327331.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRTDLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         2616 a         2764 c         3218 g         2989 t
ORIGIN      
        1 cataatcggg actgatttta agcgtggggt gataaaaatg ccgtctgaaa tatgttcaga
       61 cggcattgtg tttggaaaag gcaatcagtg ttctgccaat ttcaggtaaa cgccggaaac
      121 gtcctgttcg ccgtagccgg cttcgactgc tttgcggtag ctggcagcaa cggtttcgac
      181 ggcgggcagg gtgttgcctg cctgttcaag ctctttgacg gcgaggttga ggtctttgga
      241 ggcgtgtttg agggcgaagg cgggtgggaa ttcgcggttt gcccacaggg attttttggt
      301 ttggaacatg ggcgagtcca ttgccgagcc gccgatggct tcgacgatgg tgtcggtatc
      361 gatgccgaac tgccgcgcca tcagcatggc ttcgctgtaa gcttcgccga aaatgcccaa
      421 gagcgagttc aagacgagtt tcgcgcccga acctttgccg acatcgccga aatggaaggt
      481 ttttttgccg acgagggaaa atattttttg cagcgggttt aaaacggctt cgctgccgcc
      541 gaacagaatc agcagcgtgc cgttggtggc gggcccgacc gatccggaaa cgggtgcttc
      601 ggcaaactgt ccgcctgcgg cttcgacaag tgctttgacg gcgaggtttt cggtcgggga
      661 gatggtgctc atgttgacga tgattttgcc ggccaatccg tcgcggactc cgttcaggat
      721 gtcgcacacg gcggcatagt cggaaaccat caggaaaatg acgggatagt cgcggacgag
      781 ttcggcggtg ttgccgtaaa cttttgcgcc tttggcggag atgggggcag ttttgtcggg
      841 cgagcggttg tatacgccga cttcgatgcc gccgtccaag agccgcgtta ccataggcag
      901 acccatttgc cctaagccta tccagccgat ttgtgtgtat tcgtttgcgg acatgatgtt
      961 tactcctttt ctgtttgtag tgtagtgtaa tcttatctgt tccgataccg tctgaaaagc
     1021 ctgcgggtgt ttcagacggt atggtttttt atgcggtcag cgtgcctttg gtcgaggggg
     1081 tctgtcctgc catgcgcggg tcgtgttcga ctgccatacg cagggcgcgc ccgaaggctt
     1141 tgaatacggt ttccgcctga tggtgggcgt ttttgccgct gaggttgtcg atgtgcaggg
     1201 tcatcatact gtggttgacg atgccgtgga aaaattcttc aaacaaatcg acatcgaaac
     1261 gtccgattag tgcgcgggta aattcgatgt tgtacacgag tccggggcgg ccggaaaggt
     1321 cgatgacgac gcggctgagg gcttcgtcga gcgggacgta ggaatgtccg taacggcgga
     1381 tgcctttttt gtcgccgagt gcctgccgga ttgcttgtcc gagtgtgatg ccgatgtctt
     1441 cggcggtgtg gtggtcgtcg atgtgcaggt cgcctttgca gctgatgtcg atgtcaatca
     1501 tgccgtggcg ggcgatttga tcgatcatgt gttcgaggaa gggtacgccg gtatccagcc
     1561 tgcttttgcc gctgccgtcg aggttgatgg agacggtgat ttgggtttcg caggtattgc
     1621 ggttgacggt aacgctgcgg cagccggcag cagatgcggt ttcggcaatt tgggtttcgg
     1681 cagttccggt gctttctgcg gcggtttcgg ggacagtgcg ttcgcggtgt tttctgtcga
     1741 gccagccttt gggtttgccg gtgtgttttt cgagttttgc catcaggctg ggacggatgc
     1801 cgcgtgcatc tgggtcttct gcctgctttt caaggcgttg tttgagtttg tagagtgcga
     1861 cgggggtacg gtagccgcag agtttggcga gcttggacag cgaacctgct tcttgggcga
     1921 gggtcagaaa gttgtgcagt tggcgttgtg ttttagtcaa attcatagga aatccgttgg
     1981 ttggcagtaa agttggcgaa tgatgttgag aacggcatcg ttttgtgcgg ggctgcctac
     2041 ggtaatgcgc aggcagtgtt ccaaaagcgg gtgcgcgcca tgcagttttt taaccaagat
     2101 gcggttttgt ttgagcgtgt caaacaacaa atcggcatcg ggtacgcgta tggtaatgaa
     2161 gtttgcctga cttggaaagg tgttcagacg gcatattttg cccaattcgg cgaacatccg
     2221 ttcgcgttcg tttttcaggc tgtcgatgtt ggcagagatg atgccgtagt gttgcagggc
     2281 gagtttggcg gtggtcaggc tcagttggtt catattgtag ggcggcagga ttttttgcag
     2341 ttcgccgatg acttcgggac agcctgccgc atagccgata cgcagtccgg caaaaccgat
     2401 tttgctgacg gtacgcatga cgatgaggtt gggaatgctg cccgcctgcg gcaggaagct
     2461 gtcgccgttg aatgcgccgt aggcttcgtc gacgacgacg atgccgtctg aagcttcgat
     2521 gacggcttcg atttcggcac gcgtgaaaca tacgccggtg gggttgttgg ggtaggcgat
     2581 aaaggtcagg gcagggcggt gcttcctgac ggcttcgagg acggcgggca ggttgagggt
     2641 gaaatctccg ttcagtggaa cgccgacata atccatgccg tacagcgcgg cgttgtggcg
     2701 gtacatgacg aaactgggtt cggctgccaa cattgccgcg cccggtttgg cggtcagcat
     2761 cgtgatgaac tgtatcagct cgtccgagcc gttgcccagc gcgatgtcgg cgcagtcggg
     2821 aatgtcgaac gccgaacgta atgcttcctg taaaccgctg ccggagggat tggggtaaag
     2881 atggatgggc gcggcggcaa gccgtgcctg ccattcctgc aacagcgttt catgtccggc
     2941 aaaagggtgg acgggacttt ccatcgaatc gagtttggca aagccgggcg gaacgtcggc
     3001 aatctgatat gccgacatag cttgtatgtc gtcgcggatg aaggagcgga cggatttcat
     3061 cgtgtttcct taatggttgg aatatgcctg tacgccgttt cggcattatt tcatacggaa
     3121 ctctgccgcg cgggcgtggg cggtcaggct ttcgccgtgt gccagcacgc tggcggtttc
     3181 gcctaatttt tgcgcgccct gttccgaaac ctgaatcagg ctggagcgtt tttggaaatc
     3241 atatgtcccc aaaggcgagg aaaagcgggc ggttcggctg gtgggcaata catggtttgg
     3301 accggcgcag tagtcgccga ggctttcgcc ggtgtagcgt cccatgaaaa tcgcaccggc
     3361 gtggcggatt tttttcgccc attcctgcgg attttcgact gacagttcca agtgttcggg
     3421 ggaaatgtag ttggcgattt cgcaggcttc gtccaagtct ttggcgagta tcatcgcgcc
     3481 cctgttgccg agcgaggctt cgatgatgtc gcggcgcggc atagtttcga tcaggcggtc
     3541 catagcggct tctacttcgt cgagatacgc ttgcgacgtg ccgatgagga tggcttgggc
     3601 aatttcgtcg tgttcggcct ggctgaacaa atccatcgcc acccaatcgg caggtgtcgt
     3661 gccgtcggcg atgaccagga tttcagacgg ccccgccacc atgtcgatgc cgaccacgcc
     3721 gaacacgcgg cgtttggcgg cggcgacgaa ggcgttgccc ggaccggtga ttttatcgac
     3781 ttgggggatg gtttccgtgc cgtaggcgag ggcggcaacc gcctgcgcgc cgccgacggt
     3841 gaagactttg gttacgccgg cgacgtatgc ggcggcaagt acgatgtcgt tgcgttcgcc
     3901 ttttggtgtc ggcacgacca tgatgatttc tttcacacct gcgacgtggg cgggcatggc
     3961 gttcatgatg acggaactcg gatacgccgc cttgccgccg gggacgtaaa tgccgacgcg
     4021 gtcaagcggt gtgatttgtt gtcccaacag cgtgccgtct tcatcggtgt agctccacga
     4081 ttccattttt tggcgttggt ggtagctttc gacacggcgg gcggcggtct gcaatgccgt
     4141 ctgaacgtcg ttcggaatgc gctcgaacgc cgcgttcaaa tcggcttgcg tgagtattaa
     4201 atcatcgatg cttttagcgt ttgtctgatc gaatttgttg gtgtattcaa tcaaagccgc
     4261 atcgccgcgc ttttgcacgt cggcgcaaat gtcggcgacg atgcgttcgg tttcggggtt
     4321 ttgcgcggtt tcaaaagcca gcagggcttt gagtccggct tggaaatcgg gcgattgggt
     4381 gttgagtttt ttcatgattt ggttttttcc ttttttattt ttggattggg ggtcgtctga
     4441 atattctctt gttcagtttc tgtctctgtt ttgggtctga tatacgcttt ttctcccccg
     4501 ctaccaaact tcaattcggg caggcatttt tgttgcagac cgagtttggc taatacctct
     4561 tcgctacgag aggtagtgcg gaaacggtaa cgggattgtt ttctaccgtt tttggtttcc
     4621 tcaaccattg tttcaaaacc gtccacttcg tatgcactga caacagcgtt ttgcagaccg
     4681 gtgtgaacgc cgataacgta tttcactttt gaagcaacat ctttaccgat aacccagttg
     4741 cccaacgtac gcgatttgag gttggcatcg tcttggttgt cgaaaaggta gtctaattct
     4801 tcgtcagtat ctaaatcgaa agcattgtgg attttgatgg cgagaatcag cccgtcgggg
     4861 ttaatctcgt taagtgggag agaagagaat ccaaagcggc gatctagttc ttctacgcta
     4921 ataccacccg gaccatgccc ggcaatttta tttttgagtt ttttacccaa gacagattta
     4981 acaaaatgaa ttaaggcaga ttcggcggcg agtgcttcga cttcagtcag atgatagctg
     5041 atgatatagc gaccgagttt cttgcatttg gagatggctt tgagtttccg atcgataatc
     5101 tcgccggaga ctgggtcttg tgaacgacta gcaacccatt catgctcgaa gatacgatta
     5161 ccgcagcctt tgccgatgta gaggattttg tcttttttca ggtcggtcag acaatagaca
     5221 taaaaccgac gctcgccgtt attcaaaacg gaaagggttg aatctgagaa tttttttatt
     5281 tttgcaacca ttttaacttc tcctatacag tttactgccg gtgatatttt cagacgacct
     5341 gtcggcggga acggataggg tcgtctgaaa acgcatcttt attcaaatgg atgcttactt
     5401 cgccttcact gcgccgccga acgcctgaat aatcggctcc agcagcgcgt atttcgtttt
     5461 caaagcagcc ttgttgacca ccaggcggct ggaaatgtcg acgatgtgtt cgactgcttc
     5521 caagccgttt gccttcaagg tgttgcccgt cgaaaccaag tccacaatcg catcgctcaa
     5581 gccgaccagc ggcgcaagtt ccatcgagcc gtacagtttg ataatgtcca catggacacc
     5641 cttgccggca aaatgagatg ccgcgatttc aggatacttc gtggcaatct tcagacggca
     5701 tccgggttgc gaagctgctt cgtaatcaaa ccctttacgc acagcaacca tcatgcggca
     5761 cttggcaatc tccaaatcca aaggccggta aagccccgtg ccgccgtgtt cgatcagcac
     5821 gtctttgccc gcaatgccga agtccgccgc gccgtagcgg acataagtcg gcacatcggt
     5881 tgcgcggaca atgacaaggc ggatgttttc atggttcgtc ccgataatca gcttgcgcga
     5941 tttttcaggc tcttcagtcg gaacaatgcc ggcagcggca agcagcggca gcgtctcctc
     6001 aaaaatgcgc cccttggata aggcgatggt caaagcatta tcctgcatgg tgtggatgtc
     6061 ctttgtcggt tgaaacaata atgccgtctg aataggcgcc gacggtgcag acggcatacg
     6121 gttttatcag agcagagtcc ttacatcggc agcaatcgtt tccggctcgc ttccgtaagg
     6181 cgagaaaatg gcaacctcac cgtttttgtc gatgagatac gcaccggaag agtggtcgac
     6241 caaatagttt tcgctgtcgt ctttttgatt gactttggca gaaaccacgc ggtattgctg
     6301 cttgatgacc ggcaggtttt ggtcgcccgt tgccgtcaga ccgataaagt ccggattgaa
     6361 ctgtttggca tacttgccga tgatttcagg cgtgtcgcgt tccggatcga tgctgacgaa
     6421 caccactttc acgtccttag cctgcccgcc caactgcttc aacgtgtcgc tgtacgtcaa
     6481 aagctctgtc gggcagacat cgggacagtg cgtaaagccg aaagacagaa tcacgacctt
     6541 gcctttcaaa tcgctcaggc tgaaaggctt gccttcgccg tcggtcagcg tgaaatcgcc
     6601 gccgatgtct tccttacgca tatccgtacc gcgcgtttgc ggctttgccg cattttccgc
     6661 agctgacgcg gatgcacttg aagaagcggc ttgcgccgca ctgttgtctt gaggtttgca
     6721 ggcggcaagc gcggcaagta cgaaaacgcc cggcaaaaag gaacgcggta cggaaaacat
     6781 gacaatctcc caagaataaa tagcaaaacc tgattcgtac ggaatttgaa tatttccatc
     6841 ttaacgcgtt tggcagattt aatgtagtat ttttttacgt tgtttgcacg gcaggaattg
     6901 ccgaatcata cagaatcttt ggagcggaaa gaacagtaag attaaaataa taaaaattct
     6961 atttaaaatc aaaaataaaa taaaaacaga taaaataatt tcaaaaaaaa ggtttacaga
     7021 atttcaaaag ccattataat gacgacttct tcaccagccc aggtggcgaa attggtagac
     7081 gcaggggact caaaatcccc cgccgcaagg tgtgtcggtt cgagtccgac cctgggcacc
     7141 acaaccgctt ttagagcggt tatttttttg tctattgctg tttaatgaaa tagataaaac
     7201 taaatatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgcc gtactatttg
     7261 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata caatcaatat
     7321 attaagtcgt tgttagccct cttggttctg tcaatgccgt ccaatacagt ccggtgcaat
     7381 gagccatttt caggaagtat ggttgggaat atttttgata tatcaaaaat aaaatcaagt
     7441 tcaatcaata tcccgagtct ttattgggat gaaaccctgt aaattttaca attcttgata
     7501 tcgaagaagg gtttttcaca tctgcccctc ctacacaaac tgtcaacaat ttccatcttt
     7561 caaaaaaggt actcctgatt ttattctttt aaaatcaatc aataatttct ctgctttcaa
     7621 aaaatattaa aagatgaaag atttttcttg attgaactgt cggtttgcgt tatcgtttct
     7681 actctttttc acaaactctg tgttcctttc caattgattg gataatccgt ctgttgccgt
     7741 gttcccccaa tgcgcgtacc ctaaatcgct gcttggtgga attgcattac aggtgctgtg
     7801 gcaaagcggt ttgtccctat tggtttgaaa ccgtataaaa gaggtcgtta tgcagttatc
     7861 aggcgcgcaa atcatagtgc agagtctcaa agccgaaggt gtcgagtacg ttttcggtta
     7921 tcccggcggt gcggttatcg aaatctacga tgcccttttc caactcaata aattcaagca
     7981 cattctgacc cgtcacgagc aggcggcagt acacgcggca gatgcgtatg cgcgcgtcag
     8041 cggtaaggtg ggcgtggcat tggttacatc cggcccgggc gttaccaatg cactgaccgg
     8101 tattgctact gcctatacgg attcgattcc gatggtggtc atcagcgggc aggtaggcaa
     8161 ttccctgatt ggtacggatg cgttccaaga agttgatacg gtgggtatta cccgtccgtg
     8221 cgtcaaacac aatttcctgg ttacggacat caatgagttg gcggaaacca ttaaaaaggc
     8281 gttccaaatt gccgcaagcg gccgaccggg gcccgtggtg gttgatgtcc cgaaagatgt
     8341 tacgcaggcg atggcgaaat tcagctatcc tcaggaagac atttttatcc gttcgtatca
     8401 accggttgtt caagggcata tcggtcagat taaaaaggcc gtgcagatgt tggcatctgc
     8461 caaacgtccg gtcgtctatt ttggcggcgg cgtggtgttg ggtaatgctt ctgaagagct
     8521 gacccgattt gtccgaatga cgggtgctcc gtgcacgggt acgctgatgg gcttgggcgc
     8581 ttatccttcc ggcgaccgcc aattcctcgg tatgctcggt atgcacggta cttacgaggc
     8641 aaaccttgcc atgcagaatg cggatgtcgt tcttgccgta ggtgcgcgtt ttgacgaccg
     8701 tgtggtttcc gtaccgtcca aatttttcga gaaggcgaag aaggttatcc atatcgatgt
     8761 cgatccttcc agcatcgcca aacgcgtgaa ggcggacatt ccgattgtcg gcgacgtgaa
     8821 aaacgttttg tccgagatgg ttgcgctgtg gcaaaaacaa gagtccgtgc cgtctgaaga
     8881 tgctttgggc aaatggtgga aaaccataga ggaatggcgt tcccgcgatt gcttgtggtt
     8941 tgacaacggc agcgaaatta tcaagccaca atatgtgatt cagaagcttg ccgagattac
     9001 cggcaattcg gcaatcatca catcggatgt agggcagcat caaatgtttg cggctcaata
     9061 ttatcccttc gaacgtccgc gccaatggct caactccggc ggtttgggta cgatgggcgt
     9121 gggtctgcct tatgcgattg gtgcaaaact tgccgccccg gatcaagacg tattctgtat
     9181 caccggcgac ggctcgatcc agatgaacat ccaagagctg tccacctgtt tccaatatcg
     9241 gattccggtt aacgtcatta cactgaacaa cggttatctc ggtatggtac gccagtggca
     9301 ggaaatatat tacggcggac gagagtcgga aacctatttc gattctttgc ccgatttcgt
     9361 caaacttgcc gaggcatacg gccatatcgg tatccgcgtg gacaagaagt ctgatgtgga
     9421 aggtgccttg ttggaagcat tgaaccaaaa agacagtctg gtgtttatcg acttcctgac
     9481 cgaccagaaa cagaatgtga tgcctatggt tggcaacggc aaaggtttgg acgaaatggt
     9541 acttccgccg catatgcgtg cggacggaaa ggcgtaagga gagacaaatg cgacatatct
     9601 tatctgttct gatcgaaaac gaatcaggtg cgatgagccg cgtggtcggt ttgttctctg
     9661 cacgcgatta caatatcgat tctttggcgg ttgcgccgac cgaagacaaa accctgtccc
     9721 gtatgaccat cgttacacac ggcgacgagc aggttatcga acaaatcacc aagcaactca
     9781 ataaattgat tgaagtgatt aaagtggtcg atttgaatga aagccgtttt gtcgaacgcg
     9841 aactgatgtt ggtaaaagtc cgtgccgccg gcaaagaccg cgacgaattt ttacgcttga
     9901 ccgaaatcta ccggggcagc atcatcgacg taaccgaccg cagctatacg attgaaatta
     9961 ccggctcgac cgacaagctg gattcctttt tggaaacggt cggacgcgcc caaattttgg
    10021 aaaccgtacg tacaggcgcg gccggcatcg gtcgcggtga gcgtattttg aaaatttaac
    10081 gccgtaacct ttcagacggc acggtatttg aatgccgtct gaaaaacgaa cggcaggaga
    10141 gatttatgtc gaacattaaa attgtcgcac tggttaccgt caaaccggaa tacacagaaa
    10201 cactggtagc acagtttaaa gaactggtca aagccagccg tgcagaagag ggcaacatca
    10261 gctacgatct ccatcaggaa atcggcaaac cgaaccgttt tgttttcgtg gaaaattgga
    10321 aatcccaagc agctattgac gaacataacg ccagcgcgca tttccaagcc ttcgtccagt
    10381 ccgtcgacgg caaaaccgaa gcgttggaaa tcgtattgat gaatgaagtt gccgattaag
    10441 cggtacactc tgtttaaccc gtccgaagcc gctcaacatt ttaaggcttc gacaactatt
    10501 taccttacaa tcatttacct taaaggaaat caaatgcaag tctattacga taaagatgcc
    10561 gatctgtccc taatcaaagg caaaaccgtt gccatcatcg gttacggttc gcaaggtcat
    10621 gcccatgccg ccaacctgaa agattcgggt gtaaacgtgg tgattggtct gcgccaaggt
    10681 tcttcttgga aaaaagccga agcagccggt catgtcgtca aaaccgttgc tgaagcgacc
    10741 aaagaagccg atgtcgttat gctgctgctg cctgacgaaa ccatgcctgc cgtctatcac
    10801 gctgaagtta cagccaattt gaaagaaggc gcaacgctgg catttgcaca cggcttcaac
    10861 gtgcactaca accaaattgt tccgcgtacc gacttggacg tgattatggt tgctcccaaa
    10921 ggtccgggtc ataccgtacg cagtgaatac aaacgcggcg gcggcgtgcc ttctctgatt
    10981 gccgtttacc aagacaattc cggcaaagcc aaagacatcg ccctgtctta tgcggctgcc
    11041 aacggcggca ccaaaggcgg tgtgattgaa accactttcc gcgaagaaac cgaaaccgat
    11101 ctgttcggcg aacaagccgt attgtgcggc ggcgtggtcg agttgattaa agcaggtttt
    11161 gaaaccctga ccgaagccgg ttacgcgcct gaaatggctt acttcgaatg tctgcacgaa
    11221 atgaaactga ttgttgacct gattttcgaa ggcggtattg ccaatatgaa ctactccatt
    11281 tccaacaatg cggagtacgg cgaatacgtt accggccctg aagtggtcaa tgcttccagc
    11341 aaagaagcca tgcgcaatgc cctgaaacgc atccaaaccg gcgaatacgc aaaaatgttt
    11401 atccaagagg gtaatgtcaa ctatgcgtct atgactgccc gccgccgtct gaatgccgac
    11461 caccaagttg aaaaagtcgg cgcacaactg cgtgccatga tgccttggat tactgccaac
    11521 aaattggttg accaagacaa aaactgattg ttttcaaacg ggactgccta cacatcgtgt
    11581 aggcagt
//
LOCUS       DAWFAN010000055        11553 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWFAN010000055 DAWFAN010000000
VERSION     DAWFAN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.055"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..622)
                     /locus_tag="WGO65_001029"
     CDS             complement(<1..622)
                     /locus_tag="WGO65_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEZ0327332.1"
                     /translation="MSFSIALPLVYLFIGILIGKTPFRKIKGTFSVILSRWIIPFVIV
                     YNITTYRDGMQYVMLWMIISMGLIILIGKRVDKDPVWNLCLCYLNIGWLGLPIVATLF
                     GDEAAQVLISAYVGSSLFGNSVGVGLLLNNSNFISGLKKTLKSPPVVALVIGMFGIPL
                     GHFVADYFGEVYFFSKIAMGILGMVILGIWLAEIKLNLNDLKKSIKP"
     gene            complement(917..1222)
                     /gene="nuoK"
                     /locus_tag="WGO65_001030"
     CDS             complement(917..1222)
                     /gene="nuoK"
                     /locus_tag="WGO65_001030"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ0327333.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLNKLKG"
     gene            complement(1219..1890)
                     /locus_tag="WGO65_001031"
     CDS             complement(1219..1890)
                     /locus_tag="WGO65_001031"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ0327334.1"
                     /translation="MTFSAILFYILATIVLYGALRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTINPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(1902..2381)
                     /gene="nuoI"
                     /locus_tag="WGO65_001032"
     CDS             complement(1902..2381)
                     /gene="nuoI"
                     /locus_tag="WGO65_001032"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ0327335.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(2462..3538)
                     /gene="nuoH"
                     /locus_tag="WGO65_001033"
     CDS             complement(2462..3538)
                     /gene="nuoH"
                     /locus_tag="WGO65_001033"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ0327336.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            complement(3541..5802)
                     /locus_tag="WGO65_001034"
     CDS             complement(3541..5802)
                     /locus_tag="WGO65_001034"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ0327337.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVHSAIKCIAKDGNQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLASSKEELFMPLLSQEAVHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQIAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(6090..6527)
                     /locus_tag="WGO65_001035"
     CDS             complement(6090..6527)
                     /locus_tag="WGO65_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327338.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINKL"
     gene            complement(6527..7828)
                     /gene="nuoF"
                     /locus_tag="WGO65_001036"
     CDS             complement(6527..7828)
                     /gene="nuoF"
                     /locus_tag="WGO65_001036"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ0327339.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALAQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            complement(8218..8691)
                     /gene="nuoE"
                     /locus_tag="WGO65_001037"
     CDS             complement(8218..8691)
                     /gene="nuoE"
                     /locus_tag="WGO65_001037"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ0327340.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVAEYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(8691..9947)
                     /gene="nuoD"
                     /locus_tag="WGO65_001038"
     CDS             complement(8691..9947)
                     /gene="nuoD"
                     /locus_tag="WGO65_001038"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ0327341.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKKQPYEVYGKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     YHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(9937..10530)
                     /locus_tag="WGO65_001039"
     CDS             complement(9937..10530)
                     /locus_tag="WGO65_001039"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ0327342.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRHILTDYGFVGHPFRKDFPISGY
                     VEMRYDEAQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(10543..11025)
                     /locus_tag="WGO65_001040"
     CDS             complement(10543..11025)
                     /locus_tag="WGO65_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ0327343.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(11016..11372)
                     /locus_tag="WGO65_001041"
     CDS             complement(11016..11372)
                     /locus_tag="WGO65_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ0327344.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
BASE COUNT         2899 a         3015 c         2816 g         2823 t
ORIGIN      
        1 acggtttaat acttttcttc aaatcattca aatttagttt tatctcagcc agccaaatac
       61 cgagtatcac catccccaat attcccattg ctattttcga gaaaaaataa acttccccaa
      121 aataatctgc tacaaaatgc cctagcggaa taccgaacat tcctattacc aatgcaacaa
      181 cgggagggga ttttaaagtc tttttcaatc cggatatgaa attactatta ttcaataaca
      241 acccgacccc gaccgaattt ccgaataaag aacttcccac gtatgccgaa ataagcactt
      301 gcgccgcctc atctccaaac aaggttgcaa ctatgggaag ccccaaccat ccgatgttca
      361 aatagcacaa acacaaattc caaacaggat ctttatcaac tcttttcccg attaggataa
      421 tcaaccccat cgaaataatc atccataaca taacatactg cataccatca cgatatgtcg
      481 ttatgttata gacaataaca aaagggataa tccatctgct caatattaca gaaaaagtgc
      541 ctttaatttt tctaaaaggg gttttcccaa tcaaaatgcc aataaacaga tataccaatg
      601 gaagagcaat tgaaaatgac ataatcactc tccactttct gaaattctaa aattttcaaa
      661 aacccattgt tttacccaat tacccgccat acagcccaat ggctgccaat gagcccttga
      721 aattgtaaac attctctttc actcatacta atccacaatt tatgcatata tgggcgtgtg
      781 gagaccaaag tgccttgata tggcaaacaa gagtttttac ctgtaactgt ttgattaagc
      841 taactcctgc tgcacctaat ccaacagcag caatatcaaa cattatctat ctcctttttt
      901 ctttaaaaat tccaaatcaa cctttaagtt tgttcaaatc ggcaacgttg attgtttgtc
      961 ggttgcggta caccaatacc ataatcgcca aaccgatggc agattcggca gcggcaacgg
     1021 tcagtacgaa gaatacgaaa atctgtccgg cggtatcgcc caaatgttgc gagaaggcga
     1081 taaagttgaa gttcaccgcc aaaagcatca gctcgatgga catcagcaag accagcacgt
     1141 ttttgcggtt cataaagatg cccatcgcgc tgataccgaa caggagcgcg cccaatacca
     1201 gataatgcgt caaggtaatc atgctttgcc ctccccttcc ggcttgagac cgtctgaaac
     1261 ttcgctttct tcggcagatt cgacttgcgg tttgaccgct tccattttca ccagacgcat
     1321 acggccttgg tcggcgcgta ctttaacctg gtcggcagga tccatgcgtt tcggattgat
     1381 ggttttacgg tgaaccagcg caatcgccgc caccataccc aacaacagca ataccgccgc
     1441 caattcaaac ggcaacagat agtcggtata aatacggctg cccaaatcgc ggatattgtt
     1501 gtaatcggca ggaatgtctt tcatcagacc aaatgcggca aggtcggttt tcgggttgac
     1561 cagaatcagg atcagcgcaa ccgccaacaa tgtgccgacc acaccggcaa caggcgcgtg
     1621 ccgccagaaa ccggcacgca tttcttcaat gtcgatgttc aacatcatca cgacgaacag
     1681 gaacaacacc atcacggcgc cgacgtaaac caccaccagc gtcacgccca aaaactcagc
     1741 ctgcatcagc atccaaagca tcgcgctcac gcagaaggtc agcaccagat gcaaagcggc
     1801 gtggacaggg tttttagcgg taacggtacg cagcgcgccg tataagacga tggtggcaag
     1861 gatatagaac agaatcgcgg aaaaagtcat tcgtctgctc cttaacggta cggcgcgtca
     1921 gcggctttgc gtttggcgat ttcggcttcg tatttgtcgc cgattgccaa gaggatcggc
     1981 ttggtcatgt gcaagtcgcc ttttttctcg ccgtggtatt caaaaatatg cgtttccaca
     2041 atcgcatcgg ttgggcaggc ctcttcgcag aagccgcaga agatgcactt ggtcaggtcg
     2101 atgtcgtaac gcttggtgcg gcgggtgccg tcttcgcgtt cttccgattc gatattgatc
     2161 gccattgccg gacacaccgc ctcgcacaac ttacacgcaa tgcagcgttc ctcaccgttc
     2221 ggataccgcc gctgcgcgtg caggccgcgg aaacgcacgg attgcggcgt tttctcttcg
     2281 ggaaaataaa tcgtgtcttt gcgggcgaaa aagtttttga gcgttacgcc catacctttt
     2341 accaattcgc caagcagaaa ggtttttact aagttagcca tattatgttc cctcaaaaca
     2401 gggatttcgt taggtattca aaatcgcttt gttcagacgg cctcaagatg ccgtctgaaa
     2461 cttatttcca caaattcagc ggtgaaatca tccacacgcc caaaaccacg atgtaggcga
     2521 agccgatcgg aatcagcact ttccagccca aacgcatgat ttggtcgtaa cggtagcgcg
     2581 ggaaggttgc acggatccac aggtaccagt acagaaccgc cgccattttc acgaacatcc
     2641 agaatgcgga aggcgtaccg ataaagcccc agctttgcgg gaacggagac aaccagccac
     2701 cgaggaacat caacgatgtc agcgcggcaa tcagaatcat gaaaatgtat tcggcaagga
     2761 agaacagcgc gaacgcgaag ccggaatatt cgacgtggtg accggcaacg atttcagact
     2821 caccctctgc cacgtcaaac ggcgcacggt tggtttcggc aacggcggaa atcagataaa
     2881 cgatgaagat ggggaagagc ggcagccagt tccacgagaa taccgaaccg ccggcaatac
     2941 ctttggcttg cgcggcaacg atgtcggaga agttcatact gcccgatacc atcacgacgc
     3001 acaccagcgc ggcactcatg gcgatttcgt aagaaatgct ttgcgcggag gcacgcattg
     3061 cgcccaggaa cgaatatttg gagttggaag cccagcccgc gatgatcacg ccgtaaaccg
     3121 acagcgaggt aatcatcagg atgtacaaaa ggccgatatt gatattggtc agcacccatt
     3181 cttcgttgaa cggaatcact gcccatgccg caaaagacgg ggcgagcgac ataatcgggc
     3241 cgatgtagaa cagggctttg tttgacaact tcggacgggt tacttcttta aacaagagtt
     3301 tgaacacgtc ggcaaacggc tgaatcagac cccacgggcc ggttacgttc gggccgacac
     3361 gaagctgcat aaagccgatg actttacgtt cgaaatacgt caggtaggca acggtcagaa
     3421 tcagcggaat caggataatc acaattttga cgatgacgga taccaccaag cctacggtaa
     3481 tacccaaatc gcccagaccg agcgttgcgg caaagaggtt ttggaaccat tcttgcatga
     3541 tcaagctccc gccagttcaa tagtgtccat caacgcaccc agcgcggcat tttcggtatg
     3601 cagcggcaga tgcaccacgt tttcaggcaa accggcatcg gctttgacgg caaccgatac
     3661 gcttgcgccg ttttgtttgg cgacagcaat ttgtccgtct tgcaggccca agcgtgccaa
     3721 agtgttcgga tttacacgcg cggcaggcac ggcggcatgg ctggtttctt gcaacggtgc
     3781 ggaacggcgc acgatagaat cagtgtgata aataccgacg ccgccgacac ggacaaggcg
     3841 gtctgaggcc gtctgaacgc cctcccctgt ccatgtgcta cggttgtcca gtttggacgg
     3901 caggctttct gcatccagcg cgtctttcag aatcgcagcg gtatcgtgat attcaaaacc
     3961 tttcaggtca aacagattgc ccaatacgcg caatactttc cacatcggac gcgaatcgcc
     4021 gaagccttgt accacgccgt ggaaagattg cagacggcct tccatattga tgaagctgcc
     4081 tgaggtttcg gtaaacggtg caatcggcaa caacacgtcg cacacgtcca gcagcgtttc
     4141 gctgacaaac ggcgtaaacg ccatcacgct ttttgcctgt ttcaacgcgg ctacggcttt
     4201 tgcaccgtca accgtatcaa tttccggctc gacgttgagc aacaagactg cctgtttcgg
     4261 cgcgtttgcc atttcggcaa cgctctcgcc ggagtttaca ttcaagacat ccgcaccaac
     4321 actgttggcg gcttgcggca aaatacccag cactgcgccg gtcgcgtccg ccagctcttg
     4381 cgcggcggca taaatggcgg cgtaatcagg atggttttgc acttccgcgc ccaaaatcac
     4441 cgctgctttt tcagcgtttt tcaggctggc ggtaacggcg tgttccgcat cggcagacag
     4501 gtttttcaga cgacctgccc actcgtcggg atgtacggct tcttgagaca gaagcggcat
     4561 aaacaattct tctttactgc ttgccaatac gctcaaagcc atgcggtctt tagcggcgcg
     4621 gcgcaggcgg gcggtcagaa gcggctgttc tttgcgcagg ttcgcaccga ctaccaatac
     4681 ggcatcgttg tccgccagcg attcaatgct ttgtcccaac cattgcgcac ctttaatgcc
     4741 gtctgaaaga cgtttgtctt gttggcgcag acgggtggca aagtttttaa cacccaagcc
     4801 gtcggcaagc tttttggcca aatacagctc ttcaaccgta ttcatcggat tggcccaaac
     4861 gccgacttga ttttggttgc cgtctttggc gatacattta attgcgctgt ggacatattc
     4921 caacgcggtt ttccaatcca cgtccatcca ctcgccgccc tgtttgattt tcgggttttt
     4981 caaacggctt tcgtgataca ggccttcata ggcaaaacgg tcgcggtcag acagccaaca
     5041 ttcgttaatc gcttcgtttt ccagcggcaa cacgcggcgg acggtatggt ctttggtttg
     5101 tataatcagg ttgctgccca aagcgtcatg ggcggaaacg gatttgcggc ggttcaattc
     5161 ccaagtacgc gcgttgaagc ggaacggttt gctggtcaat gcgccgacag gacacaaatc
     5221 gatgacgttg cccgacagct cggtttccac cgttttgccg ataaagggca tgatttcgga
     5281 gtgttcgccg cgattcacca tcgcaatttc ctgcaaaccg gcgatttctt cagtgaaacg
     5341 aacgcagcgg gtgcagtgga tacagcggct catttcctcg gcggaaacca aaggccccat
     5401 gtctttgccg acgacggaac gtttttcttc ggtgtagcgg ctggtggttt ttccgtagcc
     5461 caccgccaaa tcctgcaact ggcattcccc gccttggtcg caggtcggac aatcaagcgg
     5521 atggttgatg agcaggaact ccatcacgcc ttcctgcgcc tctcgggctt ttgccgaatg
     5581 cgtacgcaca atcatgccgt ctgtaaccgg cgtggcacag gcaggcaggg gttttggggc
     5641 tttttctacg ttcaccagac acatacggca gttggcggcg atggaaagtt ttttgtggta
     5701 acagaaatgc ggaatataag taccgagctt gtgcgcggct tcaatcaccg tcgcgccctg
     5761 ctccacagat acttgtttgc cgtcgatttc gatttgtaac atggttcgtt cctagttacg
     5821 ggtatttgat aaataatctt atggaggtcg tctgaaaaat gggtttcaga cggccttttg
     5881 agtttcattg acaatcaata ctgtttaaaa cgtttactgc caccttgccg tcattcccgc
     5941 gtaggcggga atccattttt tgaatttcgg caactgcttt tcaaatatcg gtttctttag
     6001 attccactat ggattcccgc ctacgcggga atgacggcaa agttaaattt ttagcatttt
     6061 gcttttaacc aatataaaac caactaaaac tataacttat ttattatatt tttaaatgac
     6121 ttaaataact ctttaacagc acgctcattg tcttcatctg taggctcaaa cttttcacac
     6181 caattaatta tatttacatc gccatgcaat aactctgcat aacatcctac tgcatcgcaa
     6241 ctaccacccc gtgtccttga cgtgccaaaa ataaaatcac aattatgcga ataaagtttg
     6301 tataaatttc tcctaacctc gtattcatta tcaccagatg aataaaaacc aattctctga
     6361 ccttcatgat caaatactgc aacaaaatct aagccgtttc catgtctttc aaaaaagact
     6421 agaaatttag aaaatttccg acaaatctga ttaaaaagcg tattgagtgt cgtactctta
     6481 cctttgttgg ctgcaccgta tagaataaaa attttagctt ccaccattac caccctcccc
     6541 acttatgctc tttcatcggt ccgccgtgtt cgatgtaatg cacaaactca tcacggaaat
     6601 gcttggtaaa gctgcggaca gggaagacgg cggcatcggc gagggcgcag atggtgcggc
     6661 ctgccatttg gttgccgaca gaatccagca aatccaaatc ttccatttta cctttgcctt
     6721 ccacgatacg gtggacgatg cggtagagcc agcccgtacc ttctcggcaa ggcgtacatt
     6781 ggccgcaaga ctcgtcgtag tagaagtagc tcaggcgctc aagggctttc accatgcaca
     6841 cgtcttcgtc catgacgata atcgcgccag aacccagcat cgagcctgct ttggaaatcg
     6901 agtcgtagtc catatttgtc tgcatcatga tgtcggcagg caatacgggc gcggacgaac
     6961 cgccaggaat aacggccttg agttttttgc cgccgcgcat accgcctgcc attttcaaaa
     7021 cttcggcaaa cggcgtgccc aacggcactt catagttgcc cggacgctcg acatgaccgg
     7081 aaatacagaa taatttggta ccgcctgcat tcggaatacc tttatcggca aatgcctgtc
     7141 cgccatcacg gataatgaag gggacggaag agaacgtttc ggtattgttg atggtggtcg
     7201 gcttgccgta caggccgaac gaagcaggga acggcggttt gaagcgcggc tggccttttt
     7261 tgccttccaa cgattcgagc aatgcagttt cttcgccgca aatatatgcg ccgtagccgt
     7321 ggtgggcgaa aagttcgaat tcaaaatccg aacccaaaat atttttaccc aaaaaacctg
     7381 cggcacgcgc ctgcgccaaa gcggcttcaa aacgttggta gccttcgaag atttcgccgt
     7441 ggatgtagtt gtagccggct ttcgcaccca tcgcgtaacc ggcaataatc atgccttcaa
     7501 tcaaggcatg ggggttgaac ataatgatgt cgcggtcttt aaatgtaccc ggctcgcctt
     7561 cgtcggtatt gcaaaccaca tatttttcgc ccgggaagga acggggcata aagctccatt
     7621 tcagaccggt cgggaagccc gcaccgccgc gaccgcgcaa accggaggtt ttgacttcgt
     7681 caatcacatc ggtttgcgag atgttttcgg acaggatttt acgcagggcg gtataaccgc
     7741 cacgtttgac gtattcgtcc aatgtccagc aatcgggatt ggcggtatcc acttggtcaa
     7801 aaatcacgcc tgattggtaa atagccattt ttggtgtgcc tgtttgtttt cgtatcggtt
     7861 gcggccgctg tttcagacga ccttaagatg tctttgtgta ccggcttgta acgtcgtctg
     7921 aaataaaatc tagtttatca aatcgatatt ttcagacgac ctttaatcgt ttttgttgga
     7981 tctcgaccac ttgcttgtct gtcgtcattc ccgcgcaggc gggaatccat cctcaatggt
     8041 aagcaatgtc ttattaaatt cagaaaccga atcttaccgg tggattcccg cctgcgcggg
     8101 aatgacggca tttcggtatt tcagtagggc ggattcttaa atccgacatt ttgccttttt
     8161 acccactctg tcggctacaa gtatccgacc tacgtttaaa tcgtcgtttc agacgaccta
     8221 ctccaactcc gccagtttct tctcaatcgc ttcttcggtc ataaagctgc acatgctgtg
     8281 gttgttgacc agcataacgg gagcgtcgcc gcacgcgccc atgcattcgc cttcgacaag
     8341 agtaaatttg ccgtcgggcg tggtttcgcc gtagccgata ccgagtcttt gtttgaggta
     8401 ttcgccggta gccataccgc cgcgcagggc gcagggcagg ttggtacaaa cggtcaattt
     8461 gtatttgccg acaggctcaa ggtcgtacat attgtagaag gtagcgactt cgtaggcttg
     8521 tgcaggcgag atgccgatgt attcggcaac aaaagcgatg gtttcgggag cgagccagcc
     8581 tttttcggtc tgggcaatac gcaatgcacc cataatggcg gagcggcgtt ggtcggcagg
     8641 gtatttcgcc aactctatgt cgatttgttt taaggattct gcggataaca ttatcggtca
     8701 acctccccga atacgatgtc ctgcgtaccg atgatggcaa cgacatcggc gagcatgtgg
     8761 ccttttgcca tttcgtccat gccttgcagg tgggcgaagc cgggtgcgcg gattttcagg
     8821 cggtagggtt tgtttgcgcc gtctgaaatg atgtaaacgc cgaactcgcc tttcgggtgt
     8881 tcgacagcgg tgtaggtctc gccctcggga acgtgcatcc cctcggtaaa gagtttgaaa
     8941 tggtaaatca ggtcttccat acctgttttc atttcggtac gtttgggcgg agcaaatttg
     9001 tggtttgtgg taatgaccgg acccggatta acacgcaacc actcggaaca ttgtttgatg
     9061 atgcgtacgg attgacgcat ttcttccata cggcagaggt agcggtcgta gcagtcgccg
     9121 ttgacaccga cggggatgtc gaaatccatt ttgccgtaca cttcgtaagg ctgtttctta
     9181 cgcacgtccc attccacgcc cgaaccgcgc aacatcacgc cggtaaagcc tttttgcatg
     9241 gcgcgttcgg gggagacgac gccgatgccg acggtacgct gtttccaaat acggttgtcg
     9301 gtcaggaggg tttcgagtgt gtcgatattt ttcgggaaac gttcgcagaa ggcatcgata
     9361 aagtcgagca tcgtgccttc gcgggattcg ttgagctgct tcaatacttt ggcattgcgg
     9421 aatttgctgc cctcgtattt aggcatgaag tcgggcaggt cgcggtaaac gccgccggga
     9481 cggaagtagg cggcgtgcat acgcgcgccg gaaacggctt cgtacaagtc catcagctct
     9541 tcacggtcgc ggaaggcgta aagaatggcg gtcatcgcgc cgatgtcgaa ggcatgcgaa
     9601 ccgatgccca tcaagtgatt gaggatgcgc gttacttcgg caaacatcac gcggatgtat
     9661 tgggcgcgga taggcacatc gataccggca agtttttcta ccgccaagca atacgcctgc
     9721 tcgttgacca tcatggaaac gtagtccagc ctgtccatat aaggcagggc ttgcaggtag
     9781 gttttggttt ccgccagttt ctctgtgcct cggtgcagga ggccgatatg cgggtcggca
     9841 cggacgattt gttcgccgtc cagctccaaa atcatacgca atacgccgtg cgccgcaggg
     9901 tgttgcgggc cgaagttgat ggtgtagttt cttaatttat tggccaccgt agttctcctc
     9961 acggacgata cgcggcgtga tctcgcgcgg ctcaatggta acaggttggt aaatcacgcg
    10021 tttttgcgct tcgtcgtaac gcatttccac atagccggaa atcgggaagt ctttgcggaa
    10081 cggatgtccg acgaagccgt aatcggtcag gatgtggcgc aggtcgggat ggttgttgaa
    10141 catgatgccg tacatatcaa aggcttcgcg ttcgtaccaa tccgcgctgt tgtaaatatc
    10201 gactacggat tcgactacgg ggaagtcgtc gtctgaaacc cagacgcgta cgcggatgcg
    10261 ttgattgttt ttaacggaaa gcaactgact gacgacggca aagcgtttgc cctgccatac
    10321 ttcgtttttg taagtgctgt aatcgacacc gcacaagtcg accagaagct cgaaatgcaa
    10381 ctcttcatgg tcgcgcaatg cggtcatgac tgaaatatag tgttcgggca gacactcgac
    10441 ggtaatctca cccaaagcgg aaatgacttt gcctgcctga ttgcccaaaa cgcggctgac
    10501 ggtttcgtat aagttttgaa tgcttgccat atcgtcctct ccttactcgt cacgcgcgat
    10561 ggtggaagtg cgcttgattt tttgttggag ctgaatcaga ccgtaaatca gggcttccgc
    10621 agtcggcgga caacccggca cataaacgtc taccggcacg acgcggtcgg caccgcgcac
    10681 aacggaataa gaatagtgat aataaccgcc gccgttggca cacgagccca tagacaatac
    10741 ccagcgtggc tcggcgagct ggtcgtatac gcggcgcagg gcgggcgcca ttttgttggt
    10801 cagcgtaccc gccacaatca tcaggtcggc ctgacgtggg gacggacgga aaatgatacc
    10861 gaaacggtca aggtcataac gcgccatacc cgcatgcatc atttccacgg cgcagcaggc
    10921 caagccgaaa gtaaccggcc acaacgaacc ggtacgcata tagttcaaca ccgtatccgc
    10981 gctggtggtg atgaaacctt ttttcaaaac gccttctatt cccattccag cgcacctttt
    11041 ttccattcgt aaacaaagcc taccgtcaga acgacgataa acaccaacat agaccagaag
    11101 ccgtacgcgc ccaaatcttt gaacacaact gcccacggca acatgaacgc gacctccaaa
    11161 tcaaacagga tgaagaggat ggcgacgagg taatagcgca cgtcaaactt catcctcgca
    11221 ttttcaaagg cttcgaaacc gcattcgtaa ggcgcgtctt tttcggcata gtggcgtttc
    11281 gggcctaaaa tcgtgccgag caggataaac agtacgccgg ccgcgaggcc gacgaggata
    11341 aagacaaaga cgggaaagta agcggacaac atggttacac ccaaatccgt taacaaaatt
    11401 tctacaataa tttcgtattt tagcgaattt caaaaaccat taaaaggtaa atatcggcaa
    11461 aacgcccaaa aaaacccaat aaatacaatc atgttatgat aacgattctt atttgatttt
    11521 ataaggtcat ataaattttt acaccgtaat tcg
//
LOCUS       DAWFAN010000056        11516 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWFAN010000056 DAWFAN010000000
VERSION     DAWFAN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.056"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            122..544
                     /locus_tag="WGO65_001042"
     CDS             122..544
                     /locus_tag="WGO65_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327345.1"
                     /translation="MKKTFLSAVLLLSAATQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGWGRGRWATMKFQNGEFMYTVDAGFDSV
                     THAVGSGVLVERRGKEIARFDCTSKTAQANFDDDDFAW"
     gene            562..1323
                     /locus_tag="WGO65_001043"
     CDS             562..1323
                     /locus_tag="WGO65_001043"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ0327346.1"
                     /translation="METVKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEMAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            1338..2672
                     /locus_tag="WGO65_001044"
     CDS             1338..2672
                     /locus_tag="WGO65_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0327347.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLEIAVSGLPLILDGVLKPSVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAETAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTTAENQEAEQATSEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            2669..3886
                     /locus_tag="WGO65_001045"
     CDS             2669..3886
                     /locus_tag="WGO65_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ0327348.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            3961..5025
                     /locus_tag="WGO65_001046"
     CDS             3961..5025
                     /locus_tag="WGO65_001046"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ0327349.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     MQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            5211..6590
                     /gene="radA"
                     /locus_tag="WGO65_001047"
     CDS             5211..6590
                     /gene="radA"
                     /locus_tag="WGO65_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ0327350.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTAAEVPRNPTGMSELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(6866..7345)
                     /locus_tag="WGO65_001048"
     CDS             complement(6866..7345)
                     /locus_tag="WGO65_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ0327351.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTIGE"
     gene            complement(7477..7836)
                     /locus_tag="WGO65_001049"
     CDS             complement(7477..7836)
                     /locus_tag="WGO65_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ0327352.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(7875..11489)
                     /gene="recB"
                     /locus_tag="WGO65_001050"
     CDS             complement(7875..11489)
                     /gene="recB"
                     /locus_tag="WGO65_001050"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ0327353.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSNDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRAYACEKDGITMLKRNWQRVTDNAPADTDFAFTEDAPPPAAYRGNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEMWLPAVAEM
                     AEACRKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLGADASAYTQQAMDEAVTHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGTDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         2487 a         3045 c         3429 g         2555 t
ORIGIN      
        1 gtgccgccct atgtgttgcc ctaatttgga aggggttaca cccttttcaa ataaaatctg
       61 atgctgctgc cacgaaggac ggatgtccaa gtggcggggt tttaaccatt aaggaaatac
      121 gatgaaaaaa acgttccttt ctgccgtatt gcttctgtca gctgcgaccc aaaccgtgtg
      181 ggcagatacg gtgttttcct gtaaaacgga caacaacaaa tatatagaag ttcaaaaaat
      241 taaccgcaat ctttacgaat attcgttcgg cagtgcggca aaaaaagaaa ttgccatacg
      301 caacagcaaa gctgatctgt tggggcgttc cgacaggtgg caaggctggg gcagaggccg
      361 ttgggcgacg atgaaattcc aaaacggcga atttatgtac accgtagatg caggcttcga
      421 ttccgtgacc catgcagtag gcagcggtgt ccttgtggag cgtaggggca aggaaatcgc
      481 acggttcgac tgtacgtcga aaaccgcgca ggcgaatttc gacgatgacg attttgcctg
      541 gtaatcgggg cggataaggc gatggaaaca gtgaaaccca tcatgctgat tgtccgaccg
      601 tcgggcaggg cgaaggacga tgtggaaacc tgccgtcgtg ccggatggca ggcggaagtg
      661 ttgagcccga ttgaaatcga agcagatgct gccggactgg aacttttgtc cgaacaatat
      721 gcccgtgcgg atgccgtgtt ttgggtcagt ccgaccgccg ttgaaaccgc cgtcccgtac
      781 cttaaccttt cagacggcat aaaggcgcag attgccgtag ggcagggcag ccgccgcgca
      841 ttggaacgct gtttggtcag aacggtcatc gcgcctgatg acggcaacga cagcgaggcg
      901 gttttgcgcc tgccggtttg gaacagtctg cccgaaggtg cgcgcgtatt gtttgtgcgc
      961 ggacacggcg ggcgggattt tctgatgaat gccttgcagg agaaaggttt tcggacggag
     1021 atggcagaag tctatttcag acggcataaa cctttgaact ttcaaaattt ccaaaccgaa
     1081 aatattgccg ccgcctatat tacgtcgacc gagctggtgc gcttgctgtt cgggcagctt
     1141 ccgccgcaat tttcccgatt cttcaaatcc ttgctatact ttacccatca tccgcgcatt
     1201 gcggaggcat tgaagcgcga aggcgtgtgt tcggtcgaaa ccgtccctac gctggaagcc
     1261 gcgctttccc attcttccat ttccgtttca gacggcatgg tctttcccgg aacctcaaat
     1321 taataaggag caaaacggtg ggcgaacctg aaaacaaatc atccgaaccc gtacgcgaga
     1381 tacaggcatc aaaagaaatg ccgtctgaaa cctcttcccc acgcaaagaa aacgaaacag
     1441 aagtacacat tcctgccgct ccttttatcg tcaaacagtc cggcagcaac gctttggcag
     1501 tctgcgccct ggtattggcg gcattgggtt tgggtacaag tggttttttg tttgtccaag
     1561 gacagaatgt cttgaaaaac caagagctgg cattcaacca aaaaatcgac aaagccgctt
     1621 tgggcgagtc ggaaaacgcc gccctgctca aagacaacct caaccggcaa gccgccatac
     1681 aatcagagct cgaccgtttg gacggaaacg tcaaagcaaa cggcgaacaa atcttggaaa
     1741 tgcaaaaatc ctatcgcgag ttgaccaaag gacgcgccga ttggctggtg gacgaaaccg
     1801 agaccatact caatctggcg gcgcaacagc tggtgttgac tggcaatatc caaacggcag
     1861 tcggcgtatt ggagcatatc gacagccgcc tgtcccgttt caatcaggca gagcttctgc
     1921 cgatcaagca ggcggtcagc agcgacttgg cggaactgaa aaaccgtccc tatgtcgata
     1981 tttccggcac ggcattgcgc ctcgacaggc tggaaatcgc cgtatccgga ctgccgctga
     2041 ttttggacgg cgtattgaaa ccgagcgtac aggcacgcaa cgaagccgtt tccgcttcat
     2101 ggtggcagaa cgtatgggaa aaatccctcg gcacattgaa ggggctggtc gaaatccgcc
     2161 gtttggaaaa caacgatgcc atgctgattt ctcccgaaca ggcctatttt gtgcgtgaaa
     2221 acctgcgtct ccgccttttg gatgcgcgga ctgcattaat gcagcgcaac agcgaagtct
     2281 atcagggcga tttgaacaat gccgaaaccg ccgtcagaca gtattttgat gccaaatccc
     2341 ccgccacgca gtcgtggttg aaggaattgg cggaattgaa ggcgttggat gtgcgcatga
     2401 cggcggatga cggtttgaaa aacagcctaa atgccgtccg cgcctatcgc gacggtacgc
     2461 gcatgacgac ggcggaaaat caagaagcgg aacaggcgac ttccgaaccg gcaaacgaaa
     2521 acacagcttc cgaaccggct gccgcatcgg atgtgaagac catagaagca ccgtccctgc
     2581 cttcggaacg caaaccggaa cagcctgcaa aaaaacagcc cgtaccggaa aaggcagggc
     2641 gttcgccgtc cgctaaagga gaacgcgcat gaaaacggta gtctggattg tcgtcctgtt
     2701 tgccgccgcc gtcggactgg cactggcttc gggcatttac accggcgacg tgtatatcgt
     2761 actcggacag accatgctca gaatcaacct gcacgccttt gtgttaggtt cgctgattgc
     2821 cgtcgtggtg tggtatttcc tgttcaaatt cattatcggc gtattcaata tccccgaaaa
     2881 gatgcagcgt ttcggttcgg cgcgtaaagg ccgcaaggcc gcgcttgcct tgaacaaggc
     2941 gggtttggcg tattttgaag ggcgttttga aaaggcggaa ctagaagcct cacgcgtgtt
     3001 ggtcaacaaa gaggccggag acaaccggac tttggcattg atgttgggcg cacatgccgc
     3061 cgggcagatg gaaaacatcg agctgcgcga ccgttatctt gcggaaatcg ccaaactgcc
     3121 ggaaaagcag cagctttccc gttatctttt gttggcggaa tcggcgttga accggcgcga
     3181 ttacgaagcg gcggaagcca atcttcatgc ggcggcgaag atgaatgcca accttacgcg
     3241 cctcgtgcgt ctgcaacttc gttacgcttt cgacaggggc gacgcgttgc aggttctggc
     3301 aaaaaccgaa aaactttcca aggcgggcgc gttgggcaaa tcggaaatgg aacggtatca
     3361 aaattgggca taccgccgcc agctggcgga tgctgccgat gccgccgctt tgaaaacctg
     3421 cctgaagcgg attcccgaca gcctcaaaaa cggggaattg agcgtatcgg ttgcggaaaa
     3481 gtacgaacgt ttgggactgt atgccgatgc ggtcaaatgg gtcaaacagc attatccgca
     3541 caaccgccgc cccgagcttt tggaagcctt tgtcgaaagc gtgcgctttt tgggcgaacg
     3601 cgaacagcag aaagccatcg attttgccga tgcttggctg aaagaacagc ccgataacgc
     3661 gcttctgctg atgtatctcg gtcggctcgc ctacggccgc aaactttggg gcaaggcaaa
     3721 aggctacctt gaagcgagca ttgcattaaa gccgagtatt tccgcgcgtt tggttctggc
     3781 aaaggttttt gacgaaaccg gagaaccgca gaaggcggag gcgcagcgca acttggtttt
     3841 ggcaagcgtt gccgaggaaa accgaccttc cgccgaaacc cattgaaacc ttcagacggc
     3901 atcgttgaaa tgccgtctga aaaccatttc acttgacaaa cagactgaca gaaaacccgt
     3961 atgactcttt taaaaaacga taccttcctc cgcgccctgc tcaaacagcc cgtcgaatac
     4021 acgccgattt ggatgatgcg ccaggcgggg cgttatctgc ccgaatacaa agccacacgc
     4081 gcgaaagcgg gcagcttcct cgatttgtgc aaaaacaccg aattggcgac cgaagttacc
     4141 atccaacctt tggaacgctt cgatttggac gcggcgattt tgttttccga catcctgacc
     4201 gtccccgacg caatgggctt gggactgtat tttgccgaag gcgaaggccc gaaattcaaa
     4261 cgcgccatgc aacacgaggc cgacatcgcc aagctgcacg ttcccgatat ggaaaaactg
     4321 caatacgttt tcgacgcggt aacttccatc cgtaaagcat tggacggccg cgtaccgctt
     4381 atcggctttt ccggcagccc gttcacactc gcctgttata tggtcgaagg cggcggcagc
     4441 aaagaattcc gcaccatcaa aaccatgatg tactcgcgcc ccgatttgct gcacaaaatc
     4501 ctcgatacca acgcccaagc cgttaccgcc tacctcaacg cccaaatcga cgcgggcgcg
     4561 caggcggtgc aaattttcga cacttggggc ggcgtgttga gcgacgcggc gtttaaagaa
     4621 ttcagcctca aatacatccg ccagatcgtc gccggactca aacgcgaaag cgaaggccgc
     4681 cgcgtgcctg ttatcgtatt tgccaaaggc ggcgggctgt ggctggaaag tatggcccaa
     4741 atcggcgcag acgcattggg cttggactgg acgtgtaaca tcggcgaagc acgccgccgc
     4801 gtcggcaagc aagtcgccct gcaaggcaac ttcgacccgt tcgccctctt cggtacgccg
     4861 gaatccatcc gcaccgaggt cgcacgtatc ctagccgact acggacacgg cagcggccat
     4921 gtcttcaacc tcggacacgg catcaaccaa cacgccgacc ccgaacacgc caaaatctta
     4981 gtcgataccg tacacgagct gtctcggcag tatcacggcg ggtaagccgg caggaaaccg
     5041 cccgatatgc cgtctgaagc cgagagatgg ccggttaggg taaaaataag gcaatgcggc
     5101 aatatccgcc gtgtacggat agtacatgac ggcggcgttg tcgtattggc gcaatcccaa
     5161 ccgtccctat gttcagacgg catttttgtt ttcagatgca gggaaaaccg atggcaaaaa
     5221 cgcttaaaac cctttaccaa tgcaccgaat gcggcggcac ttcgccgaaa tggcaaggca
     5281 aatgcccgca ttgcggcgag tggaacacgc ttcaagaaag cttagccgcg cccgagccga
     5341 aaaacgcccg cttccaatct tgggcggcgg atacctcgac cgtccaatcc ctctccgccg
     5401 ttaccgctgc cgaagtgccg cgcaacccga ccggcatgag cgaactcgac cgcgtattgg
     5461 gcggcggttt ggtcgatggc gcggtcatcc tactcggcgg cgatcccggc atcggcaaat
     5521 ccacgctgtt gttgcaaacc atcgccaaaa tggcgcaaag ccgcaaagtg ctgtacgttt
     5581 ccggcgagga atccgcccaa caagtcgccc tgcgcgcgca gcgtttggaa ctgcccaccg
     5641 aaggcgtgaa cctgcttgcc gaaatccgca tggaagccat tcagacggcc ttgaaacagc
     5701 atcagcccga agtcgtggtt atcgattcca tccaaaccat gtattccgac caaatcactt
     5761 ccgcccccgg ctccgtgtcg caggtgcgcg agtgtgcagc ccaactgacg cgcatggcga
     5821 aacagatggg catcgccatg atactggtcg gacacgtgac caaagacggc gcgattgccg
     5881 gcccgcgcgt gctggaacac atggttgata ccgtgctgta tttcgagggc gaccaacatt
     5941 ccaactaccg catgatacgc gccatcaaaa accgcttcgg cgcggcaaac gaactgggcg
     6001 tgttcgcgat gacggaaaac ggtttgaaag gtgtgtccaa cccgtccgcc atcttcctcg
     6061 ccagctaccg cgacgatacg cccggctcgt gcgttttggt tacacaggaa ggcagccgcc
     6121 cgcttttggt cgaaattcag gcattggtcg atgacgcgca cggcttcacg cccaaacgcc
     6181 tcaccgtcgg actggaacaa aaccgtcttg cgatgctgct tgccgtgtta aaccgacacg
     6241 gcggcatcgc ctgtttcgat caggatgtgt tcctcaacgc cgtcggcggc gtgaaaatcg
     6301 gcgaaccggc ggcggatttg gcggtcatcc tcgcgatgct ttccagcttc cgcaaccgcc
     6361 ctatgcctga aaaaaccgtg gttttcggcg aaatcggctt aagcggcgaa gtccgccccg
     6421 tcgcacgcgg gcaagagcgg ctcaaagaag cggaaaaact cggcttcaaa cgcgccatcg
     6481 ttcccaaagc caatatgccg cgcaacgcca aagagtttcc gaacctgaaa atctacggcg
     6541 tttcgagttt gcaggaagcc atcgatattt gccgtgacag cagggaataa acggaaatgc
     6601 cgtctgaaat caggtttcag acggcatttg gtttgtggcg gattgaaaca agaaggcata
     6661 ccggcgacag ataagatttg tggcaaagtt gcctgtgatg tggcaaaaac acacacgccc
     6721 gtcatccccg cgagggcggg aatccggaat cgtccgtttc ggcaatgatt gaaaatcacg
     6781 gcaatccaac cgcttggatt cccgacttcg cgggaatgag gggcgtgtgc gtttgatttc
     6841 catccgccat atgtcggcaa cgggcttatt cgcctatggt tttttgtatc agtttttcgg
     6901 cgtttgccaa agtgtttgcc acttcgtcga aaccgatgcg gctgccggcg atgagggcgc
     6961 gcgtatcggt ataggcggcg cgtactttgc cgtccgtttc ggtaacgagg acgcgcaggg
     7021 gcagttgcag ggcgaaggcg gggtctttga ccatcagcgg cgtaccggct ttgggcgtgc
     7081 cgaagacgat gacttttgcc ggctgcatcg ttaagccgtt tcggcgggcg gcttcctgat
     7141 ggtcgatgac ggcaaaaatg tccatccctt tgctttttat ggcggtttca aggcggctga
     7201 cggtttcgtc aaaactgtat tttgaggtga gggtatgcgt ggtcatagcg gtttcgtttt
     7261 gggtggacgg ttcgctggca ggatgtgccg aagcggttga aatgcagagt gcggatgcgg
     7321 caatcagggg gagtatgtgt ttcatcgtat ttcctttttc cttttcggtt tgtttatcag
     7381 gcttcggacg gggcggcacg cgccgcgccc gatgttgcgc cgcgtgccgg aacgccgtat
     7441 gccgtctgaa agcctgtcct ttcagacggc attgcgtcat ttcatccctt ttttgagcag
     7501 gtcttcataa ccgccgtgat tggcaacatt tgtataacct gcttttttca gctcttgaag
     7561 ggcggcttcg gcacgccgtc cgctgcggca gtagaggttg acgggcgtgt ctttgtcggg
     7621 cgcggcttcg tgtatgcggc ggacgatttg gtcgacgggg atgttgaccg cgttgtgcaa
     7681 atgcccttcg ctaaattcct gttcggaacg gacatcgatc caaacggccg gatgttgcgc
     7741 ggtttgggcg gcggatacgg gtttttgcgg ggctgcctgc gcggcaaagg ctgctgaggc
     7801 aatgagtgcg gcggtaatca ggtgtttgat attcataggg ttctcctgcg gttgtcgttt
     7861 taagggacgg gaagttattt tatctgttcc aaagcggcgg catctatgtc ccaacgccaa
     7921 acgccgccgc ctttgccgtc tgtgccgcgc aaaaacaggt agcggacgga aacggcggcg
     7981 ggcggttgtc cgcgcagttt gaagtagcgt gcggcggcaa cggcgtaaat cagcgcctga
     8041 aggtaatagt ggtggtgcgt gacggcttcg tccatcgcct gttgcgtgta ggcggatgcg
     8101 tccgcgccga gatggtttga tttgtagtcg atgatgcaga tattgccgtc ggggtcttgg
     8161 cagaccatat cgataaagcc gtttaaaaag ccgttgacgg tgtggaagtc gagcgtttcg
     8221 gcggcggcac ggcagacttc gggcagcctg atgtcgtcgc gggcaaacca gtcgcgcagc
     8281 cgtttgaggc cgaagtcttc ggtatggagg gtaaagccca tttcgggaca gcggcactcg
     8341 ggcggaatgt cggacaggca gtatgccccc gtcagcggcg ttttacggca ggcttccgcc
     8401 atttcggcaa cggcgggcag ccacatttct tcaaacccgt atttcttcag cttgtcggca
     8461 atgagggttt cctgtccggc ggctgcttgt ccgaatttga aatcttcaag aatttcgtgc
     8521 aggcacagcc ccgcctgcgt gcctttcgga aaatcgtgta tcgatatgcc gtctgaagcc
     8581 gtcggcgttt cagacggcat cgccggcacc gaggtttcgg cggcatccaa ggacgggcag
     8641 gcatcttctt cgccgccgtc gggcgtttgg gtatggcggc ttaaagcggt aaagctggtg
     8701 tggcggacaa atcggaatct gcgttcggga atgctgtttg cggcaaattc ggcggtttga
     8761 ccggcgttgc cgcgataggc ggcagggggc ggcgcatctt ctgtgaaggc gaaatccgtg
     8821 tcggccgggg cgttgtccgt cacgcgctgc cagttgcgtt tgagcatcgt tatgccgtct
     8881 ttttcacacg cataggcacg gcggacggtt tcacggctgt cttgcggcaa gccttcaatc
     8941 aggtaggcga gggggttgtc ggcggtatcg gaagaatagg cggcgtagat gttgagctgt
     9001 tcctcggcac gcgtcagcgc gacataaagc aggcgcaggc gttccgccat ttcttcgtcg
     9061 gcgtattgct tctgttcgtc ttccgacagt tgcgcctttg ccaacagttc ggttcggttg
     9121 gcgctttggt ggaggatttg ccagtcggac ggcccggtat cttgcgcgtc ccacgcaaac
     9181 gggcagtaca ccagcggata ctgcaaacct ttcgaggcgt gcatggtaac gattttgacc
     9241 aaatcttcgt cgctttccag acggatggcg cggttgtcgt tgctgttgtt ttcggcaagg
     9301 ctgatttggt cgcccagcca cttgtgcaaa gcggcggggt tgcggttttg ggcgtcttcg
     9361 gcggcaagca gttcgagcag ttggaaataa ttggtcaggc tgcgcccgtt gttccggctt
     9421 aagaggcgcg tttcgatgcc gtgtgtttgg gaaaattgct gcatggcggc gaaaatgccg
     9481 tattgattcc agttgtcgag cgcggttcgg gcagattccg cccaatgcaa aatctcgctt
     9541 tcgttttggt tgaagtcgtg caattgctgc gcgtcataac cgaatatgcc gcttgtcagg
     9601 acaaagcgca gcgttccggc gcggcgcggt tcgagccaga agccgatgag tgcggaaagg
     9661 gcggcggctt cgggcgaggc gaacacggat tcgcgcgaaa gcaggacgct ttgcacctgc
     9721 cgttttttca gggcggcgga aaccatcacc gcctcgttgt gcgtgcgtac cagcacggca
     9781 atatcgcccg actgcaacgg gcagcctttg aaattcagac ggcctccggc ggcttcgttg
     9841 agcgcgtggg cgatttcgtc ggcgcaatag tcggcggcac gtcggcgcaa aacgtctttg
     9901 ttggcttttt cattgtcgtt ttcgtgcagc caacgaacct gtacggcagg acgtttgggc
     9961 gacaatctgc tttcggcacg cgccgcaccg acttccgaat agccgatgtt ttccaaaacg
    10021 aacgggcgtt ctttgaggcg gaacagcgcg cctatgctgc cgataagcgc ggcgtggctg
    10081 cggtagttgg tggcgagcgt gtagcggtgc cgcgcgtctt ccgccgcctg aaggtaggcg
    10141 taaatgtccg ctccgcgaaa gctgtaaatc gcctgtttgg gatcgccgac gaggaacagc
    10201 ggtcggtttt gggcgatgaa gattttttgg aagatttcgt attgcagcgg gtcggtgtct
    10261 tggaactcgt cgatcagcgc ggtttcccag ttttcggcaa cggcgcgggc gagggtttcg
    10321 gcgtgcggat tgtcggtcag cgcggtgtgg acatcgagca gcaggtcgtc gaaaccgcgt
    10381 tcgcggcgcg attttttcat ctcggcaagg ctgcggttga ggtattcgat taaatccagt
    10441 tgcagccgga tcattgttgc ttcttccgct tcttcgagtg cgttcaaatc gcgcccgaag
    10501 tctgccagtt tctgcaattc ggcaaatact gccgcatcgg gcgttttgcc ttttttcagt
    10561 ccggcttcga gtttgtcgga tgcaagtttc aagagtctgt cgtgtgtgtc tttgtccaga
    10621 aagggcagtt gtccggcggc ggatttttgt gccagttctt taaaaaggtt gccgaagctg
    10681 tttttgcggt aactgttgcc gttgaggtcg ggatgaatgc gccaaaagcc ggcttccagt
    10741 tctggcagca ggcggcagat ggtttgccat gaggtttcgg cgttgcgctg cgcctgtttc
    10801 aaatccgcct gcggacggcg gaaattcagg tacgggcggg aaagatagcg cgaaatttgg
    10861 gcaaggacgg tttgcggcac agctttgcgt ttaagcgcca atgcggcaag caccggatca
    10921 ttgctgacgc gttcccgcca aaaatcttgc gccgggataa gcaggcggtc gccgtcttct
    10981 tcggtcattt cgacatcgaa cggtgcttgg cacaggaagg cgtagtcgcg caggatgcgc
    11041 tggcagaagc cgtggatggt atagatggcg gcgttgtcga attgcccgat ggcggccttg
    11101 aggcggacaa tcagacgcgt ccggccctct ttttgcaaag cctgttttaa gagttcgggc
    11161 aggaaggtgt cgccttcgtg gtgttcggcg cagtaggcgg caatgccgtc tgaaagcgtg
    11221 tcgtctccaa gtttggcaat tcctttgctt tctaaaactt gtaacacatc gtccaaacgc
    11281 ccgcgcaggc gtgttttcag ctcggcggtg gcggctttgg tgaaggttac gaccaatacg
    11341 cgttcgacgt ttttttgttc taatacgatc aggcgtgtaa acagggcggc aatgccgtag
    11401 gttttgccgg tgccggcaga ggcctcaatc aggttggtgc cggaaatggg gacggttagc
    11461 gggtcgaatg cttggatgct tgcagacata gtgcgcgctc ggaaaacggt tggacg
//
LOCUS       DAWFAN010000057        11498 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWFAN010000057 DAWFAN010000000
VERSION     DAWFAN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.057"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            271..1107
                     /locus_tag="WGO65_001051"
     CDS             271..1107
                     /locus_tag="WGO65_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ0327354.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLDLRLINGVEISVTWRGRTIHVVGLDFDERDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RY"
     gene            complement(1159..2451)
                     /locus_tag="WGO65_001052"
     CDS             complement(1159..2451)
                     /locus_tag="WGO65_001052"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ0327355.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHIEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(2507..4417)
                     /gene="pglD"
                     /locus_tag="WGO65_001053"
     CDS             complement(2507..4417)
                     /gene="pglD"
                     /locus_tag="WGO65_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ0327356.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFRILSTALAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEV
                     VKQMREYSAAAFVDDDPKLWHTVICGLAVYRPDDISALIERYGVEKILLAIPSATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLN
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVRTFVLISTDKAVRPTNTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHQDITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWRELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            complement(4465..5640)
                     /locus_tag="WGO65_001054"
     CDS             complement(4465..5640)
                     /locus_tag="WGO65_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ0327357.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPTTKAVIVVHLAGMPAEMDGIMALAKEHNLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVSELNARKVPCYQGSCSEV
                     YLEKSFDDTPWRPKERLKNAVELGDTSLMFLVHPTLTDDEIAFCKEHIEAVLAEATR"
     gene            complement(5718..6959)
                     /locus_tag="WGO65_001055"
     CDS             complement(5718..6959)
                     /locus_tag="WGO65_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEZ0327358.1"
                     /translation="MSKFFKRLFDIVASASGLIFLSPVFLILIYLIRKNLGSPVFFFQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDGERLTPFGKKLRAASLDELPELWNILK
                     GEMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNAASWDERFACDIWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPTFTGKRKLAVVGAGGHGKVVAELA
                     AALGTYGEIVFLDDRAQGSVNGFSVIGTTLLLENSLSPEQYDIAVAVGNNHIRRQIAE
                     KAAALGFALPVLIHPDATVSPSATVGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESWIGTGACSRQQIRIGSRATIGAGAVVVRDVSDG
                     MTVTGNPAKPLAGKNTETLRS"
     gene            complement(7127..8548)
                     /locus_tag="WGO65_001056"
     CDS             complement(7127..8548)
                     /locus_tag="WGO65_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ0327359.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANTPPARLSATAESASALLAAAVCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFAFKTESSCRLWQPLKRLPLYLHTLFCLASSAAYTCFGTPANYPLFAGIWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            complement(8743..9852)
                     /gene="ribD"
                     /locus_tag="WGO65_001057"
     CDS             complement(8743..9852)
                     /gene="ribD"
                     /locus_tag="WGO65_001057"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ0327360.1"
                     /translation="MFSDTDISMMETALRLAALGRFSTSPNPRVGCVIAHGSQVVGQG
                     FHVKAGEPHAEVHALHQAGEMAQGATAFVTLEPCSHYGRTPPCAEALVRAGVSRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYRKHAHVRILMPSETAGGK
                     IDLNSLMRLLADEGIGELIVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLFSLP
                     ENRAALSAPPLWMPVSTEILGHDIKTVFRKNGNAF"
     gene            complement(9885..10349)
                     /gene="nrdR"
                     /locus_tag="WGO65_001058"
     CDS             complement(9885..10349)
                     /gene="nrdR"
                     /locus_tag="WGO65_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ0327361.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPSCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            complement(10378..11220)
                     /locus_tag="WGO65_001059"
     CDS             complement(10378..11220)
                     /locus_tag="WGO65_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ0327362.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT         2349 a         2949 c         3657 g         2543 t
ORIGIN      
        1 cacgttttca gacagtcttt tatagtagat taaaattgaa aacggtcata tcgccattcc
       61 ctcgaaagca ggaatccgga agtttgaaat agcggttaac cttaaacatt tccaataaat
      121 caaagtctgg attcccgcct gcgcgggaat gaccgcatag cggcttttcc tttgcatttg
      181 ccatacacct gttttttgaa atcaccgccg ccacaccgcc cgacaatatt gaaacaccct
      241 gcccaccatt taccacgcac ataacgcctt atgatagacc tgcactgcca ttccaccgtt
      301 tccgacggta tgctctcccc cgccgaagtc gtccgcctcg cgcatcaaaa cggctgcacg
      361 ctgctggcgt tgaccgacca cgaccacacc ggcggcatcg ccgaagcgcg tgccgaagcc
      421 gacaggctcg atttgcgcct gattaacggc gtggaaatct ccgtcacttg gcgcggacgc
      481 accatacacg ttgtcggttt ggatttcgac gaacgggacg aaaacctgca aaacctgttg
      541 gcgcaagtgc ggcaaggccg tctgaaacgc ctcgaagcca tcgccgccaa gcttgaaaag
      601 aaaggcatcg gcggcgcata cgacggcgcg ctcgcgctgg cggcaaacaa agaaatggtc
      661 agccgcacac acgtcgccga gttcctcatt cgggtgggac acgtcaaaaa caagcagcag
      721 gcgttcacca aatacttggg cgacggcaaa tcctgcgccg tccgccacga atgggcgacg
      781 ctggaagact gcgtctccgc cgtcaacggc gcgggcgggt tggcggtcat cgcccatccg
      841 atgcgttatg atttgtccgc caccgccaag cgcaatctgt ttgaagaatt taaaaacctc
      901 ggcggcgcag gcatcgaagt ccacagcggc aactgctgca aaaacgaccg cctcaactac
      961 gcgcttttgg cagaacgctt cggtatgctc gccagcgcgg gcagcgactt ccaccgttta
     1021 aacgacttta gcggcggcat tctcggcgcg tgtcccgaac tgccggaaaa ctgcaggccg
     1081 gtgtgggcgc atttttcccg atattgaatg cagataaaaa tgccgtctga aaacctttca
     1141 gacggcattt tttgcggttt aaacgttgtc gtacagtttt cggacggttt tgccgatggc
     1201 ggcgatgcct ttttccagcg tttgagcgtc ctgcgcgatg ctcatgcgga tgcactcgcc
     1261 cgcgtgcggg taatcccgcg tgtcgatgcc gacgaagaaa tgctcgcccg gaataatcag
     1321 cgtaccttcg gctttgagca tttcgtacag ggtttgcgaa gaaacgggca ggttttcaaa
     1381 ccagagccac aggaaaatcg cgccttcggg tttgtggatt ttcatcgggt acgcgcccag
     1441 ctcgcgcttg agcagcgaga cggcggtttg cgcctgattg cggtaaaacg gccggatgac
     1501 ttggtcggca agccgtttca tctcgccgct ttccagcagc ggggcggcga tggccgcgcc
     1561 gaagcgcgtg ggggcaaggt tcacaatcgc gttcaggctg ctgacggctt tgacgacttc
     1621 gggcgcggcg acgatgatgc cggtgcgaac gcccggcagg ccgactttgg aaaggctgaa
     1681 gcagaggatg atgttttcgt gccaattcag cgttacgtcg ctgtaaatga tgttggggaa
     1741 cggcattccg taggcgttgt cgataatcag cggaatcccg tgttcacgcg ccaaagcgtc
     1801 caaacgtgcc atttcgccgt cggtcaacac attgccggtc gggttggtcg ggcgcgaaca
     1861 gcaaatcgcg ccgattttgc ccgctttgag ttcgggcagg ttttccagtg cgtcaaagtc
     1921 cacgcggtat ttgaagaagc ctgcttcgcc ttcgtgttcg acgttttcga ttttgggctt
     1981 gacagaaacg aagtgctgcc cttcgatatg tacgtcggca tagccgatgt attcgggcgc
     2041 gagcggcaac aaaatggctt tttctgcgga tgtgccgtct gaaagcttga atttgccgcc
     2101 gaagaggttg aataaataga aaaacgcgtt ttgcgaaccg ttggtcagcg cgatattgtc
     2161 ggcggtcagg ttccagccgt attcgcggtt gaggaaggcg gtcaacgcgt caatcagcgc
     2221 ggcatcgcct tgggggttgg agtagttgcc gatgttttcg acggcgtgtt ctgccgccag
     2281 tttggagaat atgtcggcaa acgcccgatc gatttccgga atgcgcgccg ggttgccgcc
     2341 gccgagcatg ttgacgggct tgtcgctttt gagcgcgtcg ccgaggtcgt ccatcagttg
     2401 gaggatgccg ctgtgttgcg tgaatttttc gccgaatgcc gaaaactgca tgtcaaactc
     2461 cgtgtgtgta aggtaggttg gtttgtacgt tatgccgtct gaaggttcag acggcatttt
     2521 ttctgtgtgt ttctcgtacc caaagcaggt cgcagatacc gtcgctcggg gcaaagccgg
     2581 tcggggcgtt gatgagcagg gtgcggattg cctgctggtc gtaacggtcg caggccgcac
     2641 ggatgcggtc gagcagggcg gagagctcgc gccacggcag catggtctcg ttggcggtca
     2701 tgatgcgcgg atggccggtt ttgcggacgt tgtcgccgat gagcagctct tcgtagagtt
     2761 tttcgccggg acgcagtccg gtaatgagga tttcgatgtc gccgtcgggt tgttcgggtg
     2821 ttttgggttt gaggccgctt aaggtaatca tttggcgggc aaggtcgatg attttgacgg
     2881 attcgcccat atcgaggacg aatacgtcgc cgcccgtacc catcgcgccg gcttggataa
     2941 ccagttgcgc cgcttcggga atggtcatga agtagcgggt aatgtcttgg tgggtcaggg
     3001 taatcgggcc gccttctgca atctgttttt caaacagcgg gacaacggag ccggacgaac
     3061 ctaaaacatt gccgaaacgc accatgctga agcgggtttt ttgtccgggt tcggcggcga
     3121 gcgcttgaag gcagagttcc gccatgcgtt tgccggcacc catggtgttg gtggggcgga
     3181 cggctttgtc ggtggagatg aggacgaaag ttcttacgcc cgatgccgtg gcggcaagcg
     3241 cgcactcgag tgtgccgaag atgttgttgc gtatgccttc gacggtgttg aattcgacca
     3301 tggggacgtg tttgtaggcg gcggcgtgat agacggtcgc aacggaaaag gcggtcatga
     3361 cgtgttcgag cagggtgcgg ttttgcaccg aaccgagaaa gggcaggatt tcggtgttga
     3421 ggcgtttttg gatgcaggtt tcgcgcaatt ctttttcgat ggcgtacagg gcgaattcgg
     3481 ataactcgaa cagcagcagc ttttcggggc ggcggcggat aatctggcgg cagagttccg
     3541 aaccgatgga gccgcccgcg ccggttacca tgacggtttt gccttcgatg tcggcactca
     3601 tgaggcggtt gtcgggcgcg acggaatcac gcccgagcag gtcggacacg gagatttttt
     3661 tgagcgtgcc gatgctgatt ttcccgtcca tcaggtcttt cattccggga atggtcaaca
     3721 cttcgcaggg ataggcttcc agtttgttga tgattcggcg gcgttgttcc tgagtcgcgc
     3781 tgggaatggc aagcaggatt ttttccacgc cgtagcgttc gatgagggcg gagatgtcgt
     3841 cggggcggta aacggcaagg ccgcagatga cggtgtgcca cagtttgggg tcgtcgtcca
     3901 caaaggcggc ggcggaatat tcgcgcattt gtttgacgac ctcgagaagc tgtctgcccg
     3961 aacgccccgc gccgtaaata atgacgggga tcatctgttt tttggggtgt tcggacaaca
     4021 gtccgcgcaa aaccatacgc gagccggtca cggaaacaaa cagcagtaag aaatagacaa
     4081 tcggcagggc gaggcgcagc ctttcttcaa aaatcaatgt gttgaggaag aacaggacgg
     4141 cggaaacaat gctgcctgcc agcgcggtgg agaggatgcg gaagctgacg aagcgtgtaa
     4201 cggcgcggta aagccccatt cggataaata atgtgatggt cagcaaggca gtcagcaaaa
     4261 aagactgcca gttggcaaaa tcgaaccatt cgtccgagta gtcggccttt aggctttggg
     4321 tgaaccaaaa ggcaatgaaa atcatcagaa aatcgtgtat gaggaaacag attttcttga
     4381 tgttgcgcgg cagggcgatc agggtttcca gagtcatgtt gtcggcggcg tgtatttttc
     4441 agacggcata tgccgtctga atggttatcg tgtggcttct gccaatacgg cttcgatgtg
     4501 ttctttgcag aacgcgattt cgtcgtcggt cagcgtcggg tgcaccaaga acatcaggct
     4561 ggtgtcgccc agttcgacag catttttcaa acgctctttc ggccgccaag gcgtgtcgtc
     4621 gaaagatttt tccaaataga cttcggagca gctgccttga tagcagggga ctttgcgcgc
     4681 gttcagttcg ctgacgatgc ggtcgcgcgt ccagccgtct ttgaggtgtt cgggtttgac
     4741 gaaagcgtag aacttatatt gcgcgtgtcc gatgtagtcg gcgacttcaa ccaagcggat
     4801 acttttgaat ttgcctaggc tttccgccag cttggcggcg tgcgcttgac ggcaggcagt
     4861 ccattcgggc aggcgtttga gctggatgcg cccgattgcc gcctgcattt ccatcatacg
     4921 ccagtttgtg ccgaaacttt cgtgcagcca gcggaaaccg ggcgcgtgtt cgcggtggta
     4981 cacggcatcg tagcttttgc cgtggtcttt gtacgaccac attttttccc acagggtttt
     5041 gtcgttggtc gtaaccatgc cgccttcgcc gcccgtggtc atgattttgt cttggcagaa
     5101 cgaccatgcg ccgacgtgtc cgatgctgcc gacggatttg cctttgtatt tcgcgccgtg
     5161 cgcttgggcg cagtcttcga tgacccaaag attatgttct tttgccaaag ccataatgcc
     5221 gtccatttcg gcgggcatac cggcgaggtg gacgacgatg acggctttgg tagtcggtgt
     5281 cagcgcggct ttgacggttt ccgcgctgat gttttggctg ttcaaatcca catcggcaaa
     5341 caccggattt gcgcccgcgt tcacaatgca ggacgcggaa gcgaggaagg tgcgcgaggt
     5401 aacaatcaca tcgtcgcccg cgcctatgcc cattgctttg agcgcgacat cgagtgccag
     5461 cgtgccgttg gcaagggcga cggcgtaccg cgtgccggca aaggcggcaa attctttttc
     5521 aaattcgcgg cattcgttgc ccgtccagta gttgactttg ttggacagca ggactttgga
     5581 aacggcatcg gcttcttctt gggtgaagca gggccacggg gaaaggaaag tgttcagcat
     5641 gatggtttgt ccgtcggttt tcagacggca tttcctcatt tctgccgtct gttgcttagg
     5701 ctgtttattt taatcgttta cgaccgcagg gtctcggtat ttttgcctgc caatggcttt
     5761 gccggattgc ccgtgacggt catgccgtct gaaacgtcgc gcacgacgac tgcgcccgct
     5821 ccaatggttg cgcggctgcc gatacggatc tgctggcggc tgcacgcgcc tgtgcctatc
     5881 cagctttctt cgccgatacg cgtgttgccc gacaggtgcg cgcccgggct gatgtggacg
     5941 aaagcatcaa gcaggcagtc gtgatcgacg gtggcggcgg tgttcacaat cacgccgtct
     6001 ttcaatacgc tgccagcctg tacgacggct ttcgccataa cgacgctgcc ttgtccgact
     6061 gttgcagaag gcgagacggt cgcgtcggga tgaatcagaa cgggtagggc gaagccgagc
     6121 gcggcggctt tttcggcgat ttggcggcgg atgtggttgt tgccgacggc gacggcgatg
     6181 tcgtattgtt cgggcgataa actgttttca agtagcagtg tcgtgccgat gacggaaaag
     6241 ccgttgacgc tgccttgggc gcggtcgtcc agaaaaacga tttcgccgta tgtgccgagt
     6301 gcggcggcaa gctcggcaac gactttgccg tgtccgcccg cgccgacgac ggcaagtttg
     6361 cgttttcctg tgaaagtggg catggtagct tcgccctgtg ctgaaatccc ttccttgatt
     6421 aatacttttt tgactgtcag cagtaggatt ttgatgtcga ggcacaggct gaagtggtcg
     6481 atataccaga tgtcgcatgc gaagcgttcg tcccatgagg cggcgttgcg cccgttgacc
     6541 tgcgcccagc cggtaatgcc cggtttcatt tcgtggcggc ggttttggaa gttgtcgtac
     6601 agcggcagat attgcatcag cagcgggcgc gggccgacca ggctcatctc gccttttaag
     6661 atattccata attcaggcag ttcgtccaaa ctggcggcac gcagtttttt gccgaacggt
     6721 gtcaggcgtt ctccgtcggg cagcggaatg ccgtctgaat caagcgcgtc gcgcatggaa
     6781 cggaatttga ccattttaaa aggttttccg tcctttccgg ggcgttcctg aaagaagaag
     6841 acgggcgaac ctagattctt gcggatgagg tatatcaaaa tcaaaaatac tggcgagagg
     6901 aaaatcagtc ccgaggcgga ggcaacaatg tcaaacaggc gtttgaagaa tttactcatg
     6961 ggctaatctt tcaatcaggt tgacgatttt ccgataggaa atgtcgcgcc tgaagcggcg
     7021 gacgatttcg tcggactgaa cggggtcgtt tttgcacgcc atttggcaat cgatgatatc
     7081 gtgcccgtgc cgcccaagcc tgccatagat gtggtcaaaa cgattttcat aatgggaaac
     7141 cttgtttttt caaataatga aacagtttgt gcaaatcttt ccggtggcgc aggatgcagc
     7201 ctgccagata tgccgcccat atgccggcaa acagggggta gtttgccggc gtgccgaagc
     7261 aggtgtaggc cgccgaggag gccaggcaga acaatgtgtg cagataaagc ggcaggcgtt
     7321 tgagcggctg ccacaggcgg caggagcttt cggtcttgaa ggcaaaaaac agccagaatg
     7381 aggcggcaca ggcaaccgcc gcgccgcgcg cgccgccgga cggtacggca agccccagca
     7441 gcagcaggtt tgccgccagc gcgcccaagg tggcgagcgc gatcgggcgt gtttttcgga
     7501 cgacgttcaa accgatgccg ctgatttcta ccagcgtgca aaacagcggc ggcagcatac
     7561 acgatacgac gataaaccgg acggcggcgt agttttccgg cagcaggagg gaggcgaggg
     7621 gcgagaaaat gccggtcagg cagacggcgg cggcaagcag ggcggaggca gattctgccg
     7681 ttgccgagag gcgggcgggc ggggtgtttg cttcgattgc gcggaaaata tacggtgtcc
     7741 agaccgttga aaagatgctt tggaacaata atgccgcccc gccgaacgaa atacccatcg
     7801 aataaacgcc gagctgttct aggccggcat attttttcag gaacaaacgg tcggcggatg
     7861 ccagccccca ataggcgatg ctgcttagtg cgatcggtat gccgtagcgc aggccgcgat
     7921 gcaggacggc ggatgaaaac ggtgcgcgcc ggacggcctt cagacggcat cggttttgaa
     7981 acagcaaaaa ggcggcggcg gcaaggtttg ccagcgcgta aacggcggtc aggacggcgg
     8041 tgttcgccgg aaagtgcagc agcccgaccg tcagcggcag cagcagcagg atggcgagct
     8101 tggacacgag ttgcgcggac gaaaaggcaa gggcgcgccc ttccatacgc aaaaccagta
     8161 agagaaagcg gatgggcagg aagctcagtt caaacagcac cagcccgatg ccggcggcgg
     8221 catcgtcgag cgaaaacagg atttcagacg gcagggacgg gcgggaaagc agcagggcgg
     8281 ctatcgcggc ggcagacagc agcggcggca ggaacagggt tttgaacaag gtgtctttgt
     8341 cggcggcggc atagtattcg cggacgtatg cctgatccag cccgaggcac aataccgaca
     8401 cagtcagtcc cgccgccgtc tgcatcagca cgatgcgccc gatgtcgtcg gcggggaaat
     8461 accacgacag cagcggcagg atgatgacgg ctaaaaccgc gctgccgatc gagcctgccg
     8521 cgtagccgag gatttctttt gtgtccattt ttgatgtccg ggcggcggcg ggatgctgcc
     8581 tgtgtcgtct gaagcctttc ttgatcggaa tttgacggct ttcagaccgt cgcggctgcc
     8641 ggcggggtgc ggcagtccgg gtcgcgcttt tccgggcggg cggcggtctg aacggggccg
     8701 ttttttatcg gcgttattat atagtgaaac ggcgcaaacc ttttaaaagg cgttaccgtt
     8761 ttttcggaac acggttttga tgtcgtgtcc gaggatttcg gtggaaacgg gcatccacaa
     8821 gggcggtgcg gaaagggcgg cgcggttttc gggcagggaa aacaggtctt tgccgctgcc
     8881 gaggattttg ggcgaacggt acagcacgat ttcgtctgcc aaatcttctg ccaaaaatgc
     8941 agatgtgagt tcggagcctg cttcgactat gagttcccct atgccttcgt cggcaagcag
     9001 gcgcatcagg ctgttcaaat cgattttgcc gcctgccgtt tcagacggca tcaggatgcg
     9061 gacgtgggcg tgtttgcgat aggggcggag tttgtcttca tcgcgttcca aggtggcgat
     9121 gtaggttgga gattgtccgt cggtcagaag gtggctgtcc aagggcaggc gcaggcggct
     9181 gtctaaaacg atgcgtgcgg gttggcgcaa agttggaaaa gcgcggacgt tgagccgggg
     9241 attgtccgcc aacaccgtgc cgatgccggt cagcaccgcg cagctttcgg cacgcaaaac
     9301 ctgtacgtcg gcacgcgcgt cttcgccggt aatccaaaag ctgctgccgt ctgaaagggc
     9361 ggttttgccg tccagcgaaa cggcgcattt gaggcggaca aaggggcggc ggcgttcgat
     9421 gcgcgacagg aagcctcggt tgagttccct tgcctgatgt tcgagtaaac cgcattccgt
     9481 cttgatgcct gctgcttcga gcagggcaag ccctttgcct gcaaccagcg ggttggggtc
     9541 gcgcatggcg gcaacgacgc gggacacgcc cgcccgcacc agtgcttcgg cgcagggcgg
     9601 tgtgcgtccg taatggctgc atggttcgag ggtaacaaag gcggtcgcgc cttgagccat
     9661 ttcgcccgcc tgatgcaggg cgtggacttc ggcgtgcggt tcgcccgctt tgacgtggaa
     9721 gccctgcccg acaacttggc tgccgtgtgc gataacgcag ccgacgcgcg gattgggcga
     9781 agtggaaaaa cgccccaaag cggcaagtcg gagggcggtt tccatcatgg atatatctgt
     9841 gtccgaaaac atggggatac cgtatcagta tgggttgggg agaatcaggc tttgcggtct
     9901 gttttgacgg cttgcgccag ccacgaggca aagtctgccg gattgtcgaa gcgtttgtgc
     9961 agggcggcga aacggacggc ggcttccgta tcttgtcgga gcagttcttt tagcaccatg
    10021 tcggcaagtg cggcagaggg gatgtcgcgc tgacccgaag tgtagagcct gtgttccgtc
    10081 aggcggacgg tttcgtcgat ctgttcgggt gtcagggcgg attttcgggc ggcggcggtc
    10141 aggtcgttgc ggaggcgttg tgcattaaag ggcgagcgtt ttttgtccgg accgatgacg
    10201 gcgggcattt tgagttcggc ggtttcgagc gtgccgaagc gtttgccgca gctggggcag
    10261 tggcggcggc ggcgcacggc gttgcgttct tccatcaggc gcgaatcggc gacgcgggtg
    10321 tcggggtggg cgcaaaacgg gcatttcata gcgtttcttc ctgatgccgt ctgaacgtca
    10381 aaccgatacg ccggcggcgc gggcgatttc cagaccttct tcggcactca tatagacggg
    10441 gttttcggga cggtcgtgtc ggacgatgtt gccttcgcgg aacatgacca gttcgtccac
    10501 ggcaagttgg gaccatgatt cgtcgcgggt cagcggcagg gtggcgataa cggcgacgcg
    10561 gtcggagggc gtggttactt cggcaaaatc gaccatcacg tcgtcgtcga gcaggcgcgc
    10621 cttgccgaac ggggcttggc ggacgatgta gtgcagcagt gtgctggcgt gggcaaacag
    10681 ggcaatgccg tctgaaagca taaagttaaa cagcccgaac ttgcggattt cgtgcgtcag
    10741 ccccgcaatc gcgtcaaaca gcgtgtcgtc gtcgggacgg gcggcaaagc gggtgcgcag
    10801 gcggttgagg atgtggcaga acgcgcgttc ggaatcggtt gtgccgacgg ggtggaaaaa
    10861 ttcgccctgt tcgggaaaaa aatcaatcaa atgtccgttg tgggcaaaca gccagtagcc
    10921 gccccacatt tcgcgcataa agggatgggt gttcgccagc gaggtttgtc cttgtgatgc
    10981 tttgcggata tgggcgacga cgttttccga tttaatctgg taggcgcgca cgaggtcggc
    11041 gacgggcgaa ttggcactgg gtttgtcgtc atggaacagg cgcacgcctt tgccttcgaa
    11101 aaagccgata ccgaaaccgt cggcgtgatg gtcggtaatg ccgccgcgcc ggcggaagcc
    11161 ttcaaaggaa aacataatat cggtcggcgt attgcagttc atgcccagca gttgacacat
    11221 ggtttgtcca aatgattcag atggtcgcaa gtattcggat tataccccga actgaaaatg
    11281 ccgtctgaaa tacggcttgt tccccattat tccctcgaaa acagaaaaca aagacggaaa
    11341 cttaagattc cgtcattccc gcgcaggcgg gaatctagaa tttcaatgtc tcaagaattt
    11401 atcggaaaaa accaaaaccc ttccgtcgtc attcccacga aagtgggaat ctagaaatga
    11461 aaagcaacgg gaatttatcg gaaacgaccg aaaccgaa
//
LOCUS       DAWFAN010000058        11181 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWFAN010000058 DAWFAN010000000
VERSION     DAWFAN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.058"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(15..218)
                     /locus_tag="WGO65_001060"
     CDS             complement(15..218)
                     /locus_tag="WGO65_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327363.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(196..429)
                     /locus_tag="WGO65_001061"
     CDS             complement(196..429)
                     /locus_tag="WGO65_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0327364.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            962..4396
                     /gene="dnaE"
                     /locus_tag="WGO65_001062"
     CDS             962..4396
                     /gene="dnaE"
                     /locus_tag="WGO65_001062"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ0327365.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     EAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELSLPVVATHPTQFMSSDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQSNRGRVIEYVREKYGAKAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITSARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMNNADQKAANTNQGGL
                     FDMMEDAIEPVRLVDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLS
                     SHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            4674..7922
                     /gene="cas9"
                     /locus_tag="WGO65_001063"
     CDS             4674..7922
                     /gene="cas9"
                     /locus_tag="WGO65_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ0327366.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA
                     DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNI
                     NIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     gene            7989..8903
                     /gene="cas1"
                     /locus_tag="WGO65_001064"
     CDS             7989..8903
                     /gene="cas1"
                     /locus_tag="WGO65_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ0327367.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAKHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            8896..9222
                     /gene="cas2"
                     /locus_tag="WGO65_001065"
     CDS             8896..9222
                     /gene="cas2"
                     /locus_tag="WGO65_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ0327368.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     repeat_region   9286..10575
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9286..9321
                     /rpt_unit_seq="attgtagcactgcgaaatgagaaagggagctacaac"
     gene            11015..11181
                     /locus_tag="WGO65_001066"
                     /pseudo
     CDS             11015..11181
                     /locus_tag="WGO65_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         3173 a         2957 c         2675 g         2376 t
ORIGIN      
        1 aagccgaacc gttttcagac ggcatcgtcc gaccaacccg cttctttcaa tttccgccgt
       61 tgcgcgtcgt agcgggcctt atccgcccaa taaatcgtct gaatacacgc ctcgccgcag
      121 tcgctgccgc agcattccca cgactcgggt cggacgggtt cgtctaaaag cggctcgccc
      181 aaaagggctt cggctttatg cttcagggtc atatccatcg gcgatttcca agcgcgcacc
      241 gtcaaactcg atgacttcgc cgccgcgtat tttggcggtt ttacgggttt cggtttcgcc
      301 gttgcgcgac accaaccctt cggcaatcaa cgccttcgcc tgtccgccgc tttcggcaag
      361 tccggccaat ttcaagaggt cgcacagggc gatgtattcg ttgtcttcga gatagacggt
      421 ggcttccata atgttccctt gcagaaagag gccgttattg tagcacctgc cgccgccgta
      481 cccaaaatta ccgcaaaaca ggcagcacat cccgccccgc ctgttccgta aaaatgccgt
      541 ctgaaacccc atatgccgtc atccgatcaa agaaatcctg tccaacggca gactgcaaat
      601 cctgttcccc gacgaatccg cattgacgct gatgcacatc ctcaaacgcg aactgcccga
      661 tacaccggca atcggcatca aaacgaaatc aaaaacctgt ttgaacgtaa aattaattta
      721 actgcttgcc atccgtccga ataaggtata tagttcctta taactagttt gatagtcctt
      781 tatatctatc aatactcctt gggaagcctc cgccatacgg caggaggcat ttttttgccg
      841 ccgcaaaagc tcaaaaacat ttgcaggtca tgccgtctga atccgaaaca gcattaccta
      901 caccgccatc taaagacaac cctgctacaa tacgcctttt attgtccacg ccgattttgc
      961 catgaccgag ccgacctaca tccccctgcg cctgcatacc gagttttcga ttaccgacgg
     1021 tatggtgcgg attaaaaaac tgattgccaa agcgcaggaa tacggtttgc ctgctttggg
     1081 catcagcgat ttgatgaacg aattcggctt ggtgaaattc tataaagcct gccgcagcgc
     1141 aggaattaag cccatcggcg cggcggacgt gtggataggc aatccgaatg cgcccgacaa
     1201 gccgttccgt gccatgctga ttatccgcaa cgatgcgggc tatctgcgtt tgagcgagct
     1261 tttgacagag gcctatgtcg gcaaagaccg caatatccat catgcggaac tcaaccccga
     1321 atggctggaa aacggcgaca acagcggctt gatttgtttg agcggcgcgc attacggcga
     1381 agtgggcgtg aatctcttga acggcaatga agacgcggcg cgtgcggcgg cgttgaaata
     1441 tgcggcatgg tttcccgatg cgttttattt agagctgcaa cgcctgcccg aacgccccga
     1501 atgggaggct tgcgtttccg gcagcgtgaa gctggcagag gaactgagtt tgccggtggt
     1561 ggcgacccac ccgacgcagt ttatgagcag cgatgatttc aacgcgcacg aggcacgggt
     1621 gtgtattgcg ggcggctggg tgctgacgga caaaaaacgt ccgcgcgatt tcacaccggg
     1681 ccagtttttc attccgccgg aaacgatgct ggagcgtttc gccgatttgc ccgaggcctt
     1741 ggaaaacacg gtagaaattg ccaaacgctg caatctgcac atcacattgg gcaaaaactt
     1801 cctgcccctt ttccccacgc ccgacggttt atcgcgcgat gactatctca tcaaactctc
     1861 aaacaagggt ttgcaggaac gcatggttca gctttatccc gacgaggcgg agcgtgcggc
     1921 aaaaatgccg gaatatcagg aacgtttgga ttttgagctg aacatcatca tccagatgaa
     1981 attccccggc tatttcctta tcgtacaaga ctttatcaac tgggcgaaaa cgcacggctg
     2041 tccggtcggg ccgggacgcg gttcgggcgc gggttcgctg gtggcgtatt cgctgaaaat
     2101 taccgacctc gacccgctga aatacgcgct gctgtttgaa cgtttcttaa accccgaacg
     2161 cgtctccatg cccgactttg acgtggactt ttgccaatcc aaccgggggc gcgtgattga
     2221 gtatgtgcgc gaaaaatacg gcgcgaaggc agtcagccaa attgtcacct tcggcacgat
     2281 gtcgtccaaa gcagtgattc gcgacgtggg gcgcgtactg gaactgccgt ttatgctgtg
     2341 cgacaaactg tccaagctga ttccgttgga agccaacaaa cctctgggtt tggacgacgc
     2401 gatgaaggcg gagccgcaga ttcaggaatt aatcgaagcg gaagaagcgg acgaactgat
     2461 tacgctggcg aaaaagctgg aagacctgac gcgcggtttg ggtatgcacg caggcggcgt
     2521 gttgattgcg ccgggcaaga tttccgatta cagccccgtg tatcaggcgg atgaatccgc
     2581 ctctcccgta tccatgtacg acaagggcga cgtggaagac gtgggtttgg tgaaattcga
     2641 ctttttaggt ctgcgtaacc tgaccattat cgaaatggcg cagaacaaca tcaaaaacac
     2701 caccggcgac atcatcgatg tcggcaaaat tccgctcgac gaccaggccg cctaccaaat
     2761 cttccgcgat gcgaacacca ccgccgtgtt ccagttcgag tcgaccggca tgaaaaaaat
     2821 gctgaaaacg gcgcatacca ccaagtttga agaactgatt gccttcgtat cgctctaccg
     2881 cccaggcccg atggacaaca tccccgactt cgtcgcgcgt atgaaggggc aagaattcca
     2941 atacatccat ccgctgctcg agggcatcct cgcgccgacc tacggcatta tggtgtatca
     3001 ggaacaagtg atgcaggcgg cacagattat cggcggctac tcgctcggcg gcgcggacct
     3061 gctgcgccgc gcgatgggca agaaaaagcc cgaagaaatg gtgaaacacc gcgaaatctt
     3121 cgccgaaggc gcggcaaaac aaggcatttc gcgcgaaaaa tccgacgaaa tcttcaacta
     3181 catggaaaaa ttcgccggct acggtttcaa caaatcccac gctgccgcct acgccctgat
     3241 ttcctaccag accgcatggc ttaaagccca ctatcccgcc gaatttatgg cggcgaccat
     3301 gtcgtccgaa ttggacaaca ccgaccagct caagcatttc tacgacgact gccgcgccaa
     3361 cggcatcgag tttctgccgc ctgacatcaa tgaatccgac taccgcttca cgccgtatcc
     3421 ggacatgaaa atccgctacg cgctcggcgc gattaaaggt acgggcgaag ccgccgtcga
     3481 atccatcacc tccgcgcggc aaagcggcgg caagtttacc ggcctcttgg acttctgcga
     3541 gcgcgtcggc aaagaacaca tgaaccgccg cacccttgaa gccctgatac gcggtggcgc
     3601 gttcgacagc atcgaaccca accgcgccat gctcttggcg aacatcgacc tcgccatgaa
     3661 caacgccgac caaaaagccg ccaacaccaa tcagggcgga cttttcgaca tgatggaaga
     3721 cgccatcgaa ccggtgcggc tcgtcgacgc gccgatgtgg agcgaatcgg aaaaactcgc
     3781 cgaagaaaaa accgtcatcg gcttttacct gtccggccac ccgttcggcc cgtatgccca
     3841 agaagtccgc caaatcgccc cgcagaaact gtcgaaactc aaaccgcaag acagcgtgcg
     3901 cctcgccgga ttcgttaccg ccgtgcgtac gatgatgggc aagcgcggca aaatcgcctt
     3961 cgtcagcctt gaagatttga gcgggcaggt tgaaatcatg gtcggcggtc agacgctgga
     4021 aaactgcgcc gactgcctca aagccgacca agtgctgatt atcgaatcca aagtcagccg
     4081 cgacgactac ggcggcggcg acggactgcg catcatggca aaccaagtca tgaccctgca
     4141 aacggcacgc gaacgctacg cccgcagcct cagcctcgcc ctcgccccgc atcacgacat
     4201 cggcggactg gtacggctgc tctcctccca ccaactgccc gacacgccgc gcatcccgct
     4261 gcaactgtcg tatgccaacg aaaaagcgtc gggcaggctt caagtgccgc cgaaatggac
     4321 ggttacaccg agctccgcat tgttcggcga actggaaaca ttgctcggca gccggtcggt
     4381 gcgcgtcaac tggtaaccca aaatataaat gccgtctgaa gcccaaaatc ggtttcagac
     4441 ggcatttttt aaccgaaata aacgatgccc cttaaagcag aagctttaag gggcagagcg
     4501 ttgcggcaca tcttttcaga cggccttatt gtagcaacgg ttctcatttc gcagtgcgac
     4561 aatatgtttg ggattctagc cgttgtgaga tgataagtca ataatctatt tgtatataaa
     4621 taagacaata agatatatta tcattatttg caacgttgtt tacggagtaa taaatggctg
     4681 ccttcaaacc taatccaatc aactacatcc tcggcctcga tatcggcatc gcatccgtcg
     4741 gctgggcgat ggtagaaatt gacgaagatg aaaacccaat ctgcctgatt gatttgggcg
     4801 tgcgcgtatt tgagcgtgcc gaagtaccga aaacaggcga ctcccttgcc atggcaaggc
     4861 gtttggcgcg cagtgtccgc cgcctgaccc gccgtcgcgc ccatcgcctg cttcgggccc
     4921 gccgcctatt gaaacgcgaa ggcgtattac aagccgctga ttttgacgaa aacggcttga
     4981 tcaaatcctt accgaataca ccatggcaac ttcgcgcagc cgcattagac cgcaaactga
     5041 cgcctttaga gtggtcggca gtcttgttgc atttaatcaa acaccgcggt tatttgtcgc
     5101 aaagaaaaaa cgagggcgaa actgccgata aagagcttgg cgctttgctc aaaggcgtag
     5161 ccgacaatgc ccatgcctta cagacaggcg atttccgcac accggccgaa ttggctttaa
     5221 ataaatttga gaaagaaagc ggccatatcc gcaatcagcg cggcgattat tcgcatacgt
     5281 tcagccgcaa agatttacag gcggagctga ttttactgtt tgaaaaacaa aaagaatttg
     5341 gcaatccgca tgtttcaggc ggccttaaag aaggtattga aaccctactg atgacgcaac
     5401 gccctgccct gtccggcgat gccgttcaaa aaatgttggg gcattgcacc ttcgaaccgg
     5461 cagagccgaa agccgctaaa aacacctaca cagccgaacg tttcatctgg ctgaccaagc
     5521 tgaacaacct gcgtatttta gagcaaggca gcgagcggcc attgaccgat accgaacgcg
     5581 ccacgcttat ggacgagcca tacagaaaat ccaaactgac ttacgcacaa gcccgtaagc
     5641 tgctgggttt agaagatacc gcctttttca aaggcttgcg ctatggtaaa gacaatgccg
     5701 aagcctcaac attgatggaa atgaaggcct accatgccat cagccgtgca ctggaaaaag
     5761 aaggattgaa agacaaaaaa tccccattaa acctttctcc cgaattacaa gacgaaatcg
     5821 gcacggcatt ctccctgttc aaaaccgatg aagacattac aggccgtctg aaagaccgta
     5881 tacagcccga aatcttagaa gcgctgttga aacacatcag cttcgataag ttcgtccaaa
     5941 tttccttgaa agcattgcgc cgaattgtgc ctctaatgga acaaggcaaa cgttacgatg
     6001 aagcctgcgc cgaaatctac ggagaccatt acggcaagaa gaatacggaa gaaaagattt
     6061 atctgccgcc gattcccgcc gacgaaatcc gcaaccccgt cgtcttgcgc gccttatctc
     6121 aagcacgtaa ggtcattaac ggcgtggtac gccgttacgg ctccccagct cgtatccata
     6181 ttgaaactgc aagggaagta ggtaaatcgt ttaaagaccg caaagaaatt gagaaacgcc
     6241 aagaagaaaa ccgcaaagac agggaaaaag ccgccgccaa attccgagag tatttcccca
     6301 attttgtcgg agaacccaaa tccaaagata ttctgaaact gcgcctgtac gagcaacaac
     6361 acggcaaatg cctgtattcg ggcaaagaaa tcaacttagg ccgtctgaac gaaaaaggct
     6421 atgtcgaaat cgaccatgcc ctgccgttct cgcgcacatg ggacgacagt ttcaacaata
     6481 aagtactggt attgggcagc gaaaaccaaa acaaaggcaa tcaaacccct tacgaatact
     6541 tcaacggcaa agacaacagc cgcgaatggc aggaatttaa agcgcgtgtc gaaaccagcc
     6601 gtttcccgcg cagtaaaaaa caacggattc tgctgcaaaa attcgatgaa gacggcttta
     6661 aagaacgcaa tctgaacgac acgcgctacg tcaaccgttt cctgtgtcaa tttgttgccg
     6721 accgtatgcg gctgacaggt aaaggcaaga aacgtgtctt tgcatccaac ggacaaatta
     6781 ccaatctgtt gcgcggcttt tggggattgc gcaaagtgcg tgcggaaaac gaccgccatc
     6841 acgccttgga cgccgtcgtc gttgcctgct cgaccgttgc catgcagcag aaaattaccc
     6901 gttttgtacg ctataaagag atgaacgcgt ttgacggtaa aaccatagac aaagaaacag
     6961 gagaagtgct gcatcaaaaa acacacttcc cacaaccttg ggaatttttc gcacaagaag
     7021 tcatgattcg cgtcttcggc aaaccggacg gcaaacccga attcgaagaa gccgataccc
     7081 cagaaaaact gcgcacgttg cttgccgaaa aattatcatc tcgccccgaa gccgtacacg
     7141 aatacgttac gccactgttt gtttcacgcg cgcccaatcg gaagatgagc gggcaagggc
     7201 atatggagac cgtcaaatcc gccaaacgac tggacgaagg cgtcagcgtg ttgcgcgtac
     7261 cgttaacaca gttaaaactg aaagacttgg aaaaaatggt caatcgggag cgcgaaccta
     7321 agctatacga agcactgaaa gcacggctgg aagcacataa agacgatcct gccaaagcct
     7381 ttgccgagcc gttttacaaa tacgataaag caggcaaccg cacccaacag gtaaaagccg
     7441 tacgcgtaga gcaagtacag aaaaccggcg tatgggtgcg caaccataac ggtattgccg
     7501 acaacgcaac catggtgcgc gtagatgtgt ttgagaaagg cgacaagtat tatctggtac
     7561 cgatttacag ttggcaggta gcgaaaggga ttttgccgga tagggctgtt gtacaaggaa
     7621 aagatgaaga agattggcaa cttattgatg atagtttcaa ctttaaattc tcattacacc
     7681 ctaatgattt agtcgaggtt ataacaaaaa aagctagaat gtttggttac tttgccagct
     7741 gccatcgagg cacaggtaat atcaatatac gcattcatga tcttgatcat aaaattggca
     7801 aaaatggaat actggaaggt atcggcgtca aaaccgccct ttcattccaa aaataccaaa
     7861 ttgacgaact gggcaaagaa atcagaccat gccgtctgaa aaaacgcccg cctgtccgtt
     7921 aaattaatct atccctgttt caggcggcct gaagcaggga ttttttatat aaaaataagg
     7981 ggaaaaacat gacttggcgc agcctgctta ttcaaaacgg cggcaaacta tccttgcaac
     8041 gccggcaact gctgattcaa caaaacggcg agtcccacac cgtaccatta gaggacatcg
     8101 ccgttatcat catcgaaaat cgcgaaaccc tgattaccgc cccgctccta tcagccttag
     8161 ccaagcatgg tgcaaccctg ctgacctgcg acgagcagtt cctgccctgc ggacaatggc
     8221 tgccttatgc ccaatatcac cgccaactta aaatcctcaa actgcaactg aacataagcg
     8281 aacccttaaa aaagcaactc tggcaacata tcgtccgcca aaaaatcctg aaccaagcat
     8341 ttgtcgcaga cgaaacaggc aatgatcttg cagccaaacg gctgcgcact ttggcatctg
     8401 aagtccgttc aggcgataca ggaaaccgtg aagcccaagc tgccgccctc tactttcaag
     8461 ccctctttgg cgaaaaattt acccgtaatg acaacaacgc cgtcaatgcc gcattgaact
     8521 acacctacgc cgtactccgt gccgcagttg cacgcgccct gaccttatac ggttggttac
     8581 ctgcattagg cttgtttcat agaagtgaac tcaatccatt taatctcgcc gatgacttta
     8641 tcgaaccctt acgcccattg gccgacctga ccgtgataca tttgtacgaa caaggccgtc
     8701 tgaaaactga actgacgctc ggcatcaaac aacacctcat caaaatcctc tactatcaaa
     8761 ccagcatcga acggcaacat ttcagcacct tggctgccat cgacaaaatg atttcgtcat
     8821 ttcaagccgg cgtaaccgat aaaaacgcca aacaactgaa actgcctgaa atcctaccat
     8881 tgaaagaata ccaatatgag tgaggccaaa tttatgagga ttatcgtctt cttcgaccta
     8941 ccggttatca cggcggcaaa gcgcaaagcc gccaatcaat tccgccagtt tttattaaaa
     9001 gacggatacc aaatgctgca actttccgta tacagccgta tcgtcaaagg ccgcgattcg
     9061 ttgcaaaaac accacaacag actgtgtgca aaccttccgc aagaaggctc aatccgctgt
     9121 ttggagataa cagaaaagca atatgccgcc atgaaactgc tgttgggcga gctgaaaacc
     9181 caagaaaaaa aggtcaattc agaccaatta ttgttatttt aagcccattt tttcataaca
     9241 aataaaacgg gaaaccctta tgaaataagg atttcccgtc gaagtattgt agcactgcga
     9301 aatgagaaag ggagctacaa cactatcgag ggtacattcc ctatcagtgt tattgtagca
     9361 ctgcgaaatg agaaagggag ctacaacctt tttgcatctt caataacctc atttacaatt
     9421 gtagcactgc gaaatgagaa agggagctac aacggccgga aagggtgggc gataaatgga
     9481 tttattgtag cactgcgaaa tgagaaaggg agctacaacc acctttaaat ttgcctgaca
     9541 ctttgaccta ttgtagcact gcgaaatgag aaagggagct acaacccatg aaaacctcat
     9601 gcatcgatct atgaaattgt agcactgcga aatgagaaag ggagctacaa cggaggcgac
     9661 cagcgtacca agtacgcagg cattgtagca ctgcgaaatg agaaagggag ctacaacatt
     9721 cgggtcgcaa cagaaacctt taccaaaatt gtagcactgc gaaatgagaa agggagctac
     9781 aacacaaagg cttataactt cttcaatgaa gagattgtag cactgcgaaa tgagaaaggg
     9841 agctacaaca aatcatcaag tgctttcata gacaatcgaa ttgtagcact gcgaaatgag
     9901 aaagggagct acaaccattt tttacggcat tttatataca tcattattgt agcactgcga
     9961 aatgagaaag ggagctacaa cgtaaagtta gtgcggttgt atcttcatca gattgtagca
    10021 ctgcgaaatg agaaagggag ctacaaccac atttgattgg aacggcggag attgtgtatt
    10081 gtagcactgc gaaatgagaa agggagctac aaccaacgat ttggcggatg tatttgaggc
    10141 tgaattgtag cactgcgaaa tgagaaaggg agctacaacg atgggaactt agttcttaaa
    10201 tctttctcaa ttgtagcact gcgaaatgag aaagggagct acaactgggc gtaatgcctg
    10261 ttgtcgctgg tagcaattgt agcactgcga aatgagaaag ggagctacaa ctcgtgcagg
    10321 attcggtttt ccacctccca aattgtagca ctgcgaaatg agaaagggag ctacaaccga
    10381 actgcaagaa ctccattcac gacccctatt gtagcactgc gaaatgagaa agggagctac
    10441 aacaaaccag ccgatgccgc ctttaaaagt gatattgtag cactgcgaaa tgagaatggg
    10501 agctacaaca ctcaaattca gtctgtcaac ccgttccgca ttgtagcact gcgaaatgag
    10561 aatgggcgct acaaccagcc gttgcgataa gcgaacgtgc gttaaagtca ttgagacaca
    10621 gtgcaattat ggcaagaaag gcgacagata ttgtgtcaca gatttgaaat aagaaaaaac
    10681 cgcccactca cagagtaggc ggaaaacttg cgtttttttg attgtggtcg ggttagctac
    10741 tgcattgact taacagcaaa gcaaccgcta aaatgaactt gtggttcact tcaacggaca
    10801 gcagcaactt tttcccacgc ataaggaatt taagcatttt agcttctcct agtgaagtta
    10861 atcacacagg cgagagccga acaaaaatcc ccgttgcagc ggggattttt gctgttccct
    10921 gtttgatgat taaactaaaa tcttgcaaaa tgagaccttt gcaaaattcc ccaaaatccc
    10981 ctaaattccc accaagacat ttaggggatt tctcatgagc accttctttc aacaaaccgc
    11041 ccaagccatg attgccaaac acatcgaccg tttcccgcta ttgaagttgg accaggtgat
    11101 tgattgacag ccgatcgagc agtacctgaa ccgtcaaaaa aaccgttacc ttagagacca
    11161 ccgcggccgt cccgcctatc c
//
LOCUS       DAWFAN010000059        11067 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWFAN010000059 DAWFAN010000000
VERSION     DAWFAN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.059"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(1..413)
                     /locus_tag="WGO65_001067"
                     /pseudo
     CDS             complement(1..413)
                     /locus_tag="WGO65_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(646..2703)
                     /gene="ppk1"
                     /locus_tag="WGO65_001068"
     CDS             complement(646..2703)
                     /gene="ppk1"
                     /locus_tag="WGO65_001068"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ0327369.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2872..3315)
                     /locus_tag="WGO65_001069"
     CDS             complement(2872..3315)
                     /locus_tag="WGO65_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ0327370.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAINAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(3322..3837)
                     /locus_tag="WGO65_001070"
     CDS             complement(3322..3837)
                     /locus_tag="WGO65_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0327371.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            4107..6737
                     /locus_tag="WGO65_001071"
     CDS             4107..6737
                     /locus_tag="WGO65_001071"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ0327372.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVIAVGDNAFDANQWQEWYGDKENGV
                     LVNSGDLDGMNFQTAFDAIAAKLQSQDAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVSVPADQLPVVLPENVVPDGMGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            6972..7784
                     /locus_tag="WGO65_001072"
     CDS             6972..7784
                     /locus_tag="WGO65_001072"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEZ0327373.1"
                     /translation="MASKPFLKWAGGKHKLVPFIEHNLPTPTRKRLIEPFCGSAALSL
                     ALDFEQYLLNDINADLIGLFRILKEEKSGFIDYARSFFISENNSDSRFYELREQFNFS
                     KNLHERSALFIYLNRHAFNGLCRYNSKGVFNVPFGRYKSPYFPQQEMEGFIQKSDRVE
                     LMCGDFQMILSQTDNTDTVYCDPPYAPLSPTASFTTYAKEGFNLDNQIRLAQSAQQIA
                     PDSQGVLISNHDTEFTRDIYSFAILKTIEVQRNIAAKGSSRKKVGELLAIYD"
     gene            7777..8586
                     /locus_tag="WGO65_001073"
     CDS             7777..8586
                     /locus_tag="WGO65_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0327374.1"
                     /translation="MIDFDKPAEEDAIYQSRLKKIVSDDLVLYNENSLNVMRKILEKY
                     PNGCFDMIFADPPYFLSNDGFSCQNGQMVSVNKGNWDKSKGMAADLEFYEEWLRLCYA
                     LLKPNGTIWVCGTFHNIYLIGYLMQTIGYHILNNITWEKPNPPPNLSCRFFTHSTETI
                     LWAKKNKKAKHTFHYEMMKAQNDGKQMKCVWTFPPPNKAEKTFGKHPTQKPLPLLERC
                     ILSASNIGDLIFDPFMGSGTTGVAALKHGRKFCGCELEEDFFELAKKRLEK"
     gene            8583..9137
                     /locus_tag="WGO65_001074"
     CDS             8583..9137
                     /locus_tag="WGO65_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327375.1"
                     /translation="MIIGGIGGARTQTGLRFEERTDLRTLFEEIPGYDLRKTYDNAGY
                     EVWFNGELKAYCFKKYEFYRFLKRPGYSINWKDHLSKRLLPDNGLFIIIRDTLFIIEI
                     KFQQTPGSVDEKLQTCDFKRKQYTKLVHSLGWRVEYVYVLNDWFTKPEYKDVLDYIIS
                     VNCHYQFNTIPLRWFGLPDGETNE"
     gene            9789..10043
                     /locus_tag="WGO65_001075"
     CDS             9789..10043
                     /locus_tag="WGO65_001075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327376.1"
                     /translation="MPRGRGCFGFHARLRRALLSGYAVCTAAVSAWFDAVGHLCGYAL
                     IFSSAVLPVLAGIFSSASIVNLLILPFAKSATQWLRYPCF"
     gene            complement(10012..10323)
                     /locus_tag="WGO65_001076"
     CDS             complement(10012..10323)
                     /locus_tag="WGO65_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ0327377.1"
                     /translation="MKYKSEIFAAIHETMQGLHDIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(10320..10688)
                     /locus_tag="WGO65_001077"
     CDS             complement(10320..10688)
                     /locus_tag="WGO65_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0327378.1"
                     /translation="MRIFKNQWIVKFAKKHKISDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLIFFRYGERAFFMTAFAKNDRENITDKELAELKKAAAIILA
                     MTETDIEQAKSNGTFTEIQP"
BASE COUNT         2671 a         2812 c         2862 g         2722 t
ORIGIN      
        1 gtcgcagctg ctcagactat cggtataaac aatactgtcc ggcatgattt tctttttgat
       61 gacagggagt aacgtttcag acttggcatt atccaccaca acggtataga cccgtccgtt
      121 gcgtttcaga atgccgaaga caaccacttt tcctgccgcc cgcgaccacg tctgccttta
      181 cgccgtccgc cgaaatcgct ttcgtccggc tcgacagggc cctcaaaaac ctcatcggca
      241 gccaaggcca aatgatggtt gataaccgtg cggattttac ggtagaacag tgctgccgaa
      301 ttgggatgga tacccaaaat atcggcggca gaacgggcgg taacttcgag tacaaaaaaa
      361 cggagcagtt ctttctgtac ttttttcttt catttgcagt gcgttatctt catatttcga
      421 gggtaacata tctgctaatc tagtacagcc cctaaaattt aatccactat aatgccgtct
      481 ggaatgcggc ggattggggt tgggagcgta taggggaagt gcttatgcgg aaatggcgaa
      541 ggtgtgtttt tgactccggt ccgccctgtt tagattgctg taaaaccatg ccgtctgaaa
      601 cggaaaggtt tcagacggca tggtttttga ttggggcggt gcgggtcagc ctccgagcag
      661 tgtccacaaa tcgttttgca ggtcggcttc ggattcgccc tcggcaggtg cggcgcggat
      721 atagccgccg tcgggctgca tcagccacgc gtgcgtattg tctgccaatg ccatttccaa
      781 accttcgcgg atgacgcgtt ttttgagttc gggcgcggta atcggcgcgg cggtttcgat
      841 gcggcggaag aagttgcgcc ccatccaatc cgcgctggag ataaaggtat cgtccgcgcc
      901 gttgttgtgg aagcaataca cgcgcgcgtg ttcgagctgc ctgccgatga tggagcggac
      961 gcggatgttt tcggacaagc cttttacacc cgggcgcaag gtgcacatac cgcgcacaat
     1021 caaatcgatt tgtacgcctg ccgcgcttgc ccgatacagg gcttcgatga cggtcggttc
     1081 gatgagcgaa ttcatcttgg cggtaatccg cgccggtttg ccggcttttg cgtgttcggt
     1141 ttcgcgtgcg atgcggtcga taaccatttt gtgcagggta aacgggcttt ggtagagttt
     1201 gttcagccgc ccgggtttgc ccaagcctgt gatttccata aacaatgtgt tcacatcggc
     1261 ggtgatttgt tcgtcggcgg taatgatgcc gaagtcggtg tagatgcgcg atgtgccttg
     1321 gtggtagtta cccgtgccga ggtgggcgta acgcttgagt acgccgtctt cgcggcggat
     1381 gaccagtgcc atcttggcgt ggactttgta gccgaacacg ccgtacacga cgtgcgcgcc
     1441 cgcctcttcg agctgcttcg cccagttgac gttgttggct tcgtcaaaac gcgccatcag
     1501 ttcgacgacg acggttactt gtttgccggc gagtgccgcc ttcatcaggg cgcggacgag
     1561 ttcggaacgc gtgccggtgc ggtaaatcgt cattttgacg gcaagcacgt cggggtcggc
     1621 ggcagcttcg cgcatcattt cgacaacggg gtcgaacgat tggtaggggt ggtgcagcag
     1681 gatgggcgat tggcgcacca aatcgaatat ggacgcggtt ttgcccaagg ctttcagtct
     1741 gcccggcgtg tgtgtgggaa atttcaaatc ggggcggttg actaggtcgg ggacggcgtt
     1801 gaggcgcacg aggttgaccg ggcctttgac ctgatagagt tcggcggcgg tcagtttgaa
     1861 ttgcgcgagc agaaagtcgc ggatgtaggc gggacaggtg tcggcgactt cgagccgcac
     1921 gccgtcgccg tattcgcggt cgtgcagttc gttttggatg gcggcgcgga ggttttgcag
     1981 gtcttcttcg tcaacggtca agtcgctgtc gcgcgtcagg cggaactggt ggcagccttt
     2041 gacgttcatg cccgggaaga gtttgccgac gtgggcgtgc aggatggagg agaggaagac
     2101 gaagccgtgt ccgccgccac acagttcgga cggcagggga acaacgcgcg gcaggatgcg
     2161 cggtgcctgc acaatcgcca tccccgaagg cctgccgaac gcgtccgtgc cgtcgagttc
     2221 gacggcgaag ttgagcgatt tgttcagcgg tcgcgggaag gggtgggacg ggtcgagtcc
     2281 gatgggggtc aggatcggca gcagttcgcg gtcgaaatag tcttcgatcc attttttctg
     2341 cgcgcccgtc cagttgcggc ggcggtaaaa atggatgctt tcccgcgcca actcgggctg
     2401 aaggacgttg ttgaacaggt cgtactggtg ccgtatcagg gagcgcgccg cttcggtaac
     2461 gtcggcgatg gtttcagacg gcattttgcc gttgtccagc ctgcgccggg ggtgcagctt
     2521 gttttcacgc ttgagccacg ccatgcggac ttcgaaaaac tcgtcaagat tggacgacac
     2581 gatgcacagg aagcgcaggc gttccaaaag ggggacgttt ttgtcttccg cctgtgccaa
     2641 cacgcggcgg ttgaatgcca gcaggcttag ttcgcggcag aggatgcggt tttgttcggg
     2701 cataaggttc tcccaaagat ggttgttgtt cggtcggagc gggcaggcaa tgccgtctga
     2761 aggcttggcg gcatctgcgc cggtttcaga cggcatgacg tggcggacag cctgtttcag
     2821 gcatcggggc aatgtgaaaa cagataccgg cgcgtgcggt atccgtctgt ttcaaatcac
     2881 ttccaacaca aaataatgac gcagtttttc gtacaccgca tcgctgacgt tgatgcagcc
     2941 gttggtcata atcctgtcgg acacggacgg ggaggcgatg cgttcgttcc gcctttccga
     3001 cggtatctga ttccaaacgc ggtgcagggc gaaaagaaaa tcaccctcct gcttgaaacc
     3061 gatgacttcg ccgccgtatc caggcttgtc ggtactgttc agcgtcagcc cgaacgtccc
     3121 cttgggcgtg gcaacaccga tgaggacggg gtggcactga cggtcgtcag cgaaacagag
     3181 ttccgctttg gatgtatcga cgatgacttt tttcttttgg atataggcat tgatcgcatc
     3241 cggttgcccc tgtgcatata cgggggcaac cattgacagg ccgcataata tccctaaaaa
     3301 caggcggaac ggcatgtcgg attattggcg gatacgtttc ggttttgccg gcacgatttc
     3361 gcggatgatg acttgcggct cggctttggg tgcgggcggc ggacaggcgg catctttcgg
     3421 gaagacgggg ttccagtaga agctgcgggc aaatttgtct ttatcgaaaa tcactttgta
     3481 ttggcaggta gtaacatctt ctacgccgga agtgttttca gggtcgatac ctacgcccgg
     3541 ggtatggaag tggaacaggt aatcccattc gcgcacgccg tacatacctt cgtcgtaatg
     3601 cgggcggccc aggattttgt agatgtcgtc tttggtcagg ccggggcgca tctgatccag
     3661 ttcgtcataa gtcgggaatg tgccgcgctt gttgtcgagc gttacggaat agggtttcgg
     3721 gaaaaccgga ttgtcggtcg tgccgtcggc tttgacgttg cttttggttg cgcaggcaga
     3781 cagaacgccc gccgccaata ctgccaagcc cggtttgacg atttgtttga ttttcatatg
     3841 cagaatcctc ttttacccga tgcccgtctg ccttttgttc ggaagagccg catcaggaga
     3901 gttttaaaca tttgattttg gtttgtaata ttagcataaa aaacgtgcgt atcaataaac
     3961 gcggtgtatt tgtacggcat acggaataat gcgcgtgcga atttacgcat cctgccggca
     4021 atctgccgat tcgccgcttc cggcaacctg ttataattga tgccgacttt atttttccaa
     4081 cgtttttaag cgaaaacaga atgactatgc aagaacaata tcaacccgcc gccatcgagc
     4141 ctgcggcgca gaaaaaatgg gacgacgccc gtattttcaa cgtctccgaa gacgcttcca
     4201 aacccaaata ttattgcctc tccatgttcc cctatcccag cggcaagctg cacatggggc
     4261 atgtgcgcaa ctacaccatc ggcgatgtat tgagccgctt caaactttta aacggcttca
     4321 acgtcatgca gcctatgggt tgggacgcgt tcggcatgcc ggcggaaaac gcggcgatga
     4381 aaaacaacgt cgcccctgcc gcttggacgt acgataacat cgaatacatg aaaacccagc
     4441 tcaaaagcct gggttttgcg attgactggg cgcgcgaaac cgcaacctgc aaacccgaat
     4501 actaccgctg ggaacaatgg ctgtttacca agctgtttga aaaaggcatc gtttatcgca
     4561 aaaacggcac ggtaaactgg gacccggtcg accaaaccgt ccttgccaac gagcaagtta
     4621 tcgacggacg cggttggcgt tcgggcgcgt tgatcgaaaa acgcgaaatc ccgatgtatt
     4681 acttcaaaat caccgattac gccgaagagc tgctcaacga cttggacaag ctggaacact
     4741 ggcctgaaca agtcaaaacc atgcagcgca actggatcgg caaatctcgc ggcatgaccg
     4801 tgcgcttcgc cgtttcagac gacagcaaac aaggcttgga aggcgattac gcgaaattcc
     4861 tgcaagttta taccacccgc cccgacacgc tgatgggcgc gacttatgtt gccgttgccg
     4921 ccgagcatcc gctggcaacc gcagccgccg ccgacaaacc cgaattgcag gcatttatcg
     4981 ccgaatgcaa agccggttcg gttgccgaag ccgatatggc gacgatggag aaaaaaggcg
     5041 tgccgaccgg tcgctacgtc gtcaacccgc tcaacggcga taagctggaa gtgtggattg
     5101 ccaactatgt attgtggggc tacggcgacg gcgcggtaat ggcggttccg gcgcacgacg
     5161 aacgcgattt cgagttcgcc gccaaataca gcctgccgaa aaaacaggtc attgccgtcg
     5221 gcgacaacgc atttgacgca aaccaatggc aagagtggta cggcgacaaa gaaaacggcg
     5281 tattggtcaa tagcggcgac ttggacggta tgaattttca gacggcattc gacgccatcg
     5341 ccgccaagct gcaaagccaa gacgcgggcg aaccgaaaac ccaataccgc ctgcgcgact
     5401 ggggtatttc ccgtcaacgc tactggggct gcccgattcc catcgtccat tgcgaaaaat
     5461 gcggcgacgt atccgtcccc gccgaccaac tgcccgtcgt cctgcctgaa aacgtcgtac
     5521 ccgacggcat gggttcgccg ctggcaaaaa tgcccgagtt ttacgaaact acctgcccat
     5581 gctgcggcgg cgcggcgaaa cgcgaaaccg acaccatgga caccttcatg gaatcgagct
     5641 ggtatttctt ccgctatatg tcgcccaagt tttcagacgg catggtatcg gctgaagccg
     5701 caaaatactg gggcgcggtc gaccaataca tcggcggcat cgaacacgcg attttgcacc
     5761 tcttgtacgc gcgtttcttc accaaactga tgcgcgacga aggtctagtc aatgttgacg
     5821 aaccgtttga acgcctgctg acgcaaggca tggtcgtttg cgaaacctac taccgcgaaa
     5881 acgacaaagg cggcaaagat tggatcaacc ccgccgatgt cgagctgacg ttcgacgaca
     5941 aaggccgccc cgtttccgcc gtcctcaaag ccgacggact gcccgtcgtc atcagcggca
     6001 cggaaaaaat gtccaagtcc aaaaacaacg gcgtcgatcc gcaagagctg attaacgcct
     6061 acggcgcgga caccgcccgc ctgttcatga tgttcgccgc accgcccgaa cagtccctcg
     6121 aatggagcga cagcggcgtc gaaggcgcgc accgcttcct gcgtcgcttg tggcgtaccg
     6181 tttacgaata cctgaaacaa ggcgaagcag tcaaagcatt tgcaggcagc caagacggtt
     6241 tgtctaaaga actcaaagac ctgcgccaca aactgcacgc gaccactgcc aaagtcagcg
     6301 acgactacgg ccgccgccag cagttcaaca ccgccatcgc cgccgtgatg gaattgctca
     6361 accaatacga caaaaccgac accggcggcg aacaaggacg cgccgtcgcc caagaagtat
     6421 tggaaaccgc cgtacgcctg ttgtggccca tcgtgccgca catctgcgaa accctgtgga
     6481 gcgaattgaa cggcgcgaaa ctgtgggaag caggctggcc gacagtcgac gaagccgcct
     6541 tggtcaaatc cgaaatcgaa gtcatggttc aagtcaacgg caaactgcgc ggaaaaatca
     6601 ccgtcgccgc cgacgcatcc aaagccgacc tcgaagccgc cgcactcgcc accgagggcg
     6661 cagtgaaatt catggaaggc aagcccgcca agaaaatcat cgtcgtgccg ggcagactgg
     6721 tgaacatcgt cgtctaaccg cttttaaggt ttatccacac ggataaaggc cgtctgaaac
     6781 ttggaaacag ggtttcagac ggccttttta aggcagatgg agttgataag tccaataaca
     6841 tattggaatg gaaaggttct attggattta gactcactct acacacgccc ccaaatataa
     6901 atccattgta ttcctcacga tgttttggca caattccaag ccaatcaacc acgcttgtac
     6961 tcggcatcct aatggcttca aaaccttttc taaaatgggc aggcggcaaa cataaacttg
     7021 tcccctttat cgaacacaat ctacctactc ccacacgaaa acgcctgatt gaaccctttt
     7081 gcggttctgc cgctttgtct cttgcattgg atttcgaaca atatctgctc aatgatatta
     7141 acgctgactt aatcggctta ttccgtatcc taaaagaaga aaaatccgga ttcatcgact
     7201 acgcccgctc tttctttatt tccgaaaaca atagcgattc aagattctat gaattgcggg
     7261 aacaattcaa cttcagcaaa aatctgcacg aacgctcggc actatttatt tatttgaacc
     7321 gtcatgcttt taacggttta tgccgttaca acagcaaagg ggtattcaat gtgccgttcg
     7381 gacgctacaa atcgccgtac tttccccaac aagaaatgga aggtttcatt cagaaatctg
     7441 acagagttga attaatgtgc ggagattttc aaatgattct atctcagaca gacaataccg
     7501 atacggttta ttgcgatccc ccttacgcgc ctttaagccc aaccgcttca tttaccactt
     7561 atgccaaaga aggtttcaat ttagacaatc aaatccgtct tgcccagtca gcccaacaaa
     7621 ttgcgcccga ttcccaaggc gttttgattt caaatcatga tactgaattc acaagggata
     7681 tttattcttt tgctattctg aaaacaattg aagtacaacg caatattgcc gcaaaaggca
     7741 gcagccgtaa aaaagtcgga gaattattgg caatttatga ttgattttga caaaccggct
     7801 gaagaagacg ccatttatca aagtcgtttg aaaaaaatcg tttcagacga ccttgtcctc
     7861 tacaacgaaa attcccttaa cgtcatgcgg aagatattgg aaaaatatcc aaacggctgt
     7921 tttgatatga ttttcgcaga cccgccttac tttctttcca atgacggctt cagttgtcaa
     7981 aacggacaaa tggtttccgt caataagggc aactgggata aatccaaagg aatggcggca
     8041 gatttggaat tctacgaaga atggctccga ctgtgttacg ccttattaaa accaaacggc
     8101 acaatttggg tttgcggcac attccataat atttatttaa tcggctacct gatgcaaact
     8161 atcggatacc atattttgaa caatattact tgggaaaaac ccaatcctcc ccctaatttg
     8221 tcctgccgtt tttttaccca ttcgacagaa acaatcttat gggcaaagaa aaataaaaaa
     8281 gccaaacaca cgtttcatta tgaaatgatg aaagcacaaa atgatggcaa acagatgaaa
     8341 tgtgtttgga catttcctcc tccaaacaaa gccgaaaaaa cattcggcaa acatccgaca
     8401 caaaaaccac tccccttgct tgaacgctgc atactctcag cttcaaacat cggagattta
     8461 atttttgacc ctttcatggg cagcggcaca acaggcgttg ccgccttaaa acatggtcgg
     8521 aaattttgtg gttgcgaact ggaagaagat ttttttgaat tagcaaaaaa aaggttagaa
     8581 aaatgattat tggcggaatc ggtggtgcaa ggacacaaac aggactcaga ttcgaagaac
     8641 gtacagactt acgaacgttg tttgaagaaa ttcccgggta cgacttaaga aaaacatatg
     8701 ataatgcggg ttatgaagtt tggttcaatg gagaattgaa ggcttattgt tttaaaaaat
     8761 atgagtttta ccgatttttg aaaagaccgg gatacagtat taattggaaa gaccatctgt
     8821 ctaaaagatt actacccgac aatggcttat ttatcatcat ccgtgatacc ttatttatta
     8881 ttgagatcaa attccaacaa actcccggtt cagtagatga gaaattacaa acttgcgatt
     8941 ttaagagaaa acaatataca aaattggttc actctttagg ttggcgggtt gaatatgtct
     9001 atgttttgaa tgattggttt acaaagccgg aatataaaga tgttttagat tatattatca
     9061 gtgttaactg ccattaccaa tttaacacga ttcccttaag gtggtttgga ctgcctgatg
     9121 gtgaaacaaa tgaataaata ccatatttaa aacacagccg ttcaaggtcg tctgaatatc
     9181 atttcagacg acctttcacc aatccccgcc gttttccatc tttctgctat tgtgataaag
     9241 tagcccgacc ttttgttcaa aatacgcgga ttccgcgaca ctgaatgcag catctgtcaa
     9301 agattatttg aaaacggtcg agttagacaa gtctgccgac aatgtcgata ccacatccaa
     9361 aactatccgc agggtttagg cggtattcca gagtacggtg tcttatgctg atgctgtaaa
     9421 caatgcccgt atagaaaatc taaaacggtt gttgccgacc gttatgccgg tttatgagca
     9481 aagtgtcaga aataaggggc gcgttaatat agtggattaa atttaaatca ggacaaggcg
     9541 acgaagccgc agacagtaca gatagtacgg caaggcgagg caacgctgta ctggtttttg
     9601 ttaatccact ataaaaaacg tcggcgttaa gggaagggga tcgagaattt gagccgttgt
     9661 ttcaaaatgc cgtctgaaat cgtttgagat tcagacggca tttaaacagt tctgcgcccg
     9721 cccgttttgc cttcaagccc gcgccgctta aatccaatcc cccctgcaac gggcgcaaaa
     9781 aaatctccgt gcctcgcggt cggggatgtt tcgggtttca cgcccgtctg cgccgtgcct
     9841 tgctgtcggg ctatgccgtc tgcaccgccg ccgtttcggc ttggtttgat gcggtcgggc
     9901 atttatgcgg ctacgccctg atattcagtt cggcggtctt gcctgttttg gcaggcatat
     9961 tcagcagcgc gtctattgtg aacttgctga ttttgccgtt tgccaagtcg gctacgcaat
    10021 ggcttcgata cccttgtttt tgacgatggt cagcagcttc aacgccgcgc cgctgggctt
    10081 tttaacgccc cgctcccaag ccgaaacgtg attttttccc acgttgagat agatggcgaa
    10141 agcggcttgc gatagtgcct ccttctccct gattgccttg atgtccccgc cgctcaacgg
    10201 tttgatttcg gtcaggcagg acttgtcaaa gccgcgcata gtctttttgt cgattgcgcc
    10261 gatgtcgtgc agcccctgca tggtttcatg tattgctgcg aaaatctcac ttttgtattt
    10321 catggctgta tctccgtaaa agtgccgttt gatttggctt gctctatgtc ggtttctgtc
    10381 attgccaaga tgatggcggc ggctttcttt aattctgcaa gttctttgtc cgtgatgttt
    10441 tcgcggtcgt tcttggcaaa ggcggtcatg aaaaacgccc gttctccata cctgaaaaat
    10501 atcaggctgc gataaccgcc gcttctgcct tgtccttgcc ttgctatgcg ctgcttaata
    10561 acgccaccgc caaggtctgc gtctatcagc ccgttatccg cccgctctac cgcctccagc
    10621 agctcggaat cactgatttt atgtttcttg gcaaatttca ctatccattg gtttttgaat
    10681 atccgcattg tgtagccgtt gattagtgat acttggaatt ataccagaga cagacaacac
    10741 ggcaagctgc cttttttatg ccccgcaatt tctccgttta tgccgcctga aacactggcg
    10801 gctaacctga ttatttttta atcaatcgct tttaatggct caaattgcgt ttttagcgcg
    10861 tttcgctgtc ttcctatgtc ccccatatat aaccaagcgg tttaaccgcc attcctgctc
    10921 aaaataggcg cggttccgtg cgttttggct tttcattccg ctacgctccc actgccaaga
    10981 cggtgaaaaa ccggaaaacc ccaaaagttg ttaccgattc gttaccattt gttaccgcat
    11041 ttgttaccaa ttttttatct ttttctt
//
LOCUS       DAWFAN010000060        10979 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWFAN010000060 DAWFAN010000000
VERSION     DAWFAN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.060"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(14..604)
                     /locus_tag="WGO65_001078"
     CDS             complement(14..604)
                     /locus_tag="WGO65_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0327379.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            complement(665..1246)
                     /locus_tag="WGO65_001079"
     CDS             complement(665..1246)
                     /locus_tag="WGO65_001079"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ0327380.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAELYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            1324..1557
                     /locus_tag="WGO65_001080"
     CDS             1324..1557
                     /locus_tag="WGO65_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327381.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFT
                     ITVRRRNHLMRQQEQAESEQQRAQRQKDSGTKP"
     gene            1730..2458
                     /gene="rpsB"
                     /locus_tag="WGO65_001081"
     CDS             1730..2458
                     /gene="rpsB"
                     /locus_tag="WGO65_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ0327382.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            2587..3441
                     /locus_tag="WGO65_001082"
     CDS             2587..3441
                     /locus_tag="WGO65_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ0327383.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPATVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            3658..4377
                     /locus_tag="WGO65_001083"
     CDS             3658..4377
                     /locus_tag="WGO65_001083"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ0327384.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            4561..5523
                     /gene="mafA"
                     /locus_tag="WGO65_001084"
     CDS             4561..5523
                     /gene="mafA"
                     /locus_tag="WGO65_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0327385.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIQPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            5563..7008
                     /gene="mafB"
                     /locus_tag="WGO65_001085"
     CDS             5563..7008
                     /gene="mafB"
                     /locus_tag="WGO65_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0327386.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     SGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYHIKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDVRGIVQGAANPFLTGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            6974..7357
                     /locus_tag="WGO65_001086"
     CDS             6974..7357
                     /locus_tag="WGO65_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327387.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            7574..8290
                     /locus_tag="WGO65_001087"
                     /pseudo
     CDS             7574..8290
                     /locus_tag="WGO65_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            8295..8654
                     /locus_tag="WGO65_001088"
     CDS             8295..8654
                     /locus_tag="WGO65_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0327388.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            8730..8942
                     /locus_tag="WGO65_001089"
     CDS             8730..8942
                     /locus_tag="WGO65_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327389.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            9039..9884
                     /gene="mafB"
                     /locus_tag="WGO65_001090"
                     /pseudo
     CDS             9039..9884
                     /gene="mafB"
                     /locus_tag="WGO65_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            9894..10100
                     /locus_tag="WGO65_001091"
     CDS             9894..10100
                     /locus_tag="WGO65_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327390.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            10232..10483
                     /locus_tag="WGO65_001092"
     CDS             10232..10483
                     /locus_tag="WGO65_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327391.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            10480..10851
                     /locus_tag="WGO65_001093"
     CDS             10480..10851
                     /locus_tag="WGO65_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0327392.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT         3381 a         2516 c         2392 g         2690 t
ORIGIN      
        1 tgaaaatact gcctcacacc tgcacgccat accctgccaa cctgccggtc aggatttccc
       61 tgtttttgca ccaatcttcc ctcagcatac tgtacacgac cgtatcgcgc acactgccgt
      121 ctttacggag catatgcata cgcagcacgc cgtctttttc cgcacccagc cgttcgatgg
      181 cacgttgcga ggcaaggttc agaatatccg tgcgccatcc cacgcaacgg caagccaaaa
      241 catcaaatgc gtaatccaac agcatgattt tgcaacaggt gtttatccgt gtccgccgtg
      301 ccgatgccgc ataccatgtg aatccaatat ccaaacgcgg aatctgcggt tcaaaatgat
      361 aatacgccgt tgtcccgacc accctgcccg cctcttcatc gacaaccgca aacgccaaac
      421 gcgttgccaa tgctgtcccg atatagtctg ccaccctatc cggatggggc gcggacgtta
      481 ctcccagctt ccaaacttcc ccatcgcaaa ccgcctcgcg caaacccgtt tcatgatgca
      541 catccaacgg ttcgagccgt acgccaccca acgacaagac cggcagtatt atcttttccg
      601 acatcctttt ctcccaatat tccaccttca gacggcattt ccgttcaaaa tgccgtctga
      661 acggctaaaa acacaatatc cccgcctccg acacaaaacc gtccagcggc aggtcgtgcg
      721 cctcgaccga cagtctgtcc accaactggc aggcaaagcc cacgcccacg gtttttgcct
      781 gcaaacggta tttcatcgct gcaagcgtcg catcgtaata gccgcctgcc tgccccaaac
      841 ggtagccttc gcggtctatg ccgacaagcg gaacgagcaa taccgacaaa ccgtgcacgc
      901 ggattttgcg ccctgcaaac tgcggcacgt tcaacctcgc cctgccccgt atgcgctccc
      961 gttccattcc gctttcagga tacggcgtaa accacatccg ccgcgaacgc ggttcgatat
     1021 aaggcaggta gagttccgca ccgcgttttt gcgccgcgcg gacaaagccg tccaaacgca
     1081 attccttgcc catcggccaa tacacgccga ttttccgccc ttttttaata taacgtttga
     1141 gcaggtggtt gatttttacc gttgccgccg cccgcacgtc ccgccccatt tgcgaacgcc
     1201 gcccgcgcaa ttcgcggcgc agggcgcgtt tttcctcatt cctcatttca gacggccttt
     1261 caggattgcg gtagaatgtt gcgattataa cgattttgtt aacattcaaa caggacgcac
     1321 acaatgtggc acatcgtcgc catcggctat ctttttgttg ccgttatgta ttccgccgcg
     1381 cagccgagta ttgcgcgcgc cttgatttat ttggtttttt gggcggtgct gcccaccgtg
     1441 ttcacggttt tcaccattac cgtccgccgc cgcaaccacc tgatgaggca gcaggaacag
     1501 gcggaatccg aacagcagcg cgcacaacgg caaaaagaca gcggcacaaa accctgaatc
     1561 ccttttcaga cggcatctta tccgctataa tccgtcagtt ttccatttcg gaaacacact
     1621 attttttaaa acttatgccc actttcgccg aagggtgctt gacaataggc gtgacctatc
     1681 aagttctatg cgattgaatg tgtgctctta accctttcaa ggaaataaaa tgtctcaaat
     1741 tactatgcgt cagatgattg aagccggtgt tcacttcggc caccaaaccc gtttctggaa
     1801 cccgaaaatg gcacaataca ttttcggtgc gcgcaacaaa atccatatcg taaacttgga
     1861 aaaaaccctg ccgatgttcc aagacgcgca agaagccgta cgccgtttgg ttgccaacaa
     1921 aggtacagta ttgttcgtag gtaccaaacg ccaagcccgc gacatcatcc gcgaagaagc
     1981 gacccgtgcc ggtatgcctt tcgttgatta ccgctggttg ggcggtatgc tgaccaacta
     2041 caaaaccgtt aagcaatcca tcaaacgcct ggaagaaaaa accgcagcct tggaaaatgc
     2101 tgccgaaagc ggtttcagca aaaaagaaat tctggaaatg caacgcgatg ttgaaaaact
     2161 ggaacgttct ttgggcggta tcaaaaacat gaaaggcctg cctgacgcga ttttcgttat
     2221 cgataccggc taccaaaaag gtactctggt tgaagctgaa aaattgggca tccctgttat
     2281 cgccgtagtc gataccaaca acagccccga cggcgtgaaa tacgttatcc ccggcaacga
     2341 cgactccgcc aaagccatcc gcctgtactg ccgcggcatc gctgacgcag ttttggaagg
     2401 caaaaaccaa gcgctgcaag aaaccgtagc cgctgcccaa gaagccgctg ccgagtaatc
     2461 cggcaaaccg aagaggggcg ttatgcccct tttctcaaat atgccgtctg aacgtccgtt
     2521 cgcggcacac gattcccgaa tgcggaaaat cctttccgta tttcccaaaa atctaggaga
     2581 ttcaaaatgg cagaaattac tgcaaaaatg gttgccgatc tgcgcgccgc taccggcctg
     2641 ggcatgatgg aatgcaaaaa agccttggtt gaagccgaag gcaacttcga caaagccgaa
     2701 gaaatcctgc gtatcaaatc cggtgcgaaa gccggtaaac tggccggccg taccgctgcc
     2761 gaaggcgtat tggcttacgc gatcaacggc aatgtcggcg cattggtcga agtaaactgc
     2821 gaaaccgact tcgtcgcgaa agacgccggc ttcgtagaat ttgccaactt cgttgctaaa
     2881 actgctgccg agaaaaaacc ggctaccgtt gaagaactga gcgaactggt tgaagcagaa
     2941 cgcaaagcca tcatcgctaa attgggcgag aacatgtctg tccgtcgctt ccaagtgatc
     3001 gacactgcca accaactggt tgcctacatc cacggcgcat tggcgaccga aggcgtattg
     3061 gttgagtaca aaggctctga agacgtagca cgcaaaatcg gtatgcacat tgttgccgct
     3121 aaaccacaat gcgtaagcga agccgaagta gatgccgaaa ccgttgaaaa agaacgccac
     3181 atctacaccg aacaagccat cgcttccggc aaacctgccg acatcgccgc taaaatggtt
     3241 gaaggccgta tccgcaaatt cttggctgaa atcactctga acggccaagc attcgtgatg
     3301 aaccccgatc aaactgttgc ccaattcgcc aaagaaaacg gcactgaagt ggtcagcttc
     3361 gtccgctaca aagtaggcga tggcatcgaa aaagccgttg tcgactacgc agccgaagta
     3421 gccgcagccg ccaaagtgta aggcacttat gaaaaagaaa gcacctggat tccaaacgaa
     3481 tcagggtgct tttttttgag aaaaccgttt gcggtaccta ttttaagacg accgaatatt
     3541 cagaccgtct taaaacaaaa caataataaa ccgacacacc ctatcattaa tattccgacc
     3601 gttggaaatt cagacggccc aactccgacc gacgacattc aagaaagcaa ggtatccatg
     3661 acacagcaaa tcaaatacaa acgcgtatta ctgaaactct ccggcgaatc cctgatgggt
     3721 tccgatccgt tcggcatcaa tcacgatacc atcgttcaaa ctgtcggcga aattgccgaa
     3781 gtcgttaaaa tgggcgtgca agtcggtatt gttgtcggcg gcggcaatat tttccggggc
     3841 gtatccgccc aagcaggcag catggatcgc gccaccgccg actacatggg catgatggcg
     3901 accgtgatga acgcgttggc actcaaagac gcatttgaaa ctttaggcat caaagcgcgc
     3961 gtacaatccg cactgtctat gcagcaaatc gctgaaacct acgcccgccc caaagccatc
     4021 caatatttgg aagaaggcaa agtcgtgatt tttgccgccg gtaccggtaa cccgttcttc
     4081 acgaccgaca ctgccgccgc attgcgcggt gcggaaatga actgcgacgt gatgctcaaa
     4141 gccaccaacg ttgacggcgt gtacaccgca gaccccaaaa aagactcatc cgccacgcgc
     4201 tacgaaacca ttacttttga cgaagccttg ttgaaaaacc tcaaagtgat ggatgccacc
     4261 gctttcgccc tctgccgcga acgcaagctc aatattgtcg tattcggcat tgctaaagaa
     4321 ggctcgctca aacgtgtagt aaccggtgaa aacgaaggta cgctggttca ctgctgattg
     4381 accatagtgt cggcagatat agtcgcatat gggcttcaga cagccattta ttatatggag
     4441 attatagtgg attaaattta aaccagtacg gcgttgcctc gccttgccgt actggtttaa
     4501 atttaatcca ctatatttac aattttgata caatttgttt ttcatcaaag gagaaaatct
     4561 atgcaagcac ggctgctgat acctattctt ttttcagttt ttattttatc cgcctgcggg
     4621 acactgacag gtattccatc gcatggcgga ggtaaacgct ttgcggtcga acaagaactt
     4681 gtggccgctt ctgccagagc tgccgttaaa gacatggatt tacaggcatt acacggacga
     4741 aaagttgcat tgtacattgc aactatgggc gaccaaggtt caggcagttt gacagggggt
     4801 cgctactcca ttgatgcact gattcgtggc gaatacataa acagccctgc cgtccgtacc
     4861 gattacacct atccacgtta cgaaaccacc gctgaaacaa catcaggcgg tttgacaggt
     4921 ttaaccactt ctttatctac acttaatgcc cctgcactct ctcgcaccca atcagacggt
     4981 agcggaagta aaagcagtct gggcttaaat attggcggga tgggggatta tcgaaatgaa
     5041 accttgacga ctaacccgcg cgacactgcc tttctttccc acttggtaca gaccgtattt
     5101 ttcctgcgcg gcatagacgt tgtttctcct gccaatgccg atacggatgt gtttattaac
     5161 atcgacgtat tcggaacgat acgcaacaga accgaaatgc acctatacaa tgccgaaaca
     5221 ctgaaagccc aaacaaaact ggaatatttc gcagtagaca gaaccaataa aaaattgctc
     5281 atcaaaccaa aaaccaatgc gtttgaagct gcctataaag aaaattacgc attgtggatg
     5341 ggaccgtata aagtaagcaa aggaattaaa ccgacagaag gattaatggt cgatttctcc
     5401 gatatccaac catacggcaa tcatacgggt aactccgccc catccgtaga gactgataac
     5461 agtcatgagg ggtatggata cagcgatgaa gtagtgcgac aacatagaca agggcaacct
     5521 tgattcacac gaccataacc gcttgctacc aaggaaaaca aaatgaattt gcctattcaa
     5581 aaattcatga tgctgtttgc agcagcaata tcgttgctgc aaatccccat tagtcatgcg
     5641 aacggtttgg atgcccgttt gcgcgatgat atgcaggcaa aacactacga accgagtggc
     5701 aaataccatc tgttcggcga tggtcgcggc agtgttaaaa atcgggttta cgccgtccaa
     5761 acatttgatg caactgcggt cggccccata ctgcctatta cacacgaacg gacaggattt
     5821 gaaggtgtta tcggttatga aacccatttt tcagggcacg gacatgaagt acacagtccg
     5881 ttcgatcatc atgattcaaa aagcacttct gatttcagcg gcggtgtaga cggcggtttt
     5941 actgtttacc aacttcatcg aacagggtcg gaaattcatc cggaggatgg atatgacgga
     6001 ccgcaaggca gcgattatcc gccccccgga ggagcaaggg atatatacag ttaccatatc
     6061 aaaggaactt caacaaaaac aaagactaat attgtccctc aagccccatt ttcagaccgt
     6121 tggctaaaag aaaatgccgg tgccgcctct ggttttttca gccgtgcgga tgaagcagga
     6181 aaactgatat gggaaagcga ccccaataaa aattggtggg ctaaccgtat ggatgatgtt
     6241 cgcggcatcg tccaaggtgc agccaatcct tttctaacgg gttttcaggg attgggagtt
     6301 ggggcaatta cagacagtgc ggtaaacccg gtaacctatg cggcagcacg gaaaacttta
     6361 cagggtattc acaatttagg aaatttaagt ccggaagcac aacttgcggc cgcaaccgca
     6421 ttacaagaca gtgcttttgc ggtaaaagac agtatcaact ctgccagaca atgggctgat
     6481 gcccatccga acattacagc tactgcccaa actgcccttt ccgtagcaga ggctgcaggt
     6541 acggtttgga gaggtaaaaa agtagaactt aacccgacca aatgggattg ggttaaaaat
     6601 aacggctata aaaaacctgc tgttcgcctt atgcagactg tagacgggga gatggctggg
     6661 ggaaataagc caccgaaacc aagtacgcag caacactcta cacactctga taacaatata
     6721 ggcttacctg ccccatatgt taaacctgat acatctattt ctccgacagg aacaatttat
     6781 gtagacagca aaggaaatgc aataccaaca ccaataggcg gttatataac aggttcgcct
     6841 gatgctcgtt ttatccaagt ccgagatagt aatggaaact tgactggagc cagaatagat
     6901 gatgctcatc atgcaaatac ccatcaagac ccacgcgcac ttacaggaca cgcacatgta
     6961 ccaggagtta caaatgatga cggaacccca tggttaccca tcaaataatt tacctgttaa
     7021 aatcataaat gatattattt atgcaaccca gttagttgaa gatttggttt taggaaaaat
     7081 aaaaattgtt gattttttaa aatcatataa caatttttat tattggcttg gttttgatga
     7141 gttacctcaa tctgagaaaa taaaatttct aaactatctc aatatattaa gtattcataa
     7201 ggaaatacaa gataaaactg tgaatagggt ttataccgat tgttttgata ttgataaatt
     7261 acattcgctt ggtagaatta caactaatga atgtattaat ggtataaaaa aaatctatca
     7321 aaaaaacaaa ttagaatttg acaatatact caaataacta gcctatgttt tgtaaaataa
     7381 aaatattctt gctgagtgta gagccagaga taaaaagatt tgatagtagt ttgcagttgt
     7441 agaattatat aaacaagaaa atagtcagca gccgtaggtc agattctcga atccgacatt
     7501 ttccaacagc ggcatttcgg aaacgataga tgcgtcaaat atttttgtcg gatacaaata
     7561 tccgacctac atctctgcgc agcagactct acaaggtatt aatgatttag gaaatttaag
     7621 tccggaagca caacttgccg ccgtgagcct attacaggac agtgcttttg cggtaaaaga
     7681 cagtatcaac tctgccagac aatgggctga tgcccatccg aatataactg caacagccca
     7741 aactgccctt gccatagcag aggccgcagg tacggtttgg ggcggtaaaa aagtagaact
     7801 taacccgact aaatgggatt gggttaaaaa taccgattat aaaacacctg ctgcccgacc
     7861 tatgcagact ttagatgggg aaatggccgg tgggaataaa ccgcctaaat ctataacgtc
     7921 tggaggaaaa gccaatgctg caacttatcc tcaattagtt aatcaattaa ctgggcaaaa
     7981 cttaaaaaac attgcggctc aagatccaag attgagtcta gctcttcata agagtgaaaa
     8041 aaattttcca ataggaactg caacttatga agaggcagat aggctaggta aaatttgggt
     8101 tggtgagggt gcaagacaaa ctagtggagg cggatggtta agtatagatg gcactcgaca
     8161 atatcggcca ccaacagaaa aaaattcaca atttgcaact acaggtattc aagcaaattt
     8221 tgaaacttat actattgatt caaatggaaa aaggaataaa attaaaaatg gacatttaaa
     8281 tattaggtaa ttatatgaga ttaaaattaa aaaattatga ttgttctgac tttgatttgg
     8341 aagatttccc acaagacaag ttagaaaatt tttgtatact tttgacatta tcaataggtt
     8401 ttgatgaaag taatggtgct gattattttt atgtttatat atatagcact gaatggttac
     8461 tttcaaatat acatagaccg atgtcattaa aaaactcaat tgttacaaac cgttttaata
     8521 ttgaacacat attaaaattg ataaatgata tattggaaat atgtaattca acgtctgaag
     8581 ataagtctat atcaaatttg gcaaaatatt ttgactggga atttgacgat tataatctaa
     8641 atatccaaga ctaaagatgg tggacagcat ttagaccaaa aacaggcaaa cggtattttg
     8701 atgacgaatt aaaataaaag gaattattca tgaaattaat taattttgaa gagagttcag
     8761 ttaaaattag tctcgataaa gatgagttgt atattattca agcaattgta ggagaaattt
     8821 attcaggcgt atgcgtggat tgtagagatt ttgagataat tcatggtgtt gaaaaaataa
     8881 agttctttct ttggataaag aacttaaaaa aatatatgat acttgggata aatgttagct
     8941 aggtaaggtg ggcacttgtt gcccaccttt tgccgtatat taaaaatcct tttcaggctg
     9001 cctgaaactg ggtgggcatc aatgccaatc ctaactgtgc tgatgaagca ggaaaactga
     9061 tatgggaaaa cgaccccaat aaaaattggt gggctaaccg tatggatgat attcgcggca
     9121 tcgtccaagg tgcggttaat ccttttttaa tgggttttca aggagtaggg attggggcaa
     9181 ttacagacag tgcggtaagc cctgtcacag atacagccgc tcagcagact ctacaaggta
     9241 ttaatgattt aggaaaatta agtccggaag cacaacttgc tgccgcgagc ctattacagg
     9301 acagtgcctt tgcggtaaaa gacggcatta attccgccag acaatgggct gatgcccatc
     9361 cgaatataac agcaacagcc caaactgccc ttgccgtagc agaggccgca actacggttt
     9421 ggggcggtaa aaaagtagaa cttaacccga ccaaatggga ttgggttaaa aataccggct
     9481 ataaaacacc tgctgttcgc accatgcata ctttggatgg ggaaatggcc ggtgggaata
     9541 gaccgcctaa atctataacg tccaacagca aagcagatgc ttccacacaa ccgtctttac
     9601 aagcgcaact aattggagaa caaattagta gtgggcatgc ttataacaag catgtcataa
     9661 gacaacaaga atttacggat ttaaatatca attcaccagc agattttgct cggcatattg
     9721 aaaatattgt tagccatcca acaaatatga aagagttacc tcgcggtaga actgcgtatt
     9781 gggatgataa aacagggaca atagttatcc gagataaaaa ttctgacgat ggaggtacag
     9841 catttagacc aacatcaggt aaaaaatatt atgatgattt ataggaaaaa gccatgaata
     9901 tactatccat aaataatcaa aactcaacta tttcactaac tcaagatgaa gtttttgttt
     9961 tacgagctat cttgaatgag atatatgcgg gcgtatgtgt agattcaaga gaatttgaaa
    10021 atgtatctgg tgttagaaaa catgaagtag ataatttaca acaacagttt gctggaattt
    10081 ataaaaaaat gacaacttaa caacccaaat ttttgtcaga gcctagtgca aattacaact
    10141 atgattctat tgtagccgaa atgaaagaaa aaaatcatgg gttggcgaca gggttgatgt
    10201 tgttaatatg cctgatggag cacctactag tatggataac acgcgtatta tggcagcacg
    10261 tgaagcagga gtaaaagtgg aagcgaatgt tcataatttt aatgaccgat tatcatcaaa
    10321 agagagaatc aggtttaagc atgatggtat tgagcctcaa acttggggag aagctatcca
    10381 gctacgaatt agaaagcaag aaacacaaaa aggagttcca gaagggtgga gcaaaagatt
    10441 tcctaacgga agtatttatg atgtaaaggt acttaggaaa tgataaaaca aaatagtttt
    10501 gttccgtatc ctgaagcaat gcttcctaaa ggatttaaat atccgcaaag ttatttaaaa
    10561 ttagctcaat ccactcatgc cattaactac gatgaacaat attcttttcc ttggtggttt
    10621 gaaaatgcag aaagcaatat atcagaagta attgacattt attttgaaat aactggcatt
    10681 ccaaacctat taccttttgc tagaaaccaa gagtgggctg cctgttttga tatttcagat
    10741 aaatcaggta atcctaaaat tatagtagtt aatttagata atacaaaata ttacgagact
    10801 tttgaaaatt ttgatacttg gctaaaagaa gctgaaaatg atggttggta gcagccgtag
    10861 gtcggattct cgaatccgac attttccaac agcggcattt cggaaacgat agatgcgtca
    10921 aatatttttg tcggatacaa atatccgacc tacatctctg cgcagcaaac tttacaaga
//
LOCUS       DAWFAN010000061        10636 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWFAN010000061 DAWFAN010000000
VERSION     DAWFAN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.061"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(14..1216)
                     /gene="trpB"
                     /locus_tag="WGO65_001094"
     CDS             complement(14..1216)
                     /gene="trpB"
                     /locus_tag="WGO65_001094"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ0327393.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTAAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPQMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1299..1988)
                     /locus_tag="WGO65_001095"
     CDS             complement(1299..1988)
                     /locus_tag="WGO65_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ0327394.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLRERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            2185..2964
                     /gene="rsmA"
                     /locus_tag="WGO65_001096"
     CDS             2185..2964
                     /gene="rsmA"
                     /locus_tag="WGO65_001096"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ0327395.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVIEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNNIEGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGRADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKVV"
     gene            3137..3865
                     /locus_tag="WGO65_001097"
     CDS             3137..3865
                     /locus_tag="WGO65_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0327396.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENIALAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            3938..4297
                     /locus_tag="WGO65_001098"
     CDS             3938..4297
                     /locus_tag="WGO65_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327397.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMSLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            4302..4748
                     /locus_tag="WGO65_001099"
     CDS             4302..4748
                     /locus_tag="WGO65_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ0327398.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRKLKVWVLWRQPKKTAETDTSD"
     gene            4779..6053
                     /gene="folC"
                     /locus_tag="WGO65_001100"
     CDS             4779..6053
                     /gene="folC"
                     /locus_tag="WGO65_001100"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ0327399.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPKSLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACALTVLECLNAKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALHRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6066..7091
                     /gene="ftsN"
                     /locus_tag="WGO65_001101"
     CDS             6066..7091
                     /gene="ftsN"
                     /locus_tag="WGO65_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0327400.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLESSAKPKQEEAAEKPQQAETAKTAPKQAKQRAAEKVPATADS
                     TDTVAVEKPKRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSD
                     SAVKEAKKADKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGY
                     AEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGI
                     AGQVTNE"
     gene            7084..7581
                     /locus_tag="WGO65_001102"
     CDS             7084..7581
                     /locus_tag="WGO65_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ0327401.1"
                     /translation="MNNLPVADLLVSAIIAACIVISTMRGVIAEAGSMAAWVVSFFFA
                     KLFAASFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLKHADNRDG
                     ALEDD"
     gene            7790..9334
                     /gene="purF"
                     /locus_tag="WGO65_001103"
     CDS             7790..9334
                     /gene="purF"
                     /locus_tag="WGO65_001103"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ0327402.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDNEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTIITRQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDDIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRVEHSISIS
                     DAGDEE"
     gene            9434..9925
                     /gene="greB"
                     /locus_tag="WGO65_001104"
     CDS             9434..9925
                     /gene="greB"
                     /locus_tag="WGO65_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ0327403.1"
                     /translation="MSTETKNYITPAGWQALKDELYRLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            9984..10610
                     /locus_tag="WGO65_001105"
     CDS             9984..10610
                     /locus_tag="WGO65_001105"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ0327404.1"
                     /translation="MRKIRTKICGITTLEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTTSDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         2652 a         3088 c         2754 g         2142 t
ORIGIN      
        1 cagacggacg aggttacagt ttaatccctt tgagcttcgc cacggtattg atgtctttgt
       61 cgccacgacc tgagaggttg accaaaatca cttggtcttt gcccatttgc ggcgcgtttt
      121 tcaccgccca agcaacggca tggctggatt cgagagcagg aataatgccc tcgaagcggc
      181 agagcaagtc aaaggcttcg agtgcttcgt cgtctttggc ggcggtgtat tcgacgcgct
      241 tgatgtcgtg cagatggctg tgttccgggc cgatgccggg gtaatccaag cctgcggaaa
      301 cagagtgcgt acccaaaacc tggccgtttt cgtcctgcat cagatagctg cggaaaccgt
      361 gcaatacgcc aatcggtgcg cccgaagtaa tcggcgcggc gtgatcgggg gtgttcacgc
      421 ccaaaccgcc ggcctccacg ccgacgaggc gcacgttttc ttcgccgata tacgggtgga
      481 acaaaccgat ggcgttcgat ccgccgccca cgcaggcaac ggcaacgtcg ggctgtctgc
      541 cgatggcttc caacatttgt tctttggctt cattgccgat cacgcattgg aaatcgcgca
      601 ccatttcggg atacggcgcg gggccagcgg cggtaccgat gatgtagaac gtgtcgtcca
      661 cgcgggcgac ccattcgcgc atggcttcgt tcatcgcgtc tttcagcgtg cggctgccgc
      721 tttctacacc gaccacgttc gcacccaata atttcatacg gaacacgttg ggcatttggc
      781 gttggatgtc gtccgcgccc atatacacgt cgcaagtcat accgaagcgt gcggcaacgg
      841 tggcactcgc cacgccgtgc tgacccgcgc cggtttcggc gatgacgcgt tttttaccca
      901 tgcgttttgc caacagtgcc tgaccgatgg tgttgttgac tttgtgcgcg ccggtgtggt
      961 tcaagtcttc gcgcttcaac cagatttgcg cgccacccag atgttcggac aaccgcgcgg
     1021 cgtggtaaac ggggctgggc ctgccgacgt agtgtttcaa atcgcggtgg aattcttccc
     1081 aaaacgacgg gtcgtttttc gctgccttat aggcatccgc cagctcttgc aaagcgggaa
     1141 tcagggtctc ggacacgaac agcccgccgt gttcgccgaa aaagcccttc tcgtcgggcg
     1201 cgtggtagtt cttcatcgga ttcttccttg tgcttcaggt tttcagacgg catttccgtt
     1261 ttttcaaaca tgccgtctga acgggaaatt gtcggcaatt ataactgttt ttcacacaag
     1321 cccgccattt caaaaaacgc ccgatacgcc gccgcttttt tcgccaaact caaaggatag
     1381 gcgcgcgaag tcgaaggcag gcgcgtcagc gtcaaatccc gtccggcaaa cggaaacggc
     1441 acggtttcgc ccgttttcgg cattttgata ccgccaccct gaatatcgag caggatttcc
     1501 gtcgccttgc cgcctgtcgt acaaatatgg cggcactcgg gtatttttgc caacaccgcc
     1561 gccaaatcga cggtttcaac tacctttaaa aacttgtcgg acgcattgcc gtgctgacgt
     1621 accgccttca acacggtcgg acaggacgca attccccgtt cgcgcaaaaa cgccttgatt
     1681 ttctcggcat caaacgcttt ttcagacaac ctttggaaat gcgccgcatc attaaaaaac
     1741 accagcccgt aaacgcgcca catatcgttt tggaaattcg gataatgaaa ctgcatcgcg
     1801 cgtttgtctt ccttgggcgg aaacgtcccc atcatcatga ccgcagcctt cggcggcaac
     1861 acggaatcga agggatgggt ttcgatttca agcagatttt cagacacgtt caatcctcca
     1921 acaggcaaaa cggcagacag ccggtttttg caccaaaccg ccaaacacgg caagccttca
     1981 aacagcatta tcacgccctg ccgttttcgg ttacaatgcc gacatcaaac ggatactgta
     2041 aacacattta tgtttcaaca cacaggacga cacttaaagc accgccctat gtgccatcct
     2101 gatttggaag gggttacgcc cctcccaaat agagtctgat tctaccgccc taaagggcgg
     2161 ggtttcaacc gaaaaggaaa cacgatgaaa gaacacaaag cccgcaagcg tttcgggcag
     2221 aattttttgc aggacacgcg gattatcagc gatattgtca acgccgtgcg cccgcaggcg
     2281 gatgatgtcg tgattgaaat cggcccgggt ttggcggcga ttaccgaacc tttggcgaaa
     2341 aaactgaacc gcctgcacgt tatcgaaatc gaccgcgaca tcgtatgccg tctgaaaacg
     2401 ctgccgtttg cggataaact cgtcattcat gaaggcgacg tgttgcagtt tgatttcaac
     2461 aacattgaag gcaaaaagaa aatcgtcggc aacctgccgt acaacatttc cacgccgctt
     2521 ttgttcaagc tggcggaggt ggcggacgat gtcgtcgata tgcactttat gctgcaaaaa
     2581 gaagtggtcg agcgcatggt tgccgcgccg aaaagcaacg actacgggcg tttgggcgtg
     2641 atgctgcaat atttcttcga tatggaaatg ctgattgacg tgccgcccga atcgttcgac
     2701 cctgcgccga aagtggattc cgccgtcgtc cgtatgattc cggtgaaaca ccgcatcggc
     2761 agggcggacg atttcgagca ttttgccaaa ctcgtgaaac tcgccttcca ccaacgccgc
     2821 aaaaccatac gcaacaatct gaaagagctt gcaggcgacg atgatttgca ggcagtcggc
     2881 atcaatccgc aagaccgcgc cgaacatatc gcgccagaga aatacgtggc gttgagcaat
     2941 tatctggcgg gcaaggtcgt ctgaaaaggg agtaagccga tgacgaaata cctgctttta
     3001 cccctgatac tgccgcttgc agcctgcctg accaatccga acgaccgttt ttttgacggc
     3061 agacgctaca cgcttcatca tatggagctc ccgtaacgac aacctgcaac ggcaaatgcc
     3121 gtctgaaaga aaagacatga tcaaattcaa aaacgtacac aaacatttca aagacctgca
     3181 cgtcatcaac ggcgtgaact tggaaatcaa aaaaggcgaa gtggtcgtcg tctgcgggcc
     3241 ttcgggcagc ggcaaatcca cactcatccg caccgtcaac cagcttgaaa gcatcgaaag
     3301 cggcgagatt tgggtggacg gtgtcaacgt tgccgaccca aaaaccgatt tgaacaaaat
     3361 ccgcgaagaa gtcggctttg tgtttcaaag tttcaacctc tacccgcacc tgaccgtgtt
     3421 ggaaaacatc gccctcgcac cgatgaaggt taaggggcaa aatgcggaac aggcggagaa
     3481 aaaggcaatg gagcttttgg aacgcgtcgg actggcacac aaaaaagacg ccttcccttc
     3541 ccaactttcc ggcggccagc agcagcgcgt ggcgattgca cgcggtttgg cgatggaacc
     3601 gcgcgtaatg ttgtttgacg aaccgacctc cgcactcgac cccgaaatgg tcggcgaagt
     3661 gttgaaagtg atgaaagact tggcagaaag cggcatgacc atgatgtgcg taacccacga
     3721 aatggggttt gcccgcgaag ttgccgaccg cgtgattttc gtcgataaag ggcaaatcct
     3781 cgaagacgaa acgcccgaag cgttttttac caacccgaaa cacgaacgcg ccaagcagtt
     3841 cctgcaacag gtcatgaccc actgatgcta taccgtctga aaaccgaacg ttcagacggc
     3901 atagcccgac accgcaagat gattgagaaa gaaaaccatg tattttgttg accgcaccgc
     3961 cgtcgtcctc aagccgaccg tccgcttttt ggaatggctc aaaagcaccg acgaaaatat
     4021 gcccgacctg accatagagc agcttcgtgc caactgctcc gtcttcctcg ttccgcagtt
     4081 cgacgagccg gaagccgtcg tttcctattt cgacgaacgc taccgccaga ttttcgaggc
     4141 ggagctggca ggttgggaca tcgataagga taaatggccg caagatatga gcttgaaagc
     4201 gttttgggag tttttcgaca tcgaaatcca cgatatggtt ttagacatgg aagaagcgga
     4261 attgaacatt acccccgtgt tcgacaacat gatgtaaggc tgtgcgcgcc tttcagacgg
     4321 cattgaggcc gtcccgcatc ctgaacatcc tgaccgtatc gctccactcg gcttcccttg
     4381 ccgtctgcct gacctggttt tacggccgga tgatgtggtt cggtttggcg gctttggcgg
     4441 caagttacgc atactcgctc aggataacga atctgaaaca ccgccatgcc ataaccgcca
     4501 ttaccataga ccgcgacgga caggcggaaa tcgtatccgg caaagaaaaa acggcaaagg
     4561 cggcggcact gtcgggcagc agtatggtta cgccttatgc cttattctta caatgggaca
     4621 ccggcggcaa aaccgtccgc cactgcatca cccacgatat ggcggacccg gaatcctacc
     4681 gcaagctgaa agtctgggta ctttggcggc agccgaaaaa aaccgccgaa accgatacat
     4741 cagactgatt tcagacggca tttgaaaacg aaacactcat gaaaacacta caagactggc
     4801 tctcccattt ggaaaccgcc cacagcggcg gcttgatcga tatgggcttg gaacgcgtaa
     4861 gcgaagtgaa aaaacggatg aagctcacgc cgcaatgccc cgtcgtcgtt gtcgcgggaa
     4921 caaacggcaa aggttcggtc tgcgcctacc tgacacagat ttacaaacag gcgggctaca
     4981 aaaccggcac actgaccagc ccgcatttat tgcgttacaa cgaacgcatc gccatcaacg
     5041 ccgaacccgt ttcggacgat acgattattg cctctttcga gcgcatcgaa gccgcgcgcg
     5101 gcgaaatatc actgacttat tttgaattca atacgttggc ggcggtcgac atcttcatgc
     5161 gcgaacaggt tgacgtgatg atattggaag tcggcttggg cggacgcttg gacgcggtca
     5221 acgcgtttga cggcgattgc gcggtggtta ccagcgtgga tttggaccat caggcatttt
     5281 tgggcgatac ggtcgagcag gtcggctttg aaaaagcagg cgtgttccgc agcggcaaac
     5341 ccgccatctg cggtcaaaac cccgcgccca aatcgttgct ttcacacgcc gaagccatag
     5401 gcgcgaaact gctgatggtg cagcgcgatt tcgagtttca cgcgatggaa aacatccaat
     5461 ggaactaccg cttccggccg caacattcag acgaccccgc acgcaaccgc aatgccctgc
     5521 ctttccctgc attacgcggc acataccagc tttccaacgc cgcctgcgcg ttgaccgtat
     5581 tggaatgctt gaacgccaaa ttgcctgtgg acatcggcgc catcaaacgc ggattgctgc
     5641 tggttgaaaa tcccggacgc ttccaagtcc tgcccggccg tccgctgacc gttttggatg
     5701 tcggacacaa tccccacgcc gcccgcgccc tgcaccgcag cctgattaat ttggcgtttg
     5761 cgcaaaaacg caccgccgta ttcagtatgc tgtccgacaa agacatagac ggcgtgttgg
     5821 aaatcgttaa agaccagttt gacgaatggt atatcgcgcc tttggacgtg ccgcgcggca
     5881 tgacgctcga gagcctgcaa aacaaactgc acgaacacag catcgaaaac atacagacat
     5941 tcgccgccgt ccgcgaagca taccgggcgg cagcctcaaa agcgggcgag aacgacagaa
     6001 tcgtcgtttt cggctcattt cataccgttg ccgatgtcat gtcggtactg taaaaggaaa
     6061 cttctatgtc cgaaaacaaa caaaacgaag tcctgagcgg ttacgaacaa ctcaaacggc
     6121 gcaaccgccg ccgcctcgta acggcaagtt gcctggttgc cgcctcctgc atcctgctgg
     6181 cagccgccct cagttccggc cctgccgaac agactgccgg cgaaacaagc ggcgtagaaa
     6241 acaaagcggc aggtgcggca caaacccctg ccttgaaatc cgccgccgac aaaccgcagg
     6301 acttggcagg cgaagacaag ccttctgccg ccgacagcga aatcagcgag cctgaaaacg
     6361 taggcgcgcc gctggtgctg attaacgacc gcctcgaaga cagcaatatc aaaggcttgg
     6421 agtcatccgc aaaaccgaaa caggaagaag ctgccgaaaa accgcagcag gcagaaactg
     6481 ccaaaaccgc accgaagcag gcaaaacaac gcgctgccga aaaagtgccg gcaactgccg
     6541 acagtacgga tacggtagcg gttgaaaaac cgaaacgcac tgccgaaaca aaaccgcaaa
     6601 aagcggaacg cactgccaaa gccaagccca aagccaaaga aaccaaaacc gccgaaaaag
     6661 ttgccgacaa accgaaaact gccgccgaaa aaaccaaacc ggatacggca aaatccgaca
     6721 gcgcggtaaa agaagcgaaa aaagccgaca aggctgaaag caaaaaaaca gccgaaaaag
     6781 accgttcgga cggcaaaaaa cacgaaacgg cacaaaaaac cgacaaagcg gacaagacca
     6841 aaaccgccga gaaggaaaaa tccggtaaaa aagccgccat tcaggcaggt tatgccgaaa
     6901 aagaacgcgc cttaagcctc cagcgcaaaa tgaaggcggc gggtatcgat tcgaccatca
     6961 ccgaaattat gaccgacaac ggcaaagttt accgcgtcaa atcaagcaac tataaaaacg
     7021 caagggatgc cgaacgcgat ttgaacaaat tgcgcgtaca cggtatcgcc ggtcaggtaa
     7081 cgaatgaata acctccccgt tgccgacctc ctcgtctccg ccatcatcgc cgcctgcatc
     7141 gtcatttcca cgatgcgcgg cgtgattgcg gaggcaggtt cgatggcggc atgggtggtt
     7201 tccttctttt tcgccaaact ctttgccgcc tccttcgccg acctcgcctt tgcctcgttc
     7261 caaccccgcc tgtttgcatt ggctctgtcg ttcatttccc tgttcgtcat tgcctgtctg
     7321 atccagaaaa tgctccgttc gctgctgacc ggcgcagttt cggcggtcgg tttgggcttt
     7381 gccaaccgca ttttgggcgg cgtattcggt gcattgaaag gcgttttgat tgttaccctg
     7441 ctgatcatgc ttgcttcaaa aaccgacctg cccgataccg aagaatggcg gcaatcttac
     7501 acactgccgt ttttcgtatc gctttccgaa gcagtgttaa agcatgcgga caacagggat
     7561 ggagctttgg aagacgattg attgccatac ggatatttca gacggcattc tcttttatgc
     7621 cgtctgaaat gtttttttcc tttcctcagc catatcacag attaaatctt atcctccata
     7681 aacccaccta tctgtccttc gctcaaatat tgtcaacaat tcaggcaaaa aaccaccgtt
     7741 tgctttataa ttccgttcta cccttaaacc tcgaatcgga gaagacgata tgtgcggcgt
     7801 attaggtttg gtcagtcatg aacccgtgaa ccagcttctg tacgacggtt tgcagatgtt
     7861 gcagcacagg gggcaggatg cggcgggcat tgcaacggcg gaaggcggca ccttccatat
     7921 gcacaaaggc aaagggatgg tgcgcgaagt gttccgcacg cgcaatatgc gcgatttggt
     7981 cggcaacgcc ggcatcgccc acgtccgtta tcccacggcg ggcaacgccg gcagcagcgc
     8041 ggaggcgcag cctttctacg tcagctcgcc gttcggcatc gttttggcgc acaacggcaa
     8101 cctcaccaac actgccgaac tgtatgaaaa cgtgtgcaac aaacacctgc gccacgtcaa
     8161 caccagctcc gattccgaag tcttgctcaa cgtattcgcg cacgaattac gccgcgaagt
     8221 ctctaaaaac gccgacccgc accggctcaa tgccgacaat attttcaacg ccgttgccga
     8281 agtccaccgc ctggtgcgcg gcgcatacgg cgtggttgcc atgattgcgg gctacggtat
     8341 gcttgccttc cgcgaccctt acggcatccg cccgctggta ttgggttcgc aaaccgacaa
     8401 cgaaggcagg aaatcctatg cagtcgcctc cgaatccgtc gccttcaacg cgcttaccta
     8461 cgatttggaa cgcgatatcc ggccgggcga agcggtattt gtcggctttg acggcacaat
     8521 catcacccga caatgcagcg accgcgccaa actcagcccc tgcctttttg aatatgttta
     8581 ttttgcccgc cccgactccg taatcgacgg cgtatccgtt taccagtcgc gcttggatat
     8641 gggcgtgtcc ttggcggaaa aaatcaaacg cgagctgccc gtggacgaca tcgatgtcgt
     8701 gatgcccatt cccgacacca gccgccccag cgcgatggag cttgccgtcc acctcgacaa
     8761 accctaccgc gagggcttga ttaaaaaccg ctatatcggc cgcaccttta ttatgcccgg
     8821 ccaggcgacg cgcaaaaaat ccgtgcgcca aaaactcagc ccgatggaaa ccgagtttgc
     8881 aggcaaaagc gtgttgctgg tggacgactc catcgtgcgc gggacgacca gccgcgaaat
     8941 cgtcgaaatg gtacgcgcgg cgggcgcgcg caaagtctat atcgcctccg ccgcccccga
     9001 agtgcgctat cccaacgtat acggcatcga tatgcccacg cgcgaagagt tgattgccaa
     9061 cgggcgcagc gcggcggaaa tcgccgccga aatcggcgcg gacggcatcg tgttccaaaa
     9121 tttgagcgat ttggaagccg tcgtcaaagc actcaatccg aaaatcgaat ccttcgattc
     9181 gtcctgtttc aacggcattt atcagaccgg cgacatcgat gatgcctacc tcgaccgcct
     9241 gtctgccgag aaatccggct gcggcggcct gaaaatccac ccgagcaggg tggaacacag
     9301 catcagcatc agcgatgcgg gcgacgaaga ataaggcttc ccaaaccgcg atgccgtctg
     9361 aaatccgaac gccgggtttt cagacgacat ttcccatatc ggcatcacat ttcccatttc
     9421 atccgcacgc aatatgagta ccgaaaccaa aaactacatt acacccgccg gctggcaggc
     9481 attgaaagac gaactttacc ggctggtcaa caaagaacgc cccgaaatcg tccaaatcgt
     9541 caattgggcg gcaggcaacg gcgaccgcag cgaaaacggc gactatcttt acggcaaacg
     9601 ccgtatgcgc gaaatcgacc gccgcatccg tttcctgacc aagcgtttgg aggctgccgt
     9661 cgtcgtcgat cccgaattgc gcgaagcgac cgaccaagta tttttcggcg cgacggtcgg
     9721 gctgttgcgc ggcgacgggc gcgagcaaac cgtcaaaatt gtcggcatcg atgaaatcga
     9781 taccgcgcaa aacaaaattt cttggatttc gccgttggcg cgttgcctga ttaaagcgcg
     9841 cgaaggcgac gaagtggttt tgaacacgcc ggaagggcgt gaggaaattg aaatcctttc
     9901 tgtcgaatac atcaaaatcg actgatcggg aatgccgtct gaaacggcac aaacgcatcg
     9961 tttcccaaat aaaggaatac cgaatgagga aaatccgcac caaaatctgc ggcatcacca
    10021 cactggaaga cgcagcggcc gccgcagcgg caggtgcgga tgccgtcggg ctggtctttt
    10081 tccaaggcag cagccgggcc gtcgatattg cccgcgcaaa aaaaatcacc gccgcactgc
    10141 cgccgtttgt cagcgttgtc gcccttttcg tcaacgaaag cgcgcaaaac atccgccgca
    10201 tccttgccga agtgccgata cacatcatcc aattccacgg cgacgaagac gacgcattct
    10261 gccgccagtt cgaccgcccc tatatcaagg ccattcgtgt tcagacgaca tcagacatcc
    10321 gaaacgccgc cgaccgcttc cccgacgctc aggcactgct tttcgatgcc taccatcctt
    10381 cggaatacgg cggcaccgga caccgcttcg actggacgct gctggcggaa tattcgggca
    10441 aaccgtgggt acttgccggc gggctgaccc ctgaaaacgt cggcgaagcc gtccgcatca
    10501 ccggagcgga agcggtcgat gtatccggcg gcgtggaagc gtctaaaggc aaaaaagacc
    10561 cagccaaagt cgccgccttt atcgcaaccg ccaaccgcct atcccgttaa agcaacaaaa
    10621 attgccgccg gaatga
//
LOCUS       DAWFAN010000062        10601 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWFAN010000062 DAWFAN010000000
VERSION     DAWFAN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.062"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(158..1267)
                     /gene="mltB"
                     /locus_tag="WGO65_001106"
     CDS             complement(158..1267)
                     /gene="mltB"
                     /locus_tag="WGO65_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ0327405.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1176..1427)
                     /locus_tag="WGO65_001107"
     CDS             complement(1176..1427)
                     /locus_tag="WGO65_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327406.1"
                     /translation="MPLDGIFGGGRQISGGAKGNARHSAKPPIAADTAFRAAPLRRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            complement(1468..2895)
                     /locus_tag="WGO65_001108"
     CDS             complement(1468..2895)
                     /locus_tag="WGO65_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0327407.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3081..6779
                     /gene="mfd"
                     /locus_tag="WGO65_001109"
     CDS             3081..6779
                     /gene="mfd"
                     /locus_tag="WGO65_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0327408.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHTLPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYMGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     NIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINELDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYRLAGADKLRF
                     TAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            6928..7311
                     /locus_tag="WGO65_001110"
     CDS             6928..7311
                     /locus_tag="WGO65_001110"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ0327409.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7333..8175
                     /gene="kdsA"
                     /locus_tag="WGO65_001111"
     CDS             7333..8175
                     /gene="kdsA"
                     /locus_tag="WGO65_001111"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ0327410.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCTHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPYQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEADNGKL
                     ILCERGSSFGYDNLVVDILGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8190..8630
                     /locus_tag="WGO65_001112"
     CDS             8190..8630
                     /locus_tag="WGO65_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ0327411.1"
                     /translation="MMQTFRKISRYVATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAILTRLQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKDAV"
     gene            8678..9964
                     /gene="eno"
                     /locus_tag="WGO65_001113"
     CDS             8678..9964
                     /gene="eno"
                     /locus_tag="WGO65_001113"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ0327412.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYNLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            9979..10257
                     /gene="ftsB"
                     /locus_tag="WGO65_001114"
     CDS             9979..10257
                     /gene="ftsB"
                     /locus_tag="WGO65_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ0327413.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10298..10588)
                     /locus_tag="WGO65_001115"
     CDS             complement(10298..10588)
                     /locus_tag="WGO65_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ0327414.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         2541 a         3091 c         2707 g         2262 t
ORIGIN      
        1 tgcaaaaatg ccgtccgaac cgaaacgtgg ttcagacggc attttatagt ggattaacaa
       61 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggcaag gcaaggcaac
      121 gccgtactgg tttaaattta atccactata aaaaacatta caatcccggg ccgccaagcg
      181 aattggcaat gtccctgacc gccgttacat acatccgact gtgattgtac tgccataccg
      241 tataaaaatt gttcaagccc aaataatatt caaacacgcc gggtgcggtt tccagtttga
      301 acaaaaccgc cttttcatca tctgcaagct cttcgccggg gatgatgccg tacgccttta
      361 aatccgccac cgtccgcgtc agggcggttt tttcgccaat gattgcctga acatccgcac
      421 ccggcgccaa tgttgcagac accagtattt tcccgcccgt gcgccaaccg tgctgcttca
      481 tataattggc aaccgatgcc gcgacatcgc cgatgttgcc ccatatgtcc cgatgtccgt
      541 ccccgtcata atccaccgcc catttccggt agctcgaagg cataaattgc ggcatcccca
      601 ttgcgcccgc atagctgcct ttaaaggcga aaacatcgcc gccttcttct tttgccagct
      661 ttaaaagctc gaccaattct ttttggaaaa acccggcgcg gcgggggtaa tcaaagccta
      721 aggtcgccaa tgcgtccgcc acacggaaac tgcccgtatt tttgccgtaa ttcgtttcaa
      781 tcccgataac cgccacgata agttcggcag gcacgccgta tttttgcgcc acatcatcga
      841 taagcgcgcg gttttccgca taaaaccggc gcgcgccgcg aaatttcgcc ttgcccgaat
      901 ttcccgtgcg gaacacatac cacggacgcg atgtggaggg gcggtgcata atcttgacga
      961 tgtccgcctt gtaagccgct ttgtcaaaaa aatcctgcca ttccgcccgg gaaaaatccc
     1021 ctttcccgac ttcatcgtcc acaaaacggc ggacatttgc attggcggca aacccgctgt
     1081 cggataccgg tacggctgcc gcgtcaaata cggctgccgc gtcaaacgcg gggcggcttt
     1141 cttttttcat ttcatccgcg cggggggctt gggcttcatt tgcccggggc gggcgtgcct
     1201 ccatcgccgt acaggcagac aaagccgcca aacaaattgc cagcggcagt atttttctct
     1261 ttttcataaa tatgttccga acaaataggg taagtgggaa agcggcgcaa gggcgcggcg
     1321 cgaaatgcgg tatccgccgc aatcggcggc tttgcagaat gccgcgcgtt gccttttgcg
     1381 ccgcccgaaa tctgcctgcc gccgccgaaa atgccgtcta aaggcacttc ccctttcaga
     1441 cggcattgcc tgcgccgtat ttgcccgcta tccgcaatat cggcagtcca atatgtcttt
     1501 gcggatatcg ttcagcaggc aggctgccgc gtcttcgtgt tccgcctgta cgaagacaaa
     1561 gagtcgggcg atgattttgc cgataaagac tttttgcagg gcgcaggcgt cggtcagcgt
     1621 gtgttcctgc aggaagctgc ggatgtcttc gggtaaggtg taatcacggg caacggcggc
     1681 aaagcgggca tcggtttgca ggcggtcgag cagggtttgg tttttgtcca acttcatgcc
     1741 tgcttctttt tcttcggcgg tggcgcggac aaactggtcg taaatttcgg cataaagcat
     1801 atcgttcggg ccttcaaaaa tcgtgaaggg gcggatgtcg atagcgatat tgccggcggt
     1861 gtgtccgcgt tcaaaaccct tcgcgcccaa gagtttttgc aacatttgcg cggcggcgta
     1921 agtgtattcc gtggcgaggg ttttgacgat gttcgcctcc atcagctgat gggcgacggg
     1981 ggcaacaggc gaaacggaat ggcagacgta gcggtaaaga atctcggaaa cctgatggcg
     2041 gcgccggatt tcgcggcgtt cgtaatcgac gaatctgatg tcgttgcgga cgtatcgttc
     2101 caggttttca aggatgtatt ccataatgcc gtgcgtcatg ccgatcagtt gcaggcggct
     2161 gcggataaag atgttttgga acgcgcgcaa accggcagcg tcgctctggg agagtttcat
     2221 cacggcggtt gcaggcattt cggcatcgat gcggttgacg gcgtaacgga cggcgcgcaa
     2281 gccttcggat gcgagggttt cgcagcggat gtatgttttg gggacgagca gcaggtcgat
     2341 gactttggcg agtttgccgt ttttgcgttc tttggcggca acgaggagga agtcgctttg
     2401 cgagttgccc tgccagtatt ttgcggcgtt gacgtaaatg gtttgtccgt cggtatattc
     2461 gtagtaagac tgcatttctc gggcaatcgc cgcgccggag gtttcgggtt cggtaacgcc
     2521 caaaccaccg ccctcgcctt tgaaaaccat gtccaaaccc tgtgcgattt gcgcttcatc
     2581 gccgaactct tgcagtggct gcaacaccag cgcgccttcg atgccggtac gcagcgtaac
     2641 gggcacgccg taatgccccg caatccgcaa gacttcctga atttcaaact ggctgccctt
     2701 gcgcccgccg tgttttttgt cgaggaaggg caacagcaaa cccgcctgct tcaaggcaag
     2761 ccatttgtct tcgggcaggt atcgcatcag gtcgataccg tctgaaaaaa tgcggcggaa
     2821 tgcggattcg atgtgcttta aaaaagcagc cgtgtccata gttgacggct gcgcgctcgg
     2881 ttcggtgtgt atcatcggct tcctctgtcg gttcccatta atcggcggcc ggtcaaaccg
     2941 cctgccacag tttagagttg attttctaaa ctttaccaca aagtgcgccg ggcaacaatc
     3001 cgccgacctt tcagacggca tcgcgccccc ctcccgtgct aaaatgaccg tttgcatcac
     3061 tgtccgccga ttgccgcact atgacctacc ccatccccaa accccgtgaa aaatcccgtt
     3121 ggtccaatct ttcgcaaggc tcgctgccct tggctttggc gcgttatctg ccgcataagc
     3181 ggctcaaggt cgtgctgacc caagatgcgg aacaggcgct acgccttcag acggcatggc
     3241 ggtttttccg tccgcacgac acggcggtgt tcctgccgga ctgggaaacg ctgccttacg
     3301 agcgtttttc gccgcatcag gatttggtgt cggaacggct gtcggcgttg tggcagatta
     3361 agagcggcgc ggcggacgtg ttgttcgtgc cggttgccac ggcgatgcag aagctgccgc
     3421 ccgtgccgtt tctggcaggg cgcacgtttt ggctgaaaac agggcagact ttggatatag
     3481 gccgtctgaa aaccgatttg gtagatgcgg gctacaacca tgtttcccat gttgttgcgg
     3541 cgggcgagtt tgccgtgcgc ggcggcattg tcgatttgtt ccccatgggc agcgaaatgc
     3601 cctatcgcat cgatttgttt gacgatgaaa tcgacagcat caaaaccttt gatacggaca
     3661 cgcagcgcac catctccccc gtttccgaaa tccgcctgct gccggcacac gagttcccca
     3721 ccgacagcga ggcgcaaaag attttccgca gccgcttccg agaagaagtc gacggtaatc
     3781 cgaacgatgc ggctgtatac aaagccgtca gcaacggcca tttcggtgca ggcgtagaat
     3841 attatctgcc actgtttttt gaaaacgagc tggaaacgct gtttgactat atcggcgaag
     3901 atgcgctctt tgtctctttg ggcgatgttc atgccgaggc aaaccgcttt tggagcgacg
     3961 tcaaatcgcg ttacgccatg gcgcagggcg acgaaaccta tccgcctttg cttccacagc
     4021 atttgtatct ctctgctgat gtttttgcag gccgtctgaa aaactacgga caagtgctgc
     4081 ccgatgtttc cggcaaggaa cacaccctgc ctgaccttgc cgtcaaccgc caatccgacg
     4141 agccgttgca ggcattgaag gattttcaga cgacctttga cgggcggatt ctgctgtgcg
     4201 ccgaaagctt gggacggcgc gaaaccatgc tcggcttctt gcagcaaaac ggcttgaaag
     4261 ccaaacctgt gtccgactgg cagggctttt tatcggcgca tgagccgctg atgattacag
     4321 tggcgccgtt ggcttacggg tttaaactgg gcggactgca atcgccgaac caacagcaaa
     4381 ctacttccgc ctccgaagga gaaggcgatg cggttaccga ccaaaccgaa ttttccgcat
     4441 ccgcaacaaa ccctctccct agccctctcc cacaggagag ggaacaaagt gcagccgccg
     4501 tttcagacag tctgaaagca gccgccgttt caaccgaaag cagcctgccc cccggtaaaa
     4561 gtaatctgca cgggcaaatc caacagcaac ctgccccctc ccccgtgggg gagggttggg
     4621 gagagggcaa agcagttgcc gctcaaagtg ccatcgccgt catcaccgaa tccgatctct
     4681 accaatacgt cgcccgttcg cgcgtccaca accgccgcaa gaaacacgcc gccgtttcag
     4741 acggtctgtt gcgcgacctt gccgaaatca acatcggcga ccccgtcgtt cacgaagaac
     4801 acggcatcgg gcggtatatg ggcttggtaa cgatggactt gggcggcgaa accaacgaaa
     4861 tgatgttgct cgaatacgca ggagaagcgc agctttatgt gcctgtttca caactgcatt
     4921 taatcagccg ctactccggt caggcgcatg aaaacattgc cctgcacaag ctcggcagtg
     4981 gcgcgtggaa caaggcgaag cgcaaagccg ccgaaaaggc gcgcgatacc gccgccgagt
     5041 tgcttaacct ttacgcccga cgcgctgccc aatcgggaca caagtttgaa atcaacgagt
     5101 tggattatca ggcgtttgct gacggcttcg gctacgagga aaccgaagac caggccgccg
     5161 ccatcgccgc cgtgattaaa gatttgacgc aagcgaagcc gatggatcgc cttgtgtgcg
     5221 gcgatgtcgg cttcggcaaa accgaagttg ccctgcgcgc cgcgtttgtg gcggtgatgg
     5281 gcggcaaaca ggtcgccgta ctcgccccga ccacgcttct ggtcgagcag cacgcgcaaa
     5341 actttgccga ccgtttcgcc gatttccctg tgaaagtcgc tagcctttcg cgtttcaaca
     5401 acagcaaagc cactaaagcc gcgctggaag gcatggcgga cggcacggtc gacatcgtta
     5461 tcggcacgca caaactggtg caggacgaca tcaaattcaa aaacttaggt ttagtgatta
     5521 tcgacgaaga acaccgcttc ggtgtgcgcc aaaaagagca gctcaaacgc ctgcgtgcca
     5581 atgtcgacat cctcaccatg accgccacgc cgattccgcg tactttaagt atggcgttgg
     5641 aaggactgcg cgacttctcg ctgattacca ccgcgcccag ccgccgcctc gccgtcaaaa
     5701 cctttgtcaa accctttagc gaaggcagcg tgcgcgaagc cgtgttgcgc gaactcaaac
     5761 gcggaggaca ggtatttttc ctgcacaatg aagtagatac gattgaaaat atgcgcgagc
     5821 ggctggaaac cctgctgccc gaagcccgca tcggcgtggc gcacggacaa ctgcgcgagc
     5881 gcgagctgga acaagtcatg cgcgactttt tacagcaacg attcaacgtg ttgctctgtt
     5941 ccaccatcat cgaaaccggt atcgacattc ccaacgccaa caccatcatc atcaaccgcg
     6001 ccgacaaatt cggactggcg caactgcacc agcttcgcgg gcgcgtcgga cgcagccacc
     6061 accaagccta cgcctacctg ctcacgcccg aatacatcac caaagacgca gaaaaacgcc
     6121 tcgacgccat cgcggcagca gacgaactcg gcgcaggttt caccctcgcc atgcaggatt
     6181 tggaaatccg cggcgcaggc gaaatcctcg gcgaagggca gtccggcgaa atgatacagg
     6241 tcggcttcac gctctacacc gaaatgctca aacaagccgt gcgcgatctc aaaaaaggcc
     6301 gccaacccga cctcgacgcg ccgctgggca tcaccaccga aatcaaactg cacagccccg
     6361 ccctactgcc cgaaagctac tgccccgaca tccacgagcg tctcgtcctc tacaaacgcc
     6421 tcgccgtctg cgaaaccgtg cagcaaatca acgccataca cgaagaactc gtcgaccgct
     6481 tcggcctgcc cgaacaaccc gtcaaaaccc ttatcgaaag ccaccacctg cggcttatgg
     6541 caaaagaatt gggtatcgat gccattgatg cggccggcga agcggtaacg gtaaccttcg
     6601 gtaaaaacaa taatgtcgat ccaaccgaaa tcatcctgct gattcagaac gacaaaaaat
     6661 accgccttgc cggcgccgat aagctgcggt ttaccgcaga gatggaaaat atcgaggtca
     6721 gaatcaacac cgtaaaaaac gttttaaaaa ccttgcaaaa cagatgcctg cccaaataaa
     6781 gccgacaccg caatgccgtc tgaaacaccg ttttccttgt ccgaaagccg ccattatgaa
     6841 tttgaaggaa actccactat aatacggcat tcagatttcc agacggcatc gcgcccgtca
     6901 gaccgcacac aaaccaaaag gaaatacatg ttccgtacta tgcttggcgg aaaaatccac
     6961 cgcgccaccg taaccgaagc cgatttgaac tacgtcggca gcattaccgt cgatcaagat
     7021 ctgttagacg cggcaggcat ctaccccaac gaaaaagtcg ccatcgtcaa caacaacaac
     7081 ggcgaacgtt ttgaaaccta taccatttca ggtaaacgcg gcagcggcgt gatttgtctg
     7141 aacggtgctg cggcaaggct ggtgcagaaa ggcgatatcg tcatcatcat gtcttacgtc
     7201 caactctccg aacccgaaat cgccgcgcat gaacccaaag tcgtcttggt agacggaaac
     7261 aacaaaatcc gcgacatcat ctcctacgag ccgccgcaca ccgtgctgta accctgaaaa
     7321 cggacattga tcatggatat taaaattaac ggcattaccg tcggtaacga tgctcctttc
     7381 gtactgttcg gcggcatcaa cgttttagaa gatttggatt ccaccctcca aacctgtacg
     7441 cattacgtcg aagttacccg aaaactcggt attccctata tctttaaagc atctttcgac
     7501 aaggcaaacc gttcctccat ccattcttat cgcggcgtag gcttggaaga aggcttaaag
     7561 atttttgaaa aagtcaaagc ggaattcggc atccccgtca ttaccgacgt acacgaaccc
     7621 tatcagtgcc aacccgtcgc cgaagtgtgc gatgtcatcc agcttcccgc attccttgcg
     7681 cggcagaccg atttagtggt tgccatggca aaaaccggca acgtcgtcaa catcaaaaaa
     7741 cctcagttcc tcagcccctc tcaaatgaaa aacattgtgg aaaaattcca cgaagccgac
     7801 aacgggaaac tgattttatg cgaacgcggc agcagcttcg gctacgacaa cctcgttgtc
     7861 gatatactcg gtttcggcgt gatgaaacag acttgcggca acctgccggt tattttcgac
     7921 gttacccatt ccctgcaaac ccgcgatgcc ggttctgccg catccggcgg tcgtcgcgca
     7981 caggctttgg atttggcact tgcaggcatg gcaacccgcc ttgccggtct gttcctcgaa
     8041 tcgcaccccg atccgaaact ggcaaaatgc gacggcccca gcgcgctgcc gctgcacctt
     8101 ttagaagatt ttttaatccg catcaaagca ttggacgatt taatcaaatc acaaccgatt
     8161 ttaacaatcg agtaacacgg tttcgcctta tgatgcagac tttccgaaaa atcagccggt
     8221 atgtcgcaac cttgtggctc ggtatgcaga ttatggcggg ttatatcgcc gcaccggtgc
     8281 tgttcaaaat gctgcccaaa atgcaggcgg gcgaaattgc cggcgtattg ttcgacatcc
     8341 tctcttggag cgggcttgcc gtttggggcg cggtgttggc tgccgccttt gccatcctga
     8401 cccgactgca aaccgccctg ctgctttttt tattgtccgc ccttgccgcc aaccaatttt
     8461 tggttacccc cgttatcgag gcactgaaat acgggcatga aaattggctg ttgtcgtttg
     8521 taggcggatc cttcggaatg tggcacggta tttcaagcat gattttcatg gcaaccgccc
     8581 tactttcagc agttttaagt tggcggcttt ccggcaaaga tgccgtctga agccctccca
     8641 tttttttacc tcccttcact tcacttggag aacattcatg agcgcaatcg ttgatatttt
     8701 cgcccgcgaa attttggact cacgcggcaa ccccacagtc gagtgtgatg tattgctcga
     8761 atccggcgta atgggacgcg cagccgtacc gagcggcgcg tccaccggtc aaaaagaggc
     8821 tttggaactt cgcgacggcg acaaatcccg ttattcgggc aagggcgtat tgaaggcggt
     8881 cgaacacgtc aacaaccaaa tcgcccaagc cctcattggt atcgatgcca acgagcaatc
     8941 ttatatcgac caaatcatga tcgaattgga cggtactgaa aacaaaggca atttgggtgc
     9001 gaatgcgact ttggcggttt ctatggcggt tgcacgcgcc gctgccgaag actcaggcct
     9061 gccgctttac cgctacttgg gcggcgcagg cccgatgtcc ctgcccgtac cgatgatgaa
     9121 cgtcatcaac ggcggcgaac acgccaacaa cagcctgaac atccaagagt ttatgattat
     9181 gcccgtcggc gcaaaatctt tccgcgaagc gttgcgctgc ggtgcggaaa ttttccacgc
     9241 cttgaaaaaa ctgtgcgaca gcaaaggctt cccgaccaca gtcggcgacg aaggcggttt
     9301 cgcccccaac ctgaacagcc acaaagaagc cctgcaactg atggtcgagg cgaccgaagc
     9361 cgccggctac aaagcgggcg aagacgtatt attcgcattg gactgcgcct ccagcgagtt
     9421 ctacaaagac ggcaaataca acttggaagc cgaaggccgc tcctacacca acgcggaatt
     9481 tgccgaatat ctggaaagtc tggtcaacga gttccccatc atctccatcg aagacggcat
     9541 ggatgaaaac gactgggaag gctggaaact gctgaccgaa aaactgggcg gcaaagtcca
     9601 actcgttggc gacgacctct tcgttaccaa cccgaaaatc cttgccgaag gcattgaaaa
     9661 aggcgtggca aacgcactat tggtcaaagt caaccaaatc ggtactttga gtgaaaccct
     9721 gaaagccgtc gatctggcaa aacgcaaccg ctacgccagc gtgatgagcc accgctccgg
     9781 cgaaaccgaa gacagcacca ttgccgactt ggcagtcgcc accaactgta tgcagatcaa
     9841 aaccggttct ttgagccgtt ccgaccgcat ggcgaaatac aaccaactgc tgcgtatcga
     9901 ggaagaattg gcggaagccg ccgactaccc cggcaaagcc gcattctacc aactgggcaa
     9961 ataagaaaag gttaaggtat gaagtgggta actgtcgttt tatccttcgc acttgtctgt
    10021 tgccaataca gcctctggtt cggcaaaggc agcatcggac gcaacagcag tctgagagaa
    10081 cagattgccg ttcaagaaga aaaaaaccag acactcgccc tacgcaatca ttcccttgcc
    10141 gccgaagtct atgatttgga aaacggtcaa gaagccattt cggaaatcgc ccgggtagaa
    10201 ctgggttata tccaagacgg tgaaaccttt taccgactca tcaggcataa ccggtaatac
    10261 cgtcaaaaag ccgtccgaac caatgttcgg acggctttta tttcaacaaa ctgtcagaca
    10321 gcccctcatc ctcccccgac aaaccgcaat ccagcctgac atccccctcg acgcaacagc
    10381 agcacggcag tatctcgtcc cgccccaaaa aagccaaagg cggctcccga taagtaacgc
    10441 ttccctccaa aatcttcacc cggcacgatc cgcaatatcc gcttcggcac tgatattcca
    10501 ccatatgccc cgtccgttcc aagccttcca acagggtctc gccctccaag agctcaaaaa
    10561 aacccttatt cgtaccaatg cgcgccattt ccgaccaatc a
//
LOCUS       DAWFAN010000063        10434 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWFAN010000063 DAWFAN010000000
VERSION     DAWFAN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.063"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            140..322
                     /locus_tag="WGO65_001116"
     CDS             140..322
                     /locus_tag="WGO65_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327415.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(384..1385)
                     /locus_tag="WGO65_001117"
     CDS             complement(384..1385)
                     /locus_tag="WGO65_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ0327416.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1462..5295)
                     /locus_tag="WGO65_001118"
     CDS             complement(1462..5295)
                     /locus_tag="WGO65_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ0327417.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGNEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALIREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHNGVEFVELAGLDGKHPIIHCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKTRWEWLLANLDTPLADYKARYGAA
                     VHAAPEAKDDESCFIAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELADIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKVLRAAMIQTGFPAQNFAYKIGKLLPVGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVQGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5556..5652
                     /gene="ffs"
                     /locus_tag="WGO65_001119"
     ncRNA           5556..5652
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WGO65_001119"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5820..7511
                     /gene="dld"
                     /locus_tag="WGO65_001120"
     CDS             5820..7511
                     /gene="dld"
                     /locus_tag="WGO65_001120"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ0327418.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGRALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNQQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHGGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            7643..7750
                     /locus_tag="WGO65_001121"
                     /pseudo
     CDS             7643..7750
                     /locus_tag="WGO65_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7806..8177
                     /locus_tag="WGO65_001122"
     CDS             7806..8177
                     /locus_tag="WGO65_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ0327419.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8332..8880
                     /locus_tag="WGO65_001123"
     CDS             8332..8880
                     /locus_tag="WGO65_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0327420.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLAQTEINPELQAYA"
     gene            9010..10101
                     /locus_tag="WGO65_001124"
     CDS             9010..10101
                     /locus_tag="WGO65_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0327421.1"
                     /translation="MNAQTLIANVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGKVLAGTGMSNTVKHLAGIEKHNLQAERVEGGYTVNGALPWVSNIGEEHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLD
                     GAWAETERLAGLAWSGTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
BASE COUNT         2416 a         2875 c         2825 g         2318 t
ORIGIN      
        1 attttgcaaa ggtctctatt tgtactgtct tcggctcgcc gccttgtcct tatttaaatt
       61 taattcacta tatttgcccg tgcgtttgtg ataaattcgg cactgtattt gatatgattt
      121 taacaaatcg ggaggggcaa tgttttggta tgtaatcggc ttttgctcct ttgtcgtcgc
      181 gctgctgtcg ctgtgggtca atgtcggcgc attcggtatg caggaagacg ataccccgca
      241 atcggattat gaaaaacgtt tgggcttggg tgcgaagctg aaaaacaaaa atacgccgga
      301 tacggaagaa aagcagcaat aaagatatga aatgccgtct gaaagatttt cagacggcat
      361 ttgctttgaa gcatgggaga cagtcaaaca tttatctcat gctcaaatgc gttcaggatt
      421 cggcggactt catccgcatc cttaaccgtt cggacgcggt caaacagcgt ttgcgccggg
      481 tcgaacgttt ttttcatcat gcccaaccat tgcttaagac gggcgatcgg gtatttattg
      541 tttgcctctt ttgtcaggca cagctcgaaa aactgccgta tccatttgga aacttcggca
      601 aaatccgtgt ctttgaccgg cccgccgttc tcgtattgct tgatttgccg cgccaaatcg
      661 gggcggatga ccgcgccgcg accgagcatc acgctgttgc agccgctgat tgttttgatg
      721 ccgatatagt cttgcaggct gaaaacgtcg ccgttggcgg taacgggaat attgacgctg
      781 tctcggattt tccttatcca ttcccaatgc gccggcggtt cgtaaccctc ggctttggta
      841 cgcgcgtgta cggtcagtcc gcatgcgccc ccttcggcaa tcgcacaggc gcattccaaa
      901 gcccggcttt tgtcttcgta accgagccgc atttttgcgg tgagcggaat atgtgcgggc
      961 aaacgtccgc gcagcgtttt gacgatgtgg aatatcagtt ccggctcttt taaaaggatt
     1021 gcgccgcctt tgtgtttgtt gacggtgggc gcggggcagc cgaagttcaa atcgattttg
     1081 ttcgcaccga agcggacggc ttccagcgca ttcgccgcca tattgtccgc atcgctgccc
     1141 aaaagttgga cggtgcaggg cgtgccggaa aacgttttgt ttccgttggc gatttcgggg
     1201 acatatttta accatatgga tcgggaatgc acggtatggg taatgcgtac aaattcgctg
     1261 acgcattcgt cgtagccgcc aatacgcgtc agcaggtcgc gcatcacgtc atcgaccaga
     1321 ccctgcatag gggcaaggat gattcgggtt ttttgtttgg gttcggttgt ttgtccgtca
     1381 atcatgattc gggtaggtat ttgattcaaa caatgccgtc tgaaggcttt cggcgttcag
     1441 acggcatttc cgtgtccgtt gttacagcaa tactttttcc acgccgccgt tgttggcttt
     1501 tttgacgaac tcgtccagcc agttttcgcc gagaatgtgt ttcgccattt caatgactat
     1561 gtagtcggca ggcatattgt tgtcgtcggc gtagcggctc aagccttgca ggcaggcggg
     1621 gcaggaggtc agcattttga cgggttcgcc ctgcggcagc tctttgaggt ttttctcgat
     1681 ttcctcttgt ttgcggaact tgacctgagt ggcgatgtcg ggccgtttga cggcgaacat
     1741 accggactcg ccgcagcagc ggtcgcttaa gacgactttc tgccccatca ggctgctggc
     1801 catttgggtg gcgttcatgg ttttgatggg ggtgtggcag gggtcgtggt agaggtattg
     1861 ctgaccttgc acgccgtcga gtttcacgcc tttttcgagc aggtattcgt ggatgtcgat
     1921 gatgcggcag ccggggaaga tttcttcaaa gcggtatttt tcgagctggt cgtaacaagt
     1981 gccgcaactg acaacgacgg tcttaatgtc gaggtagttg agggtgtttg ccatacggtg
     2041 gaaagccacg cggttgttgg tgctcatttc ttcggcttta gccttgttgc cgcccgcgtc
     2101 ttgcggatag ccgcaacaca tatagccggg cggcaggacg gtttgtacgc cgacgtgcca
     2161 gagcatggct tgaacggcaa gtccgatttg gctgaacagg cgttccgaac cgcaacccgg
     2221 gaagtagaac acggcttcgg catcttcggg cgcggcgggg ttgcggatga tggggatgct
     2281 tttgccgtct tcgatgccca ataaggagcg cggtgttttg gcgggtacgt ttttgggcaa
     2341 aggacggttg atgaaatgga taacctgttc tttaattggg gctttgccga cggttgcctt
     2401 gggttcggct ttttgctttt tcgtgccgac gggcagcagt ttgccgattt tgtaggcgaa
     2461 attctgcgcg gggaagccgg tctgtatcat ggcggcgcga aggactttga tggttttcgg
     2521 gccggtcgcg tttaggaatg ccatacccat tgaagctgcc ggcgcaaagc gtttgtggcc
     2581 ggaatcggca aggtagttgc ggacggctac ggttacgtcg ccgaagtcga tattgacggg
     2641 gcagggtttg acgcagcggt ggcacacggt gcagtggtcg ccgatgtctg ccagttcttc
     2701 aaagtgtttg acggaaacgc cgcgccgggt ttgttcttcg tataagaagg cttcggttaa
     2761 cagccctacg ccgaggattt tgttgcgcgg actgtacagc aggttggcgc gcggaacgtg
     2821 ggtggagcag acgggtttgc atttgccgca gcgcaggcag tctttgacgg aatcggcaat
     2881 cgtgccgagg tttgattttt ccataatcag cgattccgcg cccaacagct cgaaggacgg
     2941 cgtgtaggcg ttgcgtaagt ccgagccttt catcagtttg tggcggttga aggtgtgttt
     3001 ggggtcgact tggtttttgt agtcccaaaa cggctgcaag tcttcatcgg tcaaaaattc
     3061 gagcttggtg atgccgatgc cgtgttcgcc cgaaatcacg ccgccgagcg aacgggcgat
     3121 tttcataatg cgttcgaccg aacggtaggc tgtctgaagc atttcggcat cgtctgagtt
     3181 gaccggaata ttggtgtgaa cgttaccgtc gccggcgtgc atatgcaggg cgacaaagac
     3241 gcggctgcgt acggttttgg cgtggatttt gcccaagcct tggataatct tggtatcggt
     3301 tttgccgctg aagatttcgg caagcggctt cattacgtct gctttgacag aaacgcgcag
     3361 gcggaaatcg cggaaagcaa taaagcaact ttcatcgtct ttggcttcgg gcgcggcgtg
     3421 gacggctgcg ccgtagcggg ctttgtagtc ggcaagcggc gtgtcgagat tggcgagcag
     3481 ccattcccaa cgcgttttga cggcggaaac gtgggctagg gcgtgtttgc cgcgttcgcc
     3541 caacagttcg gcggtcggca ggtcagtgcc cattttgtcg atggggagtt tgcccgaaag
     3601 atattgctcc aaggcggcac agagtttgag cttgttttgg atggaaagct caatgttgat
     3661 gcgttcgatg ccgtccgaat actcgccgag cctttcgagc gggatgacca cgtcttcgtt
     3721 aattttaaag gcgttggtgt gcttggcgat ggcggcggtg cggctgcggt cgagccaaaa
     3781 ggttttgcgc gcttcgggag acacggcgat aaagccttcg ccatcgcggg cgcgggcaag
     3841 ttcgcaaatg tgttcggcgg ctgcctctac ggcggcttcg tcgtcggaaa ccacgtccgc
     3901 cagcaagacc attttcggcc gtcccttgcc cgccgctttg gttgcgtagc cgacggcgcg
     3961 gacgtaacgc cagtccaaat gttccaaacc tgccagccga acgctgtcgt gggcgagcag
     4021 gaaatcgcgg atttcgacga tagaaggcgt agcggtggcg accgtgccga aaaactccat
     4081 acacacagtg cgcgtgtatt tcggcatttt gtgcaacacg aaagcaacgc cggtgatgat
     4141 gccgtccgtg ccttctttct gtacgccggg caggccgctc aagaatttgt cggtaacgtc
     4201 tttgcccaaa ccgactttgc gaaatttgtg gccgggaatt tccaaacgtt cggttttaac
     4261 gatattgagg ccgtctgaat ccagcgtgtg aacgtcgaac acggcggttt cttcgtcgtg
     4321 gattttgccg aaattgtggc gcacgcgctc gatgcgcagc cattcgccct gcgggttgac
     4381 catctgccag taggcgaggt tgtccaacgc cgtcccccac agcacggctt ttttaccgcc
     4441 cgcgttcatc gccacattac cgcccacaca ggacgcatcg gcggaagtcg gatcgacggc
     4501 gaacaccaaa cctgcctgat gcgcggtttc ttccacccgc cgcgttacca cgcccgcgcc
     4561 gcaatggata atcggatgtt tgccatccaa gcccgccagt tcgacaaact ccacgccatt
     4621 atgtttatcc agcttttccg tattgatgac cgcgctcatc gcgtccaaag gaatcgcgcc
     4681 gccggtataa cccgtaccgc cgccgcgcgg gataataacc aaatccagct cgattaaggc
     4741 gcgcaccaaa ggcgcgatct ctgcctcggt atcaggattg acgacgacaa acggatattc
     4801 gacgcgccag tcggttgcat cggtaacgtg cgttacccgc gccaatccgt caaacataat
     4861 attgtgcggc ttggtgattt tgctcaaacg ctccaaaatc tgccgccgct tttggctggt
     4921 ttcatcaaaa ctgctatcaa aacgctcgac tgctttttct gctgcggcaa ccaagacatc
     4981 cacttgccga ttatcgtcgc ggcgtttgcg gatttcgttc aggcggtggc gcatttcgcg
     5041 tatcaacgcg gcgcggcgtt tcggatgctc cagcaaatca tcgaccagat acggattgcg
     5101 cacgaccacc caaatatcgc ccaacacttc aaacagcatc cgcgccgaac gtccagtttt
     5161 acgctgtccg cgcaagtctt gcagaatctg ccacgcttcg ttgcccaaca gtcggatgac
     5221 gatttcgcgg tcggtataag aagtatagtt atagggaatt tcccgaatac gctgcggggc
     5281 ggtagtcgtg gtcatggtgt gtccgtgttt ggcagttttt atttgaatgc cgtctgaaaa
     5341 gtcggtttca ggcgagtttg aaaaggcgat aatgtagttc aatttttgat atttttcaat
     5401 gccgaaacag gaaataccct tatagaaaca aaataacgct ttgagaaatt gttgacaata
     5461 ttatcaaata taaattttag gtggtgtttc cggcgtatga ccgtatcaaa acggttgagt
     5521 ttttcagaaa acccgttata atgccgtttc tctgaggcgg gtctccccgc atggcaaatc
     5581 ggaacaccgg gtcaggggcg gaagccagca gcccactccg atgcgccagt gccgggggtt
     5641 tggcccgccg ccctatttga aacgccggag cttcgatgtt ccggcgttcc cgtatcgcac
     5701 cgatgccgtc tgaaagccgt tcagacggca ttttctttac tttgccataa cgtataacta
     5761 caatgggaaa acaatttcaa tgtgcatatg cacctgccga caattttaag gaaactgcca
     5821 tgagcgcatc acaattactg tcaagactga cccaaaccgt aggcgaaaaa tacatcatta
     5881 ccgaccccgc gaaaaccgaa caataccgcc aaggctaccg cttcggcgaa ggcagggcgt
     5941 tggcggtggt gcgccccgga agcattctgg aaatgtggaa aattctgcag gcgtgcgtcg
     6001 aagcagacgt gattgtgatt acgcaggcgg cgaataccgg actgaccggc ggttcgaccc
     6061 ccgacggcaa cgattacgac cgcgacatcg tgattgtgaa caccatgcgg atgaacatca
     6121 tccaaaccat caacaacaac caacaagtcg tctgcctgcc cggctcgacc ctgaaccagc
     6181 ttgaattgct gctgaaacct ttggggcgcg aaccgcattc ggtcatcggc tcatcctgta
     6241 tcggcgcgtc cgtcttgggc ggcgtgtgca acaactccgg cggcgcgttg gtacagcgcg
     6301 gcccggccta caccgaaatg gcgttgttcg cccaaatcaa cgaagaaggc aggttggaac
     6361 tggtcaacca cttgggcata gacttgggcg atacgccgga agaaatcctg accaacctgc
     6421 aaggtcatca ttatcaaaac aaagacatca aacaagacgc gggcaaagga cacgaccacg
     6481 cctattgcga acacgtccgc caagtggacg aaccgaccgc cgcgcgtttc aacgccgacc
     6541 ccgcgcgcca ttacgaagcc tcgggctgcg cgggcaaact gatggtgttc gccgtccgtt
     6601 tggacacctt cccgcaagaa aaacaaaccg ccgtgttcta catcggcacc aacgacatca
     6661 acgagctgac cgacatccgc cgcgccgcct tgggcgaatt tgaaagcctg cccgtttccg
     6721 gcgaatacat ccaccgccac gctttcgata ttgccgacgt gtacggcaaa gacacgtttt
     6781 acgtcatcaa aaaattcggt acgcaccaac tgccgaaatt atttgacttg aaggcgcgcg
     6841 tggacaggtt cggcaaaaaa gtcagcttcc tgcccaaaca tttttccgac aaggcaatgc
     6901 agttcgtcag caaattcctg cccgaccacc tgcccaaatc catgcgcgat taccgcgaca
     6961 aatacgaaca ccacctgatt ctgaaaatgg gcggaaaagg cgtggatgaa gcgcgcgcgt
     7021 tcttaaaaga atattttgca caccacggcg gcgcgttttt tgaatgcaac gccgaagaaa
     7081 cccaagccgc gatgctgcac cgttttgccg tcgcttccgc cgccatccgc taccgcgccg
     7141 tgcatgatga tgaagtggaa gatttggtcg cgctcgacat cgccctgcgc cgcgacgacc
     7201 gcgattggtt tgaaaaactg ccgccggaaa ttgacaataa aatcatccat aaattgtatt
     7261 acgggcattt tatgtgccac gttttccatc aggattacat catcaaaaaa ggcaacgact
     7321 gcatggcgtt ggaacacgaa atgctgcatc tcttagacca acgcggcgcg caatatcccg
     7381 ccgaacacaa cgtcggtcat ttgtatgaag ccaagcccgc gctcaaacag ttttaccgca
     7441 aactcgaccc gaccaacagc ttcaacccgg gtatcggcaa aaccagcaaa aagaaaaact
     7501 gggcggaata agcgcgtccg ctttgaaggc aggcaatgcc gtctgaaggc aaaacgccgt
     7561 tcagacggca ttgtcgggtt tgaagttccc cgtcgggtaa tcgctttcaa accgtatgcc
     7621 gacacacggg cgcgcccctc ccaaatatag tggattaaat ttaaatcagg acaaggcaac
     7681 gaagccgcag acagtacaga tagtacggca aggcgaggta acgccgtact ggtttaaatt
     7741 taatccacta taaagtctga tcctaccttc ccaaagggca gggtttcaac cgaaaaggaa
     7801 acacgatgaa accatacaaa atctacaccc atcccgccct gccgccccaa gccgtcaaac
     7861 aaggctggtc atggccgggg ctcttgttcg gcacgctgtg ggcgtgtttc aaaagaatgt
     7921 ggggtttggg gctggggctg acgggcgcga tattcgtgct ggctgtgttt gcccaactgg
     7981 tttacggcga cacgcccgcc acagactcgg cgtttaacgt attgggcttg gcggtttccg
     8041 tctggttcgg cgcaaagggc aacagccttt atgcacgcca ccttttatcg cgcggctata
     8101 ccgaattgcc cgaaacggtt gaggcagcca atccccaagc cgcattggcg caatatttcg
     8161 ggcgcggggg ccgataagcg cgttttcttt tccatcgtgc cgtctgaaaa atgttcagac
     8221 ggcatttctt ataacctttc gtttgcagca cataaccaat cagtctttcc tattccgttc
     8281 cgaaatgcat accatgcgcc cgtacccaat atttgcgaac aaggaaagaa aatggcacgt
     8341 ttaaccgtac acaccctcga aaccgccccc gaagccgcca aagcgcgcgt cgaggcggta
     8401 cttcaaaaca acggctttat ccccaacctt atcggcgtat tatcaaacgc ccccgaagcc
     8461 ttggcgtttt accaagaagt cggcaagctc aacgccgcca acagcctgac cgccggcgaa
     8521 gtcgaagtaa tccagattat tgccgcccgc accaaccaat gcggcttctg cgtggcaggg
     8581 cacaccaaac tcgcaaccct gaaaaaactc ctttccgaac aatccgtcaa agccgcgcgc
     8641 gctttggcgg caggcgaatt tgacgatgct aaactcggcg cgctcgccgc ctttacccaa
     8701 gccgtaatgg caaaaaaagg cgcggtatcc gacgaggaac tcaaagcatt ttttgatgcg
     8761 ggctacaacc agcagcaggc agtcgaagtc gtgatgggcg tagccttggc aactttgtgc
     8821 aactacgtca acaacctcgc ccaaaccgaa atcaaccccg aattgcaggc ttacgcctga
     8881 tacggcaaac cgcccgaata tcgggcggtt tttcaacccc cctcaaacaa aaacaagccg
     8941 ccaaacggca gggaagcagc cgcgcatcag gcgtgcgccc gatttccccg caaaccttaa
     9001 ggaacaaaga tgaacgccca aaccctgatt gccaacgttg ccgaactcgt caaaaccaag
     9061 ctcaaaccca tagtggacga catcgaccgc aaaggatact accccgaagc atttatgcgc
     9121 gaactcggcg caatcggcgg attcggcgca gtcggcacag aagccgaagg cggcaacggc
     9181 ttgggtttgg caacgcaaat cgccgtattg cgcgaaatcg gcaaagaatg cggcgcaacc
     9241 tccttcagcg cgtggtgtca ggcggcttgc gcgtggtatc tgcatcaaac gcccaaccaa
     9301 gccgtcaaag acaaatacct cgccgacatc ctgcaaggca aagtattggc aggcaccgga
     9361 atgtccaata ccgtcaaaca ccttgccggc attgaaaaac acaaccttca agccgagcgc
     9421 gtggagggcg gttatacagt caacggcgcg ctgccgtggg tatccaacat cggcgaagag
     9481 catatttggg caaataccgc ccaaatcggc ggcggctacg tcatgtttat caccggcgga
     9541 caatgggaag gcgtaagcct gcaaaactgc cccgaatttt gcgccctcga aggcacgcgc
     9601 accttcagcc tgaacttcaa agacgtattt atccccgacg aagacatcat cgccgcgccc
     9661 gaacagtttg ccgactacat ccaaagcatc aaagcaggct ttatcctcct gcaaatcggc
     9721 atcggcgcgg gcgtgataga cggcagcctc ggcatcatcc gcctcgccaa catcgtcaac
     9781 gccgaagtca acagctatct cgacgacggc tacgacagcc tcaaagcaag gctggacggc
     9841 gcgtgggcag aaaccgaacg gctcgccgga ttggcttgga gcggcacgcc cgacaacctt
     9901 gctaccctca agttgcgcga agccgccgcc gtactggcac ttgccgccgc ccaatccgcc
     9961 gccctgcatt ccggcgcaaa aggctacctg atgcgcagtc ccgcccaaag gcgcgtccgc
    10021 gaagcaatgt ttgtcgccat cgtaaccccc gcgatcaaac acctccgaaa agaaatagcg
    10081 gcaatcgaag ccgcaaaata aacaggcgga aaaaaccgaa acccccaccg cgtcattccc
    10141 gcgaaagcgg gaatccagcc cccaacgcaa caggaatcca tcggaaaaaa cagaaacccc
    10201 caaaccgtca ttcccgcaaa agcgggaatc cagcccccta acgcaacagg aatccatcga
    10261 aaaaaccgaa acctccaaac cgtcattccc acggaagtgg gaatccagta accgaaaaac
    10321 cacaggaatc tatcgaaaaa aaccccaccc cccaaaaaaa ccgggcggat acctcaccat
    10381 cctcccaaac cctgatttat agcggatgaa caaaaatcag ggcaaggcgg cgaa
//
LOCUS       DAWFAN010000064        10332 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWFAN010000064 DAWFAN010000000
VERSION     DAWFAN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.064"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            292..966
                     /locus_tag="WGO65_001125"
     CDS             292..966
                     /locus_tag="WGO65_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0327422.1"
                     /translation="MQADLAYAAERITHDYPQATGANNTSTVSDYFRNIRAHSIHPRV
                     SVGYDFGDWRIAADYASYRKWKESNFSTKKVTKEINDNYQETKTEHQGNGSFHASSSL
                     GLSAIYDFKLNDKFKPYIGARVAYGHVKHQVHSVETKTTTVTPKPKNGSPQGGAVPSR
                     EPSKPPYHESHSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSL
                     GVRYRF"
     gene            complement(940..1149)
                     /locus_tag="WGO65_001126"
     CDS             complement(940..1149)
                     /locus_tag="WGO65_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327423.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKCA
                     GKGLMPSEGSDGIGIGESEAVAHAQ"
     gene            complement(1167..1724)
                     /locus_tag="WGO65_001127"
     CDS             complement(1167..1724)
                     /locus_tag="WGO65_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0327424.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1800..3434)
                     /locus_tag="WGO65_001128"
     CDS             complement(1800..3434)
                     /locus_tag="WGO65_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ0327425.1"
                     /translation="MIKPNLRPKLGSSVLIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGDIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3839..4945)
                     /gene="serC"
                     /locus_tag="WGO65_001129"
     CDS             complement(3839..4945)
                     /gene="serC"
                     /locus_tag="WGO65_001129"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ0327426.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            5310..5741
                     /gene="rimP"
                     /locus_tag="WGO65_001130"
     CDS             5310..5741
                     /gene="rimP"
                     /locus_tag="WGO65_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ0327427.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            5770..7272
                     /gene="nusA"
                     /locus_tag="WGO65_001131"
     CDS             5770..7272
                     /gene="nusA"
                     /locus_tag="WGO65_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ0327428.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            7285..10173
                     /gene="infB"
                     /locus_tag="WGO65_001132"
     CDS             7285..10173
                     /gene="infB"
                     /locus_tag="WGO65_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ0327429.1"
                     /translation="MNNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAATRAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAGKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRVGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKT
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPI
                     VVAVNKIDKEAANPERIRQELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELAAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQLTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGE
                     LSSLKRYKDDVKEVRMGFECGLMIKGFNEIMEGDQLECFDIVEVARTL"
BASE COUNT         2758 a         2981 c         2583 g         2010 t
ORIGIN      
        1 agtacggaac cgattcactc ggtgcttcag caccttagag aatcgttctc ttttttgttc
       61 atccgctata ttgtgttgaa acatcgcccc aaacctgata tagtccgctc ctgcaacatc
      121 attgaaaatc gttcttttta atcagttaaa accgaatacg gagtcgaaaa tgaatccagc
      181 ccccaaaaaa ccttctcttc tcttctcttc tcttctcttc tcttctcttc tcttctcttc
      241 tcttccgcag cgcaggcggc aagtgaagac ggcagccgca gcccgtatta tgtgcaggcg
      301 gatttagcct acgccgccga acgcattacc cacgattatc cgcaagcaac cggtgcaaac
      361 aacacaagca cagtaagcga ttatttcaga aacatccgtg cgcattccat ccacccccgg
      421 gtgtcggtcg gctacgactt cggcgactgg aggatagcgg cagattatgc cagttacaga
      481 aaatggaaag aaagtaattt ttctactaaa aaagttacta aagagataaa cgacaactac
      541 caagaaacca aaacagaaca tcaaggaaac ggcagcttcc acgcctcttc ttctctcggc
      601 ttatccgcca tttacgattt caaactcaac gataaattca aaccctatat cggtgcgcgc
      661 gtcgcctacg gacacgttaa acatcaggtt cattcggtgg aaacaaaaac cacgactgtt
      721 acccctaaac caaagaacgg ctctccacag ggaggagctg tcccatcaag agagcccagc
      781 aaacctccct atcacgaaag ccacagcatc agcagcttgg gtcttggtgt catcgccggt
      841 gtcggtttcg acatcacgcc caagctgacc ttggacaccg gataccgcta ccacaactgg
      901 ggacgcttgg aaaacacccg cttcaaaacc cacgaagtct cattgggcgt gcgctaccgc
      961 ttctgattcc ccgataccga tgccgtctga accttcagac ggcatgagac ctttgcctgc
     1021 gcacttggtg cgctggtcgc ctccgaacat ggcgcgacac ccgacatttc cgccgaacgc
     1081 atcgggcgtt tcatgaatcc ggtttaaaac gcatggaaaa atgccgtctg aaagcctttc
     1141 agacggcatt gtgcttgaga ttccgtttac caatgactga caaacgcttc caaatcggta
     1201 ttcttgggct tatgcacttc ctctgtcggc gtgccgacca tcatcagccc gatgatttta
     1261 tccttatccg cacatccgaa agcctcacgc aacagggggc tgttgaccca catccccgta
     1321 atccagacat tgtcgaaacc ttgcgccgtt gccgccagtt gcagcgcata cgccgcacaa
     1381 cccgccgtca gcatctgctc ccattccggt ttcggcttag gcacatcgcg gttcggcgca
     1441 aacgttaccc cgataaccat cggcgccata ttgcccactt tttccgcctt tttcatcgca
     1501 tcgtcgccga aattcaattc ggcaaccgtt tgcttcaaca catcgcgaaa acgttgcaat
     1561 cctacctcgc cttgaatcac ggtaaaacgg aaggggcgca tattgccgtg atcgggaact
     1621 tgggttgccg cctgaaatat ttgttccaac tccgccgcat cgggggcggg gtgcttcagc
     1681 tttttggaag atcggcggtt cgtcaataat tttaaagcat ccatatcgtt atactccggt
     1741 catcggtcgg gtgttcggac acatatgccg tccgaaggct tcagacggca tatccggcat
     1801 cagcgcggac ggcggcaggc tgccaatata tccatttcct tccgataggt ttggctgttg
     1861 gaaatgtcca tcagccccaa taccgtgctg aaatagtggt cgtgcgaata ttcgttttcc
     1921 gccgcttttt gtttgaggca ttggaaatct atgtccccgt gttggcggaa ggctttggaa
     1981 aaccacataa ccatcgggat atgcgtctgc ccagaaggcg cgatggcgta aggcgcggcg
     2041 tgcaggtaca tcccgttttc gcccaaactt tcgccgtggt cggaaacata atgcaccacg
     2101 ctttccaaat cgtcgcggtt ttcaagtttg cggataacct tgtcgataaa ctggtccaca
     2161 tacaaaaccg tattgtcgta agtgttgacc agcgtggcgc gggtgcattt gttgatttcg
     2221 ttggtgtcgc aggtcggcgt gaatttgcgt tcggcttcgg tatagcgttc gtaatacgtc
     2281 ggcccgtggc tgccgatggt atgcaggatt aaaaccgcgt ctttatcgtt tttgttgagg
     2341 acttcgtcga acttagtcag caggatattg tcgagacact cgccgttgcg gcagtattcg
     2401 ggcaggttga gcgaggtaac gtcggtattc ggcactttgc cgcacacgcc cttgcagccg
     2461 gaatcgtttt ccaaccaggt aacttccacg ccggcgcgct gcacgatgtc cagcaggttg
     2521 tcttggtgtt cggctttgat ttcgtcataa tccgtgcggt cgaaggttga gaacatacac
     2581 ggcagggagt gcgcggtcga tgtgccgcag cttctgacct gcgggaaatt gacaatttca
     2641 tcgccgcgcg cggcaagcag cggcgtagtt tggcggctgt aaccgttcaa accccagttg
     2701 gcggcacgcg tggtctcgcc cacgaccaaa accacgaaac ggcgcaggct gccggccggc
     2761 cggttttgca cgaccgccat atccaattgc gtataaggaa tattggaacg cttccaatct
     2821 ttgtatttcg acacgcccgc gccgatgaaa ttagacggca caatcagatg ggttactgat
     2881 ttattgttgc ggaaaaacga ggcgtaatcc tgatattgca acattgcgat gcccaacgcg
     2941 cacaaaaagg aaacggcggc aagcacaagg cgcgtcaaaa gctccttata ccaaacgcgg
     3001 tatttaacct tgacggcgat atacgccagc gcgggcaata cgcccaaaca tacaatccac
     3061 agcacatagc ccggcgtaat caggcgcgcg ctttcggcag ccgtagtttg caagacatta
     3121 ttcaacatcg acttgttgaa atagatattg aaaaatattt cttggtaaga caccgccgca
     3181 ctgataacca atatcaatgg aatcaatacc ttatgcacga aaggcagggc aatgacgtga
     3241 aaaacgaaat tacttaaaaa aaacagcacc accggcatcg tatagaggaa gatatccgcc
     3301 ccggtgccgt taaaaggatg aagctcgaca actttggcaa aaaaggcgta attcaatacc
     3361 agcgaggaat acagggaaag gaaggcaatc agcacggaag agccgagctt cggcctcagg
     3421 ttcggtttta tcatttggaa tgtgtcggat aagggttgga aaaggcatcc ggcatttgga
     3481 atccggatta ttgaaaaaga ttcttaatta taaggcaacg gagcaaagca gggcaagaaa
     3541 acggcggcgg cgcgaggttt ccgcccgcca ttcaaacgtc cggcagacat aaaaacatcg
     3601 taagcaagat tcgaaccggt ctgcaaccgc ccctgccaga aaaacgggca aagcatttca
     3661 tattggaaaa acccggccgc gccgccgacg ggacagtccg gcacaaacag catcacgccc
     3721 ggattgaaaa ggacggatag ccgccggcag cacagtttta ccccgcatcc caaaacgccg
     3781 ccggagaacg ccgaaacagc cgatgccgtc tgaagccgct tcagacaaca tcgggacatc
     3841 aaccgtaacg ccgttggaaa tcgcgcataa aatctgccaa agcccgcacg ccttcgagcg
     3901 gcatcgcatt gtagatgctg gcgcgcatcc cgccgacaga cttatagccc ttcaacaggc
     3961 acaagccctg caattcggct tccagcacaa aacggcggtc aagctcctca tcccccgttt
     4021 ggaacacgac attcatttta gaacgcgcat tcggacggat acggttgata taaaaaccgc
     4081 cactgccgtc tatcgtctca tacaaggttt gcgccttcag ccgattgacc gcttcaattt
     4141 ttttcacacc gccctgcgcc tgcagccagc ggaacaccag ccccgacata taaatcgcgt
     4201 aagttgacgg cgtgttgtac ataccgtcgc ggttgatgtg cgaacggtag ttgaacacat
     4261 cgggaatatc gttcggacaa cgctcgagca aatcctcacg cacaatcacc accgtaactc
     4321 ctgccggccc gatgtttttc tgtgcgcctg cgtaaatcag tccgtagtcg gcaacatcaa
     4381 actcgcgcga caaaatctcg ctggacatat cgcacaccag cggcggcatg ccttctgaaa
     4441 ggcgcggcac ttcacggtat tgcagcccgt tgaccgtttc attgacggca aaatggacaa
     4501 acgccgaatc gggtgcaaca tcccacgttt ccacaggcgg caggtcgaga tagtcgaact
     4561 gctcgccgcc atgcgccgcc aaacggattt ccgtatcggt caaacggctc atctgttcat
     4621 aagcgatacg gctccagttg cccgttacca ccgcgtcggc agtgcggaaa ccgtgtgcca
     4681 gattcatggc tgccatatta aattgggttg ttgctccgcc ctgcagaaac aatatcttat
     4741 agttgtcagg cactttcaaa agctgcctca aatcctgttc cgcatgatgc aggatgctca
     4801 aaaacatttc cgaacggtgg ctcatcgcca tcacaggaaa acccgtaccg ttgtagtcca
     4861 acatttcctg ccgcgccgtt tccaacacgg cttcgggcaa tacggcaggg ccggcggaaa
     4921 aattgtaaat cggataaaga gacatgatgc agccctgatt ctgaacaata acccgcctga
     4981 ttttaggctt gcagcaggct ttgtgcaagg atgggaaata cctgtcctcc gcccggcttc
     5041 atgccgcccg aacacggaaa ataatatcaa tatattgatt tacaaacata aaaatcatgt
     5101 acgcgacaaa tagatacatt tgttttgtca acaatattca cgatttccca ttacaaacct
     5161 cccttacacc cgcttttttc cgtcccaaaa acacaaaata aatcaacact ttcatttctc
     5221 cgcaaaagcg gttataatca cgccgatttt tcaactttga cgaaaaatgg ccacggggcc
     5281 atttttttat tatgtacata gggagcagca tggatatcca aaccatcctc gaaaaaaccc
     5341 tgcccggcct gggctacgaa ctggtcgatt ttgaactgac cgcgcaagga acattgcgcg
     5401 tgttcatcga caaagaaggc ggcattaccg tcgaagactg cgcaaccgtc agcaaccact
     5461 tgagccgcgt cttcatggtt gaagacatcg actacaaaaa cctggaaatt tccagccccg
     5521 gactcgaccg ccccttgaaa aaagccgccg acttcgtgcg ctttgccggt cagaatgcca
     5581 aaatcaaaac ccgcctgccg atagacggtc agaaaaactt tatcggtaaa atcgaaggct
     5641 gcgaaaacga taccgttacc gtatccttcg acggcaaaac cgtacaaatc gaattgggca
     5701 acatcgacaa agcccgtctg cgccccgaat tcaaattcta aaacacaaca ataattggag
     5761 atgttcaaaa tgagtcgtga aatgttacag ctggcagaag cactggcaag cgaaaaaaac
     5821 gttgatgccg aagtcgtctt ccaagcactg gaattcgccc tgtctaccgc cgccaagaaa
     5881 aaggcagacc gcgaacacat ggacgtgcgc gtccaaatca accgcgatac cggcgaatac
     5941 caaaccttcc gccgctggct gattgtcgcc gatgaagact atacctatcc cgatgtcgaa
     6001 aaaaccatcg aggaaatcca agaggaaatt cccggcaccg ccatccaaat cggcgaatac
     6061 tatgaagagc agctgcctaa cgaaggcttc ggccgccaag ccgcacaaac cgccaaacaa
     6121 atcatcctgc aacgcatccg cgatgcggag cgcgagcaaa atctgaacga gtttctcgcc
     6181 gtcaaagaag acatcgtgtc cggtacggtc aaacgcgtcg aacgccacgg catcatcgtc
     6241 gaagtcgttg ccggcaaact ggacgcgctg attccgcgcg accaaatgat tccgcgcgaa
     6301 aacttccgca gcggcgaccg catccgcgcc ctcttcctgc gcgtcgaaga aatcggcaac
     6361 accggccgca aacaagtcat cctgagccgt acttccggcg atttcctcgt caaactgtac
     6421 gccaatgaag tacctgaaat tgcagacggc atgcttgaaa tccgcgctgt cgcccgcgac
     6481 ccgggccagc gtgccaaagt cgccgtcaaa gccaacgacc agcgcatcga tccgcaaggc
     6541 acctgtatcg gcgttcgcgg ttcgcgtgtc aatgccgtca gcaacgaatt gtccggcgag
     6601 cgcatcgacg ttgtcctttg gtcgcccgag cctgcccaat tcgtcatgag cgcgctctca
     6661 cccgccgaag tcagccgcat cgtcatcgac gaagacaaac acgccgtcga tgtcatcgtt
     6721 gccgaagacc agctcgcgct cgccatcggg cgcggcggtc aaaacgtgcg ccttgcttcc
     6781 gacctgactg gctggcagct caacatcatg acttccgccg aggcagacga acgcaatgcg
     6841 gcagaagatg ccgccatccg ccgcctgttt atggatcact tgaacgtgga cgaagaaacc
     6901 gccgacgtac tggttcagga aggttttgca accttggaag aagtcgccta tgttcctgcc
     6961 gccgaactgc ttgccattga aggatttgac gaagaaatcg tcgatatgct ccgcaaccgc
     7021 gcccgcgatg ccatcctgac catggcgatt gccgccgaag aaaaactggg cgaagtgtcc
     7081 gacgatatgc gcaacctcga aggcatagat gccgatatgc tccgcagcct tgccgaagca
     7141 ggcattacta cccgcgacga cttggcagag cttgctgtgg acgaactgat tgaaatcacc
     7201 ggtgtaaacg aagaaaccgc aaaagccgtc atcctgaccg cacgcgaaca ctggtttacc
     7261 gaagacaaat aaaagggggt acagatgaat aacacaaccg tagaacaatt tgccgccgaa
     7321 ctgaaacgcc ccgtcgaaga cctgttgaaa cagttgaaag aagccggcgt cagcaaaaac
     7381 agcggcagcg attccctgac gctggacgac aaacagcttc tgaacgccta cctgaccaag
     7441 aaaaacggca gcaacggcgg caccatcagc atccgccgca ccaaaaccga agtcagcacc
     7501 gttgacggcg taaaagtcga aacacgcaaa cgcggacgca ctgtcaacat tccttctgcc
     7561 gaagaattgg cagcacaggt aaaagccgcc caaacccaag ccgcacctgt ccggccggag
     7621 cagacggcag aagacgcggc aaaagcccga gccgaagctg ccacacgtgc agaagcccgt
     7681 gccaaggcag aagcggaagc ggcaaaactg aaagcggcaa aagcaggcaa caaagccaaa
     7741 cctgccgcgc agaaacccac cgaagcaaaa gccgaaaccg cacccgttgc ggcggaaacc
     7801 aaacccgccg aagaaagcaa agcgggaaaa gcccaagccg acaaaatgcc gtctaaaaaa
     7861 cccgccgagc ccaaagaaaa agccgccaag ccgaaacacg agcgaaacgg caaaggcaaa
     7921 gatgccaaaa aaccggcgaa acctgccgca cctgccgtgc cgcaacccgt ggtcagcgcg
     7981 gaagaacagg cgcaacgcga tgaagaagca cgccgtgccg ccgcacttcg cgcccaccag
     8041 gaagccctgt tgaaagagaa acaggaacgc caggcacgcc gcgaagccat gaaacaacag
     8101 gcagaacaac aggcaaaagc cgcacaggaa gccaaaaccg gcagacagcg tcccgccaaa
     8161 cctgccgaga aaccgcaggc agccgcgccg gccgtcgaaa acaaacctgt caatccggca
     8221 aaagcgaaaa aagaagaccg ccgcaaccgc gatgacgaag gtcaaggccg caacgccaaa
     8281 ggcaaaggcg gcaaaggtgg tcgcgaccgc aacaatgcgc gcaatggcga cgacgagcgc
     8341 gtacgcgtcg gcaaaaaagg caaaaaactc aaactcgagc cgaaccaaca cgccttccaa
     8401 gcgccgaccg aacctgtcgt acacgaggtt ttggttcccg aaaccattac cgttgccgat
     8461 ttggcacaca aaatggcggt caaaggcgtg gaagtggtca aagccctgat gaagatgggc
     8521 atgatggtaa ccatcaacca atccatcgac caagacaccg ccctgattgt ggtggaagaa
     8581 ctcggacata tcggcaaacc tgccgcagcg gacgatccgg aagcattctt ggacgagggc
     8641 gcggaagcgg tggaagccga agcattgccg cgtccgccgg tggttaccgt gatggggcac
     8701 gtcgaccacg gtaaaacctc gctgctggac tacatccgcc gcgccaaagt ggtgcagggc
     8761 gaagcaggcg gcattacgca gcacatcggc gcgtaccacg tcaaaacgcc gcgcggcgtg
     8821 attaccttct tggatacccc gggccacgaa gcgtttaccg ccatgcgcgc acgcggcgcg
     8881 aaagcgaccg acatcgtgat tctcgtggtt gccgcagacg acggcgtgat gccgcaaacc
     8941 atcgaagcga ttgcccacgc caaagcggca ggcgtaccga ttgtggttgc cgtcaataaa
     9001 atcgacaaag aagccgccaa ccccgaacgc atccgtcagg aactgaccca acacgaagtc
     9061 atccccgacg attggggcgg cacggttcaa ttcatcgatg tttccgccaa aaaagggaca
     9121 aacatcgacg cgctgctcga agccgtcttg ctcgaagccg aagttttaga actggccgcc
     9181 ccggtcgatg cgcccgccaa aggcatcatc gtcgaggctc gtttggacaa agggcgcggc
     9241 gcggttgcca cattgctggt tcaaagcggt acgctgaaaa aaggcgacat gctgctggcc
     9301 ggtacggcat tcggcaaaat ccgcgcgatg gtcgatgaaa acggcaaatc cattaccgaa
     9361 gccggcccgt ccatcccggt cgaaatcctc ggcttgtccg acgtacccaa tgcgggcgaa
     9421 gacgcgatgg tattggcgga cgagaaaaaa gcgcgcgaaa tcgccctctt ccgccaaggc
     9481 aaataccgcg acgtgcgcct tgccaaacag caggcggcga agctggaaaa tatgttcaac
     9541 aatatgggcg aaacccaggc ccaatctttg tcggtcatca tcaaggcaga cgttcagggc
     9601 tcttacgagg ctttggcggg cagcctgaaa aaactgtcca ccgacgaggt gaaagtgaac
     9661 gtgttgcaca gcggcgtggg cggcattacc gaatcggatg tcaaccttgc catcgcttcg
     9721 ggcgcgttca ttatcggctt taacgtgcgt gcagatgcct cttcgcgcaa acttgccgaa
     9781 aatgaaaacg tggaaatccg ctactacaac atcatctacg atgccatcga cgacgtgaaa
     9841 gcggcgatga gcggtatgct ttctcccgaa gagaaagaac agcttaccgg cacggtcgaa
     9901 atccgtcagg tcatcagcgt ttccaaagtc ggcaacattg caggctgtat ggttaccgac
     9961 ggcgtggtca aacgcgattc ccatatccgc ctcatccgca acaacgtggt catccatacc
    10021 ggcgagctgt cgtctttgaa acgctacaaa gacgatgtta aagaagtccg tatgggcttc
    10081 gagtgcggcc tgatgattaa aggcttcaat gaaattatgg aaggcgacca actggaatgc
    10141 ttcgacatcg tcgaagttgc acgcaccctg tgattttgat gcggaaaaat gccgtctgaa
    10201 gcgttcagac ggcatacgaa acgggttctg tatcatacag aacccgtttt ttgtcgcaaa
    10261 tcggcttcag acagccctct tgccttatcc cgatttgaat ctgacttgcc atacaaacag
    10321 gcttcagacg gc
//
LOCUS       DAWFAN010000065        10284 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWFAN010000065 DAWFAN010000000
VERSION     DAWFAN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.065"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(92..1234)
                     /locus_tag="WGO65_001133"
     CDS             complement(92..1234)
                     /locus_tag="WGO65_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ0327430.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAAGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQVNPAFAPISGSDNDRRIVQTALDNARSA
                     EVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1283..1705)
                     /locus_tag="WGO65_001134"
     CDS             complement(1283..1705)
                     /locus_tag="WGO65_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327431.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1698..2507)
                     /locus_tag="WGO65_001135"
     CDS             complement(1698..2507)
                     /locus_tag="WGO65_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0327432.1"
                     /translation="MTRDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2581..2958)
                     /locus_tag="WGO65_001136"
     CDS             complement(2581..2958)
                     /locus_tag="WGO65_001136"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ0327433.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYAPALSEWLEEQG
                     IRHVNLSMADEGDTVLAFAVAEK"
     gene            complement(3158..3925)
                     /locus_tag="WGO65_001137"
     CDS             complement(3158..3925)
                     /locus_tag="WGO65_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0327434.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3935..4714)
                     /locus_tag="WGO65_001138"
     CDS             complement(3935..4714)
                     /locus_tag="WGO65_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ0327435.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQSIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFLDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPA
                     SFDKKQGVRWGNILSVNTQMIRFRSDCIYKGIEENRNKVSKEMVLALDYLVNVIKNAS
                     DIQEFSAQDDDLIIIDNVNGLHAGTDYTDKNRHYIRARITV"
     gene            complement(4713..4820)
                     /locus_tag="WGO65_001139"
                     /pseudo
     CDS             complement(4713..4820)
                     /locus_tag="WGO65_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4866..5594)
                     /gene="pdxJ"
                     /locus_tag="WGO65_001140"
     CDS             complement(4866..5594)
                     /gene="pdxJ"
                     /locus_tag="WGO65_001140"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ0327436.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAEFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(5647..6390)
                     /gene="recO"
                     /locus_tag="WGO65_001141"
     CDS             complement(5647..6390)
                     /gene="recO"
                     /locus_tag="WGO65_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ0327437.1"
                     /translation="MSEHRINHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWVGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(6424..7551)
                     /gene="pheA"
                     /locus_tag="WGO65_001142"
     CDS             complement(6424..7551)
                     /gene="pheA"
                     /locus_tag="WGO65_001142"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ0327438.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASSSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(7590..8822)
                     /locus_tag="WGO65_001143"
     CDS             complement(7590..8822)
                     /locus_tag="WGO65_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ0327439.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            complement(9342..10196)
                     /locus_tag="WGO65_001144"
     CDS             complement(9342..10196)
                     /locus_tag="WGO65_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0327440.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT         2472 a         2781 c         2660 g         2371 t
ORIGIN      
        1 atcctgattt ttgttaatcc actataatat cggccggata ttaaaaaaag ccgtctgaac
       61 atttttcaga cggcattttt gtacactttc ctcaacggtt cgatccctgc accatatcaa
      121 tcaggaacag gcggcaatcg aggttgccgt tataaagcgg aattttccgc ttgggcgaca
      181 ggcgcatgaa tttgggcatt tccctgtcgc cggtaaacat accgacccgc cagccggcat
      241 agtgtttttt caaccacgtc cccaactgcg gataaagtgc ctgcaaagcg cggacttcct
      301 caaggcgcac accgtagggc ggattggaca ccatgatgcc gttttcgccg ttcggacgga
      361 cggattgcgc gtcggcaacg ctgaaggaaa cgatgtcatc cacttcggca ctgcgtgcgt
      421 tgtccaatgc cgtctgaacg atgcggcggt cgttgtcgct gcccgaaatc ggtgcaaaag
      481 cggggttgac ctgcgcttcg gcgcggcggc gcaaatccgc ccacagcgtt ttatcgaaat
      541 tttgcagttt ttcaaaaccg aagcggcgca tcatacccgg tgcgcggccg gcggcaatcc
      601 aagcggcttc gatagcaatc gtgccgctgc cgcaaaacgg gtcttgaaac ggctgcgtgc
      661 cgtcgtagcc tgccgagagc agcaatccgg cggcaaggtt ttcgcgcagc ggggcttcgc
      721 cggtatcgag acggtagccg cgtttgaaca gggcttcgcc cgaagtgtcg atgaagattt
      781 cgacattgcg ttcgtccaaa aaggcgtgga tgcgaacatc gggcgcggct ttgtccacgc
      841 tcggacgtgc gtcgtaaatg tcgcggaaag cgtcgcagac ggcatctttg acggtcaaac
      901 caacaaattg gatgctctta acgttggcac gctttgcctc gactttgact ttgaacgtct
      961 gctgtaaagt aaaccaatta aaccagttga tatttttggc gagtttgtag atgtcgcgct
     1021 cattgcggta tgtccctttg gtcaggcgca gcaggatacg gctggcagta cgcgaatgca
     1081 ggttggcggc gtaaacctgt tccaatccgc cccggcagga aacgccgccg tcaaacactt
     1141 gtacatcggt acagccgagg ctttcgagtt cttgagataa aacggtctcc aagccgcgcg
     1201 ggcaggtggc gaaaagtgta tataaagtat tcataacgtg tcctttccgg acggatgttc
     1261 cggcaatggc ggattgaaag gtttattcgg cggtaaatgc cgtctgaaat gtccgactgc
     1321 cgattgtaat gtccgccgta aaatcgcgcc tgccttcgac acagatgggc aggcggatgc
     1381 gtactgcctg ccaagtcccc gacggttgcc gctcgaacat atagcggttg aaacccatat
     1441 ccatattttt catactgaag ctgatgctga cctgttccgt gccggcgggc gcgtgttcga
     1501 tataaatatc aaacggtttt ttggttgaaa cggcggcggc gcggacgcgg cttccgtccg
     1561 gcagcgtgca accctcggtc aaatcgcatt gcgccgccac agcttgcggc tgctgcgcct
     1621 gccaccattg caacaaaacg agcttgacgg cggcaaaggc aatcagcagc agtgcggcaa
     1681 gcagtaattt acggttttta ttcatgattc ttccctattg tttcatccct taccaccacg
     1741 tgcgccccca atccgagcca tagttttcca taacgtgcaa ccgtctgtcc gtttgctgca
     1801 agtacgagcg gcacggatac gccctgccgc agggcttctg ccgcctcttg tgcctgaaca
     1861 tcatcgcaca cgcgccaaac gaccgcatcc gcgccctttt cctgcgcccg ccgccattgt
     1921 tcgcgggtct gcaccaccat ccacacgctg tcggcgtgtt cgggtctgtc ctgccattgc
     1981 gccgcctcca tcaaaacgtt cgcaccgctt ccctctgccg aacccaaagg caggacgcgg
     2041 tacgcgccca tactgttttc tccgtgcaaa cccgttttca ggctgccgta caaacggcgc
     2101 gggacggaca gcgaacgcaa aagcgcgccg ttggcgggca gcatgggggc aacggtaaaa
     2161 tcacccgcct tctgccaaga ccattcctgc ccttcgcggg attgcggttt gcccgtccat
     2221 tggtcggggt tgacccatag gaatttcagg cagacgcggg cgtgttcgta ggaatggatt
     2281 ttggtcagcc aaggcgtggc ggcgaggatg cggatgccga gttcttcttc aaactcgcgt
     2341 tgcaaggctt ggaagtcggt ttcgcccgct tcgaccttgc cgccggcaaa ttcccaatat
     2401 ccggcatagg gtttgccttc ggggcgcgag ctgagcaggt agttgccgtc tgaatcgagc
     2461 aggatgccgg caacgacgcg gataaggggt cgggtgtctc gggtcatagg aaagatggga
     2521 aaagtttttc cgaacattag aaaagggcgg aacggtcaat ccggcccggc ggctgccgcc
     2581 ttatttttcg gcaacggcaa acgccaatac cgtatcgcct tcatccgcca tactcaaatt
     2641 aacgtggcgt atgccttgtt cctccagcca ttcggacagg gcgggggcgt agaaaaattc
     2701 cggtttgccc aacgcgtcgt gcccgatgcc gatgttgcgg aaggaaaccg caccgcgtat
     2761 gcccgtgccg acggctttgg cgaaggcttc tttggcggca aagcgtttgg caaggtagtt
     2821 gacgggtttg cccgcttgcg gaaattcaag cagctcttcc ggagtgagga tgcgcccggc
     2881 aaacgcctgt ccgaattttt tgtttaagcg gatgatgcgc ttgagggaaa caatgtctgt
     2941 gccgatgccg taaatcatat ttgcgctcct tcgcccttgg ttgcgggtag tgatgaatgt
     3001 gatgatgaat gccgtcaatt tcttggggcg ttaaaatggc gtgaatatcc acttttgggg
     3061 ttttgtcggt aacaattttc actgtaacct tttgcatttg aaattctaaa acatcgtgcc
     3121 aattatttaa taaggagtta aggcatttaa taatgaatta aagtttgatt aaacccgaat
     3181 gcccgatttt cagagccaat accaataaaa caataaaaat taacatttta aaaaaatttg
     3241 ggctaatctt agtccttaac cgaattccaa catacaatcc aataacagac aatacggaca
     3301 gtaaaaatat taaaccgtat tcactcttat ttaataacca atactggtct cttagcatat
     3361 atatttgaac aattttcgcc aaaagatagc atagattgct tgattttacg atacgatttt
     3421 tattttctgt ttcgctaagc aaaaatatta acaatatggg agacatggca ttggttgaac
     3481 cgccgatgat gcctgccaaa aacccaaaaa gaaccatatt cttattattg gcaactactt
     3541 gaatattttt tgcttttgca catacattta aaataccatt gacagaataa tacaatgtaa
     3601 tgattgccat cagtaaaagc agccaagaca ctggaagtat caaaagcaac ttcaccccca
     3661 aaatgctgcc aacgacgctg ccgatagcaa gcaatttata ggtttttaaa taataaacaa
     3721 tctcttgcca aaaacccttt ttgttattgc tgcatagaac caacaagctc attaacaggc
     3781 ttggtaatgc caccaaggca acaaccttag acaatggcat gttaaaagcc aatgcggttg
     3841 taccgagcat cggaaatccc atgcctgtaa ttccgtgcag tattgcggca gcaacaaaaa
     3901 cgatagattg cattatttct tgcataaccg tcctttatac agtaattctt gctctaatat
     3961 aatgcctgtt tttatccgta taatcagttc cggcatgcaa accattgaca ttgtcaataa
     4021 taatcaaatc atcatcttgt gcagaaaatt cttgaatatc actcgcattt tttataacat
     4081 ttacaagata atcaagtgca agtaccattt cctttgatac tttatttcta ttttcttcaa
     4141 tacctttata gatacaatca cttctaaatc taatcatttg agtattgacc gataagatat
     4201 taccccatct cacaccttgt tttttatcaa atgatgctgg tgttttaaat ggatataaat
     4261 tgcccgttaa tgtttttaag tgttttttac cggtttctgt tttagataac tgattgacaa
     4321 tatctgatga acttaaaaat agggaattac ctccatcatt ggctgatttt actacataca
     4381 taaccaaata actttccggg ttttcactaa aagatgaatc actatgaagt ggacattctc
     4441 caacatcttc cgagaaagtt atatcattat ttattacata gtcatcgccg cgatatttaa
     4501 tatccaaaaa tatagattta aaaccatcgt ctttgtggtt tgtcagcatt cctaattgag
     4561 atagaaagtc aaataatttc ttgtttctcc ggctgtcatc aagatggttt agattaagac
     4621 cgcgaacaat acaataccca atactctgta ctgtattgtg aatttcttct atcaaactat
     4681 tattgtttgc agataaaata tacttttgat tcatatagtg aattaaattt aaaccagtac
     4741 ggcgttggct tgccttgccg tactatttgt actgtctgcg gcttcgccgc cttgtcctga
     4801 tttaaattta atccactata acatatccct gtcgtgaaat gctgtctgaa agggtttgcc
     4861 tgcccttacg gcagcagcct tgccctgaac atcgcctcct tcatctggcg cacggcttcg
     4921 ggcagtccga ggaagagggc ttgggcaatc agcgaatgcc cgatgttcag ttcgcggatg
     4981 gcgaggattt gggcgatggg ggtaacgttg tgtatggtca gtccgtgtcc ggcgttgacg
     5041 accaagccca aatcgccggc aaaatgcgcg ccgttttgca gacgctcgaa ttgcttgagt
     5101 tgttcggcgt gactgcgtgc atcggcatat gcgccggtgt gcagctcgac aacgggcgcg
     5161 ccgacatcat aggcggcttg gatttgcctg tcgtcggcat cgataaacaa agacacgcgt
     5221 atgcccgcgt cggtcaggat tttggtgaat tcggcgattt tttcctgttg cgccaatacg
     5281 tccaaaccgc cttcggtcgt gatttcctgc cgtttttcag gcacgatgca cacgtcttcc
     5341 ggcatcactt taagcgcgtt ttcgagcatt tcttccgtca acgccatttc aaggttcagg
     5401 cgcgtgcgga tggcgttttt gacggcaaac acgtccgcgt ctttgatgtg gcggcggtct
     5461 tcgcgcaggt gcatggtaat caggtcggca ccgtgcgttt cggcaaccag tgccgcctcc
     5521 acggggctgg gataagtcgt gccgcgcgcg ttgcggacgg tggcgatgtg gtcgatgttg
     5581 acacccaaaa gcataatgtt tccttttctc ttcgtttttc tgtttggcgg catggtgtgg
     5641 gcgcagctac gcggtattgc ggtcaaactg ccttatctgt tccaacacct gccgcgattt
     5701 caagccttcg ggaagcagcg tgccgatcag cagccgtgtg attttcaatg cctgttgcag
     5761 gctttcggcg gtgcggaaac tgccttcgcg caaatcgatc aggctttgcc cttcgacaat
     5821 tgcgccggcc tcgtgcgaaa gcgcgtctgc gtcctcgcat acgggcatga ccgcctcttc
     5881 gggcatcagc cggtaggttt tgtccgccaa aatatcgccg cccgtgccgt cggcgtgcaa
     5941 atcgggggca acgcccaagg cattgagcag cttccactcg aagcggcgca aatcggcaat
     6001 gtggttggct tcacgacaca ccgcctccat cacttttgcc aacgcgtcgt acagttcggg
     6061 catcggatct tcgcgcgcgg tcagtttcaa caccagttcg ttcacataca gaccgctgaa
     6121 caacgccctg ccctgcggct gccgccatcc gccgacccat tcggcacggt gaagggtttt
     6181 gagttcctga ctgccgtacc acgacgcact gacgggcacg aaaggaacca atacgccgcg
     6241 caattcgctc tgccgcttgc gcgcgcttct tgccaaaagt gccacacgcc cgtaacggcg
     6301 gctgaaggct tcgacccgca ggctgctttc gcgccagggc gatgatgcca acaggaagac
     6361 gggttcgtgg ttgatgcggt gttcggacat catgcctgaa atgccgtctg aacgctgccg
     6421 ccgctacaaa acggcggtcg ggtacgaacc gatgactttg acgaacgaag cgcgttcgcc
     6481 caagcgttcc aatgccgtct gaatctgcgc gtcccggcgg tgtccttcga tgtcgatgaa
     6541 gaacaggtat tcccacaaaa cggatttgct cggacggctc tcaaacttgg tcatggaaat
     6601 acccgactcg gtcagcggtt gcagcagcga ggcaaccgca ccggcgcggt tgggcgcgga
     6661 aacggccagc gaagtcttgt cgctgctgct tgcaccggtt tcgtgatgtc ccatcaccaa
     6721 gaagcgcgtg gtgttgttcg gttcgtcttc gatgcactcg gcaaccatat cgagtccgta
     6781 gatttccgcc gccgtgcgtc cggcgatggc ggcaaccgta ccgtcgtccg attcggcaac
     6841 cagccttgcg gcttcggcat tgctggacac ggcaatccgt tcggcgttgg gcaggtgtct
     6901 gcccaaccag tcgttgcact gcgccaacgc ctgcgcgtgg gaaaagactt tggcaatgcc
     6961 ttcggtgctg ccgttgtttt tacgcaaaag gttgtggtgg atgcgcaaaa cgacttcgcc
     7021 gcacgcctgc aacgcggtaa cggcaagcaa atccaacgtg cgcccgaccg agccttcggt
     7081 cgagttttcc acgggggcga ccagatagtc tgcctgacgg gtttcaacct gcttgaagca
     7141 gttgtctatg gtcggacacg ccatggtgtg cgcggcgtgt ccgaaatgct taatcgccgc
     7201 ctgctgggta aacgtgccct gcggccccag ataggcgatg gtcagcgggc gttcgacggc
     7261 gaggcactcg ctcatcactt cccgaaacag gcgtgctacc gattcgtcgg gcagcgggcc
     7321 tttgttcaaa tcctgaatgc ggcgcaacac ggcgacttcg cgttcggggc ggtacactgc
     7381 gcccgtgcct ttcagctcgc cgatggcgtg ggcgtgttgc gcacgttcgt tgagcaggcg
     7441 caggatttcg gcatcgatgg tgtcgatggc gttgcggtgg ggaaggagga gttcgtcgat
     7501 agtttgggac ataatttcgc ctgaacagta atttatcgtg cattctaaca tcaaatgccg
     7561 tctgaaacgg tttcagacgg cattttccgt taaaggtatt cgctctgttc gttttccttc
     7621 cacgcccggt gcgaacacag ccacagaagc gcaatgccgc aagaggtgga cgcggtcatc
     7681 gttgccgcca tcacggttgc cgaaccgtcg tgcaagaagg tcgccgccat acccaccccc
     7741 gcgccgatta aggactggaa cacgcccaac acggcgttcg cgctgccgcc ctcttctttg
     7801 aaataagaca taaagcatgc ctgcgtgttc gcgccgacca agccctgcgt gccgacggaa
     7861 aacatcacgc acgcaaccaa gagccagaac ggaggcaacc cgaaaaacag cacggcggcg
     7921 agttgggaca ggttggcggc aaattggacg acaatccccc acagcaggat gctttgcgga
     7981 tgcgcgccgg ttttgagccg ccacgcggta atgcggttga aaaacatcat cgtgatgatg
     8041 ttgagtgcaa acgcccaagc gtattgatga ggcgtaacac ggtaaagctg ctggtacacg
     8101 aaggaagatt cggtcagaaa ggcgaacatc gaaccgaagc tgaatgcctg aaaaaacaga
     8161 taacccatcg cggcacgggt tttcaatacg cgtttgaacc gcccagccac cagcccgaac
     8221 acatcccgtc cgattttgcc gccgacggca ggcttgggca ggaaatactg caccaaaccg
     8281 agcagcacca gcgaatacgc cgccagaaaa acaaaaatcg cctgccagcc acccaagccc
     8341 tgcaacagtg cgccgaccat gggtgcgacc agcggcacaa ccatcaaaat gatgccgata
     8401 agggcaaaca tctgggcggc tttgcgtccg gaataataat cgcgcaccat tgcgccgacg
     8461 atgaccacag tcatgcccgc accgaatgcc tgcacgacgc gcaggttgag gagctgttcg
     8521 gcactcgaag caaatacgat ggcggcaacg gcaaggcagt agacggccag tccggtcagc
     8581 gcgacgggtt tgcgcccttt gatgtcggac accgaaccgc cgaccacctg tccgaacgcc
     8641 gtgccgaaca taaacaaact caggctctgt tcgatgcggt ggacatccgc gttcagcgac
     8701 tgcgccattt cgggaatcgc gggcaggtag gcatcgatgg aaaacggcat cagcgtaacc
     8761 agcatcgcca tcaaaaccgc catcagtttt tcgctcattt caggataatg ggcagaaggc
     8821 ataaattctc gtccgattcg caaacaaacc gcccggcggc ggaaaacaca atacgcggaa
     8881 acggaaaatt ttaacacacc ggcacaaaca ggcggcaaca aaagccccgg caaaaccgga
     8941 cgaaccggaa cgatatagtg gattaacaaa aaccagtaca gcgttgcctt gccttagctc
     9001 aaagagaacg attctctaag gtgcttaagc acccagtgaa tcggttccgt actatctgta
     9061 ctgtctgcgg cttcgtcgtc ttgtcctgat ttttgttaat ccgctataaa atcccgtcat
     9121 tcctgcgcag gcgggaatcc ggacatccaa cgctgcagca atttgtcgga aataaccgaa
     9181 actcaaaaaa ccggatgccc gcctgcgcgg gaatgacgat gcaaagatgg tcgttatttc
     9241 ggataaatat ccgtcttttc gttccggata cccgctttcg caggaatgga gatggaaaga
     9301 tttcagccta ttcggcaatc atcaaatacc gcttcccaac tttatttttg atgcgacagc
     9361 cggccggcaa ccaaggtggc ggcgatgacg acaaacacgc ccaaggcgga cacgggcgac
     9421 aggtgttcgc ccaaaatcaa aaccgccagc agcacgccga cgacgggttc gagcgaaatc
     9481 aacagtcccg aaacgttggc aggaacacgg ctcatcccct tgttccacag ccaataggcg
     9541 taccagccgc accccaaacc caaatacagc agcgacaata ccatcccgac gctccagtcc
     9601 acggtataac tttgcgccaa agcaagcgaa aacggcaggc acatcaacga tgcggcggca
     9661 atggaaacag atgtgaatgc cggtgcgccg atgcgtgcaa tcagcctttg cgtcggacgc
     9721 atagcggcac aaaagcccgc gcccgccaac aacaccagca ggcagccgaa ccagccgact
     9781 tcgccgccct cttccgcacc gcccgccatc agcagcgcga caccggcaaa tgccgccgcg
     9841 ccgcatatcc agtggtaggc acgcgctttg tcgttgaaga aaaagtgtcc gacaaacacc
     9901 atcagcagtg gctcgagtcc gacaatgacc gatgcgctgg cggcggaagt gtatttcaac
     9961 ccgacaaact gaagcagcag ggtcagcaca tagttgacga acgacacaat cagcaacggc
    10021 ttccattcct cacgcggaat cttgccgaca tgacggcggc aggcgggcag tgcaggcagc
    10081 gcagcaatca gcaggcgcac gccgaccatc aatgcgggat cgatgccgcc atagacatat
    10141 ttggcggcaa taaacgagct gctccagata atcagggcaa ggatttggta aaacatagtg
    10201 agtgataaag tcagatggtc atacaaaagt atagcggatt aacaaaaatc aggacaaggc
    10261 gacgaagctg cagacagtac aaat
//
LOCUS       DAWFAN010000066         9797 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWFAN010000066 DAWFAN010000000
VERSION     DAWFAN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.066"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(12..1307)
                     /locus_tag="WGO65_001145"
     CDS             complement(12..1307)
                     /locus_tag="WGO65_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0327441.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1433..2320)
                     /locus_tag="WGO65_001146"
     CDS             complement(1433..2320)
                     /locus_tag="WGO65_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0327442.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2320..3285)
                     /locus_tag="WGO65_001147"
     CDS             complement(2320..3285)
                     /locus_tag="WGO65_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0327443.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNITISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3301..4425)
                     /gene="potA"
                     /locus_tag="WGO65_001148"
     CDS             complement(3301..4425)
                     /gene="potA"
                     /locus_tag="WGO65_001148"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0327444.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4620..4727)
                     /locus_tag="WGO65_001149"
                     /pseudo
     CDS             complement(4620..4727)
                     /locus_tag="WGO65_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="WGO65_001150"
     CDS             complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="WGO65_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ0327445.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5298..6233)
                     /gene="secF"
                     /locus_tag="WGO65_001151"
     CDS             complement(5298..6233)
                     /gene="secF"
                     /locus_tag="WGO65_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ0327446.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6237..8090)
                     /gene="secD"
                     /locus_tag="WGO65_001152"
     CDS             complement(6237..8090)
                     /gene="secD"
                     /locus_tag="WGO65_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ0327447.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRVGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="WGO65_001153"
     CDS             complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="WGO65_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ0327448.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8696..>9797
                     /locus_tag="WGO65_001154"
     CDS             8696..>9797
                     /locus_tag="WGO65_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ0327449.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAVAEYRLNRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRAFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2511 a         2581 c         2429 g         2276 t
ORIGIN      
        1 gcctgcgacg cttaacggct gtctttccac tgataatatt tcgagccgag gaaagaaccg
       61 agtttgcgca aaaacggttg caggataata ttcggctggc gcaaccgctc aaacggcttc
      121 agacggcatt cgtcccctaa aattgcttcg gcaaccgcca gacctgcaat gcctgttatc
      181 gccatcccgt gtccggaata accttgtgca taaaaaacat ttgcggccaa acgtccgaaa
      241 tgcgggacaa ggttggcggt aatgtcgcac tccccgcccc acgaatattc gattttgaca
      301 tcgtcaagct gcggaaaaac tttaagcata tcttggcgga caagctcggt catacgcgcg
      361 ggattgtcga taaactcgtt atccttaccg ccgaaaagca gtctgccgtc cgcgctgagg
      421 cggtaataat ccaaaatatg gcggttgtcg catactgcca tattgttacg gataagccct
      481 tttgcgcgcg cccccaaggg ttcggtcgca ataataaagg tgctaacggc aatcgccttg
      541 cgttccaaag gctggaatat cgggttcaaa cctgcatagg tattgacggc atacaccaga
      601 tttttgcatt cgacgctgcc ttcgggggtg taaaccagcc aaccgttttg ataaggttca
      661 atgcgtatca tgggagaatg ctcaaaaatc ttcgtaccag cttcggcagc ggcgcgggcg
      721 atgcccaacg tgtaattgag cggatggaga tgcccggaca agggatcgaa ctgtgcgcct
      781 tggtacatat cgctgtcaag ctgctgcttc agttcagtgt tatcccagag ttgataatga
      841 gttgcaccgt aatatttttg ggcgtgctca tgccattgtt gcaattcttc ccaatgctgc
      901 gaacggatgg caaccgtggc ataaccgcgc tgccaatcgc aatcaatggc atgtttgcgg
      961 acgcgttcgt ccaccagttc gaccgcctgc aaagactgtt gccaaaacca ttgcgcttgc
     1021 tccaaaccga cctgtttttc aatttcctcc ataccgcagg cgtaatcgct gataacctgc
     1081 ccgccactcc gtcccgacgc gccgaaaccg atacgcgcgg cttccaacac aaccgtttca
     1141 tgtccctgct ccgccaaggg caatgcagtg cacaaaccac tcaatccgcc gccgatgata
     1201 caggtatcgg ttttcagacg gcattgaagt ttcggataaa cagtatgagg attaaccgaa
     1261 ctgaaataat aagaaggcag atattcttga aaatcagggc gaatcattgt gtttgcttta
     1321 tcgggtatat tttcggacgg aatgatacag gctgtcgagc catatcgtcc aaacagaaaa
     1381 tcggttgaag aaaacaggct gacccagtca tgcggtcagc ctgccttatt aattaatttg
     1441 ctttctcggc agccaatttt tcttggcggt aggcttctgc cgcttctcgg tcacgcttgg
     1501 ttgcctgcct catcatccaa taattgacga tgatgaccaa tgttccgatg atgccgatca
     1561 ggatggttgc caaaacgttc atctgcggat caagacccag cttgattttg gagaaaatca
     1621 cctgcggcaa tgtggatgaa ccggggccgg agaggaatga ggtaatcacc aaatcatcca
     1681 aagacagggt aatgccgagc agaaagcctg aagcgatggc aggggcaatc aaaggcaaag
     1741 tgatgacaaa aaagattttc agcggacgcg cgcccaaatc cattgcggct tcttcgagcg
     1801 attggtcaag ctcaaccaga cgcgaacgga taacaacggt aatgtatgcc atgcacagcg
     1861 tcgtatgtcc taagaaaata gtgaaaaagc cacgatcgaa gtagagatgt tgtaaccatt
     1921 cgctgccctg caaaaatatc tgtacctgaa taatcagcag cagcatagac agaccggtaa
     1981 tcacgtcggg catcaccata ggtgcggaaa tcataccggc gaacaaggta ctgccgcgaa
     2041 aacgtttaat ccgcgccatc gcatagcctg ccagcgtgcc caaaacgacg gcggcaagcg
     2101 aagacacaac ggcaatccgc agcgacagcc aagcggcttc caagatggtg tcgttttcca
     2161 gcaatgcgcc gtaccacttg gtcgaaaagc cgccccaaac ggttaccagc ttggattcgt
     2221 taaacgaata gatgaccaaa acaaccagcg ggatatacag aaacgccagc gacagtgcca
     2281 acatcagttt caagaaccaa gataatttgg atttctgcat tatttggctc cttcttccaa
     2341 ttcgcggttt tcataatgct gaaacagggc aatcggcacg accagcagcg cgaccatcac
     2401 gacggcgacg gcggaagcca gcggccagtt gttttgatcg aagaacgcct gccacaagac
     2461 tttaccaatc atcaggtttt ccgaaccgcc gaccagctcg ggaatgacga actcgccgac
     2521 agcagggacg aaaaccagca tggagcctgc aataatgccg gttttcgaca aaggcagggt
     2581 aatcgtcaag aacgatttga ccggccccgc gcccaaatcg gaagccgctt caagcaggcg
     2641 gttgtcgagt ttcaccagtt gcgtgtatag cggcagaatc ataaacggca gataggcgta
     2701 aaccatcacc aaattgagcg aaaaggcatt gtagaacaaa tccaaaggct cgctgataat
     2761 acccatttta atcaacaggt tgtttacaat accgttatgc ccgagcagac ccatccacgc
     2821 atagacgcgc aacaggaacg atgtccaaaa gggcagcata atggcaagca gcaaaccatt
     2881 gcggacagaa ggattggcac gagaaatcgc ataggcggtc ggataaccga ccaacaggca
     2941 aatcactgtc gtcatcaatg cggttttgat tgaagaccag taggtcatca gatagatatt
     3001 gctgttttca ctgtcgccga acgggttaag ggtatgccaa aaattttgga aaatatcggc
     3061 atagttttga taactgatgg tgatattcag acgacctaaa tcttcgtcga tgctggtcag
     3121 cggcgtaaac ggcggaatgg cgatttcttg ttcggcaaag ctgattttca gcacgatggc
     3181 gaacggaatc agaaacagca ccaaaagcca aatatacggt acggcaatca ccacacgctg
     3241 ccccgggcgg cggaacagtt tgtttttcag tttattaagg ttcattgcat tccccttaaa
     3301 tcaacggaac agtggcgtag gctggttttc cggccagctg atatacacgg tttcgtccca
     3361 agttggcggt gtaatgttgc gtacatacca gtaaggtgca ggaacttggc ttttgacgac
     3421 gcgcccgttg gcgagtttga tatggtatat ggcaaagctg cccaaatagg cgatttcttt
     3481 taccgtgcct ttcgcccagt tgtagtcgcc caaatattcg ggtttttctt tatataaatc
     3541 aatatcctct ggtcgaatac taacccaaag gtcctgctcg ctcggaccac ccaaaccgtg
     3601 atcgatgcgg acgtggtttt ccaaaccttc gcattcgata acggcatagt cggcatgatc
     3661 ttcaatcacc acaccgtcaa agatgttggt ttcgccgata aactcggcag tgaagcggct
     3721 gttgggatag tcgtacacgt cgctgggtgt gccgacttgc tgcaactgac cgtcagacat
     3781 aatggcgatg cgggtcgcca tcgtcatcgc ctcttcttgg tcgtgcgtaa ccataataca
     3841 ggttacgccg acttgttcca gcgtattgac caactcaagc tgggtttgtt ggcgcagttt
     3901 tttgtccaat gcaccgaggg gctcatccag cagtagaatt ttcggacgtt ttgccagact
     3961 gcgtgccaaa gcaatgcgct gctgctgacc accagaaagc tggtgaggtt tgcgtttggc
     4021 aaatttggtc atctgaacca ggcggagcat ttcttcgacg cgcgcggcga tttcgccttt
     4081 aggcattttg tcctgtttca gaccaaaggc aatgttttgt tctacggtca tatgcggaaa
     4141 aagcgcgtaa ctttggaaca tcatattgat ggggcggtca tagggtgcaa gtttggtaat
     4201 atcctgacca tcaaggataa ttttcccctg attggggctt tccatacccg ccagcatacg
     4261 cagcagcgtg gattttccgc tgccggaact gcccaaaagg gcgaagattt cgtgttgata
     4321 aatgtccaag tcgatgttat cgacagcgta attgtcacca aactttttca ccaagccttg
     4381 gatttgcaga taaggtttgg ctgaagacgc agtggttgcg gtcataatgg caatactcca
     4441 ataaaaaaac gagtaccggc aaaacggatt ttcgaatggg tgataaaaag ctgtttgatt
     4501 gctggcggga gttaaacgtt tgatgccgtc tgaaactctt gtaaagcgca cgggcagcat
     4561 gaaatggaac aagattccaa agaactttat attatattag tttatgcggt tttcgggcaa
     4621 tatagtggat taaatttaaa ccagtacagc gttgccttgc cttgccgtac tatttgtact
     4681 gtctgcggct tcgtcgcctt gtcctgattt aaatttaatc cactataaat atggatttga
     4741 gcttgtcgga aagcaacaga aaagaaaaca ccgcccattt ttctgggcgg tgtcggaaag
     4801 cgtaattatt tacgcagacc caagcgggta atcaacgcgc gatacgtatc gggctgggta
     4861 cggcgcaagt aggccagcag gcggcggcgt tggctgacca ttttcaacag gccgcgacgg
     4921 ctgtggtggt cttttgggtt ggctttgaag tggggggtca ggtcgttgat gcggaaagtc
     4981 aacagagcga cttgtacttc ggaagagccg gtgtcgcctt ctttgcgttg gaaatcttta
     5041 acgatttgtg ctttttgttc tacggtcagt gccataatga aaactccaaa aatataagaa
     5101 tccccatagg gattcagaca agtttgccaa gcctgaagac aacggcaaac tccctatgcc
     5161 caagatagga caacgtggca ttatgacaca attcccgcat ttctgcacaa tattttaaga
     5221 ccgtggaagt aaccgttaaa aatgccgtct gaagcattgt ctgcttgaga cggcaatgtt
     5281 cagacggcat atgcgcttca aaccacgatt tcttctttct tcttcggttc tttaccgata
     5341 ttgtcgcggc tcaaaccgaa cattagcaga agcgggctgg caaccaatac ggaagaataa
     5401 atgccgaaca cgatgccaat ggtcaacgcc atagaaaagc cgtgcaaggc cgcaccgccg
     5461 aacaccagca tagaaacaac catcgcctcg gtcgaaccgt gggtaatgat ggtgcggctc
     5521 atcgttgcgg taatcgcgtt gtcgatgact tccggcacgg catgtccgcg catcgccggc
     5581 ttgcggaagt tttcacggat acggtcgaag acgacgacgg attcgttcac agaatagccc
     5641 aatacggcaa ggatacccgc caagacggtc agcgaaaatt cccattggaa gaaggcaaag
     5701 cagccgagaa taatcacgat gtcgtgcata ttggcgataa tggcagatac ggcaaaacgc
     5761 cattcaaaac gcatcgacag gtaaataatg atgccgataa cgacaaaacc taaagccatc
     5821 aatccattac ttaccaattc ctcaccgact tgcgggccga taaattcgac ttggcgcaag
     5881 gtaacgtcgg gactgtcttt tttcagcaaa tccataacct gattggacaa ctgtgcggaa
     5941 gtaacacctt ctttgttcgg caggcggatc atgatgtgtt tgttcgtacc caatgcctga
     6001 acctgtacat cacctatttt cagcgtatcg aggcgttcgc gcatcttatt gacatccgca
     6061 ccctgctgat attggacttc cattaccgta ccgccggtaa attcgacaga gaaattcaga
     6121 cctctggtaa ccaaaaagaa tacggcggca ataaaggtaa ccaacgaaat aaaggtcgtc
     6181 agtttgccgt agctcataaa cggaatatcg cgtttgattt taaagagttc catagcttac
     6241 tccttgcctc ctgccatttc ggctttcggc ttccacaccg aaccgatgga aatattctgc
     6301 aatttgcgtc tgcgtccgta ccacagattg accaacgcac ggaatacgac gacggacgaa
     6361 tacatcgaag tcagaatacc caaacagtgt acgaccgcaa aaccgcgtac cgggccggaa
     6421 ccgaatacca aaagcgcgat accggcaatc agcgaagtca ggttggaatc gacaatggtc
     6481 gcccatgcgt gttggaaacc gagattgatt gcctgctgcg gcggcacgcc gacacgcaat
     6541 tcttcgcgga tacgttcgtt aatcaagacg ttggagtcga ttgccatacc caaagtcaac
     6601 gccagcgcgg ccatacccgg taacgtcaac gttgcctgca tagcagacaa aataccgatt
     6661 aggaacagta tgttggcact caatgcaatg gtagaaaaga aacccatcag acgatagtaa
     6721 accaccatga atgcagcaac gatggcaaaa ccccataaag tcgaatggaa gcctttttcg
     6781 atgttctcct tacccaaaga cggaccgatg gtacgttctt cgacaatctg catcggcgcg
     6841 gcaagagaac cggcacgcaa cagcaaagac gtatcattgg cttcggctgt cgtcatgctt
     6901 ccggaaattt ccacgcgtcc gccggtaatg gcagtacgga taaccggcgc ggttacaacc
     6961 tcggattttc cttggtcgat caaaaccatc gccatgcgtt tgccgacatt tgcggcagtc
     7021 agttcgccga aaatgctgcc gcccgcgctg tccaagctca gactgacggc aggtgcgccc
     7081 atttggtcga aactcggttg cgcatcgttg atgttgtcgc ccgtcagctc gacctgtttg
     7141 ctgattagca gagtttcggg acgatctccg ccgcttgaaa gcagctcata accgctcggc
     7201 acgttgcctt ccaatgcctc gcgcaacttg gcaggatcgt cctccaccat acgcaattcc
     7261 aaagtcgcgg tacggccgat gatgtctttt gccttggcag tatcctgaac gcccggaagc
     7321 tgcacgacga tacggtctgc acctgactgc tggatgacgg gttcggccac gcccaactcg
     7381 ttcacacggt tgtgcagggt agtgatgttc tgtttgaccg catcggaacg cactttattg
     7441 accgcctctt ccgaaagcgt caagacgata ttgctgccgt ctgaattcag cgttgcttca
     7501 ggaaacagct tgcgcaactg cggcagtgcc ttttgcacat cacctgcatc ctgcaaaggg
     7561 acggtcaggc tgtttccagc ctgacgcacc gtgccgctgc ggattttttc gcggcgcagt
     7621 tcgcggcgga tgtcgcccga ataacgttca aacgttttct gcatcgccgc tttcatatcg
     7681 acctgcatgg tgaaatgcac gccgccgcgc aagtccaaac ccaaaaacat cggattggct
     7741 ttgattttcg ccatccattc ggggctgtcc gccaacaggt tgagcgcggt aatataccct
     7801 tcgcccaaag tgttttcgat gacgtcgcgc gctttaagct gcgtttctgt gtctttgaaa
     7861 cgcactttca gtgaattgtc cacaacaaac atcccgtcgg tctgaatacc tgcgtttttc
     7921 agcgcggcat ccactttgaa ttgagtctgt tcgttgatga tgatggcttg tcggttggtc
     7981 gatacctgca cggcgggtgt ttcgccgaat aggttgggca gcgaatacac tgcggcaacc
     8041 gcaatcgtga acacaatcag cagatatttc cataaaggat aacggttcat cattgttcct
     8101 taatggttgg aaccccaccc tttcggtggt gtcggaatcg ggctatttca gaagaggcaa
     8161 aaacccttcc cagccaggca agaccggaag gcggcatctt gaatatgccg ccctgcgtgt
     8221 cggaacatgg tcaagccttc ggttggaatt caaaacaaag tgccgcattc gggctttcca
     8281 gatgcggctt gtcggcacaa atcaatcgac ttttgcggca atcgcattgc gttctacttc
     8341 gacctcgatt tttgtaccct gtccgatatc cacggtaaaa aactgttcgc cgactctggt
     8401 taccttaccc ttgaaacctg ccgccaagac cactttgtcg ccgactttca aggcggcaag
     8461 cattgcctga tgcgctttga atttcttttg ctgcggacgc atgatcagga agtagaacac
     8521 caccataatc aacactaaag gagcaaattg tgcaacagct tgattcataa tttatccgtt
     8581 ctttctaata tggttgaaaa tcgagagggg tatataataa cataagaccg taaacaatat
     8641 atcgggtttg ccgtccgtac cgaccgtata ccgcagcctg cccgtccaca aacccatgtc
     8701 cttgacccgc ctaatcttga aattctatgc actgttgcgc ctttttttgg gcaaaaacgc
     8761 ccgcaccgca tggatttcgc atcccgcctg tgccgggcac gaacccggcg caaaccatcc
     8821 cgattcgccc gaccgcatcc tctgcatcga gcaggcattg cgccgcgccg gtatttggca
     8881 gcacctccaa accatagagg cggaagaaat cagcgatacg cgcctcgcac ttgtccactc
     8941 gagcaaatat ctgaaccgtt tggaatcttg cctgccccaa aaaggcaaga tttcccgcct
     9001 ggataacgac actgcaatca gcacaggctc gctgtctgcc gcacgctttg ccgccggttc
     9061 ggcagttcag gcagtcgaca tggtcatgaa ccgtaaagca tggcatgcct tttgcgccgc
     9121 ccgcccgccc ggacaccatg cgggcagcgg caaagccggc ggattctgcc tgctgaacaa
     9181 cgttgccgcc ggcgtcatgc atgccgttgc cgaataccgg ctgaaccgca tcgccgtcat
     9241 cgatttcgat gtacactacg gcgacggtac ggcagaaata ttcaaagacg atccgcgcat
     9301 cctgtttttc aacctgtttg aaaccgacct tttccccttc cccgaaaaca acgatatgcc
     9361 cgacggcggc aatatggtgc acctgccctt gccgccggga acgggcagcc gcgcattccg
     9421 cgaagccgtc cgcagacagt ggctgccccg gcttgccgca ttcaaacccg aactggtgct
     9481 gctgtcggca ggattcgacg cacaccgtct ggacgaatcg ggcaggctta acctgcacga
     9541 ggcggatttt gcctggctga cacacaaaat cattcagacg gcatcgggct gccctggcaa
     9601 aatcgtatcc gtactcgaag gcggctacac ccttgaacct ctggcacaat ctgctgccga
     9661 gcatatccgc gtgctggcag ggctgggcaa atccgatgcc gcaaccgcct atcagaaaac
     9721 actggaccca acaaaaaaac ggtttgccaa accgaaaacc ggacaggtgc gccaacctac
     9781 ccaatccgac cgttacg
//
LOCUS       DAWFAN010000067         9771 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWFAN010000067 DAWFAN010000000
VERSION     DAWFAN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.067"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(314..547)
                     /locus_tag="WGO65_001155"
     CDS             complement(314..547)
                     /locus_tag="WGO65_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ0327450.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(713..913)
                     /locus_tag="WGO65_001156"
     CDS             complement(713..913)
                     /locus_tag="WGO65_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ0327451.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1085..1960)
                     /gene="xerD"
                     /locus_tag="WGO65_001157"
     CDS             complement(1085..1960)
                     /gene="xerD"
                     /locus_tag="WGO65_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ0327452.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGSVDLDRG
                     CITALGKGDKQRMIPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2012..2452)
                     /locus_tag="WGO65_001158"
     CDS             complement(2012..2452)
                     /locus_tag="WGO65_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ0327453.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2773..3711
                     /locus_tag="WGO65_001159"
     CDS             2773..3711
                     /locus_tag="WGO65_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ0327454.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKKLLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3840..4133
                     /gene="hfq"
                     /locus_tag="WGO65_001160"
     CDS             3840..4133
                     /gene="hfq"
                     /locus_tag="WGO65_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ0327455.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4218..4784
                     /locus_tag="WGO65_001161"
     CDS             4218..4784
                     /locus_tag="WGO65_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0327456.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNNPPYLLAFEKLRQK"
     gene            4838..5212
                     /locus_tag="WGO65_001162"
     CDS             4838..5212
                     /locus_tag="WGO65_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ0327457.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5222..5716
                     /gene="folK"
                     /locus_tag="WGO65_001163"
     CDS             5222..5716
                     /gene="folK"
                     /locus_tag="WGO65_001163"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ0327458.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPCAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(5843..6322)
                     /locus_tag="WGO65_001164"
     CDS             complement(5843..6322)
                     /locus_tag="WGO65_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ0327459.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(6542..7279)
                     /gene="ubiE"
                     /locus_tag="WGO65_001165"
     CDS             complement(6542..7279)
                     /gene="ubiE"
                     /locus_tag="WGO65_001165"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ0327460.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7322..7690)
                     /locus_tag="WGO65_001166"
     CDS             complement(7322..7690)
                     /locus_tag="WGO65_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ0327461.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7810..9429
                     /gene="mnmC"
                     /locus_tag="WGO65_001167"
     CDS             7810..9429
                     /gene="mnmC"
                     /locus_tag="WGO65_001167"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ0327462.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRALLRHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGANSPY
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAQLNPTLAESLFAANPPPQKHQGHAAIRCDSPDHLPVVGAL
                     GNIAAMQQTYAKLALDKNYRIDSPCPYLPNAYANTAHGTRGLATAPICAAAVAAEILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
BASE COUNT         2606 a         2979 c         1966 g         2220 t
ORIGIN      
        1 tctaccgatt acacaaccac atatcatctt tacacaatca tgcttccatc aacagcaaaa
       61 catgatatga ttgctgctaa taacatctca caataaattt tctaattttt attgaaaaaa
      121 tcaataaata agaattctcc caaccgacaa ataaaataaa gaaagggtca atatgcaaca
      181 tcgaagaaga ctggcaattt accaggcatc caaacgtgct tcctttaccg gcaggtcatc
      241 cgccccgcaa aaacgtaaag aacgttgatt tgaaaaaaat gccgcctgaa gtcctgcttc
      301 agacggcatt tttttaccgt tcgagaaact gtttcaacct gtcctcatcc aaaaaccagt
      361 ggcagatttc ctcatctccc gccaacaaaa cgggaaccag ctcattgtat ttttcttcca
      421 aaacaggatt ttcatccaca tcgaccactt ccagcccgaa cccgtattca ttttgaaaag
      481 gtttgagtgc atcgcgcatt ttgtggcaca agctgcaata ttcacgaaac atcaaggtca
      541 atttcatccg cgtttcctta tctgtcaatt tgcacacgcc aaagccttag acgcagcaga
      601 atcatggtct atttgggaaa aaacaatgtt ttcaaggaag atgatactca agtcctgcca
      661 aaaacagcaa aaatgccgtc tgaacagttc agacggcatt tccaaaaccg ttttacgctt
      721 gaacattgat acccgccgta accgtcggtt gcgcattgtg cgccgtcaga aacgaagcgg
      781 caagctcccc acagcagccg cagcagctcg ctacgcccaa ttgcgactgc aaatcgccca
      841 ttgtggtcgc gccggcggcg atggtttcct tgatttgatg gtcggtaacg gcattgcaga
      901 tgcagacaaa cattttgtgc tccgtgtgtt ctcaaactat cgtaccgata gcggctttta
      961 ttatcgtatg caaatataaa taaaaacggt tcgcattgca aggtcggcat acacggtttg
     1021 tcttggtaat tttttatcaa gttttcattt ccgattgatt tcttattaaa ccgaagcaga
     1081 agcctcagtt tcgggaatgg tgttccttca ctacaccctg caaccatacg ttggcaacat
     1141 gggtataaat ctgcgtcgta ttcaaatcgg catgtcccaa catatcctga accacgcgca
     1201 aatccaagcc gtgccgcacc agatgcgtgg caaaggcgtg gcgcaggctg tgcgggctga
     1261 tgtgcccgat gcctgcctga cttgcatatt ctttgacaat catccatgcc aactgacggg
     1321 aaatgcccgt ctttttctga ctgacaaaca atgcgtcgca attcctgcct ttcagcagaa
     1381 gtgggcgtgc ctccgtataa tagcgttcca cccaatacgc cgactcctgc cccatcggga
     1441 tcatcctctg cttgtcgccc ttgcccagcg cggtaataca gcccctgtcc aaatccacac
     1501 tgccgaaatt cagcccgacc gcctcgctga cgcgcaagcc ggtcgcgtac atcaattcga
     1561 gcagtgcctt gtcccgcaaa ccgtgtggcg tatcagtatc cggagtggca agcagccggg
     1621 aaatctgctg ttctgtaatc agtgtcggaa tattcttgtc gattttgggc ggtttcagca
     1681 aacgggtggg attgtccgtc cttatgcctt cacgctccat ccatatatac aggcgtttgc
     1741 acgccgataa tgcgcgcgcc tgcgaactcc gttgctctcc gtcaacataa accgccgccg
     1801 ccaaatccgc ttcgtccgca tccttcagca ttctgcccga ttgggacagg cggcgggcga
     1861 ttttttccaa atcgcgccgg taaccgttta aagtattctg actgagccgc ctgtccaacc
     1921 acagcgtttc aagcagcctg tcgatcaaac cttcttccat accgttccaa acaaatgccg
     1981 tctgaatctt cttcagacgg catggttcac attatcggga aagcgtttcc aatacttcct
     2041 gcgcgtgacc cgccactttg actttccgcc attcatgggc gatttctcca tccttattca
     2101 agacgaacgt actgcgctcg atacctaacg actctttccc gtacagtttc ttcaatttga
     2161 tgacatcaaa caggcggcac actgtttcat ccttgtcgct caacagctcg aaccggaaac
     2221 cctgcttggc gcaaaaattc tgatgcgcct ttacgccgtc gcgggaaata ccgaccacgg
     2281 tataacccaa tgcctcaaac tgttccaaac gcgcattgaa atccaagcct tccgtcgtac
     2341 agcccggcgt actgtctttc ggataaaaat acacgaccaa aggcagatgt tctgccgaat
     2401 gaaaatccgc accgctgctc gaaggcaggg taaattcata tttcacatcc atagtcctac
     2461 tcccgatatt cccattattc aaaacgacac gcagacgcac cgccgcaatt gccaaaccaa
     2521 ccccgattct accgccccaa aggacaggga ttcaaccgcc ggaaacatcc aaaccgacac
     2581 acgacggcat gaaaaatatc catgtcaaac cacaaaatat gttccgattt aaaaacagaa
     2641 tgttataaaa cccaatcccc aaaacacaac aagaccgccc gcctacgggc agtctcctgt
     2701 cagacgacat actttacaga tggctgtttt ttcaacaaaa taacgccaat actcaaaaat
     2761 atggaatcaa aaatgtccat ccatactctg aaacgcctgc cctcatcgct gctgctcggt
     2821 ctctgccttt ccctgccgtc agcccacctt tttgccgaca acgacatttt agggcaattt
     2881 ttagaacaga acatgcttac ctcctccgat ccgatagaaa tattcgccga aagcacgata
     2941 caccccacca acacccaagc cattacaggc ggtctgattc tctcctcaca gtctgccctg
     3001 gtcgtcaaca acaaaaccgg acagatactg tatcagaaaa acgccgacag gattatgccc
     3061 atcgcctcca tttccaaact gatgagcgcg atggtcgttt tggatgcaaa cttggacatg
     3121 aacgaaaccg ttaccattac gcccgacgaa atcgaccgca tcaaagggac cggcagccgt
     3181 cttgccatag gtacggcact tacacgcaaa aaactgctgc acctgagcct gatgagcagc
     3241 gaaaaccgcg ccacccatgc attgggcaga acctaccccg gcggcatggg cgcatttgtc
     3301 gccgccatga accgcaaagc ccaaagcctc ggtatgtacg gcagccgctt ttacgaaccg
     3361 accggactca acttccaaaa cgtttctacc gccaaagacc tgagccttat ggtcaacgcc
     3421 gccgcccaat atccgcaaat ccgcaccaac tcgacttcca actacgcctc ggtacagacc
     3481 aaaaacgggc agcagaacta caaaaactcc aatgccctgg tcagagaagg catgtggaac
     3541 atcgaattgc agaaaaccgg ctacatacgc gaagcaggca ggtctatggt tgtcaaagcc
     3601 aacattcaaa accaacccgt taccatcgta ttgctgaact cgcccacatc cgccacacgc
     3661 gtcaacgacg cccgcaaaat cgaatcgtgg atgctgcagc aacgctcctg acatacaaat
     3721 gcccggcgga aaaccgccca aacacaaaaa aaattggtaa tttttcctat tcaaggctac
     3781 aattcgacac gcacagggcg tctgcccgat ttcacaatca taataacgga gttaaaaata
     3841 tgacagctaa aggacaaatg ttgcaagatc ccttcctgaa cgccctgcgt aaagagcacg
     3901 ttccggtttc gatttactta gttaacggta tcaaattaca aggtcaggtt gagtctttcg
     3961 atcaatacgt tgttctcctg agaaacactt ccgtcaccca aatggtttac aaacacgcca
     4021 tttccaccat cgtaccggca cgctccgtca acctacaaca tgaaaacaga ccccaagccg
     4081 caccgacttc gaccctcgtc caagtggaaa ccgtccagca gcctgccgaa taatccgcac
     4141 gaagcatgac gtgtcatatc tttcaatacc ttaccggaca acggtaaggt atttttattt
     4201 tcagacagca tttaaaaatg ttattgcaaa acatccttcc attcgcccat tgccttttgc
     4261 ggaaggcact tcccgaaggt ggcaatgctt tggacggcac cgccggcaac ggacacgaca
     4321 cccttttcct cgcacaaacc gcaggcatcc gggggaaagt gtgggcattc gacatccagc
     4381 cgcaagccct gagccgcact cgatgccgtc tgaaagaagc gagatacgag aatgtgcaac
     4441 tcatcttaga cggacacgaa aacctgaaac aatatgtttc agaaccacta aatgccgcca
     4501 ttttcaactt tggctggctg cccggcggag ataaaagcct taccacccat acggaaacca
     4561 gcattcccgc cctttgcgca accctctccc tgctgaaaga aggcggtatg cttattgccg
     4621 tcctctatcc gggacacgaa aacggcaaac aggaggcaga agcaatcgaa caatgggcaa
     4681 aaaacctgcc tcaagaacag tttgccgttt tgcgttacgg ctttaccaac cggaaaaaca
     4741 acccgcccta tctgttggca tttgaaaagt tacgtcaaaa ataaatgttt gcggtaaaat
     4801 aagccgattc taacccttat tttgatacga gccgaccatg cagtatctga ttgtcaaata
     4861 cagccatcaa atcttcgtta ccatcaccat tttggtattc aacatccgtg ttttcctact
     4921 ttggaaaaat ccagaaaagc ccttggtcgg cttttggaag gcactgcccc accttaacga
     4981 caccatgctg ctgtttacgg gattgtggct gatgaaaatt acccatttct ccccgttcaa
     5041 cgcgccttgg ctcggtacaa aaatcctgct tctgctcgcc tatatcgcat tgggtatgat
     5101 gatgatgcgc gcccgtccgc gttcgaccaa gttctacacc gtttacctgc tcgccatgtg
     5161 ttgcgtcgcc tgcatcgttt accttgccaa aaccaaagtc ctgcctttct gaaacaccgt
     5221 tatgaacaac agacattttg ccgtcatcgc cctgggcagt aatcttgaaa accctgccca
     5281 acaggtacgc gccgcattgg acacgctgtc gtcccatcct gacatccggc ttgaacaggc
     5341 ttcctcactg tatatgaccg cccccgtcgg ttacgacaat cagcccgatt tcgtcaatgc
     5401 cgtctgcacc gtttccacca ctctggacgg cattgccctg cttgccgaac tcaaccgtat
     5461 cgaagccgat ttcggacgcg aacgcagctt ccgcaacgcg ccgcgcacat tggatttgga
     5521 cattatcgac tttgacggca tctccagcga cgacccccga ctcaccctgc cgcatccgtg
     5581 cgcgcacgaa cgcagtttcg tcatacgccc tttggcagaa atcctccctg attttgtttt
     5641 aggcaaacac ggaaaggttg ccgaattgtc aaaacggctg ggcaatcaag gcatccgtct
     5701 tttaccggat aagtaattcc gcacgcggat gccgtctgaa tgccggtttc agacggcatt
     5761 tttcctttgc cgccaacacg cgtgcaaaaa aatcgcccct tggaaaaggg gcgcaaaagg
     5821 aacacaaacc actaccaaaa ctttaaatct gcaacattgc ctgccgcata ctgtatccga
     5881 caggatataa agccctcact aaatcgtttc gagaaatcca aacttcttca tcgccgacag
     5941 aaaatctgcc tttctccggc accagctcca acaaaaacgg ttgaaccgcc gtatgcagcc
     6001 tgtccttaca ccgccccagc tttctggaca aggcggacag ggcacgctta tagacactat
     6061 ccttgcggtc aagttcccgc gcactgccga cccagctcaa acgaaggttg cggctttcat
     6121 cctcaatcag caaaatgcct gttttgactt cccccacctc tggctcgcat gaaagcgaaa
     6181 tataatattt gccgtcgatg ttgacatacg ccgcctcatg cacctgacag ttgcagccgg
     6241 aagtggcaag cagcgaataa acagaatccc gatgcagttt caaaatatca agggaaatct
     6301 gtttgaaaat actttccggc atttttccct cctttcgtct gctgcgttgt ttactggatt
     6361 gactgcatta tacagacatc tcccaatctt gcaaatgata attgttccta ttttttaaaa
     6421 aatttataaa ttattgatag atatgtgaat taattttaag atttaaaatc tctttctcaa
     6481 aaaaacacaa aaatgccgtc tgaaacgtgt tcagacggca ttggctgcac agccagtccg
     6541 tttaaaattt cacgccctta tgcagcgcaa cgatccccgc actcatatta tgatagtcca
     6601 cactgtcaaa tccggcatcc agcatcattt gctttaaagt ttcctgatcg gggtgcatac
     6661 ggatggattc ggcgagatac tggtagctgt ccgcatcttt ggcaatcagt ttgcccatga
     6721 ccggcagcag cttgaacgaa tagaaatcat acgcgccttc gagcggcttg tagattttgg
     6781 aaaactccaa caccagcaac gtgccgccag gtttcaaaac acgatacatc tctttcagcg
     6841 cggcatcttt atgcgtcatg ttccgcaagc cgaacgccac ggaaactaag ttgaaataat
     6901 tgtcagggaa aggcagtttt tccgcatcgg caagcgacac gggcaaaatc atgccttcgt
     6961 tcaacagacg gtcgcgcccg acggtcagca tagaggaatt aatatcggtc agccaaacct
     7021 cgccttcctt gccgacccgt ttcgcccaac cgcgcgacaa atcgcccgta ccgcccgcaa
     7081 tgtccaacac tttatcgcct tttttcaggt gcgccgtgtt gatggtgaaa tgcttccaca
     7141 ccctgtgcag ccctgccgac atgacatcat tcataatgtc gtagtttttg gcgacggagt
     7201 ggaacacttc cgccactttg ccggcttttt catcttcgtt gaccgtgcta aacccgaaat
     7261 gcgttttttg tccgcccatc ttctgaccac ctccctgatg ccgtcctccg gcatcccaaa
     7321 cttaacaatc tgattgcttt tcaaaacgcg acgcaccagc cgccgccaat ttgtccaaat
     7381 attcctgcca catcgcatcg tatccgtatg ccagtctgtg cagatacgcc caatcgtaaa
     7441 gaccgctgtc gtgcccgtcc gaaaaactga ttttcagcgc gtactgtccg acaggctgca
     7501 aatccgtaat ttgcacatct gccttaccgg tctgcaaaac atcctgtccc acaccgtgtc
     7561 cgcgcacttc cgcactcggc gaatagacgc gtaaaaattc ggcaggcaga tttttaggtt
     7621 cgtccccata agtcaaaacc aacacggtac ggtttttcaa caaacggatt tcaacaggaa
     7681 tattattcaa aaaacacctc ggttcggcat acaatatccg gcaggcgcag gcgtgccttc
     7741 aaacggcgcg tattttaaca gctttatccc gataccgata ttcaaaccca catttcagac
     7801 ggcattgata tggacaatct cgtatgggac ggcattcccg acatccgcac actcgaccaa
     7861 accatccgca aacacgcaca cccgctcaac ctgattgtct gcctccccga taatcagatt
     7921 cccgattttc aaaccgcaca agatgcttcg gacgcggaat gccgtctgaa gcaccgtttg
     7981 gatcaggcaa cgcagtgcct ccagttcgac agcatcaacc tcatcgaaca catcctgccc
     8041 gatgtccgct tctggctggt tcccccttca cgcacccgcc gcctgcacga acacttccac
     8101 cacatttcct ggcagaccga agccatcccg caaaccgaaa gtaagcccga caaaccctgg
     8161 tttgcacttc cacaaacatc cgaacggcaa aaaccggaac acatcctcgt tatcggcgcg
     8221 ggcatatccg gcgcggcaac cgcccacgcc ttagcatcat acggcatttc cgttaccgta
     8281 ttggaagccc gaaaagccgc ccaagccgcc agcggcaacc gccaagggct gctctacgcc
     8341 aaaatctcgc cgcacgacac cgaacaaacc gaactgctgc ttgccggcta cggctacacc
     8401 aaacgcctgc tcggacacat cctgcccgaa tccgacactt ggggcggcaa cggcatcatc
     8461 cacctcaatt acagccgcac cgaacaacaa cgcaatcacg aattgggttt gcaaaaacac
     8521 cataaccacc tctaccgcag catcacgcag gcagaagccg aaaaaatcgc cggtatcccg
     8581 ctgtccgtcc catacgacca cccttcatgc ggactctact ggcaacacgg cgtatggctc
     8641 aatccgcccg cattcgtccg cgccctcctc cgccatccgc tgatcggact gcacgaagac
     8701 acaccattaa ccgacatttc ccacgacggg gaaaagtgga ttgcaagcac gccaaacggc
     8761 acatttaccg ccacacacat catctactgc accggtgcga acagccccta cctacccgaa
     8821 accaacctcg ccgccctgcc tctcaggcaa atacgcggac aaaccggcct cacaccgtcc
     8881 accccgtttt ccgaacaact gcgttgcgcc gtttcaggcg aaagctacat cagcccgtcg
     8941 tggcacggac tgcactgcta cggcgcgagt tttattccca acagcagcca tactggatgg
     9001 aacgaagccg aagaagcctc aaaccgccaa gcattggcac aacttaaccc cacccttgcc
     9061 gaatcattgt ttgccgctaa cccacccccc caaaaacacc aagggcacgc cgccatacgc
     9121 tgcgacagcc ccgaccatct gcccgtcgtc ggcgcactcg gcaacattgc cgctatgcag
     9181 cagacctacg ccaaactcgc gctggacaaa aactaccgca tagattcccc ctgcccgtac
     9241 ctgcccaatg cctacgccaa taccgcccac ggcacacgcg ggcttgccac cgcccccatc
     9301 tgcgccgccg ccgttgccgc cgaaatccta ggcttgcccc atcccttttc acaacgcctg
     9361 cgccacgccc tacaccccaa ccgcaccatc atccgcgcca tcgtcagaag gaaggatcta
     9421 accccttaag cctttgccgc atgccgtccc gaccacagaa aatcatattc aaataaacat
     9481 accaatcctg acggtaaaat atcgaaaaca cccataacgt tttcttccca aaataaggag
     9541 taaggaatgc ctaacaaatt gcctttgctg tccaagatga atattccgta acattcgaag
     9601 acttccttaa ccctgccagc cgtttcggtt tctgtacccc ctgttagata aggaaggaat
     9661 gaaaaagccc tgttgattgt atgaagtaaa gggcgttaat ttttgttaat cttcccttct
     9721 caggaactca atatataagg atgttaattt ttatttccct tctcgaggac t
//
LOCUS       DAWFAN010000068         9641 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWFAN010000068 DAWFAN010000000
VERSION     DAWFAN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.068"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..546)
                     /locus_tag="WGO65_001168"
     CDS             complement(<1..546)
                     /locus_tag="WGO65_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0327463.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIP"
     gene            complement(578..961)
                     /locus_tag="WGO65_001169"
     CDS             complement(578..961)
                     /locus_tag="WGO65_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0327464.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            1330..2034
                     /locus_tag="WGO65_001170"
     CDS             1330..2034
                     /locus_tag="WGO65_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ0327465.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASYGVEGTDSVA
                     PGVEEISISVNGTVQF"
     gene            complement(2099..2665)
                     /locus_tag="WGO65_001171"
     CDS             complement(2099..2665)
                     /locus_tag="WGO65_001171"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ0327466.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2731..3146)
                     /locus_tag="WGO65_001172"
                     /pseudo
     CDS             complement(2731..3146)
                     /locus_tag="WGO65_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3208..4107)
                     /locus_tag="WGO65_001173"
     CDS             complement(3208..4107)
                     /locus_tag="WGO65_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ0327467.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(4138..5595)
                     /gene="der"
                     /locus_tag="WGO65_001174"
     CDS             complement(4138..5595)
                     /gene="der"
                     /locus_tag="WGO65_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ0327468.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5745..6374)
                     /locus_tag="WGO65_001175"
     CDS             complement(5745..6374)
                     /locus_tag="WGO65_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ0327469.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQNEINAELAKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6375..7670)
                     /gene="hisS"
                     /locus_tag="WGO65_001176"
     CDS             complement(6375..7670)
                     /gene="hisS"
                     /locus_tag="WGO65_001176"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ0327470.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRTQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            complement(7797..8339)
                     /locus_tag="WGO65_001177"
     CDS             complement(7797..8339)
                     /locus_tag="WGO65_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0327471.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVGQIRQARAE"
     gene            complement(8511..8702)
                     /locus_tag="WGO65_001178"
     CDS             complement(8511..8702)
                     /locus_tag="WGO65_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327472.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(8735..9271)
                     /locus_tag="WGO65_001179"
     CDS             complement(8735..9271)
                     /locus_tag="WGO65_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327473.1"
                     /translation="MKKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(9439..9639)
                     /locus_tag="WGO65_001180"
     CDS             complement(9439..9639)
                     /locus_tag="WGO65_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327474.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         2254 a         2308 c         2456 g         2623 t
ORIGIN      
        1 cggtattttc ttaccgtatt ctgcgattgc ctgtcggaaa atgccgatca acctgcctat
       61 aacggcattt tcgccaaatt cgttcagaca gttttcccta agtcgggcag gttcaaaatc
      121 agagtggtgt tcatacattt tgatgagtgc gtcggcaagg gcatcgtcgt cgtcaacagg
      181 aaccaaatat ccgttgccgt ctgaaacaat agattccgca ccgccgcagc gtgtcgcaat
      241 gacgggcaat ccttgggaca gtgcttcgat atagaccacg ccgaaggttt cggtgcggct
      301 ggcaaggacg aatgcgtcgc tgttcctcat caaatccaag actgcttcgg gctgcaatgc
      361 gcccaaaaat gtaacggcat gggtaatgcc caagtctgcc gcctgctgtt tcagccgctg
      421 ttcttcctgt ccgctgccgc cgatgttcag gcgcagttgc gggcattgtg ccaacgcccg
      481 ggcaaaggca gtgagtagga catcgtgtcc tttgagacgg cgaaggtgcg agacggtgca
      541 gaacacgaaa tgcggtttgt tgtttttttc tctttgttca aacggtcggt tgaatattcc
      601 gcccagtatg ttgggggagg tactgccatt cgcagccgta tttgtgttgc aggacgtgtg
      661 cgaaatggcg gctgacagcg agcagtgcgg aggcgtgcgc cgctgctttt tccatcggct
      721 gccattggtg gggacggatc agaccgcgcg tgatggtgct gctgtgttcc gtgacgacat
      781 aggggatgcc gtatttttgg gaaatttttt gggcgagtat gccggcatag ttcatgcggt
      841 gggcgtgaat caggtcgggc agcccgtttt cgcggatgta gtgtttgaaa gctttcaaac
      901 ccgcacacac ccagcggatg cagtcgatgt cgatgaacgg aaagcggggg aagaaatata
      961 tgccgtgcca tgcatagatg tccaaaccgc tttgccgata ttttgcaaaa ccgtaaggcc
     1021 ggtcaggatg cttgctgttt ctttccgcaa gtagcggaac atcggtgcaa gcacggcggt
     1081 tttgatgcct ttcctctgca atgccagtgc ctgattttga aaaaaatccc gtccacatcc
     1141 tgttcggatt gcggatacca tgaggggatg acgaggacgt gcaagggttc gggcatagtg
     1201 ggattccgta tcggaaaggc ggttattata agacagacgc agaccgaata tttaaattgt
     1261 tgccttacgc taatgcaatt tagcgcgccg atatgctaga ttggcggttt tatgagtgag
     1321 gatacggata tgttgcgttc tattttggcg gcttccctat tggcggtatc ttttccggca
     1381 gcggctgaag cattgaatta caatattgtc gaattttccg aatcggcggg tgtcgaggtg
     1441 gctcaggata caatgtccgc acgtttccaa gtggcggcgg aaggacggga caaaaatgcc
     1501 gtcaatgccg agtttgttaa aaaattcaac agttttaaca gaaaatcgaa aaacggcagc
     1561 tttaaaaccg aattggtatc gcgcagtgcg atgccgcgct accagtacac caacggcaga
     1621 cgcattcaaa caggctggga ggagcgtgcg gaatttaagg tcgagggtag ggattttgat
     1681 gcgttgaacc gttttattgc cgatgttcag gcagatgccg cgttggaata tacggatttc
     1741 catgtgtcgc gcgaacgccg caacgaggtc atcgatcagg tcagcaagga tgccgttttg
     1801 cgtttcaagg cgcgtgccga aaagttggcg ggcgttttgg gtgcgtccgg ttataaaatc
     1861 gtcaaattga atttgggaca catcggcagc catatcgcgg gtgggggagc tgctcaggca
     1921 aaaatgcttc gtgccatgcc gatggcggca agttacggcg tggagggtac ggattccgtt
     1981 gcacctggtg tggaggaaat cagcatcagc gtcaatggga cggttcagtt ctgatttgag
     2041 gtgaacggta aatgccgtct gaaacccgac gataagggtt cagacggcat tttgtgtttc
     2101 aggctttggg cagggttacg ccggtttgtc ccatgtattt gccgttgcgg tctttgtatg
     2161 aagtttcgca cacttcgtcg ctctcgaaaa acaggacttg cgccacgcct tcgccggcgt
     2221 agattttggc gggcaggggg gtggtgttgg aaaattcgag ggtaacgtag ccttcccatt
     2281 ccggttcgaa cggggtaacg ttgacgatga tgccgcagcg tgcgtaggtc gatttgccca
     2341 agcagacggt caggacgttg cgcgggatgc ggaaatattc gaccgtgcgc gccagtgcga
     2401 aggaattggg cgggatgatg cagcagtcgt cttcaacggt aacgaagttt tttgggtcga
     2461 agtttttggg atcgacgatg gtgctgttga tgttggtgaa gattttaaat tcgtttgcgc
     2521 agcggatgtc gtagccgtag ctggacgtac cgtaggagat gatgcgtttg ccgtcggctt
     2581 ctttgatttg gttcggctcg aaggggtcga tcatgccgaa ttcttcgctc atgcggcgta
     2641 tccatttgtc ggacttgatg ctcataatgt tttccttgtt tcttgcagtg ttcggacaaa
     2701 gcattggggg atgccgtctg aaaacggggc ttatttgttt ttaggcagtt tcacttcttt
     2761 aatcatgccg ttttcgcatt tcataatgaa gagggtttcg ccgttttgcg agacggtaaa
     2821 tctgccgtgt gccaagcctt ttttgaacgt gcccgagagt accatgttgc ggaatttggt
     2881 actgtcggaa ttgaacggtt cgataaatat ttcgcggttg gcggcaacgg tataaacgcc
     2941 ttgtccgtcg aatttgccat ttttaaacga accggtatag ttgcgcccgt cttggcagcg
     3001 catgtgcctt tgcctgaagg aagcccgtct tttccgactc cgccttcgta ggtgcagccg
     3061 ggttcttgat aggaagtcag aacggcggcc gaagtgggga gggcgaacat catggcgggc
     3121 agtaggaatg cgagatgttt aagcataagg gttattccat tggattttgg ttgacggtat
     3181 tttgtcgtga aaaagccgtc tgaaaaatca atcttgccag ccgcccaaat aggaaaccag
     3241 ttcttccaac atggtgctga cggattccgc catcagaatt tgcgaggcga aggcaaggcc
     3301 ggcggcatcg tcgccgttgc tttcggcttc ttcctgcaat acgtcgaggt attggatgcg
     3361 cttgagtgtg aagtcttgag tgaggataaa ggcaatttgt tcgcgccaca ccaagcccag
     3421 ttgggtaacg gttttaccgt ttttgacgtg ttgaaccact tcgtcggcgg ttaaatcttg
     3481 tttggatact ttgacgacgg gaacaatatc gcccgtacct ttgagttcgc aatcgctgtc
     3541 taattcaaaa ccgccttcgc aatgcccttg caacagccag ccggtcatca aggaagaggg
     3601 cgattgcttg gtattcggca gcgaggcttc caaaccgccc aaagcttcgc gcagcttggt
     3661 caggatgttt tctgctttgg cggaagccgc gttattgacg agcaggtagc cgtggcgggt
     3721 gttaaacacc gcttctgtac ggctgctgcg ggtaaacgct cggggcagca ggtcgtctgt
     3781 aatttgctct ttaagctctt gtttttcttt gcgaccgaca ttgcgggctt cattgttttg
     3841 gatttccgct accttctctt ccaaaatatc gcggatgacg ccggcaggca ggactttttc
     3901 ttcttttttc agggcgacgc gcaaggtaaa gtcggcaggg aaaacgagtt caggggagaa
     3961 tgaaaccggt gcggtaaagc cttcgctgaa ccagtctaag ccttggcaat gggtaaattc
     4021 agcttcagca agtttgtcgg caagtacgtc tgcctcaggc agcttttctt tgttgagcgg
     4081 ataaaaacta atctgcttga accacataat gtttcctatt gtttgaaatg tcgggaatta
     4141 tttgctgaat tgttttttca cactgacttt ggttttcttc ttgaagcggt ttttctcttc
     4201 ctttcggcct tcgcgtttct caatacggtt gctcaggctg acgcggcgca gcggtttttt
     4261 cttcggtttg tcttccgcat tttcatacgg gttttccgaa acattgtatt gaattctgag
     4321 cggcgtgcct tgcagattga aggctttgcg gaacgtttgg gtcagatagc gcgtatagct
     4381 gtcggaaatc gcgtgcagcg aattgccgtg taccacaatt acgggagggt tcatgccgcc
     4441 ttggtgggca taacgcattt tcggacgcac caagccggca cgcggcggtt gctgacgctc
     4501 gatcgcgctt tgtaatacgc gcgtgatttt cggcgtcggc atcttaatca tcgccgcgtt
     4561 gtaggcagcc tgaatgctgt caaacaaacc gtctataccg cgctctttca atgcggaaat
     4621 aaagtggaac ttggcaaaat cgaggaaata cagtttgcgg ttgatatcgc gtttcacttg
     4681 ctcgcgacgc tcttcgctga tgccgtccca tttgttgacg gcaaccacca aggcgcggcc
     4741 tgcttccaaa gcaaaaccgg caatcgtcgc atcttggtcg gcgatgtcct gctgcgcgtc
     4801 caataccaaa acagcgacgt ttgccgcttc aaccgcctgc atcgctttga taacggagaa
     4861 tttttctact gcttcatcca ctttgccgcg acggcgcacg cctgcggtat cgatgatggt
     4921 aaacggtttg ccttcgcgtt cgaaatcgat gtggatgctg tcgcgtgtcg tgcctgccat
     4981 atcgaaagca atcacgcgct cttcgccgag gatggcgtta accagtgtgg atttgccgac
     5041 gttcggacgg ccgatgacgg caaagacggg atgttttgca tcggcttctt cggcttccgg
     5101 ctctgggaaa gtttccaaaa tatcttcaat cagataatac acgccatcgc cgtgcgcacc
     5161 tgaaataaca taagggtcgc ccaaagcaag ttcgtagaac tcggcggcaa gcacggccct
     5221 gttgccgcct tcgcctttgt ttaccgccag ataaacaggg cgcgggcttt ggcgcaggcg
     5281 gtcggcaatg attttgtctt gcggggtcag gccggtacgg ccgtccacca aaaacacaac
     5341 cgcatcagct tcatcgacag cctgcaaggt ttgttttgcc atttcgtgca aaatgccgct
     5401 gtccacaacc ggctcgaaac ctccggtatc gatgaccaaa taaggtttgc tgccgacttt
     5461 gccgtgtccg taatggcggt cgcgcgtcag accgggcagg tcgtgtacga gcgcgtcttt
     5521 ggtgcgcgtc aaacggttga ataaagtaga tttgccgacg ttggggcggc cgacaagcgc
     5581 gatggttggt ttcatgattg ggtctttctg tgtcaagtgc cgttcgggag aactgaacac
     5641 aagcaggtgt ccgttgggac acggcagatg gttttacggg aattgccgta ggatagtgtt
     5701 gtctgaaatg ccgtctgaag agagggtggc atttcagacg gcatttattt cagcgaatcg
     5761 agtttcattt gaaccaattc acgaccgaca gaatcttgag gcattttttc taaagcctga
     5821 ccgtagtttt ttaaggcttc ctggcttttt ccctgtgcgg catagacatc gcctttggtt
     5881 tccatcagca ggggggagaa gtccgcttca accggcgtgt cgagcgcggc aagtgcggca
     5941 tcgtattttt tttgttgcaa caacacaacg ccgagacgct gtgccgccaa cgcttgaatc
     6001 aggctgtctt tttggttgga caacacccat ttcaaatggc cttcagcaac atcgtaacgc
     6061 tgcgcgtcaa attcggttgc cgccgccatc agcgtggctt gggcggcgga aatggaatgc
     6121 gggtagcttt gttggagttt ggccaattcg gcattgattt cgttttgcgg ggctttgttt
     6181 tgcgcctttt ccacgatgtt tgccagcacc gccgccgctt cctgattttg ggaagccgca
     6241 cggttttggt aaaccgtgta tcccaagtag ccgagtgccg ccaaaatcag caaggcaaac
     6301 agccatttgc ccgtggtttt ccaaaaatat ttaaagttgt ctaactcttg ttgttcttcg
     6361 agatgggctg ccatttatgc gttcttccat tgttgtaaag tatgggttaa atcctcggcg
     6421 gcgacagttt gctgaccgtg tgcgccgttc atgtctttga gcgtaaccgt accgttcgcc
     6481 agttcgtctt gcgcgacaat cagggcaaag cgcgcgccgc tgttgtcggc ttttttcatt
     6541 tgtgctttca ggctttgata gccggaatgc tgcattacat tgaaaccttg cgtgcgcaag
     6601 gcttgcgcgt atttcatcac ctgcaagtcc gccctttcgc cttggtgcat tgcatagaca
     6661 tcaggcgcag cgttcacttc cagcgagccg tattcgctca ccaaaagcag cagccgttcg
     6721 atgcccatcg caaagccgat ggacggcgca ggtttgccgc caagttcctc aatcaaacca
     6781 tcgtaacggc cgccgccgca cacagttgcc tgcgcgccga gtttgtcggt cgtccactca
     6841 aaaaccgtct gattgtaata atccaaaccg cgaaccaagc gcggattttc aatatattga
     6901 atacccaaac catccaacat cgccttgaag cgtgcatagt ggttttgcga agcctcaccc
     6961 aagtaatcca ccagacgcgg cgcggcgttg cagatttcct gcaaatcagg gtttttcgta
     7021 tccaaaacgc gcaaaggatt ggttttcaga cggcgtttgc tgtcttcgtc caatttatct
     7081 tcataacggg tcagatattc aaccaatgcc gcacggtgtg ccgcgcgttc ctcacggttg
     7141 cccaaactgt tgatttccaa agtcagatat tcgcggatac ccaatttttc ccataagtcg
     7201 gcagacatcg cgatgatttc cgcatcaata tccggacctt caaaacccaa agcttcgata
     7261 ccgacctgat ggaactgacg ataacggcct ttttgcggac gctcgcggcg gaacatcggc
     7321 cccatatacc acagcttttg cgggctattg tacagaaggt tgtgttcgac caccgcacgc
     7381 agacaagaag ccgtaccttc aggacgcagg ctcaaactca aagaatcgtt tgaatcggag
     7441 aaggtgtaca tttccttgcc gaccacatcg gtttcttcgc cgatggagcg gacaaacaaa
     7501 ccggtttgct cgacaatcgg cgtacggatt tgctgataac cgtaagcgcg tgtccagcgg
     7561 ccgaccgtat cttcaaacgc ctgccaaaac gcagccgtca gtttgaaatc tttttgctcg
     7621 acaggcagaa ggtcgttcat gcctttgacg gattggattt tttgtgccat ttcaagtaag
     7681 aatgcttaaa tcaaattgcg ggcgattata gcggattttt aagggcttgt gaggatagag
     7741 atggtttgcg gacgggtaaa aatgccgtct gaaacagttt tcagacggca tttgacttac
     7801 tctgcacgtg cttgcctgat ttgtccgact gcaaactctg tctgccgctt gggatgatgt
     7861 gtgaaaaaca atttatttga aattgtctcg gcttttttcg gcacgaccgc caaaatctta
     7921 cctgccaaat ttccctcacg ggtttgccaa gcatccaaaa actgcgccct gctcattgaa
     7981 acatgcccca gcgacgggtc ggcaagcaaa accgtattgc cgtctatccc gcgcaatacc
     8041 gagaaatgat catccttgcg gtatttcaga tacacgatga cggggatttt caactgtgcg
     8101 agctgctcga aagacagggc atagcctttc gcttcaaaac ccaaatctgg cataatgcgc
     8161 cgcatatcct caaacgacgc gcgcatctgc tccttatcca gctttttcaa cacttcttct
     8221 tccgtcagcg tttgcccgta aaaattgttc aaaagcgtcg ccaccgaagc cgccccacag
     8281 gaaaaatcca aatcctgctt tacaatattg aaatcccgcc tttctttcca actctgcact
     8341 ttgatttttc cgtaaacaac aggattatcg ttaaacatcg gtgcagcatt caaacgataa
     8401 gataaagaaa cgacaacaca cgccaacaga aaaacatatt tgaacttcat catattgtcc
     8461 acataaaggg cagcctgaaa atctttcagg ctgcccttgt caaattattc ctagctttcg
     8521 gcttttttgg caaaccaaac aatccgatta cccgcataat actttccatt tattgaaatc
     8581 cgacaagccg cgcccaaaaa atgccatgca ctgtcgattt ccgcagcaat ctttgtaccg
     8641 ttttcttcaa attccaaata ttcacccaat aataaacttg aaacagaacg cgtgggcagc
     8701 aagtgcccac cctacgctta ttcaaataat ttgattaaat aaagaataaa gaaaatgcat
     8761 caggaacaaa attataatct gccacctgac tcacaccgct tttaaagtaa ggggcatcaa
     8821 aatcaaaacc gcaaaaaaaa taatttttgc attgattttt aatagattta aaattcgaat
     8881 atagtgtttc tccagattta aaaagtttat ctttatctat atatttgatg ctttccctat
     8941 ccaaaataaa tatttcaaac attaaaaaat cattacatga ccaagccaaa tcataaattt
     9001 gattcaaatg ggtatcataa agataaaaat aataaggttt gggaacaggt aaaatattta
     9061 atggaaaagg aggactaatt ttcttaatat ctgatgactg atatccatat ctttctatat
     9121 tcttaaaaat ttcatctttc ttaagataac aagacattct gacaatcaca acctgttcat
     9181 cggatatatt atctatttgc atggcgcgta taacacgcca tgcctgatta aaattagtct
     9241 cccttacctt agaatctatt atctttttca aagatgaaaa tgccccttat cttgaactct
     9301 ccagttagaa cctggaagtt tcgtacctct taaatgtgta ttaatatttc ttatagtttc
     9361 attaaaatgc cacgcgctgc cgatttcaac ggtaattttc gtaccgcttt cttcaaattc
     9421 caggtattcc cccattagct aacgcaaaga agcagacgcc atttcggctt cgttatgata
     9481 aacccgcctt ccgttgatat agacttccgc ccctgtccgc tccaaattcc aaaaattccg
     9541 tactgaaatt tccatatccc gatattgtgc agaccatgtt ttttcgaagg ttttcataaa
     9601 atttcctata cctgtccaat cggcacatat caattgcatt a
//
LOCUS       DAWFAN010000069         9512 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWFAN010000069 DAWFAN010000000
VERSION     DAWFAN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.069"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            134..343
                     /locus_tag="WGO65_001181"
     CDS             134..343
                     /locus_tag="WGO65_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ0327475.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            432..1646
                     /locus_tag="WGO65_001182"
     CDS             432..1646
                     /locus_tag="WGO65_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ0327476.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1733..4312)
                     /gene="clpB"
                     /locus_tag="WGO65_001183"
     CDS             complement(1733..4312)
                     /gene="clpB"
                     /locus_tag="WGO65_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ0327477.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLSSLPKVSGQGGDIMPSRELQAVLNLMDKAATKRG
                     DVYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4572..5582
                     /gene="trpS"
                     /locus_tag="WGO65_001184"
     CDS             4572..5582
                     /gene="trpS"
                     /locus_tag="WGO65_001184"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ0327478.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANSENNQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAAKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5650..6195
                     /locus_tag="WGO65_001185"
     CDS             5650..6195
                     /locus_tag="WGO65_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327479.1"
                     /translation="MLKTSFAVLGGCLLLAACGKSENTAEQPQNAVQSAPKPVFKVKY
                     IDNTAIAGLDLGQSSEGKTNDGKKQISYPIKGLPEQNVVRLTGKHTNDLEAVVGKCME
                     TDGKGAPSGWAANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            6514..6837
                     /locus_tag="WGO65_001186"
     CDS             6514..6837
                     /locus_tag="WGO65_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327480.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(7046..8554)
                     /gene="ppx"
                     /locus_tag="WGO65_001187"
     CDS             complement(7046..8554)
                     /gene="ppx"
                     /locus_tag="WGO65_001187"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ0327481.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALSERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(8671..9402)
                     /locus_tag="WGO65_001188"
     CDS             complement(8671..9402)
                     /locus_tag="WGO65_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0327482.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLRLSLGYKNR
                     SVTRSFGIVPFIGQNLLGGSRYNFVGGFNADFSRRLSERWRLTLNVGNMWKRYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHDLTKEAEQASIRKGLRVGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTLVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNMQIFMSVEKDFK"
BASE COUNT         2213 a         2473 c         2492 g         2334 t
ORIGIN      
        1 tgcggcttcg tcgccttgtc ctgatttttg ttaatccgct atatcgattt gaatttttca
       61 gaaaatgaag cggacgtttg ggcggaggca acttgtttga taagatagca atatttcaaa
      121 acggagaaag atcatgcctt atgtcaatat taaagtaacc ggcggcaagg aagcaccgac
      181 tgccgcgcaa aaagcggaac tgatcggcgg cgtaaccgaa ttgctggcac gcgtgctggg
      241 caaaaatccc gaaacaacgg ttgtcgtgat tgatgaagtg gataccgata actggggaat
      301 aggcggcaaa agcgtcagcg aacggcgcaa agagggcagg taaaaagcct gaaaatctcg
      361 gtttgatgct ttaaattccg cgtgaaaaag agtacattcc cacccattgc ccaaaattta
      421 cggaacacat catggataaa tttcccaagt ctgcaaagct cgatcacgtc tgttacgaca
      481 tacgcgggcc ggttcacaaa aaagcccttc agttggaaga ggagggcaat aaaatcctta
      541 aactcaatat cggcaaccct gcgccgttcg gctttgaagc ccctgatgaa atcttggtcg
      601 atgtcatccg caacctgccg acttcgcaag gctattgcga ttctaaaggg ctgtattccg
      661 cccgcaaagc cattgttcac tactatcaga ccaaaggttt gcgcgatatt acggttgatg
      721 atgtctatat cggcaacggc gtgtccgagc tgattacgat gtctatgcag gcattgctca
      781 acgacggcga cgaaatcctg attcccgcgc ccgactaccc tttgtggacg gcggcggcaa
      841 cgcttgcggg cggtacggta cgccattatc tgtgcgacga agaaaacggc tggttcccca
      901 accttgccga tatggaagcc aaaatcacgc ccaaaaccaa agccatcgtc gtcatcaatc
      961 ccaataatcc gacaggcgcg gtgtacagca gggaaatcct gttggaaatc gccgaactgg
     1021 cgcgcaagca cggtttgatt attttcgccg acgagattta cgataaaatc ctttatgacg
     1081 gcgcggttca ccaccacatc gccgcgcttg cccccgacct tttgacggta acgttcaacg
     1141 gtttgtccaa atcctaccgc gtcgccggct tccgccaagg ctggatggtg ctcaacgggc
     1201 cgaaacatca cgcaaaaggt tacatcgagg gtttggatat gctctcgtct atgcgcctgt
     1261 gtgccaatac gccgatgcag cacgcgattc agacggcatt gggcggctat cagagcatca
     1321 acgaatttat tttgcccggc gggcggcttt tggaacagcg caacagggcg tgggaactgg
     1381 tcagccaaat ccccggcgtg tcctgcgtca aaccgatggg cgcgatgtat atgttcccaa
     1441 aaatcgatac cgaaatgtac cgtatccacg atgacatgaa attcgtttac gatttgctgg
     1501 tgcgcgaaaa agtcttgctg gtacagggaa cggggtttaa ttggattaaa cctgaccatt
     1561 tccgcattgt cacgctgcct tacgtccatc agattgaaga ggcgatgggc aggttggcaa
     1621 gattcctgca aacctaccgc caataagggg acggtttgtc tgccgaggat aaaaaatgcc
     1681 gtctgaaacg gagattcccg tttcagacgg cattttcaac agcaggaacg aatcaggcaa
     1741 atttcagtct gtcgccgtcg gcttctaccc tgatttcgct ttcgggcgca tagtttccgg
     1801 caagcagggc ttttgccagc gggttttcga tttccgactg gatggcgcgt ttgagcggac
     1861 gtgcgccgta aatcgggtcg aaaccggctt tggcgatgat gtccagtgcg gcatcggaaa
     1921 cagtcaggcg caggttttgt ttttccaaac gtttttccaa acctttgagc tggattttcg
     1981 caatgctgcg gatgtttgcc tgatccaaac cgtggaacac gaccacttcg tcgatgcggt
     2041 tgatcatttc aggacggaaa tgttctttca catcctccat cacaacttct tttaccgctt
     2101 cgtaatcctg aatgcccatt tgttggatgt gctggctgcc gatgttggaa gtcatcacaa
     2161 tcacggtatt tttaaaatct accgtcctgc cttgcccgtc ggtcaaacga ccgtcatcca
     2221 atacttgcag caggatgttg aacacatcgg gatgggcttt ttccacttcg tccagcagaa
     2281 tcacgctgta cggtttgcgg cgcacttgtt cggtcaggta gccgccttct tcgtagccga
     2341 catagcccgg aggcgcaccg atcaggcgtg caacggcgtg tttttccata tattcggaca
     2401 tatcgatgcg aatcagatga tcttcgctgt cgaacagaaa gcctgccagg gctttacaca
     2461 actcggtttt acccacgccg gtcgggccca agaacaggaa gctgccgtaa ggcttgttcg
     2521 gatcggcaag accggagcgg ctgcggcgga tagcgtcgga cacggcacgc acggcttcgt
     2581 cctgtccgac cacgcggcgg tgcaatactt cttccatttt cagcagtttg tcgcgttcgc
     2641 cttccatcat tttggatacg ggaatgccgg tcatacggga aaccacctct gcgatttcct
     2701 ctgcgccgac attattacgc aagagtttgt ttgccggttt tgtgctgtcc gtatctgccc
     2761 gttcggcggc tgcacgctgt ttttccaaat gctccaaatc ttcatacatc aattttgaag
     2821 ccagtgccaa atcgccttgc cgttttgcct gttcgatttt aattttgact tcgtcaattt
     2881 gtttcttaat attagcagca ccgtctgaaa ttgctttttc ggctttccag atttcgtcta
     2941 aatcggcgta ttctttttgc agaccgttga tttcctcgtc tatcagttcc aaacgttttt
     3001 tgctggcatc gtctttttct ttttcaacgt gcgccttttc catccgaagc tgaattagac
     3061 ggcggtcgat tttgtccatt gcttccggct tggtttcttt ttccatcttg acacggctgg
     3121 cggcttcgtc aatcaaatca atcgctttat cgggcaggaa gcggtcggtg atgtagcggt
     3181 cgctcaactc cgctgcggca acgatagcag ggtcggtaat atcgataccg tggtggattt
     3241 cataacgctc ctgcaaaccg cgcaagatgg cgatggtgtc ttccacgctg ggctcgccaa
     3301 ccaatacttt ttggaaacgg cgttcgagtg ccgcgtcttt ttcgatgtat tggcggtatt
     3361 cgtccaaggt ggtcgcaccg atacagtgca gttcgccgcg tgccaaagcc ggtttcaaca
     3421 tattgcccgc gtccatcgcg ccgtcggttt tacctgcgcc aaccaaggta tgaatttcgt
     3481 cgatgaaaat cagagtgttg ccatcgtctt tcgccaagtc gttcaacacg cctttcaagc
     3541 gttcttcaaa ttcgccgcga tatttcgcgc cggcaatcaa agccgccaag tccagaacca
     3601 gcaggcgttt gttgcgcagg gattcaggca cttcgccgtt gacgatacgt tgcgccaagc
     3661 cttcaacaat agcggtttta cccacacccg gctcaccaat cagcacgggg ttgtttttgg
     3721 tacggcgttg caatacctga atcgcgcggc ggatttcatc atcacggccg ataacggggt
     3781 caagtttgcc gtcgcgggcg cgctgggtca gatcaagtgt gtattttttc aaagcatcgc
     3841 gttggtcttc ggcattcgca tcattcacgt tttgtcctcc tcgtaccgca tcaatcgcgg
     3901 cattaatgtt ttgttcggtc gcaccggctt ctttcaaaat tttgccggtt gcatcgttct
     3961 gctgcaccaa ggcaagcagg aaaagttcgc tggcgatata gacatcgccg cgcttggttg
     4021 cggctttgtc catcaagttc aatacagctt gcagctcacg gctaggcata atatcgccgc
     4081 cctggccgga cactttcggc aggctgctca aatgctgctg caaacgctgt ttcacctgcg
     4141 gcacgttcac gcccgaatgc gccagcaacg cggcggcacc gctgttttgg tcgtcaagca
     4201 gggcttttaa cacaaagcct gcttccagat agctgccgtc cgcagccaac gccaaactct
     4261 gagcttctgc aagggcttgt tggaatttgg cggttaattt gtcgtatcgc atttttgttt
     4321 ccttttcaaa atgtccgctg tcgaagccta tatgtgcata attgtggata actcaagttc
     4381 tgttttctgt ttttctatat ttaattcgat atatcattga atttaaagta tataaaaatg
     4441 tataataatg tgtataacta tatcttctta atatggaaaa gtctgttgtc ggctggatgt
     4501 aggtggcaaa tcgggtataa tcggcacatc tttttccctt tcagacggca ttgatgccgc
     4561 aaggacattt tatgagcaaa aaacgagttc tgaccggcgt aaccaccacc ggcatcccgc
     4621 atctgggcaa ctacgtcggt gctatccgcc ccgccgtccg cgcggcgcaa aaccctgata
     4681 ccgaatcctt cctcttcctc gccgattacc acggtatcat caaatgccac gagccggaga
     4741 tgattcacca atccacccaa gccgttgccg ccacttggct tgcctgcgga ctcgaccccg
     4801 agcgcaccac cttctaccgc caaagcgaca ttcccgaagt gatggaattg aactggattc
     4861 tgacctgcat caccgccaag ggtttgatga accgagccca cgcctacaaa gccgccgtgc
     4921 aggcaaattc agaaaacaat caggaagatc ccgatttcgg cgtggaaatg ggtttgttca
     4981 gctatccgat tctgatgacc gccgacatcc ttatgttcaa cgccaacgaa gtgcccgtcg
     5041 ggcgcgacca aatccaacac gtcgaaatgg cgcgcgacat cgccggccgc ttcaaccacc
     5101 gctttaaaga aatctttacc ctgcccgaag tgaaaatcga tgaaaacgtc gaactcttgg
     5161 tcggtttgga cggacgcaaa atgtccaaat cctacggcaa caccattccg ctttgggaaa
     5221 acgacaaaaa aacccaaaaa tcggtcaaca aaatcatcac caatatgaaa gagccgggcg
     5281 agccgaaaca gcccgacgaa agcccattgt ttgaaatcta caaagccttc tccacgccgt
     5341 ctgaaaccgc agcgttcacg caaatgcttg ccgaaggctt ggcttggggc gaggcaaaaa
     5401 aacttttggc ggcgaaaatc aacgccgaac tcgccgaacc gcgcgaacgc tacaacgagc
     5461 tgaccgccga cccttcgcaa atcgaagaga ttttgcaggc aggcgcggcg aaagcgcgta
     5521 aagaagcacg cgaattgttg gacaaagtac gcgatgcggt cggcatccgc ccgttgaaat
     5581 aaatccatgc cgtctgaaca tccattgtcc gtgtttcaga cggcattttg aaaccatcag
     5641 gagtgtggaa tgctgaaaac atcttttgcc gtattgggcg gctgcctgct gcttgccgcc
     5701 tgcggcaaat ccgaaaatac ggcggaacag ccgcaaaacg cggtacaaag cgcgccgaaa
     5761 ccggttttca aagtcaaata tatcgacaat acggcgattg ccggtttgga tttgggacaa
     5821 agcagcgaag gcaaaaccaa cgacggcaaa aaacaaatca gttatccgat taaaggcttg
     5881 ccggaacaaa acgtcgtccg gctgaccggc aaacatacca acgacttgga agccgtcgtc
     5941 ggcaaatgta tggaaaccga cggaaagggc gcgccttcgg gctgggcggc aaacggcgtg
     6001 tgccatacct tgtttgccaa actggtgggc aatatcgccg aagacggcgg caaactgacg
     6061 gattacctga tttcgcattc cgccctgcaa ccctatcagg caggcaaaag cggctatgcc
     6121 gccgtgcaga acggacgcta tgtgctggaa atcgacagcg aaggggcgtt ttatttccgc
     6181 cgccgccatt attgaggtat tcggacatcc cggaatatat ttgggttttt caaaccctgc
     6241 aggaaaaagt ccgcaccgtc gggaaactca aaaggagggg gatatgtgtt acaattttcc
     6301 gaactgtttt cataaaatag ttttcggacg tgtttcaata tggcattgat gccgccttat
     6361 tgttcgagaa aaaaccttta tatttaaata taatgggttt taactaaacg ggaaaccgtt
     6421 ttctctccgg ccgatgggca aaatcagccg attgatggaa cacggtccga tttttaagca
     6481 aaaccttaat caacaactca agaaggataa gacatgaaaa aattattgat tgccgcaatg
     6541 atggcggctg ccttggcagc ttgttcgcaa gaagccaaac aggaggttaa ggaagcggtt
     6601 caagccgttg agtccgatgt taaagacact gcggcttctg ccgccgagtc tgccgcttct
     6661 gccgtcgaag aagcgaaaga ccaagtcaaa gatgctgcgg ctgatgcaaa ggcaagtgcc
     6721 gaggaagctg taactgaagc caaagaagct gtaactgaag cagctaaaga tactttgaac
     6781 aaagctgccg acgcgactca ggaagcggca gacaaaatga aagatgccgc caaataattt
     6841 gttgccttgg caaaaatgat gggatgccgc ctgccggcaa cccaaacgaa accgcctgaa
     6901 gattttcagg cggtttttga gttgtatgcc ccttgttttt tacgcctgca tgaccgttcc
     6961 gagcagatgt gccgcgcata tcgggatgtt gcggacaggc aaatgccgtc tggacagggt
     7021 ttggacggca tttgttccgt aaggttcaga cgacctcgac tttaaacggc atattgattt
     7081 tttgccattg gacgctttca tattccaatg cgtcggcaat caggggatgg cgttccagcc
     7141 attccctgtc aatacgcagg atgaagccgc agctttccgt atccgtgcgc aactgcatat
     7201 ttttcgggaa agacaggtct tggcgcgaac ggcagaacag ggcagcaagg cgcagggaca
     7261 aaacggcata ccacaacatt tcgttggtgc cgatgatgcc gctcattttt ttcatatcgc
     7321 cgcgatgacc gatgaccagt tgggcaagta tggtctgttc tttgcgtgag aaacccggca
     7381 tatcggcgtt ttcgaggatg taggcggaat gcttgtgata gccggtgtgg gcgatgtcca
     7441 aaccgatttc gtgcagcgcg gcggcgcgtc cgagatactg ttgccacaag gcaagctctt
     7501 gaactgtaac gtttttagcg tggcagaggc tgtccataaa ggtttgcgcg gtctcggcgg
     7561 tgcgtttcgc ctgattgagg ctgacgtggt agcggtgttg gaactcggca accgtttgtc
     7621 cgcgcatatc ttcgtttaaa ccgcgcccga tcaaatcgta aaacacgccg tcgcgcaggg
     7681 cggcttcggt tacggtcatc ctgtcgagtt tcatttcctc aaacgccgcc atcatcacgg
     7741 caagtccgcc ggcaaaaact tcgatgcgtt ccggtttcag gttttcaaat ttggcttttt
     7801 tgaccgaacc ggcttcgatg atgcgttcgg agagggcgcg catacctttg taggtaatgt
     7861 ccgcctcttg gggcatttcg gcggcaagca cgtcgcggat ggattttgcc gaacccgatg
     7921 tgccgacggc gaaatcccag ccttcgcgcc tcatattttt gctgatgcgc tggatttcgt
     7981 tgcgggcggc ggaaatggcg gcttggaagt ctttggcggt gattttgttt tggaagaagc
     8041 gcaggctgta ggtaacgcag cccaagggca ggctttcggt aatgtcggga ttcagcgtcg
     8101 aaccgatgac aaattctgtc gaaccgcccc cgatgtcgat aaccagcatt ttgccgccgc
     8161 ccggggggag ggtgtggatc acgccggtat aaatcagccg cgcctcttcg cgcccggcga
     8221 tgatttcgat ggggaaaccc agtgccgctt cggctttggg aaggaaatct gcgatgtttt
     8281 tggcgacacg gaatgtattg gttgccacgg cgcgtacctg ttcagggcgg aagccgcgca
     8341 ggcgttcgcc gaattttgcc agacagtcca aagcctgttc ttgggaagcg gcactcaggt
     8401 ttttctgttc gtccagaccg gcggcgaagc gcaccatctg tttgaacgaa tcgataactt
     8461 tcaactgtcc gttgttgttt tcgcaaatct ggaggcggaa actgttggaa cccaaatcga
     8521 cggaggcgag gatgtttgcg ggggtggtgg tcatggcgga tatcggtggg ggaaaaacgc
     8581 aatgttactc tgacggcgca ggcgttgaca ataaatgatg cggctgtttt tgattctgct
     8641 cgcggatgtt gccgacggca ttttttgcgc ttatttgaaa tccttttcca cgctcatgaa
     8701 aatctgcatg tttttgcgtg tgtaaaaact tttcatattg ctgtcgattt tcagatagcg
     8761 gaaattgagt tgcggcgtaa agcccttcca agagattttg tcgtgccaca acgacaggtt
     8821 tgcctgatat tcgtggtctt tgcgcgggaa gcggtacaca agggtcccgg gtgcatcaaa
     8881 agtcctgcgg gtgtagcgca ggtttgcccg cagacccaag ccgccgtcga acgttttgac
     8941 cgctccgaca cgcaaaccct tgcggatgga agcctgttcc gcctccttcg tcaagtcgtg
     9001 cgaccagtcc gcaccgccgt aaagcagcca gtctttcggc gcggaataca tcagcgtcgc
     9061 gcccgccagc ggcatatggc tgtcgtatcg ggcggcggtg cggtcttcct gataacgctt
     9121 ccacatattg cccacgttca gcgtcagccg ccagcgttcg ctcaagcgtc gggagaaatc
     9181 ggcattgaag ccgccgacga aattgtatcg gctgccgcct aagaggtttt gcccgataaa
     9241 cggcacgatg ccgaacgagc gcgttaccga acgattttta tagccgagcg acaggcgcag
     9301 gcactgttcg ctgaaatctt tgttatccca ataatgcacg ccgccgccgc tgatgccgcc
     9361 gtagaggaaa tgatgcccgc ccgcattgat ttcgcgcgac acgcctaagc cctagcgcaa
     9421 accgtgtgcc ttttgcggca ggctgtcggc ggttttcgtc cagcttctgc ccgcaaattc
     9481 aatcgttttt ttggacgaag cgttgtttat ag
//
LOCUS       DAWFAN010000070         9417 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWFAN010000070 DAWFAN010000000
VERSION     DAWFAN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.070"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(115..1557)
                     /gene="aldA"
                     /locus_tag="WGO65_001189"
     CDS             complement(115..1557)
                     /gene="aldA"
                     /locus_tag="WGO65_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ0327483.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVST
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1592..1792)
                     /locus_tag="WGO65_001190"
     CDS             complement(1592..1792)
                     /locus_tag="WGO65_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327484.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVIYLGIN"
     gene            complement(1801..2190)
                     /locus_tag="WGO65_001191"
     CDS             complement(1801..2190)
                     /locus_tag="WGO65_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327485.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2398..3315)
                     /locus_tag="WGO65_001192"
     CDS             complement(2398..3315)
                     /locus_tag="WGO65_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0327486.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRRRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWNLSPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFNRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3487..4287
                     /locus_tag="WGO65_001193"
     CDS             3487..4287
                     /locus_tag="WGO65_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0327487.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4335..5111
                     /gene="mlaE"
                     /locus_tag="WGO65_001194"
     CDS             4335..5111
                     /gene="mlaE"
                     /locus_tag="WGO65_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ0327488.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVSGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5162..5656
                     /gene="mlaD"
                     /locus_tag="WGO65_001195"
     CDS             5162..5656
                     /gene="mlaD"
                     /locus_tag="WGO65_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ0327489.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGNSGKTYTVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5693..6283
                     /locus_tag="WGO65_001196"
     CDS             5693..6283
                     /locus_tag="WGO65_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ0327490.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6340..6618
                     /locus_tag="WGO65_001197"
     CDS             6340..6618
                     /locus_tag="WGO65_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ0327491.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6615..7442
                     /locus_tag="WGO65_001198"
     CDS             6615..7442
                     /locus_tag="WGO65_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ0327492.1"
                     /translation="MKKTAYAALLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7567..7929
                     /locus_tag="WGO65_001199"
     CDS             7567..7929
                     /locus_tag="WGO65_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ0327493.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7926..8312
                     /locus_tag="WGO65_001200"
     CDS             7926..8312
                     /locus_tag="WGO65_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0327494.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8309..8797
                     /locus_tag="WGO65_001201"
     CDS             8309..8797
                     /locus_tag="WGO65_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0327495.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKMANYYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8880..9047)
                     /locus_tag="WGO65_001202"
     CDS             complement(8880..9047)
                     /locus_tag="WGO65_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0327496.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9014..9394)
                     /locus_tag="WGO65_001203"
     CDS             complement(9014..9394)
                     /locus_tag="WGO65_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ0327497.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
BASE COUNT         2179 a         2625 c         2369 g         2244 t
ORIGIN      
        1 aaagacaaaa acatcgatgt cggtcatgac tgattgccgg tgaaactgta aaaatgccgt
       61 ctgaacctga aaacgggttc agacggcatt ttctatcggg gttttaaagc ggcattaaat
      121 gtcggtttcc aaataaacga cttgggtttg cagatattct tccaaaccgt gtttgccgtc
      181 cgcgccgccg ataccggatt ttttccaacc ggcgtggaaa ccctgcatcg cttcaaagtt
      241 ttcgcggttg atgtaggttt cgccgaattg caggcggcgg gtaacgtaga aggcttcgtt
      301 taaattagtc gtataaacag aactggtcag accaaactcg caatcgttag ccagtgcgat
      361 gacttggtcg agcgtgtcga aagtggaaac aggcaggacg gggccgaagg tttcttcttt
      421 cataatgtcc atactgttgt cggtgtcggt cagcagggtc ggctcgaaga aatagccgcg
      481 tccttcggcg cgtttgccgc cgcaaaccaa tttcgcacct tgtttgactg cccgttccac
      541 tttttcggca acggctttga cggcgcgctc ttcaatcagc gggcccattt ccagcgcgcc
      601 ggcttcggct tcggcagggt tgccgtagcg cacgcctttc atcgcggcgg tcattttttc
      661 gataaacgcg tctttcaggc tgctgtggac atagacgcgc tcggcgcagt tgcagatttg
      721 accagtgttg ccgacgcgcg aagccaagat ggatttcacc gccaaatcca aatccgcgtc
      781 tttcaaaacg atggcggggg ctttgccgcc gagttccagc gaaactttgg tgatgttggc
      841 ggaagcggct tccatcactt ggcggcctgc ttcgacggag ccggtcaggc tgaccatatc
      901 gacttgcgga tgggcagaca aggcattgcc gatttccgca cccggaccgt tcaccacgtt
      961 gaacacgcct gcgggcaatc cgaccgcatc gacgatttcg gcgaagatgt ggcagttgat
     1021 cggggttacg ctgctgggtt tgacaacgat ggtgttgccc gttactaagg cgggacccat
     1081 tttgcgggca atcaggaaga aagggaagtt ccacggcaaa atgccggcaa ttacgcccag
     1141 cggacgtttg aacaataaaa tattttcacg cgggcggtcg ctttggatga tttcgccttc
     1201 gtaacggcgc gcccattcgg cttggtaatc gagatagtcg gcggtgaaca tcacttctac
     1261 gcgcgccaag tctttggttt tgccgccttc ggcaacgatg gtgtcggtca gctcgtcggc
     1321 gcgtccgcgt atgccttggg cgattttgcg caaatacgcg ccgcgttcga ccgcaggcag
     1381 gcgttcccaa gccggttgcg ccgcgcgtgc cgccgccaca gctcggtcaa cgtctgcttt
     1441 tccgcctttg ggttcgcggg cgatggtttc ttcggtggaa gggttcaata cgtcgcgcca
     1501 ttcgccgttg aagtcgtttt caaagcgtcc gttgatgtac atggccaatt gtttcatttc
     1561 gggttctcca gttttgtagt cagatgtagt tttagtttat tcccaaataa attaccgtac
     1621 aagtttcttt tgaaatgttt agacgagaag ctactatgtc tgcatattgt tctgctaatt
     1681 tatattcact cgaatgcaac tgccgattat tccaatctgc attcgcatca gtaaaagccc
     1741 cagcattgcc gcctgtggca taaccaatcg ctaaactgcc aacggtatcc acaacaggtt
     1801 tcaggctgcc tgaatttctc taataaatga aacacttatt ctatcagccg ttaatgtaac
     1861 caaacaatta tcaagataag agtagtttat taaatactca tcttcttcaa attgtatatt
     1921 ttgtagggtt agatgattat tttttaattc tgccgttatt tcatacttga aactatcatc
     1981 tatttttcta aatccaatat ttgaagttga tttatccagt atgtaatcat taaagatttc
     2041 ataggataat tccacttcat aaaattgatt ttcaaatatt ttgaatgggc agtacgatat
     2101 aaaacaatgt agttcataac catttatttc aatcaatact gcctcttcaa tggtgttatc
     2161 taactttttg attttacatt tatataacat ttatcttgcc tttggataaa ttgttttgtt
     2221 ttttggattg ttattcaaag ttctccaaaa gtcttttcct gattttgtat gtatcgtggt
     2281 aatactacca ttttcattgg ttccaacaaa aataaattta gattgacctt tggaatcagt
     2341 acccaaaaac tgaacatatc cagacatgcg gatttcgggt tttaaatcaa atgcggctta
     2401 tcggttttct tttttggttt ccgcaggcgt gctgccgaaa tattgcctga atgcctgagt
     2461 aaagctggaa acgtggcggt agccgcacaa atacgcggtt tcgcctatgc ttttgccccc
     2521 gttttgcaac aggtagagcg cgtgctgcat ttgtttgtgg tgcagccact cgcttgcggt
     2581 aataccgaaa tggtcgcgca tacggcgttg cagcgtcctt tcactgatgt tcagcgcgtc
     2641 tgtcagccgg ttgacttggt gtgcgccgtc ggcaaacgcg gcatttaggg tgcggttgaa
     2701 gtcttcagac ggcatagcgt ctgcttccgc cgtttgcccc gccgccggcc cgatgccgtc
     2761 tgaaaccgtg tcccacaagt ccgacagcag ccgcaaaacg tccgcctcgc ggcgcaatgt
     2821 ttcgcccaaa tgcccctttg ggacagcttt caggcaggat gccgccaagc cgcgcaggtt
     2881 tggggacaaa ttccatattc tgaccggttc gcggtaaagc aggggtgcga aacgcgcata
     2941 ctccggacgc agcagccatt gttccatacc tttaatggtc attttgaccg ttttgccgcc
     3001 tcggtaaaga tagcggctga acaggacctc ttccccgaca gcaattagga cgatcttgcc
     3061 gccatccgca tcgatttgga agcggcggcg gttgatgccg aagtccaaac tgccttccag
     3121 caagagcacg aacgacacat aaggttcggc gaggcggctg ctgcaaaaat cgcaccgtgc
     3181 cgttaccgtg ccgccgtgga tggaaatgcc gttggacaaa gtatcgaaac ggtagctgcc
     3241 ctccacatag ctgccgccgc cgcttttgac cagattgacg aaccggctgc tgttcaaatg
     3301 ttctgctttt tccatagttt tctgataaag gttatcattt gaaagataac atttttcgcc
     3361 acagcaaaca atccgccgcc acaacggcat tttcacaaca tttaccgtcc gtatgccgtc
     3421 tgaaaattgc ccgtcttccc gataagcctt aaaatacacg ccgtcaaacc taccgtccgt
     3481 ccgtacatga gcccatcccc ctttatcgaa atgaaagacg tcgccttcgc gtatggcgac
     3541 cgcccgattc tgaagaacat caatttcagc attccgcaag gcaattttgc tgccgtaatg
     3601 ggcggttcgg gcagcggcaa aaccacgctg atgcgactga ttacaggaca gattcgtccg
     3661 cagtccgggc aggttttgat tgaaggacgg gatttggcgg gcttttcggc tgacgagttg
     3721 tatgaacacc gccgccgtat gggcgtattg ttccaacacg gcgcgctgtt taccgatttg
     3781 tctgtatttg acaatattgc ttttccgatg cgcgaactga cgcgcctgcc ggaagcggtg
     3841 attcgagatt tggttttgtt gaaactgaac gcggtcgggc tgcgcggcgt ggaaaacctg
     3901 atgccgtccg agttgtccgg cgggatgtcg cgccgcgtcg cgcttgcccg cacaatcgcg
     3961 ctcgatcccg aaattatgtt gtacgacgag ccgtttaccg gtctcgatcc gatttccttg
     4021 ggcgtgattg cccacttgat cagccgcgtc aacaaggctt tgcgttcgac cagtattatg
     4081 gtcacgcacg acattgaaaa atctttggaa atcgtcgatc aggtgatttt cttggcgcac
     4141 ggcgaaatta tgttctccgg ctcgccgcag gaaatgcgcg aactggattc gccttgggtg
     4201 cgccagtttg tcggcgggct ggcagacggc cccgtggcat accgttatcc ggcgcaaacg
     4261 tcgttgcagc aggacctgtt gggataaatg agttgatgcc gtctgaaacc gttgaacttt
     4321 acacatagaa atttatgaac tttatccgtt ccgtcggggc gaaaaccctc ggccttattc
     4381 aatccctcgg cagtatcacg ctgtttctgc tgaacattct ggcgaagtcc ggtacggctt
     4441 tcgtccgtcc gcgcctgagc gtgcgccaag tgtatttcgc cggtgtgctg tcggtgttga
     4501 ttgttgccgt ttcggggctg tttgtcggca tggtctcggg tttacagggc tatacgcagt
     4561 tgtcgaaatt caaatccgcc gatattttgg gctatatggt cgcggcttcg ctattgcgcg
     4621 agctgggtcc ggtattggca gcgattttgt ttgccagcag cgcgggcggt gcgatgacca
     4681 gcgaaatcgg tctgatgaaa acgaccgaac agctcgaagc gatgaatgtg atggcggtaa
     4741 accctgtcgc ccgcgtggtt gcgccgcgct tttgggcggg cgtgttttcc atgccgcttt
     4801 tggcttcgat tttcaacgtg gcgggcattt tcggtgcgta tttggtcggt gtaagctggc
     4861 tgggcttgga cagcggcatt ttctggtcgc aaatgcagaa caacatcacg atacattacg
     4921 atgtaatcaa cggtctgatc aaatccgccg cgttcggcgt ggcggtaacg ctgattgccg
     4981 tgcatcaggg cttccactgc gtcccgacct cggaaggcat tttgcgcgcc agcacgcgca
     5041 cggtggtttc gtccgccctg acgattttgg cggtcgattt tatattgacc gcgtggatgt
     5101 ttacagattg acagggcgtc tgaagacgaa acgcggaaca tcggatattc aaggaacttt
     5161 aatgaaaaag aacatattgg aattttgggt cggactgttc gtcctgattg gcgcggcggc
     5221 ggttgccttt ctcgctttcc gcgtggcggg cggcgcggcg ttcggcaatt ccggcaagac
     5281 ttataccgta tatgccgatt tcggcgacat cggcggtttg aaggtcaatg cccccgtcaa
     5341 atccgcaggc gtattggtcg ggcgcgtcgg cgctatcgga cttgacccga aatcctatca
     5401 ggcgagggtg cgcctcgatt tggacggcaa gtatcagttc agcagcgacg tttccgcgca
     5461 aatcctgact tcgggacttt tgggcgagca gtacatcggg ctgcagcagg gcggcgacac
     5521 ggaaaacctt gctgccggcg acaccatctc cgtaaccagt tctgcaatgg ttttggaaaa
     5581 ccttatcggc aaattcatga cgagttttgc cgagaaaaat gccgacggcg gcaatgcgga
     5641 aaaagccgcc gaataaacgt atgccatcat attcaaacta ggacacttaa tcatgaaaaa
     5701 atcctccctc atcagcgcat tgggcatcgg tattttgagc atcggcatgg catttgccgc
     5761 ccctgccgac gcggtaaacc aaatccgtca aaacgccact caagtattga gcatcttaaa
     5821 aagcggtgat gccaacaccg cccgccaaaa agccgaagcc tatgcgattc cctatttcga
     5881 tttccaacgt atgaccgcat tggcggtcgg caacccttgg cgcaccgcgt ccgacgcgca
     5941 aaaacaagcg ttggccaaag aatttcaaac cctgctgatc cgcacctatt ccggcacgat
     6001 gctgaaatta aaaaacgcca acgtcaacgt caaagacaat cccatcgtca ataaaggcgg
     6061 caaagaaatc atcgtccgcg ccgaagtcgg cgtacctggg caaaaacccg tcaacatgga
     6121 cttcaccacc taccaaagcg gcggtaaata ccgtacctac aacgtcgcca tcgaaggcgc
     6181 gagcctggtt accgtgtacc gcaaccaatt cggcgaaatc atcaaagcga aaggcgtgga
     6241 cggactgatt gccgagttga aggctaaaaa cggcagcaag taatccataa gccatgccgc
     6301 cagactgaag atggcatgga tttttcccta ccgaacacta tgcatacaga actcaacaac
     6361 ggaacactgc acatcagcgg cgacgtaacc gtcaaaaccc tgactgccgc cgcctttacc
     6421 cgcttcagac agcaatgtcg tctgaaagac acccgcgcgg ttgatttgag cggcgtcgga
     6481 cgcgccgatt ccgcctgcgt gtcgctgctg ctcgaagtgc tgcgcggttg caaaggcagc
     6541 gtcaggctga ccggcattcc cgaatccgtg cgcgcgctgt ccgaactgta cgaaatcaaa
     6601 gactggttga aatcatgaaa aaaaccgcct acgccgccct cctgctgatc gggttcgctt
     6661 ccgcccctgc atttgcagaa acccgccccg ccgaccctta tgaaggctac aaccgcgccg
     6721 ttttcaaatt caacgaccaa gccgaccgct acattttcgc ccctgccgcg cgcggctacc
     6781 gcaaagttgc gccgaaaccc gtccgcgccg gcgtgtccaa tttttttaac aacctgtgcg
     6841 acgtggtcag cttcggcagc aatatcttgc gcttggacat caaacgcgca agcgaagacc
     6901 ttgtccgcgt cggcatcaac accactttcg gtttgggcgg gcttatcgac atcgccggcg
     6961 cgggcggcat tcccgacaat aaaaacacct tgggcgacac gtttgcttcg tggggatgga
     7021 aaaacagcaa ttatttcgtg ttgcccgtct tagggccgtc caccgtccgc gacgcgctcg
     7081 gcacgggtat tacctccgtt tattcgccca agaatatcgt cttccgcacc cctgtcggac
     7141 gctggggcac gactgccgta tccgccgtca gtacgcgcga aggtctgctc gatttgaccg
     7201 acagtctgga cgaagccgcc atcgacaaat acagctacac gcgcgacctc tatatgaaag
     7261 tccgtgcgcg gcagaccggt gcaacacctg ccgaaggtac ggaagataac atcgacatcg
     7321 acgaattggt cgaaagtgcc gaaaccggcg cggcggaaac tgccgttcaa gaagattccg
     7381 tatccgaaac acaggcagaa gcagcagggg aagccgaaac gcaacctgga acacaacctt
     7441 aaagccatgc cgtctgaaaa tccgtgttcg gacgacattc ccttctgtcg gaacggacag
     7501 tccgaaacca cagattgggc tataatctct tttttgcgca taccgcgcga cagcaaagga
     7561 aaaaccatgt acgaagtcaa ccgcagcgta ttcgtcctca tccctttaga acctttttgg
     7621 aactggctgc aaaccctgcc gggcaaccac ctcgacggac tgactcttga agacatccaa
     7681 gccgacgcca attcctacct tgtccgcccg tgcgaaaccg ccgacgaagt atgggacgaa
     7741 atcgaagccc gttttgaaga cattttcgcc gccgaacttg ccgactggtg cgaagacgaa
     7801 cgcgagtggc ctgcgctcga tgccgacatt ttcaacgaat ggttcgacat ccagctttct
     7861 accgtcatca ccgacctcga acacgaaccg cttgcccgcg aagccttcca acccatcaac
     7921 ctgaactgat gaagctgacc gtccgcaact accatctcga cggctacggc catgtcaaca
     7981 atgcgcgcta cctcgaattt ttcgaagaag cgcgctgggc gttttttgaa gaacgcggac
     8041 tgctgcacga gttggcaggc ctcatactga ttgtcgcccg catcgacatc cgatacagcc
     8101 gccccgccgt cgaaggcgac gtattgcagt tttcatgccg cctgaaaacc cccggcacgc
     8161 gccgtattgt gctgacccag acgattacac tgccaaacgg caaaaccgcc gcagaagccg
     8221 acatcaccct gatgcccgtc cacgccgcca cacaacgcac cgtcagcctg cccgccaccc
     8281 ttgcccgcgc attggaagcc ttgtccgaat gaaaaaaata ctcaccgccg ccgtcgtcgc
     8341 actgatcggc atcctccttg ccatcgtcct catccccgac agcaaaaccg cgcccgcctt
     8401 ctccctgccc gacctgcacg gaaaaaccgt ttccaacgcc gacctgcaag gcaaagtaac
     8461 cctgattaat ttttggtttc cctcctgtcc gggttgtgtg agcgaaatgc ccaaaatcat
     8521 taaaatggca aattactata aaaacaaaaa cttccaagtc cttgccgttg cacaacctat
     8581 cgatccgata gaaagcgtcc gccaatatgt caaagactac ggtttgccgt ttaccgtcat
     8641 gtatgatgcg gacaaagctg tcggacaggc gttcggcaca caggtttatc cgacttccgt
     8701 ccttatcggc aaaaaaggcg aaatcctcaa aacttatgtc ggcgaacccg atttcggcaa
     8761 actctaccaa gaaatcgata ccgcgctggc acaatagcga tgccgtctga agcgcaaaaa
     8821 aaacagaaac gatgttcctg ttttttcaga tcatccgccc cgacacgttc agacggcatt
     8881 caaccttccg gcagattctt tttcaccact gccggattgc ctgccgccaa agagtagggc
     8941 ggaatgtctt ttgtaaccac cgcgcctgcg cccacgaccg aaccgcgtcc gacggttacg
     9001 cccgccatta caatcaccct gtgccccggc cagacatcgt cctccaacgt aatcggacgg
     9061 atttccgtgt agccctcaaa gcgttttttg aacggtcaaa cttgtggtta tttgaataaa
     9121 acagacattc cggctccatc atcacatttt tgccgaccac cagcccacgg cagatttcac
     9181 agtttgcccc gatgcccgag ccgtcgccca aaaccgtatc cggaaacaca tacgccccgc
     9241 gttcgatatt gaccccgcgt ccgatatgcg gagaaacccg ccgcgccaaa aaaccgcgca
     9301 cccgtctgcc gacgatgccg atgccgcgca tatgtgacgg cggcaacccc gaaccggcaa
     9361 aacgcagcag caggaaaaag aattttgatc ccatcaaacc tccctcaaaa gaacttt
//
LOCUS       DAWFAN010000071         9321 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWFAN010000071 DAWFAN010000000
VERSION     DAWFAN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.071"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            164..1507
                     /locus_tag="WGO65_001204"
     CDS             164..1507
                     /locus_tag="WGO65_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ0327498.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1510..2742
                     /locus_tag="WGO65_001205"
     CDS             1510..2742
                     /locus_tag="WGO65_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ0327499.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2735..3511
                     /locus_tag="WGO65_001206"
     CDS             2735..3511
                     /locus_tag="WGO65_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ0327500.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3511..4137
                     /locus_tag="WGO65_001207"
     CDS             3511..4137
                     /locus_tag="WGO65_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ0327501.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTIFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4141..4734
                     /gene="nqrE"
                     /locus_tag="WGO65_001208"
     CDS             4141..4734
                     /gene="nqrE"
                     /locus_tag="WGO65_001208"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ0327502.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4748..5965
                     /gene="nqrF"
                     /locus_tag="WGO65_001209"
     CDS             4748..5965
                     /gene="nqrF"
                     /locus_tag="WGO65_001209"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ0327503.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMEIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6117..7172
                     /locus_tag="WGO65_001210"
     CDS             6117..7172
                     /locus_tag="WGO65_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0327504.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQTTADKPLRIS
                     SDFAHVTAEAIRLNRLTHGALDVTVGPLVNLWGFGPDKSVTQEPTPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            7198..7413
                     /gene="nqrM"
                     /locus_tag="WGO65_001211"
     CDS             7198..7413
                     /gene="nqrM"
                     /locus_tag="WGO65_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ0327505.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7537..8115)
                     /gene="grpE"
                     /locus_tag="WGO65_001212"
     CDS             complement(7537..8115)
                     /gene="grpE"
                     /locus_tag="WGO65_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ0327506.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAESAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8299..9117
                     /gene="cysE"
                     /locus_tag="WGO65_001213"
     CDS             8299..9117
                     /gene="cysE"
                     /locus_tag="WGO65_001213"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ0327507.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2260 a         2605 c         2312 g         2144 t
ORIGIN      
        1 aacggcttcc gcaaggaaaa aagcagcaaa tctgacaaac aggtacaatt cctgatagaa
       61 tctaacactt tgctacatta atttattcaa tgcttttgat gccacgcgac acggtatcaa
      121 aacacgccgt ttttacctta accatcacaa gtaatgcaaa tatatgatta aaatcaaaaa
      181 aggtctaaac ctgcccatcg cgggcagacc ggagcaagtc atttatgacg gcccggccat
      241 taccgaagtc gcgttgcttg gcgaagaata tgccggtatg cgcccctcga tgaaagtcaa
      301 ggaaggcgat gccgtcaaaa aaggccaagt gctgtttgaa gacaaaaaga atccgggcgt
      361 ggtgtttacc gcgccggttt caggcaaaat cgccgccatc catcgcggcg aaaagcgcgt
      421 acttcagtcg gtcgtgattg ccgttgaagg caacgacgaa atcgagttcg aacgctacgc
      481 gcccgaagcg ttggcaaact taagcggcga agaagtgcgc cgcaatctga tccaatccgg
      541 tttgtggact gcgctgcgta cccgtccgtt cagcaaaatc cctgccgtcg atgccgagcc
      601 gttcgccatc ttcgtcaatg cgatggacac caatccgctg gcggcagacc ctgtggttgt
      661 gatcaaagaa gccgccgagg atttcagacg aggtttgctg gtattgagcc gtttgaccga
      721 gcgtaaaatc catgtgtgta aggcagctgg cgcagacgtg ccgtctgaaa atgctgccaa
      781 catcgaaaca catgaattcg gcggcccgca tccggccggt ttgagtggca cgcacattca
      841 tttcattgag ccggtcggtg caaacaaaac cgtttggacc atcaattatc aagatgtaat
      901 tgccatcgga cgtttgtttg caacaggccg tctgaacacc gagcgcgtga ttgctttggg
      961 tggttctcaa gtcaacaaac cacgcctctt gcgtaccgtt ttgggtgcga aagtatcgca
     1021 aattactgcg ggcgaattgg ttgacgcaga caaccgcgtg atttccggtt cggtattgaa
     1081 cggcgcgatt acacaaggcg cgcacgatta tttgggacgc taccacaatc agatttccgt
     1141 tatcgaagaa ggccgcagca aagagctgtt cggctgggtt gcgccgcagc cggacaaata
     1201 ctccatcacg cgtacgaccc tcggccattt cctgaaaaac aaactcttca agttcacgac
     1261 agccgtcaac ggtggcgacc gcgccatggt gccgattggt acttacgagc gcgtaatgcc
     1321 gctagacatc ctgcctaccc tgcttttgcg cgatttaatc gtcggcgata ccgacagcgc
     1381 gcaggcattg ggttgcttgg aattggacga agaagacctc gctttgtgca gcttcgtctg
     1441 cccgggcaaa tacgaatacg gcccgctgtt gcgtaaggtg ctggaaacca ttgagaagga
     1501 aggctgatta tgggcttgaa acattttctg gaaaaaatcg aaccgcactt cctgccgggc
     1561 ggcaaacatg aaaaatggta tgccctctac gaagctgcgg cgacgatttt ctatacatcc
     1621 ggtgcggtaa cgcgcaaagc ggcacacgtc cgcgatgcgc tcgactccaa acgcatgatg
     1681 attttggtgt ggctggcttt gttccctgcc atgttctacg gtatgtacaa cgtcggcgca
     1741 caggcattcg gtgccttaac gcccgatttg ctgcaacaaa gcatcgccaa cgactggcat
     1801 tacgcccttg ccaacgcttt gggcatcaat atgtcgtctg aagcgggcgt gttgggcaaa
     1861 atgctgttcg gcgcgattta cttcctgccg atttacgcga ccgtatttat tgtcggcggt
     1921 ttctgggaag ttttgttcgc caccgtgcgc aaacatgaaa tcaacgaagg tttctttgtt
     1981 acctcgattc tgtttgcctt aatcgttccg cccacgctgc cgctgtggca ggcagctttg
     2041 ggtatttctt tcggcgttgt ggttgcgaaa gaagtattcg gcggtacagg taaaaacttc
     2101 atgaaccctg cgctggcagg ccgtgccttc ctgttcttcg cctaccctgc caacttgagc
     2161 ggcgatgcgg tttggacggc ggttgacggc tattccggcg caaccgcgct ggcgcaatgg
     2221 gcggcacacg gtgcagacgg cctgaaaaac gccgtaaccg gtcaaaccat cacttggatg
     2281 gatgcgttta tcggcaaact gcccggctcc atcggcgaag tctccacttt ggcactctta
     2341 atcggcggcg cgtttatcgt gtttgcccgc atcgcttctt ggcgcattat tgccggcgtg
     2401 atgatcggta tgattgccat gtcttcgctg ttcaacttca tcggttcgga caccaacgct
     2461 atgtttgcta tgccttggta ctggcatttg gtcgtcggcg gcttcgccat cggtatgctg
     2521 tttatggcga ccgaccccgt ttccgcttcc tttaccaatg tcggcaaatg gtggtacggc
     2581 gcactgatcg gcgtgatgtg cgtattgatc cgcgtggtca accctgctta ccccgaaggc
     2641 atgatgttgg cgattctgtt tgccaacctg tttgccccga ttttcgacta ttttgtcgca
     2701 caagcgaaca tcaaacgcag aaaggcgcgc agcaatggct aagaaattcg ataaagacag
     2761 cttcagcggc acgctgattg tggtgttggc ggtcagcctg atttgctcgg tcatcgttgc
     2821 cggtgcggtc gtcggcttga aacccatcca agagaaacaa aaactccaag acaaacaagg
     2881 ctatatcttg agcgtagccg gtttgatgga taaggacacc gacatcggta aaacctttgc
     2941 cgagcgtatc gagcaacgtg ttgtcgattt ggcgaccggc gaatacgtcg ccgatgcgcc
     3001 taaagacttc agcgcgcgca tcgcaggcaa agaccctgcc caaagcatcc gcatcaaacc
     3061 cgaagacgat ttggcaggca tcaaaagccg tgccaaatac accgaggttt atttggtaaa
     3121 aggcgaagac ggcaaaatcg ggcaaatcat cctgcctatg cacggtaacg gtttgtggtc
     3181 ggtcatgtac ggctttgtcg ccatccagcc cgacggcaac accatcaacg gcattaccta
     3241 ctacgaacaa ggcgaaaccc cgggcttggg cggcgaaatc ggcaatccgt tgtggcagca
     3301 aaaattcgtc ggcaaaaaac tgtttgacgg acaaggcaaa ctcgccctgc acgtcggcaa
     3361 aggcgcgggt tcggacaaag aacacggcgt agatgccctc tccggcgcgt cgctgacatc
     3421 caaaggcgtg caaggttcgt tcgcctactg gttcggcgaa aacggctata tcccctacct
     3481 gaacaaattg aaatcagcag gagcgcaata atggctgata tgaaacgctt gaaacactta
     3541 atgttttcac cctttatcga caacaacccg attgccttgc aggttttggg tatttgttcg
     3601 gctttggcgg ttaccaccaa acttcagacg gccatcgtga tgggtatttc cgtagctttg
     3661 gtaaccggtt tttccagctt cttcatttcg ctggtacgca actacatccc caacagcatc
     3721 cgcatcatcg tgcaaatggc aattatcgcg tcgctggtta cgctggtcga ccaactcttg
     3781 caggcatttg cctacgaatt gtccaaacag ctttccgtat tcgtcggtct gattattacc
     3841 aactgtatcg tgatgggccg cgccgaagca tttgcgatga aagagccgcc gctggaaagc
     3901 ctgatcgacg gtatcggcaa cggcgcgggc tacgggatga tgctgcttgt cgttgccacc
     3961 gtccgcgaac tgatcggctc gggcaaactc ttgggctaca ccattttcca aaccgtgcag
     4021 gacggcggct ggtatcagac caacggcttg ttcctgctcg cccccagcgc gttcttcatc
     4081 atcggctttt tgatttgggg actgcgtacc tggaaacccg aacaggcgga ggaataagct
     4141 atggaacact atttgagcct cttcatcaaa tccgtcttca ttgaaaatat ggcgctatcc
     4201 ttctttttgg gtatgtgcac gtttttggcg gtatccaaaa aagtatccac cgcattcggt
     4261 ttgggtgtgg cggtaatttt cgtactcggg ctgtccgtcc ctgtcaacca actcgtttac
     4321 tcgctgctca aagacggcgc gattgtcgaa ggcgtggatt tgaccttttt gaaattcatc
     4381 accttcatcg gcgtgattgc cgctttggtg cagattttgg aaatgttctt ggataaattc
     4441 gtccccgccc tctacaacgc attgggcatc tacctgccgc tgattaccgt gaactgcgcg
     4501 attttcggcg ccgtatcgtt tatggcgcaa cgcgaataca acttcggcga gtccgttgta
     4561 tacggcttcg gcgcgggctt gggctggatg ttggcgattg tcgctttggc gggcattacc
     4621 gaaaaaatga aatattcgga cgctcccaaa ggcctcaaag gcttgggcat tacctttatc
     4681 gccgccggcc tgatggcgat ggcgtttatg tcgttctccg gcatccagtt ataagaaagg
     4741 gatcggtatg gagattattt taggtatcgt gatgtttacc gtcatcgttt tggttttggc
     4801 actgatgatt ctgtttgcca aatccaaact ggtcagcgaa ggcgacatca ccatcaaagt
     4861 caacggcgaa aaagagctga cgatgcccgc cggcggcaaa ctcttgggcg cgcttgccaa
     4921 cgaaggcatc tttatcccct ccgcctgcgg cgggggcggt tcgtgcggac aatgccgcgt
     4981 cgtcgtaaaa agcggcggcg gcgacatcct gccgaccgag ctgtcccaca tcagcaaacg
     5041 cgaagcacgc gaaggctgcc ggctgtcgtg ccaagtcaac gtcaaaaccg atatggagat
     5101 tgaagtaccc gaagaagtgt tcggtgtgaa aaaatgggaa tgcaccgtca tctccaacga
     5161 caacaaagcc acgttcatta aagaactcaa gctcgccatt cccgaaggcg aagaagtccc
     5221 cttccgcgcc ggcggctaca tccaaatcga agccccgccg cacaccgttg cctacaaaga
     5281 ctttgacatt cctaaggaat atcacgaaga ctgggacaaa tacaatttgt ggcaatacgt
     5341 ttccaaagtg gacgagccga ttttgcgcgc ctactctatg gcttcgtatc ctgaagaaaa
     5401 aggcatcatt atgctgaacg tgcgtatcgc cacgcctccc ccgcgcgtac ctgacgcgcc
     5461 tccgggacaa atgtcgtcct acatctggtc gctcaaaccc ggcgacaaag ttacgatttc
     5521 cggcccgttc ggcgaatttt tcgccaaaga caccgatgcc gaaatggtat ttatcggcgg
     5581 cggtgcgggt atggctccga tgcgttccca cattttcgac cagttgaaac gtttgaactc
     5641 caaacgtaag attaccttct ggtacggcgc acgttccaaa cgcgagatgt tctatgtcga
     5701 agactttgac caactcgcgg cagagttccc gaacttcacg tggcacgtcg ccctgtccga
     5761 cccgctgccg gaagacaact gggacggcta cacgggcttc attcacaacg tggtttacga
     5821 aaaccacctg aaaaaccacg aagcaccgga agactgcgaa ttttatatgt gcggcccgcc
     5881 gattatgaac cagtccgtca tcaaaatgct caaagacttg ggcgtggaag acgaaaacat
     5941 cctcttggac gatttcggcg gttaaatatt cggcttcaaa tacaaaagga agaaacctcg
     6001 gtttcttcct ttttctatcc gatgccgccc gaaccccctt cagacggcat cggcatagaa
     6061 acatatcggc cgttttgcga tataattcaa accatttgcc aaaaccgtca aacactatgc
     6121 cgtctgaaac acgcctgccg aactttatcc gcgtcttgat atttgccctg ggtttcatct
     6181 tcctgaacgc ctgttcggaa caaaccgcgc aaaccgttac cctgcaaggc gaaacgatgg
     6241 gcacgaccta taccgtcaaa tacctttcaa acaatcggga caaacttccc tcccctgccg
     6301 aaatacaaaa gcgcatcgat gacgcgctta aagaagtcaa ccggcagatg tccacctatc
     6361 agcccgactc cgaaatcagc cggttcaacc aaaccacagc cgacaaaccc ctccgcattt
     6421 caagcgactt cgcacacgtt accgccgaag ccatccgcct gaaccgcctg acgcacggcg
     6481 cactggacgt aaccgtcggc cctttggtca acctttgggg attcgggccc gacaaatccg
     6541 ttacccagga accgacaccg gaacaaatca aacaagcagc atcttatacg ggcatagaca
     6601 aaatcatttt gaaacaaggc aaagattacg cttccttgag caaaacccac cccaaagcct
     6661 atttggattt atcttcgatt gccaaaggct tcggcgttga taaagttgcg ggcgaactgg
     6721 aaaaatacgg cattcaaaat tatctggtcg aaatcggcgg cgagttgcac ggcaaaggca
     6781 aaaacgcgcg cggcgaaccg tggcgcatcg gtatcgagca acccaatatc atccaaggcg
     6841 gcaatacgca gattatcgtc ccgctgaaca accgttcgct tgccacttcc ggcgattacc
     6901 gtattttcca cgtcgataaa agcggcaaac gcctctccca tatcattaat ccgaacaaca
     6961 aacgacccat cagccacaac ctcgcctcca tcagcgtggt cgcagacagt gcgatgacgg
     7021 cggacggctt gtccacagga ttattcgtat tgggcgaaac cgaagcctta aagctggcag
     7081 agcgcgaaaa actcgctgtt ttcctgattg tcagggataa aggcggctac cgcaccgcca
     7141 tgtcttccga atttgaaaaa ctgctccgct aaacatacaa accgattagg aaacaccatg
     7201 aaaaccctgc tccttacctt cggcatcttc ctgaccgtca tcatcggtat ggcggtcggc
     7261 tatattttct ccaaacgcag catcaaaggc agttgcggcg gcattactgc cttgggtatg
     7321 aaaaaaatgt gcgactgcga cacaccttgc gacaccctgc aaaagaaact ggatgaagaa
     7381 aaacaggcag gcggcatacg ggttgaccgt taacgccgaa acaaaatgcc gtctgaagcc
     7441 cgacaggcgg gttcagacgg cattgccgct ttacttcgat cggtttatac gcaaaacgcg
     7501 cttcaggaaa taaatcagat tattccccag acgccttcag gcttccttct gtgccaccgt
     7561 aaccatagcc gggcgcaaca cgcggtcgga cagcgtataa cccttcttca tcacgcccac
     7621 cacggtattg ggttcctgtt cgcttgccac cgcctgcatc gcctgatgga tattcggatc
     7681 gagcttatcg cccgctttag gattgatttc cttgatttgc gtagcatcaa atgctttctg
     7741 caactcgttc aaagtcatct gcacgcccat tttcagcgca tcgaaattgc cgctctgatc
     7801 caaaagcgcc atttccaaat aatccttgac cggcagcatt tccacggcaa acttctgtcc
     7861 ggcgaacttg tgcgtatccg caatttcctg ctggtggcgg cggcgcaggt tttgctcgtt
     7921 tgccaaagcg cgcagctgct cgtctttcaa ctgcgcttcc agctcggcaa tccgcgcctg
     7981 caaatcctca taagccgact ctgcggcagc ctgttcctgc acaccgtccg catttcctac
     8041 tgtctcgacg gtttccaccg cctccacatt ttcaaccgct tcttcactgt tttgctgctg
     8101 tgtctgttcg ctcatatcgt atcccttaaa acaaattgga aatcaaaatc cagttattac
     8161 ccgcgcaaga taaggacatt ttcaagaaac ttcaagcaaa agcagagaat ttcaaattca
     8221 ttttcaaatc ccctaccgaa aaaataatat agacggtaag atactgccga ccatatcgat
     8281 tttcaaacgt catcactcat gaaaaaagac catttaaaca caaccggttt cgacctctgg
     8341 cacaccatcc gcgaagaaac cgcggccgcg gtctccgccg aaccgatgct ggcaagtttt
     8401 ttgcaccaaa ccgtgttgcg ccacgagtcc ctcggctccg tccttgccta ccacctttcc
     8461 agcaaactcg gcagcccgat tatggacgtg cgtgcgctgt ttgaaatcta ccagcaggcg
     8521 ttgggtagcg acacccaaat cggcaaatgc gtcgaagcag acttaaaagc catctacgaa
     8581 cgcgatcccg cctgcgacga atattcgctg ccgcttttat atttcaaagg cttccacgcg
     8641 attcaggcac accgcatcaa ccaccggctg tatctcgacg ggcgcaaaac gctggcgtat
     8701 ttcctgcaaa accgtatgtc cgaagtattc ggcgtggaca tccatcccgc cgcccgtttc
     8761 ggatacgggc tgatgctcga ccacgccacc ggctttgttg ccggcgaaac cgccgtgttg
     8821 ggcaacaata tttcgatttt gcacggcgta acgctcggcg gttcgggcaa agaaggcggc
     8881 gaccgccacc ctaaaatcgg cgacggcgtg atgatcggcg caaacgcctc gatattgggc
     8941 aatatccgca tcggcagcaa tgccaaaatc ggggcgggca gcgtcgtggt ttcagacgtg
     9001 ccgccgtcca tcacggttgt cggcgtaccc gccaaacccg tggcgcgatc gctcaaaaca
     9061 ccgtcggcgg atatggatca aaatatccag tttaccgaaa tcgactttat gatttgaata
     9121 tccgcaccaa tgccgtctga aacgccaaac gggcttcaga cgacattgcc cgcttcaagc
     9181 ctgcgccttg atatgccgcc gatgtcggca cttgccagtc ggacaaaatg ccttttattc
     9241 aatacacccg attcccacgc aaccggaaat atagtggatt aacaaaaatc aggacaaggc
     9301 gacgaagccg cagacagtac g
//
LOCUS       DAWFAN010000072         9299 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWFAN010000072 DAWFAN010000000
VERSION     DAWFAN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.072"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(91..1371)
                     /locus_tag="WGO65_001214"
     CDS             complement(91..1371)
                     /locus_tag="WGO65_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ0327508.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRSCVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1593..2213)
                     /locus_tag="WGO65_001215"
     CDS             complement(1593..2213)
                     /locus_tag="WGO65_001215"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ0327509.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIKKVLDGHFGC"
     gene            complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WGO65_001216"
     CDS             complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WGO65_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ0327510.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3352..3924
                     /gene="ampD"
                     /locus_tag="WGO65_001217"
     CDS             3352..3924
                     /gene="ampD"
                     /locus_tag="WGO65_001217"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ0327511.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            4118..5404
                     /locus_tag="WGO65_001218"
     CDS             4118..5404
                     /locus_tag="WGO65_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ0327512.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5552..8077
                     /gene="ligA"
                     /locus_tag="WGO65_001219"
     CDS             5552..8077
                     /gene="ligA"
                     /locus_tag="WGO65_001219"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ0327513.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGLITEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTVVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDIVVVRRAGDVIPEVVRVIFERRPMQETDVAASDGIGHRQDDLFAET
                     PSANQTQTVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQTPSED
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSILSEAELLTLLG"
     gene            8110..>9299
                     /locus_tag="WGO65_001220"
     CDS             8110..>9299
                     /locus_tag="WGO65_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ0327514.1"
                     /translation="MSTFPNHTMHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFPAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLARLGRVHN
                     GKEALTAIDTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGTLEGAGFIHYETSAFAQNNKQCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPAAMLQERTGVPSAKIMAQIETARQKGLLETNPAVFRPTEKGRLFLN
                     DLLQ"
BASE COUNT         2205 a         2815 c         2322 g         1957 t
ORIGIN      
        1 ttgtcctgat ttttgttaat ccactatatc aatctgacag caaaatgccg cctgaaaaca
       61 gttcagacgg cattttaaac cgcaaacggc ttacagcttc cattcgctca gcttggcagc
      121 gtaagcttcc aaatctgcaa tcggacgggt tgccacgccg ctttccatcg ctgctttggc
      181 ggcagccgta gcaacgcgcg gcagcaggcg ggaatcgaac ggagtcggaa tcaggtattc
      241 cgcgccgaat tcaaatttct taccgtaagc ggcaaccact tcttcggtta cttcttccat
      301 cgccaaatcc gccaaagcat acacgcagga gcgtttcatt tcttcgttga tggtcgtcgc
      361 gccgacatcc aacgcgccac ggaagatgaa tgggaagcac aatacgttgt tcacttggtt
      421 cgggaagtcg gaacggccgg taccgataac cacgtccgga cgggtttctt ttgccagcgg
      481 cggcaggatt tccggattcg ggttggccat ggcgaacacg atgggttttt cgttcatcgt
      541 gttcaacatt tcgggggtca gcaggttcgc gccggagagg cccaagaaga tgtctttacc
      601 tttgacggca tcggcaagta cgcgctggcc attgtcttca acggcgtaga attttttgga
      661 ttcgtccatg cgatctttgt cttcgcgggt ttggtaaatc acgcctttgg agtcgcaaac
      721 ggtcacgttt tcgcgtttca agcccaaatc cagcaattgg ttcaagcagg caatcgcggc
      781 cgcacctgcg ccggaacaca ccaaagtcgc ttcttcgatt ttacggccgg taaaacgcag
      841 ggcgttcaat acggcagcgg cggtaatgat ggccgtgccg tgctggtcgt cgtggaatac
      901 ggggattttg cagcgtttgc gtaattcgcg ttcgatgtag aaacactcgg gtgctttgat
      961 gtcttcgagg ttgatgccgc cgaaggtcgg ctctaaagcg gcgatgatgt ccacgagttt
     1021 ttgcgggtct ttttcatcga tttcgatgtc gaacacgtcc acaccggcga attttttgaa
     1081 caatacgcct ttgccttcca tcacgggttt gcccgccagc gcgccgatgt cgcccaagcc
     1141 caaaacggcc gtaccgttgg aaatgacggc gaccaagttg cctttggcgg tgtatttgta
     1201 ggcattttgc ggatcggcat ggatttccat acaaggagcg gctacgcccg gagagtacgc
     1261 caacgccaaa tctttgtcgg tcgccaaaga tttggtcggg gtaacggaaa ttttgcccgg
     1321 aacgggtaat tcgtggaact tgagggcggc ttctttcaat gagttttcca tgtgtcaatc
     1381 ctgttgcgag aaaaagattt gaatcggact gtccgaaacg ctttcgggtc atcatgttac
     1441 gggcggttcg ttaatattgc cgacgcatcc tgccttgtaa ccgagcgtaa ttctatcatg
     1501 ccgaaagttt ggcgcatacc gccattttca cctgcggcac tgccgtttga ttttcggcgc
     1561 gggtggatgc gcttgtttca atatttgccg cattaacagc cgaaatgtcc gtccaacact
     1621 ttttttatgt tgtttctgac ttcatccaag ctgcggttac tgtcgataat ggcgtaccgt
     1681 tcgggacagg cggcggcacg gttcagataa acgctgcgca cacgcatgaa gaaatctgcc
     1741 tgttcctgct cgaaccggtc tttctcgcgc gactgtccga tacgcgccat cgacacttcc
     1801 agcggcacat ccaacagcag ggtcaaatcg gggcgcaaac cgccctgcac ccaatgttcc
     1861 aaaatttcaa tgtcttcaga cggcatcccc cgcccgccgc cctgataggc gaaggttgcg
     1921 tcggtaaaac ggtcggacac gacatggatg ccgtctgaaa gtgcaggcaa tatcacgtct
     1981 tcaatatgct gcattctggc ggcgaacatc atcagtgttt ccgcacgcaa accggctttg
     2041 gtttcagggt tgagcaggat ttcgcgcaag gcctcaccga ccggcgttcc gcccggttcg
     2101 cgcgtgaaca gcacgggcag ccccctccgt tcaaaccatg ccttgatgac ggcaaggttg
     2161 gtggatttgc cggcaccgtc tatgccgtcc aaagtgatga attgcggttt catgcttgca
     2221 gttccggtaa gggtatgccg tccgaaaaca caaacttttc agacggcatg gtttattttt
     2281 tcaaaatata tttgcgaacg gcggcgttgt gttcggtcaa atcatggctg aactggctca
     2341 agcccgtacc gtccattttg gacacgaaat acaggtattt ttcaccggac ggatgggcgg
     2401 cggcatcgag tgccgccttg ccgggcagcg cgatcggggt tggcggcaga ccgccgcgcg
     2461 tgtaggtgtt gtacggcgtg tcgcggcgca ggtcggcttt acggattttg cccttgtatg
     2521 ccgcacccat gccgtaaatc acggacgggt cggtttgcag gcgcataccg attttcaggc
     2581 ggttgacgaa gacggaagcg acatggtcgc ggtcggcttc atgccctgtt tccttttcga
     2641 tcaggctcgc cataatcagc atttcataag ggtttttata aggcagcccg tcctgcctgc
     2701 tttcccatgc ctcattcagt cggcgttgca tcgccttgta ggcgatttgg taaatccgta
     2761 aatcgctgcc gcccgcatcg atttcgtagc tgtcggggaa aaactgccct tcaggattgc
     2821 cgctgaaggc atcaggggca acttccgcca tcagtttttc attgctccag cctttggtgt
     2881 cgtgttcgat gtcgggcgtt gcgtcgatga ctttcctcat atgcgaaaaa cgcgaacctt
     2941 cgataatctg cacggtaacg gaatccggcc tgccgccgcg cattttctgc aagatatccc
     3001 aagcagacac ttccgaaggc agtctgtacg tccccgtatg cagcctgttg tgcacaccca
     3061 aaacgtaggc cgccgccgtc aaaacatgcc tgctgaacac gatgcggtct tcggcaagtt
     3121 tcctgccgac cgacgaaata ccctggtttt tggcaatttt aatcctgtat gccctgccgt
     3181 tgtctttagg gacgaaaagc agcgcggcga aaacggctgc cgatacggtc aaaaaaacgg
     3241 cagaccattt caacaatttt ctcaacatgg taggattccc agcattcgga atacgtctta
     3301 aaattcgggc gacagtataa cccaaattgc ccgccggaca ggaagaacat catggacaat
     3361 cacgccgaag cacactggca aaacggctgg cttcaaagca tacgccatac cccgtcgccc
     3421 aatttcagcc cgagggaaac gggggaaacg gtttccctga tcgtgttgca caacatttcg
     3481 ctgccgccgt tcgaatatgg tacggatgcg gtggaaaagc tgtttgccaa ccggctcgac
     3541 cccgacggac atccgttctt cagcctgata cacactttgc gcgtatccag ccatttttta
     3601 atcaaacgcg acggcgaaac ggtacagttc gtttcctgcg acaatatggc ataccatgcc
     3661 ggcgtatcct cgtttcgcgg acgggaaaaa tgcaacgcat tttccatcgg catcgaactg
     3721 gaaggctgcg atttcgaacc ctttaccgaa gcgcaatacc gttcgctcga agccttgttg
     3781 gacgcaatct gccgccgcta ccccatcacg gcggtaacgg ggcaccaaga catcgcgccc
     3841 ggccgcaaaa ccgaccccgg tcattttttc gactggcggc ggatacgggg gaaagggttt
     3901 cccgtggaca gaaatgccgt ctgaaaacca cagattttag atggcatttc ggaaaatttc
     3961 cgcttatttc gacacactgc tgtcagaccc tgcaattttc gttataattc aaacgttata
     4021 accggccgtt ccgtcagact gccggaaacg caagcccgat ttaaccgcag tacccgcact
     4081 ttgcgcatcc tgcatcccga aatttaaagt gattgccatg atttacatcg tactgttcct
     4141 cgccgccgtc ctcgccgttg tcgcctacaa tatgtatcag gaaaaccaat accgcaaaaa
     4201 agtgcgcgac cagttcgggc actccgacaa agatgccctg ctcaacagca aaaccagcca
     4261 tgtccgcgac ggcaaaccgt ccggcgggcc agtcatgatg ccgaaacccc aaccggcggt
     4321 caaaaaaacg gcaaaacccc aagaccccgc catgcgcaac ctgcaagaac aggatgccgt
     4381 ctacatcgcc aagcagaaac aggcaaaagc ctccccgttc aaaaccgaaa tcgaaaccgc
     4441 cttggaagaa agcggcatta tcggcaactc cgcccacacc gtttccgaac cccaaaccgg
     4501 acattccgca ccgaaacctg ccgacgcgcc ggcaaaacct gcacccgttc cgcaaacacc
     4561 tgcaaaaccg ctgattacgc tcaaagaact gtcaaaagtc gaattaccct ggtttgacgt
     4621 gcgcttcgac ttcatctcct atatcgcgct gaccgaagcc aaagaactgc acgcactgcc
     4681 gcgcctttcc aaccgctgcc gctaccagat tgtcggctgc accatggacg accatttcca
     4741 gattgccgaa cccatcccgg gcatccgcta tcaggcattt atcgtgggta ttcaggcagt
     4801 cagccgcaac ggacttgcct cgcaggaaga actctccgca ttcaaccgcc aggtggacgc
     4861 attcgcacaa agcatgggcg gtcagacgct gcacaccgac cttgccgcct ttatcgaagt
     4921 ggcttccgca ctggacgcat tctgcgcgcg cgtcgaccag accatcgcca tccatttggt
     4981 ttccccgacc agcatcagcg gcgtagaact gcgttccgcc gtaacgggcg tgggtttcgt
     5041 tttggaagac gacggcgcgt tccactatac cgacacgtcg ggctcgacca tgttctccat
     5101 ctgctcgctc aacaacgagc cgtttaccaa tgcccttttg gacaaccagt cctacaaagg
     5161 cttcagtatg ctgctcgaca tcccgcactc tccggcaggc gaaaaaacct tcgacgattt
     5221 gtttatggat ttggcggtac gcctgtccgg ccagttgaac ctgaatctgg tcaacgacaa
     5281 aatggaagaa gtttcgaccc aatggctcaa agacgtgcgc acttatgtat tggctcgtca
     5341 gtccgagatg ctcaaagtcg gtatcgaacc gggcggcaaa accgcattgc gcctgttctc
     5401 ctaaaatcga atgcaatcaa agcggatatg tcgttacggc atatccgctt tttttcgggt
     5461 aaaattcagc tgttttcaac ctatcatttt tcagacgacc tttgcccgtt tcatcccgta
     5521 tcagacaaag gtcgtctgaa aaccttctct catgaatcca aactcaaaac ataacactaa
     5581 ctttaccaat cttcttaaac cgtctgatag tgatataaaa caatttgccg cacaacacat
     5641 ccgccacctc accgacctgc tcaaccgcta tgcctacgaa tactacaccc tcgacgcgcc
     5701 cagcgtaccc gatgccgaat acgacaaatt attccgcgag ctcgaagcgt tggaacgaaa
     5761 ccatcccgag ctcaaactgc ccgacagccc gacccagcgc gtcggcggcg agcctctggc
     5821 gggatttgcc gaagtacgcc acgaagtgcc gatgctgtcg ctgaccaacg ccttttcccc
     5881 gcaagatgaa aacggcgtgt tcgaccatgc agaaatgtac gctttcgacc aacgcgtgcg
     5941 cgacggtttg gacggcggca aacccgaata cgttatcgaa cctaaattcg acggcctcgc
     6001 catcagcctg ctctaccgcg acggcgtatt ggtacaggcg gcaacgcgcg gcgacggcac
     6061 aacgggcgaa gacgttaccc aaaacatcaa aaccgtatcc aacatcccct tgcggctgca
     6121 cggcgaaaat acgcccgaac tcatcgaagt acgcggcgaa gtactgatgc aaaaagccga
     6181 ttttgccgcc ctcaataaaa gacaagccga aaacgggcaa aaaccctttg ccaatccgcg
     6241 caacgccgcc gccggcagcc tacgccaact cgattcgcgc atcaccgcgc agcgcaaact
     6301 gcactttttc ccctactccg ttgcccgcca gcaaggcggt ttgattacgg aagaacacat
     6361 ccaagaactc gcctatttcc aagcactcgg cttcagtctg cccaatggca atttcggctg
     6421 tttcaaaaat atcgatgaag tattggcgtt ttacgaacaa atgcagcaaa aacgccccga
     6481 actgccctac gaaatcgacg gcatggtcgt caaagtcaat agcttggcac aacagcgcga
     6541 actcggcttc atctcccgcg cgccgcgctg ggcgattgcc cacaaattcc ccgccgaaga
     6601 agccctgacc gtcgtcgaag cgattgacgt gcaaatcggg cgcaccggcg ccgttacccc
     6661 cgtcgcccgc ctgcaacccg tattcgtcgg cggcgtaacc gtaaccaacg ccaccctgca
     6721 caatcaggat gaagtatcgc gtaaagacgt tcgcgtcgga gacatcgtcg tcgtgcgccg
     6781 cgccggagac gtgattcccg aagtcgtgcg cgtgattttt gaacgccgcc cgatgcagga
     6841 aaccgacgtt gccgcttcag acggcatcgg gcatcggcag gacgatttgt tcgccgaaac
     6901 gccgtccgcc aatcaaaccc aaaccgttcc gctccacaag ccctaccgcc tgccgaccca
     6961 ctgccccatc tgccgcagcg aaatcgaacg cgaagaaggc gaagccgtcg cccgatgcag
     7021 cggcggcatg ctttgtcagg cacaacgcgc gcaaggctta atccacttcg cctcgcgcaa
     7081 agcgatggac atcgacggct tgggtgaaaa acaaatcgaa cagcttgtcg cccaagacct
     7141 cgtccgccac ttcgccgacc tctaccgcct cgatattccg accctgcaaa aaatgaagga
     7201 aacggcggac aaaaccgttg ccgaatcgga tcaaacgccg tctgaagaca gttcggtcgg
     7261 cgcgtcgggc aaacataaaa aacaaccgac caagtgggcg gaaaacatcc tcgcaggcat
     7321 agaatccggc aaaacacccg aactcgcccg cttcctgttc gcgctcggca tccgccacgt
     7381 cggcgaacgc accgccaaaa cgctggcgca ggcattcggc acattggaac gcgtccgccg
     7441 cgcccccgaa cccgtcctcg cctgcctgcc cgacatcggt acggtagtgg cgcattccat
     7501 tgcccacttc ttcgcccaag ccgaacagca ggcgatgata gacgaactgc tcgccgcagg
     7561 cgttgccccg caaacccaag ccgttaccat cccgcccgcc cgccacgccg aaccgcaacg
     7621 ctggatcgcc cgcctgcccg acttcaaaat tagcgaaaac aaagcccaag ccttatggga
     7681 actcgctggt aaaaacatag aaggtctaca aaacgacaaa gccctccccg ccgactggca
     7741 agcatggcgc agcgaaccac aaaacgccgc cctactcgaa aacctgaaaa ccttcttcgc
     7801 gcaaatgccg tctgaagacg aggcggcgca aggttcagac ggcatcaata aagccgtagc
     7861 gggcaaaacc ttcgtgttaa ctggcacact gcccaccctc aaacgcgacc aagcccaatc
     7921 cctaatcgaa gccgcaggcg gcaaagtttc cggcagcgtt tccaaaaaaa ccgattacgt
     7981 cgtcgctgga gaagccgcgg gcagcaagct ggaaaaagcc aatgccttgg gtgtttccat
     8041 cctcagcgaa gccgaactgc ttaccctgct cggctgaacc tgtgccgtct gaaccgcctt
     8101 cagacggcat tgtccacttt tccgaaccac accatgcaca ccatttcctt tccaaaccgc
     8161 acccgactca ccgccctgcc gcccctgtcg ctttacatcc acatcccgtg gtgcatcaaa
     8221 aaatgcccgt attgcgactt taattcccac agcctgaaaa acggattgcc cgaagccgcc
     8281 tatatcgacg cgctgctgac cgacttgcag cttgaattgc ccaatatttg gggcagaccg
     8341 gtggaaacga tctttttcgg cggcggtacg cccagcctgt ttccggcgga atcgattgac
     8401 cgtttgttga gcggcgtgcg ttcgctgttg cgcttgcagc cggaagcgga aattacgttg
     8461 gaagccaatc cggggacgtt tgaaatcgag aagtttcaag gatttaaaga cgcaggtatt
     8521 acgcggcttt ctattggcgt gcagagtttc aacgacgata tgcttgcgcg gctggggcgc
     8581 gtccacaacg gcaaagaagc cttaaccgcc atcgacaccg ccttgaaatt atttgataaa
     8641 gtcaatatcg acttgatgta tgccctgccg aaccagaccg ttcagacggc attggacgac
     8701 gtacaaaccg ccatcgctac gggcgcgacc cacatcagcg cgtatcatct gacgatggag
     8761 ccgaacacgc cgttcggtca tacgccgccg aaaggtttgc cacaagacga agccgccctc
     8821 gacatcgaag acgcagtaca cggcacgctg gaaggcgcgg gttttatcca ctacgaaaca
     8881 tcagcctttg cacaaaacaa caaacaatgc cgccacaatt tgaactactg gcagttcggc
     8941 gattatttag gcataggcgc gggcgcgcac ggcaaaatct cctatcccga ccgcatcgag
     9001 cgcaccgtcc gccgccgcca ccccaacgac tacctcgcct taatgcaaag ccaaccgagt
     9061 gaagccgtcg aacgcaaaac cgttgccgcc gaagatttgc cgttcgaatt catgatgaac
     9121 gccctgcgcc tgaccgacgg cgtgcccgcc gcgatgttgc aggaacgcac gggcgtaccg
     9181 agtgccaaaa tcatggctca aatcgaaacg gcaaggcaaa aaggtttatt ggaaaccaac
     9241 cccgccgtgt tccgcccaac cgaaaaagga cgcttgtttt taaacgattt gctgcaatg
//
LOCUS       DAWFAN010000073         9192 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWFAN010000073 DAWFAN010000000
VERSION     DAWFAN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.073"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WGO65_001221"
     CDS             complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WGO65_001221"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ0327515.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WGO65_001222"
     CDS             complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WGO65_001222"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ0327516.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(3195..3896)
                     /locus_tag="WGO65_001223"
     CDS             complement(3195..3896)
                     /locus_tag="WGO65_001223"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ0327517.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            4082..5194
                     /locus_tag="WGO65_001224"
     CDS             4082..5194
                     /locus_tag="WGO65_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0327518.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            5228..6205
                     /locus_tag="WGO65_001225"
     CDS             5228..6205
                     /locus_tag="WGO65_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ0327519.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            6209..6559
                     /locus_tag="WGO65_001226"
     CDS             6209..6559
                     /locus_tag="WGO65_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ0327520.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            6564..7343
                     /locus_tag="WGO65_001227"
     CDS             6564..7343
                     /locus_tag="WGO65_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0327521.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRYTAEHIAKLRNQTLEQVAAYTTENFYRLFQKVPDMRTV"
     gene            7369..7584
                     /locus_tag="WGO65_001228"
     CDS             7369..7584
                     /locus_tag="WGO65_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ0327522.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            7685..7996
                     /gene="grxD"
                     /locus_tag="WGO65_001229"
     CDS             7685..7996
                     /gene="grxD"
                     /locus_tag="WGO65_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ0327523.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            8102..8728
                     /gene="upp"
                     /locus_tag="WGO65_001230"
     CDS             8102..8728
                     /gene="upp"
                     /locus_tag="WGO65_001230"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ0327524.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLATELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            8751..9071
                     /locus_tag="WGO65_001231"
     CDS             8751..9071
                     /locus_tag="WGO65_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ0327525.1"
                     /translation="MNFQDYLATFSSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
BASE COUNT         2237 a         2373 c         2389 g         2193 t
ORIGIN      
        1 gtaggaaaat ccgacaacgt tagacccgcc tgcaaaggta aggaatagca aatgccgtct
       61 gaaactattt tcagacggca ttcttggctt cctggcctaa cggattgccg taccggccct
      121 gccgaaatcg ccgaagttca tcaaaatgaa cattgccttg ccgacaacca gcttgtcatc
      181 cacaaatccc cagtagcgag aatcggcact gttgtcgcgg ttgtcgccca tagcgaaata
      241 gcgtccttcg ggaactttgc acacgaaacc gctgccgtcg tcggcatatt ggcagtgttc
      301 caaaccgctt tgctctatgg aatatccgtt ttcagacata atatcggagg tatatttgcc
      361 caatacgggc agggaaacgg caggctgtcc ttcttttttc agaatattga aggatttgcc
      421 gtccaaaccg ctgtggaaca tatccgtatt gtggatttcg gatgggtcgg tgtcgtcggg
      481 ataacggtat gtgccgttag gaatatcgga agtgggtttg ccatttaccg tcaaaatctt
      541 atcccgatat tcgaccacat cgcccggaat gccgacaata cgcttgatgt aggtcatctc
      601 cggctgcaga ggataattaa aaacaacgac atcgccccgt tcgattttgc ctgtggggat
      661 aaatatattg tttaaaacgg gtacgcgcaa gccgtaggaa aatttgccga ccaaaatgaa
      721 atcgcccttg atcaggcccg ggcgcatgga gctggatgga atttggaacg gttcggcaat
      781 aaacgaccgg atgagaaaca ataccaaaac ggtagggaag aaactgccga aataatcgcc
      841 gaagtggctg ctttccgaga tttcgggatg agtcttcagg cggtatttgt atacccccca
      901 agccgtaccg cacaatacaa cgaaaatcag gaaaacggcg gtaaagctca taaacaggga
      961 caaagcggca aacacgccga ccgctgtcag gatataggcg tattcgaggc cggaactcca
     1021 ttcaccgttt tcctgccgct tcttgtcgct tttgaaataa aggatgatgc cggcaagcag
     1081 cgcggcagcc gcgcccgaca ttagcattgt gttcattgtt gttccttaat gcttaaaaac
     1141 ccgcctgtcc gtgcaaccgt tttaaggcgg caaattgcaa aatttgtttg cgggcgcgtg
     1201 cccctgaaat tagggcggtt tgaggggtgt tcccgacgcg ccgccctgtg tgccagagtt
     1261 atttgtcgct cacctgcaaa atcgccaaga acgcgctttg cggaatttcc acattgccca
     1321 cttgtttcat acggcgttta cctgcctttt gtttttcaag cagttttttc ttacgcgtaa
     1381 tatcgccgcc gtaacatttc gctaagacgt ttttacgcag tgctttgacg ttttcgcggg
     1441 cgataatctg gctgccgatg gcggcttgga cggcaatgtc gaacatttgg cgcggaatca
     1501 gctcgcgcat tttcgatgcc agctcgcggc ctcggtgaac cgcgctttga cggtgcacaa
     1561 tcaggcttaa tgcatcgact ttttcgccgt tgaccataat atccagctta atcaaatcag
     1621 acggttggaa ctctttgaaa tggtagtcca acgaagcata accgcgcgaa gtggatttga
     1681 gtttgtcaaa gaagtccatg acgacttcgt tcatgggcaa atcgtaagtc agcatcactt
     1741 ggcggcccat gtactgcata ttgacctgaa cgccgcgctt ttggttacac aaagtcatga
     1801 cgttgccgac gtattcctgc ggcacaagga tggtcgcggt aataatcggc tcgagtatgg
     1861 tttcgatgct gccgatgtcg ggcagtttgg acgggttttc aacttcgatt ttctcaccgt
     1921 ttttcaacac gacttcataa accaccgtcg gcgcggtggt aatcaaatcc atatcgaact
     1981 cgcgctccaa gcgttcctgc acgatttcca agtgcaacag acccaagaag ccgcaacgga
     2041 agccgaagcc caatgcctgg gaaacttcag gctcgaattt caacgaagcg tcgttaagct
     2101 gcaatttttc caaagcgtcg cgcaaggctt cgtagtcgtg gctttccacg ggataaagtc
     2161 cggcgaatac ttggctttgt acttcttgga agccgggcag cggctcagtg gcagggttgg
     2221 caaccagtgt aaccgtatcg ccgactttcg cctgacccaa ttcttttacg ccggtaatca
     2281 aaaagcccac ttcgccggct ttgagttctt gtttttgaac tgattttggt gtgaatacgc
     2341 ccagctgctc gacctgcgtt tccgccttgg tgctcataaa gcgcactttg tctttcagtt
     2401 tgatggtgcc gtttttcact cgaatcagca tgaccacgcc gacatagttg tcaaaccacg
     2461 aatcgacgat aaccgcttgc agcggcgcgt tttcgtcgcc ggtcggcgca gggattttgg
     2521 caacaatttc ttccaaaaca tcttccacgc cgataccgct tttggcggaa cactgtactg
     2581 caccgacggc atcgatgccg atgatgtctt cgatttcctg ttccacacgc tcggggtcgg
     2641 cggcgggcag gtcgattttg tttaaaacag gcacaacctc cacgcccaaa tcaatcgcgg
     2701 tgtaacagtt cgctacggtt tgcgcttcca cgccctgcga cgcgtcaacg accagaagcg
     2761 cgccttcgca agccgacagc gaacgggaaa cttcgtaaga gaagtcgacg tgtcccggcg
     2821 tgtcaatcaa attaagctga tacacctgcc cgtcgcgcgc tttatagttg agcgcggcgg
     2881 tttgcgcttt gatggtaatg ccgcgctctt tttcgatgtc catggaatcg agtacctgcg
     2941 tactcatttc gcgcaaatcc aaaccgccgc aatattggat gaagcggtcg gcaagcgtcg
     3001 atttgccgtg gtcgatgtgg gcaatgatgg agaaatttcg gatatttttc attagagttg
     3061 ttttgaatgt cggacagtgg gtttgggaaa tgccgtctga acaaacggcg ttgcgtccga
     3121 atatcgggtg caacgtggaa atagcccgat attctaacgg aaaaccgctg ttttggcatg
     3181 atttgataaa ggttttataa agatttgacg atttctgcca ccatttttgc agaacttgcc
     3241 gccgccgttt tcaaaaattc gtcaaagctg atgtctgcct tttcgtccgc cgaatcggaa
     3301 accgcgcgga tgatgacgaa aggcgtttcc aactgatgac aggtttgggc aatcgccgcc
     3361 gcttccattt ccactgcttt gactttgggg aagtgcttgc ggatttccgc cacgccttcg
     3421 ctgctgtgga cgaagcggtc gccgctgaca atcaggcctt gttctaccgc cgcgccttca
     3481 aacacttccg ccgcctgttt tgctttgccg accaagaggc cgtctgaaac aaatacggcg
     3541 ggcagttgcg gcacttgtcc ccaaacatag ccgaatgcgg ttacatcgac atcgtggtgc
     3601 gcgatttctg tgccgaccac gacatcgccg actttcaaac ccttgcccaa gccgcccgcg
     3661 ctgccggtgt tgataacgca gtccggtgcg aattgatgga taagccaagc cgttgcaacc
     3721 gccgcgttga ccttgccgat gccgctcaat gcaagcacca tgcgttttcc cgctaattcg
     3781 ccttcatagg cggcaaactt gccgaaagag acggctttga cattttccat catctcgcgc
     3841 aaaagctcga tttcttgttc cattgcgccg ataacggcta ctgttttcaa agacatattg
     3901 ctgacctgtt gtgaatttcg gatagaatgc ctgattatac acgctaacac ggcaggattg
     3961 agtggaggtg gtttgtccgt gccgtctgaa acggtttcag acggcacggc gggtttttgg
     4021 tagaatggga aggtacagat tgtttgaaga ttaggggacg aggatgttta ccgatgaaaa
     4081 tatgaccgca aaggaagaac tgttcgcatg gctgcgccat atgaacaaaa acaaaggttc
     4141 cgacctgttt gtgacgaccc atttcccgcc cgcaatgaag ctggacggca aaatcacccg
     4201 catcacggac gaaccgctga cggcggaaaa atgtatggaa atcgcctttt cgattatgag
     4261 tgcgaagcag gcggaagaat tttcatcgac caacgagtgc aacttcgcca tcagcctgcc
     4321 ggacaccagc cgcttccgcg tcaatgcgat gatacagcgc ggcgcgacgg cgttggtatt
     4381 ccgtgcgatt accagcaaga ttcccaagtt tgaaagcctg aacctgccgc cggtcttgaa
     4441 ggatgtcgcg ctgaaaaaac gcgggctggt tatttttgtc ggcggcaccg gctcgggcaa
     4501 atcgacttcg cttgcctcgc ttatcgatta ccgcaatgaa aattcgttcg gacatatcat
     4561 caccatcgaa gacccgatcg agtttgtcca cgaacacaaa aactgcatca tcacccagcg
     4621 cgaggtcggc gtggatacgg aaaactggat ggcggcgttg aaaaacacgc tgcgtcaggc
     4681 gccggatgtc atccttatcg gcgaaatccg cgaccgcgaa acgatggact acgccattgc
     4741 ctttgccgaa acggggcatt tgtgtatggc gacgctgcac gccaacagca ccaatcaggc
     4801 actcgaccgc atcatcaact ttttccccga ggagcggcgc gaacaattgc tgacggattt
     4861 gtcgctcaac cttcaggcgt ttatttcgca acgcctcgtt ccgcgagacg gcggcaaggg
     4921 cagggtggcg gcagtcgagg tgctgctcaa ttcgcccctg atttcggagt tgattcacaa
     4981 cggcaacatc catgaaatca aagaagtgat gaaaaaatcc actaccctgg gtatgcagac
     5041 cttcgatcaa cacctttacc aattgtatga aaaaggcgat atttccctgc aagaagcatt
     5101 gaaaaatgcc gattccgcac acgatttgcg tttggcggta cagttgcgca gccgccgcgc
     5161 gcaaagttcc agccccgatt tggaactgct ctgatggcgg tatggatttc cggacggatg
     5221 gtttgaaatg atttatccgt ggcataatga gcaatggcgg cagattgcgg aacattggga
     5281 gcgtcgtccc aatgcatggc tgtttgccgg caaaaaagat acggggaaaa ctacatttgc
     5341 ccgctttgcg gcgaaggcac tgttgtgcga aacccctgca ccgggctgca aaccctgtgg
     5401 cgaatgtatg tcctgccatc tgtttggaca gggaagccat cccgattttt acgaaatcac
     5461 ccccttgtcg gacgaacccg aaaacggacg caaactgttg cagatcaaaa tcgatgccgt
     5521 cagggaaatc atcgataatg tgtacctgac ttcggtacgg ggcggtttgc gcgtgattct
     5581 gattcatcct gcggaaagta tgaatgtcca agccgccaac agtttgttga aagtgttgga
     5641 agagccgccg ccacaagtgg tctttttgct ggtcagccac gcggcggaca aggttttacc
     5701 gaccattaaa agccgctgcc ggaagatggt tttgcccgcg ccttcccatg aagaggcatt
     5761 ggcatatctg cgtgaaaggg gtgtggcgga acctgaggaa cgtctggctt tccattccgg
     5821 agcgccgctg tttgatgagg cggacggtgt ccgtgcgttg cggattaaac tgttggatat
     5881 tttggcagaa ccaaggttgt tgaagatttt ggattacgcc gcgcttttcg ataaggaaaa
     5941 acttccgctc gccgtatttg tcgggtggat gcagaagtgg ctggtcgatt tgggattgtg
     6001 cctgcaacac atgaaacccg tctattatcc cgcttatgaa gacaggctgc ttcagacggt
     6061 atccggtttc cgtccgcgca atgtatttgc ggcggaggat atgctcaaac agcttgcccc
     6121 ctacgggttt catactttaa atgtcaaaat gcagatcgag catctgctca tcaactattt
     6181 ggaattgaag aaagagaacg ggtgaattat gtcagacgga caaaatattc cggcaaaaat
     6241 gatgtcgttg cagctgaaag acatgaatct gctgtacagc tcctacatgc cgtttttgga
     6301 acacggcggt ctgtttgtgc agaccaacga cgtattttcc atcggggacg atattctgct
     6361 tgccgtagaa atcctcaact tccccaaact gttcctgccg accaaagtcg cctggatcaa
     6421 tcctgcgcgt acttcctcca aacccaaagg ggtagggctg gcattcacaa aacacgaaaa
     6481 ctgcctgaaa gtcaaagatc agatcgaagt cgaactgggc aacacaatcg gcggcagcag
     6541 acctacgttt accatgtaac gccatgcata tcatcgattc gcactgccac ctcaattttg
     6601 aaggtttgaa agaacgcctg cccgaagttt tgtccaacat ggaagcaaac ggcgtggggc
     6661 aggcgctcgc catcagcgtc agcagggaaa gcttctccga agtctttgcc atcgccgaag
     6721 cgcacgaaca catctattgc accataggcg tacatcccga cagcaaggaa gccgaagaat
     6781 tttctattgc ggaaatggtt gccgcagccg cccatccgaa agtggtcggc attggcgaga
     6841 cgggcttgga ttattactgg tgcaaaggcg atttgtcctg gcagcacaaa cgctttgcag
     6901 accacatcga agcagccaat caaaccggac tgcccgttat cgtccatacg cgtgatgcgg
     6961 cggcggacac cttgtctatc ctgaaagaat gccgggttaa ttcgggcgtt atccactgtt
     7021 tttccgaaga catcggtttt gcccgtgcag caatggattt ggggctttat atttctttct
     7081 cgggaatcgt tacctttaaa aacgcaccct tggttcagga ggcggcgaaa tacgttccgg
     7141 acgaccgcat tttagtggaa accgatgcgc cgttcctcgc ccccgtaccc aagcgcggca
     7201 ggcagaacga accggctttc gtgcgttata ctgccgaaca tatcgccaaa ttgcggaacc
     7261 aaaccttgga acaggttgcg gcatacacga cggaaaactt ttaccggctg tttcaaaaag
     7321 tacccgatat gcggaccgtc tgaccctgta ccgacgataa ggaaaaccat gaaggcaatt
     7381 catccgtatg catgtccgcg ctgctgccgg ctgcctgcca acacgtttcg gacaggcatg
     7441 gcaaattccg cttccaaatt ctgcattgcc aaaggcggca gacgggaagt aaaaaaagac
     7501 gaaagcggcg gcggatatgc cctgtgccat ttgccggaca gcaggattgt cgaggagtgg
     7561 gaatatttcc gttcacaaca ttgatactgc gcgatatacg gcaaatattg tgggaagttt
     7621 ccgcttttgc gtataatgcg ccctacctga caaattttgt caactttatc aaaaggaata
     7681 agtgatggct tccatccacg accaaattaa agaagtagta acgacacacc gcgtcgtatt
     7741 gtttatgaaa ggtacgaagc agtttccgca atgcggtttc tcttcccgcg ccgtgcaaat
     7801 cctgaacgcg gcgggctgca ccgattacgt taccgtcaac gtattggaaa atcccgaagt
     7861 acgccaaggc attaaggaat acagcgactg gccgaccatc ccccaacttt atgtgaacgg
     7921 cgagtttgtc ggcggttcgg acatcctgat ggaaatgtat gaggcaggcg agctgcaaga
     7981 gctgctgaaa gcctgatgga ttcggcaatg ccgtctgaac gtgtttcaga cggcattttc
     8041 ttttccggca aatcaaaaaa aagtataatg gcgcgtctca aaatcacact ggaacaccgc
     8101 gatgaacgtt aatgttatca accatccgct cgtccgccac aaattaaccc tgatgaggga
     8161 ggcggattgc agcacctaca aattccggac gcttgccacc gagctggcgc gcctgatggc
     8221 atacgaggca agccgtgatt ttgaaatcga aaaatacctt atcgacggat ggtgcggtca
     8281 gattgaaggc gatcgcatca agggcaaaac attgaccgtc gttcccatcc tgcgtgcagg
     8341 tttgggtatg cttgacggcg tactcgacct gattccgact gccaaaatca gcgtagtcgg
     8401 actgcagcgc gacgaggaaa cgctgaaacc cgtttcctac ttcgagaaat tcgtggacag
     8461 catggacgaa cgtcctgccc tgattatcga cccgatgctg gcgacgggcg gttcgatggt
     8521 tgccaccatc gaccttttga aagccaaggg ctgcaaaaac atcaaggcac tggtgctggt
     8581 tgccgcgccc gagggtgtga aggcggtcaa cgacgcgcat cccgacgtta cgatttacac
     8641 cgccgcgctc gacagccact tgaacgagaa cggctacatc atccccggct tgggcgatgc
     8701 gggcgacaag attttcggca cacgctaact gactgatttt cggatttgat atgaatttcc
     8761 aagactatct cgccacattt tcttcaatcg accatctggg cggtttggac gttcaggatg
     8821 ccgacggcaa aacggttcac cacattcccg ccgttcaggg taagctcggt tcgctcaagc
     8881 tgtacaatgc tttggcagaa cgttttgacg gaaaattgga caaagaagcg gcagaacagg
     8941 gtttgatatg gtttgccgaa cacgttgccg acgcgcgcgc ccatcccggc aagcatccca
     9001 atatcgacct gcttgaaaaa gttgtgcaaa gcggcgaaac cttgctgctc aagccgcttg
     9061 ccgcgcaata attttcgacc atgccgtctg aaatccgttt cagacggcat tttgtcggaa
     9121 agaagaccgt aaaacgggca ttttcttttc tatttcagga tacgggcaat gatgtttcaa
     9181 cacacaggac ga
//
LOCUS       DAWFAN010000074         9077 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWFAN010000074 DAWFAN010000000
VERSION     DAWFAN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.074"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            214..993
                     /gene="pgeF"
                     /locus_tag="WGO65_001232"
     CDS             214..993
                     /gene="pgeF"
                     /locus_tag="WGO65_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ0327526.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1045..1524
                     /locus_tag="WGO65_001233"
     CDS             1045..1524
                     /locus_tag="WGO65_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327527.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1526..2524
                     /locus_tag="WGO65_001234"
     CDS             1526..2524
                     /locus_tag="WGO65_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ0327528.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSVQSVRNSLR
                     LWGEKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2645..3064
                     /locus_tag="WGO65_001235"
     CDS             2645..3064
                     /locus_tag="WGO65_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327529.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3140..3655
                     /locus_tag="WGO65_001236"
     CDS             3140..3655
                     /locus_tag="WGO65_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0327530.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAVGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3739..4623)
                     /locus_tag="WGO65_001237"
     CDS             complement(3739..4623)
                     /locus_tag="WGO65_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ0327531.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLESGIREKISSHLARGKVECKIQIQDTENGNQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTDALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIGALSEIFPALVEAHKEKIRARLAEAVGSIDNDRLQQEFALFVQKS
                     DVDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            4902..5048
                     /locus_tag="WGO65_001238"
     CDS             4902..5048
                     /locus_tag="WGO65_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327532.1"
                     /translation="MLIHYINSANLSEKQLMRQCLVHVCFLISAIAMVWTDKIVYGTT
                     HKPH"
     gene            complement(5107..5970)
                     /gene="rpoH"
                     /locus_tag="WGO65_001239"
     CDS             complement(5107..5970)
                     /gene="rpoH"
                     /locus_tag="WGO65_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ0327533.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(6234..7772)
                     /gene="lnt"
                     /locus_tag="WGO65_001240"
     CDS             complement(6234..7772)
                     /gene="lnt"
                     /locus_tag="WGO65_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ0327534.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(7841..8209)
                     /locus_tag="WGO65_001241"
     CDS             complement(7841..8209)
                     /locus_tag="WGO65_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ0327535.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            complement(8109..8567)
                     /locus_tag="WGO65_001242"
                     /pseudo
     CDS             complement(8109..8567)
                     /locus_tag="WGO65_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            8763..8999
                     /locus_tag="WGO65_001243"
                     /pseudo
     CDS             8763..8999
                     /locus_tag="WGO65_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
BASE COUNT         1992 a         2261 c         2674 g         2150 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactatat aaagctcgga gaaggtatat aaggacatca
       61 ttcctgcacc ggcaagaatc cgaacccgaa cgtttgaaaa caatcccgaa tctccgaatt
      121 cccgcctgtg cgggaatgac gaaaaaacaa gcatccattt gccccgaagg cagttaatca
      181 accctttccg ccacacacct attccaatat ccaatgaaaa ccatcacaga aaccctaaat
      241 ctcgccccga aaggcaaaaa cttcctgacc gccgattggc ccgcgcccgc caatgtgaaa
      301 accctgatta ccacgcgcaa cggcggcgtg agccaaggtg cgtatcagag tttgaacctc
      361 ggtacgcacg tcggcgacaa tcccgaagcc gtgcgccgca accgcgaaat cgtgcaacag
      421 caggtcggat tgcccgttgc ctacctcaat caaatccaca gtaccgtcgt cgtcaatgct
      481 gccgaagctt tgggaggcac acccgatgcg gacgcttccg tagacgacac gggcaaagtt
      541 gcctgtgctg tcatgactgc cgactgtctg cccgttctat tttgcgacag ggcgggtacg
      601 gcggttgccg ccgcacacgc gggctggcgc ggtttggcgg gcggcgtatt gcaaaacacc
      661 attgccgcga tgaaggttcc acccgtcgaa atgatggcgt atctcggtcc cgccatcagt
      721 gcggatgcgt ttgaagtcgg acaggatgtg tttgatgcgt tctgcacgcc catgcccgaa
      781 gccgccgccg catttgaagg cataggcggc ggcaaattcc ttgccgacct ttacgcgctc
      841 gcccgcctga ttctgaagcg cgaaggcgtg ggcggcgtat atggcggcac gcattgtacg
      901 gttttggaac gggatacttt cttttcctac cgccgcgacg gagcgacagg gcgtatggcg
      961 agcctgattt ggctggacgg caatgccgtc tgaacacgcc gctgatataa tctaccgact
     1021 ttgtgctttt gagaaaggca agccatgaac aaactgtttc ttactgccgc agtgctgatg
     1081 ctgggcgcgt gcggtttcca cctgaaaggt gcagacggca tttctccgcc gctgacctac
     1141 cggagctggc acatcgaagg cggacaggca ttgcagtttc ctttggaaac cgcgctgtat
     1201 caggcttcgg gcagggtgga cgatgctgcc ggcgcgcaga tgaccctgcg tatagacagc
     1261 gtttcccaaa acaaggaaac ctacaccgtt acccgtgcgg cagttatcaa cgaatatctt
     1321 ttaatcttga cggttgaagc gcaggtattg aaacgcggcg agccggtcgg caaaccgatg
     1381 accgtgtccg tccgccgcgt ccttgcttat gccgacaacg agatcttggg caaacaggaa
     1441 gaggaagcgg cattgtgggc ggaaatgcgg caggatgccg ccgaacagat tgtccgccgc
     1501 ctgacctttc tgaaggcgga atgacgtggc ggcacatatc ggacacattg atacggacgc
     1561 gcctttgaaa cccctgtacg tcatccacgg cgaggaagaa ctgttgcgta tcgaggcatt
     1621 ggacgcattg agggcggcgg cgaagaaaca aggttacctt aatcgggaag tttatacggc
     1681 agacaatgcc ttcgattgga acgaactgtt gcaaaccgca ggcagtgcgg gtctgtttgc
     1741 cgatttgaag ctgttggaac tgcatatccc caacggaaaa cccggcaaaa ccggcggcga
     1801 ggcgttgcag gattttgccg cccgcctgcc ggaagatacg gtaacgctgg ttttgctgcc
     1861 caaactggag aaaacccagc tccagtccaa atggtttgcc gcattggcgg caaaggggga
     1921 agtgtgggaa gccaaaccgg tcggcgcggc ggctttgccc caatggatac gcggacggct
     1981 ggacaaaatc ggtttgggta tcggggcaga cgcattggca ctgtttgccg agcgcgtgga
     2041 aggcaatctg ttggcggcgc gtcaggaaat cgacaagctc gggctgctgt atccgaaagg
     2101 gcataccgtc aatatcgatg aggcgcaaac cgccgttgcc aacgtcgccc gcttcgacgc
     2161 gttccaactg gcaggcgcgt ggatgaaggg cgatgtcctg cgcgtatgca ggcttttgga
     2221 cggattgcgg gaagagggcg aagaaccggt gctgttgctg tgggcggttg ccgaagacgt
     2281 gcggacgctg atccggcttg ctgccgccct gaagcagggg cagagtgtcc aatccgtacg
     2341 caacagtctc agactttggg gtgaaaagca gacgctcgca ccgcttgcag tcaagcggat
     2401 ttccgtcgtc cgcctgcttg acgcgctcaa aacctgcgcc caaatcgacc gaatcatcaa
     2461 aggtgcggaa gacggcgacg catggacggt attcaaacgg cttgtcgtgt cgctggcgga
     2521 ataaagcggt aatccccaaa atccgaaaat actgtaaaat accgttaatc ctgaaaagta
     2581 ttcaccaatc cgtccgaaaa catttcagac ggcacgacca cctcaataaa ggaacattaa
     2641 ccctatggac aataagacca aactgcgctt gggcggcctg attttactga ccaccgccgt
     2701 tttaagcctc attatcgtat tgattgtcga ctcctggccg cttgccatcc tgcttgccgc
     2761 cgtcatcgtc gccgccgctg cgggcggttt tgtttggaca tcccgccgac agcaacgcca
     2821 gtttatcgaa cgcctgaaaa aattcgacat cgatcccgaa aaaggcagaa tcaacgaggc
     2881 aaacctgcgc cgtatgtacc acagcggcgg acaacaccag aaagatgcga ttaccctgat
     2941 ctgcctgtcg caaaaatgtt cggtggacga ggcgcacgct atgttcaaaa aacgcccgac
     3001 acgtcaggaa atcaatcaaa tggcggcaaa acagtcgcgc ggtcagaaac gtccgcaccg
     3061 ttaaccgccg caaggcatct ttgcataaat gccgtctgaa gcctgttggc gtttcagacg
     3121 gcatattctg attgaaaaga tgatgacact gaaaaccgcc ccgctcaaac gccgctttgc
     3181 cgccatgctg tacgaaatgc tgctggtcgg tgcggcaacc tgtttggcag cattgtttgc
     3241 cggtattgcc gccatttttc tgaatcccgt ttctatcgcg gtttctgcat tggtaacgag
     3301 tatcctgata atgggatcat ggtggcttta ttttcgcgcc aactggcacg gtcaggggca
     3361 gaccttggcg atgaggacat ggaaaatcgg cttgtgcgac cttaacggca tacagccgtc
     3421 tttgcacctg ctgcgcctgc gctttatttg ggcgtgcata ttcatcgtct tcattcctat
     3481 gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg cggtcggtgc
     3541 ggcattgatt tggctgattt taccgtgggg gttcgcactg ctgaatcccg atcggcagtt
     3601 tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc cttaagcctt
     3661 tataccgcaa aggtttcaac ctgaaaaaat gccgtctgaa agggctttca gacggcattt
     3721 gcttatcggg gaaaccgatt attcgatatt ctgcacctgt tcgcgcatct gttcgatcag
     3781 gactttcagc tcgaccgagg cttgggtgca ttcggcggca atggatttgc tgcccaaagt
     3841 gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgttttccgc tgctgccttt
     3901 gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgcaggcggc tgaattcttc
     3961 atcgacatcg gatttttgga caaacagggc aaactcttgt tgcaggcggt cgttgtcgat
     4021 gctgccgacg gcttcggcaa ggcgggcgcg gattttttct ttatgcgctt ccactagcgc
     4081 ggggaagatt tcgctcaatg ccccgatgat ttcttccata gactcgaggc gttgcagcag
     4141 gtgttcgccc aattttttcc cttcgcgtcg gcgtgcggcg gtaaagtctt tcagtgccga
     4201 atcggtcaaa tcggcaataa cctttgccaa tgcgtccgta ttttcccttt ggcttgccaa
     4261 tacgccgggg aaacgcagga tgtcggcaac gcccagtttt gccaaatcgt gatgcttgcg
     4321 gaggtctttg ttgatttcgg caagctgtcc gaccagatcg cgattcagtt ccaaagactg
     4381 attgccgttt tccgtatctt gaatttggat tttgcattcg actttgccgc gtgccagatg
     4441 ggatgaaatt ttctcgcgga taccgctttc caaatggcgc aaatcatccg gcatcctgat
     4501 ttgaatgtcc aaaaagcggt ggttgacggc gcggatgtcg aggttgatgc gtttgctgcc
     4561 gcattccgcc gccgcgttgg caaagccggt catgctgtgg atgtggatat ttccgctgct
     4621 catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt atggcggcaa
     4681 gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc acgcgctttt
     4741 gttgctgcaa ggaagggaaa tgtatagtgg attaaccaaa accagtacgg cgttgcctcg
     4801 ccttagctca aagagaacga ttctctaagg tgttgaagca ccaagtgaat cggttccgta
     4861 cgatttgtac tgtctgcggc ttcgccgcct tgtcctgatt tttgttaatc cactatatca
     4921 attccgccaa tctgtcggaa aagcagctga tgcggcagtg tctggtgcat gtctgctttt
     4981 tgatttcggc gattgcgatg gtttggacgg ataaaatcgt gtacggcacg acgcacaaac
     5041 cgcattgatg tttaccaaat aaaatacccg acaaaacaat ttgtcgggta ttttattgcg
     5101 tatatttcaa accgcttcgg cttcttcggt caggaaacca cgcagtttct gcatggcttt
     5161 tgcttcgatt tggcggatgc gttcggcaga tacgccgtat tcggcggcaa gctggtgcag
     5221 cgtcagcccg ccgtcgtctt gaagccagcg gctttccaca atacggcggc tcctgtcatc
     5281 cagttgcgcc aaagcgtttt gtaaaccttc tgtttgcagg gcgtaatgcg cctgtttcga
     5341 tagttgtcgg ctcggttcgg aatcgtggtc ggcaagccag tcgatggggg cgaaactgtc
     5401 ctcgtcgtcg ctgttgtctg ccatgatggc gatgtcgtgt cccgtcatgc gctgttccat
     5461 ttccagaact tcggaaagtt tgacacccaa atcgtcggcg atgtcttgtg cctctttggg
     5521 agacagggcg ttgaggtttt tacgcatact gcgtaaattg aaaaacagct tgcgttgcgg
     5581 tttggtggtg gcaacgcgga caaggcgcca gttcctcagg atgaattcgt gaatttccgc
     5641 tttaatccaa tgcacggcaa atgaaaacag gcgtgcacct ctaccgggct cgtagcgttt
     5701 gaccgccttc atcagtccga tattgccttc ctgaatcagg tctgcctgat tcagcccgta
     5761 gccgtcatag ccgcgcgcga tggaaacgac gacgcgcagg tgggacagga tgagttgttt
     5821 ggcggcgttg aggtcgccct ttatccggcg ttcggcgagg cgggtttctt cttcctggga
     5881 cagcatcggg atgctgttga cggtgtggat gtattgttcg aggctgccgt tgccgctttg
     5941 gatggcgggt aatgcgaaag cgttattcat ttggggcatt tcctttcggc tgaaactgcg
     6001 tatcggcggt ttgctgtgtt gggatgcagt atatcactgc ttggcttgta ttttgtatgt
     6061 ttggcaggag atatgcgcta aggtttgaaa gacaggaaaa ttttgtaagg caggtttgat
     6121 tgattttgta aacctgatgg ctcaatccga tttcggaatt atattacata cgtagttgta
     6181 tgtaaacagc cggtttgaaa aaagacagcc cgtccggacg ggctgtgcag gtatcagtgt
     6241 tctttgtttc ggaagatgaa aagaatcagt gcggctaggg tcaatatgcc catcaaccac
     6301 catgaactgc cggttttcat atagggtgtt tcgccgacat agcctttgat gtgtccttcc
     6361 aatacggttt ccgtatcggg ttgggcttgg gcgatgatgt tgcctttggg ggagatgatg
     6421 gcggttgcgc cggtgttggt ggcacggacc atatagcgtc cgagttccat agcccgtgcc
     6481 tgcgattgtt ggaggtgctg gtacatggcg ttggattttc cgtaccacgc catattgctg
     6541 gcattggcaa gcagggtggc atctttggcg gcggcaatca gttcgtcgcc gaatccgtct
     6601 tcgtaacaga tgttgaaggc gattttttgg tttttcatca gcagggcgga ttgcttaccg
     6661 ccgcctttgc ggaagtcgga aaggggcata ttcatcattt tgtaaagcgg cgtggtcagg
     6721 aagggcagcg gtttgtattc gccgaagggg acgaggtggt ttttggcgta gtaggggatg
     6781 ccgtcctgat tgttttcctg ataaccggtc aggttgatga cggcgttttc gtaaccgttg
     6841 ccgtccgaag tgtattggct gatgccgacg gcgagcgcgc tgccgttgtt ttgcgcctgt
     6901 tcggcaaatt ttgccagtat gttttccggc aggttttggc gcataacggg gatggcggtt
     6961 tcgggcagga tgacgatgtc ggcagtggtt ttgccgacct gttcgtaata tttctgtatg
     7021 gtcgggatga cttggtcttc acgccatttg agggtttggt cgatgttgcc ttgaagcagg
     7081 gcgacggtgc tgcggctgcc gtcggggcgg gtgaagtcgg tttgtcgggc ggtgtagcct
     7141 gcggcaagca gggcggcaat caggataatt ggaagcaggc gtttgcccga acgtgcggtg
     7201 ttatcactcg ccaaaaccag ccagacaccg agaaaggcgg ttgccagtgt aaccatgtgg
     7261 atgccgccca atggggcaaa gccggcgagc gggctgtccg gggtgatttg ggagtagccg
     7321 attgcgcccc agccgaatcc ggtcaggaaa cgttcgcggg caaactcggt cagtgtccac
     7381 aggatgggca gtaccaaacc gatttttatg ccccgaggca gggtaaattt tttccacagc
     7441 cagaaacaca gtgccggata aagggcaagg taggcgggga gtaggaaggt cagcggtacg
     7501 gcatagaggt cgggcaggcc ggaaacgtcg tgcagggcgg tgtgtatcca gtagaactgt
     7561 gtcgtgtatg cggtcaggcc gaacaggtag gcggaagaga cagcaaaacg cggacgcagt
     7621 tcgatgaggc ggacgaaggc accgaaaatc aagggcatca gccaaaagtg gtagtagggt
     7681 gcgaaggtaa agggggtggc ggcggcaaaa aggatgagca aaggccagta gagggcgggg
     7741 tgctgccagt atttgtccag tttggaaacc atattcatct gtctgttcgg aagataccgt
     7801 ctgaacatct ttcaaacggc atcggtattt gaaaaagaaa tcaatgcctg ccgaaacgat
     7861 tcatcagcgg caaggcgggg gcgcaggcaa tcgaaccgga aagcagcccg acaacgaggt
     7921 tggcgaagta ctccgcccag ccagcgtccc agtgttgcgc gtgcaggaaa tcgtgcagaa
     7981 ggccgaggtt gtgggcgacc agtacgccgc cgataagaaa catggcaagc gtgccgacca
     8041 cgctcaaacc gcgcataaag caaggcataa aggcagtcag catttgcccc aaactgcgcg
     8101 aaaaggtttg tgggcggcgc atcagcagca tgcctaagtc gtcgagtttg acgatgacgg
     8161 caacgattcc gtacaccaaa acagtcatgc cgatgccgat tgccgccatt acgagcatgc
     8221 gcgtcatgtc ggtgcagaaa cttgtgcagc agcttttcta cgccttcaaa gcacagataa
     8281 atgccgcctg ccgtcaaaag cggcgtaatg agttgcggca ggaaggcgga aagcagcagg
     8341 gccgcaggca ccaaaaccgg cttgttggaa aaagaacctt tcgccatcga ccaaacaatc
     8401 ggcaactcgc gttctgccga tacgcccgta acacggttgg cattgggtgc caaatcgtcg
     8461 ccgaccacgc cggcggtttt ctttgcggcg gctttggtca tcagggcaac atcgtccaaa
     8521 acggcggtga tgtcgtccgg cagggtaaat agtgaggcaa atgccattaa agaatcctga
     8581 aatgcggcgc aaagtccgac attatatagg agaacgcgga tttgggcggt ttcaggcggc
     8641 atgaaacagg aaaatgccgt ctgaacgctg tggcggacgt gaagtaaagt ttcgtgaaaa
     8701 gaaaatgccg tgttacagtc tttcgatttt aatttaatga attttaaggg agaatcgtta
     8761 gcgtggattg gatgggcagt ctgttcctgc cgggtggcgc actgttgttt ctgagcgtgg
     8821 tttcgaccac tttacccgca cgtttgggaa tgcctttgct gctggtttct cccgccggcg
     8881 tgttggacgg ggcggcggaa gccttggcga ttgcggcgtt cctgatgctg gtcgcgcgtc
     8941 cgccggcagt gttcggcggt ttgtggaaat tcaattacag cctgcgtgaa aaggcgtata
     9001 gccgaatcga aatgcagtcc gacaccgtgc ttcaggcggg gatttggcgt ggtacatcct
     9061 gcccgacggc aaggtcg
//
LOCUS       DAWFAN010000075         9058 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWFAN010000075 DAWFAN010000000
VERSION     DAWFAN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.075"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(120..1337)
                     /locus_tag="WGO65_001244"
     CDS             complement(120..1337)
                     /locus_tag="WGO65_001244"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ0327536.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVEAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEAHRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1539..2267)
                     /gene="rph"
                     /locus_tag="WGO65_001245"
     CDS             complement(1539..2267)
                     /gene="rph"
                     /locus_tag="WGO65_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ0327537.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2349..2813)
                     /locus_tag="WGO65_001246"
     CDS             complement(2349..2813)
                     /locus_tag="WGO65_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ0327538.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNSARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3030..3806
                     /locus_tag="WGO65_001247"
     CDS             3030..3806
                     /locus_tag="WGO65_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ0327539.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMATYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKAKMRVPCVFSTENITPPQSGVAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            3944..4471
                     /locus_tag="WGO65_001248"
     CDS             3944..4471
                     /locus_tag="WGO65_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327540.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKIYILRISFT
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRAS
                     TDYIVSRNLKFRHLY"
     gene            4642..5496
                     /locus_tag="WGO65_001249"
     CDS             4642..5496
                     /locus_tag="WGO65_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ0327541.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFISLLELAKEGLVRIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5537..6745
                     /gene="pncB"
                     /locus_tag="WGO65_001250"
     CDS             5537..6745
                     /gene="pncB"
                     /locus_tag="WGO65_001250"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ0327542.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6837..8555
                     /gene="argS"
                     /locus_tag="WGO65_001251"
     CDS             6837..8555
                     /gene="argS"
                     /locus_tag="WGO65_001251"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ0327543.1"
                     /translation="MNLHQTVEREAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVAEALADNAVIESAEVAGPGFINLRLRPDFLAQNIQTALNDT
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEAPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDADAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
BASE COUNT         2185 a         2503 c         2249 g         2121 t
ORIGIN      
        1 tttaatccac tatatttttt gttccaaagt caaaatatgc cgtccgaaca ttcgggcggc
       61 agacaaaacg gcactgcccg ataaaggcag tgccgttgtc cgtttcaaac cgtgaaacat
      121 cagcccaaat taaaggcttt atgcaatacc ctggttgcca gttccatgta tttttcatca
      181 atcaatacgg aaactttgat ttcggaggtg gaaatcattt ggatgttgat accctcttcg
      241 gcgagcgtgc ggaagatttt ggcggctaca ccgacgtgcg aacgcatacc caaaccgact
      301 gcggagactt tgcatacggt gtcgtcgcca tcaatagaag ccgcgccgat actgtcttgg
      361 cgttccgaca ggatttccaa agtctgcttg taatcgccgc gcggtacggt aaaggaaaaa
      421 tcggttgtgc cttcgctgcc gacattttgg ataatcatat cgacttcaat gttggcatcg
      481 gcaaccgcgc ctaaaatctg ataggcaacg ccgggtttgt cgggtacgcc gcgcacgttg
      541 atgcgggctt ggtttttatc gaatgcgata ccggttacgg cagctctttc catgttgtcg
      601 tcctcttcaa aggtaattaa ggtgccattg ccgccgtctt gcaggctgct cagtacgcgc
      661 aggcgcactt tgtattttcc ggcgaattct actgaacgga tttgcaaaac tttcgaaccg
      721 aggcttgcca gttcgatcat ttcttcaaat gtaaccgtat ccatgcggtg cgcttcgggt
      781 acgacgcggg ggtcggttgt gtaaacgccg tccacgtcgg tatagatttg gcattcgtcc
      841 gctttgaggg ctgcggcaag cgcaacggcg gaagtgtcgg aaccgccgcg tccgagcgtg
      901 gaaatatcgc cttcactgct gatgccttgg aagccggcaa cgatgacgac tttgccggcg
      961 gtaaggtcgg cacgcatttt ttcgtcatca atgctttcga tgcgggcttt ggtgtgggcg
     1021 gtatcggttt tgagggcgac ctgccagccg gtgtagcttt tggcttccac gccgatgtct
     1081 ttcaatgcca tcgccaaaag accgatggtg acttgttcgc cggtggacaa aaccacgtcc
     1141 aattcgcgcg gatcgggatg ctcctgcatt tcgtgcgcca acgcgaccag gcggttggtt
     1201 tcaccgctca tggcggatac gacgacgacg atgtcgtgtc cttcggcgcg ggctttggcg
     1261 acacgtttgg ccacgttttt aatgcgttcg ggcgagccta cggatgtgcc gccgtatttg
     1321 tgtacgatta acgccatgtt tcgtgctttc ttgtgtggga ttggtcgggc agcatggttt
     1381 gctggaaaag cggctattat tactattttt tacatgaagt tcaagaacgg actgcgcttt
     1441 cccgcctgcc gtttgacagc ggtcagcgaa aaacctgttc tttcagattg ttgacaaaat
     1501 gccgtctgaa cggttttcag acggcatccg gacgacaatc aggcggcgga caacgcattt
     1561 tgctggtgtt gcagcagttc gcctatgcct ttttgcgcca gtgcaaccag tttgcccaat
     1621 tcgtccaaac tgaacggcgc gtcttccgcc gtaccttgga tttcgatgat ttttcccgat
     1681 gcggtcatga cgatattcac atcactgtcg caaccggagt cttcgggata atccaaatcc
     1741 aaaagcggca cgccgttcac tacgcctact gacacagcgg caacggcttc gcggatgggg
     1801 ttttcactca aaatgccgtc tgaaaccagt ttgccgacgg cgatttgcag cgcgacaaac
     1861 gcaccggtaa tcgaagccgt gcgcgtaccg ccatctgcct gaatcacatc gcagtcaatc
     1921 aagatttgtc gttcgccgag tttttccata tccacgaccg cgcgtagcga acgcccgata
     1981 aggcgttgga tttcttgtgt gcgcccggac tgtttgcccg ccgaagcttc gcggcgcatc
     2041 cgggaagcag ttgaggcagg cagcatcccg tattccgccg ttacccagcc ttggttttta
     2101 ccgcgcagaa acggcgggac gttttcatct atggaagcgg tacaaatcac tttggtattg
     2161 ccgcattcaa taaggcacga accgtccgta tgcggcagga aatgaggggt gattttgata
     2221 tcgcgcaggc tgtcggcggc gcgcgagatg cggatgtaat caggcatact gccctcccgt
     2281 taaaaacaga taaattaaaa agccttaaat atgaaaaatc acatttaagg ccttcaaact
     2341 gaaaatttct acgcctcttc ggctttgctg cggataatca aaagcggcag gtggctttgg
     2401 cgcattaccg tttcggcaaa actgcccatt aaaaggtgca tcagcccggt acgtccgtgc
     2461 gtacccaaca ccagcaggtc ggcaccgttt tcatcggcat aatcaaccaa atcctgcgcc
     2521 atttcacgcg cacccttatt ggcaaccagc aggtgtttga cggtattttc cacacccagt
     2581 tcccgggcgg tgcgctcggc ggcatccaaa acttcgttgc cttgcgcgac ggcggcggct
     2641 tcgtagcttt cgtgttgcaa aaattcgggg gcgagtgcca tatattcggc aggattggca
     2701 acgtgcacca aagtcaggcg cgcgctgttg actcctgcaa gttcggcggc atgtttcagg
     2761 gcattgatgg acgtttcact gccgtcaacg gcaacgacca aatgtttgta catatcgtat
     2821 tctccttttg caccgcctcg cggtgccctc ttgtcggatg ggcgcaggga cagtttgcac
     2881 tgtttcatta tagacccgcc gtcgggcttt atacaacagc cgaacagccc gaccgctttc
     2941 cagtataata tgccgcttcc gtgcagtcag gcattttttg ccggctttcg ttcacttttt
     3001 gatttgacgc aatcttgcag gattcgacca tgtccgacaa cgctttgacc tcttcgcgac
     3061 gcttcggcgg catcgccaga ctctacggag actctgcctt ggcgcacttt tcacaggcac
     3121 acgtctgcgt agtcggcgtg ggcggtgtcg gctcgtgggc ggtcgaggct ttggcgcgga
     3181 cgggcatcgg acgtttgact ttgattgatt tggacaacgt tgccgaatcc aatgtcaacc
     3241 gccagctgca cgccctaact ggcgacttcg gcaaagcaaa agttaccgcc ttgcgcgaac
     3301 gcattacaca aatcaatccg caatgcgaag tgtttgaaat tgaagatttc gttaccgaag
     3361 acaatttgcc ggaatacttc ggaaaaggtt ttgatttcgt catcgacgcg atcgaccaag
     3421 tgcgcgtcaa agcggcaatg gcgacttatt ttgtggaacg caaacaaccg tttgtcctca
     3481 gcggcggcgc gggcggacaa aaaaatccgg cgttaatcca aaccgccgat ttgagccgcg
     3541 taacccacga cccgctgctt gccaacctgc gctacacctt gcggaaacgc tacggcttta
     3601 gccgcgatac gaaagccaaa atgcgcgtgc cgtgcgtgtt ctcaaccgaa aacatcacgc
     3661 cgccgcaatc gggcgtggcg tgttctgccg atgccgcgcc gcagggcttg tcgtgcgccg
     3721 gctatggcgc aagtatgctc gttaccgctt cgttcggtct atattgcgcg caggcggcgg
     3781 tggaacacat cgcgggcaaa aaataagcaa tgccgtctga aacaggattc agacggcatt
     3841 tgaacaaact atggttatga tttaagacaa caaaggatac ggataaaaaa taacataaaa
     3901 tatatgattc ctaataatat accaagtatc ggagagctat ttaatggaat tcgttaataa
     3961 tttagttatt ttttcatttt tattactaat gcttattccg atattttttg tagtatatgg
     4021 tatataccat aagatacgtt atcgcaaaat atatatccta agaataagtt ttacattatt
     4081 agtggtaata ctttgcagta tgtattacat atattgccgt tatcttgacc aacaaaaagt
     4141 agcttattat tgcatagatg aacaatgtat ttctattgtt catctataca aagattatgg
     4201 tataaactct cccacatatg cgagaattta cgcaggaaaa atattgttta gatttcaagt
     4261 aagagctaaa aattacgctg aattacttat ggaagatgat atatcaatta gtaaaaaaat
     4321 tttggggaat aaatttatca tttatgggtc gctacctgta atatacggta atgtagataa
     4381 tattgaagta aaagaagcta ctggttatat agatagagcc agtactgatt atattgtctc
     4441 aagaaactta aaattcagac atttatatta attaagaggt tttagcaaga ccgtattcac
     4501 aacctgctcc ttttcaaaac atttgcattt aaaagccgtt ataatgccgt ctgaacatct
     4561 gcccgaccac attatgcgtg aatgccggca gattgttttc ttttgtaaac ttatattaaa
     4621 atccacttac cgattcacgc catgccgccc atccctgccc catccgcacc atccgagcac
     4681 actgtcgcat gggtattcgg ccaacccgtt accgatttgc cccaggattt gtttattccg
     4741 cccgatgcat tgaaagtcgt attgggcagt tttcaaggcc ctttggatct actgctgtat
     4801 ctgatccgca agcagaacat cgatgttctc gatattccga tggtgaagat taccgagcag
     4861 tatctgcact acatcgccca aatagaaacc tatcagtttg atttggcggc ggaatatctt
     4921 ttgatggcag caatgctgat tgaaatcaaa tcgcgcctgc tgctgccgcg taccgaagcc
     4981 gtcgaagacg aagaagccga cccgcgtgcc gagttggtgc gccgcctgtt ggcttatgaa
     5041 cagatgaagc tggcggcaca agggttggat gcgcttcctc gtgcgggccg ggatttcgca
     5101 tgggcgtacc tacctctgga aattgccgtc gaagccaagc tgcccgaagt ctatattacc
     5161 gacttgacgc aagcgtggct gggtattttg tctcgggcaa aacatacgcg cagccacgaa
     5221 gttatcaaag aaaccatctc cgtgcgcgcg caaatgacgg caatcctgcg ccgtttgaac
     5281 ggacacggga tatgcaggtt tcacgacctg ttcaatcccg aacagggcgc ggcttacgtg
     5341 gtcgtcaact tcatctcact gttggagctt gccaaagaag gattggtcag aatcgtgcag
     5401 gaagtcggtt tcggagaaat ccgaatcagc ctcaatcatg agggggcgca ttcagacggc
     5461 atttccggca cacggggcgg gcgcgatgtg ttctaatacg ccccaagccg ccaccaaaaa
     5521 tcgggagaca cgccatatga ccggcatcat acattcgctg cttgacaccg acctctacaa
     5581 attcactatg ctgcaagtgg ttctgcacca gtttccgcag acgcacagcc tttacgaatt
     5641 ccgctgccgc aacgcctcga ccgtctatcc gcttgccgac atcaaggaag acttggaagc
     5701 cgaactcgac gcgctctgcc ggctgcgctt cacccacgac gaactcggct atctgcgctc
     5761 cctgcgtttc atcaaaagcg actttgtcga ttatctcgaa ctcttccagc tccaacgccg
     5821 ctttgtcgaa atcggcacag acgataaaga ccgtctgaac atccgtatcg aaggtccgat
     5881 gatacaggcg atgttttttg aaatcttcat cctcgccatt gtcaacgaac tttacttccg
     5941 ccggctggaa acccctgccg ttatcgaaga aggtgaacgc cggcttcaag ccaaagccgc
     6001 gcgcctcaaa gaaatcgccg ccgcacaaaa ccccgacgat ccgcccttcc tgatttccga
     6061 cttcggcacg cgccgccgct acaagctcgc gtggcaggaa cacgtcatcc gcaccctgct
     6121 cgaagccgcc ccaagcatcg tgcgcggcac cagcaatgtc tttctcgcca aaaaactcgg
     6181 catcaccccc atcggcacca tggcgcacga attcctccaa gccttccagg ccctcgacgt
     6241 acgcctgcgg aatttccaaa aggccgcgct cgaaagctgg gtgcacgaat accggggcga
     6301 tttgggcgtt gccctgaccg acgtggtcgg tatggatgcc ttcctgcgcg atttcgacct
     6361 ctatttcgcc aaacttttcg acgggctgcg ccacgacagc ggcgaccctt acgtttgggg
     6421 cgacaaagcc tacgcccact atcaaaagct caaaatcgac agccgcacca aaatgctgac
     6481 cttctccgac gggctggaca tcgaacgctc ttgggcatta caccaatatt tcaaagaccg
     6541 cttcaaaacc ggcttcggca tcggcaccaa cctcaccaac gatatggggc atacgccttt
     6601 gaatatcgtg ttgaaactgg tcgaatgcaa cgggcagtcc gtcgccaagc tgtccgactc
     6661 tccgggcaaa accatgacca acaacagcac cttcctcgcc tacctgcgcc aagtgttcga
     6721 cgtacccgaa cccgaaacgc cgtaaaccgg cagaaaaagc gcacaattcc tgtttctgcc
     6781 gcataaaatc ttttaaaata ccgcctgatt tgaatttaac cgaaagaccg aacttcatga
     6841 acctacatca aaccgtcgaa cgcgaagccg ccgccgcctt tgccgccgca ggcatcgccg
     6901 acagtcccgt tgttttgcag ccgaccaaaa atgccgaaca cggcgatttc caaatcaacg
     6961 gcgtgatggg tgcggcgaaa aaagccaaac aaaatccgcg cgaattggcg caaaaagtgg
     7021 ctgaagcatt ggcggacaat gccgtgattg aaagcgcgga agtagccggc ccgggcttta
     7081 tcaacctgcg tctgcgccct gatttcctcg cacaaaacat tcagacagcc ttgaacgaca
     7141 cgcgtttcgg tgtggcaaaa accgacaaac cgcaaactgt cgtcatcgac tattcctcgc
     7201 ccaatctggc caaagaaatg cacgtcggcc atctgcgttc cagcatcatc ggcgacagca
     7261 tttcgcgcgt attggaattt atgggcaaca ccgtcatccg ccaaaaccac gtcggcgact
     7321 ggggtacgca gttcggcatg ttggtcgctt atttggtcga gcagcaaaaa gacaatgccg
     7381 cgttcgagct ggcggatttg gagcagtttt accgcgccgc caaagtgcgc tttgacgaag
     7441 cccctgcctt tgccgacacc gcgcgcgaat acgttgtgaa gctgcaaggc ggcgatgaaa
     7501 ccgttttggc attgtggaaa cagtttgtcg atatttcgct ctcgcacgcc caagccgttt
     7561 acgacacgct gggcttgaag ctgcgccccg aagacgtggc gggcgaatcg aaatacaacg
     7621 acgatttgca gcccgtagtc gatgatttgg ttcaaaaagg tctggcagtc gaagacgacg
     7681 gcgcaaaagt cgtgttcttg gacgagttta aaaacaaaga aggcgaaccc gccgcattta
     7741 tcgtgcaaaa acaaggcggc ggcttccttt atgcttccac cgatttggcg tgtctgcgct
     7801 accgtgtcgg cacgcttcat gccgaccgcc tgctgtacgt cgtcgaccac cgccaagccc
     7861 tgcactttga gcaactcttt accacttcac gcaaagccgg ctatctgccg gaaaacgtcg
     7921 gcgcggcatt tgtcggcttc ggcacgatga tgggcaaaga cggcaaaccg ttcaaaacac
     7981 gcagcggcga caccgtgaaa ctggtcgatc tgctgaccga agccgtcgaa cgcgccaccg
     8041 ctttggtgaa agaaaaaaat cccgaattgg gtgcagacga agcggcaaaa ataggcaaaa
     8101 ccgtcggcat cggcgcggtc aaatacgccg acttgagcaa aaaccgcacc agcgattatg
     8161 tgttcgactg ggacgccatg ctctcgtttg aaggcaacac cgccccctat ctgcaatacg
     8221 cctacacccg cgtgcaaagc gtgttccgca aagcaggcga atgggacgcg gacgcgccaa
     8281 ccgttttgac cgaacctttg gaaaaacaac tggccgccga gcttttgaaa tttgaaaacg
     8341 tgctgcaaag cgtggcggac acggcgtatc cgcactacct cgccgcctac ctctatcaaa
     8401 tcgctacctt gttcagccgc ttctacgaag cctgcccgat tttgaaagcc gaaggcgcaa
     8461 gccgcaacag ccgcctgcaa ctggcgaagc tcaccggcga cacgctgaaa caaggcttgg
     8521 atttgctggg catcgatgtg ttggacgtaa tgtaaaaccg caccgcccga ttgcggacaa
     8581 cagccccgca atcaaccggc ggaccatcct tatccgaatc tgaaaaaagc ggcgcgatac
     8641 accgtatccg ccgcctccca aaatgcgaaa cagacaaacg ccaagcaagc aagcaagcaa
     8701 gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaaaa aattataacc
     8761 cccttcctgc cgacgcacgc actttccgcg cggcgcgttc cccttttccc gctcctcaaa
     8821 cccgcctttt cttcaggcag ggtttcagcc cgcctccttt ccctgttttc cttccctcaa
     8881 cacgcgtgcg cgccccctgc cgcaccgtgc cgcactttcg cgcccggacg gcatcggtct
     8941 gtcatccggt tctctgtttt aaatacccct gtttcagaaa gaaattgcag atgtttcaac
     9001 acacaggacg acacataaag caccgcccta tgtgttgccc tgatttggaa ggggttgc
//
LOCUS       DAWFAN010000076         8981 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWFAN010000076 DAWFAN010000000
VERSION     DAWFAN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.076"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(100..297)
                     /locus_tag="WGO65_001252"
     CDS             complement(100..297)
                     /locus_tag="WGO65_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327544.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGITFLNHPF"
     gene            complement(294..1349)
                     /locus_tag="WGO65_001253"
     CDS             complement(294..1349)
                     /locus_tag="WGO65_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0327545.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(1455..1937)
                     /locus_tag="WGO65_001254"
     CDS             complement(1455..1937)
                     /locus_tag="WGO65_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0327546.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(1984..3120)
                     /locus_tag="WGO65_001255"
     CDS             complement(1984..3120)
                     /locus_tag="WGO65_001255"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ0327547.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKSWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTIDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGKPDWFPLQS
                     RPDARLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            complement(3117..3662)
                     /locus_tag="WGO65_001256"
     CDS             complement(3117..3662)
                     /locus_tag="WGO65_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ0327548.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKNREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTHQTGIRANT
                     QTDFSKFKGCLKDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(3659..5134)
                     /locus_tag="WGO65_001257"
     CDS             complement(3659..5134)
                     /locus_tag="WGO65_001257"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ0327549.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTSDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERNDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSNPESEWQETQNKARAVIHAAQMVQEGLDK"
     gene            complement(5193..5354)
                     /locus_tag="WGO65_001258"
     CDS             complement(5193..5354)
                     /locus_tag="WGO65_001258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327550.1"
                     /translation="MPSEALPAGKRARSLKPPPAKCPLRVPDGHLKTRRAASRTLQTA
                     SRSDCHQFR"
     gene            complement(5344..7254)
                     /locus_tag="WGO65_001259"
     CDS             complement(5344..7254)
                     /locus_tag="WGO65_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0327551.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVVLAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSL
                     VFEGNGRLKEYIGGYQDYIDAKSREAKIQTASAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPEIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(7273..7917)
                     /locus_tag="WGO65_001260"
     CDS             complement(7273..7917)
                     /locus_tag="WGO65_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0327552.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(8081..8899)
                     /gene="opc"
                     /locus_tag="WGO65_001261"
                     /gene_synonym="opcA"
     CDS             complement(8081..8899)
                     /gene="opc"
                     /locus_tag="WGO65_001261"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ0327553.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT         1921 a         1975 c         2573 g         2512 t
ORIGIN      
        1 cgctgtactg gtttttgtta atccactata aatgttccga tacgaactgc aaaatattgg
       61 ttttgtttct gacaggcaaa agcactgttt atttggctgt caaaagggat ggttaaggaa
      121 agttatgccc cagataagga tggttgcgcc gacggcttca gacggcattt tggcggcggt
      181 gttgggtttt gtatccggtt tgccgttgtg tcttgtgatg atgattttgg gcgcggtttt
      241 tctgttttga tgtgtgaaat gccgtctgaa aggcggttca gacggcatag cggtcatttt
      301 tgtgcggtca ggcggtcgaa tatgccgccg tcggcgaagt aggttttcat gatgttgtcc
      361 catccgccga atttttcttc gggagagaat gagtctaagt ctgggaagtc ggctttgtgt
      421 cttgccaata cttcggggtt gcgggggcgc aggtagagtg aggcggcgag ttcttgcgcc
      481 ggttcgctcc aaaggtattc gagataggcg cgggcggttt tttgcgtgcc ttttttcgcg
      541 acgacgctgt tgacgacggc gacggggctt tcggcggaaa tggtgtagct cggatagacg
      601 atttcaaact gtccttgggt cagttttttg ctgacgtagt tggcttcgtt ttcaaaagtg
      661 atgagtacgt cgccgatgtt gcgttgtgtg aaggtggtgg tggcggcgcg tccgccgttt
      721 tcaaaaacgg gggtgttttt gaggatggat gcgacgagtt tttgggcttc ctgttcgttg
      781 ccgttggtgg ttttcagacc gtaaccgtat gcgccgagga aggcgtagcg tccgttgccc
      841 gaggttttgg gattggcgat gacgatgtta acgccgtctt tggcaaggtc gttccaatcg
      901 cggatctgtt tggggttgtt ttttcggaca aggaaaacca tagtgctggt gtagggcgcg
      961 gcgtggtcgg ggagggcttg ttgccagcct ttttctacca gtcctttttt ttcgagcagg
     1021 tcgatgtcgg aggattggtt catggttacg acatcggctt gaaggccgtt ggctacggat
     1081 aatgcctgtt tgctggagcc gccgtgggac tgttggatgc tgacggatgt gccggggtgt
     1141 tcggattggt atgttttgat aaataagggg ttgtattctt tgtaaaaatc ccgtgccaca
     1201 tcgtatgagg cgttgagcag ggtaatgttt tttccgtcgg attcggtatt ggccggggca
     1261 ttttgtccgg acggatgatt tgaatcggct gcggggctgc aggcggtgag cagggctgcg
     1321 gtatagagtg ccggtgcgta ggttttcata tgcttgtcct gtcggttggt agatggggca
     1381 actttatacg gctgtctgcg cttgtggaaa taatgtttga tttgaagatt atcagttttg
     1441 gttataagga cggatcagag gtgtttccgc atcagttcgc atttgatttt gatgctgggg
     1501 tcaagctgca atactgccga accgagcgat tcgtagcggt tgagaaggta aaacgggacg
     1561 gagttgagcg atgcgtagag tttcagaaag ctcaaaccgg atttgtgggc gatggtttcc
     1621 gcctgatgaa gtagggcagt gcccagtccg aggttgtgga acagggggtg gacgtaaagc
     1681 gcatcgagtt gtgcttcttg gcagtcgatt tggaaaaatc cctgtatgtt gcctttgtat
     1741 tcggcaaccc aaagtgcttt gtcgggatcg gaaatggtcg gcaggtagct ttctgtgttt
     1801 agcaagcctt cccatacttt tagggcgtgt tcgttgtagc tgaggatgca ggtgtattgg
     1861 acggagtgca ggtggacttt gaagatgtct ttgcagtctt gcacggtggc ggggcgtaac
     1921 agtgtcagca ggttcatggc agtatgtcgg cggcttcaga cggcatctgt gccgttggtc
     1981 ggattatagg gactgatgca gtttttttgc ttcttgaaat gcggtgtccg aatcggtggt
     2041 taaaacggta aagtgtccca ttttccgccc tttgtgcgcg gtttttttgc cgtaaaggtg
     2101 caggcgcgcg tccggccggc tttgcagggg aaaccaatcc ggtttgccgc cgtcttcctg
     2161 ccaaacgtcg cccaaaatat tcgccataca gcaagaactc agcaatttgg tgtcagcagg
     2221 tggcaggttg cacatcaggc ggacctgttg ctggaattgg tctgccgcgc aggcatcgat
     2281 ggtatggtgg ccggagttgt gcggacgcgg cgcgatttcg ttgacgacca attcatgcgt
     2341 gtcgccgaca acaaacattt ctaccgccaa tacgccgacg tagttcaatt catcggccaa
     2401 acgctgcgcc atttgtcgcg cctgttgctg aatgtcggca ctcagtcggg ctgggacgat
     2461 ggagtaggcg aggataccgt tttcgtgaat gttttcggca ggatcgaaag tttgcacgtt
     2521 gtcattgttc agacggcata cgataacgga aatttcgccg cgcaagtcca ccattttttc
     2581 caaaacgcaa tccacgccgc ggtgttcggc aaacgcggct ttgagttcat ccaccgtttt
     2641 gacgcggatt tggcctttgc cgtcatagcc caatgtagcg gttttcagga tgccgggcag
     2701 aaattgtatg ctttcttcag tgatgtcttc ggctttgcaa attgcttgat acggcgcggt
     2761 ttgcaggcct gctttgcgta tccatgactt ttcctgaatg cggttttgcg cgatggcaac
     2821 gcagtcgccg ctgggggaaa cattggtgtg tttggcgaga aaacgcatcg catcggcatt
     2881 gacgttttca aattcagtgg tcaccgccgc gcattttgcc aattcgtcca aagcagcttg
     2941 gtcgttaaac ggcgcgcata aatgacagtc ggcaaattct gctgccggcg cgtctggatc
     3001 ggggtcgaga acggtgactt tgtagcccat ggttttggcg gcaacggtaa acattctgcc
     3061 taattgtccg ccgccaagaa tgccgagcat ggcgggcgga gaaagagata tgtttttcat
     3121 gctgactctt caaattgtac aagttgatag ctataactaa ttcttgacgg atgtcttgta
     3181 tcgctggaat taccagtttc agaaatacag aatacttttt ccataaattt ttctgctttt
     3241 agaaattcca gtattctgtt tttttcatcc ttataagcac cgcggtctgt accccatgca
     3301 agaataatca tatcggcatc tttcaaacat cctttaaatt tggaaaaatc ggtttgggtg
     3361 tttgccctaa ttcctgtttg atgtgtagag taactggaaa aaatatttaa cattttgaag
     3421 ttggtaaaac cgtacatatc caagaaacgt gcaagttggg taagggtttt gtcgcttctt
     3481 tcatcatttg ctttacttgg attaattcct atagcgacag ctgagaaatt tttaggattt
     3541 tctgtgttgc cgctccatct aaccgttagg atttctcgat ttttttcatt atctgtatag
     3601 agaccgtctt tggtagtcgg tctgagtgtt tggcgaagct cataaagttt ttcataagtc
     3661 atttatccaa cccttcctgt accatttgcg cggcgtgtat gacggcacgg gctttgttct
     3721 gcgtttcctg ccattctgat tcaggattgg aatcggcaac cacgcccgcg ccgctttgga
     3781 cgtatagtgt gttgtttttt actacggcgg tgcggatggc gattgccaaa tccatgtcgt
     3841 tgttgaaacc ccatacgccg acggcaccgc cgtagatgcc gcgtttgctc ggttcgactt
     3901 cttcgatgat ttccatggcg cggactttgg gtgcgccgga gagtgtgccg gcagggaagg
     3961 tagcggcgag gatgtccatg ttggtcatgc cgtctttcag acggccttcg acgttggaaa
     4021 cgatgtgcat tacatgggag tatttttcaa tcaccatttt gtcggtaact ttgacttcgc
     4081 cggttttact gatgcggccg acgtcgttgc gtcctaagtc aatcaacatg acgtgttcgg
     4141 cgatttcttt ggcatcgctt aacaaatctt gttcgttggc aaggtcttcg gcgggggttt
     4201 tgccgcgcag gcgcgtgccg gcgatggggc ggacgataac gtcgttgcgt tcgcgtcgga
     4261 cgaggatttc gggcgaggag ccgacgatgt ggaaatcgcc gaaatcgtag taaaagagat
     4321 aaggcgaagg gttgagcgta cgcagggcgc ggtagagggc gagcgggctg tcggtgaatt
     4381 ccatgctcat gcgctggctg gggacaacct gcatgcagtc gcctgcaaag atgtagtctt
     4441 tgattttgtt aacgcaggct ttgaacggct cttcgccgaa ttcgctgacg gcttcggtgt
     4501 gtttgctgcc gagcgagagc gggatggcgc agctttggcg caactgggtg cggatgtctt
     4561 cgaggcgttc gcgggcgcgt tcgtagccgt cgggctgcga cggatcggcg taaacgacga
     4621 gatggatttt gccgctcaaa ttgtcgatca ccgccaattc ttgcgacagc atcagcaaga
     4681 tgtcggacgt gccgagcggg tcggctttgg tggtgttttt caggcggtgg gcgaagtgtt
     4741 cgaaattgta gatggtttcg taaccgaagt agccgaccag tccgccggta aagcgcggca
     4801 ggcttgggat ttcgggtgtt ttgaagcggt tgtggaaggc ttcgataaag ggcagggggt
     4861 tgccgtcgtg ttgctcgaca atttcgccgt tttgataaac atcgacgtgt ttgccgctgg
     4921 ctttgagata gtggctgcaa ggcaggccga taaaagaata gcggccgaaa cgttcgccac
     4981 cgacaacgga ttcgagcagg taggtatagg ggcggttggc gagtttgaga tagagggaaa
     5041 gcggcgtatc caagtcggca aggagttctt gcacgagcgg gatgcggttg tagccttggg
     5101 cggcttgggc ttggtattct tgtttgctga tcatttctgc tttcccaaaa ggcggtttcg
     5161 gacggcgcgg taatgggcgc gagtatagca ttttatcgga attggtgaca gtctgaccga
     5221 gaggccgtct gaagggtgcg gcttgcggcg cggcgtgttt ttaaatgccc gtcaggtacg
     5281 cggagcgggc attttgcagg cggcggcttc aggcttcggg cgcgttttcc cgccggcagg
     5341 gcttcagacg gcattgccgt tttgtttggt ttccagcagc tcccagcgtt ccagcttttc
     5401 caaaagcagc atttcgattt cttcggcgcg gttttgcaat gcgccggctt tttcgtaatc
     5461 tttgaaaatt tcaggatcgg aaagttgcgc gttgatttct gcctgttcgg tttccaaggc
     5521 ggcgatttca tcgggtaggg catcaagttc gcgctgctct ttgtaggaca gtttgaccgt
     5581 gcggttggct ttgggttttt ctttcaccgg ttcggcatcg gctgttttgg gtgcggatgc
     5641 cgtctgaatc ttcgcttcgc gtgattttgc gtcgatgtag tcctgatagc cgccgatata
     5701 ttcttttaac cttccatttc cttcaaaaac aaggctttgg gtaatcacgt tatcgaggaa
     5761 catacggtcg tgcgagacaa ggaataccgt gccttgataa tcgcgcaaca ggtcttcgag
     5821 cagctcttgg gtgtcgatgt ccaagtcatt ggtcggttcg tccaagacca aaatattggc
     5881 gggacgggta aagagttttg ccagcaaaag gcggttgcgt tcgccgccgg agagtgaaga
     5941 aacaggactt tgagcgcggg caggggggaa caggaaatct tccaaatagc tcatcacatg
     6001 ctttttctta ccgccgactt caacgtaatc attgccctgt ccgagcgtgt aaaacacggt
     6061 gtcgttttca ttcaacgcgc tgcggaactg gtcgaaatag gcgacttcct gcttgctgcc
     6121 gatacggatt ctgccgtagg tcggctgcaa ttcgcccaaa atcagtttta aaaatgtggt
     6181 tttgccgatg ccgtttggac cgattaagcc gattttgtcg ccgcgctgca agatggcgga
     6241 gaatttgtcc ataatgactt tgtcgccata agcaaacgag gcgtgttcca gctcggcgat
     6301 gattttgccg cttttctcgc cgctgtcgag cttgaaattg acctgccctt gtacgttgcg
     6361 gcgttcggca cgctggcggc gcagttcttc caaacggcgc acgcggcctt cgttgcgggt
     6421 acggcgcgct tcgatgcctt tgcgtatcca cgcttcttcc tgcgcgtgga atttgtcgaa
     6481 gaggcggttg tgttccgctt cgactgccaa ctcttgcgct tttttctcgc tatatttgga
     6541 gaacgagccg ggataggaac gcaaaatacc gcgatcgagt tcgacaatcc gcgtggcgat
     6601 attgtccaaa aaacggcggt cgtgggtaat cacaaccaag ctgccttcaa acgctttgag
     6661 cagattttcc agccaaataa tcgcgtcgat atccaaatgg ttggtcggct cgtccagcag
     6721 caatacgtcg ggcttttgca cccaagcctg cgccaaaacg acgcgctttt tctgaccgcc
     6781 ggagaggttg ccgatttttt cgttttccgg caaaccgagt tcccccaaag tttgcttgac
     6841 tgccgcatcc agtttccagc cgtccttcgc ttcgatttca agttgcaatt cgttgagctc
     6901 tttcaacaaa gcctcactcg aaccattttc caactcatgg ctaacatgat gataacggcg
     6961 caataaatcg cgaatttcgc ctaaaccttc ggcaacggta tcaaatacgg ttgcgtcctt
     7021 atcaaaaaag gattcctgcg gtacataaac gattttgagg ttgttttgaa caataatctg
     7081 cccgtcgtcg agcttttgca caccggcgag gatttttaaa aacgaagact tgcctgcgcc
     7141 gttgcgtccg atcaagccga ctttttcgcc gctgtcgagt tgaaaagaag ttttgtcgag
     7201 caaggcaacg tggccgatgg caaaagaagc gttttctaca gataatatat tcatgataca
     7261 aattctcaac agttaccgtt tggattttac cgcaagtttg gcgcgggcaa tttcaaccgc
     7321 acccggcagg acggaaacaa taatgatgcc gcccatcacc aagcccagat tgttttttac
     7381 gacggggaag ttggcaaaga aatagcccgc gtaagaaaac aggataaccc acaacaagcc
     7441 accgatgatg ttgtagcgga taaatttggc atagtgcatt ttccccatac cggcgacaaa
     7501 gggggcgaag gtgcggacga tgggtacaaa acgggcaatg atgatggttt tcccgccgtg
     7561 tttttcgtaa aaacggtggg ttttatcgag atattcgcgt cggaagattt tagaatcggg
     7621 attggcgaac agcctgccgc cgaaatattt gccgacggta aaattgagcg cgtcgccgag
     7681 tatggcggca aggcttaata atgcaaccat caaatgaata tccataccgc ccagcgcggc
     7741 aatcccgccg gcggcaaaca gcagcgaatc gccgggcagt aagggcgtaa caatcaggcc
     7801 ggtttcgcaa aaaacaatca aaaacagaat cgcataaatc cacacaccgt attgcgccga
     7861 cagcgcaagc aggtgttggt cgatatggag gatgaagtcg atggcggagg caagcacggc
     7921 gcgttcctaa aaaaaacaaa ccgcgtattt taaccgattg gaaaaatgcc gtctgaaatt
     7981 ttcagacggc atcggctatt caaattcatt tcacgtaaaa accgcaaacc aaaatagttt
     8041 gcggtttggc atttaaagtg acaatgatga tttcaaatca tcagaatttt atgccgacgc
     8101 gcaagccgta ttcacgaata ctggttttcg ggatggtgag cgatacgtcg ccactcttgg
     8161 ttgttacact aaactcgccg gattctttgt aagtgcgttg tttgtagaac ggccccgcct
     8221 cgatgctggc ggattcgccc agttttttac caatatttgc acccaatcca aagccccagc
     8281 cgttggtttt ttgattgatg tcttttttga gattggtaac gccggtgttt aatttatagc
     8341 gggaattgag gtcaaatttc acttcagacc aagggttgat ataccagccg ttacccagtt
     8401 gggaaagcaa atccgcgtga actttggcta accacgactg acggctgctg tgaagcgtat
     8461 gcttggtggt tttaatgctg tcttttgaag attcaaaacc caagccggca cccacacgga
     8521 aatttaaaga atcacttaac gtttgggtgt aggtgtagcc tgtgtaaaga tcgatacggt
     8581 tttcaggaac gccggtgggc agttttacat ttttagtctt acccagcttg ttctcatctg
     8641 tttccaaatt aataatattt tttttgctgc gcccgaaacc ggcttccaag cggatgcctt
     8701 ggttggcatc aaaaggaata tcagcacgta cgccgatgtg tttggcagct ttgtgttttt
     8761 ctttcaggaa agcacgagtt gaagaaatgg aagagaggtc ggtgtggacg gtaaactcat
     8821 tagcggtttg aagctcttgt gcagcggcgg cagtaccggt cagggcaatc atggcacatg
     8881 taaaaactgt ttttttcata gttaaaatct ccaaaatttg gattgtagtc ggatatggta
     8941 acataacgta aataatcgtt acgcttacaa ttatattctt a
//
LOCUS       DAWFAN010000077         8701 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWFAN010000077 DAWFAN010000000
VERSION     DAWFAN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.077"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            191..1726
                     /locus_tag="WGO65_001262"
     CDS             191..1726
                     /locus_tag="WGO65_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0327554.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRRHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1723..1815
                     /locus_tag="WGO65_001263"
     CDS             1723..1815
                     /locus_tag="WGO65_001263"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ0327555.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="WGO65_001264"
     CDS             complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="WGO65_001264"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ0327556.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(3106..3276)
                     /locus_tag="WGO65_001265"
     CDS             complement(3106..3276)
                     /locus_tag="WGO65_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0327557.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3348..3671
                     /gene="cyaY"
                     /locus_tag="WGO65_001266"
     CDS             3348..3671
                     /gene="cyaY"
                     /locus_tag="WGO65_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ0327558.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3698..4714
                     /locus_tag="WGO65_001267"
     CDS             3698..4714
                     /locus_tag="WGO65_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ0327559.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAESVTKAESHKVSVAIAIV
                     VIFGTLAIFTYPLFYTWSQDLINAHQFGIYVGSSVHEVAQVYAIGENINPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSDGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4740..5159
                     /locus_tag="WGO65_001268"
     CDS             4740..5159
                     /locus_tag="WGO65_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ0327560.1"
                     /translation="MAQLPLYRTAEIENFTVGTPEVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAQGEWPKVTDRVLDDELQDKILSRKPT"
     gene            5181..5687
                     /gene="luxS"
                     /locus_tag="WGO65_001269"
     CDS             5181..5687
                     /gene="luxS"
                     /locus_tag="WGO65_001269"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ0327561.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVANVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5833..8628
                     /gene="polA"
                     /locus_tag="WGO65_001270"
     CDS             5833..8628
                     /gene="polA"
                     /locus_tag="WGO65_001270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ0327562.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGLESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESRMHEAADGDLFGSDTIGEQAALDMETSSERLPEKAVAPEKLDYQA
                     VTTEAQFATLLDKLSQAEKIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGHLKPYLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPDMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLVSDDLKSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKVGGGLLD
                     VPLVAEVGVGENWEEAH"
BASE COUNT         2039 a         2252 c         2295 g         2115 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtgttcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaggcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     1861 ttttgatgtt cggcttacag gcaggcgagt tcgtttgcca tttgctgttc caaggtttcg
     1921 cgccggcgga tgagtcggta ttcgttgccg tccaccaaca cctctgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat actggccccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tccccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcgcaaatc
     2101 ggaccgacga tgttggcggt cagaggctca atgtctttgg tttcgaccgc ctcgatgtga
     2161 tgataggcat cataaagcgc cgggcgcatc aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgta tttgacgaat tcgacgcgtg tcagcagcga acctgcgttg
     2281 ccgaccaagc tgcggccggg ctcaagaatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaacggctt gggcatacgc gcccaaatca ggcacttttt cgttttggta aacaatgccg
     2401 acgccgccgc ctaagtccaa atgttccaaa acaatgcctt cggcggcaag cgcgtcaacc
     2461 aaaatcaaaa tgcgttcgca ggcttcgacc agcgggctta agtcggttag ttgggagccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg gcggtaggct
     2581 tcgagcgcgt cggcgtaggc gataccgaat ttgttggctt tcaaacctgt ggagatgtaa
     2641 ggatgggttt tcgcatcgac atcgggattg acgcgcaggg agacgggcgc ggttttaccc
     2701 aaacgcgcgg caactttctg aatacggtcg atttcgggga tgctttccat attgaagcat
     2761 ttcacgcctg cattcagcgc gaactcgatt tccgcctcgc ttttgcctac gcctgaaaat
     2821 atggtttttg ccgcgtcgcc gcctgccgcc aaaacgcgtg ccaattcgcc gccggacaca
     2881 atgtcaaaac cgctccccaa agaagcaaag tgtttgatga tgctcaggtt gccattggct
     2941 ttaaccgcat agcagacgag cggggagagc tcggcaaagg cagtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggtg tgccgaatgc ttcggcaagg
     3061 cgggggtagg ggacttgttc gcaaaatagg gtcatatttt cgttttcatt tttgggttgg
     3121 aggggcggat tgcggtttgc cttgaagctg caaaccggtt tggattacgc cgaagcgcgc
     3181 cttgtcgcct tctttgggca ggtagaggtc gcctttgtag ccgcaggcag agagcagcag
     3241 agccgttgct gctgcaaaaa atacgccgta tttcatcagt aaaactttct tcatcattac
     3301 gaatgtggca agattcggta tcttaaacaa aaaccacaca aaaagccatg atgaccgaaa
     3361 gcgagtttat ccgcgcgagc gaagcattat ttgaacacat cgaagaccaa atcgacgaaa
     3421 acggctggga tttcgactgc cggtttgccg gaaacgtcct gaccatcgaa gccgaaaacg
     3481 gcacgcaaat catcgtcaac cgccacacgc ccaaccaaga attatggatt gccgccaaaa
     3541 gcggtggcta ccatttcgcc gaacaaaacg gcaaatggct ggcaacgcgc gacggacggg
     3601 atttttacga cgttttaaac gaagccctga gcgcggcttc gggcgaagcg gtagagattg
     3661 aggaattgtg atttgggtgt tatcacggaa agaaaaaatg aacacacgtc ccttttattt
     3721 cggacttata tttatcgcga ttattgctat acttgctaac tatttaggaa acactgattt
     3781 ttcccatcat tatcatatca gtgctttaat tattgctatc ttgctgggaa tggcaatcgg
     3841 caataccatt tatccgcaat tttcaacaca agtggaaaaa ggtgttttgt ttgccaaagg
     3901 cgcgcttctt cgcactggca ttgtgttgta tggttttcgc ctcacttttg gcgatattgc
     3961 cgatgtagga ttaaatgcgg ttgtcactga tgcaatcatg ctaatttcaa ccttcttttt
     4021 taccgcactt ttaggcattc gttatctaaa aatggataaa caattggttt atctcactgg
     4081 ggcaggttgc agcatttgcg gtgcggcagc ggttatggcg gcagagtctg ttaccaaagc
     4141 agaatcccat aaagtttcag tggcgattgc catagtggtc attttcggga cgcttgctat
     4201 ttttacttac cccttgttct acacgtggtc acaagattta attaacgccc atcaattcgg
     4261 tatttatgtt ggttctagtg tacacgaagt ggctcaagtg tatgcgattg gggaaaatat
     4321 taatcctatc gtggcgaata ctgccgtcat ttccaaaatg atccgagtga tgatgctcgc
     4381 ccccttttta ttaatgcttt cttggttatt aacacgtagt gatggggtat cagaaaatac
     4441 ctcacacaaa attacaattc cttggtttgc tgtacttttt attggtgttg ccatttttaa
     4501 ttcttttgat ttattaccaa aagaactcgt gaaattattc gttgaaatcg attctttctt
     4561 attaatttca tcaatggctg cgcttggctt aacgacgcaa gcaagcgcaa tcaaaaaggc
     4621 aggattaaaa ccgtttgttt taggaatact aacttattta tggctagtgg ttggtggatt
     4681 tttagtgaac tatggaatat caaaattaat ataaaattca ctaaagagag cgttacccaa
     4741 tggcacaatt acccctgtat cggactgccg aaatcgaaaa ttttactgtc ggcacgcccg
     4801 aagttttaga atcattttcc aaacatatcc cttatggtgt tgtctttgaa gacgacggcg
     4861 acacaggcta cttctatgcc gcttcgcaag acgggatttt agatgccttg cacatttaca
     4921 atgtcgaaga tgtatccgat aaacatatcc ccaatcatgt cttgatttta tgggatgatg
     4981 cctgcaccat agccgcattg tgtatcaacg actacattca tgccgtctat gattttgtcg
     5041 aacaggcagg atattgccgc aacggcttcc ctgaagcaca aggcgaatgg ccgaaagtca
     5101 cagaccgcgt attggacgat gaattgcagg acaaaatcct atcccgaaaa cctacataac
     5161 ctactcaaaa ggataaccca atgcccctac tagacagttt caaagtcgat cacacccgta
     5221 tgcacgcccc cgccgtacgc gtggcgaaaa ccatgaccac gcccaaaggc gacaccatta
     5281 ccgtgtttga cctgcgtttc tgcattccca acaaagaaat cctgcccgaa aaaggcatcc
     5341 acacgctgga gcatttgttc gccggtttta tgcgcgatca cttgaacggc aacggcgtgg
     5401 aaatcatcga catttccccg atgggctgcc gcacaggttt ctacatgagc ctcatcggta
     5461 cgccttccga acagcaggtc gccgatgcgt ggctggcttc gatgcaggat gtggccaatg
     5521 tcaaagacca aagcaaaatc cccgagttga acgaatacca atgcggcact taccaaatgc
     5581 actcgctcgc cgaagcgcaa caaatcgcgc aaaacgtgtt ggcgcgcaaa gtggcggtga
     5641 acaaaaatga agagctgacg ctggatgaag ggctgctgaa cgcctaatcc gccaaaaatg
     5701 ccgtctgaac aagggtttca gacggcattt gccttttccg ttataatctg gggttgtgcg
     5761 ggggcgggtt ttaagccggc atcgtccttc cctatttttt tctgtccctt atcggtttta
     5821 agcgggtttt ttatgtccaa cagacctaca ctcctcctcg ttgacggatc gtcctacctc
     5881 taccgtgcgt atcacgcgat ggggcaaaac ctgaccgcac ccgacggtgc accgacgggt
     5941 gcgctgtatg gcgtattgaa tatgttgcgc cgtttgcggt cggaatatcc gcacgattat
     6001 tgcgcggtgg tttttgatgc gaaaggcaaa aatttccgcc atcaaatgtt tgaagaatac
     6061 aaggcgacgc gcccgccgat gcccgacgat ttgcgtccgc aggcggaagc tttgccggat
     6121 ttggtgcgcc tgacgggctg gccggtattg gtgattgggc aggtggaagc ggacgatgtg
     6181 atcggcacgc tggcaaaaca gggggcggaa catggtttgc gagtcattgt ttcgaccggc
     6241 gataaggaca tggcgcagtt ggtggatgag cgcgttacgc tggtgaacac gatgagcgga
     6301 gaaacgctgg atattgaagg cgtgaaggca aaattcggcg tgcgccccga ccaaatccgc
     6361 gattatctcg cgctgatggg cgacaaggtg gacaacgtgc cgggcgtgga aaaatgtggc
     6421 ccgaaaacgg cagtgaagtg gctggaagcc tacggctcgt tgcaaggcgt gatagaacac
     6481 gcttcggaaa tcaagggcaa agtgggcgag aatctgcgtg ccgccctgcc gcgcctgccg
     6541 ctgtcgtatg atttggtaac gattaaaacc gatgtggact tgcacgccga gctttcagac
     6601 ggcctcgaaa gcctgcgccg taccgcgccg aaatgggcgc agctggttgt cgatttcaaa
     6661 cgctggggct tccgcacctg gctgaaggaa gcggaaagcc gtatgcacga agcggcggac
     6721 ggcgatttgt tcggcagcga tacgataggc gaacaggcgg cgttggatat ggaaacgtcg
     6781 tctgaaaggc tacctgaaaa agccgttgcc cccgaaaagc tggattatca ggccgttacc
     6841 accgaagcgc agtttgccac tttgttagac aaattgtcgc aggcggaaaa aatcggcatc
     6901 gacacggaaa ccacttccct agacgcgatg aacgccgcgc tggtcggcat cagcatcgcg
     6961 ttccaagcgg gcgaagcagt ttacatcccc gtaggacaca gcctgaccgc cgcgccagaa
     7021 cagcttgatt tgcaagacgt attaggccat ctgaaaccgt atttggaaaa tcctgccctg
     7081 aaaaaaatcg ggcaaaacct caaatacgac caacacgttt tcgccaatta cggcatcgcc
     7141 ctgaacggca tcgccggcga cgccatgctc gcttcctaca tcatcgagag ccatctcgga
     7201 cacggcttgg acgaattgtc cgaacgctgg ctcggcttgg aaaccattac ctacgaatcg
     7261 ctgtgcggca aaggcgcgaa gcaaatcggt tttgccgatg tcgccatcgg gcaggcgacc
     7321 gaatacgccg cccaagacgc cgatttcgcc ctgcgcctcg aagcgcacct gcgcgcgcaa
     7381 atggacgaaa aacagcttga aatgtatgaa aaaatggagc tgcccgtcgc gcaggtattg
     7441 tttgaaatgg aacgcaacgg tgtgcaaatc gaccgcgccg aactcgcccg ccaaagcgcg
     7501 gaactcggcg ccgagctgat gaagctcgaa caagaagcct acgccgccgc agggcagccg
     7561 ttcaacctca actcgcccaa acagctgcaa gaaatcctgt tcgacaaaat gggcatcccg
     7621 acaaaagggc tgaaaaaaac cgccaagggc ggcatttcca ccaacgaggc cgtgctcgaa
     7681 cagctcgcgc ccgactaccc cttgcccaaa atcatcctgc aaaaccgcag cctggcgaaa
     7741 ctcaaatcca cctacaccga caaactgccc gacatgattt ctccccggga taaccgcgtg
     7801 cataccacct acgcccaagc cgtcgccatt acaggacgct tggcaagcaa caaccccaac
     7861 ctgcaaaaca tccccatccg caccgccgaa ggccgccgcg tgcgccgcgc ctttaccgcg
     7921 ccaccgggca gcgtcatcgt ttccgccgac tattcccaaa tcgaactgcg catcatggcg
     7981 cacctctctg gagacaaaac cctcattgcc gcattccaaa acggcgaaga cgtacaccgc
     8041 cgcaccgccg ccgaagtgtt cggcactgcg cccgaaaacg tctcgcctga gcaacgccgc
     8101 tacgccaaaa ccatcaactt cggcttaatc tacggtatgg ggcaatacgg ccttgccaaa
     8161 tcattgggca tcgacaacct ttccgccaaa aactttatcg accgctactt cgcccgctac
     8221 cccggcgtcg ccgaatacat gcagcgcacc aaagaacaag ccgccgccca aggcttcgtc
     8281 gaaaccctgt ttggccgccg cctctacctg cccgacatcc gcaacaaaaa cgccaacgcc
     8341 cgcgccggag ccgaacgcgc cgccatcaac gcccccatgc aaggcaccgc ctccgacctt
     8401 atcaaacgcg ccatgataga cgtgtcccgc tggctggttt cagacgacct gaagagcaaa
     8461 ctgattatgc aggtgcatga cgaactggtg ctggaagtcg ttgaaaccga actggatttt
     8521 gtcaaagaaa aactgccgca gattatggcg aaagtgggcg gcggattatt ggatgtaccg
     8581 ctggtggctg aggttggcgt aggggagaat tgggaagagg cacattgatt gaaaggtgtt
     8641 attatgctat ctttatttaa ataaaattta atttttggta tattttttct aaatgttcct
     8701 a
//
LOCUS       DAWFAN010000078         8618 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWFAN010000078 DAWFAN010000000
VERSION     DAWFAN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.078"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            240..1424
                     /gene="coaBC"
                     /locus_tag="WGO65_001271"
     CDS             240..1424
                     /gene="coaBC"
                     /locus_tag="WGO65_001271"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ0327563.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNKSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPIIVANDVSIAMGKPTNQITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1491..3647)
                     /locus_tag="WGO65_001272"
     CDS             complement(1491..3647)
                     /locus_tag="WGO65_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0327564.1"
                     /translation="MPAPQPSAPYDPLTVEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTREMDAVAEGGIAGHWSYKSDSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPIDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDSE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3739..3945)
                     /locus_tag="WGO65_001273"
     CDS             complement(3739..3945)
                     /locus_tag="WGO65_001273"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ0327565.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(4005..4622)
                     /gene="gmk"
                     /locus_tag="WGO65_001274"
     CDS             complement(4005..4622)
                     /gene="gmk"
                     /locus_tag="WGO65_001274"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ0327566.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSQEVIQRRLLKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            4750..5349
                     /locus_tag="WGO65_001275"
     CDS             4750..5349
                     /locus_tag="WGO65_001275"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ0327567.1"
                     /translation="MALKTSNMEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5395..6183)
                     /locus_tag="WGO65_001276"
     CDS             complement(5395..6183)
                     /locus_tag="WGO65_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ0327568.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAATGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDSL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVKREGLKTVRWHEEAVDLLPAGASKMDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6508..7863
                     /gene="yegQ"
                     /locus_tag="WGO65_001277"
     CDS             6508..7863
                     /gene="yegQ"
                     /locus_tag="WGO65_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ0327569.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHDRNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSIAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPGGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7997..8473
                     /locus_tag="WGO65_001278"
     CDS             7997..8473
                     /locus_tag="WGO65_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ0327570.1"
                     /translation="MLAACQSGSAPKNAHTASADTPAGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPIANGRNLVTRQAANSVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
BASE COUNT         2176 a         2354 c         2035 g         2053 t
ORIGIN      
        1 gggatttttt tggggaaatt cgtcatgtcg ctgtcggata aggtttttta tttctgctaa
       61 atactgcacc acctccaata atcccttcct ccccctcctc tgactggtgc gcctttgtga
      121 atatgccgtc tgaaactcgg ggactcagac ggcattttgt atccaaacgg taactaatgt
      181 atccgcactt tgttatagaa tggctgctgt ttttttcttc gtaattagaa attgtcaaaa
      241 tgggtaaaca tattctttta ggtgtaacgg gcagtattgc ggcgtataag tcttgcgagt
      301 tggttcgtct gctgaaaaaa caggggcatt cggttacggt ggttatgagc cgctcggcaa
      361 ctgaatttgt ttctccgctg acttttcagg ctttaagcgg caatcctgtc ctgaccgaca
      421 cgcacggcgg caacggttca aacggtatgg aacatatcaa cctgacccgg aatgcggatg
      481 tttttctgat tgcgccggca agtatgaata ccgtggcaaa aatctgtaac ggcgtggcag
      541 ataacctact gaccagtctg gcagccgcac ggaaatgtcc actcgccatc gctcccgcga
      601 tgaatgtgga aatgtggctc aaccctgcca accaacggaa tatcgcacaa ctggtttcag
      661 acggcattac tgtctatatg ccgggcttag gcgaacaggc ttgcggagaa aatggtatgg
      721 gaaggatgcc ggaacctgcc gaattgctgg atttgcttcc ggacttgtgg acaccgaaaa
      781 ttttaagggg caagaaaatc ttgattaccg caggtgcgac atttgaagcc attgaccctg
      841 tcaggggcat cacaaatacc tccagcggaa aaatgggggt agctttggcg cgggcgtgcc
      901 gtgccgccgg tgcggaagtc agcctgattc acggacagct tcaaaccgcg ctgcctttcg
      961 gcatatccga tacggttcaa gccgtcagcg ctgaagatat gcatcgcgca gtgcatcgtt
     1021 tgattgaaaa gcaagatgct tttatttctg ttgccgccgt ctcagactat aaggttaaaa
     1081 acaagagtac gcaaaaattc aaaaaagata aaaatgccaa accgttatcc atcgaattgg
     1141 atgagaaccc cgatattttg gcttctattg cctcattgcc aaacccgccg ttctgcatcg
     1201 gttttgccgc tgaaacggag aatgtaatgg catatgcacg agaaaaacgt attaagaaaa
     1261 aaataccgat aatagttgcc aatgatgttt caatcgcgat gggcaaaccg accaaccaga
     1321 ttaccattat cggggacgac ggggaactat cttttcccga aacaagtaaa gatgaagcgg
     1381 cgatgcagat tgttgaaagg cttgccgtat atttgaacaa ataagcaatt gaacggataa
     1441 accataaagc gggttgcctg ttaatcaaaa ggcaacccgc tttacctgct tcaacttctg
     1501 atgactttgc ggatatatgg aatactatgc agattttgaa taatctgatt caattgattc
     1561 agattcttga ctttcaataa gaatttgaat tcgacaaaac cttccgttcc cgactgggat
     1621 ttagacggtg tttcgaccga ctcaatgtcc gcaccggaat cggaaatcgc ttgcgccatt
     1681 aatgccaaca ggccgtggct gtcttccgat tggacttgaa gcccgacacg gtagttctgc
     1741 ccattcatat tttcccagtc tgcatccagc tgctgttcgg aatcggactt caacaacgtc
     1801 gggcaggtat ccctatggat aatcatgcct tttcccttaa ccaacagcaa acggatggaa
     1861 tcaccaggaa caggatggca gcactctgca aaatgaatat gcccgctttc ctgaccatcg
     1921 actttaatgg aactgagcct gacctcgctg ccgaaatgct cccctgccaa ctcggcaatg
     1981 tgcatggcga cataaacagg cagggtatgc cccataccga cgttgtacag cacttcctca
     2041 aatgaagtct gtttgtcgtt gagatcggca aggtattttt ctttgatgcc gtctgaaagc
     2101 aggacatctt tgggcagcaa actcgacagg gctttttgta agaggctctc tcccaaaacg
     2161 accgcatcgt gccggttaag gtttttaata tattggcgta tggcactgcg cgccctgccg
     2221 gacacggcga aattcaacca tgcaggattt ggtttggcat gttcggatgt aataatttcg
     2281 acggaatcgc cggttttgag cttcgtacgc aacggcatca tgatattgtt gatacgtgcg
     2341 gcaacggttt tgtgcccaat atcagtatgc accgcataag caaaatcgat aggtgttgca
     2401 cccttgggca atgttaggat ttttcctttt ggcgtaagga tatagatttc gtttggaaac
     2461 aaatctacct tgacgtgttc aagaaactca atagcattgg cactgctggc ctgcaaatct
     2521 aagatatttt tcagccaccg gtttgtgtga agcaccgcct gatcgaccgt cttagaatct
     2581 gatttatagc tccaatgtcc ggcgataccg ccttcggcaa cagcatccat ttcccgcgtg
     2641 cgtatctgaa cttcaatcgg caagccgtaa gggccgacca aagtcgtatg cagactttga
     2701 taaccgttgc ttttcggaat agcgatgtag tctttgaacc gcccgggctt gggctgataa
     2761 agggtgtgca atgcgccgag tgcggcataa caggcgggaa tgctgttgac aatgacgcgg
     2821 aaaccgtaaa tatccataac ctcggcaaag cgcagctttt tcgccatcat tttctgatgg
     2881 atgccgtaca ggtttttttc cctgcctttg attttggcct ctatattcgc gcctaccagc
     2941 ctctgaccga atgcgcgcaa gactttgccg acaacgtcct gccggttctt ccggctcttg
     3001 tccattgctt tttttaaagt ctcgtaacgg ttgggatgaa ggttttggaa cgataaatcc
     3061 tgaagctccc gatacgcatt attcaaacct atacggttgg caatttgcgc gtagatttca
     3121 agggtttccc ttgcaatccg gcggcgtttg tccgggcgca tcgaaccgag tgtccgcata
     3181 ttatgcagac ggtcggcaag tttaacgaca atcacgcgta catctttggt cattgccaaa
     3241 atcagtttgc ggaaactctc cgcctgatgc tctgcatgat cttcaaattt gagtttttca
     3301 agcttggaca gcccgtccac catctcggca accgtattgc cgaacaccgc cgccatttcc
     3361 ccttttgtca cgcccgtatc ttccaatacg tcgtgcatca cgcctgcaca aagaccctgt
     3421 atgtccatat gccaaagggc gagctgcgtc gcaacggcaa tcggatgcgt gatgtagggc
     3481 tccccgcttt tccgggtttg cccgtcgtgg gcacgaaacg cataggcgac agctttttca
     3541 agctccgcct gttcctcggg cttgaggtag gaggcggtat ggaaaagcag ggcacgcgct
     3601 tcgacggtca gggggtcgta aggggcggaa ggttgggggg cgggcatttc agacggcttt
     3661 cggtatgtat gtgtttttca tttcaaaccg tcggactgca cggcggcaaa gtgttccggc
     3721 gtgcgtttcc ggcagaattt atttattgcg cgtcaacagt tctgtaccga tatgtccggc
     3781 ggcgatttcc cttaaggcgg taacggtcgg tttgttattg cggacatcgt ccacaagcgg
     3841 cgtattgcca ttttcaagct ggcgggcccg acgggccgct accaatgtca ggtcaaaatg
     3901 gttggaaatt tttccggtac agtcttcggt ggtaatacgt gccatattat ttgctttctt
     3961 tcaaaaatat ttaaattggg aaaccaggta ttttcgcggt ttttttagga attttccaac
     4021 aaatctgcaa taaatcccag ttgccgcgac cttttcagac ggcaggcatt cacaatatgg
     4081 agcaaatcct cctccgcccg cgccaagtcg tcattgacca cgacaaagtc gaacaatacg
     4141 gactgttcga tttcatgcct tgccttcaac agcctccttt ggataacttc ctgactgtcc
     4201 gtcccgcgtc cgttgaggcg cgcggcaagt acgtcgaaag aaggcggcag gataaagatg
     4261 ccgacggctt ccggcagcgc gtcgcgaacc tgcgccgcgc cctgaacgtc gatttccaaa
     4321 atcacatcat agcctgccgc cgccaacgca ttcacgccct ccgcgcttgt gccgtaatag
     4381 ttgccgaata cgtctgcgta ttccaaaaaa gcttcctgcg cgataagcga ctcaaactct
     4441 tctttggaaa caaagtgata atgtacgccg tttgcttcgc cttcacgcgg cgggcgcgtc
     4501 gtgtgcgaca cggaaacgcg caaaccgtta tggtttgcca atagccgaga aaccagcgtg
     4561 gttttgcccg tgccggaagc ggccgaaatg ataaagatgt tgccttttcg ataagcggac
     4621 atatttttta cctgtatatt ttccagccga ttgtatcaca acggacactc ggtttcctat
     4681 ttgccgatgc ccatattttg ccgctattgt tttgattgat ttggcaagcg gcaggctgac
     4741 ggctacaata tggcgttaaa aacatcaaac atggaacacg caatgctggt tcatcccgaa
     4801 gctatgagtg tcggcgcgct tgccgacaaa atccgcaaaa tcgaaaactg gccgcaaaaa
     4861 ggcatcttat tccacgacat cacgcccgtc ctgcaaagcg cggaatactt ccgccttttg
     4921 gttgatttat tggtttaccg ctatatggat cagaaaatcg acatcgttgc tggtttggac
     4981 gcgcgcggct tcattatcgg cgcggcactc gcctaccatc tcaacgtcgg ttttgtcccc
     5041 atccgcaaaa aaggcaagct gccttttgaa accgtatcgc aaagctacgc gctcgaatac
     5101 ggggaagctg cggtggaaat tcacaccgat gccgtcaaac ccggttcgcg cgtgctgctg
     5161 gtcgatgatt tggttgccac gggcggcacg atgcttgccg gactggagct aatccgcaaa
     5221 ctcggcgggg aaattgtcga agccgccgcc attttggaat ttaccgacct tcaaggcggc
     5281 aagaatatcc gtgcaagcgg cgcgccctta tttaccctac ttcaaaacga aggctgcatg
     5341 aagggctgaa aaccgaccct gccgtctgaa accggcaggg ttgttattat gcgttcaaat
     5401 cacacccaaa tcttgcaagc ccctcaacac gccgtcttca tcaacgccgg ggcaaacata
     5461 tttcgccgct tctttcgccg cctgttcccc gttgcccatt gccacgccga acccgacttc
     5521 tgacagcatt tccacatcgt tcaaaccgtc gccgaacgcc atcacgtccg ccatttccaa
     5581 acccaatgct tcaaccacgc ttctgatacc gtccattttc gacgcacccg caggcagcag
     5641 atcgaccgct tcctcgtgcc agcgcaccgt ttttaagcct tcccgtttca caatatccga
     5701 ccagagcggc atttcgtttt cctccgcgaa caccagcatc tgatacaccg gtttgcttga
     5761 aaaataatcc ttatcggcaa aaaaatcgct ggcgatatgc ttcaaggcgc ggcacacgca
     5821 ttccgacagc gcggacacgg cgatcccctc tccgccgaca aacgcataat ccatacccaa
     5881 gctatccaaa tgcgcgcaaa ccctgcccat caaaccggca tccatcggta cttcgcgcac
     5941 agttttaccg tgcagcagcg caaactgtcc gtttatcgtt accacggcat ccatacccgt
     6001 tgccgccatc atgtccctga ccttttcggg tatcgtcgcc aaagaccgcc ccgttgccaa
     6061 cgccgtcaat atgcctttgc cgcgcaaagc cgccaccgcc gttttcacgg aaggacgcaa
     6121 ggtatccgta tatttgcggt acagcgtatc gtcaatatcg aaaaacacga ttttgggatt
     6181 catcatattc tctctcgcac tcaaactgcc gcattgtatc ccaatcaggc aaatacttga
     6241 taaatcctta taaatttccc ggcaaaattg accgcgaaca cgaaaaggcg gataatccgc
     6301 ccatcttcaa acacccttca gacggcattt gcagcaatgc cgtctgaaac atttttacaa
     6361 agcacacaaa tcatgtttca acacacagga cgacacataa agcgtcgccc tatatgttgc
     6421 cctgattcgg aaagggttac gtccctccca aataaagtct gattctaccg ccctaaaggg
     6481 cggggtttca accgaaaagg aaacacgatg aaagcacccg aacttttatt accggcaggc
     6541 ggtctcgaac gtatgcgcgc cgcctacgat tacggcgcag atgccgttta cgccggcagc
     6601 ccgcgttact ccctgcgcgc ccgcaataac gaatttgcca aactcgacgt tctcgagcaa
     6661 ggcattaaag aagcgcatga tcgcaacaag aaattcttcc ttaccgtcaa tacgctgccg
     6721 cacaactcca aactcaaaac cttcgtttcc gatatggagc ccttgattgc catgaaaccc
     6781 gacgcgctga ttatggcgga tccgggcttg attatgaccg tgcgcgaaaa atggccggaa
     6841 atgccgatcc acctgtccgt acaagccaac accaccaact actggggcgt gaaattctgg
     6901 cagaatatcg gcgtcgaacg catcatcctg tcgcgcgaat tgagtatgga agaaatcgcc
     6961 gaaatccgcc aacaatgccc cgacatcgaa ctcgaagtct tcatccacgg cgcattgtgc
     7021 atcgcctact caggccgctg cctgttgtcg ggctatttca accaccgcga ccccaatcaa
     7081 ggcacctgca ccaactcctg ccgttgggac tacaaagtcc acaacgctac ggaaagtgat
     7141 gcaggcgatg cccagcttct gcaaggtttt aactttgaaa aagcccaaga agaagccaac
     7201 caaaactttg aaggcatcaa cggtcaaaaa cgccatccct acgccgacaa agtcttcctg
     7261 attgaagagt ccaaccgccc gggcgaaatg atgccgatta tggaagacga acacggcacc
     7321 tacatcatga attccaaaga ccttcgcggc attgaagtgg ttgaaaaact cgccaaaatc
     7381 ggcgtggaca gtctcaaagt cgaaggccgc accaagtcgc tttattatgt tgcacgtgtc
     7441 gcccagtcct accgcaaagc gattgatgat gccgttgcag gccgtccgtt tgattacagc
     7501 ctgttgagcg aactcgaagg cctcgccaac cgcggctaca ccagcggctt cctcgaacgc
     7561 caccaaaccc aagactatca aaactacctg accggccact ctattgccaa acaaagccaa
     7621 tacgtcggac acgttaccga aatcgacgaa aacggctggg cgaccatcga agtcaaaaac
     7681 cgcttcgccg tcggcgattc actcgaaatc atccacccgg gcggcaacca aaccatcaaa
     7741 ttggaacaaa tggcccgcaa aggccagcct gtcgatgtcg cacccggcaa cggcattcag
     7801 gtcaaaatcc ccgatatgca gggcaaagaa aaagccctca tcgcacgcgt attgaacccc
     7861 tgagccacaa tgccgtccga accccgtttt cagacggcat tttgttgaat cttacggtac
     7921 aatggcaatg atttcccaca gcaaacaagg acatccgaca tgaaaaaaaa tcctcatact
     7981 ctccgccgcc gccctgatgc tggcagcctg ccaatccggc agcgcaccta aaaacgcgca
     8041 cacggcatcc gccgacacac cggccggaga atcatgcaaa gtcatccggg acgacaccgc
     8101 aggaagcggc aaaaacatca tttaccgttg ctgcaacggt caggcggcaa taaatgcagc
     8161 cattgcagac ggcgtattgg aatcagggat tcccgtcagc ttcggcggcg ggggcgtacc
     8221 gattgcaaac ggcaggaatc tggttacacg gcaagcagca aacagcgttg gcaaatccga
     8281 ctccgaggcc tgcgaacacg ccctaatcaa tgccgcccgc aaattccagc agaccgccgg
     8341 caaactcggc gggcgcagtg ttaccggctt ccacagctac ttcgacaagc aaccgctgca
     8401 aggaggacaa tacgactgtc aggcaggctc gttccacgtc cgcgtggtca tgcgcgccaa
     8461 tgtcgtccgt taacatatcg gcgaccaaaa aattaggctg tgttttagta atctgttgat
     8521 ttcaattatt tgcaaaggaa aagacaatta ttttttcggt taggaagaga cctatcctat
     8581 tgaatacatt taagttaaat acgtctatca acactata
//
LOCUS       DAWFAN010000079         8575 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWFAN010000079 DAWFAN010000000
VERSION     DAWFAN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.079"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(111..938)
                     /gene="fghA"
                     /locus_tag="WGO65_001279"
     CDS             complement(111..938)
                     /gene="fghA"
                     /locus_tag="WGO65_001279"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ0327571.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(947..2056)
                     /locus_tag="WGO65_001280"
     CDS             complement(947..2056)
                     /locus_tag="WGO65_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ0327572.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFELMHEGKSIRAVIHY"
     gene            2204..2611
                     /locus_tag="WGO65_001281"
     CDS             2204..2611
                     /locus_tag="WGO65_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ0327573.1"
                     /translation="MAYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(2811..3125)
                     /locus_tag="WGO65_001282"
     CDS             complement(2811..3125)
                     /locus_tag="WGO65_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ0327574.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(3136..4821)
                     /gene="rpsA"
                     /locus_tag="WGO65_001283"
     CDS             complement(3136..4821)
                     /gene="rpsA"
                     /locus_tag="WGO65_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ0327575.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQDKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDESRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(4977..5633)
                     /locus_tag="WGO65_001284"
     CDS             complement(4977..5633)
                     /locus_tag="WGO65_001284"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ0327576.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            complement(5877..6922)
                     /locus_tag="WGO65_001285"
                     /pseudo
     CDS             complement(5877..6922)
                     /locus_tag="WGO65_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(7059..7307)
                     /locus_tag="WGO65_001286"
     CDS             complement(7059..7307)
                     /locus_tag="WGO65_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0327577.1"
                     /translation="MKRHPTLQPLSRQHHLGLVIANKAKSVTDDDKLTQHQAFILTVA
                     GATVGFGATWRFPYLVGENGGARMCFYSVSRCWLSASR"
     gene            complement(7401..8564)
                     /locus_tag="WGO65_001287"
     CDS             complement(7401..8564)
                     /locus_tag="WGO65_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0327578.1"
                     /translation="MQRKFDLAQKPVLLSYGIDYEQEKNRQGATGYDLTTFMQSNGLA
                     FKDTGNRYTYGPDTTLKTYGIFANANVALTDDLKISAGVRHQIIDSKTDGFVPPSESL
                     LADLLNQNKVPYQAGTVNSGKVKHHKTLFNLGANYSLTPNHSIFANFGQGYSLPDLQR
                     VLRDVGTGFAVNSDNVEPISVNSYDAGWVGVFGGSRAKLTGFYNTSDKVTQFLKDYSV
                     VVADTDERIYGAEFELNHKINDNWAVGGSIAYTRGQYKDASGQYRELGAFRISPTKAT
                     AFAQYTTPSEHKLRLQLLAIDGTQRAYKDSLIAKTNQKVRKTPEARISGYVVADLLGE
                     IKLPKGQLNFGIYNLTNKKYKSVFSQAAKVTYGELSSLSAQGRSYGISYTLNY"
BASE COUNT         2152 a         2051 c         2030 g         2342 t
ORIGIN      
        1 ccgtagagtc ggctccttcc ggctcagacg gcatacgttt atgccggcac ttcttaccgc
       61 cctcgtgccg gctatcccaa aactgctttg aacactgaac gctctttggt ttacttcaaa
      121 aacgcggcgt gataagcaat atgctcgcca ataaaactgg cgatgaagta atagctgtga
      181 tcgtagcctt tatggaaacg cacatcgacc ggctggtttg ccgcacggca ggtttcgata
      241 aaatcttcgg tacgcaattg tgtcggcaaa aactcatctt ccaagccttg atcgatgcgc
      301 ataccttgca ctttatagcc ttgttgaatg agtgagttcg catcatattg ctgccatttt
      361 tcacggtctt tccctaaata agcagtaaag gctttttctc cccacggcac gaggcttggc
      421 gataaaatag gcgaaaaggc agaaacactt tgataacgtt cctgattccg cagcgccaat
      481 accaatgcgc cgtgtccgcc cattgaatgc cccataatgg aacgtttgcc gttggtagga
      541 aagtgtttct caatcagacg gggcagctcg ttcaaaatgt aatcatacat ttgataattc
      601 gccgcccaag gctgttcggt cgcattcaaa taaaagcctg caccctgtcc taaatcgtaa
      661 gcatcatcgt tcggcacttg ctctccgcga gggctggtat cggaggccac cacaattact
      721 tgatgttctg ccgcataacg ctgaaagcct gatttggtaa tgaaattttg ttccgtacac
      781 gtcaagccgg aaagccaata aatcacacca agcggtcgat tttctggatt atttggcaaa
      841 tagacggcaa atttcatttc gcattgcagc gtttgggcat gatgcgccca aacttgttgc
      901 gaaccgccaa aaatttgatg ttgttcaatc agtttcatcg cataccttag tagtgaataa
      961 cggcgcggat tgatttacct tcgtgcatta actcaaaggc ttcattgatt tgatcgagtg
     1021 tcattgtgtg ggttacaaac ggttctaact caatgtcgcc tttcattgaa tcttccacca
     1081 ttttcggcag ttcagagcga cctttcacac cgccaaatgc tgaacctttc caaacacgac
     1141 ctgttaccaa ctggaacgga cgcgttgaaa tttcttgtcc tgcacctgct acgccgatga
     1201 taatggattg tccccaacca cggtgcgcac tttctaatgc ctgacgcatt acgtttacgt
     1261 tgccgataca ttcaaaggtg tgatcaatgc cccatttatt gatgtctaac aacacatctt
     1321 taatcggttt atcgtaatcg ttcgggttca aacaatccgt tgcaccgaac tgttttgcca
     1381 actcaaattt tgatggattg gtatcaatgg cgataatgcg gccggctttg gcttgacgcg
     1441 caccttgcac caccgccaaa ccaatcgccc ccaaaccaaa cacggcaaca gagtcgcctt
     1501 cttgcacttt tgccgtatta tgtaccgcac caatacctgt ggtaacgccg cagccgagca
     1561 aacatacttg ttcatggttg gcttcagggt tgattttcgc cagtgaaact tcggcaacaa
     1621 cggagtattc actgaaagtc gaacagccca tatagtgata aatcggctga ccttgataag
     1681 aaaaacgcgt cgtgccgtcc ggcattaagc ctttaccttg tgtatcacgc actgagacgc
     1741 acaagttggt tttacctgaa caacaaaact cacattcgcc acattcggcg gtgtaaagcg
     1801 gaatcacgtg atcacccggt tttacgcttg acacaccttc gcccacagca acgaccacac
     1861 ccgcaccttc gtgtccaagc accacaggga atacgccttc aggatcgctt cctgataacg
     1921 taaacgcatc agtatggcac acgccagtgt gggtattgcg gattaacacc tcgcctttac
     1981 gcggcatttc tacgtcgatt tccacaattt gtaagggttg gttaggggcg aatgccaccg
     2041 ccgcacgaga tttgatggtt gaatcggttt gtttcatttc cattgtcttt cctcttaatt
     2101 cattgacaaa gttgccttac ctgagcaaca tagcaacagt ttacacttta gagttaactc
     2161 taaagcaagt gttttttact gtttttgttt agaaggaggg aaaatggctt ataccactgc
     2221 caaagccgcc gaaaaaatag gcatctccgc ccacacccta cgtttttacg acaaagaagg
     2281 cttgttgcct aatatcggac gtgatgaata cggtaaccgc tgttttaccg ataacgattt
     2341 gcaatggttg ggcttattgc aatgcttgaa aaatacgggg atgagcttaa aagacatcaa
     2401 acgctttgcg gaatgtaccg tcattggcga cgataccatt gaagaacgcc tttccttgtt
     2461 tgaaaatcaa atagaaaatg tgaagtgtca aattgccgaa ttaaaacgct atttagattt
     2521 gcttgaatac aaattggcgt tttaccaaaa agcgaaagca ttaggctcgg taaaagctgt
     2581 aaatttgccg caaattcctg aaacggctta gttttagtgc aataagcagg atttttgtcc
     2641 tgttcgtaat agaaatcaaa aatttgttgt ttaaaaagcg gattgtaagc aatgataaaa
     2701 aaatccacgc tctaatccgt tgcttgcttg tcttaaggta ttgaagcagc ggatgaagtt
     2761 aaaccacaga aaaaaaacgc cgcttggaag cggcgttttg atgctaaggt ttaattggca
     2821 ttttctttta aagccaaatc gacccgctcg cgcaattctt taccgggctt gaagtggggt
     2881 acatgttttt caggtacttc cacacgctcg ccggttttgg gattgcgacc gatgcgggca
     2941 ggacgatggt tcaaatcgaa gctgccgaaa ccgcggattt cgatgcgttg acctcgggca
     3001 agcgatctag tcatggtgtc aaccaagact tttacgctgt actctacgtc ttttgccaga
     3061 agatgcgtgc cgtttttggc ggcaaacact tctgccaaac gaaccattaa ctcagacttt
     3121 gtcatgtctg caaccttatt cttgttcgcc ggagagtttg gctttcagca ggtcgcccaa
     3181 gctggtggtg ccggcattcg cattggcggc ggcattgacg gagttcagtg cttcgcggct
     3241 ttctttggca tctttggctt taacggaaag tttgatgctg cggtttttgc ggtcaacggt
     3301 aacgatgacg gcttcaactt cgtcgccttc tttcagtttg gtggtcaaat cttcaacgcg
     3361 gtcggctgca aattcggaag caggcaggta gccttctact tcgtcagaca gggcgataac
     3421 agcacctttg gcgtcaacag atttcacgga acctttaacc aaagaacctt tgtcgttcac
     3481 gctgatgaag ttgccgaacg gatcgccttc cagttgtttg atacccaagg agatgcgttc
     3541 tttttccacg tcgattgcca atacgacggc ttcgacttct tcgccttttt tgtatttgcg
     3601 tacggcttct tcgccggatt cggtccagga caggtcggac aggtgaacca aaccgtcgat
     3661 gccgccaggc agaccgacga atacgccgaa atcggtaatg gatttaaccg cgccggagat
     3721 tttgtcgcct ttgttgtggt tggcggcaaa ttcttcccaa ggattggctt ggcattgttt
     3781 catacccaaa gagatacgcc ggcggctttc atcgatttcg aggatcatta cttcgacttc
     3841 gtcgcccaat tgtacaactt tgctcgggtg tacatttttg ttggtccaat ccatttcgga
     3901 aacgtgcacc aagccttcga tgccttgttc gatttcaacg aacgcaccgt agtcggtcag
     3961 gttggatact ttaccgaaca ggcgggtacc ttgcgggtaa cgacgggtca ggccgctcca
     4021 aggatcttcg cccagttgtt tcatacccag agaaacgcgt tgtttgtctt ggtcgaattt
     4081 caggacttta gcttctactt cttgaccgac ttccagaact tcgctcgggt gtttcacacg
     4141 gcgccatgcc aagtcggtga tgtgcagcag accatcgatg ccgcccaagt caacgaatgc
     4201 accgtaatcg gtaatgtttt taacgatgcc tttgatgacg gagccttctt gcaggttttc
     4261 cagcagggct ttgcgttctt cacccaaggt agcttccaga acggcgcggc gggacacaac
     4321 aacgttgttg cgttttttgt ccagtttgat cactttgaat tcaatttctt taccttcgaa
     4381 gtgagaagtg tcttttacag gacgtacgtc gaccaaagaa cccggcagga atgcgcggat
     4441 gctgctaatc ataacggtca ggccgccttt gactttgccg ttgatgatgc cggacaggat
     4501 gttgccgttt tccatggctt cttccagggc aatccaatcg gcagcgcgtt tggctttttc
     4561 gcgggacagt ttggtttcgc cgaagccgtt ttcgacggat tcgatggtaa cggtaacgaa
     4621 gtcgccgact ttaacttcaa tttcgccttg agcgtttttg aattcagcta catcaatcag
     4681 ggattctgat ttcagacctg cgtttacggt aacgaagttt tggtcgattg ccactacttc
     4741 agcggtaatc acctcacccg ggttcatttc ttgcagggta aagctttctt ccaacagctg
     4801 agcaaaattt tccatagaca tatataactc ttttcggtac accgccaagg ggtgcggggt
     4861 gggttggtgg atatctgccg tccttggcag ggcagacggg tacataaggt ttcagacggc
     4921 atatggggga tttttatgcc gtctgaaacg taatttgtgc gattatacct gaaaatttaa
     4981 acttcacgat accaatcaag cacttttttt acagtttctt ctatagtcag gcggcttgtg
     5041 tccaaaagca gggcatcggg ctgttgtttc aggggggcaa ctttgcggtt tcggtctgcc
     5101 tcgtctctgg cttcgatgtc ggacaggatg cgctcgaatg ccaaaccttc gcaggggatg
     5161 ccgatttgtt tggcgcggcg ttcggcacgg attttggatt ctgccgtcag gaagatttta
     5221 agttcggctt gggggaagac gaccgatccg gtgtcccgtc cgtcggcaac cagtcctttt
     5281 tcggtcagaa aatcgcgttg gcgttgcagc agggcggcgc ggactttagg caactgtgcg
     5341 actgcggatg cgcccatgcc gatggcttct gtccggatgc cgtctgaaac gtcttcgccg
     5401 ccgagcagga tgcggctgcc tgaaaatacg gcgggcagtt tttttgccag ttcggaaacg
     5461 ttttcttcat cgtgccatcc cacgccttgt ttttgtgcat atagggcagt caggcggtag
     5521 agtgcgccgg tatcgagata atcgtatccc aatgcggcgg caacgcgggc ggcaaccgtg
     5581 cctttgcccg atgcgcccgg gccgtcgatg gcgatgactt tttgtctgtt cataaggggg
     5641 attcctgatg gtttggggta tgggttttgc cgtctgaagg atgtgtttcc cgttggggcg
     5701 gattctacct gttttaaagg acgattgtct aagcagacgg aacgccgcct gcccgaaaac
     5761 atccgacaga agccggcaag ccggttcgga ttccggcgtt gcgggtattg tggcgggcat
     5821 caaggcaatg ccgtctgaaa gaggtatggc cttcagacgg catcgattgc tgcagattag
     5881 aacaggttgc tgacaaaaat cagcaaaatc accaacggca caagatattt cacataagca
     5941 aaccaaatat tgaccgtcgt atggttgcct ttgtaaagca attcttcctt cgcttcgtcc
     6001 ttcatcacaa aaccgacaaa cagcgcgaaa ccgagcgcgg tcagcataaa caagatgttg
     6061 ccgctgatgt agtcgaaggc atcgaaaata tttttgccga acacggaacg tctttccacg
     6121 aaccatagct cagaatggac gggatgttgc cgaaaatgaa gatggcggct aatacaatcg
     6181 taatcgcggc ggtacggcgg attttggttt tttcctgaat ggtcgtaatc aacacttcat
     6241 aaatggtcag cgaagtcgtc aatgcggcaa tcaggagcag cgagaagaaa atcacggcga
     6301 acacagatcc tgcccacata tgcgagaaca caatcggcaa actttggaat accaaagtcg
     6361 ggccggaatc gggggcaacg ccgaagctga agagcgacgg gaaaatcata aagccggcca
     6421 gtacggcgat gatggtattg gtaatggccg tgatgaccgc ggtttgcacc agattttcgt
     6481 tcttatccag atagctggac aaggtaatca tcacgccgaa acccaagctc aaggtaaaga
     6541 acacttgtcc taaaacgaag acgaacagtt cggctgtaat tttgctgaag tcaggtttca
     6601 gatagaaggt aatgccttcc attgcgcccg gcagggtaac gttgcgcaca accatggcaa
     6661 tcaggaataa aaacagcaaa ggcatcaggt attttgctgc tttttcaata ccgccgatca
     6721 cgccttttac caagatccat tggttcacaa ccacaaatag gaaggtgtag aaagcaattt
     6781 cccaaggact gttttcgatg tgttcggtaa agaaactttt ggtgacttcg ctggaaacag
     6841 gactggaaat gtccaaatta ccgccgatga tattaacaat gtagctgata acccagccgc
     6901 cgagcaccat gtaataagcc atgatgccga acgcgccgag cagcccatcc agccgaccag
     6961 tttccaaatt ttggcaatgg gtttgccgtt catcgggccg ccgaacgcat ccagcgcgtt
     7021 cacgcctttg cgccgtccga tgacattttc caccaaaatc atcgggatgc cgataaccag
     7081 catcgcgata cagaataaaa acacatacgc gcaccaccgt tttcaccgac caaatacggg
     7141 aaacgccacg tcgcgccgaa accgacagtc gcgccggcaa cggtcaggat aaaggcttga
     7201 tgttgggtaa gtttgtcatc atcggtgacg gatttggctt tgttggcgat gaccaagccc
     7261 aaatggtgct ggcgagatag gggctgtaag gtggggtgtc gtttcatggg ggttcctgtt
     7321 ggtaaattaa attttattat aaaataaaaa aaccacaaac actagggcga tgtttgtggt
     7381 attgtggcac ataatgctga tcaataattt aaggtataac ttatcccata acttctcccc
     7441 tgtgcagaca agctggataa ttcaccataa gtcactttgg cagcttgact aaatacactt
     7501 ttatattttt tatttgtcaa gttataaatc ccaaagttta actgaccttt aggtagtttg
     7561 atctcaccca ataaatccgc cacgacatag cctgaaattc tagcctccgg ggttttgcgg
     7621 accttttgat tggttttggc aatcaggctg tccttgtaag ctctttgtgt accatcaatg
     7681 gcaagtagtt gtaagcgtaa cttatgttca cttggtgtgg tgtattgagc aaaagcggtg
     7741 gctttggtag ggctgatacg aaacgcccca agttctcgat attgaccgct tgcatctttg
     7801 tattgacctc gtgtataggc aatgctacca ccgacagccc agttatcatt aatcttgtga
     7861 tttaattcaa actccgcccc ataaatacgc tcatcagtat cagcaaccac cacgctataa
     7921 tcttttaaaa actgggttac tttatctgat gtattataaa aacctgtcaa cttggcacgg
     7981 ctaccgccaa acactcctac ccaccctgca tcataactat tgactgaaat tggctcaaca
     8041 ttatcagaat taaccgcaaa acccgtccca acatctctta gcaccctttg taaatcaggc
     8101 aaactatatc cttgcccaaa attggcaaaa atactgtgat ttggagttaa tgagtaattt
     8161 gctccaagat taaataaggt tttgtgatgc ttcactttgc cagaattgac agttcctgct
     8221 tgatacggca ctttattctg gtttaataaa tcggctaata gcgactcact gggtggcaca
     8281 aaaccatctg ttttactgtc tataatttga tgccgtacac ctgccgatat ttttaaatca
     8341 tctgtcaaag caacattagc attggcaaat atgccgtagg tttttaaggt ggtgtcaggt
     8401 ccataagtgt aacggttgcc agtgtcttta aatgccaagc cgttgctttg cataaaggta
     8461 gtcaaatcat agcctgtcgc cccttggcgg tttttttctt gttcataatc gataccataa
     8521 ctaagtagta caggtttttg agcaaggtca aattttcttt gcaaagccag tcttg
//
LOCUS       DAWFAN010000080         8273 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWFAN010000080 DAWFAN010000000
VERSION     DAWFAN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.080"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(244..861)
                     /gene="thiE"
                     /locus_tag="WGO65_001288"
     CDS             complement(244..861)
                     /gene="thiE"
                     /locus_tag="WGO65_001288"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ0327579.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIALCVAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLREYVKQARGTPVVA
                     IGGIDLNNAQAVLATGVSSLAVVRAVTEAANPEAVVKAFQALWDG"
     gene            complement(882..1982)
                     /locus_tag="WGO65_001289"
     CDS             complement(882..1982)
                     /locus_tag="WGO65_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0327580.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNQAPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHIFVVGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            complement(1979..3202)
                     /gene="cytX"
                     /locus_tag="WGO65_001290"
     CDS             complement(1979..3202)
                     /gene="cytX"
                     /locus_tag="WGO65_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ0327581.1"
                     /translation="MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     regulatory      complement(3268..3371)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3446..4930
                     /gene="tldD"
                     /locus_tag="WGO65_001291"
     CDS             3446..4930
                     /gene="tldD"
                     /locus_tag="WGO65_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ0327582.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILTGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     gene            4995..5456
                     /locus_tag="WGO65_001292"
     CDS             4995..5456
                     /locus_tag="WGO65_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327583.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAASAADGIKNDV"
     gene            5446..6267
                     /gene="prmC"
                     /locus_tag="WGO65_001293"
     CDS             5446..6267
                     /gene="prmC"
                     /locus_tag="WGO65_001293"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0327584.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            6353..7741
                     /locus_tag="WGO65_001294"
     CDS             6353..7741
                     /locus_tag="WGO65_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ0327585.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLTGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            7742..7966
                     /locus_tag="WGO65_001295"
     CDS             7742..7966
                     /locus_tag="WGO65_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ0327586.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
BASE COUNT         1750 a         2237 c         2516 g         1770 t
ORIGIN      
        1 ctagccgaat tactttattt tttgatacgc aaccggccgg ttgccgtcat tcccgcgcag
       61 gcgggaatcc atttttgaaa ttcggaaact gtttttcaaa tgatggtttc tcaagtttta
      121 aggcggattc ccgcttgcgc ggaaatgacg gaatttgatg atatgccaca tttaaagtta
      181 agtatgtttg cgataacggg ctaagttttg cctacacggc aattgaattt tcttctttcg
      241 gttttatcca tcccacaaag cctgaaacgc tttaaccacg gcttcgggat ttgccgcttc
      301 ggtcacggca cggacgacgg cgagtgagga tacgcctgtt gccagcacgg cttgggcgtt
      361 gttcaaatcg atgccgccga tggcgacgac gggcgtgccg cgagcctgtt tgacgtattc
      421 gcgcagcttg tccaagcctt gcggggcggt gggcatttgt ttggtcgtgg tcgggaaaat
      481 cgcgccgctg gcgacgtagc tggggtgcac ggacagggcg cggtcgagtt cggcgacgga
      541 gtgcgtactc aaacccaagc gcaaaccggc agcggcaatg gcggcgaggt cggcggtgtc
      601 catgtcttct tgtccgagat gcacgccgta cgcgcctgct tcgattgcct cgcgccagtg
      661 gtcattgatg aaaagctgcg tatggctgcc ctgacaggcg gcgacacaga gggcgatttc
      721 gcgtttcaat tcatcgccgt gcagggcctt gcagcgcagt tgcaccgtgt cggcacctgc
      781 tttgaccatg cgccccaccc aatcggcggt ggggacgacg gcgtagaatt tgagcgggga
      841 tttgagtggc gggaaggtca tggggttcct ttcagacggc tttaatcttg tcttcggata
      901 tacgccaaac cgctttcttc atcacgttcg ggcgcgtctt ttccgtcaaa cagtgccact
      961 gccaatctga cggcggcggc ggttacggcg ggggagatca tgaaaccgtg gcggaaaagg
     1021 ccgttgattt cgatgaggcg gcgtttgcgg ctgtagcgga tttcggggtt gtggtggttg
     1081 agcgtggggc gcaggccggt ggcgatttcg aggatgtcgg cttcgccgaa ggcaggatgg
     1141 acggcataaa gcgcggataa gagttccagc ccggaacgta cgctggcggg ggcttggctt
     1201 tcgctttcga tttgggtcgc gccgacgacg aaaatgtggt tttctttcgg agcgatgtag
     1261 agcggatagc gcggatgcag cagacgcacg gggcggttga gcgtgatttc gggcgtataa
     1321 acccgcgcca cttcgccgcg tatgccgcgc agggtgctgg tgtgctcggg ggcttggttc
     1381 caagcggttt ttgcgccgta gccgcggcag tcgatcagcc agtcgtattg ggcttgcagg
     1441 tcttcggggg cacattcgtg ttcccaatgg caagggacgt tcagttcgtc caaagcgtcg
     1501 gcaagtgcag acaatatttg ccgcccgtcg agctggcctt cggtcggcag gtagatgccg
     1561 tctgaaaaac gtccgccgag ttgcggttcg cgttcggcga tgtcgtcggc gcgccaacgg
     1621 acgatttcgt catccgctac gccgccgcgt ttgagatggc ggacgaactc gctggataat
     1681 ggcttgtcct gcccgtgcca cacaatcagg ctgccgtttt cctgcatcat cgtgtgcgtg
     1741 ttcagacggc atcggatgcc gcgccaaagc gggatgctct gcctgcccag cctgaccact
     1801 tcgggcgtgg cttcgaccgc ttccgccgca ggcgcgagca tggcggcggc aacataggcg
     1861 gcggcgtgtt cgccccggcg gcagccttta tcgaaaagtg caatctgata accttgttct
     1921 gcaagctgca gtgcggtcag ccttcccgag aggccgccgc cgaggatggc gatacgggtc
     1981 atgacgggtt cctttgtaaa gattgggttt ttttaaagaa aaggcgtacc gatacggtgg
     2041 caatggcaac ggcagacatt acgggggcgg tcagaccgat gctgctttcc cagccggacg
     2101 agagcaggaa gcggtagagg atgaagcccg ccagccacag aaccagtccg gcaaagtcaa
     2161 agccttcaat ctcctcacgc cgtttcaaga cgaaaaagtc ggcaatcaaa accgccgcca
     2221 tcggcgcaaa taccgagccg ataagcagca ggaagttttc atattcggta acgggcagca
     2281 tgacggcaag caccgtgccg atcagggtaa cgccgacagc gacgggtgtt tccgcaaaac
     2341 gcgcggaaat gttgttcgca ctcgcgccgg cggaataggc atcgagaaaa gtggtggtaa
     2401 cggtcgacag gacgaccgcc aaaatgcctg ccgcacccaa acctgcgccc agcaggattt
     2461 ttgccacgtc ggtttctccg gtgaacaacg ccgctgccaa acccaaggca tacatccagc
     2521 agcccgtcag cgtataggcg agcgttgccg tcagggttgc cgcaaacggg cggcgtgctt
     2581 ggcgtgtgta gtcggcggcc agcggcagcc aggaaagcgg catgacggcg gacaattcga
     2641 ctgccgttcc gaaactcatg ccgtctgaaa cctgtgcggc ggtgctgcct gccgtggaaa
     2701 agacttcggc actcagccac agaaccgcca acagcatcag cagcatcgaa acggttttca
     2761 gcccgcctgt tttgcgtgcg ccgaaaacca gccacagcac aatcagcgcg ccgtttgcca
     2821 atgcccacca gacaaaagat tcgccgtccc acaacacttt gcccaaagcg gagctgaccg
     2881 ttgcgccggc gtaaatcatc accgccgtcc agccggccag ttgcagcata ttcgccacgg
     2941 aaaacagcac tgaaccgcgt ttgccgaacg acaggcgcac gctttccatc gagctgcgtc
     3001 cggtcagtgc gccgatatac gccgccgcaa aaaacagcgc gccgccgacg gcatgaccca
     3061 aaagtagagc cgccagaccg cgttgccagc ccaaaggcgc aagcagcgta cccgtgctga
     3121 tttcggcaat cgataccgcc gcgccgaacc aaatcagccc gatggcggag gaagatgaag
     3181 gagaggaggc attgcccgac atatcaagtc cttaaaaaac atgaaaatgg ggcggggaag
     3241 tgcggaaaag ggaacggaag cccgaaaggg cggggaggtt ttgtttccta cgcaggcatt
     3301 accccgacag gttctacgga ttctgctttc gcaatctcag ccgcccggcg gcggcactcc
     3361 gacaaggagc tttaagtgta cacaaaagca gggggcgttg gcaaaaatct tacatttcag
     3421 gcggcacggc gcatttccat aacaaatgcc gtataatcaa gcccctgatt ttttatgcgc
     3481 cgacactatg catccgacct attccgccgt acaggcgcgg ctgctcgaag ccaaccgcct
     3541 ttcccccgaa ctgctcgcca aaagcctgtg catcatcggc gcgcaccacg tcgattacgc
     3601 cgacatctac tgccagcgca ccgcttatga aagctggcat ttggaagagg gcatcgtcaa
     3661 atcgggcagc ttccaaatca atcagggcgt gggcgtgcgc gccgtttcgg gcgacaaaac
     3721 cgcctttgcc tacgccgaca gcctgtgcat cgattcgata aaccgttccg cccgcgccgt
     3781 ccgcgcgatt ggggcggcag gcggcaaggt gtccgccaaa atgccgtctg aaacgcgcgg
     3841 caagccggtt tgttccgcgt ccgaccccat tgccggcctc gattccgccg ccaaagtcgc
     3901 gctgttgaac aaagtggaag caatcgccaa agccgccgat ccgcgcatcg tgcaagtgat
     3961 ggccggtttg acctgcgaat acgatatggt ttacctcgcc cgtctggacg gcaaacacgc
     4021 cgccgacatc cgcccgatgg tgcgcctgaa cgttaccgtt atcgccaaac agggcgaacg
     4081 ccgcgaacag ggcagcgcgg gcggcggcgg acgctacgac ttggcttatt tcgatgaaaa
     4141 cttggttcat cggtttgtcg atgccgccgt caaacaggca ctcaccaacc tcgaatcccg
     4201 ccccgcgccc gccggcgaaa tgaccgtcgt tttgggcaac ggctggccgg gcgtgttgct
     4261 gcacgaagcg gtcggacacg gtttggaagg cgatttcaac cgcaagggga ccagcgtctt
     4321 ttccggcaga ataggcgagc gcgtcgccgc caaaggcgtt accgtcgtcg atcagggcga
     4381 tattgccggc cggcgcggtt cgctcaacat cgacgacgaa ggcaacgaaa cccgccgcac
     4441 cgtattgatt gaagacggta ttttgaccgg ctatatgcag gacgaaacca acgcccgcct
     4501 gacgggtacg caatcgaccg gcaacggccg ccgcgaaagc tacgcctccg cccctatgcc
     4561 gcgcatgacc aataccttta tggaaaacgg cagctatgag ccggaagaaa tcatcgcgtc
     4621 catcgacaaa ggcatttacg ccgtcaactt cggcggcgga caggtggaca ttaccagcgg
     4681 caaatttgtg ttcagcgcgt ccgaagcatg gtgggtggaa ggcggcaggc tgcaatatcc
     4741 cgtcaaaggc gcgaccatta tcggcaacgg tcccgaagtg ctgaaacacg tttcgatgat
     4801 aggcaacgat accgcgctcg acagcggtgt cggcgtgtgc ggcaaagaag ggcagagcgt
     4861 ccccgtcggc gtggggcaac cgactttgcg gattgatgcc ggactgaccg tcggcggcag
     4921 cgcaatctga cgcggaatcc ggcacgatgc cgtctgaaag gttttcaggc ggccttttac
     4981 attaggataa aaccatgcag gaacagaatc ggaaatcaag ttttcccata gtgatgttgc
     5041 tggtgtcggt tgccctgtgg atagcgtctt tatccaatgt tgcattttat ttgggcaatc
     5101 atggaagcat ggagggtttg accgttttga ttttggggtc gatatttgct tctttggata
     5161 tcaggtattg tgcggtctat gcgaattatg tttggttggc ggccattgtt ttgctggcgt
     5221 tgcggaagaa ggtcgtgcct gtccatgcgg cactttgggg cttggcgttg gtggctttca
     5281 gtgtgaaagc cgtatacgtc gatgaagcag ggaatacatc ggatattgtg cgctacggtg
     5341 caggatttta tttgtggtat gccgcatttg cggttgccac catcggtacg tttgccggaa
     5401 agaataagga aagaaaagcc gcatcggcgg cagacgggat aaaaaatgac gtttgacgaa
     5461 tggttgggct tgtcaaaact gcctaaaatt gaagcaagaa tgctgctgca atatgtttcg
     5521 gaatatacgc gcgtgcagtt gttgacgcgg ggcggggaag aaatgccgga cgaagtccga
     5581 cagcgggcgg acaggctggc gcaacgccgt ctgaacggcg agccggttgc ctatatttta
     5641 ggtgtgcgcg aattttatgg cagacgcttt acagtcaatc cgagcgtgct gattccgcgc
     5701 cccgaaaccg aacacttggt cgaagccgta ttggcgcgcc tgcccgaaaa cgggcgcgtg
     5761 tgggatttgg ggacgggcag cggcgcggtt gccgtaaccg tcgcactcga acgccccgat
     5821 gcgtttgtgc gcgcatccga catcagcacg cccgcccttg aaacggcgcg gaaaaatgcg
     5881 gcagatttgg gcgcgcgggt cgaatttgca cacggttcgt ggttcgacac cgatatgccg
     5941 tctgaaggga aatgggacat catcgtgtcc aacccgccct atatcgaaaa cggcgataaa
     6001 catttgttgc aaggcgattt gcggtttgag ccgcaaatcg cgctgaccga cttttcagac
     6061 ggcctaagct gcatccgcac cttggcgcaa ggcgcgcccg accgtttggc ggaaggcggt
     6121 tttttattgc tggaacacgg tttcgatcag ggcgcggcgg tgcgcggcgt gttggcggag
     6181 aatggttttt cgggagtgga aaccctgccg gacttggcgg gtttggacag ggttacgctg
     6241 gggaagtata tgaagcattt gaaataattg tttgcaaaat tggcgggttt tccattattg
     6301 ggttatgatg tgtttcgata tgtaaacatt tataggtttg gagcgataaa caatgaatgc
     6361 agtcgtagtt gccgtaatcg ttatgctggt gctgtcgctg tcgcgcgtgc acgtggtatt
     6421 gagcctgacg gtcggcgcgt ttgtcggcgg cgcggtggcg ggtatgccgc tgcaaaacat
     6481 tgccgatgcg gcgggacagg tcagtcaggc ggggattatc cccgtgttca acaaaggttt
     6541 ggaaggcggt gcgaagattg cgctttctta tgcgatgctc ggcgcgtttg caatggcgat
     6601 tacccattcc ggcctgccgc agcagcttgc cggcgcggtc gtccgcaagc tgaaccgggg
     6661 cggtatgccc gacagcgtgc gttcgggcga gggcgcggtc aaatggctgc tgctttccat
     6721 catccttgtg atgggcatta tgagtcagaa catcatacct atccacatcg cctttatccc
     6781 gatgattgtt ccgccgctgc ttttggtgtt caaccgcctg aaaatcgacc gccgtctgat
     6841 tgcgtgcgtc attactttcg ggctggttac gacctatatg ttcctgcctt acggcttcgg
     6901 cgcgattttt ttgaacgaaa tcctgttggg caacatccat tccgccgcgc cgcagcttga
     6961 tgtgaaaaac atcaacgtaa tggcggcaat ggcgattccc gcgttgggaa tgctgaccgg
     7021 actcctgctg gcgtttgtcc attaccgcaa accgcgcctg taccaaagca acaatgccga
     7081 tacagcgggc aacgccgatg cggcaaaccg tccgcagccg tccgcctacc gcagcctggc
     7141 cgccgccgcc gccattgccg tatgttttgc catccagttg atgtatgaag actcgctggt
     7201 gttgggcgcg atgctcggtt tcgccgtatt tatgatgttg ggggtcatca accgcgacaa
     7261 ggcaaacgac gtattcggcg aaggtatcaa gatgatggcg atggtcggct tcattatgat
     7321 tgccgcgcag ggttttgccg ccgtgatgaa tgcgaccggg catatccagc cgctggtgga
     7381 aagcagtatg gcgatattcg gcaacagcaa aggtatggcg gcgttggcga tgctggtggt
     7441 gggacttttg gtaacgatgg gcatcggttc gtccttttcc actttgccga ttattgccgc
     7501 gatttatgtg cctttgtgtg tcggtttggg tttttcgccg cttgccaccg tcgccattgt
     7561 cggcacggcg ggggcgttgg gcgatgccgg ttcgcctgcg tccgattcca cgctgggtcc
     7621 gacgatgggg ctgaacgccg acgggcagca cgaccacatc cgcgattccg ttatcccgac
     7681 cttcatccac tacaacatcc cgctgctgat tgccggctgg attgccgcga tggtgctgta
     7741 aatggacgcg gttcaagagt tggaacaccg catcacagag ctggaaatcc aatccgcgct
     7801 tcaggaggac gtaatcgccg gcctgaacgc gatggtggcg gaattgcggc agacgctgga
     7861 tttgcaacag gctcagttga ggctgctgta tcaaaaaatg caggacagga atcccgacgc
     7921 gcaagagccg tattccctgc gcgacgagat tccgccgcat tattgatgcg ccgccgtatc
     7981 cggatttcct taaaaaagct gtgtttgaat attccgccga tgcggctcta agatgtaact
     8041 ttaatcagtc ggacaaaatg ccttttatcc gatggaaccg ttttccaccc gaacggaaat
     8101 aagcggcttt ccccctgcaa acagacgaaa tcaaccgggt attcaaacac agtcaaaaaa
     8161 aaaagccatc cgaacccaaa ggggacagac ggccaaacac attacgggga aaatatctta
     8221 ctcaatgagt ctgcgaagca gatattgcta agacatccgt aattataagg taa
//
LOCUS       DAWFAN010000081         8245 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWFAN010000081 DAWFAN010000000
VERSION     DAWFAN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.081"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(207..1592)
                     /gene="pntB"
                     /locus_tag="WGO65_001296"
     CDS             complement(207..1592)
                     /gene="pntB"
                     /locus_tag="WGO65_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ0327587.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFSKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKGANSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1658..2041)
                     /locus_tag="WGO65_001297"
     CDS             complement(1658..2041)
                     /locus_tag="WGO65_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ0327588.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAAQSTINTLACLFILFRLAFIWCYIA
                     DKAAMRSLMWAGGFACTVGLFVAAA"
     gene            complement(2045..3586)
                     /locus_tag="WGO65_001298"
     CDS             complement(2045..3586)
                     /locus_tag="WGO65_001298"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ0327589.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGWAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQPPSKKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLN
                     MFKKG"
     gene            3876..4709
                     /gene="serB"
                     /locus_tag="WGO65_001299"
     CDS             3876..4709
                     /gene="serB"
                     /locus_tag="WGO65_001299"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ0327590.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLSDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            4773..5996
                     /locus_tag="WGO65_001300"
     CDS             4773..5996
                     /locus_tag="WGO65_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0327591.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVARASEGRRLGVLILCGMLAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSGSAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6157..7737
                     /gene="purH"
                     /locus_tag="WGO65_001301"
     CDS             6157..7737
                     /gene="purH"
                     /locus_tag="WGO65_001301"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ0327592.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIHPAAGSLAAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDICR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDANFDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT         1830 a         2262 c         2334 g         1819 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 tcactgcaaa ccttcataaa cggggtttgc agtgattttt tcaaatcaaa cagattgaaa
      181 acctgcgccg aattgttcag acggcattat tttttcagtt cggacagaat gtcatccacg
      241 gttttcttcg catctccgaa acacattacg ctgttttcgt tgaagaacag cgggttttgt
      301 acacctgcgt agccggtatt catcgagcgt ttgaagacga cgacttcttt tgccttccat
      361 acttccaaca cgggcatacc cgcaatcggg ctgttcgggt cggtttgggc ggcaggattg
      421 acggtatcgt tcgcaccgat gaccaagacc acatcggttt cggggaaatc gtcgttgatt
      481 tcgtccattt ccaacacgat gtcgtagggg actttggctt cggcaagcag tacgttcata
      541 tgaccgggca ggcggccggc gacggggtgg atgccgaagc gcacttctgt gccgttttta
      601 cgcaaaagct cggtgatttc ggcaacgggg tattgcgctt gtgcgactgc cataccgtag
      661 cccggggtaa tgatgacact gtttgcgcct ttcagcattt cggcaacatc ggcagctttg
      721 acttctcggt attcccctat ctcttggctg ccggaagatg atgtgccgct gtcgctgccg
      781 aaaccgccgg cgattaccga gacaaacgac cggttcatgg ctttgcacat aatgtaggac
      841 agaatcgcgc cgcttgagcc gaccaacgcg ccggtaacga tgagcaggtc gttggagagc
      901 ataaagcctg ccgctgcggc cgcccagccg gagtaggagt tgagcatgga cacgaccacg
      961 ggcatatctg cgccgccgat ggaggcaacc aagtgccagc cgaatgcgag ggcaatcagg
     1021 gtcataatca gcaggatgaa gccgctgccg tcaatgccga cgaatacgag cagcaacaca
     1081 aacgatacgc caagtgccag tgcgttgagc ttgtgtttgg cgggcagttg cagcgggctg
     1141 ctgctgattt tgccgttgag tttgctgaat gcgaccagcg agccggtaaa ggttaccgcg
     1201 ccgatgaaga tgcctaaata cacttcgacc agatggatgg tgtgcatatc gtgcgaaacg
     1261 ttgcccggcg cgatatagct gttgaagccg accaaaaccg ccgctaggcc gacgaagctg
     1321 tgcagcaggg caatcagttc gggcatttcg gtcatttcca cctttttggc tttgtagatg
     1381 ccgattgccg cgccgatgag catggcgatg atgatccagc ccagtccgtg ggtattgtcg
     1441 gaaaaaacag ttacaaaaag ggcgaccgcc ataccggcga taccggaata gcagccctgt
     1501 ttggcggttt cctgtttgga cagccccgcc agtgagaaga tgaataaaat tgcggcaacg
     1561 atatacgccg ctgttacgag tcctgaagac atggaaattc tccgattttc gatgatttgt
     1621 tttcaatgcc gtccgaactg ttcagacggt atctgtttca agcagccgcg acaaacagcc
     1681 cgacggtgca ggcaaatccg cctgcccaca tcagtgagcg catagcggct ttgtcggcga
     1741 tatagcacca gataaaggcg aggcggaaca ggatgaacag gcaggcaagc gtgttgatgg
     1801 tcgattgcgc cgcattgccg gttgcgtgtg ccgtcaaaac ggcggcggca aacggtgcaa
     1861 aggcttcaaa accgttttgc tgcgcggcgt gggcacgggc ggctgtgcct tgcgtgtgcg
     1921 ccagaaaatc gcgcggattg tggttgtctt taaaccggaa tccgcccgct tttttggcat
     1981 atgccgcaca aaaaagcggc aataggcagg caatcaggat gcaccaatag gcgaaagtca
     2041 tggcttaccc tttcttaaac atattcagca tacgccgtgt aaccgcaaag ccgccgaaga
     2101 tgttgatgcc ggcaatcagg atggcaacaa acgacagcag cgaaacaaag ccgttgccct
     2161 gaccgatttg cagcagcgcg ccgacgacga tgatgccgga gatggcgttg gtcacggaca
     2221 tcagcggcgt atgcagcgag tggctgacat tccaaacgac gtagtacccg atgacgcagg
     2281 cgaggacgaa cacgataaag tggttcaaga atgctgcggg tgcgaccgcg ccgacccaca
     2341 gcactaatac ggcggcgatg acggcaggcg cgagtttttt ccacagggga acgggtttcg
     2401 gctcgggctt ggcggcaggc gcggcttttt tagacggcgg ttgctgcggc tgggcggaaa
     2461 cttgaatcgg cggaggcggg aaggtgattt cgccgtcgcg ggtaacggtc atgttgcgga
     2521 taatcacgtc ttcaaaatcc agcgtgattt cgccgtcttt gttcgggctt aacagcttgg
     2581 tcaggttcac caagttggtg gcgtaaagct gggaagactg tccggcaagg cggtttgcca
     2641 tgtcggtgta gccgatgatt ttcacgccgt tgccggttac ggacaattcg cccgggcggg
     2701 tgagttcgca gttgccgccc gtcgccgccg ccaaatcgac gatgacggag ccggatttca
     2761 tgctttccac catttctttg gtaatcagct tgggcgcggg tttgcccgga atggcggcgg
     2821 tggtgatgat gatgtccact tctttcgcct gctcggcaaa gagcttcatc tcggctgcga
     2881 taaattcgtc gctcatcact ttggcgtagc cgtctccgct gccgcccgat tcttgtggga
     2941 agtcgagttt caggaacttg ccgcccatcg attcgatttg ttccgccact tccaagcggg
     3001 tatcgaacgc gcgcaccact gcgccgagcg agtttgccgt accgatcgcc gccaaacctg
     3061 ccacacctgc gccaatcacc aaaacctgcg cgggcggcac tttgccggcg gcggtgattt
     3121 ggccggtgaa gaaacggccg aaggcgttgg cggcttcaat tacggcgcgg tagccgctga
     3181 tgtttgccat cgaagacaaa gcgtccaaag cctgcgcgcg cgaaatgcgg ggcaccatgt
     3241 ccatcgccag cgcgttcact ttcttggcgc gcaaggcttc gaccaaagcc tcgttttggc
     3301 gcggccacag gaagctgacg atggtttgac cttcgttcaa aagcggcagc tcgccttcgg
     3361 acggcgcatt gaccttataa atcaaagggc aggcccaaac cgccgctttg tcggcaacgg
     3421 tcgcgcctgc tgtttggtaa gcggcatcgt ccaaacttgc cgcccaacct gcaccgcttt
     3481 cgacaacggt ttcaaagccc agtttgccca gcagggcgac ggtggcgggc gtacaggcga
     3541 cgcgggtttc gccggataat gactcgcgtg ggataccgat tttcatctct gaatcctttt
     3601 tcgggttgtt tatatgtatc gtgggttaaa tttaaatcgg ggcggggcgg agcaacgccg
     3661 taccggttta aaaccgattc acttcaaatg ttaatatatt ttagataatc cccttataac
     3721 gaattttcat caggctggca atagttgcgg cattttcccg tgttgtccga cacatactgc
     3781 cgtttgcgtt tgaagtcatt ttccgattgc agtcccgccc gccgtaacat tctttgccgt
     3841 ttcactatat aatccgcatt ttttgagccg ccgttatgcc gcacgccctc gtcctccaat
     3901 ttccctccgc cgaagccctg ccttccgact tcccctcccg cctgcccgaa cctgattgcg
     3961 ctgatgaaaa gcgtatgcgt tttattgttg aagaaggatt ttctttaagc gaaaaagacg
     4021 cggcgttact tggcagccgt caaatcgacc acgccgtgtt gtcggatatg gattttggcg
     4081 aactcggttt gattgtcagc gatatggatt cgacgctgat taccatcgaa tgcgtcgatg
     4141 aaattgcggc aggcgtgggt ttaaaagaca aggtagcgga aattaccgag cgttcgatgc
     4201 gcggagagtt ggatttcgag cagtcgttgc gcagccgcgt tgcgctgttg gcgggattgg
     4261 acgaacagat tttggcggac gtttatgaaa acgttttgaa gctctcgccc ggtgcggaat
     4321 ttttgttgga cgaatgcaaa aggcacgatg tgaaattcct gctggtgtcc ggcggcttca
     4381 cgttttttac cgaacgtctg caacaacgcc tcggcttcga ataccaacac gccaatgttt
     4441 tggaaattga aaacggcagg ttgaccggcc gtctgaaagg cagaatcatc gacgcgcagg
     4501 caaaggcgga tttattgcgc gaataccgca gccgcctcgg attgcagcca catcaggtgt
     4561 tggcgatggg cgacggtgcg aacgatattc cgatgctcaa agaagcgggc ataggcgtgg
     4621 cttaccgtgc caaaccgaaa gcgcgggccg ccgccgatgc ctgtatcaat ttcggcggtt
     4681 tggagcgtgt acgcggcctg ttcggatagg cggataggaa acggatgtcg tccgaaaggt
     4741 tttcagacgg catttgaacg gcaggaacga cagtgggacg cagaaaactt tggtttgccc
     4801 tggcagcagc aggcgttatc ggcggtttgg tcggcattgt gctgacggaa ctgatgcact
     4861 tcatacagca tacggcatac ggttatggcg cggacggtgc gtacatctcg ttccgcgaag
     4921 gtgtggcccg tgcttcggaa gggcggcgtt taggcgtatt gattctgtgc ggtatgttgg
     4981 caggcggcgg ctggtggttg ctcaaacgtt tcggtaagcc gcaaatcgaa atcaaagccg
     5041 ccttgaaaca gccgttgcag gggctgccgt ttctgacgac ggttttccat gttctgctgc
     5101 aaatcataac ggtcggactc ggttcaccgc tcggacggga agtcgccccg cgtgaaatga
     5161 ctgccgcgtt cgcttttgcc ggcggcaggc ggttgggttt ggatgaagac gaaatgcggc
     5221 tgctgattgc ttgcgcttcg ggtgcgggtt tggcggccgt gtataacgtg ccgctcgcct
     5281 ccacactttt tatcctcgaa gccatgctgg gcgtgtggac gcagcaagcc gtcgccgccg
     5341 cattgctgac ttccgtcatc gccaccgccg tcgcgcgcat cggcttaggc gacgtgcagc
     5401 aatatcatcc cgccaacctt gccgtaaaca cttcattact ttggttttcc gccgtcatcg
     5461 gcccgatact gggcgcaacc gccgtctttt tccagcgcac cgcccaaaaa ttccccttca
     5521 tcaagcgcga caacatcaaa atcattccct tggcagtctg catgtttgcg ctcatcggcg
     5581 tgattgccgt ttggtttccc gaaattttgg gcaacggcaa agcaggcaac caactgacct
     5641 ttggcggttt gaccggttgg caacacagcc ttgagctgac cgccgtcaaa tggctggttg
     5701 tcttgatggc gttggcggca ggcgcatacg gcggactgat tacgccgtct atgatgctcg
     5761 gcagcaccat ttcttttgca gccgccgccg cgtggaacag cgttttcccc gaaatgccgt
     5821 ctggaagcgc ggcagtcgtc ggagcggcgg tgtttctcgg cgtatcgctc aaaatgccgc
     5881 tgaccgccat tgtgttcgta ttggaactga cctacgcgcc gctgtccctg ctgctgcctt
     5941 tgtgcatcgg catggcgggg gcattgggaa catcgggaaa aatgggcttc aaataaatgc
     6001 cgacaaatgc cgtctgaaag gttttcagac ggcatttctt tgtaaattgt cagcaatatc
     6061 tgcaaaaacc tactgccaaa aaactagaaa tagcggataa taccgccccg aaaaagttcc
     6121 cctttcccca tccaactgtc agaaaaggaa gccccaatgt ccgtcatcaa acgcgccctg
     6181 atcagcctgt ccgacaaggc aggcgcggtc gaatttgccc aaactctgca caagctcggt
     6241 gtcgaaatcc tctccaccgg cggcacggcg aaactgcttg ccgatgcggg cgttcccgtg
     6301 attgaagtcg ccgactatac cggcttcccc gaaatgctcg acggccgcgt gaaaacgctg
     6361 catccgaaaa tccacggcgg tattttgggt cggcgcgatt tggacgaaca cgttgccaag
     6421 atggaagaac acggtatcgg caacatcgac cttgtgtgtg tcaacctcta ccccttcgcc
     6481 gccaccatcg ccaaaccaaa ctgcacactg gaagacgcga ttgaaaacat cgacatcggc
     6541 ggcccgacta tggtgcgttc cgccgcgaaa aactggaaac acgtcgccat cgttaccgac
     6601 accgccgact tccccgccat tgccgccgaa ctcgaagcca acaacggcgc attgagcgac
     6661 aaaacccgtt tcaacctctc acgcaaagca ttcagccaca ccgcccaata cgacggcatg
     6721 atttccaact acctgacctc cctttcagac gacgttttga gcggccagcc ccaaatcggc
     6781 gaattcccaa gccagttcaa ccaaagctgg attaaagtac aagacatgcg ctacggcgaa
     6841 aatccgcacc agcgcgccgc gttctaccgc gatattcacc ccgccgccgg cagcctcgcc
     6901 gcctacaaac agctgcaagg taaagaattg tcttacaaca acatcgccga tgccgatgcc
     6961 gcttgggaag ccgtcaaatc cttcgacgcg cccgcctgcg tgattgtgaa acacgccaat
     7021 ccgtgcggcg ttgccgttgc agccgatacc ttgaccgcct acaaactcgc ctacgccacc
     7081 gacaccacca gcgcgttcgg cggcatcatc gccttcaacc gcgaagtgga cggcgaaacc
     7141 gtcaaacaga ttaccgacaa ccagtttatg gaagtcttga tggcgccgaa gtttaccgcc
     7201 gaagccctcg aaatcgccgc cgccaagaaa aacgtgcgcg tattggaagt gccgcttgag
     7261 gcaggcgcaa accgcttcga actcaaacgc gtcggcggcg gactgttggt acaaacgccc
     7321 gacatctgcc gcatcagccg cgccgatttg aaagtcgtct ccaaacgcca accgaccgag
     7381 caggaatgga acgatttgct gttcgtctgg aacgtcgcaa aatacgtcaa atccaacgcc
     7441 atcgtgttcg gcaaaggcgg acaaacctac ggcatcggtg caggccaaat gagccgcgtg
     7501 gacagcaccc gcatcgccgc ccgcaaagcg caggacgcca atttcgacct caacggcgcg
     7561 tgtgccgcct ccgatgcctt cttccccttc cgcgacggcg tggatgtgat tgccgaacag
     7621 ggcattaaag ccatcatcca tccggcaggt tcgatgcgcg atcaagaagt cttcgacgcg
     7681 gcagacgaac acggcattgc gatggtggta accggcatcc gccacttccg ccattaaggc
     7741 agatgaacaa ggcaatgccg tctgaagggc tttcagacgg cattttgcgc tattttgtga
     7801 aggtagggat gacggttcgg gtattcctga cagggtggat tttcaaggtg ttgtataggg
     7861 tgtaggagga ttcgtaaaag gtgggatgca gggtgtgctt cagcccgctg catcaaaaat
     7921 ttttggagaa ccggcgggag tcggcggttt tggtttcggc ggggacggtg gaaatgggta
     7981 acattgacgg aatcgacgga agcggtgggc tgaagcccac ccttgtatat tggaatcact
     8041 gtatcatagc aacaaaccgc ccggccgcca cccgcgccca cccaaggcac acaaccgttg
     8101 cgtagctcag ggagcggcag ggcaacccat cgacacaacc ggacagttgc cggacaacac
     8161 aaccgaatgc aaggcaggtt tatgatgagt acccaatacc attacgcagg tatagtgaat
     8221 taaatctaaa ccagtacagc gttgg
//
LOCUS       DAWFAN010000082         8196 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWFAN010000082 DAWFAN010000000
VERSION     DAWFAN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.082"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(95..1027)
                     /gene="cysK"
                     /locus_tag="WGO65_001302"
     CDS             complement(95..1027)
                     /gene="cysK"
                     /locus_tag="WGO65_001302"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ0327593.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVPNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1221..1826)
                     /locus_tag="WGO65_001303"
     CDS             complement(1221..1826)
                     /locus_tag="WGO65_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327594.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1823..2674)
                     /gene="dapF"
                     /locus_tag="WGO65_001304"
     CDS             complement(1823..2674)
                     /gene="dapF"
                     /locus_tag="WGO65_001304"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ0327595.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAEWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVEQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            2811..3107
                     /locus_tag="WGO65_001305"
     CDS             2811..3107
                     /locus_tag="WGO65_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327596.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPTVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3121..3789
                     /locus_tag="WGO65_001306"
     CDS             3121..3789
                     /locus_tag="WGO65_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0327597.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3862..5982)
                     /gene="pnp"
                     /locus_tag="WGO65_001307"
     CDS             complement(3862..5982)
                     /gene="pnp"
                     /locus_tag="WGO65_001307"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ0327598.1"
                     /translation="MFNKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6219..7082)
                     /locus_tag="WGO65_001308"
     CDS             complement(6219..7082)
                     /locus_tag="WGO65_001308"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ0327599.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAEEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7281..8144
                     /gene="rfbD"
                     /locus_tag="WGO65_001309"
     CDS             7281..8144
                     /gene="rfbD"
                     /locus_tag="WGO65_001309"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ0327600.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1954 a         2368 c         1902 g         1972 t
ORIGIN      
        1 gttttcggca tatatgcaaa aacgcatttt ccggcacggc gttacaccga taccgaaaaa
       61 tgcgtctgaa tgtttcgaca atccgattaa agcattatgc caaatctgca aaaagtggcg
      121 tagagagata gcgttcgcca taagaaggca gcagcacgac tatcagcttg ccttcgtttt
      181 caggctgttt ggcaagctgc aacgcactcc aaaccgccgc accggaagaa atacccacca
      241 aaatgccttc tttttccgct attgcgcggg cggtttcaaa agccgcttcg ttcggcactt
      301 tggtaatgct gtcgtaaatt ttggtattca aaacggtcgg aataaagcct gcgccgatgc
      361 cttgaatcgg gtgcgggcct ttttcgccgc cgctcaatac gggtgaagct tcaggctcga
      421 cggcaaccac tttaacttcg ggtttgtatt ttttcaacac ttcgcccacg ccggtaatcg
      481 taccgcccgt gccgacgccg gcaacgaaga catcgacttt gccgtccgta tcccgccaaa
      541 tttcctcggc ggttgttttg cggtggactt cggggtttgc ctcattgtcg aactggcgcg
      601 gcataaaata agtgtcggga tgcgcgtcca ccaaggattt cgctttggca atcgcgcccg
      661 ccataccttc ggcggcaggg gtcagaatca gctccgcacc aaacgcgcgc aacagcattt
      721 tgcgctcctt actcatgctt tccggcatgg taatcgccag cttgtagccg cgtgcggcac
      781 ataccattgc caaaccgaca cccgtattgc cgctggttgc ttcgacaatg acggtgtttt
      841 tgttgatttt gcccgctttt tcggcaccct cgatcattgc ttcggcaatg cggtctttga
      901 cgctgctgcc tggattgaaa aattccagtt tcacggcaac ctccgccttc aaaccttcgg
      961 tcagacggtt cagtttgacc aaaggcgtgt tgccgattag ttcggtgatg ctgtttgcaa
     1021 ttttcatctt ctgctcctga ataataaggt ttcgacagta aggcgcaatc cccataggca
     1081 tcctgtccga ttgcagggga tttgcgccga aacataaaca aatttcggat atggctttat
     1141 cctaaaccta tgccgcctaa aaaaccaata tccaaaattt ctttctttat aactttttgt
     1201 tccgatacgg ccgcaccgta ttatcttcca atttctgaaa gcagcagcac cagtaccgag
     1261 acctgcatca acgctgccgt caacccgccg agtcgcagct gcgagccgcc gaacgttgac
     1321 ggcggctgcc ggtctatacc gcagcggtgg acataggcaa ccgccatcag cacaaactgc
     1381 aacacgaacc aataaaccgg cttcagcgca taaagtttga gcagtgccgc aaacacaaac
     1441 acttgaaccg ttcccgaaaa gcaataatgc accaacgcac atattccgac aaaagcaagc
     1501 gtcatcgata cattactgac ggcaaaaagc ccgagcagcg gatgttcggg gtctgcctgc
     1561 catccgtttc catctgtttt ccggttccaa tgcccgatga aaaaaaatat gctgcccaaa
     1621 gtcaggtaaa aatgggggat gaacaagggc gcggcgcggg tcattcccca tatgccgggc
     1681 atcagctttg cccccaaaac cgatatgccc agccacagca taatactcgc ccaaaaccat
     1741 tgcaacattc ctgcgcggaa tatcagaaac agaaaaaccg ccgcatacag taaggcagac
     1801 aaaaaatcca aatgcagcaa aatcatgaat acgccaactc gccttcaaac accgtttccg
     1861 cagggccggt catcatcaca tcgccgccgc aggcccattc gatatataaa gttccgcccg
     1921 gcaaaatcac ctctaccgtt ttcccttcat ccaacaaccc cagacggata cccgccacca
     1981 cagccgcaca cgcgcccgta ccgcacgctt gggtttcgcc cacgccgcgc tcgaacacgc
     2041 gcaaacgaat cgcggttcgg ctgacaatct gcataaagcc gacattgacg cgttcgggaa
     2101 actgcctgtg cggttcgata agcgaaccgg tttcgcgcac cggcgcgcat tccacgtcat
     2161 cgaccacaat caccgcatgg ggattgccca tattgacgca gctgacaggc tgaatgccgg
     2221 attcgagatg caccccgtaa atacaggcat catccccctc gcccgattcg gggacaaacg
     2281 gtatttcaga cggcataaac ctcggtttgc ccatattgac cgtaaccatg ccgttatcgg
     2341 acaatttcgg aaaaataacg ccatttgccg tttcaacaca aatttctttc ttatcggtca
     2401 aacccttgtc tgcaacaaaa cgggcaaaac aacgcgctcc gttgccgcat tgctcgacct
     2461 cgctaccgtc ggcattgaaa atacggtaac ggaaatccac accttcggtt tccgaacgcc
     2521 cgaccaccaa aagctggtcg aagcccacgc cccggaagcg gtccgcccat tccgcaatcg
     2581 gcgcgtcctc gggggtaaaa tcctgactga ccgcgtcaat caccataaaa tcgttgccca
     2641 aaccgtgcat tttggtaaat ttcaaagtgt tcatattgtt ccgaaatccc gtatcgtctg
     2701 ttgcttccga atccgaaatc ccccgcaccg aagggaaatg cttccatctc ccgccgcctg
     2761 aggattaaaa tcagccatta tactgtttgt taccccttac aagaaacacc atgttccgca
     2821 tattactgtt gcttttccct tgtgcaatcc ttgccgcctg cacccgaaac ataccggcaa
     2881 acccgaccgt ttcaaattgc gcgcatcttc aaaaccgcac catcctgtat tttgacgcac
     2941 acggcaaacc cgccgacggc gcagtaaagg gcggatttta caaaattatc gaaatacagg
     3001 gtgcaaactg catccttatt caagacttta acagcacggg caatgccctc ggcggatcct
     3061 atgtgccgga ctgccgcaaa tcatcccttt tcccgaaaaa tatttgactt caaactgcct
     3121 atgaaccacg acatcacttt cctcaccctg ttcctactcg gtttcttcgg cggaacgcac
     3181 tgcatcggta tgtgcggcgg attaagcagc gcgtttgcgc tccaactccc cccgcatatc
     3241 aaccgctttt ggctgatcct gctgcttaac acaggacggg taagcagcta tacggcaatc
     3301 ggcctgatac tcggattaat cggacaggtc ggcgtttcac tcgaccaaac ccgcgtcctg
     3361 cagaatattt tatacacggc cgccaacctc ctgctgctct ttttaggctt atacttgagc
     3421 ggtatttctt ccttggcggc aaaaatcgag aaaatcggca aaccgatatg gcggaacctg
     3481 aacccgatac tcaaccggct gttacccata aaatccatac ccgcctgcct tgcggtcgga
     3541 atattatggg gctggctgcc gtgcggactg gtttacagcg cgtcgcttta cgcgctggga
     3601 agcggtagtg cggcaacggg cgggttatat atgcttgcct ttgcactggg tacgctgccc
     3661 aatcttttgg caatcggcat tttttccctg caactgaaaa aaatcatgca aaaccgatat
     3721 atccgcctgt gtacgggatt atccgtatca ttatgggcat tatggaaact tgccgtcctg
     3781 tggctgtaag ccccattcat tcccgatacc cgtaaaaaat accgtctgaa acctgttcag
     3841 acggcacttt caccctaagc gttactcggc ggcattttcc tcacgggcag gcgcgtccag
     3901 cagggctttg atggacagac ggacacggcc tctgtcgtcc acttccaatg ctttcacgtt
     3961 caccacctga ccgacttgca ggtagtcgcc gacattgcgt acgcgctcgt gggcgatttg
     4021 gctgatgtgt accaaaccgt ctttgcccgg catcacactg acaatcgcgc cgacattgtt
     4081 gtcgaggatt ttcaccacag tgccttcgta cactttgccc acttccactt cggcagtaat
     4141 ctgctcgatg cgttttttcg ccgcatcgcc ggcttcttga gtggttgcgg caatggtaat
     4201 cgtaccgtct tcggcaatat tgatttccgt accggtttca gcggtaatcg cacggatggt
     4261 ttcaccgccc ttaccgataa cttcgcggat tttgtcttgg ttgattttca tcgtgaacaa
     4321 gcgtggcgcg tgtgcggaca gctcttgcgg gcccgcaacg gcggctttca tctgatccaa
     4381 gatgtgcaga cgcgcttctt tggcctgcgc caaagcgatt tgcataatct ctttggtaat
     4441 gccttggatt ttgatgtcca tttgcagcgc ggtaacgcct tcggtcgtac cagccacttt
     4501 aaagtccata tcgcccaagt ggtcttcgtc gcccaaaatg tcggtcagga cggcaaactt
     4561 attgccttcc agaatcagac ccatcgcgat accggcaacg tgtgctttca aaggcacgcc
     4621 ggcggacagc aggctcaagc agccgccgca gacggaagcc atagaggaag agccgttgga
     4681 ttcggtaatt tcggagacca cgcgcatggt gtagctgaaa tcttcaggtt tcggcaatac
     4741 ggccaacaat gcacgtttag ccaaacgacc gtgaccgatt tcacggcgtt tcggcgcgcc
     4801 catgcggccc acttcgccgg tagagtacgg cggaaagttg tagtgcagca taaagcggtc
     4861 ggtgtattcg ccggacagcg cgtcgatgat ttgctcgtcg cgcgaagtac ccaaagtcgc
     4921 tacggcaagg gcttgggttt cgccacgggt aaacaatgcg gaaccgtgcg tgcgcggcaa
     4981 tacgccggtt tggatgttca gcggacggac ggtgcgggtg tcgcggccgt cgatgcgcgg
     5041 ttggccgtcc aaaatttggc tgcggacgac atcggcttcc aagtgtttga aaatgccttt
     5101 gatttcgttg gctgccagcg tgtcggtttc ttcggtaatc aaggcttctt ttaccgcact
     5161 ccaagcttcg tccaatttgg cagaacgcgc ttgtttttga cggattttga acgcttcttt
     5221 aatggtttcg ccggcaatcc cgcggacttt ggcaaccagt tcctcattgg tttcaggtgc
     5281 tttccaatcc caaagttccg gattgacttc gtcggcaaat tcattgattg cattgatggc
     5341 aacctgcatt tgatcgtggc cgtaaaccac cgcgcccagc atcacgtctt cgggcaggat
     5401 tttggcttcg gattccacca tcaacacggc ttttgaagta ccggcgacca ccaagtccaa
     5461 ttgcgatttc gccaattcgg ctttagtcgg gttcaaaacg tacacgccgt ttatataacc
     5521 cacgcgtgcc gcaccaatcg gaccggcaaa cggtacgccg ctcaacacca gcgcggcaga
     5581 cgcgcccaac attgcaggaa tgtcggaatc gatttcggga tcgacggaca cgaccatcgc
     5641 tacgatttgg atgtcgtggt agaaaccttc agggaacagc ggacgaatcg gacggtcgat
     5701 cagacggctg gtcaggattt ctttttcgct ttgtttgcct tcgcgtttga agaaaccgcc
     5761 gggaattttg cctgcggcgt aagtgcgctc gaggtaatcg acagtcagag ggaagaagtc
     5821 ttggccttct ttcacttctt tgttggtggt aacggcaacc aaaaccacgg tgtcgcccat
     5881 agagacttta acggcagcgg cggcttggcg ggcgatttcg ccggtttcca aagtaacggt
     5941 ttgattaccg tattggaagg tcttaacgtg tttattgaac attattgttc ctttcaaaat
     6001 accgcactgc taaaacacta ataatgcaca ctaaaatccg aatgtgcata gttagggttt
     6061 cagaccgtgc ggcaggttgt aaacaagctt tcagacgaca tttcggacgc tgaaagcaat
     6121 tccagattat aaaggcaacc atccttaaaa tccagtattg atacaataaa aaggccgtct
     6181 gaaccatatt tctttcagac ggccttcaaa cttaaaaatc aatcctgagt cagcaaagtc
     6241 aatgcttccc gatacttctc gacagttttc tgaatcacat cggcaggcac tttcggcgca
     6301 ggggcttttt tgttccaacc gctttgttcc agccagtcgc ggacgaattg tttgtcaaaa
     6361 gacggcggat tggtgccgac tttgtattgg tcggcaggcc aaaaacggct cgaatcggga
     6421 gtcaatacct catccatcag cgtcagcgta ccgtttgtat ccaaaccgaa ttcaaatttg
     6481 gtatcgcaaa tgatgatgcc gcgcgatttg gcatattccg ccgcttctgt ataaagctga
     6541 atcgctttgg cgcgcacttc ttccgccaat tctttgccga taatgcgttc gcattcttca
     6601 aagctgatgt tttcatcgtg atcgccgact gcggctttgg ttgagggcgt aaaaatcact
     6661 tcaggcagtt gttgcgcttc ctgcatacct tcaggcagtt gaataccgca aaccgagccg
     6721 gttttttgat aatctttcca accgctgcct gccagataac cacgcacaat cgcctctact
     6781 ttcaccggag tgagcttttt agccacgacg gcgcgtttct ctaaagcttt ggcttcgttt
     6841 tcaggcaaaa catcgtaaac cgtttgaccg gtaaagtggt tgggcataat atgcgccagt
     6901 tttttaaacc aaaaattgga aatctgcgtc agaatctccc ctttgctcgg aatcgggtcg
     6961 tccaaaatca catcaaacgc ggacaggcgg tcggaagcga ccatcagcat acgtttatcg
     7021 tcgatttcat ataaatcgcg cacttttcca gaatagatct ttaccaaacc aatctcactc
     7081 atttcgcccc ccctgaaaat atcttgaaaa taccgacccg acacccgaca ggtttgaatc
     7141 acaaaccgat attctaaccg aagtcggcgc aaaacaatac ccatggcaca aaaagccaac
     7201 ccgtcaaccg tcggcaaagt tttggcacta taataccgac agcaagtcct acaatacact
     7261 ttcaccaaag gaaatacctc atgagaatcc tattgacagg ctcgaaaagc caactggcac
     7321 gctgcctgcg cgaccgtctt ccggaagact gggaaaccat tgcgacggat tccgcatccc
     7381 tagacattac cgatgccgat gccgtctgca acatggtcaa aagtttccaa cccgacgcca
     7441 ttgtcaacac ggctgcctat actgccgtcg acaaagcgga aggcgatgcg gcagcggcat
     7501 ttgccgtcaa tgcttccgcc gtttacaacc ttgccttggc agcacatcgc gcccatgccc
     7561 gattcatcca catctcaacc gactatatct ttgacggtaa agggaaaagg ccctatcagg
     7621 aaagcgactt taccaatcct tccaatgtat acggacaatc caaaaccgcc ggcgaactgc
     7681 tcgcactgtc tgccaatccc gacagcctta tcctgcggac ttcttggctg tttagcgaat
     7741 acggggacaa ctttgtccgc accatgctga accttgcgcg ggaacgttcc ccgctgtccg
     7801 ccgtccacaa ccaaatcggc tgcccgacct atgccggcga cttgtccgcc gccatcatcc
     7861 gcctgttgca gcagtccaat cccgttcgcg gcatttacca ctacgtcggc ggcaaatccg
     7921 tatcctggta cgaatttgcc caacatattt tccaagcggc atcgcaacag cagacatcct
     7981 tccccgttcc cgaattgact gccgtttcag acaaggaata tccgaccgcc gcccccaggc
     8041 ccgcatacag cattttggac tgccgcaaaa tcgaaaacga cttcagcatc aaaccgtcag
     8101 actggcaaaa agcccttgca caggtcgttt ccaagctgct ctgatgccgc ccgcctctcc
     8161 gtttccgccg tcaagcaccg ccttggcggt tttctt
//
LOCUS       DAWFAN010000083         8192 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWFAN010000083 DAWFAN010000000
VERSION     DAWFAN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.083"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            110..1279
                     /gene="prpF"
                     /locus_tag="WGO65_001310"
     CDS             110..1279
                     /gene="prpF"
                     /locus_tag="WGO65_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ0327601.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1692..2891
                     /locus_tag="WGO65_001311"
     CDS             1692..2891
                     /locus_tag="WGO65_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0327602.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIEAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLSNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
     gene            3249..3869
                     /locus_tag="WGO65_001312"
     CDS             3249..3869
                     /locus_tag="WGO65_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ0327603.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(3996..4439)
                     /locus_tag="WGO65_001313"
     CDS             complement(3996..4439)
                     /locus_tag="WGO65_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ0327604.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4613..4981
                     /locus_tag="WGO65_001314"
     CDS             4613..4981
                     /locus_tag="WGO65_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0327605.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            5026..6180
                     /locus_tag="WGO65_001315"
     CDS             5026..6180
                     /locus_tag="WGO65_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0327606.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVLAWLSAVFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6237..7109)
                     /locus_tag="WGO65_001316"
     CDS             complement(6237..7109)
                     /locus_tag="WGO65_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ0327607.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            7194..8006
                     /locus_tag="WGO65_001317"
     CDS             7194..8006
                     /locus_tag="WGO65_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ0327608.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTISAGREVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1781 a         2244 c         2370 g         1797 t
ORIGIN      
        1 ggggcgaacc ccgacagttt gcggaatcaa acggctttgg tcggagtggc agcctaatcc
       61 actataaaaa tcgtgggcag agcccacgct acataaggag aatctagaaa tgccgcaaat
      121 taaaattccc gccgtttact accgtggcgg tacatcaaaa ggcgtgtttt tcaaacgttc
      181 cgacctgccc gaggcggcgc gggaagcggg aagcgcacgc gacaaaatcc tcttgcgcgt
      241 actcggcagc ccggatccct acggcaagca gatagacggt ttgggcaacg ccagctcgtc
      301 caccagcaag gcggtgattt tggacaagtc cgaacgcgcc gatcacgatg tcgattacct
      361 tttcgggcaa gtttccatcg acaaaccttt tgtcgattgg agtggcaact gcggcaacct
      421 caccgccgcc gtgggcgcat ttgccatcga gcaaggcttg gtcgataaag gcaagattcc
      481 ttcagacggc atctgcacgg tcaaaatctg gcagaaaaac atcggcaaaa ccattattgc
      541 ccatgtaccg atgcaaaacg gcgcagtttt ggaaacaggc gattttgagc tcgacggcgt
      601 aacgttcccg gcagccgaag tacaaatcga atttcttgat ccagccgacg gtgaaggcag
      661 tatgttccca accggcaatt tggtcgatga aattgatgtg ccgaatatag gccgtttgaa
      721 agccacgctc atcaacgcgg gcattccgac cgttttcctg aatgccgccg acttgggcta
      781 cacgggcaaa gagttgcaag acgacatcaa caacgatgcc gcggctttgg aaaaattcga
      841 gaaaatccgc gcttacggtg cgctgaaaat gggtctgatc agcgacgtat ccgaagctgc
      901 cgctcgcgcg cacacgccga aagtcgcctt cgtcgcgccc gccgccgatt acaccgcctc
      961 cagtggcaaa accgtgaatg ccgccgacat cgatttgctg gtacgcgccc tgagcatggg
     1021 caaattgcac cacgcgatga tgggtaccgc ctctgttgcc attgcgaccg ccgccgccgt
     1081 gcccggtacg ctggtcaacc ttgccgcagg cggcggaacg cgtaaagaag tgcgcttcgg
     1141 gcatccttcc ggcacattgc gcgtcggtgc agccgccgaa tgtcaggacg gacaatggac
     1201 ggccaccaaa gcggttatga gccgcagcgc acgcgtgatg atggaaggtt gggtcagggt
     1261 gccggaagat tgtttttaaa ttgacgtagc atgggtttgc ccgcgagcca taaacaggtc
     1321 gtctgaaaaa caagtaaaca tcaaatcact gaccattcct ttcccttgcc ctgtggcgga
     1381 aggcggcaaa tcacaaggaa gaacacggaa accccgataa aagacagctt cccgtattac
     1441 cgtcattccc gcgcaggcgg gaatctagac ctgtcaatat ggaggattgg taggggaaaa
     1501 caggtttcgt gagttctaca ttctggattc ccgccacagc ctgtcctcgc gtaggcgggg
     1561 acggaataac gatagaaaat gcggcatacg ctttgcccaa agaggccgtc tgaaacacct
     1621 tgcgcctgat gtctgccttt ttcagacgac cccacaccaa aaaacaacca caaactacaa
     1681 ggagaaacat catgtccgac caactcattc ttgttctgaa ctgcggcagt tcatcgctca
     1741 aaggtgccgt tatcgaccgc aaaagcggca gcgtcgtcct aagctgcctc ggcgaacgcc
     1801 tgaccacgcc cgaagccgtc attacgttca acaaagacgg caacaaacgc caagttcccc
     1861 tgagcggccg aaactgccac gccggcgcgg tgggtatgtt attgaacgaa ctggaaaaac
     1921 acggactgca cgaccgcatc gaagccatcg gccaccgcat cgcccacggc ggagaaaaat
     1981 acagcgagtc tgttttgatc gaccaagccg tcatggacga actcaatgcc tgcattccgc
     2041 ttgcaccgct gcacaacccc gccaacatca gcggcatcct cgccgcgcag gaacatttcc
     2101 ccggcctgtc caatgtcggc gtgatggata cttcgttcca ccaaactatg cccgaacgtg
     2161 cctacactta tgccgtgccg cgcgagttgc gtaaaaaata cgccttccgc cgctacggtt
     2221 tccacggtac cagtatgcgt tacgtcgccc ccgaagccgc gcggattcta ggcaaacctc
     2281 tggaagacat ccgcatgatt attgcccacc tcggcaacgg cgcatccatt accgccatca
     2341 aaaacggcaa atccgtcgat accagtatgg gtttcacgcc gatcgaaggt ttggtaatgg
     2401 gtacacgttg cggcgacatc gatccgggcg tatacagcta tctgacttcc cacgccggga
     2461 tggatgttgc ccaagtggat gaaatgctga acaaaaaatc aggtttgctc ggtatttccg
     2521 aactttccaa cgactgccgc accctcgaaa tcgccgccga cgaaggccac gaaggcgcgc
     2581 gcctcgccct cgaagtcatg acctaccgcc tcgccaaata catcgcttcg atggctgtgg
     2641 cctgcggcgg cgttgacgca ctcgtgttca ccggcggtat tggcgaaaac tcacgtaata
     2701 tccgagctaa aaccgtttcc tatcttgatt tcttgggtct gcacatcgac actaaagcca
     2761 atatggacaa acgctacggc aattcgggta ttatcagccc gaccgattct tctccggctg
     2821 ttttggttgt cccgaccaat gaagaactga tgattgcctg cgacactgcc gaacttgtcg
     2881 gcatcttgta gccaaaaaag ggacgagtcc gcacaatgcc gtctgaaacc ccaaacgccc
     2941 gattaggctg atgaggattt tagacggcat tgttcatttt tttgttatct tgcatttttg
     3001 tgcggacggt ggaatttcat cctgtaaaca taaatatttg tcggaaaaca gaaaccctcc
     3061 gccgccattt ctacgaaagc aggaaaccag caacgcaaag cgacagggat ttgttggaaa
     3121 tgaccgaaac cgaacgaacc ggattcccgc ctgcgcggga atgacgggat tttctgtttt
     3181 tgtggaaatg acgggatttt gaatttcggg cgtacaatac ggaaaacatg acgataagga
     3241 aaaaaactat ggcacagttt ttcgctattc atcccgacaa cccccaagaa cgcctcatca
     3301 agcaggcggt cgaaatcgtc aataaaggcg gcgtggtcgt ttatccgacc gattcctgtt
     3361 atgccttggg ttgcaaactc ggcgataagg cggcgatgga acgcatactc tccatccgaa
     3421 aaatcgattt gaaacaccac ctgaccctga tgtgcgccga tttgagcgag ttgggcacat
     3481 acgccaaaat cgacaacgta cagtttcgtc agcttaaggc cgccacgccc gggccttata
     3541 cctttatctt gcaggcgacg aaggaagtgc cggcgcgcac gctgcacccg aaacgcaaaa
     3601 ccatcgggct gcgtattccc gataatgcca ttgcacaagc cctgctgggg gaattgggcg
     3661 agccgctttt aagctgcacc ctgatgctgc ccgaagacgg cgaaccattg accgatcctt
     3721 atgaaatccg cgagcgtttg gaacacgccg tcgatttggt gattgacggc ggctggtgcg
     3781 gaaccgagcc gaccaccgtc gtcgatatga ccgacggcac ggaactggtg cgccggggtt
     3841 gtggcgatac ggcggtgttc ggtttgtagg gaaaccgatg ccgtctgaag catcggctgt
     3901 tcagacggca ttgcgcgcct tgccggtggc agtccgaaat gccggcgcgt atcgcgctcg
     3961 gtcggaatat ccgtttgaaa cggcattttg atgcattact gcaccgcaat cggaattctc
     4021 ggttcgtaga gcaggtcgta ggtcggcttg ttgagcaggt cttggagcgt gaaaccgtcc
     4081 agatacgtga aaaacgactt cattgcaccg ccgagtatgc ctgtcagacg gcaggacggt
     4141 gtaatcaggc attcgttgtt cgaacccata cattcgacca gctgcatcgg ttcgaggtgg
     4201 cggacgaccg agccgatgtt gatgcggtcg ggcggtgtgg caagccgcag accgccgcct
     4261 tttccgcgca cgctgtggag gaagccgcct ttgaccagcg cggtaacgac cttcatcaga
     4321 tggcttttgg aaatgccgta ggttacggcg atggtactga tgttgaccag cgcatcgtcg
     4381 ttgatggcag tgtagataag gacgcgcagc ccgtagtccg tatgttgtgt caaatacatg
     4441 attttctcgg tatggattgt tattcttatc ggtacggttt aaggttcacg gacaatacct
     4501 taatggttga aaccctgtcc gtcggggcgg tagaatgcag cctgtctgcg gcggtatgcc
     4561 gtctgaaaca tccgcgctac cgtttgagaa tttgttattg taactcaaaa tcatgaaacc
     4621 gttgaaacga catcccgccc ttatcgggct ttcgcgtgac caccaccatt cgctttccct
     4681 gtgcgtgcgt ctgttgcgga cgccggaaga aaggcatcgg gacgaactcg aaccgcattt
     4741 ttccgaattg gaaacccatt ttcgcgaaga agaaaccaag tttgccccaa tttggcagaa
     4801 tgtcgccccc gaattgaaac aacgtttcga gaaagaccac gcccgactgc ggcagatgat
     4861 ggcaagcccc gaatacggta acgcggcgtg gaataccgct tttgccacaa ccctgcgcga
     4921 ccacgcgcgc tttgaagaac gcgagctgtt tcccgccgcc gaaccgtttt tgccggcatg
     4981 attccgtttt gcggcaaata tattaatgat aaacaaggaa cacacatgaa atttaccaag
     5041 caccccgtct gggcaatggc gttccgcccg ttttattcgc tggcggctct gtacggcgca
     5101 ttgtccgtat tgctgtgggg tttcggctac acgggaacgc acgagctgtc cggtttctat
     5161 tggcacgcgc atgagatgat ttggggttat gccggactgg tcgtcgtcgc cttcctgctg
     5221 accgccgtcg ccacttggac ggggcagccg cccacgcggg gcggcgttct ggtcggcttg
     5281 actatctttt ggctggctgc gcggattgcc gcctttatcc cgggttgggg tgcgtcggca
     5341 agcggcatac tcggtacgct gtttttctgg tacggcgcgg tgtgcatggc tttgcccgtt
     5401 atccgttcac agaatcaacg caactatgtt gccgtgttcg cgctgttcgt cttgggcggc
     5461 acgcatgcgg cgttccacgt ccagctgcac aacggcaacc taggcggact cttgagcgga
     5521 ttgcagtcgg gcttggtgat ggtgtcgggt tttatcggtc tgattggtac gcggattatt
     5581 tcgtttttta cgtccaaacg cttgaatgtg ccgcagattc ccagtccgaa atgggtggcg
     5641 caggcttcgc tgtggctgcc catgctgact gccatgctga tggcgcacgg tgtgttggct
     5701 tggctgtctg ccgtttttgc ctttgcggca ggtgtgattt ttaccgtgca ggtgtaccgc
     5761 tggtggtata aacccgtgtt gaaagagccg atgctgtgga ttctgtttgc cggctatctg
     5821 tttaccggat tggggctgat tgcggtcggc gcgtcttatt tcaaacccgc tttcctcaat
     5881 ctgggtgtgc atctgatcgg ggtcggcggt atcggcgtgc tgactttggg catgatggcg
     5941 cgtaccgcgc ttggtcatac gggcaatccg atttatccgc cgcccaaagc cgttcccgtt
     6001 gcgttttggc tgatgatggc ggcaaccgcc gtccgtatgg ttgccgtatt ttcttccggc
     6061 actgcctaca cgcacagcat ccgcacctct tcggttttgt ttgcactcgc gcttttggtg
     6121 tatgcgtgga agtatattcc ttggctgatt cgtccgcgtt cggacggcag gcccggttga
     6181 gacaaaccgc cgcagatttc gggtctgggc ggtttgcttt tcagacggca gggcggtcag
     6241 ttgccgtcca gccagcggtc gcgtgtggtt ttggcttctt caaaatagcg gtacagggct
     6301 tcgcggtcgt cggtggtcag gatgtttgcc aaaacgtcca actgtttgcc caagccttga
     6361 accagttgca gcaggctgtc tttgttggca aggcagatgt ccgcccacac ggcgggatga
     6421 ccggaggcga tgcgggtgaa gtcccgaaag cccgtggcgg cgaatttcag atattcctgt
     6481 ccgtcggggt ggtcgagaat ctggtggaca taggcgaagg cggtcaggtg gggcatatgg
     6541 gagacggcgg cgaaaaccgc gtcgtggcgt tgcgcgtcca tcgtataaat ttccgcaccg
     6601 accgcgtgcc acaggttttc taccaaggca atgccgtctg aatgttcgcc gccgtgtggc
     6661 gtgatgatga gttttctgtg gcggaacagc ccgaactgcg cggcttgcgc accgcttctg
     6721 tccgaaccgg caatcgggtg ggcggcgatg cagcggtgca ggcggtcggg cagacagcgg
     6781 cggaaggctt cgatgaccga agatttggtg ctgccgacat cggaaatcca agtgtgttcc
     6841 ggcaaaacgg ggcgcagcgc ggtcaaaatg gcaggaacgg tggcgacggg tgtggcaatc
     6901 agtaccaagt ccgcaccgcc gatgctgtcc gcgtcgatgg caacggaagc ccggtcaatc
     6961 acgccgcgtt ccaatgcacg ttcgaggttg tcgcggtcgg tgtcgatacc ggtaacggtg
     7021 cggacgagtc cctgcctttt gaggtcgaga acgaacgaac cgccgatcag ccctacaccg
     7081 atgagggcaa tatggttcaa aatgggcatt tgtgtaaacg gttttcgcaa agtaccgtca
     7141 tggtagccta tcggcggaat atgccgcaag gtcggcagga aaaaggagaa gaaatggaca
     7201 aaatcagagt tgccgccgtg cagatggtgt cgggcgtgtc gccggaaacc aacgtcgccg
     7261 ccatgaaacg cctggtcgca cgggcggcgg agcagggtgc ggattgggtg ctgctgcccg
     7321 aatattgggt gctgatgggc gcaaacgata ccgacaaact cgcgcttgcc gagcctttgg
     7381 gcggcggacg ctttcagacg gcattgagcg aaacggcgaa agaatgcggc gtggtgctgt
     7441 tcggcggaac cgtgccgctg caaagccccg aggcgggcaa ggtcatgaat actttattgg
     7501 tgtacgggcg cgacggcaaa cggacggggc tgtaccacaa aatgcacctc ttcggctttt
     7561 ccggtttggg cgaacgctat gccgaagccg ataccatcag cgcagggcgg gaagtgccgc
     7621 acttgtcggc agaaggcgtg tcggtggcgg cgggcatttg ttacgatgtc cgctttcccg
     7681 aatttttccg ccgccagctg ccgtttgacg tattgatgct gcccgctgcg tttacgcaca
     7741 cgacgggcaa ggcgcattgg gagctgctgc tgcgcgcgcg tgccgtcgaa aaccaatgtt
     7801 acgtcgtggc ggcggcacag ggcggtttgc acgaaaacgg acggcgcacg ttcggacaca
     7861 gcatgattgt cgatccgtgg ggcgacgtat tggacgtatt acccgaaggc gaaggcatcg
     7921 ttacggcaga catcgatgcc aaccgcctga acagcgtccg caaccgcctg cccgccttga
     7981 aataccgggt tttggatgcc gtctgaaggt tcagacggca tcggtgccgg ggaatcagcg
     8041 ggctgtagga tgggcttcag cctgccattt ccatcaatcc cacatttcta ccgttttcat
     8101 cgaatccatc gaatccgccc ctttcgacga cccagtccta cgcaactgaa ccgtcattcc
     8161 cacggaagtg ggaatctaga atctcggggg tt
//
LOCUS       DAWFAN010000084         8147 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWFAN010000084 DAWFAN010000000
VERSION     DAWFAN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.084"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            235..1167
                     /gene="pip"
                     /locus_tag="WGO65_001318"
     CDS             235..1167
                     /gene="pip"
                     /locus_tag="WGO65_001318"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ0327609.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKDWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDIFPRLL"
     gene            complement(1224..2420)
                     /gene="bamC"
                     /locus_tag="WGO65_001319"
     CDS             complement(1224..2420)
                     /gene="bamC"
                     /locus_tag="WGO65_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ0327610.1"
                     /translation="MPSEAFRRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSQSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGDKNKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGQQAENALAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAITE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2429..3304)
                     /locus_tag="WGO65_001320"
     CDS             complement(2429..3304)
                     /locus_tag="WGO65_001320"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ0327611.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMTNDTILRLSEIPNIVGV
                     KEASGNIGSNIELINRVPEGFAVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGKCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            3342..3596
                     /locus_tag="WGO65_001321"
     CDS             3342..3596
                     /locus_tag="WGO65_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327612.1"
                     /translation="MLPIVAYFTAGVKSGGSGMHGAAWRRMRRVCIFVFEIRLLRFAN
                     DIPAATVRNLFAGRARMPSEIEIEAVWRIRYNPRFGFFNI"
     gene            3611..5002
                     /locus_tag="WGO65_001322"
     CDS             3611..5002
                     /locus_tag="WGO65_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ0327613.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(5064..5816)
                     /gene="rlmB"
                     /locus_tag="WGO65_001323"
     CDS             complement(5064..5816)
                     /gene="rlmB"
                     /locus_tag="WGO65_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ0327614.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(5882..6370)
                     /locus_tag="WGO65_001324"
     CDS             complement(5882..6370)
                     /locus_tag="WGO65_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HEZ0327615.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWLVGLISLAGILTDYVAGIWGTKYTGAGKLAVRGALIGSIIGMFFSLPGL
                     VLGPLIGAAAGELISCRNMIQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(6375..7094)
                     /locus_tag="WGO65_001325"
     CDS             complement(6375..7094)
                     /locus_tag="WGO65_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEZ0327616.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQTGAVQTFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMAQAHKARLLAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIKRVIYGAVEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRSVLSCFFQNKRKG"
     gene            complement(7098..8039)
                     /gene="miaA"
                     /locus_tag="WGO65_001326"
     CDS             complement(7098..8039)
                     /gene="miaA"
                     /locus_tag="WGO65_001326"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ0327617.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSHAERSFVPHHLIDIITPVQTYSAARFVEDCTHLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDGQ
                     RIGRALEVYYLTGKPMSTHLGSPTSHTLPFDLHTAALIPENRARLHENIALRFHMMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTHLIEAAKRFFGV"
BASE COUNT         1496 a         1898 c         2649 g         2104 t
ORIGIN      
        1 gtacggcgtt gcctcgtctt agctcaaaga gaacgattct ctaaagtgct caagcaccaa
       61 gtgaatcggt tccgtactat ctgtactgtc tgaggcttcg tcgccttgtc ctgatttttg
      121 ttaattcact atatcgtgat ttttcggcat ttgcctttcg gggcggcttg tgtttcgtgc
      181 gtgatgttgc gtgtgggaat gttcggattg tcagaggcaa tatgggagaa gatgatgtat
      241 gagataaaac agccttttca tagcggatac ttgcaggtgt ctgaaattca tcaaatttat
      301 tgggaagagt cgggcaatcc cgacggtgtg ccggtcattt ttttacacgg cggaccgggg
      361 gcgggggctt cgcctgaatg tcggggtttt ttcaatcccg atgtgttccg catcgtcatt
      421 attgaccagc gcggttgcgg acgttcgcgc ccgtatgctt gtgcggaaga caatacgact
      481 tgggatttgg tggcggatat tgaaaaagtc cgtgaaatgc tgggtatcgg gaaatggctg
      541 gtgttcggcg gttcgtgggg cagtactttg tcgctggctt atgcccaaac ccatccggaa
      601 cgggtaaagg gattggtgtt gcgcgggata tttttgtgca ggccgtctga aacggcgtgg
      661 ctgaacgagg cgggcggtgt gagccggatt tatccggaac aatggcaaaa atttgtcgcg
      721 ccgattgctg aaaatcggcg gaaccggctg attgaggcgt atcacgggtt gctgtttcat
      781 caagatgaag aagtgtgcct gtctgccgcg aaggattggg cggattggga aagctatctg
      841 atccgtttcg agccggagga agtggatgaa gatgcttatg cctcgctggc aatcgcgcgt
      901 ttggaaaacc attattttgt caacggcggc tggttgcagg gcgataaggc gattttgaac
      961 aatattggca aaatacggca tatcccgacc attatcgtgc aggggcggta tgatttgtgt
     1021 acgccgatgc agagtgcatg ggagttgtcg aaagcctttc ccgaagcgga attgagggtg
     1081 gttcaggcag ggcattgcgc gttcgatccg cctttggcgg atgcgttggt tcaggcggtt
     1141 gaggatattt ttcctcggtt gttgtaaaaa gttccgcata aaaaagcagc ttctgtttgg
     1201 aagctgcttt tgttttgaac ggtttaacgc agttcggaat ggagtttgcc caacagtgcg
     1261 gatgcgtctt tgccggcata tgcgctgccg tctttgttga gcaggacgat gcgcgagccg
     1321 ttggcgacag gctcggcata gacaatcagt tccggctgtt cggcaggttt ctccgctttg
     1381 cctttgccca gcaggcgttt gaacaggccg ggtttttgtt cggtaattgc attgctttcg
     1441 ttcggggctt tttgaaccaa gaaggcgtgg cgttcggtgt tttgaccgac gacggtcagc
     1501 ccgatgcggt cgagggcgag cgcggtgcgc cgccagtttc tgccgtagtc gccaaagaca
     1561 atcaggcttt tgccttcgat acgcgccatt tcgttggcgg cgggaagggt cggttttttt
     1621 gccaatgcgt tttccgcctg ctgcccgtca acgcccaaat attgcataaa gcgcgtcagg
     1681 aatgccgctt cgaggttggg gtcggaagcg gaaggctgcc acatggtcgt gtctttgttt
     1741 ttgtcgccat acacttcttt catcgctttg tgggcgaaga agatgtcgga aacgccgttt
     1801 ttgccctgtt cgatacggac gatgaatttg tcgcgctcgc cggtggagta gatgccgccc
     1861 aaaccgactg tgtcgaatag gcggcgcaag ctgtcttggg ggattttggc acggttttcc
     1921 gcccactcgg tttccatttg tccgatggca ggttcttcgg atttgatgtc gaagccgttt
     1981 tcctgccaaa aggctttcag gagcggccat atttccgccg gtgatttgcc gtcgacaacg
     2041 agccagcgtt ggctgccgtc gcgctcgagg cggacacctt tgacgctttt caatacttcg
     2101 gcatcggcag gctgttggac ggcgggtgtg cggcgttttt ccaaatcgct ggcgcggacg
     2161 gctcccgaac cggcaggcag gcggtagagg ttgccttggt cggggttgtt caaatcgggt
     2221 gggacttcga gtttgatcag gcggtgcgac tggctttggt agtcgagctt gggctgttcg
     2281 gttttgctgc cggagcaggc ggcaagcccg atgagtgcga gcgcggcaat gacgggtttg
     2341 atatgggtca tcgtgtcatc ctgtgtgatg gatattaaag tgtttgttgc gttatgccgt
     2401 ctgaacgctt cagacggcat ggctatattt aaagttgtcc cgaggctttc agggcggcgc
     2461 ggacttttgc ctgtccgcct tccgtcagag gaacgagcgg caggcggacg tgcggttcgc
     2521 atttgcccag ggcggatacc gcccatttcg gtgcggcggg gctgggttcg cagaacatgg
     2581 tgtcgtaaat cggaatcagc cggtcattga gttcgcgtgc gagggcaatg tcgccttgca
     2641 gtgcggcgcg gcacatatcg gcaaagagct tgggtgcggc gttggcggct acggtaataa
     2701 cgccgtgtcc gccgcacagc atgaacggca gggcggtatg gtcgtcgccg gaaaggacgg
     2761 cgaagccttc ggggacgcgg ttgatgagtt cgatgttgct gccgatgttg ccgctggctt
     2821 ctttcacgcc cacgatattg gggatttctg aaaggcgcag gatggtgtcg ttagtcatgc
     2881 tgacgacggt acggccggga acgttgtaga gtatcatcgg aatcgcggcg gcttcggcaa
     2941 cggctttgaa atggcggtac atgccttctt gcgaaggttt gttgtaatag gggacgacgg
     3001 agagggtgta gtccgcgccg gctttttcgg cggcttggga aagggcgatg gcttcgacgg
     3061 tgttgttcgc ccctgtgccg gcgatgacgg ggacgcgttt ggcaacgtgt ttgacgacgg
     3121 cttcgataac ggcggtgtgt tcttcgacgg agagggtggc ggattcgcct gtcgtgccga
     3181 cggcgacgat gccgtccgtg ccgttttcaa tgtgccagtc gattaagtcg cggagttgtt
     3241 cgtaatggat gctgccgtct tgattcatcg gggtaatcag ggcaaccagg ctaccttgta
     3301 acatacagaa ccttttatca gttgtggtgt agggggcggt aatgcttccg attgtagcct
     3361 actttaccgc aggtgtgaaa tccggcgggt ccggtatgca cggggcggct tggcggcgga
     3421 tgcgtcgggt ttgtattttt gtgtttgaaa tcaggttatt gcgttttgca aacgatattc
     3481 cggcggcgac ggttcggaat ctgtttgccg gacgggcgcg tatgccgtcc gaaatcgaaa
     3541 ttgaagcggt atggcggatt cgctacaatc cgcgctttgg ttttttcaac atttgaagca
     3601 aggaaacatt atgagcggtc atatgggaaa aggggcggat gcgcctgatt tggtgtacgg
     3661 tttggaggat aggccgccgt tcggtaatgc gctgttgagc gcggttaccc atcttttggc
     3721 gattttcgtg ccgatgatta cgcccgcgct gattgtgggc ggcgcgctgg aactgccggt
     3781 ggagatgacg gcgtatctcg tgtcgatggc gatggttgcg tcgggtgtcg gcacttattt
     3841 gcaggtcaac cgcttcgggc cggtcggttc ggggatgctg tctatccagt cggtgaattt
     3901 ctcgttcgtt accgtcatga ttgcgctggg cgcggggatg aaagagggcg gtttgactaa
     3961 ggatgcgatg atttcgacgc tcttgggcgt atcgtttgtc ggcgcgtttt tggtgtgttt
     4021 ctcggcgtgg cttctgccgt atttgaaaaa agtgattacg ccgacggtca gcggtgtggt
     4081 ggtgatgctg atcggcttga gtttggtaca cgtcggtatt accgatttcg gcggcggctt
     4141 cggcgcaaag gcggacggca cgttcggctc gatggaaaac ttggggctgg catcgctggt
     4201 gctgctgatt gtgctggtgt tcaactgcat gaaaaacccg ctgctgcgga tgagcggcat
     4261 tgcggtcggt ctgattgccg gctatatcgt cgcgctgttt ttgggcaagg tggatttttc
     4321 ggcactgcaa aacctgccgc tggttacgct gcccgtaccg tttaaatatg gttttgcttt
     4381 tgactggcac gcatttattg tggcgggtgc gattttcctg ttgagcgtgt ttgaggcggt
     4441 cggcgatttg accgcaacgg caatggtgtc cgatcagccg attgaaggcg aggaatacac
     4501 caaacgcctg cgcggcggcg tgttggcaga cggcttggtg tcggtgattg cgacggcttt
     4561 gggttcgctg ccgctgacaa cgtttgcgca aaacaacggc gtgattcaga tgaccggcgt
     4621 ggcttcgcgc catgtgggca aatatattgc cgtgattttg gtgctgttgg gtctgttccc
     4681 cgttgtcgga cgcgcgttta cgacgattcc gagtccggtt ttaggcggcg cgatggtttt
     4741 gatgttcggc ttgattgcga ttgcgggtgt gcggattttg gtcagccacg gcatccgcag
     4801 gcgcgaggcg gtgattgcgg cgacttcggt cggcttgggc ttgggcgtgg cgtttgagcc
     4861 ggaagtgttt aaaaacctgc ctgtcttgtt ccaaaactct atttccgccg gcggcattac
     4921 ggcagtcttg ctgaatttgg tcttgcccga agataaaacc gaggcggcgg tcaagtttga
     4981 taccgaccac ttggaacact gattttgaaa acgaatgccg tctgaaacag aatccctgtt
     5041 tcagacggca ttgtttttga ggcttacgct ttttcgtttt ttaatacgcg ctgccggcgg
     5101 gtttcgctta ataccattcc ggcagacacg gagacgttca tgctttcgac cgtgccgaac
     5161 atgggtatgg acaccagcat gtcgcaatgt tcgcgtgtca ggcggcgcat accgtcgcct
     5221 tcgttgccca tcacccatgc cgcgctgtcg ggcagatcgc aatggtaaag gtcggcgttg
     5281 ccgcccatat cggtgccgat gatccaaatg ccgtattctt tcagctcgcg cagggtgcgg
     5341 gcgaggttgg ttacggtgat gtaggggacg gtttccgccg cgccgctggc gactttgctg
     5401 acggtggcgt tcagccccgc gcttttgtct ttcggtgcga tgacggcatg tacgcccatt
     5461 gcgtcggcgg tacgcaggca cgcgccgagg ttgtgcggat cggtgatgcc gtcgagtatc
     5521 agcagcagcg gcggttcgct taagttttcc aatacgtctt cgaggtggac gtggtttttg
     5581 gaggcatcga taaatccgac cacgccctga tggcgcgcac ctttgctgat ggcgttgagg
     5641 cggtcggcat cggcaaaata tacgcggatg ttttcgtttg ccgccttttc caacacttcg
     5701 cgcgtgcgta catcgttttt gccttcttgg acatagagtt cgacgatgga tttggggttt
     5761 tgccacaatc gggcgttgac ggcatggaag ccgtagatag gtctttggtt tgccatatgg
     5821 tgctttgtaa aaagggttca gacggcatta tagcaatttg ccggtatgcc gtctgaaggg
     5881 gttaaaacag gtaggcgatg tatttcacca acaggataaa caagatggat acggcgcagc
     5941 cgattttgaa ggccgttccg acgacaagcc ccaacagcgt acccaagccc gctttacctg
     6001 cctgtatcat gttgcggcag ctgatgagct ctcctgcagc cgcaccgatt agcgggccga
     6061 gtacaaggcc gggaagggag aaaaacatgc cgatgatgct gccgatcaat gcaccgcgaa
     6121 cggcgagctt gcccgctccg gtatattttg ttccccatat acctgccaca tagtccgtca
     6181 gtatgccggc aaggctgatg agtccgacca gccacaggat gcctgcgccg tagatttggt
     6241 atccgccggc ataggcaagc agccatgttc cggcaaacat caatgctaat ccggggaggg
     6301 cggggtaaac gatgcccgcc gtaccgacgg ctatcagggc gagggcgagg atgacgagca
     6361 aggcggtcat aggttcaacc ttttcttttg ttctgaaaaa aacagcttaa tacggagcgg
     6421 cattcttctt gcaggattcc gccccgtatg gcggtgtgcg tattgaggcg tttgtcggca
     6481 aacaggttga cgatgctgcc tgctgcgcca gttttgggtt ctaccgcacc gtatatcacg
     6541 cgcttgattc gtgcctgtat cagtgcggac gcgcacatgg cgcagggttc gagggtgata
     6601 tatatgtcgc atccgtcaag gcggtagttt tgcatttcgc tgcctgcctg tgccaaggcg
     6661 ttgatttcgg cgtgtcggct gacattgcag tcggcaatgc aggtgttgtg tgccgatgcg
     6721 atgattttgc cgtcggaaac gatgactgcc ccgacgggta tttcgccgtc ggcggcggat
     6781 tgttctgctt ggcgcagtgc ttcgcgcatg aagtgttcca tttcttcctg cggcggaaag
     6841 gcggcgacgg gcggatggtt tttcaattcg gcaagaaggc gggctttatg cgcttgcgcc
     6901 atttcttgcg gcggtgtgcc gtccagcagc gactcgagtt gccacagcgt gcttttcgtg
     6961 agggtcaaac ctgatgcctt caataacaag aaggtttgaa ccgcgccggt ttgacgcagt
     7021 tcttcgagcg tacggatacc gagcctgtgt agggcggcga cggttttggg ggcgagcggc
     7081 ggcgtggtca gcatagttta tacaccgaaa aaccgttttg ccgcctcaat cagatgtgtg
     7141 cctgaagtgc cgtctgaaaa cgggtcggca acgcagtcta aaggtgtttt gcgcaaccaa
     7201 gtcagttggc gtttggcaag ttggcgggtg gcggcaatgc ctttctcgac aaatgccggg
     7261 aaatcggttt ttccatccag atatttccat gcctgacggt agccgacgca gcggatggcg
     7321 ggggagtcgg cggtcaggcc gggatagcgg cggcgcaggt tttctacttc gccgataaag
     7381 ccctgttcga gcatcatgtg gaaacgcagg gcgatgtttt catgcaggcg ggcgcggttt
     7441 tcgggaatca gggcggcggt atgcaaatca aaagggagcg tatgggaggt tgggctgccg
     7501 aggtgcgtac tcatcggttt gccggttaaa taataaactt ccaaagcgcg tccgatacgc
     7561 tggccgtcgt tcggtttcag acggcatgct gtttcagggt cgactttttg cagggtgcgg
     7621 tagagaaaat ccaagccgta catttgtttt tgttcgtcca agtcggcacg caggcaggca
     7681 tcggcttcgg gcaaatcgtt caaaccttgg gtcagggcgc ggaaatacat catcgtgccg
     7741 ccgacaatca gcggatattt cccacgcgcc gtgatttcgc caatcagatg cgtgcagtct
     7801 tcgacaaaac gggcggcact gtatgtctga acaggcgtaa tgatgtcgat aaggtggtgc
     7861 ggtacaaagg aacgctcggc atgagacggt ttcgccgtgc cgatgtccat atcgcggtaa
     7921 accagcgcgg aatcgaggct gatgatttcg acaggcaggg tttcggcaat tttgagggcg
     7981 agcgcggttt tgcctccggc ggtcggcccg agcagggcaa aggctttcgg ggtcggcatg
     8041 atgtttcagg gttgcgaaaa tgcggattat agcggaaagc gtgccgacgt tatattttag
     8101 tttgcggaag cacgccgacg gcaagggggc gtgtttaccg tatgcct
//
LOCUS       DAWFAN010000085         8028 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWFAN010000085 DAWFAN010000000
VERSION     DAWFAN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.085"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(303..2339)
                     /locus_tag="WGO65_001327"
     CDS             complement(303..2339)
                     /locus_tag="WGO65_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ0327618.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     TRTDWANTVERLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVVRPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPRIDALLRHSGFDNAA"
     gene            complement(2427..3299)
                     /locus_tag="WGO65_001328"
     CDS             complement(2427..3299)
                     /locus_tag="WGO65_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0327619.1"
                     /translation="MEIWNRLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3569..5959
                     /gene="gyrB"
                     /locus_tag="WGO65_001329"
     CDS             3569..5959
                     /gene="gyrB"
                     /locus_tag="WGO65_001329"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ0327620.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            6058..7443
                     /locus_tag="WGO65_001330"
     CDS             6058..7443
                     /locus_tag="WGO65_001330"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ0327621.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLVGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETKIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(7514..7612)
                     /locus_tag="WGO65_001331"
     CDS             complement(7514..7612)
                     /locus_tag="WGO65_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ0327622.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
BASE COUNT         1849 a         2219 c         2233 g         1727 t
ORIGIN      
        1 cctgattttt gttaatccac tataaaatgc cgtctgaagc gttgtttgac cgtttcagac
       61 ggcattttta tcaaattcaa aaatattccg tcattcccgc gaaagcggga atctagaacg
      121 taaaatctaa agaaaccgtt ttatccgata agtttccgca ccgacagacc tggattcccg
      181 cctgcgcggg aatgacggga ttttggctta ctgtttttgt gaaaatggcg aaaccgtgca
      241 aatgctgatg ccgtctgaaa cactgtttga ttgtttcaga cggcattttt tgttcacatc
      301 gatcaagccg cgttgtcgaa accgctgtgg cgcaagagtg cgtctatgcg cggttcgcgt
      361 ccgcggaagg ctttgaagga ttctgccgcg ctgcgcgatc cgccgacggc gaggatttcc
      421 tgccaaaagc gtttgcctgt ggcggcgaca tcgtcgcttt cttcaaaggc ggcgtatgcg
      481 tccgcgctca atacttccgc ccatgcgtag ctgtaatagc ctgcggagta gccgccggcg
      541 aagatgtggc cgaagctgtt ggcgaagcgg ttgtattcgg gcggtcggac gacggcgact
      601 tctttgcgca cgctgtctaa aacctgttgc cagtttttca gacggccttc gtcgtcttcg
      661 ctgtaaatca tcatatcgaa gagggcgaac tccatctggc ggacgaggaa cattccgcgt
      721 tggaagtttt tggcggcgag cattttgtcg aagagttctt tcggcagggg aacgccggtt
      781 tcttcgtggg cggacatttg tgccaagaca ttgtattccc agacgaagtt ttccatgaac
      841 tggctgggca gttcgaccgc gtcccattct acgccgttga tgccggatac gcccagttcg
      901 tccacttggg taagcaggtg gtgcagcccg tgtccggttt cgtggaagag ggtgaggatt
      961 tcgtcgtggc tcaaacgcgc ttctctgccg ccgacgggcg gggcgaagtt gcagacgagg
     1021 taggcggtgg gcagttgcag cgtgccgtct gaaaaacggc ggcgaccttt gtagtcgttc
     1081 atccacgcgc cgccgcgttt gccttcgcgt gcgtacaaat ccatataaac gccgcctatg
     1141 gtttcgccgt tttgttgcaa ttcaaaatag cgcacgtctt tgtgccagac ggggacggtt
     1201 ttttcggtaa atccgatgcc gtaaagtttt ttgatttggg cgaacagtcc gtttaatact
     1261 ttgccgacgg ggaagtattt tttgacttcg gtttcgctga acgcgtattt ggcttcgcgc
     1321 agtttttcgc cagcatagcc caagtcccac ggttgcaaat cggcgagacc gaggctttcg
     1381 cgggcgaagg ctttgacttc ggcgaggtct ttttcggcgt agggtttggc gcggcgggcg
     1441 aggtcgtgca ggaagtttaa aacttgttcg ggggtgtccg ccattttggt tgccagcgac
     1501 aattcggcgt agtttttgaa gccgagcagt ttggcggttt gcagggcgtt tgcgagcgtg
     1561 cggtcgatgt tggcggtgtt gtcgaatttg ccgtcgtctg aaagttcgct ggcgcgggta
     1621 acgtaggcgc ggtagatttg ttcgcgcagt tcgcggttgt cggcgtattg gatgacggcg
     1681 aggtagtgtg gaatctgcaa gccgattttg tagcctgttt tgctttcgct ttgcgcggcg
     1741 gcggcaaaca tggcgagcgc gtcttcggga atgccggcaa gcggtgcggc atcgtcaaag
     1801 taaatgccga acgcgtcggt cgcgtctagg acgttttggg agaatttggc ggaaagttgc
     1861 gcgccttcgg tttgcagttt tgccagttct gcctgctgtt cgggcggcag ttccgcgccg
     1921 ctgaggacga aatcgcgcag atcgtggttg agtttggttt tttgtgcggg ggagagggtg
     1981 tcgaattcgg gggaattttt gatggttttg aagcggttgt acagctcgat gtcttgtccg
     2041 atttcggtga agaagacggt gatttcgggc atcagttcgt tatagacggc gcgcagttcg
     2101 ggcgtgtcgg cgacggagtt gaggtgcgac accacgcccc aaatcctgcc gacgcgctcg
     2161 gtgatgccgg tcagacgctc gacggtgttc gcccagtcgg tgcgcgtttg ggctttgatg
     2221 gcggcgattt gttcgcgcgc ttcggcgatg gcggtttgca gggcgggttt gatgtcttcg
     2281 gttttgattt ggtcgaaacg gggttcttcg cccaagtgga gcagtgcgtt gtcagtcata
     2341 agatgggttt cctttgcgtg ttgtgcttca gacagcatat cggcaatatg ccgtctgaag
     2401 ccggaaatgg ggtcggtcag ggaaagttaa agtgtttcgg agccggcggg gtaatttaac
     2461 aggcggtatt gtacgcgcaa aaattcgtcc ataaccgcct gttggatttc cagccgcttt
     2521 gaaacggggg aggcgaagcg gacgatgatg cggtatgcct tgtcatcgta cggcacgcgg
     2581 gtaacgcgcg gtctggcggc gggcgtgata aacagttttt ccgcctgcac gttttccaaa
     2641 tgccgttgga tggcggggat gtagggcgcg cacaagggct cgagtacggc tttcagacgg
     2701 catacggctt catccgaatc caaatggatg ggaacgggga tttcgaccgt atggatgaca
     2761 tagtcgccca aaatattgtc gcggcgcacg gggtggctca acaacaggct gttggggaaa
     2821 gaaacggtgg ttcccgcaag ctgtccgacc agggggttcg gaccgacctg catcatcagc
     2881 gtgttcaaca ggttgatgtc gaccacgcgc ccgcgcaggc cgttgatttc gatatagtcg
     2941 ccgaccgagt attgctgggt ggcagacctt aaaatactgc ccgacagaca cataatcagt
     3001 tcctttgtcg ccacgacgac cgccgccgcc accgcaaaca tcgacaaagc cagcgtttgg
     3061 atttgcgccg accagataaa tgccagcgaa aacagcacca aaagcagcgt tatattgcgg
     3121 ctggcaacca aaaaccgccg cttgctttcg atgccgaaat ccggatgccg tttgaagtgg
     3181 atattcaaca gaagggcgcg cgccagcagc aaagccgcaa ccgccgccac ggattcgacc
     3241 gcctccgcac gtatcgggac ggcaccgagc caagtgtcca acctattcca tatttccatt
     3301 tccgcgcccc tgtccgaaaa gtatagggtg tagattttag tggcaaaaaa acgcttttgc
     3361 cactttacgg gacaatcccc gacctgatgc cggaggcaaa cgtaccgttt catcttttcc
     3421 cggcggcaca ctgatgcgga taacgctgaa ttaggcggca aaatcggcta taatacacaa
     3481 ttatatatgt cgtcccgccc gctttttcta caaggtgcgg acttgttttg gcggcatcgc
     3541 aaaatctttt caaaatccgg caaaaaatat gactgaacaa aaacacgaag aatacggcgc
     3601 cgacagcatt caggtgctcg aaggcttgga agcggtacgc aaacgccccg gcatgtacat
     3661 cggcgacacg caggacggca gcggcctgca ccatatggtg tttgaagtat tggacaacgc
     3721 catcgacgaa gcgctcgccg gacattgcga caaaatcacc gtaaccatcc atgccgacca
     3781 ttccgtcagc gtggccgaca acggccgtgg catgccgacc ggcatccacc cgaaagaagg
     3841 ccgctccgcc gccgaagtca tcatgaccgt cttgcacgca ggcggtaaat tcgacaacaa
     3901 cagctacaaa atctccggcg gcttgcacgg cgtgggcgta tccgtcgtca acgcgctgtc
     3961 cgactgggtt acgctgacca tctaccgcga cggcaaagaa cacttcgtcc gcttcgtgcg
     4021 cggcgaaacc gaagagccgt tgaaagttgt cggcgattcc gacaagaaag gcacgaccgt
     4081 acgcttcctc gccagcacgg aaactttcgg caacgtcgaa tacagcttcg acatcctcgc
     4141 caaacgcatc cgcgaacttt cgttcctgaa caacggcgtg gacatcgaat tgaccgacga
     4201 gcgcgacggc aaacacgaaa gcttcgccct ttccggcggc gtggctggat tcgtgcaata
     4261 catgaaccgc aaaaaaacgc ccttgcacga aaaaatcttc tacgcgttcg gcgagaaaga
     4321 cggcatgagc gtcgaatgcg caatgcaatg gaacgacagc tatcaggaaa gcgtgcagtg
     4381 cttcaccaac aacatccctc agcgcgacgg cggtacgcac ctgaccgcgt tgcgccaagt
     4441 gatgacgcgc accatcaaca gctacatcga agccaacgaa gtcgccaaaa aagccaaagt
     4501 ggaaaccgcc ggcgacgata tgcgcgaagg tttgacctgc gtgttgtccg tcaaactgcc
     4561 cgaccccaaa ttttcgtccc aaaccaaaga caaactggtt tccggcgaaa tcggtcccgt
     4621 cgtcaacgaa gtcatcagcc aagccctgac cgacttcctc gaagaaaacc ctgccgaagc
     4681 caaaatcatc accggcaaaa tcgtcgatgc cgcccgcgcg cgcgaagccg cccgcaaagc
     4741 ccgcgaaatc acccgccgca aaggcgtaat ggacggcttg gggctacccg gtaaactcgc
     4801 cgactgccaa gaaaaagacc ccgcattgtc agaactttat ctggtcgagg gcgattctgc
     4861 aggcggttcc gccatgcagg ggcgcgaccg caaattccaa gcgattttgc cgcttaaggg
     4921 caagattttg aacgtcgaaa aagcgcgctt tgaaaaaatg ctcgccagtc aggaagtagc
     4981 cgctttgatt accgcgttgg gcgcgggcat cggcaaagaa gaattcaatg ccgagaagct
     5041 gcgttaccac cgcatcatta tcatgaccga tgccgatgtg gacggcgcgc atatccgcac
     5101 cttgcttttg accttcttct accgccaaat gcccgaactg gtcgagcgcg gctacatcta
     5161 tatcgcccag ccgcctttgt ataaagcgaa atacggcaaa caggaacgtt acctcaagga
     5221 cgagttggaa aaagaccaat ggctgctcgg tcttgccttg gaaaaagcca aaatcatttc
     5281 agacggccgc accatcgaag gcgcagaact tgccgacacc gccaaacaat tcctgttggc
     5341 aaaaaccgtc atcgaacagg aaagccgctt cgtagacgaa ctcgtcctgc gcgccatgct
     5401 acacgcgtcg cccattgatt tgacgtcgtc tgaaaacgcc gataaagccg ttgccgaact
     5461 ttccggtctg cttgacgaaa aagaagtcgc cctcgaacgc atcgaaggtc atgaaggaca
     5521 ccggttcatc aaaatcacgc gcaagctgca cggcaacgtc atggtcagct acatcgaacc
     5581 caagttcctc aacagcaaag cctaccaaac cctcacccaa accgccgccg cgctcaaagg
     5641 catggtcggc gagggtgcca agctttacaa aggcgaaaac gggtacgacg cggacagctt
     5701 tgaaaccgct ttggacatct tgatgagcgt tgcccaaaaa ggtatgtcca tccaacgata
     5761 caaaggcttg ggcgagatga accccgagca gctgtgggaa accacgatgg atcccgccgt
     5821 gcgccgcctg ttgaaagtgc gcatcgaaga tgccattgcc gccgacgaag tgttcgttac
     5881 gctgatgggc gacgaggtcg agccgcgccg tgcctttatc gaaaacaacg cgctgattgc
     5941 ccaaaatatc gacgcataag tgccgtttta aaaaaggaga cgggcatcgt gccgcgtctc
     6001 cttttggttt gtcaaacgga agccatgccg tctgaaaccg cctttcggag caaaaccatg
     6061 gtcagcattt ttgatatttt caaaatcggt atcgggcctt ccagttcgca tacggtcggc
     6121 ccgatgaagg cagccgccgc ctttgcggca ggtttggatg cacaggctgt tcgcatcgtc
     6181 atcgacattt acggctcgct cgccctgacc ggatacggac acggtacatt tgacgcgctg
     6241 atgctcggtt tggaaggcag cctgccgcac gacatcccgc ttgtcggcat tcccgaacgc
     6301 ctcgaacgca tccgcacgca gcacatcctc cggctcaacg ggcaagaaat ccgcttcatc
     6361 cccgaccgcg acctgaacat actcggcaat caagtgctgc ccaaacaccc caacggcctg
     6421 cgttttaccg cctatgcttc agacggcacg gtattgaatg aacaggttta ttattcggtc
     6481 ggcggcggct ttgtcgttac cgaagaagat tttgaccggc aggcggaaac ggaaaaagcc
     6541 gttccctatc cctataccag ttgcgccgaa ctgcttgccc gatgccgtct gaaccggctc
     6601 gacatctccg aagtcgtgtt ggcaaacgaa gccgcgcttg ccggatgcgg cgaagccgaa
     6661 atccgccgcc gcgccgctgc cgttgccgag gttatggaag gctgcatcaa acgcggcttg
     6721 ggtgcggacg gcgaactgcc cggcggattg aacgtccgcc gccgcgcccc gcagcttgcc
     6781 gccaagctca aagtcctgcg cgaaaccaaa atcgtcaaca cccagctctg gccgatggtg
     6841 tacgccatgg cggtcaacga agaaaacgcc gccggcggac gcgtcgttac cgcaccgacc
     6901 aacggcgcgg caggcatcat tcccgccgta ttgcactatt tccgcaagtt caatccgcac
     6961 gccacacagg aacgcgtcga aaacttcctg ctcaccgcag gcgcaatcgg catcctctac
     7021 aagaccaacg cctccatttc cggtgcggat gtcggctgtc agggcgaagt cggcgtagcg
     7081 tgttcgatgg cggcgggcgc atacgccgaa gtcatcggcg gcacgcccaa acaagtggaa
     7141 aacgccgccg aaatggcgat ggaacaccat ttggggctga cttgcgaccc cgtcggcgga
     7201 ctggtgcaaa tcccctgcat cgagcgcaac ggcatcgccg ccgaaaaagc cctcaaactc
     7261 ggcaccctcg cgctcttgga agacggcacg gacaaaaaag tctcgctcga cgaagtcatc
     7321 cgaaccatgc tgcaaaccgg ccgggatatg aaggcgacct acaaagaaac ctcgcttgcc
     7381 ggactcgccg ccacgctccg gaaaaaagcc gtccccgtat ccgtgcgcgt ggtcgagtgc
     7441 tgaggatgga cagaaacgaa aatgccgtct gaaaacagtt ttttagacgg catttcattt
     7501 atttcttgcg gctttaccgt ttcagtgcca gcgtcaacac gcccgccgta accagcccca
     7561 agcctatcca ttcctgcgtg ttcgggcgtt cgtctaagaa aaccaccgcc atcaggaatg
     7621 caaaccggcg ttgtttgcag catccggcaa gaatttttaa aaggtctttt ctaaaatatg
     7681 cagatattcg aatgtcgaat ttgccttatg attacggttt tctgtcttat ctgccttaaa
     7741 ccgccggtat tccgtttgaa ccaaatcata tttgccgaaa cgctcaaaaa tctcccttac
     7801 ctgcccgaca gacatcaagc cttcgttgtt gtagctgaga aaatatattg aaagtccgca
     7861 tccctaatca aagcctcaaa taccggaatc acatgcgatt ttgagcaagt ccgcgctaaa
     7921 acacatcgta cagcgcaaaa ccggatggga atggcaaaat ccggaaacaa acatttttca
     7981 aagcatacaa acaaaaaaat accaaccgga ggttggtatt ccttaact
//
LOCUS       DAWFAN010000086         8020 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWFAN010000086 DAWFAN010000000
VERSION     DAWFAN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.086"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(68..814)
                     /locus_tag="WGO65_001332"
     CDS             complement(68..814)
                     /locus_tag="WGO65_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0327623.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WGO65_001333"
     CDS             complement(993..1664)
                     /locus_tag="WGO65_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ0327624.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WGO65_001334"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WGO65_001334"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ0327625.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLMMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WGO65_001335"
     CDS             complement(2481..2963)
                     /locus_tag="WGO65_001335"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ0327626.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WGO65_001336"
     CDS             complement(2995..3684)
                     /locus_tag="WGO65_001336"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ0327627.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPIADTLKCADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIHPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WGO65_001337"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WGO65_001337"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ0327628.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVAHTETKRSGKLKVIRADENELAEHG
                     RYLDGLGEACIWRKTQMPSENGGTEA"
     gene            complement(4571..5881)
                     /locus_tag="WGO65_001338"
     CDS             complement(4571..5881)
                     /locus_tag="WGO65_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0327629.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WGO65_001339"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WGO65_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ0327630.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="WGO65_001340"
     CDS             complement(6056..6355)
                     /locus_tag="WGO65_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327631.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="WGO65_001341"
     CDS             6706..7902
                     /locus_tag="WGO65_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0327632.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAAVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1886 a         2405 c         1958 g         1771 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaagg aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccatataccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcac gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacagggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatctctga accggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tattccgttt tcgagtacgc cggtgatttt gttgatttcg tcttccatca
     1861 tcagcggttt gtcgatattc aagccgtgga catcgacgat ttggttgccg taaaaagtgg
     1921 tgtagccgat acgcaattca ggctgtccgc ccatagcgag cagtttgcgc gaaacaaggg
     1981 aacgcgcgct ttcgacgact tccacgggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcgtc cgcaatgcag acgaattttt ctgatgcgct ggcgacgatt ttttcgttga
     2101 ggtgtgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 caatatagac cgccaacccc gatacttcgt tcagggaaac gacgggaata tcatattggg
     2221 caagcagttc actggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgagccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatccgt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggtggat gcccaatttt tcgacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgcgcctgcc aaaggctcgt ccgctcgacg gttgcactaa tgttcccacc
     3241 gtccgcgcac ttgagcgtat cggcaatggg aattgccaaa atcccacctt cggcagcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcaac gcgcggcatc
     3361 gtgtaccaga atattgtcgg tttccgccgc caaaccggtt tccaacagtt ttgccacacc
     3421 gttgcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaag cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatac tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagggcgata tttttgcgtt tcatgcctcc gtcccgccgt tttcagacgg catctgcgtc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatc gcccgtgctc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcgccacg
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaattg ccgccgcgtc
     3901 atcgctaaat aaacttcgcc caaaagctcg cagtcgatca gcgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctctatc gtcagaccgt gcaccctcgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca gaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tttctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc agcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgctg ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcaggc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgata gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc gtttcacgca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg ggtatcggca gccttggcgg
     5281 gtacggacgg cataaacagg ctgccgaccg tgcctcctac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat acccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgttagaat agcctgcttc ttttatcaac cgtttcagac
     6601 ggccccacta ctttcccgcc taggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc catcaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaataaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgcc
     7801 gccgtgatta ccactgccga cagcaaagcc gttgccgtgg tcattccgac taacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggtctgt aaggttttat ccgcacacga
     7921 actgcctccg gaaatggagg cagttttttt atccggcttt ccatgcttaa acagcactgc
     7981 ctcttttcag acattgacgg ttgcagccgc ttacctgaac
//
LOCUS       DAWFAN010000087         7983 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWFAN010000087 DAWFAN010000000
VERSION     DAWFAN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.087"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(263..2953)
                     /gene="glnE"
                     /locus_tag="WGO65_001342"
     CDS             complement(263..2953)
                     /gene="glnE"
                     /locus_tag="WGO65_001342"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ0327633.1"
                     /translation="MSDNRLDAARRHSLFLARNLDSGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDAIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRDTKSPQHLSVVAMGKAGGYELNVSSDIDLIFTYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGNSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAELGIMP
                     SENVETLLAAYRFLRDIEHRLQYWDDQQTQTLPTSPEQQQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAEQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALSGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLANYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWRDVFGEEAATA"
     gene            complement(3151..3354)
                     /locus_tag="WGO65_001343"
     CDS             complement(3151..3354)
                     /locus_tag="WGO65_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327634.1"
                     /translation="MGFWNGVAKAVGEGMIEAANEHKALKMEYAEKSSEELHEIVKSD
                     GFFKNSTREKGAAYAVLKERGEV"
     gene            complement(3491..3964)
                     /locus_tag="WGO65_001344"
     CDS             complement(3491..3964)
                     /locus_tag="WGO65_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ0327635.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4017..4298)
                     /locus_tag="WGO65_001345"
     CDS             complement(4017..4298)
                     /locus_tag="WGO65_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327636.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4385..4708)
                     /locus_tag="WGO65_001346"
     CDS             complement(4385..4708)
                     /locus_tag="WGO65_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327637.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(5041..6048)
                     /locus_tag="WGO65_001347"
     CDS             complement(5041..6048)
                     /locus_tag="WGO65_001347"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ0327638.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGKDAADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6280..6516
                     /gene="acpP"
                     /locus_tag="WGO65_001348"
     CDS             6280..6516
                     /gene="acpP"
                     /locus_tag="WGO65_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ0327639.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6672..7919
                     /gene="fabF"
                     /locus_tag="WGO65_001349"
     CDS             6672..7919
                     /gene="fabF"
                     /locus_tag="WGO65_001349"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ0327640.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         1842 a         2120 c         2069 g         1952 t
ORIGIN      
        1 tagaatgcgc tattttaatg gaaacggcgg ggttttaaac ggttcttatt tgtttgtttt
       61 gatttttgct tgggtaaaaa atcccatcat tcccacaaaa acagaagcct gaaatcccgt
      121 cattcccgcg aaagaggaat ccggtttttg ggtttcagcc attcccgata aatcgcttta
      181 gctctgccgc aaccttaaaa cggcacggta cgaaaatacc gtctgaaacc cagattgtca
      241 ggcttcagac ggcatttttt gttcaggcgg ttgccgcttc ttcgccgaac acgtcccgcc
      301 acaatttcct gacattgccg taatgtgcca acagttcgcc ggttacttcg gtttttgccg
      361 cgtcgcgcag tttggtgttg tgctgctgcc ggcggtagaa gcggtaggcg gtgcggcttt
      421 gttcggcgag gtttttgtcg ataaggctgc agtcggcggc gatattcaag agggcgatgt
      481 tgccgtagtt ggcgagaagt tgcggatatt ttcgggcatg ggcaaggatg aggtattgga
      541 cgataaattc gacgtcgacc acgccgccgc gtgcgtattt gacgttgctg tcggcaggcg
      601 gatgggtggg gaacattttt tcgcgcattt cgatgatttc gcctgacaag gcggtttggt
      661 cgcgttcggc ggtgaggatt tcggtgcgga tgcggtcgaa ggccgtctga atttcgggcg
      721 tgccgcagat gaagcgggcg cgggtaaggg attggtgttc ccacgtccag gcgttttcgc
      781 gctggtattt ttcaaaggcc gcgatgctgt gggcgaggaa accggcgtcg ccattagggc
      841 gcaggcgcag gtcggtttcg tagaggctgc ctgcgccggt ggcggcggaa agccagttgg
      901 tcaggcgacg ggcgaggcgg ctgtacacgt cgcctgcgtc ggggtgggga tcgtcgtaga
      961 gatagacgag gtcgaggtcg gaggcgtagc cgagttcttt gccgccgagt ttgccgtaac
     1021 cgacgacggc gaattgcggg gtgtcgcggt gttttttggg catgtccgcc cacgcgcaca
     1081 gcagggcggc ggcgaggatg gtgtcggcga gggcggagag ttggtcggag agggattcta
     1141 ccgtccacag tccggcgagg tcttggacgg cgagacggaa gacttgggcg tgctggaagc
     1201 ggcgcagggt gtccatttgc gcttcagtat cgccgccgca ggctttgagg tcgtctgaaa
     1261 gggcggcggc gagtgcctgc caatcaaacg cggtatccaa aagctgcgcg ctgatgagtt
     1321 cgtccaacaa aatcggatat ttgttcagat acgccgccac ccaagaactt tggcccataa
     1381 tctctgccag ttgcgccaag gtttgcggat gttcgttgag gaaggcgaga taggcggatc
     1441 ggcggctgat gttttcgaga aaatccaaca gccgcatcaa tgtatcggtc gggttgcttt
     1501 gctctgccgc cgcctgtacg aacagcggca caaccgcatc gaaacgcggc tgggcgtgtg
     1561 cggaaagatg gcggtattta tggccgtggc ggatttggtc gagccttgcg gcgacggttt
     1621 cggcatcgaa cccgtgcgcc ttcagacggc atcgccgccc ttcttcgtcg ggcttgtcct
     1681 gccatgccca ttgccattcg ctgttgtctt gcgtttgctc ttcgggttcg ctcaaaattt
     1741 cgttgaacaa ctgattgact ttgttccgat gaacattgag accgtctgaa aaagcggcat
     1801 agctgtcgaa gcccatgctt tcggcaagca gttgctgctg ttcgggcgag gtcggcaggg
     1861 tttgggtttg ctggtcgtcc cagtattgca ggcggtgttc gatgtcgcgt aggaagcggt
     1921 aggcggcaag cagggtttcg acattttcag acggcatgat gcccagctcg gcaagttttt
     1981 tcagcgtttc ctgcgtgcct ttcagttgca gcgcgcgcat ttgtccgccg cgtatcatct
     2041 ggaaaatctg ggcgataaac tcaacttcgc ggatgccgcc cgcgccgagt ttgatgttgt
     2101 ccgccatgcc ttttttgctg acttcgctgc ggatttggcg gtgtaggttc cgcatcgcct
     2161 catacgcgcc gtaatccaga tatttgcgga acacgaaggg gcgcaccagc gatttgatgt
     2221 cgttcggata cggcgtaacc acacggcctt tgcaccacgc gtagcgttcc cattctcgcc
     2281 cctgtgtaat caaatattgc tccagcgcgg tttcgctcaa taccaacgcg cccgaattac
     2341 cgtccggccg cagccgcata tcgacgcgga acacctgtcc gtcggcggtg atgtcgttca
     2401 gcagcgcaat cagtttctgc ccgactttgg tgaaaaactc ttgattgccc cgttcgcgcc
     2461 tgccgtcggt gtcgcccgat tcgggatagg tgaaaatcaa atcgatgtcg gaagacacgt
     2521 tcaactcata gccgcccgcc ttgcccatcg ccaccacgct caaatgctgc ggcgatttgg
     2581 tatcacgccc gatcggcgtg ccgtacatgt cccgataata ggcgtaggca aaatccagcg
     2641 cggtattgac ggcaaaatcg gcaaacagcg taatcgtgcg ggttacttcg ttcaaatcgc
     2701 tgatacggtt gatatcgcgc acgataatct gcgacaccac ataacggcgc aacccgcgca
     2761 actgccgcgc caactcttcc tcgttttctt ccgcacggat tgcgtcccag tcggcaaagg
     2821 cttggaaatc cgcttcggtc aacaccttgt ccaacatcgg caggaaaatt tcgggcttga
     2881 gtttgccgga atcaaggttg cgggcgagaa acagggaatg gcggcgggcg gcgtcgaggc
     2941 ggttgtcgga catttcagat tccgtttgga aggatgacgg gaagatgatg gcggattcac
     3001 ttcaaatcag gacagggcga ggggatgccg taccggttta aagtcaagcc actatatcat
     3061 agcaaccttc gatgccgtct gaaaccttac cccgacatcc agacggatgc ctgccgataa
     3121 aaaacagcgg caaatgccgc cgtttcctgt tcacacctcg ccacgctctt ttaaaacagc
     3181 ataagccgca cctttctccc gtgtggaatt tttaaaaaaa ccatcactct ttacgatttc
     3241 atgcagctcc tcacttgatt tctccgcata ttccattttc aacgccttat gctcattggc
     3301 tgcttcaatc attccctcgc ctactgcttt tgccacacca ttccaaaaac ccataatcat
     3361 gtcctccaat cgtttcaaat aaataaggtc gttgtttctg tcaagaaaca acgacagttt
     3421 ataaactgtc ggaaaacaaa atgccgtctg aaggccggat aatgcttcag acggcatcgg
     3481 attgccggtt ttaaccctgc cctccgccgt tgttcaaata gtgttcccag tgagaatata
     3541 accgggcaat attttcttca taggtgtggg cagaaggaat ataaccgtct ttgggcggca
     3601 tatacctgac aaatgattct atttcttccg gataatcaaa atctgcataa attttttcaa
     3661 cttcccccaa aggatcctca aattcttctt ttctatggaa tacatcgctt aagaggacgt
     3721 acagccaatt tttaggggaa taatccctca tttccgaagc caaatcttcc agaacaggct
     3781 ttaattcaag aatattggat ttgttgatta aagatagttt gaacacacgt tcatcatgat
     3841 ctgaaagcgt catttttcgg gcataagctg ccacatcagc ccaacccaag tatttattcc
     3901 cataccccca cagaatatcc ttccaagaaa gacggacttt ttgcgcggtt aaatcaaggt
     3961 tcatttttta ctccgattgc ctgccccgtc attccgcccc ttccgattga cgcctgttac
     4021 gaaaagtctt ccaaatgacg gctgattgcc tcttcaacag tcggatgcca atccataaat
     4081 gaagcctgca attcctctaa aaccaactca ttgaataaat catccaaatt ccgccaatcc
     4141 gttttttcat ccggatagat gaaatttgcc ataacgacag tatgtttgtt ttgggaataa
     4201 tccgataagg cgcaaatcat cagataccgc cccgaatgct ccagataaaa aattcgtttt
     4261 tccaaaggaa accggttatt ccgttgcccg ttttccattt ttcatccttt ttaacgtatc
     4321 ggcttccgcg tccttcaaat tgagttacct gccttccgcc aagcaaacgc ccgcgcccct
     4381 ttcatcattt gaatatccgt ttgaccatat ttaccagata actatcttct ttatataaat
     4441 caatcagcaa aggcacgata cccggctcat aaaataattc tgtcgtatca gatgtttctt
     4501 ttaacgcgga aattatctcc aacagttcat acccgtatcg gggataagca aacaaatagg
     4561 ggactgcttc ccgcaaacat gaaaatgctt cgtaactttg catttcccat atatgttcat
     4621 ctttaaaatc actcaaatac cattcatcca attcactttc cggatcggat atcaatcgga
     4681 taaattcatt tagttcgtcc ttaaacatat caaatcctta aatataaaat cttttgatta
     4741 tccgccgtat ccttattttc cacatcatga atttcaacgc cgttcatatt ttgcgttttc
     4801 ccaagccgtc cgccacagta taaaattatt ttttgtatca attgcttata tatttaacaa
     4861 cttaccgccg gacttgcttt ggctgcgtat caagattgtc cggttcgcgg cgggcgtttg
     4921 ctttgtttca aacagggcgg cgcgtaaggg attttcaagg ggcggcaggc ggaaaaagga
     4981 aatgaaaatg ccgtctgaac ggcaaaacgt gcgtttagac ggcattttgg gcgggacgcg
     5041 tcatcgcgcc aaagccttca aacattcttt gaccaatgcc ggacctttgt atatcaatcc
     5101 gctgtacact tggacggcgg tcgcgcccaa gcggatttta tctgccgcat ccttgccttc
     5161 cataatgccg cctacgccga taatcggcag cttgccgtcg atgtggtctg ccaacagctt
     5221 caacacccga ttactttttt catgaacggg cagcccgctc aaaccgccct gctcgcctgc
     5281 gagcggatgg ctgccgagac ttgatttgtc gatggtggta ttggtagcga tgatgccgtc
     5341 catttcgacg gatttgacaa cgtgggcgat gtcttcgatt tgtgcttcat ccaaatcggg
     5401 ggcgattttg acggcgagcg gaacgtattt cccgtataca gaggcaagct gtgcctgttt
     5461 gtttttcaaa gcctcaagca atgcgctcaa ctcgtcgcca ccttgcagcg cgcggaggtt
     5521 tttggtgttg ggcgaggaaa tattgacggt aatgtaactt gcgtgtgcgt aggctttttc
     5581 aaggcagatt aaataatcat cggcagcgtt ttcgatgggt gtaaccgcgt ttttaccgat
     5641 gttgatgccc aatacgccac tgaatttact tttttcgatg ttgcgtatca tggtgtcgat
     5701 accgtggttg ttgaaaccca tgcggttgat gatgccttgg tgttcgggaa cgcggaagag
     5761 gcgcggctgc gggttgccgg gctgcggttt gggcgttacc gtgccgattt cgatgaaacc
     5821 aaagccgagc gcgcccaatg cgtcgatgta ttcgccgttt ttgtcgagtc cggcggcaag
     5881 tccgacaggg ttgggcaaat ccatacccat caattttaca ggtttggtac ggttgccggt
     5941 tacgggaatc agacccaatt tataaaccgt gtagagcgcg tcgagcgtga agtggtgggc
     6001 tttttcggca tcgagtgcaa acaggatgcg gcgggcaagt ggatacatga cagggctttc
     6061 tgcttcagat ggaaatgccg ccattttaac cgaaatcccg ccaaacatac ggcacacagg
     6121 tcaaacttga acgacaaaac ccgccgcacc caaaaatgta caaatcggca aatattgata
     6181 taatagtcat tagatagaaa actctattaa aagtcggtac aataccaccg atttatttcc
     6241 cttaaacaac ctgccgcaag gcataaagga acgactgata tgtcaaacat cgaacaacaa
     6301 gttaaaaaaa ttgttgctga acaactgggc gtaaacgaag ccgacgtgaa aaacgaatct
     6361 tctttccaag acgacttggg cgcggattct ttggacaccg tggagctggt tatggctttg
     6421 gaagaagcat tcggctgcga aatccccgac gaagatgccg aaaaaatcac caccgtccaa
     6481 ctggctatcg actacatcaa tgcccacaac ggctaaccgg tcgtcgcccg acacaacagc
     6541 ctctgctgca ccgcgcaata gaggcttttc ccttatatgg caaactgttt gaacccctgc
     6601 cgcaaattac agacggcatc tgcacgacag ccgcatatgc ccccattccg acaaccaaca
     6661 gcgaggttat catgagtcag agaagagtag tcattacagg cttaggtcag gtttcccctg
     6721 tcggcaacac tgtcgcagag gcttgggaca ccctgctcgc tggcaaaagc ggcatcggcg
     6781 cgattacccg ctttgacgca tccgacatca acagccgtgt cgccggcgaa gtgcgcggtt
     6841 tcgacatcgg acaatacatc agcgcgaaag aagcgcgccg gatggacgta ttcatccact
     6901 acggcattgc cgccgcattg caggcaatcg ccgattcggg cttggacgat gtggaaaacc
     6961 tcgacaaaga ccgcatcggc gtgaacatcg gttccggcat cggcggactg cccagcatcg
     7021 aggtcaccgg caaagccgta atcgaaggcg gcgcgcgcaa aatcaaccct ttctttatcc
     7081 ccggttcgct gatcaacctg atttccggac acgttaccat cctcaaaggc taccgcggcc
     7141 cgagctacgg gatggtttcc gcctgtacca ccggcgcgca cgccatcggc aattccgccc
     7201 gactgatcaa atacggcgac gcggacataa tggttgccgg cggcgcggaa ggcgcaatca
     7261 gcactttggg cgtgggcggt tttgccgcga tgaaagccct ctccacccgc aacgacgacc
     7321 ccgccaccgc ttcccgtccg tgggacaaag gccgcgacgg cttcgttatc ggcgaaggcg
     7381 cgggcatatt ggtgttggaa gaattggaac acgccaaaaa acgcggcgcg aaaatctacg
     7441 ccgaaatcgt cggcttcggc atgagttccg atgcttacca tatcaccgcg ccgaacgaag
     7501 aaggccccgc ccttgccgtt acccgcgcgc tgaaagatgc cggcatcaat cccgaagacg
     7561 tggattacgt caacgcgcac ggcacgtcca cccccttggg cgatgccaac gaaaccaaag
     7621 ccctcaaacg cgcgttcggc gaacacgcct acaaaaccgt cgtcagctcg accaaatcca
     7681 tgaccggcca cctgctcggc gcggcgggcg gcgtggaggc cgtgtacagc attttggcga
     7741 tacacgacgg caaaatcccg ccgaccatca acatttttga acaagacgtt gaagccggct
     7801 gcgatttgga ctactgcgcc aacgaagcgc gcgacgcgga aatcgacgtt gccatttcca
     7861 actccttcgg cttcggcggc accaacggca cgctggtctt caaacgcttc aaaggctgat
     7921 tccgcaaagc cgccgccgac atcgaaatgc cgtctgaaac cgtttcagac ggcattttta
     7981 tag
//
LOCUS       DAWFAN010000088         7881 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWFAN010000088 DAWFAN010000000
VERSION     DAWFAN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.088"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..140)
                     /locus_tag="WGO65_001350"
     CDS             complement(<1..140)
                     /locus_tag="WGO65_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0327641.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LWT"
     gene            382..1536
                     /gene="obgE"
                     /locus_tag="WGO65_001351"
     CDS             382..1536
                     /gene="obgE"
                     /locus_tag="WGO65_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ0327642.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1619..2668
                     /locus_tag="WGO65_001352"
     CDS             1619..2668
                     /locus_tag="WGO65_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0327643.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3356..4777
                     /locus_tag="WGO65_001353"
     CDS             3356..4777
                     /locus_tag="WGO65_001353"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ0327644.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEADDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARSDKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4860..5639
                     /gene="xth"
                     /locus_tag="WGO65_001354"
     CDS             4860..5639
                     /gene="xth"
                     /locus_tag="WGO65_001354"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0327645.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5626..5970
                     /locus_tag="WGO65_001355"
     CDS             5626..5970
                     /locus_tag="WGO65_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327646.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            5981..6511
                     /locus_tag="WGO65_001356"
     CDS             5981..6511
                     /locus_tag="WGO65_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0327647.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGDVPRDWAQKQADTWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6740..7855)
                     /gene="asd"
                     /locus_tag="WGO65_001357"
     CDS             complement(6740..7855)
                     /gene="asd"
                     /locus_tag="WGO65_001357"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ0327648.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2069 a         1951 c         2037 g         1824 t
ORIGIN      
        1 gtccacagga caatggtggt cagaatgccg atgaagccgg caacggtcag ccagtgcagc
       61 gcacgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa cttattatat
      121 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      181 agaagttgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      241 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      301 cgaaattctg ttcagacggc atcaaaacac acggtaatcc gtctgaaccc cccatttgaa
      361 accaacaaac aagagcattg aatgaaattc atcgacgaag caaaaatcga agtcgccgca
      421 ggcaaaggcg gtaatggcgc aaccagtttc cgccgcgaaa aattcgtacc gcgcggcggc
      481 ccggacggcg gcgacggcgg caaaggcggt agcgtctggg cggaagccga cgaaaacacc
      541 aacaccctcg tcgaataccg cttcgttaaa cgctaccaag ccaaaaacgg cgaaaaaggc
      601 cacggttccg accgttacgg cgcaggtgcg gacgacatcg tcctcaaaat gcccgtcggc
      661 accctcatcc gcgacctcga caccgacgaa atcgttgccg acctcactta ccacggacag
      721 cgcgtctgcc tagccaaagg cggcaaaggc ggcttgggca acatccactt caaatcctcc
      781 gtcaaccgcg ccccaaaaca atccacgccc ggcgaagaag gcgaagcccg ttccctgcaa
      841 ctcgaactca aagtcctcgc cgatgtcggc ttattgggta tgcccaacgc cggcaaatcc
      901 accctgatta ccgccgtatc cgccgcacgc cccaaaatcg ccaactaccc cttcaccacc
      961 ctgcatccaa acttaggcgt ggtacgcatc gacgaaaacc acagcttcgt gatggccgac
     1021 atccccggcc tgattgaagg cgcggcagaa ggcgcgggtt tggggcatcg tttcctcaaa
     1081 cacttatcgc gcaccggatt attgctgcac gtcgtcgatt tggcgccctt cgacgaaacc
     1141 gtcaaccctg ccgaagaagc ccttgctatc atcaacgaat tgcgcaaata cgatgaagaa
     1201 ctttacggca aaccgcgctg gctggtgctg aataaactcg acatgcttga cgaagaagaa
     1261 gcccaaacgc gcactgccgc cttcctcgaa gccgtcggct gggactaccc caaacccgac
     1321 gaccgtttcc aatttgacat ggaaaccccg cgcctcttcc aaatcagcgc gctgacccac
     1381 caaggcacgc aggaattggt acaccaaatc aaccaatacc tgaccgagaa aaaacgtatc
     1441 gaagctgaaa aagccgaagc ggagaaagca gcggcaaatg ttgaaattat cgaacagcag
     1501 cctaaaacgg atacgggcgt gtttaagccg gagtgaaaaa agaaagggtt tcagacgata
     1561 tttgttctac aaaaggttgt ttgaaacaat tttaaagcaa gattgaaatg gattaaaaat
     1621 gtcatctaac caatatatcc agtcgcttga gttgacaaac tttaccgttc ttccaaacga
     1681 tacttttgaa ttttctgaaa atctcaatgt tattgttgcc gaaaacggtt gtggcaaaac
     1741 acatttgtta aaaattttat acagcctgct agaagtaaca tctaacacca aaaacagatt
     1801 actgaaaaca gaattgcaaa aaagttttgc cgataaatta ctgaatgttt tccgtcccga
     1861 ttccttgggc aggttgagca aacgtttgca ggggcgcggg cgtacggaaa tcgttttaaa
     1921 actgcaaaac ggcactacgc atagtagtct gaatttttcc agtaactcat catcgcaagt
     1981 caatgttcaa agcatcggat tgaaagaaaa tgaatatact ccgacaccta tcttcttacc
     2041 cagccgcgaa ttaattacac tttgcccttg gttcacttct ttgtatcaaa accaaagcat
     2101 ccctttcgaa gaaacttggt ttgatgcctg tatgcaatta aatcacccgc ttgccaaagg
     2161 ccccagagaa acaaaaattc gagaattgct cgaacctatt gagaacgcga tgggcggaaa
     2221 agtatctgaa gaacaaggaa ggttttatct atcgctgaca aatacgggcg gtaagattga
     2281 agcacctttg gtagcagagg gtttgcggaa atttgtcatg attgcccggt taatcgcaac
     2341 aggggcattg ctggataaag gctatctatt ttgggatgaa ccggaagcta atctgaatcc
     2401 taaattgatc aaagtggctg cccgtattat ttggtcttta tctcaacagg gtattcaggt
     2461 ttttattgca acacactctt tattcttatt gagagaattg gaacttttga agcttgaaaa
     2521 taacacggat ttacctgtac gttttttctc gttgattgct tccgatgatg gaacaaaagt
     2581 ggagcaaggg aacagtatta atgatttgaa cacattgata ttgttagatg aaaacattat
     2641 gcaggcagac aggtatctga atatttgagg ttaagctatg actacaatca cagaaaagag
     2701 attaaccttt gcttttcctg aagattatta cgttaccaag tatgacgaat gggaacatta
     2761 taaaattttt cagaacagtt gtaatttacg taataaaatt gatacaaatg aaaagggaaa
     2821 gaacgggata aatcaaagtg tagatgacga taatggcagc tcaggtgtag atataatagc
     2881 ccttcatgag tcgacacttt ggctaatcga aattaaagat tattaccgac ttgggcttga
     2941 gccaaatgcc caatcaatag atgaaaaact ttctgaccta ccatatttaa tcgcaagaaa
     3001 aattcgagat tcattggcag gattggtttc tgctaaattt aaagcagcaa aacaggaaga
     3061 aaaagatttt tctcgcttgg ctttagattg taacgaaata aaaatagtcc ttcatattga
     3121 aatgcctagt ataagaagta aactttatcc ctcgtcatca gatttagcaa acttattaaa
     3181 agataagttt aaattatcag aatttacaaa aaattttgca aactgttatg cagaaccaat
     3241 tttcacaaat atcagccata ttaataatcc gcaactgcgt aatgttcctt ggtccgtatc
     3301 tacaggaaca gaacagaaat tatcatccga gcaacaaaga ttaatccata atcccatgac
     3361 caccatctac aacaccctta cccgccaaaa agaacccttt gcccccatcg accctgaaaa
     3421 cgtgcgtatg tacgtttgcg gcatgactgt ttacgattat tgccacttag gccatgcccg
     3481 tgtgatggtt gtgttcgaca tgattgcccg ttggttgcgt gagtgcggct atccgctcac
     3541 ttatgtacgc aacatcaccg acatcgacga taaaatcatt gcccgtgcgg ctgaaaacgg
     3601 cgagaccatc ggcgagctga ctgcgcgttt cattcaggct atgcacgaag atgccgatgc
     3661 tttgggtgtg ttgcgtcccg atatcgagcc gaaggcgacg gaaaacattc cgcaaatgat
     3721 tgccatgatt gaaaccctga ttcaaaacgg caaggcatat cctgccgcaa atggcgacgt
     3781 ttactacgcc gtgcgtgagt ttgccgctta cggacaactg tcgggcaaat cgttggacga
     3841 cctgcgcgcg ggcgaacgcg tggaagtgga cggtttcaaa cgcgatccgc ttgattttgt
     3901 attgtggaaa gcggcgaaag caggcgaacc ggcgtgggaa agcccgtggg gcaacggccg
     3961 tccgggttgg cacattgaat gctctgccat gagtgaaaac ctgttcggcg ataccttcga
     4021 catccacggc ggcggcgcgg atttgcaatt cccgcatcat gaaaacgaaa tcgcccaaag
     4081 cgtcggtgcc agcggtcata cctgcggcca cgaccacgca caaacccacc acggtcaaag
     4141 cattgccagc cacgtcaaat attggctgca caacggcttt atccgcgtgg acggcgaaaa
     4201 aatgtccaaa tcgttgggca acttcttcac tatccgcgaa gtgttgaaac aatacgaccc
     4261 cgaagtcgtg cgcttcttca tcctgcgcgc ccactaccgc agcccgttga actattccga
     4321 cgcgcatttg gacgacgcaa aaggcgcgtt gacccgtcta tacaccacct tgaaaaatac
     4381 gccacccgcc gatccgatgc cgtctgaagc cgacgatgac tacacgcgcc gcttttatgt
     4441 tgcgatgaat gatgattttg atacggtgaa agcggtggcg gtgttgttcg aacttgccgg
     4501 cgaagtgaac aaaaccaatg acgcgcaact ggcaggctgc ctgaaagcct tgggcggcat
     4561 catcggactg ctgcaacgcg acccgaccga gttcctgcaa ggcggcgcgg cttcagacgg
     4621 cctctccaac gaagaaatcg aagacttgat tgcccggcgg aaacaggcgc gctccgataa
     4681 aaactgggcg gaatccgacc gcattcgcga ccttctgaac gaacacaaaa tcattttgga
     4741 agacaacgcc ggcggcacga cttggcggcg cggttgaaat ccatcgaaaa gggtaaatgt
     4801 gccgcatttt gccctttttt gttcagacgg catttttatt gtgagaactt atttttatta
     4861 tgcttaaaat catttccgcc aacgtcaacg gcatccgctc cgcctacaaa aaaggttttt
     4921 acgaatatat cgccgcatcg ggcgcggaca ttgtctgcgt gcaggaactc aaagcgcagg
     4981 aggctgattt gtctgccgat atgaaaaatc cgcacgggat gcacggccat tggcattgcg
     5041 ccgagaagcg cggttacagc ggcgtggcgg tgtacagcaa acgcaaaccc gacaatgtgc
     5101 aaatcggtat gggcattgaa gaattcgaca gggaagggcg gtttgtgcgt tgcgatttcg
     5161 acaggttgag cgttatttcg ctttacttgc ccagcggcag cagcgcggaa gaacgccagc
     5221 aggtgaaata ccgtttcctt gatgcgtttt accctatgct cgaagcgatg aaaaacgaag
     5281 gacgcgacat tgtcgtttgc ggcgactgga acatcgccca ccaaaacatc gacctgaaaa
     5341 actggaaagg caatcagaaa aattcaggtt tcctgccgga agaacgcgag tggataggca
     5401 aggttatcca caagctcggt tggacggata tgtggcgcac gctttatccc gatgtgccgg
     5461 gctacacgtg gtggagcaac cgcgggcagg cgtatgcgaa agatgtcggg tggcgcatcg
     5521 attatcagat ggttacgccc gaacttgccg ccaaagccgt gtccgcacac gtttataaag
     5581 atgaaaaatt ttccgaccat gcgccgctgg tcatggagta tgactatgct gccgaataaa
     5641 gttttgggta aatatgattg gaatgtggac ggaaaaacag gaataggcgc agcatgggtt
     5701 gtcgctgcgt ttatcctgcc catattggtg tgggcgatat ttatgttgtc gcggatgcaa
     5761 ggctggcttg cgccgaccaa ggcaaacccg acatgggcgt tggtgtggct gctgatctgc
     5821 atgccctgcc tgctgattgc gtccaaatgt ttgggttgga aaggctggcg gcgggtggtg
     5881 aatatttttg cctgcctgac cgtctgcgcc atattgagcg tacccgcttc gctgctcatc
     5941 gcctttaccc tccgggacct gctgaaatag gcggggcggt atgcagggtt ttgaactcga
     6001 agatttgacg ctttggctga tacgcgatgc cggagaggat cagatgtgga tagaccgttg
     6061 ggcgatcagt tatcccgtcg tgcagatgtc cgaagcgtcg gccggtcaaa gcatagggga
     6121 atggcaggca gggcttcgga cggcatttga acgcatacgt ggcaaatacg tcgccgttgt
     6181 cgcacacggt gcgggcgcgg ccgcattttt ggcgtggctg tatcaggcag acatcctgac
     6241 acggaagaaa attgccaaca tcatccttgt accgcagcgt cccgatattt ttcccgacga
     6301 tgcggaacac actttccaac gcgtccgctg tccctgccgt gccgcattgg tggtgtccga
     6361 acacggggat gtgccgcgag attgggcgca aaaacaggcg gatacgtgga acgcgcgcct
     6421 gttggtttcc ccgcattcgg gcagtttgaa cggtatgctc ggcggctggc agtggggtat
     6481 gaagctgatg caggaaatgt tgctggcgtg aacgcttgcc ttatcggaat ttcggctgta
     6541 gcaggaagtc cgcatatcgg gctgttttca ttgacgtgca ggcgcatcag gttcggataa
     6601 agtcgggctg ccgcataccg ccggaaatgg aatcctttat tttttacgtc ggcagtgccg
     6661 gcagggcgtt atccggcagg gttgcaagga aaatgccgtc tgaaacaggc ttcagacggc
     6721 atttccattc aaacagggct tacaggctgc ccaacacgat acgcaatacg cggcgcagcg
     6781 gctcggccgc gccccacaag agctggtcgc cgacggtgaa cgcgctgatg tattcgccgc
     6841 ccatgcccag tttgcggatg cgtccgacag ggacggacag cgtgccggta actttggcag
     6901 gagtcagctc gtggatgctg gcttcttttt cattggggat gactttcacc cagtcattcg
     6961 cgcctgccaa aatcgtttcg atttcggaaa caggcaggtc ttttttcaac ttcagagtga
     7021 tggcttggct gtggcagcgc atcgcgccga cgcggacgca caggccgtca atcacggttg
     7081 gattgtcgct gcggccgagg attttgttgg tttccacgcc gcctttccat tcttctttgg
     7141 actggccgtt gcccaaatcc acgtcaatcc acggaatcag gctgccggcg agcggtacgc
     7201 cgaagttggc tttcggatag tcttcgctgc gcaggaaatc ggatactttg cggtcgatgt
     7261 cgagaatcga gccggcagga tcggcaagtt cgtccgccac ttgggcgtga accgcgccca
     7321 taccgctgat gagttcgcgc atgtttttcg cacctgcgcc ggaagcggct tggtaggtca
     7381 tgctggttgc ccattcgacc aaatcgtttt ggaacaggcc gcccaaagcc atcagcatca
     7441 gggagacggt gcagttgccg ccgatgtagt ttttcacgcc gtttttgagg ccgttgtcga
     7501 taacgttgcg gttgacaggg tcgaggacga taatcgcgtc gtctttcatg cgcagagaag
     7561 atgccgcgtc aacccagtag ccgttccagc cgctgtcgcg cagggcttgg aagacggatt
     7621 tggtgtaatc gccgccttgg caggtaacga tgatgtccat tttggcgagt tcggcaacat
     7681 tgttggcatc taataatgtt ttagccgcct gaccgaaatc aggggctgcg ccgccgacgt
     7741 tggaagtggt aaagaaaaac gcttcaggaa tgtgggcgaa gtcgttttct tctttcatac
     7801 gctgcatcaa aaccgaaccg accataccgc gccagccgac gaaacctact ttcattgtgt
     7861 tgctccttga ggaaaaatcg t
//
LOCUS       DAWFAN010000089         7860 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWFAN010000089 DAWFAN010000000
VERSION     DAWFAN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.089"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..1334)
                     /locus_tag="WGO65_001358"
     CDS             complement(<1..1334)
                     /locus_tag="WGO65_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HEZ0327649.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELIILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDATRLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSG"
     gene            complement(1396..2745)
                     /locus_tag="WGO65_001359"
     CDS             complement(1396..2745)
                     /locus_tag="WGO65_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ0327650.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRFLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVATNREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKTNDRT"
     gene            2888..3514
                     /locus_tag="WGO65_001360"
     CDS             2888..3514
                     /locus_tag="WGO65_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ0327651.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3578..4996)
                     /locus_tag="WGO65_001361"
     CDS             complement(3578..4996)
                     /locus_tag="WGO65_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0327652.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            5323..6051
                     /locus_tag="WGO65_001362"
     CDS             5323..6051
                     /locus_tag="WGO65_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ0327653.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6110..6598)
                     /locus_tag="WGO65_001363"
     CDS             complement(6110..6598)
                     /locus_tag="WGO65_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ0327654.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFGWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(7356..7820)
                     /locus_tag="WGO65_001364"
     CDS             complement(7356..7820)
                     /locus_tag="WGO65_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0327655.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPVASESRSYVVMEEVKENPVLDLD"
BASE COUNT         2023 a         2127 c         1980 g         1730 t
ORIGIN      
        1 cccgacaggc ggatttgaag tttctccaca tatccggcgc gttcggcgcg gctggtttcg
       61 gggcggtgcg tattgagcga cgaccatttc caatgactgc gggctttgtt gagttcgggc
      121 gggagtctgg tggcgtccca cggggctttg cggctgtgca gctcgatatc cgactgtgcc
      181 gcgtgtccgc gcgtttgcag gacgtggagc aaatcgaggg cgcgggcggc gagcagggtc
      241 agggtttcag ggtcggtgtg caaggtttgg cggccggaga gtttgtcgga aatggtgcgg
      301 gtattgggca ggatgccgcc caaaaagccg ccgattgccg tacccaatcc gagcgagccg
      361 ccgagtgtgg cgatgtccag ccccaagccg atgagtgcgc cggttgccgc gcccgtgccg
      421 gtgcggatgc cgtattgttt gagcaattcg ctgtcgaacg ggtcttggcg gaaggcttgc
      481 ggcatccagt cgccgccgtc gatttcgctg tggtagaaac ggtagagggc aaacagccgc
      541 tgctgcatct gccgttcgag ttggcgtatt tccgcctgca tggtttgcag cacggtggcg
      601 gtatcctcgt tttcgtccac ttcctgcctg aaggcggcgg catcgagcag gaaatcggcg
      661 atttcgcggc gcgcttcgcc gtccagccgc tgccattcgc gccggcgcat ggctgtcagg
      721 cggtcaagtg tgctgcgttc gggcaacatg gtggcgaggt tttcccacag gcgtagttcg
      781 ccttcaaaat caaaggcgac ggtgtcgaac cctgcgaaaa cgtgcaggtt tctcctcgcc
      841 agcatggttg tccacgattc gggaggctgt ccgccggtaa agttgaacac gggcataacc
      901 ggtttggcgc accatgaaag gataatcaat tcatcccgat atttgtcgag aacgggttcg
      961 cgcgcgtcga tgacgtacat tgccatatcg ctttgcaaga cttgccgtaa gactttggct
     1021 tcctgattga aatcatggtg cgcgccgtgg ctgccgagaa actgttgcag ccgttcgatg
     1081 ccgtctgaac gattgtccgt atggttttcc agccattcca gcacgccgcc cgcgtcttcg
     1141 agtccgggcg tgtcgtacag gaaaaccagc gtgtctgcgc cgtcgctgat ggcggcttct
     1201 tcgacatgac gcgtggtcga tggggcgttt ctgacttcgc cgaaaccgct gtcgcgcaaa
     1261 agggtacgca ggagcgaggt tttgccggtg ttggtgtgtc cgacgacggc gagggaaagg
     1321 ggttgtttgt tcatgatgtt tttgaagaat ggattttcag acgacctttt aaggatggcg
     1381 gctttatggg gcgtttcaag tgcggtcgtt ggttttcaga cggccttcct gcgccgctct
     1441 gtcgggttcc agccacgccg cgccgcattc ggccagcgcg ttacgccaat gttccagctt
     1501 ttccgaaagg tcgtctgaaa gcccctgttc cgccaaaagc tgcaccaccg cgccgccctg
     1561 cgccgcttcc gaaagtcgga cgatctgccg caacacgccg cggtcgggca cagtttgggc
     1621 gcgcacgccg ataagcagtt gcgccggttt ctgcttcagc tctgtctcca gcgcggcaac
     1681 ctgttcccga ttggtggcaa cgcccttatc cagccattcc tgcgccagcc tgccctcgaa
     1741 ccattcgccg tcctgccatt cggtctccag catgaccgcc catttcggcg catcgttcaa
     1801 gacgattttc ggcgaaacgg cggacacggt ttcccgacgc gtatccgcat cggtgatttt
     1861 gttctgccaa cggcggatga ccgcctgata atagggtttt tccaaatcca atccgttttc
     1921 gcttgttttc aaaaggattt tgcataccgc ccaagccaag aagcgcggca ggatgccgta
     1981 gcaggcgata ctgccgacca gcagccccga ccaagcccgc gcatcggcaa tattgccgtt
     2041 cagacgacct tcgatgaccg cccgcgcatc aggcacggga aaacccagtt tcgcaggcag
     2101 ccatgccaac atttccacca gccgtaccga agacgaatcg cccaacagcg tgctttccca
     2161 gttgaacgta tattgccgca ccaaaagcag cagcaatacc gacaccagca ttccgagcag
     2221 cgtgcagagc cacaggctgt gcgacgttgc gcctattttc caacgtaccg aaggttgccg
     2281 ccactcgtcc gcatacagcc gcaacaccgc ctgatttaca gggtctttgc cccgaaacca
     2341 cgtcgccgga ctgctgaaaa aacgccccac tttcacgcgc aggaacaaca ttgccaacca
     2401 tactgccagc atcagcgtat tcatgcccaa cacgcccgcc aaaaccaaaa agaaattcag
     2461 accctgattg tccattagaa gataagttcc tgaaaaaccg gtaaaaaacg caaacgtcgc
     2521 cgccgccacc cacaaccaga acgaccccgc acgcacacgt tccaacgtct cccgcagcat
     2581 acggttcctg tcgatcatct ccgcccgacg gatgattttt tcctccgtac tgccgtccac
     2641 gcggcgcaaa gcctccgtcg cctgtacggg atcgccgctg aaaataaaac cgccttcgtc
     2701 caaaatacgg accagctcaa ccagttttcg ggatggattc aacataaaat gccgtctgaa
     2761 aataaaaaac agattttaac acacgcattt tcaagaatat tcacagtgta ggcaaagagt
     2821 aaatcccaca cagaggcaaa agtatcggcg taaactgact gcctctactt tcccgaaaga
     2881 ttgtgcgatg tatacaggcg aacgcttcaa tacttacagc catttgagcg gtttgattct
     2941 ggcggcggca ggtttggcgc tgatgctgct gaaaaccata ggacacgggg acggctaccg
     3001 tatcttcagc gtatcggttt acggcatcag ccttcttctg ctctatttga gttcctcgct
     3061 gtaccacgga attgcagccg gaaaactgaa aagcattttg aaaaaaaccg accactgcat
     3121 gatttatgtg ctgattgccg gaagctacac accgtttgca ctggtttctt tgagaaacgg
     3181 gccgggctgg acggtatttt cactgtcctg gctgctggcg gctgcaggaa tcgcacaaga
     3241 actcaccatt ggacggaaaa gcgaaaaacg actgctgtct attgcgattt atatcgtaat
     3301 gggctggatg gtcttggcgg taatgaaatc cctgacagcc tcactcccgc cggcaggact
     3361 ggcttggctg gcggcaggcg gtatgctgta cagcgtcggc atttactggt ttgtaaacga
     3421 tgaaaaaatc cgacacgggc acggaatctg gcatctgttc gtattgggcg gcagcatcac
     3481 ccaatttgtc agcgtgtacg gttacgtaat ctgaatgccg tctgaaaagc aaaacctccc
     3541 gatcctgaag attgggaggt tttctgtttg ccggacatca gcccttgtcg tggaactcgt
     3601 ggaattcata ctgataggac aaatcccgac ccgctttttt ctgtgccaaa taatcatcat
     3661 aaatggcacg gatttcctta cgcaacaaaa acagggctat caagttcggg ataaccataa
     3721 agccgttgaa catatcggac aggctccaaa ccaaatcgac tttgccgagc gtacctaaaa
     3781 cgatggcaag cagaaccaat gcgcgataga tgcccaagtg tcttcccctg aaaagaaaac
     3841 ggatgttgga ctcgccgaaa taataccagc cgataatggt cgtcaaggcg aaaaaggtca
     3901 gacacacggc aagcaattgc gaaccgaagc ccggaaatgc cttgttaaag gcaaattgag
     3961 taaccgccgc gccctgttcg cccgaaaggt tggcatcggt cagcaggata atcaatgccg
     4021 tagccgtaca taccaaaatc gtatcgataa acacaccgac aaatgccgcc ataccttgct
     4081 gcacaggatg cttcacatcc gcagtcgcgt gggcgtgcgg ggtagaaccc atccccgctt
     4141 cgttggaaaa cagaccgcgc gccacgccga aacgtatcgc ttcgcgcata ccgatacccg
     4201 ccgcaccgcc caaaacggct tcaggattga aggcggcggt aaagatgtgg ttgaacatcg
     4261 gcacaatata gtcggaaaat tcaaacagga taacgacggc gcacaaaata taaacaaccg
     4321 ccataaacgg cacgacaaac tgggcaatat tggcaatacg gttcacgccg ccaatcacaa
     4381 ccatgcccgc aaggacggca agcacaatac cgactgccaa agaaggcaca tcaaatgcaa
     4441 tggtaacggc agaagcaatg gagtttgcct gtgtcgcatt accgataaag cccaatgcga
     4501 taatcaacgc aatggagaaa aagcccgaca ggaaacgcgc cgcgcccctg ccgattttcg
     4561 gagtcagacc gtgcgtaata taaaacgccg gcccaccgat gtatttgccg tggctgacga
     4621 cgcggtattt ctgcgccagc agtgcctccg caaaaatcgt ggacatcccc aaaacggcag
     4681 aaacccacat ccaaaaaatc gcgcccggcc cgcctgcggt gatggcggtc gccacgccgg
     4741 cgacgttgcc cgtaccgatt tgcgcagata tggcaaccgc caacgcctga aactgcgata
     4801 aagacttgtc gtctttatcg cctttggcaa acaagccgcc gaatacagat ttgaatcccg
     4861 cacccagctt ggtaatctgc ggcgcaccga gatacagcgt aaaaaacagg ccgataccca
     4921 aaagcgcgta aatcagcagg tagtcccaaa ggaaccgatt gactgtaccc accagaacag
     4981 acaatatatt ttccataaaa taaaccttat cttacaatta aaatgactgc cttccaaaag
     5041 acattccaat aaggaaacac ggcgagcaga ccgtatttgc cgcaacagat gccttaaatt
     5101 gtcaacaatc ggggagaagc tgcgccatca taccgtaaaa tatcgttaat taaaacattt
     5161 ctttattttt aagcggaaag cggaggaaat cgctttcaga cggcatcgac aacggcacgg
     5221 cataaaacag gatattttgg gtacttgcaa cttatgttaa aatgccgacc gtaaaaaatc
     5281 tgacaaaaac agattaatta tttgaaataa gaaaggaaat ttatggcagg ccatagcaag
     5341 tgggcgaata tccagcataa aaaagcccgt caggatgcca aacgcggcaa aatcttcacc
     5401 cgtttaatca aagaaatcac cgttgcggcg cgtatgggcg gcggcgatcc cggtgcaaat
     5461 ccgcgcctgc gcctggcttt ggaaaaagca gccgaaaaca atatgcccaa agacaatgtg
     5521 caacgcgcca tcgacaaagg cacgggcaac ttggaaggcg tggaatacat cgaattgcgc
     5581 tacgaaggct acggcatcgg cggcgcggct ttgatggtgg actgcctgac cgacaacaaa
     5641 acccgcaccg ttgcggacgt acgccacgcg tttaccaaaa acggcggcaa cttgggtacc
     5701 gacggctgcg tggcgttcaa cttcgtgcat cagggctatt tggtattcga acccggcgtt
     5761 gacgaagacg cgctgatgga agcggctttg gaagccggtg cggaagacgt ggttaccaac
     5821 gacgacggtt ccatcgaagt cattaccgcg ccaaacgatt gggcgggcgt aaaatccgct
     5881 ttggaggcgg caggttacaa atccgttgac ggcgacgtta cgatgcgcgc ccaaaacgaa
     5941 accgaactct ccggcgacga tgccgtcaaa atgcaaaaac tgattgacgc gctggaagac
     6001 ttggacgacg tgcaagacgt ttacacttcc gccgtattga atttggactg atacgcagca
     6061 cagcagacat acaataaaat gccgtctgaa cctttcagac ggcattgatt tatttagccc
     6121 ttgcccacgc ccaccaaacc gtcaggacaa ccgccagaaa atacgccgcg ctccaaacag
     6181 gcaaagcaac ccccagcaga taatccggtt cggcacaatt tccgaacccg cgcacgacag
     6241 gctcgaacca accaaacaaa ggccagccct tcaatcggaa cgtccacggc gcaccgcacg
     6301 aaggagccgt acccggcggc agcgactgca accacaactg atatgccgca acagaaatac
     6361 ccgtaacggc cgggatgctg ataaagacag caccgaacaa accgcccgcc cttcttcttg
     6421 gtctgcacat caggacaatt gccgtacaca atgcggttgc caaaacgcat aaccgctgac
     6481 tgatacacaa aacgcaaggc tccataccca aaacatactg tgccgccaaa gaaccggcaa
     6541 atgcacagac cgaaacggca aacagcagcc aaacggcttt tctaaataac ggggtcattt
     6601 tctcaacaca ccaatcaaaa taccgatatg ccgattttgc tggatatatc ccgagatggc
     6661 aagggacgaa tcgacaccgg ctcaggtgcg gacgatttcg gttttcccga tatacacaag
     6721 acctaacaag ggacaaaacg gcgatttgcc cgcacaatgc ccgcaaaaaa atacatctag
     6781 aggatttgaa tttcggcaaa tttagtgaat caaaagttta tttcaatgaa atcatatgat
     6841 ttttttgaat aagcggattg acaggttttt gaaggaattt gttaccggat agccatcggg
     6901 caagtttttt gcaaaatttg aatcggtcgg gtaaattttc aaaaaataat tgacagcgga
     6961 taagaaacgg cggataattc ccgccgtcga gttgcttgat gcagcttgat ttttctcctc
     7021 tatttctcct ttgtagactt ggcacacatt caaccggatg tgtgcatttt tttatctccg
     7081 ctttttttga aatttaatta cttttattgt ttgaaattcc attctttaag aaatttaaca
     7141 agaaaaacgc ttgctttttg accggaaaag ctgcataatt cgttccgtta gctggttcga
     7201 gtagtcagtt aatagtttct cctctatttc tcctttgtag acttggcaca cattctattg
     7261 aatgtgtgca tttttttatc tgaagcaaca agcctctgtg cgtgatgttg ttatgtttca
     7321 tttaggtgtc aaaccgcata gccagcctga aatattcaat ccaaatccaa aaccggattt
     7381 tctttgacct cctccatcac aacataactc ctactctccg aagcgacagg cagttgcaat
     7441 aggatattgc ctagcatatc ccgataggca gacatatcgg gcaaacgtac tttaatcagg
     7501 tagtcgtatt cgcccgacac caagtggcat tccataattt gcggaatttt cagcacttct
     7561 tttttgaaat cttcgaaaat attgcccgat ttggattgca gcttcagctc gacaaaaacc
     7621 aatagcggtt tgcccaacag atggggattg aggtgggcgt gatagccgga aatataatgt
     7681 tcccgctcca aacggcgcac cctctctgta acgggcgtgg tggacaagcc taccttctcg
     7741 gcaagctccg tcatcgggat gcgggcattc tgttggagga tcttaagaat gcggaaatcg
     7801 attttatcta gttctttcat ttggattgcc ttgtatttat tattgatttt aacaaataga
//
LOCUS       DAWFAN010000090         7846 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWFAN010000090 DAWFAN010000000
VERSION     DAWFAN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.090"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(90..1886)
                     /locus_tag="WGO65_001365"
     CDS             complement(90..1886)
                     /locus_tag="WGO65_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ0327656.1"
                     /translation="MNTVSNYLSALREAMRAQGLDALVIPSADPHLSEYLPAHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIEHPNDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVAGKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLIDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2344..3597
                     /locus_tag="WGO65_001366"
     CDS             2344..3597
                     /locus_tag="WGO65_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0327657.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3603..3755
                     /locus_tag="WGO65_001367"
     CDS             3603..3755
                     /locus_tag="WGO65_001367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327658.1"
                     /translation="MPSETANRFQTALPRLWKKMPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            3752..5263
                     /gene="lysS"
                     /locus_tag="WGO65_001368"
     CDS             3752..5263
                     /gene="lysS"
                     /locus_tag="WGO65_001368"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ0327659.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNQGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRAVNGTANITYNGKEVDLESPFERLTILDAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDSKPGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5340..5528)
                     /locus_tag="WGO65_001369"
     CDS             complement(5340..5528)
                     /locus_tag="WGO65_001369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ0327660.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLESE"
     gene            complement(5532..6409)
                     /locus_tag="WGO65_001370"
                     /pseudo
     CDS             complement(5532..6409)
                     /locus_tag="WGO65_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            complement(6439..7566)
                     /locus_tag="WGO65_001371"
     CDS             complement(6439..7566)
                     /locus_tag="WGO65_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0327661.1"
                     /translation="MSNWKPNIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVAKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
BASE COUNT         1700 a         1993 c         2208 g         1945 t
ORIGIN      
        1 cttgcggcaa gatgaaaacg gtgcgggatg ttttgggaaa ccaatgccgt ctgaagggct
       61 ttcagacggc attttttgcg ccgtgctgtt taacgcgcca gcggttcggt gcgtttgatc
      121 agccacgctt ttgccgcgcc ttcggtcagc ggctcgaggc ggcggcggac ttcggcgtgg
      181 tagcggttga cccagtcgat ttcgccgtcg gtcatgaggg cggtgtctat caggcgggtg
      241 tcgatggggc agagggtcag ggtttcaaaa cagaggaagc tgccgaattc ggtttcttga
      301 ggggcggcga cggcttggtt ggcggcaagg ttttcaatgc ggatgcccca ttttcccggg
      361 cggtagagtc cgggttcgat ggaggtaacc atgccttttt tcatggcggt ttcgggcgtg
      421 gcgggggcgg cgaaggcgat gcgctgcggg ccttcgtgga cgttgaggaa atagcctacg
      481 ccgtgtccgg tgccgtggcc gtagtcgcat tgcgcctgcc acaggggttt gcggcaaatc
      541 gcatcaatca gcggcgaggg gatgttttct gggaacacgg cttcggcaag cgcgatatgg
      601 gctttgagaa cgagggtgtt gtcgcgtttt tgttcggcgg tgggtgtgcc gacagggacg
      661 acgcgggtga tgtcggtcgt gccgcctttg tattgcgcgc cggagtcgat gagcagcagc
      721 ccgttgccgc tgatggtgct gtggctttcg ggcgttgcgc tgtaatgcgg cagggcgccg
      781 ttggcgttga agcctgcgat ggtgtcgaaa ctcaatgaca caaaacccgg gcgcacgctg
      841 cggtggcggt aaagcatggt gtccacgtcg atttcggtca ggctgccgcc gttgccgatg
      901 atgtcttcaa actcggcgaa gaaaccgcac aacgccgcgc cgtcgtgttc catcgcttcg
      961 cggatgcggg cgatgtcggc ttcggatttg caggatttga acagcgtgga cgggttgatt
     1021 ccctcgataa ggcgcacgct ttcgggcagg cgcacaagcg tgctgacggc ggttttgttc
     1081 ggttcgataa gcaacgcgcc gccgatttgc gcgagtttgc cggcaacttg ggcgtaaggc
     1141 tcgaccgtga tgcctgcggt ttgcagcgcg gcggcggctt cggcgttcag acggcatcgg
     1201 tcggtaaaca ggacggcgtt gtctttgcca atcagcagga aggacacgaa aacgggattg
     1261 aaaggcacgt cgctgccgcg caggttggtc agccaggcga tgtcgtcaag cgaggaaacc
     1321 aagtggtaat ccgcgccttt ttccgccatc acggcgcgca cgcgggcgag tttttcggcg
     1381 gcggtttcgg agacgtaggc ggggtcgtgg atgaacacgg tttcggcagg aatggccggg
     1441 cggcttgtcc acacttggtc aagcagatcg tttgggtgtt cgatgcggat atttttggcg
     1501 gcgagtgatt gcgccaaagt gcgtttgccg gtgagcgaga ccatatcgga agggatgccg
     1561 acggcggcgt tttcgggcag gtttgccgcg agccattcgt tgtacggcgg cacttgaccg
     1621 cttttttgca gctcgatgcc gctgccggaa agctgtttgg cggcttgttc ccaatagcgg
     1681 ctgtccaccc atacacccgc ttcatcggtg gtcaggacaa atgtgccgac cgagccggta
     1741 aagcccgaca attcgcggcg cgcctgccag tgtgcgggca ggtattcgga caggtggggg
     1801 tcggcggaag ggatgacgag tgcgtccaag ccttgcgccc tcatggcttc gcgcaatgcg
     1861 gacaggtaat tcgatacggt attcataaca gttcctccaa tcgggttttg cggcttcaga
     1921 cggcatggac ggaaatctga aatgccgtct gaaaaacata accatcttat caaatcgcca
     1981 gcctgctgca aaacggacgg gctgatcgga tggtgcgtgt ttgcgcgaaa aaagcgatgt
     2041 aaggttgttt gttgtaaaat tttaggcata aaatgtcgtt tcatgccttt ttttggaagt
     2101 aatatggaaa atatagtcaa gtttgagcat gaagttctaa tagtgtgtgt tggcaggtat
     2161 ttggattacg ttacaatacg cgctttcatc aaaatttccc taccgagcgg tatcctgttt
     2221 ttgaagacgg taggatttgg gtttgtttgg gggcaatccc gcccaaattg gagcaataga
     2281 tagggctgtg tccgaatatg cggctctgtg tgttgaaaca cggttaaaaa tacggaagac
     2341 tttatgtccg aacaacatat ttcgacttgg aaaagtaaaa tcaacgcatt gggaccgggg
     2401 attatgatgg cttcggcggc ggtcggcggt tcgcacctga ttgcctcgac gcaggcgggc
     2461 gcgctttacg gctggcagat cgcgctcatc atcatcctga ccaacctctt caaatacccg
     2521 tttttccgct tcagcgcgca ttacacgctg gacacgggca agagcctgat tgaaggttat
     2581 gccgagaaaa gccgcgttta tttgtgggta ttcctgattt tgtgcatcct ctccgccacg
     2641 attaacgcgg gcgcggtcgc cattgtaacc gccgccatcg tcaaaatggc gattccctcg
     2701 ctgatgtttg atgccggcac ggttgccgcc ttgattatgg catcctgcct gattattttg
     2761 gtgagcggac gttaccgcgc tttggatcgc gtttccaaaa tcatcatcgt tactttgagt
     2821 atcgccacgc ttgccgccgc cggcatcgct atgtcgcgcg gtatgcagat gcagcccgat
     2881 tttatcgagc cgacaccgtg gacgcttgcc ggtttgggct tcctgatcgc gctgatgggc
     2941 tggatgcccg cgccgattga aatttccgcc atcaattctt tgtgggtaac cgaaaaacaa
     3001 cgcatcaatc cttccgaata ccgcgacggg atttttgatt tcaacgtcgg ttatatcgcc
     3061 agtgcggttt tggctttggt tttccttgca ctgggtgcgt ttgtgcaata cggcaacggc
     3121 gaagcagtac agatggcggg cggcaaatat atcgggcaat tgatcaatat gtacgccgtt
     3181 accatcggcg gctggtcgcg cccgctggtg gcgtttatcg cgtttgcctg tatgtacggc
     3241 acgacgatta ccgtcgtgga cggctatgcc cgtgccattg ccgaacccgt gcgcctgctg
     3301 cgcggaaaag acaaaacggg caacgccgaa ttctttgcct ggaatatttg ggtggcgggc
     3361 agcggtttgg cggtgatttt ttggtttgac ggcgtaatgg cgaatctgct caaatttgcg
     3421 atgattgccg cttttgtgtc cgctcctgtg tttgcctggc tgaattaccg tttggtcaaa
     3481 ggtgatgaaa aacacaaact cacatcaggt atgaatgccc ttgcattggc aggcttgatt
     3541 tatctgaccg gttttaccgt tttgttctta ttgaatttgg cgggaatgtt caaatgataa
     3601 cgatgccgtc tgaaaccgca aaccgctttc agacggcatt gccgcgttta tggaaaaaaa
     3661 tgccgacatc gcgtaaaata cgcgcaaatt ttgtgattcg gtcaggtcgt ctgaaacaga
     3721 ttgcgcctga tttatttttt cggaaaaccc tatgagcgaa caaaaccatc cgcaaaccga
     3781 gccgcagttg gacgaaaacc aaatcatcgc cctgcgccgc gaaaaactgc acaacatccg
     3841 ccaacagcgc aacgcctatc ccaacgactt caaacgcgac agcttcgccg ccgatttgca
     3901 cgcccaatac ggcgaaatcg gcaaagaaga actcgatccg caaggcattc ccgtcaaagt
     3961 ggccggccgc atgatgctga agcgtcaaat gggcaaggcg agctttgcca ctattcaaga
     4021 cgtgtccggg caaatccagc tttatctgaa caacaaaggc gtgagccaag aagttttgga
     4081 cgacttcaac cattgggatt tgggcgacat cgtcggcgcg gaaggcactt tgttcaaaac
     4141 caaccagggc gaactgaccg tacgcgtgtc cggcatccgc ctgctgtcca aatccctgcg
     4201 ccctctgccg gacaaacata aaggcttgag cgatcaggaa accaaatacc gccaacgcta
     4261 tgtcgatttg attgccaacg aagaatcgcg caataccttt atcaaacgca gccaaatcat
     4321 ccaatccgtg cgtaatttta tggtgggcga gcattatctc gaagtcgaaa ccccgatgat
     4381 gcacccgatt cccggcggcg cgacggcaaa acccttcgtt acccaccaca atgccttgga
     4441 cattccgctc tacctgcgca tcgcccccga gctgtatctg aaacgcttgg ttgtcggcgg
     4501 tttggaacgc gtgtttgaaa tcaaccgcag cttccgcaac gaaggtatgt ctgtgcgcca
     4561 taaccctgaa ttcaccatga tcgaattcta cgaagccttc tccgactacg aacgcatgat
     4621 gcaaatggcg gaagacatca tccgcaacgc ttcccgcgcg gtaaacggca cggcaaacat
     4681 cacttacaac ggcaaagaag tcgatttgga aagcccgttc gagcgtctga ccattctcga
     4741 tgccatcaaa aaatacaatc cgcactacac cgacgagcag ttgaacgatg cggagtggct
     4801 gaaaaaagaa atcgtcaaac acggcgaaag cctgcctccg tctccgggca tcggcagcct
     4861 gcaacttgcc ctgtttgaag gttgcgccga gggcaaattg tggaacccga ctttcattat
     4921 cgactaccct gtcgaagtgt ccccgttggc acgcgcatcc gacagcaaac cgggcttgac
     4981 cgaacgcttc gagcttttcg ttgtcggccg cgaactggca aacggctact ccgagttgaa
     5041 cgatccggaa gaccaagccg aacgcttcaa ggcacaagtg gcgcaaaaag acgcgggcga
     5101 cgacgaagcg atgcactacg atgccgacta catccgcgcg atggaattcg gcctgccgcc
     5161 gaccggcggc tgcggcatcg gcatcgaccg cttggtcatg ttgctgaccg attcgcaaac
     5221 catccgcgac gtgattctgt ttccgcagat gcgccccgag tagtcataaa aacagttgaa
     5281 atggcaatgc cgtctgaacc cgattggatt cagacggcat tttgtatggc ggtacggatt
     5341 tattcgcttt ctaactgacc gacccattcg gacaaggcag tcaggcgttg ttcggatttc
     5401 gccacgaacg ggttttcggt atcccacgcg tagcctgcca aaatcgaaga aagcatacgg
     5461 ctgatttccg ggctgcggtc gggcgcgtac acgacgtttt ggaagcggaa atcgtaccaa
     5521 ccgtccacac atcaggggtg gtaatccgaa tcaacacgcc ttcttgcctg tgcggcggac
     5581 ataaaccgtt ttttgactcc agcaccacac ctaaaatcgt gctgccttcg ggcaggtcaa
     5641 aggcttcaga cggcctgccc gtctgataaa tcagcatcct gcccgcaata tcgataacaa
     5701 ggtgcgcgac gaccagccgc ttgtcggaca gttcaatcag cacgatgtcc tcgtccgcag
     5761 ggcgcatacg cggctcgacc accaccaaat cccctttttc gataaccggc gacatggacg
     5821 tgtcgggcat ggcaacggca aacgtgtact gcgagggggc gaccaccgaa gcaaacaagt
     5881 cgaaactgcc tgtgagtttg cctgtacggg cgaaagccgc cgcctgacgg tctgacaaca
     5941 gggggcgtgg tgtgagcctc atcgacagtg ctttgccgtc ggcatcgctg tggttgtgcg
     6001 tatgcgtatg atggttgttg atggtcgtat cgccttggac atcgttaagg actatgctgt
     6061 ttgaacctgc cacggatttg gtcatctcat tgtaggcttg cgtcgtgatg cgctcttgct
     6121 tggtcatacc tgttacagcc tgtttcagca tttctaacgc tatgtgattc ttcctaaggg
     6181 tcgctatacc ggcttttgca gcttgctcat taccacaaga ggggcaaccc cagctacttc
     6241 ctatgaacat attggagtac ctcgaacagg tttggtttcc atgtttaggg cagttgatta
     6301 cacacggctt ggctgtaccc tcaaactcga ccaacttaat gccacgatga ccgaaacgtt
     6361 cctcaacctc agcagcagct tcttcaaatg tttttgacct tcttgccata ttgacctccg
     6421 gattattgat tattttggct atgctttaac gagggattgg aatgaggtaa agctgttgcc
     6481 ctctccccgc aatagttcca ttaggcgcgg atgaatgaat agtttgtccc tgccgatggc
     6541 gatttcttgc agcacaccta tgtctgaaag ctctttcagg tacttagagg ccgtctgccg
     6601 tttggctatc cctgccgctt ctaggttggc aatgcgtgta tatggctgct caaacagaag
     6661 atttaccagt tcgtgcgtgt agattccttg tgcgtgtgtc cgtatgtgtt gacgtgtctg
     6721 ctcaaacagg cggcgtatcg catctatttt cgctaccgtc caatcggcgg tgtcggctac
     6781 gccgtctaag atgtagatta tccagctttc ccagtcctgc cgttcggtta cgcctaaaag
     6841 caggcggtaa tagtccgccc tgttttcgat aatatagcgg ctcaaataca aaataggcaa
     6901 atccaaaagc cctttttcaa tcaatagcag gctgttcaat atgcgccccg tccgcccgtt
     6961 gccgtccgta aacggatgga tggcttcaaa ttggtaatgt gccgccgcca tgatgataag
     7021 cgggtctaaa tcgccgcttt cgtgaataaa ccgctcccaa tttgccagct tgccgcgtat
     7081 ggtttcttct ccttcgggcg gggtatagac aacatttccg ctgttgcctc cttttagggc
     7141 tgtgccgcct gttttgcgga tggccatttc gtaggggtgc ttgatggcgt tgcagaccat
     7201 gatggcggtt tgtgtgcata aagggcggct cgtcagtgat tcatagcctg caaacagggc
     7261 ggtgcggtat tgcagggctt ctttcgtggc agggtcttgc cgttccgtat ccatttgcag
     7321 ggattgaaac agcttgtccg tggtggttac gatgttttca atttccgaac ttgcacgggc
     7381 ttccataaca ggaagggtgt taatcagcat ggcttgattc ggtatcaatt ctgccgcctg
     7441 ctttaaacgg gcaagggatg cacgggcggc tatacaacgt ttcaggatgg ttttgctttc
     7501 aatatcctgt tttggcggca ggggtggtaa atcgttatag ggaatattgg gtttccagtt
     7561 gctcatattt aaaatttcgg aaaatttaaa gatgtttcca gtatatgttt acgccgtgta
     7621 tatatcaagg atatatgttt aaaaatttgg cttttgtaaa tatatgggcg gtaaaaccgc
     7681 attattttat gttgttacct attttacacc ttgtctgcac acgatttcca aacaacctaa
     7741 aaagaaacct gccgaaaggc aggtttaaag aggtcatttt aaatgtgaac taaagcttac
     7801 accctcgtag ggtatcgtgt atatcagacg tacttaatat acacta
//
LOCUS       DAWFAN010000091         7829 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWFAN010000091 DAWFAN010000000
VERSION     DAWFAN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.091"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(198..614)
                     /gene="dksA"
                     /locus_tag="WGO65_001372"
     CDS             complement(198..614)
                     /gene="dksA"
                     /locus_tag="WGO65_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ0327662.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(758..1549)
                     /locus_tag="WGO65_001373"
     CDS             complement(758..1549)
                     /locus_tag="WGO65_001373"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ0327663.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTLSRYIGG
                     TRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTIWLEDEAQMHGI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAINEGVCACVRRSQEMERQYQ"
     gene            complement(1598..2005)
                     /locus_tag="WGO65_001374"
     CDS             complement(1598..2005)
                     /locus_tag="WGO65_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ0327664.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCQLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQGGFDCLEKQGLRRNGLSERVRW"
     gene            complement(2005..2691)
                     /locus_tag="WGO65_001375"
     CDS             complement(2005..2691)
                     /locus_tag="WGO65_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ0327665.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRKPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2811..3854
                     /locus_tag="WGO65_001376"
     CDS             2811..3854
                     /locus_tag="WGO65_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ0327666.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            4017..5243
                     /locus_tag="WGO65_001377"
     CDS             4017..5243
                     /locus_tag="WGO65_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0327667.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5529..7679
                     /gene="yccS"
                     /locus_tag="WGO65_001378"
     CDS             5529..7679
                     /gene="yccS"
                     /locus_tag="WGO65_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ0327668.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1915 a         2592 c         1712 g         1610 t
ORIGIN      
        1 caggggaaaa cggtcaatgc tttcagtggg attttttttg gggaaattcg tcatgtcgct
       61 gtcggataag gttttttatt tctgctaaat gctgcgccgc ctccaacaat cccttcctct
      121 ccctcctctg accggtgcgc ctttgtgaat atgccgtctg aaactcgggg actcagacgg
      181 cattttgttt gccgccatca gtcggcaaac tgttttttca tcctctctcg gcgttcttgg
      241 gactcaacag ataaagtggc tgtcgggcgt gccagcagcc gcttcagacc gataggctct
      301 cccgtatcgg cacagaatcc ataatcccct tcatcaatat tgcggatggt cgcctgtatt
      361 ttactgagaa gttttcgttc ccgatcgcgg gtacggagtt ccaatgcgta ctcttcttcc
      421 tgtgtggcac ggtcggcagg atcgggggct gattcgtgtt cttggaggtg ccctgtcgta
      481 acagatgcat tctctatgag ttcatcttgc attttaacca gcaattcacg aaaaaaagcc
      541 aaatggtcgt cattcatata atcgtcttcc ggcccattcc aattcaaaat atcttgttct
      601 gtcagctttg ccataatgta tgtcctctca gttcaccgaa aaaaaagcac cacaaagtgg
      661 ctttattcct ttgattttta taaggcttat tgcctaaggc gtttttgaaa acgcgcatca
      721 taacacgttt tggattggtt tttttatttt ctctacatta ttgatattgc cgttccattt
      781 cctgcgaacg gcgcacacag gcacaaacgc cttcatttat ggcttcggcg acacgatgcc
      841 gtctgaaggc ttccacggct tcgtgagttg tcccgccttt tgacgttaca tttttttgaa
      901 gcttctcgaa atcttcacca gtctgctcgg caagggcaac cgctccttta aatgttgcca
      961 gactgagcgc gcgtgcttct gccatatcaa acccttgtcg gatggcggca ttttgcaatg
     1021 cgtccagcag ataaaacaca taagccggcc cgctgccgct gatgccggta atgccgtgca
     1081 tttgcgcttc atcttccaac caaatggtca aaccgactga tttcatgatt cgatcggcaa
     1141 tcctgcggtc tgtttccgat acttccgctt cggcaaacat accggatacg cccagcccga
     1201 tttttccggg tgtattcggc ataacccgga caatgcggcg tgttccccca atgtaacggc
     1261 tgagcgtatc gaccggcaat ccggctgcga cagaaagcac caatgcgccg ttggtgcgga
     1321 tatttttgca cgcggcttcc atatcctgcg gcttgacggc aaggattaaa acatcgtcgg
     1381 aatgaagctc cggcaaggtt tcagaagttt cgacccccaa ctctttttgc aaacgttcgc
     1441 gcttttccgc accccggttg gcaatatgga tgcggtaacc gccttgtttg accaatccgc
     1501 ccgcaacggc agccgccata ttgccgccgc cgagaaaata aacattcatt atttctaccc
     1561 tattcaaaat taccttacta aattcattcc caatttttta ccatcggacg cgctcggaca
     1621 gaccgttgcg ccgcaacccc tgcttttcca agcagtcaaa tcctccctgt ttgaacgtct
     1681 caatcaaagg tttgccgtca cgcgtacgcc aatccagccc gcaggcttca aacttcccta
     1741 ttttcctgta aaagtaagat tgttgtgcgg attcatattc gtttgccctg acggaacgat
     1801 tgccgtctga aagcggtatc tcgtagtcct ctaaagacgg cggactctcg ccgccaatat
     1861 cccaaaaatc gatggcagcc ggttttctcg gcttacacca gccggacagg gacgaacact
     1921 cataccaacc cgccaactgg cagcctgcaa ccgtaaatgc aagcaacgca gccattgccg
     1981 tatattttat tgcccgaatt tccatcagcc ccttttcccg aaaatcgcgc tgccgatacg
     2041 gacgtgtgtc gcaccgcact caatggcggc aggcatatcg tccgacatcc ccatagacag
     2101 cacgtctgcc ttaacgccag ccgcattgag gtcggcaagc agtttccgca tcgtttgaaa
     2161 ttgcaccttc aactccgttt cactgctgtt ggctttggca acacacatca gtccgcgtac
     2221 gacgatattc ggcagcttcg ccacttccac agcaagcgcg actgcttctt cgggcgcgac
     2281 accgtgcttc accgcctcgc ccgcaatgtt cacctcgata cacacctgca aaggcggcat
     2341 tgaggaagga cgttgcccgc tcagccggac agcggttttc agacggcata cggtatgcac
     2401 ccaatgcgca cgttcggcga caaacttggt tttgttggac tgcacatcgc cgatgacgtg
     2461 ccacacgatg tcagtcaaat ccgccaactc ttccgtttta ccgtaccact cctgaatata
     2521 gttctcgccg aaatcacgct gtccggcggc gtaaacttcg cggatgccgt ctgaagggaa
     2581 agtcttactg acggcaatca ggctgacgga atgcggcttc cttcccgcct gcagaaccaa
     2641 ttttccgata cggtcggaca cctcacaata acgttcttgc aacaccgtca tagattatcc
     2701 cctaattaaa aatgattaaa cagttgaaac ccctccagtc agggcggtac aatcaaggtt
     2761 gttagaacca ttccaaccaa tcgaaacatt atactaaaca gagccgcatt atgcagatta
     2821 ccgacttact cgccttcggc gctaaaaaca aagcatccga ccttcacctg agttcgggca
     2881 tatcccctat gattcgggtt cacggcgata tgcggcgcat caaccttccc gaaatgagcg
     2941 cggaagaggt cggtaatatg gtaacttcgg tgatgaacga ccaccagcgg aaaatctacc
     3001 agcaaaactt ggaagtcgac ttctcgttcg aactgcccaa cgtcgcccga ttccgcgtca
     3061 acgccttcaa caccggccgc ggtcccgccg ccgtattccg caccattccc agcaccgtct
     3121 tatcgctgga agaattgaaa gccccgagca ttttccaaaa aatcgcagaa tcgccgcgcg
     3181 gcatggtatt ggttaccggc cctaccggtt cgggcaaatc gaccacgctt gccgcgatga
     3241 tcaactacat caacgaaacc cagccggcac acatcctgac catcgaagac ccgatcgaat
     3301 tcgtccacca aagcaaaaaa tccctgatta accagcgcga gctgcaccag cacaccctca
     3361 gcttcgccaa cgcgctgcgt tccgcattgc gcgaagaccc cgacgttatc cttgtcggcg
     3421 agatgcgcga ccccgaaacc atcggcttgg cactgaccgc cgccgaaacc ggacacttgg
     3481 ttttcggcac gctgcacacg accggcgcag caaaaaccgt cgaccgtatc gtggacgtat
     3541 tcccggcggg agaaaaagaa atggtgcgct ctatgctgtc cgaatcgctg accgccgtca
     3601 tctcccaaaa cctgctgaaa acgcacgacg gcaacggccg tgtcgcctcg cacgaaatcc
     3661 tgattgccaa ccccgccgtc cgcaacctca tccgcgaaaa caaaatcacg cagattaact
     3721 ccgtcctgca aaccgggcag gcgagcggta tgcagacaat ggaccaatcg ctgcaatcgc
     3781 tggtgcgcca agggctgatc gcaccggaag tcgcacgcag acgcgcgcaa aacagcgaaa
     3841 gtatgagttt ctgacacaca accgccttcc ggccataccg gcgggaaaac aaggcgcaaa
     3901 cacgcggggc gggacgcagc atcccgcccg gctacctttc cgaacaagaa gcgtccgcct
     3961 tcctgttgaa acctgccgcc gcaaactgca aggcttaaac cgaaaagaag ctaacgatga
     4021 ataccgataa cctgcacgac atcttggacg aaatggttca agtgtattct caaaaaaaac
     4081 aaagccgatc cgaaaccccg gccgaaatcg gcgcacactt ccacccgctg ctcgaccgcc
     4141 tgtgcgaaac cgcagaagca caaaacgcgt ccgacatcct tatcagcaaa ggattcccgc
     4201 cctcgttgaa aatcaacagc gcattaaccc cgcagccgca aaaggcgctg acgggcgagg
     4261 aaaccgccgc catcgccgca tcgacgatga acgccgaaca atcggaaata ttccggcgcg
     4321 acggcgaaat caactattcc gtccagtcgc gcagcggcac gcgctaccgc gccaacgcct
     4381 accacagcca aggcagcgca ggtttggttt tgcggcgcat caaccacgtc atcccgcaaa
     4441 tgcaggaatt gggcctgccc gaaaaactca aagacctcgc cgtcgcgccg cgcgggctgc
     4501 tgattatcgt cggacctacc ggttcgggca aatccaccac gatggcgact atgctcgaac
     4561 accgcaacaa aactctgccc ggccatatcg ttaccatcga agacccgata gagtttatct
     4621 acaagccgcg ccgctgcatc tttacccagc gcgaaatcgg cgtcgacacc ataaactggc
     4681 agacggcggt acaaaacgct atgcgccagt cccccgacgt ggtctgcatc ggcgaagtcc
     4741 gcagcaggga aagtatggaa tacgcgatgc agctcgccca aaccggccac ctgtgcattt
     4801 ttacgctcca cgccaacacc gcgccgcagt cgctcgaacg catactcaac ttctacccca
     4861 aagaacagca caaccaaata ctgatcgaca tcgccctcaa cctgaccggc atcatctgcc
     4921 aacgcctcgc cctcaaacaa gacaaaacgg gcaggacggc ggttgtcgac ttgctcatca
     4981 acacgcccgc catccaagac ttcatcctga agggcgacct gatgaacatc agtaaaatca
     5041 tggaaaccgc caaaaccgac ggaatgcaga cgatggatca aaaccttttc gaactgtacc
     5101 gtcacggcat catcagttac gaagaagccc tgcgccagtc cgtttccgcc aacaacctgc
     5161 gattgcacat ccaactgcac aaagaaggca aaacgcccga actcctttac gacagggtca
     5221 acggtctcaa cctcatttcc tgatccgcaa aacccaatgc cgtctgaaaa ccgcatcccc
     5281 gttttcagac ggcatgattt tatccgcccc attcatgtgc tacactttat agtggattaa
     5341 atttatagtg gattaacaaa aatcaggaca agacgacgaa gccgcagaca gcacaaatag
     5401 tacggaaccg attcacttgg tgcttgagca ccttagagaa tcgttctctt tcagctaagg
     5461 cgaggcaacg ccgtactggt ttttgttaat ccactatatt cacttcataa caataaaccg
     5521 gtaaaaccat gaaaacccca ctcctcaagc ctctgctcat tacctcgctt cccgttttcg
     5581 ccagtgtttt taccgccgcc tccatcgtct ggcagctagg cgaacccaag ctcgccatgc
     5641 ccttcgtact cggcatcatc gccggcggcc ttgtcgattt ggacaaccgc ctgaccggac
     5701 ggctgaaaaa catcatcgcc accgtcgccc tgtttaccct ctcctcgctc acggcacaaa
     5761 gcaccctcgg cacagggctg cccttcatcc tcgccatgac cctgatgacc ttcggcttta
     5821 ccattttagg cgcggtcggg ctgaaatacc gcaccttcgc cttcggcgca ctcgccgtcg
     5881 ccacctacac cacacttacc tacacccccg aaacctactg gctgaccaac cccttcatga
     5941 ttttatgcgg caccgtactg tacagcaccg ccatcatcct gttccaaatc atcctgcccc
     6001 accgccccgt ccaagaaagc gtcgccaacg cctacgacgc actcggcggc tacctcgaag
     6061 ctaaagccga ctttttcgat cccgacgaag ccgaatggat aggcaaccgc cacatcgacc
     6121 tcgccatgag caacaccggc gtcatcaccg ccttcaacca atgccgttcc gccctgtttt
     6181 accgccttcg cggcaaacac cgccacccgc gcaccgccaa aatgctgcgt tactactttg
     6241 ccgcccaaga catacacgaa cgcatcagct ccgcccacgt cgattatcag gaaatgtccg
     6301 aaaaattcaa aaacaccgac atcatcttcc gcatccaccg cctgctcgaa atgcagggac
     6361 aagcctgccg caacaccgcc caagccctgc gcgcaagcaa agactacgtt tacagcaaac
     6421 gcctcggccg cgccatcgaa ggctgccgcc aatcgctgcg cctcctttca gacagcaacg
     6481 acagtcccga catccgccac ctgcgccgcc ttctcgacaa cctcggcagc gtcgaccagc
     6541 agttccgcca actccagcac aacggcctgc aggcagaaaa cgaccgcatg ggcgacaccc
     6601 gcatcgccgc cctcgaaacc ggcagcctca aaaacacctg gcaggcaatc cgtcctcagc
     6661 taaacctcga atcaggcgta ttccgccatg ccgtccgcct gtccctcgtc gttgccgccg
     6721 cctgcaccat cgtcgaagcc ctcaacctca acctcggcta ctggatacta ctgaccgccc
     6781 ttttcgtctg ccaacccaac tacaccgcca ccaaaagccg cgtccgccag cgcatcgccg
     6841 gcaccgtact cggcgtaatc gtcggctcgc tcgtccccta ctttaccccc tccgtcgaaa
     6901 ccaagctttg gatcgtcatc gccagtacca ccctcttttt catgacccgc acctacaaat
     6961 acagcttctc gacatttttc atcaccattc aagccctgac cagcctctcc ctcgcagggt
     7021 tggacgtata cgccgccatg cccgtacgca tcatcgacac cattatcggc gcatcccttg
     7081 cctgggcggc ggtcagctac ctatggcccg actggaaata cctcacgctc gaacgcaccg
     7141 ccgcccttgc cgtatgcagc aacggcgcct atctcgaaaa aatcaccgaa cgcctcaaaa
     7201 gcggcgaaac cggcgacgac gtcgaatacc gcgccacccg ccgccgcgcc cacgaacaca
     7261 ccgccgccct cagcagcacc ctttccgaca tgagcagcga acccgcaaaa ttcgccgaca
     7321 gcctgcaacc cggctttacc ctgctcaaaa ccggctacgc cctgaccggc tacatctccg
     7381 ccctcggcgc ataccgcagc gaaatgcacg aagaatgcag ccccgacttt accgcacagt
     7441 tccacctcgc cgccgaacac accgcccaca tcttccaaca cctgcccgaa accgaacccg
     7501 acgactttca gacagcactg gatacactgc gcggcgaact cgacaccctc cgcacccaca
     7561 gcagcggaac acaaagccac atcctcctcc aacagctcca actcatcgcc cggcagctcg
     7621 aaccctacta ccgcgcctac cgccaaattc cgcacaggca gccccaaaat gcagcctgaa
     7681 aaagtttcgg cattttgtaa gagagggcag attgtcagac aggttgcaag atagtggata
     7741 aggctttgcg cagggtaaat gcgtagcaac tgaaccgtca ttcccacgaa cctacatccc
     7801 gtcattccca cgaaaacaga aaaccaaaa
//
LOCUS       DAWFAN010000092         7594 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWFAN010000092 DAWFAN010000000
VERSION     DAWFAN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.092"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WGO65_001379"
     CDS             complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WGO65_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ0327669.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RTATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1552..1707
                     /locus_tag="WGO65_001380"
     CDS             1552..1707
                     /locus_tag="WGO65_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327670.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1704..2135
                     /locus_tag="WGO65_001381"
     CDS             1704..2135
                     /locus_tag="WGO65_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ0327671.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2269..6618
                     /gene="app"
                     /locus_tag="WGO65_001382"
     CDS             2269..6618
                     /gene="app"
                     /locus_tag="WGO65_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ0327672.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFSYQIVKRNNYKPDNSHPYNGDYHM
                     PRLHKFVTDAEPVEMTSDMRGNTYSDKEKYPERVRIGSGHHYWRYDDDKHGDLSYSGA
                     WLIGGNTHMQGWGNNGVVSLSGDVRHANDYGPMPIAGAAGDSGSPMFIYDKTNNKWLL
                     NGVLQTGYPYSGRENGFQLIRKDWFYDDIYRGDTHTVFFEPRSNGHFSFTSNNNGTGT
                     VTETNEKVSNPKLKVQTVRLFDESLNETDKEPVYAAGGVNQYRPRLNNGENLSFIDYG
                     NGKLILSNNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRL
                     SKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVSGRGTVQLNA
                     DNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNATTTSTVTITGNESI
                     TQPSGKNINRLNYSKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNG
                     NITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWINRTFKAENFHIQG
                     GQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKV
                     IASLTKTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGNLSLVGN
                     AQATFNQATLNGNTSASGNASFNLSNNAAQNGSLTLSDNAKANVSHSALNGNVSLADK
                     AVFHFENSRFTGQLSGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAA
                     GAQTGSVSDTPRRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFRFMSELFGYRS
                     DKLKLAESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGA
                     WRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAGRDAAEKT
                     ESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARR
                     DLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVW
                     TSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNS
                     ARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGGKIRRRVLHYGIQARYRAGFG
                     GFGIEPYIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPY
                     LSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLE
                     AQHSAGIKLGYRW"
     gene            complement(6934..7095)
                     /locus_tag="WGO65_001383"
                     /pseudo
     CDS             complement(6934..7095)
                     /locus_tag="WGO65_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1946 a         2192 c         1868 g         1588 t
ORIGIN      
        1 aaacggatcg gattcccgtt tttatgggat aacggaatgt tcagccggac accgccacga
       61 tgctttcgat ccgtttattt tccgatgcag aatctcgaaa aaatcacgcc gagcaggtcg
      121 tccgccgtaa actcgcccgt gatttcgccg catgcgacct gagccaagcg caagtgttcg
      181 gcaaacagct cgatttgatg gttgccgcac aatgccgcca gcgacaattc ttcctgcgct
      241 gctttgagtg cgttgacgtg ccgcgtccgc gccaaaaaca gcccttcgct ttcgccctgc
      301 caaccggcct cacgcaacaa cgtccgtttc agcgcgtcca agccgtcgcc agttttcgcc
      361 gacagcgcga tgacggtttc cgcgcccgta ccgaacccgc ctgccgcgtg tgcgtgcaaa
      421 tcggatttgc tgtggatttc gatgcgtttc aactccggcg gcaacgcgtc caaaatcgcc
      481 cgtgtctttt cattcaaacc ctcgcgcgga tcgaccaaca ccagcgcgac atcggcttcg
      541 gatacggctt tgcggctgcg ttcgatgccg atgcgttcga ccacgtcgtc cgtctcgcgc
      601 aaacctgccg tatcgacaat atgcaccggc acgccgtcaa tcaggatacg ttccctgacc
      661 gcgtcgcgcg tcgttccggc aatatcggta acaatcgcca cttcgtcgcc cgccagcgcg
      721 ttcaacaggc tggatttgcc gacattcggc gcgccgacca atacgacatt cagaccttcg
      781 cgcaaaatcg cgccctgctg cgcgttggca agcacatcat ccacggcgcg gcgcaagccg
      841 tccagtttgc cgcgtgcgtc tgccgcttcg agaaaatcaa tgtcttcctc gggaaaatcc
      901 agcgtcgctt cgaccagcat ccgcaaggta atcaaatctt cgaccagacc gtgtatccgc
      961 cgcgaaaaat cgcccttgag cgagcgcaac gccagacgcg ccgccgaacg gctggatgcg
     1021 tcaatcaaat ccgccacgcc ttccgcctgt gccaagtcca gtttgtcgtt caaaaacgca
     1081 cgtttggtaa actcgcccgg ttcggcaagg cgcgcgccca attccaaaca gcggttcagc
     1141 agcatatcca tcaccaccgg cccgccgtgt ccctgaagct cgataacatc ttcgcccgta
     1201 aaacttgccg gtgcggcaaa aaacagcaaa agcccgctgt cgattgcctg accgtccgcg
     1261 tccgtaaaat cagcataggt tgcggtacgc ggtttgggcg ttttcccaca caaagcctct
     1321 gccatcggca gcaggttttt ccccgatatg cgtatcacgc ccacgccgcc gcgcccgggt
     1381 gcggtagcga ctgccgcaat cgttggaacg ttatccgaca taaaaccccc gaaaattcaa
     1441 aacagccgcg attatagcaa atgccgtctg aagtccgacg gtttggcttt cagacggtat
     1501 aaaaccgcaa aaatgcttga taaatccgtc cgcctgacct aatataacca tatggaaaaa
     1561 cgaaacacat acgccctcct gctcggtata ggctcgctgt tgggcctgtt ccatcccgca
     1621 aaaaccgcca tccgccccaa tcccgccgac gatctcaaaa acatcggcgg cgattttcaa
     1681 cgcgccatag agaaagcgcg caaatgaccg aaaacgcaca ggacaaggcg cggcaggctg
     1741 tcgaaaccgt cgtcaaatcc ccggagcttg tcgagcaaat cctgtccgac gagtacgtgc
     1801 aaataatgat agcccggcgt ttccattcgg gaccgttgcc gccgccgtcc gacttggcgc
     1861 aatacaacga cattatcagc aacggggcag accgcattat ggcaatggcg gaaaaagaac
     1921 aagccgtccg gcacgaaacc atacggcaag accaaacctt caacaggcgc gggcaactgt
     1981 acggcttcat cagcgtcatc ctgatactgc tttttgccgt cttcctcgta tggagcggct
     2041 accccgcaac cgccgcctcc cttgccggcg gcacagtggt tgccttggcg ggtgctttcg
     2101 tgattggaag aagccgagac caaggcaaaa attaatgaca aatcctaggg cgtgcttcat
     2161 atccgcccga acgccgaacc gcacatatag gcacatcccg cgcgccgccc ggaagcggaa
     2221 gccgcgccct cccaaacaaa cccgaatccc gtcagataag gaaaaataat gaaaacaacc
     2281 gacaaacgga caaccgaaac acaccgcaaa gccccgaaaa ccggccgcat ccgcttctcg
     2341 cctgcttact tagccatatg cctgtcgttc ggcattcttc cccaagcctg ggcgggacac
     2401 acttatttcg gcatcaacta ccaatactat cgcgactttg ccgaaaataa aggcaagttt
     2461 gcagtcgggg cgaaagatat tgaggtttac aacaaaaaag gggagttggt cggcaaatca
     2521 atgacaaaag ccccgatgat tgatttttct gtggtgtcgc gtaacggcgt ggcggcattg
     2581 gtgggcgatc aatatattgt gagcgtggca cataacggcg gctataacaa cgttgatttt
     2641 ggtgcggaag gaagaaatcc cgatcagcac cgtttttctt accaaattgt gaaaagaaat
     2701 aattataagc ctgacaattc acacccttac aacggcgatt accatatgcc gcgtttgcat
     2761 aaatttgtca cagatgcaga acctgtcgaa atgacgagtg acatgagggg gaatacctat
     2821 tccgataaag aaaaatatcc cgagcgtgtc cgcatcggct caggacacca ctattggcgt
     2881 tatgatgatg acaaacacgg cgatttatcc tactccggcg catggttaat tggcggcaat
     2941 acacatatgc agggttgggg aaataatggc gtagttagtt tgagcggcga tgtgcgccat
     3001 gccaacgact atggccctat gccgattgca ggtgcggcag gcgacagcgg ttcgccaatg
     3061 tttatttatg acaaaacaaa caataaatgg ctgctcaacg gagttttaca aaccggctac
     3121 ccttattccg gcagggaaaa cggtttccag ctgatacgca aagattggtt ctacgatgac
     3181 atttacagag gcgatacaca taccgtcttt tttgaaccgc gcagtaacgg acatttttcc
     3241 tttacatcca acaacaacgg tacgggtacg gtaacagaaa ccaacgaaaa ggtttccaat
     3301 ccaaagctta aagtacagac agtccgactg tttgacgaat ctttgaatga aactgataaa
     3361 gaaccagttt acgcggcagg gggtgttaat cagtaccgtc caaggttaaa caacggtgaa
     3421 aacctttctt ttatcgatta cggcaacggc aaactcatct tatcaaacaa catcaaccaa
     3481 ggcgcgggcg gtttgtattt tgaaggtgat tttacggtct cgcctgaaaa caacgaaacg
     3541 tggcaaggcg cgggcgttca tatcagtgaa gacagtaccg ttacttggaa agtaaacggc
     3601 gtggcaaacg accgcctgtc caaaatcggc aaaggcacgc tgcacgttca agccaaaggg
     3661 gaaaaccaag gctcgatcag cgtgggcgac ggtacagtca ttttggatca gcaggcagac
     3721 gataaaggca aaaaacaagc ctttagtgaa atcggcttgg tcagcggcag gggtacggtg
     3781 caactgaatg ccgataatca gttcaacccc gacaaactct atttcggctt tcgcggcgga
     3841 cgtttggatt taaacgggca ttcgctttcg ttccaccgta ttcaaaatac cgatgaaggg
     3901 gcgatgattg tcaatcataa tgccacaaca acatccaccg ttaccattac agggaatgaa
     3961 agtattacac aaccgagtgg taagaatatc aatagactta attacagcaa agaaattgcc
     4021 tacaacggtt ggtttggcga gaaagatacg accaaaacga acgggcggct caaccttgtt
     4081 taccagcccg ccgcagaaga ccgcaccctg ctgctttccg gcggaacaaa tttaaacggc
     4141 aacatcacgc aaacaaacgg caaactgttt ttcagcggca gaccgacacc gcacgcctac
     4201 aatcatttag gaagcgggtg gtcaaaaatg gaaggtatcc cacaaggaga aatcgtgtgg
     4261 gacaacgact ggatcaaccg cacgtttaaa gcggaaaatt tccatattca gggcgggcag
     4321 gcggtgattt cccgcaatgt tgccaaagtg gaaggcgatt ggcatttgag caatcacgcc
     4381 caagcagttt ttggtgtcgc accgcatcaa agccatacaa tctgtacacg ttcggactgg
     4441 acgggtctga caaattgtgt cgaaaaaacc attaccgacg ataaagtgat tgcttcattg
     4501 actaagaccg acatcagcgg caatgtcagc cttgccgatc acgctcattt aaatctcaca
     4561 gggcttgcca cactcaacgg caatcttagt gcaaatggcg atacacgtta tacagtcagc
     4621 cacaacgcca cccaaaacgg caaccttagc ctcgtgggca atgcccaagc aacatttaat
     4681 caagccacat taaacggcaa cacatcggct tcgggcaatg cttcatttaa tctaagcaac
     4741 aacgccgcac aaaacggcag tctgacgctt tccgacaacg ctaaggcaaa cgtaagccat
     4801 tccgcactca acggcaatgt ctccctagcc gataaggcag tattccattt tgaaaacagc
     4861 cgctttaccg gacaactcag cggcagcaag gatacagcat tacacttaaa agacagcgaa
     4921 tggacgctgc cgtcaggcac ggaattaggc aatttaaacc ttgacaacgc caccattaca
     4981 ctcaattccg cctatcgcca cgatgctgca ggcgcgcaaa ccggcagtgt gtcagacacg
     5041 ccgcgccgcc gttcgcgccg ttccctatta tccgttacac cgccaacttc ggtagaatcc
     5101 cgtttcaaca cgctgacggt aaacggcaaa ttgaacggtc aaggaacatt ccgctttatg
     5161 tcggaactct tcggctaccg aagcgacaaa ttgaagctgg cggaaagttc cgaaggcact
     5221 tacaccttgg cggtcaacaa taccggcaac gaacccgtaa gcctcgatca attgacggta
     5281 gtggaaggga aagacaacaa accgctgtcc gaaaacctta atttcaccct gcaaaacgaa
     5341 cacgtcgatg ccggcgcgtg gcgttaccaa ctcatccgca aagacggcga gttccgcctg
     5401 cataatccgg tcaaagaaca agagctttcc gacaaactcg gcaaggcaga agccaaaaaa
     5461 caggcggaaa aagacaacgc gcaaagcctt gacgcgctga ttgcggccgg gcgcgatgcc
     5521 gccgaaaaga cagaaagcgt tgccgaaccg gcccggcagg caggcgggga aaatgtcggc
     5581 attatgcagg cggaggaaga gaaaaaacgg gtgcaggcgg ataaagacag cgccttggcg
     5641 aaacagcgcg aagcggaaac ccggccggct accaccgcct tcccccgcgc ccgccgcgcc
     5701 cgccgggatt tgccgcaacc gcagccccaa ccgcaacctc aaccccaacc gcagcgcgac
     5761 ctgatcagcc gttatgccaa tagcggtttg agtgaatttt ccgccacgct caacagcgtt
     5821 ttcgccgtac aggacgaatt ggaccgcgtg tttgccgaag accgccgcaa cgccgtttgg
     5881 acaagcggca tccgggacac caaacactac cgttcgcaag atttccgcgc ctaccgccaa
     5941 caaaccgacc tgcgccaaat cggtatgcag aaaaacctcg gcagcgggcg cgtcggcatc
     6001 ctgttttcgc acaaccggac cgaaaacacc ttcgacgacg gcatcggcaa ctcggcacgg
     6061 cttgcccacg gcgccgtttt cgggcaatac ggcatcggca ggttcgacat cggcatcagc
     6121 acgggcgcgg gttttagcag cggcagtctt tcagacggca tcggaggcaa aatccgccgc
     6181 cgcgtgctgc attacggcat tcaggcacga taccgcgccg gtttcggcgg attcggcatc
     6241 gaaccgtaca tcggcgcaac gcgctatttc gtccaaaaag cggattaccg ctacgaaaac
     6301 gtcaatatcg ccacccccgg tcttgcgttc aaccgctacc gcgcgggcat taaggcagat
     6361 tattcattca aaccggcgca acacatttcc atcacgcctt atttgagcct gtcctatacc
     6421 gatgccgctt cgggcaaagt ccgaacacgc gtcaataccg ccgtattggc tcaggatttc
     6481 ggcaaaaccc gcagtgcgga atggggcgta aacgccgaaa tcaaaggttt cacgctgtcc
     6541 ctccacgctg ccgccgccaa aggcccgcaa ctggaagcgc aacacagcgc gggcatcaaa
     6601 ttaggctacc gctggtaacc gccggatatg ccgaaagggg tctgacgatg ccaccgtgcg
     6661 ctgtcaaacc ctttttctgc cgccgccttg tgcctgctcc attgtctgat aaccgtcaaa
     6721 ttgattttta gcccatgttt ggtgggtcgg ggaaatctat atcttcgtcc gtgccgaaat
     6781 agtctgagac ctttgcaaaa ttcctttccc tcccgacagc cgaaacccaa acacaggttt
     6841 tcgtctattt tcgccccaaa tacctcctaa ttctacccaa ataccccctt aatcctccac
     6901 ggacacccga taatcaggca tccgggctgc tttttaggcg gcagcgggcg cacttagcct
     6961 gttggccgct ttcaaaaggt tcaaacacat cgccttcaga tggctttgtg cactcacttt
     7021 gctcagcccg aaataggctg cccgcgcata gcggaattta cggtgcagcg taccgaagct
     7081 ctgttcgacc acatatagtg gattaaattt aaaccagtac ggtgttgcct cgccttgccg
     7141 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttaaattta atccactata
     7201 acgggtcttc gataaatatc ggttacgttt ggtttgcgtt tccgtcagcg gacggttgcg
     7261 gcaggctttg cgcataatgc cgttctgcaa ccgatgttct ttcagatgtt gccggttttc
     7321 cttgctgtcg tagcctttgt cggcatagac ggtcgtacct ttgggcagtc cttccaacaa
     7381 cggcgacagg tgtgtgcact catgggcatt ggtgggagtg atgtacagtt tctcgatata
     7441 gccttccgca tcggtacggg tatgttgttt gtaaccgagt ttgtagaggc tgtttttctt
     7501 tgtccaacgg gcatttttgt ccttactcag tgtggtttga ccgctgactt gtccctcttc
     7561 atcgacttct atggcctgac gctgttcgct gccg
//
LOCUS       DAWFAN010000093         7546 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWFAN010000093 DAWFAN010000000
VERSION     DAWFAN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.093"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            24..419
                     /locus_tag="WGO65_001384"
     CDS             24..419
                     /locus_tag="WGO65_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0327673.1"
                     /translation="MFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAGHGI
                     LYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLF
                     AAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WGO65_001385"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WGO65_001385"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ0327674.1"
                     /translation="MFQKHLQKASGSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKVIGCLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIAATLADMAELFPERRLMLAREITKTFETFLSGTPGELQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1375..1722
                     /locus_tag="WGO65_001386"
     CDS             1375..1722
                     /locus_tag="WGO65_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ0327675.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGIILFVEVKYRKNGRFGGSAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIKNITG"
     gene            1727..2320
                     /locus_tag="WGO65_001387"
     CDS             1727..2320
                     /locus_tag="WGO65_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ0327676.1"
                     /translation="MTTLPERVAAHFAESIRAKQEAGEVLAEPAAQAAEMMLQCLMND
                     GKILICGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDYIF
                     SKQVRALGRAGDILVGISTSGNSANVAEAVKAAHEHDMRVVALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            2384..2992
                     /locus_tag="WGO65_001388"
     CDS             2384..2992
                     /locus_tag="WGO65_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ0327677.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3000..3644
                     /locus_tag="WGO65_001389"
     CDS             3000..3644
                     /locus_tag="WGO65_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327678.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3684..4463
                     /gene="map"
                     /locus_tag="WGO65_001390"
     CDS             3684..4463
                     /gene="map"
                     /locus_tag="WGO65_001390"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ0327679.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4618..4917
                     /locus_tag="WGO65_001391"
     CDS             4618..4917
                     /locus_tag="WGO65_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0327680.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5155..5529
                     /locus_tag="WGO65_001392"
     CDS             5155..5529
                     /locus_tag="WGO65_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0327681.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5717..7183)
                     /locus_tag="WGO65_001393"
     CDS             complement(5717..7183)
                     /locus_tag="WGO65_001393"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ0327682.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1923 a         2296 c         1858 g         1469 t
ORIGIN      
        1 aagaggcgga atgggcgggc agcctgttcg gcctgcacaa atctttcggc ttcctcacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cagttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 attgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcgccgcgcg attggttgcc
      661 gtccgccgcc aatgccgtct gaagctcccc cggcgtgccg cttaagaacg tttcaaacgt
      721 tttggtgatt tcgcgcgcca gcattaatcg gcgttcgggg aacagttccg ccatatcggc
      781 aagcgtcgcc gcaattcggt gcggcgtttc aaacatcacg acgggaaacg ccgcccgcac
      841 ccatttggca aacagtttcc tgcgttctcc cgatttcggc ggcacaaaac cgttgaaata
      901 aaaatcggat ccctccacac cggccacgct caaagccgcc attaccgcgc ttgcgcccac
      961 gacggggaca actttaaacc ccgcctcgcg cacgcggcgg gcgagtttcg cgcccgggtc
     1021 gcacacagcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagaca
     1081 gccgataacc ttgtccgcca tctgccgttc gttgtgttcg cgcacgctga cgagtttgcc
     1141 ctgaatgccg tacgcactca aaagctgcgc agtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcacg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgccg gaggctttct gcaagtgttt
     1321 ctgaaacata agaatgccgt ctgaaaaaca aacattataa aggttaaacc gattatgcgc
     1381 ttaaaccaca aacagggcga ggcaggcgaa gatgccgcgc ttgccttcct ccaatcccaa
     1441 ggctgcacgc tgcttgcccg caactggcac tgcgcctacg gcgaaatcga cctgattgtc
     1501 aaaaacggcg gcataatact gttcgttgaa gtaaaatatc gcaaaaacgg gcggttcggc
     1561 ggttcggcat acagcatttc gccgtccaaa ttattgaaac tgcaacgaag tgtagagtat
     1621 tatctgcaac agaacaggct gaccaacgta ccgtgccgcc tcgacgcggt actcatcgaa
     1681 ggcagccgac cgcccgagtg gataaagaat attacaggtt gacgatatga cgacattacc
     1741 agaacgcgtt gccgcccatt ttgccgaaag catccgcgcc aaacaggaag ccggggaagt
     1801 gctcgccgaa cccgccgcgc aggctgccga gatgatgctg caatgcctga tgaacgacgg
     1861 caaaatcctg atatgcggca acggcggttc ggctgccgac gcacagcatt tcgccgccga
     1921 aatgaccggg cgttttgaaa aagaacgtat ggaactcgcc gccgtcgccc tgaccaccga
     1981 cacgtccgcc ctgaccgcca tcggcaacga ctacggcttc gattatatct tcagcaaaca
     2041 agtgcgcgcc ctcggacgcg ccggcgacat cctcgtcggc atttccacct ccggcaattc
     2101 cgccaacgtc gccgaagccg tcaaagccgc ccacgaacac gatatgcgcg tcgtcgcact
     2161 gaccggccgc gacggcggca aaatcgccgc gatgctcaaa gacaccgacg tactgctcaa
     2221 cgtcccccat ccgcgcaccg cccgcattca ggaaaaccac atcctgctga tacacgccat
     2281 atgcgactgt atcgactccg tactgctgga aggaatgtaa cccttttcag acggcatggc
     2341 gcaaagcaat gccgtctgaa acgcccaaga aaggaagccc ccgatgaaac ccaaaccgca
     2401 caccgtccgc accctgattg ccgccatttt cagccttgcc cttagcggct gcgtcagcgc
     2461 agtaatcgga agcgccgccg tcggcgcgaa atccgccgtc gaccgccgaa ccaccggcgc
     2521 gcaaaccgac gacaacgtta tggcgttgcg tatcgaaacc accgcccgct cctatctgcg
     2581 ccaaaacaac caaaccaaag gctacacgcc ccaaatctcc gttgtcggct acaaccgcca
     2641 cctgctgctg ctcggacaag tcgccaccga aggcgagaaa cagttcgtcg gtcagattgc
     2701 acgttccgaa caggccgccg aaggcgtata caactacatt accgtcgcct ccctgccgcg
     2761 cactgccggc gacatcgccg gcgacacttg gaacacatcc aaagtccgcg ccacgctgtt
     2821 gggcatcagc cccgccacac aggcgcgcgt caaaatcgtt acctacggca acgtaaccta
     2881 cgttatgggc atcctcaccc ccgaagaaca ggcgcagatt acccaaaaag tcagcaccac
     2941 cgtcggcgta caaaaagtca tcaccctcta ccaaaactac gtccaacgct gactcggcaa
     3001 tgccgtctga accgccttca gacggcattg cccgacaccc caaaagcaca atcaaaatgg
     3061 caaaaaaacc gaacaaaccc ttcaggctga cccccaaact cctgatacgc gtcgtattgc
     3121 tcatctgtat caccgccatc ggcgcattgg caataggcat cgtcagcaca ttcaacccga
     3181 acggcggcaa aaccctccaa accgaaccgc aacacaccga cagcccccgc gaaaccgaat
     3241 tctggctgcc aaacggcgta gtcggacaag atgccgccca acccgaacac caccacgccg
     3301 cctcatccga acccgcacag ccggacggca cagacgaaag cggcagcgga ctgccgtccc
     3361 ctgccgcacc caagaaaaac cgggtcaaac cgcaacctgc cgacacagct caaaccgaca
     3421 ggcagccgga cgacgccgga acacaagctg aaaacacact caaagaaacc cccgtactgc
     3481 ccacaaacgt cccccgtccc gaaccccgaa aagaaacacc cgaaaaacag gcgcagccca
     3541 aagaaacgcc caaagaaacg cccaaagaaa accataccaa accggacacc ccgaaaaaca
     3601 cgccgcccaa accccataaa gaaattctcg acaacctctt ctgacccggc acggcaggca
     3661 cacccgcaat ccaaggaagc attatgaacg gcatcatcat caaaaccccc gaagaaatcg
     3721 aaaaaatgcg cgagctgggc aaactcgtcg ccgaagccct cgactacatc ggacaattcg
     3781 tcaaacccgg cgtaaccacc gacgaaatcg acaaactcgt ttacgactac cacgtcaacg
     3841 tccaaggcgg ctatcccgcc cccctgcact acggcaaccc gccctacccc aaatcctgct
     3901 gcacctccgt caaccacgtc atctgccacg gcattcccga cgacaagccg ctcaaagaag
     3961 gcgacattat caacatcgac ctcaccatca aaaaagacgg cttccacggc gactccagcc
     4021 gtatgtttac cgtcggcaaa gtctccccca tcgcccaacg cctgatcgac gtaacccacg
     4081 cctccatgat ggcgggcata gaagccgtca aacccggcgc gacactgggc gacgtaggtt
     4141 acgcctgcca acaggttgcc gaaaacgccg gctattccgt cgtacaggaa ttctgcggac
     4201 acggcatcgg gcgcggtttc cacgaagccc cgcaagtgtt gcactacgga aaaaaaggac
     4261 agggctccgt tctaaaaccg ggtatgattt ttaccgtcga accgatgatc aaccaaggca
     4321 aacgccacct gcgtatcctc aacgacggct ggacggtggt taccaaagac cgctccctct
     4381 ccgcccaatg ggaacacgaa gtcttggtga ccgaaaccgg ctacgaaatc ctcaccgtca
     4441 gccccgcctc cggcaaaccc tgaagccgga cgtatccgcc ccataaaaac aaacaatgcc
     4501 gtctgaaaga aacggcagat atgatatata atataaaaac aggcttgacc cggcacatta
     4561 cgaaaacaaa gcaaatcgga atttgccccg caaccagaca aacttaaagg aagttttatg
     4621 aaaatatttg aaaatataga agatgttaaa gccatccgta aaaagaccgg gctgaaccag
     4681 atagacttct ggggcaaggt cggcgttacc cagtctggag gctcgcgcta cgaaaccggc
     4741 cgcaaaatgc ccaaacccgt acgcgaactg ctccgcctcg tccatatcga atgcatcgat
     4801 ttggcgaaag tcaacaaaaa agatatggaa atcgccgccc tgttgaaaaa acaccatccc
     4861 gacctgtatg ccgagttgtc caaacagacc aagtccgaaa gaaaaaaaca aagttaaacc
     4921 gcaacctccg gatgcccgac agtttttcat ttccgaaaaa cgcaaacaat gccgtctgaa
     4981 acaccggaca ggccgccgta tcccgcctgc cgcccctgcc tcaaaccgcc gaaccgcccg
     5041 aacccgcctt tttacaaact ttatccaatt tcctgtttat ttcaggatac gccgacatta
     5101 gaatgtcaaa cagctcgaaa cgggcaaact ccacatccat ccaaaggaat aaaaatgaaa
     5161 cttctgacca ccgcaatcct gtcttccgca atcgcgctca gcagtatggc tgctgctgcc
     5221 ggcacgaaca accccaccgt tgccaaaaaa accgtcagct acgtctgcca gcaaggtaaa
     5281 aaagtcaaag taacctacgg cttcaacaaa cagggtctga ccacatacgc ttccgccgtc
     5341 atcaacggca aacgtgtgca aatgcctgtc aatttggata aatccgacaa tgtggaaaca
     5401 ttctacggca aagaaggcgg ttatgttttg ggtaccggcg tgatggatgg caaatcttac
     5461 cgcaaacagc ccattatgat taccgcacct gacaaccaaa tcgtcttcaa agactgttcc
     5521 ccacgttaat caggcaacaa aaaacagcgt tttcagaaat gaaaacgctg tttttttgac
     5581 cgttccatta ttcgcaaaag ggaaaaaacg attacctgcc ccgtgtatca aaacctgccc
     5641 tgccggatga agggcataac cggcagggac ggcgtcaaca ccatatgggg gtacggcttt
     5701 tcttgaaaga ttcggcttaa atatccaata ctttcgcggt ataggcgata atttcatccg
     5761 ccctttcagg gttttcgttc aacttgatgc cgtaacccgg taccagctct ttcagacggt
     5821 cttcccaaga cggggcgcgc tcggggaagc attggtgcat cagccggatc atcagcggca
     5881 cagcggtcga tgcgcccggc gacgcgccca gcaatgcggc gagtgagccg tcggcgtggg
     5941 cgacaatctc cgtaccaaac tggagcacgc cgcctttttc ggagtctttt ttaatgattt
     6001 ggacgcgttg ccctgcggtg atgagttccc agtcgtcggg gtttgcctcg gggtagtatt
     6061 ccagcaggga ggcgaagcgt tcttctttgg ttttacgcaa ttcgcccagc aggtatttgg
     6121 tcagcggcat attcgcccag ccggcgcaca gcataggata gaggttgtcc atatggatgg
     6181 acagcggcaa atccataagc gagccttgct tgaggaagtt ggaacggaag cctgcgtaag
     6241 ggccgaacat aaggtggcgt ttgccgtcca cgttgcgtgt gtcgaggtgc gggacggaca
     6301 tcggcggcgc gccgacggaa gcctgcccgt acactttggc gttgtgttgt tcggcggttt
     6361 cggggttgct gttgcggaag aacaggccgg acacggggaa gccgccgtag cctttgcctt
     6421 cggggatgcc ggatttttgc agcagggtca gcgcgccgcc gcccgcgccg aggaagagga
     6481 agcgggtacg gagggtgagc tgcccgtcgg ggttgcgggt atcggcggtt ttgagcaccc
     6541 acgcgccgtc ggattcgcgt ttgatgtctt cgacgtggcg gttgaactcg gtttttacgc
     6601 ccttgccctg caaatatttc accatttggc gcgtcagccg tccgaaatcg acatccgtac
     6661 cttcggcgga gtagttggcg gcgacgggtt ggttttcgtc ccggccgcgc atcatcagcg
     6721 gagcccaatc ggaaattttg ttccgatcgg tggaaaattc catattttca aaaagttttt
     6781 gggttttaaa cgcgtcataa cgtttttgaa gataagaaca atggtcttca ttcatcacca
     6841 aagacatatg cggcacggca ttgatgaagg aattgtcttc caacttgcct tccgcgacca
     6901 gcgtcgccca aaactggcgg ctgacatgaa actgttcggc aatattgagg gcgcgcgccg
     6961 gatcgataat cccatttgca cccaacggcg catagttcaa ttcgcacagc gcggaatgcc
     7021 ccgtgccggc gttgttccac gcgtttgacg attccaacgc cacatcttcc aagcgttcaa
     7081 tcagggtgat ttcccaagac ggttcgagtt ctttgagcaa aacgcccaaa gtcgcgctca
     7141 taatgccgcc gcccaccaag acaacgtctg tcgcttcagc catggtttac tcctaaaaaa
     7201 caggcatctt ctgcccttat ggttatttgc cgtactacaa acgcctgaat cgcaaaagca
     7261 gggaaaaccg gcaatggtgt gtgtccgagt atgctgtttc ggggttggaa tgcgttgcaa
     7321 gtatggcttc cgacaccgct tcaggggctt gtaatatgtt atcgtgaatg tagtggattt
     7381 tactgggaaa tgcaaagttt ttctgtcgcc cgccaagtcg ggaaactgcg aaatgaaaaa
     7441 taaaaatagt tatttatcta tatatatcaa atttttaata gataaaaaat caaaattgtt
     7501 tatatattaa tttttaaaag attgtcagca tattgcgtta agtttt
//
LOCUS       DAWFAN010000094         7453 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWFAN010000094 DAWFAN010000000
VERSION     DAWFAN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.094"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            155..328
                     /locus_tag="WGO65_001394"
     CDS             155..328
                     /locus_tag="WGO65_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0327683.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
     gene            392..1675
                     /gene="hemL"
                     /locus_tag="WGO65_001395"
     CDS             392..1675
                     /gene="hemL"
                     /locus_tag="WGO65_001395"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ0327684.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGSAIVGHAHPEVIEAVREAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAE"
     gene            complement(1891..2163)
                     /locus_tag="WGO65_001396"
     CDS             complement(1891..2163)
                     /locus_tag="WGO65_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ0327685.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2174..2737)
                     /gene="orn"
                     /locus_tag="WGO65_001397"
     CDS             complement(2174..2737)
                     /gene="orn"
                     /locus_tag="WGO65_001397"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ0327686.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPERTTP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2755..3642)
                     /gene="prmA"
                     /locus_tag="WGO65_001398"
     CDS             complement(2755..3642)
                     /gene="prmA"
                     /locus_tag="WGO65_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ0327687.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGETIEDQDWVR
                     LTQAQFDPIRISERLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGVKR"
     gene            complement(3760..5121)
                     /gene="accC"
                     /locus_tag="WGO65_001399"
     CDS             complement(3760..5121)
                     /gene="accC"
                     /locus_tag="WGO65_001399"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ0327688.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEEERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(5234..5689)
                     /locus_tag="WGO65_001400"
     CDS             complement(5234..5689)
                     /locus_tag="WGO65_001400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ0327689.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5671..5928)
                     /locus_tag="WGO65_001401"
     CDS             complement(5671..5928)
                     /locus_tag="WGO65_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ0327690.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6045..7085
                     /gene="queA"
                     /locus_tag="WGO65_001402"
     CDS             6045..7085
                     /gene="queA"
                     /locus_tag="WGO65_001402"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ0327691.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         1551 a         1875 c         2217 g         1810 t
ORIGIN      
        1 gacggaagag ggaatcgcac aaacggttgt taaaggcaaa acgactcggg cggaggtaga
       61 ggcacgtttc gggaagcgca accctttcgg ttgttatgcc tatcatgaag tcagcctgcc
      121 gatttataat tttttgccga ccaatttcat ttatatgaaa tcagaacgcc ggcattggga
      181 gtggtgtgtg gattacgact gggaaggagt tgtccgggac taccgcttta cacataaagt
      241 ggaaaaagac gagcgttccg tcatccggga tgcggttggc gtcatccgca aagaagcagg
      301 caaatccttg tcgcaacctg aaaaatgata aaatgggact ttctgcttct aagcccgaaa
      361 cctgccgttc agacggcatt tgaggataaa tatgaaccgt aacgaaattt tattcgaccg
      421 cgccaaagcc atcatccccg gcggcgtgaa ttcccccgtc cgcgcattcg gcagcgtcgg
      481 cggcgtaccg cgcttcatca aaaaagccga aggcgcgtat gtttgggatg aaaacggcac
      541 gcgctacacc gactatgtcg gctcgtgggg gtctgcgatt gtcggccacg cgcaccccga
      601 agtcatcgaa gccgtgcgcg aagccgcgtt gggcggtttg tcgttcggcg cgcctaccga
      661 gggcgaaatc gtcatcgccg aagaaatcgc caaaatcatg ccgtccgtcg aacggctgcg
      721 cctcgtcagc tccggtaccg aagcgaccat gaccgccatc cgtctggcac gcggctttac
      781 cggccgtgac aaaatcatca aatttgaagg ctgctaccac ggccattccg acagcctgtt
      841 ggtgaaagca ggcagcggtc tgctcacctt cggtaatccc tcttccgccg gtgtgcctgc
      901 cgactttacc aaacacactt tagtcctcga atacaacaac atcgcccaac tcgaagaagc
      961 ctttgctcaa agcggcaatg acattgcctg tgtgattttg gagccttttg tcggcaatat
     1021 gaatctggtt aggccgtctg aagccttcgt caaagccctg cgcgaattga ccgaaaaaca
     1081 cggcgcagtg ttgatttacg acgaagtgat gaccggtttc cgcgtcgcgc tcggcggcgc
     1141 acaatcgctg cacggcatca cgcccgacct gaccacgatg ggcaaagtca tcggcggcgg
     1201 tatgccactt gccgcgttcg gcggacgcaa agacattatg gaatgtattt ccccgctggg
     1261 cggcgtgtat caggcaggca ccttgtcggg caacccgatt gccgtcgccg ccggtttgaa
     1321 aacgctggaa atcatccgcc gcgaagggtt ttatgaaaac ctgacggcgc gtaccgaaca
     1381 gttggttcaa ggttttcgga cggcagcgga tgcggcgggc atcgagttca ccgccgacag
     1441 cgtgggcggt atgttcggtc tgtatttcgc cgcacacgcg ccgcgaaact atgccgatat
     1501 ggcgcgttcc aatattgaag gcttcaaaca gtttttccac gggatgctcg accgcggcat
     1561 tgcctttggc ccgtccgctt atgaagcagg tttcgtttcc gccgcgcata cgcccgagct
     1621 gattgacgaa acggttgcgg ttgcggttga agtgttcaag gcgatggctg aatgatgttt
     1681 tgacggacag ggtttctctg ttcgatctgt ttggcagatt gaagtaagaa tgcacaccgc
     1741 cgtcattccc gcgcaggcgg gaatctagac ctttcagttt ctgtaatgat tgaaaataac
     1801 gggccgaaat cagccgcgcc cggcaggaag ccgccgccac ctgaaacaat gccgtctgaa
     1861 gccttcagac ggcattttat cggaagcgca tcagaaaaaa cggatagcga catatgcggt
     1921 aaaagcaagg gcaattacat agataaaccc ctttataaat tcaaaagtct catctatatc
     1981 gctatatttt tctttcagtt cgtcagattt tcgataacgt ttcattttca agaccacttc
     2041 atgaccaaca agcccgacct tccggattcc cgcctgcgcg ggaatgacgg gcgtgtatat
     2101 ttttgatttc aatctgctgt aaaaatgccg tctgaaatgc aggatttgcg cttcagacgg
     2161 cattttttgt ttcttattgc gcttcggctc tcggggcgga aatcagaaag tgttcgcggt
     2221 agtggcgcat ttcttcgatg ctctccaaaa tgtcgtccaa tgccttgtgc gaaccgcgtt
     2281 tgaccacgct tttggcaacg ggcggattcc agcgtttggc aagctctttg agcgtggaaa
     2341 cgtcgaggtt gcggtagtgg aagtagtttt ccagtttcgg catatatttg accataaaac
     2401 gccggtcttg gtggatggaa ttgccgcaca tcggcgtggt gcgttcgggt acccattccg
     2461 acataaagtc cagcagtttc tgttcgactt cggcttcggt atgcgacgat tcgcgtacgc
     2521 gctgcgtcag ccccgtcctg ccgtgtgtgg cggtgttcca ctcgtccata ttgtcgagca
     2581 gctcgtcact ttggtggacg gcgtaaactt cggattgcgc caatacattc aaatccgagt
     2641 cggtaataat catcgcgact tcgataatgc ggtcggtttc gggattaagc cccgtcattt
     2701 ccatatcgag ccagcagagg ttgtttttat cctgcacggt gtttcctttc cgtttcagcg
     2761 ttttacgccg ctcaatcgcg cccatccttc ttcggtttcc gcagggtcga ggtcgaacca
     2821 ttggctgtaa atgccgccga gttcttcggc ttgttcgtcc aacaaaccgg ataacacgat
     2881 gcggccgcct tgtttggtgc gggcggcgag catttcgccg agcatacgta aagggttggc
     2941 gaggatgttg gcgacaacta cgtcgaattg cccttgaggc agaccgtcgg gcaggaagaa
     3001 ttgtgcatcg acgttgttct gttcggcgtt gtccttgccg gcgcggacgg cttgttcgtc
     3061 aatatccacg ccgacggcag aacctgcgcc gagtttgagg gcggcgatgg tcaggatgcc
     3121 cgaaccgcag ccgtagtcga ggacgctttc gccgtttttg agttgcgtat ccagccattt
     3181 gaggcagagg cgcgtggtcg ggtggctgcc ggtgccgaag gcgaggccgg gatcgaggcg
     3241 taggttgacg gcagtgcctt cgggggcttc gtgccaagag ggggtaatcc acaggcgttc
     3301 ggaaattcgg atggggtcga attgcgcttg ggtcaggcgt acccagtctt ggtcttcgat
     3361 ggtttcgccg gtgtatgcca agtcttttaa cccgcattct tgtgcggcgg tttggatgat
     3421 ggcggcggct tcgtcgtgtt cgccgaacag ggcgatgact ttgctctgct gccagatttg
     3481 ttcggcgggc ataccgggtt cgccgaaaat cgcctgttcg ttttgcgtac cggcgtaggc
     3541 atcttcgatg gcggcggaga gtgcgccgtg ttccatcagc gcgtcggcga ggcgttcggc
     3601 gacggcatcg ttgacgttga cggtgatttg ttggtagggc atagcgggct ttcttaacag
     3661 accttcggaa tagggtcgta ctgtataagg ggatgggtgt gtaacggaaa tgccgtctga
     3721 aaacggacaa actgtttcag acggcatatc ggggacggct tatttgtcct gtttggcttt
     3781 gcgatcttcc agccagtgtt ccaaatagtg gatgctgacg ccgccttttt ggaaacccgc
     3841 atcggcgaac aggtcgcggt gaagcggcgt attggttttg atgccggtta ccgccagctc
     3901 ggcgagtgcg acgcgcattt tcgccattgc ctgttcacgc gttttgccgt gtacgcagat
     3961 tttgccgatc aggctgtcgt agtacggtgg gatgcggtag ccttggtaaa tgtggctgtc
     4021 cacgcggatg ccgaagccgc cgggcaggtg gcagctttca atcggccccg ggctggggat
     4081 gaagttgtac gggtcttcgg cgttgatacg gcactcaaac gcgtggcctt cgacttgaat
     4141 atccttttgt ttgtattgca acggcaggcc ggcggcgatg cgcaattgct cttgtacgat
     4201 gtccacgccg gtgatgagtt cggtaaccgg atgctcgacc tgaacgcgcg tgttcatctc
     4261 gataaagaaa aattcgccgt cttcgtacag gaattcaaac gtacctgcgc cacgatagcc
     4321 gatgcgtttg caggcgtcgg cacaggcgtt gccgattttg gcgcgttctt cttcagtgat
     4381 gaacggagcc ggtgcttcct cgatgacttt ttggtggcgg cgttgcagcg aacagtcgcg
     4441 ctcggcaaga tatacggcgt tgccgtgttc gtcggcaatc acttggattt cgacgtggcg
     4501 cggacgttgc agatagcgtt ccatgtaaac catcgggttg ccgaatgccg cgcctgcttc
     4561 tgccttggtc atttcgacag attggaggag gtcttctttt ttctcgacca cgcgcatacc
     4621 gcgaccgccg ccgccgccag aggctttgat aatgacggga taaccgactt tatcggcgat
     4681 tttgaggatt tcttcgccgt cgtcgggcaa tgcgccgtca gaaccgggga cgcagggtac
     4741 gcctgccgct atcatcgcgt gtttggcgga gactttgtcg cccatcaggc ggatggtgtc
     4801 gggtttcggg ccgataaagg taaagccgga ctgctcgacc tgttcggcga aatcggcgtt
     4861 ttcggcaagg aaaccgtagc ccggatggac agcgtccgcg cagcttactt cggcggcggc
     4921 gataatggcg gggacgttaa ggtagctttg cgcggaagcg gcagggccga tgcacacgga
     4981 ttcgtcggcg agtttgacgt gcaggctgtc tttgtcggcc tcggaatgca cggcgacggt
     5041 ggcaatgccc atttcgcggc aggcacggag tacgcgtaat gcgatttcgc ctcggttggc
     5101 gattaaaact tttttcagca tgatgactgt tcctgtggtt ccggcaaccc gtccgtaaaa
     5161 aaaaggtgcg ggaacgctga agggggaaag catttcagac ggcatcggaa gtttatgccg
     5221 tctgaaaaca ggattatccg atgatgaaga gcggttcgcc gaattcgacg ggcgtaccgt
     5281 tttcgaccaa aatttctttg accgtgccgg atttttcggc ttcgatttcg ttcatcagct
     5341 tcatcgcttc gatgatgcac agcgtgtcgc cggctttaac ctgttggccg acttcgacaa
     5401 aaggcgcggc attcgggccg ggtgcgcggt agaacgtgcc gaccataggt gatttttgcg
     5461 cgtcggacaa atcgcgggcg gcaggtgcgg cggcaggcgc ggatgccgca acgggtgcgg
     5521 cggcaggcgt tacggccggc gcggcggcag gtacgggcgc ggcgtaaacg ggtgcggcgg
     5581 cgatggttcg ggtgatgcgg actttttcct cgccttcggt tacttcgatt tcggcgatac
     5641 ccgattcttc aaccaaatca atcagttttt ttaatttgcg caaatccatt tctgttcctt
     5701 taaaggctgc cggtgttccg gcgggttgtt gcgttggttt ttcagaaaac agccggcttg
     5761 cggcaacggc gcaaaccggg cggaatggtg ttcggatacg gttttcctgt ggtggaaaac
     5821 aaccgtgaga atgtgtttat tttcccgaag ttgtcggtaa atgtccagta aaatatcaaa
     5881 aaacggcggt ttttggggga aatgtgcagg aagtttgatt ttgcgcacaa gatgttccag
     5941 gttaagagtg tttattctaa tctgttggtt tttcggacaa agatgccgtc tgaaaaaggg
     6001 ctaaagtgcg tataatggcg gcttgcccga acgagagtgt aaaaatggat atttcagatt
     6061 ttgattttac cctgcccgaa aagctgattg cccagcatcc gcccgaagtg cgcggcagca
     6121 gccgcctttt ggttgcgttg cccgatatgc cgctgcaaga ccgggtgttc ggcgatttgc
     6181 cggattatgt cgaggcgggc gacgttttgg tgttcaacaa caccaaagtc atgaaggcgc
     6241 ggctgtttgg gcagaaagac agcggcggca ggatcgaagc cctgattgag cgcgtgttgg
     6301 acaaccatac cgcactggcg cacatccgtt cgtccaagtc ccccaagccc ggtatggggc
     6361 tggtgtttga aggcggcatc cgtgccgtga tggtcgggcg tgagggcgaa ctgttctgcc
     6421 tgcgttttga aggcggtcaa accgtttacg aacttttgga acagaacgga cacctgcccc
     6481 tgccgcctta tatcgaacgc gccgccgacg cggacgacga cagccgttat caaaccgttt
     6541 atgccaaata tcagggtgcg gtcgccgcgc cgacggcggg tctgcatttt acggaagaac
     6601 ttttgcgccg cctgaaagac aaaggcgcag taaccgcaga agtaaccctg cacgtcggtg
     6661 cgggaacgtt ccagcccgtg cgtgtggaaa aaatcgaaga acacaaaatg cacagcgaat
     6721 ggtttgaagt gccgtctgaa accgccgccg ccgttgaggc ggcaaaagca cgggggaaca
     6781 aagtctgggc ggtcggcacg acttccatgc gcgccctcga atctgccgcg cgtgcgacgg
     6841 gtcgtctgaa agcggggcag ggcgacaccg atattttcat cacgccgggc taccgtttta
     6901 atgttgtcga caggctggtt accaattttc atttgccgaa atcgacgctg ctgatgcttg
     6961 tcagcgcgtt ttcgggtatg ggtcatatcc gcgccgcgta tcgttatgcg gtcgaacgcg
     7021 aataccgttt cttcagctac ggcgatgcga tggttttggg gcggaacgaa ggggtggtac
     7081 gttagaacag ccccaagtct attgggggaa gacaagtgcc gtctgaacgg acgttcggac
     7141 ggcattcttt tgttgatttg aatgaaagga actgtatgac atagattcag gcgcgagagg
     7201 cgagaaactc agtcattgtt gatgacgcgc aatacgttaa aattcattcc gttttggtcg
     7261 tataaacata ttacatacgc catttttttg tattcaaggc tgcctgaaaa cagtgggcaa
     7321 gaatatctaa cccaacgaaa agctgaaaaa gcaacaaaaa ataatctgca ccttagtcgg
     7381 ttggttagtc caaccttagg ggtgcagacc attcaatctt tcaggctgcc tttgtttgtt
     7441 tataagataa att
//
LOCUS       DAWFAN010000095         7272 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWFAN010000095 DAWFAN010000000
VERSION     DAWFAN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.095"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(108..2711)
                     /gene="pepN"
                     /locus_tag="WGO65_001403"
     CDS             complement(108..2711)
                     /gene="pepN"
                     /locus_tag="WGO65_001403"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ0327692.1"
                     /translation="MSKIVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPPEAELWDGT
                     ENIDPLCYHQAHEALLDTIAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2847..3641)
                     /locus_tag="WGO65_001404"
     CDS             complement(2847..3641)
                     /locus_tag="WGO65_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0327693.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(4391..5260)
                     /locus_tag="WGO65_001405"
     CDS             complement(4391..5260)
                     /locus_tag="WGO65_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ0327694.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWDGKKRETVLKQHFKHMAKLMLEYGLYWYAPAKRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKGSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPGEDAKADAQRMNRFIEDRVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5311..5847)
                     /gene="ruvC"
                     /locus_tag="WGO65_001406"
     CDS             complement(5311..5847)
                     /gene="ruvC"
                     /locus_tag="WGO65_001406"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0327695.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5850..6089)
                     /locus_tag="WGO65_001407"
     CDS             complement(5850..6089)
                     /locus_tag="WGO65_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ0327696.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6119..7129)
                     /gene="dusB"
                     /locus_tag="WGO65_001408"
     CDS             complement(6119..7129)
                     /gene="dusB"
                     /locus_tag="WGO65_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ0327697.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLIS
                     DPTLRNTKKTLHRSDFADEGGVVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHKNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDSAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     AF"
BASE COUNT         1614 a         1907 c         1927 g         1824 t
ORIGIN      
        1 atatgacttt gcggccgttt tgccattgcg taataaaacg atggggaagt gatgataaaa
       61 cgtgtgtgta actatatcag acggcattgt ttttctgttt gacggcctca atccaaaatt
      121 ttgccgacga tttcgcccac gtctttcgac aatccttcct gcgcccgaat gcgctgcaat
      181 gcttgtttca ccaagttttt gcggtgcggc tcgagcttat tgcagagatt gaacgcctgc
      241 actaggcggg cggcgacctg cgggttgaag cggtcgattt caatgacttt gtcggcgata
      301 aagcggtagc cgctgccgtc ttctgcgtgg aaatgcggga cgttgcggct gaagctgccg
      361 atgagcgaac gggctttgtt ggggttttcg aggctgaatt tcggatgctg caaggcggtt
      421 tgaacctgtt gcagggtgtc gctgcgacgg cttgagccga cgagggcgaa atatttgtcc
      481 atcaccagcg cgtcgtctga aaacttgtcg gcaaactgcg ccagcaggcg gttgcgcgta
      541 tcgctttcgt tgccgttgac ggcggacagg atgccccatt cgtgggtcat gttttgcgcc
      601 atttcgccgt atttttcggc aacggtttcg atgtgcgcgg ggtcggcgcg caggacgaag
      661 gcgcggcaga cgttgcgcag cgtgcgccag ccggcagctt cggggctgta ctcgtagctt
      721 tggttttcct gcttcgccgc ctgacggttc aattcgtgcc atttcggcag gaagcggacg
      781 gcgattgtat ccaacaaggc ttcgtgcgcc tgatggtagc acaacgggtc gatgttttcc
      841 gtgccgtccc acagctcggc ttcaggcggc acgcccaaaa gcagggcttt gaaggcgttg
      901 tctaagaggt cgtctgaaat gactttttcg acggcggcaa gcagtttttc gtgtttcggc
      961 aacccgatgc cgtctgaaag cgcggcaagg ttggcggcga cggcgcggcg gtagagcgtt
     1021 tgggcggctt cccagcgcgt gaaggcgtcg ctgtcgtggg cgagcaggag cagcaggtcg
     1081 tcgtcgctgt acggatagtt cagatgcact ggcgcgctga acccgcgcag cagcgaggga
     1141 acgacggctt cggttacgct ttcgaactgg aaggtctgtt cggcttcggt cagcagtaac
     1201 acggcttcgg ttgcgcgttt gccctgataa tcgaatgcca ccgcttcgcc gttgcggttc
     1261 agcagcccga ctttgacggg aatcatcatc ggctgcttgt ccgccatatc gggcgtgggc
     1321 ggcacggttt gtttgatggt taactcgaaa ctattgtttt tcagacgacc ttccgcttcc
     1381 aagacgggcg tacccgcctg gctgtaccac aaggcgaact ggtcgagatt gatgccgttc
     1441 gcgtccgcca tcgccgcgcg gaaatcgtcg caggtcacgg cctgcccgtc gtgtcgttgg
     1501 aaatagagct tcatgccttt ttggaagccc tcttcgccga gcaaggtgtg atacatccgc
     1561 accacttccg cgcctttttc ataaacggtc atggtgtaga aattgttcat ctcctcatag
     1621 ctggcggggc gcaccggatg ggcggtcggg cctgcgtctt cggggaactg gtgctggcgc
     1681 agcaggcgga tgttctcgat gcggcgcacg gcgcggctgg cgcggtcgcc ggaaaattct
     1741 tggtcgcgga acacggtcaa cccttctttc agcgaaagct ggaaccaatc gcggcaggta
     1801 acgcggttgc ctgtccagtt gtggaaatac tcgtgtccga ccacggattc gatgccttca
     1861 aaatcggtat cggtggcggt acggctgtcg gcaaggacga acttggtatt aaaaatgttc
     1921 agccccttgt tttccatcgc gcccatattg aaatcgccca cggcgacgac catgaaaata
     1981 tccaagtcgt attccaaacc gaagcgcgtt tcgtcccact tcattgcgtt tttcagcgat
     2041 tccacggcaa agccgacctt gggcttgtcc gcttcggtgg tgtaaaactc gattttgacg
     2101 tttctgccgc tcatggtggt gaaatagtct tccgtgaccg ccaaatcgcc cgcgaccaaa
     2161 gcaaacagat aactcggttt ggcaaacggg tcttcccatt tcacccaatg gcggccgtct
     2221 gaatactcgc cgccgtcgat tttgttgccg ttggagagca aaacgggata gcgttttttg
     2281 tccgcgacga tggtggtcgt gaacttggac atgacatccg gacggtcgat atagaacgtg
     2341 attttgcgga agccctccgg ctcgcactgg gtaaacaggt taccggcgga cgcatacagc
     2401 cccatcagcg atttgttttc cgccggcagg atttcggttt ccacttcgac ggtgaagcgt
     2461 tcggacggca cgtctgcaat cgtcagcgtc tcaccttcca acacataatc cgccgccgcc
     2521 ccgttgattt tgacggacaa gagtttcgcc gaaccgtcca acaccagcgg ctcccctacc
     2581 ctctgcggct caaccgtcaa acgcgacttc acgacggttt gcggttcggc aatatcaaaa
     2641 tgcaggtcgg tttcaaggat gcggtaggca ggcgtttggt aatctttcag ataacggacg
     2701 attttgctca tttttttctt tcaatattgt ttggtttgac tggaaaaggc ttcagacggc
     2761 ataggcaaac cctgcaccga tatgccgtat gaaaccgaaa catcagtgcg gacgcaccgt
     2821 caaacaaccg gttttaattt caatctttat ttccacgctt ggacaaattc tgcccaatgc
     2881 ggtttgtctt ctgtttgggc ggaaagatag tttctgatcc gcttgatttc catttcgtat
     2941 tcgtccgcgt cgaatctgcc gctgaccaga cagaaacgca gatacatcaa atacgtattc
     3001 acggcatcgg tttcacaata attgcggatt tccttcagcc tgcccgcgtg gaacgcatcc
     3061 cacaccttgc tgccgtccat acccagcttg cctggaaaac cgcacagttt cgccatatcg
     3121 tccagcggca cgtttgccct cggctggtaa agcgcgagca aatccatcaa atcgcaatga
     3181 cgttggtggt agcggctgat gtagttgttc cacttgaaat cgcggctgtc gccgaaatcg
     3241 ccgtcaccca tatcccaata gcgcgcggcg ttgatgccgt gtagcagcga acggtaatgc
     3301 agaaccggca ggtcgaaacc gccgccgttc caactgacca gttgcggcgt atgtttttca
     3361 atcaactcga aaaatttggc aatcacgact tcttcgccat cgtccatctc gccgattgta
     3421 ccgacatgga ctttatcctg cccccaacgc atgcagcacg aaatcgccac aatctgatga
     3481 agatgatgct gcataaaatc cccacccgtc tgagcacggc gtttttgctg ggcaaacagc
     3541 accacttcat cgtcgggcag cgaggacggc agctcgtaca atgttcggat accctgcaca
     3601 tcgggtacgg tttcaatatc gaaagccaaa atcgtggtca tgacagcacc ttgtatttaa
     3661 aacagacttg cgcctattgt gtcattaaaa ggcggataaa aaaagaggca accccccaca
     3721 ggattgcccc aatacctcaa atcagagatt tacgcttcac aaacaataca ggctttcgcc
     3781 tgcggctttg cccgcgtagc tcaactctac gccggcaaac ttttgtttca ccgtttccga
     3841 tgaaaccccg accaatcgca agactgaccg gaaaatcctt tcagacggca tttcctgcct
     3901 gtcgtgtaat tccatgtagc gaaatgtacg ccattttcta cgctttgcca agcatttttt
     3961 acaatataaa tgtcaaaaca ttaattttat aaaattgctg aaaatattaa atatatggat
     4021 ttttattttt tatatttcaa taaatataaa tttaattttg atttatattt aaatttaagc
     4081 ataaaatgtc aaatattaaa ggaaacatga aaggcatata ttaaatattt atttataacg
     4141 ctatgttttt aaagaaaatt aattttaata tattaactag attgtctgca tatattcata
     4201 ggtttgcggt atttcttcca aaacctgctt cgaatttccc gaccaagtct taaaaatatt
     4261 gttttttgag atacttaaat agcagcgatt atcaaatgaa atctgttcat ataatctgcc
     4321 attttgcatt taaaaaaaca atcaggagtt tcgactcgaa acgcctgata tgttttgtaa
     4381 ttttacgtag tcagtaaaaa tcggggctgc cttccggacg ggttttaaaa cgcttgtgca
     4441 gccaaaaata ttgttccgga tgttcgcgca ccctgtcttc gataaaacgg ttcatgcgct
     4501 gcgcgtcggc tttcgcgtct tcacccggaa aggatttcca agcagggtag aaatgcaatg
     4561 taaccgtatt gtctgcctcg cggacgggaa tggcgggtat cacttttgca tttgcaagcg
     4621 cggcaatgcg gctcaatccg gtaatcgttg ccgtctgaat accgaaaaaa tccacaaaaa
     4681 ccgaatcgtt gcgtccgaaa tcctgatcgg gcagatacag aaacggcgcg ctgcctttgc
     4741 ggaactgttt gacgagggcg cgcagccctt cggtgcgccc gataaggaaa acgttgtgat
     4801 aacggttgcg gcctttcaaa atctgttcgt ccaatatctt gtttttttgg tgggaataca
     4861 tactaattag cggaacatcc tgattaagcg cgtacaccgc catctcgaac gcggtaaagt
     4921 gcggatacag gatgatgact ttttcccccg ccgccagcgc gtcgtccaaa taatgcttat
     4981 tgcggtaacg caccagcgat ttcaggcgtt tggcaggcgc ataccaatat aagccgtatt
     5041 caagcatcag tttcgccata tgtttgaaat gctgcttcaa tacggtttcg cgcttttttc
     5101 cgtcccactc gggaaagcat tttgccaaat tgatttcgcc gatacggcgg cggggtttga
     5161 ccaaaaggta ggcgagcaaa cccgtcaggt cggcaagttt gtgcagcagc gcaaacggca
     5221 gaaactgcaa aacatacagt acaaaaaata taaatttcat ctcgatacac attttctttt
     5281 cagacggcaa aatacaaatg ccgtctgaaa ctattgaaac ctgccgcgct tgacctgcat
     5341 ccccgaagga ttgagtttgg cggcaagccc gtggttgcgt aaggcgtggg tcagcgcgac
     5401 ggcaagaccg tccgccgcat ccgcctgcgg cgttcccgat aagccgagca tctgcaccac
     5461 catatgctgc acctgttctt ttgccgcctt gcccttgccg actaccgcct gtttgacctg
     5521 caaggccgtg tattccgaaa cgggcagctt atggctgacc aatgccgcca atgccgcgcc
     5581 cctagcctga ccgagcatca gcgtcgatgc cggattgacg ttgacgaaca cctgttccac
     5641 tgccgcctgt tgaggcttgt aaacggtaac gacttcgccg atgtgccgga cgatgacggc
     5701 aatcctgtct gccagaggcg catcggcagg cgttttgatg cagcctgagg caacgtaaaa
     5761 atgatcgcgc cccctgacat cgatgatgcc gaaacccgtt acgcggctgc ccgggtcaat
     5821 gcccaagata cggacggttg ccgacatatt cacagcaaac cgtgttgaat cagtttttta
     5881 cgcaaggtat tgcggttcag tcccaacatg acggaggctt tggactggtt gccgccgcat
     5941 tgctccatca cgcacaccag cagcggtttt tccacctgat gcaataccat atcgtacacg
     6001 ccgcaaggtt cggtaccgtt caggtctttg aaatattgtt ctaaattttg tctgatgcat
     6061 tgggaaatat cgggaagggt atggggcatg attgcacttt caaaggataa tcaagtgttc
     6121 agaaggcatt tgggcggtag gcgcacgccc aactgtcggt tttttcggca agtctttcaa
     6181 gataacctgc aagcatgtcg tattgcgccg ccgcactgtc caagcggttg atttcacgac
     6241 gtgtctgttc gccgtcgggc atttcgtcga tgtaccagcc tatgtgtttg cgtgcgatgc
     6301 gtacgcctgc actatcgccg taaaacgcgt gtatggcgcg gatgtggttc aaaatagcgg
     6361 cggcgcattc tgccaaactc aaggcaggcg gtaaaacacc gtgttcggcg taatgtttca
     6421 aatcgcggaa gaaccacggt ctgccttgcg cgccgcgccc tatcatgatg ccgtcggcgg
     6481 cggtttgttt gaggacggcg gcggcttttt gcggcgaagt gatgtcgccg ttgacccaga
     6541 ccgggatgtt cagacggcat ttggtttcgg caatcaggtc gtaagccgcc tcgcctttgt
     6601 acatttgcgt gcgcgtgcgt ccgtggacgg caagggcggc aatgccgcaa tcttcggcga
     6661 ttttggcgat gacgggcagg tttttgtggt cgtcgtgcca acccaaacgg gttttgaggg
     6721 taacgggtac acctgccgca cggacgacgg cttccaaaat ggcggcaacc agcggctcgt
     6781 tctgcatcag cgcgctaccg gcttggacat tgcagacttt tttagcggga cagcccatgt
     6841 tgatgtcgat aagctgcgcc ccgaggctga cgttgtaacg cgcggcatca gccatctgct
     6901 gcggatcgct gccggcaatc tgcacggcaa cgacaccgcc ttcatcggca aaatcgctgc
     6961 ggtgcagggt ttttttggta tttctgagcg tcgggtcgct gatcagcatt tcgcacaccg
     7021 cccaacccgc gccgaaatcg cggcagaggc ggcggaaggg tttgtcggta atgcccgcca
     7081 tcggtgcaag tgcgatgggg ttgtcgataa aatagccgcc gatgtgcata atgggcccgc
     7141 gtttcaaaaa agtgcgacat tgtacatttt ttaagcagga tttccaatct ccggacgcgc
     7201 ccgcgattgg ggcgggcacc gttttatggc ataatccgca cacagatttc ctgccccgcc
     7261 actcacaggc gg
//
LOCUS       DAWFAN010000096         7264 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWFAN010000096 DAWFAN010000000
VERSION     DAWFAN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.096"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..821
                     /gene="lpt3"
                     /locus_tag="WGO65_001409"
     CDS             <1..821
                     /gene="lpt3"
                     /locus_tag="WGO65_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ0327698.1"
                     /translation="VKQSYSAGFMTAVSLPSFFNVIPHANGLEQISGGDTNMFRLAKE
                     QGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLGYGNGDNMPDEKLLPLFDKINL
                     QQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDKYDNTIHKTDQMIQTVFEQLQK
                     QPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPLVLYSPNKAVQQAANQAFAPCE
                     IAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGDAGSLSIRDGKAEYVYPQ"
     gene            887..4201
                     /locus_tag="WGO65_001410"
     CDS             887..4201
                     /locus_tag="WGO65_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ0327699.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMCEWRELHHQLAQT
                     AIEMGLTTKETAFRRPPEIRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRTAKEAG
                     YEQIHRALLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TKLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYNERLPEMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSR
                     QNENHGNTVGWVENPTSAATAKTVGFDNPTYATQQPTPSLAGEGRGEGKTVAAQTNFS
                     ATSVSPLPNPLPQEREQSAAASTVSDGLHPANSQQTAPSLVGEGWGEGKTVAAQTNFS
                     ATSASPLPNPLPQERKQSAAASKVSDDPKPKKRPASPKGRLKPLPLADIRTFEAWLKT
                     AERDNPRLLFLSRDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLT
                     LPLTVLNRLHAPSLEWLVPGMLREKIQLLIKALPKQIRRICVPVPDFITKFLESNPDR
                     QAAIIPQLAHFIAKSAGDMRILEQIDQDAWTAQELPEHCYLNLRIIDDGGQELAGGRK
                     LHELQQQLGQAAAVTFRDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKE
                     KDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINA
                     DTLCDDLTQAVCDRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAY
                     AELNGKLGKHPLTHLLKLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNP
                     ARDAAREADIQELEQMWQEKTDSLIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPY
                     PVSVKRLLKEWERLNK"
     gene            4260..5867
                     /locus_tag="WGO65_001411"
     CDS             4260..5867
                     /locus_tag="WGO65_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0327700.1"
                     /translation="MKSENNYERIVQNSKKFQHSQKNEMQKRITDLLQQLTNGIHERD
                     QIMAVSLLGAIAGHNTFLFGPPGTAKSLISRRLACAFENPQYFEYLMNRFSTPEEVFG
                     PVSIKALKEDRYLRKIEGYLPTADFAFLDEIWKSSPAILNNLLTIINEHIFKNGDERF
                     NVPLKALIAASNEIPPENQGLEALYDRFIIRLLVPPIEQEENFNRLLDSKPSSDKPEI
                     SEDLRISYHELAKWREQLHKVRLSTDTLLIIKYIREELAAQFDKLGVYVSDRRWQRAA
                     VLLKASAFCNGRKETNHSDVILLKYCLWTTPENRESVEQIVMNTIKSCGFDTGIDLAE
                     LDREKESLDKEINKELYHSQDVYETVEMSGKKYFPVQAVFKNVHNSHANKELKIYVPY
                     SKFRSKEKFYPIDSTGNEIQEIECEFDGQGCCKLELKVRGGHWNEQYKPFKFKPNVLF
                     HKGDKKQSINDRLIWSLSRSVMEINKALLDVLKLVTKKHAEHQQFLQSPFITQDDIDI
                     AVSGILDQIDKLNLRIKDCERLESLCR"
     gene            5858..>7264
                     /locus_tag="WGO65_001412"
     CDS             5858..>7264
                     /locus_tag="WGO65_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEZ0327701.1"
                     /translation="MSLNSKLSEQIRWIKASLQIDYADLFKQHGSLNGQIQQRLSDWQ
                     YQTKLALLQKNPFVLHEEKLKKHQQTEIFNQQSVDDYRYFCKLAGVPFEKEFWQQELT
                     QSKNSKPDKQNLPLKLLTEKWQQQLDYAKSQWYLEQLNQLRQEFIDKLKQELDTIRQL
                     AEQLEQFGFEAGLWFDNSIGNLSQQNIEEMKRWLNYLTQDKNAQQIAELLGKMRQIEQ
                     SEKIEQVKQTVYTKSPQIDVNSREEIIGLRLGKDLEYVLPSELALMADEETSTLFDLK
                     FLESKLMCFELQGMTYCDAPTEIIVEQKSQEDEKLGPMILCVDTSGSMVGLPENIAKA
                     MALFLGAKAKSENRSCFVINFSTGIETFELTSKTGISNLIAFLRQSFHGGTDAAPALR
                     HALKMMEQEIYQKADLLMISDFVMNSLPDDLLASIEIQRETGNQFNSLVIGDAFMSKR
                     LKTHFDREWIYNPNVQTIQ"
BASE COUNT         2201 a         1791 c         1634 g         1638 t
ORIGIN      
        1 ttgtgaaaca aagttattcc gcaggcttta tgacggcagt atccctgccc agtttcttta
       61 acgtcatacc gcatgccaac ggcttggaac aaatcagcgg cggcgatacc aatatgttcc
      121 gcctcgccaa agagcagggc tatgagacgt atttttacag tgctcaggct gaaaaccaaa
      181 tggcaatttt gaacttaatc ggtaagaaat ggatagacca tctgattcaa ccgacgcagc
      241 ttggctacgg caacggcgac aatatgcccg atgagaagct gctgccgctg ttcgacaaaa
      301 tcaatttgca gcagggcagg cattttatcg tgttgcacca acgcggttcg cacgccccat
      361 acggcgcatt gttgcagcct caagataaag tattcggcga agccgatatt gtggataagt
      421 acgacaacac catccacaaa accgaccaaa tgattcaaac cgtattcgag cagctgcaaa
      481 agcagcctga cggcaactgg ctgtttgcct atacctccga tcatggccag tatgtgcgcc
      541 aagatatcta caatcaaggc acggtgcagc ccgacagcta tattgtgccg ctggtgttgt
      601 acagcccgaa taaggccgtg caacaggctg ccaaccaggc ttttgcgcct tgcgagattg
      661 ccttccatca gcagctttca acgttcctga ttcacacgtt gggctacgat atgccggttt
      721 caggttgtcg cgaaggctcg gtaacgggca acctgattac gggtgatgca ggcagcttga
      781 gcattcgcga tggcaaggcg gaatatgttt atccgcaata agcgacgtga aaaccaataa
      841 aaacaaattt agatgatgtc ggggaagatg cccgaccgac aagactatgc aaaatatgaa
      901 aaaccaagta cgcggatcag gcatggatgc acgatccaat ccggccaatg tttcagacgg
      961 cctgcaaaac agttcgggtc atatcggtac caacacgcgt taccgcctga ccaaactcgg
     1021 cgaacaaatg gcgcgcctgc ccatcgaccc gaaaatcgcg cgcattttgt tggcggcgaa
     1081 gaaacacgac tgcatggcgg aaatattggt gatcgcgtcc gcactgtcga ttcaagaccc
     1141 gcgcgagcgg ccgctggaag cgcgtgatgc cgccgccaag gcgcacgagc gttttaccga
     1201 caagcaatct gactttcttg cctatctgaa tatttgggac agctttcagc gcgagcgcga
     1261 caaaggtttg tccaacaagc agctggtgca gtggtgccgc caatattttc tgtcgcacct
     1321 gcgtatgtgc gaatggcgcg agctgcacca ccagcttgcc caaaccgcga ttgaaatggg
     1381 tttgaccacc aaggaaaccg ctttcagacg acctcccgaa atcaggcagc tcacgtcgtc
     1441 tgaaaatgcg ggtgaccaag acttatccgc caagctcaaa caaaaacaac tggataaaaa
     1501 gcaacaccgc gcccaaatcc gcaccgccaa agaagcgggc tacgaacaaa tccaccgcgc
     1561 cctgctgacc ggccttatcg ccaacgtcgg catgaaatcg cccgacggca acgactacac
     1621 cggcgcgcgc ggcagccgct tccacctttt ccccgcctcc gccctgttca aagccaaacc
     1681 caaatgggtg atggcggcag aactcgttga aacgacaaaa ctttatgcgc gcgacgtcgc
     1741 cgttatccag cccgaatgga tagagcagga agccccgcac ctcgtccgct accactattt
     1801 cgagccgcac tgggaacaaa aacgcggcga agtcgtcgcc agcgaacgcg tgacgcttta
     1861 cggtctgacc gtattgccgc gccgccccgt gtcttacggc aaagttgccc ccgaagaagc
     1921 gcgcgaaatc tttatccgca gcgcgttggt ggcgcaggaa tgcgatttga aagcggattt
     1981 ttttgtccac aacaaaaagc tgattaaaga aattaccgaa ctcgaacaca aatcgcgcaa
     2041 gcaagacgtg ctggtcgatg acgaagccct gtttgcgttt tataacgaac gactgcccga
     2101 aatggcttgg aaagacgcgc aaggcagcgt ttggggaagt gaagattccg tacggattat
     2161 tgaatctgac aaagccgaga ggtcgtctga aaatgagcgc aacgagtttc gtaaaaacaa
     2221 gcgtaatggg tctcgccaaa atgaaaatca cggcaacacc gtaggttggg ttgaaaaccc
     2281 aacatcagcc gcaactgcaa aaactgttgg gtttgacaac ccaacctatg ctactcagca
     2341 acccaccccc tccctcgcgg gggagggtcg gggagagggc aaaacagttg ccgctcaaac
     2401 taacttttct gcaacttcag taagccctct ccctaaccct ctcccacagg agagggaaca
     2461 gagtgccgcc gcttcaacgg tttcagacgg cctgcatcct gcaaattccc agcaaaccgc
     2521 cccctccctc gtgggggagg gctggggaga gggcaaaaca gttgccgctc aaactaactt
     2581 ttctgcaact tcagcaagcc ctctccctaa ccctctccca caggagagga aacagagtgc
     2641 cgcagcttca aaagtttcag acgaccccaa acccaaaaag cggccggcat ccccaaaagg
     2701 ccgtctgaaa cctttgcccc ttgccgatat ccgcaccttt gaagcctggc tcaaaaccgc
     2761 cgagcgcgac aatccgcgtc tgctgttcct cagccgcgac gatctgatgc aacacgccgc
     2821 cgcgcacatt accgaagagc agttccccaa attttggcaa accgcagacg gcaagttcaa
     2881 actttcctac cgcttcgagc cgcaccatcc gctcgacggc gttaccctca ccctaccgct
     2941 gaccgtcctc aaccgcctgc acgcgccgtc gctcgaatgg ctggtgcccg gtatgttgcg
     3001 cgaaaaaatc cagctgctga ttaaagccct gccgaagcaa atccgccgca tttgcgtgcc
     3061 cgtacccgat ttcatcacca aattcctcga aagcaatccc gaccgccaag cggccatcat
     3121 tccccagttg gcgcacttta ttgccaaaag cgcaggcgat atgcggattc tcgagcaaat
     3181 cgaccaagac gcatggacgg cgcaagaatt gcccgaacac tgctatctga atctgcgcat
     3241 tatcgacgac ggaggacaag agcttgccgg cggccgcaaa ctgcatgaat tgcaacaaca
     3301 actcggccaa gccgccgccg ttaccttccg cgacaacacc caagaattcg agcgcgacaa
     3361 cgtgaccaca tgggacattg gcaccctgcc cgaatccatc aaattcgcac gcggcaaaca
     3421 acagctcacc ggctacctcg gtctgcaaaa agaaaaagac ggccgcatcg ccctgcgcct
     3481 gttcgacacc accgaagccg ccgagcaggc acaccgtcaa ggcgtgatcg aattgatgaa
     3541 actgcaatta aaagagcagg taaaagattt gaacaaaggc atccaaggct tcacccaagc
     3601 cgccatgctg ctcaaacaca tcaacgccga caccctgtgc gacgacctca cccaagccgt
     3661 ctgcgaccgc gcctttatcg gcgaagacga gctgccgcgc aacgaaaaag ccttcaaaga
     3721 acaaatcaaa cgcgcccgca gccgcctgcc cgccgtcaaa gaagccctca gccgctactt
     3781 gcaggaaacc gccgccgcct acgctgaact caacggcaaa ctcggcaaac acccgctcac
     3841 ccatctccta aaactacgcc tgcaaaccct gctcgccgcc ggcttcgcca cccgcacccc
     3901 gtgggcacaa tggccgcgcc tccccatcta cctcaaagcc atgaccctgc gcctcgaaaa
     3961 atacagcagc aaccccgccc gcgacgcagc ccgcgaagcc gatatccaag agctggaaca
     4021 aatgtggcag gaaaaaacag acagcctgat taaacaaggt ctccccattt cagacggcct
     4081 cgccgcgttt aaatggatga ttgaagaatt gagggtgtcg ctgttcgcgc aggaattgaa
     4141 gacaccgtat ccggtgtcgg tgaagcggtt gttgaaggag tgggagaggt tgaataagta
     4201 gtgatattat ccaatacatt tacaggaaaa tttctaactt acataaaagg tacaactcaa
     4261 tgaaaagcga aaataattat gaacgtattg ttcaaaattc taaaaaattc caacactctc
     4321 agaagaatga gatgcagaaa cgcataacag atttgctgca acaactcaca aatggcattc
     4381 atgaacgcga ccaaattatg gcggtttccc tacttggtgc gattgccgga cataatactt
     4441 tcctatttgg accgccggga accgcgaaaa gtctgatttc ccgccgtttg gcatgtgcat
     4501 ttgaaaatcc gcaatacttt gaatacttga tgaaccgttt cagcacacct gaagaagtat
     4561 tcggacctgt atctattaag gcattgaaag aagaccgata tctccgaaaa atcgaaggct
     4621 atttgccaac tgccgatttt gcatttttgg atgaaatttg gaaatccagt cctgcaattt
     4681 tgaataacct gctgaccatt atcaacgagc atatttttaa aaacggcgat gagaggttta
     4741 atgtgccact taaagccctg attgcggctt ccaacgaaat tccacctgaa aatcaaggtt
     4801 tagaggcact gtatgaccgc tttattatcc gtctgcttgt gccgccgatt gaacaagaag
     4861 agaacttcaa ccgtttgctg gatagcaagc caagcagcga caaacctgaa atcagtgaag
     4921 atttacggat tagttatcat gagcttgcca aatggcgtga acaactccac aaagtcaggc
     4981 taagtacaga tacattactg attattaagt atatcagaga agaattagca gcccagtttg
     5041 ataagttagg cgtttatgta tccgaccgac gttggcaacg tgcagccgta ttattaaagg
     5101 cttctgcttt ttgcaatgga cgcaaagaaa ccaatcatag cgatgtgatc ttgctgaaat
     5161 attgtctgtg gacaacacct gaaaatcgag agtcagtgga acaaattgtg atgaatacca
     5221 ttaaatcctg tgggtttgat acaggtattg atctggctga attagataga gaaaaagaga
     5281 gtttggataa ggaaataaat aaggaacttt atcattcaca agatgtttat gagacggttg
     5341 aaatgagtgg aaaaaaatat tttccagtac aggctgtttt caaaaacgta cataatagtc
     5401 atgctaataa agaacttaag atttatgttc catatagcaa atttcgtagt aaagaaaaat
     5461 tttatccaat agatagtaca ggaaatgaaa ttcaagaaat tgaatgtgaa tttgatgggc
     5521 aaggttgctg taaattggaa ttgaaagtgc ggggtggtca ctggaatgag caatataagc
     5581 cattcaaatt taaacctaac gttttattcc ataagggaga taaaaaacaa agcatcaatg
     5641 ataggttgat ttggtcttta tcacgttctg ttatggaaat caataaagca ttattagatg
     5701 tattgaaatt agtcaccaaa aaacatgcag aacatcagca attcctacaa agccctttta
     5761 ttacccaaga tgatattgat attgcggtat caggaatttt agaccaaatc gacaaactca
     5821 atttacggat taaagactgc gaacgtttgg agtctttatg tcgttaaata gcaaattatc
     5881 cgaacaaata agatggatta aagcttctct acaaatagat tatgcagatt tatttaaaca
     5941 gcatggttcg ttaaatggac agattcagca acgtttatca gattggcaat atcagaccaa
     6001 actggctttg ttgcagaaaa acccgtttgt attacatgaa gaaaagctaa aaaaacacca
     6061 acaaacagaa atttttaatc aacagtctgt tgatgattat cggtattttt gcaaattagc
     6121 aggcgtgcca tttgaaaaag aattctggca gcaggaatta acacaaagca aaaattcaaa
     6181 acccgataaa cagaatttgc ctttgaaatt gctaacagaa aaatggcagc agcaattaga
     6241 ctatgccaaa tctcaatggt atttagagca gcttaatcaa ttacgacagg aatttataga
     6301 taagttaaaa caggaactcg acactatccg acagcttgca gaacagttag agcaatttgg
     6361 ttttgaagca gggctgtggt ttgataacag tatcggcaat ttaagccagc aaaatattga
     6421 agaaatgaaa cgctggctga attacttgac ccaagataag aatgcccaac agattgctga
     6481 attactaggc aaaatgcgcc aaatcgaaca atctgaaaaa atcgaacagg taaaacagac
     6541 tgtttacaca aaaagcccac aaattgatgt gaattcgcgt gaagaaatta tcggattacg
     6601 tttaggtaaa gatttagagt atgtattgcc gtctgaattg gctttaatgg cagatgaaga
     6661 aacatcgact ttatttgatt tgaagttttt ggaatccaaa ttaatgtgtt ttgaattgca
     6721 aggaatgacc tattgcgatg ccccaactga aattatagtt gaacaaaaaa gccaagagga
     6781 tgaaaagctc ggaccaatga ttttatgtgt agataccagt ggttcaatgg ttggtctacc
     6841 tgaaaatatt gccaaagcaa tggctttatt tttaggagca aaagctaaaa gcgaaaaccg
     6901 ttcttgtttt gttatcaatt tttccactgg aatagaaact tttgaattaa caagtaaaac
     6961 agggatttcc aatttgattg cttttttacg tcaatccttt catggtggta cagatgctgc
     7021 cccagcttta cgtcatgcgc taaaaatgat ggagcaagaa atctatcaaa aggcagactt
     7081 attaatgatt tccgattttg tgatgaacag cttacctgat gatttgttgg catcaatcga
     7141 aatacaacga gaaaccggta atcagtttaa ttctttggtt attggtgatg cttttatgag
     7201 taaacgccta aaaacacatt ttgatcgtga gtggatatac aatcctaatg ttcagacaat
     7261 tcaa
//
LOCUS       DAWFAN010000097         7230 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWFAN010000097 DAWFAN010000000
VERSION     DAWFAN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.097"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(36..521)
                     /gene="purE"
                     /locus_tag="WGO65_001413"
     CDS             complement(36..521)
                     /gene="purE"
                     /locus_tag="WGO65_001413"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ0327702.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEACVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(561..1277)
                     /locus_tag="WGO65_001414"
     CDS             complement(561..1277)
                     /locus_tag="WGO65_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ0327703.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGRIVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFAAAAALLKGADGGDGGSIAQRSMYL
                     LLQSRVRMGNCESVIEIGRRYANRFKGTPAAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQTYPGSPAAKRAAAAVRKR"
     gene            complement(1277..1945)
                     /locus_tag="WGO65_001415"
     CDS             complement(1277..1945)
                     /locus_tag="WGO65_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ0327704.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPMPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(1956..2141)
                     /locus_tag="WGO65_001416"
     CDS             complement(1956..2141)
                     /locus_tag="WGO65_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ0327705.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            2284..4260
                     /gene="mutL"
                     /locus_tag="WGO65_001417"
     CDS             2284..4260
                     /gene="mutL"
                     /locus_tag="WGO65_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ0327706.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELDGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSDNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFNRASDYPTGNKPDTRNAFGASGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDADLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSPSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGKLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLNELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4355..6484
                     /gene="dnaX"
                     /locus_tag="WGO65_001418"
     CDS             4355..6484
                     /gene="dnaX"
                     /locus_tag="WGO65_001418"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ0327707.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAATSCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPETETAQTSVQTASAAAMPSESKTAEPVSNQENNDVPPWEDAPDEAQTAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSETPSENPIQATPNNEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDNAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWQDEAGRETPAMQDKRVQIEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5544..5583
                     /gene="dnaX"
                     /locus_tag="WGO65_001418"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6565..6900
                     /locus_tag="WGO65_001419"
     CDS             6565..6900
                     /locus_tag="WGO65_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ0327708.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFTQGL
                     PPGVGDFFR"
BASE COUNT         1786 a         2216 c         1830 g         1398 t
ORIGIN      
        1 aatttaagcc actatatatt cgcggacggc gggttttaaa tctgttccaa ttccatactc
       61 aaaacagcct gttcctgttt ggctcggaag tctgccagtt tttgcgccag ttcgggggtt
      121 tcgttggcga gcatggaaac ggcgaacaat gcggcatttg ccgcgcctgc ctcgccgatg
      181 gcgaatgtgg cgacgggtac gcctttgggc atttgtacaa tcgataaaag cgaatcttcg
      241 ccgcgcaggt atttgctggg gacgggtacg cccaaaacgg ggacggtggt cttggcggca
      301 accataccgg gtaaatgcgc cgcgccgccc gcacccgcga tgatggcttt gatgccgcgc
      361 gcccgtgcgg tttcggcgta ttggaacatt aaatccgggg tgcggtgtgc ggaaacgacg
      421 cacgcctcat attctacgcc gaactcttca agaaactgcg ctgcctgccg cataacgggc
      481 caatcgctgt tgctgcccat gatgatgccg atttgaatca taaatcctcc ttggtgcgga
      541 tggggtaaaa agcggaaaaa ctatcgtttg cgtacggctg ctgcggcgcg ttttgccgcc
      601 gggctgccgg gataggtctg tatcaggctg cgccaagtcg cccttgcaat gtctttttgc
      661 tgcaacctgt attggcattc gccgattttg aatatggctt cgggtgcggc aggggtgcct
      721 ttgaaacggt tggcgtaacg ccgcccgatt tcgatgacgg attcgcagtt ccccatacgc
      781 accctgcttt gcagcaacag gtacatactg cgttgcgcga tgctgccgcc gtcgcctccg
      841 tccgcgcctt tcaacagggc ggctgcagcg gcgaacctgc cgcttttata gtgtttgagt
      901 gcctgattgt agaggttttg tgcggtttcg acggtatgtg cggatgcgct gccgccttcg
      961 gtattgaggt aatgctcttt caacttgcgg tcgtcgagtt tttggacgta tgccctgctg
     1021 gaagggtgtg ttttcgcatg ctccagtgct ttgactttgc cgtttaaggt ttccacttcg
     1081 ttcgacagcc ggacgattct gccttccaga tagtccaaac ggtcttgcaa ggtcggaacg
     1141 ggatagggaa tagcgtccga actacctgcc tctgcctgcg gttcgagccg catatcctga
     1201 atattgcggg aaacagggga agaacaggcg gcggatacgg acagccaaat gataaaaagc
     1261 ggtaatttgg tcttcattat tttttcagaa gcagggtcaa accgtcgccg acgggcaggg
     1321 tgatggggac aatccgcgta tcgttcggca ggttttgatt aaaatctttg aggatgccga
     1381 cgctgggcgg cgcatcggaa gccgcttcgc gcatcaccct tccgttcaac aaaatattgt
     1441 cgatggcaat gatgccgcct tgccggacaa gcttgaggca acgctcgaaa tattgcggca
     1501 tgggcggttt gtctgcgtct atcagtgcca aatcgtagct tccggcttca ccctgtgcaa
     1561 tcaaatcatc caatgtcagc aatgcgggtt gcaggtgcag gctgatttta tgtgccacac
     1621 cggcctcgtt ccaaacctga cgcgccgtat cggtaaaggt tacattgatg tcgcaggcgg
     1681 taatccgccc gtgttcgggc aacgccaatg caagcgcggt gctgctgtat ccggtaaata
     1741 cgccgatttc cagatatttt tccgcacgga tcagctttgc cagccaaacc aaaaccgccg
     1801 cctgttcgcg cgcaatcgcc attttgccca tacggtgatg cccggtcttc tcacgcagcc
     1861 gcgtcaaaac gggatgttcg ggttcgccga tggcgttcaa atagttttgc aggtccggtg
     1921 caacattgga cagatgggtc gtcatttcgg cgggttcagt cttggtaata ggtataaggt
     1981 tttttcgcta cttttgccgc ctcgaagttt tcctgttctt cgggattgag ttcgacatcc
     2041 cacaaaagcc cccggttttc caaacgctgc tgttccaact caggtttttc ttcaatcagg
     2101 cggttgagga attgtgtggc ttcggattgg tagtgataca tctttgcgct ccaattttac
     2161 gggatacggc gtgattatac tggtattttc caaacgggat aaacggcttt tatcaagaat
     2221 acaggcagaa agataagggg ttttattata gaataaagcg tttttgcaac ggaagcctac
     2281 cttatgccac gcattgccgc cctgcccgac catctcgtca accaaatcgc cgccggcgaa
     2341 gtagtcgaac gccctgccaa cgccttgaaa gaaatcgttg aaaacagtat cgatgcaggc
     2401 gcaacggcga ttgatgtcga gctggacggc ggcggcatcc gcctgattcg cgtcagcgac
     2461 aacggcagcg gtatccatcc ggacgacatc gaactcgcgc tccaccgcca cgccaccagc
     2521 aaaatcaaaa ccttaaatga cttggaacac gtcgccagca tgggctttcg cggcgaaggc
     2581 ttggcaagca tcgcctccgt cagccgcctg accctgacca gccgtcaaga agacagttcg
     2641 cacgcaaccc aagtcaaagc cgaagacggc aaactcagca gccccaccgc cgccgcccac
     2701 cccgtcggca ccaccatcga agccgccgaa ctcttcttca atacccctgc gcggcgcaag
     2761 ttcctcaagt ctgaagccac cgaatacgcc cactgcgcca ctatgctcga acgcctcgcg
     2821 ctggcgcatc cgcacattgc cttctcgctc aaacgcgacg gcaaacaagt gttcaaactc
     2881 cctgcacaaa gcctgcatga acggattgcc gccattgtcg gcgatgattt tcagacggca
     2941 tcattggaaa ttgacagcga caacggcgca ttgcggctct atggcgcgat tgccaaaccg
     3001 actttcgcca aaggtaaaac cgacaaacaa tactgcttcg tcaaccatcg ttttgtccgc
     3061 gacaaagtca tgctccatgc cgtcaagcag gcataccgcg acgtattgca caacgcactg
     3121 acgcctgctt tcgtcctctt tctcgacctg ccgcccgaag ccgtggatgt caacgtccac
     3181 ccgaccaaaa ccgaaatccg cttccgcgac agtcagcagg tgcaccaact tgtgttccac
     3241 acgctcaaca aagcccttgc cgacacacgc gccgacctga ccgaaagcgt cagcaacgca
     3301 ggcgaagtgt tgcatgacat taccggcgtt acgcctgcac caatgccgtc tgaaaacgac
     3361 agcgaaaatc tgtttaatcg cgcatccgac tacccgacag gcaacaaacc cgatacgcgc
     3421 aatgcctttg gtgcatcagg caaaaccgcg cctatgccct atcagtccgc atatgcgccg
     3481 caacaacgca gcctgtccct gcgcgaaagc cgcgcggcaa tgaacaccta tgccgaactc
     3541 tacaaaaaaa ccgacgatgc cgaccttgag ttaagccgat tcgagcaggc acgtttcggc
     3601 aatatgccgt ctgaaacgcc tgctccccaa acagatacgc cgctttcaga cggcatcccg
     3661 tccccatccg aactgccgcc gctcggtttc gccattgccc aattgctagg catctacatc
     3721 cttgcccaag ccgaagacag cctgctgctc atcgatatgc acgccgctgc cgaacgcgtc
     3781 aactacgaga aaatgaaacg ccaacggcag gaaaacggca agctgcaaag ccaacgcctg
     3841 cttattcccg taacctttgc cgcgtcccac gaagaatgcg ccgccctcgc cgattatgcc
     3901 gaaacgctgg caggcttcgg cttggaattg tccgatatgg gcggcaacac cctcgccgtc
     3961 cgcgccgttc ccgccatgct cggcaaagcc gatgtcgtct ccctcgccaa agacgtatta
     4021 aacgaactcg cccaagtcgg cagcagccaa accatcgagg aacacgaaaa ccgcatcctc
     4081 gccacgatgt cctgccacgg ctcgatccgc gccggccgcc ggcttaccct gcccgaaatg
     4141 aatgcgctcc tgcgcgatat ggaaaacacg ccgcgcagca accagtgcaa ccacggccgt
     4201 ccgacttggg tcaaactgac tttgaaagaa ttggacgcac tgttcttgcg cggacaataa
     4261 gccgaaagtg ctagaatacg ccgcccgaga ccgccgttca gacggcattc cgacgcaccg
     4321 acagaaacat cacgaccgaa accaagagaa aaatatggcc tatcaagttc tcgcccgaaa
     4381 atggcggccc aaaacctttt ccgacctagt cggtcaggaa cacgtcgtca aagccctgca
     4441 aaatgccttg gacgaaggca ggctgcacca cgcctacctg ctgaccggta cgcgcggcgt
     4501 gggcaaaacc accatcgccc gcatccttgc caaaagcctc aactgcgaaa acgcgcaaca
     4561 cggcgaacct tgcggcgtat gcgaaagctg tacgcagatc gatgccggac gctatgtcga
     4621 cctgctcgaa atcgacgccg cctccaacac aggcatcgac aacatccgcg aagtcttgga
     4681 aaacgcccaa tacgcgccga ccgccggcaa atacaaagtc tatatcatcg acgaagtgca
     4741 tatgctttcc aaaagcgcgt tcaacgctat gctcaaaacg ctggaagagc cgcccgaaca
     4801 cgtcaaattc atccttgcca cgaccgatcc gcacaaagtc cccgttaccg tattaagccg
     4861 ctgcctgcaa ttcgtcttac gcaatatgac cgcgcagcag gttgccgacc accttgccca
     4921 cgtcctcgac agcgaaaaaa tcgcctacgc cccccccgcc ctgcaacttt tgggacgcgc
     4981 cgccgccgga tcgatgcgcg atgccttgag cctgctcgac caagccatcg ccatgggttc
     5041 gggcagcgtt gccgaacaag acgtccgcca aatgatcggt gcggtcgata aacaatacct
     5101 gtacgaactg ctgacaggca tcgtcaacca agacggcgaa gccctgctgg caaaagcaca
     5161 ggaaatggct gcgtgtgccg tcggctttga caacgccttg ggcgaacttg ccatactgct
     5221 gcaacacctc gccctgatac aggcagtgcc gaatgccttg gcgcacgacg accccgattc
     5281 cgatattttg caccgcctcg cccaaaccat aagcggcgaa caaatccagc tttactacca
     5341 aatcgccgtc cacggcaaac gcgacctgag cctcgccccc gacgaatacg ccggctttat
     5401 gatgaccctg ctgcgtatgc tggcgttcgc acccttggcg gcaacatcgt gtgatgcaaa
     5461 cgccgtgatt gaaaataccg aacttcaatc cccatcggca caaaccgccg aaaaggaaac
     5521 cgccgcaaaa aagccccaac cgcgccctga aacggaaacc gcccaaacat ccgttcagac
     5581 ggcatccgca gcggcaatgc cgtctgaaag caaaactgcc gaacccgttt ccaatcaaga
     5641 aaacaacgat gttccgcctt gggaagacgc gccggacgaa gcccaaaccg cagccggcac
     5701 ggcgcaaacg tcggcaaaaa gcattcagac ggcatccgaa gccgaaacgc cgcccgaaaa
     5761 ccaagtttcc aagaacaagg cagccgacaa cgaaaccaat gcccccttgt ccgaaacgcc
     5821 gtctgaaaac cccattcagg caacaccgaa taatgaagcc gttgaaacgg aaacatttgc
     5881 acacgaagcc cctgcagaac ctttctacgg ttacggcttt ccggataatg actgccccgt
     5941 agaagacaac gcagaaatcc cgccgcccga ttgggaacac gccgcccctg ccgataccgc
     6001 aggcggaggc gcggacgaag aagcagaagc gggcggcatc ggcggaaaca acacgccgtc
     6061 cgccccgccg cccgaatttt ccaccgaaaa ctgggcagcc atcgtccggc atttcgcccg
     6121 caaactcggc gcggcgcaaa tgccggcgca acactccgcg tggacggaat accatcccga
     6181 caccggcctg atggttttgg cgatgaccgc cgaagcacgc gccaccgccg acaaaaaacg
     6241 cctcgacaaa atccgcgaca cccttgccca agcctacggg ctgcaactca ccctgcaaac
     6301 ccaagactgg caggacgaag ccggcagaga aacccccgca atgcaggaca agcgcgtcca
     6361 aatcgaagac aggcaaaaag cacaagcatt gctcgaagcc gaccccgccg cacaaaaaat
     6421 cctccaagca ttcggcgcgc aatggcagcc cgaatcactg gaattggcgg caaaccggcc
     6481 ataaacagat ataatgccgc ccgaaccctt cggacggcat tgccgtttcc ctcattcatt
     6541 caaacacaaa caggagtatt cagtatgttc ggaaaagccg gattaggcgg cctgatgaaa
     6601 caggcgcagc aaatgcagga aaatatgaaa aaagcgcaag ccaaacttgc cgaaaccgaa
     6661 atcgaaggcg aagcaggcaa cggcttggtc aaaatcacaa tgacctgcgc gcacgaagta
     6721 cgcaaaatcg acatcagccc cgatttgatt caagaagccg ccgacgacaa agaaatgctc
     6781 gaagacctca tcctcgccgc cctcaaatcc gcccgtgaca aagccgaaga aaccgcaaac
     6841 aaaacaatgg gcgcattcac gcaaggtcta ccccccggag tgggcgactt cttccgctga
     6901 tacccaaccg tcattcccac gcaggcggga atctagaacg tagaatctaa gaaaccgttt
     6961 tactcgataa atttccgtgc cgacgggtct agattcccgc gttcgcggga atgacggcat
     7021 attttttgca tttgatataa agggtcgttt gaattttgtt cagcaagtgc aaagtgttgc
     7081 acataaaagg gcgcaggata gaggcaaagc gggcgtaggt cgggctgtag caactgtatt
     7141 tttcaccccg tcgggcaaaa atatagtgga ttaacaaaaa ccagtacggc gttgcctcgc
     7201 cttagctcaa agagaacgat tctctaaggt
//
LOCUS       DAWFAN010000098         7214 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWFAN010000098 DAWFAN010000000
VERSION     DAWFAN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.098"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(16..771)
                     /locus_tag="WGO65_001420"
     CDS             complement(16..771)
                     /locus_tag="WGO65_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ0327709.1"
                     /translation="MKYVRLFFLGAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPRAGE
                     LKQPVTNVSWFAANAYCTAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(949..2127)
                     /gene="nirK"
                     /locus_tag="WGO65_001421"
     CDS             complement(949..2127)
                     /gene="nirK"
                     /locus_tag="WGO65_001421"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ0327710.1"
                     /translation="MKRQALAAIIASMFALAACGGEQAAQAPAETPAASAEAASSAAQ
                     AAAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYHYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGSIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
     gene            2509..4764
                     /locus_tag="WGO65_001422"
     CDS             2509..4764
                     /locus_tag="WGO65_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ0327711.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSKEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEIPAEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IVISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            4943..5476
                     /locus_tag="WGO65_001423"
     CDS             4943..5476
                     /locus_tag="WGO65_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ0327712.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRDKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5539..6723)
                     /locus_tag="WGO65_001424"
     CDS             complement(5539..6723)
                     /locus_tag="WGO65_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ0327713.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDTQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILGKQRAIVFGNFRMGNIRDVYDSSYDFSTVVNHISRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNGSYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6763..7167)
                     /locus_tag="WGO65_001425"
     CDS             complement(6763..7167)
                     /locus_tag="WGO65_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ0327714.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPKLRVITVHNDSGSPAFCRVIWHE
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1598 a         1763 c         2026 g         1827 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agacatattt
       61 ggattgcagg ctggtacgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccgata gacgcgccgc tgcaaaacat ttgcgcgttg gcattgccgg aagaaagcag
      181 gctgctgttg aaatcttccg tccattccca aatcagcccg tgcatatcat aaacgcccca
      241 gtagttcggg cggcctttgc cgacatcgtg caggcctttc cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggtgcaata
      421 ggcgttggcg gcaaaccagg aaacattggt taccggctgt tttaattcgc ctgccctggg
      481 tgcgtagctg cggctgccgt ttttcatcca atgcttcaga taagcgggtt ctgcctgttt
      541 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagccgccgc
      721 ttgagtgccg gcgagtgccg caccgaggaa aaataaccgg acgtacttca taaagcctcc
      781 tgacaggcgg ttagcattga tttttcagac ggcattttaa acgaaaagct gcgaatgccg
      841 tctgaagaat caatcttccg aaaggaaaga ttggttgtta aaaaaccact gccgtgcgta
      901 atgaagtacg gcggcagtgg tttgtcccgc taatgacggg tatccggttt aataaacgct
      961 tttttcggat gcagaggctg ccggggcaga agcagccggt gcggaagcag caggggcaga
     1021 agccgcgccg ctgccggcgt aagcggtatc actcaatttt tgagtcataa tttcagggtt
     1081 ttccgcaccc tctactttca attggcccaa cgcgcctttg ttgaatgcgc ggaagatgga
     1141 gtggtcgacc aaagtgtagc tgcccgggat gtcgactttg aattcgacaa tggcggagcc
     1201 tccggcagga acgatggtgc tttgtacgtt ttcgttaatc agcttgccgc cttcaacata
     1261 aactttgtcg aagatttcgc cgatgacgtg gaaggaagac accaggttcg gaccgccgtt
     1321 accgacgtac atacgcacgg tttcgcctgc tttggctttc agcgcattat cgccggcgat
     1381 agaacctaca tgaccgttga atacgacgta ttcaggctgt tcggcaacgg ctttgtccat
     1441 gtcgaacggt tgcaggcctt gcgcgccttt tttgcctttg gtgtagaagt cgccttgaac
     1501 gatgtagaac tctttatcca ctttcggcag gccttcttta ggctcgacca aaatcagacc
     1561 gtacataccg ttggcgatgt gcataccgac cggcgcgacg gcgcagtggt agatgtacag
     1621 gcccggttgc agggctttga agctgaatgt ggaagtacgg cccggggcgg taaaggtcgc
     1681 ggctgcaccg ccgccctgac cggttgcggc gtggaagtcg acgttgtgcg gaacggtaga
     1741 agacgggttg ttggagaatt cgacttctac agtatcgcct tcgcgtacgc ggatcatgcg
     1801 gcccggaacg tcgccgtcaa atgtccagta gtggtattcc accccgtcgt ccattttcat
     1861 ggttttttcg acggtttcca ttttcacgcg gactttggca ggatagtcgc ggtcgattgc
     1921 gggaggtact tcgggagcgt gggtggtaac cgcatcgatg acgggcagtt cgcctgcagg
     1981 cgtttcggcg gcagcttgtg cggcggaact tgcggcttct gcggaagcgg caggggtttc
     2041 ggcaggagct tgagcagctt gttcgccacc gcaggcggct aaagcaaaca tagaagcgat
     2101 aattgcagct aaggcttggc gtttcatgat gttttccttt tgtaagaaaa gtaaattgtc
     2161 ctgaaaggat tggttaagaa cttggtgtga ttatgcggtt gccccattgc gggcattttg
     2221 atttaagtca aataattcat aaaatatgaa cttaaaatga cacttctaat actttataac
     2281 tactaaaaat tacaatgaat aggctgattt gccagggctt cggcagagca ggcaaaggca
     2341 gggcaatttc cgagtaaaga ataatgatag ttattatcat atatgtattt gttttatatg
     2401 tgagtttgtt tgaacaaatg tttatttgcg cggtaaaccg tgctacaatc tttaacattc
     2461 atattctgtg aattttaaac aactgcttta ttaaaaggag ctctcaaaat gggacagtac
     2521 aagaagctgt ggtacttgct gtttgccgtt ctggcagtat gctttaccat tcttggctat
     2581 atgggcagcg aggtttataa gaaagccccg ccttaccccg aacaggtcgt ttccgcatca
     2641 ggcaaggtct tgatgacgaa agacgatatt ttggcaggtc agtctgcgtg gcagagtacc
     2701 ggcggtatgg aagtcggttc gattctgggc cacggcgcat atcaggctcc ggactggacg
     2761 gccgactggc tgcatcgtga gttgtccgct tggttggatt tgaccgcgca acagacttac
     2821 ggcaaaaaat tcgacgaggt ttcccccgaa gaacaagccg ttctgaaaac ccgcctggct
     2881 gacgaatacc gcaaccaaag ccgcgtgaag gaagacggtt ctgtggtcat cagtgatacg
     2941 cgcgtgaaag ccatcgaaag catcctgccg tactaccacg gtgtttacgg cgacgatccc
     3001 gcactgcaaa ccacccgcga acactttgca atgaaaaaca acacattgcc aagcaaagaa
     3061 gcgcgtgaaa aactgttcga cttcttcttc tggacttctt ggtctgcttc gaccaaccgt
     3121 cccggcgagg tctttaccta caccaacaac tggccgcatg agcctttaat caacaacgta
     3181 ccgactactg aaaactatat gtggtcgttt accagcgttg tattgctctt gatgggcatc
     3241 ggtttgttga tgtggggtta ttccttcctg accaaacacg aggaagtgga aatcccggct
     3301 gaagatccga tttccaaaat ccaactgacc ccttcgcaaa aagcattggg caaatacgtc
     3361 ttcctgacgg ttgccctgtt tgtggtacaa gtattgctgg gcggcctgac cgcgcactac
     3421 accgtcgaag gtcagggttt ctacggcatc gatgaggcgt tgggctttga aatgtccgac
     3481 tggtttccct acgccctgac ccgtacttgg cacatccaat ccgccatctt ctggattgca
     3541 accggctttt tgacggcagg tttgttcctc gcgccgattg tcaacggcgg taaagatccc
     3601 aagttccaac gtgccggcgt aaacttcctc tatatcgccc tcttcatcgt ggtcggcggt
     3661 tcttacgcgg gcaacttctt cgcgttgacg cacatccttc ccccggaatt taacttctgg
     3721 ttcggacacc aaggttacga atacctcgat ttgggacgtt tctggcaact ccttttgatg
     3781 gtcggactgc tgttatggct gttcctgatg ttgcgttgca cggtttccgc ctttaaagaa
     3841 aaaggcgtgg acaaaaacct gttggcaatc tttgtcgctt ctatggtcgg cgtgggcgtg
     3901 ttctacgcgc cgggcctgtt ctatggtgaa aaatccccga ttgccgttat ggaatactgg
     3961 cgttggtggg tggttcacct gtgggtggaa ggcttctttg aagtatttgc cactgccgcc
     4021 tttgcctttg tcttctacaa tatgggcttt gtccgccgca gtaccgccac tgcctccact
     4081 ttggccgctg ccgccatctt tatgttgggc ggcgttccgg gcacgctgca ccacctgtac
     4141 ttctccggct ctacctccgc ctctatggca atcggcgcct gcttctccgc tttggaagtc
     4201 gtgccgctgg tgttgctggg ccgtgaagca tacgagcatt ggtcttacca acacctgtcc
     4261 gactgggcaa aacgcctgcg ttggccgctg atgtgcttcg tggcagtcgc cttttggaat
     4321 atgatcggtg ccggcgtatt cggcttcctg atcaatccgc cgatttccct gttctatatt
     4381 caaggtttga acacttctgc cgttcacgcg cacgccgcct tgttcggtgt gtacggtttc
     4441 ttggcattgg gcttcgtatt gttggttgcc cgttatttga aaccgaacgc gcagtttgat
     4501 gacaaactga tgacttgggg cttctggctg ctcaacggcg gcttggtcgg tatgattgtt
     4561 atcagcctgc tgcctgtcgg cgcgattcag gcatacgcct ccatcacgca cggtctgtgg
     4621 tatgcccgca gcgaagagtt cctgcaaatg gaaatcctcg ataccttgcg ttgggtacgt
     4681 acggcagctg acttgatctt tatcggcggt gcaatctgcg ttgccatcca agccactaaa
     4741 atcgtattcg gccgcgacaa ataagttttg cggtatgaaa tgagcaactg cacccgtttt
     4801 cgggtgcagt tttttattgc ggattgggac gggaatggaa aaggatgtgg attttttatt
     4861 ttaatcggct atacaattgc tgctttcatg ccgtctgaag cacgtttcag atggcatcgg
     4921 tttacatcag ataaggataa aaatgggtat ttacgatttt caaatgaaag atgcagaagg
     4981 caatgcggtt gatttgtcag gctatcgcga caaggttctg ctgattgtca acacggcaac
     5041 gcgttgcggt ctgaccccgc aatacgaggc tttgcagaag ctgtatgcac aatataccgc
     5101 agaaggcttg gagattttgg attttccgtg caaccagttc cgcgaacagg ctcccgaaag
     5161 cagcggagaa attgcccaag tgtgtatgat gaagtttggc acgaagttta agattttcga
     5221 caaaatcgaa gtcaatggag caaataccgc gcccttgtat gcctacctga aatccgtcaa
     5281 accgcaagat aaaggcaatc atctgtttaa agatttcgtg ttgaagctcg cagctttggg
     5341 agagaagcgc gacgaaggcg acatcaaatg gaactttacc aaattcctcg tcaaccgcga
     5401 cggcgaagtg gtcgagcgtt ttgcgcctag tgttacgccc gaagaaatcg aagccgatat
     5461 tcgggcattg ctgtaatctg ccgccgtctt gggaaatgcc gtctgaaggc ttcagacggc
     5521 atttgtccgt ttgcgggttt attccgagat atcggcaaca ccgctttcgg ggaagatggg
     5581 caaatccggc ggtgggagca aggtatccaa agtcagggcg gacgcatcga gtgtggggta
     5641 gccttcaaac gcgaccgaat aactgccgtt tccgttcata cggattcggg cgtgcgcgcc
     5701 cagagggaaa gtaattttgt cggcaatatg tccgaacggg aaacctgtca gcacaggaat
     5761 ttttgccgta cgagaaatat ggttgaccac ggtagaaaaa tcataagacg aatcgtatac
     5821 atcgcggata ttgcccatac ggaaattgcc gaacacgatg gcgcgttgtt tgcccaaaat
     5881 acccgaaaga tacagcgtat tgagcatacg ttcgatgcgg tagggctgtt cgccgacatc
     5941 ttcgaggaac aaaatgccgc cgtcgatgtc gggcatataa ggcgtgccgg cgagcgaggc
     6001 gaggacgctt aagttgccgc cccacaatat gccttcggtt tcgacatcgg cgcgttggat
     6061 ataaggaaca tcaacggtca ggcggttttg ggttgcaccc ttgataaagg catccatcgt
     6121 aaacgcaccg ggggcgggtt tgccaaaatc gctataagcc atcgggccgg caaaactcat
     6181 catattgcct tttgccaaca atgccagctg gacggcgcat acgtcgctga atccgaaaaa
     6241 cagcgtgccg tgttcgcgca tccttgcgcc gagcgaagca aaatcgatat gcggcagaat
     6301 ccgcgccgca ccgtaaccgc cgcgcaagcc catcagcact ttaggcgtgg cgacgcggcc
     6361 ggaagcgacc tcttggaaat cggcggcacg ttgcgtgtcc gtgccggcaa accgttggaa
     6421 acggcggctg cccgcctgtt ggttggttac ggtaaaaccg acattgtaaa ggcgggttaa
     6481 ggctgtgttg acgcggttgg tgtcttcggc aaatcctgac gaagcgacaa cgcgcagaag
     6541 gttgccgtcc gaactttgac ggcgtggcgt ttggagaggc acggttcggg ctttcacaac
     6601 ggaatgggaa gagggaaggg gcggaacgga tgtggcggat gtgccgcaag cctgaagcag
     6661 ccccgcgccg gcagcggcgg tgcaggtttt cagaaaacgg cggcgggagg tcggttcggt
     6721 cataatttcc ctttcatacg gtttcagacg gcattggcgg gcttaaagca gctttttaag
     6781 ctgttccgtc caatcttgcg gcagggtcat accgtgtttc cggacggggt ttgcccaaat
     6841 cggcgcgggg aaagacgcat cgttttcaaa gcgggcgata atatgccaat gcaggtgcgg
     6901 cacgacattg cccaagctgg cgaggttgat ttttgccggc cggaacactt ggcgcatagc
     6961 ggcttcgact ttgtacacca tttccatcaa ttcgccgcgt tccgctgccg aaaggtcggt
     7021 catttcggcg atgtgttcat gccagataac acggcagaat gcaggcgaac cgctgtcgtt
     7081 atggacggtg atgacgcgca gcttgggggt ttgcagcaaa acgtcttcat tttgcgccgt
     7141 gcagatgggg cagggttggg cggtcatttt tccttattcc tgaaagtgtt taaaaaacgg
     7201 taggataatt tctg
//
LOCUS       DAWFAN010000099         7088 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWFAN010000099 DAWFAN010000000
VERSION     DAWFAN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.099"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(69..428)
                     /gene="rnpB"
                     /locus_tag="WGO65_001426"
     ncRNA           complement(69..428)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WGO65_001426"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(507..1610)
                     /gene="prfB"
                     /locus_tag="WGO65_001427"
     CDS             complement(507..1610)
                     /gene="prfB"
                     /locus_tag="WGO65_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ0327715.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1713..2606)
                     /locus_tag="WGO65_001428"
     CDS             complement(1713..2606)
                     /locus_tag="WGO65_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ0327716.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRRIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2948..3397
                     /locus_tag="WGO65_001429"
     CDS             2948..3397
                     /locus_tag="WGO65_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0327717.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3471..3779
                     /locus_tag="WGO65_001430"
     CDS             3471..3779
                     /locus_tag="WGO65_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327718.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3895..4737
                     /locus_tag="WGO65_001431"
     CDS             3895..4737
                     /locus_tag="WGO65_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ0327719.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4727..5473
                     /locus_tag="WGO65_001432"
     CDS             4727..5473
                     /locus_tag="WGO65_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ0327720.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5495..6082
                     /locus_tag="WGO65_001433"
     CDS             5495..6082
                     /locus_tag="WGO65_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ0327721.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6088..6267
                     /locus_tag="WGO65_001434"
     CDS             6088..6267
                     /locus_tag="WGO65_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ0327722.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6499..6939
                     /locus_tag="WGO65_001435"
     CDS             6499..6939
                     /locus_tag="WGO65_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327723.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1808 a         1889 c         1614 g         1777 t
ORIGIN      
        1 acacaaccta aataaaaatg ccgtctgaac catatttcag gtttcagacg gcatttgcgt
       61 gtcggatgca caccggacgg gcggtaagcc gggttctgtc tcggacagtc attcctctag
      121 gcataccgtt accggtatgc tcaagcaacc tacccgaacg ctcggcgggc agcgtcattg
      181 cgttctgttt ggtcttgctc cgaatggggt ttggcctgcc gcatattgtt accaaatgcg
      241 cggtgcgccc ttaccgcacc ttttcaccct tgcctgtgct gccaaagcag ccatcggcgg
      301 ttttgctttc tgttccactt tccgtcgcgt taccgcgccc ggccgttaac cggcattcta
      361 ccctgcggag cccggacttt cctccccgta tgccttacgc gatacgcggc gactgtctgc
      421 ccgtcccgtg tgcggcgcgg attataacac gaaacgcaaa aatgccgtct gaaacggtac
      481 aggtttcaga cggcatacag cctaaactac acgccctgtt tcaggctggc ttcgataaag
      541 ccgtccaaat cgccatccaa tacggctttg gtgttgccga cttcgtagcc tgtacgcaag
      601 tctttgatac gtgaggaatc caaaacatac gaacggattt ggctacccca acctacatcg
      661 gatttacctt cttccaacgc ctgtttctct tcattgcgtt tgcgcatttc caattcatac
      721 agtttggact tcaacatttc catcgcggcg gctttgttgg cgtgttgcga acggtcgttt
      781 tgacattgca ccacaatccc cgtcggctcg tgggtaatac gcacggcgga gtcggttttg
      841 ttgatgtgct gaccgcccgc gcccgatgcg cgataggtgt cgatacgcaa atcggcgggg
      901 ttgatttcga tttcaatgga atcatcgatt tcagggtaaa cgaacacgga ggcaaacgaa
      961 gtatggcgtt tgttgttcga gtcaaacggt gaatagcgca ccaagcggtg aacgccggtt
     1021 tcggtacgca gcaaaccata ggcgtattcg ccttccactc ggatagtcgc gcggttgatg
     1081 cccgcgattt cgccgtcgtc ttcctcaagg atttcgattt tgaagccttt gcgctcggcg
     1141 tagcggctgt acatacggaa cagcatcccc gcccagtctt ccgcttccgt accgcccgcg
     1201 cctgcggtga tgtcgataaa gcagttgttc ggatcggcag gctggttgaa catccgtttg
     1261 aactccaaat ccgccatctg tttttccagc cccgccacgt cttcctgcac ggcggcaaaa
     1321 ccttcttcgt cgttttcttc gacggtcatt tcaatcagca tgcggttgtc ttcgatgccc
     1381 gaagcgatgt tgtcgagcgt caacacgatg ccttcgagga ttttgcgctc tttgccgatt
     1441 tcttgggcgc gtttcgggtc gttccaaagt tcggggtctt cggaaagacc gataacttct
     1501 tccaatcggt ctttcttacc ctgataatcc atataaactc ggatgtcttc gctgcgcttt
     1561 tccaaatcat tcagggtatt gttgagctgg ttgattactt cggcttccat gattcttttg
     1621 ttctttcaaa attttagggg cgtattgtac gggattcggg tatttttttc tatggataaa
     1681 gccttctgga aacacgttca gacggcatag cgtcaataac ggtatgccgc cagtttgcgt
     1741 ttgatttcag gcaatgcggc acgtgctgcc tcctcaccca accggatggc gcgttttttc
     1801 tgatcgaatc cgccgactgc acccaaatcc aaaacctgcg gtttgataac cacatccgcc
     1861 tgccccaact cattttgcaa cgcggaaacg ctcattacgt tcagcgtctg atcgagataa
     1921 gagaagaagc cttggctgat gtttttgctc ggacgggcgg aaatatcgac ggcaatcacg
     1981 aaattcgccc cctgccgccg ggcggcactg acgggcacgg gctgcgacag accgccgtca
     2041 acatatgtat gcctgccgat gataacgggt tggaacacat tgggaatggc ggcggaagcg
     2101 cgcacagcct gcccggcatt cccttgattg aaagcgacgg ccttgccggt ttcaaaatca
     2161 gtagcaacag cggcaaattt gatgggaaac tgctgaatcc gcctgccgcc gacttttcgg
     2221 ttgatgtaat tttgcagctt ttcgcctttg ataaaaccac tggtggacaa ggttaaatcg
     2281 actaaatcgg ttttgcctaa aatttcggct tccaattcga ggcggtcggg cgacataccc
     2341 gatgcaaaaa ggctgccgac aatcgaacct gccgatgtac cggtaaccac cttcacagga
     2401 ataccgtttt ctttcaaaac cttaataata cctacatggg caaatccttt agatgcgccg
     2461 ccaccgagtg ccaaaccgac cactgcggcg ggtttggcgg tttgcaccgg cttgcgggcg
     2521 gcattatttc ccgccgtgcc gcaggcggca agcaacgcgg cggcggcgat tgccaaaagc
     2581 ggtctgattt ttgaaaacgt taccatattt tccattcctt tatatatcgc accccgtcaa
     2641 aaagagggat tgcttttctt aacaaccccc tttgacagcc aagcaaatgg gggctttgtt
     2701 aagtcatcat caaaattaat atttcttttt ttcctttacg gaaattatat ttgaaggcat
     2761 actatccaag gtgggaatta tctcacaaca ccgccgttat ccaaatatcc cgcctttttc
     2821 ccttcctttc catcaaaata ctttcttttt atattcatta acttgttaaa tcattggctg
     2881 ccgggtgtca gtttttccga caaaatccgt ctaatggggt atcaacagaa ccaaaacagg
     2941 aacacttatg aaaatcggaa caacttggca gacggcatcc gctatgctgg ttttgcgtct
     3001 gtttgccgca tatgaatttt tggaatcggg tttgcaaaaa tggaacgggg agaattggtt
     3061 ttctgaaatc aacgatcaat tcccattccc gttcaacttg ctgccggacg cgttaaactg
     3121 gaatctcgct atgtatgcgg agcttttact gcccgtactg ttgcttttgg gtttggcaac
     3181 gcgtctgtcg gcattagggc tgatggtcgt taccgccgtc gcttgggctg cggttcacgc
     3241 cggttcgggt tacaatgtct gcgacaacgg ttataaaatg gctttaattt atatcgtggt
     3301 attaatcccg ctgcttttcc agggtgcggg cggatggtcg ctggatacgc tgctgaaaaa
     3361 acggttttgc ccccaatgcc gtctgaagca agattgattc agtcgtggaa tctgacttta
     3421 aacattccaa ccttatctcg ttaacttgat attctgaaaa ggaaatagaa atgaacaaaa
     3481 acattgctgc cgcactcgcc ggtgctttat ccctgtcttt ggccgccggt gcagttgctg
     3541 ccaacaaacc ggcaagcaac gcaacaggcg ttcataaatc cgcccatggc tcttgcggcg
     3601 cgtccaaatc tgccgaaggt tcgtgcggcg cggctggttc taaagcaggc gaaggcaaat
     3661 gcggcgaggg caaatgcggt gcgaccgtaa aaaaaaccca caaacacacc aaagcatcta
     3721 aagccaaggc caaatctgcc gaaggcaaat gcggcgaagg caaatgcggt tctaaataat
     3781 cccacccctt caaaccaagc cgcgtttttc agtaaaatgc ggctttttta acggcaaaca
     3841 aagatttttt aacaagcaca tcattctttt gtgccatccg aaccgggtaa aaatatgatt
     3901 caacacgcag gcttgggcta ccgccgcgac ttggcggaag actttctctc gctttccgaa
     3961 aacagcccga tatgctttat cgaagccgca ccggaaaact ggctgaaaat gggcggctgg
     4021 gcgcgcaaac agtttgaccg tgtggcggaa cggctgccgc tggcgttgca cggattgtct
     4081 atgtcgctgg gcgggcaagc accgctggat actgatttga tagacggcat caaagaaatg
     4141 atgcgccgtt acgattgcac gtttttctcc gaccatttga gctactgcca cgacggcggt
     4201 catctttacg atttgttgcc gctgcccttt accgaggaaa tggtgcatca tacggcgcgg
     4261 cgtatccgcg aagtgcaaga ccgtttgggc tgccgcatcg ccgtggaaaa cacgtcctac
     4321 tatctgcatt ccccgcttgc cgagatgaac gaggtcgagt tcctcaacgc cgtcgcacgt
     4381 gaggccgatt gcggcattca tctggatgtg aacaatatct acgtcaacgc cgtcaatcac
     4441 ggcctgttgt cgccggaggc ttttttgaaa aacgttgacg cagatcgcgt gtgctatatc
     4501 catattgccg gacatgacgt ggaaacgccg gaattgttga ttgatacaca tggcgcggca
     4561 gttttgccga ctgtttggga cttgctcgaa cttgcctatg ccaagctgcc gacgattccg
     4621 cccaccctgt tggaacgcga ttttaatttc ccgccttttg ccgaactcga agccgaagtc
     4681 gccaaaatcg ccgattatca aacgcgtgcc ggaaaggaat accgccgtgc agcctgaaac
     4741 ctccgcccaa taccagcacc gtttcgccca agccatacgc gggggcgaag ccgcagacgg
     4801 tctgccgcaa gaccgactga acgtctatat ccgcctgata cgcaacaata tccacagctt
     4861 tatcgaccgc tgctataccg aaacgcggca atactttgac agcgaagaat ggagccgtct
     4921 gaaagaaggt ttcgtccgcg acgcgcacgc ccaaacgccc tattttcaag aaatccccgg
     4981 cgagttccta caatattgcc aaagcctgcc gctttcagac ggcattttgg cactgatgga
     5041 ttttgaatat acccaattgc tggcagaagt tgctcaaatt ccggatattc ccgacattca
     5101 ttattcaaat gacagcaaat acacaccttc ccctgcggcc tttatccggc aatatcgata
     5161 tgatgttacc gatgatttgc atgaagcgga aacagccttg ttaatatggc gaaacgccga
     5221 agatgatgtg atgtaccaaa cattggacgg cttcgatatg atgctgctag aaataatggg
     5281 ttcctccgcg ctttcgtttg acaccctcgc ccaaaccctt gtcgaattta tgcctgagga
     5341 cgataattgg aaaaatattt tgcttgggaa atggtcaggc tggactgaac aaaggattat
     5401 catcccctcc ttgtccgcca tatccgaaaa tatggaaggc aattccccgg gccaaaacca
     5461 tctatccgca taaaattacc ttgttcccga tactatgccg ctacccgacc tgaccgatgc
     5521 cgaattaata gagtcgcgta aactgcttct gcattttgcg cggcttcagt tgcccgacca
     5581 ccctgatttg gctgaagatt tagtgcagga aacattgctg tccgcataca gcgcaggcga
     5641 cagttttcaa ggcagggcac ttgtcaacag ctggcttttt gccatattga aaaacaaaat
     5701 tattgacgca ttacgtcaaa tcggaaggca gaggaaagtc tttaccacac tggatgacga
     5761 gctactggat gaagcatttg aaagccattt ttcccaaaac gggcattgga cgccggaagg
     5821 gcagccgcaa cattggaaca ctccggaaaa atcattaaac aacaacgaat tccaaaaaat
     5881 tctgcaaagc tgcctataca acctgcctga aaacaccgca cgggtattta ccctgaagga
     5941 aatactcggt ttttcatccg acgaaataca acaaatgtgc ggtatcagca cgtccaacta
     6001 ccacaccatt atgcaccgcg cccgagaatc attgcgccaa tgcctgcaaa tcaaatggtt
     6061 caaccaagaa aacccgaagt aaacgttatg aaaaaatgcc gcgatatcgc cctgcttctt
     6121 tccaaacatc aggaccggga aaccaccccg ggcgagaaga tttccatata cacacacctg
     6181 ctgttctgtc cgtattgccg tgaatataaa agacaacttc aaaccatcaa aagatcactg
     6241 gcaaaaacaa ccagaacttc aaaataaatg ccgtctgaaa aggcttcaga cggcataagc
     6301 tgacggaaaa aaatcaaacc gatttactgt tatcggcagt tcatccataa tacacgcttc
     6361 aaaagcagca tattcctcca tacggaatat ataaatacgc aaaacacgaa ggctgcatca
     6421 atttgcttta tttgccttat ttcacagacg gcgctacccc tcccgcccaa cccgttcttt
     6481 ctgaatgagc agatttcaat gattaaggaa accctaatgc gcccaatctt cctatctttc
     6541 gttttactcc ctattttgat aaccgcctgc agcacaccgg acaagtctgc ccgatgggaa
     6601 aatatcggca caatctcaaa cggcaatatt catacatata tcaataaaga cagcgtgaga
     6661 aaaaacggaa atctgatgat tttccaagat aaaaaagttg ttaccaatct aaaacaagaa
     6721 cgttttgcca acacccccgc atacaagact gccattgccg agtgggaaat ccactgcaac
     6781 aacaaaacat accgcttaag ttcgctacag ttgtttgata caaaaaacac ggaaatttcc
     6841 acacaaaact acacagcctc ttccctccgc ccgatgagca tcctgtccgg gacattaacc
     6901 gaaaaacaat atgaaaccgt atgcggaaaa aaactctgat tgcaacttat acacaaactt
     6961 acccacaaac cttatcataa aaatgccgtc tgaaatactg aaatatcagc atttcagacg
     7021 gcattttgcc attccctgaa aattatccac aaagttatcc acattatttt ttaaaaccgg
     7081 cttccatc
//
LOCUS       DAWFAN010000100         7054 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWFAN010000100 DAWFAN010000000
VERSION     DAWFAN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.100"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            140..721
                     /locus_tag="WGO65_001436"
     CDS             140..721
                     /locus_tag="WGO65_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0327724.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(783..1637)
                     /gene="folD"
                     /locus_tag="WGO65_001437"
     CDS             complement(783..1637)
                     /gene="folD"
                     /locus_tag="WGO65_001437"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ0327725.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1860..1937
                     /locus_tag="WGO65_001438"
     tRNA            1860..1937
                     /locus_tag="WGO65_001438"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1895..1897,aa:Pro,seq:tgg)
     gene            1962..2038
                     /locus_tag="WGO65_001439"
     tRNA            1962..2038
                     /locus_tag="WGO65_001439"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1996..1998,aa:Arg,seq:tct)
     gene            2066..2141
                     /locus_tag="WGO65_001440"
     tRNA            2066..2141
                     /locus_tag="WGO65_001440"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2099..2101,aa:His,seq:gtg)
     gene            2277..2783
                     /locus_tag="WGO65_001441"
     CDS             2277..2783
                     /locus_tag="WGO65_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ0327726.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2780..4558
                     /locus_tag="WGO65_001442"
     CDS             2780..4558
                     /locus_tag="WGO65_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ0327727.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4671..5495
                     /locus_tag="WGO65_001443"
     CDS             4671..5495
                     /locus_tag="WGO65_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ0327728.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(6257..7045)
                     /locus_tag="WGO65_001444"
     CDS             complement(6257..7045)
                     /locus_tag="WGO65_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ0327729.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT         1503 a         1826 c         2120 g         1605 t
ORIGIN      
        1 atgtatggcg taaaggaagc gttttaacgc cgtattggga aaatgtaaag ttctttatcg
       61 ggatttcttt aagtattggc gttttcggtg tcgtggcggt agaatcgggg tcagttaaga
      121 acaattatca ggaacggata tggaagaaaa aaacgactac accgacgcgg catccgacaa
      181 ccgcaacggg caggaaattg aagtcggaat tgccggcgca ggcgaccgga tacttgccgc
      241 gctgctgaac caattgttta cctttttggt tttgttggtg ccgtttgccg gactgattgc
      301 ttttgccatc aagaatgaag gcaggattgg cggcagtgag gaaatattcg ggctgctttt
      361 gggcatgact tccttttggg taggactggc gggcattttg gtttataccg tcgttcaaat
      421 ctactatatg agtcgggacg ggcagtcatt gggtaagaaa atcatgagaa tccgcgtgtt
      481 gaaaaccgac ggccgcaatc ccggttttgt cggcacggtt ttggtacgcg aaatcgcatg
      541 gtcggttttg gttgccatta ttgccgccgt tatcggtctt gcagtaggtg acaacggaga
      601 aaacgccatc aacctgctgg cattccttgc caactttgtc ctgctcttta tggtcaaacg
      661 cgaccgccgc acgctttacg acatactggc ggatacggtt gtcgtcaagc tgcccaaata
      721 aacggacacg gcaaaatgcc gtctgaaagc ttttcagacg gcatttttat cttcagaacc
      781 gctcaagcat cgtgcagtga agccgcgtgc agggtgtttt ccatcaatgt ggcaatcgtc
      841 atcggaccca cgccgccggg aacgggcgta atcatcgccg cccgttcttt tgccgtttca
      901 aattccacgt cgccgcacag gctgccgtcg tccaaacggt tgatgcccac atcgataacg
      961 accgcgccgg gtttgatcca ttcgcctttg acaaagttcg gaatgcccac gccgaccacc
     1021 aaaatatcgg ctccggcaac ctcgtctgcc agattttcgg ttgcgctgtg gcagaccgtt
     1081 accgttgcgc gcgccagcag caattccaac gcctgcgggc ggccgacgat attcgacgcg
     1141 ccgaccacga ccgctttttt ccccttcgga tcaatgccgt aagcttccaa aagcgtcatc
     1201 acgcccttgg gcgtacacgg gcgcatcagc ggcattttga ccgccagcct gccgacattg
     1261 taaggatgga agccgtccac gtccttatcc ggcgaaatac gttccaaaac cgcctggctg
     1321 tcgaggtgct tcggcagcgg tagctgaacc agaataccgt ccacttcgga atcggcattc
     1381 aggcggtcga ccagtgccag cagttcttcc tgcgatgttg attcgggcag ctcgtaagac
     1441 agtgatttga tgccgcattt ttggcaggca gttttcttgt tgcggacata aaccgcgctg
     1501 gcagggtcgc caccgaccaa aaccacggcc aggcaaggca tgtgcagatt gtcctgttgg
     1561 cgttgcgcta ccgcttcggc aaccgcctgc aggcgttttt gcgaaacttc tttaccattg
     1621 atcagttgtg ccgacatatc cgtccttttg ttaaacaggt agaaaatgct gaaaattatc
     1681 gcattttccg tttgttttta cagcaggatg ccgaaaataa atttgccctg aaataaaata
     1741 cggctgtttg gacggattaa atattttttc aaatcaaaaa actatatttt tcatcttggt
     1801 gtcttgaaaa taaatattga cgcatctttc cctgagttgt atagttcgct tcttcaagtc
     1861 ggggcgtagc gcagcccggt tagcgcatct gctttgggag cagagggtcg tgagttcgaa
     1921 tcccaccgcc ccgaccaatc aatcagcacg aatcggcatg agcgcccgta gctcaaccgg
     1981 atagagcacc gaccttctaa gtcgggggtt acaggttcga ttcctgtcgg gcgtgccaag
     2041 aatttgattg aaaaccgttt ttatggtggc tgtagctcag ttggtagagc cccggattgt
     2101 gattccggtt gtcgtgggtt cgagccccat cagccacccc agataaaggc ccgtactttt
     2161 cggtacgggt cttttctcgt ttgcgcgtac tgtccgcgcc gccggctttg cctttcgcat
     2221 atgcttctgt atcatgtggg tttttctgtc ttttatggtt tttgtcggag gctttgatgg
     2281 ttgacgcttg gtctgtccct gattttgaat cgaaaatctg tccgcccgag gcgttggcgg
     2341 cgcgtttggc gttgttgccg cgcccgctgg tgtttaccaa cggctgtttc gacatcctcc
     2401 acagggggca cgttacttat ctggcgcagg cgcgttcgat ggggggcgcg ttggtgctgg
     2461 cgttgaatac cgatgcttcg gtgcggcgtt tgggcaaggg cggcgaccgc ccgattaatc
     2521 ctttggagaa ccgtgccgcc gttgccgccg ctttggaaag tgtggatttg gtaacgtggt
     2581 ttgacgggga tacgccggcg gcgttgattg aggcggtcaa acctgagatt ttggtcaagg
     2641 gcggcgattg ggctgcggat aagattgtcg gtgcggcaga aacgttggcg cgcggcggtc
     2701 aggtgttttc aattccgttt ctgcaccaga cttcgacaac gaagactttg gcaaaaatcc
     2761 gtgcggcaga gggcggaaaa tgacggtttt gaagctttcg cactggcggg tgttggcgga
     2821 gcttgccgac ggtttgccgc aacacgtctc gcaactggcg cgtatggcgg atatgaagcc
     2881 gcagcagctc aacggttttt ggcagcagat gccggcgcac atacgcgggc tgttgcgcca
     2941 acacgacggc tattggcggc tggtgcgccc attggcggtt ttcgatgccg aaggtttgcg
     3001 cgagctgggg gaaaggtcgg gttttcagac ggcattgaag cacgagtgcg cgtccagcaa
     3061 cgacgagata ctggaattgg cgcggattgc gccggacaag gcgcacaaaa ccatatgtgt
     3121 gacccacctg caaagtaagg gcagggggcg gcaggggcgg aagtggtcgc accgtttggg
     3181 cgagtgtctg atgttcagtt ttggctgggt gtttgaccgg ccgcagtatg agttgggttc
     3241 gctgtcgcct gttgcggcag tggcgtgtcg gcgcgccttg tcgcgtttag gtttggatgt
     3301 gcagattaag tggcccaatg atttggttgt cggacgcgac aaattgggcg gcattctgat
     3361 tgaaacggtc aggacgggcg gcaaaacggt tgccgtggtc ggtatcggca tcaattttgt
     3421 cctgcccaag gaagtagaaa atgccgcttc cgtgcaatcg ctgtttcaga cggcatcgcg
     3481 gcggggcaat gccgatgccg ccgtgctgct ggaaacgctg ttggtggaac tggacgcggt
     3541 gttgttgcaa tatgcgcggg acggatttgc gccttttgtg gcggaatatc aggctgccaa
     3601 ccgcgaccac ggcaaggcgg tattgctgtt gcgcgacggc gaaaccgtgt tcgaaggcac
     3661 ggttaaaggc gtggacggac aaggcgttct gcacttggaa acggcagagg gcaaacagac
     3721 ggtcgtcagc ggcgaaatca gcctgcggcc cgacgacaga ccggtttccg tgccgaagcg
     3781 gcgggattcg gaacgttttc tgctgttgga cggcggcaac agccggctca agtgggcgtg
     3841 ggtggaaaac ggcacgttcg caaccgtcgg tagcgcgccg taccgcgatt tgtcgccttt
     3901 gggcgcggag tgggcggaaa aggcggatgg aaatgtccgc atcgtcggtt gcgctgtgtg
     3961 cggagaattc aaaaaggcac aagtgcagga acagctcgcc cgaaaaatcg agtggctgcc
     4021 gtcttcggca caggctttgg gcatacgcaa ccactaccgc caccccgaag aacacggttc
     4081 cgaccgctgg ttcaacgcct tgggcagccg ccgcttcagc cgcaacgcct gcgtcgtcgt
     4141 cagttgcggc acggcggtaa cggttgacgc gctcaccgat gacggacatt atctcggggg
     4201 aaccatcatg cctggtttcc acctgatgaa agaatcgctc gccgtccgaa ccgccaacct
     4261 caaccggcac gccggtaagc gttatccttt cccgaccaca acgggcaatg ccgtcgccag
     4321 cggcatgatg gatgcggttt gcggctcggt tatgatgatg cacgggcgtt tgaaagaaaa
     4381 aaccggggcg ggcaagcctg tcgatgtcat cattaccggc ggcggcgcgg caaaagttgc
     4441 cgaagccctg ccgcctgcat ttttggcgga aaataccgtg cgcgtggcgg acaacctcgt
     4501 cattcacggg ctgctgaacc tgattgccgc cgaaggcggg gaatcggaac atacttaaaa
     4561 aatacaccct ttttcaacac ggggcggcat ccgtccgaat ccgtccggat tctgccgccc
     4621 gacagactgc cgcccaggcg ggcggggttt caaacattga ggaaattcta atgaaatggc
     4681 tcttcaccct tttggcggta ctcaatattg ccgtattcgg cggcacggtg ggctataaat
     4741 tggccgttaa agcgacgggc ggcgtgccgg aaaaccgggc cgtcgaaaac acgccccccg
     4801 caacgcccgc cgccgacaat gcggcggcat ccgtggagga tacggcggca ctgctcaaac
     4861 cgggcgacat cctgaccgaa gagcaggccg agcagttgcg cttgaaaaag gaagcggaac
     4921 agaaaaaact gagggagaaa aaacagcgtg aagaaaaagc ccgccgcgaa aaactcgccg
     4981 ccgaaaaggc gcaggcggaa cgcgaaaacg gcgcggcgga tgccttatgc gccgcgcagg
     5041 caagcctcac gatggacgaa gacgactacc accgcatcaa agggcttttg ggcaaatggt
     5101 cgcacgttgc cagcaggagc gttgaaaaac gcaccgccaa agccaaacct gccgacaaaa
     5161 cctaccgcgt cgtcctgccc gtttccgccg atgccgaaaa tcaggcggcg gagctgtctg
     5221 ccaaaggttt caaccccata ccgtttgacg gcgcattgag tttgggtgtc ggcaacagtc
     5281 gggaaaacgc ccaagccctg caaaaccggc ttgccggcgc cggattcggc ggggcgcata
     5341 ttgtcgaaca ctttgccgaa gccgacaggc aggacgattc tttgagcgtg tcgcgtatga
     5401 cggttttgtt taccggcgtg aatgccgccg atgcggacga aatccgtaaa atcacgtccc
     5461 tatacggcaa actgaacctc aagtcttgca aataggcgcg aaagccggac gataaacgcc
     5521 aaacccaccg cgaaggtggg tttggcgttt cggttttgag tatctgatat ataaaaaggg
     5581 aatgtatgtc tcgtatatgc cgactccaag tgtgaaagtg atgatgggga aatatggtgt
     5641 ttaatggttc ggaacggacg gcttccgagt ggttttgatt atacgctaat aataataatt
     5701 atcaataaaa atttaaaaat aaatgcagat tttttgtaat acgatgaaaa ataaacaaat
     5761 taacctgtcc gccgatgttt gtgattcggg tcggcaaact ttcattgtgt cggacaaatc
     5821 ggggtcggca ggcatagctg agggcttcgt gcctgacgga aataagcctg ccgtgtttga
     5881 acatccgtcc gatttggcgc ggacagggtt gtcattcggg ggtgtttgag cgtggattcg
     5941 gggatgcaaa agcggtaaaa ccgttgcgga tgaaggctta cggacggctt taggtatttt
     6001 taagtgtatt caaacaacaa caaaacgctt cttctattgc cgtatttagg gcttgcgcgg
     6061 cagcctgcac accgtggagg ccgagtgttt cgttggttgc ggcatcggga cgggcggcaa
     6121 ggttttccgg ccggtttttc cattttttcc gacagagccg tgatgcttgg gaatggcggt
     6181 ttgcccgttt cagaccgccg ccgttttggg ctgcgatgcc gtctgaaccg ttcagacggc
     6241 atttttgccg cctttttcaa tattccgccg aatgccaaaa cggctgtccg actgtcggcg
     6301 tgctggcttg cgctttgtcg cgtgcttcga ccggtccggc ttcaaatgcc agccgtccgg
     6361 attcgacggc gagtgcgaag gcgcgtgcca tattgaccgg atcgccgctg cgggaaacgg
     6421 cagtattcaa aagcacgccg tcaaaacccc attccatcac ttgtgccgcc tgtgagggca
     6481 aacccaagcc cgcgtcgata atcagcggcg tgtcgggcag gcgttcgcgc aggacgttca
     6541 acgcgtaggc gtgaaccgcg cccaaacccg tgccgatcgg agccgcccac ggcattaacg
     6601 cctgacagcc cacatcgagc aggcggcggc aggcaatcag gtcttcggtg caataaggca
     6661 gcactttgaa gccgtcttta atcaggattt ccgccgcttc gacaagttgg aacacatccg
     6721 gctgcaaggt gtcgtcgtcg ccgatgagtt cgagtttaat ccaatcggtt tcaaacactt
     6781 cgcgcgccat ttgcgccgtc gttaccgctt cctgcacgct ttggcagcct gccgtgttcg
     6841 gcaggacggg aacgccggtt tcttgaagca gcgaccaaaa cccctgaccg tgcgcctcgc
     6901 cgccgcttcc cgtgcggcgc agcgagacgg taatcatcgc gggttgggcg atacggacgg
     6961 attgtttgag gatttcaggg gtcgggtagg cagccgtgcc gagcagcagc cgtgaaggga
     7021 aagtttctcc gtatagggtg agcataatgg gttc
//
LOCUS       DAWFAN010000101         7001 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWFAN010000101 DAWFAN010000000
VERSION     DAWFAN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.101"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(76..1497)
                     /locus_tag="WGO65_001445"
     CDS             complement(76..1497)
                     /locus_tag="WGO65_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ0327730.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1857..2216)
                     /locus_tag="WGO65_001446"
     CDS             complement(1857..2216)
                     /locus_tag="WGO65_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ0327731.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFYRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2213..2818)
                     /locus_tag="WGO65_001447"
     CDS             complement(2213..2818)
                     /locus_tag="WGO65_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ0327732.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2865..3347)
                     /locus_tag="WGO65_001448"
     CDS             complement(2865..3347)
                     /locus_tag="WGO65_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0327733.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3551..3648
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3859..4959
                     /gene="gcvT"
                     /locus_tag="WGO65_001449"
     CDS             3859..4959
                     /gene="gcvT"
                     /locus_tag="WGO65_001449"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ0327734.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            5121..5507
                     /gene="gcvH"
                     /locus_tag="WGO65_001450"
     CDS             5121..5507
                     /gene="gcvH"
                     /locus_tag="WGO65_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ0327735.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANVADYDGLLTAEQYAGEVD"
     gene            5652..6899
                     /locus_tag="WGO65_001451"
     CDS             5652..6899
                     /locus_tag="WGO65_001451"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ0327736.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFSAAVLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     AAASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1787 a         1674 c         1968 g         1572 t
ORIGIN      
        1 tgtcggaaag gaaaaatatt gatttgatta tgccggaatt taaaatgccg tctgaatgtt
       61 cagacggcat agcgtttaca gcagtttgaa aacgaaaaag ataagggtat gtacgatgaa
      121 gacgggtgtc aggaaggcga ccgaccacat catatagccg aagaaagtcg gcatcggtac
      181 gccgcgctgt tcggcaatgg ccttgaccat gaagttcggt gcgttgccga tgtaggtcag
      241 tgcgcccatg aataccgaac ccatagaaac cgccagcagc gaatgaaaca gggtacccgt
      301 catcaaggct tgggcatcgc cgcccgccat attgaaaaaa acgagataag tgggcgcgtt
      361 atccaagaat gccgacaata tgccgctcat ccaaaaatac atcacattaa tcggatgacc
      421 tgccgtatcg tgaaccagcg ataccacccc gcccagcgcg cctgcctcgc ctgctttcag
      481 aatgctcagg acgggaaaga tggtgatgaa gatgccgagg aagagtttgc ccacttcggc
      541 gatgggttca aagttgaatt cgttgcctgc gcggacttgt ttgggcgtga ttgccataga
      601 tacggcggtc aatgcaatca ggatgacatc gcggacgagg ttttgcaggg cgtaacggct
      661 gccgaggatt tcaaatcccg ggtgttcggg tttccaaagg ccggacatta gaaccgcgcc
      721 gaccacgccc gaaagcagga ggaagttcca tttgccgaag atggcgattt tttcgggttt
      781 ttcctgttgt gccggcgtat cttgtgcaat gctttcctgt ttgaagaaac ggttgtcgat
      841 gaaatagaag gcggtcaaca ggacagcggt gctgatcagg acgggggcga acatatgttt
      901 gaccgtccac atgaaatcta cgcctttgag gaagccgagg aagagtgggg ggtcgcccaa
      961 aggggtcaga ccgccgccga tgtttgcaac caggaaaatg aagaagatga cgatgtgcac
     1021 gcggcgggta cggttttggt tggctttcag cagcggacga atcatcagca ttgctgcgcc
     1081 ggtcgttccc atgatagagg caagtgccgt accgacggca agcagggcgg tgttgagctt
     1141 gggtgtgccg ttcaagtcgc cccaaaccaa aatgccgcct gaaatggtgt acagggcaag
     1201 cagcagcagg atgaaaggga tgtattcttc aacgagtgcg tgtgcgacgg tatggatacc
     1261 ggcggacgcg ccaaaaacca aactgaacgg gatgaggaag agcaatgtcc aaaaggcggt
     1321 aattttgccg taatggtgat gccaggtatg cgaaaaaaac aagggaccca atgcgataga
     1381 cagcaaaatc agggcaaagg gcaggcccca cagcaggttt aggtttgcgc cgtccaaatc
     1441 tgcggcgtaa accgatgctg ggaaaagcat tagtgaaaac aggggtaggt ggcgcatcgt
     1501 gtttcctcga ttcaagcact gccttgcgcg gcgcgtggga gtgatacagg caccgtgccg
     1561 cccggacata ggcggactgt gctgcctgtt tgtttttgaa aaaagttttc cgattgattg
     1621 taaagtaaat aatcaggata aaccagtttc ccaaaccgga aggcggcggg aaggcggatt
     1681 gctgtgcttg ggaatatatg ttctttttga taaataattt tatttaaaac aaataaatat
     1741 atgtctttaa taaataatct atcgaaaacg aaaaatgaat ttattttaac atatatttgc
     1801 aatgaaacag gtttgccccc cccgtttgtt tgcccttatc cctttcagta cggcattcaa
     1861 gattcgggcc tgcgccacat ccatatggcg acaagggaac aaaaaaccga tgaaaccgcc
     1921 ccgacccacc agcgttgggg aaactgccaa aacattatca ggcaggatgc ggtcatcata
     1981 ctgatggcga atattttggc tttgcgcggc actgcgccgt tttgttccca gttatgaacc
     2041 atcgggccga aatagcggtg ccggtgcagc cagcggtaaa agcgcgggga tgcctttgcc
     2101 cagcaggcgg cggagagcag tacgaacggc gtggtcggca acagcggcaa aaaaatgccg
     2161 atgataccca acagcaggga gatgcagccg caggcaatta aaagataacg tatcattttg
     2221 aaatattttt cttattgtgc ggataagggc aggatgtgat accgagtttt gcccagcctt
     2281 catgtcccat tttttccagc agggcgatat tgcgttcgaa tattgccgaa gcgtcgggaa
     2341 aggcttgtgc ggctttggca atgctgtctt cgcggatgag gtgcagcgtc ggatagggag
     2401 aacggttggt gtagttgccg atgtcgtcgg gttcggtatt ctgaaagcgg aattggggat
     2461 ggaacggtgc gatttggacg atgccttcca agccgttttc gataacggcg gcatcggcaa
     2521 tatcgagcat atcgttgaat acgtcgaaat cggggaatag ggtcgggtga accagcaggg
     2581 tggtttccag ttcggtggcg ggtgtattgc ccagtcgctg cagttcttcg tccaagtctt
     2641 ccaaaaaact gtcaaggtgt ttggcttcgc tgattgcgat gcggacaagg tttttaacgt
     2701 ggggggcttt ggcaaaggga cacaggttca gaccgatgac ggctttttcc aaccattgtc
     2761 cggtgtgttc ggcaacagca tctttatttt cggaagtatt gatattcatt attgtcatgt
     2821 aaatgtgttt gcagattgca cgtgcgggaa aatcgggaag ggcactattc cttcagcagg
     2881 tggttgagcg gcagggaggt ggtgtgtttg atttctttta aaacaaagct cgattgcgca
     2941 tcttgtacgc cgtggtggga caggagcgta tccaaaacaa aatgggaaaa cgcgttcata
     3001 tcggtaaaaa acgcctgaag caggtagtcg gtttcccctg tcagggcgaa gcagctcaag
     3061 acttcaggcc attttcgaac cgatgcggca aagtcttccc gcgcgtcttt tgctttgcgg
     3121 atggaaacgc ggataaatgc ctgaagcccc aggttgacgg attccggaga cagcagcgcg
     3181 gcatattggc ggacgatgcc ggcatcttcc aactgcttca gacggcgcag gcacggagaa
     3241 ggcgaaagtg cgacacgttc ggacagttcg acattggtca gcctgccgtt ttcctggaga
     3301 acctgtaaga ttttaatatc ggttttgtct aaagtgagtt ggggcatatt tgcgttccgt
     3361 tttaaggaat tcggattgtc tgtccgtatg tttgcggcaa tccgcacaga tggagaccat
     3421 attaacatat aaaaagttat accgtcatcc gggacaaatt ttgttttcgg aaaatcatgt
     3481 gaaaacagag gcggtcggtt tgcatctctt ttaagacggc ttgcccaaac cgccgattca
     3541 agacataatc gggaaatgtg caggagagtg ttacacccaa ctacaatgta accaccgaag
     3601 gcgcagacac ccttaaatcg ctcaggtatc agggactgca cattgaaaca aacaatctgg
     3661 agagcggcgt tggaataacg tccaccgaag gggagaaggc cgtctgaacc accattcaga
     3721 caaccgcgca aagcagtgag cagactggtt tgccatcatg cggatacggc cgaaaatctc
     3781 aggttcaagg acagataggg tcatccgcgc acaggtgcgc gggtggcatc tgaacaaaaa
     3841 atccggagaa acttgagaat gattactctg aaaaccaccc catttcatca agcccatcaa
     3901 gatgcaggcg cgaagccggt agattttgcc ggctgggagc tgcccatcca ttatggttcg
     3961 caaatcgccg aacacgaagc cgtgcgcacc gacgccggta tgtttgacgt atcccatatg
     4021 cttgttaccg acgtagcagg cgcaaatgcc aaagcctttt tccgcaaatt gattgccaac
     4081 gatgtcgcca agctcgcttt tgtcggcaaa gccctttatt ccgctttgct caacgacaac
     4141 ggcggcgtga ttgacgactt aatcgtttac cgcaccaacg aagccgaaac ccaataccgc
     4201 atcgtgtcca acggagcgac tcgcgaaaaa gacacggcgc aattccacaa agtcggacaa
     4261 gagttcggcg ttgcctttaa tccgcgctac gacctcggca tgctcgccgt acaaggccct
     4321 aaagccatcg aaaaactcct gaccgtcaaa cctgaatggg cggatgtgat tcacggcctc
     4381 aaaccgttcc aaggtgcaga cttgggcaac gattggtttg tcgcccgcac cggctatacc
     4441 ggcgaagacg gcgtcgaagt gattctgccg ggcactgaag ccgtcgcatt cttcaaagcc
     4501 ctgcaagcag caggcgtaca accttgcggt ctcggcgcgc gcgacaccct gcgcatggaa
     4561 gccggcatga acctctacgg caacgatatg gacgacgata ccagcccgct cgaagcaggc
     4621 atgggttgga ccgttgattt gaaagacgaa agccgcgatt ttgtcggcaa agctgccttg
     4681 ttggcattga aagaaaaagg cgttgccgtc aaacaggtcg gtttgctgct cggcaaaggc
     4741 ggcatcctgc gcgcgcatat ggaagtgttg accgacaaag gcaaaggcga aaccaccagc
     4801 ggcgtattct cgcccagcct gaaacaatcc atcgccatcg cccgcgtacc gaaagacttt
     4861 gacggcgata ccgctaaagt gctgatgcgt ggcaaggaag tggacgtgcg cgtattgaag
     4921 ttgccgttcg tccgcaacgg tcaaaaacag tttgattaat atcggttcag acggcatttt
     4981 catttcatat gccgtctgaa agcaggtttt aattgttgtc cgatacggac gtttgtagaa
     5041 agccttgaac aaggtatctg tggatattga ttcatataga tgccgtctga aaataacccc
     5101 taccaatgga gaatcaaacc atgagcaaca acatcccagc agaactgaaa tatgttgcca
     5161 gccatgaatg gctgcgcctt gaagaagacg gtaccatcac cgtcggcatt acccaccacg
     5221 cgcaagagct gttgggcgac atcgtgttcg tcgagctgcc cgaagtcggc gcaaacctgg
     5281 ctgccgaaga gcaagccggt gtggttgagt ctgtaaaagc cgcatctgac gtgtacgcgc
     5341 cgattgcggg cgaagtcgtt gccgtcaacg atgacttgcc cggcgcacct gaaactgcca
     5401 acagcgaccc ttacggcgca ggctggttct tcaaaatcaa accggcaaac gttgccgact
     5461 acgacggtct gctgactgcc gaacaatacg cgggcgaagt ggactaaacc ctatcctgat
     5521 aatgggatgc ctgatttgag aaactgcact ttcagacggc aaggatgcgt ttggaagtgc
     5581 ggtttttgtc tgtgtcttca aggtcagtta ggctataata acggtctata tccatcttta
     5641 ccgatttttt catgcaactt accgctgtcg gactcaatca tcaaaccgca cctttaagca
     5701 tacgggaaaa gctggcgttt tccgccgccg tcctgcccga agccgtccgc aatcttgccc
     5761 ggagcaatgc ggcaacggag gcggtaatcc tttctacctg caaccgcacc gagctttact
     5821 gtgtcggcga ttcggaagaa atcatccgat ggcttgccga ttaccacagt ttgccgattg
     5881 aagaaatccg tccgtatctg tacacgctgg atatgcagga aaccgtgcgc cacgccttcc
     5941 gcgtcgcctg cgggctggat tcgatggtgt tgggcgagcc gcagatttta ggacagatta
     6001 aggatgccgt cagggttgct caagagcagg aaagtatggg taagaaactc aatgccctgt
     6061 tccaaaaaac cttttccgtt gctaaagagg tccgtaccga tactgccgtc ggcgagaact
     6121 cggtttctat ggcggccgct tccgtcaagt tggcggagca gatttttccc gacatcggcg
     6181 atttgaatgt cttgtttatc ggcgcaggcg aaatgattga gctggttgcc acttattttg
     6241 ccgccaaaaa tccccggctg atgacggttg ccaaccggac gctggcgcgt gcacaggagt
     6301 tgtgcgacaa gctcggtgtc aacgccgaac cgtgcctgct gtccgatctg cctgccattt
     6361 tgcatgagta cgacgtggtg gtttcttcaa cggcaagcca gttgcccatt gtcggcaaag
     6421 gtatggtgga gcgcgcattg aaacaaaggc agagtatgcc gttgtttatg cttgacttgg
     6481 ccgtgccgcg agacattgag gcggaagtcg gagatttgaa cgatgcctat ctttatacgg
     6541 tggacgatat ggtcaatatc gtccaaagcg gcaaggaggc aaggcagaag gccgccgccg
     6601 ccgccgaaac gctggtgtcc gagaaggttg ccgaatttgt caggcagcag cagggcaggc
     6661 agagtgtccc gttaatcagg gcattgaggg atgagggaga gaaagcgcgc aaacaggtct
     6721 tggaaaatgc gatgaaacag cttgctaaag gcgcaacggc agaagaggtt ttggaacggc
     6781 tgtccgtcca actgaccaac aagctgctgc attcgccgac ccaaactttg aataaggcgg
     6841 gggaagaaga taaggatttg gttcatgccg tcgcgcagat ttatcatttg gacaaataac
     6901 ggtgcgccgg gaaaaatgcc gtctgaagag gtttcagacg gcattttttt gtgccgcctg
     6961 acaacatcgt aaaatcccac attatatcga tgtaatcaca a
//
LOCUS       DAWFAN010000102         6751 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWFAN010000102 DAWFAN010000000
VERSION     DAWFAN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.102"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(111..470)
                     /gene="rplT"
                     /locus_tag="WGO65_001452"
     CDS             complement(111..470)
                     /gene="rplT"
                     /locus_tag="WGO65_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ0327737.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(483..680)
                     /gene="rpmI"
                     /locus_tag="WGO65_001453"
     CDS             complement(483..680)
                     /gene="rpmI"
                     /locus_tag="WGO65_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ0327738.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(827..1348)
                     /gene="infC"
                     /locus_tag="WGO65_001454"
     CDS             complement(827..1348)
                     /gene="infC"
                     /locus_tag="WGO65_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ0327739.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1366..3279)
                     /gene="thrS"
                     /locus_tag="WGO65_001455"
     CDS             complement(1366..3279)
                     /gene="thrS"
                     /locus_tag="WGO65_001455"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ0327740.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPITEDSSVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVAVKLSLRPEQRAGSDEVWDKAEQGLRDALTACGVE
                     WEELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3332..3408)
                     /locus_tag="WGO65_001456"
     tRNA            complement(3332..3408)
                     /locus_tag="WGO65_001456"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3372..3374),aa:Val,seq:gac)
     gene            3587..4702
                     /gene="tgt"
                     /locus_tag="WGO65_001457"
     CDS             3587..4702
                     /gene="tgt"
                     /locus_tag="WGO65_001457"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ0327741.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVIGQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQAQFHENRARGTD"
     gene            4712..5794
                     /locus_tag="WGO65_001458"
     CDS             4712..5794
                     /locus_tag="WGO65_001458"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ0327742.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            5986..6387
                     /locus_tag="WGO65_001459"
     CDS             5986..6387
                     /locus_tag="WGO65_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0327743.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(6450..6740)
                     /locus_tag="WGO65_001460"
     CDS             complement(6450..6740)
                     /locus_tag="WGO65_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327744.1"
                     /translation="MAKYFTETGIGVREHFDFFGEFVVSPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGGGNIGSMGLKVPR"
BASE COUNT         1711 a         1819 c         1582 g         1639 t
ORIGIN      
        1 taacgcaaat ttatttaaaa aaatagactt taaagaaaga aaatcaatca catagaattt
       61 ctgcaaacaa agaaaaaagg aagccgcagc ttccttttca attttttgga ttaagcagcc
      121 aaagcagctt tggctttttc aaccaattgt gcaaaagcgg ctttatcgaa cacggccaaa
      181 tcagccaata ctttgcggtc gatttcgata gaggcgcgtt tcagaccatt cataaatttg
      241 ctgtaagaca acccgttttc acgcgcacct gcattgatac ggacaatcca caattgacgg
      301 aattggcgtt tgcgttggcg gcggtcgcgg tatgcgtatt ggccggcttt cattaccgcc
      361 tgcttggcaa cgcggtaaac gtttttacga cggccgcggt agcctttggc taacgcgaag
      421 attttttggt gacgggcacg ggcggtaaca ccgcgtttta cgcgtggcat attctaaact
      481 ccttaagcgt agggtaacat tttagcaaca gaagccaaat cgcgatcgtt taccatagag
      541 gtaccgcgca gttggcgttt gtttttggtg gtctttttag tcaagatgtg gcgtttgaac
      601 gcatgagcgc gtttcacacc gccgttaccc agtactttaa agcgtttttt cgcgctagac
      661 ttggttttca ttttaggcat gggaaaactc cattcgttat cggataaggc attagggggt
      721 tttaaaacat cagtttcaaa cacttgaacc cacaatacca cggttttttg ccgcaattga
      781 aaacttactc cgaagcggca atcggagtaa gcggagaatt atagctttat tttttcttcg
      841 gcgcaatcat catcaccatt tggcgacctt ccattttggg aaaggactcg atttgcgcca
      901 cttcagccaa atcttctttt acacgttcca aaagttgcgc gccgagttgc tggtgagcca
      961 tttcacggcc gcggaaacgc aatgtcactt tgactttatc gccgtcggca aggaagcggt
     1021 taatgttgcg catcttgatt tgataatcgc cctcatccgt acccggacgg aatttaattt
     1081 ccttgatttg tacctgcttt tgatttttct tggcttcgtc gcgcttcttg gcttgttggt
     1141 atttgtattt accgtaatcc atcagtttgc atacaggcgg tttagcagtt ggggaaatct
     1201 ctaccaaatc gacatcctgc ccttcggcca tagccaaagc ttcacgaact gaaacgacac
     1261 caagctgttc gcctgactca ctgattaaac gcacttcttt ggcggtaatt tcgccgttga
     1321 ttcgtgcttc gcgttcttga gcgatgatga atactcctat aaaaattaat gattgacgag
     1381 ggcatcagtg atttcttgct gcaattgcgc aatgaaatca tccaaatcca aagaacccaa
     1441 atcttctgct ttgcggcgta ccgccacttt gttttcctgc ttttctttat cgccgacaac
     1501 gatttgataa ggaaaacggt attggctgtt gtcgcggatt ttgtaaccga ttttttcgtt
     1561 acgcaaatcc aactcggcgc ggaatcctgc cgcctgcaat ttggcagcca cttcacggca
     1621 gtaatccgct tgattttcgg tgatattcat aatcaccatt tgaaccggcg ccagccataa
     1681 cgggaaagag cctgcatggt tctcaatcag aatgccgata aaccgctcca aagaacccaa
     1741 aatggcgcgg tgcaacatta cagggcgagc gcggtcattg ttttcggtta catattctgc
     1801 atccaagcgt tccggtaaga cgaaatctag ttgcaatgta ccgcattgcc aagaacgacc
     1861 caaggcatct ttgacgtgat attcgatttt aggcccgtaa aacgcacctt cgccaggcaa
     1921 ctcttcccac tctacgccac aagcggtcaa tgcatcacgc aagccttgtt cggccttatc
     1981 ccaaacttcg tcagaacctg cacgttgttc agggcggagg gacaacttca cagcaacatc
     2041 atggaaaccg aactgtttgt agatgcgaac caacaattca ttgaatgcgc gcgcttcgct
     2101 gacgatttgg tcttcggtac agaaaatatg cgcatcatct tgcacaaaac cgcgaacacg
     2161 catcagacca tgcaatgcac cgcttggttc attgcggtgg caagaaccga actcggccaa
     2221 acgcatcggc aaatcgcgat atgagcgcaa accgttatta aaaatttgca catgacccgg
     2281 acagttcatc ggtttcaccg cgtattcgcg tttttctgaa ctggtcacga acatattgtc
     2341 tttgtagttg tcccaatggc cggatttttc ccaaaaggtt ttatccataa tttgaggcgt
     2401 tttgacctct ttataaccgg cggcgttcag ctctttacgc atatgctgtt caatcacttg
     2461 ccacaaagcc cagcctttag gatgccaaaa caccatgccc ggcgcttcgt cttgcagatg
     2521 gaacagatcc aattgcttgc caagtttgcg gtggtcgcgc ttttcggctt cttcgatacg
     2581 ttgaatatag gctttcaact cgtcttttgt cgcccaagcc gtaccgtata tgcgttgcag
     2641 catttcattg ttgctgtcgc cgcgccagta tgcgcccgcc aacttggtca gtttgaagtt
     2701 tttcaggaaa cgggtattcg gaacgtgcgg tccgcggcac atatcgacat attcctgatg
     2761 atgatacatc cccatcgctt ccacttcggg catatcgtca atcaggcgca gtttgtattc
     2821 ttcgccgcgc tcttgaaaaa ttttaatcgc ctccgcacgc ggagtcatga ttttgaccac
     2881 atcatagtct tgggcaatca attctttcat acgcgcttca atggcggcaa catcttccgg
     2941 tgtaaacggt ttttccgtgg cgatgtcgta ataaaagccc tcttcaatga cggggccgat
     3001 aaccattttt gcattaggat agagttgctt gacggcatgc ccgacaagat gcgcgcagga
     3061 atggcggatg atttcgatgc cttcctgatc tttcggagta atgatttgga cgcttgaatc
     3121 ttcggtaatc ggatcgcacg catcgaccaa tttgccgttt accttgcctg ccactgccgc
     3181 cttcgccaaa ccggcaccga tagacgcagc aatttgagcc acggtaacgg gggattcgta
     3241 ttggcggact gagccgtccg gcaaggtaat attcaacatc aaacgctccg aaagattaaa
     3301 aaataacggc ataaatgccg ttatggggat ttggtaggca cgattggatt cgaaccaacg
     3361 acccccacca tgtcaaggtg gtgctctaac caactgagct acgtgccttc aaagaatttt
     3421 tgcattttat cgggtcgttt taatttttgc aagaggtatg tgcatttctt tatgcaacat
     3481 tgtggcaaga tttggggatg tcgtctgaaa aaccgtggga attcatttat aatgccgtct
     3541 tttaaaacat atttcgcccc ctgtcgccca actgacattc aatcttatgc ttaaatttac
     3601 tttacacaaa aaagacggat acgcccgacg cggcacgctg gagttgaacc acggcaaaat
     3661 tgaaacgccg gtatttatgc ctgtcggtac ttacggctcg gttaaggcga tgaacccgca
     3721 aaacctgcac gacatcaagg cgcaaattat tttgggcaac acttatcatt tgtggctgcg
     3781 tccgggtttg gaagttatcg ggcaattcgg cggcctgcac ggttttatcg gctgggacaa
     3841 accgattttg accgactcgg gcggttttca ggttttttct ttgtcggata tgcgcaagct
     3901 gacggaagaa ggctgtacgt tcaaaagccc gattaacggc gacaagctgt ttttatcgcc
     3961 tgaaatttct atgaaaatcc aaacggtttt gaactcggac atcgcgatgc agttggacga
     4021 atgcacgccg ggcgaggcga cgcgcgaaca ggcgcgaaaa tcgctgcaaa tgagcctgcg
     4081 ctgggcggaa cggagcaaaa aagcctttga agatttgaaa aacccgaacg cactgttcgg
     4141 catcgtgcaa ggcgcgatgt atgaggattt gcgcgaagaa tcgctgcgcg gtttggaaca
     4201 gtttgatttt cccggccttg ccgtcggcgg tttgtccgtc ggcgaaccca agcccgaaat
     4261 gtaccgtatg ctgcgcgccg tcggtccgat tctgccggaa cacaagccgc attacctgat
     4321 gggcgtaggc acgccggaag acctcgtgta cggcgtggca cacggcatcg atatgttcga
     4381 ctgcgtgatg cccacccgca atgcacgcaa cggctggctg ttcacccgtt tcggcgattt
     4441 gaaaatcaaa aacgccaaac acaagctcga caagcgtccg atagacgaaa gctgcacctg
     4501 ctacgcctgc caaaacttca gccgcgccta cctgcaccat ctgcaccgcg ccggcgaaat
     4561 cttgggcgca cagctcaaca ccatccacaa cctgcatttc taccaagtca ttatggcgga
     4621 aatgcgcgat gccatagaac aaggcaaatt tgccgactgg caggcacaat tccacgaaaa
     4681 ccgcgcacgc ggcacagact gattcccggc aatgccgtct gaagccgctt cagacggcat
     4741 tttgccgctt taccctaccc gcactatttt tcagaaaccg atcatgcttc cattccttcc
     4801 agaaccctcc ctttcctata cgcaacaaaa ccgtaccgcc gttttgctgt tgaacctcgg
     4861 cacgcccgac gcgccgaccg cgcaggcggt acgaccttat ctgaagtcct ttctgaccga
     4921 ccggcgcgtc gtcgaactgc ccaaatggct gtggtatccc attctgcatg gtctggtact
     4981 gacgttgcgc cccaaaaaaa gcgcgcacgc ctatgaaaaa atctggttta aggaaggttc
     5041 gcccctagaa gtttacacag cacgccaagc tgccgcgctt gccgaacgta tgcccgacct
     5101 catcgtccgc cacgccatga cctacggcaa tccttcggtg gcagacgtat tgtcggaact
     5161 gaaggcgcaa ggcgcgggca gactgctggt catcccgatg tacccgcaat atgccgcttc
     5221 cagcagcggc gcggcagtag ataaggtgtg cgaacaatta ctgctgcaac ggaaccaaat
     5281 gagcgtccgc accgtttcgc gcttttatga cgataccggc tacattgatg cgatgaaaaa
     5341 ccacatcctc cgatattggg cggaacacgg acgtgggaaa aaactgatgt tgagttttca
     5401 cggcgtaccg cagaagcact acgacctcgg cgacccctat cccgacgagt gccgccacac
     5461 cgccaaactg cttgccgaag cactggaact gaccgaagac caatatgtcg tatcgttcca
     5521 aagccaattc ggcagggcaa aatgggttac accgagcacg caggatttgt tcggcaaact
     5581 gcccaaacag ggggtaaccg agctggacgt attctgcccg ggctttttgg cagactgttt
     5641 ggaaaccatg gaagaaatcg ccctgatggg gcgggaacag ttttacgaag caggcggcaa
     5701 aagctaccgc tacatcccct gcctcaacga caaccccgac tggatagacg cactcgtcgc
     5761 acttgccgaa gaaaaccttg gcggctggcg ttgaaactgt ttgacccatg tctaaaatgc
     5821 cgtctgaacg ccgtgttcag acggcatttt cccgctactt tagcgtatga tggcatctat
     5881 gccggacaac aacatcaagt gcaaccttac taccgattcg gcaaaaaaat ctttcacccg
     5941 ctacgcaaaa tcaacaacaa ggtttcaaac attaaggaaa caccaatgaa cacaacccga
     6001 ctgccgaccg ccctcgtctt gggctgcctc tgcgccgccg cttctgccgc cgacaacagc
     6061 atcatgacaa aagggcaaaa agtgtacgaa tccaactgcg tcgcctgcca cggcaaaaag
     6121 ggcgaaggcc gcggaaccat gtttccgccg ctctaccgct ccgacttcat catgaaaaaa
     6181 ccgcaggtgc tgctgcacag catggtcaaa ggcatcaacg gtacaatcaa agtcaacggc
     6241 aaaacctaca acggattcat gcccgcaacc gccatcagcg atgcggacat tgccgccgtc
     6301 gccacttata tcatgaacgc ctttgacaac ggcggcggaa gcgttaccga aaaagacgta
     6361 aaacaggcaa aaggcaaaaa aaactaaaca gacaaaatgc cgtctgaaac cggcaatccg
     6421 gcttcagacg gcattcaaat caaacctttc taacgcggta ctttcagccc catagacccg
     6481 atattgccgc caccgtcaac ctccttcgcg gtcagcataa agccgccgat gtcaatacgg
     6541 ccgcactcga ccggctcctg actgtcggaa cgcttatcga aaagctcggc aaggctcaaa
     6601 ccctcttcgc ccgcttccag cctcaaaccg taagcaagtg ccaaatcacc cgaacgtgct
     6661 gccggcgaaa cgacaaactc gccgaaaaaa tcaaaatgct cacggacacc gatacccgtc
     6721 tcggtaaagt atttcgccat tttatagtga a
//
LOCUS       DAWFAN010000103         6716 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWFAN010000103 DAWFAN010000000
VERSION     DAWFAN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.103"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(47..814)
                     /locus_tag="WGO65_001461"
     CDS             complement(47..814)
                     /locus_tag="WGO65_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0327745.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(902..1729)
                     /gene="mutM"
                     /locus_tag="WGO65_001462"
     CDS             complement(902..1729)
                     /gene="mutM"
                     /locus_tag="WGO65_001462"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ0327746.1"
                     /translation="MPELPEVETTLRGIAPRIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPRCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1781..2446)
                     /locus_tag="WGO65_001463"
     CDS             complement(1781..2446)
                     /locus_tag="WGO65_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEZ0327747.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGRWL
                     SLSEDVVCVPRDMSMSAENMALADVSVDMLLFPHTLEGGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPEWDGYLGGKALAAGAAR
                     VAD"
     gene            2625..4601
                     /locus_tag="WGO65_001464"
     CDS             2625..4601
                     /locus_tag="WGO65_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0327748.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDIYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIVRLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSSQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4669..5361
                     /locus_tag="WGO65_001465"
     CDS             4669..5361
                     /locus_tag="WGO65_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEZ0327749.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            5858..6460
                     /locus_tag="WGO65_001466"
     CDS             5858..6460
                     /locus_tag="WGO65_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0327750.1"
                     /translation="MPTLSQGLPNNYIYRENGVEFGLVQIRYPEHIELLHRWMNMPHV
                     IPQWQLNKSLSELSVYFEKMLADDHQRLYLVSINGEWVGYAEVYEGARDRLSGYYTAD
                     KNDLGWHLLLAEAQAVGKGYLRAIMRLLSFFIFEHSPAKKIVGEPDASVKPYEVVAQE
                     LNYMPQYQIVMPEKTAMLYFCDKEGFYCKFAHYLNASIKE"
     gene            6465..>6716
                     /locus_tag="WGO65_001467"
     CDS             6465..>6716
                     /locus_tag="WGO65_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327751.1"
                     /translation="MPIFLNAIQNPNSSLPVFLLDFSGEISQDAFDDWLIAMSDLLAS
                     EQSFALIYHSRTGLTLPNNYRQIEAVWYKTHKESFFKYCK"
BASE COUNT         1831 a         1975 c         1427 g         1483 t
ORIGIN      
        1 tacatccaca aaagcgaatc atactgccac attccgacaa ccggtttcag acggcaatat
       61 ccgcatcgtc ggcgacaacg gcacggatgc tttcttcgat tttatctttt aaagcataac
      121 ggtcttcgct ttccgccgcg tccgccacgc aaacgaaatc gacttttatc gttaattttt
      181 tcatagacac aatgcgccac agacaggtcg gcaaaccgac atcggcatat gagggacgag
      241 ccgtcctttt tcccgtttcg tcataataac gcagcgcgac cgccaaaacc tttgcccccg
      301 catcaatggc ggattggaac agcgcggctt tgaacggcaa aagccccaat ccggaggaag
      361 tccgcgcttc ggggaaaaaa ctgacgtttt gaccgcgttg caaggtttcg cagacggcgc
      421 ggttaatcgg ttcgatgtcg cgccgcgaat tgcggttgat gaacaccgtt cccgcgttct
      481 gccccatctt gcccaatacc ggccagcttt tgatttcctg cttggcgata aagctgctcg
      541 gataaaccgc gctcatcgcg aaaatatcca gccaggacac gtggttggcg gcaaccaaga
      601 caccgttcgg atgttcgggt gcgggtctgc ccacctccaa tccgatatcc aaagccgcca
      661 aaacccccct gcccaactcg attaccgccc gattgcgcga ctcggggcaa ccgccgtcaa
      721 taccgcgcag gtttttcccg gttttgaaca gccagaccgc caaacggcac aagcggcgca
      781 gacgtgtaaa aaatgaagct ttatttgaag acatgagcaa ttctttcccg ataatcggtg
      841 tttgttgttg aaacgttcgg acggcatacc gataaaatgc cgtctgaaac cgttttcagt
      901 cctatttctg acagttcggg caataaaacg tgccgcgctg ccccaaagtt tctttcacaa
      961 ccaaaccgcc gcaccggggg cacggctgat tgtgccgccc gtacactgta tattcctgtt
     1021 ggaagtagcc gcttttgccg tcgctgtcca caaaatccct caaggtactg ccgcccgttt
     1081 caatggcgcg ctgcaacacc gctttgacgg tttcaaccaa aagcgcgcac tctttctttt
     1141 tcaggcggtt ggcaggacgg tggggcgaaa tgcccgctct gaacaggctc tcgttggcat
     1201 aaatgttgcc cacaccgacc acgaccgcat tgtccatcag ggcaagtttg accgcgcgct
     1261 tctgcgcctt cagccttgca tacagataat ccgcacaaaa tgcctccgac aaaggctccg
     1321 gccccagttt ttccaacagc ggatgatgtt cttcgattcc ctcataccaa agtatcgcgc
     1381 cgaactttct cggatcgcgg taacgcatga ccgtgccgtc tgaaaacaca atatcgacgt
     1441 gatcgtgtct gtccggcctg ccgatacgtc cgtccgacgg cgtaaaaatc cgcaagctgc
     1501 ccgacatccc caagtgaatc agcaaaatcc ccgtttggaa acggacaatc agatacttcg
     1561 cccgcctgcc gcaggacaac acctgccggc cggacaaaat ctcccccaaa tcgggattaa
     1621 tctgccagcg cagcttcaat tggcgcaata ccacggcttc caccgttttc ccttcaatac
     1681 gcggcgcgat gccgcgcaac gtcgtttcca cttccggcaa ttcaggcata acccctcccg
     1741 acatttcttc tgacagatgc cgtctgaaag acggctgtcc ctaatccgca acccttgccg
     1801 cacccgccgc aagggctttg ccgcccaaat acccgtccca ctcgggcagg ggcgttacgc
     1861 ccgccgccct tttgaccaaa accaaaccgt acactgcggc acactgcggc caccaacggt
     1921 cgcccgcctt ttccataaac cgccaaaagc gtatttgccc gagcgacgaa acgggcggca
     1981 gatacaccat aaatttccca aattcaatat cgaaaccggc atcggcaagc cgccttttca
     2041 actcgggcaa cggcagacaa aaccgttttt ccggcaggcg ttcgccgtca aaccaacggc
     2101 tgaatcccca gagcgaatac ggattgaaac ccgtcagcat caaacgtccg gacggtttca
     2161 atatccggtg cgcctcggac aggatttgcg aaggaacacc gccttccaaa gtatgcggga
     2221 aaagcagcat atccacagaa acatccgcca aagccatatt ttccgccgac atcgacatat
     2281 ctcgcggcac acagacaaca tcttcagaca ggctcagcca cctgccgccc acctgaaccg
     2341 cacacattcc cgaaaaacgg tatgaatcca gataccgccc gaagaaatcc tgttccaatt
     2401 ttgcaacata ccgccccatc gccgtatctt caaaccatgc atccatattg cgtccgtttc
     2461 aaacagattg cctgccgata ttctattcca aacaggattt ctgtcaaaaa acacatcggc
     2521 cgcccatttc cgaatccgca taaagttcct gtaaaacttg acgctttttc agtcaaacag
     2581 taccatcgga cgataaaata tgtttattcc gccgaatata aatcatgtcc aaactcaaaa
     2641 ccatcgctct gaccgcatca ggtctgtccg tttgtccggg tttcctatac gcccaaaaca
     2701 cctcatcaca ccaaatcggt ttggcgatta tgcgcttaaa ctcttcaata ctcgacctgc
     2761 ccccgacaaa acaatatttc caatccggca gcctgtggag cgagctgcgc caaggcttcc
     2821 ggatgggcga agtcaatccc gaactggtac gccgccacga aagcaaattc atcgcaagcc
     2881 acagctattt caacagggtc atcaaccgga gtagacccta tatgtaccat atcgccaacg
     2941 aagtcaaaaa acgcaatatg cccgccgaag ccgccctgct tcccttcatc gaaagcgcgt
     3001 tcgtcaccaa agccaaatca cacgtcggcg catcaggatt atggcagttt atgcccgcta
     3061 ccggcaggca ttacggcctg gaaaaaacac cggtttacga cggcaggcac gacatttacg
     3121 ccgccaccga tgccgcactc aactatctgc aatacctcta tggactgttc ggcgactggc
     3181 cgctcgcctt tgccgcctac aactggggtg aaggcaacgt cggacgcgcc atcaaccgcg
     3241 cccgcgccca agggctcgaa ccgacctacg aaaacctgcg tatgcccaac gaaacgcgca
     3301 actatgtccc caagctgctc gccgtgcgca acattattgc cactccccaa tctttcggca
     3361 tgaatatcag cgacatagac aacaaaccct attttcaggc agtcgaaccg gatcgtccgc
     3421 tcgacaacga agccatcgtc cggcttgccg gcatcacgca aagcgagctg ctcgccctaa
     3481 accccgcatt caacgtcccc gcgtttatcc ccaaaagcaa acgcaaactg ctgcttcctg
     3541 tcgcgtccgt acaaaccttc caaagcaact acctcaacgc cgcacccgac agcctgtttt
     3601 catgggaagt ctatacgcct gccgccaaaa ccagcctgtc cgacatctcg acggcaaccg
     3661 gcatgagcat tgccgacatc aaacgcctca acaacctgaa cggcaacctt gtcaacgcag
     3721 gacgcagcat ccttgtcgcc aagaacggca aaacccttca gacggcatcg gaatccgtcg
     3781 tttccatcga catcgacaat acgcccgaca cctaccgttc caatatgccg gcaggcacgg
     3841 tgaacgtcgg cattgcccga atccgacccg ccgccgcaca gacagcggac attaccgtcg
     3901 cacctttgcc gcagaaaacc gtccgtacgg aacccgatcc ccttgtccgc attgccgaac
     3961 ctgcccttgc gacagccgca gcgcaacctc aaaccgaaaa acagaccgcc atgccgtctg
     4021 aaacccaaac cgcaacactc gcgcagatca tcccccaaaa cgacatgcag gcggcagacg
     4081 aactcatgca gcttgttgcc cgaaacaacc tgcgccgaca ggctgaagaa accatctccg
     4141 ccgtcatcgg cacgcctgac acagttgccg aacacaacat ttcctcatct tcgcaacata
     4201 ccgttgccgc cgacggcaaa cgccgggcac gtttggaaac gcgcgtagcc aaagctgccg
     4261 acggcgaagc cgaaatctcc ccgctccatg ccagcatcca ccgcgttgta gaaggcgaca
     4321 ccctgttcaa cattgccaaa cgctacaacg tcagtgtagc cgacctgatt gtcgccaaca
     4381 acatcaaagg caacaccatc caaaaaggac aggtactccg cctggcgcag gcagcccctg
     4441 cccaaacccg tattgaaaaa gtatcctaca ccgcgcgcaa aggcgacacc ttcaaaagta
     4501 tcgccgcgcg cttcaatatc catatcgacg acatccgccg gctcaatccc aacctgaaca
     4561 ccatcaatcc aggacagagg gttaaactga ttggaagctg attcggatac ggcacatgac
     4621 aggactttct cagtcctgtc tttttcatcc cacatttccc ataccatcat gaaactcatc
     4681 aaatacctgc aatatcaagg cataggaagc cgaaagcagt gccaatggct gattaggggc
     4741 ggctacgtct ccatcaacgg aacctgtatg gacgacaccg atgcagacat cgattcctca
     4801 tccgtcgaaa cgttggatat tgacggggaa gcagtaaccg tcgttcccga accctatttc
     4861 tacatcatgc tcaacaagcc tgaagattac gaaacttcgc acaaacccaa gcactaccgc
     4921 agcgtattca gcctgttccc cgacaatatg cggaacatcg atatgcaggc ggtcggcagg
     4981 ctggatgcgg atacgaccgg cgtattgctg attaccaacg acggcaaact gaaccacaac
     5041 ctgacttcgc cgagcagaaa aattcccaag ctatacgaag taacgctcaa acaccccaca
     5101 ggagaaacgc tctgcgaaac cttgaaaaac ggcgtgctgc tccacgacga aaacgaaacc
     5161 gtttgcgccg ctgatgccgt tttggaaaac ccgaccaccc tgctgctgac cattaccgaa
     5221 gggaaatacc accaagtcaa acgcatgatc gccgccgccg gcaaccgcgt gcaacacctt
     5281 catcgccggc gattcgcaca tctggaaaca gaaaacctca aacccgggga atggaaattt
     5341 atcgaatgtc caaaattctg aaataacatc caagaattcc atttatattc atccacatac
     5401 tcattaaata tatggtttaa cccaatttaa cgcaaaaaat caattaatga gttatcaaag
     5461 attattgatg aatttcacca tcaacatcca gagctaaatg agaggattgc tttatttgat
     5521 ttaaaaagac caaaatttga aaaggtgtgc ctaaatcgtg ttcgcttttt tacaagaggt
     5581 tatcaagata acgccaaccg tccagaaccc gtggtttgcg aaccaatttg caatccaata
     5641 tcaccacagt ttctaaggtg tgttgaacat tgataacatt atttgtaaaa atgatgaaaa
     5701 ttttaacgct tttattagtt tttgcatgaa aaacgtgcga tttttctcat ttttcattgc
     5761 aaataccaaa tattcaacac accctaatga gagaatagaa atgctagaaa tctataccga
     5821 ttcttttaat caagatcaat gcagtaaaat caatcgattg ccaactttat ctcaggggtt
     5881 gccaaacaac tatatttaca gagaaaatgg ggttgaattt gggttggtgc aaatacgcta
     5941 ccctgaacat attgaattgt tgcatcgttg gatgaatatg ccccatgtta tccctcaatg
     6001 gcaactcaat aaatcattgt ctgagctgtc tgtttatttt gaaaaaatgt tggctgatga
     6061 tcaccagcgt ttgtatttgg ttagtattaa tggcgaatgg gttggttacg ccgaagttta
     6121 tgaaggggca agggatagac tatcagggta ttatacagct gacaaaaatg atttgggttg
     6181 gcatttgtta ctagcagagg cacaggctgt tggcaaaggg tacttgcgtg ccatcatgcg
     6241 tctactaagc ttttttattt ttgaacattc acctgctaaa aaaattgtgg gtgaacctga
     6301 tgcaagtgta aaaccctatg aggtagtcgc tcaagaatta aactatatgc cccaatatca
     6361 aattgtaatg cctgaaaaaa ctgcaatgtt atatttttgt gacaaagaag ggttttattg
     6421 taagtttgcc cattatttaa atgccagcat taaagagtga tgttatgcct atttttctaa
     6481 atgctattca gaacccaaat agctccttac ctgtattttt actcgatttt tcaggcgaga
     6541 ttagtcaaga tgcatttgat gattggttga ttgctatgag tgatttgtta gccagtgagc
     6601 agagttttgc cttgatttac catagtcgga cagggctgac actccctaat aactaccgcc
     6661 agattgaggc ggtttggtac aagacgcaca aagaatcgtt ctttaaatat tgcaag
//
LOCUS       DAWFAN010000104         6678 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWFAN010000104 DAWFAN010000000
VERSION     DAWFAN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.104"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="WGO65_001468"
     CDS             complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="WGO65_001468"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ0327752.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(1292..2479)
                     /locus_tag="WGO65_001469"
     CDS             complement(1292..2479)
                     /locus_tag="WGO65_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ0327753.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(2472..3485)
                     /gene="dcm"
                     /locus_tag="WGO65_001470"
     CDS             complement(2472..3485)
                     /gene="dcm"
                     /locus_tag="WGO65_001470"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0327754.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(3543..5822)
                     /gene="nrdA"
                     /locus_tag="WGO65_001471"
     CDS             complement(3543..5822)
                     /gene="nrdA"
                     /locus_tag="WGO65_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ0327755.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTSQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPCVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            6351..6662
                     /locus_tag="WGO65_001472"
     CDS             6351..6662
                     /locus_tag="WGO65_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ0327756.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         1782 a         1493 c         1419 g         1984 t
ORIGIN      
        1 gaacaataac cgatcctacc cgctgttttt cccatcatac aacatacaaa tgccgtctga
       61 aacatccggc ttcagacggc attttttcaa aaaacgttta caactcaaaa tcgcccaaat
      121 catccgtatt cacttcagaa tcgatctgac cgatcaaata agaggatatt tccacttcct
      181 gcggcgcgac ctgtacgtta tcggacgaca gccacgcatt aatccacgga atcgggtttt
      241 gatttgcgcc ttcaaatccg gccggcaacc ccaccgcctg catacgcaga ttggtaatat
      301 attcgacgta ttgggataag atttctttgt tcaaaccgat catcgaaccg tctttaaaca
      361 aatatgccgc ccattctttt tcctgttccg ccgctttttt gaagagttgg aaacattcgt
      421 cctgcaactc agcggcaatt tctgccattt cagaatcatc gacacccgaa cgcatcagat
      481 taagcatatg ctgcgtgccg gtcaggtgca gggcttcgtc gcgggcaatc agtttgatga
      541 ttttggcgtt gccttccatc aactcgcgct cggcaaaagc aaacgagcag gcgaatgaaa
      601 cgtagaaacg gatggcttcc aacacgttga cgcacatcag gcagagatag agttttttct
      661 tcaacccgcg caaagacaca gtaatgggtt ttccgccgac attgtgcacc ccttcgccca
      721 acagattgta atactgggtg tattcgatta agtcatcgta atagcaggca atgtcttcgg
      781 cgcgggcggt aatgtattcg ttttcgacaa tatcatcaaa cacgaccgac ggatcattca
      841 caatattgcg gatgatgtgg gtatagctgc gcgagtggat ggtttcgctg aagctccacg
      901 tttcaatcca cgtttccaac tcgggaatcg aaaccaaagg cagcaaagca acattcggac
      961 tgcgcccttg gatggaatcg agcagtgttt ggtatttcag attgctgatg aaaatatgtt
     1021 tttcgtgttc aggcaggttg gcgtagtcga tacggtcgcg cgacacgtcg atttcttccg
     1081 gccgccaaaa aaacgacaat tgtttttcaa tcagtttttc aaatacctcg tatttctgct
     1141 ggtcataacg ggcgacattc acaggctgac caaaaaacat cggctcattc agcgcgtcgt
     1201 ttttggtttt gggaaaggtg ctgtatgaca tgactaggtg ttcgcaggac ataatctatt
     1261 ctctatatta attaaaaatg atctgtaagt tttaactaac ctgctgagac tttatatatt
     1321 taataaaatt ttctaaagtt aaaacatggg ttttcccaaa agttccaaat gtttttacgg
     1381 attctttagg gacaactaag gttaaattct catcactcat ttcctgaatt tgagtatcag
     1441 atataccttc ttgtagagta aataaatgct tatgaggaat tcgatctgct tcatttaaaa
     1501 cttggcgcca gcgatcctta caagtagtct ttgaacctag cattgtcagc ttttcagttt
     1561 gaaatatgaa attccctgat tcatccatcg catggtactc ttcacttcca ggaaataaaa
     1621 aatctggttt ctttttccct tctgtcttcg cttgcgtctc aaattttaag gaaaattcgt
     1681 caaatattct tgataaatgt aactctaaag attttcctgc tcttgctttt ctgcgatttg
     1741 taaaagaatg tgcaaaatta atgaagctat ccaaatcagt aatttttgat ttaataattt
     1801 caaattcacg tttttcaaaa atggaaaata attcatattc ctttgaaacc cattgtttta
     1861 acacattact aacgttagtt ttatttaatg caatattttc ccttgccaaa actgccattt
     1921 cttcagtttt ggggaagtta ggaaatagtg aaaataattt tgttaggtta tcctctactt
     1981 cttgtttttt aggtaaatat aaacttcctg gtaaaatgtt agtttctgcg ataaatattt
     2041 caatatcttc atctgaagat aaaacaaaag catggaataa taaatcttta taacaagctc
     2101 tgcacaatac caataaggaa ccactatatt tatcacttaa aaattcaaaa ttcttgccaa
     2161 aacccgtaat tctcgcctca tttctcgtgc cttggccgta atatttaaaa ttgctattag
     2221 tgacgcttcc atcttgccaa ttaattttga ttgaaatagt tttatttgtt cccttttggc
     2281 aaactacatc aaagaggtcg tctgaaaaat gtttttgaat ataaaatcct gattggtgac
     2341 taccagtagt accaacatca ttgggacgaa tataacgaca gtatactgca attgatttat
     2401 ttgctgtctg tattgctgaa gctactaagt catcggattt atttactact tgtattgtct
     2461 gagctgccaa gtcattcatt ttttaactca tttataattt gtttagcaat agcttgaacc
     2521 aacggtacag caattgaatt gccaaactgc ttgtatgcag ctgtcttgga tactgcatca
     2581 ataacaaaat ctttaggaaa tcccattaaa cgcgagcact ccctaggtgt cagcttccta
     2641 ggattttttc ctttctgagg gatgagtatt tcggaaccat ctttgtaata tcgtgcagat
     2701 agagttcgtg atattccatc taaatcaact aatccaaaac caaatccatt accctttgcc
     2761 ttatgttttt tagcgtaatt ttgaaggtaa agccataagt tatcagaaag agtaaaagaa
     2821 ttatctacat catcttccaa aatttgcttt aattttggtt gtgattctgg tggggaagga
     2881 aaattgaaat ttatttcctt attaaaatat tgtctatcaa aacctacaat aaaaatacgc
     2941 tccctatttt gaggaacata atattttgca ttcataactt gataaaatat ctgatagtca
     3001 agctcttcta aagtcccttt aattacttta aatgtatttc ctttgtcatg cgaaacaagg
     3061 tttttcacat tctctaaaag aaaaatttta ggtcgatgtt ttccaataat ttcagcaaca
     3121 tcaaaaaata gagttccctg cgccttatct aagaagcctg tttctcgtcc taggcttttt
     3181 ttctttgaaa caccagctat agagaatggc tgacacggga atcctgctgt taatacatca
     3241 aacttacttg gaatagctgc tttggtttcc tttaatgtaa tatctccata aggaatatca
     3301 ttaaaattta cttggtaggt ttgacgggct ttatcatccc attcactaga aaatacacat
     3361 cgcccaccaa cattctccat tgcaatgcga aaaccaccta ttcccgcaaa taaatcaata
     3421 aaagtaaatt tctcattatt taaggtaact atattatcta attgattttg tattttattt
     3481 ttcatatttt atatttcaga tgatttatta atctaaaggc catctgaaaa ctcccccttt
     3541 catcaaatct tacaagcccc gcccgcgcag ccgtcatcct gaatatcggt ctgcgtatcg
     3601 tccgcaccgt cgcgggtgtt atggtagtac agggttttga cgccgtattt gtaggcggtc
     3661 agcaggtctt tgagcatttg tttcatagaa actttgccgc cttcgaattt gcccgggtcg
     3721 taggcggtat tggcggaaat cgattgatcg acgaattttt gcatcacgcc gacaagtttc
     3781 aggtagcctt cgttgccggg aagctgccac agggtttcat aggcattttt cagggtttca
     3841 aactccggca cgacttgttt caaaatgccg tctttcgatg ctttgaccgt taccaatccg
     3901 cgcggcggct cgatgccgtt ggtggcgttg gcgatttgcg aactggtttc agacggcatg
     3961 agcgcggtca gggtggagtt acgcaggccg tatttgacga tgtcggcacg caggctttcc
     4021 cagtcgtaat gcaaaggctc gccgcagacg gcatccaaat cttttttgta agtgtcgatg
     4081 ggcagtttgc cttgcgaata aacggtttgg ttaaagagcg tgcacgcacc gtattctttg
     4141 gcaaggtttg ccgatgcttt gagcaggtaa tactgtatgg cttcaaaggt acggtgggtc
     4201 agaccgagcg cggagccgtc gctgtagcgg acaccgtttt tcgccagata ataagcatag
     4261 ttaatcacgc cgatgccgag cgaacggcga cccatagtag aggtacgcgc ggcttctacc
     4321 ggatatccct gataatctaa aagtgcatcg agcgcacgca cggtcaagtc ggcaagccct
     4381 tccaattcgt ccaagctgtt taatgcgccc aagttaaagg cagacagtgt acacagggcg
     4441 atttcgccgt tcggatcgtt gatattgtcc agcggtttgg tcggcagggc gatttccata
     4501 cacaagttgg actgatgaac aggcgcgacg cacggatcga acgggctgtg cgtattgcag
     4561 tgatcgacgt tttgaatgta gatgcgcccg gtcccggcac gctcctgcat cagcgtggaa
     4621 aacaggtcgg cagccggaat gatacgcttg cgtatgttcg ggtcttgctc gtatttcgta
     4681 tagagccgct caaattcgtc ttggtcggca aaaaacgctt cgtacaaccc cggaacctcg
     4741 ttgggcgaaa acagcgtaat gttgccgccc ttaatcaggc gggtgtacag caggcggttg
     4801 atttgcacgc cgtaatcaag ctgacggata cggttgtctt ccacaccgcg gttgtttttc
     4861 aacaccagca ggctttcggc ttcgatatgc cacaaggggt agaacaaagt tgccgctccg
     4921 ccgcgcacgc cgccttgcga acaggatttg accgccgcct gaaacatttt gaaaaacgga
     4981 atgcagccgg tatgccgcgc ttcgccgccc cggatttcgc tgtccaaacc gcggatacgt
     5041 ccggcattga tgccgatgcc cgcacgctgg gaaacgtatt tcacaatcgc gctggtggtg
     5101 gcgttgatgg aatccaggct gtcgtcgcat tcgatcagta cgcagcttga gaactggcgc
     5161 gtaggcgtac gcacgccgct cataatcggg gtcggcagcg atactttaaa tgtggaaacg
     5221 gcatcgtaaa accgtttgac gtaatcgagg cgtgtttctt tgggatattt gctgaaaaga
     5281 cacatcgcca ccagaacata taagaactgc ggtgtttcat aaatctggct ggtaacgcgg
     5341 ttctgtacca gatatttgcc ttcgagctgt ttgacagcgg cataggaaaa ggacatatcg
     5401 cgttcgtggt cgatataggc gttcagttca tcaaattctt cgcggctgta atcctcaagg
     5461 atatgcctgt cgtattttcc ggcatcggtg agctttttaa cgtggtcgta aaggtgcggc
     5521 ggctcgtatt cgccgtaggc tattttacgc agatggaaaa tcgccaaacg cgcggcaagg
     5581 tattggtagt ccggagtatc ttccgaaatt aaatcggcag cggccttgat gatggtttcg
     5641 tggatgtcgt cggtgcggat gccgttgtag aactggatgt gcgatttcaa ctcgacctgc
     5701 gacacggaaa cattttccaa tccttccgcc gcccaagtaa cgacacggtg aatcttatcc
     5761 aaatcaatgg cttctaatct tccgtctcgt ttggttactt tcaaatcagt cggtgtattc
     5821 atcgcttcct cttccactct tgatattcaa gacacggtct tttcaaacaa attaagacag
     5881 acaatatagt ggatttttgg cattttctcc agtcttgaca acggttgcat ttttcagttt
     5941 caggcaatgc gcgataaaac ccgcctgtcg tatttttcct ataatatttg ttttatctga
     6001 taattcttta ccgataaaaa acgggtaaat tttttgcctt ttgaccggct ccggctacaa
     6061 ggcggtgaaa taaggatttt ccgacgaaaa aggaaagctt ccggttttct gcccctgcga
     6121 attgttaaat tttgcaaagt atgattttgc cgcgccgccg ccgacaaatt ccattttctt
     6181 accgattgaa atttattatt gagattaatg tgttatttga atccgcatat caaacggcaa
     6241 gttttgtcgg ctgaatgagt ctgaaactgc cgacaatttg cccgttccat ccttccatcc
     6301 tatactggaa ggaatgacaa actgaaagga tcgtcatatc tgtcaaaaag atgcttgcca
     6361 tactgttgtc tgcaatattg ggactggtat cgacaactgc cgctgccggt acgtcagaac
     6421 ccgcccaccg ccataccaaa catatccgca aggcaaacaa gcagatgctg caccccgaat
     6481 gcaggaaata tttggaacgc cgtgccgcgt ggtaccgatc gcaaggcaac gtgcaggaat
     6541 tgcgcgaaaa caaaaaggcg cgcaaagcat tccgctccct gccttatgcg gaacagaaaa
     6601 tccaatgccg ggcggcttat gaggctttcg atgatttcga cggcggcagt ttccgccgtt
     6661 aatcccatat aaaatatg
//
LOCUS       DAWFAN010000105         6643 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWFAN010000105 DAWFAN010000000
VERSION     DAWFAN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.105"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            716..2164
                     /gene="gnd"
                     /locus_tag="WGO65_001473"
     CDS             716..2164
                     /gene="gnd"
                     /locus_tag="WGO65_001473"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ0327757.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFIEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2227..3498
                     /gene="waaA"
                     /locus_tag="WGO65_001474"
     CDS             2227..3498
                     /gene="waaA"
                     /locus_tag="WGO65_001474"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ0327758.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRGERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGVLMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3539..3988
                     /locus_tag="WGO65_001475"
     CDS             3539..3988
                     /locus_tag="WGO65_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ0327759.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            4021..4995
                     /locus_tag="WGO65_001476"
     CDS             4021..4995
                     /locus_tag="WGO65_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0327760.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFKLAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFVFLKYGLA
                     FVLGFIGVKMLIMHWVHIPISVSLSVVFGALGASVLTSLVYTKKQSDK"
     gene            5064..5139
                     /locus_tag="WGO65_001477"
     tRNA            5064..5139
                     /locus_tag="WGO65_001477"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5097..5099,aa:Lys,seq:ttt)
     gene            5358..6611
                     /gene="murA"
                     /locus_tag="WGO65_001478"
     CDS             5358..6611
                     /gene="murA"
                     /locus_tag="WGO65_001478"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ0327761.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMVVEGA
                     GELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
BASE COUNT         1522 a         1616 c         1928 g         1577 t
ORIGIN      
        1 agagcggttt ctgttttttc cgataaatgc cgcaatctca aatcccgtca ttcccacgaa
       61 agtgggaatg acggaatgta ggttcgtggg aatgacgcgg tacaggtttc cgttacgtat
      121 ccccatccga aagtttattt atacacaagc aatccaaagt tatctgactg aaatattgtg
      181 agatttaccc gcaaatctca ttttttccac cacttataca caaattcgga tgcgcactct
      241 gccgatttgc agaaataaga cattcgtgcc gacacctgac catttccaca agttccaaac
      301 acgcttccgg cttaaatctc cacagcattc ccgttcttaa aggcaccggc agccattcgg
      361 taaagaaaag taggatgcca tctgcacaac aagcaaacta tccacaaaac ttattcccca
      421 taaaaaacaa tccacaggta tgcacaaaag ggaatccgcc ttaccgacac cctaaaaaaa
      481 ggtaatcctg atgattttta aaataaaaaa aagttaccca cagaaaattt ctcctatcaa
      541 tataatcatc gtttttttat ttaattttta tattttattt ataagccttc agggaaacgc
      601 acggtaaagc gtaaaggcat cttttcaaaa aattaaaaag ttaaggattg ttaaacaagt
      661 gtaaatcagg tagagttgcg gtcgataaat taaatctata acggagcttc ctgcaatgaa
      721 aggcgatatt ggcgtaatcg gtttggcggt tatggggcaa aacctgattt tgaatatgaa
      781 cgattgcgga tttaaggtgg ttgcctacaa ccgaacaatc ggtaaggtgg acgaattttt
      841 aaacggcgcg gctaagggaa cggatattgt cggcgcgtat tccctgcaag acttggtcga
      901 taagctggca aaaccgagaa aaatcatgat gatggttcgc gcaggttcgg tcgtcgacga
      961 ctttatcgaa caactgcttc cgttgttgga agaaggcgac atcattatcg acggcggcaa
     1021 tgccaattac cccgacacga cacggcggac acattacctt gccgaaaaag ggattttgtt
     1081 tgtcggggcg ggcgtatccg gcggcgaaga aggcgcgcgg cacggcccgt ccattatgcc
     1141 gggcggggat aagcgcgctt gggaagccgt caaaccgatt tttcaggcga ttgccgccaa
     1201 aacgccgcag ggcgaaccgt gttgtgactg ggtcggcagg gacggcgcgg ggcattttgt
     1261 caaaatggtg cataacggca tcgaatacgg cgatatgcag ttgatttgcg aagcgtacca
     1321 gtttatgaaa gacggcttgg ggctgtctta cgacgaaatg caccgcgtgt ttgccgagtg
     1381 gaacaaaacc gagctggatt cttatctgat tgaaatcact gcggcaattt tgggctataa
     1441 ggacgaaggc ggcgaaccgc tggtcgagaa aatcctcgat acggcagggc aaaaaggcac
     1501 gggcaaatgg acgggcatca atgctttgga tttgggtatt ccgctgacgc tgatttccga
     1561 agcggtattc gcccgctgcg tttcgtcgtt catagaacag cgcgtgcaga ccggcaagct
     1621 gttcgcccga accgttacgc ccgtcgaagg cggcaaacaa gaatgggtcg aggcgttgag
     1681 acaggccctg cttgcatcta aaatcatttc ctacgcacaa ggctttatgc tgatccgcga
     1741 agcgggcgaa agctacggct gggatttgga ctacggcaac actgcgctgc tgtggcgcga
     1801 ggggtgcatc attcgcagcg cattcttaag caatatccgc gacgcgtatg aaaacaatcc
     1861 cgatttggtg ttcttgggtg cggatccgta tttcaaaaat attttggaaa actgcctgcc
     1921 ggcgtggcgc aaggtggttg ccaaggcggt cgaatgcggc atccctatgc cctgcatggc
     1981 atcggcgatt acgttcttgg acggctacac gaccgaacgt ctgcctgcca acctcttgca
     2041 ggcacagcgc gactacttcg gcgcgcacac gtacgaacgc accgacaaac cgcgcggcga
     2101 gtttttccat accaactgga cgggcaaggg cggagatacc gcttcgacaa cctacgatat
     2161 ttgattccgt tgccgaaatg ccgtccgaac cgttcggacg gcatttgttt tgcaggaaat
     2221 agaaagatgt tccaatggct ttatgatgta ttgtggctgc ttgcgccgat atggatacgg
     2281 cgttatttgg acaaacgctc cggaagtgcc ccggcatatc gggcgcatcg gggcgagcgt
     2341 ttcggcaagc cgtatccgaa tcccgttacc ggcgcggttt ggatacacgc cgtttcggtc
     2401 ggggaaacgc gtgccgccca acctttgata cgcgagttgc ggcagcgttt tcccgatgcg
     2461 ccgctgctga tgacgcagat gaccccgacg ggacgggaaa ccgcgcaagt tctgtttccc
     2521 gatgcgcaat gccgctatct tccgtatgac aaaaaaacgt gggtacggca gtttttgcgc
     2581 gaacaccgcc cgatgttcgg cgtattgatg gaaacggaaa tctggcccaa cctaatgaag
     2641 gaatgccggc gcgcgggtgt gccgctgttt ttggcgaatg cgcggctgtc ggaaaaatcg
     2701 ttgaacggtt atctgaaagt ccgccgcctg atccgtcctg ccgccgcttc gctgacgggt
     2761 tgtctggcgc agacggaggc ggatgcggcg cggttggcga aattgggcgc ggcatccgtg
     2821 caggtgtgcg gcaataccaa atacgacctg atgccgtcgg aacagatgaa aacgctggcg
     2881 gggcagtttg aaaaacgcat cggaggccgg ccggttgccg tgtgcggcag cacgcgcgtt
     2941 tatcggggcg aagacgaggc ggaaaaactg ctggcggcgt ggcaacaata tcgcggcgat
     3001 gcgctgctgg tcgtcgtgcc gcgccatccc gagcattttc agacggtatt tgaaacggca
     3061 aaacgcttcg ggtttaaggt tcagcggcgc agcgacggtt tgccggtcga acctgatacg
     3121 caggtgtgga taggcgacag tatgggcgag ctgtatgcgt attacctgtg cgccgatgtt
     3181 gcttttgtcg gcggcagtct ggtcgattcg ggctgtcaga acatcatcga accgctttcc
     3241 tgcggcgttc cgacgatatt cggcttttcg acctacaatt tttccgaagc ctgccggcac
     3301 gccttggcat cgggtgcggc ggttcaagtc gaatcggcgg atgcgtggcg tgaagccgtt
     3361 gaaaaaacct tatcgtccga ggggggcgga atgcagatgc aggcgcgcgt ggacggcttt
     3421 atcgcacaac atcgcggagc gggcgcgaga atcgccgagg cggtgcggga agcggtatgc
     3481 ggacatcggg ggcgatagtg atacaatccg tatccgagtt ttccggttgg agtaccgtat
     3541 gagtatttat gccgtcgcgc acatcgtcca cctgtattgc gccattgcct ttgtcggcgg
     3601 cgtgtttttt gaagtgctgg ttttgtccgt cctgcatacg ggacgggtgt cgcgcgaggc
     3661 gcggcgcgaa gtggaaaagg caatgtctta ccgcgccgtc agggtgatgc cgtttgtggt
     3721 cggactgctg ttcgccagcg gcatcgtgat ggcggcaaac cgctatcttt ctatattggg
     3781 cgaaccgttt gccacttcct tcggtacgat gctgacgctg aaaatcctgt tggcgttcag
     3841 cgtgttggcg cacttcgcca tcgccgtcgt caaaatggcg cgttccacac tgaccgtcgg
     3901 ctggtcgaaa tacatacaca ccgtcgtctt tacccatatg ctgctgattg tctttttggc
     3961 aaaagcgatg ttttatatca gctggtaaca caactacatt tcaaatcagg agattgaacc
     4021 atgacagaat atcccggcat cgggtcgccg ttgttttacg gcgttttttt tgcggcagtg
     4081 ctggtcatga ttgccttgga tatgttttcg ctgaagaaaa acagcagcca taaagtcggc
     4141 gtcaaagaag ccttggcatg gagcggcttg tgggttgccg tatcctgcct gttcgcaggc
     4201 tggctgtatt tcaaactcgc cggcaatccc ggctacggcg cggcagtcgc aaaagaaaaa
     4261 gtattggaat tctttaccgg ctacattttg gaaaaatcgt tggcagtcga caatattttc
     4321 gtgttcctga tgatattcgg ctacttcaaa gtcgcaccgc agtttcagca ccgcgtgctg
     4381 ctgtacggcg tattgggcgc attggtattg cgcactgtca tgattttcgt cggcgcggca
     4441 ctggttcagc agttcgagtg gattctgtat ctgttcggcg cgttcctgct ctataccggc
     4501 atacacatga tgaagcccga aggcgatgag gaaggggatt tggcaaacag caggctgctg
     4561 aatgccgtca agaaagtcgt tccggtcggc acggaatttc acggcgagaa attttttact
     4621 gtcgaaaacg gtaaaaaaat cgccacgccg ctgtttttgg tgctggtcat gattgaattg
     4681 agcgatgtcg tttttgccgt ggacagcatc cccgccgtct ttgccgttac caccgatccg
     4741 tttatcgtgc tgacttccaa tattttcgca attctgggct tgcgggcgat gtatttcctg
     4801 ctggcggatg tggcggaacg cttcgtattc ctgaaatacg gcttggcatt cgtgttgggt
     4861 tttatcggtg tgaaaatgct gataatgcat tgggtgcata tcccgatttc cgtttcgctg
     4921 tcggtcgtgt tcggcgcatt gggtgcatcg gtgttgactt cattggttta tacgaaaaaa
     4981 cagtccgata aataaaatgc cgtctcaacg ccgacaaaat ggttgacagc atcaggcttg
     5041 atgtatagaa tttcgagctt gtcgggtcgt tagctcagtc ggtagagcag cggactttta
     5101 atccgttggt cgagcgttcg aatcgctcac gacccaccag ataaaacaaa aaagctgagt
     5161 tttcaaaact cggctttttt tgttttccga caatccttgt tcggaaagcg cggcatttcg
     5221 tcaaattctt tggtgcggct tccgtatttt tcccgaaaat cccttataat tccgcctctg
     5281 caaccttcat cgggtgatgc tttatgcgtt gtcccgagta ttgaaatatt acaagtaccc
     5341 accaggaaag aacaaacgtg gacaaactga aaatctccgc aaacggcccg ctcaacgggg
     5401 aaataacggt ctcgggcgcg aaaaacgcgg cattgccgct gatgtgcgcg ggtttgctga
     5461 catcgggtac gttgcgcctg aaaaacgtcc ctatgctggc agatgtgaaa accacgcaaa
     5521 agctgcttca ggggatgggc gcgcgcgtcc tgactgacaa tatcagcgaa tttgaaatca
     5581 acggcggtac ggtaaacaat acctgcgccc cttacgaatt ggtacggacg atgcgggcct
     5641 cgattttggt gctggggcct actttggcac gtttcggcga ggcgcaagtc agcctgccgg
     5701 gcggctgcgc catcggttcg cgccccgtcg atcagcattt gaaaggcttg gaagcgatgg
     5761 gtgctgagat tgttatcgaa cacggttacg tcaaagccaa aggcaaactc aaaggtacgc
     5821 gcgtggcgat ggatgtcgtt accgtcggcg gcacggaaaa cctgctgatg gcggcgacgc
     5881 tggcggaagg tacgacggtt ttggaaaact gcgccattga gcctgaagtg gtcgatttgg
     5941 cggaatgcct ggtcaaaatg ggcgcgaaaa tcagcggcat cggtacgtcc acgatggttg
     6001 tggaaggcgc gggcgagcta cacggctgcg aacacagcgt cgtccccgac cggattgaag
     6061 cggggacgtt tctgtgcgcg gtggcgataa ccggcggcag ggtggttttg cggaatgccg
     6121 ccccgaaaac gatggaagtg gtgttggaca aactggttga ggcaggtgcg gtgattgagg
     6181 cgggcgatga ttggatcgcc atcgatatgc ggcagcgtcc gaaggcggtg gacatccgca
     6241 cggtcgtcca ccccggcttc cccacggata tgcaggcgca gtttatggca ttgaatgccg
     6301 tggcggaggg aagctgccgc gtggtggaaa cgatttttga aaaccgcttt atgcacgtcc
     6361 ccgagttgaa ccggatgggg gcgaacatta caaccgaggg caatacggca tttgtgcagg
     6421 gtgtggaacg gctttccggc gcagtcgtca aggcgacgga tttgcgtgcg tccgcaagcc
     6481 tcgttatcgc cggtttggcg gcgcgcggcg aaaccgtggt cgaacagatt taccacttgg
     6541 atcgcggtta tgaaaatatt gaaaaaaaac tcggcagcgt cggcgcgaaa atcgagcgcg
     6601 tatccggctg atcgggttgc gatgccgtct gaagccttca gac
//
LOCUS       DAWFAN010000106         6578 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWFAN010000106 DAWFAN010000000
VERSION     DAWFAN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.106"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            176..1234
                     /gene="alr"
                     /locus_tag="WGO65_001479"
     CDS             176..1234
                     /gene="alr"
                     /locus_tag="WGO65_001479"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ0327762.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLIRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1298..2653)
                     /locus_tag="WGO65_001480"
     CDS             complement(1298..2653)
                     /locus_tag="WGO65_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ0327763.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(2668..2940)
                     /locus_tag="WGO65_001481"
     CDS             complement(2668..2940)
                     /locus_tag="WGO65_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ0327764.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3066..3803)
                     /locus_tag="WGO65_001482"
     CDS             complement(3066..3803)
                     /locus_tag="WGO65_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ0327765.1"
                     /translation="MKKLSRIVFSIVLLGFSASLSAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAYAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGSKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4084..4983)
                     /gene="prmB"
                     /locus_tag="WGO65_001483"
     CDS             complement(4084..4983)
                     /gene="prmB"
                     /locus_tag="WGO65_001483"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0327766.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5128..5721
                     /locus_tag="WGO65_001484"
     CDS             5128..5721
                     /locus_tag="WGO65_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327767.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            5833..6453
                     /locus_tag="WGO65_001485"
     CDS             5833..6453
                     /locus_tag="WGO65_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ0327768.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIATAAMKA
                     AIPDYMKFGEGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVAKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVDRPANPDAVSTCFKADKSAEAVNNDTEHNTA
                     YFSHFPSKQKSTDGGRCPFLYRFPYFLAAGRAGLAGPFGAGAPTEA"
     gene            complement(6384..>6578)
                     /locus_tag="WGO65_001486"
     CDS             complement(6384..>6578)
                     /locus_tag="WGO65_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEZ0327769.1"
                     /translation="GIGPAKAKAIAEYRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQA
                     SVGAPAPKGPAKPALPAAKK"
BASE COUNT         1778 a         1673 c         1515 g         1612 t
ORIGIN      
        1 atttgggaaa gcgattatat caggaaaagc aaaccgcctt cctacctaaa aactgctgct
       61 tcggcttgaa gatacaaggg tctttaatat tttaaaagcc ttgccgttgg attataattc
      121 cctaattgat ttcttaattt tgctaataaa cacttgtttg gtaaggaatg aatttatgcg
      181 ccctttgaac gtgcagatca ggttgggcaa ccttaggcac aattatcgga ttttgaagga
      241 aatgcacgga ggcaaactgt tggcggtagt gaaggccgac gcatacggac acggtgcggt
      301 cagatgtgct ttcgcgctgg cagacttggc agacggcttt gccgtggcga caatcgatga
      361 aggaatcagg ctgcgggaga gcggcattac ccatccgatt gtccttttgg aaggcgtatt
      421 tgaagcatcg gaatacgaag cggtcgaaca atactcgctt tggccggcag tcggaaacca
      481 atggcagctt gaggctttgc tgatccgcca ttggaaaaaa cccgtcaaag tctggttgaa
      541 aatggattcg gggatgcacc gtaccggttt tttccctcat gattacgctt cggcatatgc
      601 ggcattgaaa cagtcggaat atgtggacag tattgtcaag ttctcgcatt tctcctgtgc
      661 ggacgaaccc gaaagcggta tgacggaaat acagatggaa gcattcgatt tgggtacgaa
      721 agggctggaa ggcgaagaaa gccttgccaa ttcggcggct attttgaatg ttcccgaagc
      781 acgcagggat tgggggcgcg cgggcttggc gttatacggc atttccccgt tcggaggcag
      841 cgatgacagg ctgaaacccg tgatgaggct ttcaacccgt atttttggcg aacgcgtttt
      901 acagccgcac tcccctatcg gttatggcgc aacattttat accagcaaat ctacgcgcgt
      961 cggtctgatt gcctgcggtt atgcggacgg ttatccgcgc cgcgccccaa gcaattcccc
     1021 cgtcgccgtc gacggtaaat tgacccgggt catcggcagg gtctctatgg atatgatgac
     1081 catcgagctg gacgcttcgc aagaaggttt gggacacgag gtcgaactgt ggggcgatac
     1141 ggtcaacatc aatactgttg ccgaagcggc cggaaccatc ccttacgaat tgatgtgcaa
     1201 tatcaaacgt gcaaaattca cttatatcga gtaatcaagt ccaaacaaaa atgccgtctg
     1261 aagcctttca gacggcattt ccccatcaaa accgcaatca gtttttcatc gattggaccg
     1321 gagcgggaat tctgccgccc cgtttgacga acacttcgca cgaaccttct tttaccggca
     1381 tcacaggtgc gtagcccaac aggccgccaa actcgacgct gtcgccgacg gttttaccgg
     1441 ttaccggaat aatgcgcacg gcagtggttt tgctgttgat catgccgatg gcggcttcgt
     1501 cggcgatgat gccggaaatg gtatgagcag gcgtatcgcc gggaacggca atcatatcca
     1561 aaccaacgga gcagacagcg gtcatcgctt caagtttgtc caacgtcagc acaccggctt
     1621 cggcagcggc aatcatgccc tcgtcttctg aaacggggat aaacgcaccg ctcaaaccgc
     1681 cgaccgcgct ggaagccatc atgccgccct ttttcacggc atcgttcagc aatgccaaag
     1741 ccgccgtcgt gccgtgtgta ccgcagacgc tcaaacccat ttcttcaaga atgcgcgcca
     1801 ccgagtcgcc gacggcaggg gtcggtgcca acgacaagtc gagaatacca aacgggatat
     1861 tcagcatttt tgaggcttcg cggccgatga gttcacccac gcgggtaatt ttgaaagcag
     1921 ttttcttcac tacttccgca acttcggtca atgtcgttgc atctgaattt tccaacgcgg
     1981 ctttgaccac gcccggcccc gatacgccga cattaatcac agcatccgct tcgcctgagc
     2041 cgtgaaacgc gcccgccata aacggattgt cttccaccgc attgcagaac acgacgattt
     2101 tcgcgcagcc gaaaccttcg ggcgtaattt cagccgtgcg tttgatggtt tcgcctgcca
     2161 gcttgaccgc atccatattg ataccggcgc gcgtactgcc gacattgatg gagctgcaca
     2221 cgatgtcggt ggtcttcatc gcttcgggaa tggaacggat taacacctcg tctgaaggcg
     2281 acatgccttt ttgcaccaac gcggaaaagc cgccgataaa ggacacgccg atggctttgg
     2341 cagccttatc caaagtttgc gccacgctga cataggaatc ggcatgggta gccgccgcga
     2401 tttgggcaat cggcgtaacg gaaatgcgct gattcacaat cggcacgccg tatttggcag
     2461 acagatattt tgccgtcgcc accaagtctt tgccgaccgt ggtaattttg ttgtaaatgt
     2521 tttggttcaa cacattgata tcgctgctga tgcagtcgtg cagatcaatg ccgatggtaa
     2581 tggtgcggac atcaaaattc tggtcggcaa ccattttgac ggtttctaaa atttcgccgg
     2641 attggatact catcacattc ctccgactca aatgcggtgc atcgcttgga agatttcttc
     2701 attttgcata cggatatcaa gcgcgagttt tttgctctct tccgcaaaca aatccaaaac
     2761 ctcctgccga gatttggggc atttcgaggt atccaccaag ataatcatgg taaaaaaatc
     2821 atccatcagc tgttggctga tattgaggat gttgattcgg ttttccgcca aaattttgga
     2881 aacatcgtac acgatgccga cgcggtcttt accgatgacg gtgatgactg aattgttcac
     2941 aggcttactc cttgcagata tccgttaaag tccgaaatta taccaccgtt ggattttgaa
     3001 gaaatattgt caacaatata tacatacaaa atgccgtctg aaactatttc agacagcatc
     3061 aagattcagg gttcgattaa ataaccatcc ttatcccact gggttttcct gaccaacttg
     3121 tcatcctgat aaacagcttc actcttttta gaaccatctt cataccactc taagactact
     3181 ccattacgtt tatgatgatg gatagacagt tccgagagta atcgaccgct ttcatcccat
     3241 gtcagaatct tggccggctc atcattgacc ataaccattt ccgttttgat attgccatca
     3301 gcataccatt gtttccatac gccgtttgcc ttattttgtt taaactggat ttcgctttcc
     3361 ttgctgccat tacggtaata gcggtacccc gtaccttcac tcaaaccatt tttataaggc
     3421 ataaccgcag attttttacc gttcggatac cagttgaccc actcaccgtc cggcttaccc
     3481 ttgctgaagc cccccgccat ttttttctga ccattaaaat gccacaaaat caacataccg
     3541 ttttgcaggg taggcacaaa agatttgatt tgcgttgaag caacgatata aggttcggaa
     3601 tatttcttca tcgacggata ataaaaatcc tgcgcgtacg caataccctc cgccacacta
     3661 tattgcctga tataagcggc agaagacatc gtcgccgtca gtttcccgtt ctgattaaaa
     3721 taaacagaat aggtctgtgc cgataaagaa gccgaaaaac ccaacaggac aattgaaaat
     3781 acaatccgag ataatttttt cattgcaata gcgatataaa aacaaggctg tgttttagta
     3841 atctgttgat ttcaattatt tgcaagggaa aagacaatta ttttccggtt aggaataaac
     3901 ctattctact gaatatattg aagccaagta cgcctatcaa cactatatta aaacactgcc
     3961 aaaaacaatt aacttataaa caatatggta aggatttctc tgccaagcat caaacccgag
     4021 acaacgtatc gtaaaaatgc cgtctgaaaa caaatcgtct tcagacggca tttccccttc
     4081 aactcactct tcacccaata actgctcgcg cgtcaagagg aaaacaaaac cgtcgccccc
     4141 gctggtttcc aaccaagtaa aaggcaactc cggatacgct gcttccaata catccctgtt
     4201 atgcccgatt tccaccagca atacaccttt gggattcaga aactttgccg cattcagaag
     4261 aatctgcctg gtggcatcca acccgtccgc cccgctgccc aatgccaatt ccggttcgtg
     4321 caaatactct tcaggcaata actcaaccga ttccgcatcc acataaggcg gattggaaac
     4381 aatcaaatca taagtgcctt ccaatccttc aaacaaatcc gtatgaataa gccggatgcg
     4441 ttcttccaaa ccataatctt cgacattaat ccctgccact tccaaagcat ccaagctcac
     4501 atcaaccgca tcaatttggg catcaggata atgatgcgcc atctgaatgg caaggcaacc
     4561 gcttccggtg caaagatcca aagcattatg caccaactca tcgtattcta tccaaggacg
     4621 aagtccgtca cccaacaatt cataaataaa agaacgaggt atgattacgc gctcatccac
     4681 atagaaatca aactctccct gccatgcctg gtgtgtcaaa taagcggctg gaatgtgttc
     4741 gacagcacga cgctcaataa ccgtcagcac ttcctctttt tcagcttcca agagttttgc
     4801 atcaagatat ggggcaagca tatccaaagg caaattcaaa gtatgcagaa tcaaataagc
     4861 tgcttcatca tgcgcattat ccgttccatg accaaaaaag agccctgcct cattaaaacg
     4921 gctgactgca aaacgtaaaa catcgcggat agtcgtcaat tcttgtgctg cctgattaaa
     4981 cataatatga accattctgc gtatagatac ttttaattat aacagaaaca acaagcaaac
     5041 cttttcatat cgccaaataa ccacccaatc tacccataca actacataaa tgcccgcgcg
     5101 aaaaccatcg cccgaacgga aacgacaatg gccgacggta tgggcaacct gattggctgg
     5161 gaaaaaacgg ggcttgttgc cggtaagcag tggataaccg caaaagacga caaggtgtcc
     5221 gatgtctgca atgccaacgg cgagatgggc gtaatcgggc tttacgagcc tttctcacac
     5281 ggcgcattga cgatacccgg tcatccgaac tgccgatgcg aggttgtttc cgtatcgggt
     5341 ggcgaattgg gggaatttgc cgaaaaaaag gagcttcgta aagcggctat gcagtatgcg
     5401 cgggataact ttatcggcaa aagctatgtc aatgaaaaca gcgggcatga actgaaggta
     5461 acttggcaag gtgtgaaaca cgctgcgtca aaggcaaatc aggcggaatt atccatcatg
     5521 acaaaacttg atgacttatt gcgctacgca aaatatgagg gttcttattc ggataggaaa
     5581 ggtcatccta atattattgc agcacataag tatcgtgccg ttgccaaggt tgggaatgag
     5641 tctttaaata tcggtgtgat tgtaagggaa tttccagacg accataaaca ttacgaccat
     5701 ttcatcttga aggatgaata aagccctttt gcagtgtcgt tctggagcgg atagcgttaa
     5761 ggcaagtaca cttccagcct tgaaaaaggg ctttaaattc agcatgccat ttatacaggc
     5821 aggagtaaac ccatgacaaa gttatacgca gaaatcgcca agatggagac gcaggacgac
     5881 gacacggtca aggtttgggg ttacgcttca agcgaggaaa tcgattcgga cggcgaagtc
     5941 atcgcgacgg cagctatgaa ggcggcgatt cccgattata tgaagtttgg cgaggggcgc
     6001 gagatgcacg gctcaaacgc tgcgggaacg gcaattgaaa tcaacgttga agacgacggc
     6061 ataacctttt tcggtgctca tatcatcgac cccgttgtcg tggcgaaagt caaaacaggc
     6121 gtttacaaag gcttttccat cggcggcagc gttaccgccc gcgatgattt gaacaagtcc
     6181 caaatcacgg gcttgaagct gacggaaatc agccttgtcg accgccctgc caatcccgat
     6241 gcggtgtcta cctgctttaa ggcggacaaa agtgcggaag cggtaaacaa cgatacagaa
     6301 cataatactg catattttag ccatttccct tccaaacaaa aaagcaccga cggcggccga
     6361 tgccccttcc tttacaggtt cccctatttt ttagctgcgg gcagagccgg tttagctggg
     6421 ccttttggtg cgggcgcgcc gaccgaagcc tgatccttca gcttcgccag caccgcaggg
     6481 ccgatgccct tcaccttggt caaatcgtcc acagacttga acgcaccgtt ttgcgcacgg
     6541 tattccgcaa tggccttcgc cttcgccggg cctatgcc
//
LOCUS       DAWFAN010000107         6560 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWFAN010000107 DAWFAN010000000
VERSION     DAWFAN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.107"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            116..1657
                     /locus_tag="WGO65_001487"
     CDS             116..1657
                     /locus_tag="WGO65_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ0327770.1"
                     /translation="MDGRRWVVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGWQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRACSDKAVSPVMPSEPQSVGEYVLL
                     AFAAAVLGICCLFPLAAVVVKACSAGESWQVLAERETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTAYRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     QTETL"
     gene            1686..2285
                     /locus_tag="WGO65_001488"
     CDS             1686..2285
                     /locus_tag="WGO65_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ0327771.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVELIRRHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2282..2887
                     /gene="nadD"
                     /locus_tag="WGO65_001489"
     CDS             2282..2887
                     /gene="nadD"
                     /locus_tag="WGO65_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ0327772.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            2946..3332
                     /gene="rsfS"
                     /locus_tag="WGO65_001490"
     CDS             2946..3332
                     /gene="rsfS"
                     /locus_tag="WGO65_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ0327773.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3386..3856
                     /gene="rlmH"
                     /locus_tag="WGO65_001491"
     CDS             3386..3856
                     /gene="rlmH"
                     /locus_tag="WGO65_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ0327774.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAVSILHNHPYHRE"
     gene            3929..4195
                     /locus_tag="WGO65_001492"
     CDS             3929..4195
                     /locus_tag="WGO65_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ0327775.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4352..5035
                     /locus_tag="WGO65_001493"
     CDS             4352..5035
                     /locus_tag="WGO65_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ0327776.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5159..5671
                     /gene="coaD"
                     /locus_tag="WGO65_001494"
     CDS             5159..5671
                     /gene="coaD"
                     /locus_tag="WGO65_001494"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ0327777.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5703..6356
                     /locus_tag="WGO65_001495"
     CDS             5703..6356
                     /locus_tag="WGO65_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ0327778.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1448 a         1719 c         1884 g         1509 t
ORIGIN      
        1 ctatatttca gacggcggga ggaaggcgga tacggtatgt agtcggattg aaatatacgg
       61 tatgtggggg cttttgggta caatcgcaac atcagtgttt caagtgggga aggcgatgga
      121 tggacggcgt tgggtggtat ggggtgcttt tgccctgctg ccttcggctt ttttggcggt
      181 aatggtcgtt gcgcctttgt gggcggtggc ggcgtatgac ggtttggcgt ggcgcgcggt
      241 gctgtcggat gcctatatgc tcaaacgttt ggcgtggacg gtatttcagg cagcggcaac
      301 ctgtgtgctg gtgctgcctt tgggcgtgcc tgtcgcgtgg gtgctggcgc ggctggcgtt
      361 tccggggcgg gctttggtgc tgcgcctgct gatgctgccg tttgtgatgc ccacgttggt
      421 ggcgggcgtg ggcgtgctgg ctctgttcgg ggcggacggt ctgttgtggc gcggctggca
      481 ggatacgccg tatctgttgt tgtacggcaa tgtgtttttt aacctgcccg tgctggtcag
      541 ggcggcgtat caggggtttg tgcaagtgcc tgcggcacgg cttcagacgg cacggacgtt
      601 gggcgcgggg gcgtggcggc ggttttggga cattgaaatg cccgttttgc gcccgtggct
      661 tgccggcggc gtgtgccttg tctttttgta ttgtttttcc gggttcgggc tggcattgct
      721 gctgggcggc agccgttatg ccacagtcga agtggaaatt taccagttgg tcatgttcga
      781 actcgatatg gcggttgctt cggtgctggt gtggctggtg ttgggggtaa cggcggcggc
      841 agggttgctg tatgcgtggt tcggcagacg cgcgtgttcg gataaggcag tttctcctgt
      901 gatgccgtct gaaccgcagt cggtcgggga atatgtgctg ctggcgtttg cggcggcggt
      961 gttgggcata tgctgcctgt tcccgctggc ggcggtcgtg gtaaaggcct gttctgcggg
     1021 agaatcttgg caggtgttgg cggaacgcga aacttggcag gcggtgtgga atactttgcg
     1081 cttctcggcg gcggcggtgt ttgcggcggc ggttttgggt gtggtgtatg cggcggcggc
     1141 gcggcggttg gcgtggatgc gcgggctgat gtttttgccg tttatggtat cgccggtttg
     1201 tgtttcggca ggcgtgctgc tgctttatcc gcagtggacg gcttcgttgc ccttgctgct
     1261 ggcgatgtat gcgctgctgg cgtatccgtt tgtggcaaaa gatgttttgt cggcctggga
     1321 cgcactgccg ccggattacg gcagggcggc ggcgggtttg ggcgcaaacg gctttcagac
     1381 ggcataccgc atcacgttcc cccttttgaa accggcgttg cggcgcggtc tgaccttggc
     1441 agcggcaacc tgcgtgggcg agtttgcggc gacattgttc ctgtcgcgtc cggaatggca
     1501 gacgttgacg actttgattt atgcctattt ggggcgtgcg ggtgaggata attatgcgcg
     1561 ggcaatggtg ttgacattgc tgttgtcggc atttgcggtg tgcattttcc tgctgttgga
     1621 caacggcgaa ggcggaaaac agacggaaac gttataatgt gaaccctttt tcagaggacg
     1681 gcaaaatgag cgagttggat aacatccttg cccataaccg gcagtttgtc gagtcgggcg
     1741 aatatgaaaa atactttacc gacaaatacc ccgaacgcgg gctggcagtt ttgtcctgta
     1801 tggatgcgcg gattatcggg ctgctgcccg acgcgttggg tttgaaaaac ggcgatgcca
     1861 agctgattaa aaatgccggc gcgctggtta cgcacccgtg gggttcggtg atgcggagtc
     1921 ttttggttgc cgtgtttgaa ttgaaggtca gagagattat ggtcatcgcc catcacgatt
     1981 gcggtatgca ggggctgaat gccgaagaat tcctcgggcg cgtccgggaa agccagattc
     2041 ccgaagaccg tatcgaaacc ctgcgttatg ccggtatcga cctcgacggc tggctgaccg
     2101 gtttcgacaa cgtcgaagac agcgtgcgcc atactgtcga actcatccgc cgccatccgc
     2161 tgatgccgcg ccatattgcc gttcacggac tggtcatcca tcccgttacc ggcaaactga
     2221 cgctggttgt ggacggcagt gtttcagacg gcatggactt atcggaagga atggaaacat
     2281 catgaagaaa atcggactgt tcggcggcac tttcgacccg atacacaacg ggcatctcca
     2341 catcgcccgc gcctttgccg acgaaatcgg cttggacgcg gttgttttcc tgccggcagg
     2401 cggcccgtat cacaaagacg cagcctccgc ttccgccgcc gaccgccttg ccatggtcga
     2461 actggcgacg gcagaagacg cgcgttttgc cgtcagcgat tgcgacatcg tccgagaagg
     2521 tgcaacctat acttttgata ccgtccaaat cttccgccag cagttcccat ccgcgcaact
     2581 ctggtggctg atgggcagcg acagcctgat gaagctgcac acatggaaaa aatggcagat
     2641 gctcgtgcgc gaaaccaata tcgccgtcgc catgaggcag ggcgacagcc tgcaccaaac
     2701 cccgcgcgaa ctgcacgcgt ggctgggcaa gtcccttcag gacggcagcg tccgcatctt
     2761 gtccgccccg atgcataatg tgtcgtcaac ggaaatccgc cgcaaccttg ccggccaagg
     2821 cgtttcagac ggcatcccgc ctgccgccgc acgctacatc cgcgaacacg gtttgtatga
     2881 aaaataaagt caaatcagta agaaacggct ataatgccgt ctgaaaacat cccgttagga
     2941 acataatgaa cgaacaagaa ctgcaagacc tgcaaaaaat ggtcggggtc gccgtcaacg
     3001 ccctcgaaga catcaaagcc aaagacattt ccgttctcga aacgcaagac aaaacttcgc
     3061 tgtttgccag aatgattatc gccagcggcg acagtacgcg ccaagtcaaa gcactggcca
     3121 acaacgttgc cgtcgatttg aaagaagccg gttttgaaat cctcagtacc gaaggagaca
     3181 gcggcgaatg gacgctcgtt gatgcaggag acctcgtcgt ccacgttatg ctccccgccg
     3241 tgcgcgactt ctatgatatc gacaccctct ggggcggcga gaaaccgagt ttccacgccg
     3301 gaatgcagaa gccgtggcac gcggcagact gattcccgat gccgtctgaa tgttcagacg
     3361 gcattttcct gtaagggaga aagcattgaa catcaccgtt ttggcagtcg gcaccaaaat
     3421 gccgcgctgg gttgatgagg ccgtcgccga atacgccaaa cgcttcggac gcgacgtcgc
     3481 ctacgcactc aaagaaatca aacccgaaaa acgcggcgcg ggcgtgaatg ccgcccaagg
     3541 tatggcggcg gaagaaaaac gcatccttga agccattccg caaggcgcgt tcctcgtcgt
     3601 tcttgacgaa cgcggcaaag caccgacctc cgtcgagctg gcggaacacc tcaaaagttg
     3661 gcagcaaaac ggcgaacacg tctgcttcgt catcggcggc gcggacggca tgaccgaccg
     3721 cctcaaacaa caagcccgca tgatgatgcg cctgtccagc ctcaccctgc cgcacggcat
     3781 ggtgcgcgtc tttctgaccg agcagctcta ccgcgccgtt tccatcctgc acaaccatcc
     3841 ctatcatcgg gaataagaag gcttttgtcc gtatccctaa tcggttaaaa tcaccccgtt
     3901 atttctgaac acagcaaagg aatccgctat ggcacgcatg gtattctgcg tcaagctcaa
     3961 caaagaagcc gaaggcatga aatttccgcc gcttcccaac gaattgggca agcgcatttt
     4021 tgaaaacgta tcgcaagaag cgtgggcggc gtggacgcgc caccaaacga tgctgattaa
     4081 cgaaaaccgt ttaagcctcg ccgatccgcg cgcgcgcgaa tacctagctc agcagatgga
     4141 gcagtatttc ttcggcgacg gcgcggatgc cgttcaggga tacgttccgc aataacggtt
     4201 ttccgttttg aacacaggct gtccgaaact gcttcagacg gcctttaaaa tacgccggac
     4261 aactttatct tcacgcaacc ggacaaattt tgacattggg caatgttaaa atcccccatt
     4321 attcccaacc gcgctttcag gagcagatga tatgcaacac gacgtttacg actacaccgc
     4381 gcatacggtt tctaaaaaca ccgtcctgca gaaaacctac cgcctgctcg gattttcatt
     4441 cattccggca gccgcaggcg cggcacttgc cgccaatgcc ggtttcaatt tttacgccgc
     4501 cttcggttcg cgctggatag ggtttgccgt cgtgttggcg tttttctacg gtatgatcca
     4561 cttcatcgag aaaaaccgtt acagcaatac cggcgttacc ctgctgatgg tattcacatt
     4621 cggtatgggc gtattgatcg gccccgtgct gcaatacgcg ctccatattg ccgacggtgc
     4681 gaaaatcgtc ggcattgccg ccgcgatgac cgccgccgtc tttttaacga tgtccgcctt
     4741 ggcgcgccga acccggctcg atatgaacgc gctcggacgc ttcctgaccg taggtgcggt
     4801 cattctgatg gtcgccgtgg ttgccaatct gtttttgggt attcccgcac tcgccctgac
     4861 catttccgcc ggttttgtct tgttcagttc cttaatgatt atgtggcagg tacgcaccgt
     4921 catcgacggc ggcgaagaca gccacatcag cgcggcactg acactgttta tctcgcttta
     4981 caacatcttc agcagcctgc tgaacatcct gttgtcttta aacggcgagg attgatgccg
     5041 gtaaaacgcc gtccgactat gccgtctgaa aatgcttcag acggcatttt actttgggca
     5101 tacaatacgg atgcacacgc accaacacac agccgactat gaagccggga ggaatcaaat
     5161 gttaccgaat acaccgcgcc gcgccgttta tgccggcagt ttcgatccgc ccacattggg
     5221 gcatctgtgg atgatacggc aggcgcaatc tatgtttgac gaactcatcg tcgccatcgg
     5281 cattaacccc gacaaacgca gcacctatac cgtcgctgaa aggcaggata tgttgtgcga
     5341 tattactaaa atgtttccca acgtcagaac cgatgtattt gaaaaccgat ttctggtgca
     5401 ttacgcccgt gaggtagatg caggattcat cgtgcgcggc atccgttctg cttcggatta
     5461 cgaatacgaa cgttccatgc gccatatcaa cagcgacctc gcccccgaaa tatccaccgt
     5521 attcctcatg ccgccgcgcg aaatcgccga agtgtcgtcc actatggtca aaggactggt
     5581 cgggccggaa ggctggacgg aaacgatcca ccgctacgtg ccgcaagctg tgtacgaaaa
     5641 aatccttgcc gaacatcaac acgaaaattg aacccttcta tttttgcaag gcatatatgg
     5701 atatgttgga aattttgttc cgccatcaag acttcgtcgc catcaacaaa ccgggcggca
     5761 tatccgtcca ccaagacagc ggcgagaccg gactggcccg aacactcgcc atacagttgg
     5821 gcgtagagcg tgtgtggctg ctgcaccgat tggacaaaca gaccagcggc attttactgt
     5881 ttgccctcaa cagagaaagc gcgtccgccc ttagcggaca gtttgccggc aaaagcatca
     5941 aaaaaaccta tttggcacta tccgaccgca aaccgtccaa aaagcagggt tggattaaag
     6001 gcggaatgga aaaatccaga cgcggaatgt ggaagttgac gcgcaataca gaaaatatcg
     6061 ccgttacccg attccacagc atcagtatcg ccgaaaaact gcggctgttc atcctggaac
     6121 cgcatacggg caaaacgcac caattgagag tggcgatgaa aagtttgggc agtccgattc
     6181 tgggtgacag cttatacggc ggaacagaat ccgaaaccat gtttctgtat gcatggaaaa
     6241 tacaatttgc ctaccaaaac cgacaaatcg aaattgttgc acccttaaaa aacgaatggc
     6301 agactgaaaa tatctctcat gcgctggaag aattttatat ggcaaaagca ggttaataaa
     6361 tataaattaa tttataaaca atatgataaa tatttttata aaaatttttc aagaaaacgg
     6421 ttgactgcaa atctcttcaa gcgtatagtt cggttcttcg ctgctgcaga agcggcggac
     6481 gaactgaaaa gtatagcaca gaatgttggg gatatcgaga gatatcttga caggcggaag
     6541 agatacttta taattcgcaa
//
LOCUS       DAWFAN010000108         6469 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWFAN010000108 DAWFAN010000000
VERSION     DAWFAN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.108"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(141..1529)
                     /locus_tag="WGO65_001496"
     CDS             complement(141..1529)
                     /locus_tag="WGO65_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0327779.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLIDHILRDADIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            1857..2804
                     /locus_tag="WGO65_001497"
     CDS             1857..2804
                     /locus_tag="WGO65_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0327780.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="WGO65_001498"
     CDS             complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="WGO65_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ0327781.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            3487..3960
                     /locus_tag="WGO65_001499"
     CDS             3487..3960
                     /locus_tag="WGO65_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0327782.1"
                     /translation="MTDFSVWETAPFGATVDYILQRYNNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLAS"
     gene            complement(4030..4851)
                     /gene="nadE"
                     /locus_tag="WGO65_001500"
     CDS             complement(4030..4851)
                     /gene="nadE"
                     /locus_tag="WGO65_001500"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ0327783.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTADLTDTF
                     QTFEQTVGIHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            5048..6403
                     /locus_tag="WGO65_001501"
     CDS             5048..6403
                     /locus_tag="WGO65_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ0327784.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYETRGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPARPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTAVQGAGSEFQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASISKLAQTLSYSMTQNVRT
                     HTARFERQTQALQHYRPDISVCKKNIDRFQTALPAAFSQLLARRRQSLSAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
BASE COUNT         1470 a         1708 c         1743 g         1548 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatattgc agattaatcc tttgggtatc aaagccgggc
       61 ggtgttgaaa cattgcaaaa atgccgtctg aaactcgaaa tcggtttcag acggcattgg
      121 tttatgaggc aggttggggc ttagcggtct ttgtaacggc gttcgctttt gcctgcaaaa
      181 gaatcgccgg ctttacgttt gcctttgaag ccttcgccgg tttttttgaa accgtctttc
      241 ttaaagcctt ctttcttgaa accttcgccg cgcgttttgc cgccgaagcc ttctttgccc
      301 ggtttatgat cgccgcgcca accgccggat ttacgatcgc cccagccgcc tttgcctttc
      361 ggcttgccgc ctgcggattt gcgtttgcgg gtcggttcca tgccctcgat ggtcagttcg
      421 ggcagttttc ggttaatgta tttttcgatt ttgtggactt tgacgtattc gttcacttcg
      481 gcaaacgtaa tcgcaatacc cgtgcggcct gcgcggccgg tgcgcccgat gcggtggacg
      541 tagtcttccg cctgtttcgg caggtcgtag ttgataacgt gggtaatggt cggtacgtcg
      601 ataccgcgtg cggcgacgtc ggtggcaacc aaaattttgc agcggccttt acgcaaatcc
      661 atcagtgtgc ggttgcgcca gccttgcggc atatcgccgt gcaggcagtt ggcggcgaaa
      721 cctttttcgt acagttcatc cgcaatgact tcggtcatgg ctttggtgga cgtgaaaatc
      781 acgcattgat cgatgtcggc atcgcgcagg atgtggtcga tcaggcggtt tttgtgacgc
      841 atatcgtcgc agtacagcag ttgttcttcg attttgcctt ggtcgtccac gcgttcgact
      901 tcgatgattt cagggtcttt ggtcagtttg cgtgccagtt tgccgaccgc gccgtcccaa
      961 gtggcggaga acaataaagt ctgacggtcg ctcggcgttg cttccacgat ggtttcgatg
     1021 tcgtcgataa agcccatatc caacatacgg tcggcttcgt ccagaatcag cacttccaaa
     1081 cgttcaaaat cgactttgcc gctttgcatc aagtccatca ggcggcccgg cgtggcgaca
     1141 atcagatcga ccggtttgct cagggcgcgg gtttggtagc cgaacgatgc gccgccgacg
     1201 atgctgacgg tacggaacca acgcatattt ttggcatacg ccagcgcgtt tttctcgact
     1261 tgagccgcca attcgcgggt cggggtcaac accaaagcac gcgggccttt gcccggtttt
     1321 tcgctgcgtt tggtcagtcg ctgcaaagtc ggtaacagaa aggcggcggt tttgccggag
     1381 ccggtttgcg ccgaagccat gatgtcgcgg ccttccaaag caaacggaat cgcctgcgcc
     1441 tgaatcggcg tcgggctttc gtaaccctcg ctgctgacgg cggacaaaat gtttttatca
     1501 aggttcaaat cggcaaattt aatagacatg gctatccttg gaagacaaca acgcccgcgt
     1561 ccgaaaggtt cagacggcct gaagcaggga agggtaacga tacggggaat gtgaagttac
     1621 agggttgtcg caagcggctt gcttgtggtt tacatcgacg gcaacctgca cagatacgct
     1681 ttgcaaaatc aaagcaacag aagaaacgcg cccgtaaggg cggtttaggt tggaaggcga
     1741 tggcttcgta tgacaagata agcggtggat ggtatagggt ttgcccgaaa cggtcaagca
     1801 atcgggcgtt aattacagta aaattgccgt tttccacttt attccgcctg aaaccaatga
     1861 ccgacatcac accgtttgcc aaccgcttgg gcaaaaacat caaacacctt atgaaatggg
     1921 cgaaacgcaa cggcatcgaa gcctggcgca tttacgaccg cgacatcccc caattcccct
     1981 ttgccgccga tgtttacggc gatcgaatcc accttcagga atacgatacc ggctggctga
     2041 tgcggcccga agaatacgaa gcgtggcttg ccgaagtctt ggaagccgtc gccttcgtta
     2101 ccggttttgc gcccgaacaa atccgcctca aacgccgcga acgccaaaaa ggtttgcagc
     2161 aatacgaaaa aaccggcaag gcgggcgacg atttcgtcat caccgaaaac ggccgcaagt
     2221 tttgggtcaa ccttgataag tatttggata cggggctttt tttggatcat cgaaacacgc
     2281 gcaaaaaagt cggcgaaacg gcggcgggca aacgtttttt aaacctgttt tcctacaccg
     2341 gcagcttcac tgtctatgcc gccaccggcg gcgcggcatc cagcgaaact gtcgatttgt
     2401 ctaacactta tctcgattgg gcgagacgca attttgaatt gaacggcatc gatacggaac
     2461 ggcataaaat cgtccgcgcc gacgtgttcc aatatcttca gacggcatat ggggaaggca
     2521 ggcggttcga cctcattgtg atggacccgc ccagtttttc caacagcaag aaaatgtccg
     2581 acatcctcga catccagcgc gaccacaaaa agctgattga cggcgcggtg aagctgctcg
     2641 cttcagacgg cattttgtac ttttccaaca acctgcgcag cttcgtctta gacgacttgg
     2701 tatcggaaca atacgccgtc aaagacattt ccaaacaatc cgttcccgaa gatttccgca
     2761 acaaaaaaat ccataggtgc tgggaaatcc gccacaaatc ttaagggtgc gaaaaagccg
     2821 ccggaatctt ccggcggctt tttcgattgc gctttatcag gcgatagagg cttcgacaaa
     2881 ggcggtcagc tgaccttttg ccaatgcgcc gactttggtg gcgacgactt cgccgttttt
     2941 gaacaccatc agggtcggaa tgccgcgcac gccgaaacgg gacggggtgg cttcgttgtc
     3001 gtcgatgttg attttgacca ctttcagacg gccttcaaat tcggcggcaa tgtcgtccaa
     3061 aatcggggca atcattttgc aggggccgca ccacggagcc caaaagtcca gcaggacggg
     3121 gatatctgca tttaaaacgt ctttttcaaa agcggcatcg ctggtgtgta caatcaattc
     3181 gctgctcatt atttttcctt ttcggttggg attggcacga tagcgtgcag gatagggctt
     3241 gggcataaaa atttcaagtg ggcgggcggg ataatagttt ttctgtaccg ccccaatcgc
     3301 ctttgcaaat tctaaaaaac ggttcggaca gtattttatt tttgaaaatt acttttatta
     3361 tcaaagtgta aggaatgttt ttgaagctgt acagaaacgc gcgggcggtg tatgctccgt
     3421 ttaaatagat tcttaaagta tgaatgttca aaaccaaacc cgaaacccta taaaaggaac
     3481 actgcaatga ctgatttttc cgtttgggaa acagccccct ttggcgcaac cgtcgattat
     3541 atcctgcaac gctacaacaa tgtccaccgc gcacaatttg aagaattggt gccgctggcg
     3601 cagaaagtgg cccaagtcca tgccgatacc tttccggcgg aaattgccgg gctgcttgcc
     3661 tatatgcaga acgaactgct gatgcatatg atgaaagagg aaaggatgct gtttccgatg
     3721 attaatcagg gtgtcggacg cggtgcggca atgcccatca gcgtgatgat gcacgaacac
     3781 gaagaacacg accgcgccat cgcacggctc aaagagctga ccgacaattt ccaaccgccc
     3841 gaaggggctt gcggaagctg gacgcggctt tacgcgctgg cgaaagaaat ggcggacgat
     3901 ttgaacgacc acattcattt ggaaaacgat attctgtttg cccgcgtact tgcttcttaa
     3961 ggaacggaag ccggatatgt caatgccgtc tgaaacacgg ttttccgctt cagacggcat
     4021 tttcatgttt cagcccaaca attcgggcgg aatgcggcac acgggaatcg ggttgatttt
     4081 gtgctgcatg gcgcggtgaa gtcgcgtata gatttctaga acttcgcgct gccgcccttc
     4141 aaaatcttcg ggtttgcgcg tgccgtacac gcccattgcc cactccagtt cgggatagct
     4201 tgcgcccatc tgttcttcgt cggtgcgttc cgtatcccac aggccgtcgg tcggcggggc
     4261 tttttgaatc gcctcgtcca cgcccaatgc ttcggcaagc cggtaaacct gcgttttggt
     4321 caggtcggca atcgggctga tgtccacgcc gccgtcgccg tatttggtaa aaaatcccac
     4381 gccgaaatct tcaattttat tacctgtccc cgtaaccagc agtccgtgta tctgcccgta
     4441 gtagtacagg gtcagcatac gcaggcggct tctggcgttg gcgagggaaa gcggctgatt
     4501 gtcaaatgcc gtctgatgaa tgccgacggt ttgttcaaag gtctggaagg tgtcggtcaa
     4561 atcggccgtt tgcgcgctta cattggcata ttgccgttgc agattgcgga tgtgcagcct
     4621 tgcccgctca agctggtcgg ggtgttggcg tatcggcata tccagaagca gcgtggtgcg
     4681 gccggtgcgg gcggcgagtg cggagacgac ggcggaatcg atgccgccgg aaacgcctac
     4741 gacgaaccct tttgcatttg cccgggcggc gtattcgtcc aaccatcggg cgatatgtgt
     4801 gataactgcc tgcgtatcca tttttcctcc tgtcgaaaat atccgcatca tagcctgccg
     4861 gcggtgcaat ttgaatagat tgcaggcagg ttgatgaata tttttcccat tccccgctta
     4921 aaaaagtcat acgggaaaag gcgggcggca agatggcgcg tgttataata ttgttgacaa
     4981 tttaggcggc ccgatgccgc cttgggcttc aaaacatatt catatttttt attatttgat
     5041 tgattcgatg tctgattttt ttcattctga cgtgttgtcc gtttccgagt tgaacgcatt
     5101 tgccaaaagt attttggaaa accaccttgc cggtttgtgg attgccggcg aagtgtccaa
     5161 cctgacccgc gccgccagcg gacattatta cttctcgctt aaagacagcc gcgcgcaggt
     5221 gcgttgcgcg atgttcaaag gtgcggcggc gcgtttggcg aagcctttga aagaaggcga
     5281 ccatatcgaa gtatcaggga aaatcagtat ttatgaaacg cggggcgaat ttcagattac
     5341 cgtgaacgaa gtgcggctca agggcttggg gcagctttac gaagcctatg agcggttgaa
     5401 ggcgcagttg caggcggaag gcgcgtttgc ggcggaacgc aaaaaacctc tgcctgcccg
     5461 accgcaatgt atcggcatcg taaccagttt ggcggcggcg gcattgcgcg atgttgtaac
     5521 caccttgaaa cgccgcgcgc ccgaaattcc cgtcatcgtt tatccgactg ccgttcaggg
     5581 cgcgggcagc gagtttcaga ttgcccaagc gattaaaacc gcatcgcaac gcgccgaatg
     5641 cgacgtgttg attgtctgtc gcggcggcgg cagcattgaa gatttgtggg cgtttaacga
     5701 agaaccggtc gtacgcgcca ttgaagcctg cacgattccg gtcgtcagcg gcgtaggcca
     5761 cgaaaccgat ttcacgcttg ccgattttgt tgccgacgta cgcgcaccga caccgaccgg
     5821 cgcggcggag ctggtcagtc ccaaccgcca agaatcgtta catcgtctag cccaagcgca
     5881 aggccgtctg aaaactgttt tggagcaacg ctatttcgat gccagccaaa aactcgactg
     5941 gctcgcacgg caaatccgtc atccgcgcca aaaacttgac gagcaacgcg cttcaatcag
     6001 caaactggcg caaacgctgt cttactcgat gacgcaaaac gtccgtaccc acaccgcccg
     6061 ttttgaacgc caaacccaag ccctgcaaca ttaccgtcct gatatttccg tttgcaaaaa
     6121 aaacatcgac cgctttcaga cggcattgcc cgccgccttc agccaattgc tcgcccgccg
     6181 ccgccaaagc ctgagcgccc aagcagcatt gctcgaagcc gtttcgccgc agcacattct
     6241 ggagcgcggc ttttccgtcg tcaaaaacac acgcggacaa gtcatccgca acgccgatgt
     6301 tttgaagcag gggcagaagc tgcacattat cttcgccgac ggcgaaaccg acgtgcgcgt
     6361 aagcaaagaa caagggcagc aggatttgtt cgattgcatt taaacccaat cggaaaaacc
     6421 gctccccctc cgccgtcatt cccgcgaaag cgggaatcca gtcccttga
//
LOCUS       DAWFAN010000109         6343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWFAN010000109 DAWFAN010000000
VERSION     DAWFAN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.109"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(57..1511)
                     /locus_tag="WGO65_001502"
     CDS             complement(57..1511)
                     /locus_tag="WGO65_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ0327785.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(1508..2209)
                     /locus_tag="WGO65_001503"
     CDS             complement(1508..2209)
                     /locus_tag="WGO65_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ0327786.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(2206..2985)
                     /locus_tag="WGO65_001504"
     CDS             complement(2206..2985)
                     /locus_tag="WGO65_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ0327787.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            3269..4675
                     /locus_tag="WGO65_001505"
     CDS             3269..4675
                     /locus_tag="WGO65_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0327788.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLNMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLVGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            4787..6313
                     /locus_tag="WGO65_001506"
     CDS             4787..6313
                     /locus_tag="WGO65_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0327789.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPKYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
BASE COUNT         1368 a         1830 c         1599 g         1546 t
ORIGIN      
        1 aaatgccgtc tgaagccctg tttcgggttt cagacggcat ttttgcgaac aactttttat
       61 gcctgttctt tttggcgcat tttttctgcc atcagttcgt gcagggtttt cttcgccggt
      121 ttcatcggca cgcggttttg cgtccaaccc aactgtttgc gcggggtcag gttgcggaac
      181 ttggtggctg cccaaccgaa ggcgcggtag gttttgctgc cgctgaaaat accgttgaat
      241 gtgcgccacg ccatttgttc gccgaaggta tgcgatgcgc cttgccctcg gatggggtgc
      301 ggcacggttt cggtcggcga acgttgcgct tcaacgcgca aacgctgcat ttgttcggta
      361 atcgggatgc gtaccggaca aacttccacg cacgcgccgc acatcgtgca ggcggttggc
      421 aagtcgcggg tggcatccag acccaacagg tgaggggaaa taatctcgcc aatcgggccg
      481 ggataggttg tgccgtatgc cgcgccgccg atacgggtat aaaccgggca atggttcata
      541 cacgcgccgc aacggataca ttgcaaggtg cggcgcattt ggtcttcggc ataggcttgg
      601 ctgcgaccgt tgtcaagcag gaccaagtgc atttcttgag gaccgtccag ctcttcgcta
      661 cggcgcgggc cggtaatcat attgaaataa gtggtaatgt tctgaccaat ggcagaacgc
      721 ggcagcaggc tgtacaaggg tgggatgtcg gacaatttcg ccaccacttt ttcaatgccg
      781 gtaatagcga tatgcacggg cggtacggtg gtactcaagc gaccgttgcc ttcgttttcc
      841 accagacaca gcgtacccgt ttcggcaaca gcaaagttta cgccactcaa accgacatcg
      901 gcagtgctgt aaatatcgcg cagggctttg cgggcgaagc cggtcagttg gtccacgtcg
      961 tccgtaagcg gagttccaag gttttggtgg aacagttcac tcacctgttc tttggttttg
     1021 tgaatggcgg gcatcacgat atgggtcggt ttttcgcctg ccatttggac gatgaactcg
     1081 cccaagtcgc tttctaccgc tttaatgcct tttgcttcaa gataatggtt cagctcgatt
     1141 tcctcgctga ccatcgattt gcctttgacc atcagcttgc cgtttttggc tgtgatgatg
     1201 tcgtggataa tttggcaggc ttcggtcggg gtttctgccc agtgcacttt cacgcccaac
     1261 ttagtcaggt tttcttccag ctgctccagc agggctggca atttagacaa tgagcgctga
     1321 cggacgtgtt cgcacaaatc gcgcaggctt tgcagctctt cttcgtcggt caaaacggct
     1381 ttgcgtttgg tcatcagcat atccatcgcg gtacgcaggc ttttgcgcaa aggcttgtct
     1441 tgaagggaaa ttgcggcgtt ttgcttgaaa gtttccggct tcatgtgaaa tttgatggtt
     1501 tgcgtagtca tgcgttttcc tccaaatcgg caggggaaat gtggtcgggc aggatggcga
     1561 ggatgaccaa atcgcgcggg ccgtgcgcgc cgtaagcaag cgtcagttgg atgtctgcgg
     1621 ttttggacgg gccggaaatc aggaatacat tggtcggcat accgttttcc accagttttt
     1681 cgccttcgac ggcattatga aactcgttgt acatcttgga cgtatcgaac aggcagaaat
     1741 gcacgggcgg aacgagactc aaagtacgcg gttcttcggg gctggaaaac agcatcagcg
     1801 tgccggtgcg ggcgatgccg cattgcgcgc cgctgaagcc cgcatcgatg ttcgtgaaaa
     1861 actcggtttt ccaagtatcg atttcgcgct cgaaggcaat cggttcgata ttgctgtccg
     1921 ccaatgcggc acgggcaatt tgtccgtgtt cggtcgccaa gggcagcagg atgtttttca
     1981 aacccttgcc ttctgccgct tcgcggaaaa cctgcatcca attgcttttc gtcacccaat
     2041 aaatttcggt tttgaccgca cgcatagcgg cagcccaatg tttcagacgc tcaacttcgc
     2101 tgccccaaga aacacccatt tcacggtaat aatcaaaaac cgcaggttct tccatcggca
     2161 atgcgtcggc ttttttcagt tttgccaaaa tattttcgcg cgcgctcatg ctttgcctcc
     2221 ggtacgttcc aacaagaagg atgcgatatg tttcggacgc ggcatattcg gttcgtcctt
     2281 agcaattttg ccgccgatgt tcatcataca gccgcagtcc gcgctgatga tttcggttgc
     2341 gccggtttct ttcagcgcgg cgactttgtc ggttaccatc gcgccggaaa tatcggcttg
     2401 tttgacggag aatgtgccgc cgaagccgca acattcgctt tcgtggtcgt ggacgatgcg
     2461 ttcgacgttt tccataccgt caatcagttg ccagcccgag agatggacat tcatttcgcg
     2521 gcgggcggcg caggaagtgt gaacggcgac tttgagcggt tcgcccttgt cttcgggttt
     2581 gaaaccgatg gcaagcagga aatgggtaaa ctcgatgatg cggccggcgc aatccacagc
     2641 cctttcctcg tactcgctgc ctttaaacag cgtcggccaa tggtgcttca tcataccgcc
     2701 gcacgaaccg gacggtacga caatcggcca ctcctcgggg aaaaggtcga gttgcgctct
     2761 ggcgacatca aaggcttcgg tcggatggcc ggatgaatag gcgggctgac cgcagcagct
     2821 ttgcgccatc gggaaatgga cgcgtatgcc ctgttgctcg attagggtaa tggcatccat
     2881 gcctgcttcg ggcataaaaa gatcaaggac gcaagtgccg aaaaaataga catcggtcgg
     2941 attgctgtcg tatcggatga tttttggggg aatggttgcg ctcatgtttt ttgataacgg
     3001 gaaacccgtt tttttctgta gaaaggtaag cgtttacttt aagtaattga ctgttgcggg
     3061 tcaagtctaa ttttaaaaaa taatccggtt tttcttacaa actaccccat aacgcttact
     3121 gtaccttaat ctgatagttt tcgataataa ttatcattac aatgcaatgc cggttcgttt
     3181 gcttgtgaac attcaagatg ccgactctga cggcattcag acagcatctg aaaacaataa
     3241 cggcacaagg atggggtaca atcgctctat ggaaaaaaac accctatccg cccgcgcacc
     3301 atccccttgg ctgccgcttc tgctggcaat tgccattttt atgcagatgt tggatgcgac
     3361 cattttaaat accgcactgc ctgaaattgc cgccgacctg aatgagtcgc cattgaacat
     3421 gcagctggcg gttatttcct acacgctgac ggttgccctg ctgattcctt tgagcggtta
     3481 tttggcggac aggttcggaa cgaaaaaagt ctttttcggt tcgattgccg tttttatgct
     3541 cggatcggca ttgtgcgccg catcgggttc gctgtttgaa ttgacgcttt cccgtgtcgt
     3601 tcagggcatc ggcggttcga tgctggttcc gataccgcgt ctgaccatct tgcgtgtgta
     3661 cgacaagtcc aagctgctca acgccatcaa ttatgcggtt atgcccgcat taatcggacc
     3721 ggttttaggg cctttggcgg gcggttatct ggtcgaatac gcttcgtggc actggatttt
     3781 cctgctcaac ctgcccatcg gtcttctggg tttcatattg ggacgcaaca tcatgcccga
     3841 tattaaaggc agcaatatct ctttagactt caaaggctat ctgatttttt ctgccgccgc
     3901 gtgcctcttg ttactttcgg cagaaagcct gtcgcacgcg ctgcctccgt attttgcact
     3961 gttgccgctg tgcggcggac tgctgtttgc acgccgttat ttccgacata tgaaaaccgc
     4021 gtccaaaccg atttattccg ccgacctgtt tctgatacgc actttccgtc taggactggt
     4081 gggcaatctg ttcagccgtc tcggcatcag ctcgattcct tttctgatgc ccctgatgtt
     4141 tcaagtcgct ttcggcttcg gcgcaagcct gtcgggttgg ctggtcgccc ccgtcgccct
     4201 gtcttcgctg ctggtcaaac cgctgattgc accgctcatg aaacgtttcg gctaccgcac
     4261 ggtactgctt tggaacacca agctgcttgc cgccttcatc atgctgctcg ccctacctga
     4321 tggaaactcg ccgctgtgga tttggatttt cctctcgctg gcgatcggcg cgtgcaactc
     4381 cctacagttt tctgccatga acacactgac cctcgccgat ttgcgcccgc aacagaccgg
     4441 cagcggcaac agcctgatgg cggtcaacca acagcttgcc atcagcatgg gcattgttgt
     4501 cggcgcatta atccttaaaa actggacatt tctgataccg gcttcttcag gtctgcattc
     4561 cgccttccgt atgaccctgc tcagtatcgg cggcattacc cttgcctcgt cgttggtttt
     4621 caagcgtctg cacgtttcag acggtgcaaa cctgacacgg aacacaccgt cctaaagcgg
     4681 tccgcacgca aaacttttac ccgtttcaac gtttggatta tgattccgca cttccatgcg
     4741 caccaacccc aaaacacagg caatgccgtc tgaaaccata tccctgatga aaacacgcag
     4801 cctaatttcc cttttatgcc tccttctctg ttcatgttct tcatggttgc ccccactgga
     4861 agaacggacg gaaagccgtc atttcaatac ttccaaaccc gtccgcctgg acaacatcct
     4921 gcaaatccgg cacacccctc ataccaacgg gctatccgat atctatctgt tgaacgaccc
     4981 ccacgaagcc tttgccgccc gcgccgccct tatcgaatct gccgaacaca gcctcgattt
     5041 gcaatactac atctggcgca acgacatttc cggccgactg ctgttcaacc tcgtgtacct
     5101 tgccgcagaa cgcggtgtgc gcgtacgcct gctgttggac gacaacaaca cgcgcggatt
     5161 ggacgacctc ctgctcgccc tcgacagcca tcccaatatc gaagtgcgcc tgttcaaccc
     5221 cttcgtctta cgaaaatggc gcgcactcgg ctacctgacc gacttccccc gcctcaaccg
     5281 ccgcatgcac aacaaatcct ttaccgccga caaccgcgcc accatactcg gcggacgcaa
     5341 tatcggcgac gaatacttca aagtcggtga ggacaccgtt ttcgccgacc tggacatcct
     5401 cgccaccggc agcgtcgtcg gcgaagtatc gcacgacttc gaccgctact gggcaagcca
     5461 ttccgcccac aacgccacgc gcatcatccg cagcggcaac atcggcaagg gtcttcaagc
     5521 actcggatac aacgacgaaa cgtccagaca cgcgctcctg cgctaccgcg aaaccgtcga
     5581 acagtcgccc ctctaccaaa aaatacagac aggacgcatc gactggcaga gcgtccaaac
     5641 ccgcctcatc agcgacgacc ctgcaaaagg actcgaccgc gaccgccgca aaccgccgat
     5701 tgccgggcgg ctgcaagacg cgctcaaaca gcccgaaaaa agcgtctatc tggtttcacc
     5761 ctatttcgtc cccacaaaat ccggcacaga cgcactggca aaactggtgc aggacggcat
     5821 agacgttacc gtcctgacca actcgctaca ggcgaccgac gttgccgccg tccattccgg
     5881 ctatgtcaaa taccgaaaac cgctgctcaa agccggcatc aaactctacg agctgcaacc
     5941 caaccatgcc gtccctgcca caaaagacaa aggcctgacc ggcagctccg taaccagcct
     6001 gcatgccaaa accttcattg tggacggcaa acgcatcttc atcggctcat tcaacctcga
     6061 cccccgttcc gcacggctca atactgaaat gggcgttgtt atcgaaagcc ccaaaatcgc
     6121 agaacagatg gagcgcaccc ttgccgatac cacacccaaa tacgcctacc gcgttaccct
     6181 cgacaggcac aaccgcctgc aatggcacga tcccgccacc cgaaaaacct acccgaacga
     6241 acccgaagcc aaactttgga aacgcatcgc cgcaaaaatc ctatccctgc tgcccataga
     6301 aggtttatta tagaaatgta gcggattaac aaaaaccagt acg
//
LOCUS       DAWFAN010000110         6270 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWFAN010000110 DAWFAN010000000
VERSION     DAWFAN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.110"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(64..624)
                     /locus_tag="WGO65_001507"
     CDS             complement(64..624)
                     /locus_tag="WGO65_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ0327790.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPA
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1041..2081)
                     /gene="mutY"
                     /locus_tag="WGO65_001508"
     CDS             complement(1041..2081)
                     /gene="mutY"
                     /locus_tag="WGO65_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ0327791.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2244..2378
                     /locus_tag="WGO65_001509"
     CDS             2244..2378
                     /locus_tag="WGO65_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine acetyltransferase"
                     /protein_id="HEZ0327792.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     "
     gene            2365..3189
                     /locus_tag="WGO65_001510"
     CDS             2365..3189
                     /locus_tag="WGO65_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEZ0327793.1"
                     /translation="MWVFEKAKKTEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVII
                     SCVSKCCTCDNCKIQLYSHCCNGGWILGYMIDGTQAEYVRTPYADNSLVPLPDNVNEE
                     VALLLSDALPTAQLYSPAAIIVCDMDENRLKLAKELGATHTINPASGDVSKQVAAIVG
                     EDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKLEKLWIKKLAITTGL
                     VNANTTEMLMKAISSSSVDYTKMLTHRFKFSELEKAYDVFKHAAENQAMKVVLEAD"
     gene            complement(3213..3431)
                     /locus_tag="WGO65_001511"
     CDS             complement(3213..3431)
                     /locus_tag="WGO65_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327794.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(3446..4084)
                     /locus_tag="WGO65_001512"
     CDS             complement(3446..4084)
                     /locus_tag="WGO65_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ0327795.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     RNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4265..6100)
                     /gene="edd"
                     /locus_tag="WGO65_001513"
     CDS             complement(4265..6100)
                     /gene="edd"
                     /locus_tag="WGO65_001513"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ0327796.1"
                     /translation="MNPTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NNTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDPIRFDSVTGELNVLINETE
                     WNAREVEQIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
BASE COUNT         1387 a         1498 c         1771 g         1614 t
ORIGIN      
        1 gctaaatatc tagcaaaaaa gtgcggtcaa aattcaccgc acttttcgtt tcgaacaatc
       61 gaattattta atcacgccac atgccatacg tgggccgcca ccgccaagtg gagctggatg
      121 atcggagtga ttatcaccac ccgtgtggat cataatagag tgaccgcgaa catcatctaa
      181 atgtttaaga cgtggtgcta aaacaggatt tgttgctgtg ccatcatgca atacagttaa
      241 tgcaggtaaa tcacctaagt gtgcatcatc ttgccatggg taaccatgtt gttttgcacc
      301 tttaggatcc cagtgaccgc ctgcgcctaa accagctgtc aatttacctt ctttttcttt
      361 tggctcacag cttgggtttt catggatgtg gaaaccatgt aagccttcgc ttaatccttg
      421 taaatcaggg gtaaacacaa gaccatagtt agattcagta atagtcactg tacccacatc
      481 tttgttaccg tttgctggat caagttgttg cactttcact tcaatagaag cacctttagg
      541 tatcgtatta tgctcgtgtg cttgcgcaac accaactgaa catactgcac taaccgctag
      601 tgctaataag gttttcatat tcattgcaat ctcctttatt gcagtaaaaa ctcgcgacta
      661 ttttagtcaa ttttgtttta aaagccgata aatttttacg aaaattttac attaattgtt
      721 atttgcagaa aattagaacc cgttatcacg tcttggataa atgccaaaac cattgtgatt
      781 cagagaatta aaaagaaaaa tttcggaatc ctcatccttt tctgaaacct gattgcggta
      841 tccatggctt tgtctttgtc atttaagttt gtgttcatgt ttttgacctt ctttgctttt
      901 taaatccttt ttaaattgcc gacttcttcc gttccaaatg tcccatttga acattatccc
      961 accgcctaaa atacgaccca aattcatcgg ttgttcttgc tgcgatacct gcaaacaaca
     1021 gttcaatggg tttattttgt ttattctaac ctattaccgt ttaaataaat ttccaaaggc
     1081 ttgggcaggc cgtaatcttt caaatgcgcc ggctttatcc aaatgccgtc tgaagggctt
     1141 tcagacggca tttgtccttc aaacggcgta atcatcagca gccggtgcgt cagacggtgg
     1201 gtcagggcgg tttgttcgtc catatctgcc atggtcaggg agaatttggc ggcaaagtcg
     1261 gaaagcccgt tcaaactttc aaaacacggc acgcaataca gcccgcccca aatgcctttg
     1321 gcggggcgtt tttccagcaa aatcgcgccg tcccggttgc ggacaatcag ccagtaaagc
     1381 ggcagggtcg gtacttcggc ggcggttttt ttgcgcggca gctcggcggt gcggttttgc
     1441 ttttttgctt cgcagatgtc cgccatcggg cattggtggc acaagggttt cgtccgtttg
     1501 cacacggtcg cgcccaaatc catcaaacct tgtgtatagg caggcatatc ggcgttttca
     1561 gacggcagca ggctttcggc aagtgtccag agcgagtttt caaatttttt gtcctgcgga
     1621 ttgccgtcgc gggcgaacac gcggcagagt acgcgtttga cgttgccgtc caaaatggtt
     1681 tcgcggcggt tgaaggagaa ggcagaaatg gcggcggcgg tgcttctgcc tacgccgcag
     1741 agggtttcca agtctttgcg ctccgacgga aacgtgccgc cgaattgcct gacgacttgt
     1801 tgcgcggctt tgtgcaggtt gcgcgcgcgg ctgtaatagc ccaagcccgc ccacaacgac
     1861 aacacttcgt cttgcggcgc ggcggcaagc gtctgaacgg tcgggaattt ttctaagaag
     1921 cgcggatagt agtccaacac ggtggcgact tgcgtttgct ggagcatgat ttcggaaagc
     1981 cagacgcaat aagggttttt gacctgccaa gggaggtggt ggcgaccgtg ttgtttttgc
     2041 cagcggatga gccgttcgga gaaggggatg ggtgtgttca ttaatatcaa tcggtggttt
     2101 tatttatatt taaaacggta tgttattgta taacattgtg aaaataattc ttattgactt
     2161 attttttgta ggggtatata actcatataa agaaacttta ttgcggtatt gaaattattt
     2221 atcaacaagc aaggagtatc agcatgaaag caatggttta tcacggcgca aacgacatcc
     2281 gttttgaaga aaaaccccgt ccgcagatta tcgatccgac cgatgcggtg gtgaaaatcg
     2341 tcaaaaccac gatttgcggt accgatttgg gtatttgaaa aggcaaaaaa aaccgaagtc
     2401 gccgacggtc gtattctcgg tcatgaaggc atcggtattg tagaagaagt cggcgaggct
     2461 gtaaaaaaca tcaaagtcgg cgataaagtc attatttcat gcgtcagcaa atgctgtact
     2521 tgcgacaact gcaaaatcca actttattcg cattgctgca acggcggctg gattttgggc
     2581 tacatgattg acggcacgca ggcagaatac gtccgcacgc cttatgccga caacagcctc
     2641 gtgccgctgc ccgacaacgt caacgaagaa gtcgccctgt tgttgagcga cgccttgccg
     2701 accgcccaac tgtacagccc cgccgccatc attgtttgcg atatggacga aaaccgtttg
     2761 aaactggcga aagagttggg cgcaacccat accattaacc ctgcttccgg cgatgtaagc
     2821 aagcaagttg ccgctatcgt cggcgaagac ggcgtggact gcgcgatcga agccgtcggt
     2881 atccctgcaa cttggaatat gtgccaagac attgtgaaac ccggcggcca tatcgccgtt
     2941 gtcggcgtac acggtcaatc cgttgatttc aaactggaaa aactgtggat taaaaagctt
     3001 gccatcacta ccggcctggt aaacgccaat accaccgaaa tgctgatgaa ggcaatttcc
     3061 agcagctccg tcgattacac caaaatgttg acccaccgtt tcaaattcag cgaattggaa
     3121 aaagcctacg acgtgttcaa acacgccgcc gaaaatcaag cgatgaaagt ggttttggaa
     3181 gcggattaat cggccttgaa tcagttgttt ttttaagcgg atgccgtctg aatgcgtttc
     3241 agacggcatt ttttcagccg cgtagtatgg gctgtccggc tgctgtcgaa tgaactgaac
     3301 gttattttta ttatgcggaa ggcggatggg cattttaggg gcagtatgtc ggagtggtgc
     3361 tgccgtgtat gccgactgcg ccgccaagcg gcaaaatgcc gtctgagact ggaaagggct
     3421 tcagacggca tttttacgat gcgaatcagg ctttgggttt caatgccgcc gcttctttgg
     3481 cgagacgggt gatggtgtcc cagtctttgt ttttcacggc ttctttcggc gtcagccatg
     3541 agccgccgac gcacaggacg ttgggcagtg ccaagtaatc gggcgcggtg gcgaggctga
     3601 tgccgcctgt cgggcagaag cgaacatcgg cgtaagggcc gtacagggct ttgagcatgg
     3661 ctttgccgcc aacgacttcg gcagggaaga gtttgagggt gtcgatgccg tgttccaaag
     3721 ccagttggat ttcgcccggg gtggcaacac cgggaatcag gggaatgccg ctgttgcggc
     3781 cggctttggc gagggattcg tgcagacccg ggctgatggc gaaaaccgcg cctgcgtctt
     3841 cgacggcttt gagctgttcg ggattggtta ccgtacctgc gccgacgatg gcgttgggca
     3901 cctctttggc aatcaggcgg atggcatcga ggccgacagg ggtgcgcagg gtgatttcga
     3961 gggtagggat gccgccttcg acaagggcgt gggacaaatc gatggcggtg cttaagtcgt
     4021 caatcgccat taccggcaca actgcgccgg cggtcaaaat ttcgcggggg gtcagtttgg
     4081 acatttcggt tctccgggta ggatagggta ttttattaag atgggacagg ttgtaggtcg
     4141 gattcaagaa accgacatct tggacggctg tgtcggattc gagaatccaa cctacgtcta
     4201 tcttaaaacg cttgaataat ttttcagacg gccttcggtc tgaaaaggcc gtctgaaacg
     4261 cgcatcaggc aaattcgccg ccgaagctca tcgcaccggt ttccgcgctg ctggtcatgc
     4321 tgcggaagcc ggcgaagagt tcgcgaccgc agccttgttg gttcgcgccc aagtcgattt
     4381 gttcgacttc gcgggcattc cattcggttt cgttgatcag aacgttgagt tcgccggtaa
     4441 cggagtcgaa gcggatcggg tcgccggtac gaattttggc gatgttgccg cccatcaggg
     4501 cttcgggggt catgtggatg gaggccggaa ctttgccgga cgcgccggac atacggccgt
     4561 cggtcagcag cgccactttg aagccgcggt cttgcaggat gcccaaaggc ggggtcagtt
     4621 tgtgcaactc gggcataccg ttggcacgcg ggccttggta gcggacgacg cagacgaaat
     4681 cgcgttccaa ttcgccgcgc tcaaacgcag ccaacacttc gcgttggtcg ttgaacacga
     4741 tggcaggcgc ttcgataatg cggcagcctt cgcgcacggc ggacacttta atcacgccgc
     4801 gaccgatgtt gcctttcatc aggcgcagac cgccgtctgg ggagaacggg ttgtcggctt
     4861 tgcgtaggat gtcgtcgttg ccgctggttt cgggggcttc tcgccattcg agtttgccgt
     4921 cgatgaggaa aggctctttg gtgtagtggc gcataccgtg tccgacgacg gtatcgacat
     4981 cgtcgtgcaa caggcctgcg tccagcaatt cgcggatgac gaaaggcagt ccgcccgctg
     5041 cggtaaagtg gtttacgtcg gccttgccgt tcggataaac gcggatgagc agcgggatga
     5101 tggaggaaat ttcatcgaag tcgtcccagt tgagaatcac gccggccgca cgcgccatag
     5161 cgacgaggtg catggtgtgg ttggtcgagc cgccggtcgc catcagaccg atcagggcgt
     5221 tgataaagga tttttcggtc aacatttcgc ccaaaggttt gatggtgttg tttttaatgc
     5281 cgcgcgcgag gtgtccggcg gcgtagcggg tcagcgcttc acgcaggtcg gtgtaagggt
     5341 ggacgaaagc ggcggcaggg aggtgcacgc ccatcatttc catcatcatt tggttggagt
     5401 ttgccgtgcc gtagaaagtg caggtgcccg ggctgtggta agaacccatt tcgcttttca
     5461 aaagttcgtt gcgtccgacc ttgccttcgg cgaaaagctg gcgggtgcgg gctttttctt
     5521 tgttgccgat accgctggac atcgggcctg cggggacgaa gatacctgga atatgaccga
     5581 acgaaagcgc gcctatcatc aagcctggaa cgattttgtc gcatacgccc ataaacaggc
     5641 tgccgtcaaa catttgatgc gacagcccga cggcggtact catggcaatc acgtcgcggg
     5701 agaacagcga cagttccata ccggcgtagc cttgcgtgat gccgtcgcac atggcgggtg
     5761 tgccgccggc gacttgggcg gtcgcgccgt ttttctgcgc ttcgtctttg atttggtcag
     5821 ggaagtcttt aaacggctgg tgtgcggaaa ccatgtcgtt gtaggcggtg atgatgccta
     5881 agttggggac ggtttcctga agcatttcga ttttgatgct tttgggcatg gcggcatagc
     5941 cgtgcgccaa attgctgcag ccgagttggt tgcgctccaa gcgtcccatt tgtttggcgc
     6001 tgcggatttt cgccagatat ttttcacgcg tcggacggct gcgttcaata atgcgcccgg
     6061 tgatttcggc gagtttggag tggataggag tggggttcat gttcggtctc ctgtcggaag
     6121 tgggtggtat ggggattttg tttgcgcgtt tctgattttt tgtattgggt gtggggaatt
     6181 tggcgtttca gacgaccttt caaatcttac aaggggtcgt ctgaaaacct cactatagtg
     6241 gattaacttt aaaccagtac ggcgttgcct
//
LOCUS       DAWFAN010000111         6263 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWFAN010000111 DAWFAN010000000
VERSION     DAWFAN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.111"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..131)
                     /locus_tag="WGO65_001514"
     CDS             complement(<1..131)
                     /locus_tag="WGO65_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ0327797.1"
                     /translation="MLKTLTFAALHFTTAFSVTYVLTGSIGVSGAVALVDPLINTVV"
     gene            254..1207
                     /locus_tag="WGO65_001515"
     CDS             254..1207
                     /locus_tag="WGO65_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0327798.1"
                     /translation="MNHKKIVVLDADTLPGRAFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAEAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIQDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPAVREGYVAFENAVRTADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLKARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1281..3338)
                     /gene="metG"
                     /locus_tag="WGO65_001516"
     CDS             complement(1281..3338)
                     /gene="metG"
                     /locus_tag="WGO65_001516"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ0327799.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGNECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGKLKDVSDSALLAKLTAQSEAIAECYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTATN
                     AARFLNLDAITWTNTRVTLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATAEGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(3454..5292)
                     /gene="glmS"
                     /locus_tag="WGO65_001517"
     CDS             complement(3454..5292)
                     /gene="glmS"
                     /locus_tag="WGO65_001517"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ0327800.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKSGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATKGVHVI
                     RAPRHVGKLSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            5574..6092
                     /locus_tag="WGO65_001518"
     CDS             5574..6092
                     /locus_tag="WGO65_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327801.1"
                     /translation="MKQMLLAVGVVEVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT         1337 a         1469 c         1910 g         1547 t
ORIGIN      
        1 aaaacgacgg tgttgattag ggggtcgacc agcgcgaccg cgccagatac gcctatactg
       61 cccgtcagca cataggttac actgaaggcg gtggtgaaat gcagtgcggc aaaagtcagg
      121 gttttaagca tcatcctctc ccgaattgaa aatttacgga gagatgataa agattatcat
      181 caggccgtgc cggtttaaat ttgctattta ctgttagtgt aaataaatca attttaaagt
      241 aaggatagaa atgatgaatc acaaaaagat agtcgttctg gatgcggata ctttgcccgg
      301 cagggctttt cacttcgatt ttccgcacga gcttgcggtt tacggtacga caggtgcgga
      361 tgaaacggcg gaacgggtgc gcgatgcaca tattgtcatt accaataagg taatgatttc
      421 cgccgacatt attgcggcta atccgcagtt ggagctgatt gccgtcagtg cgaccggtgt
      481 gaacaatgtc gatattgggg cggcggaggc ggcgggcgtt gcggtatgca atgtccgcgc
      541 ctacggaaac gaatcggttg cggaacatgc ctttatgctg atgattgccc tgatgcggaa
      601 tttgcctgcc tatcagcgtg atgttgcggc aggattatgg gaaaaatcgc cgtttttctg
      661 ccattacggc gcgccgattc aggatttgaa cggcaaaacg ctggcggttt tcggacgcgg
      721 caatatcgga cggacgctgg caggatacgc gcaggcgttc gggatggggg tggtgtttgc
      781 cgaacacaaa cacgcgcccg ctgtgcgcga aggctatgtt gcctttgaaa atgcggtacg
      841 gactgccgat gtgttgtcgc tgcactgtcc gctgaacgca caaactgaaa atatgatagg
      901 cgaaaatgag ttgcggcaga tgaagcccgg tgcggttttg attaattgcg ggcgcggcgg
      961 gctggtggat gaaaacgcgc tgcttgccgc actcaaatac gggcagatcg gcggggcggg
     1021 cgtggatgtt ttgacgaatg agccgcctaa aaacggcaat cctctgttga aggcacgact
     1081 tcccaatctg attgttacgc cgcataccgc gtgggcaagc cgcgaggctt tggacaggct
     1141 gtttgatata ttgttggcga acattcacgc ctttgtgaaa ggcgaggcgc aaaaccgggt
     1201 ggtttgagct tgtcgggatg acgaaaaaat gccgtctgaa cgcctcaagg gttcagacgg
     1261 cattgttttg acatttctgc ttaaccgact ttgtcgcccg gttgtgcgcc tgcatccaca
     1321 tccaagagct tcagtttgcc ctctgccgtg gcggcactca aaatcatacc ttcggatacg
     1381 ccgaattttg ccattttgcg cggggcgaag ttggcgacgg cgatgaccat tcttccgttt
     1441 aactcggcag ggttgggata agacgcggcg ataccggaga agatgatgcg tttttcaaaa
     1501 ccgaaatcga ggtcgaattt caaaagtttg gtgctgccct cgacggcttc gcagttcaag
     1561 actttggcga cgcgcatatc aattttcata aagtcgtcga agctggcctg ttcggcgact
     1621 ttttcgtatt tgctctcttc ggcggcaggt gcggctgcgg cggcgatgct ttgtttgttg
     1681 gcttcgatta aatcgtccac ttgtttttgc tccactcgtt gcattaaatg tttgtatttg
     1741 ttgatggcgt gtttgcccaa ggttaccctt gtgttcgtcc aagtaatcgc gtccagattg
     1801 aggaaacgcg cggcattggt ggcggtttgc ggcaatacgg gggcgaggta ggcggtcagc
     1861 atcgtaaagg cgttgatgag ttcgctgcat acttcgtgca ggcgttcgtc ttggccttct
     1921 tgtttggcga gttcccaagg tttgttggca tcaacgtatt cattaacaat gtctgccaat
     1981 gccatgatgt cgcgcagggc gcgggcgtat tcgcggtttt cgtagcattc ggcaatggct
     2041 tcgctttgcg cagtcagttt tgccagcaat gcgctgtcgg aaacgtcttt gagttttcct
     2101 tcaaagcgtt tggcgatgaa acctgacgcg cgggcggcga tgttgacgta tttgccgacg
     2161 aggtcgctgt tgacgcgcga gataaagtct tgcaggttca aatcgatgtc ttcgattttg
     2221 ctgttgagtt tggcggcgat gtagtagcgc atccactcgg ggtttaggcc ttgttccaga
     2281 taggatttgg cggtaataaa cgtgccgcgc gatttggaca ttttttgtcc gtccacggtc
     2341 aaaaagccgt gtgcgtacac gccggtcggg gcgcggtggc cggagaaatg cagcatggcg
     2401 ggccagaaca gggcgtggaa atagaggatg tctttgccga tgaagtggta catctcggtt
     2461 tggctgccgg ctttgaagta ttcgtcgaaa tcgatgccga tgcggtcgca caggttttta
     2521 aacgatgcca tgtagccgac gggcgcgtcc agccagacgt agaagtattt gcccggcgcg
     2581 tcggggattt caaaaccgaa atacggcgcg tcgcgggaaa tgtcccagtc ggacagggtg
     2641 gtttcttcgc cttcgcccag ccattctttc attttgttga gggcttctgg ttgcagatgg
     2701 ggtttgccgt cgtgcgggtt gttgccggaa gtccatgctt tgaggaagtc ggcgcattcg
     2761 cccagtttga agaagaagtg ttcggattcg cgcaattcgg gtttcgcgcc ggaaacggcg
     2821 gaatacgggt taatcagttc ggtcggggaa taggtcgtgc cgcagacttc gcagttgtcg
     2881 ccgtattggt cttgggcgtg gcatttcggg cattcgcctt taacgaagcg gtcgggcagg
     2941 aacatttgtt tttcggggtc gaaaagctgc tcgatgacgc ggctctcaat cttgccgttg
     3001 gctttcagcg cgcggtaaat atcttgggaa aactgtttgt tttcagggga atgggtgctg
     3061 taataattgt cgtagccgat gttgaaaccg gtaaagtcgg cgaggtgttc ttcgcgcact
     3121 ttggcaatca tgtcttcggg cgcgatgcct tgtttttgcg cggcaagcat cacgggcgtg
     3181 ccgtgggtgt cgtcggcgca gcagtagtag cattcgttgc cgcgcagttt ttggaagcgc
     3241 acccaaacgt cggtttggat gtgttcgacc atgtggccga ggtggatgct gccgttggca
     3301 tagggcaggg cggaggtaac taagattttg cgtgtcatat tgtgctttgc aaacaatggg
     3361 taaaggcgga ttataccgca aatcaaacgg ggaaatgccg tctgaagcct gaaaaatcgg
     3421 gcttcagacg gcatttttgc aaaacggcgg gagttattcg acggttacgg atttcgccag
     3481 gttgcgcggc ttgtccacat cggtaccgcg tgcgagggcg gtgtggtagg cgaggagctg
     3541 cacggggata gtatgcacga cgggggacag tttgccgacg tggcgcggtg cgcggataac
     3601 gtgcacacct ttggtggcat taaaattact gtcgaggtcg gcaaagacga aaagttcgcc
     3661 gccgcgcgcg ccgacttcct gcatattggc tttgactttg tccaacaggc tgtcgttggg
     3721 tgcgatgacg acgacgggca tgttttcgtc caccaaggca agcggcccgt gcttcagttc
     3781 gccggcagga taggcttcgg cgtggatgta ggtgatttcc ttcagtttta acgcgccttc
     3841 gagggcaatc gggtaatgga tgccgcgccc caaaaacagc gcgctggttt tcttggcaaa
     3901 ctgttgcgcc caggcggcaa tttgcggttc gaggtttaaa gcgtgctgca cgctgccggg
     3961 aagctggcgg agttcttcgg tgtaacgtgc ttcgtcttct tcggaaacca aaccgcgcac
     4021 tttcgccagc gttaccgcca aaccgaacag cgcaaccagc tgcgtggtaa atgctttggt
     4081 tgaggcgacg ccgatttccg caccggcacg ggtataaagc acgaggctgc tttcgcgcgg
     4141 cagggcggat tccatcacgt tgcagatgga gaggctgtgg cggtgtccca aggattttgc
     4201 gtatttcaac gcctccatcg tgtccagcgt ttcgccggat tgggaaatgg taatgaccag
     4261 ttggtccgga tcggcaatca cgctgcggta tcggtattcg ctggcgattt cgacatcggc
     4321 cgggattttg gcgatggatt ccaaccaata tttggcggtc agcgcggcgt aataggacgt
     4381 gccgcaggca aggattttga cgctgcggat gctttcaaac acgcttttgg cgtttttgcc
     4441 gaagttttcg gggatgaagc cgccgtcgag gaaaacctcc gccgtgtctg caatcgcgcg
     4501 cggctgctca tggatttcct tttgcataaa gtggctgtac ggtcccagtt ccaaagaggc
     4561 gagcgagagt tcggatacct tgactttgcg ttcggcaggc aggccgcttt tatcggtcag
     4621 ccgtttgatg ccgtctgaag ccagcagcgc gatgtcgccg tcttcgaggt acgccacgcg
     4681 gcgcgtaaag gcgatgacgg cggatacgtc cgaagcgata aaggtttcat cgtcgcccaa
     4741 agcgaccaaa agcgggcagc ccatacgcgc cacaactaat tcatcaggct tgtcttgggc
     4801 aataaccgcg atggcgtatg cgccgtggaa acgtttgacc gcttcttgta ccgcttcaaa
     4861 cagcctgccg ccgttttgcg cgtattcgtg attgatgctg tgtgcgatga cttcggtatc
     4921 cgtttgcgat tcaaaacggt atcccaaacc ttccaagcgt ttgcgttcgc tttcaaagtt
     4981 ttcgatgatg ccgttgtgga cgaccgcaat catcccgccg ctgatgtgcg ggtgggcgtt
     5041 cggctcggta acgccgccgt gtgtcgccca gcgcgtatgc ccgatgccga tgccgccgct
     5101 gatgcctttt tcgcgtgccg cgtcctccat aagctgcacg cgtccgacgc ggcgcacgcg
     5161 tttgattttg ccgtcggtgt tgacggcaat gcctgatgag tcataacccc ggtattcgag
     5221 gcgtttgaga ccgtcggtca gaaaatcgac gacgttgtga tgggcgcgga tggcgccgac
     5281 gataccgcac ataactgttc cttagtatcc ggttgaaaaa aaacaggcgc ggacggctcc
     5341 cgtgccgcac cttccgcttc ggattataaa ccgcctcccg cgccgaaaaa cagcaaaatg
     5401 ccgtctgaag gcttgggctt gctcaaaaaa gggagggatt tccccgcctg accgggatgg
     5461 gcgttcagac ggcattgcct gctgccggtt tatagttttt acaaatcaat attgacaagc
     5521 tgaaaaaaaa aaacaatata ctcgctcggt cttaatgtta acggagtatg gaaatgaaac
     5581 aaatgctttt ggccgtcggc gtggtggagg tgttggcggg ctgcggcaag gatgccggcg
     5641 gttacgaggg ttattggcgc gaaaaatcgg acaaaaaaga gggtatgatt gccgtcaaaa
     5701 aagaaaaagg caattacttc cttaataaaa tcaacgtgtt tacaggtaag gaagaatcta
     5761 tgcttttgtc tgaaaaagac ggcgcgcttt cgataaacac ggggataggg gaaatcccga
     5821 tcaaactttc cgatgacggg aaagagctgt atgtcgaacg caggcagtat gtcaaaaccg
     5881 atgcggcgat gaaggacaaa atcatcgccc atcagaaaaa gtgcggacaa acggcacagg
     5941 catatctcga cgcgcgaaat gcgttgccgt caaaccagac gtatcagcag catcaggcgg
     6001 cgatcgagca gttgaaacgg cggtttgaag ccgagtttga cgaattggaa aaagaaatca
     6061 aatgcaacgg caaaccgaca ttgttgtttt agcgtaggac aaccgggagg atgccgccgt
     6121 ccgaatcgga cgtgcggttt ctgtaccggt acgggcgggc aggaatgtcc gctttttttg
     6181 ttcggacgcg tttgaatacc cgtttgattc cgtccgtttg caaagggtat ttttgttcgg
     6241 gcggaaatta tagtggatta aca
//
LOCUS       DAWFAN010000112         6262 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWFAN010000112 DAWFAN010000000
VERSION     DAWFAN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.112"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(391..825)
                     /locus_tag="WGO65_001519"
     CDS             complement(391..825)
                     /locus_tag="WGO65_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ0327802.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGO65_001520"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGO65_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ0327803.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WGO65_001521"
     CDS             complement(1556..2398)
                     /locus_tag="WGO65_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ0327804.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGIKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WGO65_001522"
     CDS             2653..3291
                     /locus_tag="WGO65_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ0327805.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WGO65_001523"
     CDS             complement(3743..4543)
                     /locus_tag="WGO65_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ0327806.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(5522..6163)
                     /locus_tag="WGO65_001524"
     CDS             complement(5522..6163)
                     /locus_tag="WGO65_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ0327807.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMMMSEKSGVPLYFDRAL"
BASE COUNT         1672 a         1568 c         1296 g         1726 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggcgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacaacccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 catcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttgccgct gccttctccg cgccccgttc cgtctccttt gataccggtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgcg gcaggcgcgc
     2221 ctgcaccttc gggtgcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctcg ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttta cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatctg gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg tggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctgctg cttatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaagtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt ggccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gtttgctgga taacaaagtc
     4741 caaaccaata gcagataaaa taaggcatcc accccctttc ttcattaagg ataagagata
     4801 tcgagaaaca aatcataact aaacagaaaa aattttggag ataaagccat ttcattcccc
     4861 tattcaagaa tctggccaag ataggtattt tgtatactct agaaaagaaa ggcatttcca
     4921 agggaaacat atcagataaa aacttttgtt tattttttac tatagataga accctatttc
     4981 tcaagataaa tccaaatgca atgccaatta tcattattaa taaacaaagg atagtataac
     5041 tatggataac cttcattaaa caaaaagaag aaatggcaga taaattacat acgatatagt
     5101 ggaatataaa atatttacgg tctaaacctt gttcagttgc aattttttta tacttacctt
     5161 gcgcaaaaaa atcaaagaaa tccattaaac tatctttcac attagaaatt taaaagctaa
     5221 ataatacaac aaacaatgtg aagtactatt catggtttat ttaaaaaata atactattct
     5281 gaacattatt tagatacaga aattaacaaa ttagaactaa acaagctttt aaatacttta
     5341 attttattgg aaagctataa aaggaactat aactttacac actagtcact tctttttaag
     5401 aggcaaaagg gattgggaag gtcgtcttgg agataagcac tggtatttcg gccaatggta
     5461 aatagagttt acctcaaata gggtagaacc tccttcatct gtcagttaat aacagccact
     5521 tttacaatgc cctgtcaaaa taaagcggca cgcccgattt ttcactcatc atcatcaaat
     5581 aacccatcac cttttgaggc cattcgatac cgcgcaccac ggtcagattc ctcaaaacgg
     5641 ggaaaaccaa aatatcctcc atgccgattc cgccgttgat gccgtctgaa gcaccgtcca
     5701 tcaaattctc caactcttgc aaatctgcgt ttatgcgttc gagatattgg gcggttttat
     5761 tcaaattggc ggaaaagctg ccgatgcttt tctctttttt gtctgtaaaa tattttaccg
     5821 cttccggcgt tgcaaattcg ggcagcccga ttttgatcac gcgcggctgc accagtttgt
     5881 cgttgtatcc gcccaccttg tccagccacg cccgtatctc ggggcggact tcgtctttca
     5941 gacggtcttc gcggtcgaaa tgccgcacaa tgtccaaact ctcgcccata aacgaaccgt
     6001 cttctttttg caggacgggc acttgtttcg caccgatcat accgatcggc gttgcctcgt
     6061 cgtcgtttgc cagcacggct tcttcaacgt ccgcgccaaa cagcccggca gccatccgcg
     6121 cacgcacaca aaacgggcaa tggtcgtaaa tatacagttt catcaaaata ttcctcgtca
     6181 acctgtcggt accggctacc ttaacacccc gcgccacccg aaacaagttt atcttcccgc
     6241 ctatgcaccg taaataaata ag
//
LOCUS       DAWFAN010000113         6236 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWFAN010000113 DAWFAN010000000
VERSION     DAWFAN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.113"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(1..201)
                     /locus_tag="WGO65_001525"
                     /pseudo
     CDS             complement(1..201)
                     /locus_tag="WGO65_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(403..777)
                     /locus_tag="WGO65_001526"
                     /pseudo
     CDS             complement(403..777)
                     /locus_tag="WGO65_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1083..2342)
                     /gene="rho"
                     /locus_tag="WGO65_001527"
     CDS             complement(1083..2342)
                     /gene="rho"
                     /locus_tag="WGO65_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ0327808.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTSNYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(2562..4946)
                     /gene="ppsA"
                     /locus_tag="WGO65_001528"
     CDS             complement(2562..4946)
                     /gene="ppsA"
                     /locus_tag="WGO65_001528"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ0327809.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSISDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATDLLSEGQE
                     VTVSCAEGDTGFIYSGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSIYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            5357..6178
                     /locus_tag="WGO65_001529"
     CDS             5357..6178
                     /locus_tag="WGO65_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ0327810.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1362 a         1449 c         1746 g         1679 t
ORIGIN      
        1 tttggtttgc gcttccgcca gcggacggtt gcggcaggct ttgcgcataa tgccgtcctg
       61 caactgatgt tcttccagat gttgccggtt ttccgcactg tcatagcctt tgtcggcata
      121 gacggtcgta cctttgggca gtccttccaa caacggcgac aggtgtttgc actcatgggc
      181 attggcggag gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
      241 acaaacagta cggaacccat tcacttggtg cttcagcacc ttagagaatc gttctctttg
      301 agctaaggcg aggcaccgcc gtactggttt ttgttaatcc actataaaaa tttatggggc
      361 tgtcctagat aactaggata aactcgattt tactaattgt tttcaaatgg aaatttgaac
      421 ttttatctca ctgttgttaa aacgtcgttc gtaccccttt aaatacagct caaaatgcgc
      481 tttgggaatg ccgtcaaact tgcgtaaatg acgttttgcc cggtttcaaa agttcccaat
      541 tccattgata tggttttgtc gttcagcaaa ataactttca tctgcttcta ctttgccgtc
      601 aaacatttcc aaatgcggac tgttttgata aataagtaat cgtaaacgat gaaaataata
      661 ggctgcggta tttttattaa cgcctactaa ctctgctgct gttcttgcag ttacacctgc
      721 gacaaacagt tcaatgagtt tattttgttt atactggctt agacgacttt ttctcatagg
      781 ggcaactcta acttaatttg aatttcccta gttatctagg acagccccaa atttatacaa
      841 aaatgagcgc ggttcggcgc aaccttgaat caagttcccg catcggtttt cattgccggt
      901 acggatgcgt tcaagccggt ttttcaaagg ccgcgctttc ggcaagcgga cacggacact
      961 gccgacggtt gcgccgttaa cggggggagg gaggagctgc gccgaccgtg tgaatgaaag
     1021 tgccgtctga aacccgattt tcaggcttca gacggcattt cgcattaatg cgggcggcgc
     1081 gtttatttgc cgcgcatcag ttcaaagaaa tcgtcgttgt ttttagaggc tttgattttc
     1141 ccgtttaaga actcggtagc ttcgatttca tccatcgggt gcaggaactt gcgtaagagc
     1201 cacatacgtt gcaactggtc gttcggcaca agcagctctt cgcggcgcgt gccggatttg
     1261 ttgatgttga tggcggggaa gaggcgtttt tccgccatac ggcgatcaag gtgcaattcc
     1321 atattgccgg tgcctttgaa ttcttcgtaa atcacatcgt ccatacggct gccggtttca
     1381 accaatgcgg tggcgataat ggtcagcgaa ccgccttctt ccacgttgcg cgccgcaccg
     1441 aagaaacgtt tggggcggtg cagcgcgttg gcatcgacac cgccggttag gattttgccc
     1501 gaggtaggca cgacggtatt gtaggcgcgg gcaaggcggg taatcgaatc cagcaggatg
     1561 accacgtctt ttttgtgttc taccatacgc ttggcttttt caagcaccat ttcggcaact
     1621 tggacgtggc gttgcgccgg ttcgtcaaag gtggaggaaa ccacttcgcc gcggacggaa
     1681 cggctcattt cggttacttc ttcgggacgc tcgtcaatca agaggacgat gagttcgact
     1741 tcgggatagt ttgaggtaac ggcgtgggca atgttttgca gcatcacggt tttaccgctt
     1801 ttgggcgggg caaccaagag ggcgcgctga cctttgccga taggggaaat caggtcgatg
     1861 gcacgtccgg tcaggttttc ttcggacttt aagtcgcgtt ccagcttcaa ctgttcggtc
     1921 ggaaacagcg gggtcaggtt ttcaaacagg attttatggc ggcatacttc cgggtggtcg
     1981 ccgttgatgg tatcaagcct gaccagggca aaatagcgtt cgttgtcttt tgggacgcgc
     2041 acgcttcctt cgatggtgtc gcccgtatgc aggttgaagc ggcggatttg ggtgggcgag
     2101 acatagatgt cgtcggggcc ggcaagatag gacgtgtccg cgctgcggag gaagccgaag
     2161 ccgtcgggca ggatttcaag cgtgccggag caggtgaaac cctcgccttt tttcatcatc
     2221 tggcggacga tggcaaatac gaggtcttgt ttgcggaatc ggttggcgtt ttcgatgccg
     2281 tgttcttccg ccaattctaa gagtttggaa atgtgcaggg tttgtaattc ggagacgtgc
     2341 ataataatga tgtattttga agaggaaaaa gacaggcaga tgccgtctga aagaagaagc
     2401 tgaccgttgc cggttgctcg gggaaagggg aattgtaggc agtcggcgcg tgggtgtcaa
     2461 atattatcgc ggacggggca tcggcaggaa atgccgtctg agcggagctg cttggaaaaa
     2521 aatacccccg cgcttttcag gctcgggggt atgggcattg attatttgtt caattcattc
     2581 gccaaataca gccaagtttc gatgacggta tccgggttca gggaaacgct ttcaatgcct
     2641 tcctcaacca gccatttggc gaagtccgga tggtcggacg ggccttgacc gcagatgccg
     2701 acatatttgt tctgcttgcg gcaggcggag atggcaaggt gcagcatcac tttgacggca
     2761 gggttgcgtt cgtcaaacga ttcggatacc aagccgctgt cgcggtcgag accgagggtc
     2821 agttgggtca tgtcgttcga gccgatggag aagccgtcga agtattgcag gaattgttcc
     2881 gccaataccg cgttgctcgg cagctcgcac atcataatca ggcgcaggcc gtttttgccg
     2941 cgttccaagc cgttttcttt cagggctttg acaacggctt cggcttcgcc caaggtgcgg
     3001 acgaacggaa tcatgatttc aacgttggtc aaccccattt catcgcggac gcgtttcaag
     3061 gctttgcact cgagtgcgaa gcagtctttg aagttatcgg ctacataacg cgctgcacca
     3121 cggaagccca gcattgggtt ttcttcgtgc ggttcgtaga tgctgccacc caccaagtta
     3181 gcgtactcgt tggatttgaa gtcggacata cggacgatgg ttttacgtgg gtaaaccgat
     3241 gcagccaatg ttgccacgcc ttctgcgatt ttatcgacgt agaagtcgac aggggacgca
     3301 tagccggcga tgcggcgggt aatttccgct ttcaactcat catcttgttt gtcaaattcc
     3361 aacaaggctt tggggtggat accgatttgg cggttgatga tgaattccat acgtgccaag
     3421 ccgatgcctt cgctcggcag attggcaaag ctgaatgcga gttcggggtt accgacgttc
     3481 atcattactt ttacaggtgc tttaggcatg ttgtccaaag caacatcggt aatttgtacg
     3541 tccaacaggc cggagtagat gaagccggta tcgccttcgg cacaagatac ggtaacttct
     3601 tgaccttcgg acaacagatc ggtagcgtcg ccacaaccta caacagccgg aatacccaat
     3661 tcacgcgcga taatcgctgc gtggcaggta cggccgccac ggttggtaac gatggcagaa
     3721 gcacgtttca ttaccggttc ccaatccggg tcggtcatgt cggtaacgag tacgtcgcct
     3781 gcttcaacgg aatccatctc ggaagcatct ttaatcaggc gtactttgcc ctgtccgact
     3841 ttttgaccga tggcgcggcc ttcgcacaag acggtttttt cgccgttgat agaaaagcgg
     3901 cgcaggttgc ggctgccttc ttcttgggat tttacggttt caggacgcgc ttgcaggatg
     3961 tagagtttgc catccaagcc gtcgcggccc cattcgatgt ccattgggcg gccgtagtgt
     4021 ttttcgatgg tcagcgcgta atgtgccaac tcggtaattt cttcgtcgga aatggagaag
     4081 cggttgcggt cttcctcggg gacatcgacg ttggttacgg atttaccggc ttccgctttg
     4141 tcggtaaaaa tcattttgat gtgtttcgaa cccatggttt tgcgcaggat ggcgggtttg
     4201 cctgctttca gtgtgggttt gaatacatag aattcgtcag ggtttaccgc gccttgtaca
     4261 acgttttcgc ccagaccgta agaggaggta acaaagacga cttgatcgta gccggattcg
     4321 gtgtcgaggg tgaacatcac acctgatgcg ccgctgtcgg aacgcaccat gcgttgaacg
     4381 ccggcggaaa gggcgacgat gtcgtgttcg aagcctttgt ggacgcggta ggaaatggcg
     4441 cggtcgttgt acagggaggc gaatacatgg tgcatcgctt ctttaacgtt ttccaatccg
     4501 ttgatgttca agaaggtttc ctgttggccg gcaaatgatg cgtcgggcag gtcttcggcg
     4561 gttgcggaag agcgtacggc aacggaaatg tccgcaccgc cggcatcggc aaccattttg
     4621 ttccatgccg cttcgatttc ggcatcgagc tgttcgggga agggcgtgtc caaaatccat
     4681 tggcggattt ctttgccgac gcgtgccagt tcggcaacgt cttcgacatc taattttgcc
     4741 agtgcggcgg aaatgcgttc gctcagaccg ttgtgtgcga ggaatgcgcg gtaggcatcg
     4801 gctgtggtgg caaagccgcc ggggacgcga acgccttttt cggtcagctg actgatcatt
     4861 tcgcccagcg aggcgttttt accgcccacg cgttcaacat ctgtcatacg caggttttca
     4921 aaccagatta cgtagttgtc ggccatttgt gtgtccaatc caaaatatgt taaaaaagaa
     4981 acaaatccgc gtgcttattt taagcgattc gttcctctgc tgtcatgtgt tttatccgtt
     5041 ttaaaatcat gatgccgtct gaaaaattgc ggtttcggcg tgtgtagcgg tttgaaactt
     5101 gcagccggta tacttctttt tttgggtatt ttctttgtaa aacaggtggt ttgaataggt
     5161 taatgttttt tctgtttgat ttttttgttt attttttaaa attttctgcc aaaaaatact
     5221 ttatataaat aattttattt caaaattata ttgtgtctgt ttgggtgtaa tccgaggtag
     5281 gcgtgctgcg ggtgctttcc ttgtgtctgc cgctgctgtt atgatgatat ttttattctg
     5341 tatttaagga tggaagatga gcagtccgcg ccatgtgttt tacatttccg accgtaccgg
     5401 tctgactgcc gagaatatcg gcgaggcttt gctgaaccag tttggcaatc tgtcgttcaa
     5461 acgccatacg catccgtttg tcgatacgcc ggaaaaggcg cgcgcggtgg tggagaaggt
     5521 caatcggagc cggcaggaaa acggtcagcg tccgattgcg tttgtcagtg tggttgatga
     5581 cgaaatccgt cggattatca aaggggcgga tgcttttcag attaatttct ttgagacttt
     5641 tttgggactg ttggagaaag aactcaatac cgaagccacg gcatccgagc aggggcatca
     5701 cagtatcggt aatacgaagc gttacgatgc gcgtatggag gcggtgaatt tctcattgaa
     5761 ccacgatgac ggggtcagcg ataagaacct tcaggaagcg gatgtaatct tgatgggtgt
     5821 atcgcgttcg ggcaaaacgc cgacctgcct ttacctcgcc ctgcaatacg gcatccgtgc
     5881 ggcaaactat ccgctgattc ccgacgattt ggaatcggcc gatctgccgc gtatggtcaa
     5941 gccttatagg gataagctgt tcgggttgac catccagccg gaacgtttgc aggccatccg
     6001 ccaagagcgc cgcccgaatt caacttatgc caaaatcgat acatgccgca gcgaggtggc
     6061 ggacgcgcag agtatgttca gacggcatgg cattccgttt gcgaatacga cggataaatc
     6121 ggttgaggaa ttggcggtac acatccttca ggcgtgcaag ctcaaacgca ggttttgacg
     6181 ggctttgatt cggtttgaag gcggaactgc cgtctgaaat caggtttcag acggca
//
LOCUS       DAWFAN010000114         6138 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWFAN010000114 DAWFAN010000000
VERSION     DAWFAN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.114"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            205..1638
                     /gene="lpdA"
                     /locus_tag="WGO65_001530"
     CDS             205..1638
                     /gene="lpdA"
                     /locus_tag="WGO65_001530"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0327811.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            1733..2020
                     /locus_tag="WGO65_001531"
     CDS             1733..2020
                     /locus_tag="WGO65_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327812.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            2119..3285
                     /gene="sucC"
                     /locus_tag="WGO65_001532"
     CDS             2119..3285
                     /gene="sucC"
                     /locus_tag="WGO65_001532"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ0327813.1"
                     /translation="MNLHEYQAKELLAGYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            3296..4186
                     /gene="sucD"
                     /locus_tag="WGO65_001533"
     CDS             3296..4186
                     /gene="sucD"
                     /locus_tag="WGO65_001533"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ0327814.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            4215..4430
                     /locus_tag="WGO65_001534"
     CDS             4215..4430
                     /locus_tag="WGO65_001534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327815.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFFPFWGSQRPLQNSPK
                     SPKFPPRHLGDFGEFCKGLGLSVPVCA"
     gene            complement(4737..>6138)
                     /locus_tag="WGO65_001535"
     CDS             complement(4737..>6138)
                     /locus_tag="WGO65_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ0327816.1"
                     /translation="FYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSIWKVILYL
                     LISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSKEAAEMIF
                     GKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDGIDIRPSQ
                     IPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGLQNQNTKL
                     QDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPWNAPNLHD
                     EYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQREYSIYL
                     LGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQTTSVEIPK
                     FMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTETEYLIFS
                     ELSKALDVGTPFKNKQ"
BASE COUNT         1753 a         1368 c         1407 g         1610 t
ORIGIN      
        1 gatgtagcgt aatgtagtat cgtgctacaa taggctcaac aaacgattga gaccgtctga
       61 aacatttgat tcgaatgaat cggcagatat ggactttcag acggcttttt cttaaaacca
      121 tcaaaacgca gtcattcaaa ataaaaaaga aacgaaaagt atcgttttta ttttgagata
      181 ctgctaaaag caaaggatga cacgatgtct caatatgatg tagtagtgat tggtgcaggc
      241 ccgggcggat acgttgccgc catccgtgcc gcacaactgg gtttcaaaac tgcctgtgtc
      301 gatgcaggcg ttaacaaagc aggcaatgcc cctgcattgg gcggtacttg cttgaacgta
      361 ggttgtatcc cttctaaagc cctgttgcaa tccagcgaac atttccacgc agcgcaacac
      421 gagtttgccg aacacggtat cactgtcggc gacgtaaaat tcgacgcggc caaaatgatt
      481 gagcgcaaag atgccatcgt gaccaaactg accggcggcg tgaaattcct gttccaaaaa
      541 aataaagtaa ccagtctgtt cggtacggct tcctttgccg gtaaaaatgg cgatgcttac
      601 caaatcgaag tcgataacaa aggagagaaa accgttatcg aagccaaaca cgtcatcgta
      661 gcgaccggtt ccgtaccgcg tccgctgcca caagtcgcta tcgacaatgt gaacgtattg
      721 gacaacgaag gcgcattgaa cctgaccgaa gtacctgcca aactcggcgt gatcggttcc
      781 ggtgtgattg gtttggaaat gggttccgta tggaaccgcg tgggtgcgga agttaccatt
      841 cttgaagccg cgccgacctt cctggctgcc gccgaccaac aaatcgccaa agaagccttc
      901 aaatacttca ccaaagagca aggtctgagc atcgaattgg gcgtgaaaat cggcgacatc
      961 aagtctgaag gcaaaggtgt ttccgttgct tacgaaactg ctgccggcga agccaaaacc
     1021 gaagtattcg acaaactgat cgttgccatc ggccgtattc caaacaccaa aggcctgaac
     1081 gcggaagccg taggcttgga aaaagacgag cgcggcttta tcaaagtaga tggcgaatgc
     1141 cgtaccaacc tgcctaacgt atgggcaatc ggcgacgtgg ttcgcggccc gatgttggca
     1201 cacaaagcca gcgacgaagg cgttgccgtt gccgaacgca ttgccggtca aaaaccgcat
     1261 atcgacttca acaacgtacc gttcgtgatt tacaccgatc ctgaaatcgc ttgggtgggt
     1321 aaaaccgaag agcagctcaa agccgaaggc gtggagtaca aaaaaggtac ttcaggtttt
     1381 ggtgcgaatg gtcgcgcatt ggcaatgggc aaagccaaag gtacggttaa agtgttggca
     1441 gacgccaaaa ccgaccgcat cttgggcgta cacatgattg gtccggttgt cagcgaattg
     1501 gttaccgaag gcgtgactgc gctcgaattc ttcgccagca gcgaagacat cgcccgcatt
     1561 atccatgccc acccaacctt gtccgaagtg gttcacgaag ctgcattggc ggccgacaaa
     1621 cgcgctttgc acggttgata gacattaagg ccttcgacaa attgaatgtt ccgagagctc
     1681 cgttttctga tttataattc cgtcagacaa acaaacagca tttacattcg ttatgaacaa
     1741 agaaatagtc ggtattttct ttataccgat gggcatcatc agcatgtgta tggccgcatt
     1801 gtggcagatg tatgtgatga tgaccgaaac ttatacgctc aaccgtttca aagataaaga
     1861 attggtttgg cgcgtggcat tgttgtttat cagtttcagc cttgccgttt atctgctctg
     1921 tccgaattcg cgtaaaaaag gcatcgtctt ttttattctc gggggaggcg gtgcagccat
     1981 gtatctgctg gcgcggatgt ggttgccttt cagcaagtga aacgacgatt ttccgaccgc
     2041 cgaaaggtag tctgaaacgc acgggcttgc catttggagg caaactcggg gcattccact
     2101 aatctaaagg agaaatccat gaatttacac gagtatcagg ctaaagaact gctggctggc
     2161 tacggtttgc ccgtacaagg cggtattttg gcacacaacg gcgaagaagc cgctgcagct
     2221 tacgacaaat tgggcggcaa attcgctgtt gtcaaagcac aagtacacgc cggcggccgc
     2281 ggtaaagcgg gcggcgtaaa agtcgttaaa agccgcgaag aagctaaaga agtggctgaa
     2341 agcctgattg gcaccaactt ggtaacttac caaaccgatg ccaacggcca acctgtcaac
     2401 agcgttttgg tttgcgaaga tatgtatccg gttcaaaccg agctgtactt gggcgcagtg
     2461 gttgaccgtt ctacccgccg cgttacattc atggcttcta ccgaaggcgg cgtggaaatc
     2521 gaaaaagttg ctgccgaaac tccagaaaaa atcttcaaag taaccgttga tccgctggtc
     2581 ggcctgcaac cttgccaagc tcgcgaagtt gccttccaac tgggcttgaa agacaaacaa
     2641 atcaacgagt tcgtcaaact gatgaccggc gcgtacaaag cgtttgtcga aaacgacttc
     2701 gccctgtttg aagtcaaccc gctggcagtt cgcgaaaacg gcgcactggc ttgcgtggac
     2761 ggcaaaatcg gcatcgacag caacgcgctc taccgcctgc caaaaatcgc tgaattgcgc
     2821 gacaaatctc aagaaaacga acgtgaactg aaagcttctg aattcgacct gaactatgtt
     2881 gccctggaag gcaacatcgg ctgtatggtt aacggtgccg gtttggcgat ggccaccatg
     2941 gacatcatca aactcaaagg cggtcaacct gccaacttct tggacgttgg cggcggcgca
     3001 accaaagacc gcgtggttga agcgttcaaa ctgattttgg aagacaaatc cgttaaaggc
     3061 gtattgatca acatcttcgg cggtatcgta cgttgcgaca tgattgcgga agccatcgtg
     3121 gcagccgtta aagaaatcaa cgtcgacgtt cctgtcgttg ttcgtttgga aggtaacaac
     3181 gccgaactcg gcgcgaaaat cctgaacgaa tcaggcctga aactgacttc tgcagacggc
     3241 ctgaatgacg cagccgaaaa aattgttgca gccgtaaacg cctaaggaga aaagaatgag
     3301 cgtattgatt aataaagaca ccaaagtatt ggttcaaggt ttcaccggta aaaacggtac
     3361 tttccactcc gaacaagctc tggcttacgg cactaaagtt gtcggcggcg ttaccccggg
     3421 caaaggcggt caaacccacc tgaacctgcc tgtattcaac accatgaaag aagctgttaa
     3481 agaaaccggc gcggatgcat ccgtaattta cgttcccgct ccgtttgtgt tggattctat
     3541 cgttgaagcc gttgattcag gcgtaggctt ggtcgttgtg attaccgaag gcgtacctac
     3601 tttggacatg ctcaaagcca aacgttactt ggaaaccaac ggcaacggta cccgcttggt
     3661 cggccctaac tgcccgggcg tgattactcc gggcgagtgc aaaatcggca ttatgccggg
     3721 ccacatccac actccgggcc gcatcggtat tatttcccgt tccggtacat tgacttacga
     3781 agccgtggca caaaccacca aactgggctt gggtcaatca acctgtatcg gtatcggcgg
     3841 cgacccgatt cctggtatga accaaatcga cgcactgaaa ctcttccaag aagacccaga
     3901 taccgacgcc atcatcatga ttggtgaaat cggcggtact gcggaagaag aagcagccga
     3961 atacatccaa tccaacgtaa ccaaacctgt tgtaggttac attgctggtg ttaccgctcc
     4021 taaaggcaaa cgtatgggcc acgccggtgc gattatttcc ggcggtaaag gtactgccga
     4081 agaaaaattc gccgctttcg aaaaagccgg tatcgcctat actcgcagcc ctgccgagtt
     4141 gggcacgacg atgctggaag tgttgaaggc aaaaggtttg gcataatcag gtttgacaac
     4201 tgattaaaca tcaaatgccg tctgaaaccg gaaatcgggt ttcagacggc attttgtttg
     4261 tcatttcaaa aagaggcagc ctcaacatac ccacattatt ttttcccttt tggggcagtc
     4321 agagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat ttaggggatt
     4381 ttggggaatt ttgcaaaggt ctcgggctaa gtgtgcctgt ttgcgcctaa aaggcggccc
     4441 ggatgcctga ttatcgggta tcctgggagg attaaggggg tattggggta aaattagtgg
     4501 atatttgaaa cgaaaacagc cgaaaacctg tgtttgggtt tcggctgtcg ggagggaaag
     4561 gaattttgca aagctctcgt attggctttg aagttccgtg taattcacag gtagggcgtg
     4621 tggcacagcc acgcacgcgg tcggttgggt atgcaggcta cggctttctc tgttgaaact
     4681 gcaacacgtc agctaccaac cgtgtgcgta gcgcacacgg ttggttggga tacaagttac
     4741 tgtttgttct taaatggagt accaacatca agggcttttg ataattctga aaatattaaa
     4801 tattcagttt cagtttttat tttaggagaa atatttgcat aatttctatc tttaaagcac
     4861 caatggatat atggtttcgt ttcatcttct gggttatcta aataagcaat aacattcaag
     4921 tcaaataaaa attgcaaaaa ctcattagca gtactcataa atttaggtat ttccactgat
     4981 gttgtttgta agtgcttttt caaacgttca aatgctttta aaaaatcact atatttaaat
     5041 ctatctttcc cgtttaaaaa ttcaaaaaat ttcaggaaat tttgataatc actttgacta
     5101 taataaaaca aaagatgatc tttgatttca ccaagtaaat atatcgagta ttctctttga
     5161 aaagaagtat tatcaaaatc ttctgctacg acataatctt ccttactttt cttatttttt
     5221 tgtagcaaag taagcatctg aagaatatcg cgaggtcgat aatacgattt tcttaggaag
     5281 ctaataaatg aagttaaatt tttatactca tcatgtaaat taggagcatt ccatggaaaa
     5341 taataatccc atgagttgcc tttttctaaa ctatcttgtt tttcttgctg ggttctcaaa
     5401 agatgatcaa aaacgccaaa aatctttgaa cttctataag atttataatc cgtcctccag
     5461 tctaaaaata ctgaattatc ttgaagtttg gtattttgat tttgtaaacc taatgaatca
     5521 aagatatcag gtctaatcaa taacacaact ctcatccttc ccttactatc tttaatggaa
     5581 gggaagatat cattatttaa catccatatg gcgttagcaa gaccttttac acactcatga
     5641 tattcatcaa atggaatctg tgatggtcta atatctatcc catcaataaa caaaatatga
     5701 ttatctttta gctttaactg agataaagca tctttaaatt ttctttcaat aaaacctaaa
     5761 tttgcttgga atttactttc tgtaaaagtt atttgttggg attcctcttc acctagttta
     5821 acaaattttc caaaaatcat ttccgcagct tcttttgaat tttctattaa agttattgct
     5881 tgtacaattt ccggatcaaa agcgccataa taatattcat ttatagcctc atctaaggct
     5941 ttaaatttat taaatattga agataatatt ccgttttctt tacatttgat ttgatttgat
     6001 atcaacagat ataaaatgac tttccaaata cttgtaaaat ctgaaacagt taagtgtctt
     6061 gctttcttta gctgaataaa ttttgaataa tcggtttcac gaacaaactt agtagtggca
     6121 tgtatgtttt tatagaag
//
LOCUS       DAWFAN010000115         6087 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWFAN010000115 DAWFAN010000000
VERSION     DAWFAN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.115"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            57..1610
                     /locus_tag="WGO65_001536"
     CDS             57..1610
                     /locus_tag="WGO65_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ0327817.1"
                     /translation="MNRTYANFYGMLAAACRKNGNGTAVFEGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGKSRPVGETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGVPAIYSAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEVKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(1700..3727)
                     /locus_tag="WGO65_001537"
     CDS             complement(1700..3727)
                     /locus_tag="WGO65_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ0327818.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLAVLVLTVPDQAQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEIGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            4016..6022
                     /locus_tag="WGO65_001538"
     CDS             4016..6022
                     /locus_tag="WGO65_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ0327819.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
BASE COUNT         1554 a         1853 c         1424 g         1256 t
ORIGIN      
        1 cggcatattg ttttcaaatg agggcgttcg ccgtccgcaa ccataaagga agtttcatga
       61 accggactta tgccaatttc tacggaatgc tcgccgccgc ctgccgcaaa aacggcaacg
      121 gcacggcagt gttcgaaggc aaggaaaaaa ccgcctaccg cgcactcaag caggaagccg
      181 aagccgttgc ggcgtatctg caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg
      241 cggtttccaa ttccacagaa tttattaccg cctatttcgc cgtctccgcc atcggcgcgg
      301 tcgccgtacc gatgaacaca tttttgaaaa acagcgaata cgcgtatatc ctgaacgact
      361 gcaaggcgcg cttcctgttc gcctcggcgg gcctgtcaaa agaattggcg ggcttgaagg
      421 cgcaaacgcc cgtcgaaaaa atcatttgga cgggcaaaag ccggccggtc ggcgaaacgg
      481 cggaaggcga tgcctttttt gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc
      541 gacaaccccg gataaatgat ttggcacaca tcatctacac ctccggcacg acggggcatc
      601 ccaaaggcgc gctaatcagt tacgccaacc tgtttgccaa cctgaacggc atcgaacgca
      661 tctttaaaat ttccaaacgc gaccgcttta tcgttttcct gccgatgttc cacagcttca
      721 cgctgacggc tatggtgctg ctgccgattt atatggcgtg ttcgattatt ttggtcaaat
      781 ccgtttttcc gttttccaac gttttgaaac agacactgct caaacgcgcg accgtgtttt
      841 tgggcgtacc cgcgatttac agcgcgatga gcaaggcgaa aatcccttgg tatttcagat
      901 ggttcaaccg catccgcctg tttatcagcg gcggtgcgcc tttggcggaa caaaccatcc
      961 tcgatttcaa agccaagttc ccccgcgcca aattgctgga aggctacgga ctgagcgaag
     1021 cctcacccgt cgtcgccgtc aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc
     1081 ccctgcccgg tttggaagtc aaagccgtcg atgaagaact ggtcgaagtg ccgcgcggcg
     1141 aagtgggcga actgattgtc aggggcggtt cggtgatgcg gggctacctc aatatgcctg
     1201 ccgccaccga tgaaaccatc gtcaacggct ggttgaaaac gggcgatttc gttaccatag
     1261 acgaagacgg ctttatcttt atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc
     1321 aaaatgtcta tccgcgcgag attgaagaag aaatctacaa actcgatgcc gtcgaagccg
     1381 ccgccgtcat cggcgtgaaa gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat
     1441 tgaaggaagg tatggatttg ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg
     1501 caaatttcaa aatccccaaa caaatccact ttaaagacgg gctgccgcgc aacgctacgg
     1561 gcaaggtatt gaaacgggtg ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc
     1621 gtccgaagcc ccgtccggca aaaaaaatgc ggtgaatctg cttcaccgca ttttttggca
     1681 aacgccggag ataaggcact tactctgcca attccacctg ctcgtgcgcc atcagttcct
     1741 gaccgacatc atccaacaac atcaaataac gttcgtagtt tttcaaaatg tcggcaatca
     1801 gctcgtcctt gttcatatac tgtacatcgt acccgacgcg cccgtcgaaa aaataagcgt
     1861 agggtttgta agttgtctga tgccggatat gcggcagctt gccgtcgtta atcaactggt
     1921 cggatacatc ctgcccgaca gacttaatcc cgtacataaa atcgcgcatc gtctctttcc
     1981 gaatgacgaa ctcgattgcg ggctcgtcct gatgaaacat cttatcgacc cggacgctca
     2041 agccgtattc ttccgaaagc tcccgttgca actcgtgcat agcgggcgat gcggtatgtt
     2101 tgaggaattt taaaatatcc tgctcctgcg tctggctcat tatctgcacc agccgttctt
     2161 tccacttgcc gcccgtccaa aatacactgg tagggttaac ccgggtctca aaatatttct
     2221 tatccgcact caatcctttc cacaggctga aacacattat cagcatcagc agggcaaacg
     2281 gcagggaaac aatcagggtc atagactgca ggttgccgag tccgcccgag cgcatcagca
     2341 aaacggcaac ggcagacatc agcacgcccc acataaccgc ctgccaccgt ggcgcgctca
     2401 agcccttgtc ccgagaggta atattgttca ggacataaat cccggaatcg gcagaagtta
     2461 caaaaaacag agaaatgacc agcaggctga cgatactcgt caattcgggc agagggaggt
     2521 aattaaagaa tttaaaaagc agcgtttccg gagaggaggt catcttttcg agcattcccc
     2581 ccgcaacccc gtcattcagc caaatcgccg tattgccgaa gacggtaaac cacaaaacgc
     2641 cgaacaggcc ggggatgagc aaaaccccga agacaaactc gcggatggtg cgcccctttg
     2701 aaatgcgcgc gataaacaaa cccacaaacg gcgcccaaga acaccaccac gcccaataaa
     2761 gcaccgtcca agattcaaac cacggcttgt gttcccgttc gtacgcataa gttttaaaac
     2821 tgaggcgcac cagatttccg aggtagttcc ctatgttgtc gccgaatgcc gacaacaggt
     2881 aaacagtggg acccgccgcc aaaacaaaaa acagcagcaa aaacgcaagg ctcaggttca
     2941 actcgctcaa caccttcacg cccttcccca cgccggatat tgccgaaacg acggcgaggg
     3001 acatgacggc ggcgataatc aaaacctgca cgctgaagct gttttcggca atccagccta
     3061 tttcctgcaa tccggcgccc agttgcgaag ccccgaaccc caatgtggtg atgatgccga
     3121 aaaaagtagc aagcaacgcc ataatatcaa tggcatcgcc gaaccttccg gaaatttttt
     3181 ctttcaacag ggggtaaaaa caagaacgca gggcaagcgg cagcttgtag cggaaaccga
     3241 aataagccaa agccaatgca atcgtaccgt acaccgacca agcgtgaacg ccccaatgga
     3301 acaccgtgtg cagcaatgcc tgctgctgcc tgtgttccgg cgtgccggcc gtaatgtccg
     3361 aaaaataatg catcaacggc tctgccacgc cgaaaaacat cagacccacg cccatcccgg
     3421 ccgcaaacag catcgccagc cacgacagga agccgaattc cggcacatct tcatcccgtc
     3481 cgagcctgat gtttcccaaa ctgctgaccg agagtatcag caggaaaccc agaaaaatgg
     3541 aaaacgttaa aacataaaac cagctgaact cggtaaaaat gacttctttt gcccgatcga
     3601 gccacatctg cgcctgatcc ggcacggtta aaaccaatac cgccaaaaca cacacaaaaa
     3661 acaaagtcgt caaaataacc atcggattaa atgacgttcg gcgttctata aattcagaca
     3721 gggacaaacc ttctcactcc tttgttaaaa acagacaaac ccggtcatcg ggcaaagcgg
     3781 tcaaaacctg ccggtaaaat accggcttcc ggatgacgaa atgcacaaac cggccgaaat
     3841 tgttataatc gaaaaaatta aaaatcaata cggattattc tatcagattg atttatcgat
     3901 atttattata tcattaatat agtttgattt caaaccggcg gaaaatgcgc cgcccaccgc
     3961 aggcgcgccc tccccgccat cggggcgttt aacgccggat tgccgatgcg gtacaatggc
     4021 tgatatgaag aaaattaccc ctcaaaacct acgccccctg ctttcggaaa gcttgggaca
     4081 taccgatttt gtcaacgtcc tcaacgcact gattaaattt ttgcgccgtg gcggcaaaaa
     4141 atgtgcgggg gaacgtttcg acctgattat cgacacattc aaacaagaca gggaattact
     4201 gtcccgcttc agccggtgtt tttacatctg gctcgcgcaa atacacattt atccggcact
     4261 catcaaactc ggcatcttct cgcgccacag ctttgcccgg gaaatgggca tacgcatcta
     4321 cgaacgcttc agcccgtcat ataaagattt tgccaacttg ggcgaagtct tcctttatct
     4381 tttccattcc gaaaacgacg acaaatggct gcaaacgctc aatatccgcc aatggctggt
     4441 tttatacgaa ctcatccgga gccacgccga gccgtccaaa ttgcagacgg cgggcatccg
     4501 ccttgccgat gcgcgtttgc gcgccatcga aatgctgtct gtctggacgg catccgaagc
     4561 catcgaaccc gacctcatcc gcatcgcccc gcgcctgctg gaagccgatt cttccttcgt
     4621 cgccctccaa cgcgaaaccg ccaaactggt cgaacactac cgcaacggca ccacgcctta
     4681 cgacaccgcc cacctcgaag tgatgttcga ccaatgtttc agccagattg actatttgcg
     4741 ccgcaaaggg acgggcgccg gctccggttc gtcggtcaaa gtcgcccacc tgctcgaacg
     4801 gctccggcag accgtagacc gtctgaagct gctcaccgac atccaaaccg gcgccggcaa
     4861 cagcaaccgc ctgaccatcg cgctgatgaa ctccctcatc tacgcggcgg tcgaacaata
     4921 cagcacccgc cacctgcgcc gcagcagcat ccgtatgctc gcccgcagca ttaccgaaaa
     4981 caaaagccac cacggcgaac actacatcac ccgcaaccgc aaagaatatt tcaaaatgtt
     5041 ctactcggcg gcaggcggcg gcatcatcat cgccctgatg gcgctgctca aaatccgcat
     5101 cggcacactc ggcctcagcc ccttcctgac ttccttgtcg gctgggttca actacggcat
     5161 cggctttatg atcatccata tgctgcactg caccgtcgcc accaaacagc ccgcgatgac
     5221 tgccgccagc tttgccgaac aggtcgatct caacgaaggc ggcaaagcgg tggacaacaa
     5281 actcgccaag ctcctcatcg acgtatgccg ttcccaaagt gtcgccgttt tcggcaacgt
     5341 ttccatcgcc atccttttgg cgtgcgccat atcgttcggc tatgcccatc tgtaccggca
     5401 gcccatactc gatgcccaca ccgccgccta ccagttcaaa tccatagaca tcatcgctta
     5461 cccgacgctg tggtatgccg ccattgcagg tctatggctg ttctgctccg gcatcatcgc
     5521 aggttttttc gacaaccgcg ccgactacct caacctgcgc caacgcctgc ccttcaaccc
     5581 cttgctgcgt aaaatcatgc gccccgggcc ccgccgcgtc ctcgccgcct acatccacaa
     5641 acactacggc tcgttggcag gcaacttcat cttcgggatg ctcttgggta tgaccggcta
     5701 tttcggacac ctcctcgggc tgccgctgga catccgccac gtcgcctttt cctccgccaa
     5761 cctcggctat gccgccgtca gcggcaacgt cggtttgggc acgttcgtac tcggcatttt
     5821 cagcgtcctc gccatcggcc tggtcaacct ctgcgtcagc ttcagcctcg ccctcttcgt
     5881 cgccctgcgc tcgcgcggca cgaaaatcgg cagcatccgc aatctgatta agagtttttg
     5941 gaatcagatt aaaagcaatc cctgcatact tttcctcccg cccgccaaag aacagggaca
     6001 tcctccttcg gacaagcctt gaccggcaat gccgtctgaa gcgggattcg ccccgaatac
     6061 cgccctgatg cgggaaatcc ccataaa
//
LOCUS       DAWFAN010000116         6023 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWFAN010000116 DAWFAN010000000
VERSION     DAWFAN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.116"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WGO65_001539"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WGO65_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ0327820.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WGO65_001540"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WGO65_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ0327821.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WGO65_001541"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WGO65_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ0327822.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIASITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1357 a         1708 c         1619 g         1339 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcaccct ccgccgcata gctgctgcgt
      181 tccgcatcga gcaagtcgag cgcgccggat acgccgtgct tgtaacgcag gccgaccaag
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccagc
      301 tgctcgcgcg ccgccaatgc gtttgccacg tcttgaaatg cggattggac ggcggattca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat caaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatagac ggcgcgaacg accaaacgcc cgtgccgctt
      481 ttgaacagcc cacccaattc ggcagaaccc gtaccgacgg ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcagcacgga tatcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagcttc
      661 tcaacaaaaa actgcttgtc caacggcaaa ccggcgggca ggtcgtcggg tatcggttgg
      721 ttaatcaggg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat tagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcgtaa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagccg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatac ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcgtgcc aacccaaatc gacggcgcgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg tgggcgttca
     1501 gacggcatgg ttgcttaatg ttggctgtcg tccgaaccgg tgatgcccgc ttcggcagcg
     1561 tgttttactg ccatttcgtg ttcgtgcgcg gtttctttga agaatttgcg caccaccaca
     1621 tagaaaagcg gaacaaggaa caccgacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gttgtaccaa tggcgcgctg gctggcagaa cttgcaccgc cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcaaa cgaggtcatg ataatcggac ggaaacgcag gcgggctgct
     1801 tccaaagcgg cttcaaccgc gcttttccct tgcgcttgaa ggtctttggc aaattcgata
     1861 atcaaaatcg catttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagtcctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccattcgaag
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cgcttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gcggcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccagcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattattg cggtcttgca ggttgatgct caggcccgaa
     2701 ccattgccca actccagaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgc
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga atgaagtcgg caggcgcata
     3061 aacaggaaca cgcccacaac cgccaaaccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactattgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaaag cggagaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcgcccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcac ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccagcac catcgcttcg attaaagtgt gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggaggatgac gttgccgaat tcttctgccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgca ctgccgatat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgcaac tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcggaag tggacaaata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcacccg gataagtggc gtgcaaagta atggtcgggg cggcgacaga cggatattgc
     4621 gaaacaggca ggcttttgat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcaaaaa tagggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgccgga ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ctggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcaacc gttacctcgc ggggttccat accgccttgg gcattcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ccgcctgctg cggcacaaca aatgcgttat
     5041 ccaccgccac ttggtccatc agcacgcgca catacaggcc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg gtcggatcgg
     5161 caaacagcag gcggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc aacagtttgc cttcggctat ctgacggcgc aatttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttggac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt aatgccggca gatttgattg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtatcgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgcgcgctt tccagacctg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgtcc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg atgatgccgc cgacttgggc gcggacatcg gcggtacgca
     5761 gcgattccaa acgccccggc aactcgacgg tcaacgcgac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggcgca ggggcttccc gaccagcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc att
//
LOCUS       DAWFAN010000117         6002 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWFAN010000117 DAWFAN010000000
VERSION     DAWFAN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.117"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(53..361)
                     /locus_tag="WGO65_001542"
     CDS             complement(53..361)
                     /locus_tag="WGO65_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327823.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WGO65_001543"
     CDS             complement(345..647)
                     /locus_tag="WGO65_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ0327824.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WGO65_001544"
     CDS             complement(721..3084)
                     /locus_tag="WGO65_001544"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ0327825.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     CVFEIARVFSKGSDGQFVQNERIGGLWYGAVMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGETRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3120..3638)
                     /locus_tag="WGO65_001545"
     CDS             complement(3120..3638)
                     /locus_tag="WGO65_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327826.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVLLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3645..4709)
                     /locus_tag="WGO65_001546"
     CDS             complement(3645..4709)
                     /locus_tag="WGO65_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0327827.1"
                     /translation="MIKSFELEKFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQATWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WGO65_001547"
     CDS             complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WGO65_001547"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0327828.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
BASE COUNT         1518 a         1546 c         1267 g         1671 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg atcatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgactgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgtttccccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cgcgtccggg atgcagggca ggatgtccgg ttttaacgaa
     1201 ctcgactgct ttgtttttca acagattttc cacatctgcc ttgatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttccgg catgaccgcg ccgtaccaca atccgccgat
     1321 gcgttcgttt tggacaaact ggccgtctga acctttgctg aacacgcggg cgatttcaaa
     1381 cacgcacacg cggttttgtt tgcggttcag attgttttgc agaatttcca ccaagccgcc
     1441 gatgagcgtg gaacgcatca cggcatactg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtaa ccgcgagccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggcccaa
     1861 acgcaatccg acctgcttcg cttcgggcaa ttcgccttgc gcttccacca tttcgcctgc
     1921 cgcaccgccg caaatctgca acaccaattc ggtagcacgc tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatcgg gagtagccgc
     2401 tttcgcattg acattttcaa taacgcggct gataaaacgg ccgcaatcgg caggcgcgtt
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacaccacgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tacccggcaa aacggcaccc ggcagcgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacgatctga accaacccac ccgtacccgc
     2881 atcgacttgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaacaacc acgcccgcaa acgcaggcgc ggcagtctcg gcttcttcca cttccaagcc
     3001 ggacatggtt aacagatgtt ccagcttatc ggaggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgag taggagaatt gcatgttatg ttctcaatta actaattaac tttagatttt
     3121 caagagctag ctaaaccttt ttgaatggct aagtccctat taattacttc tacagaaatg
     3181 ttccataaag aaatcaaccg caaagtatct ttcttattaa attcttgctt tattaattca
     3241 ttcaattgcc caagataatt aagcagcaca tccgggataa ccagtaataa tttgatttct
     3301 atgctacccc attgcaaatt tgaaaaagca ctaggcataa ttgtttcttt gatatcttct
     3361 ctgcctaata ttcccaaaat ttcaacattt aatgcattac accatttttg ataaatctct
     3421 gaaatatatt catcaaaacg ctcaggagat ctttcttgat ttggaatact ttgttttgcc
     3481 tcaacaaata acaaattatc ttcttgcaga tagataaact ctactgtctt aataccttct
     3541 tgccttctct tccggatata tggttctata taaaacaggt tattttttac tagattaaaa
     3601 gttatacctg attcagtgag atttattatt tcactcataa aaacctaacc aatccatgct
     3661 gtttccggag ataaaacacc tatttcttct ctgaacaatc gaatagactc accaataatt
     3721 tcattatccg gcatgctatt ttgtaaattt tcctgcaccc aagtagcctg ttccttttct
     3781 ttgccaggca taaaaacagg aatactttca ctatgctttc tagctaataa acacagcttc
     3841 ttgataacaa aatatgaatg cgtactcata aaaatctgta ttcccaattt ggatttagat
     3901 aaggaaaaca gaatttctag aaattgatta attgctttcg gatgtagatt agcttctggc
     3961 tcatcaataa ataagattga tccaggactc aaaaaccgat ttcctaatag agtgtctaat
     4021 atcccaatct tttttacccc ctctgctgta gaattgatag agaaagtttg tctatctttt
     4081 tcatataccc atgccttctt tttatcattg tagctaacat aaccgccaaa caatgcttct
     4141 agatcctttc tagcttccgc tgcctcaggg taattcctcc cttttgttgg tttaatatcc
     4201 aatgctttta ccaaatcaat gtaagtagca tcaaaaccaa atctcttttc ttcctctgat
     4261 tttttaatta catcaaatag tgatagaact tctttgggtg gtaaaaaaat agagttagcg
     4321 tttattctag gagaaagttc gtatagctcc ggtttaatta gctttttggt agttctacca
     4381 aaagaaaaca aaactgaaga actatcttct aaagtcatac ttaacttaag aggccgttcg
     4441 tttggatttt cttttcctct tgtaactaaa tcaccaattt cctctacttg gaacgtccaa
     4501 tatagcttat ctcgcaattc ctcagaaaaa tcacgaatat tctttccttt atgagcttcc
     4561 tcatgacttt ttaaaacact atataatgcc tttaaaataa aagttttccc tttgctattt
     4621 tctgcaataa ttaaatttat tttccctaaa ttatcagcct tcaataagct aatcggtccg
     4681 aatttttcaa gctcaaatga tttaatcata taatccccct aatatatatc aacaatattt
     4741 gtacaagcca cattgtaaaa agacagcaac cacagcacca tagtaaacgc atagcaggcg
     4801 gataatggag cgacatagcg tttcatgatt tagctttctg aggtaggcac aggctgcttt
     4861 tttcattttt aggcagccta cacaatcatt ttacaaactg cttcaaaaaa ttcaaatcgt
     4921 tatcaaaaaa caagcgcaaa tcgttcacgt tgtaacgcag catcgcgaaa cggtcgagac
     4981 cgataccgaa agcgaaaccg gtgtattttt cagaatcgat attcacattt ttcaacacgt
     5041 tgggatgcac catgccgcag ccgccgactt ccagccattt gccgttttcg cccataatgt
     5101 cgatttcggc agacggttcg gtgaacggga agaaagacgg gcggaaacgt acttgcaaat
     5161 catcgcgttc aaagaagcga cggataaaat ccgtgaacac tgctttcaaa tcggcaaaag
     5221 ttacgccctc ttctacccac aaaccttccg cctgatggaa cataggcgag tgcgtggcat
     5281 cgctgtccac acggtaaacg cggccggggg cgataatgcg gatgggcggc tcttttttat
     5341 cgagcatata gcggatttga atcggggaag tgtgcgtacg caaaacatcg ccgttttcaa
     5401 cgtaaaacgt atcctgcatc gcacgggcag gatggtttgc agggatgttc agggcttgga
     5461 aattgtgaaa atcgtcttcg atttcaggcc cgtccgccac ttcgaaaccc attccgtgaa
     5521 agagttcgac cacacgttgc aaggtcaggg ttacgggatg caggctgccg ccttcctgag
     5581 cgcgtccggg cagggtaata tcgagggctt cggcggcaag tcgggcttgc agcttgactt
     5641 cgttgagggc atcgcgtttg gcattaaaag ccgtctgaaa ccggtttttg cattcattga
     5701 tatgcgcacc tatggttttg cgctcttcag gcgacatttg ccccaaagtt ttcagaagtc
     5761 cggtcaactc gccggttttg ccaagataac gggctttgat ttgttctaga gcgttgaagt
     5821 cttgcgcagc ttctactgcg gcaatgcctt ctgcaacgat gcggtttaca ttttccataa
     5881 tatcaagtct gtcaattaat gatatgccac ctgaaacaac gaggccgcct gtatagcctg
     5941 acatatcaga ctcttcagac gaccttattt tcttcacggt caatccattg gataaatttt
     6001 ct
//
LOCUS       DAWFAN010000118         5989 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWFAN010000118 DAWFAN010000000
VERSION     DAWFAN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.118"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            59..505
                     /gene="yciA"
                     /locus_tag="WGO65_001548"
     CDS             59..505
                     /gene="yciA"
                     /locus_tag="WGO65_001548"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ0327829.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
     gene            complement(556..1377)
                     /locus_tag="WGO65_001549"
     CDS             complement(556..1377)
                     /locus_tag="WGO65_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0327830.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFGDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1496..1954
                     /locus_tag="WGO65_001550"
     CDS             1496..1954
                     /locus_tag="WGO65_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0327831.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKGEEPYDVEKFKQAAASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2118..3233)
                     /gene="lst"
                     /locus_tag="WGO65_001551"
     CDS             complement(2118..3233)
                     /gene="lst"
                     /locus_tag="WGO65_001551"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ0327832.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNHGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFKQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3334..3438
                     /locus_tag="WGO65_001552"
                     /pseudo
     CDS             3334..3438
                     /locus_tag="WGO65_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            3666..5891
                     /locus_tag="WGO65_001553"
     CDS             3666..5891
                     /locus_tag="WGO65_001553"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ0327833.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTDGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1611 a         1748 c         1334 g         1296 t
ORIGIN      
        1 aacgccgtac tggtttaaat ttaatccact atatatttca ataaaacaag gaaactttat
       61 gcagcacgaa gaaggcaacc gccaacgccc tcaaggcgaa ctgctcctgc gtaccgtcgc
      121 tatgccgcgc gataccaatc ccaaccaaga cattttcggc ggctggatta tgtcgcagat
      181 ggatttgggc ggcggcatat tggcggcgga aatcgcgcgg ggacgcatcg ttaccgtcgc
      241 cgttcaggaa atgaacttta tccgcccggt caaggtcggc aacgtcgtct gctgctacgg
      301 gcattgcgtc cgcgtgggca atacttccct ccagcttaaa atcgaagtct gggtgaaaac
      361 tttgatgaac gattgcgtta ccgaagaccg ctacctcgta accgaagccg tgttcactta
      421 tgtcgccatc gatgcggaag gcaatccgcg cccgattccg aaagaaggca accccaaatt
      481 ggcgggctta ttgcctactc cgtaaaaatg cccgtaaaaa tgccgtctga aactgtcttc
      541 agacggcatt ctgcatcatt tgaaccaaga cctgatgcgg gaaaacagcg acatactttg
      601 tttttcaaag tcttcccgca cttcgggcac ggcttttttt atcactttca ttcctgcaaa
      661 agtctgcgcc acgggcataa tttcaatcgt gttcacattc atatgcgccg gccggcggta
      721 cagccacaat gcggtttccg caatatcttc ggggcggata aattccacac cctcatacac
      781 gcctgctgcc ttctcgtcat cgcctttgaa gcgcacgttg gaaaactccg tattgccgca
      841 caaacccggc tcgatattgg taacgcggat gttcttatcc gccaactccg cgcgcaaatt
      901 caggctgaac tggcgcacaa acgccttggt tgccccgtaa acgttgctgc cggcataagc
      961 ataattaccc gcaatcgaac ccaaattcat cacatagccg ccgccgcgtt ccaccatttg
     1021 cggcaaaatc ttgcgcgtca gaaacgtcaa acccaaaaca ttggtttgaa tcattgtttc
     1081 ccaatctcca aaatccgcct tgtccgccgt gtccaaaccc aaagccagcc cggcattgtt
     1141 gatgaggcag tcgatgtcgg aaaattcatc ggggatgccg tttaaggcat tttccaccga
     1201 ttcgcggcgc gacacgtcca tttccaaagg gtaaaacaaa gcacccaatt catccgccaa
     1261 ggcctgaagc ctgtccgcac ggcgcgccgc accgataacg cggtatcccg ccccgacaaa
     1321 cgcacggcac atcgcttcgc cgaatcctgc cgaagcaccg gtaattaaaa cagccattgt
     1381 ttttcctttc tttctcgttt cccaaacctg tttcaggtat cataacaccc ttcagacggc
     1441 atcgcgccgg ctgaaaatga caacaaccat accaccacga caggacacat tcaatatgaa
     1501 aacccaaatc agccttgctg ctgccgccat cacactgctc ctttccgcct gcggaggcag
     1561 cggaatacct tcccaaccca aaggcgaaat ttccgaaaac cgcaccgccg cgttcaaatc
     1621 catgatgccc gaattcacac gtatgggcaa aatggtcaaa ggcgaagaac cttacgatgt
     1681 cgaaaaattc aaacaggcgg cagcgtcgtt tgccgaaagc agcaagaaac cgttcacact
     1741 ttttgagtcc gatccgcaag gcaacgggcg cgcactgcca gccgtttggt cggatggtgc
     1801 aaaatttgaa gccgaaaaaa caaaattcgc cgccgccgtc gaaaaactca acgccgccgc
     1861 ccaaaccggc aaactggatg aaatcaaagc cgcctacggc gaaaccggcg caagctgcaa
     1921 atcctgccac gacagtttcc gcgcgccgga ataacatccg aaacagaacg atgccgtctg
     1981 aagccttcag acggcatttt ctttgtcatt tcagcacacg caaagcaatc agaaattatc
     2041 ttcaattcaa aaaatacgcc atatcctgat ttttgcctta cttactccgc cattttctat
     2101 tttgatttgc tatgcgatta atctttatcg tcaaatgtca aaatcgggat gccggattgg
     2161 gtaaacaggg catacaccgg cttgagccaa taatcttccg gaagggaagc cggtttcaat
     2221 gcgtaaacgt gcacattggg aaaatccttc atcgtcaacg ccgcgccgct gaaaaaggta
     2281 taaatttcat acctcgtatg cgggtttttc ttaatctcgc gcaaaatata gtcttcgatg
     2341 acatagggcg aatttaatgt ggttacgccg gaaagcccgt aggtttggcg ggggtgcggt
     2401 gcgacatatt gtatgttgaa gttttttgcc gccttttccg aaatttcctt catctccttg
     2461 tcgggcgaac ccaaaagtat ccgcaccgtg ccgcccgttt cgtcccccgc cttcagttcg
     2521 gacgcatcga acagcggcag gtaagtcatc ttgcggcggc cgtcgtccat aatgtttttc
     2581 aaacccttga atatcgtgta atgctcgtcg gaagcattac gggttttggc gatgctccaa
     2641 tctccgatca tcatccgggc aaaattccgc ttgatcgtcc cgtttacaga aaactcatcg
     2701 cccaaatagc tgctgctttg aattaaattg cctgtcccgt cgtcaaaggt tttgatttcc
     2761 gcatccgggt aagtgctcaa aaaggcggca atgctgactt tttccaaact tgccaaataa
     2821 atccgtttga ccttcggcag cagcatcgac tttaccttca gctccgccat cgtcggaatg
     2881 aaattaaacg atttgttcaa accgtagggc aggtggaaaa agtacgcccg ctccgcttta
     2941 tcctttatct gcttgaaata ataatcgtat ttttcattcc tgttttcaga catcagcacc
     3001 acataaaacc gctcccccgg atgctgcgcc ataatccttt ccgccacctt catctgcaat
     3061 atggtatagc agaaaatcag attgaccggt tccccctctt cattgaagag tttgtccttc
     3121 agcagggaaa ccgcattcct ttccccatga tttacccggt caaatgtata aaatatcccg
     3181 aaacaaaaaa caatcaaaca caacacggtc aaacaagcct ttttcaagcc catatcccta
     3241 aaactccatt ccgacaaatt gaacatacgc cccgagtgtc ccgggacgga aaaatgcgga
     3301 attttagcag atattccgac agttaccgaa tattatagtg gtttaaattt aaatcaggac
     3361 aaggcgacga agccgcagac agtacaagca gtacgacaag gcaaggcaac gctgtactgg
     3421 tttaaattta aaccactata aaatcaattt caccgaaaat atgccgccgc acatcaggca
     3481 cagcacctcc gaccgccacc aaacgcggga aaaccatttc ccccttcctt tcacgctgcc
     3541 gcgttcaaac acgccaagat cgaacaaagc gcgaacaaag ccgccaaaat atgccataat
     3601 agccgcgcac attccccgcg caatgtgttc caaaagaaaa aaccaaccta caagagaata
     3661 atcctatgac tcaaaaatcc accattgttt atacccatac cgacgaagcc cccgcgctgg
     3721 cgacccaatc gctgctgccg attgtgcagg cttttgcccg ccacgccgat attgatgtca
     3781 aaactgccga catttccctg tccggccgta ttttggcggc attccccgaa tatctgaccg
     3841 aagcgcaacg cgtacccgac gcgcttgccg aattgggcga actggtaaaa caacccgatg
     3901 caaacgtaat caaactgccg aacatcagcg catccgtacc gcaactgacc gccgcgatta
     3961 aagaattgca gtctaaaggc tttgccgttc ccgattatcc tgccgacccg caaaccgatg
     4021 aagaaaaagc cgtgcgcgaa cgctacgacc gcatcaaagg cagcgcggta aaccctgtcc
     4081 tgcgtgaagg caactccgac cgtcgcgccc ccaaagcggt gaaaaacttt gccaaaaaaa
     4141 atccgcacag catgggcgca tggacaaaag actccaaaac ccacgttgcc accatgcaaa
     4201 gcggcgactt tttccataac gaacaatccg ttaccgtacc tgaagcgact tccgtatcca
     4261 tcgtgttcac cgacaaacaa ggcaataaaa aagagctgcg cgcgcctgtt gccctgaaag
     4321 ccggcgaaat catcgacgca accgtgatga gcaaaaaagc cctgctcgcc ttccttgccg
     4381 aacaagtgaa agacgcgaag gcaaaaggcg tattgttctc actgcatatg aaagccacga
     4441 tgatgaaagt gtccgacccg attatcttcg gacacgccgt caaagtgttc ttcgcgcctg
     4501 tatttgaaaa attcggcggc aaactggctg ccgcaggtgt caacgtcaac aacggcttcg
     4561 gcaacctgct ggccaatctg gacaaactgg atgcggacac ccgcgccgcc gtcgaagctg
     4621 aaatcgccgc cgtttacgcc gccaaccctg atttggcgat ggtcgattcc gataaaggca
     4681 ttaccaacct gcacgttccc agcgacgtca tcgtcgatgc ctctatgcct gcgatgattc
     4741 gcaattccgg ccggatgtgg gacaaagacg gcaaagcgca agacaccaaa gccgtcattc
     4801 ccgacagcag ctatgccggt gtttaccaag caaccatcga tttctgccgc gaacacggcg
     4861 cattcgatcc gacaactatg ggtaccgtgc ccaacgtcgg tctgatggcg caggcggccg
     4921 aagaatacgg ttcgcacaat aaaacttttg aaatcgaagc cgacggtcaa gtccaagtca
     4981 ttgatgcggc aggaaaagtc ctaatgcagc acgacgttga agccggcgac atctggcgta
     5041 tgtgccaaac caaagacgct ccggttaaag actgggtaca acttgccgtc aaccgcgccc
     5101 gtctgagcaa cacgcctgcc gtgttctggc tcgacgaaaa ccgtccgcac gacaagagcc
     5161 tgctcgccaa ggttaaagct taccttgccg aactggatac cgacggcctc gacatccgcg
     5221 tcctcgcgcc tgaagaagcc gccaagttca gcttgggccg tctgaaaaac ggcgaagaca
     5281 ccatctccgt aaccggtaat gtcttgcgcg actacctgac cgacctgttc ccgattttgg
     5341 aactcggcac aagcgcgaaa atgctgtcta tcgttccatt gatgaatggc ggcggtatgt
     5401 ttgaaaccgg tgcgggcggt tctgcaccga aacatgttca acaattcctc gaagaaaacc
     5461 atttgcgctg ggactcgctg ggcgaattcc tcgcactcgc cgtatcgttt gaacatttgg
     5521 cgcaaaaaac cggcaatgcc aaagcccaag tcctcgccga cactttggat gcagccaccg
     5581 aaaaactgct gttgaacgac aaatcgccta aacgtaaagc cggcgaactc gacaaccgcg
     5641 gcagccattt ctacctcacc ctctactggg cgcaagaatt ggcggcgcaa gacaaagatg
     5701 ccgaactgaa agccgcattt acgccattgg cagccgcttt gaccgccgac gaagcgaaaa
     5761 tcgttgccga gctttccgcc gtacaaggca aagcggccga catcggcggc tactacgccg
     5821 ccaatcctga aaaagccgca caagtgatgc gtccgagcgt aacctttaat caggcactaa
     5881 acgccttata agcacaaagg taaaaatgcc gtctgaacat tcgtcgttca gacggcatta
     5941 gttatcccta tctgcctgat tataattaaa cgccgcaccc attggatat
//
LOCUS       DAWFAN010000119         5972 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWFAN010000119 DAWFAN010000000
VERSION     DAWFAN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.119"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(41..964)
                     /gene="truB"
                     /locus_tag="WGO65_001554"
     CDS             complement(41..964)
                     /gene="truB"
                     /locus_tag="WGO65_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ0327834.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYAIDI
                     AEFDSPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLDETERDSLLLPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFDEDLTSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAVSAA"
     gene            complement(997..1368)
                     /gene="rbfA"
                     /locus_tag="WGO65_001555"
     CDS             complement(997..1368)
                     /gene="rbfA"
                     /locus_tag="WGO65_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ0327835.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            1559..2803
                     /gene="clpX"
                     /locus_tag="WGO65_001556"
     CDS             1559..2803
                     /gene="clpX"
                     /locus_tag="WGO65_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ0327836.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIAWQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGKEPELVFES"
     gene            complement(2868..4061)
                     /locus_tag="WGO65_001557"
     CDS             complement(2868..4061)
                     /locus_tag="WGO65_001557"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinyldiaminopimelate
                     transaminase"
                     /protein_id="HEZ0327837.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATETIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVCSQGQKLQTALRSLGEQTGVFKQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            4465..4965
                     /locus_tag="WGO65_001558"
     CDS             4465..4965
                     /locus_tag="WGO65_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327838.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHPLPL"
     gene            5050..5604
                     /locus_tag="WGO65_001559"
     CDS             5050..5604
                     /locus_tag="WGO65_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327839.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            5618..5818
                     /locus_tag="WGO65_001560"
     CDS             5618..5818
                     /locus_tag="WGO65_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327840.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
BASE COUNT         1415 a         1605 c         1624 g         1328 t
ORIGIN      
        1 aacacattat tcaaacacag cccgtatttt taaccgccgt tcaggcggca gataccgccg
       61 tgttcatcag gcgcaaggct ttcagacggc atatttcttt ttgatattcc gccaggccga
      121 caaagcggcc gttttccgtg taaacgcgta ccggcgtatc ggaagtcagg tcttcatcga
      181 aacgcggccg ttgcccgcat tggagcatat ggacggcata atcgtttaaa acggtttggg
      241 gaaaatgggc aaccaatacg tcgcagggca gcagcaggct gtcgcgttct gtttcgtcca
      301 agtttgccaa ggcttcgagc gtgtggcttt gggcgatggt aaagccggcg gtttcggtgc
      361 ggcgcagggc ggtcaggtgg gcgaacgtgc cgatgtgttt ggcgatgtct tcgctgaggg
      421 tgcggatata ggtgcctttg ctgcaacgca cgtcgatgac ggctttgggt gagtcaaact
      481 cggcaatatc gatggcgtaa acggtaatat cgcgcggctt gcgttcgatg acgatgcctt
      541 tgcgggcgta ttcgtacagc ggtttgcctt cgtgcttgag ggcggaaaac atcggcggca
      601 cttggcggat gttgcctgtc agggcttggc aggccgtctg aaattcggct aaggaaatat
      661 cggcgcgtgc ggcagcgatg atttcgcctt cggcatcacc cgtgctgctg gcttcgccga
      721 gtttcagcgt ggcggtgtag gctttgtcgg catccagcag gtattgggcg aacttggtcg
      781 cttcgccgaa gcagacgggc aaaagcccgg ttgccaaagg gtcgagcacg ccggtatgtc
      841 cggctttttc ggcacggaac aaacgccgcg ctttttgcag cgcggtgttg ctggaaaggc
      901 cttcgggttt gtccaagagc aaaacaccgt tgacggcacg tttttggggt ttgcctgtat
      961 tcatggttcg atgttcggac ggcatgggca tatccgtcag tcttcaaccg gtttttccgc
     1021 cgctacttgg tcgataaggg cggacaggtt caaaccgcgt tccaaggatt cgtcgtattt
     1081 gaaatgcagt tcggggattt tgaacagctt gatgcgtttg gccaattcgc tgcggaggtg
     1141 tccgcgcgcg tgttccagca cttcttccgt aatttcgcgc gcgtcttggt ttaaaatggt
     1201 gtagaacacg gtggcgtggc tgtaatcgcg ggtaacttcg acttcgttga cggtaatgaa
     1261 gccggcgcgc gggtctttca gtccggtacg gacgagttcg gcaagctcgc gcataatttg
     1321 ttctttgaca cggtcttggc gggcatagcc gcgttggggt tttctcatga atatcctcag
     1381 atttcagatt tggtttcaga cggcattttt gccgccgggg cggttgcgaa agggcgtatt
     1441 atagcctaat cggcgggatt ttaaggtaat acattgataa gaacggatat agttattata
     1501 cttggcaacc cgaacggcac gtgccgtttt ctgtaacgca tacattgtaa agaaagccat
     1561 gtccaacgaa aaccgtacct gttccttttg cggaaaatcc aaatcacacg tcaaacattt
     1621 aattgagggc gaaaacgcct ttatctgtga cgaatgcgtc tcaaactgta ttgaaatatt
     1681 gcatgaagac ggaaatgacg gcacgccttc ggaaagtgcc ggaggggagc cggaagaatc
     1741 cggcaagctg cccacgcccg ccgaaatcgt cgccaacctc aacgaccacg ttatcggtca
     1801 ggaacaagcc aagaaagcct tggcggtgtc ggtgtacaac cattacaaac gcctgcgcca
     1861 cccgaaagcc ggagctaatg tcgaattgtc gaaatccaac atcctgctta tcggcccgac
     1921 cggatcgggt aaaaccctgc tggcgcaatc tttggcgcgc aaactggatg tgccgttcgt
     1981 gatggcagat gcaaccacgc tgaccgaagc aggttatgtc ggcgaagatg tcgaacagat
     2041 tattaccaaa ctgttgggca aatgtgattt cgatgtcgaa aaagcccaac gcggcatcgt
     2101 ctatatcgac gaaatcgata aaatttcacg caaaagcgac aatccgtcca tcacgcgtga
     2161 cgtgtccggc gaaggcgtgc aacaagcctt gctgaaactg attgaaggta cggtggccag
     2221 cgttccgccc caaggcggcc gcaagcatcc gaatcaggaa tttatcaacg tcgataccac
     2281 caacatcctg tttatctgcg gcggcgcgtt tgcaggcttg gaaaaagtca tccgccagcg
     2341 tactgaaaaa ggcggtatcg gtttcggtgc gtccgttcac agcaaggacg aaaatgccga
     2401 cattaccaag ctgttcggca tcgtcgaacc ggaagattta atcaaattcg gtctgattcc
     2461 ggaattaatc ggacgtttgc ctgtgattgc aacgttagaa gaactggatg aggacgcgct
     2521 gattaatatt ttgaccgagc cgaaaaacgc tttggtcaag cagtatcaag ccttgttcgg
     2581 tatggaaaat gtcgagttgg aatttgaaga aggcgcattg cgttccattg cgtggcaggc
     2641 aatggagcgc aaaaccggcg cgcgcggcct gcgttccatc gtcgaacgct gcctgctcga
     2701 tacgatgtac cgcctgcctg atttgcaagg cttgaaaaaa gtggtggtcg gtaaggcagt
     2761 catcgaagag ggcaaggaac cggaattggt gttcgagtcc tgatggcaaa caaaaccgct
     2821 tcggaacgtt gaaccacgtt tccgaagcgg ttttttgttt ttgatgccta ggggcggttt
     2881 gcggcggcaa attccgtcag cacgtgttcc aaacgccgca aaccttccgc cgtatcctca
     2941 tcgtccaaca gcagcgaagg cgcgaaacgc aatacgtccg cacccgcaac caaaatcatc
     3001 acgccgtgtt tcaaggcggc ggcggttatt tcggatgcgc gtccgcgata aggcgcgtcc
     3061 aacacgcagc cgagcagcag ccccatgccg cgaacttgct taaacacgcc cgtttgttct
     3121 cccaaagacc gcaatgccgt ctgaagtttc tgcccctgac tgcagacgtt gtgcaaggtt
     3181 tccggcgcgt tgatgatgtc gaaggcgcgg ctgccgaccg cacacgccat cgggttgccg
     3241 ccgaaagtcg agccatgcgt gcccggttgg aaggcggcgg caatcgtttc tgtcgccagc
     3301 atcgcgccga tcggaaagcc gcagcccaag gcttttgccg aactcaaaat atcgggaaca
     3361 acgccgtaat gttcataggc aaacagcctg cccgtatgcc ccatcccggt ttgaacttcg
     3421 tccaaaatca acaacgcatt gtgccggtcg cacagacggc gcgcggtttg caaatattcc
     3481 gcagtggcgg gcaggatgcc gctttcgccc tgtatcggct cgatgatgac cgcgcaggtc
     3541 tgttcgccga cggcagcttc cagcgcggca atatcgttga acggaacgtg cgtaatgcct
     3601 tgcggcaggg gtgcataatc cttgctgtat ttcggctgac cgccgacgga cacggtaaac
     3661 agcgtgcgtc cgtgaaaact gttgatacag gcgacgattt cgctttttcc tccgccgaaa
     3721 cggtcgcggg cgtatttcct cgccagcttc aacgccgcct cattcgcttc cgagcccgag
     3781 ttgcagaaaa aaaccttgtc ggcaaaactg tttgcaacca atttttgcgc caattcctgc
     3841 gctggacgcg tcgtatagat attggaaatg tgccacagct tgtgcatctg cgcgtttaaa
     3901 gcatcgacaa gggcagggtg gcagtgtccc agcgcattga cggcgatacc gcctgaaaaa
     3961 tcaatatatt cacgcccttt cgtatcccaa acgcggctgc ctgaagcgcg ttcgggaatc
     4021 atcggggcaa aggcgaaatt gggggtcaga tagttttgca taattgctcc tgattgcggt
     4081 tttgctcggg attatgcacc gtttttcaaa gcagataaag ggaaattgcc gacaatatcg
     4141 ggcagcggta aaaataaacc gaaatatttt caatcggata gatgaaaaat caaaaaaatt
     4201 tcgataaaat gcttgaggcg cggcagcgtt ttgatttaga atctccccaa tatttttatc
     4261 ttcagacggc atttcatcat gcaaggcaaa aaatttacaa ccgcttattg ctattggtac
     4321 cacacagacg tgcggtactt ttttgcgctt gcttgaaatc catcccatac gcaaaacagg
     4381 ccgctcgaaa acggggcggc ttttttgtta ccttcacaca ggtcgcccga ttcggcaaca
     4441 cacgacagaa tcaggaaaat gaccatgaat gcaatgcccc aaaccgccgc acaaaacgaa
     4501 accctgcaca cttgtagcat tattgtcgat gatgcacagg atttttcccc catgcttctc
     4561 gatgcagggc gggacgacac acttatcagc gaatcgatga ttccgcaaat ccgcaccgtt
     4621 gccgccctga tagccgccga acggcacgac ttcagccgca gcagccccgc cgagtttacc
     4681 gatgccgccg atttttttgc cgcacggatt ctcgtattgg gcgtgcgacg cttccatctg
     4741 gacgtatcgc tgcttcagat tttgaaaacc gccaacagcc gcgcccgccg ctttgccgaa
     4801 aaacaccgcc tgtttttcac acccgcacaa gccgaattga gcctgaacaa acacaaaaac
     4861 cgccgcctgc tgaccgtaga aaccgaacac gaagtcgaaa acaaaggcaa cccagtcgcc
     4921 aacagcctcg catttgtccg caaattgcac cccctgcccc tgtaaacatc acgatttgcc
     4981 ggcagaatgc ttgccaaaat tcccgacggc gatacaataa aaccgatttt ccacccaaac
     5041 aggacacaca tgaaaaaaat catcgcctcc gcgcttatcg caacattcgc actcgccgcc
     5101 tgccaagacg acacgcaggc gcggctcgaa cagcagcaga aacagattga agccctgcaa
     5161 cagcagctcg cacagcaggc agacgatacg gtttaccaac tgactcccga agcagtcaaa
     5221 gacaccattc ctgccgaagc acaggcaaac ggcaacaacg ggcaacccgt taccggtaaa
     5281 gacgggcagc agtatattta cgaccaatcg acaggaagct ggctgctgca aagcctggtc
     5341 ggcgcggcgg caggcgcgtt tatcggcaac gcgctggcaa acaaattcac acgggcaggc
     5401 aaccaagaca gtcccgtcgc ccggcgcgcg cgtgcagcct accatcagtc cgcacgcccc
     5461 aatgcgcgca ccagcaggga tttgaacacg cgcagcctcc gtgcaaaaca acaggcggcg
     5521 caggcgcagc gttaccgccc gacaacgcgc ccgcccgcca attaccgccg ccccgctatg
     5581 cgcggtttcg gcaggaggcg gtaaacccgg cgcaccaatg ccgtctgaag agctttcaga
     5641 cggcattttt gcatttgtta gggacattgt tatgttgccg tttgattttc agacggcatt
     5701 ttgtttccaa gcgtttgatg tcgggatggc aattctgatt tacgtgcgtt ggcagggcgg
     5761 cattgtcatg ccgccgttgg gttttcagac ggcatcatat cgaacagtca agccgtaggg
     5821 cgagcattct tgcccaccgt tgcaagggcg ggatttggtg ggcaggaatg cccaccctgc
     5881 atctgaaacc cttccgtgtc attcccgtgc aggaaggaat ctagatattt gaatttacgg
     5941 aaattttcaa tcgttgccaa agcggaaaag gg
//
LOCUS       DAWFAN010000120         5903 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWFAN010000120 DAWFAN010000000
VERSION     DAWFAN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.120"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            124..2142
                     /gene="uvrB"
                     /locus_tag="WGO65_001561"
     CDS             124..2142
                     /gene="uvrB"
                     /locus_tag="WGO65_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ0327841.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACDSIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPSNTIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDGGKGRLKGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRGIKENLLF"
     gene            2153..2944
                     /locus_tag="WGO65_001562"
     CDS             2153..2944
                     /locus_tag="WGO65_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327842.1"
                     /translation="MIKMKDIYESICCKSIRKIMYISDSFVAISGTGFIIKFQEKFYF
                     CCLYHCMSNEGKFEKNKLFGLSILYKTYLEIDPKTISESNVANYVLFPNPEQITKEFC
                     SSKIDDDLMLDEEFIPIEIIGENELIPCGYLDFDIEYWSNYLFSEKKPIENEILLVCG
                     FPFSDNEIDYTDDGEIKKVSHQVHIYAGKCKKDDIGFFVEKEDKNVSFDCNGVSGGIV
                     MRMIDGHSEWVGIAVRANNDIIRFIPYYLVAKSIIQNILNRNQQA"
     gene            3063..3134
                     /locus_tag="WGO65_001563"
                     /pseudo
     CDS             3063..3134
                     /locus_tag="WGO65_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3609..4325)
                     /gene="trmB"
                     /locus_tag="WGO65_001564"
     CDS             complement(3609..4325)
                     /gene="trmB"
                     /locus_tag="WGO65_001564"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ0327843.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(4447..5850)
                     /locus_tag="WGO65_001565"
     CDS             complement(4447..5850)
                     /locus_tag="WGO65_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0327844.1"
                     /translation="MNNPDLPYRQALECLSQKQYNFTEVRRLLTEAFSAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKAAAQGLAAAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDIYRYGLGMTSDKEKALHYYRQAAEAGSLAAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
BASE COUNT         1430 a         1458 c         1497 g         1518 t
ORIGIN      
        1 ttgggtagcg attgggtttc agacggcata cattcaggat ttcaccgcct tatgccgtct
       61 gaaagacgtt tccgacccat ccctactaca cgctttgtcc gcaaacgcat cacaggcaag
      121 attatggaag tcattcgata ccccgattcc cccttcaaac tccaccaacc cttcccgccc
      181 gccggcgacc agcccaccgc cattgccggc ctgctcgaag ggctttcaga cggccttgcc
      241 tatcaaaccc tgctcggcgt aaccggttcg ggcaaaacct acaccatggc gaacgtcatc
      301 gcccaaagcg gccgacccgc catcatcatg gcgcacaaca aaacccttgc cgcccagctc
      361 tatgccgaaa tgcgcgagtt tttccccgaa aacgcggtgg aatatttcgt ctcctactac
      421 gactattacc agcccgaagc ctatgtgccc agccgcgact tgttcatcga aaaagacagc
      481 gcgatcaatg aacacatcga acaaatgcgc ctttccgcca ccaaaaacct gatgacgcgc
      541 gacgacgtga ttatcgtcgc caccgtgtcc gccatttacg gtatcggcga cccgactgag
      601 tatcaacaaa tggtgttgtc cgtcaaagaa ggcgacacca tcgagcagcg cgacatcatc
      661 gccacgctcg tttccatgca gtacgaacgc ggcgatttgg acttcaaacg cggcagcttc
      721 cgcgtgcgcg gcgacgtgat tgacgtgtac cccgccgaaa gctccgaaaa cgccttgcgt
      781 atcagcctgt tcgacgacga aatcgaccgc ctcgatatgt tcgacccgct ttcaggcagc
      841 cttatccaac gcgtcggccg ctacaccgtc ttcccgtcca gccactacgt taccccgcgc
      901 gacaccgtat tgcgcgcctg tgactccatc aaagaagaat tgcgcgaacg cattgatttc
      961 tttaccaaag aaaaccgtcc cgtcgaacaa caacgcatcg aacagcgcac ccgtttcgac
     1021 ctcgaaatgc tctacgaaat gggcttctgt aaaggaatcg aaaactactc ccgccacttc
     1081 tccggcaaaa aagaaggcga accgccgccc acgctgatgg actacctgcc aagcaacacc
     1141 atcatgttca tcgacgaaag ccacgttacc gttacccaaa tcggcggcat gtacaaaggc
     1201 gacgcctcgc gcaaacaaaa cctcgtagat tacggcttcc gcctgccttc cgcccgcgac
     1261 aaccgcccgc tcaaattcca cgaatttgaa aaagtcatgc cgcaaaccgt cttcgtttcc
     1321 gccactcccg ccaaatacga agaagaacac gccggacaag tggtcgaaca agtcgtccgt
     1381 cccacagggc tggtcgaccc ccaaatcatc atccgccccg tcgccaccca agttgatgac
     1441 ttaatgagcg aaatcaacga ccgtattcaa aaaggcgaac gtgtgctcgt taccaccctc
     1501 accaaacgca tggcggagca actcaccgac tattacagcg aactcggcat caaagtacgc
     1561 tacctgcaca gcgacatcga caccgtagag cgcgttgaaa tcattagaga tttgcggctc
     1621 ggcctgtttg acgtactcgt cggcatcaac ctcttgcgcg aaggcctcga catccccgaa
     1681 gtctccctcg tcgccatcct cgacgccgac aaagaaggct tcctgcgctc ccaccgcagc
     1741 ctgattcaaa ccataggccg cgccgcacgc aacgtgaacg gcgtcgccat cctgtacgcc
     1801 gataaaatca ccgactccat gaaagccgcc atcgacgaaa ccgaacgccg ccgcgaaaaa
     1861 cagattaaat tcaacgaaga acacggcatc gtaccgcagc agattaaaaa acaggtaaaa
     1921 gacattatcg acggcgtgta ccacgaagaa gacggcggaa aaggccgtct gaaaggtaaa
     1981 aacaaggtta aagtgggcga gattcacaac gaagaagatg cgattaaaga aatcgccaaa
     2041 ctcgaaaaag ccatgcagca ggcggctagg gatttgcagt ttgaagaggc ggctgtgttg
     2101 cgggatagga ttcgggggat taaggagaat ttattatttt aggatgtaaa aaatgattaa
     2161 gatgaaagat atttatgagt caatttgttg taaatctatt agaaagataa tgtatatatc
     2221 agatagtttt gtagctattt caggaacggg gtttattatt aaatttcagg aaaaatttta
     2281 tttttgctgt ttatatcatt gcatgtctaa tgaaggaaaa tttgaaaaaa ataaattgtt
     2341 tgggttaagt attttatata aaacatatct tgaaatagat cccaaaacta tatctgaatc
     2401 taatgtagct aattatgtac tttttcctaa tcctgagcaa attacaaaag agttttgtag
     2461 ttcaaaaatt gatgatgatt taatgcttga tgaggaattt atccctattg agattattgg
     2521 agaaaatgaa cttataccat gtgggtattt ggattttgat atcgaatatt ggagtaatta
     2581 tttgttttca gaaaaaaaac caatagaaaa tgaaattctt ttagtatgcg ggttcccttt
     2641 ttcagataat gaaatagatt atactgatga tggtgagatt aaaaaagtat cgcatcaagt
     2701 gcatatatat gctggtaaat gtaaaaaaga cgatataggc ttttttgttg aaaaagaaga
     2761 taaaaatgtt tcatttgatt gtaatggtgt tagtggtgga attgtgatga gaatgataga
     2821 tggacacagt gagtgggttg gaattgctgt tagggcaaat aatgacatca ttcgttttat
     2881 tccttattat ctcgtagcga aatcaataat ccagaatatc ttaaatagaa atcaacaagc
     2941 gtaggtcgga tacttgtatc cgacaaaacc ttcaacattc ccgtcgttgc aatccattgc
     3001 agctatgtcc gaaatgtcgg attcgaaaat ccgacctaca aaatcattcc gagcataaca
     3061 ctatgaaata acgtcgtttt taccgcaatg acggcactta cttttttacg gttgcaacca
     3121 ataaacggca gcaagcgtag ggtgggcact tgttgcccac gcgtttatct ccgaaaggca
     3181 gcaactgtag gtcggatact tgtatccaac aaacctttaa cattctcgtt gttgcaatcc
     3241 attgcagcaa tgcccaaaat gtcggattcg agaatccgac ctacaaaatc attccgaaca
     3301 taatactatg aaataccgtc gttttaccgc aatggcggca cttacttttt tacggttgta
     3361 accagcaagc cgtagcctac atcacaccaa ccgcgtgcgt agctatcaca tactacctgc
     3421 gggcggcaca aactttaaaa ccgatgtgca ggttaaaacc tacaaggatg cgtgcggtac
     3481 acacgcgttt tgctgttcgc accattgctt tgaactttat atcgaaacat cactcgctgc
     3541 tttatcttga aaaaatgccg tttgtaagca aacatgcttc agacggcatt tttttatccg
     3601 gcggtttgtt atccgatccg tttgaatacc aagtcccaaa cgccgtgtcc gaggcgtttg
     3661 ccgcgcgctt cgaatttggt ttcggggcgg tagtccgggg tgggggcgta gtctgccgcc
     3721 gtattttgca ggctgtcgaa gctgctgagg acttcaagca tctgctgtgc atattcttcc
     3781 cagtctgtcg ccaggtggat atagccgccg gttttgagtt tgggcagcag tttggcgatg
     3841 aagggggctt gtatcagacg gcgtttgtgg tggcgttttt tgtgccacgg gtcggggaag
     3901 aatatgtgga tgccgtcgag cgagccgtct tgcagcatat tttcgacaac ttctacggca
     3961 tcgtgccgca tcacgcggat attttccaaa tggttttcgt ctataagttt gagcagatta
     4021 cccacgccgg gaccgtgtac gtcgatggcg agaaagtcgg tttcgggcag cctgcgggcg
     4081 atttctgcgg ttgctgtccc cataccgaag cctatttcga ggattttggg ccggttgctt
     4141 ccgaaattgg catcaagatc ggccggtgtt gcttggtaat cgatgccgaa gctgtcccat
     4201 aaggtatcga tggcgcgttg ctgcgcggcg gtcatatggc tttggcggag gacgaagctg
     4261 cggatgctgc gggggtgtcc ggcttgacgg tcgtttggtg tttggttttc ggcggtgtct
     4321 gtcataatgc tttcatgcgc ttgtgcaatc aggatgccgt ctgaatgtga aatgtctgtt
     4381 cggacggcgg gaagggaaag gggcgattat acctgtttcg tttcggcagg gtaggtgtaa
     4441 cgctgttcag acggcatttt gccactgttg caacagttct tgcaggacgc ttttttgccc
     4501 gtatccgttg tttatggctt cttgcaggta gcggcaggct ttttctttgt ccggcccgac
     4561 accgtgtccg ctgtaatgga tgcaggcgag gttgtagaac gccatactgt ctttttttgc
     4621 ggcggctttt tcaaaccatt tgcgtgcttc attgtagtcg gcggtcatac cctgtccgaa
     4681 gtaatacatg ctgcccagat tggtttgggc tttgctgtgt ccgagttctg cggcttctgt
     4741 gtagagtttg agcgcggctg cgtattcggg cgcgcattgt aatccgtaat gcagggcttg
     4801 ggctttttga taaagccgtt ctgccctttg ccgcctgatg gcggaatctt taatgccgcc
     4861 atattggtcg ggatggttta acgcgctgtc ggatatgagt ttctgatacg cggcaaggct
     4921 gccggcttcg gctgcctgcc ggtaataatg cagggctttt tctttatcgg aagtcatgcc
     4981 cagtccgtag cggtagatgt cgcccagtat ccgcaaacct tcgggatggc gttcggcggc
     5041 ggctgcttcg gcgtgatgca gggcaagttt gtagtttctt tccggatgct tgccttgcag
     5101 gtagatgtcg gcaagtgcgg catgggcatc ggggtcattt tcctgtgccg cctgttgaaa
     5161 ccagtgaacg gcttcatcag gagccgtagg taggatgagc tcggcaagcg gggtgtaggc
     5221 gggaatgtat ccttgttgtg cggcggcgcg caaatgattg acggcctgtg ccgtgtcgac
     5281 tttcgtccct tgtccgaaac ggtattgcag cccgagctgc cagtgtgccg ccgccaagcc
     5341 ttgtgccgcc gctttgcggt agagcaggtg cgccgtttca aaatccggcg gcgtgccgta
     5401 ccggcagtat tgggcaagca ggtattgccc gtgggggttg ttgttttttg cgcttgtttt
     5461 tgcccatttc agggcttctt caaaatgtcg ggacgcatac agaaggtaca tcaggcggac
     5521 ttgtgcttca ggcagtcctt cctctgccaa cggtctgtaa agcggtatca gggtttccgg
     5581 gggcgcgcct tccaattctt ggatatatgc cagattgtaa cgcgcatagg gatgcccctg
     5641 ttcagcggcg atgcggagca tttccatacc ttgttcgcgg tcttggtagg ggctgtccgc
     5701 gtccatcagg tgttttgcca actcgaatgc ggcggcggga tgacctgccg agaacgcttc
     5761 tgtcagcagt cggcggactt cggtaaagtt atattgtttt tgagacaggc attctaaggc
     5821 ctgccgatag ggtaagtcgg gattgttcat gtcagatcgt tttccgtttt gtcgaatcat
     5881 cgttattgtg ttatgaagtt agg
//
LOCUS       DAWFAN010000121         5873 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWFAN010000121 DAWFAN010000000
VERSION     DAWFAN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.121"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..1383
                     /gene="autA"
                     /locus_tag="WGO65_001566"
     CDS             <1..1383
                     /gene="autA"
                     /locus_tag="WGO65_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoaggregation autotransporter AutA"
                     /protein_id="HEZ0327845.1"
                     /translation="KLHVKIENKSHVAGQVLELTKMTLKDSLWEPRRHSDIHMLETSD
                     NARIRLNTKDEKLTVHKAYQGGADFLFGYDVRESDKPALTFEEKVSGQSGVVLERRPE
                     NLKTLDGRKLIAAEKADSNSFAFKQNYRQGLYELLLKQCEGGFCLGVQRLAIPEAEAV
                     LYAQQAYAANTLFGLRAADRGDDVYAADPSRQKLWLRFIGGRSHQNIRGGAAADGRRK
                     GVQIGGEVFVRQNEGSRLAIGVMGGRAGQHASVNGKGGAAGSYLHGYGGGVYAAWHQL
                     RDKQTGAYLDGWLQYQRFKHRINDENRAERYKTKGWTASVEGGYNALVAEGVVGKGNN
                     VRFYLQPQAQFTYLGVNGGFTDSEGTAVGLLGSGQWQSRAGIRAKTRFALRNGVNLQP
                     FAAFNVLHRSKSFGVEMDGEKQTLAGRTALEGRFGIEAGWKGHMSARIGYGKRTDGDK
                     EAALSLKWLF"
     gene            1625..3091
                     /locus_tag="WGO65_001567"
     CDS             1625..3091
                     /locus_tag="WGO65_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0327846.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQTDGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFDNRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(3292..4260)
                     /locus_tag="WGO65_001568"
     CDS             complement(3292..4260)
                     /locus_tag="WGO65_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327847.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYASVLQQWEEHWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            4338..5630
                     /locus_tag="WGO65_001569"
     CDS             4338..5630
                     /locus_tag="WGO65_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ0327848.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
BASE COUNT         1309 a         1531 c         1820 g         1213 t
ORIGIN      
        1 aaattacacg tcaaaatcga aaacaaatcc cacgtcgccg gacaggtgtt ggaactgacc
       61 aagatgacgc tgaaagattc cctttgggaa ccgcgccgcc attccgacat ccatatgctg
      121 gaaacttccg ataatgcccg catccgcctg aacacgaaag atgaaaaact gaccgtccat
      181 aaagcgtatc agggcggtgc ggatttcctg ttcggctacg acgtgcggga gtcggacaaa
      241 cccgccctga cctttgaaga aaaagtcagc ggacaatccg gcgtggtttt ggaacgccgg
      301 ccggaaaatc tgaaaacgct cgacgggcgc aaactgattg cggcggaaaa ggcagactct
      361 aattcgtttg cgtttaaaca aaattaccgg cagggactgt acgaattatt gctcaagcaa
      421 tgcgaaggcg gattttgctt gggcgtgcag cgtttggcta tccccgaggc ggaagcggtt
      481 ttatatgccc aacaggctta tgcggcaaat actttgtttg ggctgcgtgc cgccgacagg
      541 ggcgacgacg tgtatgccgc cgatccgtcc cgtcaaaaat tgtggctgcg cttcatcggc
      601 ggtcggtcgc atcaaaatat acggggcggc gcggctgcgg acgggcggcg caaaggcgtg
      661 caaatcggcg gcgaggtgtt tgtacggcaa aatgaaggca gccgactggc aatcggcgtg
      721 atgggcggca gggccggcca gcacgcatca gtcaacggca aaggcggtgc ggcaggcagt
      781 tatttgcatg gttatggcgg gggtgtttat gctgcgtggc atcagttgcg cgataaacaa
      841 acgggtgcgt atttggacgg ctggttgcaa taccaacgtt tcaaacatcg catcaatgat
      901 gaaaaccgtg cggaacgcta caaaaccaaa ggttggacgg cttctgtcga aggcggctac
      961 aacgcgcttg tggcggaagg cgttgtcgga aaaggcaata atgtgcggtt ttacctgcaa
     1021 ccgcaggctc agtttaccta cttgggcgta aacggcggct ttaccgacag cgaggggacg
     1081 gcggtcggac tgctcggcag cggtcagtgg caaagccgcg ccggcattcg ggcaaaaacc
     1141 cgttttgctt tgcgtaatgg tgtcaatctt cagccttttg ccgcttttaa tgttttgcac
     1201 aggtcaaaat ctttcggcgt ggaaatggac ggcgaaaaac agacgctggc aggcaggacg
     1261 gcgctcgaag ggcggttcgg cattgaagcc ggttggaaag gccatatgtc cgcacgcatc
     1321 ggatacggca aaaggacgga cggcgacaaa gaagccgcat tgtcgctcaa atggctgttt
     1381 tgatgcgtcg ggaaatgttt tgacgcacag gcggcacacc ggcacggccc cgcgcgccgc
     1441 cccgcaaacc aatccgaacc ctgccgcccc gaagggcggg gcatcatgat gaaaccggcg
     1501 gaaaaccgcc ggttttttgc cgccgtttga aacccgattc tggcttcaga cggcattgtc
     1561 gcggcatcgg gcggcagggt ttggaacagc ggcataaaaa actgatacaa tccgccgatt
     1621 gataatggtt attttttatt tttgtgggaa gacatttatg cctgcacgaa acagatggat
     1681 gctgctgctg cctttattgg caagcgcggc atatgccgaa gaaacaccgc gcgaaccgga
     1741 tttgagaagc cgtcccgagt tcaggcttca tgaagcggag gtcaaaccaa tcgacaggga
     1801 gaaggtaccg gggcaggtgc gggaaaaagg aaaagttttg cagattgacg gcgaaaccct
     1861 gctgaaaaat cccgaattgc tgtcccgcgc gatgtattcc gcagtggtct caaacaatat
     1921 tgccggtatc cgcgttattt tgccgattta cctacaacag gcgcagcagg ataagatgtt
     1981 ggcactttat gcacaaggga ttttggcgca gacagacggt agggtgaaag aagcgatttc
     2041 ccattaccgg gaattgattg ccgcccaacc cgacgcgccc gccgtccgta tgcgtttggc
     2101 ggcggcattg tttgacaaca ggcagaacga ggcggcggca gaccagttcg accgcctgaa
     2161 ggcggaaaac ctgccgccgc agctgatgga gcaggtcgag ctgtaccgca aggcattgcg
     2221 cgaacgcgat gcgtggaagg taaatggcgg cttcagcgtc acccgcgaac acaatatcaa
     2281 ccaagccccg aaacggcagc agtacggcaa atggactttc ccgaaacagg tggacggcac
     2341 ggcggtcaat taccggctcg gcgcggagaa aaaatggtcg ctgaaaaacg gctggtacac
     2401 gacggcgggc ggcgacgtgt ccggcagggt ttatccgggg aataagaaat tcaacgatat
     2461 gacggcaggc gtttccggcg gcatcggttt tgccgaccgg cgcaaagatg ccgggctggc
     2521 agtgttccac gaacgccgca cctacggcaa cgacgcttat tcttacacca acggcgcacg
     2581 cctttatttc aaccgttggc aaaccccgaa atggcaaacg ttgtcttcgg cggagtgggg
     2641 gcgtttgaag aatacgcgcc gggcgcgttc cgacaatacc catttgcaaa tttccaattc
     2701 gctggtgttt taccggaatg cgcgccaata ttggatgggc ggtttggatt tttaccgcga
     2761 gcgcaacccc gccgaccggg gcgacaattt caaccgttac ggcctgcgct ttgcctgggg
     2821 gcaggaatgg ggcggcagcg gcctgtcttc gctgttgcgc ctcggcgcgg cgaaacggca
     2881 ttatgaaaaa cccggctttt tcagcggttt taaaggggaa aggcgcaggg ataaagaatt
     2941 gaacacatcc ttgagccttt ggcaccgggc attgcatttc aaaggcatca cgccgcgcct
     3001 gacgttgtcg caccgcgaaa cgcggagtaa cgatgtgttc aacgaatacg agaaaaatcg
     3061 ggcgtttgtc gagtttaata aaacgttctg attgctgttc cttttcggag gaaaccctgc
     3121 cggcggcggt atcacggcgg gcatcggcgg ctttcgggcg gtgctttgcg tgccgccgcg
     3181 tgtgcggaaa cgcattccgg tttttccggc ataacggcga tgcgaggtaa aatgccgtct
     3241 gaaacccgat tcgggcttca gacggcattg tcgcggttgc ggcgggcggg ttcaccagat
     3301 tccgtcaaag gttttcgcgc cgcgccaaaa tttccacctg tcggcgggtt tgaaggtcag
     3361 cgtaccgccg tgttgtccgt ccgtggtgat gtccagccgt ttgattttgc cggtacggac
     3421 ggcttcgtag atcggtgcga accagtgttc ttcccactgc tgtaaaaccg atgcataccg
     3481 ctccctgtcc cctgtcaggg cggtcaggcg caaatcgtcc ataaacagga tatggtgcgt
     3541 gtcgggcagg tgtgccgccg tttcttcata ggcgcggaag ttgtcgggca atgcgcggcg
     3601 gtcggggtgg aaacggctcc aaaccgtatc ggcgaaaagc gtgccgcctt gactgcttcc
     3661 gtgcagactg tcccaaagcc ataagccgtt caactcgggc agcccgcgtt tcttgcggtt
     3721 atggttgacg gggtgcgccg ccagccacat ttggatttcg gtttggacgc gcagccattc
     3781 caacgcgtct tccccgtccg gttgatcgtc cgcacccaac agtccgccca agtccaaaac
     3841 gggcttcgcg ccccagcggt acgcgcaagg aagggaaacc agccataatt cgggcaggac
     3901 gggaacgaaa cgccatggaa tgtcgccgta aaacgccgac aggtcgcggc agagccgttc
     3961 cgcttcatcc gtaccgacgt tcagatattc cgccgttagc acatttgcct gatgcatccc
     4021 catcttttgc cagacgggcg tggcgagcgc gacggcttca gacggcatat tcaggctttg
     4081 tgccgcgcgt tccgccagtc tgccgcacca caaataacgc gcgtaaaatg ccgaagccgt
     4141 gcagctttgg cggtgcagcg agccgtattg caggattttg ttgaaggcgg gcaggcagag
     4201 aggtattcgg gtttcgtctt catccaaatt gagcgaggga atggcgaggg tgagtttcat
     4261 cgtttgacgt ttcagaaatg caggtcaggc gcaacattat agaggattcg gcgcaaacgc
     4321 cgtcaaaaag gaacaatatg gctgtcttcc cactttcggc aaaacatcgg aaatacgcgc
     4381 tgcgcgcgct tgccgtttcg attattttgg tgtcggcggc atacattgct tcgacagaga
     4441 ggacggagcg cgtcagaccg cagcgcgtgg aacaaaaact gccgccgctg tcttggggcg
     4501 gcagcggtgt tcagacggca tattgggtgc aggaggcggt gcagccaggc gactcgctgg
     4561 cggacgtgct ggcgcgttcg ggtatggcgc gggacgaaat tgcccgaata acggaaaaat
     4621 atggcggcga agccgatttg cggcatttgc gtgccgacca gtcggttcat gttttggtcg
     4681 gcggcgacgg cggcgcgcgc gaagtgcagt tttttaccga cgaagacggc gagcgcaatc
     4741 tggttgcttt ggaaaaaaaa ggcggcatat ggcggcggtc ggcttctgag gcggatatga
     4801 aggttttgcc gacgctgcgt tcggtcgtgg tcaaaacgtc ggcgcgcggt tcgctggcgc
     4861 gggcggaagt gcccgtcgaa atccgcgaat ccttaagcgg gattttcgcc ggccgcttca
     4921 gccttgacgg tttgaaggaa ggcgatgccg tgcgcctgat ttacgacagc ctgtatttcc
     4981 acgggcagca ggtggcggcg ggcgatattt tggcggctga agtcgttaag ggcggcacaa
     5041 ggcatcaggc gttctattac cgttcggaca aggaaggcgg agggggcggc aattattacg
     5101 atgaagacgg cagggtgttg caggaaaaag gcggcttcaa catcgagccg ctggtctata
     5161 cgcgcatttc ttcgccgttc ggctaccgca tgcaccccat cctgcacact tggcggctgc
     5221 acacgggcat cgattatgcc gcaccgcagg gaacgccggt cagggcttcc gccgacggcg
     5281 tgattacctt taaaggccgg aagggcggat acggcaacgc ggtgatgata cgccacgcca
     5341 acggtgtgga aacgctgtac gcgcacttga gcgcgttttc gcaggcacaa ggcaatgtgc
     5401 gcggcggcga ggtcatcggt tttgtcggtt cgacagggcg ttcgaccggg ccgcacctgc
     5461 attacgaggc acgcatcaac gggcagcccg tcaatcctgt ttcagtcgca ttgccgacac
     5521 ccgaattgac gcaggcggac aaggcggcgt ttgccgcgca gaaacagaag gcggacgcgc
     5581 tgcttgcgcg cttgcgcggc ataccggtta ccgtgtcgca atcggattga agtttgaacc
     5641 ggcgacgaaa acaatgccgt ctgaaaatct gcaaacaggt tttcagacgg catttatagt
     5701 ggattaacaa aaaccagtac gtcgttgcct cgccttagct caaagagaac gattctctaa
     5761 gatgctgaag caccaagtga atcggtttcg tactatttgt actgtctgcg gcttcgtcgc
     5821 tttgtcctga tttttgttaa tccaccatac agtcgattaa aataaaacta agt
//
LOCUS       DAWFAN010000122         5836 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWFAN010000122 DAWFAN010000000
VERSION     DAWFAN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.122"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            266..1213
                     /locus_tag="WGO65_001570"
     CDS             266..1213
                     /locus_tag="WGO65_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0327849.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAIAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFEHPKVVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAG
                     SMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLTIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1366..2490)
                     /gene="rluD"
                     /locus_tag="WGO65_001571"
     CDS             complement(1366..2490)
                     /gene="rluD"
                     /locus_tag="WGO65_001571"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ0327850.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIIYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2489..3292
                     /locus_tag="WGO65_001572"
     CDS             2489..3292
                     /locus_tag="WGO65_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ0327851.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3350..5569)
                     /locus_tag="WGO65_001573"
     CDS             complement(3350..5569)
                     /locus_tag="WGO65_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ0327852.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWILTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPSVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1421 a         1833 c         1406 g         1176 t
ORIGIN      
        1 tttcaacagg ggatggcaca tcaagcaccg cactgcgtgt tgcccgaacg agactaccct
       61 ccgattagat tctatcgcta taaacagacg gatttcaacc ggaaaggaat ggggataaag
      121 tccgtttccg acacctctcg ggcgataccg tctgaaaacc aatctccact ttcagacggc
      181 attgtttagg cacggtcatt ttgcaaatta aggttaaggc gtgtatgctt gccttccctt
      241 aaaaacaaac acgcaacgca ttattatgaa tatcctcagt aaaatcagca gctttatcgg
      301 aaaaacattt tccctctggg ccgcgctctt tgccgccatc gcttttttcg cgcccgacac
      361 cttcaaatgg gcggggcctt atattccttg gctgttgggc attattatgt tcggtatggg
      421 tttgacgctc aaaccttccg acttcgatat tttgttcgaa catcccaaag tcgtcatcat
      481 cggcgtaatc gcacaattcg ccattatgcc ggcaaccgcc tggctgctgt ccaaactgtt
      541 gaacctgcct gccgaaatcg cggtcggcgt gattttggtc ggctgctgcc cgggcggtac
      601 ggcttccaat gtgatgacct atctggcgcg cggcaatgtg gctttgtcgg ttgccgttac
      661 gtctgtttcc accctgattt ccccattgct gactcccgcc atcttcctga tgcttgccgg
      721 tgaaatgctg gaaatccaag cgggcagtat gttgatgtcc atcgtcaaaa tggttttgct
      781 ccccattgtt ttgggtttga ttgtccataa ggttttgggc agtaaaaccg aaaagctgac
      841 cgatgcgctg ccgctggttt ccgttgccgc catcgtgctg attatcggcg cggttgttgg
      901 ggcaagcaaa ggcaagatta tggaaagcgg cctgctgatt tttgcggttg tcgtactcca
      961 caacggcatc ggctacctgc tcggcttctt tgccgccaaa tggaccggcc tgccttatga
     1021 tgcacaaaaa acgctgacca tcgaagtcgg tatgcaaaac tcgggcctgg ccgccgcgct
     1081 tgccgccgca cactttgccg ccgcgccggt cgttgccgtt ccgggcgcat tgttcagcgt
     1141 gtggcacaat atctccggct cgctgctggc aacttattgg gcggccaaag ccggtaaaca
     1201 taaaaaaccc taaacgcctt tcatcataat gccgtctgaa cggggcaaac aaaactggcg
     1261 gtttctgccc ggccccttca gacggccttt gtttgaaatc ggcggggacg attgcccggc
     1321 tgctccgatt cggctgcctg cgttcgcccc gcctttcaag ccgcctcact cccttacata
     1381 aaccacttcg acatcgtagt cgtcttcgtt ccaatcgtca tcgtcgtccg cgccgaattt
     1441 gtcctgccat tcctcttcat tgctcaaaga cgaatccaaa ccggcttcaa gacggaggac
     1501 agacaacaaa tgatatatgt cgtccggaat cggtgcttca aacgaaacgg tttcgccggt
     1561 tttcggatga gtgaaactta agcggtaggc gtgcaatgcc tgacgcgcac ccaaactttt
     1621 aacggcttct ttcaccgtgt cgccgcacgg atggcgcggg ttgccgtaaa ccgggtcggc
     1681 ggcaagcgga tggttggcct cacgcatatg gacgcggatt tggtgcgtcc tgcctgtttc
     1741 gagcgagcat tcgatatagc tgtgcgccag atagcgttcc aacactttga cgtgggtaac
     1801 ggctggtttg ccgccgaatt tgacgactgc cattttcagg cggttgtgcg gatcgcgtcc
     1861 gatttgggtt tcgattttac cgtcaaaggg aacgatgccg ttggcgacgg cgcggtaaat
     1921 gcgtttgacc gtgcgctctt gaagctgttg cacgagggaa ttttgcgccg gcagggtttt
     1981 ggcaaccacc atcagcccgc tggtttcttt gtccaaacgg tgtacgatgc ctgcacgagg
     2041 tacttggctc aattcgggac agtgcgccaa caggccgttg agcagcgtcc ccgtccagtt
     2101 gcccgccgcc ggatgcacca ccagtccggc cggtttgttg acgacgatga cggtatcgtc
     2161 ttcgtaaata atatccaaag ccataggctc tggaacaaac gccagatttt cctcactcgg
     2221 acggacggtt acacaaattt gctcgccgcc tatcattttg tctttgggtt gcgaaggttt
     2281 atcgtttaca ataaccgcgc cttctttaat ccatgatgtc aggcggctgc gcgagtagtc
     2341 gggcagaagt ttcgccaaca ccgcatccaa ccgcccgccg gcaagctcga gcggaacggt
     2401 cagaccgata cggttttcag tttcggaagc tgacgcaaag tctgaatcgt cgctataatc
     2461 ggattcatta tcaaaggaag tattctgcat gaaaaaaatt cttttaacgg tttcattagg
     2521 tttggcactg agtgcctgtg ccactcaagg tacggtcgat aaagatgctc agattaccca
     2581 agattggagt gtggagaagc tctatgccga agcccaggac gaattgaaca gcagcaatta
     2641 tacgcgggct gtcaagttat acgaaatctt ggaatcgcgc ttccccacca gccgccatgc
     2701 ccggcaatcc caactggata ccgcatacgc ctattataaa gacgatgaaa aagacaaggc
     2761 tctggcggca atcgaacgct tccgccgcct ccatccgcag catccgaata tggattacgc
     2821 gctgtatctg cgcggcttgg tgctgttcaa cgaagaccag tccttcttga acaaactggc
     2881 ctcgcaagac tggtccgacc gcgacccgaa agccaaccgc gaagcgtatc aggcctttgc
     2941 ggaactcgtc caacgcttcc cgaacagcaa atacgccgcc gatgcaaccg cacgcatggt
     3001 caaactggtc gatgcactgg gcggcaatga aatgtcggtg gcgcgttact acatgaaacg
     3061 cggcgcatat atcgccgccg ccaaccgcgc ccaaaaaatt atcggcagct atcaaaatac
     3121 acgctatgtc gaagaatcac tcgccatatt ggaacttgcc taccaaaaac tcggcaaacc
     3181 gcagcttgcc gccgatacgc gccgcgtgtt ggaaaccaac ttcccgaaaa gcccgttttt
     3241 gacgcacgcc tggcagcccg acgatatgcc ctggtggcgt tactggcatt aagcccatct
     3301 tcgtaatgcc gtctgaagcc gtttcagacg gcattttatg tttcaaacct cattcaaacg
     3361 gttttttctg ccaatagaat ttataccctc tcaaacgttg agccttcacg ccgccgcgtc
     3421 ccaagccgaa ttgcagtgcg cccgacaaat cggtacgcag cagtttgatg ccgtgtgcgc
     3481 ggacacggtt ctgcaccgct tcggtcggat gtttgtaggc gttggcataa ccgcttgaag
     3541 caacggcata ttcgggcgag acggcattga ggaacacgcc cgacgaggac gtattgctgc
     3601 cgtgatgtcc caaaaccaaa acctggctgt acaggttgcc tccatacttg ccgaccaggc
     3661 tttcctcgcc cttcgtatcc aaatcgcccg ttaccagcag tgccgcaccg cccgccacaa
     3721 cacgcaaaac acaacttttc ccattatcat cgatgttttt gcgttcagac ggcatcaaaa
     3781 actcaaaatc cacgccgtcc cattgccaac gctgttccgc acaatgccgc gccccctcat
     3841 aaaattccgg ctgcccggca taaatcccgc cgttcggtat cttgccgact gcctgaaaac
     3901 cgccgtcgtg gtcgctgtcg tgatgcgaca gaaccagctt gtccaaacgg cggacacccg
     3961 ccgcattcaa actcggcaca atccccatct gcgccgccgc tgccgttccg gtgtcaaaca
     4021 aaagatgatg atttgccgtc tgaaccgaca ccgacaaacc ctgccccgca tcccaaaccg
     4081 taaccgcaac ctcattttcc ggcacgctgg gtgaacggta aaacacaaac cctgccaaca
     4141 gcaacaccgc ccacggacgc aaacccaagc cgcgcggcag cagcaacagc aaagcggcac
     4201 acaccgccaa caccaacagc ggcaaaggtg cggaggcaac ggcaaactcg ggcgacacat
     4261 cggcaagcca caccaaaaac cgcaaagtat attccgccaa aaatgccccc aactgttgca
     4321 aaggcgcaaa cggcacgacc gaacccagca acgccagcgg cgtcaatatc caagaaaacc
     4381 acggaattgc caccgcattg accaaagggc tgattaaagg cagcgaagca aacagataac
     4441 cgagcaaaac cagcgacaac accgaagccg cccactgccc gcgcaaagca gtttgccgtt
     4501 ttccctcgtg caaacgcccc gagcacgccc atatcagggc cgccaccaaa ccgaaagaca
     4561 gccaagtccc cacacccaag accgccgacg ggtcgaacag cagcaccgcc gccaacgcct
     4621 gccaccacgt cgcccacgcc gacaatcttc ccctgcgcca agcccacgca aacgccgcca
     4681 acatcaaaac gctgcgctgc gtcggcacgg aaaaaccggc aagcagcgcg taaaacagcg
     4741 cgcctgcaca ccccgccgcc aaaacccacg cgcgcggccg ggcaggaagg cgcggggaac
     4801 aggcaagcag ccgcttcgcc agccacgcaa acattaccgc caccatcgta acgtgcaaac
     4861 ccgaaatact gaccaaatgc gtcagcccca acggccggaa cgcctgccac aattcggggc
     4921 gcaatgccga ctgttcgccc acgctcaacg cgcgcatcag cccgatgccg tctgaaagcc
     4981 cgccgtccgc atccgcctgc tgccaattac ggctgatgcg gctgcgccaa accgcaatcc
     5041 cccaaccgct tccgccatgc agcaaaaccc tgtccgcacc gaccgtcccc gcgccgccta
     5101 ttccattgga taatgcccac gcctcacggt tcaaaccgcg caggttcaac tccccgacga
     5161 cagggtgcac acgtgccgtt atccgccatc tgctcccgac cgcccattcg cgccgtttgt
     5221 agtccgacag cagcaaatca aacgtccgac caccgctgtc cacagccttt gccgcaaact
     5281 gcacacgccg cccgtccgac ctcggcatat ccgccacttc caccgtcaaa ggcacgcctg
     5341 aaacggcttc tgcccaccat tgggaagaca atgccgcctc cgtcctgaat acgccgtaag
     5401 ccgcgcccgc caacacgcac agcatcagac cggcaaacgc aaaacgcctt gcaaacacag
     5461 caaaaaccgc aaaagccgcc agccaaaaca gccaatgcgg cacgaccggc agcgcgaacg
     5521 aagccgccgc accgacaacc caacacggca aaaaccaacg acggaacacg tctccccacc
     5581 ccagatattt caaaaataaa cggataataa agcaaatccg aaaaaccgtc ataacaagat
     5641 ttgaaaatga tatagtggtt taaatttaaa tcaggacaag gcgacgaagc cgcagacagt
     5701 acaagcagta cgacaaggcg aggcaacgct gtactggttt ttgttaaacc actataagaa
     5761 tgcaaacgaa aagcctcagt attatccgag acaacggcaa actttccatc gtcattccca
     5821 cgaacccaca tcccgt
//
LOCUS       DAWFAN010000123         5807 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWFAN010000123 DAWFAN010000000
VERSION     DAWFAN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.123"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            131..565
                     /locus_tag="WGO65_001574"
     CDS             131..565
                     /locus_tag="WGO65_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ0327853.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(578..1441)
                     /locus_tag="WGO65_001575"
     CDS             complement(578..1441)
                     /locus_tag="WGO65_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0327854.1"
                     /translation="MELNEFFDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1449..2066)
                     /locus_tag="WGO65_001576"
     CDS             complement(1449..2066)
                     /locus_tag="WGO65_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ0327855.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2229..2843)
                     /locus_tag="WGO65_001577"
     CDS             complement(2229..2843)
                     /locus_tag="WGO65_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ0327856.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQIDLQEG"
     gene            3049..4821
                     /locus_tag="WGO65_001578"
     CDS             3049..4821
                     /locus_tag="WGO65_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ0327857.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAVENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            4818..5474
                     /locus_tag="WGO65_001579"
     CDS             4818..5474
                     /locus_tag="WGO65_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0327858.1"
                     /translation="MTIKIILIDDHTLFRSGIKSLLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1441 a         1634 c         1339 g         1393 t
ORIGIN      
        1 ttccgattcc gccgttatgt ctgcctgccg gacatacgta cgcattatta acaaaagtta
       61 accgcgataa taccatcttt cacacgtcaa tctagtatat ttcctaaaat ttccaacaag
      121 aggaaaagcc gtgccactgc ctgctccctg ccgttttgcc aaacctgccg cctctttttt
      181 aagtatggct ttgctttcct gccagctttc ccacgccgcc acggcttata tccccctgaa
      241 cgattttcaa ccgaactgcg acatacgccg gctcggactg acacagggtc agcacaatga
      301 actgcgtaaa atccgcgccg ccttcaaaat ggcgggcgac agggcgcgtt tgaaggttat
      361 gcattccgaa cacagccgcc gccggtctgt cgtcgaaatc atttcttcgg atgtttttaa
      421 tcggaacgag gcgcgcgatt atgtcgaaag ccgctatttg tccggtatgg attttgcggt
      481 ggacgaattg gaaatccaac accgcttctt ccatatcctc acaccgcaac agcagcaaat
      541 gtggctttct tcctgcctca aataatcccc gaaacgctca caacgcccgt tgtttcggca
      601 gcctgcccgc ccagtcgcag gcaaactgcc acgcggaacg tcccgaacgg ctgccccgcg
      661 tctgcgccca ctgcaatgcc gccatctgtg cggtttcatc atacggcacg ccgaaatctt
      721 ccagccaatt ttgcaccgcc gccagataat cgttttgatc gaacggataa aaactgagcc
      781 acaatccgaa tcggtcggac agggatattt tttcttccac cgcttctttc tgatggattt
      841 ccccccgcat ccccgtcgta ccggcattct cgtcaaaata ttcgggcatc aggtgccgtc
      901 tgttggaagt cgcgtaaacc aaaacgttgg cgcaacgttg agacagaccg ccgtctaacg
      961 cggttttcaa tgccttatag gtttcatcgc cgctttcaaa cgacaaatcg tcgcaaaata
     1021 cgataaattt ttccggacgt tccttcaaaa gcgtcaacag gtaaggcagg ccgattaaat
     1081 cgcttttatc gacttcgatc aggcgcaatc ccttatccgc atattcgtgt agcagggctt
     1141 tgaccagcga ggatttgcct gttccgcgcg cgccgctcat caatacattg ttcgcgggtc
     1201 tgccgacaat gaactgttcg gtattacgca ccagcaattc ggtttgcctg ccgactcccg
     1261 ccagccttac caagggaaag gtgtgcggat cgggcaagtg ttccaaaaaa ccttttttgc
     1321 ccgcactctg ccagcggaag gcaagcgcgt tccaatccgt atgcccgggt tcgggcggaa
     1381 gcacggcatc caaacgccgc aaaacggcat aggctttatc gaaaaattcg ttcaattcca
     1441 tctctacctc actttgcata tctttgcgcc atcagccgtt cgacggtgtc gacgattgcc
     1501 tgcgtattcg gatcgatttc gatgttgatc ctgtcgccga cctttctgct gccgaacagc
     1561 gtccgttcca aagtttcggg aatcagatgg acattgaaac ggccgtcttc gactttgcct
     1621 atggtcaggc tgcaaccgtc caagccgacg aaccctttgg tcaggatata gggtttgagt
     1681 tcatgcggga gcgaaaacca aaccgtgcgg ttgaacccgt cccgttcgat ttcgacaata
     1741 ggcacggttg ccataatgtg tccgctcatg acgtgtccgc cgatttcgtc tccgaaacgc
     1801 gccgcccgtt cgatgttgac gcaatcgcct tccttcagca gccccaaatt ggtttttgcc
     1861 aaagtttccg ccattaaatc gaaactgacg cggtttcctt cgatttcggt aatcgtcagg
     1921 cagcagccgt tattggcgac cgatgcgccg cgttgcagat tgtccgccgc ctcttgcgga
     1981 agctcgacga cataagtgtg aaatgcctcc gacgggcggt ggattgccgt cagttttccc
     2041 aatccttgaa caatgcctgt aaacataatc ctgtttccct gtgtcggtaa aaatggtgca
     2101 aattgtagca tctccccgcg aaaaatgccg tctgaaatgc cttcagacag cattatgcct
     2161 ccgattcggg caaaaaccgc ccggtatggc ttgacctttc ctttccacgc cggtcggcgg
     2221 tcttgccctt atccctcctg caaatcgatt tgcgtgttca agtcggcaaa atgcccgtca
     2281 aactcgaatc tgaccgaccg cgccctttgc tgctgcaacc agcttcttaa tgttttcata
     2341 cccgaaaaca gatagggaat cgcgctttgc agaatctgcg ggcggatata cataatgttg
     2401 tagtgcatcg ttatcggcgt ttccacataa tacgcattgc acaacggtgt gcgtttcgac
     2461 acggtttcaa acctcgccac caaatcgtcc ggcagatacg gcatatcgca cggcacaacc
     2521 aaaagccagt cagcagtcgc caactgcaaa tcgttggctg cggtacacaa tgccgaaagc
     2581 gggccgaaat gctgccactg ccgcgcatcg ggaaaaatat gcggacttct tcgagcatat
     2641 tcttccaaat tccggttggt gctgatggcg atatggctga cctgcggcct gaccctgtcg
     2701 atgacatggt ctatcagtgc cttacccccc aaaagagcaa gccccttgtc ctcgcctccc
     2761 atacggctcg cctgaccgtc ggccagtatc agggcaaaag ttttcattgc ggatgttctc
     2821 ttggaaaagt tcgaggtttt catgattgca gtctgccgtt cccaatgact caaaatgccg
     2881 tctgaagcag atggcaaata aattcatatt atctgaattt tatcataaca tgatttaaca
     2941 ctgaagcggt gcggattaag ttttccttaa ccaattcttt ctgagcagtt gtatctaatc
     3001 ccaaagaatg atattgtttg cattatttag aacaattttt cgccgagcat gatactgcca
     3061 gcccgttttt cagacggcat cagcctttcc ctacgcctga aactcctgac cggagtgtgg
     3121 gtcgggttgg cggcattgtc tgtcgttttg acactgctgc tctctttgcg tctggaaaac
     3181 gcggcctccg tcatcgaaga ggcgggcaac ttgagaatgc aggcataccg tctggcatac
     3241 atggcgggtg aaggatctcc ccgctcccaa atagacaatc aggttgacga atttgaaaaa
     3301 agcctgaatc gtatttcaca aagtgatgcc atccatccgc tgattccttc ggacacccct
     3361 cttgcttatg atttgataca atccatgctg attatagatt ggcaggcaca catcctcccc
     3421 ccgctccagt cctaccggcg accgactcag gtcgatctct accgctttgc cggaaacatc
     3481 gaactgtttt tgcaggcatt ggaaaatgcc aacgaaaaaa acacatggtg gctcaggcgt
     3541 tttcaatggg caattatgtt gatgacgctg gtgtcgtctg tactgatgct gttttggcac
     3601 cagatttggg ttatccggcc gctgcaggcg ttaagggaag gtgcggaacg catcggacgg
     3661 aggtgtttcg atattccggt tcccgaaggc ggtacgccgg aattcaaaca ggtcgggcgt
     3721 tgtttcaatc aaatgggcgg caggttgaaa attttatatg atgatttgga aggacaagtc
     3781 gccgagcaga cacgcagtct cgaaaaacaa aatcaaaacc tgaccctgct gtaccaaact
     3841 acacgggacc tgcaccaatc ctacataccg caacaggctg cagaacattt tctaaaccgt
     3901 atcctgcccg ccgtaggagc agattccggc agagtttgtt tggacggcgg atccgatgtt
     3961 tatgtttcca ttcatcatgc ggattgcggc acagcagctt cggatttggg gaagtaccat
     4021 gaggaaatct tccccattga gtaccagaac gaaacattgg gcaggctgtt gctcagcttt
     4081 ccaaacggca tttctcttga tgaagacgac cgcatcctgc ttcaaacact aggcaggcaa
     4141 ttgggcgtat cgcttgccgg cgcaaaacag gaggaagaaa aacgcctgct tgcagtattg
     4201 caggaacgca acctgattgc gcaaggatta catgacagca tcgcacaagc attaacgttc
     4261 ctaaacctac aggtacagat gctggaaacc gcctttgccg aaaacaaacg ggaggaagcc
     4321 gtagaaaaca tcggcttcat caaaacaggc gtgcaggaat gttatgaaga tgtccgcgaa
     4381 ctgctgctca acttccgtac caaaatcagc aataaagaat ttcccgaagc cgttgccgac
     4441 ctattcgccc gctttacgca acaaaccggg ataacggtcg aaaccgcctg ggaaaacggt
     4501 tcgttcctgc cgcctcagga agcgcagctc caaatgattt ttatcctgca ggaaagcctg
     4561 tccaacatcc gcaaacacgc ccgcgccacc catgtaaaat tcaccctttc cgaacacggc
     4621 ggacgcttta ccatgaccat ccaagacaac ggacaaggtt tcgacacgga gaaaatagga
     4681 gaacccacgg gcagccatgt cggactgcac atcatgcagg agcgtgccaa acgcatccat
     4741 gccgttttag aaatccgttc ccaagctcaa cagggaacca ccgtctcatt gacggttgca
     4801 tctgaagaaa gcttgaaatg accattaaaa ttatcctgat agacgaccat accctcttcc
     4861 gcagcggcat taaatccctt ttgtcgcgcc aacatgattt tgaggtcatc ggcgaagccg
     4921 cagacggcct ctcgggcatt aaaatgatca accggctcca acccgatgtc gtcctgcttg
     4981 accttgatat gcccggtatg aacggacgcg aagcactctc ccaaatcatc agcatcaatc
     5041 cgcagcaggc agtgattatg ctgaccgttt ccgaagacaa cgacgacttg accgaatgta
     5101 tgcgcatcgg cgcgcgcggc tacctgctga aaaacatcaa cgccgacttt ctgctcgaaa
     5161 gcatacgcaa agccgccgaa ggcgataatg tattctcgcc cgagatgacc gccaaactcg
     5221 tcaaaagcct gatttccccc caacctgccc aagggacgca ggcactctcc tcacttaccc
     5281 ctcgtgaact ggaaatcttg ggctatctcg ccgcaggaca cagcaacaaa atcatcgccc
     5341 gccacctcga tcttgccgaa tccaccgtca aagtccacgt tcaaaacctg ctccgcaaac
     5401 tcaacctcag cagccgggtg caggccgctg tttacgccat ccggcacaac gtcccccaac
     5461 ccgtgctgga ataaggcggg ttcagacggc atattagggg ttttaatccc cgtacggtca
     5521 ttcggataac agaccaagca tgtaagttta tgcccccata agtacgcttg gcatagcagt
     5581 aatattgttc ggttcagtgt tttccgtttg cccctatctg atactgcaat atcagctatg
     5641 ccgtctgaaa acgcatcatc atgatatttt cagacggcat aataaaaagc ggaaatacta
     5701 atgcagggta aaatgttcca ttccgaatcc cataaatata caatggctta tatcgtttaa
     5761 gcatgtgtaa cccaccctca tatcaatcaa tatatagtgg attaaca
//
LOCUS       DAWFAN010000124         5737 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWFAN010000124 DAWFAN010000000
VERSION     DAWFAN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.124"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(85..1131)
                     /gene="recA"
                     /locus_tag="WGO65_001580"
     CDS             complement(85..1131)
                     /gene="recA"
                     /locus_tag="WGO65_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ0327859.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGIEMHIT
                     EGTQDETDGERPEE"
     gene            1385..2149
                     /locus_tag="WGO65_001581"
     CDS             1385..2149
                     /locus_tag="WGO65_001581"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ0327860.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2169..4184
                     /gene="rep"
                     /locus_tag="WGO65_001582"
     CDS             2169..4184
                     /gene="rep"
                     /locus_tag="WGO65_001582"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ0327861.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVSDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(4252..5310)
                     /gene="dinB"
                     /locus_tag="WGO65_001583"
     CDS             complement(4252..5310)
                     /gene="dinB"
                     /locus_tag="WGO65_001583"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ0327862.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLIARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT         1412 a         1508 c         1536 g         1281 t
ORIGIN      
        1 caaaactcaa atcaagccgt tcggaggctg ctcaaaaaaa acggtacttc gcaacagaag
       61 taccgtttat cgggatttca ggttttattc ttcggggcgt tcgccgtcgg tttcgtcctg
      121 cgtcccttcg gtgatgtgca tttctatgcc gttgagggcg cggatttttg cgtcgatttc
      181 gtcggcaatt tcgggatttt ccttcagcca gacgcggacg ttgtctttgc cctgaccgat
      241 tttcgcgccg ttgtagctgt accacgcgcc ggatttgttg atgatgtcgt ttttcacgcc
      301 gatgtcgatc aattcgcctt cccaactgat gccttctccg tagaggatgt caaactctgc
      361 ctgacggaac ggcggcgcga ctttgttttt gatgactttg acgcgggttt cgttgcccaa
      421 tacttcttca ccttttttga tggatccggt gcggcggatg tcgaggcgga cggaagaata
      481 gaatttcagc gcgttgccgc cggtcgtggt ctcggggctg ccgaacatta cgccgatctt
      541 catccggatt tggttgatga acacaaccag cgtgttggtt tttttgatgt gtccggtcag
      601 tttgcgcaaa gcctggctca tcaggcgcgc ctgcagtccg acatggctgt cccccatatc
      661 gccttcgatt tcggctttgg ggacgagtgc ggctacggaa tcgacgacta ccatatctat
      721 gccgcccgaa cggacgagtg tgtcgcagat ttccaaagcc tgttcgccgg tatcgggctg
      781 ggacaggtaa agctcttcga cttttacgcc gagtttgcgg gcgtaaacgg gatcgaaggc
      841 gtgttcggca tcgacaaagg cgcacacgcc gccgtttttc tggcattggg cgacggcttc
      901 gaggcagagg gtggttttgc cggaggattc tgggccgaag atttcgacga tgcgcccgcg
      961 cggcagaccg ccgactccga gggcgaggtc taatccgagc gatccggtgg aaatgacttc
     1021 gaggttttct tcctgctggc tgccgtccat tttcatgatg gcgcctttgc cgaaactttt
     1081 ttcgatttgc gccagtgcgg cggcaagtgc tttgcttttg tcgtctgaca ttggggttac
     1141 tccggaacaa atgcggtatg tgggatgcgg cgcaacacgg gctgcggcgc gggatgtgta
     1201 tcgttttccc gatgtgcggg ctatcggtaa tgctgcttca cgaggttgcc attatcgcat
     1261 atttccttgc ttgccgatat gcgccaggac gcggcggctt gtgccggaat ggaatctgga
     1321 tgccgtctaa aaggcggccg gctttgttat aatggcggct gttttttctg tgtgtgcctg
     1381 ttttatgtgt tcctgccttg ttgtcaaaaa taccgttatc ggaagcggac gcaccaaaat
     1441 cgccgtgccg cttgtcgccc gcgatgccgc cgaactttcc gccgtacttg agcaaatcaa
     1501 aaatatgccc ttcgatattg cggagttccg cgccgacttt ttggaatgcg cgggcagtat
     1561 cggcgaaata ttgcaccaca cgcagaccgt ccgcgacgcg ctgcccgaca agccgctgct
     1621 gtttacgttc agacggcatg gcgaaggcgg ctcgttcccg tgttcggacg attattattt
     1681 taaactgctc gacgcgctga tcgaaagtcg cctgcccgac attatcgata tcgagctgtt
     1741 ttccggcgaa accgccgtcc ggcgcgctgt ggcgaatgct caaaaaaacg gcatcgccgc
     1801 cctgctctgc aatcatgagt ttcaccgcac gccgccgcaa gaagaaatcg tatgccgtct
     1861 gaaacagatg gaggactgcg gcgcggacat ctgcaaaatt gcggtgatgc cgcaaagcgc
     1921 ggaagatgtg ctgactttgc tttccgccac gctcaaagcg aaagagcttg ccgccaaacc
     1981 gattgttacg atgtcgatgg ggcagacggg ggcggtcagc cggcttgccg gacaggtgtt
     2041 cggctcaagc atcacgttcg gttcgggaac gcaaaactcc gcgccggggc aaatcggcgt
     2101 atccgccctc cgtgcgacac tcgactgcct cgaaaacggc gcagactgat ttcagacagc
     2161 atcaaaacat gatgaaactc aatccccaac agctcgaagc cgtccgctac ctcggcggcc
     2221 cactgctcgt ccttgccggt gcaggcagcg gcaaaaccgg cgtgattact caaaaaatca
     2281 agtatttgat tgtcaatgtc ggctatctgc cgcataccgt tgccgcgatt acctttacca
     2341 ataaagccgc cgcggaaatg caggagcgcg tcgccaaaat gctgcacaaa tcgcaaacgc
     2401 gcgggctgac gatttgcacg ttccactctt tgggcatgaa gattctgcgc gaagaggcga
     2461 accatatcgg ttacaaaaaa aacttctcca ttctcgattc taccgacagc gcgaaaatca
     2521 tcggcgaact cttaggcggt acgggcaaag aagccgtatt caaggcgcag caccagattt
     2581 ccttgtggaa aaacgattta aaaacgcctg aagatgtcgt tcagacggca tcgaacattt
     2641 gggaacaaca aaccgcacgc gtgtatgcga gctatcagga aaccttacaa agctatcagg
     2701 cagtggactt cgacgacttg atccgcctgc ctgccgtgct gttgcagcaa aacagcgaag
     2761 tgcgcaacaa atggcagcgg cggctgcgtt atctgctggt tgacgaatgc caagatacga
     2821 atacctgcca atttacgttg atgaaacttc tgaccggcgc ggaaggtatg tttaccgccg
     2881 tcggcgacga cgaccagtcc atctacgcat ggcgcggcgc gaacatggaa aacctgcgca
     2941 aaatgcagga aaactatccg cagatgaagg tcatcaaact ggagcaaaac taccgctcca
     3001 ccgcgcggat tctcaaaatc gccaacaaag tcatcgagaa caaccccaag ctgttcacca
     3061 aaaaactttg gtcgaaattg ggcgaaggcg agcccgtcaa agtcgttgcc tgccaaaacg
     3121 agcagcacga agccgactgg gtcgtcagcc aaatcgtcaa acaaaaactc atcggcggcg
     3181 acaaaaccca atatgccgat ttcgccgtgt tgtaccgcgg aaagcatcag gcgaggattt
     3241 tcgaggaagc attgcgcggc gcgcgcatcc cctaccgtct ctccggcgga caaagctttt
     3301 tcgacaaagc cgaaatcaaa gacgtgttgt cttatgtgcg gctgcttgcc aaccccaatg
     3361 acgatcccgc ctttctgcgt gccgttacca cgcccaaacg cggcatcggc gatgtcacgc
     3421 tgggcaagct caacacttac gcgcacgaac acgaatgcag cctgtatgaa gccgcacaaa
     3481 acgaagaagc ccttgccacg ctgaacaata ccaaccgcca acacctgcaa gcctttatgg
     3541 atatgttcgg cagctaccgc gccaaagcgg aaaccggcga agcaggcgag ttcatcaaca
     3601 gcctgctcga agaaatcgac tatgaaaacc atttgatgca aaacgaagaa ggcaaggccg
     3661 gcgaaatcaa atggcgcaat gtcagcgatt tggtatcatg gtttgcgcga aaaggcgggg
     3721 aagacggcaa aaacatcatc gaactcgccc aaaccgtcgc cttgatgacg cttttggaag
     3781 gaaaagacga agaagaaacc gatgccgtct cgctatccac gctacacgcc gccaaaggtt
     3841 tggagtatcc gtatgttttc cttgtcggtt gcgaagaagg cgttttgccg cacaacgaca
     3901 gtatcgaaga gggcaacgtc gaagaagaac gccgcctgat gtacgtcggc atcacccgcg
     3961 ccaaacgcca actcacgctg acccactgcg tcaaacgcaa aaaacaaggc atatggcagt
     4021 tccccgaacc cagccgattc atagacgaaa tgccgcagga agatttgaaa atcctggggc
     4081 gcaaaggcgg cgaaccgatt gtcagcaagg aagaaggcag gcgcaacctt gccgatataa
     4141 tcggaaggct cgacaaccta aaaaaaagcg gcgcggcgga ttaaaccgga gccgcaatgc
     4201 cgtctgaagg cttcagacgg catatttttt ggacggcgcg cgtaaagcgg tttacgccca
     4261 caaatcctgc tgctggtttt tcggcacaag atgccccacg ccgataccga taaggcggaa
     4321 cgcgtcttcc gtctgcggcg ggacgcgcgc tatcaacatt tgcgcagcct gcagcagagc
     4381 tgcgcagtcg ggcaatacgg aggaataagt cagtgtgcgc gtgatgatgc ggaaatcgta
     4441 ggtcttcagc ttgagcgtta cgctttgggc ttcgacgttt ttgcgcgtga tttgccgcca
     4501 caggtcttca gcaagatggg gcaggtgtcc ggcagcctgc tcgagcggca ggtcttcagg
     4561 cagggtgatt tctgtggaga tttggaggcg ttcgcgttcg gctttgacgg ggcgttcatc
     4621 cgtaccgcgc accaaatcat agaggcggta tccgtagcgt ccgaaatggt ttaagagctc
     4681 gccgcgctcg aaacggcgca agtcgcccgc cgtccgcata cccagcgact gcattttttt
     4741 cagcgtaacc ttgcccacgc cggggatttt gcccaaaggc agggtttcca aaaatgccat
     4801 gactttgtgc ggcggcaaca caaactgccc gttcggcttg cgccagtccg acgcgatttt
     4861 cgccagaaat ttgttcggcg cgatgcctgc ggatgcagtc aaacctgttt ccgcaaaaat
     4921 ggcggcacgg atttctttgg caacttcact ggcgtaaggg atgtttttga aattacgggt
     4981 aacgtcaaga taggcttcgt ccagcgacaa gggttcgatt aaatcggtat aacgcctgaa
     5041 tacggcgtga atctgcgcgg aaacctgacg gtacaaatcg aaatgcggcg gcacatacac
     5101 cgcttgcgga cacagccttt tcgccgttgc caccgacatc gcggaatgca gcccgaactg
     5161 ccgtgcctca tacgatgcgg cgcaaatcac cgaacgcgcg ccctcccacg cgacgaccac
     5221 cggccgccct ttcaaatgcg gctgttcgcg cagctctacc gatgcgtaga acgcgtccat
     5281 gtcgatgtgg atgattttgc gtgaagacat cggctctgct gaggataaaa gggatattct
     5341 actgccggca tcgggcaaat tccaaatata ctaacaggtt aaatacaaaa aagttggctg
     5401 gattaatttt ggaagcttat aaaaataatg gtttttatca agcagaaaga aacagcaatg
     5461 gaaattatga tatcgtaaga aaaaaattat ctgaaaaaag attaccaaaa tacaagcaat
     5521 atattaattt actcagataa taatagtgac tgatttaaaa ttcagcagcg tagaaaacaa
     5581 atcagagcac aaatttcagc cagacaatgg agaagataaa agatgaataa aaatatactt
     5641 tatatatttt ctttattaat cgctattgtt atttctttta tatttgaaaa gaatgtaata
     5701 agcaaaataa gttttaatta taataaaaaa gaattct
//
LOCUS       DAWFAN010000125         5695 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWFAN010000125 DAWFAN010000000
VERSION     DAWFAN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.125"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(198..410)
                     /locus_tag="WGO65_001584"
     CDS             complement(198..410)
                     /locus_tag="WGO65_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0327863.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(422..703)
                     /locus_tag="WGO65_001585"
     CDS             complement(422..703)
                     /locus_tag="WGO65_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327864.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(741..944)
                     /locus_tag="WGO65_001586"
     CDS             complement(741..944)
                     /locus_tag="WGO65_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327865.1"
                     /translation="MNIIEQEMKHSSLRKVDVEIAKIIADAHKINAESVKIAQESRWY
                     PMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1024..1596)
                     /locus_tag="WGO65_001587"
     CDS             complement(1024..1596)
                     /locus_tag="WGO65_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEZ0327866.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1575..1886
                     /locus_tag="WGO65_001588"
     CDS             1575..1886
                     /locus_tag="WGO65_001588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327867.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSVLSSVLPASLRRLRVGLMLTASSLRYSVARLMPNSCACCLRYAERAPRPIAS
                     A"
     gene            complement(1908..2783)
                     /locus_tag="WGO65_001589"
     CDS             complement(1908..2783)
                     /locus_tag="WGO65_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ0327868.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2828..3118)
                     /locus_tag="WGO65_001590"
     CDS             complement(2828..3118)
                     /locus_tag="WGO65_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0327869.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3130..3309)
                     /locus_tag="WGO65_001591"
     CDS             complement(3130..3309)
                     /locus_tag="WGO65_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0327870.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3423..3800
                     /locus_tag="WGO65_001592"
     CDS             3423..3800
                     /locus_tag="WGO65_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0327871.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            3829..4074
                     /locus_tag="WGO65_001593"
     CDS             3829..4074
                     /locus_tag="WGO65_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327872.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4308..5276
                     /locus_tag="WGO65_001594"
     CDS             4308..5276
                     /locus_tag="WGO65_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327873.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
BASE COUNT         1342 a         1290 c         1427 g         1636 t
ORIGIN      
        1 ggcgaaaaca gctgaaaacc tgtgtttggg tttcggctgt cgggagggaa aggaattttg
       61 caaaggtctc agagtgagtt tattttgggg cggcggcagg tcgggggcaa gcggcgtggg
      121 gcttggttgt ggtttttagg tttgggggtg ggtaaaaaat gccgtacccg gcgggcaggc
      181 ttcgcactct gaaggtttca gacggcgttt gttttttctg tccaatcgag gaactgccgc
      241 catttttcca gcggcatatc ggcccgccgc gtttcggtat cgggttcggc ttcccagcgg
      301 cctacctgta tgtagtgctt caccccgacg atttgcgcca actcggcctg tgtcagtttg
      361 cagcggttgc gcagggtgcg aaggttgtaa ggcgtgtagc cgagttccat gtcgttgcgg
      421 tttattttgt ttcgcatatt tttttgactg cccggcggca ggtttcggta aggacggcgg
      481 caaatcgggc ttcgtctgcc ggttttccgt cgaaaaaaat aaaatcgtaa agggtgatgc
      541 cgttttcgga atggcggtaa tgcccgcccg ggcattcgcc gtctgtctgc ctgtggattt
      601 tggcaatcag gcggggatag cggcaatggg taatgaaacg gcttgcgccg tcttgcccga
      661 taatccattc ggggtagcgg ttgaatagtg cggctctgtt cattttgttc gggggataaa
      721 gcccctcgcg gggcttgtgg tcaggcaaat ttgaatatca gtgccaacac ggcggcgatg
      781 gcggtaacca gcccggtggc cgccatcatg ggataccatc gggattcttg ggctattttt
      841 acggattcgg cgttgatttt gtgcgcgtct gcaatgattt tggcgatttc gacatctact
      901 tttctcagac tggagtgttt catttcttgt tcgattatgt tcatcgtact tcctttcgtt
      961 tttggcggtt gccgccgctt gtcggatggc aggatgtctg ccatgtgtat atattgatac
     1021 cttttaggtt ttattgcaag tgttttgggc ggcggcttcg tatgcttggc ggtggcggcg
     1081 gctgtaccat tcggcgagtt cgcgccgctc ttggaggcgg tagctgtcgg cggtcaggta
     1141 gtggtgcagg cttgagaagc cggcgcgttg gagggcggcg cggctgatgt ggccgagggc
     1201 gaggtctgtt gtgagggtgt cttttccggc ggtcaggctg atgttggtga agaggtggcg
     1261 gaatccgtgc attgtgtgtt tggatttgcc gggggtgctg ccgtcatagc ccagtcgtcg
     1321 gatggcgttg tgggcgaatt tgatgctgat gtggtcggga tggggggcgg gtttgcgccg
     1381 tgggcggatg ccggggaaaa ggtggatgtt gtcgccggtc tgtgtgtgca gctctcggag
     1441 tatttctacc gcccagtccg acagtgggac ggtaaagggg tgtttggtct tcatgtcggc
     1501 ggcggggatg tgccataacc gggcggtgag gtcgatgtct tgccagcggg cggaaagcag
     1561 tgcggacgga cgggatgact tccccggttt ccccatcaat ccgcgccgcc tcgatttttc
     1621 cgttggcgcg cagcatttcc accgcctgcc gcaccgacat caaccgcttg ccgttgcgcc
     1681 tcatcgcctc caccaaaacc gccgaaatca atttggcttc ttccgtttta agctccgtct
     1741 tgcccgcatc gctgcgccgt ttgcgcgtcg gcttgatgct gaccgcctcc agcttgcggt
     1801 atagcgtggc aaggctgatg cccaattcct gcgcctgctg cttaagatat gcagagcgtg
     1861 cgccgcgtcc cattgcttcc gcctgattct cgactgcctt aagacgctca atcattgccg
     1921 gattcatcgc cttctcccgt ttcaccgccc aaccattccg gcacattgtc tgtcggtgct
     1981 tcagtcggca gggcatagct ttcgcgcagt tgctcgcagt ccaaaataat ttgattgagc
     2041 gtgccgacca tctttgcctg atggctgatc tcgtgtgcct cactgtgcgc attaagttgg
     2101 tcgaacaaat ctttcaaacg gctcacttga ctgcggatac cgacctcaag gcttgttaac
     2161 tgcatcgcca actcactgcc cacgtcttcc gccttcggct ctctgacaac ggtttgcttc
     2221 ttagccagct tctcggtcag ctcatcaatt ttggctgttt tggttttcat cacttcgtct
     2281 ttggcggcga ggttttcacg gctttcgcgc agggcgacgc gcagctcgcg caccgtcatt
     2341 cggtccacat cgtcaaaggt catgccgttg acttcttctc cttcggcaag tcccaccaac
     2401 gtaacgtctt cttcgaccag cagctcaagc agcttcgact tgcccaaatc catcagcttc
     2461 ggcgcggctt tctgcatttg cggggtcgca aagcggcgag tggctgacat cagacgtgat
     2521 gtttctgcga tacccagccc aaattggctt ttcacaattt ccataaaccg tccgtgttcc
     2581 gtatgctctt ttaaaataat cagcgcacgt cccagttcga acatgccttc catcgtctgc
     2641 cgtactgctt gacgaccgcg ttcaacccaa cgctcttcgc tataagtctc gccgttaccc
     2701 cactgctcca tcaccagcac actgcgcatc gctgcctgat tgcttacttt gtcggttgcg
     2761 ataatttcaa tttctgtatt cattttttat ctccaaagtt tccgacgtcg gaaactttca
     2821 aaatccgtta atccacatcg acccgcttgc cgatttcggc aatcttgctt tgcagccgtt
     2881 catgctgctg cctgaaccgc tcggcgattt gcagggtttt gatgccgtag gcgtagttgc
     2941 cgttttcaag tttgatgacc aatcccgagg caaccaaatc atcaatatcc ctgctgactt
     3001 gcgatggcgt cagccccagt ccgaccgata aatccttatt gctcagaccg ataatcggat
     3061 gctcgtcaag cgcgataaag accctcaata gccgttgtac ccttttactt tctgccatcc
     3121 gcatcctcct tatttcagtc ccagcttctt ggcaatttca tgccccttgc cgtaattgcc
     3181 tttgcgctgt ccgccgacca ccagatacac atcgcgcggc ttaaagccgt tctctcttgc
     3241 ccactgcgcc agcgtttggc cgttttgggc aaaattttct tttagttttt caacagtata
     3301 gccatagata gccctttccc attgtgataa aatagtgaac tatttatgca ctagaaatta
     3361 attagtgcat taccaatgcg tgaattatga gattttaaaa tctcttttac aaggattatt
     3421 ttgtgatttc taaatctctt tttggtaatc gtttgaaaga aaaaagaaag tttttagggc
     3481 ttacccaagc acaagctgct gaaaaagcag gtattgagcg cgaaacatgg ggcaaatacg
     3541 aacgtggcgt atttatgcct agtggagatg tattgctctc ttttttgaat atgggaatag
     3601 atgtgagcag cttatttgct gctgaacagg gaattaggcc gtctgaaaat tctgaaatta
     3661 gtaaggaaga gtcagagtta ttagggtatt accgccaagc cagcgataac ggcaaatttg
     3721 tcattctcag tgtggcaaaa ggcgcagaga aaaaagcagc cgaaaatact caagtttcag
     3781 ccacgcataa aacagcgtga tttttaaatc caaaatggag acagtcttat gtttgacttt
     3841 gaacaacaaa tcaaatgggg tgagcgagcg gaagaaatcg ttaaagaggc agccacccag
     3901 aacaatatcg aaattcccga acctttggct tcggcattag ctaaggcggt aaaagtgcat
     3961 tatctaagcc aagctggtgt gttttctttg gtagaagcct atgcagacac ggtaaatcca
     4021 actgaaaaag aagtagatta ccaagcaatc ggcaaagagc tatttgaaaa ataaaaaatg
     4081 ccgtctgaaa ttttcagacg gcatttagga cactccgaca gtcgggctgc tccggtgttc
     4141 tgaaacggat attaagcaat tgaagcggcc gtgcatttta aaacgcatta aaaactaccc
     4201 cgacatacag gtcatcgggc aggtggttca ggtgttgaaa gatttgaatt gatgccgtct
     4261 gaatttattt tttcgacaca gatattaaaa gagaaaggac tgtacaaatg gcaaaaaaaa
     4321 ctactaaaac tattcattat ttacgtgcgc agcaaaacca tagcttgttc gatttagagg
     4381 tagcattaag aagggttttg tcagctgctc ctactgttca agatacacaa attgaacaat
     4441 acaatcagct tacccaaatc atgcatagaa ctttggatcc taaagatgta aaaggcaatt
     4501 caattggtgg attactgata cacattggca gcggtacaaa agatgaacat atccgcacta
     4561 tgagcaatag acctgtacag caagatgatc atggtggaac acaagctcct ccaagtggat
     4621 attccttttt gcgtaaagag gcttttttgt atattgttgg acaccatgta attttttgcg
     4681 gacatggttt tttgtcagca tcaacggttg cttcttattt aagtttactt agcaataaat
     4741 tgagagagaa caatccggat attgttcaat ttaatattga attcaaagca gtaggcaatt
     4801 gcgacaagtt gtcattgata caacaacatg gggtaaaaag tattctttta gatgcttcgg
     4861 catatcaatt atccagaaac agactgtatc aagatagccg ttcaaccatt gctaaagcat
     4921 tagggaaagt tggtagtgta tttacaagcg aattgagtga tgaggaattg gaagcacaat
     4981 ccgaaattca tatcaattta gaagtcttgc taaacggtaa ttcgagagcc agtattgaag
     5041 cgcaatcgct gatgcaggag caggcagagg aaattattga tgatgaaacg gttaatcaag
     5101 gattttccat taccacgcaa caaggtgaag tgattcagcc ctctgatgta aaactgtcta
     5161 aatctgttag aataggacga tacgatgaag ccaactcgtt actccctgac tcggcattta
     5221 cagcaatcag tgaatatttc ctagaactcc aaagcagaaa cttaactgaa caatgaaaaa
     5281 atatctgccc ttactacgtt ttttgttgat tgcttgtata tcagcatttt tagcatggaa
     5341 agggcaacct tttgttcatg ggaactagaa ggccgttgat ttaattatta acgtatttgc
     5401 aatcttagcc ggttttttaa ttgccattat gacattgttt agtgatatga ggtttgatga
     5461 agatgcaaat tggcgacaaa ttcaaattcg tgaaggcgta caagaacaac ggtatataaa
     5521 acattcctta cttttttaca catatcttgc cgtattagtt tgtgttttta ttgtcatctt
     5581 gttggctcat aaagaagaat acaaaaatgg accagccatt ttttggcttg aacggagcta
     5641 tttattttta gcctgtattt ccatttttta ttctgtattc ttacctggga attta
//
LOCUS       DAWFAN010000126         5692 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWFAN010000126 DAWFAN010000000
VERSION     DAWFAN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.126"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            97..813
                     /locus_tag="WGO65_001595"
     CDS             97..813
                     /locus_tag="WGO65_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0327874.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="WGO65_001596"
     CDS             962..1864
                     /locus_tag="WGO65_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ0327875.1"
                     /translation="MDSLMTLLSVLVPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1907..2680
                     /locus_tag="WGO65_001597"
     CDS             1907..2680
                     /locus_tag="WGO65_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ0327876.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEAGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="WGO65_001598"
     CDS             2792..4480
                     /locus_tag="WGO65_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ0327877.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="WGO65_001599"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="WGO65_001599"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ0327878.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWQLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELGGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1323 a         1541 c         1578 g         1250 t
ORIGIN      
        1 atttgtgcaa tgctttaatt cctctgacac gatttgaaac acagccgcct taaaacatct
       61 taaaaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccctgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc cggcaaaaag
      241 ctgaccgaat ccgaactttg ccgtcagatg gtgtgttcgc gcaacaccgt ccgcggcgcg
      301 ctgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc agatttgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgataa ggcgcgaaga agaggcttcc ggcaggggcg accgcgtcgg ctggaaccgc
      541 ctgtccaatg ccttccacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggaacgct tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca tatatcatgc ggcaataaag gaggtcagga
      961 gatggacagc ctgatgacat tgctttcggt attggtaccg atgttcgccg gattttttat
     1021 ccgtgtgccc aagccttacc tgcccgcttt ggacaaggtg ctgtcggttt tggtgtatgc
     1081 cgtgctgctg ctgatcggcg tatcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcggggcga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggcgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcattcgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaagcgcg ggcatgtatt gtctgatgct
     1381 gctggtgttc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcgggcgg
     1501 gctgctgttt gccgcatcga cagacggtgt gtcgtggacg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtcatgacc gaggcttacg gcgcggtatg
     1621 gggcagcatc atgctgctga acgatttggc acgagagctg tttgcactgg catttatccc
     1681 gctgctgatg aagcgttttc cggatgcggc ggtgggggtt ggcggtgcga ccagtatgga
     1741 ttttacattg cccgtgattc agggtgcggg cggtttggaa gtcgtgccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata tcgccgcccc gtttctgatg gtggtgtttt ccgctttggg
     1861 ttgaacgcgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgagttgga
     2041 ggaggccggc agcctgaaac gccatcacgg cggcgcatct tcgggcggaa acttgccgga
     2101 gggcctgccc gccgaccgcc aaacccggtg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa cacatccccg acggttcgtc gctgtttgtc agtatcggca cgacgatgga
     2221 agccgtggca tcggagctgg tcaggcggcg cggcagcctg cggattatta ccaacaatat
     2281 ccacgtcgcc tccatcgttt cggcgcggac ggattacacg gtcattatca cttccggagt
     2341 cgtccgccct ttggacggcg gtattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaatgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgcc tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat ttgcggacaa
     2821 ccattttatc cgcaccttca tcgaagaaga cctcaaaagc ggcaaacacg aaaccatcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggcgt atatttacga cggcttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagacg ttgagtggct
     3061 cggtttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttggtta aagacggcaa agcgtatgtc gatgatttga cgccagaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcaggcaaa aacagccctt atcgcgaccg
     3241 cagtatcgaa gaaaacctcg acctgttcac gcgcatgaaa aacggtgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatccggc aacatcaata tgcgcgatcc
     3361 ggtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgcatcta
     3421 tccgatgtac gactacacgc attgtatttc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctc gaatttgaag cccatcgtcc gctgtatgac tgggtgttgg acaacatccc
     3541 cgcgccgcac gccacacgtc cgcgccaata cgagttttca cgcttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacca tttccggtat gcgccgacgc ggctacacgc ccgaagggct
     3721 gcgcctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaattccgcc ccacgcctga tggcggtgtt
     3841 gaacccgctc aaagtgaccc tgaccaactt tgaaaccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aggaaatggg cgagcgcgaa gtacctattt cacaaaccat
     3961 ctatattgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtggttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tccagaaggc cgtaaagtta aaggcgtgat tcattgggtt tccgccgaac acgcagccga
     4201 aatcaaagtc cgcctgtacg accgcctctt taccgtcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagatggc gaatacctgc cgtttaccga tttcctcaat ccggaatccg ttaaggaaat
     4321 cactgcttac gccgaacctg ccgcgaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgcaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ctgccgtctg
     4501 aatattgttc ggacggcatt tctcctttac ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctat
     4621 aaagacacca cctacgcgat gatgcgcgag atggcaaaac gcggctggca gctgtttcat
     4681 accttgagca gcggattgtc ggttaaaaac ggtctggtgg ttgctgatgc gtctgctttt
     4741 gagtttctcg gcgcacaaga cgaccacgac aaaaactggt ttgccgccgc cgacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccacaca gcttctgacg ctggcggagc agcagggcgc gaaggtcttt
     4921 aacagcggac aggcgatgcg tgatttcaat gaaaaactgg cgattttgaa tttcagccgc
     4981 tttaccgcgc ccacgctggt aacgacccgt tccgccgatg tccgcgcatt tttgaaagaa
     5041 cacggcgaca tcatcattaa accgctcgac ggcatgggcg gtatgggcat cttccgcctg
     5101 accgaaaaag accccaacat cggcagcatc ctcgaaaccc tgatgcggct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtatcc cgcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg gcgcggcgtg gcgcaggaat tgggcggacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgcttgc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatt
     5461 atgaaacaaa aaggtttcga cgtggcggca atgtttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgtcgt ctgaaaggct tttgcttcac aaccgtttgg tttgatcggg
     5581 caggcagggt tttccctcag ccggcagaga tacgcttaat gccgtacgga caccccgccg
     5641 ccccgttttc agacggtata tatagtggat taacaaaaat caggacaagg cg
//
LOCUS       DAWFAN010000127         5652 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWFAN010000127 DAWFAN010000000
VERSION     DAWFAN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.127"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(129..890)
                     /locus_tag="WGO65_001600"
     CDS             complement(129..890)
                     /locus_tag="WGO65_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327879.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            990..1241
                     /locus_tag="WGO65_001601"
     CDS             990..1241
                     /locus_tag="WGO65_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327880.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEVA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1293..2099
                     /locus_tag="WGO65_001602"
     CDS             1293..2099
                     /locus_tag="WGO65_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ0327881.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2406..3929
                     /locus_tag="WGO65_001603"
     CDS             2406..3929
                     /locus_tag="WGO65_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ0327882.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            4027..5439
                     /gene="trkA"
                     /locus_tag="WGO65_001604"
     CDS             4027..5439
                     /gene="trkA"
                     /locus_tag="WGO65_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ0327883.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1415 a         1704 c         1369 g         1164 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttaaatt taatccacta tattaatatt ttgcgcctgt
       61 tctatgatgc tttcaagtcg ggtgagaatg caaatgccgt ctgaaacggc tttcagacgg
      121 catggcaatc agcgtttgta ttttaactcg tacttgatgt cgttgaggat tttgcggaca
      181 tcgtgttcca acacgtcttc gactaccgca cccgcctgct cgtgcagcat ctgctggagc
      241 tgataggtaa aaaccgccat ctgcttttgc accgccgtac ggatgatgcc gttgacggta
      301 tcggtcagat gcgggcgcag gcgtttgatc agccgttcgg tcagctcctg ttcggacagg
      361 cagaacactt cgcgccggtt gacggctttc ggattcagga tattgatttg gacgggcatc
      421 aacgcctctt ccgcaccgcc ttcgccgttt tccgcaaccc cgtctgccgc cgtacccgat
      481 tctgccgcat cggcgttttc cccgccttca atctgtccgg tttcaaattc ggcactgtct
      541 tttttggcat cgaacaggat tctccgccgc gattcgatgt gtttttccga aaccgacatt
      601 tgcagggaag cctgcgcgtt gagccagttt tcctgaagga caatcatcgg gtcggtttcg
      661 acttcctcgc cgcaatcggc aacggcggca ttgtgttcct cctgccattt tttcagatac
      721 gccttcaaca cacgggctcg gctctcatcg tccagtttcg gcacaggcgc gtccgttccg
      781 gtttcagagg ggcgggacag cggcgcgtaa gtcggcactg ccttcatacg gcgcgtctga
      841 cgcaggtttt ccaaacgttt ttcccaattc ggctctttat tcgcatccat tttcggcttc
      901 cggtccttaa tctttgcaag cagacaaacc cgcgcccaaa gcgcggtttg atataatggc
      961 gcattttaac agattcgcga ggatacatca tgggcagcat cgaacagcgt ttggaatatc
     1021 tggaagaggc gaacgacgtg ctgcgtatgc agaaccacgt cctgtccacc gccttcaaag
     1081 ccttaatccg cgcccttccc gcagacaccg ccgaagtcgc ggtcgagtcg attcagcttg
     1141 catttgaaga cgcactggca gaattgagct atgaggacag cccgcatacg gatttgttcc
     1201 acgacgttac ttatgcgttt ttccgtgaaa aggaacgtta attttatgtt aaactgattt
     1261 tttaggcttt ttgattaccg aaaggaattt tgatgaatat gaaaaaatgg attgccgccg
     1321 cccttgcctg ttccgcgctc gcgctgtctg cctgcggcgg tcagggcaaa gatgccgccg
     1381 cacctgccgc aaaccccgac aaagtgtacc gcgtggcttc caacgccgag tttgccccct
     1441 ttgaatcttt agactcgaaa ggcaatgtcg aaggtttcga tgtggatttg atgaacgcga
     1501 tggcgaaggc gggcaatttt aaaatcgaat tcaaacacca gccgtgggac agccttttcc
     1561 ccgccttgaa caacggcgat gcggacgttg tgatgtcggg cgtaaccatt accgacgacc
     1621 gcaaacagtc tatggacttc agcgacccgt attttgaaat cacccaagtc gtcctcgttc
     1681 cgaaaggcaa aaaaatatct tcttccgacg atttgaaaaa catgaacaaa gtcggcgtgg
     1741 taaccggcta cacgggcgat ttctccgtat ccaaactctt gggcaacgac aacccaaaaa
     1801 tcgcgcgctt tgaaaacgtt cccctgatta tcaaagaact ggaaaacggc ggcttagatt
     1861 ccgtagtcag cgacagcgcg gtcatcgcca attatgtgaa aaacaatccg accaaaggga
     1921 tggacttcgt taccctgccc gacttcacca ccgaacacta cggcatcgcg gtacgcaaag
     1981 gcgacgaagc aaccgtcaaa atgctgaacg atgcgttgga aaaagtacgc gaaagcggcg
     2041 aatacgacaa gatttacgcc aaatattttg caaaagaaga cggacaggcc gcaaaataag
     2101 cccgcccgtc cgaacacaat gccgtctgaa gccctttcag acggcattgt tcatcaatcg
     2161 gcctacaatg aactgcctgc tgatttctcc ctaccgcaaa gcaacaggca aagattacaa
     2221 atatcaaaat ccgagtaaaa cagtatttta ttaaaacaaa ttgataatca agagattaga
     2281 attatgtatt gtctttaccg tacaaacgct ggcactattt caacctgata aaaaacagcc
     2341 ttcaaaaagg ttgtttaaaa cagcagcaga cacttaccgc cacaaccttg aaaaggaaca
     2401 caatcatgac cgtcatcaaa caggaagact ttatccaaag catttgcgat gccttccaat
     2461 tcatcagcta ctaccatccc aaagactaca tcgacgcgct ctataaagcg tggcagaagg
     2521 aagaaaaccc cgccgccaaa gacgcgatga cgcagatttt ggtcaacagc cgtatgtgtg
     2581 cggaaaacaa ccgccccatc tgccaagata ccggtatcgc aaccgtgttt ttgaaagtcg
     2641 gtatggatgt gcagtgggat gcggatatga gcgtggaaga aatggttaac gaaggcgtgc
     2701 gccgcgccta cacttgggaa ggcaatacgc tgcgcgcttc cgttctcgcc gaccccgccg
     2761 gcaaacgcca aaataccaaa gacaacacgc ccgccgtcat ccatatgagc atcgtgccgg
     2821 gcgacaaagt cgaagtaacc tgcgcggcaa aaggcggcgg ctctgaaaac aaatccaaac
     2881 tcgccatgct caatccttcc gacaacatcg tcgattgggt attgaaaacc atcccgacca
     2941 tgggcgcggg ctggtgtcct cccggcatct tgggcatcgg catcggcggc acgcccgaaa
     3001 aagccgtgtt gatggcaaaa gaatccttga tgagccacat cgacattcaa gagttgcagg
     3061 aaaaagcagc gtcaggtgca gaattgtcca ccaccgaagc cctgcgcctc gaactctttg
     3121 aaaaagtcaa cgcgctgggc atcggcgcgc aaggcttggg cggtctgacc accgtgttgg
     3181 acgtgaaaat cctcgattac ccgacccacg ccgcctccaa accgattgcc atgattccga
     3241 actgcgccgc cacccgccac gtcgaatttg aattggacgg ctcaggccct gtcgaactca
     3301 cgccgccgcg cgtcgaagac tggcccgatt tgacttacag ccccgacaac ggcaaacgcg
     3361 tcgatgtcga caagctgacc aaagaagaag tggcaagctg gaaaaccggc gacgtattgc
     3421 tgttgaacgg caaaatcctc accggccgcg atgccgcaca caaacgcctc gtcgatatgc
     3481 tcaacaaagg cgaagaattg cccgtcgatt tcaccaaccg cctgatttac tacgtcggcc
     3541 ccgtcgatcc ggtcggcgat gaagtcgtcg gtccggcagg tccgaccaca gccacccgca
     3601 tggacaaatt cacccgccaa atgctcgaac aaaccgacct cttgggcatg atcggcaaat
     3661 ccgagcgcgg cgtggccacc tgcgaagcca tcgccgacaa caaagccgtg tacctcatgg
     3721 cagtcggcgg cgcggcgtat ctcgtggcaa aagccatcaa atcttccaaa gtcttggcgt
     3781 tccccgaatt gggcatggaa gccatttacg aatttgaagt caaagacatg cccgtaaccg
     3841 tcgccgtaga cagcaaaggc gaatccatcc acgccaccgc cccgccccaa tggcaggcga
     3901 aaatcggcat catccccgtc aaatcttgag gcgtcatgcc gtctgaacac aaaatctgct
     3961 ttcagacggc atttccgccc ctggttgcgg tacaatccac catttcatca ctcggcgacc
     4021 cacaccgtga aaatcctcat tttaggcaac ggacaggtag gttctaccgt cgcacaaaac
     4081 cttgccgcca tacccaacaa cgacgtaacc gttatcgaca tcgacgaaaa agcattgcag
     4141 gaaacaggca gccgcctcga tgtccaaacc gttttcggca acggcgcatc ccccttcaca
     4201 ttagaacgcg ccggcgcgga agatgccgac ttgctgctcg cgctctcccg cagcgacgaa
     4261 accaacatcg tcgcctgcaa agttgccgcc gacctgttca acatccccgg ccgcatcgcg
     4321 cgcgtccgtt ccagcgaata cctcgaatac ctcagcccca agctcgaaaa caacgaaaac
     4381 ggcagccttt ccatattcgg cataaccgaa accatcagcc ccgaacagct cgttaccgaa
     4441 cagcttgccg gcctgataga ctgcccgggc gcattgcagg ttttacgttt tgcagacgac
     4501 cgcgtgcgga tggtcatcat acaggcgcgg cgcggcggac tgcttgtcgg acgcagcatt
     4561 gccgacatcg cccaagattt gcccgacggg gccgactgcc aaatctgcgc cgtttaccgc
     4621 aacaaccgcc tcatcgtccc cgcgccgcaa accgtcatca tcgaaggcga cgaaatccta
     4681 tttgccgccg ccgccgaaaa catcggcgcg gtcatacccg aattgcgccc caaagaaacc
     4741 agcacccgcc gcatcatgat tgccggcggc ggcaacatcg gctaccgtct cgccaagcag
     4801 ctcgaacacg catacaacgt caaaatcatc gaatgccggc cgcgccgtgc cgaatggata
     4861 gccgaaaacc tcgacaacac cctcgtcctg caaggttcgg caaccgacga aaccctgctc
     4921 gacaacgaat acatcgacga aatcgacgta ttctgcgccc tgaccaacga cgacgaaagc
     4981 aacattatgt ccgccctttt ggcgaaaaac ctcggcgcga agcgcgtcat cggcatcgtc
     5041 aaccgctcaa gctacgtcga tttgctcgaa ggcaacaaaa tcgacatcgt cgtctccccc
     5101 cacctcatca ccatcggctc gatactcgcc cacatccggc gcggcgacat cgttgccgtc
     5161 caccccatcc ggcgcggcac ggcggaagcc atcgaagtcg tcgcacacgg cgacaaaaaa
     5221 acttccgcca tcatcggcag gcgcatcagc ggcatcaaat ggcccgaagg ctgccacatt
     5281 gccgccgtcg tccgcgccgg aaccggcgaa accattatgg gacaccatac cgaaaccgtc
     5341 atccaagacg gcgaccacat catctttttc gtctcgcgcc ggcgcatcct gaacgaactg
     5401 gaaaaactca tccaagtcaa aatgggcttt ttcggataaa ccgccccatt ccggacacat
     5461 tgccgccaag cggtatggaa gcggaataat ggtaggtggg cttcagacgg catcgtcccc
     5521 caccgttatt cccgcgcagg cgggaatccg gtctgttcgg tttcagttgt ttttgggttt
     5581 cgggtaattt ccaaatcgtc attcccgcac aggcgggaat ccaaaccatt ggacagcggc
     5641 aatattcaaa gg
//
LOCUS       DAWFAN010000128         5630 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWFAN010000128 DAWFAN010000000
VERSION     DAWFAN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.128"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(127..2259)
                     /locus_tag="WGO65_001605"
     CDS             complement(127..2259)
                     /locus_tag="WGO65_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ0327884.1"
                     /translation="MSIASAPLPALSALIILAHYHGIAANPADIQHEFCASAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPVKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            complement(3024..3707)
                     /locus_tag="WGO65_001606"
                     /pseudo
     CDS             complement(3024..3707)
                     /locus_tag="WGO65_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            4044..4478
                     /locus_tag="WGO65_001607"
     CDS             4044..4478
                     /locus_tag="WGO65_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327885.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            4754..5473
                     /locus_tag="WGO65_001608"
     CDS             4754..5473
                     /locus_tag="WGO65_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327886.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
BASE COUNT         1720 a         1214 c         1145 g         1551 t
ORIGIN      
        1 aagacagaaa aagtctttcc ggcagcctgc actttggttt catttcagtc agtaaaccca
       61 gtaaacgacg gtctgaaaac gcagaacgtt acgaaaaaag cagcctacac gcccatcccc
      121 cgccttctac ccgttctgta aatcatacag atagcggtaa tatccgttcg gcttcgccag
      181 caattcctgc tgtgttcccg cttccacaat cctgccttta tccatggcaa tgatccggtg
      241 tgccgtttta acagtggaca gacggtgggc gataatcagc accgtccggt tggcgcaaat
      301 ggcctgcatg ttctgcataa tcgctcgttc actttcataa tccagcgcgc tggtggcttc
      361 atcaaaaatc agaatgcgcg gattggtgat taacgcgcgg gcaatcgcaa tacgctgccg
      421 ctgtccgccc gacaagccgg ccccttgttc gcccaccacg gtgccgtagc cttccggcag
      481 ctccataata aactcgtgtg cgcccgccag tttggctgct tcgataatgc gttccagcgg
      541 catacccgta tccgtcagcg cgatattgtc gcgtatgctg cggttgagca gcacattctc
      601 ctgcaagacc acgccgacct gccgccgcag ccaagcagga gcggccaaag ccaaatcgtt
      661 gccgtccacc aacacccgtc cctgctccgg tacatacaga cgctgcacca atttggtgag
      721 tgtggatttg cccgaccccg aacgtcccac aatccccagc acttcccccg cccgaatccg
      781 caggttcaaa tcctgcaaaa tcagcctgcc gtccgcctta tagcggaaat cgacatgttc
      841 gaacgtaatc tccccccgga tatcgggcaa agccaaatgc gaagacgcat tctcggtcgg
      901 cgcattcaga atatccccca aacgcgccac cgaaatcccc acctgctgga aatcctgcca
      961 caactgcgcc aaacggataa caggcgccgc cacctgtccc gagagcatat taaacgcaat
     1021 cagctgcccc accgtcagct tgccctcaat taccagccgt gcgccaatcc acaacgtcgc
     1081 caccgtcacc agcttctgaa tcagctgcac cccctgctgg ccgaccaccg ccaacttcgt
     1141 tacccgaaat cccgaagcca cataagccgc caactgattg tcccaacgct gcgtcatctg
     1201 cggctccacc gccatcgcct ttaccgtacc caccgcagtg atgctttcta ctaaaaacga
     1261 ctggttgtct gcattgcgcg cgaacttatc gttcagacgc gtccgcagta tcggactgat
     1321 aaatgccgac caaaacgcat aggcaggcaa cgaagccaat accacccaag tcagagtgga
     1381 gctgtaatac cacatcaccg ccagaaagat aaacgaaaac gccaaatcca acaccgaagt
     1441 cagcgcctga ccggtcaaga aattgcgaat ctgctccaat tcccgcaccc gagccaccgt
     1501 atcacccact cgtctgtgct cgaaatagga taaaggcagg gaaagcagat gccggaacaa
     1561 acgcgcgccc aattccacat caatacgtga agtcgtatgt gcaaacagat acgtccgcaa
     1621 accgcccaac acaatctcaa acagcgacac caccaacaaa gccaccgaca ccacatccaa
     1681 agtagagaat ccccgatgta ccagcacctt gtccatcacc acttggaaaa acagaggcgt
     1741 aatcagcgca aacagctgca acaccaccga caccaccaat acttcaaaaa acaaccggcg
     1801 gtatttgatt accgccggaa taaaccaggt aaagtcaaac tttgccaaac tgcccaatac
     1861 cgaagcgcgg gaagcaacca atatcagttt gcccgaatat ctgttagaaa attcggcaaa
     1921 agacaatacc gcagacttat tcgtagttaa atcctgtatt agatattggg catgctcacc
     1981 cccaccgtct gttttagcca aaataaaatg gttgccgtca tcacaccata ccaatgcagg
     2041 taaggtcgcc atagccaaac gtttgacagg ctggcggact acctttgcct tcaatcccaa
     2101 agatttggcg gccaaaagcc attgtgtttc atttaaatcg ctttgtgcgg aagcacaaaa
     2161 ttcatgttgt atatcagcag gattggcggc aatgccgtgg taatgggcga ggatgatgag
     2221 ggcggaaagg gcggggagcg gtgcggatgc gatagacata aataaaatat agttagattg
     2281 gatgtggata acggctggct ggaaaaggaa tatattaagt agaaagaaat atataaataa
     2341 aacagcagaa cgcattgtaa ggatatatat gggaattgta aagagaaagt atggaaaagt
     2401 tctcgtttca ggaaggtaaa acggcttagg aatcgagtta gatgaggatg cctcgcacct
     2461 ctcgtgcctc ctgcataccg ttaaggcaca gggttaaggt gcaggctgct ccgaactctg
     2521 ttgcggtcgg gtaatgttat tttttgtgtt tcaggcagcc tgaaatatct gtatattttt
     2581 gttttaaata gattttaaag attgataact gttcttgacg atttttcaag aaaggagtaa
     2641 atttcaagaa aggagtaaag tgacttatta tcaatgacaa gcaacgcgcg aagtgacaag
     2701 gaaaactatc tacttaaatt ctaaggaggc ttcgaatatc ataaaccaat cagaaacata
     2761 gagataaaaa ttatgtacaa atataatcct cttatacaat ttattgcaca gttgattatg
     2821 tcttatggag caagcgtagg gtgggcactt gctgccccac gcgtttcata tttcaaggca
     2881 gcctgaaacc gtgtgggcat aaatgcctac cctacatccc aaaaaacaag cgcagcctgc
     2941 gtgtgtaggg tgcgaacttt cggcaggtag acacgcagtt ttatattttc aagctgaggg
     3001 atgcttaaga aaagtacaaa acattaaaaa ataaggggct gtactagatt agccctaaat
     3061 ccacaccaat cccgcaagat ttttagctgt cgggacggtg tgccgaagtt aaatcgaaat
     3121 tcgcattctt tcaagaacag cgggaaagat ttgcgatcaa ttccgttcta tttgcgcaag
     3181 acgcgttttg cctgattcca aaagttctca atgccgttta tgtggttctg acggtcagca
     3241 aattccttgg aatggttgat gcggtaatgg ataaaaccgc ttacgtccaa cttgtcgtaa
     3301 ctgctcaggc tgtcggtata aacaatgctg tccggcatga ttttcctttt gatgacaggg
     3361 agtaacgttt cagacctggc attatctaca acaacggtat agacccgtcc gttgcgtttc
     3421 agaatgccga agacaaccac ttttcctgcc gcaccgcgac cgcgtctgcc tttacgccgt
     3481 ccgccgaaat agctttcgtc caactcgaca gagccctcaa aaacctcatc ggcagccaag
     3541 gccggataat ggctgatgac catacggatt gtgcggtaga acagaatagc ggagtcgggc
     3601 tggatgccca aaatatcggc agctgaacgg gcggtaactt caagtacaaa aaagcggagc
     3661 agttctttct gtactttttt ctttaatttg cagtgtgttg tcttcatatt tcgagggtaa
     3721 catatctgct aatctagtac agcctcaaac aaaaaagaga aattttaatt tcgctaaatc
     3781 gcataaagat taatcaagag tatcattaaa tgatatgagt gagcatctat aatgccaaga
     3841 agagttgtta agacataatg attattgaaa tagattgtaa aatagatact tagatagtct
     3901 gaaaaacgga tttgtgaaac tttttattac gcgccatcat ttgaaaatga aacttaaaaa
     3961 acacttatca taataaatat tttctttacg ttgtttgcta ataaactcag tgcaatatca
     4021 tcgcaatatt ttatggaaat tttatggata acaaaaaaga atttattaat aatttaacaa
     4081 ataggtatat gtggatctat ccattggtct taaatattct atttctacct ttttaccagt
     4141 cctaccaatc tttttttatt gcgcttggtt gtttgtttgc actggttaga aaaatgcaaa
     4201 gcttagattt taaattacaa aatcatattg tattgttaaa tataaaaagt gcttgggcag
     4261 ataaaaaagt atttttgatt aggatagtag tgtcatggtt ggcagtaatg gaaatatgga
     4321 tgtgttttat ttcggaatca tcaacgtggg tatgcggtgc tttttgttta aatagtgaaa
     4381 tattggaaaa aatttttcgt ggctttggtt attctggtag tttatatttt ttatttatat
     4441 tgatgattga tctcaataag ttaagagaga gtatttgaat ttcatttttt gtttgactta
     4501 aactcaagga gagtaacaat gattggtagt ggtgatacta aacaatgcaa aaaattttct
     4561 gcgtgtgatg gaaaatacca cgtctacgat cccctcgccc tagacttgga cggcgacggc
     4621 atagaaacag tcaccgccaa aggcttttca ggcagcctga agactgagag agtgaatacg
     4681 atgagtatac actctatgcc actaaattga tattcactaa atcataccag ctatatttta
     4741 tttaatgaga catatgaaaa ataaaaatta tttactagta tttatagttt tacatatagc
     4801 cttgatagta attaatatag tgtttggtta ttttgttttt ctatttgatt tttttgcgtt
     4861 tttgtttttt gcaaacgtct ttcttgctgt aaatttatta tttttagaaa aaaacataaa
     4921 aaacaaatta ttgtttttat tgccgatttc tattattata tggatggtaa ttcatattag
     4981 tatgataaat ataaaatttt ataaatttga gcatcaaata aaggaacaaa atatatcctc
     5041 gattactggg gtgataaaac cacatgatag ttataattat gtttatgact caaatggata
     5101 tgctaaatta aaagataatc atagatatgg tagggtaatt agagaaacac cttatattga
     5161 tgtagttgca tctgatgtta aaaataaatc cataagatta agcttggttt gtggtattca
     5221 ttcatatgct ccatgtgcca attttataaa atttgcaaaa aaacctgtta aaatttattt
     5281 ttataatcaa cctcaaggag attttataga taatgtaata tttgaaatta atgatggaaa
     5341 caaaagtttg tacttgttag ataagtataa aacatttttt cttattgaaa acagtgtttg
     5401 tatcgtatta attattttat atttaaaatt taatttgctt ttatatagga cttacttcaa
     5461 tgagttggaa tagttttggt aattttatga gcgcacgctc atccgcgtta gcagaatttg
     5521 gaaatatggt tgctaattta gtttctacaa aaaatgagaa agatatctcg aaacgtaatg
     5581 aatattacaa acaagctggt tatagtgcat tattagcatt tggtaatttg
//
LOCUS       DAWFAN010000129         5624 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWFAN010000129 DAWFAN010000000
VERSION     DAWFAN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.129"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(173..328)
                     /gene="rpmG"
                     /locus_tag="WGO65_001609"
     CDS             complement(173..328)
                     /gene="rpmG"
                     /locus_tag="WGO65_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ0327887.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(360..593)
                     /gene="rpmB"
                     /locus_tag="WGO65_001610"
     CDS             complement(360..593)
                     /gene="rpmB"
                     /locus_tag="WGO65_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ0327888.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(843..2369)
                     /gene="farB"
                     /locus_tag="WGO65_001611"
     CDS             complement(843..2369)
                     /gene="farB"
                     /locus_tag="WGO65_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ0327889.1"
                     /translation="MDYPPLKGAALAWITLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     ALVIFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(2393..3565)
                     /gene="farA"
                     /locus_tag="WGO65_001612"
     CDS             complement(2393..3565)
                     /gene="farA"
                     /locus_tag="WGO65_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ0327890.1"
                     /translation="MATRGDEVKIQNGQEADSAKAKRKRRLTALTLLFALAAVAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     gene            complement(3609..3968)
                     /locus_tag="WGO65_001613"
     CDS             complement(3609..3968)
                     /locus_tag="WGO65_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0327891.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     regulatory      4183..4227
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            4232..4705
                     /gene="queF"
                     /locus_tag="WGO65_001614"
     CDS             4232..4705
                     /gene="queF"
                     /locus_tag="WGO65_001614"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ0327892.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFETLARKRLFEHDSQ"
     gene            4780..5466
                     /locus_tag="WGO65_001615"
     CDS             4780..5466
                     /locus_tag="WGO65_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ0327893.1"
                     /translation="MYAFTAAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT         1424 a         1581 c         1266 g         1353 t
ORIGIN      
        1 aaatagtacg gaaccgattc acttggtgct tcagcacctt agagaatcgt tctctatcct
       61 tggcagacgg tttgcagcag attcgcatac ggcagatgtt tcaagcggaa aagaaacacg
      121 ataaaaataa aaaagcctcc gaacagtcgg aggctttgct ttcaaactga aattatttca
      181 gtttggtttc tttgtacact acgtgtttgc gggcaaccgg atcgaatttt ttaatttcca
      241 gtttgccggg catagtgcgt ttgtttttgg tagtggtgta gaagtgacca gtacctgcac
      301 tggattccag tttgatttta tcgcgcattg cagtaatcct taattaaata gtgtttaaat
      361 taagcttcgc cgcgagcacg caaatcagcc aatacgacat caatgcctac tttgtcgatg
      421 gtacgcagtg cagcgttgga aacgcgcagg cgaacccagc ggttttcact ttctacccaa
      481 aaacgacgtg attgcaagtt gggcaaaaaa cggcgtttgg ttttgttgtt ggcgtgcgat
      541 acgttgttgc cggacatcgg gcgtttgccg gtcactttgc aaactcgtgc cataatcagt
      601 ctccaaactt ctaaaatagt aaaacgcgat ttataccacg aaaaacagtg ttagttcaag
      661 tgttttaaag aaaaacggct gttttgcgcg gataatcctt ccgattgtcg catctgtgga
      721 acgtttgcag gtgcgaaaca gatggggcgc gattatagcg gatttcggga actgctgcat
      781 tcaaaatgcc gtccgaacat atttccagta ttttcagacg gcatttcaag ttttcaaatc
      841 cctcaatgtc caccgccgcc gccgttgtgg aacggcggtt ttgccagcca tatgacgggt
      901 atcatgataa tgaataacaa gctgcccgcc ataaagattt cgttcgagcc gataataaaa
      961 ccctgctggg taatggtatt gttgatgatg cctagggttt gaatgtcgga aacgccgtgc
     1021 tgggacaaat gagcggccgt ttcgtgcaat gttgcggaat agggcgtgat gtgttcggca
     1081 aagcgtgtat ggtgcaacgc ttcgcgccgt tcccacaggg tgctgacgac ggatacgccg
     1141 acaccgccca tcagcacgcg caagaaattc gacaggctgc ctgcggcggc aatctgtccg
     1201 cccttcatgt gcgacagtgt gatggtggtc agcggcagga agaacatggc aacgcctgcc
     1261 ccctgccaaa actgcggcca gatgacgttg ccgatatcca tgtcggcata aaaatcggtg
     1321 cgccaataga aagtaaaggc aaaggtcagg aagctggcgg ttacgagcag gcgcatatcg
     1381 attttattgc cgaacctgcc gattaacgga gacaggaaga cgggcaggat gccgacgggt
     1441 gcggcggcaa gccccgccca cgtggaggta tagcccaggt tggtctgcaa cactaacggc
     1501 agcaaggtca gcgtccccat atacaccata aaacccaatg atgtggcaat tacgccgacg
     1561 gtaaaattcc gatctttaaa cagcgataaa tcgacaatcg gatatttttc tcccaattcc
     1621 caaacaataa aatacgacaa acacaccagt gcgactacgc ccaaggtaat gatttctcca
     1681 gaggcgaacc agtcgagttc cttacccctg tccagcatca tctgtaacgc gccgataccg
     1741 actaccatca atgtaagccc gacatagtcg gtcggcattt taacggtttc cgtttcccga
     1801 tatttcaaat gtttccatgt aatccatgcc gatatgatac cgatagggat attaatgaag
     1861 aaaatccaac cccaatgcca gtttccggaa atccaaccgc cgagtatcgg cccgagaaca
     1921 ggggcgacaa cgacggtcat tgcccacaat gccagtgcca gcgtccgttt tgcgggcgga
     1981 taggatgcca ttaacaggct ttgcgacagg ggaatcagcg gcccggcgat aaagccctgc
     2041 aagatgcgga aaattaccaa agcctgaagg ttgggcgcga tgccgcacag ccacgatgtg
     2101 atgacgaaac cgacagcggc ggcggtaaac aatttgacct cgccgatacg ttttgccaaa
     2161 aagcccgtta acggcacgga aacggcgttt gccacagcaa aagaagtaat gacccacgtc
     2221 ccctgagtgg ttgccgcacc gaggttgccg gcgataacgg gaacggcgac attggcgata
     2281 gtcgtatcta aaacttccat aaatacggca agccccaaag acagcgtaat ccatgccaat
     2341 gccgcaccct taagcggtgg ataatccatt gtgtcctccg tttcagacgg catcagggcg
     2401 cggattgccc gaggatttca tcgaccgtcc gatcgacttc cgaccagtcg gtactttcca
     2461 tttccggcaa tgccgcaccc ggcgtttttg aaacaggcgc gcctgcggcg gaagtatcca
     2521 ctttaaccgt catcgacaaa ccgatacgca acggatgcct gtccacatct tcgcggttca
     2581 gcacgatacg gacggggacg cgctgcacca ctttaatcca gttgcccgtt gcgttttgcg
     2641 ccggaatcag cgaaaacgcg ctgcccgtac ctgccgaaaa acctgccacc ctgccgcgat
     2701 aaacaatttg tttgccgtac aaatcggaca ccagctcggc aggctgtccg attttcatat
     2761 gccgcaactg cgtctcttta aaattggcat ccacccacac atccgacagc ggcaccaccg
     2821 ccatcagcgg cgcgcctgcc gccacctgct gcccgacctg caccgaacgc ttcgccacct
     2881 gaccgtccgc cggcgcgcgg atttgcgtcc gctgaaggtt caaccacgca tctttcaacc
     2941 tgccgattgc cgtctgaacc gccggctgtt cgcgcaaaga aacctgaccg cccaaagccg
     3001 cgcgtgccga agactcttcc gccaaagccg ccttaactgc cgcctgcgcc tgagacactg
     3061 ccgcacgggc gtgtgccagc tcttcggcgg acaccgcgcc cgattccgcc aaagcagacc
     3121 ggcggcgcaa atcatcctgc gcgcgtgcca aatccgcacg gcgcaaggca acctgcgccc
     3181 ccgcctgcga agtggcggca ttttgccggc ggttttgccg caccgcctga accagctcgt
     3241 tttttgcccg ctcgtaagcc agcacatcat tatcgtcgtc caataccgcc agcacgtcgc
     3301 cttttttcac ggcatccgta tcgtcgtgca aaaccttccg taccgtaccg cccttttgcg
     3361 gcgtaacctg aaccacgcgt ccggcaacat aagcgtcttc cgtttcctct tcgtgctgcc
     3421 accataaaaa aaacgccgac ccagcggcta cggcggcaag cgcgaacagc agcgtcaatg
     3481 ccgtcaggcg gcgtttgcgt ttggcttttg cgctatcggc ttcttgcccg ttttgaattt
     3541 tcacttcgtc tccgcgtgtt gccattattc caattccgaa tacttctaat actatgtttt
     3601 tgatttaatt agcactgata atcttcaaat tcccacgaaa aaaaacgaag taaataagtc
     3661 aatgactttt cccaagtttc ttttgaacat tctttaagaa ttttctcaat ttccgattta
     3721 ataacagaat gattaaattc attcataatc atcatacccg ctccccattt aaccctttga
     3781 ttttggaaac aattatgcaa aatccattta ggagagcata tgcgaacaga aaatatatct
     3841 gcagcatcac tatcatcagt tcctatgtct aaatcaattc ccacacaaaa attgtctttg
     3901 atttcgggaa cgaaatcttc aaaggcacaa tcgtaaagat tgatggcttt caattctagg
     3961 ttaatcattt tatattcaaa ataaataaaa ccaattaaag attggttagg attctatacc
     4021 ctggcaataa ctcaatcaag ttaatgattt agaatgaata ttcaaataaa ctgtatcagc
     4081 ccggcctcac agaactattt atatccacac atcaccgagt aaagcaatta cccatactaa
     4141 ccgaagaaaa ctacacaaat accgccactt atattacaat cgccgctcgt ggttcgaaaa
     4201 cctcccacat taaaaaacta aggaaaccct catgtcccgc aacaacgaag agctgcaagg
     4261 catctctctt ttgggcaatc aaaaaaccca atatccgacc ggctacgcgc ctgaaattct
     4321 cgaagcattc gacaacaaac atcccgacaa cgactatttc gtcaaattcg tctgtcccga
     4381 gttcaccagc ctctgcccca tgaccgggca gcccgacttc gccaccatct acatccgcta
     4441 cattccgcac atcaaaatgg tggaaagcaa atccctgaaa ctctacctct tcagcttccg
     4501 caaccacggc gattttcatg aagactgcgt caacatcatc atgaaagacc tcattgccct
     4561 gatggatccg aaatacatcg aagtattcgg cgagttcaca ccgcgcggcg gcatcgccat
     4621 tcatcctttc gccaattacg gcaaagcagg caccgagttc gaaacattgg cgcgtaaacg
     4681 cttgttcgaa cacgacagcc aataaaccat ccttcccata tttcagacgg cattccttcg
     4741 atacgatatg ccgtctgaac gcctttccga aagattttta tgtacgcatt tactgccgca
     4801 cagcaacaga aggcactctt ctggctggtg cttttccata tcctcatcat cgccgccagc
     4861 aactatctgg tgcagttccc cttccaaatt ttcggcatcc acaccacttg gggtgcgttt
     4921 tccttcccct tcatcttcct cgccaccgac ctgaccgtcc gcattttcgg ttcgcacttg
     4981 gcacggcgga ttatcttttg ggtgatgttc cccgcccttt tgctttccta cgtcttttcc
     5041 gttttgttcc acaacggcag ttggacaggc ttgggcgcac tatccgaatt caacaccttt
     5101 gtcggacgca tcgccttggc cagctttgcc gcctacgcgc tcggacaaat ccttgatatt
     5161 tttgtattca acaaattacg ccgtctgaaa gcgtggtgga ttgccccaac cgcatcaacc
     5221 gtcatcggca acgccttgga tacgttggta tttttcgccg ttgccttcta tgcaagcagc
     5281 gatgaattta tggcggcaaa ctggcagggc atcgcttttg tcgattacct gttcaaactc
     5341 accgtctgcg gtctgttttt cctgcccgcc tacggcgtga ttctgaatct gctgacgaaa
     5401 aaactgacga ccctgcaaac caaacaggcg caagaccgcc ccgcgccctc gctgcaaaat
     5461 ccgtaaaaca gccctgccgg gaatcgggac ggttgggttt tcttttattt gcaaacactg
     5521 tcggaacgga taaatccgga ttcctaccta cttcgtcatt cccgcgaaag tgggaatccg
     5581 gtgcgttggg tttccatcat ttccgataaa ctcctgccgc gttg
//
LOCUS       DAWFAN010000130         5579 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWFAN010000130 DAWFAN010000000
VERSION     DAWFAN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.130"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            80..1045
                     /locus_tag="WGO65_001616"
     CDS             80..1045
                     /locus_tag="WGO65_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0327894.1"
                     /translation="MLRLTALAVCTVLALGACSPQNSDSAPQAKEQAVSAAQSESASL
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            complement(1131..1238)
                     /locus_tag="WGO65_001617"
                     /pseudo
     CDS             complement(1131..1238)
                     /locus_tag="WGO65_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1379..2347
                     /locus_tag="WGO65_001618"
     CDS             1379..2347
                     /locus_tag="WGO65_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0327895.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLMGDKLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAYAV"
     gene            2337..3311
                     /locus_tag="WGO65_001619"
     CDS             2337..3311
                     /locus_tag="WGO65_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ0327896.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     QLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVYLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVKHSVRLP
                     MTVCIGGILLVGGQTVFEHLLGMQAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3355..3981
                     /locus_tag="WGO65_001620"
     CDS             3355..3981
                     /locus_tag="WGO65_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327897.1"
                     /translation="MTQEHFPSFFNQAPTITVQDALAEFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAFMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRHLLSFGVGEINGTLTLRRKDNGKTVAVGLNAALQPFAPE
                     MRDIMPKAVSGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            4064..4822
                     /locus_tag="WGO65_001621"
     CDS             4064..4822
                     /locus_tag="WGO65_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0327898.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLADTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPNDIFTAANIKTLFDMDVDVLDYEGKKLVIHHI"
     gene            5114..>5579
                     /locus_tag="WGO65_001622"
     CDS             5114..>5579
                     /locus_tag="WGO65_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NadA"
                     /protein_id="HEZ0327899.1"
                     /translation="MKHFPSKVLTTAILATFCSGALAATNDDDVKKAATVAIAAAYNN
                     GQEINGFKAGETIYDIDEDGTITKKDATAADVEADDFKGLGLKKVVTNLTKTVNENKQ
                     NVDAKVKAAESEIEKLTTKLADTDAALADTDAALDATTNALNKLGENITTFTE"
BASE COUNT         1275 a         1589 c         1434 g         1281 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat
       61 ccccattttg gagacaccta tgttacgttt gactgcttta gccgtatgca ccgtcctcgc
      121 cttgggcgcg tgttcgccgc aaaattccga ctctgcccca caagccaaag aacaggcagt
      181 ttccgccgca caatccgaaa gcgcgtccct gactgtcaaa acggcgcgcg gcgatgttca
      241 aataccgcaa aaccccgaac gtatcgccgt ttacgatttg ggtatgctcg acaccttgag
      301 caaactgggc gtgaaaaccg gtttgtccgt cgataaaaac cgcctgccgt atttagagga
      361 atatttcaaa acgacaaaac ctgccggcac gctgttcgag ccggattacg aaacgctcaa
      421 tgcctacaag ccgcagctca tcatcatcgg cagccgcgcc gccaaggcgt ttgacaaatt
      481 aaacgaaatc gcgccgacca tcgaaatgac cgccgatacc gccaacctca aagaaagtgc
      541 caaagagcgt atcgacgcgc tggcgcaaat cttcggcaaa caggcggaag ccgacaagct
      601 gaaggcggaa atcgatgctt cgtttgaagc ggcgaaaact gccgcacaag gcaaaggcaa
      661 aggtttggtg attttggtca acggcggcaa gatgtccgcc ttcggcccgt cttcacgact
      721 gggcggctgg ctgcacaaag acatcggttt gcccgctgtt gacgaagcca tcaaagaagg
      781 cagccacggt cagcctatca gctttgaata cctgaaagag aaaaatcccg actggctgtt
      841 tgtcctcgac cgaagcgcgg ccatcggcga agagggtcag gcggcgaaag acgtgttgga
      901 taatccgctg gttgccgaaa caaccgcttg gaaaaaagga caggtggttt accttgttcc
      961 tgaaacttat ctggcggccg gcggcgcgca agagctgctg aatgcaagca aacaggttgc
     1021 ggatgcgttc aacgccgcga aataatgaaa cggcggcatt cgatgccgtc tgaaacacgg
     1081 atgcaaaccg cttcccgtgt ttcagacggc attgcccgat acgggcgtta tagtggatta
     1141 aatttaaacc agtacggcgt tgcctcgcct tgccgtacta tctgtactgt ctgcggcttc
     1201 gtcgccttgt cctgatttaa atttaatcca ctatatcgcg ggaatggcgg caagcgtttt
     1261 tattggaact tgccctatcc tctgcccgat acggaggctt caaacaaggc tttccgctcc
     1321 gacggttcgg actgccttgt ttgaatcttc tacgccttaa cgcttttccc ttctgtttat
     1381 gactgccaaa cctttttccc tcaacctgac caacctcctg ctgctggcgg tgttgtttgc
     1441 cgtcagcctg tcggtcggcg ttgccgattt ccgctggtcg gatgtgtttt cgctgtcgga
     1501 cagccagcag gttatgttca tcagccgcct gccgcgcacg tttgcgattg tgctgacggg
     1561 cgcgtcgatg gcggtggcgg ggatgattat gcagattctg atgcgtaacc gttttgtcga
     1621 accttctatg gcgggcgcgg gtcagagtgc ggctttgggt ttgcttctga tgtccctgct
     1681 gctgcctgcc gcgccgctgc cggtcaaaat gtcggttgcc gccgttgccg cgttaatcgg
     1741 gatgctggtg tttatgctgc tgatccgccg tctgccgccg accgcgcaac tgatggtgcc
     1801 gctggtcggg attattttcg gcggcgtggt tgaggcggtg gcgaccttta tcgcgtatga
     1861 aaacgaaatg ctgcaaatgc tcggcgtgtg gcagcagggc gatttttcag gcgtactgct
     1921 ggggcggtac gagctgcttt ggattacggg cggtttggcg gtgtttgcct atctgattgc
     1981 cgaccggctg acgattttgg ggctgggcga gacggtcagc gtgaatttgg gtttgaaccg
     2041 gacggcggtg ttgtggtcgg ggctgattat tgtggctttg attacgtcgc tggttatcgt
     2101 tacggtcggc aatatcccgt ttatcgggct ggtcgtgccg aacatcatca gccgcctgat
     2161 gggcgacaag ctgcgacaaa gcctgcctgc ggtggccttg ctgggcgcat ctttggtgtt
     2221 gctgtgcgat attatcggac gcgtgattgt gtttccgttt gaaattccgg tctctacggt
     2281 ttttggtgta ttgggtacgg ctttgttttt gtggcttttg ttgaggaaac ccgcctatgc
     2341 cgtctgaaaa aaatatcggt tttatggcag gaagcagccg cccgttgtgg gtcgcctttg
     2401 cgctgttgct ggtttcctgc gtcctgttta tgacgctcaa cgtcaaaggc gattgggatt
     2461 ttgttttgca actgcggctg accaaacttg ccgcgctgct gatggtcgcc tatgcggtcg
     2521 gcgtgtccac gcaactcttc caaacgctga ccaataatcc gattctgacc ccttcaattt
     2581 tgggtttcga ttcgctgtat gtgtttttgc agaccttgct ggtgtttacg ttcggcggcg
     2641 tgggctatgc ttccctgccg ttgacgggca aattcggctt tgaactggtc gtcatgatgg
     2701 gcggctcgct gctgctgttc tacacgctca tcaaacaggg cggacgcgat ttgtcgcgca
     2761 tgattttaat cggcgtgatt ttcgggattt tgttccgcag cctgtcgtcg ctgctttcgc
     2821 gcatgatcga tcccgaagaa tttaccgccg cgcaggcgaa tatgtttgcc ggattcaata
     2881 ccgtccacag cgagcttttg ggcataggcg cgctgattct gctcgtcagc gcggcggtcg
     2941 tttggcgcga acgctaccgc ttggacgttt accttttggg gcgtgaccaa gccgtcaatt
     3001 tgggcatcag ctacacgcgc aacaccttat ggatactgct ttggattgcc gcattggtgg
     3061 cgacggcgac cgccgtggtc ggccccgtaa gctttttcgg gcttctcgcc gcctcgcttg
     3121 ccaaccactt ttccccgtcg gtcaaacatt ccgtccgcct gccgatgacg gtttgtatcg
     3181 gcggcatcct cttggtcggc ggacagaccg tgttcgaaca cctgctcggt atgcaggcag
     3241 tgttgagcgt agtagtagaa tttgccggcg gactcgtttt cctctatctc gttttaaaac
     3301 acaaaaaatg acggatgccg tctgaacggc cgccgccccg aaaggacaaa ccatatgaca
     3361 caagaacatt tcccatcatt cttcaaccaa gccccgacca ttaccgtcca agacgcattg
     3421 gccgaattcc tcggcgcggc cgaaaacggc atcctcactt accgctacgc cgatgccgtg
     3481 cgcctgtgcg gacattcctg cccgaccgtc gcgggcgcgt acctgatggt tatcaaaggt
     3541 ctgaaagcac tttacggcga agagctgccc gaacgcggcg gcatcgaagc ctttatgcag
     3601 ggcgcgcgcg acgaaggcac ggtcggcgta accgcgtccg tcgtccaact cctcaccggc
     3661 gcagcccccg aaaccggctt tggcggcatc ggaatgcagg gacgctttgc ccgccgccac
     3721 ctcttatcct ttggtgtagg cgaaatcaac ggcacactga ccctgcgccg caaagacaac
     3781 ggcaaaaccg tcgccgtcgg cctcaacgcc gccctgcaac ccttcgcacc cgaaatgcgc
     3841 gacatcatgc ccaaagccgt cagcggcagc gcaagcgcag aagaactcga acgcttcgga
     3901 caactctggc aggcacgcgt taaagcattt ttaaccgaat cggcggacga cccgcagttc
     3961 gtcatcgtcc gcgaagtgtg agcgttcaga cggcattccg aatttcaaat gccgtctgaa
     4021 ccccgccaaa caacaacaaa cctacgcccg acaagcatcc gccatgatta ccatccgcaa
     4081 cgtcagctac cgcatcggca cacgccccat cctcgacaac gtcagcctcg acatccccga
     4141 aggcggcatt accgccctcg tcggccccaa cggtgcgggc aaatccaccc tgttttcctt
     4201 tatggcgcgg ctgcgaccgc ttgaaagcgg cagcatcgcc taccgaggca aaaatcttgc
     4261 cgataccccc accgccgaac tcgccaaaac cctgtccatc ctcacccaag aaaacagcat
     4321 catgagccgc atcaccgtgc gcgacctgct gatgttcggc cgttacccct accatcaagg
     4381 cagaccgact gccgaatgcc gccgtatcgt taacggtgca atcgaagaat tccacctgca
     4441 agacctctcc gaccgctacc tgaccgagct ttccggcggc caacgccaac gcgccatgat
     4501 tgcgatggtg ttctgccaaa gcaccgacta cgtccttttg gacgaaccgc tgaacaacct
     4561 cgatatgtat cacgcccgct cgctcatgca aatcctgcgc cggctgaccg acgaacacaa
     4621 gcgcaccacc gtcgtcgtat tgcacgacat caaccaggca gcagcctacg ccgaccacgt
     4681 cgtcgccatg aaaaacggcc aagtcgccat gcagggcaaa cccaacgata ttttcaccgc
     4741 cgcaaacatc aaaaccctat tcgatatgga cgtcgacgtc ctcgattacg aaggcaaaaa
     4801 attggttatc caccatatct aaatccgaca aaaaggccgt ctgaacattc agacggcaac
     4861 ccatatcctg acaaaattaa gacacgacac cggcagaatt gacatcagca taatatgcac
     4921 atattaacag atattaatgc cgaactacct aactgcaaga attaaataaa taaataaata
     4981 aataaattgc gacaatgtat tgtatatatg cctcctttca tatatacttt aatatgtaaa
     5041 caaacttggt ggggataaaa tacttacaaa agatttccgc cccatttttt atccactcac
     5101 aaaggtaatg agcatgaaac actttccatc caaagtactg accacagcca tccttgccac
     5161 tttctgtagc ggcgcactgg cagccacaaa cgacgacgat gttaaaaaag ctgccactgt
     5221 ggccattgct gctgcctaca acaatggcca agaaatcaac ggtttcaaag ctggagagac
     5281 catctacgac attgatgaag acggcacaat taccaaaaaa gacgcaactg cagccgatgt
     5341 tgaagccgac gactttaaag gtctgggtct gaaaaaagtc gtgactaacc tgaccaaaac
     5401 cgtcaatgaa aacaaacaaa acgtcgatgc caaagtaaaa gctgcagaat ctgaaataga
     5461 aaagttaaca accaagttag cagacactga tgccgcttta gcagatactg atgccgctct
     5521 ggatgcaacc accaacgcct tgaataaatt gggagaaaat ataacgacat ttactgaag
//
LOCUS       DAWFAN010000131         5499 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWFAN010000131 DAWFAN010000000
VERSION     DAWFAN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.131"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            299..2428
                     /locus_tag="WGO65_001623"
     CDS             299..2428
                     /locus_tag="WGO65_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0327900.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPKAQKDHGGYGFAMRLKRRNWYPKAEESEVKLNESDWEATGLPTDPKELPK
                     RQKSVIEQVKTDDNSNIYSSPYLTPSNHQNGNATNQPKNEVKDYKEFKYVYSGWFYKH
                     AKQDIDSTNKIVRQGDDGYIFYHGKEPSRQLPASEKVIYKGVWHFVTDTKKGQEFREI
                     IQPSKKQGDRYSGFSGDDNEEYSNKKETILQNGHEGYGFTSNLEVDFGNKKLTGKLIR
                     NNASLNNNDKHTTQYYSLDATLKGNRFSGTATATATDKKENETKQHPFVSDSSSLSGG
                     FFGPQGEELGFRFLSDDQKVAVVGSAKTKDKAANGNTVAASGGTDAAASNGAAGTSSE
                     NSKLTTVLDAVELKSGGKEVQKLDNFSNAAQLVVDGIMIPLLPETSESGNNQANQGKN
                     GGTAFTYATTYTPESDKKDTQAQTVTGGTQTASNTAGDANGKTKTYAVEVCCSNLNYL
                     KYGLLTRKTVGNTREGGNSSPTAAAQTAQGAQSMFLQGERTDEKEIPKEQNVVYRGSW
                     YGYIANDTSWSGNASDKEGGNRAEFTVNFDTKKINGTLTADNRQAATFTIEGDIKDNG
                     FEGTAKTADSGFDLDQSNNTRTPKAYITDAKVQGGFYGPKAEELGGWFAYSGDKQTEN
                     TTVASGNGNSASSATVVFGAKRQQPVR"
     gene            2515..5259
                     /gene="tbpA"
                     /locus_tag="WGO65_001624"
     CDS             2515..5259
                     /gene="tbpA"
                     /locus_tag="WGO65_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ0327901.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTNQYAHFVVEEECPNGYAACKDKPK
                     KDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHTQ
                     QTFDTRDMTVPAFLTKAVFDANKKQAGSLSGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYFQNAKRAYSSNLPTQNGNQKISPNGSTSTPYWVSIGRGNVVTGQICRLGNN
                     TYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLS
                     WNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIV
                     FKGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINILGK
                     IDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQ
                     PEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIK
                     KHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF
                     "
BASE COUNT         1590 a         1359 c         1401 g         1149 t
ORIGIN      
        1 tatcgggtaa aacggtttct tgagattttg cgtcctggat tcccactttc gtgggaatga
       61 cgggatatat gtgggaatga cgggatatac gtgggaatga cgggatatac gtgggaatga
      121 cgggatatac gtgggaatga cgggatatag gtttccgtgc ggacgcgttc ggattcacga
      181 ctgcgcggga atgacgggat tttggtgtat tccctaaaaa ataaaaaagt atttgcaaat
      241 ttgttaaaaa taaataaaat aataatcctt atcattcttt aattgaattg gatttattat
      301 gaacaatcca ttggtgaatc aggctgctat ggtgctgcct gtgtttttgt tgagtgcttg
      361 tttgggcgga ggcggcagct tcgatcttga ttctgtcgat accgaagccc cgcgtcccgc
      421 gccaaaatat caagatgttt cttccgaaaa gccgaaagcc caaaaagacc acggcggata
      481 cggttttgcg atgaggttga aacggaggaa ttggtatccg aaggcagaag aaagcgaggt
      541 taaactgaac gagagtgatt gggaggcgac aggattgccg acagacccca aggaactccc
      601 taaacggcaa aaatcggtta ttgaacaagt aaaaacagac gacaacagca atatttattc
      661 ttccccctat ctcacaccat caaaccatca aaacggcaac gctacaaacc aaccaaaaaa
      721 tgaagtaaaa gattataaag agttcaaata tgtttattcc ggttggtttt acaaacacgc
      781 taaacaagat attgattcga caaataaaat tgtacgacaa ggcgacgacg gttatatctt
      841 ttatcacggc aaagaacctt cccgacaact tcctgcttct gaaaaagtta tctacaaagg
      901 tgtgtggcat tttgtaaccg atacaaaaaa gggtcaagag tttcgtgaaa ttatccagcc
      961 ttcaaaaaaa caaggcgaca ggtatagcgg attttcgggt gatgacaacg aagaatattc
     1021 caacaaaaag gaaacgattt tacaaaacgg tcatgaaggt tatggtttta cctcgaattt
     1081 agaagtggat ttcggcaata agaaattgac gggtaaatta atacgcaata atgcgagcct
     1141 aaataataat gacaaacata ccacccaata ctacagcctt gatgcgacgc ttaagggaaa
     1201 ccgtttcagc ggcacggcaa cggcaacggc aactgacaaa aaagagaatg aaaccaaaca
     1261 acatcccttt gtttccgact cgtcttcttt gagcggcggc tttttcggcc cacagggtga
     1321 ggaattgggt ttccgctttt tgagcgacga tcaaaaagtt gccgttgtcg gcagcgcgaa
     1381 aaccaaagac aaagccgcaa atggcaatac tgtggcggct tcaggcggca cagatgcggc
     1441 agcatcaaac ggtgcggcag gcacgtcgtc tgaaaacagt aagctgacca cggttttgga
     1501 tgcggtcgag ctgaaatcgg gcggtaagga agtccaaaag ctcgacaact tcagcaatgc
     1561 cgcccaactg gttgtggacg gcattatgat tccgctcctg cccgagactt ccgaaagtgg
     1621 gaacaatcaa gccaatcaag gtaaaaacgg cggaacagcc tttacctacg caacaaccta
     1681 cacgccggaa agtgataaaa aagacaccca agcccaaaca gtcacgggcg gcacgcaaac
     1741 cgcttcaaat acggcaggtg atgccaatgg caaaacaaaa acctatgcag tcgaagtctg
     1801 ctgttccaac ctcaattatc tgaaatacgg gttgctgacg cgcaaaactg tcggcaatac
     1861 gagggaaggc ggcaacagca gcccaaccgc cgccgcccaa acggcacagg gtgcacaaag
     1921 tatgttcctc caaggcgagc gtaccgatga aaaagagatt ccaaaagagc aaaacgtcgt
     1981 ttatcggggg tcttggtacg ggtatattgc caacgacaca agctggagcg gcaatgcttc
     2041 agataaagag ggcggcaaca gggcggaatt tactgtgaat ttcgatacga aaaaaattaa
     2101 cggcacgtta accgctgaca acaggcaggc ggcaaccttt actattgagg gcgatattaa
     2161 ggacaacggc tttgaaggta cggcgaaaac tgctgactca ggttttgatc tcgatcaaag
     2221 caataacacc cgcacgccta aggcatatat cacagatgcc aaggtgcagg gcggttttta
     2281 cgggcccaaa gccgaagagt tgggcggatg gtttgcctat tcgggcgata aacaaacgga
     2341 aaatacaaca gttgcatccg gcaatggaaa ttcagcaagc agtgcaactg tcgtattcgg
     2401 tgcgaaacgc caacagcctg tgcgataagc acggttgccg aacaatcaag aataaggctt
     2461 cagacggcat cgctccttcc gatgccgtct gaaagcgaag attagggaaa cattatgcaa
     2521 cagcaacatt tgttccgatt aaatatttta tgcctgtcgc tgatgaccgc gctgcccgtt
     2581 tatgcagaaa atgtgcaagc cggacaagca caggaaaaac agttggatac catacaggta
     2641 aaagccaaaa aacagaaaac ccgccgcgat aacgaagtaa ccggtttggg caaattggtc
     2701 aaaaccgccg acaccctcag caaggaacag gtactcgata tccgcgacct gacgcgttac
     2761 gaccccggca tcgccgtcgt cgaacagggg cgcggcgcaa gctcgggtta ctcgatacgc
     2821 ggtatggaca aaaaccgcgt ttccttgacg gtggacggct tggcgcaaat acagtcctac
     2881 accgcgcagg cggcattggg cgggacgagg acggcgggca gcagcggcgc aatcaatgaa
     2941 atcgagtatg aaaacgtcaa ggctgtcgaa atcagcaaag gctcaaactc ggtcgaacaa
     3001 ggcagcggcg cattggcggg ttcggtcgca tttcaaacca aaaccgccga cgatgttatc
     3061 ggggaaggca ggcagtgggg cattcagagt aaaaccgcct attccggcaa aaaccggggg
     3121 cttacccaat ccatcgcgct ggcggggcgc atcggcggtg cggaggcttt gctgatccac
     3181 acgggtcggc gcgcggggga aatccgcgcc cacgaagatg caggacgcgg cgttcagagc
     3241 tttaacaggc tggtgccggt tgaggatacc aatcagtacg ctcatttcgt ggtcgaagaa
     3301 gaatgcccta atggatatgc ggcttgtaaa gacaaaccga aaaaagatgt tgtcggcaaa
     3361 gacgaacgtc aaacggtttc cacccgagac tacacgggcc caaaccgctt cctcgccgat
     3421 ccgctttcat acgaaagccg atcgtggctg ttccgcccgg gttttcgttt tgaaaacaaa
     3481 cggcactaca tcggcggcat actcgaacac acgcaacaaa ctttcgacac gcgcgatatg
     3541 acggttccgg cattcctgac caaggcggtt tttgatgcaa ataaaaaaca ggcgggttct
     3601 ttgtccggca acggcaaata cgcgggcaac cacaaatacg gcggactgtt taccaacggc
     3661 gaaaacggtg cgctggtggg cgcggaatac ggtacgggcg tgttttacga cgagacgcac
     3721 accaaaagcc gctacggttt ggaatatgtc tataccaatg ccgataaaga cacttgggcg
     3781 gattatgccc gcctctctta cgaccggcag ggcatcggtt tggacaatca ttttcagcag
     3841 acgcactgtt ctgccgacgg ttcggacaaa tattgccgcc cgagtgccga caagccgttt
     3901 tcctattaca aatccgaccg cgtgatttac ggggaaagcc acaggctctt gcaggcggca
     3961 ttcaaaaaat ccttcgatac cgccaaaatc cgccacaacc tgagcgttaa cctcggtttt
     4021 gaccgctttg gctctaatct ccgccatcag gattattatt ttcaaaacgc caaacgtgcc
     4081 tattcgtcga atctgcccac tcaaaacggc aatcaaaaaa tcagccccaa cggcagcaca
     4141 agcactccct attgggtcag cataggcagg ggaaatgtgg ttacggggca aatctgccgc
     4201 ttgggcaaca atacttatac ggactgcacg ccgcgcagca tcaacggcaa aagctattac
     4261 gcggcagttc gggacaatgt ccgtttgggc aggtgggcgg atgtcggcgc ggggttgcgc
     4321 tacgactacc gcagcacgca ttcggacgac ggcagcgttt ccaccggcac gcaccgcacc
     4381 ctgtcctgga acgcaggcat cgtcctcaaa cctgccgact ggctggattt gacttaccgc
     4441 acttcaaccg gcttccgcct gccctcgttt gcggaaatgt acggctggcg ggcgggtgtt
     4501 caaagcaagg cggtcaaaat cgatccggaa aaatcgttca acaaagaagc cggcatcgtg
     4561 tttaaaggtg atttcggcaa cttggaggca agttggttca acaatgccta ccgcgatttg
     4621 attgtccggg gttatgaagc gcaaattcaa aacggcaaag aaacagccaa aggcgacccg
     4681 gcttacctca atgcccaaag cgcgcggatt accggcatca atattttggg caaaatcgat
     4741 tggaacggcg tatgggataa attgcccgaa ggttggtatt ctacatttgc ctataatcgt
     4801 gtccgtgtcc gcgacatcaa aaaacgcgca gaccgcaccg atattcaatc acatctgttt
     4861 gatgccatcc aaccttcgcg ctatgtcgtc ggcttgggct atgaccaacc ggaaggcaaa
     4921 tggggtgtga acggtatgct gacttattcc aaagccaagg aaatcacaga gttgttgggc
     4981 agccgggctt tgctcaacgg caacagccgc aatacaaaag ccaccgcgcg ccgtacccgc
     5041 ccttggtata ttgtggatgt gtccggttat tacacgatta aaaaacactt caccctccgt
     5101 gcgggcgtgt acaacctcct caactaccgc tatgttactt gggaaaatgt gcggcaaact
     5161 gccggcggcg cagtcaatca acacaaaaat gtcggcgttt acaaccgata tgccgccccc
     5221 ggccgaaact acacatttag cttggaaatg aagttttaaa cgtccaaacg ccgcaaatgc
     5281 cgtctgaaag gcttcagacg gcatttttta cacaattccc accgttttcc atcatccccg
     5341 acaataccgt aatcttgaaa ttcgtcattc ccgcgcaggc aggaatctag accgttcggt
     5401 ttaggttttt ttgaggtttc gggtaacttc taaaccgtca ttcccgcgaa aacagaaaat
     5461 caaaaacaga aacctcaaat cccgtcattc ccgcgaaag
//
LOCUS       DAWFAN010000132         5463 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWFAN010000132 DAWFAN010000000
VERSION     DAWFAN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.132"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            244..5409
                     /gene="iga"
                     /locus_tag="WGO65_001625"
     CDS             244..5409
                     /gene="iga"
                     /locus_tag="WGO65_001625"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050177927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ0327902.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLANNERDANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVTDGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTSHASTITLTGKSLITAPQNLSVYEIRNDYDDDDYYGYYSYRKP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGVTENNDWVFMGYTQEEAKKNAMNHKNN
                     QRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGANLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEITVNQSASFSSGRNVS
                     DITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKALNSFGATQINGNV
                     NLSQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVRDRSHLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PVPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQTEEAKRQQAEAERKSAE
                     LAKQKAEAEREARELATRQKAEQERSTAELARRHEKEREAAELSAKQRAGEEERRQTA
                     QSQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAE
                     LLAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSAKQKAAAEAQ
                     ALAARQQKALARQQEEARKAAELAAKQKAETERKTAELAKQRAAAEAAKRQQEVRQAA
                     ELARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQ
                     DYMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDAFED
                     EDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRSQAAAQPQAQAAAQADAISTN
                     TNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRR
                     FSSKRTQTQIGIDRSLSENMQMGGVLTYSDSQHTFDQANGKNTFVQANLYGKYYLNDA
                     WYVAGDIGAGSLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLS
                     SADYKLGDDSVKVSSMAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVG
                     GNSFAYKADNQQQYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1824 a         1317 c         1212 g         1110 t
ORIGIN      
        1 cacaagcggg aatccaaaaa caaaaagccg cagaaattta acggaagaac cgaaccccga
       61 aaaccggctt cccgccttcg cttgaataac agaacaaagg aagcggaaat aaaaacatcg
      121 ggattctttt tttaaattgg caataaaaca ccaaaattaa tgatttacac aacaaaaatt
      181 agtcaacacc gccaaaccgg cgtaaaatac aaaacattat cacccttaaa acggtaaaac
      241 cttatgaaaa ccaaacgttt taaaattaac gccatatcct tatccatctt tcttgcctat
      301 gcccttacgc catactcaga agcggcattg gtcagagacg atgtcgatta tcaaatattc
      361 cgtgactttg cagaaaacaa aggcaaattt tttgtcggcg caaccgattt atcagtgaaa
      421 aacaaacaag gtcaaaacat cggcaacgca ttatccaacg taccgatgat tgattttagc
      481 gttgcagatg tcaacagacg cacattaacc gtcatcgatc cccagtatgc cgtcagcgtc
      541 aaacatgtaa aaggagacga aatctcttat tacgggcatc acaatgggca cttagatgtt
      601 agcaatgatg aaaacgaata tcgttcagtt gcacaaaatg actacgaacc aaataaaaat
      661 tggcatcacg gtaatcaagg tcgtcttgaa gactacaaca tggcacgcct taataaattc
      721 gtaacagaag tcgcacctat tgcaccaacc agtgcaggcg gcggcgttga aacctataaa
      781 gataaaaacc gtttttctga gtttgtgcga gttggagcag gtacgcaatt tgaatataac
      841 agccgctaca atatgacaga gctttcacga gcttatcgtt atgccattgc aggtacgcct
      901 tatcaagacg tgaatgtcac atcgaatcta aatcaagaag gcttgattgg cttcggtgat
      961 aattcaaaac atcattcccc agaaaaactc aaagaagttt tatctcaaaa cgcattgact
     1021 aactatgctg tgttaggcga tagcggttca ccattatttg cttatgataa acaagaaaaa
     1081 cgctgggtct ttttaggtgc ttacgattat tgggcaggct atcaaaaaaa ctcttggcaa
     1141 gaatggaata tctataaaaa agaatttgca gatgaaatca aacaacgcga taacgccggc
     1201 accatcaaag gttatggcga acatcattgg aaaaccacgg gcacaaacag ccatatcggt
     1261 tcgacagcgg taaggcttgc caacaatgaa agggatgcaa acaacggaca gaatgttacc
     1321 tttgaaaaca acggcacttt ggtattggat caaaacatca accaaggcgc gggcggtctg
     1381 tttttcaaag gcgattacac agtcaaaggt ataaataatg acatcacttg gctaggtgcg
     1441 gggattgatg ttaccgacgg caaaaaagtc gtttggcaag tcaaaaatcc gaatggcgac
     1501 agattggcaa aaatcggcaa aggcacatta gaaataaacg gcacaggcgt taaccaaggg
     1561 caattaaaag tcggcgacgg tacggttatt ctgaatcaac aagccgatgc cgacaaaaaa
     1621 gtccaggctt tctcccaagt cggcattgtc agcggacgcg gtacattggt attaaatagt
     1681 tcaaatcaga ttaatcccga taacctatat ttcggtttcc gtggcggtcg tttggatgcc
     1741 aatggcaacg acttgacttt tgaacacatc cgcaacgtgg atgaaggcgc gcgcattgtc
     1801 aaccacaaca caagccacgc ctccacaatc acattaaccg gcaaatcatt aattaccgct
     1861 cctcaaaatc tttctgttta tgaaatccga aatgattacg atgatgacga ttattatggc
     1921 tattacagct atagaaaacc cattccgcaa ggcaaagatc tttattacaa aaactatcgt
     1981 tattacgccc taaaatccgg cggcagcgtg aacgcaccga tgcccgagaa cggtgtaaca
     2041 gaaaataacg actgggtctt tatgggatat acgcaagaag aagccaagaa aaacgcgatg
     2101 aaccacaaaa acaatcagcg tattagcggt tttagcggtt tctttggcga agaaaacgga
     2161 aaaggacata acggcgcatt aaaccttaat ttcaacggca aaagcgcgca aaaccgtttc
     2221 ttgttaacag gcggtgccaa tttaaacgga aaaataagcg taactcaagg caatgtcttg
     2281 ttatcaggtc gtccaacacc acacgcaaga gattttgtga acaaatcttc agcccgaaaa
     2341 gacgcacatt tttccaaaaa caatgaagtc gtatttgaag acgactggat aaaccgcaca
     2401 ttcaaagccg cagaaattac ggttaatcaa tccgcatcat tctcttccgg cagaaatgta
     2461 tccgacatca ccgccaacat taccgcaaca gacaacgcca aagtaaattt gggttacaaa
     2521 aacggcgatg aggtttgcgt gcgctcggac tataccggtt acgttacctg caacacagac
     2581 aacttatccg ataaggcttt aaacagcttt ggagcgacac agattaacgg caatgtgaat
     2641 ttgagtcaga atgcggcatt ggtcttaggc aaggctgcat tatgggggca aattcaaggg
     2701 caaggaaaca gccgtgtcag cctaaaccaa catagcaaat ggcatttgac cggcgacagc
     2761 caagtacaca atctgtcatt ggcggatagc catattcatt tgaacaacgc ttccgacgcg
     2821 caaagtgcaa ataaatacca cacgctcaaa atcaatcatt tatccggtaa cgggcatttt
     2881 cattatctga cgcacctggc gaaaaatctt ggggataaag tgcttgtgaa ggaatccgca
     2941 tccggtcatt atcagctcca tgttcaagat aaaacaggcg aacccaatca ggaagggctg
     3001 aatctctttg atgcatcatc cgtacgagac cgttcgcacc tttctgtttc cttggcgaat
     3061 aaccatgtag atttaggcgc attgcgttat acaatcaaaa cagaaaacgg tattacccga
     3121 ttgtacaatc cttatgccga gaaccgccgc cgagtcaaac cggtcccgtc tcctgccaca
     3181 aacacggctt ctcaggcaca aaaggcaaca caaacggacg gtgcacaaat tgccaagcct
     3241 caaaatatcg tcgtcgcacc gcctagcccg caggcaaatc aaaccgaaga agcaaaacgc
     3301 cagcaagcag aagcagagcg taaatcggca gagttagcaa aacaaaaagc agaagccgaa
     3361 cgcgaagcaa gagagctggc gacccgccaa aaagcagaac aagaacgcag caccgccgaa
     3421 cttgcacgcc ggcatgaaaa agaacgcgaa gcggcagagt tgtcggcaaa acaaagggcc
     3481 ggggaagagg aacgccgtca aacagcccaa tcccagccgc aacgccgcaa acgccgtgcc
     3541 gcaccgcagg attatatggc agcttcccaa gaccgtccta aacgccgcgg acaccgttcg
     3601 gtacagcaaa acaatgtgga aattgcccaa gcccaagcag aacttgcgcg ccgacagcag
     3661 gaagaacgca aagcggcaga gttgttggca aaacaaaggg cagaagccga acgtgaggct
     3721 caagcattgg ctgcgcgccg aaaagctgaa gccgaagagg caaaacgcca agctgccgaa
     3781 cttgcacatc gccaggaagc agaacgcaaa gcggcagagt tgtcggcaaa acaaaaagcc
     3841 gcagctgaag cccaagcatt ggcagcacgc caacaaaaag cccttgcgcg tcaacaggaa
     3901 gaagcacgaa aagctgcaga gttagccgcc aaacaaaaag cggaaaccga acgcaaaacc
     3961 gccgaacttg ccaagcaaag ggcagcagcc gaagcagcaa aacgccagca agaagttcgc
     4021 caggccgccg aacttgcgcg tcgtcaagaa gcggaacgcc aagcagcaga gttgtcggca
     4081 aaacaaaaag ccgaaacaga tcgagaagcg gcagaatccg cgaaacgaaa agctgaggaa
     4141 gaagagcacc gtcaagcagc ccaatcccag ccgcaacgcc gaaaacgccg tgccgcacct
     4201 caggattata tggcagcttc ccaaaatcgt ccgaaacgtc gcggacgcag atctactctg
     4261 ccggcaccgc cctcgccatc atttgattca tcagcttacg cagcacccag ggccttgcat
     4321 aatccggact ggtatgaaaa tgactatgaa gaaatcccct tggatgcgtt tgaagatgaa
     4381 gatgtatccg aatcggttga cacatcagac aaacagcctc aagacaatac ggaacttcat
     4441 gaaaaagttg agacagtgag tttgcaacca agagccgcgc agccgcgatc ccaagccgcc
     4501 gctcagccgc aagcccaagc cgccgcgcaa gccgatgcaa tcagcaccaa taccaactcg
     4561 gctttatctg acgcaatggc aagcacgcaa tctatcttgt tggatacagg tgcttcatta
     4621 acacggcaca ttgcacaaaa atcacgcgct gatgcagaaa aaaacagtgt ttggatgtcg
     4681 aacaccggtt atggccgtga ttatgcttcc gcacaatatc gccggtttag ttcgaaacgc
     4741 acgcaaacac aaatcggcat tgaccgcagc ttgtccgaaa atatgcagat gggcggagta
     4801 ttgacttact ctgacagtca gcatactttt gatcaggcga acggcaaaaa tacttttgtg
     4861 caagccaacc tttatggtaa gtattattta aatgatgctt ggtatgtggc cggcgatatt
     4921 ggtgcgggca gcttgagaag ccggttacaa acgcagcaaa aagcaaactt taaccgaaca
     4981 agcatccaaa ccggccttac tttgggcaat acgctgaaaa tcaatcaatt cgagattgtc
     5041 cctagtgcgg gtatccgtta cagccgcctg tcatctgcag attacaagtt gggtgacgac
     5101 agtgttaaag taagttctat ggcagtgaaa acactaacgg ccggactgga ttttgcttat
     5161 cggtttaaag tcggcaacct taccgtaaaa cccttgttat ctgcagctta ctttgccaat
     5221 tatggcaaag gcggcgtgaa tgtgggcggt aattccttcg cctataaagc agataatcaa
     5281 cagcaatatt cagcaggtgc cgcgttactg taccgtaatg ttacattaaa cgtaaatggc
     5341 agtattacaa aaggaaaaca attggaaaaa caaaaatccg gacaaattaa aatacagatt
     5401 cgtttctaaa atactaaatt catagcaaaa taaaatgccg tccgaactca ggcttcagac
     5461 ggc
//
LOCUS       DAWFAN010000133         5420 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWFAN010000133 DAWFAN010000000
VERSION     DAWFAN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.133"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..297)
                     /locus_tag="WGO65_001626"
     CDS             complement(<1..297)
                     /locus_tag="WGO65_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0327903.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRH"
     gene            536..1435
                     /gene="crgA"
                     /locus_tag="WGO65_001627"
     CDS             536..1435
                     /gene="crgA"
                     /locus_tag="WGO65_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ0327904.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSAEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1662..4877)
                     /gene="carB"
                     /locus_tag="WGO65_001628"
     CDS             complement(1662..4877)
                     /gene="carB"
                     /locus_tag="WGO65_001628"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ0327905.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLVKEEKAVSDGILSDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICTTRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRS"
     gene            complement(4906..>5420)
                     /locus_tag="WGO65_001629"
     CDS             complement(4906..>5420)
                     /locus_tag="WGO65_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327906.1"
                     /translation="KPDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFI
                     NFFKINVQAGAAKIREIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIY
                     CYDVRRNGKDKAGKPNRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDA
                     EIIREVTNRQ"
BASE COUNT         1221 a         1385 c         1409 g         1405 t
ORIGIN      
        1 gtgtctgccg tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta
       61 ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa tcacagcatc
      121 catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc
      181 ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt
      241 taacccgccg tgagaatgtc cgaacgcttt gccgccgtct aataataaaa tattcatttg
      301 ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc
      361 aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgttccaac acacgggatg
      421 gcacataaag caccgccctg tgtgccgtcc tgattcggaa ggggttacac ccctcccaaa
      481 taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa acacgatgaa
      541 aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca gcttcagccg
      601 tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca aacggctgga
      661 ggaaaagttg ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc tgacggaaga
      721 aggcgcacaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag cggcggaaac
      781 cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt ccgcgatgcc
      841 gatggtgctg catctgctgg cgccgctggc agcaaaattc aacgaacgct atccgcatat
      901 ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca aagtcgatat
      961 tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc tgtttgacag
     1021 ccgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc cgcaatctgc
     1081 agaagagctt gccggtcacc aatgtttagg cttcaccgaa cccggttctc taaatacatg
     1141 ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta ccgccagcag
     1201 cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct tatcagattt
     1261 tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg aacaaacctc
     1321 caataaaacg caccccttta atgctgttta ttacagcgat aaagccgtca acctccgctt
     1381 gcgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg gataattcta
     1441 taaaaacctg ccataaaatt gtttttactc acttaataac tttgagaaaa aataaataag
     1501 atgatgtata gtcggcggca atggcagtct atatggggag cgtgataggg ctgttcctta
     1561 tgtacggctt gtgtgtggca acatcggctt tccaaatgac aaaatgccgt ctgacaccgt
     1621 aaaaaaaggt ttcagacggc attttcaacc cgattcaggc atcagctgcg gtttttcaaa
     1681 cgcccgtgca gttcttgaac gctgtacacg cccaagtggt cgcggctttt cgcgccttcg
     1741 ctcatcgctt cgccgcccgc agtagtggtg tattgcggca cacgttgctg caatgcgctt
     1801 tggcggatga tgtggctgtc ggacacggat tgcggatcgc tggaaacggt attcacgacc
     1861 agtgcgattt cgccgttttt cagcgcgtcg ccgatgtgcg ggcggccttc ggggacttta
     1921 ttgatggttt gtacaatcag cccgtgttcg gtcaggtatt gcgccgtgcc gcgcgtggtg
     1981 cagatgccgt agcctaaggc ttggaagttt ttggcggttt taatgacgcg ttctttgtct
     2041 tcttcgcgca cggagaggaa gattttgccg gtcgggttga ggcgttcgcc cgcgccgagt
     2101 tgggctttgt agtaggcttc gccaaagctt gcgcccacgc ccatgacttc gccggtggag
     2161 cgcatttccg gtcccaaaat cgtatccacg cccgggaatt tgatgaatgg gaacacggct
     2221 tctttaacgg catagaaatc ggggacaact tctttttcca cgccttgttc tttcagggaa
     2281 atgcctgcca tacagcgtgc gccgactttg gcgagcggca cgccggtggc tttggagacg
     2341 aagggcacgg tacggctggc gcgcgggttc acttccaaca cgaacaccac gccgtcttgt
     2401 acggcaaact gcacgttcat cagtccgacc acgcccagcg cgtacgccat ggctttggtt
     2461 tggcggcgga tttcgtcttg gatttcttcg ctgagcgagt agggcggcag cgagcagccg
     2521 gagtcgccgg agtggatgcc cgcctgttcg acgtgctgca tgatgccgcc gataaccaca
     2581 tctttgccgt ctgaaacgca gtccacatcg acttcaatcg cgttgttgag gaagaaatcg
     2641 agcaacacgg ggctgtcttc ggaaacttgt acggcttcgc gcatatattt ttgcaactgc
     2701 tcggcagagt gaacaatctg catcgcgcgt ccgcccaaaa cataagacgg gcgcacgacc
     2761 agcggatagc cgatttcttc ggctttgacg agtgcttctt cttcgttgtg ggcgatgcgg
     2821 ttgggcggtt ggcgcagacc taagtcgttc aacacttttt ggaagcgttc gcggtcttcg
     2881 gcggcgtcga tgctgtcggc ggacgtgccg atgatgttta cgccgttttc aaccaacgcg
     2941 ttggcgagtt tcagcggggt ttgaccgccg taatgcacaa tcacgcccca cgggttttcg
     3001 gtgcggacga tttccaacac gtcttccaat gtcagcggct cgaaatagag gcggtcgctg
     3061 gtgtcgaagt cggtggacac ggtttcgggg ttgcagttga ccatgatggt ttcaaagccc
     3121 gattcgcgca gggcgagtgc ggcgtgaacg cagcagtagt caaactcgat gccctgaccg
     3181 atgcggttcg ggccgccgcc gagaatcatc acttttttac ggtcggaagg acgggattcg
     3241 cattcttctt cgtaagtgga gtaaagatag gcggtttcgg tggcgaactc ggcggcgcag
     3301 gtatcgacgc gtttgtaaac cggatgcagc ttcagcgcgt agcggtgttc gcgaacttct
     3361 ttttcgctta cgttcaacaa ttgtgccaaa cgtttgtcgg agaagccttt gcgtttcaga
     3421 cggcgtaggg cggcataatc caaatcactc aaaatgccgt ctgaaaccgc tttttcttct
     3481 ttcaccaagt cttcgatttg cgccaagaac caagggtcga tggcgcagat ttcgtggatt
     3541 tcttccaaag taaagcctgc gcggaacgca tcggctacaa agagcatgcg ctcaggaccc
     3601 gggttggcca gttcgcggcg gatttccgct ttgtcttcgc tgcgagggtt gaagccgcac
     3661 aagccggttt ccaaaccacg cagggctttt tggaagcttt cctgaatggt acggcccatt
     3721 gccattactt cgcccacaga tttcatttgc gtggtcaggc ggtcgtctgc ggcagggaat
     3781 ttttcaaacg cgaaacgcgg gattttggtt accacatagt cgatggaagg ctcgaacgac
     3841 gcgggcgtgc ggccgccggt gatgtcgttg cgcaactcgt ccagcgtaaa gccgaccgcc
     3901 agcttcgccg ccaccttcgc aatcgggaaa cccgtcgcct tggaagccag cgcggacgag
     3961 cggctcacgc gcgggttcat ctcaatcaca atcatctcgc cgttttcagg gttcaccgca
     4021 aactgaacgt tcgagccgcc ggtatccaca ccgatttcgc gcaataccgc caagcttgcg
     4081 ttgcgcatga tttggtattc tttgtccgtc agcgtttgcg ccggcgcaac agtgatggag
     4141 tcgcccgtat gcacgcccat cgggtcgaag ttttcaattg agcaaataat gatgcagttg
     4201 tcggccttat cgcgcaccac ttccatctcg tactctttcc agccgagcac ggactgctca
     4261 atcagcagct catgcgtggg cgacgcatcg aaaccgcgtt cgcaaatcgc caaaaactcg
     4321 tctttattgt aggcaatgcc gccgcccgaa ccgcccatgg tgaaagaagg acgaatcagg
     4381 gtagggaagc cgacctgttc ttgcgccgcc aaagcttcgt tcatcgtgtg gcagacaaaa
     4441 gatttcgggc aggagaggcc gattttctcc atcgcctcct taaagcggcc gcggtcttct
     4501 gctttgtcga tggcgtcttc ggtcgcgccg atcagctcga cattgtattt cgccagcacg
     4561 ccgttgcgcg ccaaatccag cgcacagttc agcgcggtct gaccacccat cgtgggcaga
     4621 atcgcatcag gccgctcttt ggcgataatc ttctccaccg tctgccacat aatcggctcg
     4681 atgtaggtaa catccgccat ttcagggtcg gtcataatcg tggcggggtt ggaattcacc
     4741 agaatgactt tatagccttc ttcacgcaaa gccttgcagg cctgtgcgcc cgaatagtca
     4801 aattcgcagg cctgaccgat aacgataggg ccggcgccga tgataaggat ggattttagg
     4861 tcggtacgtt tgggcatggt gggtctttct tttgaagaat ttggattatt gacgattggt
     4921 aacttctcta attatttctg catctgtttt ttcatcgctt ggattatctg aaaagaaaaa
     4981 actgtaatta ctatcggctt taaatttttc gtatagctct gggcgcaaaa tttttgcttt
     5041 ttcactattt tccacgctcc tgcgattagg cttgccagcc ttatctttcc catttctacg
     5101 gacatcatag caataaatag tgttaggaga attaggttct aaacgagtag ctacaatctt
     5161 tttataaatt atgtctaaat cttttttagt ttgtgttgga atgaaatctg caaaaagatt
     5221 actaaaactc tgaaaaattt ctctgatttt cgcagcacca gcctgaacat taattttaaa
     5281 aaaattaata aaatcagtag cacttttaaa atgaacttca taaaaatcag catacgcaaa
     5341 tattgaattt tcactattca aattaaaaaa ctctaatttt gcttgtgcat aatcatctgg
     5401 tctaggttca tcaggtttat
//
LOCUS       DAWFAN010000134         5361 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWFAN010000134 DAWFAN010000000
VERSION     DAWFAN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.134"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(244..2520)
                     /locus_tag="WGO65_001630"
     CDS             complement(244..2520)
                     /locus_tag="WGO65_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0327907.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHGTEQSVGLETISVVGKSRP
                     HATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINVGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHAHNGKPWIDLRNKR
                     YELRAEWKQPLPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENHYNYPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDNQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            2931..3521
                     /locus_tag="WGO65_001631"
     CDS             2931..3521
                     /locus_tag="WGO65_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ0327908.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVVVRRNDDITLEEIEALKP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVIRAKTLMHG
                     KVSPVSHSGKGMFKDLPNPVTCTRYHSLVIARDTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3585..4643
                     /gene="trpD"
                     /locus_tag="WGO65_001632"
     CDS             3585..4643
                     /gene="trpD"
                     /locus_tag="WGO65_001632"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ0327909.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKMPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4795..5010)
                     /locus_tag="WGO65_001633"
                     /pseudo
     CDS             complement(4795..5010)
                     /locus_tag="WGO65_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5112..5361
                     /locus_tag="WGO65_001634"
                     /pseudo
     CDS             5112..5361
                     /locus_tag="WGO65_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
BASE COUNT         1281 a         1213 c         1477 g         1390 t
ORIGIN      
        1 ctgaaaccga atgaattgga ttcccgcctg cgtgggaatg atgggatctt gggtttttgc
       61 ttttgatttt tctgcttttg cgagaatgac ggcgtgaaag caagaatgat gaaacaaaaa
      121 aaatgggaat gatggcatag tggtttgttc tttgtctttg ccatatttcc taacaaattg
      181 attaaaaaga aaaaaggttt tcagaatgcc gtctgaaaac cttttttgtt tgcctgtccg
      241 attttaaaac ttcacgttca cgccgccggt aaagctgcgg cccatttgcg gcgtatcaga
      301 gagaaagctg ctgtgggcgt aaacggattg gttgagcagg ttgtcggctt tgacgtacca
      361 attccactcg ccatagcgcg tattgcggcg gtagtttgcg ccgaggttga gcatatggtg
      421 tccgggcgtg cgcgtttcgt agcgggcgag tttgttttgg gcgaacacgc ggtagtagtc
      481 caaattggca tcgatgcggt cggtcagcga ggctttcagg tgaacgccga ggcgcgcagc
      541 cggaacgcga ggggcgtttt ggttatcctg tgcaatgaaa ggacggttgc cgtaggcatc
      601 ttccctgcca ggtagggaag gcaggttttt cagacggcct cgtacatagt cgccggaaac
      661 gccgatgcgg tagcgcggtg tcggtttgaa gtagatttcg ccttccgcgc cgtagaagtc
      721 cgcaccggat tggttgtagc gcacgagctt catttcgctg tcgtcttcga tggatttggg
      781 gccgcgtccg tcgtttaagg tttgggcgta gatgtagttg ccgaaacggt tgcggtagag
      841 tgccagattg tattgccagc ggtcgccttc gtagcccagt gcgagttcga tattgttgga
      901 acgttctttg ttgaggtgtt tgttgccgac ttcaaaagtg ttggtggcaa cgtgtttgcc
      961 gtgtgcgtac agctcttgcg ttgacggcag gcgttcctga tgggaggcgg tcaggctgag
     1021 tttgtggtgc ggcgtgaaat accagttgcc cgaaagtgcg aacgagcggg cggtttggcg
     1081 gtgtgcgccg aggtcgggca gggggtagtt gtagtggttt tcccgatcaa tcaatgcttt
     1141 gtcgtaccgg atggaggctt tttgtttttc cacgcgtacg ccgccttcaa gcgtgaagtt
     1201 gtcccagttt gcctgttcta caccgaaaaa gctgtaatgt tgcactttgt tgtcaagcag
     1261 catcggttgt ttgaccgctt cggatatggc agataaagca ctggattttt gttgtaaata
     1321 ttgcacgccc cagctgcctt tcagacggcc tatgggttgg tggcgcaact cgatacgggc
     1381 gttgtgtgtt ttgttgttga agaagttctc tactgcatcg cctgcttttt cgtcgtggtg
     1441 gtagtcattg cggttcagat gtacgcgcag ggcttcaaaa ccgggcagag gctgcttcca
     1501 ttcggcgcgg agttcgtagc gtttgttgcg caggtctatc cacggtttgc cgttgtgggc
     1561 atgggcgtgt gcattatcgt cgtcatgaaa gccgcagctc aagcccgggt tgtcgtaatc
     1621 gatgtcttct tcggtcagca ggtgcggata aagctgcaaa tagcgtttgt taatcaaact
     1681 cttttgccag atgatgtcgg cgtggcaatc atcgtattcg tggctgtggg caggcaggcc
     1741 gtattggtct cggcggtcgc tatacgccac gccgataaag cctttttcgc caacccaaga
     1801 cagcccgata ctgcccgttt gcgaatcggc gtggctgtcg ggcaggcgtt tcagattgcg
     1861 gtaacgcggt acggcgtaat cgcccgattt gcggtacagc ccttccgtgt gcaacacgaa
     1921 gtttttgccc agaccgacat tgatgccgcc ggacgtgagt ttttccagat tgccgctgct
     1981 caaacgcagc cccagttcgc ccgatacgcc gttttcaggc attttttcgg ggattttgcc
     2041 atcggcaaca tcgaccaaac cggccacatt gcccgaacta tataaaagcg taaccggccc
     2101 gcgcaggatt tcgacctgtt gcgacaaggc ggtatctacc ataatggcgt gatcgggcga
     2161 aaagtccgcc atatcgcctg tttcgccgtg atggttcaaa accttaatcc gcctgcccgt
     2221 ttgaccacga atgacaggag cggacgcgcc gccgccgtat tgcgaagcgt ggatgcccgg
     2281 aacgccgtcc aaagcgtcgc ccaagttgac ggctttttgg cgcaaggtat cgccggagat
     2341 gattttgtcg gaggcggtcg aagtgtgcaa cagccccgaa gtggcgtgcg gacggctttt
     2401 gccgacgacg ctgatcgttt ccaagcccac cgattgctca gttccatgcg cttgggcgag
     2461 gaggggtgtg ttgattaaaa gaattgataa aacaatgggt ttgagtgtgg tttgtgccat
     2521 tttagcttct cttcgcattt caaaattttg ttattatata acattacatt ctttatatca
     2581 taagattttg agaacactca gagggcatag gcaaaaattt ttcaaatgaa acggttgtga
     2641 tatttaatgg cgttaatttg tattaaccct aattttaggg aagttaatga tgtgaacacg
     2701 aacgccgcta tatttggcta tcggataacc aaaatttatg agggcataaa atcggcacgt
     2761 tcccgaacgg tcgaaaaggt ggcaaaatgg cgtacttgtc agaatgcgag gctctgcgcc
     2821 agattcctaa gggtgcatca ggcacgctaa gatataaggg gatatgctca ggggtatcgg
     2881 aaatgcagcc aataataaat aatattttaa atcaataggt taatcacaaa atgcttttgt
     2941 ttatcgacaa ttacgacagt tttacttaca acatcgtcca gtattttaca gaattggggc
     3001 aggaagttgt cgtgcgccgt aacgatgata ttacattgga agaaatcgaa gccttaaagc
     3061 cgcaatatct tgtaatcggc cccggaccgt gttcccctaa agaggcgggt atttcagtag
     3121 aagccatgcg ccattttgcc ggcaggctgc cgattatggg cgtgtgcctc ggacatcaga
     3181 cgataggcga agcgttcggc ggagatgtga tacgggcaaa aaccttgatg cacggcaagg
     3241 tgtcgcctgt atcccattcg ggtaagggta tgtttaaaga tttgcccaat cctgttacct
     3301 gtacgcgtta tcacagcctc gttatcgctc gggatacgat gcccgaatgt ttggaagtaa
     3361 cggcttggac tgaggacggc gagattatgg gcgtgcgcca taaggaatat gccgtcgagg
     3421 gcgtgcagtt ccaccccgaa gccctcttga ccgaacacgg acatgatatg ttgaacaatt
     3481 ttttaatcga atttcaaaac ttcaaaccgc aaaaaatctg acgtgatgcc gtctgaatcc
     3541 cttcagacgg cattttcgtc cgaatattga acggaggaca aaaaatgatt acaccgcaac
     3601 aggctatcga acgattaatc agcaataatg agttgtttta cgatgaaatg accgacttga
     3661 tgcgccaaat gatgagcgga aaaatgccgc ccgaacaaat tgcggcgatt ttgacaggat
     3721 tgcgtatcaa ggttgaaacc gtttccgaaa ttaccgcagc tgcagccgtc atgcgcgagt
     3781 ttgcgacaaa agtgccgctg gagaatgcgg aggggctggt tgatatcgtc ggtacgggcg
     3841 gggatggtgc caaaaccttc aatatttcga cgacttcgat gtttgttgcc gcagcggcag
     3901 gcgcgaaggt tgccaaacac ggaggccggt cggtctcttc ctccagcggt gcggctgacg
     3961 tggtggagca gatgggcgca aacctcaacc tgactcccga acaggttgcc caaagtatca
     4021 ggcagaccgg catcgggttt atgttcgcgc ccaatcacca cagtgccatg cgccatgtcg
     4081 cccctgtacg ccgttcgctc ggtttccgaa gtattttcaa catattgggt ccgttaacga
     4141 atcctgcggg cgcgccgaac cagcttttgg gcgtgttcca caccgatttg tgcggcatcc
     4201 tttccagagt attgcaacag ctcggctcca agcacgtttt ggttgtttgc ggggagggcg
     4261 gtttggatga aattacactg acgggcaaaa cacgcgttgc cgagctcaaa gacggaaaaa
     4321 tcagcgaata cgacatccgc ccagaagatt tcggtatcga aacccgccgc aatttggatg
     4381 aaatcaaagt tgccaatact caggaatctt tgttgaaaat gaatgaggtg ctggaaggaa
     4441 gagaaggggc tgcgcgcgat atcgtattgc ttaataccgc cgccgcccta tatgccggaa
     4501 atgtcgctgc ttcgctttca gacggcatat ctgccgcacg ggaagccatc gattcaggca
     4561 gggccaaatc gaaaaaagag gagtttgtcg gttttcaacc acaacaaaga tgccattttc
     4621 ttggaaagat ggagcttggg tgatgccgcc atgattatgg aacttttgtg gcaaaacata
     4681 agcacttcac gaagagaact taccaaactg tttttatata aaaacttggg gctgtactag
     4741 ataaccagac caaattccca ttaactaatt gtcttaaaat ctgaatttga gattctattt
     4801 aaaatgccat tggcatttct ttaaatgcag ccccaaatgc tctttgggaa tgccgttaaa
     4861 cttacgtaaa tggctaaatt cactaatatc aagcacatca taactacgaa aggtatccgt
     4921 gtatacaatg ccatcaggct taactttctt tcggataatt ggcaacaatg tcgctgattg
     4981 cgcattagga acaacgacgg tataaacctt atatattgcg tccctaagaa gggacgatta
     5041 acaaaaatta acgtccttta ctttctacaa gtaacagggc ttttttttgc ccgtttttga
     5101 ggattcgcac catggaagat aagcaaggga tgacaaaggc ggttgccggc gtgatgacgg
     5161 acgcgccgac ggacggcagg aagccggcaa ccgcttcaaa ccttcccccc ttatctaacg
     5221 ggggggtaca gaaaccgaaa cggcaggcag ggttcaggaa gtcttcgaat gttacgaaac
     5281 gtacataacg gacggtaaag gaaacctgtt aggcgttcct cttcggcgcg gtgtatcaga
     5341 ttcggctttc attgatcaaa t
//
LOCUS       DAWFAN010000135         5343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWFAN010000135 DAWFAN010000000
VERSION     DAWFAN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.135"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            42..515
                     /locus_tag="WGO65_001635"
     CDS             42..515
                     /locus_tag="WGO65_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ0327910.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            641..1744
                     /gene="mnmA"
                     /locus_tag="WGO65_001636"
     CDS             641..1744
                     /gene="mnmA"
                     /locus_tag="WGO65_001636"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ0327911.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGGGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYEGDVCLGGGIIQSTDKPVIITR"
     gene            1816..3486
                     /locus_tag="WGO65_001637"
     CDS             1816..3486
                     /locus_tag="WGO65_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEZ0327912.1"
                     /translation="MEKIWLDSYEQGVNAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            3598..5232
                     /locus_tag="WGO65_001638"
     CDS             3598..5232
                     /locus_tag="WGO65_001638"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ0327913.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPQLEKAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         1406 a         1452 c         1365 g         1120 t
ORIGIN      
        1 attaagcaca ttccccgtct gatattataa ggagcagaaa gatgaaaaaa ttattggcgg
       61 ccgtgatgat ggcaggtttg gcaggcgcgg tttccgccgc cggagtccac gttgaggacg
      121 gctgggcgcg caccaccgtc gaaggtatga aaataggcgg cgcgttcatg aaaatccaca
      181 acgacgaagc caaacaagac tttttgctcg gcggaagcag ccccgttgcc gaccgcgtcg
      241 aagtgcatac ccacatcaac gacaacggcg tgatgcggat gcgcgaagtc aaaggcggcg
      301 tgcctttgga ggcgaaatcc gttaccgaac tcaagcccgg cagctatcac gtgatgttta
      361 tgggtttgaa aaaacaatta aaagagggcg acaagattcc cgttaccctg aaatttaaaa
      421 acgccaaagc gcaaaccgtc caactggaag tcaaaaccgc gccgatgccg gcaatgaacc
      481 acggtcatca ccacggcgaa gcgcatcagc actaatctgc tgaaaatgtt tgaaatgccg
      541 tctgaaaaag cccgggcttt cagacggcat ttttatgact gcctttaaaa tgtgttaaaa
      601 tccgccttta aaaaccgccg tttccaacca tattctacgt atgaatacga cagcaaaccc
      661 ttccaatatc atcgtcgggc tctccggcgg tgtcgattct tccgtaaccg ccgccctgct
      721 caagcagcag ggttatcaag tgcgcggtgt gttcatgcag aactgggaag acgacgacaa
      781 cgacgaatat tgcagcatca aacaggattc gtttgatgcc atcgccgttg ccgatattgt
      841 cggcatcgac atcgacatcg ttaatttcgc cgcgcaatat aaagacaaag tctttgctta
      901 ttttcttcag gaatacagtg cggggcgcac gccgaatccc gatgtgttgt gcaacgccga
      961 aatcaaattc aaatgctttt tggattacgc cgtagggcag ggcgcggata ccatcgccac
     1021 cggacattat gcgcgcaaag aagtccgcaa cggcgtgcat tacctgctca aaggtttgga
     1081 tcgaaacaaa gaccaaagct atttcctcta ccgcctcaag cctttccaac tcgagcgcgc
     1141 gatttttccg ttgggcggtt tggaaaaacc tgaagtccgc cgccttgccg ccgaatttaa
     1201 tttgccgacc gccgccaaaa aagacagcac gggcatctgc ttcatcggcg agcgtccgtt
     1261 ccgcgagttc ctgcagaaat acctgccgac cgataacggc aaaatggtta cgcccgaagg
     1321 gaaaaccgtc ggcgaacacg tcgggctgat gttttacaca ttgggtcagc gcaaaggttt
     1381 gggcatcggc ggcgggggcg aaccgtggtt tgttgcggct aaagatttga cgaaaaacga
     1441 actgattgtc gtgcaaggac acgaccatcc gctgctctat acccgcagcc tcgtcatgaa
     1501 tgatttgagt ttcacgctgc ccgagcgtcc gaaggcagga cgctatacct gcaaaacgcg
     1561 ttaccgtatg gcggacgcgc cttgcgaatt gcgctatttg gatgatgaaa ccgccgagct
     1621 ggtgtttgac gaaccgcaat gggcggtaac gcccggtcaa tccgccgtgc tgtatgaggg
     1681 cgatgtctgc ttgggcggcg gcatcatcca atcaactgac aagcctgtca tcatcacgcg
     1741 ataaaggtaa tgccgtctga aacggttttc agacggcata gttctgcttc atttcattct
     1801 aaaagaccga aaattatgga aaaaatctgg ttagacagtt acgaacaggg cgtgaatgcc
     1861 gaaatcgaca tcacgcaata cggttccgtc agcgatgtat tttgccaaag tgtcaagaaa
     1921 tttgccggcc aacctgcttt tcaaaacatg ggcaagacgt tgacttatgc cgaaaccggc
     1981 aaactggcga ccgatttcgc ctcttatctg caaaacgtcc ttaaattgcc tcgaggagag
     2041 cgcgtggcaa taatgttgcc caatgtactg caatacccgg ttgcgctttt cggcattttg
     2101 caggcaggtt tggtggcggt gaacaccaat ccgctctata ccccgcgcga gttggagcat
     2161 cagttgaaag acagcggtgc gaccgccatc atcgttttgg aaaatttcgc caacacgctg
     2221 gagctggtgc tgccgcgcac gcagatcaaa cacgtcatcg tcgcctccgt cggcgaaatg
     2281 ttcgggctgc ttaaaggttc gctgatcaat ttcatcatcc ggaaaatcaa gaaaatggtg
     2341 cccgaatacc gtattcggga aaccgtttcc tttcagacgg cattgaaaga gggggcgaag
     2401 cacgttttcc aacctgtcgc attaaaccgc gaagataccg cgctgttgca atacacgggc
     2461 ggcacgacag gtgttgccaa aggggcggtg ctgagtcacg gcaacatctg cgccaatatg
     2521 cttcaggcaa aagaatggat taaaaaccag ttgcgcgagg gcaaagaaac cgttatcgcc
     2581 gccctgccgc tgtatcatat ttttgcctta accgtgaatc tgatgatttt tgccaatgcc
     2641 ggctcgaaaa tcatcctgat taccaacccg cgcgatatga aaggctttat cggagaactg
     2701 aaaaaacagc gggttaatgt atttatcggc gtgaacacgc tgtttaacgc gatggttaac
     2761 cgtcctgatt ttgccgaagt cgatttttca ggattgcggc tgactttggg cggcggtatg
     2821 gcgacccaaa aagccgttgc cgaaaaatgg aaaaaaatca ccggcacgcc catcgtcgaa
     2881 gcctacggtc tgaccgaagc cagccccggc gtgtgctgca accccttaaa catcgaatca
     2941 tacagcggca gcatcggttt gcccgtctcg tccaccgaag tcgaactgcg cgacgcaaac
     3001 ggcaaagaag tccccgtcgg gcagccgggc gaattgtggg taaaaggccc tcaagtgatg
     3061 caaggctact ggaaccgccc cgaagaaacc gccaaagcca tagacgcgcg cggcttcttg
     3121 gaaactggcg atattgccgt catggacgaa aaaggctggt tgaagctggt cgatcgtaag
     3181 aaagacctcg tcgttgtttc cggattcaat gtttatccga acgaaatcga ggaagtcatc
     3241 gcgcatcacg gcaaagtgat ggaagtcgcc tgtatcggcg tacccgacga aaaaaccggc
     3301 gaggcactca aagtattcgt cgtcaaaaaa gacccgtcat tgaccaaaga agaacttatc
     3361 gccttttgcc gcaccgaatt aaccgcatat aaagttccga aaaatatcga attccgcgac
     3421 gagttgccca agtccaacgt cggcaaaatc ctgcgccgcg agttgcgcca aagtaccggg
     3481 aaataaagaa aagatgccgt ctgaaaacag ccgtccaccg ttcagacggc atccgtccgt
     3541 ttgccagaac cgcgcgcttc acgttaaaat cacgcattcc aatatgggta ttccatcatg
     3601 accaaattca ttttcgtaac cggcggcgtt gtctcctcac tgggtaaagg tatcgccgcc
     3661 gcttctattg ccgccatcct cgaatcgcgc ggcttgaacg ttaccatgct caagctcgat
     3721 ccttatatca acgtcgatcc cggcacgatg agcccgttcc aacacggcga agtgttcgta
     3781 accgacgacg gcgcggaaac cgacctcgac ttgggacact acgaacggtt tatcgattcc
     3841 acgatgaccc gccgcaacag cttcagcacg ggacaggtgt acgaaaacgt tatcgccaaa
     3901 gaacgacggg gcgactattt gggcggcacg gttcaagtca ttccgcacat taccgacgaa
     3961 atcaaacgac gcatccatga aggcgcggca ggttacgatg tggcgattgt cgaaatcggc
     4021 ggcacggtcg gcgacatcga atcgctgccg tttttggaag ccatccgcca gatgcgaagc
     4081 cagttgggac gcaacaacac cctgttcgcc cacttgagct acgtccccta catcgccgct
     4141 gcaggcgaaa tcaaaaccaa gccgacccag cacaccgtta aagaaatgtt gagcatcggt
     4201 ttgcaacccg acatcctgat ttgccgtatg gacaggacaa tgcctgcgga cgaacgccgc
     4261 aaaatcgcct tgttctgcaa cgtggaagaa cgcgcgattg tcggcagcta cgatgtggac
     4321 agcatctacg aatgccccga aatgctgcac gaccaaggca tcgataacat cattaccgag
     4381 cagttgcagc ttaatgtgca gcaggcggat ttgaccgcgt ggaaaaaaat cgtccacgcc
     4441 atccaaaacc cgaaacacac cgtcaaaatc gccatggtcg gcaaatacgt cgatttgacc
     4501 gagtcctaca aatcgctgat cgaagccttg aaacacgcag gcattcacac cgaaaccgac
     4561 gtacaaatta cctttgttga cagcgaaagc atcgagaaaa acaacggcga cgtttctatg
     4621 ctcaaagata tggacgccat cctcgttccc ggcggttttg gttcgcgcgg cgtggaaggc
     4681 aaaatcgccg ctgtgcgcta tgcccgcgaa aacaacgtgc catacttggg catctgcctc
     4741 ggtatgcaga ttgcgctgat cgaatacgcc cgcgacgtgg caggtttgaa aggtgcgaat
     4801 tccaccgagt tcgacctcaa atgcgccgcc cccgtcgttg ccctgattga cgaatggcaa
     4861 accgccgacg gcagcgtcga aacccgcgac gaatccgccg atttgggcgg caccatgcgt
     4921 ttgggcgcgc aagaagtcga attgaaagca ggcagcctcg ccgccaaaat ctacggcagc
     4981 ggacacatcc gcgaacgcca ccgccaccgc tacgaagtca acaacaacta tgttcctcag
     5041 ttggaaaaag caggcttggt tatcggcggc gtatccgccg gacgcgaacg cttggtcgaa
     5101 accatcgaac tgccgaacca tccttggttc ttcgcctgcc agttccaccc cgagtttacc
     5161 tccaatccgc gcaaagggca tcctttgttc accgcgtttg tcaaagctgc gttgaacaat
     5221 aaaaaagcct gataaagcgt tacttgatga tcaaaatgcc gtctgaaagc ctgacaaaga
     5281 ccttcagacg gcattttcgc aagtcgggga ttataaaagc catcaattta atttttatgg
     5341 ttt
//
LOCUS       DAWFAN010000136         5335 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWFAN010000136 DAWFAN010000000
VERSION     DAWFAN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.136"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            295..1728
                     /locus_tag="WGO65_001639"
     CDS             295..1728
                     /locus_tag="WGO65_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ0327914.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVGNPATGETLAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLIKENKEKLARIMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALVVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALLKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1837..2625
                     /locus_tag="WGO65_001640"
     CDS             1837..2625
                     /locus_tag="WGO65_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ0327915.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLEVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            2993..4549
                     /locus_tag="WGO65_001641"
     CDS             2993..4549
                     /locus_tag="WGO65_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0327916.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPAVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4566..5312
                     /gene="surE"
                     /locus_tag="WGO65_001642"
     CDS             4566..5312
                     /gene="surE"
                     /locus_tag="WGO65_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ0327917.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
BASE COUNT         1246 a         1342 c         1525 g         1222 t
ORIGIN      
        1 gtttggattt ttgccatact ctaccacacg ttgcaactgc aatctttgtt ccttatatat
       61 tattaaaggg ctgcattact tttctttcta aattaccatt gtataaaaag tatttttctt
      121 aaacgacagg gattgcgccc gcgccatatt cagacaccgc ccggatgttg cgctgcccga
      181 tcggatgctt cagacggcat cggaagggtt tgcagttttg gaatatgaag atgataatgt
      241 ccgcgagatt gacggcattt gaaagcggtt attccgcata ccggaggata cgaaatgaac
      301 gaatattacc aattaatcaa gcatcccgat atttcccttt ccccgatttc agacggcatc
      361 ggggtcggca atccggcgac gggcgagact ttggcgtatg tccgcaatac gggttcggac
      421 aaactgaaaa acctgattca aaaagcagct gcggcacaaa aattatgggc ggcaaaaacc
      481 gctttggaac gcgccgacat attgtggcgt tggtattttt tgattaaaga aaacaaagaa
      541 aaactggcgc ggatcatgac gatggagcag ggtaaaagcc tgactgagtc gcgtggtgaa
      601 atcgattatg cggcttcgtt tgtgcgctgg tttgccgaag aagcgcggcg gattgacggc
      661 gatgtgctga cgagtgtgaa agcgtcgcaa aaactggttg tgttgaaaca gcctgtcggc
      721 gttaccgctg cgattacgcc gtggaacttc ccatctgcaa tgattgcgcg caaggctgcg
      781 cctgctttgg cggtgggctg cgcgatgatc gtcaaacccg catcgctcac gcctttaagc
      841 gcgtatgcgt tggtcgtgct ggcttatgaa gcgggcgtgc cgcaggattt gctgcccgtc
      901 gtcagcggca gtgcatcgga aatcggccgt gaatttgcca cgaacccgat tatccgcaaa
      961 atcagcttca ccggctctac cgaagtcggt gcgaaaattt ttgccgacag cgcggcggac
     1021 attaaaaaac tcagtttgga gctgggcggc aacgcgccgt ttatcgtgtt tgacgatgcc
     1081 gatttggaca aagccgtcga aggtgcgctc gccagcaagt tccgcaacag cggtcagacc
     1141 tgcgtctgca ccaaccgcgt ttacgcccaa tcgggcattt acgacgcatt ttgccgcaaa
     1201 ttgagtgaaa aagcagccgc gctcaaattg ggcaacggct tggaggatgg cgtaaaccaa
     1261 gggccgctga ttgaggaaaa agcggtggag aaagttgagc agcacatcgc cgacgcgctc
     1321 ttaaaaggcg cggtctgtct gaccggcggc aaacgcagcg cgttgggcgg aacgtttttt
     1381 gagccgaccg tcttaagcgg cgtaacggcg caaatggcgg tagctcgcga ggaaaccttc
     1441 gggcctttgt gtccggtatt ccgttttgaa accgaagccg aggtcatcga ggctgcgaac
     1501 aatacggaat acggtttggc agcttacctt ttcaccgccg acaccgcccg ccaatggcgc
     1561 gtcggcgaag ccttggaata cggcatggtt ggcatcaata cgggcttaat cagtaatgaa
     1621 gcggcaccgt ttggcggcgt gaaacgcagc ggtttgggac gtgaaggcag caaatacggt
     1681 gcggacgaat atctggaatt gaaatatctg tgtatggatg tcgggtgacg gatgccgtct
     1741 gaacggcagg gtttcagacg gcgccgcatt ttaagcagtc tctatctgtt gtacaatgcg
     1801 ccctgttttt acggttattt ttgatttgaa caagatatga tggaaaacgg aaaaacagtc
     1861 ccgagatggc gttttgcctt gaaaagtgcg ggctggcatc tcttaatcag cctgtcggtt
     1921 gcagggctgg cggcattgct ggtttttaag gtttggtatc cttatcctta tgccgagctg
     1981 acgggagggc tgtcgcttta tcagctggtg gtggctgtcg atattgtatg tggtccgctg
     2041 ctgactttaa ttttggcaag cccgaagaaa aagacaaagg cacgcatggt cgatttttcc
     2101 atggtcggca tcatccagct ggcggctttg gtgtacggtc tgcacagcgt ttctttggcg
     2161 cgtcccgtgg tggaggcgtt tgaaaaagac aggattaatg ttgtgaccgc cgccgaagtg
     2221 gcggttgaag acctgcacaa agcgccggaa gggctgcaaa gcctgtcgtg gttcggcatc
     2281 cgccgcattg cattgaaaga acctgaggat gcggatgaga agaacaagac gctggatttg
     2341 tccctgaaag gtatcgagcc gagtatgcgt cccgaccggt ggctgccgta ttccgacaag
     2401 gaagcagaag aaatccgcaa acatctgaaa ccgctggaag tcttggcgga tgcgagaaaa
     2461 acgacggttg cggacattct gaaacaggca ggtctcgccg aaggggagga gctgtattac
     2521 ctgccgttta ccagcagcag gcagaaagag tggatagtca ttaccgataa agagggcaac
     2581 accaaaggtt acgcgccgat agacggcttc atcattaccc cttaagcgtt gggactccgt
     2641 ccgcactcga acatccgttc ttcgcggcgg tagaatcaga ctgtatttga gaggggcgca
     2701 acccctcaat cagggcaaca cataggggaa ggctttatgt gccgtccgtg tgttgaaaca
     2761 tttcaaaata agctcatgcc gtccgaacat cctttcagac ggtatggcgt tttgccattg
     2821 ccccgataag ctgttaaact atttaaatta tttcgaccga aggtacacgc acatgcaaga
     2881 ccccgagcag agcagcaagc ccgcccgccg ttttttgtac gtaccgtctg ttcaggcagc
     2941 ttgagcgctt caggctgcct tttcgcgttt gaacttgagg aatacgaaaa tcatggattt
     3001 tagttggttg gcagaaccgc atacctggat aggttttgcc acgcttttgg tgttggaagt
     3061 cgtattgggg atagacaatc ttgtctttgt ggcgattttg gcaaacaagg tccagcccgc
     3121 acggcgcgac cgcgcacgga ttatcgggct ggggctggca gtcgtcatcc gcatcattat
     3181 gcttgctttt atggcgcaca tcatcacgct gaccgagccg ctgttccaaa tcggcggcct
     3241 cgccgtttcc ggcaaggaca tgattatgct cgcgggcggt attttcctgc tttacaaagc
     3301 caccaccgaa ctgcatgaac gcctcgaagg gcacaaccgt tttaccgttg ccgacagcca
     3361 aaaaaaacac gcgccgtttt ggggcgtggt cgcgcaaatc ctgatactgg atgccgtgtt
     3421 ttccatcgat tcggtcatta ctgcggtggc gatggtcgat catatcgtcg tggcgatggg
     3481 tgcggtcgtc gtcgcaatgg ctgtaatgat ttctgccagc aaactcttga ccgaatttgt
     3541 ggacaggcat cccgccgtcg tgatgctctg ccttggtttt ttgttgatga tcggtttcag
     3601 cctgattgcc gaagccttcc atttccacat tcccaaaggc tacctctacg ccgccatcgg
     3661 cttctcgatt ttaatcgaat tgtttaacca gatttcgcag cgcaacagcc gcaaaaacga
     3721 ctacatcggc agctcgtggc gcaagcgcac cgccgaaaac gtcttgggta tgatgggcat
     3781 acgcgaaagc gtgcttgccg atgcgggcgg cgaatccggg gacgacgcgc attttgaaga
     3841 aaacgaaaaa tcgatgatac gcagcgtgct gacgcttgcc gaacgcccga ttatgggggt
     3901 gatgatccca cgccgcgaca tcgaacggct ggacatttcc caaagccgcg aagaacagtg
     3961 tgcccaactg caaaacacgc cttacagccg cctgctcgtt gtcggtaagg cgggcgtgga
     4021 cgaacctttg ggctacatca acaaaaaaga cctgctgtcc caactgctgg aaacaggcgg
     4081 tctcgacatt cagacggcat tgcgccagcc gctcgttttg cccgacagca ccaccgcgct
     4141 gggcgcaatc gaactcttcc gccaaagcag cgcggattac gccctcgtcg tggacgagtt
     4201 cggcgcggta ttgggcatgg taaccatgaa agacctgctc gaaaccatcg caggcgagtt
     4261 ccccgaagaa tttgagcgcg aagaagaacc agccgttcag gggaatcccg atgaaagcct
     4321 gacggtggaa ggcgcgttgg aatatgtgga actcgcaccg caactcaacc tgccgcagca
     4381 ggaggaagat gccgatttcc atacggttgc cgggctgatt atggaagaat tgcaaaccat
     4441 ccccgatgtc ggtgattttg ccgatttcca cggctggcgg tttgaagtgg tcgaaaaaga
     4501 agggcagcgc atcgagcggg tcaaaatcac caaattgccc gaagaataag cattcaggat
     4561 agaaaatgaa cgttttgatt tccaacgacg acggctacct ctccgaaggc atcgccgttt
     4621 tggcgcgcgt tacggcggaa tttgccaacg tcagggtggt tgcgcccgaa cgcgacagga
     4681 gcggggtcag caattcgctg acactggaac gccctttgca gttgaaacag gcgcaaaacg
     4741 gcttttacta tgtcaacggc acgccgaccg actgcatcca catcgggcag tctgtatttt
     4801 cggattttca ggccgatttt gtcttttcgg gcatcaaccg gggcgcgaat atgggggacg
     4861 acacgcttta ttcggggacg gttgcggcgg caaccgaagc ctaccttatg ggcatgcccg
     4921 ctgtggcgtt ttccttaaac gacgcttccg gacgctattg ggcaaccgca gaaaaggcac
     4981 tgtggacatt gttggcgcat tttttcaaaa accccccgca gtcccctatt ttatggaaca
     5041 tcaatatccc cgccgtcgcg ccggaagatg tgcggggcat taaaatcgcc cgtttgggca
     5101 ggagacacca cggtcagaac gtcatccccg cgcgcaatcc gcgcggcgaa cagatttatt
     5161 ggatagggcc ggtcggcgaa gtttccgatc gggaagaggg aacggatttc ggtgaatgcg
     5221 gcgcaggttt cattaccgta acgccgttgc aaatcgacct gaccgcctat ccggacatgg
     5281 cggaaacggc ggcgttctgg catacggact gaccgtttca tcaaatatag tggat
//
LOCUS       DAWFAN010000137         5270 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWFAN010000137 DAWFAN010000000
VERSION     DAWFAN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.137"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            113..808
                     /gene="pgl"
                     /locus_tag="WGO65_001643"
     CDS             113..808
                     /gene="pgl"
                     /locus_tag="WGO65_001643"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ0327918.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            789..1775
                     /locus_tag="WGO65_001644"
     CDS             789..1775
                     /locus_tag="WGO65_001644"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ0327919.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1825..2673
                     /gene="hexR"
                     /locus_tag="WGO65_001645"
     CDS             1825..2673
                     /gene="hexR"
                     /locus_tag="WGO65_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ0327920.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2794..4440
                     /gene="pgi"
                     /locus_tag="WGO65_001646"
     CDS             2794..4440
                     /gene="pgi"
                     /locus_tag="WGO65_001646"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0327921.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGSQKHDSSTERLINLYLQTNRK"
     gene            4454..5005
                     /locus_tag="WGO65_001647"
     CDS             4454..5005
                     /locus_tag="WGO65_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0327922.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSELACKTVETEARLEELY
                     NRKKALIDEMVREADKKELPKRL"
     gene            5050..5190
                     /locus_tag="WGO65_001648"
     CDS             5050..5190
                     /locus_tag="WGO65_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327923.1"
                     /translation="MPGYRNWQNGLNAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
BASE COUNT         1337 a         1467 c         1399 g         1067 t
ORIGIN      
        1 aaagcagtcc cgatatttgg tatcaaaagc ataaacaact gttcggccgg catattgctc
       61 aacccatttc cataacggaa tatgccgtct gaaataaaaa aaggacacaa atatgtttgt
      121 ttggcacgaa tacgaaaatg cggcagaagc ggcgcagtct ttggctgacg cagtggcgga
      181 tgctttgcag ggcgcactgg acgagaaggg cggtgcggtg ttggcagttt ccggcggacg
      241 ttcgccgatt gcatttttca acgccctgtc gcaaaaagat ttggattgga aaaacgtcgg
      301 catcaccttg gcagatgaac gcatcgtgcc gaccgtccac gccgacagca ataccggttt
      361 ggtgcgcgaa tacctgttga agaacaaagc ggaagcggca atgtggattc ctatggtgga
      421 agacggaaaa actgaaaccg aattacatcc cgatgctgtt gtcgattatg cactgaaaca
      481 ttacaaacag cccgatgttt tggttttggg tatgggaaac gacgggcata cggcttcgat
      541 tttcccgaaa gctccgcagt ttcagacggc aatcgacggt tcggcaggtg tcgcgctggt
      601 gcataccacg cccgttaccg cgccgcacga gcgcgtcagt atgaccttgg atgcgattgc
      661 ccatacgggg catgtgtttt tggcgatacg gggcgaagag aaaaaagccg tgttcgacca
      721 agccgcacaa ggcgaaaacc gcgaatatcc gatcaacctc gttttgaacc atcaaggagt
      781 gaactgccat gtcttctacg ccgaataaac aagccggata tccccgactg gtcgccgata
      841 tcggcgggac gaatgcacgc tttgcgctgg aaaccgcgcc gcgcgtcatt gaaaaagccg
      901 ccgtgcttcc gtgtaaagac tacgatacgg ttaccgatgc ggtgcgtgcc tatctgaatc
      961 aaagcggtgc aacagccgta cggcacgcgg catttgccat cgccaacccg attttgggcg
     1021 actgggtgca gatgaccaac caccattggg cgttttccat cgaaaccacc cgtcagactt
     1081 tggggctgga caccctcatc cttttgaacg actttaccgc gcaggcattg gcggtaacgc
     1141 agacttcaag caaagacctg atgcaggtag gcgggcaaaa gcctgtcgaa tttgccccca
     1201 aagccgttat cggccccggt accggcctgg gcgtgagcgg attggtgcac agccacgcag
     1261 gctgggtggc tttggcaggc gagggcgggc ataccagttt cccgccgttt gacgatatgg
     1321 aagtgctgat ttggcagtac gccaaaaaca aatacggcca tgtttccgcc gaacgctttt
     1381 tgagcggcgc gggcttgagc ttggtttacg aggctttggc tgcaaaacag aaagccaaac
     1441 ccgccaaact gatgccgtct gaaatcacgg aaaaggcttt gagcggcgcg tcgcctttgt
     1501 gccgtcagac tttggacatc ttctgcgcca tgctcggcac ggttgcttcc aacctcgccc
     1561 tgacgctggg tgcgcgcggc ggcgtgtacc tgtgtggcgg cattattccc cgcgtgttgg
     1621 aatatttcaa aacttccccg ttccgcagcc gtttcgagaa caagggcagg tttgaagcat
     1681 atcttgccgc gattcccgtg tatgtcgtct tgagcgagtt tcccggaatt tccggtgcgg
     1741 ctgcggctct cgacaaccat ttgagaaacg tttaaccaca gcggctcctt gcagcggggc
     1801 tgcattatcg aagggcatat cattatgtta agcaaaatca gcgaatcact ggcaaacctt
     1861 tccggtgcgg aacgcaaagt cgccgaatgt gcattggcgg aacccaaatg gttcgtccat
     1921 gccgccgttg ccgaaattgc cgaacgtgcg tccgtcagcc agccgaccgt catccgattc
     1981 tgccgcagct tgggttataa agggctgccc gagttcaagc tcgccttgtc cgccagcatc
     2041 ggtcatgagg gtatgcccta tgtccacgaa gaactcaacg ccgacgacga tatggcaagc
     2101 gtggtcgaga aagtattggg caatgccgcc gcctcgctgt tgggcgaacg ccgcttcctg
     2161 aaagagtcgg agctggaaaa cgccattgcc acgctgatgc acgcccgccg cgtcgagttt
     2221 tacggtgtcg gcaattccgg tatcgtcgcg caggacgcgc agcacaagtt tttccgtttc
     2281 ggaatgtcca cggtcgccta tgtcgatacg cacacgcagc tgatggcggc atccgttttg
     2341 agcgatcagg atgttttggt tgccatttcc aacacgggtt cgtctatcga acttttggat
     2401 gcggtcagca tcgccaaaga aaacggcgcg tccgtcatcg cactgacccg caacgattcg
     2461 cctctggcgc aacttgccga ctgcgtgttg agcgttgcca cacaggaaaa tgccgaactc
     2521 tacacgccca tggtttcccg cctcttgcag cttgccgtca tcgacatcct tgccatcgga
     2581 cttgccctgc gcttgggcga tgctgccagc ctgcaattgc agaaaagcaa aaaaagcata
     2641 cacaacaagc acatcgatta cgacaaagat tgaccttcag acggcatccc acaaatgccg
     2701 tctgaaatgc cgaacaacgg tcgtcggcgg cttgcggcag tttccggcag ccttttcatc
     2761 ccacaacaaa aaacctcatt caggagcata tagatgaaac accttcacga cttacccgca
     2821 tggtcgaaat tgtggaacca ctttgacgac agcaaaacat tgcatatgcg cgaaatgttc
     2881 gagcaagacc cgcagcgtgc ggaacgctac tggctgcaag tcggcggact gacgctggac
     2941 tactccaaaa accgcatcaa cgacgaaacc atgtcgcttt tgttcgagct tgcccgagaa
     3001 gcaggcgtgc cggagcggat gcggcagatg ttccacggcg aaaaaatcaa taccaccgaa
     3061 aaccgcgccg tcctgcatgt cgcccttcgc aaccgcacca attcgccgat tgtggttgac
     3121 ggtgaagatg tgatgcccaa agtcaaccgc gttttgcaac gtatgggcga atttgcacac
     3181 gaagtccgca gcggaagctg gctgggctat accaaccaag tcattaccga cgttgtcaac
     3241 atcggcatcg gcggatcgga tttgggtccg ctgatgatgt gtaccgcgct caaacctttc
     3301 ggtcatccgc gcctcaatat gcacttcgtc tccaacgtgg acggctcgca actgcgcgac
     3361 gtattgtcca aagtccaccc cgaaaccacg ctgttcatca tcgcctccaa aacatttacc
     3421 acgcaggaaa cgctgaccaa cgcgctgacc gcgcgcgaat ggtttttgaa tcatgcgggc
     3481 gacgaagaag ccgttgccaa acacttcgcc gccgtttcca ccaatcaaaa agccgtcgcc
     3541 gaattcggca tcgacaccgc caatatgttt gaattttggg attgggtcgg cggtcggtac
     3601 agcctgtggt ccgccatcgg attgccgatt atgctgtatc tcggcgaaga aaacttcatc
     3661 gaaatgctca acggcgcgca cctgatggac caacacttca tcaacacacc gctcgagcgc
     3721 aacctgcccg tcattctcgc cctcatcggc atctggtata tcaactacta cggcggcggc
     3781 agccacgtca tcgcgcctta cgaccaacat ttgcaccgcc tgcccaaatt catccagcag
     3841 ctcgatatgg aaagtaacgg caaacaggtt acgttggacg gcaaagcagt cggacacgaa
     3901 acctcgccga ttatctgggg cgaaacgggc attaacggcc agcacgcctt cttccaactg
     3961 ctgcaccaag gcacgcacat tacccccatc gacctgattg cctcgcttga aaaacgcagc
     4021 aacctgcccg gacaccacga aatcctgctt gccaacgtct tcgcccaagc agaagccttt
     4081 atgcgcggca aaacccccga cgaagtccgc gccgaactca aagcgcaggg tatggatgag
     4141 gcgcgcatcg aagagctggt cccgcacaaa accttctccg gtaaccgccc gaccaacctc
     4201 atcctgatgg acaaggtcaa cccgcgtaat atgggcagcc tgattgccat gtacgaacac
     4261 aaaaccttcg tacaaggcat catttggggc atcaacagct tcgaccagtg gggcgtggaa
     4321 ctcggcaaac aactggctaa aaccattttg ggcgaactga ccggcgaaac cgggtcgcaa
     4381 aagcacgaca gctcgaccga acgcctgatc aacctctacc tgcagaccaa ccgcaaataa
     4441 aacctgcgga aaaatgccgt ctgaacgccg accgtttcaa acggcatttt tatcgaacag
     4501 gaaaaccgtc ggtaaacttg cagagcgtgt gcaatcccga tatgatggtt tgcataaatt
     4561 taaacatata tgttccgcag ctatggcact gattaaagag ccgttggaca aagcgaaaca
     4621 aaggaacgaa gaacttgaag cggcagaaga agcggcggcg caggaggcat tgggtcggga
     4681 gcaggaagcc gcccgcgtat ccgaatggga agaacgctac aagttgtcgc gcagcgagtt
     4741 cgagcagttc tggaaaggat tgccgcaaac tgtacagaat aaactgcaag cttcgcagaa
     4801 aacatggaaa agcggtatgg acaaaatctg tgccaacaat gcgaaagccg aaggtgaaac
     4861 gccaaacggc ataaaagtca gcgaactggc atgcaaaacg gtggaaaccg aagcacgctt
     4921 ggaagagctg tacaaccgta aaaaagccct tatcgacgaa atggtcaggg aagcggacaa
     4981 gaaagaactg ccaaagcggc tctgaacagc gcggttcagg cactgcccgc cgatattgcc
     5041 gaaaccgtca tgcccgggta ccgcaactgg caaaacggtc tgaatgccaa atgcgctgac
     5101 ggcgacgaat acggcgtggc acaatccgac tgccgtacca gagaaatcaa tgcgaaaacc
     5161 aaagaaatcc aaggttatct gattgactga aacttggatg cgggaatgtc ggcggctttt
     5221 gcgtttttgt cgtttttata gtgaattaaa tttaaaccga tacatcgttg
//
LOCUS       DAWFAN010000138         5222 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWFAN010000138 DAWFAN010000000
VERSION     DAWFAN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.138"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(102..2936)
                     /gene="lbpA"
                     /locus_tag="WGO65_001649"
                     /gene_synonym="tbpA"
     CDS             complement(102..2936)
                     /gene="lbpA"
                     /locus_tag="WGO65_001649"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ0327924.1"
                     /translation="MNKKHGFQLTLTALAVATAFPAYAAQAGGAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDIQSQDKTGQFFSVEGERES
                     KPVAKLAGYGKYLNNQLNRWVKERIEQNQPLSAEEEAQVREAQVRHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKF
                     RDKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISEST
                     GFDEKNQDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPDSAGRLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2933..5065)
                     /locus_tag="WGO65_001650"
     CDS             complement(2933..5065)
                     /locus_tag="WGO65_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0327925.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPATFKSK
                     DVPTSPPAGPSVETTPVNQPAVGAAMRLPRRNIAAFDKNGNEIPNSKQAEEYLPLKEE
                     DILFLDGTPKEQADKLKKEINGRYPNVKIYTSDLKDAVYKYEHVRAGYVYTRFGTDEI
                     EQNSGGKQVTHRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDVKRHRAGKAVG
                     IDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDQKTLNGKLIKNQYVQRKTD
                     EKKPLTIYDITAKLDGNRFTGSAKVNPDLAKNPAGNERLFFHADADQRLEGGFFGDNG
                     EELAGRFISNDNSVFGVFAGKQKTDASNASDTNPALPSGKHTKILDSLKISVDEATDD
                     HARKFAISTMPDFGHPDKLLVEGREIPLVSQEKIIELADGRKMTVRACCDFLTYVKLG
                     RIKTERPAVQPKAQDEEGDEEDTGVDSVEEGEDEIDDEDEVSEEDAEEIEEEADEVEE
                     EEAEADEAEETEEESPTEESGSGSNAILPAPEAPKGRDIDLFLKGIRTAEADIPKTGK
                     AHYTGTWEARIGVPDKKDDQPGTTFIQKDSYANQGAKAEFDVDFGAKSLSGKLTEKND
                     THPAVYIENGVIDGNGFHATARTRDNGIDLSGQGSTSPQSFKANNLLVTGGFYGPQAA
                     ELGGNIIDSDRKFGAVFGAKKDNKEVEK"
BASE COUNT         1126 a         1414 c         1223 g         1459 t
ORIGIN      
        1 gaaatcttgt ttatatcgaa tcaaaaaaac ctgcacctta atcagttggc ggtttagtcc
       61 gacttttggg gtgcagatca agctttcaga cggtatttcc tttaaaactt catttcaagc
      121 gcgaggctga agtttctgcc cggtgcggca taccttccat agttgctgtc gccgccgtgc
      181 cggtttgccg tgctttccgc agtctggcgc aaggattccc aagtaacgta gcggtagttg
      241 ccgatattgt agatagccgc cctcaaggtc agccgttttt tcagattcag ataggcggaa
      301 acgtctgccg tcgaccaaga agacgacgct ctttttgtcg aatatcgttt ttgatcgcct
      361 gccagataag caagctcgtc agggtttttc cctttggaat aggtcagcat aatgtttgcg
      421 ccccatttcc cctcgggctg gtcgtatccg aaccccaaaa cataacgcga cggctgtacc
      481 gcatccaaag catagctgcg gagggacaag tccggccggt tggataccga tttcggtttg
      541 atacggttgt acgccaaagt cgtgtacaaa ccttcgggca gtttgccgta cacgccgttc
      601 cagtcgattt ttcccaatat attaacgcct tgaagcgaca tattttgggc attgtaataa
      661 tcgcgtatat caatctctgt caatcgtcct gctgaatccg gcaatttggt tttgtgatcg
      721 gcaacggcaa tcatatcggt ataacggttg cggaagctgc tgatttccaa aaagccgaaa
      781 tcgcccttcc actgcaaacc gatttcgcgg ttggctgcct tttccgattt cagggcggga
      841 cgctgccagc ctttcggata atcgtgataa atgtctatcc cgaaaagttc ttggaatgag
      901 ggcgttctga agccgctgga ggcgcggtaa gacacggaaa aatgccggtt cggtttgaac
      961 acgatgccgc tgttccacga acggtcaaca taccgcccgc tgcggacgag ttcttccgac
     1021 gtggtgaagt ttttccggtc gtacctgccg cccaagctga aatcaaaata tttgccgatt
     1081 gaaaaacgat cgttcaaaga aatgtggata ttgctgccgt tgatttttct tggcacgcat
     1141 ttgcgggaac gcagggtttc gatgtagccg cagaccgatc cttcgacgac ttcgggctta
     1201 cccaaaagat acttatcttg attcttttca tcgaatcccg tggattccga aatccttgcc
     1261 gcattgtggg aaagctgttc ggggcgggag atcgctttgg aagcatcgta accgaagccc
     1321 aaagtcagat gatgtttcgt ccatttgttt ttcagcgatt tctcaaacga ggcattcaaa
     1381 acattgtgct gttcgcggta gtggaaacgg tcgctgctgt cgtaggaata cggcttgtcc
     1441 gccgacgcgc ggcaggattt gtccacagca ggatacacgg cacaattcag cttcagcgtg
     1501 ttgttgtcgg ttgccacgcc ctgtttgtca aacgacaaca ccgccttgtc cgcccaattg
     1561 tcagaatacg cttcgttttc ataacggtac agcaaaccca tacggcggcg gcggtgatgt
     1621 tcatcaataa atttggtgcg ggaatatttc aaacctatgc ccctgaccaa ctttttatcg
     1681 cccttccact cttctatatt cggcacaaaa tacaagccgt cgcggaaatc gtcgccgtcg
     1741 tacaccccgc tcttgtctct aaacttttcc gcctcgtccg taccgtaata ctgtttttcc
     1801 gtcatatcgc ggatgtcgta acgctgtttg gtatcctcaa acacaccgcc gacataatgc
     1861 ctgccgccga agcggtagcc cagcttggca agccacgaac cgctgcggta atccatcgga
     1921 tcgggcaata tcctgccgcc gcccgtgtaa gcttgggcgg acagattttc gtggcgcacc
     1981 tgcgcctccc gcacctgcgc ctcttcttca gcacttaaag gctgattttg ttcaatacgt
     2041 tcttttaccc agcggttgag ctggttgttc aaatatttcc cgtagcccgc caattttgcc
     2101 acgggcttgg attcacgctc gccctctact gagaaaaatt gccctgtttt atcttggctt
     2161 tgaatatcgt atgtctggcg gaacgcgtcc aaacggttta tgccgtatgc caccccgtcc
     2221 gcaatatcgc cgtgcgggcg cgtttcccgc ccctgacgtt cggttcggat tagcagccct
     2281 tcccaaccgt ctttgctgaa ccccgcgccg agcgacttca taaattggcg gtttttactg
     2341 ccgtaggcag tttttgcctg tatcccccag cttttgccgt ctgaaatcag gtctgccgcc
     2401 tctttggtgc ggaaggcgac tgcgccgccg agtgcgccgc tgccgtgatc ggacgaaccg
     2461 gcacctttgt cgatttccac cgtgctgatg ttttcatatt cgatttcgtt gattgcaccg
     2521 ctgccgccgc gtccgccgta tccgctcaac gatccctgca cggtaaacgc ctgtatttgg
     2581 gcaacgccgt caaccgaaac cgccacacgg tttttatcca cgccgcgtat cgagtagccg
     2641 ccgctcgcgc cgttgccctg ttcgacaacc gccacgcccg gatcgtagcg cgtcaggtcg
     2701 cggataccga gtacctgttc tttgttcaac gtttccgacg ttttgacgat tttgcccaaa
     2761 ccggtcgcct ctttcgatcg ccgtcccact ttggcggcac ggacggtaac ctctttcaag
     2821 gattgggtct gcgccgcatc aggtgccgcc ccccccgctt gggcagcata agccggaaaa
     2881 gcggttgcaa cagccaaggc agtcagggta agctgaaaac cgtgtttctt attcattttt
     2941 ccacctcttt attatctttc ttcgcaccga ataccgcgcc gaatttccgg tcgctgtcga
     3001 taatattacc gcccaattcc gccgcctgcg gtccataaaa gccgcctgtt acaagaagat
     3061 tgttggcttt gaagctctgc gggctggtcg aaccctgccc agaaaggtca ataccgttat
     3121 cccgagtgcg tgctgtcgcg tggaaaccgt tgccatcaat cacaccgttt tcaatataaa
     3181 cagcggggtg tgtatcattt ttttctgtca acttacctga aagcgacttc gcgccgaaat
     3241 caacgtcaaa ttctgctttt gccccttgat tcgcatagct atccttttga atgaacgtag
     3301 tgccaggctg atcgtctttc ttatctggta cgccgatacg cgcttcccaa gtgccggtat
     3361 agtgtgcttt tccagttttt ggaatgtcgg cttccgccgt gcggatacct ttcaggaaaa
     3421 ggtcgatgtc cctgccttta ggggcttccg gagcgggcag gatggcgttt gaaccgctgc
     3481 cgctttcttc tgtcggcgat tcttcttcgg tttcttcggc ttcatcagct tcagcttctt
     3541 cctcttcaac ttcatcagct tcttcttcga tttcttctgc atcttcttcg gaaacttcgt
     3601 cttcatcgtc gatttcgtct tcgccttctt cgacgctatc aacgcctgta tcctcttcgt
     3661 ccccctcttc atcctgcgcc ttcggttgga cggcggggcg ttcggttttt atccgtccga
     3721 gtttcacata ggtcagaaaa tcgcaacaag cacggacggt cattttcctg ccgtcggcaa
     3781 gctcgatgat tttctcttgg ctaaccaaag gaatttcacg cccttcgaca agaagtttgt
     3841 cgggatgacc aaaatcgggc atagtggaaa tggcaaactt acgggcatgg tcatcagtcg
     3901 cctcgtcaac ggaaattttc agagaatcca agattttggt gtgttttcca gacggcaggg
     3961 caggatttgt atctgatgcg tttgatgcgt ctgttttttg tttgcctgcg aatacgccga
     4021 atacgctgtt gtcgttgctg ataaaccgtc cggcaagctc ttctccgtta tcgccgaaaa
     4081 aaccgccctc aagccgctga tcggcatcgg catggaaaaa caaacgctca ttaccggcag
     4141 ggtttttcgc taaatcagga ttgaccttgg cactgccggt aaagcggttg ccgtccaatt
     4201 ttgcagtaat gtcgtaaatg gtcagcggtt tcttttcatc ggttttcctt tgcacatact
     4261 gatttttaat cagcttgcca ttcagagttt tttggtcgaa atcaaccgta tatttggcag
     4321 gatgcttttc cctttcgtcg acatccttag ccgcataaga agttgcacca acatcgttac
     4381 cataaaatgt gatataaccc aaattgtcaa tgccaaccgc cttacctgct cgatgacgtt
     4441 tgacatcggt catatattgc cagttaccag aatattccac cgttcccgca ctcggtaaag
     4501 attgggaagg acgttctcca gaataatata caaaaccatc ataacctaag cggtgggtaa
     4561 cctgcttacc acctgagttc tgttcgattt catctgttcc aaatctagta taaacatatc
     4621 cggcccggac atgttcatat ttatacacag catcttttaa atcggacgtg tagattttta
     4681 cattaggata ccgtccgttg atttcctttt taagtttgtc agcctgttct ttcggcgtac
     4741 cgtctaaaaa caggatatcc tcctctttga gcggcagata ctcctctgcc tgcttactat
     4801 tgggaatttc attaccattt ttatcaaaag ctgcgatatt ccgccttggc agccgcattg
     4861 ccgcaccgac ggcgggttga ttgaccggcg tggtttctac cgaaggcccg gcagggggcg
     4921 aagtgggaac gtccttagac ttgaaagtgg cggggtacgc ggtcggcgtt gattcgacaa
     4981 caggctgtac gccgaaattg ccgccgatac aagatgccaa aagtaagggc aacaagacaa
     5041 tgccgccata attcggttta cacatcccta cttttcctcc tatttgatta ataataatta
     5101 tcattatatt aatatgtaca gataatatca acccgttttt atagtgaatt aacaaaaatc
     5161 aggacaaggc gacgagccgc agacagtaca gatacattcc gtcattccca cgaacctaca
     5221 tc
//
LOCUS       DAWFAN010000139         5139 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWFAN010000139 DAWFAN010000000
VERSION     DAWFAN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.139"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            120..1016
                     /gene="glyQ"
                     /locus_tag="WGO65_001651"
     CDS             120..1016
                     /gene="glyQ"
                     /locus_tag="WGO65_001651"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0327926.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAVEEASLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLIKK"
     gene            1133..3196
                     /locus_tag="WGO65_001652"
     CDS             1133..3196
                     /locus_tag="WGO65_001652"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ0327927.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINVAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAAQMREQGKVVA
                     SFAERKAAIQTALEGQARRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLTNVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3213..4259
                     /locus_tag="WGO65_001653"
     CDS             3213..4259
                     /locus_tag="WGO65_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0327928.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIARRFQEQDGRIRILAQPRNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRPEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQTKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILRRLLKNR"
     gene            4301..5128
                     /locus_tag="WGO65_001654"
     CDS             4301..5128
                     /locus_tag="WGO65_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0327929.1"
                     /translation="MQNHVISLASAAERRAHIAETFGSRGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGEGAEQFLA
                     EDAWLEERFDKDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLQHFVMLQPEQIKPIDLMMFSDFFDKEGVLVYQVSPALCAQELHYTKFHNKS
                     SVLGSLLEDERSKIPKQKHKDRTLKERLIHALTKISREKKKWEERKKMSEAVIIPFR"
BASE COUNT         1329 a         1418 c         1329 g         1063 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacatt ctgggcagac aaaggctgca
      181 ccgtcatcca acctttcgac atggaagtcg gcgcaggtac ttcccacccc gccacctgcc
      241 tgcgcgcact cggccccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggccg ctacggcgac aaccccaacc gcctgcaaca ctattaccaa ttccaagtcg
      361 cccttaaacc cgcccccgcc aatatccaag acctttatct cgactccctg cgcgaattgg
      421 gcatcgatcc caaagtccac gacatccgct ttgtcgaaga cgactgggaa aaccccaccc
      481 tcggtgcatg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcattgact gcacgcctgt actcggcgaa attacctacg
      601 gcatcgaacg cttggcgatg tacctgcaag gcgtagaaaa cgtctatgac ctcgtttggg
      661 caaaaacgct ggacgggaat acagtaactt acggcgacgt gtaccaccaa aacgaagtcg
      721 agcaatccac ctacaacttc gaatacagcg atgccgactg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgctcg ccgttgaaga agcaagcctt gcgctgcccg
      841 cctacgagct ggttctcaaa gcagggcata cgttcaacct tttagacgca cgcggcgcga
      901 tttccgtaac cgaacgcgcc acctacatcg ggcgcatccg cgcgttgagc cgcgccgtgg
      961 cgcaaaaata tgtcgaaagc cgcgaaaaac tgggcttccc gttgattaaa aaataacgat
     1021 acgtcgtctg aaacaggttt tcagacggcc ttttttgcgt atccattaat acaggccgta
     1081 cggtatatgc aggctttata agcagggcct acggacaaac agaaagtctc atatgacaac
     1141 ccaaaccctt ttaatcgaac tccttaccga agaacttccg cccaaagccc ttaataatct
     1201 gggcaaccat tttgccgccg ccgttgccga aggcttggaa aaagcgcaac tggttgacgg
     1261 cgcagccgaa tttacggctt atgcctcgcc gcgccgtttg gcggttcaag tcaaaaacgt
     1321 caaagccgtt caagccgatc agaaaatcgt gaaaaaaggc cctgccgtgg cgaatgccgt
     1381 aaaagacggt gcgccgacca aggctttgga aggttttgca cgcggtgctg gagcgaaaat
     1441 tgaagacttg accatcgtcc acgacggcaa gcaggacgtg tatgcctacg aatacgtcca
     1501 aaccggcaaa ccgctgggcg agcttttgga agacattatc aatgtcgcag tgaaaaaact
     1561 gccgattccg aaagtgatgc gttggggcag tagcacgttt acctttgtgc gccccgttca
     1621 cgggctgatt gtgctgcacg gcggcgacat cgtaaacgtc agcgttttgg gtctgcaaag
     1681 cagcaatcaa accttgggac accgcttctt gtccaacggc gaaatcacca ttgaaaacgc
     1741 cgacagctac gccgcacaaa tgcgcgagca aggcaaagtc gtcgcttcgt ttgccgagcg
     1801 caaagccgcg attcagacgg cattggaagg gcaggcacgc cgtctgaacg cgaccgtcgc
     1861 cgccgatgag gccctgttgg acgaagtaac tgcattggtc gaatggcctg tggtattgga
     1921 agccggtttt gaggaacact tcctcgccgt gccgcaagaa tgcctgattc tgacgatgca
     1981 gcaaaaccaa aaatacttcc cactgctcga tcaaaacggc aagctgatga accgcttcct
     2041 gctggtttcc aacctgcaaa ccgaagatcc gtcgcacatc atccaaggca acgaacgcgt
     2101 attgcgcgcg cgcctgtctg atgccgagtt cttctacaag caagaccaaa aagcgactct
     2161 ggaaagccgc ctgcccaaac tgacgaacgt ggtgtaccac aacaaaatcg gttcgcaagc
     2221 cgagcgtatc gaacgcttgc aaagcatcgc cgcccatatc gccaaagcat tgggcgcgga
     2281 tgccgccgca gccgaacgcg ccgcgcgttt ggcgaaagcc gacttggtaa ccgaaatggt
     2341 cggcgagttc cccgaactgc aaggcacgat gggcaaatac tatgcccgtt tggacggcga
     2401 aaccgaagaa atcgccgaag ccatcgagca acactatcaa ccgcgttttg ccggcgacaa
     2461 gctgcccgaa agcaaaattg ccgccgccgt ggcactggcg gacaagctgg aaaccctggt
     2521 cggcatttgg ggcatcggcc tgattccgac cggcgataaa gacccctacg ccctgcgccg
     2581 cgccgccttg ggtattttgc ggatgctgat gcagtacggt ttggacgtga acgaactgat
     2641 tcagacggca ttcgacagct tccccaaagg tttgctcaac gagaaaacgc cgtctgaaac
     2701 cgccgacttt atgcaggcgc gccttgccgt gttgctgcaa aacgactatc cgcaagacat
     2761 cgttgccgcc gtactcgcca aacagccgcg ccgtttggac gatttgaccg ccaaactgca
     2821 ggccgttgcc gcgttcaaac agctgcccga agccgccgcg ctcgccgctg ccaacaaacg
     2881 cgtgcaaaac ctgctgaaaa aagccgatgc cgagttgggc gcggttaacg aaagcctgtt
     2941 gcaacaggac gaagaaaaag ccctctttgc cgctgcgcaa ggcttgcagc cgaaaatcgc
     3001 cgccgccgtc gccgaaggca atttccaaac cgccttgtcc gaactggctt ccgtcaagcc
     3061 gcaagtcgat gccttctttg acggcgtgat ggtgatggcg gaagatgccg ccgtaaaaca
     3121 aaaccgtttg aacctgttga accgtttggc agaacaaatg aacgcggtag ccgacatcgc
     3181 gcttttgggc gagtaaccgt tgtacagtcc aaatgccgtc tgaagccttc agacggcatc
     3241 gtgcctatcg ggagaataaa ttgcagcctt tagtcagcgt attgatttgc gcctacaacg
     3301 tagaaaaata tttcgcccaa tcattagccg ccgtcgtgaa tcagacttgg cgcaacttgg
     3361 atattttgat tgtcgatgac ggctcgacag acggtacgct tgccattgcc cggcgtttcc
     3421 aagaacagga cggccgcatc cgtatcctcg cgcagccgcg caattccggc ctgattccct
     3481 ctttaaacat cgggctggac gaattggcaa agtcaggaat gggggaatat attgcacgca
     3541 ccgatgccga cgatattgcc gcccccgact ggattgagaa aatcgtgggc gagatggaaa
     3601 aagaccgcag catcatcgcg atgggcgcgt ggctggaagt tttgtcggaa gaaaaggacg
     3661 gcaaccggct ggcgcggcat cacaggcacg gcaaaatttg gaaaaagccg acccgacccg
     3721 aagatattgc cgactttttc cctttcggca accccataca caacaacacg atgattatga
     3781 ggcgcagcgt cattgacggc ggtttgcgtt acaacactga gcgggattgg gcggaagatt
     3841 accaattttg gtacgatgtc agcaaattgg gcaggctggc ttattatccc gaagccttgg
     3901 tcaaataccg ccttcacgcc aatcaggttt catccaaata cagcgtccgc caacacgaaa
     3961 tcgcgcaagg catccaaaaa accgccagaa acgatttttt gcagtctatg ggttttaaaa
     4021 ccaggtttga cagtcttgaa taccgccaaa caaaagcagt agcgtatgaa ttgctggaga
     4081 aacatttgcc ggaagaagat tttgaacgcg cccgccggtt tttgtaccaa tgtttcaaac
     4141 ggacggacac gctgcccgcc ggcgcgtggc tggattttgc ggcagacggc aggatgcggc
     4201 ggctgtttac cttgaggcaa tacttcggca ttttacgccg gttgttgaaa aaccgttgag
     4261 aaccgccgct ttatccaaca gacaaaaaac aggataaatt atgcaaaacc acgttatcag
     4321 cttagcttcc gccgcagaac gcagggcgca cattgccgaa accttcggca gtcgcggcat
     4381 cccgtttcag tttttcgacg cactgatgcc gtctgaaagg ctggagcagg cgatggcgga
     4441 actcgtcccc ggtttgtcgg cgcaccccta tttgagcgga gtggaaaaag cctgctttat
     4501 gagccacgcc gtattgtgga aacaggcatt ggacgaaggt ctgccctatg ttgccgtatt
     4561 tgaggacgac gttttactcg gcgaaggcgc ggagcagttc cttgccgaag acgcttggct
     4621 ggaagagcgt tttgacaagg attccgcctt tatcgtccgc ttggaaacga tgtttatgca
     4681 cgtcctgacc tcgccctccg gcgtggcgga ctattgcggg cgcgccttcc cgcttttgga
     4741 aagcgaacac tgggggacgg cgggctatat catttcccga aaagcgatgc ggtttttcct
     4801 gcaacatttc gtaatgttgc agccggaaca gataaaacct atcgacttaa tgatgttcag
     4861 tgattttttc gataaggagg gagtgcttgt ttatcaggtc agtccggcct tatgcgccca
     4921 ggaattgcat tacactaagt ttcataataa aagcagcgtg ttgggtagtt tattggaaga
     4981 tgagcgaagt aaaatcccaa aacaaaaaca taaagacagg acactgaaag agcgattgat
     5041 tcatgcattg acaaaaatca gcagggaaaa gaaaaaatgg gaggaaagaa agaaaatgag
     5101 tgaggcggtt atcataccgt tccgataaac atcccggtg
//
LOCUS       DAWFAN010000140         4871 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWFAN010000140 DAWFAN010000000
VERSION     DAWFAN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.140"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            66..326
                     /locus_tag="WGO65_001655"
     CDS             66..326
                     /locus_tag="WGO65_001655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327930.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            433..957
                     /gene="nspA"
                     /locus_tag="WGO65_001656"
     CDS             433..957
                     /gene="nspA"
                     /locus_tag="WGO65_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ0327931.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDASYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            1093..1482
                     /locus_tag="WGO65_001657"
     CDS             1093..1482
                     /locus_tag="WGO65_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0327932.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAIAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1560..2390
                     /locus_tag="WGO65_001658"
     CDS             1560..2390
                     /locus_tag="WGO65_001658"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ0327933.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2405..3862
                     /locus_tag="WGO65_001659"
     CDS             2405..3862
                     /locus_tag="WGO65_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ0327934.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3903..4292)
                     /locus_tag="WGO65_001660"
     CDS             complement(3903..4292)
                     /locus_tag="WGO65_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327935.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKHPEIYQRFQHFFPFVELPLL"
     gene            complement(4474..4797)
                     /locus_tag="WGO65_001661"
     CDS             complement(4474..4797)
                     /locus_tag="WGO65_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327936.1"
                     /translation="MNILASDRNYELNIEIFIDNIIHFQITDESYKVKFSEYLFENKE
                     KNDWDRNPAINYFFEIIDDSYMDWLKEESFDFFEKKYYKAYIFFFSDSVIEVISSTEP
                     VFYSK"
BASE COUNT         1206 a         1493 c         1031 g         1141 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaaaa ccgtatgcct gcacctgcaa
      181 caatccgaca gataccgctg ttttttccaa accgtttgca agtttcaccc atccgccgcg
      241 tgatgccgcc accaccattt aaaggcaacg cgcgggttaa cggctttgcc gtcggcaaag
      301 cagccggata ccgctacgta tcttgaagta ttaaaaatat tacgatgcaa aaagaaaatt
      361 taagtataat aaagcagaat tctttaacgg attcttaaca atttttctaa ctgaccataa
      421 aggaaccaaa atatgaaaaa agcacttgcc acactgattg ccctcgctct cccggccgcc
      481 gcactggcgg aaggcgcatc cggcttttac gtccaagccg atgccgcaca cgcaaaagcc
      541 tcaagctctt taggttctgc caaaggcttc agcccgcgca tctccgcagg ctaccgcatc
      601 aacgacctcc gcttcgccgt cgattacacg cgctacaaaa actataaagc cccatccacc
      661 gatttcaaac tttacagcat cggcgcgtcc gccatttacg acttcgacac ccaatcgccc
      721 gtcaaaccgt atctcggcgc gcgcttgagc ctcaaccgcg cctccgtcga cttgggcggc
      781 agcgacagct tcagccaaac ctccatcggc ctcggcgtat tgacgggcgt aagctatgcc
      841 gttaccccga atgtcgattt ggatgccagc taccgctaca actacatcgg caaagtcaac
      901 actgtcaaaa acgtccgttc cggcgaactg tccgtcggcg tgcgcgtcaa attctgatat
      961 gcgccttatt ctgcaaaccg ccgagccttc ggcggttttg ttttctgcca ccgcaactac
     1021 acaagccggc ggttttgtac gataatcccg aatgctgcgg cttctgccgc cctatttttt
     1081 gaggaatccg aaatgtccaa aaccatcatc cacaccgaca aagcccccgc cgccatcggc
     1141 gcgtacagcc aagccgtccg agcaggcgac accgtttaca tgagcggtca aatccccctc
     1201 gatcccgcca cgatgaccgt cgtcggcaac ggcgatttcc gcgccgaagc gcgccaagtg
     1261 ttccaaaacc tgcaagccat cgccgaagcg gcaggcggca cgctggccga catcgtcaaa
     1321 ctcaacgcct acctgaccga cttgggcaat ttcgccgtct tcaacgaagt gatggcggaa
     1381 tttattgccg agcccttccc cgcccgcgcc gccgtcggcg ttgcctcgct gcccaaaggc
     1441 gtgcaggtcg aagccgaagc cgtcctcgtt ttgaacgcat aattccacgt tcggactgca
     1501 tgatatttca aaaccatgcc gtccgaaccg caacactttc ccacttcaca acatctgcaa
     1561 tggcacatta cgcaatcggc gacatacaag gctgtttcga cgaactgacc gcgctgctcg
     1621 gcaaaatcgg tttcaaccac ggcacggaca ccctctggct gacgggcgac atcgtcaacc
     1681 gcggcccgaa atccctcgaa acgctgcaat tctgcatccg gcacgaaaac agtgtgcaaa
     1741 tcgtcctcgg caaccacgac ctgcacctgc tcgctgtcgg ctgcggcgaa ggtgcgccca
     1801 aacgcagcga cacaatcgaa cccatactca aacaccccga cggaaaaaaa atgctcgact
     1861 ggctgcgtgc gcaaccgctg ttgatacgcg agggcagccg cgtgatggta cacgccggca
     1921 tcctgccgca atggcgcata accaaagccg aatcgctcgc tagagaagcc gaagccgaac
     1981 tgcgcggcaa aaaatacgtc aaattcttct ccaaaatgta cggcaacaaa ccggccgcgt
     2041 gggacgaagg tttgaaaggc tatgcccgcc tgcgcttcat cgtcaacgcc ttcacgcgga
     2101 tgcgcgccct gacctttaaa aacgaactgg atttcgacta caaatccaca gtgaaaaaaa
     2161 tgccgcctta cctgcgcccg tggttcaaag cccccgaccg gcaaaacctc gaccgcacca
     2221 tcatcttcgg acactggtcc tcgctgggct acacgaatgc cgacaacgtc atctcgctgg
     2281 acaccggcgc gctgtggggc ggacagctga ccgccgtcaa cctcgaaacg gaagaaatta
     2341 cccaagtcca agccgccggc ggcatagact ggaaaagctt cgcaaaataa cggaccgccc
     2401 cactatgcac aaaatcctgc ccatcgccca cgtcctctcc cgactgggta tgctgttttc
     2461 ctttatcctg ctgatacccg ccgccctctc ctacgccttt tcggacggcg cgtacaccgc
     2521 cttcgccacc accgcgaccg ttaccctttc cggctcgtgc atcgtccggc tcgccaccct
     2581 ccggttcagg cgcgaactgc gcccgcgcga cggcttcacc ctcgtcctga tgttgtggct
     2641 ggcgtttgcc gctatggcgg cgatgccgat gtacctgtat ttcccgaata tgggctttac
     2701 cgacgcattt tttgaatcga tgtcgggact gaccaccacc ggcgcgaccg tcatccccca
     2761 cgtcgacggg ctcgccccct ccgtcaactt ttggcggcat atgctcaact ggctgggcgg
     2821 gatgggcatc atcgtccttg ccgtcgccat cctgcctatg ctcggcgtag gcggcacgca
     2881 gttattcaaa gccgaaatcc ccggcattga caaagaaagc aaaatgtcgc cgcgcatttc
     2941 ccaagtggcg aaaaaactct ggttcggcta caccctgatc accatcctcg cggcagcctg
     3001 cctgcatttt gccgggatgg gctggttcga tgccgtctgt cacgcgatgg cgaccctctc
     3061 gctgggcggg ttttccaccc acgatgccag catagcttat tacaactccc ccctcatcga
     3121 agcggtcatc atcgttttca ccattgtcgg cggcatcaat tttgccaacc atttcgccgc
     3181 cctcaacagc cgcagcctca aaacctattg gaaagacgaa gaatgccgga cgatgctgct
     3241 gctgctctcc ggcagcatcc ttgccgccgc cctgtacctg tggcacaccg gctattacgc
     3301 cggttttacc gaatccctgc gctacaccgc cttcaacttc gtctccatcg gactggcaaa
     3361 cgggctggcc aacaccgact tcgcacaatg gccgctcctg atttccctgt ggatgttttt
     3421 cctcgccaac atcctcgcca actccggctc gaccggcggc ggcatcaaaa ccatacgcgc
     3481 cctcgtcctg ttcaaattca gcctgcgcga aatgatggtg ctgctgcacc ccaaagccgt
     3541 ccgcaccgtc aaaatcagcg gcaaggccat acccgaccgc ctcgcgctga ccgttatgtc
     3601 cttcatcttc atctacttta tgaccgtcgt cctcttcagc ttcctgctga tggcgagcgg
     3661 tatggaattt accaccgcct tcaccgccgt catcgcctgc atcaccaacg ccggccccgg
     3721 cttgggcgaa gtcgggcccg ccggcaatta cgccggtttg gacgtgatgc agaaatggat
     3781 ctgcgttacc gccatgctct tgggcagact ggaaatcttc accgtcttca tcctcttcac
     3841 tcccgcctac tggaagaaat aaaaaatgcc gtctgaaaac ttttcagacg gcatcttgtc
     3901 ctttaaagaa gcggaagctc tacaaaagga aagaaatgct gaaacctctg ataaatttca
     3961 ggatgtttct ctaaggcttt taatgctttt tcttttgaaa taggcggatc atagacatct
     4021 atccccctta agaaggcaat gcccgttaat gcattttttt gatcatttga aatgtaacca
     4081 taattaacga ctccaattac atcataaatt tcccctatac tcttatcaaa atcaatcaaa
     4141 tcaaaaatat acaatggaat tgtatcaata ggagtatctc tgacaactat atccaaccat
     4201 aatttaaatt cagaaagatt aatagcatca tagaacatac agactattgc aaatcctaaa
     4261 tcttcacaat tttctttact aattttccac ataattattt acctttatcc attgcttccc
     4321 aaccgataca tccggtattt tagcttctcc ttttccgtca aacactcctt tggttacctg
     4381 ctttgtgtca aaaataccgt ctgaaagccg aatatcgttt cagacggtat tttgattgtt
     4441 gggtttttaa tcagagcttt gtttgataat ttgttatttt gaataaaata caggttctgt
     4501 cgagctgata acttctatta cagaatcgct aaaaaagaaa atataagcct tataatattt
     4561 cttttcaaaa aaatcaaaac tttcttcttt caaccagtcc atataactat catctattat
     4621 ctcgaaaaaa taatttatag caggatttct atcccaatca tttttttctt tattttcaaa
     4681 taaatattct gaaaatttta ctttataaga ttcatccgtt atttgaaaat gaattatatt
     4741 gtcaataaat atttcaatat ttaattcata atttcggtcg gatgccaaaa tgttcacatc
     4801 ttgttcggta acacaaattt tgtccacata aaatctgtga ttattgttag aaaattgtaa
     4861 tggtttaatc t
//
LOCUS       DAWFAN010000141         4863 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWFAN010000141 DAWFAN010000000
VERSION     DAWFAN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.141"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(210..938)
                     /gene="rmpM"
                     /locus_tag="WGO65_001662"
     CDS             complement(210..938)
                     /gene="rmpM"
                     /locus_tag="WGO65_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ0327937.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1302..2252
                     /locus_tag="WGO65_001663"
     CDS             1302..2252
                     /locus_tag="WGO65_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ0327938.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2365..3099)
                     /gene="fnr"
                     /locus_tag="WGO65_001664"
     CDS             complement(2365..3099)
                     /gene="fnr"
                     /locus_tag="WGO65_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ0327939.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3304..4725
                     /gene="hemN"
                     /locus_tag="WGO65_001665"
     CDS             3304..4725
                     /gene="hemN"
                     /locus_tag="WGO65_001665"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ0327940.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTASV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1240 a         1331 c         1178 g         1114 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tccgaacctg caccccgatt ctgacgatag
       61 ccgtttcaga aaaccaccca aaaaaacaaa ccccgcaaaa gcgggggttt gtttcacatc
      121 atcgatgccg accgacgttt cacgaatatt gcaacagtac ctggtacaaa atccactaac
      181 ctcatgcatc ggcaagatat tgcctagcct tagtgttggt gatgattgtg tgccggcaca
      241 acctgacggg ttacgatgct gcggattttc acatccacac ggcggtcagg ttcgatacat
      301 gcaatcagag cctcacgttt tttggcttta gagactttcg cacccagttt ggcaacttcg
      361 gcttcacaaa cttgagtcat ttgcgcttga gattcgccca agccgacagc agaaattcta
      421 gaaacaggta cgccgttgct gaccaggttg tttgccacta cgtatgcgcg gcgttcggac
      481 agggcctgat tgtatttgtc agaacccata aagtcggtat ggccttcgac gcggacagat
      541 tggatattgg tttgacccag gcgttgcgcc aatactttca ggttgtcttg agcttcggcg
      601 cgcaatgaat ccttatcgaa accgaacagg gttttggcag acagggaaat ggtttcatca
      661 acatattgcg gagcctgctc cacaacgacg acaggagcgg gtgcgggttc gggttctggc
      721 tcgggttcgg gggcagcaac cgcatcgccg cattctacgc gaccttggct tgctttatca
      781 aagtaggcgt ttttccagca ttcgccatag ttgttgcgta cgatttcgtt cgactggccg
      841 cttacggtgt aaccctgaac ggacgcctcg cccgcaacag cagtgccgga agcgagcaat
      901 gcaacgaata atgcgcttaa tttcagctgt ttggtcattt tattccctca ttaaatttgt
      961 acagcagata cagaagcaac tgctgaattg ccaacataca ggccgctgta gaaacatggc
     1021 ggcaaatcgg atatttcagt ctccactata aagaagcagg gggaggactg tcaatactca
     1081 acgcctgaaa acaaccgtaa aacagcctgt ttcctgatag tctatcatgc cgaaaaatca
     1141 attccgtgtc attactttag ggggattttt tccatatcgc acgattgccg ttgcacaaaa
     1201 acaacaaaca caacggcaaa gccccatacc gtaccggcaa gaaaatatga taaattataa
     1261 acaatgttca gccacccgac acagacccac accgacccgc catgaaatta caacaattga
     1321 aatacgcctt agaagtttac cagcacaacc tgaacgtttc cgaagcggcc gaagccttgt
     1381 ttacttcgca acccggcatc tccaaacaaa tcaaattgct ggaagaagaa atcggcattc
     1441 agatttttat ccgcagcggc aagcgcgtgg tttcggtctc gcagccgggc aaggtggttt
     1501 tggatattgc ggaacgtatt ttacgcgatg tacagaacat taaaaatatc ggcagcgagt
     1561 ttaccggaca ggacagcggt tcgctgacgg ttgccacgac gcatactcag gcacgctatg
     1621 ccctaccctt gattgttgcc gattttgtga aacgctatcc gaaagtcaac ctgaccatca
     1681 aacaggggag ccctgccgcc atcgcacgta tggttacctc aggcgaggcg gacttggcga
     1741 ttgttacgga acggatagac gaccatcccg aactgggaaa acttccctgc tatgactgga
     1801 ctcatgcggt tatcgtaccg aacgaccacc ccttgctcga atgcagaaac cccctccgca
     1861 ttgaagattt ggcgaggttt ccgctgatta cttatgaatt tgcattcaat gcgggcagca
     1921 gcatcgcgcg ggcattttcc aaagcccgtt tggaacagcc ggatgtcgca ttggcggcgg
     1981 cggatacgga cgtattgaag acttatgtgc gcttgggttt gggcgtggga ctgatggcga
     2041 aaatggcgta caacccggat acggacggcg atttgcagct tgtggatgcg gcacacctgt
     2101 tcgagccgtc gccgacgtgg attgctttgc gcagcgatac ttatttgcgc ggatatgcct
     2161 acgactttat ccaagcgttt gcgccgcacc tgacacgcga gaaggtggat aggattctgt
     2221 acacgcccat cagcgaggat ttttcgattt aggcggctgc cggttttcag acggcacttt
     2281 gcggcagata caacaaacag gacagatgtt ttcgtctgcc ctgtgtttat tgagaatgcc
     2341 gtctgaaatg ttcgtacggg ttaatcaaat ggcgtgcgaa caaccggata ccatcttctt
     2401 caacacctgc agattgagga tcttgatgtg tttgtgttcg acggaaatca atccttcctg
     2461 atgaaattta gataatgtgc ggctgacggt ttcaagtttc agcccgagat aactgccgat
     2521 ttcttcgcgg gacattctta agatgaagtc gttggcagca aaacctcggg aataaaggcg
     2581 ttgggaaagg ttcaacagaa aggcggcaat ccgctcttcg gcgcgcatat tgcccaacag
     2641 cagcataaca ccttggtcgc gcacgatttc acgactcatc atgcggaaaa agtgggtacg
     2701 caggctgggg atattctgcc ccagttcttc gatgtgggta aacggcagtt cgcacacttc
     2761 gctgtcttcc aaggcgaccg cgtcgcaact gtgcacatgg gaacagatgc cgtccatgcc
     2821 gatgagttcg cccgacataa agaaacccgt tacctgatcg cggccgtcct gactggcgac
     2881 ggttgttttg aagaagcccg aacggatggc aaagagcgag gtaaaggctt cgccgacaca
     2941 gaacaggtat tcgccctttt tcaggcggcg gctttgacgg atgacggcat cgagttggct
     3001 gagctcgttg ggcagcagcc cgacaggcag gcagagttcc cgcaaagaac aggaagaaca
     3061 cagcgttttc atctgatgtg tagtattatg cgaagccata ccgtaccttt ttgtgcgctt
     3121 tgccccatca tgattatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgtc
     3181 cttatttaaa tttaatccac tatatgtgct tattgacaca tatcaagaca ggtttatcat
     3241 actgtggcat tctaccaaac tatccaaaat ttgactaaca tcataaaacc gcacacacac
     3301 accatgaaaa tcattcagat acagaacaat cacaatgtaa acgatgaccg ccccgagttt
     3361 gaccgcgcgc tgattgccag cctgcccgcc agcggcccgc gctacacttc ctaccctacc
     3421 gccgaccgtt tccatgacgg tttccgcgaa ggcgaatata tcaaagcttt acatttgcgc
     3481 ggtatgggcg cgttaaacaa accgctttcc ctttacattc acattccgtt ctgcaacacc
     3541 atctgctact actgcggctg caacaaaatc atcaccaaag acaaaagccg cgccgatgcc
     3601 tacatcgaat atcttgaaaa agaaatggaa ctgctcgctc cacatctgaa cggacggcac
     3661 cagcttgccc aactgcactt cggcggcggc acgccgacct ttttgagcga cgaacagatc
     3721 gaacgtgtct tccgcatgat acgcaaacat ttcgagttaa tccccaccgg cgaatactcc
     3781 atcgaaatcg acccgcgcaa agtcagccgc gacaccgtcc tcatgctcgg cagactcggc
     3841 ttcaaccgca tgagcatcgg cattcaggat ttcgacccca aagtgcaggc agcggtcaac
     3901 cgcatccaaa gttacgaaga aaccaaagaa gtcatcgatg cggcgcgcga agcggggttc
     3961 aaatccgtca gcgtcgattt gatttacggc ctgccgcacc agacttcgga aagcatcaaa
     4021 accaccatcg ataccgtttt gtcgctcgat cccgaccgcc tcgcccttta tcactacgcc
     4081 cacctgccgc acgtgttcaa accgcaacgc cgcatcgata ccgcctccgt ccccgacagc
     4141 gaagagaagc tcgatatgct gcaatactgc gtccaaaccc taaccgaacg cggctacgtc
     4201 ttcatcggca tggatcattt cgccaaacct gacgacgaac tctccatcgc cctcaaagaa
     4261 ggcttcctcc agcgcaactt ccaaggctat tcgacctacg cggattgcga tttggtcgcc
     4321 atcggcgtgt cgtccatcgg caaaatcggc agcacctatt cccaaaacga acgcgacatc
     4381 gatgcctact atgccgccat cgacgaaggc agactgccca tcatgcgcgg ttatcagctc
     4441 aatcaggacg acatcctgcg ccgcaatatc attcaggatt tgatgtgccg cttcgcgctc
     4501 gactaccgga tttacgaaag tatgttcggc atcccgttcg accgctactt caaagacgaa
     4561 ctggcggatt tggaaaaact cgccggtttg ggattggtgc gcctgaacag ccacgggctg
     4621 accgttaccc cgaaaggacg cttcctcatc cgcaacatcg ccatggtgtt cgactaccac
     4681 ctgcgccaca aggaaaccaa ggcgaaatat tcgcaaacgg tgtgattgcg tctaacgtac
     4741 aaatgccgtc tgaaaggctt tttcagacgg cattttgctg ccggcaggat aagtgttttc
     4801 aagaacaggc ggcggcatat cataacgttc cgcacctttg tgtccgaccg ttccgaaacc
     4861 aag
//
LOCUS       DAWFAN010000142         4831 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWFAN010000142 DAWFAN010000000
VERSION     DAWFAN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.142"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(92..574)
                     /locus_tag="WGO65_001666"
     CDS             complement(92..574)
                     /locus_tag="WGO65_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0327941.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(727..888)
                     /locus_tag="WGO65_001667"
     CDS             complement(727..888)
                     /locus_tag="WGO65_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327942.1"
                     /translation="MSGQPEKHHTSPIEDKRKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(962..1423)
                     /gene="recX"
                     /locus_tag="WGO65_001668"
     CDS             complement(962..1423)
                     /gene="recX"
                     /locus_tag="WGO65_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ0327943.1"
                     /translation="MKPQKSLRARAMDILSRQELSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1491..2198)
                     /locus_tag="WGO65_001669"
     CDS             complement(1491..2198)
                     /locus_tag="WGO65_001669"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ0327944.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            2605..3870
                     /locus_tag="WGO65_001670"
     CDS             2605..3870
                     /locus_tag="WGO65_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ0327945.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEAIIKEFQTKGFITNE
                     AGYGKEISNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3928..4734)
                     /locus_tag="WGO65_001671"
     CDS             complement(3928..4734)
                     /locus_tag="WGO65_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ0327946.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1044 a         1102 c         1420 g         1265 t
ORIGIN      
        1 aatttaatcc actatatttg cttgatttca tccgtttgca atggaaacgg atggcgcgat
       61 gtgcctgtct ttcagacggc atagtccgcc gtcagcagcc gcaggacatg tcttcagagg
      121 cttgcaggct gatgtctctg cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg
      181 tcaggatttc cagcggaggg acagggacgg gtttgccgtc tttgaccgca accatcgtga
      241 agtagcagct gttggtatgg cggatttcgc cggtgcggat gttttgtgct tcgacacgga
      301 tgccgatttc catcgaggtg cgtcccgtgt agttgacagc ggcgtaaaag gtaaccaaat
      361 cgccgatgtg gatgggttct ttaaacagga ctttgtcaac cgacagggta acgcaataat
      421 tgccgctgta acggctggcg caggaatagg cgacttggtc gagcaggagc aggagttcgc
      481 cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg gacataatga
      541 gttcgtgcga aggcagttgg cgttgttgtg tcatagtgta cctgttttaa gaaagggtgt
      601 tttgaaatgg gtgcggggaa attttaggga atggggaagt ggaattcaag ctaaaaaaag
      661 ctatgatggg atagtctgcc gaaatggacc gccgatgccg tctgaagcct tcagacggca
      721 ttttgtttag gaaagccaaa aacggaagac caggaaaaac acaagtgcca atacggcgac
      781 ccaaacgacg agcatgaatc cggcaactgc ccgtcccata cggtaaaccg ggtttttgcg
      841 tttgtcttcg atgggggagg tgtggtgttt ttcgggttgt ccggacattt tgttctcaat
      901 cggaaaagtt ggaaacgggc gtaaggttat gttgccgtgc aaaaagattc aagaaggaag
      961 gtcagcagcc gtcttcccaa ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat
     1021 cggcatcgaa tccgcgatag gcgaggaaac gcgcctgttt ttgtttttct ttgaggtcgg
     1081 cggcggggtg tttgaatttt ttacgcaaca cggctatggc ggcctgtttt tcgcttgagc
     1141 ggtcgggaag caggttgcgg ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt
     1201 tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg gcatagcgca
     1261 aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac tcctcttcgc
     1321 tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg gctgagttct tggcgcgaga
     1381 ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg gtttcggcgt
     1441 ggggcaggtt tcagacggca tagcggcgca aaccggagcc gtccgaatgc ctactcttct
     1501 ttgtttttct gaggttgcag gttttcgtaa atttcgggca gcgagccgat aatccagtcg
     1561 gggcgggtcg catcgtcttg cgagagcagc gtcatatcgc cgtaaccgaa ggtaacgccg
     1621 acgctcaggc agccggcggc tttggcggcg atgatgtcgt tgcgcgagtc gccgaccata
     1681 accatgtttg caacatcgat acccaaaact tcggcggcgt gccgcagcgg cagggggctg
     1741 ggttttttct cgggcaggct gtcgccgccg agtatcaggc tgaagtagtc ggcaagtccg
     1801 agctgtttca acagttcgac ggcaaggatt tcgtttttgt tggtaatgac tgccagcggg
     1861 atgcccaaag atttgagcaa tgccagcccg gcttcggttt cgggataggg gcgggtgaag
     1921 acgctcaaat ggtcgcggta gtatttcata taggatacga aacctttttc ccacagttcg
     1981 gaatcggctt cgcggtcgcg gtcgttggtg aggacgcggt gaaccagttt gccgatgccg
     2041 tcgcccacat agctttcgac cactttggcg ggcagcggtt tcataccgag ttgttccaac
     2101 atcgcttctg cggcggcggc aaggtcgggg acggaatcgc acagtgtgcc gtccaaatcg
     2161 aaggcgacgg cttggacgtg ttcgatggct gcattcataa tctgatttcc tgtcgggttg
     2221 aaagggttta ttttaacatc gtttgcacag ggcttctaat gtatagattg ttaacaatcg
     2281 caacaggttt tgcaggcggt gttttcggat ttcggggcgg aatcgggcaa gggtttgaaa
     2341 tcccagcgat gttccaatta aatttttaaa aaataaaagg atgcctatat ctttatttaa
     2401 tttgacattt atttttcgga cgcgcgcgct gctttttcag gcgaagtccg ggctgattcc
     2461 gtgcggcttg aaaaataaaa tcatatatag attaatcaaa tcgggcttat gccgactaca
     2521 cgctattaca ttttgtcggg aaattggtta tagtaatgag cgaaatgcac gcccctgaaa
     2581 atctttttgt gaaaaggaag caaaatgtct gaagcccttg caaaagaaac cctcaatccg
     2641 tttgaaatcg cgcgcaaaca ggtcaaaacc gcctgcgacc ggctaaaaac cgatccggcc
     2701 gtttatgaaa tcctgaagag tccgacccgc gtgttggaag tcaattttcc cgtcaaactc
     2761 gacgacgaca cggtcaaaac cttcacgggc taccgttcgc aacacaacaa cgccgtcggc
     2821 ccctataaag gcggcgtgcg cttccatccc ggagtgaatc tggacgaagt caaagccctg
     2881 tcgatttgga tgaccatcaa atgctgcgta gcgggcattc cttacggcgg cggcaaaggc
     2941 ggcgttacct tggatccgcg cgattattcc gaggcggaac tggaacgcat cgcccgcgct
     3001 tattccgaag ccatttctcc gctgatcggc gagaaaatcg atattcccgc acctgatgtg
     3061 aacaccaacg gcaaaatcat gtcttggatg gttgatgcct atgaaaacgt ggtgaaacat
     3121 tctgcaccgg gcgtatttac cggtaaaccg gttgagttcg gcggctcttt ggcgcgtacc
     3181 gaggctaccg gttacggcgt gaacctcgcc gccgtccaag ccttggaaaa actgggcaaa
     3241 gacgtgaagg gtgccactta cgccatccaa ggcttcggta atgtcggtta ccacacaggt
     3301 tattacgcgc atcaatccgg cgcgaaagtt gttgcggttt ctactgttga cgttgccatt
     3361 tacaacgaaa acggcttgga tatggaagca atcatcaaag aattccaaac caaaggtttc
     3421 atcaccaatg aagccggcta cggtaaagaa atcagcaatg ccgaactttt ggctctggaa
     3481 gtggacgtac ttgccccttg cgcactggaa aaccaactga cttctgaaaa tgccggtaaa
     3541 gtccgcgcga caatcgtggt cgaaggcgcg aacggcccga ctacgcccga agccgacgtt
     3601 atcctgcgtc aaaacggcgt attggtcgtg cccgatattt tggcgaactg cggcggcgtg
     3661 gtggtatctt attttgaatg ggtacaaaat ctgcaaggct attattggga gtttgacgaa
     3721 gttcaggaaa aagaaaccgt cgtcctgcgc cgtgcgttcc gcgatatatg gaacttggcg
     3781 caagagtatg atgtcgattt gcgaaccgcg tcttacatga tgagtatccg ccgtgttgaa
     3841 aaagcgatga aactgcgcgg ttggtattga tgtttgaaca aaaaatgccg tctgaagcct
     3901 tcagacggca tttttgtgcg ggcgggatta atggtgcgaa tggccgattt ggaaggttaa
     3961 agtcgagtag ttcgcctgtt tttggcacac gctttgatcg gggaagtcgg ctttgtgttc
     4021 gacattggct ttccagaagc cttggcgcaa ggggatgatg tccacttcgc ctttgtcgtc
     4081 tgtgctgtcg gagaaagcct gtgcttcggt tttgtgcgtt ttgctgcggt cgctggtgtc
     4141 gaagccgtca aaggtggcgg taacggtggc attgggcagc ggttcgccac ggaacagaac
     4201 gcggactttg aagcgttcgc ctacgtgaat gttggcggga ttgtccagcg ggacgatttc
     4261 caagttttgt ccgaccggtt tggtgatgat ggcggtgtcc gcgctttcgt gtccgacgtt
     4321 gacgatgttt ttgccgaaca ttcgggtttg ttcgcaatag cttgcgtcag gcatttcttt
     4381 gatgcccgcc tgtttccagc ctgctttgtt ttttgaccag aaagtaggct gatattcggc
     4441 gatgacgagg taactgccgt ccttaacggg acggttgctt cggtactggt agttgtatgt
     4501 gccgcgttga atcatgtttt ccttgccttt ttcggtaacc agctgcatcg gtttgctgaa
     4561 gatgtgcagg cggtctttgg cgatgggttc gagttcggga aattcgccgt agcccaagtc
     4621 ggctttaagg tattcgccgc cgtgcgtgtg ggcggtttcg acccagacgc ggtgggcgtg
     4681 ggcggatgtg gcaaataggg cggaaacggc gagcagtgtc aatgttttct tcatggtgca
     4741 gcctttgtct cgtgtgggtc aaagattttg ttatgttata acaaaatagg tatcgtgtga
     4801 agacggattt gctgtctgaa aactgtcttt t
//
LOCUS       DAWFAN010000143         4717 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWFAN010000143 DAWFAN010000000
VERSION     DAWFAN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.143"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(416..952)
                     /locus_tag="WGO65_001672"
     CDS             complement(416..952)
                     /locus_tag="WGO65_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ0327947.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTAD
                     SVRLLYSFYTAVGFCNEKMYLFEAESVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(986..1342)
                     /gene="folB"
                     /locus_tag="WGO65_001673"
     CDS             complement(986..1342)
                     /gene="folB"
                     /locus_tag="WGO65_001673"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ0327948.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGIPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1398..2000
                     /gene="plsY"
                     /locus_tag="WGO65_001674"
     CDS             1398..2000
                     /gene="plsY"
                     /locus_tag="WGO65_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ0327949.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILLLLRHKSNILNLIKGKESKIGEKR"
     gene            2088..2933
                     /locus_tag="WGO65_001675"
     CDS             2088..2933
                     /locus_tag="WGO65_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ0327950.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYWRVTETLKSALKRFESGCYLIWYPCLSREESRKLPEELKKLVPD
                     NYLHAELHIHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3182..4156
                     /locus_tag="WGO65_001676"
     CDS             3182..4156
                     /locus_tag="WGO65_001676"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ0327951.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYILYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(4315..4533)
                     /locus_tag="WGO65_001677"
     CDS             complement(4315..4533)
                     /locus_tag="WGO65_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ0327952.1"
                     /translation="MFPEYRDLISRLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1212 a         1393 c         1001 g         1111 t
ORIGIN      
        1 ctcaaattca aattgggctt ttaggtctat aacaaagaaa aagccttggt aggataaacc
       61 taccaaggct ttttcttaaa aaaacgctct attaccgtgc ctaaaaatgc aaaaaagtaa
      121 ccgtcataac ccttttgcca aaatcaatat catcatccga tgaatgaatt gacggtatat
      181 aagaaagaat taataagctc ataaaccggt aagaaaaatc agtaacaaag atattcatta
      241 ttaaccattt taaaaattat catgttcgtt tgggtaatta ttataccctt ttccctctta
      301 ttactaaagc acgaatgact ttaaaggacg cgcccagaga caagccgaaa aggcatatct
      361 catccgccaa acaaaatgcc caccgactta ctccggcagg cagttcaaca ccctgtcaat
      421 ccttcatcag ccagtattgc aaaccgatta atgtcttgcc gtctttaatt tcatcgtttg
      481 ccaatgcctg acggacttct tctttcgaca tcaatacggt ttccgtaatc tcgtcttcgt
      541 cattggcaag cgtactgccc aaacgcacgc tttccgcttc gaacagatac attttttcgt
      601 tgcaaaaacc gaccgccgta taaaaactgt aaagcaggcg tacgctgtcg gcggtataag
      661 gcgtttcctc cgccaattct cgcagcgcac acgctgccat atcctcaccc gccacatcca
      721 gcttgcccgc aggaagttcc aatgtcgcct gatttgccgc ataacgccac tgccgcacca
      781 aaacgacctt ttcatcatcc gttaccgcca acacgcacgc cgcacccgga tggcggatga
      841 cgatacgttg cccttcattg ccgttgggca acctgacctt atccctgctg atgctgacga
      901 aaccgccctc gtaaatggtt tcgccgccta attttacttc cctcaaatcc acttcatatc
      961 tcctttccgt ttcctattat gccgttcaat cttcacgctt gccgcgctcg atttccacgc
     1021 caacctcgcg cacgccttct aaaatacccg gcttgacgat tttcacgcgt acccacaccg
     1081 cgccgaaata tcccaaaacc aaatcggcaa tatattccgc caacgcttcc aaaagcagga
     1141 aatcctgttc tttcagatgt cggcgcaatg tttcgcatac ctcggcataa tgcaccgtat
     1201 tggcaatatc gtcgtccgaa cccgctttct cgggaatgcc gatgtccaaa tcgacaatca
     1261 gggtctgcaa ccgttcgcgt tcccagccgt acacaccgat gagcgtatcc gccttcatgc
     1321 cgtgcaaaaa gattttatcc ataatccgcc ccgttttttg ctaaaataac cgcaccattc
     1381 taagtaaagt accgcaaatg ttcaacatac cggctgttgc cgtttcctat ctgatcggct
     1441 cactttcttt cgccgtcatc gtttccaagt attacgggat ggacgacccg cgcacctatg
     1501 gttcgggcaa tccgggggca accaatgttt tacgcagcgg caaaaaaaag gcggccgcgc
     1561 tgacgctctt gggcgatgcc gccaaaggtt tggttgccgt tttgcttgca cgcgtgcttc
     1621 aagaaccgct cggtttatcc gacagcgcaa tcgcggccgt cgcactcgcc gcgctggtcg
     1681 ggcatatgtg gccggtgttt ttcggattta agggcggcaa aggcgtggca acggcattgg
     1741 gcgtgcttct ggcactctct cctgcaactg ccttggtctg cgcgttgatt tggcttgtga
     1801 tggcattcgg cttcaaggtg tcctcccttg ccgcattaac cgccacaatc gccgcccccc
     1861 ttgccgcact gttttttatg ccgcatactt cttggatttt cgcaaccctc gcaatcgcca
     1921 tattgctgtt gctccgccat aagagcaaca tcctcaacct gattaaaggc aaagaaagca
     1981 aaatcggcga aaaacgctga ttcggcattt gttttccatt acgtcatgcc gtctgaaacc
     2041 atgttcccgc gttcagacag catttccgca caaacggctt tcataccatg ctcagttaca
     2101 gacacgcatt ccacgccggc aaccacgccg atatgctcaa acactttacc ttgtttttag
     2161 tgctgcaata tttcaaccgc aaagacaagc cctactggta catcgacacg cacggcggcg
     2221 cgggtgtgta caatctcgaa ggcagcgagg cgcagaaagt cggcgaatac cggcaaggca
     2281 tcgccctgct ccgacaggcg caaaacctgc ctgccgaact ctctgacttt gccgcgcaca
     2341 tacaaaaaat cctgccctcg cccgaacttt actgcggttc tccgtggctg gcacaatcgc
     2401 tgacccgcgt cggcgacaaa ttacgcctgt ttgagctgca ccccaccgac tttgtccatc
     2461 tgcaaaacaa tatgggcgaa gcaggattgg gcaaacgcgg acaagtgttg cgcgaagacg
     2521 gttacaaagg gctgatttcc ctgctgccgc ccccgccgcg ccgtgccgcc gtcctcatcg
     2581 acccgcctta cgaggaaaaa caggactact ggcgtgtaac ggaaacgctg aaatccgctt
     2641 taaaacggtt tgagtccggc tgttatctca tctggtatcc ctgcctaagc cgcgaagaaa
     2701 gccgcaagct gcccgaagaa ctgaaaaaac tcgttcccga caactacctt cacgccgaac
     2761 tgcacatcca cgcgccgaaa gccgacggtt tcggtatgca cggcagcgga atgttcgtca
     2821 tcaatccgcc ctatctgttg gcggaacaat tggctgcaaa cctccccgcc ctgacgcggc
     2881 tgctggcgca agacgaaggc gcgcgctacc tgttggacag caaaatccgc tgaccccgat
     2941 gccgtctgaa acaacccttc agacggcatc tcccgtcaaa caaacaaaac cggacgctat
     3001 accggcagtc ggcactttta agaataaata tcccctcatc cgcctgccgc gttccgactt
     3061 gccccatgcc gtttttttac actacaatcc cctagactat cctgcaaaac gcaccaaagg
     3121 aatacgccac aataacctgc cgcctttcag acggcacaaa cctttccgaa acgagcaaac
     3181 tatggacaca ctgacccgat tcctccccga acacctgcaa caaaaccagc tgcccgaagc
     3241 actcggcggc gttttactgt ccgttgtttc cgcctgcacc gaaatcaacg ccaaagtccg
     3301 cctcggcgca ctggccggcg tattgggtat ggcgggcacg ggcaatattc agggcgaaga
     3361 ccaaaaaaaa ctggatgtta tcgccaacaa catcatgatt gacacactca aagccaaccc
     3421 tgccgttgcc ggtttggcaa gcgaggaaga agactctttc gtaagtgccg gtgaaaacgg
     3481 acgctatctc gtcctattcg accctttaga cggatcgtcc aatattgatg tcaacatttc
     3541 cgtcggtaca attttctcca tccttgaaaa acccgaaggc gcattggcaa ccgaatcatt
     3601 ccttcaaacg ggcagacagc agcttgccgc aggctatatc ctctacgggc ctcaaaccca
     3661 gctcgtattc acattcggac acggcgtata tatgttcacg ctcaatgccg aaaacgaatt
     3721 tgtgctgacc aaagaaaaac cgaaagtacc cgaaagtacc aaagaatttg ccatcaatat
     3781 gtccaaccgc cgccactggc tgccccccgt ccaacaatac atcgacgagc tcttggcggg
     3841 cgaaaccggc acgcgcggca aaaactacaa tatgcgctgg gtagccagca tggttgccga
     3901 aatccaccgc atactgatgc gcgggggcgt gttcatgtat ccgcaagaca aacgcgaccc
     3961 gtccaaaccc ggcaaactgc gcctgatgta tgaagccaat cctatggccc tgatactcga
     4021 acaagccggc gcatcggcaa gcaacgcccg tcaggacata ctcggcatcc gtcccgaaag
     4081 cctgcaccag cgcgtcgccg tcattatggg cagcagcgaa gaagtggatt acctcaaccg
     4141 cctgcattca aaataaaagg cagcctgacc gataaagaaa ggcggcagcg cgcatccgac
     4201 gctgccgccg atatttcgtt tatagcctta aaaagataga aaaccacctt tccgaacggc
     4261 aaaaatgccg tctgaaaagt tttcagacgg cattttaaaa attaaagccc gaatttattt
     4321 tcccgctgct ttttgcagga tggcgtacaa ctcgtctttc agtttcaatt tggctttttt
     4381 cagctcatcg atggtttccg caccgctggt aaccggattg ttgaccagac cggtaatttt
     4441 atcgtccagc tcgttgtgtt cgtcgaacag acgggcgaag cgggaatttt cctgtttcaa
     4501 tctggaaatc aaatcacggt attctgggaa catatcgctt tcctctcttg gtttacggtt
     4561 aaaaagtcaa actgaatctt tacattaatt ataacaaaga taatattttt atccagtgtt
     4621 ttatcggata cggcaaaatt ttcttttaac accgccgtcc ggttgataaa aacgttgaca
     4681 atatcacgct ttacgcataa attttcagcc tttccta
//
LOCUS       DAWFAN010000144         4643 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWFAN010000144 DAWFAN010000000
VERSION     DAWFAN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.144"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(200..1138)
                     /locus_tag="WGO65_001678"
     CDS             complement(200..1138)
                     /locus_tag="WGO65_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0327953.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1147..2022)
                     /locus_tag="WGO65_001679"
     CDS             complement(1147..2022)
                     /locus_tag="WGO65_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0327954.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2204..2280)
                     /locus_tag="WGO65_001680"
     tRNA            complement(2204..2280)
                     /locus_tag="WGO65_001680"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2244..2246),aa:Met,seq:cat)
     regulatory      2430..2529
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2620..4521
                     /gene="thiC"
                     /locus_tag="WGO65_001681"
     CDS             2620..4521
                     /gene="thiC"
                     /locus_tag="WGO65_001681"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ0327955.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTADRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFTELHTLGELTSKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1127 a         1314 c         1229 g          973 t
ORIGIN      
        1 gaatgacgat gaaaagattt ttgttgtttc ggataaattt ttgccgtgtt gggttctaga
       61 ttcccgcttt tgcgggaatg acggcagggt ggtttctgtt gtttcggata aattttgcag
      121 ccctgataaa aaatatggct gctttggtaa aaaatgccgt ctgaaaggtt ttcagacggc
      181 attttgtttt taagaagcat cagcggaagc ggacgatttc cctgtcttcg atatggatgc
      241 gtaccgtatc gtttcccgat atttcaccgg cgtgcggcat atcgaggttc agccacagga
      301 tgccgtgttc cggatggagg acggacaggc tgaacgattc gggcaaacag gtacgggata
      361 atacgcggca ctccatgccg tcttggtcga aacgcaccgc atgttgcgga atatggcggt
      421 catcgtcggt attgggcaaa cccatcagtc gggcgacctg cacgccggca ggcgtttgaa
      481 ccaaggtttc gggcgtaccg cattgaagga ttttcccctc gtgcatgacg gcgatttcgt
      541 ccgccgccgt gcaggcctct tcgggcgaat gcgttaccaa aacggcaggg atgccgccct
      601 tgcggatgcg ttcggcagtc atgcggcgca gcgtgccgcg caaatgcgtg tccaaactgg
      661 aaaacgattc gtccaacagc agcagggaag ggcggacaac caaagcgcgc gccagtgcca
      721 accgttgttt ctcgcctccg gaaagttttt caggcttgcg gtgcacctcg ttttccagtc
      781 cgacttcggc aagtgccgcc atggcaaggc gttcggcttc ggctttcggc attttttgca
      841 ttttcaaacc gaatgccgca ttttccagcg cactcatatg gggaaacagc gcgtaatctt
      901 gaaacatcag cgaaatacgg cgtttttcgg gcggcatacg ggtaatgttt tccccgttca
      961 gctgaatttc gccgccgtcc ggccggacga tgcccgcaat catattcagc agggtggatt
     1021 ttccgcagcc cgaccgcccc agtacggcga gtattttgcc gcgcccgaca gtcaggcaga
     1081 tgttgtcggc aaccgttttg ccgccgaagc gtttgcagag tccgttcagt tcaagcatgg
     1141 cgcatcctat aaacgtatgc cgtccagcca ctcggacagc ggatgggcgg catcaatcag
     1201 accgtaacgg caaaacgcgt ccagcgtaac cagttgcgcg tcgtgcatca tgtttcccga
     1261 caacatggtg gcaaccaagc tgccgatgtc cattttttca aaacacgcaa cctcgccgtc
     1321 ctgattttca ggcaggaagg tttcgggtag gacggcatcg aatacataca ggatttcatt
     1381 gtgcacaccc cggctgacgg ggcgcaggct gtgcagccga gataccggac ggataaacgg
     1441 aaacagcgtt ttatccaaac cggcttcttc gctgctttcg cggcacacgg cttcagacgg
     1501 catttcgccg ccggaaacac cgccggcggc aatattgtcg agtttgtcgg gatcgactgc
     1561 tttgtgcgga ctgcgcttgc ctatccagaa atgccatcgg ccgtccgatt cggtcagtcc
     1621 gttgagatgg acggcgcggc tgagcagtcc gaacggacgg aaagcggcgc gttcgagcgt
     1681 gaacaagggg ttgccgccgc cgtcggtcag gtcgaaacac tcgttgcgcc atccgtgaag
     1741 cagccccgcc ttgttccatg tgcgggcgag gtgctgcaag cggccgccca tatctgacca
     1801 gccgtccgca ttcaggaaaa tgccgtctga agactccgag cagcctgcct cccagtcttt
     1861 tttgacgcgc tccgcccatt ccggcgacag attgcccaaa ggcagacggt tcagatacag
     1921 cgttttccag caactttctg caccgtaact tgcttttgcc cactcgaaca gggcatcaag
     1981 gtcgtgtttg ctgacggatt cggtaaaacg gacggtcggc atagtcggct ttcttgaaga
     2041 caatgcggaa gattgtagcc aaactgcctg aatttgcaac cgcaaacaga cggcgggctg
     2101 ccgttccgat attgcggcag gaaggaaaat ccggcaaaaa acggaggcgg caggaaacag
     2161 gcaaaacaaa acccctcggc attgaaccga ggggtttgat atttggttgc gggagcagga
     2221 ttcgaaccta cgaccttcgg gttatgagcc cgacgagcta ccatgctgct ccatcccgcg
     2281 tcagaagatg aaactatacg gcagattttt tatgttgtca aacattattt ccgcgaaaaa
     2341 tcataaaatt ttgccgtccg gtggtttatg cctgatttga aatattattt tctttacaaa
     2401 agttcatgtt cgtgatttaa ttttggttaa cattgaaaca ggggtgctgc atgatgttca
     2461 ggcggctgag aaataccctt tacacccgat ctggataata cctgcgtggg gagttttcac
     2521 ggattctgct tttcagacgg cattggtttt caaatgccgt ctgaaaacgc aaaacgctcc
     2581 tgtttcttta ttctaaacga gaaaacagga gcatttttta tgactacgcc aaaaaaaacc
     2641 gccaaaactt ccggcaacga agcgcgcgaa cttgccgact tgagcgaaga catcggtatc
     2701 cgcttcaaat atccgaactc ggaacgcgtg tatctgcaag gcagccgcga cgacatccgc
     2761 gtgcctttgc gtgaaatccg tcaggacgac acctatacgg cgcaaggcgc ggaagccaac
     2821 ccgccgattc ccgtctatga caccagcggc gtgtacggcg atccagcggc acacatcgac
     2881 ctgaaacaag gtctgccgca catccgcacc gcatggctga acgaacgcgg cgataccgaa
     2941 atcctgccca agctctccag cgaatacggc atcgaacgcg cacacgatcc gaaaaccgcc
     3001 catctgcgct tcaaccaaat cacccgcccg cgccgcgcga aaagcggtag caacgtaacc
     3061 cagcttcact acgcgcgcca aggcattatc acgcccgaaa tggagtttgt cgccatacgc
     3121 gaacgtttaa aattagacga attgtcccaa aaatcggaat acgccaaact cttgaaacaa
     3181 cacgcagggc aaagtttcgg cgcgaacatc ccaacccgtc ccgaccaaat cacgcccgaa
     3241 ttcgtgcgcc aagaaatcgc cgccggacgc gcgattatcc ccgccaacat caaccacccc
     3301 gaactcgaac cgatgattat cggccgcaac ttccgcgtca aaatcaacgg caacttgggc
     3361 aattccgccg tcacttccag cctgaccgaa gaagtcgaaa aaatggtgtg gtcgctgcgt
     3421 tggggcgcgg acacaattat ggacttatcc acaggcgcgc acatccatga aacgcgcgaa
     3481 tggattatcc gcaacgcgcc cgtccccatc ggcaccgtgc cgatttacca agcgttggaa
     3541 aaaaccggcg gcattgccga agatttgact tgggatttgt tccgcgacac cttaatcgag
     3601 caggcggaac aaggcgtgga ctatttcacc atacacgcag gcgtgttgct gcgttatgtg
     3661 ccgatgactg ccgaccgcct caccggcatc gtatcgcgcg gcggttcgat tatggcgaaa
     3721 tggtgtttgg cacatcatcg ggaaaacttc ctttacacgc atttcgacga aatctgcgaa
     3781 attatgaagg cgtatgacgt atcgttcagc ctcggcgacg gtttgcgtcc cggctgcatt
     3841 gccgatgcca acgacgaatc ccaattcacc gagctgcaca ccttgggcga attgacctcc
     3901 aaagcgtgga aacacgacgt acaggtgatg atcgaaggtc ccggccatgt accgctgcaa
     3961 cgcgtcaaag aaaatatgac cgaagagttg caacactgtt ttgaagcacc gttttacacc
     4021 ctcggcccgc tcgttaccga catcgccccc ggctacgacc acatcacctc gggcataggc
     4081 gctgccaata tcggctggta cggcacggcg atgctctgtt acgttacccc caaagaacat
     4141 ttgggactgc ccgacaaaga agacgtgcgt accggcatca tcacctacaa actcgccgcc
     4201 cacgccgccg acctcgccaa aggctggccg ggcgcacagt tgcgcgacaa cgccctgagc
     4261 aaggcgcgtt tcgagttccg ctggcgcgac cagttccgcc taagcctcga ccccgaacgc
     4321 gccgaaagct tccacgacga aaccctgccc gccgaaggcg cgaaaatcgc ccacttctgt
     4381 tcgatgtgcg gccccaaatt ctgctcgatg aaaatcacgc aggaagtgcg cgactacgcc
     4441 gacaagcaaa aagcccaacg gcagggcatg gaggaaaaag cggtcgagtt cgtcaaaaaa
     4501 ggagcgaaga tttacagcta aaacgtcaag caaaaaatgc cgtctgaaac ccggaaaaaa
     4561 ggcttcagac ggcattcttt cgcttgtgaa aatatagtgg attaacaaaa atcaggacaa
     4621 ggcgacgaag ccgcagacag tac
//
LOCUS       DAWFAN010000145         4579 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWFAN010000145 DAWFAN010000000
VERSION     DAWFAN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.145"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            24..260
                     /locus_tag="WGO65_001682"
     CDS             24..260
                     /locus_tag="WGO65_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ0327956.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENPEIRSTLPLIANRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            428..2203
                     /gene="ggt"
                     /locus_tag="WGO65_001683"
     CDS             428..2203
                     /gene="ggt"
                     /locus_tag="WGO65_001683"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ0327957.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            2422..2617
                     /locus_tag="WGO65_001684"
                     /pseudo
     CDS             2422..2617
                     /locus_tag="WGO65_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2684..3934
                     /locus_tag="WGO65_001685"
     CDS             2684..3934
                     /locus_tag="WGO65_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ0327958.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKLGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            4062..4510
                     /locus_tag="WGO65_001686"
                     /pseudo
     CDS             4062..4510
                     /locus_tag="WGO65_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1261 a         1139 c         1059 g         1120 t
ORIGIN      
        1 aaaggcacgc cccagtttcc ccaatgtgga ttttcagcgc gtgcagtaga ggtgctgacc
       61 caattggcag accctttgct tttgttaata tcctagaaaa cccagaaatt cgcagtactc
      121 taccactcat cgccaatcgg ccaacctttc cacagctttg ggttaatggt gagttaatcg
      181 gtggtagtga tattatccta caaatgtacc aatcaggtga gctaaaacca ctcattgaag
      241 agcatagccc aaaggtctga ttacttaata tcttaaaata aatatcaaaa aagactgctg
      301 atgacattag cggtcttttt ttgatggtca agattgatag tttggattga attatttagc
      361 catattaaat acaataatca gcatgccttg tatgaatcat caatcaaact caggcgaagg
      421 agtgcttgtg gctaaaacat atttattgac tgcattgata atgtctatga caatctctgg
      481 atgtcaagtc atccatgcca atcaaggtaa ggttaatact cattctgctg tcatcacagg
      541 tgcagacgct cacacgcctg aacatgcaac gggactgacc gaacaaaagc aggtgattgc
      601 aagtgatttt atggtagcgt cagccaatcc attagcaaca caagctggct atgatatctt
      661 aaagcaaggc ggtagcgctg cagatgcgat ggtggcggtg cagacgacac taagcttggt
      721 agagccacag tcgtcaggct tgggcggtgg tgcatttgtg ttgtattggg ataataccgc
      781 caaaacattg accacatttg atgggcgtga gacggcaccg atgcgtgcga cgccggaatt
      841 atttttggat aaagatggtc aaccattgaa atttatggaa gcggtggtcg gtggtcgctc
      901 ggtgggtacg cctgctatcc ctaaactgat ggagacaata catcagcgat acggtgtatt
      961 gccttgggga aaattatttg atacaccgat ccgcttggca aaacaaggct ttgaggtgtc
     1021 gccaaggctt gccatctcgg ttgagcaaaa tcagcagcat ttggcacgct atccaaaaac
     1081 agccgcttat tttttgccga atggtgtgcc gctacaagca ggcagcttgc tgaaaaattt
     1141 agaatttgct gacagtgttc aggcgttagc agctcaaggt gcaaaagctc tgcatactgg
     1201 taaatatgcc caaaatatcg tttcagttgt ccaaaatgct aaggataacc ccggtcaatt
     1261 atccttacaa gatttatctg attatcaagt ggtggaacgc ccgcctgttt gtgtgactta
     1321 tcgtatttat gaagtatgcg gtatgggtgc accaagctca ggtgggattg ctgtgggtca
     1381 gattttgggg attttaaatg aattttcacc caatcaggtg gggtatgatg ctgaaggttt
     1441 acgtctcttg ggcgacgctt ctaggcttgc gtttgcggat cgtgatgtat atttgggcga
     1501 ccctgatttt gtaccagtac ccattcgcca gttgatttct aaagactacc taaaacatcg
     1561 cagccagctg cttgagcagt cggataaggc attgcctagc gtatctgcag gcgattttat
     1621 tcatgagtgg gtgtcctcac aagcgattga gttaccttct accagtcata tatcaattgt
     1681 ggataaggcg ggcaatgtgc tatcaatgac gacttccatt gaaaatgcgt ttggctcaac
     1741 tttgatggca aatggctacc tgctgaataa tgagctgaca gactttagtt ttgagccaat
     1801 aaagcagggc aaacaggtcg ccaatcgtgt cgagcccggc aagagaccac gctcctcaat
     1861 ggcaccaacc atcgtattta aagcaggcaa gccttatatg gcaatcggct ccccaggtgg
     1921 tagccgtatc attggctatg tcgctaagac gattgtggca catagtgact ggaatatgga
     1981 tatccaagat gccatcagtg cacccaattt attaaatcgc tttggtagct atgaactaga
     2041 aacgggtaca accgccctcc agtggcaaca agcattaaat gatttgggct ataagactga
     2101 cgtgcgtgag ctaaattcag gtgttcaagc cattattatc gagccaagca ggctggttgg
     2161 tggtgccgat ccacgccgag aaggtcgagt gatgggtgat tagaggtgaa gcttgtattt
     2221 tgtgaatttg accgcattaa gttaatatta aaataggggg aaattgtgag taatcaaaca
     2281 aaaatacaag ccatcaagca agcttctgag cagatcttcg caatttgtga gacgccaaac
     2341 acagcattac aagccattca tctgatttta agacatggtg gggcaggaga gttgtcttgg
     2401 caagtggtgt atcagcgggt tatggctgat aaagatgtgg ttggtgctgg ttatttgatt
     2461 gattttgcac aaactgccga aaatttacca tttgatgtat tgcctttggg cagccttgta
     2521 ctaacataaa ggtaatgaga cgctaaaaac cagcatgtta aataaattac ctgataatgc
     2581 caaagaaaat ttacgcatta tggactatct gccttaggtt ttgaagtatt tttttataca
     2641 aaaaacactc atgataccct gattacacaa aggagccgag aacatgtttt caaaaagcgt
     2701 taccctcgca caatatgacc ccgatttggc agcagccatt gcccaagaag accaacgcca
     2761 gcaagaccac gtcgagctga ttgcctctga aaactacgtc agctgcgccg taatggaagc
     2821 gcaaggttcc cagctgacca acaaatacgc cgaaggctac cccggcaaac gctactacgg
     2881 cggctgcgaa tacgtcgata ttgtcgaaca attggcgatt gaccgcgtca aaaaactgtt
     2941 cggcgcgcaa tacgccaacg tccaaccgca ctccggctcg caagccaacc aagccgtgta
     3001 cgcttccgtt ttaaaactag gcgacaccat tttgggtatg tctctagcgc acggcggcca
     3061 cctgacccac ggcgcgagcg ttaatatttc cggcaaactc tacaacgccg ttacttatgg
     3121 cttggatgaa aacgaagtgc tcgattatgc cgaagtggaa cgcttggctc tcgaacacaa
     3181 acctaaaatg attgtggccg gtgcgtctgc ctacgcgttg caaatcgact gggcaaaatt
     3241 ccgcgaaatc gccgataaag tcggcgcata cctctttgtc gatatggcgc actatgccgg
     3301 tctggttgcc ggcggcgaat atcccaatcc cgtgccgttc tgcgacttcg ttaccaccac
     3361 cacccacaaa accctgcgcg gtcctcgcgg cggcgtaatt ttgtgccgcg acaataccca
     3421 cgaaaaagcg ttgaactctt ccatcttccc aagcctgcaa ggcggtccgc tgatgcacgt
     3481 catcgccgcc aaagccgtag cgtttaaaga agcgttgcaa cccgagttca aacaatacgc
     3541 aaaacaagtg aaaatcaatg ctgccgccat ggcggaagaa ctggtcaaac gcggtttgcg
     3601 catcgtttcc ggccgcaccg aaagccacgt tttcctcgtc gacctgcaac cgatgaaaat
     3661 caccggcaaa gccgccgaag ccgctttggg caaagcgcac atcaccgtca acaaaaacgc
     3721 cattccgaac gatccggaaa aacccttcgt tacctccggc atccgcatcg gttccgccgc
     3781 catgaccaca cgcggtttta acgaagccga cgcgcgcgta ttggcgaact tggttgccga
     3841 cgtattgtct aaccccgaag acgaagccaa cctcgccaaa gtccgcgaac aagtaactgc
     3901 tttgtgcaat aaataccccg tttacggcgc gtaagccttt ttaaaaatat tccgccaagc
     3961 aatccaatgc cgcctgaaat ctcataatgt ttcaggcgga aacctttgca aaatccccca
     4021 aaatctccta aattcccacc aagacattta ggagatttct catgagcacc ttcttccggc
     4081 aaaccgcaca agccatgatt gccaaacaca tcgaccgctt cccactattg aagttggacc
     4141 aggtgattga ttggcagccg atcgaacaat acctgaaccg tcaaagaacc cgttacctca
     4201 gagaccaccg cggtcgtcct gcctatcccc tgctgtccat gttcaaagcc gttctgctcg
     4261 gacaatggca cagcctctcc gatcccgaac tcgaacacag cctcatcacc cgcatcgact
     4321 tcaacctgtt ttgacgtttt gacgaactga gcatccccga ttacagcacc ttatgccgct
     4381 accgcaaccg gcgacgcaag acgacaccct gtccgaattg ccggaactga tcaaccgcca
     4441 actgaccgaa aaaaacctaa aaatagagaa agcatccgcc gccgtcgttg acgccaccat
     4501 tattcagacc gagacctttg caaaattcct ttccctcccg acagccgaaa cccaaacaca
     4561 ggatttcggc tgttttcgt
//
LOCUS       DAWFAN010000146         4528 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWFAN010000146 DAWFAN010000000
VERSION     DAWFAN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.146"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(57..1850)
                     /locus_tag="WGO65_001687"
     CDS             complement(57..1850)
                     /locus_tag="WGO65_001687"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ0327959.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNTESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2095..3204
                     /gene="proB"
                     /locus_tag="WGO65_001688"
     CDS             2095..3204
                     /gene="proB"
                     /locus_tag="WGO65_001688"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ0327960.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3217..4479
                     /locus_tag="WGO65_001689"
     CDS             3217..4479
                     /locus_tag="WGO65_001689"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ0327961.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVTALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1039 a         1249 c         1247 g          993 t
ORIGIN      
        1 ccgcaatgcc gtctgaagcc tttttatgct tcagacggca tcgtctgttt tcacgctcaa
       61 atcgtaccgc tgccttgcgc tttcggtttg gcggcactaa attccagttt gctcaaagcg
      121 gaaaggtagg ctttggcagt ggcaaccaaa acgtcggtat ccgcgccctg accgttgacg
      181 acgcggttgc cgcgtgccag acggacgctg gtttcgcctt ggctttccgt accttgcgtg
      241 acggcattga cggaataaat ctgcaaagtc gcgccgcttt gcgccacgct ttcaatcgct
      301 ttgaaaattg catcaaccgg gccggaaccg gttgcggaag cgcgtttttc ttcgcctttg
      361 atgctgaaca cgatgtcggc gcgcggctct tcgccggttt cggtactgat tttttgggaa
      421 atgaatttgt agctttcggt attcatattg cccatttcgt ccgacaccag cgcgtgcagg
      481 tcttcatcga agatttcgcg ttttttgtcg gcgagttctt tgaagcgtgc aaatgccgcg
      541 ttcagtgcct cttcgctttc caactcgatg cccaaatccg ccagcttggt tttgaaggcg
      601 ttgcggccgg acaatttgcc caagctcaat cggttagccg accaaccgac cgattcggca
      661 gacataatct cgtaagtttc gcggtgtttc agcaccccgt cctgatggat gcccgattca
      721 tgcgaaaagg cattggcacc gacaatggct ttgttgggct gcacgggata gcccgtaatg
      781 gtggacacca gtttggacga aggcacgatt tgcgtggtat cgatgccggt ttccaagccg
      841 aacaagtcgt ggcgcacttt caacgccatc acgatttctt caaccgaagc attgcctgca
      901 cgttcgccca agccgttgac agtacattcc acctgacgcg cgccgccttt taatgcggca
      961 agcgaattgg caaccgccaa gcccaaatca ttgtggcagt gtgccgacca aacgactttg
     1021 ccgccgttgg gcgttttggc aatcagttcg cggaaaaatt cttcggtttt gtacgggatt
     1081 gaatagccga cggtatcggg aatattgatg gtggtcgcgc ccgcttcaat caccgcgccg
     1141 cagatttcgg caaggaaatc gatttccgaa cgcaacgcgt cttcgcagga aaattccaca
     1201 tcgtcggtgt attcacgagc gattttcacc gctttgaccg ccgcctcaat cacctgcttc
     1261 ggcttcatct tcaatttgta ctccatatgg atggggctgg tggcgatgaa ggtgtggatg
     1321 cgttttttcg gcgcgggcgc aacggcctca cccgcctgac ggatgtcccg ctcgatggcg
     1381 cgggacaatg aacagaccgt tgatttggta atggttttcg caatcgcatt gaccgcctcg
     1441 aaatcgcccg ggctggcagc ggcaaaaccc gcttcgatga tgtccacacc caatttttcc
     1501 agctggcggg cgacgcggat tttttcctct ttggtcatag cggcgccggg cgactgctcg
     1561 ccgtcgcgca gggtggtatc aaaaataatt acgcggttgg tctgtgtcat ttctgttctc
     1621 tccagagatt cgtttttttg taaaaaagca ttcaaatcca gcggcctgcc ttgcgcttcc
     1681 gccaacgcgg tcagcttttg cagttgcgcc agaggcagcg agccgcgcgt gcgccattta
     1741 tagatggcgg cggtcgtcgc ggcattttcg ggatcgtgct gtttcaacgc ttcggcaagc
     1801 gcgttcacgc cgccgaaata agcaaccaag cggtctatat ctaattgcat ggtagtcctt
     1861 gtctaaaata aatcagccta cagcagaaat atggacagga ttatacgcat tttggacaaa
     1921 acggcaatat aaatttgaaa aatctcaata agccgccata gattttatta attttataca
     1981 ttttgtccat tttattgcag acgcggcgtg attgttgaca attgcggcag aacagggcaa
     2041 attcggcggc acaaggtggc aacgagacag gacaaaccaa caaggacgga aacaatgaaa
     2101 tacaaaagaa tcgtatttaa agtcggcaca tcttcgatta cccattcgga cggcagtctc
     2161 tcgcgcggca aaatccaaac catcacccgc cagcttgccg cattgcatca tgcgggacac
     2221 gagctggtct tggtgtcttc cggcgcggtt gcggcagggt tcggtgcgct gggtttcaaa
     2281 aaacgtccgg tcaaaatcgc cgacaaacag gcttccgccg ccgtcgggca ggggctgctg
     2341 atggaagaat atacggcaaa cctgtcttca gacggcatcg tgtccgcaca aatcctgctc
     2401 agccgcgccg actttgccga caaacgccgc taccaaaatg ccggcggcgc actttccgtg
     2461 ctgctgcaac gccgcgccgt ccccatcatc aatgaaaacg atacggtttc ggttgaggaa
     2521 ttgaaaatcg gcgacaacga cacattgagt gcgcaagtgg cggcgatgat acaggcagac
     2581 ctcttggtgc tgctgaccga catagacggt ctttacaccg gcaacccgaa cagcaatccc
     2641 gatgccgtac ggctggacaa aatcgaacac atcaaccatg aaatcatcga aatggcgggc
     2701 ggatcgggtt cggcaaacgg cacgggcggt atgctgacca aaatcaaagc ggcaaccatc
     2761 gccgccgaat ccggcgtacc ggtgtatatc tgttcctcgc tcaaacccga tgcacttgcc
     2821 gaagctgccg aacatcaggc ggacggctcg tttttcgtcc cccgtgccaa aggtttgcgg
     2881 acgcagaagc aatggctggc gttctattcc gaaagccggg gcagcgttta tgtggacgaa
     2941 ggtgcggaac acgctttgtc cgaacagggg aaaagcctgc tgatgtcggg cattgccgga
     3001 atcgaagggc atttttcccg tatggacacc gtaaccgtgt acagcaaggc aaccaaacag
     3061 cccctgggca aagggcgcgt cctgttcggt tctgccgccg ccgaagacct gctcaaatcg
     3121 cgtaaggcga aaggcgtgtt catccatcgg gacgactgga tttccatcac gcccgaaata
     3181 cgcctgcttc tgaccgaatt ttagaaaagg aaacccatgt caaacacaca aaaacagctt
     3241 gcccttgcca aagcggcaaa aaaatccgtc aacacggcgg atacggaaga aaaaaaccgc
     3301 gcgctgcttg ctatggcgga cagtctggaa gcggcggcgg cagatatttt ggcggcaaac
     3361 cgtcaagact tggaagccgc ggcaggcaac attcccgaaa gcatgaccga ccgccttttg
     3421 ttggacggca aacgcatttg cgcgatggcg gacggcatta gggcggttac cgcactgccc
     3481 gaccccgtgg gcgaaatact ggaaacctcg actctgccca acggcttgga aatcgtcaaa
     3541 aagcgtgtgg cgatgggcgt tatcggcatt atttacgaaa gccgcccgaa cgttacgtcc
     3601 gatgcggcgg ctttggcact gaaaagcggc agcgcggtcg tactccgcag cggcaaagat
     3661 gcattccaat ccgcacgcgc catcgttgcc gccctgaaaa cggggttggc acaaacccgc
     3721 atcgaccccg acgcgctcca gttgattgaa gacaccgggc gcgagggcag ttacgaaatg
     3781 atgagggcga aagattatct agacctgctg attccgcgcg gcggggcggg gctgatacgg
     3841 gcggtggttg aaaatgccgt cgtgccggtc attgaaacgg gaacgggcat cgtccacatt
     3901 tatatcgaca aggacgcgga ttgggacaag gcgctccgta tcgtgtacaa cgccaaaacc
     3961 agccgtccgt ccgtgtgcaa ctcgatggaa gtgctgctgg tgcatgaaga cattgctgcc
     4021 gacttcctgc ccaagctcga acggctgttg gttcgcgacc gtatagaagc cggactgccg
     4081 cccgtccgct tccgtttgga tccgcaggcg gcgcggcata tcggcggcga agcggcgggt
     4141 gcagacgatt tcgataccga gtttttagac tacatcctcg ccgtgaaaac cgtcgcttcg
     4201 gtcgaagagg cggtcgggca catcgaaacg cacggcacgc accattcaga cggcatcgtt
     4261 accgaaaacc gccacgctgc ggactatttc acgacccata tcgattctgc cgccgtgtat
     4321 atcaacgcgt ccacgcgctt taccgacggc ggcgaattcg gcttgggttg cgaaatgggc
     4381 atctccacgc aaaaactcca cgcccggggc ccgatgggtt taaaagagct gacgagttac
     4441 aaatacatcg tacaaggcac gggacaggtt agggaataat ccgaattcaa acgtctgata
     4501 tagtggatta actttaaacc gatacggc
//
LOCUS       DAWFAN010000147         4523 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWFAN010000147 DAWFAN010000000
VERSION     DAWFAN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.147"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            40..463
                     /locus_tag="WGO65_001690"
                     /pseudo
     CDS             40..463
                     /locus_tag="WGO65_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            569..1132
                     /gene="pgsA"
                     /locus_tag="WGO65_001691"
     CDS             569..1132
                     /gene="pgsA"
                     /locus_tag="WGO65_001691"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ0327962.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1212..1287
                     /locus_tag="WGO65_001692"
     tRNA            1212..1287
                     /locus_tag="WGO65_001692"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1245..1247,aa:Gly,seq:gcc)
     gene            1313..1388
                     /locus_tag="WGO65_001693"
     tRNA            1313..1388
                     /locus_tag="WGO65_001693"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1346..1348,aa:Gly,seq:gcc)
     gene            1412..1487
                     /locus_tag="WGO65_001694"
     tRNA            1412..1487
                     /locus_tag="WGO65_001694"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1445..1447,aa:Gly,seq:gcc)
     gene            1509..1584
                     /locus_tag="WGO65_001695"
     tRNA            1509..1584
                     /locus_tag="WGO65_001695"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1542..1544,aa:Gly,seq:gcc)
     gene            1610..1683
                     /locus_tag="WGO65_001696"
     tRNA            1610..1683
                     /locus_tag="WGO65_001696"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1642..1644,aa:Cys,seq:gca)
     gene            1786..1875
                     /locus_tag="WGO65_001697"
     tRNA            1786..1875
                     /locus_tag="WGO65_001697"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1820..1822,aa:Leu,seq:taa)
     gene            1979..3031
                     /gene="sohB"
                     /locus_tag="WGO65_001698"
     CDS             1979..3031
                     /gene="sohB"
                     /locus_tag="WGO65_001698"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ0327963.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDHLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3082..4007)
                     /locus_tag="WGO65_001699"
                     /pseudo
     CDS             complement(3082..4007)
                     /locus_tag="WGO65_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201178.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
     gene            complement(4061..4492)
                     /locus_tag="WGO65_001700"
     CDS             complement(4061..4492)
                     /locus_tag="WGO65_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0327964.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRKRLLSLTETGKAYAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1134 a         1069 c         1175 g         1145 t
ORIGIN      
        1 tatcgctggg tatggtttgc cgccataagc tgtctgctgt tgtccgccgt ttttattgca
       61 ccttacctga cggcatttca cgaacaagaa aaaacattcg aatatgcgga attgaccgtt
      121 accgccctca accgcagcgg acgggcaatc aaactggatg cagacgggca gcattaccgg
      181 ctttcctgct atgggttcga cagtttgtgc acaggcggca atatcggcag aaccatcagg
      241 gcggagcagg ttagagccgt tttaagcgaa accgtcggca aaggtttttt aaacggcgtt
      301 ctgttggaat accgcaacag tggcagtgtc tataagcaat aaagattttg cccgcacgga
      361 agaccgcctt gtcgaagtgt tggcacaacc tgccgttttc tgcctgaaac tgggtatttt
      421 gcttttattg cccgccattt tcctgcgttt gaaaagaatg tgaaaacaga ttggcggggc
      481 gggggaatgc ccatgcagca attttacgtt ttgggttatg aggataaccg ttataatcac
      541 aactttacag tctgcacaga gaaaaccgat gccttggaat ctccccatct tcctcacatg
      601 gttgagggtc ttgctcattc ctgtcatgac agcccttttt tacctgcctt ttccgtggtt
      661 cgcggaggaa acgattaatc tcaccgccgc cgtcattttt gccgttgccg ccttaaccga
      721 ctggtttgac ggatttttgg caagactgtg gaaacagact tccgatttcg gcgccttcct
      781 cgatcccgtc gccgacaagc tgatggttgc cgtatcgcta cttttactgg tcaaactcga
      841 ccggacctat gttttgtttg ccatgatcat tatcggcagg gaaattacca tttccgcatt
      901 gcgcgaatgg atggcgcaaa tgggcaaaag gaacagcgtt gccgtcgcca ccgtcggtaa
      961 gttcaaaacc gccgcgcaaa tgctggcaat tttcctgctg ctgctgaata ttcccgattt
     1021 ttacggattt aatcttgttg ttatcggcaa catattgatg tttatcgcat ctttgctgac
     1081 agtctggtcg atgctgtatt atctgaaaat ggcgtggaaa gaaatcgcct gaaaaaaaca
     1141 taaaaatagc ttgacggcaa aaacagaatc cataataatt gcgtctcttc gatgttggag
     1201 aatgaaatcg ggcgggaata gctcagttgg tagagcgcaa ccttgccaag gttgaggtcg
     1261 cgagttcgag actcgtttcc cgctccaaaa atttcatttt cccacaatca ttgcgggaat
     1321 agctcagttg gtagagcgca accttgccaa ggttgaggtc gcgagttcga gactcgtttc
     1381 ccgctccaag ttttatttca aacatatcaa cgcgggaata gctcagttgg tagagcgcaa
     1441 ccttgccaag gttgaggtcg cgagttcgag actcgttttc cgctccaaaa accgatatgt
     1501 ttgaacgcgc gggaatagct cagttggtag agcgcaacct tgccaaggtt gaggtcgcga
     1561 gttcgagact cgtttcccgc tccaagtttt ttagataagc cagttttagg gcgagatagc
     1621 aaagtggtta tgcagcggat tgcaaatccg tctacgccgg ttcgattccg actctcgcct
     1681 ccattatcgt actatggcgg ggtggcagag tgtttatgct atgaggagtg caatcttcat
     1741 ataggccggt taaaatccgc gcccccgcct ccacctttca caaatgcccg ggtggtgaaa
     1801 taggtagaca caacggactt aaaatccgtc gggactaaac atcccgtgcc ggttcgattc
     1861 cggctccggg caccaagctg gaaatgacaa taccgctcca agcggttatt tttttatctg
     1921 tcggacggtg atggacaata ttgacatgtt catgcctgaa caagaggaaa tccaatcaat
     1981 gtggaaagaa attttactga attacggtat tttcctgctc gaactgctta ccgtgttcgg
     2041 cgcaattgcg ctgattgtgt tggctatcgt acagagtaag aaacagtcgg aaagcggcag
     2101 tgtcgtactg acggattttt cggaaaatta taaaaaacag cggcaatcgt ttgaagcatt
     2161 ctttttaagc ggggaagagg caaaacatca ggaaaaagag gaaaagaaaa aggaaaaggc
     2221 ggaagccaaa gcagagaaaa agcgtttgaa ggagggtggg gagaaatctg ccgaaacgca
     2281 aaaatcccgc ctttttgtgt tggattttga cggcgatttg tatgcacacg ccgtagaatc
     2341 cttgcgtcat gagattacgg cggtgctttt gattgccaag cctgaagatg aggttctgct
     2401 tagattggaa agtccgggcg gcgtggttca cggttacggt ttggcggctt cgcagcttag
     2461 gcgtttgcgc gaacgcaata ttccgctgac cgtcgccgtc gataaggtgg cggcgagcgg
     2521 cggttatatg atggcgtgtg tggcggataa aattgtttcc gctccgtttg cgattgtcgg
     2581 ttcggtgggt gtggtggcgg aagtaccgaa tatccaccgc ctgttgaaaa aacatgatat
     2641 cgatgtggat gtgatgacgg cgggcgaatt taagcgcacg gttactttta tgggtgaaaa
     2701 tacggaaaag ggcaaacaga aattccgaca ggaactggag gaaacgcatc agttgttcaa
     2761 gcagtttgtc agcgagaacc gccctcaatt ggatattgag gaagtggcaa cgggcgagca
     2821 ttggttcggt cggcaggcgt tggcgttgaa cttgattgac gagatttcga ccagtgatca
     2881 tttgttgttg aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc aggagaagca
     2941 aagcctgatc cagcgcattg gtttgcaggc ggaagcttct gttgaaaagt tgtttgccaa
     3001 acttgtcaac cggcgggcgg atgtgatgta gtttgccgaa cggatggaaa tgccgtctga
     3061 agcgtgttca gacggcattt ttcaagttcc ggctttgatg ttccgttccg accactctat
     3121 ccgttgtccg atgtctttca aattggaata ttgttcgacc aatcgcggca cgtcgtccaa
     3181 actcatggca aacatccaca gataagtgcc gaccgaatag acatgccccg cgctgctgta
     3241 gcctttgagc gtcatcatca caaaagcaaa gccgaacaaa atacccatcg ccgtgccgac
     3301 gcagagatag ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg caagcagtcc
     3361 gtaatggcgg tacagctgcc gccggtcgcc ttttcggata aagtggttgt cgcgttccaa
     3421 gctgttcttc aggcggaaat acaggttttc gctgatggcg gcaaaacgtg gcaaaagcca
     3481 taaaaacaac gcaagtatgc ccaccgccga cacgccgacc caaaattcca gcaccagcag
     3541 catgatgcac gcgccgaata tggatacgac ggatgtcgcg gcaatcggca ggtgttcttc
     3601 aaaaaagctg acaaactcac gcgacagggc aacccgtgca gttatagtgg attaacaaaa
     3661 atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggcaaggc gagacaacgc
     3721 cgtactggtt taaatttaat ccactatatt gccgaatgcg ggacttaccg ctgccgctgt
     3781 tccaacacaa ccggcacggc gatttcggta taaatccgcg taaacgtgcg tgtatcggca
     3841 atccgccgcg ccgcaccgac cagccacatt aaaagcacaa ccaaagcgta cagcaacgcc
     3901 tgccacacct tccccgcaat tacggcatta attgcccagc cgccaaacac cggatacacc
     3961 agcatcaaaa ggttttccag tccgaccaga aaaaatgtgc caatcaatcg cttgcggtgg
     4021 ttttggctat gtgtttcaac attttccaca tctttgctcc ctatttttta ttttccgaga
     4081 ttgttttttc catcacttcc gccagtgcat ccaaatcggc aaacagccga gttgtgcgct
     4141 tgtcgccgaa tgtggcaaat actttgtcgc tgaattcctg cgcgctttct gttaaaggtg
     4201 cggcataggc tttgcctgtt tcggtcaacg acagcaaccg tttgcgccgg tcctgttcgc
     4261 cttcctgcca ttcaatcaac ccttgtccgg caagggtttt gcatacgcct gaaacggtct
     4321 gtttgggcag gctccacttt tcgccgatat gcttttgcgt gcggctgcct tcggttgcca
     4381 gggtataaag tacggcaaag aggttgtaat tcagatcctg ctgcccaatc catttgtcga
     4441 agacattgca aatcaggttg atacgggtgc caagttggtc gagttggttc ataattggtc
     4501 ttgatattga ctaaatttgt gcg
//
LOCUS       DAWFAN010000148         4522 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWFAN010000148 DAWFAN010000000
VERSION     DAWFAN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.148"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..251)
                     /locus_tag="WGO65_001701"
     CDS             complement(<1..251)
                     /locus_tag="WGO65_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
                     /protein_id="HEZ0327965.1"
                     /translation="MEDKQGMTKAVVGVMTDALADGRKPATASNLPPLSNRRGTETET
                     AGRVQEVFECYEMYITDGKGNLLGVPLRRGVSDSAFIDQ"
     gene            complement(323..568)
                     /locus_tag="WGO65_001702"
     CDS             complement(323..568)
                     /locus_tag="WGO65_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327966.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(569..865)
                     /locus_tag="WGO65_001703"
     CDS             complement(569..865)
                     /locus_tag="WGO65_001703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327967.1"
                     /translation="MLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFS
                     DGEYRYVDVLQPNHSDIIRYSGKDFPINQILNHIHLARYAVTFENNVDSKLRRH"
     gene            complement(856..1209)
                     /locus_tag="WGO65_001704"
     CDS             complement(856..1209)
                     /locus_tag="WGO65_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327968.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            1445..2872
                     /locus_tag="WGO65_001705"
     CDS             1445..2872
                     /locus_tag="WGO65_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ0327969.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            2941..4344
                     /locus_tag="WGO65_001706"
     CDS             2941..4344
                     /locus_tag="WGO65_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ0327970.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
BASE COUNT         1145 a         1113 c         1183 g         1081 t
ORIGIN      
        1 atttggtcga tgaaagccga atcggataca ccgcgcctaa gaggaacgcc taacaggttt
       61 cctttaccgt ccgttatgta catttcgtaa cattcgaaga cttcctgaac cctgcctgcc
      121 gtttcggttt ctgtacccct cctgttagat aaggggggaa gatttgaagc ggttgccggc
      181 ttcctgccgt ccgctaacgc gtccgtcatc acgccgacaa ccgcctttgt catcccttgc
      241 ttatcttcca tggtgcgaat cctcaaaaac aggcaaaaaa aaagccctgt tacttgtaga
      301 aagtaaagga cgttaatttt tgttaatcgt cccttcttag ggacgcaata tataagggac
      361 gcaatatata aggttttaaa acgctaagaa caaaatccga taacggctct gaagaagtaa
      421 gtctttcttt attgcacaag atagcaaact tccacggcat tctcgcccca cccgtgccgt
      481 ccaagccttt ttcaaagaca tagatggttt tcttggcaat cacttcgtca gtttggcggt
      541 cgataatccg tatggtcgca cctgccactc agtgcctgcg cagcttggaa tcgacattgt
      601 tttcgaacgt taccgcataa cgggcgaggt gtatatgatt aagtatttga tttatcggaa
      661 aatctttacc tgaataccga ataatatcgg aatggttggg ttgcaggaca tccacatagc
      721 ggtattcccc atcgctaaaa aatcctagga aacgagcaat aaaatttact ccttcgctgg
      781 tctgtaaagc cgcattgtcc cacatcgggt ctctggtttt tgcatctgcc gaaacggtac
      841 gctcaggtac taccttcaac agcataatcc cttccacatt gtccgccgtc tggtaaatct
      901 ttcccccgcc gttttgcatt gttcgttaaa cacggcttcg gcttctttgt attttctgtc
      961 ccactcttct tgtgcctgta tttcttcttt gatcgggccg aattgtttgg gaataatcca
     1021 aacaaacagc atcaggatag cggcggcggt caggctgcct gaaaggattt tgccggggtt
     1081 ccgtttgggc tttttatagg caaagcggac gagaaaccaa agcaacagca gcatggtgcc
     1141 ccaatagccg attgagaata ggatggccaa accttctagg aaatggcgta aatcgtttgt
     1201 ggtaaacatg ggttgttcct gtggttaaat gtgcaggctg ctttttgccg aatcttgccg
     1261 catctcaaaa gcagcctgcg cttcagcgtt gcgttacgca gtaaaataat gaatatttgt
     1321 aacgacttgg gtattttttg tcaatattcc cgcctttccc ttaacagctg ccgcgctttc
     1381 cgttaaaatt cctttacata tttatattgt ttcctgtttc tatattgcca aggttatacc
     1441 cgttatgttt ttctccgccc tgaaatcctt tctttctcga tacattactg tatggcgcaa
     1501 tgtttgggcg gtgcgcgacc agttggaacc gcccaaacgc acggcggaag aacaggcgtt
     1561 tttgcccgcg catttggaac tgaccgatac gccggtctct gccgctccga aatgggcggc
     1621 gcgttttatt atggcgtttg cgcttttggc tttgttgtgg tcctggttcg gcaaaatcga
     1681 tattgtggcg gcggcttcgg gcaaaacggt gtcgggcggg cgcagcaaaa ccatccagcc
     1741 gctggaaacg gcggtggtta aggcggtaca tgtgcgcgac gggcagcatg tgaaacaggg
     1801 agaaacgctg gcggaactgg aggctgtggg aacagacagc gatgtggtgc agtcggagca
     1861 ggctttgcag gctgcccaat tgtccaaact gcgttatgaa gcggtattgg cggcattgga
     1921 aagccgtacc gtgccgcata tcgatatggc gcaagcacgg tctttaggtc tctccgatgc
     1981 cgatgtgcaa tcggcgcagg tgttggcgca gcaccagtat caggcatggg cggcgcagga
     2041 tgcgcaattg cagtcggctt tgcgcggcca tcaggcggaa ttgcagtcgg ccaaggcgca
     2101 ggagcagaag ctggtttcgg tgggggcgat cgagcagcag aaaacagcag actaccgccg
     2161 tttgcgggcc gacaatttta tttcggaaca tgcgtttttg gagcagcaga gcaaatcggt
     2221 cagcaattgg aacgatttgg aaagtacgcg cggtcagatg aggcagattc aggcggccat
     2281 tgcacaggcg gagcagagtc gggtgctgaa tacgcagaac ctgaaacgcg atacgctgga
     2341 tgcgctgcgc caggcaaacg aacagattga ccaataccgc ggccaaacgg ataaggcaaa
     2401 gcagcggcag cagctgatga caatacagtc gcctgcggac ggcacggtgc aggaattggc
     2461 cacctatacg gtgggcggtg tggtgcaggc tgcccaaaaa atgatggtgg ttgcgcccga
     2521 tgacgacaaa atggacgtgg aagttttggt attgaacaaa gacatcggtt ttgtggaaca
     2581 gggacaggat gcggtggtga agattgagag ttttccctat acgcgctacg gttatctgac
     2641 gggcaaggtg aaaagtgtca gccatgatgc ggtaagccac gaacagttgg gcttggttta
     2701 tacggcggtg gtgtcgctgg acaaacatac cttgaatatt gacggcaaag cagtgaatct
     2761 gacggcgggc atgaatgtca cggcggagat taaaacgggt aaacggcggg tgctggatta
     2821 tctgttaagc ccgctgcaaa ccaaattgga cgaaagcttt agggagcgat aggcggatcc
     2881 gtactgggca tttgttatcc gccggttcgg acatgcagac tgcctgaaac cattgcccgg
     2941 atgacattgc tcaatctaat gataatgcaa gattacggta tttccgtttg cctgacactg
     3001 acgccctatt tgcaacatga actattttcg gctatgaaat cctatttttc caaatatatc
     3061 ctacccgttt cactttttac cttgccacta tccctttccc catccgtttc ggcttttacg
     3121 ctgcctgaag catggcgggc ggcgcagcaa cattcggctg attttcaagc gtcccattac
     3181 cagcgtgatg cagtgcgcgc acggcaacaa caagccaagg ccgcattcct tccccatgta
     3241 tccgccaatg ccagctacca gcgccagccg ccatcgattt cttccacccg cgaaacacag
     3301 ggatggagcg tgcaggtggg acaaacctta tttgacgctg ccaaatttgc acaataccgc
     3361 caaagcaggt tcgatacgca ggctgcagaa cagcgtttcg atgcggcacg cgaagaattg
     3421 ctgttgaaag ttgccgaaag ttatttcaac gttttactca gccgagacac cgttgccgcc
     3481 catgcggcgg aaaaagaggc ttatgcccag caggtaaggc aggcgcaggc tttattcaat
     3541 aaaggtgctg ccaccgcgct ggatattcac gaagccaaag ccggttacga caatgccctg
     3601 gcccaagaaa tcgccgtatt ggctgagaaa caaacctatg aaaaccagtt gaacgactac
     3661 accgacctgg atagcaaaca aatcgaggcc atagataccg ccaacctgtt ggcacgctat
     3721 ctgcccaagc tggaacgtta cagtctggat gaatggcagc gcattgcctt atccaacaat
     3781 catgaatacc ggatgcagca gcttgccctg caaagcagcg gacaggcgct tcgggcagca
     3841 cagaacagcc gctatcccac cgtttctgcc catgtcggct atcagaataa cctctacact
     3901 tcatctgcgc agaataatga ctaccactat cggggcaaag ggatgagcgt cggcgtacag
     3961 ttgaatttgc cgctttatac cggcggagaa ttgtcgggca aaatccatga agccgaagcg
     4021 caatacgggg ccgccgaagc acagctgacc gcaaccgagc ggcacatcaa actcgccgta
     4081 cgccaggctt ataccgaaag cggtgcggcg cgttaccaaa tcatggcgca agaacgggtt
     4141 ttggaaagca gccgtttgaa actgaaatcg accgaaaccg gccaacaata cggcatccgc
     4201 aaccggctgg aagtaatacg ggcgcggcag gaagtcgccc aagcagaaca gaaactggct
     4261 caagcacggt ataaattcat gctggcttat ttgcgcttgg tgaaagagag cgggttaggg
     4321 ttggaaacgg tatttgcgga atagagcagg ctgaaacggt tatgaaattc ccaaagcagc
     4381 ctgcaccccg tttcgaaagt gcaggctgct ttgggattga tccgatactt tcacattctc
     4441 attgccttta aataaagcac agttaaaaac acatttaaat taagtgcgaa ttaattcaaa
     4501 tgtatattca attaaaatca aa
//
LOCUS       DAWFAN010000149         4487 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWFAN010000149 DAWFAN010000000
VERSION     DAWFAN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.149"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(333..578)
                     /locus_tag="WGO65_001707"
     CDS             complement(333..578)
                     /locus_tag="WGO65_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327971.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            690..1118
                     /locus_tag="WGO65_001708"
     CDS             690..1118
                     /locus_tag="WGO65_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ0327972.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1130..1606
                     /locus_tag="WGO65_001709"
     CDS             1130..1606
                     /locus_tag="WGO65_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ0327973.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISELSRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(1956..2444)
                     /locus_tag="WGO65_001710"
     CDS             complement(1956..2444)
                     /locus_tag="WGO65_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ0327974.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2512..3567)
                     /gene="aroG"
                     /locus_tag="WGO65_001711"
     CDS             complement(2512..3567)
                     /gene="aroG"
                     /locus_tag="WGO65_001711"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ0327975.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWDATEE
                     LLALLAGANKKRMARAG"
     gene            complement(3729..3956)
                     /locus_tag="WGO65_001712"
     CDS             complement(3729..3956)
                     /locus_tag="WGO65_001712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327976.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            3892..4124
                     /locus_tag="WGO65_001713"
                     /pseudo
     CDS             3892..4124
                     /locus_tag="WGO65_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4183..>4487
                     /locus_tag="WGO65_001714"
     CDS             4183..>4487
                     /locus_tag="WGO65_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327977.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKS"
BASE COUNT         1074 a         1148 c         1051 g         1214 t
ORIGIN      
        1 gacgggattt caggttgctg tttttgattt tctgttttgt gggaataacg ggattttgga
       61 tgatgtgcat tttcgggaaa actaaaaacc ctccgccgtc attcccgcga aagcgggaat
      121 ctagaatttt aatgcggcaa aaatttatcg gaaaaaaccg aagtttaaag acctagattc
      181 ccgcctgcgc gggaatgacg gctcaaaagt tacgaaacga aaaataaccg aaaccaaaca
      241 agtcggattc ccgcgcgggc gggaatgacg ataagcgtaa tttcaatctg ccatattgtc
      301 ttttcacttg cctgaaaaca gatttcagcc acctatccct gcatccggcg gctgatgtag
      361 tcggcaatca aatcccgctt ttcccaaaaa aacttctgca ttttttgttc ggaatacagt
      421 ttgcggacca gagtgggcaa accgctgtgg acgatggcgg cgatttcttt gttgcctttt
      481 tcttcctgca ttgccgtctg aaaatccata cccctgcctt tcagatattc tgcaaggcgg
      541 ttgcggatgt gggaattgag ttcgatggat ttgagcattt tgcctccgtt ttgcggtaag
      601 gatttattcg atttgtttat ccgcctgatt ttatcacgtt tcccaagcgg tttgcctgta
      661 caataccgct tttcggcagg gtgtgggcga tgatgttttc ttggttcaag ctgtttcact
      721 tgttttttgt catttcgtgg tttgcagggc tgttttacct gccgaggatt ttcgtcaata
      781 tggcgatgat tgatgtgccg cgcggcaatc ccgagtatgt gcgtctgtcg ggcatggcgg
      841 tgcggctgta ccgttttatg tcgccgttgg gcttcggcgc ggtcgtgttc ggcgcggcga
      901 taccgtttgc cgccggctgg tggggcagcg gctgggtaca cgtcaaactg tgtttgggct
      961 tgatgctctt ggcttaccag ttgtattgcg gcgtgctgct gcgccgtttt caggattaca
     1021 gcaatgcttt ttcacaccgc tggtaccgcg tgttcaacga aatccccgtg ctgctgatgg
     1081 ttgccgcgct gtatctggtc gtgttcaaac cgttttgacg gagcatctta tgccgataga
     1141 aatcgaacgc cgttttttga ttggaaacga caactggcgg caatacgccg acgaacctct
     1201 gctgttgagg cagggttatc tgtctgttga aaaagaacgc accgtccgtg tccgtattgc
     1261 cggacaacgg gcgtggctga cgctgaaagg ctatatttcg gaactcagcc gcagtgagtt
     1321 tgaatacgaa atcccgcttg ccgatgcgga aaagatgatg gaaacgatgt gtccgtttaa
     1381 gatggaaaaa tggcgttatc cggtcagatg gggcggcagc ctgtttgaaa tcgatgtttt
     1441 tcttggcgat aatgcgcctt tggtcgtcgc ggaaatcgag ttgcccgacg agaatgccga
     1501 ttttgaccgt ccggactggc tgggacggga aatcactgcc gacggtatgt ttaccaatgc
     1561 ctatttgagc aggcatccgt tctcaagctg gaaaaatgcc gtctgaagcg gcttcagacg
     1621 gcattctccg acaaccatcg caaatccaaa gacagcctct atatccttat ttctgctttc
     1681 tttgttccat tattcccaat aaatccccat aacctaaaat ctcgtcattc ccgctcaggc
     1741 gggaatccgg ttctttaaac ttcggttttt tccgataaat tgctttagct tttcgtttcc
     1801 ggattcccgc ttttgcggga atgacgatgg aagattgttg ttatttcgga taaattaccc
     1861 taactttaaa atcccggatt cccatcttcg ggggaataac aatattttct atttcagccc
     1921 atcatttgcg ggggatttta tagagagttt atatttcaat atctcaaata atgcacaaaa
     1981 gcatagcgcg tgcctttgct gctgacacgg cgttccgtcc gctctgcttc tttccaatgc
     2041 gtccggtcta ttgcggggaa aaatgcatct ccttccacag acaaatccac ttcggttatc
     2101 cgcaaatcgg tcgccaatgg catcgcttgt ccgtatatct gcgcgccgcc cataatgacg
     2161 gcttcttccg cgcctgcgca caatgccaat gccgcctcca aacttgccgc cgtttccgcg
     2221 cctgccgcgc aataatccgc ctgccggctg atgacgatgt tcctccgtcc aggcaggggt
     2281 ttgacgggca gggattccca cgttttccgc cccataatga cgggtttgcc caaggtatag
     2341 gctttgaaaa atgcgaaatc ttcggggatg tgccaaggca tagcattgcc cgccccgatg
     2401 cacaggtttt ccgcacacgc cgcaattagg gttattttga gcatatttcc tccacaaaaa
     2461 tgccgtctga acgcttcaga cggcatttta tgacattctg cgtcaaaaat ctcaaccggc
     2521 gcgcgccata cgttttttgt ttgcacctgc caacaatgcc aacagttctt cagtcgcgtc
     2581 ccaaccaata cacgcatcgg taatgctttt gccgtacact tccggcttgt cctgcctgcc
     2641 ttcgaccaaa tggctttcca ccatcacgcc cataatattg ccgccgtcct gttccaattg
     2701 ggcggcaatg tcttgcgcca cttccatctg ccgggtgtaa tccttgcggc tgttggcgtg
     2761 gctgcaatct atcatcagct tatcggttac ccctgccgca cgcagttgtt ccgccgcctc
     2821 gctgacgtgt cccgcatcat aattcggctc ttttccgccg cgcaaaatga catgacagtc
     2881 gggattgccg ccggtatgga caatggcgga atgtccggcc ttggttacag acaagaaatg
     2941 atgcgaatgg ctcgccgcac cgattgcgtc gatggcaatc ttcaaattgc cgtccgtacc
     3001 gtttttaaag ccgacggggc aggacagccc gcttgccaat tcgcggtgaa cttggctttc
     3061 ggtcgtccgc gcaccgattg ccccccaaga aatcaagtcc gcataatatt gcggcgtaat
     3121 catatccaaa aactcggtag aggcaggcat acccatattg ttcagcgaca acaacaggct
     3181 gcgcgcctga cgcaaaccga aattgatgtc aaacgtaccg tccaaatgcg ggtcgttaat
     3241 caaacctttc caacccaccg tcgtcctcgg cttctcgaaa taaacgcgca tcacaatcaa
     3301 aagctcgttt tcatactgct tgcggagttt caacaaacgc tccgcatatt ccaacgccgc
     3361 tttcggatcg tgaatcgaac acggcccgat aataaccaac agccgcttgt ccctgccgtg
     3421 aaccaaatcg gaaatttcct gacgggtgcg gtgaaccaag cccgaagcct ctttggaaat
     3481 cggcagctcg taaagatggg caatcggcgg caacaactct ttaacttctt taatcttaat
     3541 atcgtctgtg gggtaatggt gtgtcatctt gcatctttcc tgatggtttt ttatacgcgg
     3601 cttaggttac atcgtttacc ttgcacaaac aagtgcaaca tattactaca aagctatttt
     3661 atttcaaaat aactgatata tcttttttca tagattttaa atttcatatt tttaatattt
     3721 taggaatttt attccaaaga aaaagaaaca gattgataaa aacaataccc gccattcatt
     3781 cggacaaaac cgcacggaac atccgttttc ccatttccac gacaatccgg tacagaacac
     3841 cccgctgccg attccgcctg cacatccggc aaaatttcat acgcaaggaa aatgttgaaa
     3901 acaaaaagaa ctaattgctt gaaaaaagat ttaattggaa tgcgggtatt tcccacactt
     3961 tctccattta ttcatcctca ttataggaag aaaaacatga aaaaaccctg cttgcactct
     4021 cacttgcagc cgtgtccgcc gttacggtag ccgatattcc tgagtctaac ggcaagggca
     4081 aagccatcat cggcgataag gaaaaacacc gttaccaagc atgaaaacct tgttttgaaa
     4141 gtctatggcg aaatccgcca agatttgggg gacggatgat ccctgacccc ttgggcggat
     4201 gcatatgcag atttgcgcgg caaaaccaaa gtgatgacga cccagatgtg tgcttcccgc
     4261 gatgtcagca aaagcgccaa aggttggagt gtcggtatcg gtctgaatgt aggcaaacag
     4321 ttgaccgaca gcgtcggtct cgagtttgat ccatactacc gtcacaaaac aatctgcaaa
     4381 ccccgtgaga ttgttttgga cggcgacaaa accaaaatgg gccgctccaa atccaacgag
     4441 tacggcttcc gcgtaaccgc aacgttctat agtcaattaa aatcaaa
//
LOCUS       DAWFAN010000150         4476 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWFAN010000150 DAWFAN010000000
VERSION     DAWFAN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.150"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(505..611)
                     /locus_tag="WGO65_001715"
                     /pseudo
     CDS             complement(505..611)
                     /locus_tag="WGO65_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1030..1800)
                     /gene="xth"
                     /locus_tag="WGO65_001716"
     CDS             complement(1030..1800)
                     /gene="xth"
                     /locus_tag="WGO65_001716"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0327978.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPALPDDPQRRVIAATVSGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1860..2141)
                     /locus_tag="WGO65_001717"
     CDS             complement(1860..2141)
                     /locus_tag="WGO65_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0327979.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSNHLNRLRVEGLVDFTRYHRIIEYRLVSEEAAAILHTVRDLENKRVA"
     gene            complement(2548..3429)
                     /gene="nadC"
                     /locus_tag="WGO65_001718"
     CDS             complement(2548..3429)
                     /gene="nadC"
                     /locus_tag="WGO65_001718"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ0327980.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLACLAFQTMDPSVRFQAEIQDGQAVRAGQTLAVVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHLHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(3505..4443)
                     /locus_tag="WGO65_001719"
     CDS             complement(3505..4443)
                     /locus_tag="WGO65_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327981.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT         1037 a         1106 c         1192 g         1141 t
ORIGIN      
        1 cgtttcatga aactgatgca gatatagtgg attaacaaaa atcaggacaa ggcgacgaag
       61 ccgcagacag tacagatagt acggcaacgc tgtactggtt taaatttaat ccactatatt
      121 ttgtaatagc tgccattatg cctactagga tatatacctt ctatataatc cttatttaaa
      181 taaaaaccct tataaaaatc tgtgttatct gtcgggattg aaaaaaagat tttatctttc
      241 tcatcaggtt tatatttgct atcaatatca aaaagagaat aaggattaca atgagttcga
      301 catactaggt aaaaatttat atcttggatc atgaaatatt cgatatttat caggatcgaa
      361 agatgctaaa aattcatcaa tattggtact ggatattttg tcatcactaa taaacatcgc
      421 tagagatggg cgactttgat tttccatttg ttgattagta aaggatataa atactaactt
      481 gccattgtca aaccacatag atttatatag tggattaaat ttaaaccagt acgtcgttgc
      541 ctcgccttgc cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt
      601 aatccactat agttattgcc tttgggagaa tcaattctga cagcacataa catagattta
      661 tctttacagt cgttatattg agtttcatca aactcaactg catggcacaa taaaggcaat
      721 aggaaaaaca gggttattaa tttattacta tatataagac ggctttctat ttattcgaaa
      781 ataaatctag ttttcaatat ttgattttgt tttggattag tgtacataat aaattttact
      841 gctccaaaat ctgatattaa ccctatgtat ttcataaatt tttcaggaaa ataggctgtg
      901 tttgaacaat ccgccgattt cggctgtttg ccggggaaag gcaattattt ttcagtcaga
      961 tagaaagcta tcctattgaa taaattgaag ataaggatgc ttgtcaacgc catatcaaaa
     1021 cacggtcttt taccaatcga attctgccgt caccggcgcg tggtcgctcg gacgctccag
     1081 cgcgcgcgtc tccaaatcga cgcggacatc cttcaacgcc gccgccatcg caggcgacac
     1141 caaaatatgg tcgatacgca ggcccagttt gcgttggaac atcgcgccgc gatagtcgaa
     1201 ccaggtatag aacgcgcctt cgggatggac ttggcgcagg ctgtcggtca gtcccaaatc
     1261 cagcaggttt tgaaaccact gccgttcgac ggacgaacag tggatttttt cgtgccattt
     1321 ttcagggtcg taacagtccg catcggcagg cgcgatattg aaatcgccca gcaacaccag
     1381 tttgccgtgg cgggtcattt catcgcggac aaactccgtc agtgcggcaa accactgttc
     1441 cttatatttg aatttggggc tgtccaaagc ctcgccgttg acgcaataga cattgatgac
     1501 gcgcacgccg ctgacggttg ccgcaatcac gcgccgttgc ggatcgtccg gcagtgcggg
     1561 caaaccgaaa tgcacgtcct gcggcacgct gcggctgacg attgccacgc cgttgtaggt
     1621 tttctgcccg ctccaaacac agtgccagcc catcatttgc aaagcggcgg ccggaaattt
     1681 gtcctgatcg agtttgagtt cctgcaaaac caaaatatcg ggcggattgt cggcaagcag
     1741 gttttgcacc tgcggcagcc gcacattgag cgaattgaca ttccaagtgg tgattttcat
     1801 aacatttccg aaaaatgctg tctgaaacgt tcagacggca aaaggaaagg attctaacac
     1861 tatgccacgc gtttgttttc caaatcgcga accgtgtgca gaatcgccgc cgcttcttcg
     1921 gaaaccaggc ggtattcgat aatgcggtgg taacgcgtaa aatcgactag accttccacg
     1981 cgcaggcggt tcaaatggtt ggaaactgcg gtggccggca gggataagga ttttgccagt
     2041 tcggcgatat tgcgttcgct gtccaacaat tgaatcagta tcgccatacg ttcgggatgc
     2101 gccataagtt tgagtatggt gtggagcggt attgtgttca tcggtattcc taaaaccttt
     2161 gccgcccgcc aagcggattg acgggcggca gatatggatg attgtgatta tctttataaa
     2221 tcgtattata aatcaaatat ttatggatga ttgtacagga ttgtatgttc cttttcccac
     2281 tttttttgca aacaagcgta aaggcgggct ttcagacggc ataaaactgc cgccccccgt
     2341 cctttttcct gccctaccga tgccgccgcg ctgtcgggca aacaaaatac ctgcctgaaa
     2401 tgctgttttt tctaaagcat cacgaccttt acgaaaaatg ccgtctgaag aatctttcag
     2461 acggcatttc ctccctgagg caataaccgg attagggtta agcggttcct tgcctgacat
     2521 atcagacagc cgcccgcacc ctaaaactca cgccacgaaa tccaaaccta tgtccaacga
     2581 acggctgctg tgggtcagat agccgagggc gatgccgtcc actccggttt gcgccacgcg
     2641 cttcaggcgg tcgaagccga tgccgcccga tgcttcgcaa tagatggtgt ggaggtgggc
     2701 ggtttgcgtg tggcagcggt ttgccgcttc tttcagggtt tcgtcgtcca tgttatccag
     2761 caaaatccgt tccgcgcccg ctgcgatggc ttcgtccagt tgtgccaacg tatccacttc
     2821 gatttccacg caggtcaatg ctccgacagc ctgttttgcc tgctgcacgg cttgggcgat
     2881 gctgccgcaa taggcgaggt ggttgtcttt gatgagcacg gcgtcgtcca agcccatgcg
     2941 gtggttcacg ccgccgcctg ccctgacggc gtatttttgc aggacgcgca gcagggggat
     3001 ggttttgcgg ctgcacacga tgtctgtacc gtattcggcg acttcggcaa cggcacgcgc
     3061 ggtggcggtg gcgatgccgc ttaagtgcgt gaggtagttg agtgcggtgc gttcggcggc
     3121 gagcagcgcg caggcgttgc cttcgacgac ggccagcgtc tgacctgcgc ggacggcttg
     3181 tccgtcttgg atttcggctt ggaaacggac ggacggatcc atcgtctgaa aggcgagaca
     3241 cgccaagtcc ataccggcga taacaccgtc ttcgcggctg acgaggaaga gtttggcggt
     3301 tttgtcgggg gcgataacgg cggcggacgt aatgtcgccg cgcctgccca agtcttcgct
     3361 caaggcttgt tcgactatgg ggcgcaacag ggtgtcgggc agggaaaaga gggtgttttc
     3421 agacggcatg aggtttcctt tgtcgaaaaa gattgatgtt gcaccgatgg ggcaaaactt
     3481 catttgatgc cgtctgaaaa cgggctaacg gctgcccagc ggcagtccga tgtccgaaat
     3541 cagcctgtcg ggcaggacgt cgtcgcggaa gcggcaaagc tgcgcgggac ggcctttgct
     3601 gcccgatacg ccggtatccg acggctcgat gaggttttgc tgctgaatct ggcggcggaa
     3661 gttttgcttg tgcagcaatc ttccgctgat ggcttcgaca ctgttttgca gttgcagcag
     3721 cgtgaattcg ggcggcatca gctcaaaaat cacggggcgg tatttgattt tggcgcgcag
     3781 gcgggacagg gcggtcgcca atacgcggcg gtggtcgtgg cgcatgggct gccctgtgag
     3841 cgcgaaatcg aagtttgcct gcggctcggc ggcttccact atcaggccgc tttcatacag
     3901 catttcatag cgttgcaaaa cgtattcttc cgaccagttt tccggttcga ccccccaaca
     3961 caaatgaatg cgcttgagcc gcatttgacg cacttcctcc gtgtccgccg agtttgccca
     4021 aatgcgcagt cggctgacga cggcgtcgcg ctgtccgccg tcggtgcgca agtcttccca
     4081 cgggaaatag ccgtagcagt cctgccattt cgcatcggga tgcaggatgc tgtcggctgc
     4141 ctcgcgcacc agccccaaat agctgacgta caagacgggc atgccgtgtt cgttgcggcg
     4201 gtgggtatcg acaaaggtgt aaagctgttc cacatagccc ataggctgcg aagtctgctt
     4261 ggcaacccac agcttcacgc ccgcctgcaa ggaattgcgc agaggggaga gcgggccgtt
     4321 gggcaggagt gtgccttggg cgacggttaa tacccgcagg ccgccgtcgg taacggcaat
     4381 caatacggga accagctcga cgatgctttg cggcggggct tcggcttcgg ggtaggcatc
     4441 catgctcggc tttctgcggg tgggaaaggc tttatt
//
LOCUS       DAWFAN010000151         4465 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWFAN010000151 DAWFAN010000000
VERSION     DAWFAN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.151"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(45..3077)
                     /locus_tag="WGO65_001720"
     CDS             complement(45..3077)
                     /locus_tag="WGO65_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ0327982.1"
                     /translation="MFWIVMIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPTGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAICPFEDVPSERPSCRVSDTEADE
                     GAFQSEETDAVYEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGSSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3242..3922
                     /locus_tag="WGO65_001721"
     CDS             3242..3922
                     /locus_tag="WGO65_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ0327983.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3951..4310
                     /locus_tag="WGO65_001722"
     CDS             3951..4310
                     /locus_tag="WGO65_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ0327984.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
BASE COUNT          922 a         1292 c         1138 g         1113 t
ORIGIN      
        1 gccgtctgaa acagtcttca gacggcattt ccatttgcaa aaaatcaagc attgtccaag
       61 gggacgagaa tcgtacggtt gccgttgtgt tccggtgcgg acacaatgcc ttccgcctcc
      121 atctggtcaa tcagacgcgc ggcgcggttg tagccgatac gcaaggcgcg ctgtacgccc
      181 gaaatactgg ctttgcgcgt tttcaggaca acggatacgg cttcatcata catcggatcg
      241 gtttcgccgt cgccgctgcg cccgatgccg ggcagctctt cgctgctgcc gccgctcaaa
      301 atatcgtcaa catagtccgg ctcgccaaac tgtttcaaat attcgaccac gcggtgcacc
      361 tcttcatccg aggcaaacgc gccgtgaacg cgctgcggat aggcagtacc cggcggcagg
      421 aacagcatat cgccctgacc gagcaggttt tccgcgccca tttggtcgag aatcgtgcgg
      481 ctgtcgattt tgctggacac ttggaacgcg atacgcgtcg ggatgttcgc cttaatcaga
      541 cccgtgatga catcgacgct ggggcgttgt gtggcaagga taagatggat gcctgccgcg
      601 cgggcttttt gggcgaggcg ggcaatcagt tcctcgattt tcttgcctgc cgtcatcatc
      661 aaatcggcaa actcatcgac cacgaccacg ataaacggca gtttttccaa aggttcggga
      721 tcgtcgggcg tgaggctgaa cggattgccg attttttctc cccttgctgc ggcttcggcg
      781 attttttgat tgaagcccgc aagattacgc acgcccataa agctcatcag gcggtagcgt
      841 ttttccattt cgttaacaca ccagttcagc gcgtttgccg ccagcttcat atcggtaacg
      901 acaggggcga gcaggtgcgg gatgccttcg taaatgctca attccagcat tttcggatcg
      961 atcataatca tacgcacgtc ttccggcgcg gctttgaaaa gcatagacag aatcatcgcg
     1021 ttgacaccca ccgatttgcc cgaaccggtc gtgccggcaa ccaacaaatg cggtgctttg
     1081 cccaagtcgg ttacgacggg ctgtccggtg atgtcctgac cgagcgcgag cgtcagcttg
     1141 gatttggatt cggcaaactc gggcgaattg aagatttcgc tcaggcgtat catttggcgt
     1201 ttcgggttcg gaagttccaa acccatacag gttttgccgg ggatggtttc gacaacgcgg
     1261 atggaagcca cgccgagcga acgcgccaag tctttttcca gatttagaac ggaattgccg
     1321 cgcacgccga catcgggttc gatttcataa cgcgtaatca cggggccgga ataagaatcg
     1381 acaaccttga ccttgacttt gaactccgcc aatttttctt cgatggtgat gctgttttcc
     1441 aacagttctt cttcggtttg cgtcgcctcg ggattgaaca gcggcggcag aagcaggtcg
     1501 gttgtcggca ggtgttcgta taccgcatcg gtttcttcag attggaacgc cccttcatcc
     1561 gcttccgtat ccgatacccg gcaggacggg cgttcagacg gcacatcttc aaacggacaa
     1621 atcgcctgac tgtcatgccc gaacgcttcg gtttccgaca gatattgccc gtttgaccgc
     1681 tctgccgcac cttcactgcc gtcatctgca atagcggcgg tttcctcctg ccaacctcca
     1741 ttcaaaacat catcggcaag atggtcggtt tcagcaatgc ggctgcgttg cgcctgctcg
     1801 aatcctgtcg gcggctcata ggtacggttg tagatttccg atacgggagg cggcggcgga
     1861 atatcgattg cgggcatggg aactttcggc acttcgggtg gttcgaccac ccaagcatcg
     1921 ggcgcagcag gttcttcaat atagatatcg gcagcttcat cataaaccgg accgccgtat
     1981 cccacagacg aaacggtttc cgtgaaaaca ttttcggttt cagacggcat ttcgacatcc
     2041 cgtttcccga caaccgtccg actttccgga atcaggcgcg aaatccgcgc ctccgcagaa
     2101 accgttgcct tgttcggttc tttcaaattg acggaaacgc ggcggcaggc atcgcggacg
     2161 gctgtgccgt ctgaaacgga ctgtccttgg gaaacatccg gggattttgc ctccgcctgc
     2221 cctgcatgac ggcggaacgg cgcattccgt gcattttcgg ctttgactgc agaaaaggca
     2281 gacggatgct gcttctcaaa atacggctca aatccgtaat ccgcagactc ggaaaacgct
     2341 tctttgtccg catcgaacat acgggaatac gtgcgttgaa gcacagggtc gtccaaaccg
     2401 ataatctgta gcccttccat cggggtatcg gacacgcgga ctttggggac cgctgtttcg
     2461 tttttctcaa atgcatcgat atagcgtttt ttcgtttccc ttaaagccgc gctgtttaaa
     2521 gcacgcgttg cttcttccaa agtgatttcc ttaaaaaccg gacggacggg cgaaatttcg
     2581 ctttcagacg gcatcaaccc ttcagcaata ctccggtcga atgggatgcg gcggttgtcg
     2641 attacggcag ttgcaatgtc ttccgtatcg gcagcctctt cctccgcagc ttctgcttct
     2701 tccgtttccg cttcttccgt cccgtttcca ctgtctgaat atccgtcgga tgcggtcgga
     2761 acatccgcac ggtttgcaga agcaggcgga acgggcctgt ccggttcgga ttcgtgcgcc
     2821 agatagtttc ggcagaaacg gacgacaccg acaaacagcc aatacactgc cgtcttgacg
     2881 gcatggaaaa gcatcagggc aagttcggga aaatcgggca taccgtcttt gatttcaggc
     2941 agctccgcct gtttttcccc tttcttttcc tgccacgcag aaacctcgcg catccactcg
     3001 cgttcggatt gtgcgcggac aaaaaacaag ccggcaagcg caagcaacaa aataacgatc
     3061 ataactatcc aaaacatatg ccgtttcccg caagacaaac gattaaacgg gtttatttta
     3121 acgtgtttcc atcccaaaaa aaacaaccgg tcggcaattc tgccgaaacc gattcggctt
     3181 tcggtttgcc cccgccgaca gtataatagc cgtcctttcc cttaatcttt gcggcattgc
     3241 catgattttc caaacagtct ggttttcaga tatggtactg tcggtttcgt ggattgtcct
     3301 tatcctgata ctggcagctt ccgcgccgtc ggcattccgc tcgcttgccc gctaccgttc
     3361 cgcccttcca ctgtgtaccg taattttttc cgcagcatgg tgtctgaacg cctctgccgg
     3421 cggcggacag cttgcacaga tgaactacca cctgctcgcc gtcaatctgg ttgcactgat
     3481 ggtgggcact tccgccgcct tttggcttgc cgcgctgctg atgctgccct actgcctgct
     3541 gtttgccggt tctgccggcg catatccgcc gaacgcttta gtgctgattc tgcccgcgct
     3601 tgccgtcaac cgcctgtcgc gtatgctggt caaccgcctg ccgcccaata ttttcatctt
     3661 tattttcgtc aacggctttc tcgcttccgc cgccggcatt ctgttgaccg ggctggtgct
     3721 gaccggtatt ttggatgctg caaacgcttt tccgtccgaa atattgtgga cgaccgccct
     3781 gcccgtcttt attttgctgg cgtgggcgga agccttcctc agcggcattt caactgccat
     3841 atttgttgca ctgaagcccc attggattaa cactttcgac gacaaccgct atcttaagtc
     3901 cgaccgcggc atatggcggt agccttcaga cggcatagac aggcaaaacc atgctttcca
     3961 acatcatccc cctttccatc ggtgcggcac tcggtgcaac ggcgcgttgg ctgctcaacc
     4021 tcgccgttcc cgcatcgttg tctcccgcca cgggcaacct gtttgccaac tggacgggcg
     4081 cgctgctcat cggcatcttt gccgaaaccg tcaaccatcc gcaatggaag ctgctgctga
     4141 ttaccggttt tctcggcagc ctgaccacgc tttccggatt ttcactggaa accgtaaccc
     4201 tgctccaatc gaatcgtcct gcttcagcac ttgccaatat tttcctgcac acggcaggtt
     4261 cgctgctgct gacttggctc gggctgaaga tagggacggc agtcaaataa ccgcaacaaa
     4321 ttgcctccct ttgttttgac ataatctata aattagcttc gcttgtttaa atactggctg
     4381 tgccaattat agtagattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
     4441 atagtacgga accgattcac ttggt
//
LOCUS       DAWFAN010000152         4463 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWFAN010000152 DAWFAN010000000
VERSION     DAWFAN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.152"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WGO65_001723"
     CDS             complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WGO65_001723"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ0327985.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACTRLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="WGO65_001724"
     CDS             complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="WGO65_001724"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ0327986.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1964..3205
                     /locus_tag="WGO65_001725"
     CDS             1964..3205
                     /locus_tag="WGO65_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ0327987.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGDVSLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWAAGGLIADAKPSVLFDTQAPAGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSISGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3355..4410
                     /locus_tag="WGO65_001726"
     CDS             3355..4410
                     /locus_tag="WGO65_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0327988.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HSIPEYALIEPHLVDQHRTLKKLRSFLFSSRLTQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMRRELVGLYGVPPERIQ
                     VAPPPADTERFFPQPGETADLRAKYGFADYETVFLFPSTGHTRKGLELLADFFEHTGL
                     PVKLAVVGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESVLCGT
                     RVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYN
                     PSLSHHIDRLTDMLASV"
BASE COUNT         1047 a         1448 c         1029 g          939 t
ORIGIN      
        1 gtcctgattt ttgttaatcc actataaaat ggaaaatacc cggctatcgt ctcattttcg
       61 ttttaatcag ccataaaaat gccgtctgaa accctttcag acggcatttc tgtcaaacgc
      121 cggacgcact caatccaaac tcaacagcag gttgcggaac gcgttcgggt ctttgataaa
      181 cgtcatctcg cccgcctgcg gaaaatgaaa gtccaacagg cgcgacaccc aaaaacggat
      241 gcagccggca cgttgggcgg tcgggaaata cgccttttct tcggcactca aggggcgcac
      301 gccctcataa ccaccgataa acgccttttt caacgcctca tccaacttat tgtccgctgt
      361 ccttgcccaa tcgttgaccg caatcgccaa gtcatacata aaattgcccc ggcaggcata
      421 atagaaatcg atgaaacccg atacctgacc gccgtcaagc aacacattgt ctttaaacaa
      481 atcggcgtgg atgatgcccg aaggcagatg attgccgaga ttgtccttca gcgcatcgat
      541 ttcggaacac agcagcgcgg catcgtcttg cgacaggacg ggcagcagcc gggtgcacgc
      601 ctccgtccac cacgcatcgt aacgcgggtt ttccatttcc aaagggaaat cggcggcggc
      661 aaggtgcatt ttggccagca tcgcaccggt atggaaacac tgctccgccg tcggcagcgc
      721 ggtatccgaa cctttcaggc aggcaaccaa acaagccggc ttgcccgcca aaacggaatc
      781 aagccggccg tctttgcgcg caaccggcgc ggcaaccgcc acgcccttca tactcaaatg
      841 ccggttaagc tccagaaaaa acggcagctc ttcctgtttc aacacttcaa acacggtcag
      901 cacataacgt cccgaagtcg tcgtcagaaa ataattgctg ttggtaatcc cctgcgcgat
      961 gccctgcagg gaaacaaatt cccccaaatc gtaaccgctc aggaagccgc gcatttcatc
     1021 atcggaaaca ctggtataga cagacatatc aactcacttg ctcaaaaacg ggcaaacccg
     1081 ccgtcagaac gccggacggc aggcaaacat ataattcaca tcggtcgaat cgcacagggc
     1141 ataagtttgc gacaacacat ggtaagtcat acccttcgta tccgccacat ccaagcctgc
     1201 ctgacggcac attcgcgcca gctcggcagg tgcgatgaat tttttccagt cgtgcgtacc
     1261 tttggggaca aacttcaaca gatattccgc cgccacaatc agatgcaggt acgatttcgg
     1321 gtttttattg atggtagaaa aaaacaccat gccgtccggt ttgaccagat tggcacaagc
     1381 acgcacgatg gcggcgggat cggggacgtg ttccatcatt tccatgcacg ttaccacatc
     1441 gaacgagtgc ggttccgcct cggcaaggtc ttccacgcgg atacattcgt attcgatatc
     1501 ggcgacattg ttcaaagccg cgtgcaggcg ggcggtttcc aacgactgct ccgccatgtc
     1561 gatgcccttt acaaacgccg cgccgcgccg cgccatactt tccgccaaga tgccgccgcc
     1621 gcagcccacg tccaaaaccc gtttgccgca caaatccgcg tgtccgtcga tataatccag
     1681 ccgcagcgga ttgatgtcgt gcaaggtttt gaactcgccc gacttgtccc accatttgtc
     1741 ggcaatccgg ctgaatttgg cgatttcccc ctcatcgaca ttatattttt tgtcggacat
     1801 tttccctccc atctgacgaa ccgcccactc caaaacccaa gattatacta tggaacacaa
     1861 tgccgtctga aagccttttc gggcggcgcg gcgcaaatac cttacaaatc cttacacttt
     1921 acggcataat ggcggctcgc tttttctggc agaaagacaa aatatgccca acaaaacccc
     1981 ttcactgttc ggcggcgcga tgattatcgc cggcacggtc atcggcgcag gcatgctcgc
     2041 caacccgacc gccacatccg gcgtatggtt taccggctcg ctggccgtgt tgctgtacac
     2101 ctggttttct atgctttcca gcggcctgat gattttggaa gtcaacaccc attatccgca
     2161 cggcgcaagt ttcgacacga tggtcaaaga cctgctcgga cgcggctgga acatcatcaa
     2221 cggcatcgcc gtcgccttcg ttttatacct gcttacttac gcttatatct tcgtcggcgg
     2281 cgacctgacc gccaaaggct taggcagcgc ggcaggcggc gacgtttcac tcaccgtcgg
     2341 acaactcgtc ttcttcggca tcctcgcctt ttgcgtatgg gcatccgcac gcttggtcga
     2401 ccgcttcacc ggcgtcctta tcggcggcat ggtattgacc tttatttggg cggccggcgg
     2461 gctgattgcc gatgccaagc cgtccgtcct cttcgatacc caagcccccg ccggcacaaa
     2521 ctactggatt tacgccgcca ccgccctgcc cgtctgcctc gcttccttcg gcttccacgg
     2581 caacgtctcc agcctgctca aatactttaa aggcgacgcg cccaaagtgg ctaaatccat
     2641 ctggacgggc acactgattg cgctggtaat ttacgtcctc tggcaaaccg ccatccaagg
     2701 caacctgccg cgcaacgagt tcgcccccgt catcgccgcc gaagggcaag tctccgtcct
     2761 catcgaaacc ctgtccaaat tcgcccaaac cggcaatatg gacaaaatat tgtccctgtt
     2821 ttcctatatg gcgatcgcca cctcgttttt aggcgtaacg ctcggactct tcgactacat
     2881 cgccgacatc ttcaaatgga acgacagcat ctccggccgc accaaaaccg ccgcgctgac
     2941 cttcctgccg cccctgattt cctgcctgct cttccccacc ggcttcgtta ccgccatcgg
     3001 ctacgtcggc ctggcggcaa ccgtctggac aggcatcatc cccgccatgc tgctctaccg
     3061 ttcgcgcaaa aaattcggcg caggcaaaac ctataaagtt tacggcggct tgtggctgat
     3121 ggtttgggtc ttccttttcg gcatcgtcaa catcgccgca caggtattga gccaaatgga
     3181 actcgtcccc gtatttaaag gataaaggca aaatgccgtc tgaagcccgc cggcggcttc
     3241 agacggcatt gccgcaacaa acggcaaccg tattccggca cacagcgcat taccctgccc
     3301 ctcacgcaca aatcccgccc cgacaaaccg ggacgcaacc ataaaggaac aatgatgaag
     3361 ctcaaaatag acattgcaac caacaacttc aaacacggcg gcggcacgga acgctacaca
     3421 ttggatttgg taaagggtct gaacagacaa aacatcacac cggccgttta tgcgacgaaa
     3481 tttgatcaca gcattcctga atacgcccta atcgaacccc atcttgtcga tcaacaccgg
     3541 acgctgaaaa aactacgctc attcctcttt tcaagccggc tcactcaaac cagaaaaaac
     3601 agtgccgcca aactgattgc ctgccaccac gccgattacg ccgacctcct catctgcggc
     3661 ggcacacact tgggctacct gcaccatatg gcgcaaaaac cgaacctgct cgaccgcctc
     3721 gccatacgcc gtaaccgcag caactacgcc accgccaaac tgattatggc gcattcccat
     3781 atgatgcggc gcgaactggt cggactgtac ggcgttcccc ctgaaagaat ccaagtcgcc
     3841 cccccccccg cagatacgga acgcttcttc ccgcaacccg gagaaactgc cgacctgcgc
     3901 gccaaatacg gctttgccga ctatgaaacc gttttcctgt tcccatcgac cggccacacg
     3961 cgcaaaggtc tggaactgct tgccgacttt ttcgaacata ccggcctgcc cgtcaagctc
     4021 gccgttgtcg gctccccgct cccccgccct atgaaaaacg tcgtcgggct gggcttctgc
     4081 accgatatgc ccgaactcta ccgcgccgcc gactttacca ttatggcctc cctgtacgaa
     4141 cccttcgggc tggtcggtgt cgaatccgtc ctatgcggca cacgcgtcgt cctctccgaa
     4201 aacatggcat gtacagaggt catgaacgaa gaagccggat tctttttctc acgccaaaac
     4261 ccggaaaccc tggcgcaagc cgttgcccaa gccgtcagcc ttaaaaaaca gggcggacac
     4321 cgcctgtccg acccgatgcg tgcactgaac tacaacccgt ccctgtcgca ccacatcgac
     4381 cgactgaccg acatgctggc atccgtataa caaaatgccg tctgaacgag ttttcagacg
     4441 gcattgctgc gttatagtgg att
//
LOCUS       DAWFAN010000153         4423 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWFAN010000153 DAWFAN010000000
VERSION     DAWFAN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.153"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(26..349)
                     /locus_tag="WGO65_001727"
     CDS             complement(26..349)
                     /locus_tag="WGO65_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0327989.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(337..576)
                     /locus_tag="WGO65_001728"
     CDS             complement(337..576)
                     /locus_tag="WGO65_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ0327990.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(699..1496)
                     /gene="truA"
                     /locus_tag="WGO65_001729"
     CDS             complement(699..1496)
                     /gene="truA"
                     /locus_tag="WGO65_001729"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ0327991.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALENALAQI
                     AGEAVSTIVAGRTDTGVHATAQVVHFDTTAARPQQAWIRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
     gene            complement(2292..2984)
                     /locus_tag="WGO65_001730"
     CDS             complement(2292..2984)
                     /locus_tag="WGO65_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0327992.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAPSSLTSAKTFWGV
                     CRKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            complement(2981..3724)
                     /locus_tag="WGO65_001731"
     CDS             complement(2981..3724)
                     /locus_tag="WGO65_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0327993.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            complement(3753..4316)
                     /gene="gmhB"
                     /locus_tag="WGO65_001732"
     CDS             complement(3753..4316)
                     /gene="gmhB"
                     /locus_tag="WGO65_001732"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ0327994.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
BASE COUNT          869 a         1054 c         1409 g         1091 t
ORIGIN      
        1 aacacaatcc attcagacta ttcaatcagg caaacatctc ctgcaatact gcaaacagtt
       61 tttcagccgt actgttgtct aaatttccaa gatgtttgac caatccggct ttatccacag
      121 ccctaatctg ttcgggcaaa agcaaaccgt ctttatcctg aaagcggaca ttgacgcgga
      181 acggggcagg acggcttccg ctcgtcatgg gaacgatcag cacagtcttg agatagttgt
      241 gtatttcagg aggagagact acgacacaag gacgtgtctt tttgatttcg cttcctacgg
      301 tcgggtctaa ggagaccaga tagattccgc cgcgtactac catatccatt ctttatccgc
      361 ttcgttttca atttcggaaa aaaaatgctc ctgctcggtt tcgacaagca ttgcggcagc
      421 ttctgcccat cccctgcgaa cggtaggaca gcttaaaatg atattgccct tttcaactgt
      481 aacagccaag ctgtctactg cccctatttg acccaataat gatttgggca gaatcacgcc
      541 ttgcgagttt cccattttgc gtatgttgag aatcatatac gtaccatatt caccagttta
      601 tgtaataaca atgttagtac cttgatgagg tagtgtcaac attgaaaaag attgcctgac
      661 agtttgtccg atttcaaaat ctccgcgaca agaatgtttt aaagccattc ggggatttgg
      721 gggcggatga tgccgtatgc ctcgggatag tcgacgccgg tcaggtaaag cccgtcgggc
      781 ataaaggtcg gtggggcttt gaggcgactg cgttcttgaa tcagtgcggc gaagccttcg
      841 acgctgagtc tgccgctgcc gacatagacg agcgcgccca tgatgttgcg taccatgtgg
      901 tgcaaaaagg cgttgccgtg caaatcgagg cggacgagtc ctgcgctttg ggtaaggtcg
      961 gcgcggtaga tggttttgac gggggatttt gcttggcatt cggcggcgcg gaagctggag
     1021 aagtcttgtt cgccgatcaa ataggcggca gcccgacgca tcggttcgat gtcgagtttg
     1081 aggtgtgtcc agcctgccct gtttttgagc aggggggaac ggacgggggc ggattcgagc
     1141 aggtagcggt agtgccgtcc gtatgcgtca aatcgtgcat gaaattcggg ggcgacctgt
     1201 cgggcgtgca aaacggcaat gccttcgggc aggtgggcat ttacgccgcg tatccatgcc
     1261 tgttggggac gggcggcagt tgtgtcgaag tggacgactt gggcggtggc atgcacgccg
     1321 gtgtcggtcc tgccggcaac aatggtggaa accgcttccc ctgctatttg ggcaagtgcg
     1381 ttttccaatg ccgcctgaac ggtcggtacg ccgtcagcct gtttctgcca gccgtaaaag
     1441 cggctgccgt cataggatag ggttattgcc cagcgttgtt tttgtgcggt atccatcgga
     1501 tttgggattc ggataaatgt tcagactgca ttgtatcgca gattttgcag ggaaacggca
     1561 aacgcccagg gcgagcggcg ttgtttgggg agttgggggg tggatgcagt tgctacgaat
     1621 cgctatcctg tgaattcacc ctgtcaggag tgcccgaatc gtcattcccg cgcaggcggg
     1681 aatctaggtc tgtcggtgcg gaaacttatc gggtaaaacg gtttcttcag attttacgtt
     1741 ctggattccc actttcgtgg gaatgacggg atgtaggttc gtgggaatga cgcggtgcag
     1801 gttttcttaa ccccgcgttc tagattccca ctttcgtagg aatgacggcg gtagatttgg
     1861 cagatgcggc ggattcggca ggtctcaacc catcctacaa tccaccctga ccgcctttta
     1921 cgaatccgcc gccatcctgg gaaacgcaaa aaaatgccgt ccgaaaacct ttcagacggc
     1981 attttcgcgg gcaaatcagt aaaagacttc gcgtcagctt aagcgtttca taccgcacgt
     2041 cggaccgggc gggtttcggg tttcggaaga gtggtgtgaa atgaaacgcg gcagacggcg
     2101 tgtgtgccgc catcctgccc cgaaccggcg gcaaagcgtc atcgcggttt gaatcgggcg
     2161 ggcgcgtgcc tgttccggca cggatgccgt ctgaaagcgg cggcagcggt atgtgtcgcc
     2221 ggctgtgatg ctgctttccg gatgtccgtt tgggaggagg tctgaattgg cggatgcctg
     2281 cccgggccgc gtcagcctaa ggcgaaaagt tccctgccgt ggattttgag ccactgtttc
     2341 gcttcgggcg tgtagggtgc gaagcggcgg gtcagtgccc agaacgcgcg gctgtggtcg
     2401 gcgtgggcga ggtggcagag ttcgtgtatg cagacataat cggcaacgta ttccggtgcg
     2461 ccgaccagcc gccggttgag gcgtatgcct gtggttttgc ggcacacgcc ccagaaggtt
     2521 ttggcagagg tcagcgagga gggggcgggg aacagttgtg tggtgcgggc gtggcgttcg
     2581 aggcggggaa tcaggtaact gtgcgcctgc cgttccaaaa agtcccgcag cagcgcaagc
     2641 tgtttttcgg gtgcgccttc gggaacacgg atttcagacg gcatcagcag gatttgcgtg
     2701 tcttgatggg cggtgagggc aagctgtctg ccgtggaaga ggatggattc gggcagccgg
     2761 ttttcggcag tttgcggcgg cggtgttttc gccagtgttt gccgcaggac ggcttcgttt
     2821 tcatacagcc agcggtttag agcggagacg gagaagcagg gtgggacgct gatgcggacg
     2881 gtatgtgtgc cggcggggcg gataatcagg tttttcttgg cacggcgctt gatttcgacg
     2941 gtcagttcca tgccgtctga aagggtgtgg acaaaggcgg tcatgcggtt tcagacggca
     3001 ttttggcggc aaacgggcct gcgccggaaa tgagcggctg ctgcgtttcg atgaggtgtt
     3061 cgcattttcc catcaattcg gcttcgctgc cgcttgcgtg cgggatggtc ggacagatga
     3121 cgacggtgat ttcccccgga tatttcagaa aggagttttt cggccaaaat tcgccgctgt
     3181 tgagggcgac ggggacgatg tccatctcaa acattttcgc catgcgcgcg ccgccgagtt
     3241 tgtatttgcc gcgttttccg ggcgcaaggc gcgtgccttc ggggaaaatg gtaatccaat
     3301 agccttcgtt tttgcgcgcc aacccctgtt ttatgagctg ctcgttggct tcgcggcggt
     3361 tgttgcggtc tatgcctatg gttttgacca gtttcaagcc ccagccgaaa aaggggattt
     3421 tgaacaactc gcgtttggca acgtaaacct gcggcggaaa aatgtcctga agggcgagcg
     3481 tttcccagcc gctttggtgt ttggcgcaga tgacggcggg gcggtcgggg atgttttccg
     3541 cgccgatgat gcggtatttg agcccgacga tgtgtttgag cgagaggttg aggattttga
     3601 cccagacccg cgccatcttg tgcgccccgt ctcggaaagg cgaggcgagc agcataaagg
     3661 gaaagaggaa aatcaggctg gaacagagta tcagccagta aatcaggttg cggatgatga
     3721 gcatggtttt gccttgtcgg aatgcggtat gttcagtcgg cttgcggtgc ggtgttttcc
     3781 tgcatgatgt attgtgagaa atcgagcagg gtatcgaaaa cctgtgtgtg ttcgggtaat
     3841 tcgtgtccgt gttgggagag cgtttttttg ccttttccgg tcagaaccag cgcgggtttt
     3901 ccgccgacgg catcgattgc ctgcaaatcg cgcaggctgt cgccgaccag ccaggtttcc
     3961 gaggcttggg cgttgaagcg tccgatgatg tcttcaatca tacccggttt gggcttgcgg
     4021 cagttgcagt tgtcggcatc ggtgtgcggg cagaaccaga tgccgttgat ttcgccgcct
     4081 gcctgacgga cgaggcggtg cattttggcg tgcatttcgg taaggttttg aacggtaaaa
     4141 tatttgcgcc cgatgccgga ttggttggtg gcaacggcga cggtgtagcc tgcctgcgtc
     4201 agaaatgcca ccgcatccat gctgccttcg acaggtatcc actcgtcaac ggatttgacg
     4261 aagtcgtcgc ggtcctgatt gatgacgccg tcgcggtcga gaatgatgag tttcatcgcg
     4321 tttcctttgg gttggggcag gtcgggggtg gcattatact gaaatatcgg cagtgatgcg
     4381 cctgtgccgc gtgatatagt ggattaacaa aaaccagtac ggt
//
LOCUS       DAWFAN010000154         4416 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWFAN010000154 DAWFAN010000000
VERSION     DAWFAN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.154"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            185..2032
                     /locus_tag="WGO65_001733"
     CDS             185..2032
                     /locus_tag="WGO65_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0327995.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETAGLSAS
                     VPTRPAEPEGKTLADYSSYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAAMGNTQEAEKLY
                     KQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAVKRALVLFQNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREIA
                     GKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADIPI
                     EGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGTVPAR"
     gene            2321..2533
                     /locus_tag="WGO65_001734"
     CDS             2321..2533
                     /locus_tag="WGO65_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ0327996.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2665..3111
                     /locus_tag="WGO65_001735"
     CDS             2665..3111
                     /locus_tag="WGO65_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ0327997.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            3132..3281
                     /locus_tag="WGO65_001736"
     CDS             3132..3281
                     /locus_tag="WGO65_001736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0327998.1"
                     /translation="MPSEARIAGSDGIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(3310..3702)
                     /locus_tag="WGO65_001737"
     CDS             complement(3310..3702)
                     /locus_tag="WGO65_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ0327999.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFSGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3773..4378)
                     /locus_tag="WGO65_001738"
     CDS             complement(3773..4378)
                     /locus_tag="WGO65_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0328000.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
BASE COUNT         1085 a         1238 c         1220 g          873 t
ORIGIN      
        1 gtctgaaacg ggcaaaggca gcggttctca ccaaaacggc aaatgattga aaaatataaa
       61 ttatgtcaga aacattccaa agccggtgca gtttcggcat ataattcggg caaacgcctg
      121 ttcagacggc attttgtctt ttccaaccct gaccgttcag ggttccgatt cttaaggaaa
      181 tccgatgtac ctaccctcta tgaagcattc cctgccgctg ctggcggccc tggtgcttgc
      241 cgcgtgttct tcgacacacg cgcccagcag caagaccctg gcagacaata tagaaactgc
      301 cggtctttcg gcaagcgttc ccacccgccc tgccgaaccc gaaggaaaaa cgctggcaga
      361 ttacagcagc tacccgtccg cactggatgc agtgaaacag aaaaacgatg ccgccgtcgc
      421 cgcctatttg gaaaacgccg gcgacagcgc gatggcggaa aatgtccgca acgagtggct
      481 gaagtctttg ggcgcacgca gacagtggac gctgtttgca caggaatatg ccaaactcga
      541 accggcaggg cgcgcccaag aagtcgaatg ctacgccgat tcgagccgca acgactatac
      601 gcgtgccgcc gaactggtca aaaatacggg caaactgcct tcgggctgca ccaaactgtt
      661 ggaacaggca gccgcatccg gcttgttgga cggcaacgac gcctggaggc gcgtgcgcgg
      721 actgctggcc ggccgccaaa ccacagacgc acgcaacctt gccgccgcat tgggcagccc
      781 gtttgacggc ggtacacaag gttcgcgcga atatgccctg ttgaacgtca tcggcaaaga
      841 agcacgcaaa tcgccgaatg ccgccgccct gctgtccgaa atggaaagcg gtttaagcct
      901 cgaacaacgc agtttcgcgt ggggcgtatt ggggcattat cagtcgcaaa gcctcaatgt
      961 gcctgccgcc ttggactatt acggcaaggt tgccgaccgc cgccaactga ccgacgacca
     1021 aatcgagtgg tacgcccgcg ccgccttgcg cgcccgacgt tgggacgagc tggcttccgt
     1081 aatctcgcat atgcccgaaa aactgcaaaa aagcccgacc tggctctact ggctggcacg
     1141 cagtcgcgcc gcaatgggca acacgcaaga ggcggaaaaa ctttacaaac aagcggcagc
     1201 gacgggcagg aatttttatg cggtgctggc gggggaagag ttggggcgca aaatcgatac
     1261 gcgcaacaat gtgcccgatg ccggcaaaaa cagcgtcctc cgtatggcgg aagacggtgc
     1321 agtcaaacgc gcactggtat tgttccaaaa cagccgaacc gccggcgatg cgaaaatgcg
     1381 tcgtcaggct caggcggaat ggcgttttgc cacacgcggc tttgacgaag acaagctgct
     1441 gaccgccgcg caaaccgcgt tcgaccacgg tttttacgat atggcggtca acagcgcgga
     1501 acgcaccgac cgcaaactca actacacctt gcgctatatt tcgccgttta aagacacggt
     1561 aatccgccac gcgcaaaatg ttaatgtcga tccggcttgg gtttatgggc tgattcgtca
     1621 ggaaagccgc ttcgttatag gcgcgcaatc ccgcgtaggc gcgcaggggc tgatgcaggt
     1681 tatgcctgcc accgcgcgcg aaatcgctgg caaaatcggt atggatgccg cccagctcta
     1741 caccgccgac ggcaatatcc gtatggggac gtggtatatg gcggacacca aacgccgcct
     1801 gcaaaacaac gaagtcctcg ccaccgcagg ctataacgcc ggtccaggca gggcgcgccg
     1861 atggcaggcg gatatcccca ttgagggcgc ggtatatgcc gaaaccatcc cgttttccga
     1921 aacgcgagac tatgtcaaaa aagtgatgac caatgccgcc tactacgccg ccctcttcgg
     1981 cgcgccgcac atcccgctca aacagcgtat gggcaccgtt cccgcccgct gacgtaccga
     2041 tgccgtctga aacccgcccg gtcttttaga cggcattttt atcccgaacg gcattgacgg
     2101 cgaaccataa atataagaca atccgaaaat tgtttttcct gctttttcaa gcagcttgac
     2161 acggcacaag ccgacccgtt aggaggtgat gtttccgtca cggcgcgtat cccgccgccg
     2221 aaaggcacag cgatacggaa aacctacaac accgtctgcc ctaccctttc caccgatatg
     2281 atgggcagat gcaacaacag aatttattaa aggaaataaa atgcctgcaa tccgcgtaaa
     2341 agaaaacgaa ccctttgaag tcgctatgcg ccgtttcaaa cgcgccgtag aaaaaaccgg
     2401 cctgctgacc gagctgcgcg cccgcgaagc ctacgaaaaa ccgactaccg aacgcaaacg
     2461 caaaaaagcg gcagccgtaa aacgcctgca aaaacgcctg cgcagccaac agctgccgcc
     2521 caaaatgtac taaacgttca agtacagatt acaggtcagc cctgtgatat gaggacacac
     2581 cgcaagacct gctctgcggt gtgttttgct tttcagacgg catcgaaacc cgccgtctcc
     2641 atccgacatc ccagcgagga cattatgagc ctgaaaatcc gccttaccga agacatgaaa
     2701 accgcgatgc gcgccaaaga ccaagtttcc ctcggcacca tccgcctcat caacgccgcc
     2761 gtcaaacagt ttgaagtgga cgaacgcacc gaagccgacg atgccaaaat caccgccatc
     2821 ctgaccaaaa tggtcaaaca gcgcaaagac agcgcgaaaa tctacactga agccggccgt
     2881 caggatttgg cagacaaaga aaacgccgaa atcgaggtac tgcaccgcta cctgccgcaa
     2941 atgctctccg ccggcgaaat ccgtactgcg gtcgatgccg ccgttgccga aaccggcgcg
     3001 gcaggtatgg cggatatggg caaagtgatg ggcgtattga aaacccgcct cgccggcaaa
     3061 gccgatatgg gcgaagtcaa caaaattctg aaagccgtac tgaccgcctg attgtatgaa
     3121 tataaggaaa aatgccgtct gaagcccgta tcgcaggttc agacggcatt ttcaatgtcc
     3181 caatatcgaa tcggcagggg caacacggtt tcgatacacc gaaacgggtt ttgccgataa
     3241 acggattccg tttgcgcccc atcggacaaa atgccgtctg aaacacgatt ccgttcagac
     3301 ggcatagatt tatttgacca atttcaagcc ttttttggcg ggccggggcg cggtttcggc
     3361 agaggtgttt tcaggcggcg tgtcggggcg atacgcttcc aattcaaacc ccataccttc
     3421 gccggtctcc cgtgcgaaaa ggctgaggac gtgtccgaca ggtatccata tatcgtgcgc
     3481 ctgtccgctg aaacgggcgg aaaaactgat ccaatcgttg tcgatttgaa ggttttgcgt
     3541 ggcggtcgcg ccgatgttga gcataatttc gttgtcgcgg acatactgca tagggacgcg
     3601 cgtgtgttcg ttgacccaga caaggatgtg cggtgtgagg ctgttgtcgc tgcaccattc
     3661 gcagagggcg cggaggatgt agggtttggt ggaagtgggc ataatggttc cgtgttgtac
     3721 gccaaaatag gaaaatgcct gcaaaacggc gggttttgca agcatttcgg acttatttgc
     3781 gcatggcttt ttcggcgggt gtcagtgctt cgataaaggc ttcgcgctgg aagatgcgct
     3841 cggcgtattt gagcagcggc gcggcacttt tgcccagttt gacatcgtag tggtcgagcc
     3901 gccacagcag cggagcaagg gcgacatcaa tcatagaaaa atcttcgccg aggatgtatt
     3961 tgcttttgct gaacgaaggg gcgagcatgg tcagaccgtt gccgatggct tcgcgcgctt
     4021 ttgcctgctc cttattggta gcggcggggt tttccaacac ttggacgtgg ttgaacaatt
     4081 ctttttccat acggtacagc accagccggc ccctgccgcg cataacggga tcgccgggca
     4141 taagctgcgg atgagggaag cgttcgtcga tgtattcgtt gatgatgttg gactcgtgca
     4201 gcaccaaatc gcgctcgacc agtacgggga cctggttgta cgggttcatg acggcgaggt
     4261 cttcgggttt gttgtaaata tcgacgtctt tgatttcaaa atccatacct ttttcgtaca
     4321 aaacgaagcg gcagcggtgg ctgaaggggc aggtaatgcc ggaatagagg gtcatcataa
     4381 taattgtcgc tcctgtgtga tgcctacaaa acggct
//
LOCUS       DAWFAN010000155         4320 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWFAN010000155 DAWFAN010000000
VERSION     DAWFAN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.155"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..248)
                     /locus_tag="WGO65_001739"
     CDS             complement(<1..248)
                     /locus_tag="WGO65_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328001.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITR"
     gene            complement(245..391)
                     /locus_tag="WGO65_001740"
     CDS             complement(245..391)
                     /locus_tag="WGO65_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328002.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(408..746)
                     /gene="erpA"
                     /locus_tag="WGO65_001741"
     CDS             complement(408..746)
                     /gene="erpA"
                     /locus_tag="WGO65_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ0328003.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(878..1564)
                     /locus_tag="WGO65_001742"
     CDS             complement(878..1564)
                     /locus_tag="WGO65_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0328004.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFLDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            1752..2024
                     /locus_tag="WGO65_001743"
     CDS             1752..2024
                     /locus_tag="WGO65_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328005.1"
                     /translation="MKKTGKYLIYTAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2232..4160
                     /gene="dnaK"
                     /locus_tag="WGO65_001744"
     CDS             2232..4160
                     /gene="dnaK"
                     /locus_tag="WGO65_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ0328006.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         1325 a         1141 c          864 g          990 t
ORIGIN      
        1 ctccgggtaa taatatccaa acccaaatag ctgaaataat cgtgcaactc ttcccgtttc
       61 gcccaagtgc atttttcctc aaccagcact tgaggtttgg aatcgagcga ttcgatcaat
      121 ccttccgcct tttcctcgcc gagattctcc ctgaggcact cccggataag cccgcgatcc
      181 acttggggcg ggtaggaaac atatacatcg tacaaatcat ttcggtaatc tttactcatc
      241 tcaatcataa gggaacaaat acgcctgata ccgttccgcc cgttcctttc gggaaacccg
      301 attcatcttc aaacaatcgg attatatgtg aattcatccc gataatatat tttccaaaag
      361 taaaaatgcc gtctgaacgt tcagacggca tcacacaaac caagcggtca tacggaaaac
      421 gacgatccgc aaccgcaggt tgtttccgca ttcggattgc ggatgacgaa ttgcgaaccc
      481 tgcaaacttt ccgtatagtc gatttccgca ccgaccagat attgatagct catcggatcg
      541 accaaaaaga ccaaaccgtt tttctcaatt tcaaaatcgt cgtcgttttt gatttcgtca
      601 aaagtaaatc cgtactggaa acccgaacag ccgccgccat tgacaaaaac ccgcaatttc
      661 aaatcgggat tgttttcttc ggcaatcaaa tcggcaactt tggcacagca gctgtcagta
      721 aaaataatag ggctttcatc cgacatggtg ttattcctta atataatatt ttctgcattc
      781 tctctcaaac cccctttaaa ggcaagcagg cttcgccttt gaaaaaggaa ttgttccaat
      841 agataagttt gaccgcctgc aatttcaagc cccaaactca aatcgtcctg ccgaaccact
      901 cgacacgccc gataatctcc acatcatcgg tcaaatcgtt caaattgatt tcaaaagcag
      961 gataagcctc gtttgcagaa atcacattga taatcccgcc cggtacaatc tgtaaacgtt
     1021 taaccagcag attttcatta atccgcaaca catacagacc gtccctcggc gtattttcac
     1081 catgattgac caaaatcgaa tcgccgtcat tcaaaacccc ctccatcgaa tcccccttga
     1141 cggaaattac agacaggttt ttcgtatcgc gggtaacata attctcaatc caatgccgtc
     1201 tgaacgccat tgtaaatacc ggttcctcat gatcgacaaa ctgcccgtat cccgcagccg
     1261 cccgaatatc atatctcggc acaaagacaa actcgtccgt atcgacttca tttcccaagg
     1321 tatcgtaagc aagggatttt tttggggctt catccagaaa cggattaccc tccccggtca
     1381 gcagccaatc gatactacac cccttcaact gcttgatttt tttcaatgtt tcagactttg
     1441 gcagaccgcc ttcattccat atcctgctga agcccgcaat cgtcatttca atatcggatg
     1501 cgatttttgc ctgcctcgct tcgcttttcc ataaaaaaac cagtctgtct ttaaaggttt
     1561 ccataaatat ccttccactc cccttcattc aatcatctaa agtaaacatg aaatatattt
     1621 taccttagct atccaaaaaa cggaacaatt atgataaact aaatttaaat tatgattttt
     1681 cttacttaat acataatttt tcagattttt ccataggttt aatcctgaat tgacagaaaa
     1741 aggatagaaa aatgaagaaa accggcaaat atcttatcta tactgcggca tttaccgcat
     1801 tctgctttgc cttccaagaa aaccgttctg aagccaaaca gcccgacatc actttatccg
     1861 catccctgtg cgaacaattc aacatactga acgccaaaga tatggatgca gaacaagtct
     1921 ccctttccaa agaatgcgac atcatcgagt cttcacacga ctgggaaaaa gagtacggca
     1981 acttgaacga acaggaaatg ctcgccggca tcgtctacga ataaacctgc ctgccatttg
     2041 aaatattatg cttgaatgca ttggagccaa atgtattaaa tcaaatataa aaccaatata
     2101 ttcataaagt tatatactta tagccatgct gtagcttgaa acagcccgcc gcccgcccta
     2161 tttacagccc atcgggacaa aatgttccaa catatttctc aaaataagca aaatcaaata
     2221 ggattatcca catggcaaaa gtaatcggta tcgacttagg tacaaccaac tcttgtttgg
     2281 ccatttccga aaacggtcaa accaaagtta tcgaaaacgc agaaggcgca cgcaccacgc
     2341 cgtccgttat cgcttatttg gacggcggcg aaatcctcgt cggcgcgcct gccaaacgcc
     2401 aagcggtaac caacgccaaa aacactattt acgccgccaa acgtttgatc ggccacaaat
     2461 ttgaagacaa agaagtccaa cgcgacatcg aatctatgcc tttcgaaatc atcaaagcca
     2521 acaacggcga cgcatgggta aaagcacaag gcaaagagct gtctcctcct caaatttccg
     2581 cagaagtcct gcgtaaaatg aaagaagccg ccgaagctta cttgggcgaa aaagtaaccg
     2641 aagccgtgat taccgttccg gcctacttca acgacagcca acgtcaagcc accaaagacg
     2701 caggccgtat cgccggtttg gacgtaaaac gcatcatcaa cgagccgacc gcagccgctt
     2761 tggcattcgg tatggacaaa ggcgacaaca aagaccgcaa agtagccgta tatgacttgg
     2821 gcggcggtac tttcgatatt tccatcatcg aaatcgccaa cctcgacggc gacaaacaat
     2881 ttgaagtatt ggctaccaac ggcgatactt tcttgggcgg tgaagacttc gaccaacgct
     2941 tgatcgacta catcattgac gagtttaaaa aagaacaagg cattgatttg aaacaagacg
     3001 tcatggctct gcaacgtctg aaagaagctg ccgaaaaagc caaaatcgaa ttgtccagcg
     3061 gccagcaaac cgaaatcaac ctgccgtaca tcaccatgga cgcaaccggc ccgaaacact
     3121 tggcgatgaa aattacccgc gccaaattcg aaagcctggt tgaagacctg attgcccgct
     3181 ctatcgagcc ttgccgcacc gcattgaaag atgccggtct gagcactggc gacatcgacg
     3241 acgtgatttt ggtcggtggt caaagccgta tgccgaaagt acaagaagcc gttaaagact
     3301 tcttcggcaa agagccgcgc aaagacgtga accctgacga agctgtcgcc gtaggcgcag
     3361 cgattcaagg cgaagtgtta agcggcggcc gcagcgacgt attgctgctg gacgtaaccc
     3421 ctctgtcctt gggtatcgaa accatgggcg gcgtgatgac caagctgatt caaaagaaca
     3481 ccaccatccc gaccaaagcg tcgcaagtgt tctctaccgc cgaagacaac caaagcgcag
     3541 taaccatcca cgtactgcaa ggcgaacgcg aacgcgcttc tgccaacaaa tctttgggtc
     3601 agttcaactt gggcgacatc gcacccgcac cgcgcggtat gccgcaaatc gaagtcacct
     3661 tcgatattga cgccaacggt atcctgcacg tttccgccaa agacaaaggc actggcaaag
     3721 cagccaacat caccatccaa ggttcttcag gtttgagcga agaagaaatc gaacgcatgg
     3781 tgaaagatgc cgaagccaat gccgaggaag ataaaaaact gactgaattg gtcgcttccc
     3841 gcaaccaagc cgaagccctg attcactctg tgaaaaaatc tttggccgac tacggcgaca
     3901 aactcgacgc agccgagaaa gaaaaaatcg aagccgcgct gaaagaagcc gaagaagcag
     3961 ttaaaggcga cgacaaagcc gctatcgatg ccaaaaccga ggcgctgggc gcagccagcc
     4021 aaaaactggg ggaaatggtt tacgctcaag cacaagctga agcccaagca ggcgaaagcg
     4081 aacaagccaa tgcttctgca aagaaagacg atgatgtcgt agatgccgac tttgaagaag
     4141 taaaagacga caaaaaataa ttaataccgt ctgaaaaaag cgcgaaccgt ttgattcgcg
     4201 cttttttcaa ttgagataaa agaccatagc ataacagagg ttccaagttc atctaccgta
     4261 atattcccaa acccaagttc caactgttgt atcggttctc tggaaatttt ttatatagtg
//
LOCUS       DAWFAN010000156         4277 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWFAN010000156 DAWFAN010000000
VERSION     DAWFAN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.156"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(1036..1473)
                     /gene="rnhA"
                     /locus_tag="WGO65_001745"
     CDS             complement(1036..1473)
                     /gene="rnhA"
                     /locus_tag="WGO65_001745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ0328007.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1586..2455
                     /gene="tehB"
                     /locus_tag="WGO65_001746"
     CDS             1586..2455
                     /gene="tehB"
                     /locus_tag="WGO65_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ0328008.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEILLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGSAEVWSADGGDCVFAPQQVFSVKPKTDDAEIRLSMYCTAADYFH
                     KKYGMSATHSAVSAAQDTVPAGRALDMGCGQGRNALFLGLKGFEVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(2505..3311)
                     /gene="thiD"
                     /locus_tag="WGO65_001747"
     CDS             complement(2505..3311)
                     /gene="thiD"
                     /locus_tag="WGO65_001747"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ0328009.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
BASE COUNT          932 a         1058 c         1203 g         1084 t
ORIGIN      
        1 tgtaacccct tccaaatcag gacgacacat agggaggtgc tttatgtgtc gtcctgtgtg
       61 ttgaaatata tcggacgcgt ttgaacagtt gtgcggcaaa ctgttttagc ggcaggcaag
      121 agatgccgtc tgatgccgtc tgaagcccat tgtccgtcat tcccacacag gcagttgcta
      181 cggttgctga agccccttag ccgtcgcgca ggcggttgct acggttacta aaatcccgtc
      241 attcccgcgc aggcgggaat ccagtccgtt cggtttcagt tgtttctgat aaatcttgca
      301 gcgttgggtt tttagattcc cgctttcgcg ggaatgacgg ggcggtcgga tgccgcctga
      361 aactccgtca ttcttgcaac ttttcgtcat tcccgtgaaa acgggaatct agaacttcaa
      421 actttcagat aatctttgaa tattgccgtt gcccgacggt ctagattccc gcttgcgcgg
      481 gaatgacggt agagcggttt cagttgctcc cgataaatgc cgcaatctca aatcccgtca
      541 ttccctcaaa aacagaaaaa caaaatcaga aacctaaaat cccgtcattc ccgcagctct
      601 tcgtcattcc cgtgaaagcg ggaatctaga ccttttaaat tttcacacgg cttttgaata
      661 ttgccgttac ccgaaggtct ggattcccgc ctgcacggga atgacggcag agcggtttca
      721 gttgtttcta aataaatttt tgcagcgttg gatttttaga ttcccgcttt cgcgggaatg
      781 acggggcggt cggatgccgc ctgaagcccg acaaagcccc caatgccgtc tgaaacctcg
      841 tcattcctct gctgtccgca acttttcgtc attcccgcaa aagcgggaat ctagagcctc
      901 taaattttca gacggctttt gaatattgcc gttgcccgac ggtttggatt cccgcctgcg
      961 cgggaatgac ggtggaggac gatgccgtct aaagcccatt agcggtttca gacggcattt
     1021 ttgccggagc ggcagtcagg aaaactgcgc tgcgccacgg tttgccaaat cgtcggcgcg
     1081 ttcgttttcc gcgtgtcccg cgtgtccttt aacccaagtc cagctgactt ggtgctgccc
     1141 aaccagaacg tcgagttctt tccacaagtc gtcgtttttg acgggctgtt tggcggcggt
     1201 tttccagccg ttgcgcttcc aaccgtgtat ccagttttcc atgccgtttt tgacgtattg
     1261 cgagtcggtg cagatgatga cggtgcagcg gcgtttgagc gatttcagtc cttcgatgac
     1321 ggcggtcagc tccatgcggt tgttggtggt ttgcgcctcg ccgccgaaaa gttctttttc
     1381 gtggctgccg tagcgcatta atacgcccca gccgcccgcg ccgggattgc ctttgcacgc
     1441 gccgtcggtg taaaggtaaa cgggtgtgtc catattgtgc ctttgtgtgg tgtccggcgt
     1501 gattataagc ggtttgcgcg ccgtgccgtc tgaaagattg cggcggattg aaataggcat
     1561 ataatgtgca actttatttt tgaatatgaa agagcggata gtgggtcaaa gcggcgagtt
     1621 gttttgtttc ggacaaatgc ctgtatggaa agtggaaaac ctgccggaaa ttttgttgtc
     1681 gggctattcg tctgaggaag gggaatgggt ctgcctgaat gtgttgcagg gcgatgtcga
     1741 agtccgtgca ccggacgggt cggcagaggt ttggtcggca gacggcggcg attgtgtgtt
     1801 tgcgccgcag caggtgtttt cggtcaaacc gaaaacggac gatgccgaaa tccgcttgtc
     1861 gatgtattgc acggcggcag actattttca caaaaaatac gggatgagtg ccacgcattc
     1921 tgcagtgtcg gcggctcagg ataccgtacc tgcgggcagg gcgctcgata tgggctgcgg
     1981 acagggacgc aacgcgctgt ttctcggttt gaaggggttt gaagttactg ccgccgattg
     2041 caatcccgcc gccttggcaa acgtggcgga gctggcagag gcggagggtt tgaacgtccg
     2101 cacgctggaa tatgatttga acgccgccgc cttgcagggt gagtttgatt atattgtggc
     2161 aacagtggtg ctgatgttcc tgatgccgca gcgcgtgcct gacgtgattg ccgatatgca
     2221 ggcgcatacg gcggcgggcg ggtacaattt gattgtatcg gcaatggata cggcggattt
     2281 cccctgcccg atgccgttcc cttttaaatt taaagaggga gagctgaagg attattatcg
     2341 ggattgggag ctggtcgaat ataaagaaga attgggcgcg atgcacgcca aagacgaaaa
     2401 cggcaatccg atacggttta aatttgtaac gatgctggcg aaaaagcctg aataatcctc
     2461 aaaaatgccg tctgaacatt ttgtttcaga cggcattttt acggttagtc ccgataggcc
     2521 caatgattga ccgggccgtg tcccgcgccg atttccaaag ggcttgagat tgccgccgtg
     2581 atgtaggact tggcagtccg tacggctttg caaacgtcta agccttttgc caactcggcg
     2641 gtaatgcagg cagaaaacgt gcagcccgtg ccgtgcgtgt gggcggtcgg aaagcgcggg
     2701 ctgtccaatt ccagcgtttc gttttgcgta aacagccaat ccgtgcagcg tccgctgctg
     2761 ctgccattca aatgtccgcc tttgatgacg acatttttga caccgtaatc aagcaggatt
     2821 tttgccgcac gttccgcatc tttacggttt tcgatatgca cgccggtcag ggcttccgcc
     2881 tcgggcaggt tgggggtcaa tacatccgta tcgggaagca gcaggcgcgt cagtgccgca
     2941 acggcggaat cctgcaacag cggcgcaccg cctttggcaa tcatcacagg gtcgagtacg
     3001 cgcctgccga agtcgcagtg tttcagcttg tcggcaacgc attcgatgat ttccgccgtg
     3061 ccgagcattc ctattttgta ggcgcggatg tcaaagtctt ccctgattgc ttggatttgt
     3121 gcggtgatgg tttcggtcgg gacgagatga accgccgaca cgcccaaggt attttgcgcg
     3181 gtaacggcgg tgatgacgca cgttccgaac acgccgcgca tctgaaacgt tttcaaatcc
     3241 gcctgaatgc ccgcaccgcc gcccgaatcc gaaccggcga tggtaagcgt ttgcacaaaa
     3301 gagcttttca tttgttcctc cttaatattg tgtccgataa aaagggctaa tgcctgtaaa
     3361 aaagcacggc gatgccgtct gaaaaccgca gcgcggggtt tgtggtgcat tatatgccgt
     3421 attgcaggac aaggcacagg gcagggcggc ggggtgcatc gtagcaactg tatttttgcc
     3481 cgacggggtg aaaaatacag ttgctacacc cactgataat gaaaaaagag agcaatgccg
     3541 tctgaaccgc gtgttgcggc gagcataaac cggcacgctt tattcgtcat ttccacaaaa
     3601 acagaaacct aaaattccgt cattcctacg aaagcgggaa tccagtgtgt tcggtttcag
     3661 ttgtttttgg gtttcgggtg atttccaaat catcattccc gcgcaggcgg gaatctagcc
     3721 cttggggcag cggcaatatt tataaagccg tctgaaaatt cagaagttct agattcccgt
     3781 tttcacggga atgacgaagg gttgtgggaa tgacgaagtt tcagacggca tcgccccgcc
     3841 ccgtcattcc cgcataagcg ggaatccaaa ccattgggca gagcagcctt aaaggttata
     3901 aaagactcgt cattcccacg aaagtgggaa tccagacctg tcggcacgga aacttatcgg
     3961 ataaaacggt ttcttcaacc ctacgctcta gattcccgct tttgcgggaa tgacgaagtt
     4021 tcagacggca tcaaatgctt tgtcaggctt cagacagcat cgtccaccct ctgccgtcct
     4081 tcccgcgcag gcgggaatcc aatgtgttcg gtttcggttg tttttgggtt tcgggtaatt
     4141 tccaaatcgt cattcccacg aaagtgggaa tccagaccat tgggcggcgg aaatattcaa
     4201 aagtcgtctg aaaattcaga ggttctagat tcccgttttc acgggaatga cgaagggttg
     4261 tgggaatgac gaagttt
//
LOCUS       DAWFAN010000157         4163 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWFAN010000157 DAWFAN010000000
VERSION     DAWFAN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.157"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(144..941)
                     /locus_tag="WGO65_001748"
     CDS             complement(144..941)
                     /locus_tag="WGO65_001748"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ0328010.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            1096..3945
                     /gene="uvrA"
                     /locus_tag="WGO65_001749"
     CDS             1096..3945
                     /gene="uvrA"
                     /locus_tag="WGO65_001749"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ0328011.1"
                     /translation="MYKHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMESGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAQCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFASVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIACGSPEQVAKVKGSYTGKYLKVVLR"
     gene            3979..>4163
                     /locus_tag="WGO65_001750"
     CDS             3979..>4163
                     /locus_tag="WGO65_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328012.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVY"
BASE COUNT         1077 a         1227 c         1007 g          852 t
ORIGIN      
        1 tctgataaat caccgtaacg ttaaatttcc agattcccat tttcgtgaac ataacggtgt
       61 gaacattagc aaaaattcaa aaaaccaaac ttcctcattc gtgtgaatga tggtactgat
      121 attttctatt tcagttcgca aaattaatct ttcaacacat cttgcacagc cgcctgaatc
      181 ttagccgccg ctgcctcgat ttgccgttgt tcggcagatg ggtttgccgc tgtttcaacc
      241 ggagcagttt gtaaagcagg ctcagactca ggctcggatt cgatttgcgg cgcagtcagc
      301 ggcaaacgcg ccaatacagt gctaacgcca gttactttat cgccaatcgc cacttgcgcc
      361 tgcgcatcga caggcagata catatccacg cgcgaaccga agcggataaa gccataacgt
      421 tcgccgcggg acagtttcgc acctgcttgg gtgtagcaca aaatacggcg cgccaccaaa
      481 ccggccactt gaacaaaagt aatttcacga ccggaagccg tagtcgccaa caccgcatta
      541 cgttcgtttt ccgtgctggc tttgtccaaa tccgcattca cgaatttgcc tttgttatag
      601 accactttcg ttaccgtaca atcggcaggc gatttttgcg aatgcacgtt gaacacgttc
      661 ataaaaatac tgattttcaa agcatcgaca tcacgatacg gatcgcgtgc gcgctcgacc
      721 accacgatac ggccgtcaac cggactcaac actgcttcag gattttgcgg aatctcacgc
      781 gcagggtcgc ggaaaaactg cagggcaaat acggtaaaca cccaaaacgg caaagaccac
      841 cagccgcaac acatcgacac cagcaggctc aaagccaaac cgccgccaat aatcggccag
      901 ccctcacggg cgataatcgg gtgggggtaa agacggttca tttgtcatcc ttttgttcaa
      961 gcgggttaaa attgccgcca ttataacgga atccgccgcg ttccgccgac aaatgccgtc
     1021 tgaacgcggc ttcagacggc atccgagaaa gtgctacaat accagttttc ctttattttt
     1081 caggcggcat ccattatgta caagcatcat ccccaacatt cgcacgacaa cgacaccatc
     1141 cgcatccgcg gcgcgcgcac gcataatttg aaaaacgtcg atttggacat tccgcgccac
     1201 aagctcgtag tggtaacagg cttatcgggc agcggcaaat cgtcgctggc atttgatact
     1261 ttatatgccg aaggtcaacg ccgttatgtc gagagccttt ccgcctatgc gcggcagttt
     1321 ttgcagatga tggacaaacc cgatgtcgat ttgattgaag gtctgtcgcc cgcgatttcc
     1381 atcgagcaga aatccaccag ccacaatccg cgttccaccg tcggcacggt tacggaaatc
     1441 cacgactatc tgcgcctttt gtacgcacgc gtcggcacac cctactgccc cgaacacaag
     1501 ctgccgctat ccagccaaac cgtatctcaa atggttgatg ccgtcttaaa actgccggaa
     1561 gacacgcgcg tgatgattct ggcgccggca gttcgtgagc gtaagggcga gtttgtcgac
     1621 ttctttgccg acttgcaggc gcaaggcttt gcccgtgtgc gcgtggacgg cgaagtttat
     1681 cagcttgacg aagtgccgaa gctggagaaa aacatcaagc acaatatcga cgtggtcatc
     1741 gaccgcgtga aagtgaaggc ggacatcaag cagcggctgg cggaaagttt tgaaaccgca
     1801 ctgcgccacg gcaacgagcg cgcgctggcg atggaaatgg aaagcggcga agaacattgg
     1861 ttttccgcgc gtttcgcctg tcccgtatgt tcatacagcc tgcccgaatt agagccgcgc
     1921 ctgttttcgt tcaacaaccc gatgggctcc tgcccaactt gcgacggctt gggcaacacc
     1981 aacttcttcg atcccgaaaa agtggtcgcg catcccgaat tgtcgctggc aacgggcgcg
     2041 attgacggct gggataagcg caaccagttc tatttccaaa tgattcagtc gctggcgcgg
     2101 cattacggtt tcgatgtgca ggctgcttgg gaaacgctgc ctgaaaaagt caaaaaagtc
     2161 gtgctgcacg gctcgggcaa ggaagtcatt gatttcacct acctatccga acgcggcacc
     2221 accttcaacc gcagccacgc cttcgaaggc atcattccca atctcgaacg ccgctaccgc
     2281 gaaaccgaca gcgaaaccgt gcgcgaaaaa ctgcgtgaat accagaacca ccgcgcctgc
     2341 ccgagctgcg gtggtgcacg tttgcgcaaa gaagcccgct acgtttacgt cagcggcgaa
     2401 ccgctgcacg aagtttccgc ctggccgctc accaaaaccc accaattctt tgaaacactg
     2461 gatttagacg gcaacaaaaa acaaatcgcc gaaaaaatcc tcaaagaaat caccgaacgc
     2521 ctcggcttcc tgattaacgt cgggctggat tacctgaatt taagccgctc cgccgaaacc
     2581 ctctccggcg gcgaagccca gcgcatccgc ctcgccagcc aaatcggcag cggcctgacc
     2641 ggcgtgatgt atgtactgga cgaaccctcc atcggcctgc accagcgtga caatgaccgc
     2701 ctgctggcca ccctcaaacg actgcgcgat ttgggcaaca gcgtgattgt ggtcgaacac
     2761 gacgaagacg ccatccgcga agccgatttc gtggtcgata tgggccccgg cgcaggcgaa
     2821 cacggcggca acgtactgat tgccgacacg cccgaaaacg tcgcccaatg cgaaaactcc
     2881 gttaccggac aatacctcag cggcaaaaaa tccattgccg tgccgtctga acgcacgccc
     2941 gtcaatcccg accggatgct cgtcctcaaa ggcgcacgcg gcaacaatct caaaaacgtt
     3001 acccttgagc tgccgctcgg tttgattacc tgcatcaccg gcgtatccgg cagcggcaaa
     3061 tccaccctga ttaacgacac cctcgccaaa attaccgccc gcgaactcaa ccgcgcccaa
     3121 gaagaacccg ccccatacga cgacatccgc ggcctcgaac acctcgacaa agtcatcaac
     3181 gtcgaccaat cccccatcgg ccgcacccca cgctccaatc ccgccaccta caccggcctg
     3241 ttcaccccca tccgcgaact cttcgccagc gtacccctct cgcgtgaacg aggctacaac
     3301 gtcggcagat tctccttcaa cgtcaaaggc ggccgctgcg aagcctgcca aggcgacggc
     3361 gtgattaaag tcgaaatgca cttcctgccc gacgtgtacg tcccctgcga agtctgccac
     3421 ggcaaacgct acaaccgcga aacgctcgaa atccaataca aaggcaaaaa catcagccaa
     3481 gtcctcgaca tgaccgtcga agaagcccgc gaatttttcg acgccgtccc caccgtatcg
     3541 cgcaaactgc aaaccctgat ggacgtaggt ctcggctaca tccgcctcgg ccaatccgcc
     3601 accaccctct ccggcggcga agcccagcgc gtcaaactcg ccttggaact ctccaaacgc
     3661 gacaccggca gaacactcta catcctcgac gaacccacca ccggcctaca cttcgccgac
     3721 atcgccctgc tgctggaagt cataggtcgt ctgaaaggca aaggcaactc gattgtgatt
     3781 atcgagcata atctagacgt gattaaaacc gccgattgga ttgtggactt agggccggaa
     3841 ggcggcgatg gcgggggcag gattattgct tgtggtagcc ctgagcaggt ggcgaaagtt
     3901 aaggggagtt atactgggaa gtatttgaag gtggttttga ggtaagttta attcaaaatt
     3961 aaataggaga ttattaagat gaaaccgtta aaaacattag aatttggatt tgtggatgct
     4021 gcaaactaca gaagaagaga aaataaagat ttatttaacc gaatatttgt aaaaggagaa
     4081 tatttggatg aattatgtga accaaatatt tcgtttttaa tcggagaaaa gggaactgga
     4141 aagacagcat atgctgttta ttt
//
LOCUS       DAWFAN010000158         4114 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWFAN010000158 DAWFAN010000000
VERSION     DAWFAN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.158"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..143
                     /locus_tag="WGO65_001751"
     CDS             <1..143
                     /locus_tag="WGO65_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ0328013.1"
                     /translation="VGIGGGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRHI
                     QT"
     gene            273..1709
                     /locus_tag="WGO65_001752"
     CDS             273..1709
                     /locus_tag="WGO65_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ0328014.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQQADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1720..2703
                     /locus_tag="WGO65_001753"
     CDS             1720..2703
                     /locus_tag="WGO65_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ0328015.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2700..3893
                     /locus_tag="WGO65_001754"
     CDS             2700..3893
                     /locus_tag="WGO65_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ0328016.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRIA
                     RQGQTMFWSWQNAMGGVCSMKNWLNHGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
BASE COUNT          878 a         1343 c         1053 g          840 t
ORIGIN      
        1 acgtcggtat cggcggcggc gcgctgctgg ggcattgggt tacgcaatat gtcggcattt
       61 cccgtatcgg catcgccggt atgcttgcgg cggcggccgg gctgtggctg tgcctgaact
      121 tgaaccgcca tatccaaacc tgaccgcgcg atgccgtctg aagcccctcc cgcccttcag
      181 acggcatttt gattgaagaa atatccgccc ctgcttaaaa taacgggctt tgccgtgttt
      241 taacgcctat tttcttattc caaggatggt ttatgccgct gctgtctgtc gagttcgcac
      301 tgttctttct cgccttcctg ccgatttact ggggcttggc gaaatacccg tccgtccaaa
      361 acctgctgct tttggctgcc ggtatgggct ggctctacca tatcggcccc gtatttgcgg
      421 caatcgtcgt cctttattcc tcctgcgtgt accttttggg cgaactgctc cgttccgatc
      481 gcgaaaatac gcgccgcttt tggctggggt gcggcattgc cgtctcgctg accgtcttgg
      541 gctttttcaa atatttcgat tttttccgcc cgctgattgc acaatatgcc ggacaaggcg
      601 gcgcaatcga catcctgatg ccgctggggc tttcgtatta caccttccag tcaatcgcct
      661 atttggttta ctgcttccgt gcgccgcacg ccgcgcgttt cgagtggcac gaactgctgc
      721 tgcacctgag ttttttcccc accgttacct ccggcccgat tatccgcgcc gccgcattca
      781 aaagcgcgga cggcgagcag gcgggcgcat tggcgcaaat ccgtacccac caaccacgct
      841 cacccgtccg ccccgcactc gccgtttccc tgattctgct gggtattgcc aaaaaatggt
      901 ggctggcggg gatgctcgca gaaaactggg tatcgcccgt atttgaaaat cccgcccaat
      961 tcgacggctg gggcgtattg gcgggcgtgt acggctatac cttccaactc tttttagact
     1021 tttccggata ttccgacttg gttatcggaa tggcaatgct gctgggcttc cggctgccca
     1081 aaaacttctc cgcaccgctt cgtgccgcga atatccgcgc attttgggac aaatggcaca
     1141 tcagcctttc cacctggata cgcgactaca tctacatccc cttgggcggc agcaaaaaag
     1201 gctttttgcg gacacagttc aacctgatgg cggcaatggt gctctcaggc atctggcacg
     1261 gctacggctg gaacttcctc atttggggtg cactacacgg cacggcgctg gtgctgctca
     1321 acacgggcga ccgctatttc ggacgcaacg cactatgccg tctgaaatac cttgcgccgc
     1381 tctcgtggtt tgttaccttc catttcgtct gcctgacctt cgtcgtcttc aataccgcaa
     1441 accccgacga tgccggcgcg gttttcaatg ccctctttgc caatgccaac ggctggaatg
     1501 cgccgcaaca ggcggatatg ctgctgcttg cctcgtttgc atccgtgatg ctgctctacc
     1561 cttacctgca acgcgctttc gacggcgcgg tcaaaggttt ggaaaaaatc ccgatgtggc
     1621 tgtggtttat ccccgtttcc gccgtcctgc tgctgattat cgtattggcc ccctcgggga
     1681 tacccggctt tatttatgcc aatttttaag ggtttggaca tgaaaaactt tctttccctt
     1741 ttcgcctcca tactgatgtc tgccctgatt gccgtgtggt tcagccaaaa ccccatcaac
     1801 gcctactggc agcagaccta ccaccgcaac agtccgctcg aaccgcttgc cgcctacgga
     1861 tggtggcgga gcggtgtggc gttgcaagaa aacgcctacg ccctttcaga cggcatcaaa
     1921 gccttcctgt ccggcgaaac gccgccgacg gctcaagacg gcggttcggc agatatgccg
     1981 tctgaagccg ccgcatcaga agccgtccct caaaccggtg aaacagaatg gaaacaagac
     2041 accgaagccg ccgccgtccg cagcggcgac aaagtctttt ttgccggcga ctcgctgatg
     2101 cagggcgttg cacccttcgt gcaaaaaagc ctgaaacagc aatacggcat cgaatccgtc
     2161 aacctcagca aacaaagcac ggggctgtcc tacccctcat tcttcgactg gccgaaaacg
     2221 attgaagaaa ccctgaaaaa acatcccgaa atcagcgtgc tcgccgtctt cctcggcccg
     2281 aacgacccgt gggatttccc cgtcggcaaa cgatacctca aattcgcttc cgacgaatgg
     2341 gcgcaagaat acctgaaacg cgtcgaccgc atccttgaag ccgcacacac gcaccgcgtc
     2401 caagtcgtct ggctcggcat cccctatatg aaaaaagcca agctcgacgg gcagatgcgc
     2461 tacctcgaca aactgctttc ggaacacctg gaaggcaaaa tcatcctgat tcccaccgcg
     2521 cacaccctga gcggcgggga agaccgctac accgactccg tcaacgtcaa cggcaaaccc
     2581 gtccgctacc gcagcaagga cggcatacac tttaccgccg aaggacaaaa actgctggcg
     2641 gcaaaaataa tggaaaaaat cgtttttgaa ccaagtacgc aaccatcaag tacacagcca
     2701 tgaaccccaa acacctcatc gcattttccg ccctgttcgc cgccacgcag gcagaagccc
     2761 tgcccgtcgc ctccgtcagc ctcgacaccg ttaccgtttc cccgtccgcc ccctacaccg
     2821 atacaaacgg gctcttgacc gactacggca acgccgccgc ctcgccttgg atgaaaaaac
     2881 tccgatccgt cgcacaaggc agcggcgaga ccttccgcat cctgcaaatc ggcgactcgc
     2941 ataccgccgg cgacttcttt accgacagcc tgcgcaaacg cctgcaaaaa acttggggcg
     3001 acggcggcat aggctgggtt taccccgcca acgtcaaagg gcagcgcatg gcggccgtcc
     3061 ggcacaacgg taactggcaa agcctcacca gcaggaacaa tagcggagac ttcccgctcg
     3121 gcggcatcct cgcccacacc ggcagcggcg gcagcatgac cctgaccgca tcggacggca
     3181 agaccggcaa acagcgcatc tccctgtttg ccaaacccct gctcgccgaa caaaccctga
     3241 ccgtcaacgg caacaccgtc tccgccaacg gcggcggctg gcaggtactg gatacgggcg
     3301 cggcactccc actgaccata cagaccgaaa tgccgtggga catcggcttc atcaacatcg
     3361 aaaatcccgc cggcggcatt accgtttccg ccatgggcat caacggcgca caattgaccc
     3421 attggtcgaa atggcgtgcc gaccgtatga acgaccttgc ccaaaccggc gccgatttgg
     3481 ttatcctttc ctacggcacc aacgaagcct tcggcgacaa catcgacatt gccgacactg
     3541 aacaaaaatg gctggatacc gtccgccaaa tccgcgacag cctgcctgcc gccggcatcc
     3601 tcatcatcgg cgcacccgaa tccctgaaaa acacgctcgg cgtatgcggc acacgccccg
     3661 tccgcctgac cgaagtccaa cagatgcagc ggcgcatcgc ccgtcagggg cagacgatgt
     3721 tctggtcttg gcaaaacgcg atgggcggcg tttgcagcat gaaaaactgg ctcaaccacg
     3781 gatgggccgc caaagacggc gtacacttct ccgccaaagg ctaccggcgc gcggcggaaa
     3841 tgctcgccga cagcctcgaa gaactcgtcc gcgccgccgc aatcaggcaa taatcggaca
     3901 gggggcggac ggtatttccg caacaggggg atgccgtctg aaacgcatac cttcatattg
     3961 cttcagacgg catagccacc ccgcgcacgg tttgccagat gcaaccggca ttcgcctcag
     4021 gcatcggaag gacgcaggcg aacctccggc atacggcgca aaggcggcgt ttgatatgcc
     4081 gtctgaaggc aaagatgata aactgccgcc ttcc
//
LOCUS       DAWFAN010000159         4019 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWFAN010000159 DAWFAN010000000
VERSION     DAWFAN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.159"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(1062..1451)
                     /locus_tag="WGO65_001755"
     CDS             complement(1062..1451)
                     /locus_tag="WGO65_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ0328017.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILLVLQELEKQAAR"
     gene            complement(2024..2149)
                     /locus_tag="WGO65_001756"
                     /pseudo
     CDS             complement(2024..2149)
                     /locus_tag="WGO65_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(2195..3328)
                     /gene="carA"
                     /locus_tag="WGO65_001757"
     CDS             complement(2195..3328)
                     /gene="carA"
                     /locus_tag="WGO65_001757"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ0328018.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYASGLIIRDLPLLHSNFRASESLHDYL
                     VRNQTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKSFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT          908 a         1036 c         1050 g         1025 t
ORIGIN      
        1 aaaataacgg cgtattgcct ttgattataa ttaaaaggaa atgcttcaat tattctattt
       61 ttgtttctca tatcattaag caaatacttt aaattattga aattaatagg tattgacata
      121 tttaaactcc tgcgaatatg ttgcaataaa cgtagcaatc gtaggttggg ttgaaaaccc
      181 aacacaaacc gcttaattgt ctaaaatgtt gggtttgcta acctaacttg tatatcgaaa
      241 cccccgtatt gtagcaagcc gtagcccgta tgtagggtat gtgcggtacg tatgcacgcg
      301 ttctccattt tccctgcaac cccaacccac cgtgtgcgtg ccttgcggca cacaccctac
      361 cgatgggttc aaaggtcgtc tgaaaaaacg ctcgcggcat ttgttccatc ttccttgcga
      421 taaaggctac ctgaaaaatt cctgctgttg atatctcggg cattctttct caaaccgcct
      481 gcaactgcca aatctgctgc aacctgcccc ctcccccgtg ggggagggtt ggggagaggg
      541 cattccccga atttcggcaa cctttcccaa caactcaacc accgaaatac aagccttgtg
      601 gcttgttgcc ctctctctag ctctctccca cgggagagag gacggagtgg ctgtagggct
      661 aagggttctg caaggaaaca ggttgtgcag gttgcttttt gcttttcaga cgacctgaaa
      721 tcaacggtaa atccaagtcg cttttcaaac cgcctgcaac cgcaaaatct gctgaaacct
      781 actccctccc ccgtggggga gggctgggga gagggcattc tccgaatttc ggcaaccttt
      841 cctaacactc taaccgccaa gacacaagcc ttgtggcttg ttgccctctc tctaactctc
      901 tcccacggga gagaggactg ggcggttgtt ggggctaagg tttctgtaaa ctaatcaggt
      961 tatgcaggct gttttttgtt tttcagacgg cctttataaa tgctcaattc agtcggcttt
     1021 atcctattat tttcaataat ccaatcaaaa tcagccatct gctatcgtgc agcttgcttt
     1081 tccaattctt gcaacacgag caggatttcc gccagtacat cgtttgtctg ctgcaaaatt
     1141 tcgtgattcc aaaaacgcag cacggtaaag cccaggctgt tgagatatgc cgtccgcgcg
     1201 tggtcgtata cggcttgttc cgcgtgctgc cctccgtctg cttcgacaat caacttgggc
     1261 gttacgcaca taaaatcaac aatatagttt cccatcggct gctggcggcg gaatttatag
     1321 ccgttcagac gacctgcccg caggtgctgc cacaattttg cttccgcccc gctcatttct
     1381 tggcgcatgg ctttggcgcg ttggcgcaag atggggttgt cggtggtcaa gagtttctcg
     1441 tgcgggttca ttttgttgtc gtgctgtttg ctgccctctc tctaactctc tcccacgggg
     1501 agagaggacg gggcggttgt tggggttaaa ggggctgtaa ggaaaacaag tcgttcaggc
     1561 cactttttac ttttcagacg acctgcaatc aacggttgta aatccaagtt gcttttcaaa
     1621 ccgcctgtaa ccgcaaaact tgctgcaacc taccccctcc cccgtggggg agggctgggg
     1681 agagggcatt ctccgaatcg aggcaatctt tccctaattc cctaactgcc tcaatacaag
     1741 cctttcggct tgttgccctc tctctaacgc tttcccacgg gagagaggac ggtgcggctg
     1801 tagtgattaa gggttttgca aggaaaacaa ggaaaacaag ttgtacaggc tgttttttat
     1861 ttttcaagcg acctgaaatc aacgcttgta aatccaagtc gcttttcaaa ctgcctgcaa
     1921 ccgcaaaatc tgctgcaacc taccccctcc cccgtggggg agggctgggg agagggcatt
     1981 ttccaagttg cggcaatctt tcccaaatcc cttagtcttc taaaatacaa gccttacggt
     2041 tgttgccaaa tgaaacttct tccataccca aaacttgcgt gtaaaacgcg atggttcggt
     2101 caatgtcggc aacggtcagt accagatggt cgagtgcgct aatcttcatt tcccgctttc
     2161 tttttcaggc ggcggcagtg ccgtctgaaa gccattatgc ccgttttgcc gctttcatat
     2221 tgccaatgaa tttgtcaaac aaatagccga catcttgcgg accggggctg gcttcggggt
     2281 ggccttggaa gcagaatacg ggtttgtcgg tcagctcgat gccttgcaaa gtgttgtcaa
     2341 acaaggattt gtgggtaatc cttgcgttgg cgggcagggt gtcggcatcg acggcgaaac
     2401 cgtggttttg gctggtgatg acgactttgc cgctgtccaa atcttgcacg gggtggttcg
     2461 cgccgtggtg gctgaagcgc atttttaagg ttttcgcgcc gatagcgagg ctgatgagct
     2521 ggtgtcccaa gcagatgccg aaaatcggtt tgccgctttc catcagtttt tgcacggctt
     2581 cgatggcgta ggtgcaaggc tcggggtcgc cggggccgtt ggacaggaac acgccgtcgg
     2641 gattgagtgc caacacgtct tgtgcgctcg tttgggcggg gacgacggtc aggcggcagc
     2701 cgcgcgaggc gagcatacgc aggatgttgg ttttcacgcc gaaatcgtag gcgacgacgt
     2761 ggtaaggctg ttcgtcaggg gtaacgaaac tcttgcccaa ttcccattcg ccttccgtcc
     2821 attcgtaagt ttccgtgcag gaaacttctt ttgccaagtc tttgccgacc atactgccga
     2881 acgcggcgat gagttcttgc gctttttcga ttgtggcatc cgcaccggtc agaatcgcgc
     2941 cgccttgcgc gcctttttcg cgcaacagcg tggtcaggcg gcgggtgtcg atgtcggcga
     3001 tggcgacggt ttggttgcgt accaaatagt cgtgcaggct ttcggaggcg cggaagttgc
     3061 tgtgcaagag cggcaggtcg cggataatca ggccggaggc ataaacgctg cggctttctt
     3121 catcttcggc gttggtgccg gtgttgccga tgtgggggta ggtgagggta acgatttgtt
     3181 tgcagtagga cgggtcggtc aggatttcct gatagccggt catcgaagta ttgaacacga
     3241 cttcgccgga agtcgaacct tcgtaaccga ttgatgtgcc gtgaaatacg ctgccgtcag
     3301 cgaggacgag gagggcgggg gtgctcatga tgggaatcct gtttttaata aaattcttga
     3361 caatgccgtc tgaagggact tcggcagggg gcatccggtc ggaaaaatcc ggtcaaaaaa
     3421 aaacacgccg cgcaagatag acgcgtaacg tgcttttcgg gcaagtgcct ataccctgaa
     3481 agcagcatat tttaagacga aacatacccg atttcaagcg caaacgcggc aggcgggctg
     3541 atatgttcgc gcgaaaacgg aaatccgtgg cgtaaaaccg cccgctctgc gcccactatg
     3601 cccgttacgc atttccccac gtccgtccgg cgaaactatg ggaatacccg aatcgtcagg
     3661 aatacctgaa tcgtcaggaa tacctgaatc gtcaggaata cccgaaccgt cattcccgcg
     3721 caggcgggaa tccagacctg tcggcacaga aacttatcgg gtacaacggt ttcttgagat
     3781 tttacgtcct agattcccgc ctgcgcggga atgacgcgtt tcatttgccg ccccctcccg
     3841 aaaaacgcaa aaaatgccgt ccgaaaacct ttcggacggc attttcgcgt gaaccgtcat
     3901 tcccacgaaa acagaaaacc aaaatcagaa acctaaaatt cgtcattccc acgaaagtgg
     3961 gaatccagga ctcaggacct gagaaacctt tttacccgat aagttcccgt accgaccgg
//
LOCUS       DAWFAN010000160         3988 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWFAN010000160 DAWFAN010000000
VERSION     DAWFAN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.160"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="WGO65_001758"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="WGO65_001758"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ0328019.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="WGO65_001759"
     CDS             1160..2290
                     /locus_tag="WGO65_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ0328020.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTVEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2361..3212
                     /locus_tag="WGO65_001760"
     CDS             2361..3212
                     /locus_tag="WGO65_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ0328021.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="WGO65_001761"
     CDS             complement(3295..3957)
                     /locus_tag="WGO65_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ0328022.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEEFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          868 a         1175 c          983 g          962 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccgg acaaacgggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaccctgcc
      181 gtcgatgatg tgcgtggctt tcacgccgtt gacggcggct tcgaccgcgg aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 gagtttttca gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccgcgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatgatcgtc
      601 gcgtccgctt acgccgaccg cgtgcccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacgtgc ccgcccaaca ccatttcgac aatatccatc gtctctttgt cggtcacgcg
      721 cattccttgg acaaactcgc cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgccg tgaacgataa cgggatggat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtact tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgctttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacgactccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatctggca gaccttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgacc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaat catacgccgc tgtccaacag cgtgtattac gcctatttcg aaccttactc
     1501 cgaagaacag catttaaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aaggccgcga catcaatctg ctgaccatcg gcaatcaggt
     1621 cgaaagcgat ctgaaaatct ggattaccgc ccgccagcat ccgggcgaaa ccatggcgga
     1681 atggtttgtc gaggggctgc tcggcaggct gctcgaccct caggatgcaa ccgcgcgcac
     1741 cctgctcgat cgcgccacgt tctatatcgt ccccaacatg aaccccgacg gttcggcact
     1801 gggcaacctt cgtaccaacg ccgccggtgc gaacctcaac cgcgaatggg aaaatccgac
     1861 tgtggaaaaa agcccggaag tgttcttcgt gcgcgaaaaa atgctggaaa ccggcgtgga
     1921 tttgttcttg gacatccacg gcgacgaagg gctgcccttt atctttgttg caggaactga
     1981 aggcgtgcca aactacaatc cgcgcatttc cgcgttggaa acacagttca aaaccgccct
     2041 tttgaacgcc agccccgatt tccaagacga atacggttac gaaaaagatg cgcccggtca
     2101 ggcaaacatg accttggcaa caaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gttggaaatg ccgttcaaag acaatgccaa cctgcccgac gacgatttcg gatggaacgg
     2221 tcaacggtcg ttgcgcttgg gcgaagccgc gttgtccgcc atcctcaaca tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcccgtt atgattaccc atccccaatt cgaccccgtc cttatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgctcg
     2521 acgacttcct gacatggggc attttgggcg taattttggg cgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatatgg ttgttcggcc
     2701 gcaaacacgg catcggcttc ctcaaactga tggacacggt cgcaccgctc gttccgctgg
     2761 gtctcgcttc gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagat gccgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcgccgtc gtttggctgt
     3001 tctctaaaaa acagcggccg accggacaag tcgcctcgct cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa ttcgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gcatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 tgttttttcg ccttccctgc ggacggtttg cggtttgttg atgactgcct gccagtagcg
     3421 gtagatatcc gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt
     3481 gaattgcaga cggatagcgc cgttttcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa attcttcatc atcggcggct tcaaaccaat cggtcggcac
     3601 aatatccaag ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aattttcggg aatccatcat
     3961 tttccttttc aaatgggttt tgcgccct
//
LOCUS       DAWFAN010000161         3984 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWFAN010000161 DAWFAN010000000
VERSION     DAWFAN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.161"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            272..1789
                     /locus_tag="WGO65_001762"
     CDS             272..1789
                     /locus_tag="WGO65_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0328023.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALSQVWRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRKLHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPVPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQDGGS
                     QICSVSRAERATGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLSPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1848..3629)
                     /locus_tag="WGO65_001763"
     CDS             complement(1848..3629)
                     /locus_tag="WGO65_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ0328024.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGIVATEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADILRAAFPCGSITGAPKKMSMQIIETLETEARGLYTGSIGYLNPCSGGLGFEGAFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENRQCALLDRHLCRLKAAAQALNLPLPDGCENQIKQYIARLPDGVFRVKALLASDG
                     ISLSRAVLNHLADKQRVIISPTILPAQNYLRHFKTTHRALFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLHTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          844 a          888 c         1294 g          958 t
ORIGIN      
        1 ctcgccttag ctcaaagaga acaattctct aaggtgttga agcaccaagt gaatcggttt
       61 cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccgctat
      121 aaatgtgaaa tccgcccttt gaaaatcggg cggcggtttt ttgcctgccc gctttcagac
      181 ggcaaagccg gttttcacgg ttttctgcct gtttttcgga tggtttgacg tgtttcggcg
      241 gcgtgtttgc cggaaaggta aatgacagga tatgttgtat ttcagatacg gttttttggt
      301 tgtttggtgt gcggcaggtg tttctgccgc ctatggggcg gatgcgcctg caattttgga
      361 tgacaaggct ttgtcgcagg tgtggcggtc ggtgtcggat aaatgggcgg aatcggattg
      421 gaaagttgaa aatgatgccc cgcgcgtggt tgacggggat tttttgttgg cgcacccgaa
      481 aatgttggaa catagtttgc gcgacgcgct caacggcaat caggcggatt taatcgcttc
      541 gttggcggat ttgtatgcca agctgccgga ttatgatgca gttttgtacg gcagggcgcg
      601 ggctttgctg gcgaaattgg cgggaaggcc ggcggaggcg gtggcgcggt atcggaagct
      661 gcacggggaa aatgcggcag acgagcggat tttgctggat ttggcggcgg cggagtttga
      721 cgatttccgg ctgaagtcgg cagaaagaca ttttgcggag gcggcaaaat tggatttgcc
      781 ggtgccggtt ttggaaaatg tggggcgttt tcggaaaaaa acggaggggc tgacgggctg
      841 gcgtttttcg ggcggcatca gtccggcggt caatagaaat gccaataatg ccgcgccgca
      901 atattgccgg caagacggag gcagtcagat atgcagtgtc agccgggcgg agcgggcgac
      961 aggcttgaat tatgaaatcg aggcggaaaa gctgacggcg ttggcggaca atcattattt
     1021 gttgttccgt tccaatatcg gcggcacaag ctattatttc agtaaaaaat cggcttatga
     1081 cgacgggttc ggcagggcgt atttgggatg gcagtataaa aatgcgcggc agacggcggg
     1141 gattttgccg ttttatcagg tgcagttgtc gggcagcgac ggctttgatg cgaaaacaaa
     1201 acgggtaaac aaccgccgcc tgtcgccgta tatgctggcg cacggagtcg gcgtgcagct
     1261 gtcccatact taccgcccaa acccgggatg gcaattttcg gtcgcgctgg aacattaccg
     1321 ccaacgctac cgcgaacagg atagggcgga atacaataac ggcaggcagg acgggtttta
     1381 tgtttcgtcg gcaaaacgtt tgggcgaatc ggcaactgtg ttcggcggct ggcagtttgt
     1441 gcggtttgtg ccgaaacgtg aaacggtggg cggcgcggtc aataatgccg cctaccggcg
     1501 caacggtgtt tatgccggct gggcgcagga gtggcggcag ttgggcggtt tgaacagtcg
     1561 ggtttccgcg tcttatgccc gccgcaacta taagggcatt gcggctttct cgacagaggc
     1621 gcaacgcaac cgcgaatgga atgtctcgct ggctttgagc cacgacaagt tgtcgtacaa
     1681 aggtatcgtg ccggcgttga attatcgttt tggtaggacg gaaagtaatg tgccgtatgc
     1741 gaaacgccgc aacagcgagg tgtttgtgtc ggcggattgg cggttttgaa tggtgggata
     1801 atgccgtccg aactttggaa acaggttcgg acggcatttt tgcgcgttca ggcaagggcg
     1861 gcggcaaata cgccgcgcaa ggcgttggag agacggattt cttcggcttc ttgcagtgtt
     1921 ttttgtgtga tgtgtgtttc gatcacttga tttgtgtgca aatatttttg cggttcgtcc
     1981 aatacggctt ggcgcattat gccgtttaaa atgtctaaat ctaaagaggg tgtgagccat
     2041 tgtccgcgat gtttgacgaa gacgttgctt ctgccgcctt cgagcaggat gccgtctgaa
     2101 ttgaaaaaca ggctgtcgaa cgcgccttgt gtttcggcgg tttgccacgc ttggtcgaag
     2161 agggcgcggt gagtggtttt gaagtggcgc aggtagtttt gtgcgggcag gatggtcggc
     2221 gaaatgatga cgcgctgttt gtcagccaaa tggtttaaaa cggcgcggga caggctgatg
     2281 ccgtctgaag cgagcagggc tttgacgcgg aacacgccgt cgggcaagcg ggcgatgtat
     2341 tgtttgattt gattttcgca accgtcgggc aggggcaggt tgagggcttg ggcggcggct
     2401 ttcagacggc ataggtggcg gtcgagcagg gcgcattgcc tgttttccac gcgcagggtt
     2461 tcaaaaatgc cgaaatcggg gcgcaattcg ttgaggaaac gggctttcca gccgcattcg
     2521 cgatattcgg cggcggggtc gctgtcgatg acgatgccgg aaccgacacc gtacacgcct
     2581 tgataaatgc cgtctgaaag cggcgtgagc gataaggtgc ggataacgac gttgaacgcg
     2641 ccttcaaacc ccaagccgcc ggaacacggg ttcaaatagc cgatgctgcc cgtataaagt
     2701 ccgcgtgctt cggtttcgag tgtttcgata atctgcatac tcattttttt gggcgcgccg
     2761 gtgatactgc cgcagggaaa ggcggcgcgg aggatgtcgg cgaacgaggt gtccggcagg
     2821 gcttgcgccc ggatggtgct ggtcatctgc cagacgctgc cgaaacgcga tactttaaac
     2881 ggttcgggta cgcatactgt gccggtttgg gcgattttgc cgagatcgtt acgcagcaaa
     2941 tcgacaatca tcacgttttc ggcgcggttt ttcgggtctg cttgcaactc ggcggcgcgg
     3001 cgttcgtctt gtccgtcgcc caaaatcggc gcggtgcctt tcatcggttc ggtggcgacg
     3061 atgccgtctg aagcgatgtt gaggaagagt tcgggcgaga aacacagtgt ccacgcggat
     3121 tgcccctccg catcgggcag gtgggacaaa acggcatagg ggacgggctg gcgcaggcgg
     3181 cggtagaggc tgacgggatt gccgtaggct tgcaggtgca ggcgggtagt gtagttgatt
     3241 tgataggtgt cgccgcgccg gatggcttcg tggatttggc ggatatggtc gaggtaatcg
     3301 gcttcggata cggaggattg cggcgtggaa atgccggcgg ggaggccgtc tgaatgtcgg
     3361 gcaagccagc ttgcggcatc ggtgtcggcg cagtcggcaa accagtgcag ggcaagattg
     3421 ccgccgcgtt cggactcaac ccccgtcagc ggcagaccga atccgtagtc tgcaaacaac
     3481 accgaatgca gccctttttg ccagcccgat tgcagcgcgc cgtccaaagc atcgagttct
     3541 tcgggacgga aaaaacggct ttctacatga ttttgatagc gttttgcgcg accgcttacg
     3601 gcatcgtcaa acagggcgaa atagggcatg gcaattcggg gcaaatgttt tgattatacg
     3661 ctctttttac acatattttc agacgattcg gcaaatatcg gcaaaatgta attttatgta
     3721 gagaaagtgg ggggcgaggg tgtaaaattg tgaaaacaaa ttccgatttc caacctgaaa
     3781 tacaataagg agacctttat gacagaccac cagctgcaac cgtttgaaaa cgtagaatta
     3841 ggcgaaaagc aagaccagct ccaagtatag tggattaaca aaaaccagta cagcgttgcc
     3901 tcgctttagc tcaaagagaa cgattctctc aggtgctgaa gcaccaagtg aatcggttcc
     3961 gtactatttg tactgtctgc ggct
//
LOCUS       DAWFAN010000162         3930 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWFAN010000162 DAWFAN010000000
VERSION     DAWFAN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.162"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..331)
                     /locus_tag="WGO65_001764"
     CDS             complement(<1..331)
                     /locus_tag="WGO65_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0328025.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVKEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQ"
     gene            complement(312..593)
                     /locus_tag="WGO65_001765"
     CDS             complement(312..593)
                     /locus_tag="WGO65_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ0328026.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(602..1396)
                     /locus_tag="WGO65_001766"
     CDS             complement(602..1396)
                     /locus_tag="WGO65_001766"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ0328027.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1780..3114
                     /gene="gdhA"
                     /locus_tag="WGO65_001767"
     CDS             1780..3114
                     /gene="gdhA"
                     /locus_tag="WGO65_001767"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ0328028.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3256..3630
                     /locus_tag="WGO65_001768"
     CDS             3256..3630
                     /locus_tag="WGO65_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328029.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3674..>3930
                     /locus_tag="WGO65_001769"
     CDS             3674..>3930
                     /locus_tag="WGO65_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ0328030.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT         1069 a         1014 c          926 g          921 t
ORIGIN      
        1 cttgcgcgat gccgttcaag gacaaaccca tacccttttc ggcaaaacgg atttcacggt
       61 cgtcaaacga gactttgccg aagccgaccc gtttcagggc ttccttcacg ctgttttgag
      121 gaggcggcgt ttccgcatcg ggacgggcgg caaaataatc ggcatacagt ttccacaacg
      181 cctgcactgt cggatcgaac gcgccgtccg tcagcgcgtg aatatcgcgg cacaggctca
      241 acagttccaa aaaatccgcc gacggcgaag tcagataacc gtccctgttc aggcggctga
      301 tcaggctgtc ttcacggtaa aggctgaaca attttttcca aacgcgccac ttccgccaaa
      361 accttgttga ccaaatccgc cgcctgtctg tcgtccacac cgaacaggcg cagctccgca
      421 ccggaaccca gcgcgacacc tttccagaag aacacatttt cattgcgttt ttcatcttta
      481 ttgcgttttt catcatcggc ggcaaaagga ttcggcagga aagaaaccgc cgcgcccgcc
      541 gccgcaacgg cggcaactgt caaaaaacgc ctgcgcccga aatgcctgcc cataccgcct
      601 cctaaaccga cactgccgcc ttgatgtgcg gatgggggtc gtaaccttcc aactcgaaat
      661 cttcaaactt gaaggaaaac aaatctttga cttcaggatt gattttcatc actggcaagg
      721 cgcgcggttc gcgttccaac tgcaatgcgg cctgctcgaa atggttgcgg tacaaatgcg
      781 cgtcgccaaa cgtatggaca aactcgcccg cctccaatcc gcacacttgc gccatcatca
      841 tggtcaacaa tgcgtagctg gcaatattaa acggcacacc aaggaaaata tctgcactac
      901 gctggtaaag ctggcaggac agtttgccgt cggcaacgta aaactgaaac agcgcgtggc
      961 agggcggcaa ggccatttcg tcgaccaaag ccggattcca cgccgataca atcaggcggc
     1021 gcgagtcggg gtttttctta atttgttcca ccacattggc gatttggtcg atatgcctgc
     1081 cgtcgggcgc gggccagtta cgccactggt agccgtaaac cgggcccaag tcgccgtttt
     1141 cgtccgccca ctcgtcccaa atggaaacat tgttgtcctt taggtatttg atattggtat
     1201 cgcctttgag aaaccaaagc agctcgtgga taatcgaacg cagatgcagc tttttggtcg
     1261 tcagcagcgg aaaacctttg cccaagtcaa aacgcatctg ataaccgaat acggagcgcg
     1321 tgcccgtacc ggtgcggtct gatttgtccg taccgttgtc gaggacgtgg cgcatcaagt
     1381 ccaaataggc tttcatagca gtctttcatc aaattaaacg gcgcatattg taacatttcc
     1441 ggataatgcc caaaacacgg atacagacag gcaggattgt tggcaatttc agtccttttc
     1501 cacagtaaaa cccggtggga aaacaaaatt accttgattg gaatcaaaaa atctagttta
     1561 attacttaga ataaaatttc aataatattt ttatttacga aattaattta tgattattga
     1621 ttaattatcg gcaacaaaca tcaaacatcg aaaacatgga aaaaataatg tcaacaattt
     1681 ttgccaaatc gggcttggca tcagaaaaaa ataggtttat attcccacct acaaatttgt
     1741 tttcccatta gtacactatc aaccaaaagg agtatccgaa tgactgacct gaacaccctg
     1801 tttgccaacc tcaaacaacg caatcccaat caggagccgt tccatcaggc ggttgaagaa
     1861 gtcttcatga gtctcgatcc gtttttggca aaaaatccga aatacaccca gcaaagcctg
     1921 ctggaacgca tcgtcgaacc cgaacgcgtc gtcatgttcc gcgtaacctg gcaggacgat
     1981 aaagggcaag tccaagtcaa ccggggctac cgcgtgcaaa tgagttccgc catcggtcct
     2041 tacaaaggcg gcctgcgctt ccatccgacc gtcgatttgg gcgtattgaa attcctcgct
     2101 tttgaacaag tgttcaaaaa cgccttgacc accctgccta tgggcggcgg caaaggcggt
     2161 tccgacttcg accccaaagg caaatccgat gccgaagtaa tgcgcttctg ccaagccttt
     2221 atgaccgaac tctaccgcca catcggcgcg gacaccgacg ttccggccgg cgacatcggc
     2281 gtaggcggac gcgaaatcgg ctatctgttc ggacaataca aaaaaatccg caacgagttt
     2341 tcttccgtcc tgaccggcaa aggtttggaa tggggtggca gcctcatccg tcccgaagcg
     2401 accggctacg gctgcgtcta tttcgcccaa gcgatgctgc aaacccgcaa cgatagtttt
     2461 gaaggcaaac gcgtcctgat ttccggttcc ggcaacgtgg cgcaatacgc cgccgaaaaa
     2521 gccatccaac tgggcgcgaa agtgctgacc gtttccgact ccaacggctt cgtcctcttc
     2581 cccgacagcg gtatgaccga agcgcaactc gccgccttga tcgaattgaa agaagtccgc
     2641 cgcgaacgcg ttgccaccta cgccaaagag caaggtctgc aatactttga aaaccaaaaa
     2701 ccgtggggcg tcgccgccga aatcgccctg ccctgcgcga cccagaacga attggacgaa
     2761 gaagccgcca aaaccctgtt ggcaaacggc tgctacgtcg ttgccgaagg tgcgaatatg
     2821 ccgtcgactt tgggcgcggt cgagcaattt atcaaagccg gcatcctcta cgccccggga
     2881 aaagcctcca atgccggcgg cgtagcaact tcaggtttgg aaatgagcca aaacgccatc
     2941 cgcctgtctt ggactcgtga agaagtcgac caacgcctgt tcggcatcat gcaaagcatc
     3001 cacgaatcct gtctgaaata cggcaaagtc ggcgacacag taaactacgt caatggtgcg
     3061 aacattgccg gtttcgtcaa agttgccgat gcgatgctgg cgcaaggctt ctaagcaaac
     3121 gccgccgctc cgcaaacaaa atgccgtccg aaccgcaaat gctgttcaga cggcatttca
     3181 ttatccgccc gttcgaatcg ggtgatacta tttgtatcca gaattgttgg atagtttggg
     3241 agtggagagg aagaaatgat tatggaccaa tactatatgg agttaaagaa taagttatct
     3301 aacagaccaa tcctactgga taatactaat gattttcttt ttgttttagt aaatacagtg
     3361 aaagctatga tagaaaatac tgataaaagt cagctttcag agttagataa aattttagac
     3421 ggtgtcacaa gtcaagaatt gaaacttgcc tatgattttt gtcaagggaa attcggtcaa
     3481 gcaggttttt cttatcgtag gcacccgaat tatttttatt tgtccagttt aattgcgact
     3541 tttccagaat ttgagttgtc taaagcagac cgagattacc tcaaaggaat cataaatttt
     3601 gataactacc ttttatatga gttagattag tctttaatca cctcaaaacc ctatttattc
     3661 aaaaggagaa aaaatgattg agctgaaatt agtagatgag agcagttttc aggcagtgct
     3721 ggatttgaaa atatctgaag ctgatgaacg agcacgtttt gtggctccaa atgtacgctc
     3781 tttagctgac gcatggctct accgggaaaa tgaagatgtg tttccgaggg caatctattg
     3841 ggataagcaa gtggttggct ttctcctgct ggaaatagac aaggatgaag cagaatactt
     3901 tatctggcgg ataatgattg gtcagcagtg
//
LOCUS       DAWFAN010000163         3893 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWFAN010000163 DAWFAN010000000
VERSION     DAWFAN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.163"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            150..653
                     /locus_tag="WGO65_001770"
     CDS             150..653
                     /locus_tag="WGO65_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ0328031.1"
                     /translation="MSDEFNRFIERVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            762..1094
                     /locus_tag="WGO65_001771"
     CDS             762..1094
                     /locus_tag="WGO65_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328032.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            1091..1501
                     /locus_tag="WGO65_001772"
     CDS             1091..1501
                     /locus_tag="WGO65_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ0328033.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            1506..2255
                     /locus_tag="WGO65_001773"
     CDS             1506..2255
                     /locus_tag="WGO65_001773"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0328034.1"
                     /translation="MPSVELLLEIVGLPRGTFYYQLAVQSAEDKYADLKRHIHDIYRR
                     HKGRYGYRRIAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEI
                     LKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWF
                     KFNPLYNNDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(2574..2936)
                     /gene="ssrA"
                     /locus_tag="WGO65_001774"
     tmRNA           complement(2574..2936)
                     /gene="ssrA"
                     /locus_tag="WGO65_001774"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2999..3604)
                     /locus_tag="WGO65_001775"
     CDS             complement(2999..3604)
                     /locus_tag="WGO65_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ0328035.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT          986 a          906 c         1020 g          981 t
ORIGIN      
        1 attaaaagcc gaaaagaaga gtttaaaaaa ttaatggaaa agaagaaacc caaaatctag
       61 aacgagtttt aacccccttt aaaatcccat tcagacgacc ttttctaaaa tccctgtatt
      121 gatttttaat tcaatacagg gatttttcca tgtcagacga attcaaccga tttatcgaac
      181 gcgtcctctc ccacgagggc ggttacgtca atcatcccaa agaccccggc ggcgaaacca
      241 attggggcat cactaagcgc accgcacagg caaacggcta caacggctcc atgcgcgcca
      301 tgacgcgtga acaggcaatc ggcatttacc gtaaagcgtt ttgggagcgt taccgcgccg
      361 accaaatgcc cgaagcggtc gcttttcaat tttttgatgc ctgcgtcaac cacggttacg
      421 gcaatgccgc ccgtatgctc caacgcgccg caggcgtgcc ggacgatgga atcatcggcg
      481 aaatcagcct caaagccatc aattcccttc ccgaaaacga ccttttattg cggttcaacg
      541 ccgagcgtct ggtcttttat accaagctgg gtacgttcac ctctttcggc aagggctggg
      601 tacgccgtgt ggcgcaaaac ctgattcacg ccgccgcaga caatgccgat tgaggagcaa
      661 accatgtcaa aaaagtccat tctcgccctg atggccgcag ccatgcagcc cgatttcagc
      721 cacagcgacc taggcattcg ctacgccatg ccgactcagg gatgttggac gcaagcccac
      781 cgcaagagcg gggtagccgc cgcgaaacgc gcagccaaaa aaaagcgtca caaataaccg
      841 cctttttccg atggctgggc ggcttggtct ctaatccggc cacagggaaa atcagccata
      901 ccaaactttg ggcaaacgtc gccgcagccg ccatgacttg gaagttcgtg caggcggcgg
      961 acgcgcccga atggctttgg tgggcatacg gcgcgatggt cggcgggtat gcattaatca
     1021 aacgcggcat cgccgtcgta ccgcagttgg cggaagtcaa aaaatccgca aatcaggaag
     1081 gggggcggca atgattgaat ttgtccgagc caaaaaacgg ctgctttggg catttgtgct
     1141 tttgcttgtg tggacgtgcg gttaccgata cgccgccgac aaggccgaag cggaacaaac
     1201 cgccctgatt gccacctatc ggcattcttc tatggttgcg gcggaacaat atgccttgca
     1261 gcttaaaaaa gcgcaggacg aaaggcagcg gtggtacgac ttttcccaaa aacaaggaag
     1321 aaagcccgtg aaaaaacagt atccgccgca aacgaaaaaa gccggctatc tgaaaaccaa
     1381 ggaagaactg cttgcggaat tggcttgcct taaagcggaa atggttgccc taaaaaagcc
     1441 cgatgcctta atccatggga aagaagtgcg gcagaaagaa cgcaactcgt cgcagggtta
     1501 aggcaatgcc atccgttgaa ctgctgttgg agattgtcgg actgccacgc ggcaccttct
     1561 attaccaatt ggccgtccaa tcggcagaag acaaatatgc cgatttgaaa cggcatatcc
     1621 atgatattta tcgacgacat aagggaagat acggctaccg gaggattgcg gcagccatcc
     1681 gtcacgcagg aacaccggtc aatcacaaga aagtcagccg tctgatggcg aagacggggc
     1741 tgaaggcagt gatacggcgg cgcaaatacc gctcgttcaa aggagaagtc ggcaaaattg
     1801 cgccgaatat cctgcgacgc tgtttccatg cagaaaagcc gaatgagaaa tgggtaacgg
     1861 acgttaccga gttcaatgta ggcggagaaa agatatacct ttctccgatt atggatttgt
     1921 ttaacgggga aatcgtcagt taccgtattc aaatccgccc gactttcgat ttggccggcg
     1981 agatactgaa aggtgcgccg gagaaaccgg gatcgtctga aaagccgata ctgcattcgg
     2041 atcaaggttg gcaatatcag atgttttttt atagtggatt aaatttaaat caggacaagg
     2101 cgatgaagcc gcagacagta caaatagtac ggcaaggcga ggcaacgccg tactggttta
     2161 aatttaatcc actatacaac aacgatagaa tcaagttgaa attaaaaggg ctgagccttg
     2221 ttcagtacag aattcagtcc ctgaaagccg cttgattaaa ctgtccgact ttttggggtc
     2281 agttcggctt cggcattttt ttatccgttt gggggtaact tgtttggaaa gctgcaagct
     2341 tataaataaa ggattacatt taagttttgg gtaacctttt taaaaaaatg cgtgatgact
     2401 tttgcatttt taaggcgttt tttggggtaa ttcgtgaaaa gttaccccaa aagttacccc
     2461 ataaatggcg aaaactcaag catacgccag catattcaag gcaacaaaaa agccttgaaa
     2521 ctgttgaagt tcaaggcttt tttgtgttgc aggatgctgc tgaaaatagg gtatggtgga
     2581 ggcgggggga atcgaacccc cgtccgaaag tcctctacaa agcgttctac atacttagtt
     2641 gtgtctattt gaaaatctta tttccatcat gccgaccaac aggccttttg gaaaccagtt
     2701 accttaaatc tcatttcctg ccaagtaacc cggcaggaaa ccagtcaatg taagatgacg
     2761 ttgcggtggc tttcgccaca cagcccattg accgactgct gcaacggcta gccttaagcg
     2821 gctaaagcgt aagtttcgtc gtttgcgact atttgaattc agtgttttac gggaatctga
     2881 gaccccggta tgcccgcatc tgcttcgcaa cccccgtcga aaccaaggtc gcccccagaa
     2941 atggtttgca aattatacgg atattgtgcg gtgctgccaa gtctgtcgga gaaatttgtc
     3001 agtcttgctg ccttaatttg cgtttgagca ggatgcggac gcagccgtcg ttgccttccc
     3061 ggggttcggc gtaggcgagt acatcggggt gttgcatcag ccagtttcgg gtcatatttt
     3121 tcagaacggg tttgtagcct ttggaaccta atccgctgcc gtggatgatt tcgccgcata
     3181 cgccgcgttt ttgggtgaat gcgatgaatt cgttgaggac tttttgggct tcttcctgtg
     3241 tgtagccgtg caggtcgaca tcggtaacga cgggatagta tccgtttttc aggcgttgga
     3301 tgtcgttttt tccctgtccg tttttgctga agctggcggg cgggtcgttg tatgtgctgc
     3361 cgatgtagaa gtagttttct tcgtcggcgc ggttgtcttt gggacggact ttgatggggg
     3421 ttttgtcggg cggcgcataa tattgctgcc ggttttttaa tggggagact tgtccgactg
     3481 cttgtgaaaa atcgaaatcc tgttcttgtt tttgcttgtt tttttcggct gcctgttttt
     3541 ctgccgcttc tttttgggct tgtttgccca gttgtttgag gatgttttgg aagtcggtat
     3601 tcatattttt tcctgttatt tgtccgatgg ctgtttcggg cggggtttta atttgccgga
     3661 atgtttgcca atcgggggag gatgattttg ttgcctgcgt atgttttttg aaagtgtgat
     3721 tgtatatcaa aaagaaatgc ggcaaccgtc ggcagtgttg attgccggaa atgcggaccg
     3781 gtcgaaccga tatgcccgaa cgcctgataa agttttaaaa acctgccttg cgaagcaggc
     3841 tgacgtgttt tgccaatctt gaattgccgg aaacgcgaaa cacggaaatc tga
//
LOCUS       DAWFAN010000164         3886 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWFAN010000164 DAWFAN010000000
VERSION     DAWFAN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.164"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..2118
                     /locus_tag="WGO65_001776"
     CDS             <1..2118
                     /locus_tag="WGO65_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0328036.1"
                     /translation="GKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFGG
                     ESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTAGVQVGSAQLIKGASTAVNGMDPE
                     GAVSGSVNIETKKAADEGNRKIGLGRFSNNRAQGTFDLGQRFGENKEFGVRANGKLRH
                     GDTPRHGYSEDNKEFALNADYRGETLRVAFDSIYAKRKTNGGRARIQDIQNAGGRLFG
                     APDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVCL
                     NATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLTARGEFETGPVTHN
                     WSMAFDRVIRQRKTINGSGNGNSKIEVKANENIANQLASFTADYPNSWENSANLDANI
                     KVNSLALSDTLGFVDNKYRLTLGERFQTVEYTDKKSQSGDAKRFSPMLMAAWVPQPDL
                     VVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKRPG
                     YWRGHTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANLLN
                     NTLRPTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLNGN
                     VSYFGKSYQDTQKQYAFPSYTLIDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQRG
                     QYDRSFAVVGLPRTYWLKAELDF"
     gene            2242..3792
                     /locus_tag="WGO65_001777"
     CDS             2242..3792
                     /locus_tag="WGO65_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ0328037.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         1081 a          969 c          987 g          849 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcaccgatt accgtcgtca attacgacga acaagccctc
       61 aataacaccg aagcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gcgaaagcaa cacgctgacc ggcctgtatt tccgcggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggcttggcc ggtatgtacg gcacgcaagg caccgccggc
      241 gtgcaagtcg gctccgcgca actgattaaa ggcgcgtcca ccgccgtaaa cggtatggac
      301 cccgaaggcg cggtctccgg ttccgtcaat atcgaaacca aaaaagccgc cgatgaaggc
      361 aaccgcaaaa tcggtttggg ccggtttagc aacaaccgcg cccaaggtac gttcgacttg
      421 ggtcaacgct tcggcgaaaa caaagaattc ggcgtgcgcg ccaacggcaa actgcgccac
      481 ggcgacactc cgcgccacgg ttacagcgag gacaacaaag aatttgcctt gaatgccgac
      541 tatcgcggcg aaacactgcg cgtggcgttc gactccatct acgcgaaacg caaaaccaac
      601 ggcggccgtg cgcgtattca ggatattcaa aacgccggcg gacgcttgtt tggcgcgccc
      661 gatggtaaaa ccaacctgct gccttcttgg aactggcaaa ataccgccgg ccaaaccaat
      721 atgcttacgt ttgaatggga tgcgtttgat aatgcacaga ttacaggcgg tatcggctac
      781 aacaaggcac gctattacgg cacgctgatt tccccgaccg tatgtttgaa tgctaccagc
      841 acttgtaccg atatgcaaaa cacaaacgcc agcggcaaag taacggcacg caactatgac
      901 tacaataccg gtactgcgcg cctgaccgac caatatttcc gcacattgag tatgaacctg
      961 accgcacgcg gcgaatttga aaccggcccg gtaacacata actggagtat ggctttcgac
     1021 cgcgttatcc gccagcggaa aaccataaac ggcagcggga acggcaacag caagatagaa
     1081 gtgaaggcaa acgaaaatat tgcgaatcaa ttggcatcgt ttacagccga ctatcccaat
     1141 tcttgggaga actctgccaa cttggatgcc aatatcaaag tcaacagcct ggctttgtcc
     1201 gacaccttgg gcttcgtgga taacaaatac cgcctgactt tgggcgaacg tttccaaaca
     1261 gtcgaatata ccgacaaaaa atcacagagc ggcgatgcca aacgtttcag cccgatgctg
     1321 atggcggcgt gggtgccgca acctgatttg gttgtgtacg gcaactatat ggaagacttg
     1381 gagcctgccg acatcaaaac cgatgattcc ggcgaaacga caatggcgaa accgcgtgtc
     1441 agccgccagt ttgaagtggg cgtgcgtaaa aactggggca atttcgtaac cacgttaaac
     1501 gcgttccaaa tcaaacgccc gggctactgg cgcggtcata caactaccaa gacaacaaaa
     1561 ggggtaacaa caacgctgac ctatggcaac aattccgatt tcgcccgcta taaggcgcaa
     1621 ggcggcgcgg ccggcgacga gcaaggtatg gaacgcaatc gcggtattga gttcaacacc
     1681 tacgcgaact tgctcaacaa tactttgcgt ccgactttgg gtctgatgta tctgcaatcg
     1741 accgtgaaag agtatccaaa ttcacgcgat atgctggtta acggcgtaca agtcgccaat
     1801 ccgcgcgtaa ttgccaaagc aggtttggaa tgggatacac cgtttgccaa aggattgact
     1861 ttaaacggta atgtttcgta tttcggcaag tcttaccaag acacgcaaaa acaatacgcc
     1921 ttcccgtcct acaccttgat tgacgtaggc gcgcgctaca aaaccaagct cggcaaaaat
     1981 actttgaccg tcagcagctc ggtggaaaat ctgttcaaca aaaactactg gcaggtacag
     2041 cgcggccaat acgaccgcag cttcgccgtc gtcggtctgc cgcgtactta ctggctgaag
     2101 gcggaattgg atttctgatt tctccgcatc tggaaaatgc cgtccgaaca ggtttcagac
     2161 ggcatttctt ttaaagagat atggcgggat acgcttaaat aatgctattc tagcgtcttt
     2221 gaaacctgct tgtttgaagt gatgaaaaaa cctttgattt cggttgcggc agcattgctc
     2281 ggcgttgctt tgggcacgcc ttattatttg ggtgtcaaag ccgaagaaag cttgacgcag
     2341 cagcaaaaaa tattgcagga agcgggcttc ttgaccgtcg aatcgcacca atatgagcgc
     2401 ggctggttta cctctacgga aacgacggtc atccgcttga aaccggagtt gctgaataat
     2461 gcccggaaat acctgccgga caacctgaaa acagtgttgg aacagccggt tacgctggta
     2521 aaccatatca cgcacggccc tttcgccggc ggattcggca cgcaggcgta cattgaaacc
     2581 gagttcaaat acgcgcctga aacggaaaaa gttctggaac gcttttttgg aaaacaagtc
     2641 ccggtttccc ttgccaatac cgtttatttt aacggcagcg gtaaaatgga agtcagtgtt
     2701 cccgccttcg attatgaaga gctgtcgggc atcaggctgc actgggaagg attgacggga
     2761 aaaacggttt atcaaaaagg tttcaaaagc taccgcaaca gctatgatgc gcccttgttc
     2821 aaaatcaagc tggcagacaa aggcgatgcc gcgtttgaaa aagcgcattt cgattcggaa
     2881 acttcagacg gtatcaatcc gcttgctttg ggcagcagca atctgacctt ggaaaaattc
     2941 tccctagaat ggaaagaggg tgtcgattac aacgtcaagt taaacgaact ggtcaatctt
     3001 gttaccgatt tgcagattgg cgcgtttatc aatcccaacg gcagcatcgc accttccaaa
     3061 atcgaagtcg gcaaactggc tttttcaacc aagaccgggg aatcaggcgc gtttatcgac
     3121 agtgaagggc agttccgttt tggcacactg gtttacggcg atgaaaaata cggcccgctg
     3181 gatattcata tcgctgccga acacctcgat gcttctgcct taaccgtatt gaaacgcaag
     3241 tttgcacaaa tttctgccaa aaaaatgact gaggaacaaa tccgcaatga tttgattgcc
     3301 gccgtcaaag gcgaggcttc tggactgttc accaacaatc ccgtattgga cattaaaact
     3361 tttcgtttca cgctgccgtc aggaaaaatc gatgtgggcg gaaaaatcat gtttaaagat
     3421 ttgaagaagg aagatttgaa ccaattgggt ttgatgctga agaaaaccga ggcagacatc
     3481 agaatgagta ttcctcaaaa aatgttggaa gacttggcgg tcagtcaggc aggcaatatt
     3541 ttcagcgtca atgccgaaga tgaggcggaa ggcagggcaa gtcttgacga catcaacgag
     3601 accttgcgcc tgatggtgga cagtacggtt cagagtatgg caagggaaaa atatctgact
     3661 ttgaacggcg accagattga tactgccatt tctctgaaaa acaatcagtt gaaattgaac
     3721 ggtaaaacgt tgcaaaacga accggagccg gattttgatg aaggcggtat ggtttcagag
     3781 ccgcagcagt aatgggtcaa atgccgtctg aaatgttcag gcggcatttt gttgtattgg
     3841 aaactgccgt tttcaaccat tcctttagat aagccgtgtc ggattc
//
LOCUS       DAWFAN010000165         3859 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWFAN010000165 DAWFAN010000000
VERSION     DAWFAN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.165"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WGO65_001778"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WGO65_001778"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ0328038.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WGO65_001779"
     CDS             1439..1924
                     /locus_tag="WGO65_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ0328039.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGO65_001780"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGO65_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ0328040.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          783 a          941 c         1078 g         1057 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaga tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggct ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgcgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcgaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccaac ggtcgtgcga
     2161 aatcaccatc acgctaccgg caaattccag caatgcgtct tccagcgcgc gcagggtttc
     2221 tacgtcaagg tcattggacg gttcgtccaa cagcaataca ttgccgccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAWFAN010000166         3763 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWFAN010000166 DAWFAN010000000
VERSION     DAWFAN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.166"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..414)
                     /locus_tag="WGO65_001781"
     CDS             complement(<1..414)
                     /locus_tag="WGO65_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ0328041.1"
                     /translation="MTHISSPRNEFIRGIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNFAGGSEFATVNLWAESLPILLIATVTFMINSRHILMGAALALHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
     gene            559..1011
                     /gene="dut"
                     /locus_tag="WGO65_001782"
     CDS             559..1011
                     /gene="dut"
                     /locus_tag="WGO65_001782"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ0328042.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGSH"
     gene            1089..2276
                     /gene="dapC"
                     /locus_tag="WGO65_001783"
     CDS             1089..2276
                     /gene="dapC"
                     /locus_tag="WGO65_001783"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ0328043.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDFQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLKRGREKLVMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            2538..3317
                     /gene="yaaA"
                     /locus_tag="WGO65_001784"
     CDS             2538..3317
                     /gene="yaaA"
                     /locus_tag="WGO65_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ0328044.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3400..3490
                     /locus_tag="WGO65_001785"
     tRNA            3400..3490
                     /locus_tag="WGO65_001785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3434..3436,aa:Ser,seq:tga)
     gene            3568..3657
                     /locus_tag="WGO65_001786"
     tRNA            3568..3657
                     /locus_tag="WGO65_001786"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3602..3604,aa:Ser,seq:cga)
BASE COUNT          987 a          884 c          921 g          971 t
ORIGIN      
        1 aggcatattg aatgcgggca aaccggctgc tttccgtttt tggatttcgg agaatgccat
       61 cgcccagctt tcatcacaca taaaaaacag tgcgggcacg gcttttttca gcggtatttc
      121 tttcaggtgc agggcaagtg ccgcccccat caggatatgc cgcgaattaa tcataaaggt
      181 tacggtggcg ataagcagta tcggcagaga ttccgcccac aggttgaccg tggcaaactc
      241 ggagccgccg gcgaagttca tactggtcat caacaacatt tccagccagc tcatgccttt
      301 ttgtccgccc tgcataccga gtattaatgc ccaaggcaga agtccgataa gtatgggcga
      361 actttctttg atgccgcgta taaattcgtt acggggcgag cttatgtgtg tcatattata
      421 atattgatat gtaagaaggt cgggcattat acacagacgg agtatttcaa aacaaaaccg
      481 atttgccgtg tttcagcgta aacacggctt gtgtataatc tcccatcttt aaaaccggcc
      541 gtatgcagga gcaagatgat gaatattgaa gtagaaatga aagtgttgga cgagaggatg
      601 gcggatttta tccctgccta tgcgacggag ggttctgctg gtttagattt gcgtgcctgt
      661 ttggatgagg aagttgtctt gcagccgggt gaaacgtttc ttgtaccgac aggtttggca
      721 atttatttgg cgaatcccgc atatgccgcc gttttgctgc cccgttccgg attggggcat
      781 aaacacggca ttgttttggg caatttggtc ggcttgattg actccgatta tcaaggggaa
      841 ttgaaggttt cgttatggaa cagaagcagc gagccgttta ctgtcaaacc gtttgagcgt
      901 atcgcgcaga tggttatcgt gccaatcgtg caggcgcgtt tcaaacgtgt cgaggaattt
      961 gtcggaagca gccggggaga aggcggcttc ggcagtacgg gttctcacta aaaatataga
     1021 atgccgtctg aaagacacgt caggttcaga cggcatatct tccgatatac cgacaaccgg
     1081 agaaaatcat gaatacctta ctcaaccaac tcaaacccta tccctttgcc cgactgcgtg
     1141 aagcgatgca gggcatttcc gcccccgaag gtctggaagc cgtcccactg catatcggcg
     1201 aaccgaaaca tccgacaccg aaagtcatta cggatgcgct gaccgcctca ttgcacgagt
     1261 tggaaaaata tccgctgacg gccggtttgc ctgaactgcg tcaggcgtgt gcgaactggt
     1321 taaaacgccg ttatgatggg ttgacgctga attccgacac cgaaattctg ccggttttgg
     1381 gaagtaggga ggcgttgttt tcttttgtcc aaaccgtgtt gaaccctgtt tcagacggca
     1441 tcaaacccgc aattgtcagc ccgaatccct tttatcagat ttacgaaggt gcgacacttt
     1501 tgggcggcgg tgaaatccat tttgccaatt gccccgcgcc gtctttcaac cccgattggc
     1561 gtagtatttc cgaagaggtt tggaaacgca ccaaactggt gttcgtctgc tcgcccaaca
     1621 accccagcgg cagcgtgctg gatttggacg gctggaaaga agtttttgat tttcaagaca
     1681 aatatggttt cattattgcc tcggatgaat gctattccga aatctatttc gacggtaaca
     1741 aacctttagg atgtctgcaa gctgcagcgc aattgaaacg gggcagggaa aaactggtta
     1801 tgtttaccag tttgtccaag cggtccaatg ttccaggcct gcgttccggt tttgtcgccg
     1861 gcgatgccga actgcttaaa aattttctgc tttacagaac ctatcacggc agtgcaatga
     1921 gcattcccgt gcagcgcgca agcattgccg cttgggatga cgaacagcac gttatcgaca
     1981 accgccgcct gtatcaggaa aaatttgagc gcgttattcc cattttgcaa caggtatttg
     2041 acgttaaatt accggatgcc tcgttttaca tctggttgaa agtccccgat ggcgacgatt
     2101 tggcatttgc acgcaattta tggcaaaaag ccgctatcca agtattgccc ggacgctttt
     2161 tggcacggga taccgaacag ggcaatcccg gggaaggtta tgtccgtatc gctttggttg
     2221 ccgatgtcgc aacttgtgtc aaagctgcgg aagacatagt ttcactgtat cggtaaggaa
     2281 tcggaaatgc cgtctgaact tttgttcaga cggcattttt caatattcta cggttgaatt
     2341 tgctgtaacg gtatttatag tggattaaca aaaatcagga caaggcgacg aagccgaaga
     2401 cagtacagat agtacggcaa ggcgaggcaa cgctgtactg gtttaaattt aatccactat
     2461 acttcacttt taatcggctt gcccgcaaac acgtttaaac ttaaaatccc cgtgtttgac
     2521 acaataccga gcagattatg ttttttgtcc tttcccctgc gaagaacctt aatgaaaaag
     2581 accctgcccc tgtcagcgag tttacccaac ccgacctgct ggcagagtcc gacattctaa
     2641 tgcagcagtt gcgcgagctt gcgccgcaac agattgccga actgatgcac gtttccgaca
     2701 aaattgccct cttaaacgcg cagcgcaatg cagaatggaa cacgccgttt acgccggaaa
     2761 acgccaaaca ggcggtcttt atgttcaacg gcgatgttta cgaaggtatg gatgcaaaca
     2821 cattggatat tggacagata cgctatctgc aaaaccatgt ccgcctgctg tccggtctgt
     2881 acggtcttct tcgcccgtta gacctgatac agccctaccg tttggaaatg gggacggcat
     2941 ttgccaattt gcgcggcaag aatttgtatg agttttgggg cgacatcatt accaaccttt
     3001 taaatgacac gcttgcccaa gcaggcagca atacgcttgt caaccttgcc tcacaggaat
     3061 atttcaagtc cgtcaacacg aaaaaacttc gggcgcggct gattaccccg atatttaaag
     3121 acgaaaaaaa cggtaaatat aaaatcatca gtttctatgc caagcgtgcg cgtggattaa
     3181 tggtgcgcta tgcggcagaa catcatatta ccgaccctga aatgctgaaa aattttaatt
     3241 acgaaggcta cgcattcaat gacgcggctt caaatgaaag cgaatgggtt tttatgcgtt
     3301 cggaacaaat aaagtgaaaa caataaatta agtattttcc gaaaaaagtg cttggcaaaa
     3361 tgtataaatt tcattattat tcctaatctt caagaagacg gaagcgtggc agagcggttt
     3421 aatgcaacgg tcttgaaaac cgtcgagggt tgatagccct ccgtgagttc gaatctcacc
     3481 gcttccgcca atccttgagg tcaaaaacca aataaaaatg aagtagcata aagcatcggc
     3541 atttttattt ggttttactg cattatcgga aacgtggcag agaggctgaa tgcagcggac
     3601 tcgaaatccg ctgagggtgc aaatcctccg tgggttcgaa tcccaccgtt tccgccacaa
     3661 aacaaaaccg ccctgattcg gggcggttct tttttgttca agttgagacc tttgcaaaat
     3721 tcctttccct cccgacagcc gaaacccaaa cacaggtttt cgg
//
LOCUS       DAWFAN010000167         3751 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWFAN010000167 DAWFAN010000000
VERSION     DAWFAN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.167"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(214..1278)
                     /locus_tag="WGO65_001787"
     CDS             complement(214..1278)
                     /locus_tag="WGO65_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0328045.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRENRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIIH
                     NGIDTDRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTENIASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAAH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="WGO65_001788"
     CDS             complement(1279..2037)
                     /locus_tag="WGO65_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0328046.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFQRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="WGO65_001789"
     tRNA            complement(2112..2187)
                     /locus_tag="WGO65_001789"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..3377)
                     /locus_tag="WGO65_001790"
                     /pseudo
     CDS             complement(2241..3377)
                     /locus_tag="WGO65_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            complement(3322..>3751)
                     /locus_tag="WGO65_001791"
     CDS             complement(3322..>3751)
                     /locus_tag="WGO65_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0328047.1"
                     /translation="LAADEVFEDPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNG
                     RVYTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSSFIHYRINHSKEFAD
                     RQNHINGIENFWNQAKRVLRKYSGLARTFPRIGSATGCAD"
BASE COUNT          854 a          922 c          981 g          994 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc agtttatatt gcatccaata atttgtcggc
      241 ggtatggttg atggtaaaac gggcggcgac gcattgatga gctgccgttg ccagtgcgtt
      301 ttttgcttcg gggtttaaga cgaggcgttc gatttcgtcc gccaaagatt caggcgtaag
      361 cctatccagc agaatccccg attgatgatg ttcgacaatt tccttttgcg cccccaaagt
      421 attggaaatc accgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caatgttttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatcaggat gtccgtgccc tgccaatttg agtctgattt gaggatattt
      661 ccgatgcaat atcacacagg cttcaattag gttttccaat cctttttccg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaccg
      781 atcggtatcg ataccgttat gaataatccg gtatttttct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tctgtgtttt tctgtatcag
      901 gcggtggtaa aaatcggttt tgttggcgac gacattatgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggttttccc ggataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat tgtgcagccc cgaagcccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccacc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccagatttg tacagataat atagtttgac
     1321 atatttaatc atcgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 aagaaaccct ttgttcagaa tataaatttt gaaaaaaccc caaatcggac ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg agatggaagt
     1501 atatttgttg aacttgttga aatattgttc ccagttgtcg tacgtataat ggtacataaa
     1561 atgtttcaga cggcgtttgg ggtagggggt ttgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgtct ttcttcggca tcaaacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ctcgacaaaa taggcggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgttgga agacttttgc acccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcgtcga taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcccgaatg tggtttgctt cgtttttggc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg attaagacgc agttgcttcc cccgaaggca
     2281 aacgatgagc tggcggcaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gcaggttggg gtcgttctgc ccgtcccaaa gccggggcgg aagttttcct
     2401 tcgggattgc tttgccggtt ggcaatgccc cacgcgaacg cggcttcgat tgcgtccgcc
     2461 gcgcccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcgcggct ttccatattg tcgttgtggt gcgtcccggt gccgtgcaga
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggca
     2641 aggattgcgc cttacgcgtc ggggcggggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattttc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgggaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagcccg aaaaagatgc cgatattggc ggcggcggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacatgcc ggtcagcaac
     3121 aattcgccta catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atgcgtccgt cagtaatgct ttcgtctccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacacgc cccatacttc tgagctgctt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatc ctcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgtgcc aatccactat attttcgcaa gacgcgtttt gcctgattcc aaaagttctc
     3421 aatgccgtta atgtggtttt gacggtctgc aaattccttg gaatggttga tgcggtaatg
     3481 gataaaactg ctcacgtcca acttgtcgca gctgctcaga ctatcggtat aaacaatact
     3541 gtccggcatg attttctttt tgatgacagg gagtaacgtt tcagacttgg cattgtttac
     3601 gacaacggta tagacccgtc cgttgcgttt cagaatgccg aaaacaacca cttttcctgc
     3661 cgcaccgcga ccacgtctgc ctttacgccg tccgccgaaa tcgctttcgt ccggctcgac
     3721 aggatcctca aaaacctcat cggcagccaa g
//
LOCUS       DAWFAN010000168         3747 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWFAN010000168 DAWFAN010000000
VERSION     DAWFAN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.168"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(278..2710)
                     /gene="hupB"
                     /locus_tag="WGO65_001792"
     CDS             complement(278..2710)
                     /gene="hupB"
                     /locus_tag="WGO65_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ0328048.1"
                     /translation="MPIPFKPVLAVAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVNIKNAGYIYDPTDTNGPSKWLDYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTLTSDATGDYRHRQDVSYRRRTGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINSEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFVKLYDPKIFTSNQAKITMLIESR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDPNKPTYAPISDTTALVSSP
                     VWQNQNRSTAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVNNHTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(2741..>3747)
                     /locus_tag="WGO65_001793"
     CDS             complement(2741..>3747)
                     /locus_tag="WGO65_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein"
                     /protein_id="HEZ0328049.1"
                     /translation="PHVPVSIPTATPLPKGEVTLSSDNGNIENINTANTETHPPRRTR
                     RSLYASPQNTSSGISIQQREVEKIYFGVKSPEKSFIFQTPGGAQYALSSYADPIVPSY
                     SSPDFKIPDRHAGQRLADGSRIFICCSDSGATSYAEITKQDYMKFGAWIGPNGEIDLF
                     AGGFPVGKTPPPAFSYGNSTPETALSKGKITYQVWGIRVRNRQFVTSSYTPPKSGSYY
                     GTLANTPVLSFITANFNSNKLAGEIRGNSDYGPSVKIENATINSLSFSGTATSGGKTG
                     NLEGKFFGKFNGYRDTETSIGGKITFDGDRSLDTVFGGVSYKKELENNTDTSTTHLTK
                     "
BASE COUNT          691 a          856 c         1189 g         1011 t
ORIGIN      
        1 gggaatgacg tggtgcaggt ttctgtgcgg atagcttcgt cattcccgcg caggcgggaa
       61 tccaggttcg ttgagtttca gtcatttccg ataaattctt gcagctttgc gtttctagat
      121 tcccacttcc gtgggaatga cggagagtcg cgggaatgac ggagagttgc gggattgggc
      181 ttggtacaag cctgaaaggg ggcggcggtg cgactgtaaa aaatgccgtc tgaaacccaa
      241 atcatcgggt ttcagacggc attggcacat caggagctta gaacttcgct tcgatggtga
      301 aattgtagct cctgcccggc gaggtgaaac gttggatgcc ggcgtgggtg tggttgttga
      361 cgcggttgac cgtgccgaac tcgcggatgc tgcgtaaaga ttcccaagtg tagtactgct
      421 tgttggtaat gttgtacgcg gcggcgcgga gcgtaacctg tttgccgatg ttgaggtagg
      481 cggaaaggtc gaacagcgta taggccttgc tgtgtttggc ataaggccat gggttgttca
      541 agtcgtcgtt gctgtggacg gtgtcggacg gctttttggc ggcggtgcgc gtggcgtagg
      601 cgttgatgcc ccagcgtttg gaaggcgcgt catagcccag gctgtaaacc gccgtccacg
      661 gcgaaagcgc gttgatgggc gtttctttgc cgttgttttg cgttgccttg cccttgatgt
      721 agctgacgtt gaggccggtg tgcagccctt tgggcagccc gatgctgtcg aggttccacg
      781 tgccgttaaa ctctatgcct ttcacccagg cggtggagcg gttttggttt tgccaaacgg
      841 gcgagctgac caatgccgtt gtgtctgaaa tcggggcgta ggtgggtttg ttagggtcgt
      901 ctgacgaaac gcccatatac gtcaattcga taaagtcgcg gtatttggtt ttgaagcccg
      961 agagcttgaa gttgcccgct ttgccgctgc ccgccaggcc caattcccag tttttggctt
     1021 tttcggcttt caggtttggg ttggctttca ggtagaaatc ggggtgtggg aacagcagcc
     1081 aggtttcgtc cgaagtcggt gcgcggaagc cggtgctgta ttttgccaac aagtgcagat
     1141 gcttggtaaa ccgccagtcg aaccccgtgc cgtagctgaa gccggcgtgc gcgcgttccg
     1201 aacccaaatg gggaatctgc cctcggatgg cggtggtgta tttcggatcg tctttcgcgc
     1261 tgctgctgtt tttgtcgtag cgtatgcccg cattcaggcg gaagcggtcg ttgccgccca
     1321 agtgaaacgc attgttccaa taggcaaatt tatatttcga ccggctttcg atcagcatcg
     1381 tgattttggc ttggttggaa gtgaagattt tggggtcgta cagttttacg aaatagctgt
     1441 aatccgagtt ggcattgtcc cctctgccgc cgcccaagcc gtattgcgcc gcccaaacgg
     1501 ctttggaaaa gtcgagttgt ttttcaaaat cggcagtcca ttgcgcggtg ttttggcgga
     1561 tatgccggaa cgaatggtaa acttcactgt tgatgccgcg caaatcgtaa tttttcggga
     1621 tgtcccaagt ccaagtgttc atatcgatgc gctgcttgtc gtagcgcagc ttgaggctgt
     1681 cccacgggcc gtgttccaat tcgtttttgt attcgacgcc ggtgcggcgg cggtagctca
     1741 cgtcttggcg gtggcggtag tcgcccgttg cgtcagatgt gagcgtaccc gtccacaaat
     1801 tagacagctc gttggtaaaa cggtcggtgc gggagtcttc aaaaatccag ccgatgcggt
     1861 tgcggtcgtt gaaattgtag cccagcttga acaaggtgct tttgtttacc cactcttgcg
     1921 ggtcgggttg ggagcgtgca acccctgtgg ctacgtaatc cagccatttg ctgggtccgt
     1981 ttgtatcggt cgggtcatag atatatccgg cgtttttaat gttgacatcg ccctcggtcg
     2041 agcggttttt ggtttctttg ccgaagcggc gggtataaac caataaggca tccaaaccaa
     2101 agagcctgcc ggcggcggtg atgctgctga atttttggct gtttttgccg acgctgccgc
     2161 cctttatccc caaatggtag ggcttgtctt cggaaacata atcgcttgcg gatttggttt
     2221 ggtaattgac tgcgccgccc aatgcgccgc tgccggattt gagcgagtcc gcgcctttgg
     2281 tgatggttac ttcggaaaag ttttccggct cggaagtgtt gcggttggcg ttgaagttgc
     2341 cgtacgcgcc gaacaattct tggaaggctt cggaagagcg gctttccgcc tgcgccagcc
     2401 cgtcaacgtt gatggcgacg cggtctttgt ccacgccgcg tatggtaaag ccgttagaac
     2461 ccgccctgcc gccttcgacg acggaaatgc cggggtcgta gcgcaccagg tcgtgttcgt
     2521 cgttgaccaa gagcttgtct aaggttttgc ggcggatttt ttcttcgccg agtttttggg
     2581 ttttgtgcgt gccggtaacg gtgatttcgt tcagcgtttg ggcggactgc ggcgcggggt
     2641 ctgccgcaaa ggcgggaaac gcttgggcga tggcggcgac agccaatacg ggtttgaagg
     2701 ggatgggcat aagaaaacct ttttaaaagc ggattgatta ttattttgtg agatgggtgg
     2761 tacttgtatc agtgttgttt tcaagttctt ttttataact cacaccgccg aatacggtat
     2821 cgagggagcg gtcgccgtca aaggtgattt tgccgcctat gctggtttca gtgtcccggt
     2881 agccgttgaa ttttccaaaa aacttgcctt ccaaattgcc ggttttcccg ccggacgtgg
     2941 cagtgccgga aaaggataaa ctgttaatcg ttgcgttttc aattttcaca ctcggtccgt
     3001 aatcgctgtt gccgcggatt tcgcctgcca gtttgttgct gttgaaattg gcagtgataa
     3061 aggaaaggac gggggtgttg gcaagtgtcc cataataaga accgcttttc ggcggcgtat
     3121 aagaggaagt aacaaattgc ctgtttctga cgcggatgcc ccaaacctga taagtgattt
     3181 tgcccttcga caatgcggtt tcgggcgtgc tgttgccgta ggagaacgca ggaggtggcg
     3241 ttttgccgac ggggaagccg ccggcgaaga ggtcgatttc gccgttggga cctatccacg
     3301 ccccgaattt catataatct tgtttggtaa tttccgcata cgaagtagcc ccggagtcgc
     3361 tgcagcagat aaagatgcgg ctgccgtcgg caagccgctg ccctgcgtgg cggtcgggaa
     3421 ttttgaaatc gggggaggag tagctgggga cgatggggtc ggcgtaggat gaaagggcgt
     3481 attgcgcgcc gccgggtgtt tggaagatga atgatttttc tggtgattta acaccgaaat
     3541 agattttttc tacttcccgc tgctgaatgg aaataccgga ggatgtgttt tgcggggagg
     3601 catagaggga gcgtctggtg cggcgcggcg gatgtgtttc agtgttggcg gtgttgatgt
     3661 tttcgatatt gccgttatcg cttgataacg ttacctcgcc tttgggcagc ggcgtggcgg
     3721 tggggatgga cacggggacg tgcggtt
//
LOCUS       DAWFAN010000169         3730 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWFAN010000169 DAWFAN010000000
VERSION     DAWFAN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.169"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(228..551)
                     /locus_tag="WGO65_001794"
     CDS             complement(228..551)
                     /locus_tag="WGO65_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ0328050.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(632..1288)
                     /locus_tag="WGO65_001795"
     CDS             complement(632..1288)
                     /locus_tag="WGO65_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ0328051.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     ISDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNIVPDVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRQGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            complement(1391..1894)
                     /locus_tag="WGO65_001796"
     CDS             complement(1391..1894)
                     /locus_tag="WGO65_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ0328052.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEYLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            2078..2851
                     /locus_tag="WGO65_001797"
     CDS             2078..2851
                     /locus_tag="WGO65_001797"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ0328053.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2858..3208
                     /gene="secG"
                     /locus_tag="WGO65_001798"
     CDS             2858..3208
                     /gene="secG"
                     /locus_tag="WGO65_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ0328054.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKYGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3222..3307
                     /locus_tag="WGO65_001799"
     tRNA            3222..3307
                     /locus_tag="WGO65_001799"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3256..3258,aa:Leu,seq:gag)
BASE COUNT         1018 a         1176 c          786 g          750 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaactttcag tttgccagcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg atactgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccaa atacatcgcc gtatgcaggc tgcggatacc gtgatggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgccttca attccgccga cccaagttgc
      541 acaatatcca tcgcaccccc caaaaaaaaa ccaagcaaaa tgccgtctga agccccaaac
      601 ccactttcag acggcatgac ctgtcaacat cttaaaaatc gaaaccgcca aacggatcgg
      661 catccttatc atccaaatgc gccaccaagg tatcgaacag caccttctct tcaaacacat
      721 cgccttgacg cgtaatcaaa agcgcgcgtt gaacaggctt gcggcctacg ataaccacca
      781 tgcgtccgcc atctttcaac tgttctttca acacatcagg cacaatattg accgcaccgc
      841 cgacataaac cgcatcaaac ggcgcacctg cggaaagttc ggtcagcccg ttgttttgca
      901 cataatcgat attgtccaaa cccaagccgt ccaacaccgc tttggcgcgg ttttgctgtt
      961 ccgcatcgat atcgtccgaa attaccctgc ccgccaattt tgccaacagc gcggtcgcat
     1021 agcccgaacc cgtgccgatt tccaaaaccg tatcgttttt cgtcagcttc aagccctgcg
     1081 ccagccgcgc cacgactttc ggctccagca tcttatgacc gttggcaagc ggcagcgcca
     1141 tatccgcata cgccaaaccc tgcaaatcct catcgacaaa aagctcgcgc ggaatctccg
     1201 ccaaagcgtc caaaacgtca aaatccaata catcccaagg acggatttgc tgttcgacca
     1261 tattgaaccg cgctttttca aaatccattt agtgctccgt caaataattg tttcaacaat
     1321 agcaggcatt ataaaaccac actcacgccg cgccaacttc ggcacgcgcc gacagcccgt
     1381 tttgtcagtc tcaagccgcc tgacgcgaag cctcaaaccg cttctccaaa atcttcgcca
     1441 gttcgcccaa atacaaagca tctgtctcat caaactgcgc caaatattcg ctgtccgcgt
     1501 ccaacacgcc gatacagcgg ccgtctgaaa acagcggcac gacaatctcc gaacgcgaca
     1561 aagacgaaca ggcaatatgg tcgggatgcg cgtccacatc cccgacgacc accgtcccgc
     1621 ccttcgccca agcctgaccg cacaccccgc gtccgaacgg aatccgcgta cacgccaaag
     1681 gtccctgaaa cggtgccaaa accaattcgt ccgaacgcgt atcgaccaaa taaaaaccca
     1741 cccaaaacca gccgaacgcc tccttcaaaa ccgccgccgt gttcgccaaa ttcgccaccc
     1801 aatccgtctc gtccgccacc acagactcaa tctgcggcaa cacctcccga taaagcgcgg
     1861 ccttgtccga agccgaaaaa tgaagcgcgt gcatcacatc tcctatagtt gcatacatat
     1921 caggcggcca ttataaaaca acctgcccga aacaacattc caaaccgccc ccgcagccgc
     1981 ccgccccaag ttgcgaaccc gccgcatatc cactaaactt cacattgcaa cgcgccacac
     2041 gcggcagaca gaaaaaacac gacacggagc aaaaaagatg tatcaccaaa tcggaatgtg
     2101 ggatcaaaaa tgggtcatcg gcaactggaa aatgaacggc cggctccaga acaacaattc
     2161 gctcatgcac cgcttccgca tcctccccac cgccgaacgc gtcctcatcg gactcgccgc
     2221 cccgaccgtt tacctgctgc aactgcacaa cgccatgcaa atcgtcttaa acaaccgcat
     2281 cctcacctgc gcccaagacg tgagccgctt ccccgacaac ggcgcgtaca ccggcgaagt
     2341 gtccgccgaa atgctcgccg acaccggcac agacatcgtc ctcatcggac actccgaacg
     2401 cagcctttat ttcggcgaaa aaaacgaaat ccaacgccgc aaaatggaaa acgtcctcaa
     2461 cgtcggactc atcccgttat tgtgcgtcgg cgaaagcctc gaagagcgcg aagccggcaa
     2521 agaacacgaa gtcatcgccc atcagctttc catcctgcaa ggactggaca ccaaaaacat
     2581 cgccgtcgcc tacgaacccg tctgggcgat cggcaccggc aaagtcgcca ccgtcgaaca
     2641 gattgccgat atgcacgcat tcatctacaa agaaatcttg tctttgtgcg gaagcgatgt
     2701 taaaatccgt gtcctttacg gcggaagtgt gaaagcggac aacgcggccg acatcttcgc
     2761 agtaccttat gtggacggcg cactcgtcgg cggcgcgtca ttgtcgtacg actcctttac
     2821 cgccatcatc agtgccgcac aaaatgcgta gaaaaatatg gaagccttca aaaccctaat
     2881 ttggattgtt aatataattt ccgctttggc cgtcatcgtg ttagtattgc tccaacacgg
     2941 caaaggcgcg gatgccggcg cgaccttcgg atcgggaagc ggcagcgcgc aaggcgtatt
     3001 cggctctgcc ggcaacgcta acttcctcag ccgttcgacc gccgttgcag caacattttt
     3061 ctttgcaacc tgcatggcta tggtgtatat tcacacccac acgacaaaat acggtttgga
     3121 cttcagcaac gtacaacaaa ctcagcaagc acccaaaccc gtaagcaata ccgaaccttc
     3181 tgcccctgtt cctcagcagc agaaataaca gtttttcaaa tgccgacatg gtgaaattgg
     3241 tagacacgct atcttgaggg ggtagtggcc gtaggctgtg cgagttcaaa tctcgctgtc
     3301 ggcaccaaca cacaaaacgc ttagaaattc taagcgtttt tttcgtttaa tcctgtctaa
     3361 tgccgtcaac caatcccaat caatacaagg atttcaggct gattgttatc ctgccgtccc
     3421 ccttcctgac agtgcaatcc cgtccaatcc gccctaattg aagtaaccta aaatttacgg
     3481 tatcttttgc ggtatctgaa aaatacctcg aaaaaatacc gcaaaaataa agctgaacga
     3541 ccgccaaatc aggaatgcca agcggaaaag agcttgcggg gaatactgcc aagacgtagg
     3601 gaacaagggg gaaaccgtcc aagatgcagg gcggtttttt ttgggttttt ggaaaaaacc
     3661 tatactagga agcgataccc ttagttgtta ccttgttacc ggggaaaagt tagataaata
     3721 agcatatgaa
//
LOCUS       DAWFAN010000170         3729 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWFAN010000170 DAWFAN010000000
VERSION     DAWFAN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.170"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            58..345
                     /locus_tag="WGO65_001800"
     CDS             58..345
                     /locus_tag="WGO65_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328055.1"
                     /translation="MDDLIAAFLGYLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFSCSVCLLFYFLLFIAETIRKTINKIK"
     gene            616..840
                     /locus_tag="WGO65_001801"
     CDS             616..840
                     /locus_tag="WGO65_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328056.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            928..1401
                     /locus_tag="WGO65_001802"
     CDS             928..1401
                     /locus_tag="WGO65_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328057.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEKYVFHTQADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
     gene            1963..2234
                     /locus_tag="WGO65_001803"
                     /pseudo
     CDS             1963..2234
                     /locus_tag="WGO65_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2397..2945
                     /locus_tag="WGO65_001804"
     CDS             2397..2945
                     /locus_tag="WGO65_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328058.1"
                     /translation="MKKQITAAMMILTMIAAPAMANSMDNQIFENQVFHTRADAPMQL
                     AELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIP
                     GGVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPG
                     QGIGRHRPWESKSTDRSWKNRF"
     gene            2927..3292
                     /locus_tag="WGO65_001805"
     CDS             2927..3292
                     /locus_tag="WGO65_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328059.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            3350..>3729
                     /locus_tag="WGO65_001806"
     CDS             3350..>3729
                     /locus_tag="WGO65_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328060.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
BASE COUNT         1174 a          561 c          802 g         1192 t
ORIGIN      
        1 aatggcaaaa caggtaatga attaaagaca ttacctgttt ttacagaaat ttttaaaatg
       61 gatgatttga ttgcagcttt tttaggctat ttatcaggta aatatagcga agggaaaata
      121 agcagattag aaatatctct aacggcagga agcattttct ttgcagtatt ctttattcat
      181 gaactgataa tttttattgt tgatggaaat aaggaaaatc tttggagttt tattactggg
      241 ataatgaaag atttattttt attttcatgt tctgtttgtt tattattcta tttcttatta
      301 tttattgcag aaactattag gaaaacaatc aataaaatta aataagatat ttaagccgct
      361 aaaaaccatt taagtattta atcttttcta tgcggcattt atcttggaat tgaaatactg
      421 caatcgggag gctaaggctt ccgttgtaac gtttaccata tatttcatta ttttaatttc
      481 atcaaaaaaa gaaattcaag attatacata ccgccaatag tttcttgatt gttttgtata
      541 tttatctgaa atatcaacat ctctctatct tattccgaat tttattgttg aaacttgcat
      601 gaaaaaccat acaagatgat gaaaaacaaa tatattttaa taaaagcagc aatcggcata
      661 accgccatat ctatatttta tcttctaact caagaaagca gcggcaaaac tgaagaacca
      721 tcatatttct tgatgtttat gtttctcaac ttgctttggt ttgaagaaaa taaaacagtt
      781 atggctgccg ttacggcaat gattgccgcc cactttatat ttgtcgcact atccgattaa
      841 taagcaatca aaaaggctgc ttggaaattt caggcagcct ttcaggagga ttttgatgga
      901 acattatctc ctccctaaga atttgttatg aaaaaacaaa tcactgaagc cacgatgatg
      961 ctgtctataa tcgcccccgc aatggcaaac ggattggaca atcaggcatt tgaaaaatac
     1021 gtattccaca cgcaggcaga tgcgccgatg cagttggcgg agctttctca aaaggagatg
     1081 aaggagactg agggggcgtt tcttcctatt gtggcaggtg ctttgatggg aggggcaatt
     1141 agtgcttggg ctaatcacgg catttctaaa attaaaacag ggcaatttgc ttcaacaaga
     1201 agtaccttgg ctgcaaccgc tggtggtatg ataggaggcg gatatagcag tgcgatgttg
     1261 cgcggtgcag ggattactac tagtgttttt gcccgttctg cttggaaagg gggaaatgct
     1321 attcctaatg cagttattcg tgcaaatggt gcagcattaa atcagtcttc tagcgccgca
     1381 atgggaaaac atagaagata aacgaaatgg atgatctaat actatatttt ttatcaggta
     1441 tattcggaaa tcaagttgct gaatatatta taaaaaacaa ccgtgagata aaagttccct
     1501 ttattgtgct ttacgcaata ttttttacgt tgatttatac tgtggctttg ttgtttctca
     1561 gtctgatcta ttgggtcaat ggtgcggaga ttgcatggaa ggggataggt attttcagta
     1621 tgtcggtcag tttttgtata gtcttctgtc tttatttgat tgacaaggca ggaagatgta
     1681 aggataagaa acaataggga tcagatgcgg cagcaaacgt agattgggtc taggctcaac
     1741 ctgcgtttgc tgcatctttg gaagaaatag tggattcgaa aaaataggaa gaattctgat
     1801 taatggtatg cgaggtgtag cagtggggac tgttgctggt ggcataactg gttatgcagg
     1861 ttctacgggt aagaacactg acattcgccg ttaaataata aataaaccaa cttccaacca
     1921 aacctgctat cggtttggtt ggaagtgaga ggaggtaagg tagtgtttgc tgttattttc
     1981 ttttcgacat tgggctgtat tttagcatgg ataagagata ttcctaaaat aaaatcaaaa
     2041 aaaattttgg caagatcctt atatattata ggaataataa atgtaataat cggctatgta
     2101 ttaattaaaa atatattggt ttctgtttcg gatggaggtg gaataaaata tgttgcaata
     2161 tatttatcaa atcttttttt tggacagtat tgatgtatgt tcttgtgaaa agattatcta
     2221 aaaagccaag ctgaaaccca gtacacaact catcaataaa aatccctttt gcacggaaca
     2281 atgacaatat tcaaattgct ggatttgcaa tctgatttac ctattgaaat tgggtgtgca
     2341 acatacgttc taagcagggc tttgattttc caaccttgta atccaaagga gttattatga
     2401 aaaaacaaat taccgcagcg atgatgattc tgacaatgat tgccgctccg gcgatggcaa
     2461 acagcatgga caatcagata tttgaaaacc aagtattcca tacgcgggca gatgcgccga
     2521 tgcagttggc ggagctttct cagaaggaga tgaaggagac tgagggggcg tttcttccat
     2581 tggctatctt gggtggtgct gccattggta tgtggacaca gcatggtttt agttatgcaa
     2641 cgacaggcag accagcttct gttagagatg ttgctattgc tggcggatta ggcgcaattc
     2701 ctggtggtgt aggtgccgca ggaaaggttg tttcctttgc taaatatgga cgtgagatta
     2761 aaatcggcaa taatatgcgg atagcccctt tcggtaatag aacaggtcat cctattggaa
     2821 aatttcccca ttatcatcgt cgagttacgg ataatacggg caagactttg cctggacagg
     2881 gaattggtcg tcatcgccct tgggaatcaa aatctacgga cagatcatgg aaaaaccgct
     2941 tctaactcct cctttttttc tatttgaaga tgtgtctttg gatatatttc aaggattgtc
     3001 ggaattggaa aagaaaatag agccgcagga tttgatggat gatgtttacc gtgcttttga
     3061 ttcggttgga aatattctga atttccgtat agtagaaaaa gagcaaaaag ggttttgggt
     3121 aagtaccaaa atcaaaacag ttgtatttga ttcggcagat atgtcttctg atgatctttt
     3181 tctgaaatgc ttacagtctt catataaggc ttattttgaa actgaacctg ccggtttaga
     3241 taaacggcag ctgatgaaaa cgttaattca aaaatgcgga ttcagttgtt gacgaaaaaa
     3301 tggattctga actaacagaa actgatttcg accatgtatg aggtaaaaaa tgtctttcgg
     3361 ctatttgatt gcaacatcgc aaccttgcga attgttgaca aaatctcgag gtgaaacttt
     3421 ttctttaatt atggataaga tggatttatg gatttatttc agattttgtg aaggaaatat
     3481 ttatacaata aggaagaatg aaactgaatc ttgtcttaca gaaagaggag gcaaatggtt
     3541 gaaacatatt tatgaattta atcggggaag ttttattttt tcttatgtgc ttctaaaaaa
     3601 gagagaaagc gaagagaatt ttgcagaaat tgttttaaaa tcaataaaaa ataataaaat
     3661 tttgacagtt aaggtacggt caggcttgca ttttgactta agaaatattt atcgtattga
     3721 gatgtatag
//
LOCUS       DAWFAN010000171         3725 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWFAN010000171 DAWFAN010000000
VERSION     DAWFAN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.171"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(88..906)
                     /locus_tag="WGO65_001807"
     CDS             complement(88..906)
                     /locus_tag="WGO65_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0328061.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTGLSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(973..2223)
                     /locus_tag="WGO65_001808"
     CDS             complement(973..2223)
                     /locus_tag="WGO65_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0328062.1"
                     /translation="MIKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVAADDPFIRNIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="WGO65_001809"
     CDS             complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="WGO65_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ0328063.1"
                     /translation="MSDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGSESLPL"
     gene            2871..3683
                     /locus_tag="WGO65_001810"
     CDS             2871..3683
                     /locus_tag="WGO65_001810"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ0328064.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          826 a          906 c         1116 g          877 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcgggaaa tatccatatc
      121 aggctgtggg ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggag aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatgcagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaaag gattttctat tttgggagct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttagataa aggggtgtag tctgtcccgc ttatggtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttcgg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggctcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactgt acacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gtccgttaaa acagataaag gaagccgatc ggacaatccg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggtgga agtaggttta tgcctgcctt catccgacta
      961 ccgccttttc aatcaacccc gcttcaatac ggatgtattg atgtagcgtt ggacacccga
     1021 ggcaatggat tgggcgcact gccggcggaa ggattcgctg cccagcaact tctcttcagc
     1081 agggttggac aggaaggcgg tttcgaccag gatagacggc atatcgggtg cgcgcaaaac
     1141 ggcgaaattg gcttcgtcca ccctgccttt gtgcaagtgg ttgagcctgc ccaattcttc
     1201 aagcaccagt ttgccgagtt tgcggctgtc gcgcagcgtg gcggtttggg tcatatcgag
     1261 cagggcggta tcgacgttgc ggttgccgct ggtcggtacg ccgccgaccg cgtcggcatt
     1321 gttttgcgtc tgttccaaga atttggcggc ggagctggtc gcgcctttgg tgttcagcat
     1381 atagaccccc gttccgcgcg cggaggggct ggtgaaagcg tcggcgtgga tggagacgaa
     1441 tacgtccgcc cgccgtgcgc gccctttggc gacacgcacg cccaaaggga tgaacacgtc
     1501 ttcgttgcgc gtcataaaga cgttgtaacc caaggcttcc aattgttttt tggtttcgcg
     1561 ggcgatggag aggacgacgt gtttttcctg caaaccgccc gggctgacgg cgccggggtc
     1621 ttcgccgccg tgtccggggt cgatcataat gacgggtctg cgcccgtttt tgccgcgttc
     1681 ggtttgtgca ggagtgtttt gagcgaggtc ggcttcaggg gagccgcgac gggttttgtt
     1741 caggctgccg ttaagcagag ccatcatcgg atcgtcggga tccatccctt gcggatagag
     1801 atcgacgacg aggcggtttt taaagccgcc gacggggggg agggaaaaaa cttgcgcgta
     1861 ggaaggctgt ttcaaatcga tgacgaggcg gacggtgtcg ggcgtgttct gaccggcgcg
     1921 tatattgcgg ataaaggggt cgtccgccgc cactttttgg gacagtccgt gcaatacggt
     1981 attgatgttt gcgttttgta tgtcgacgac cagcctgccc ggattgtcga gcgcgaagtg
     2041 ctggtatttg agcgcggcgg tgctttccag cgttaggcgg gtgtaggtgt gcgacggcca
     2101 tatccgtgcg gcggtgaatt gcggggcgcg taccgttttg gcaacggcgg atgcgatggg
     2161 gcttagggcg aacagtgtgc cggcggtgcg gcggatgatt tgtcttcgtg tcagtttgat
     2221 catagcggca ggctttcgct tcctcgttcg gtatgggcgg tcagcaggca ttttctgccg
     2281 ccgccgccgt gtgtcaatgt tgcggtgatg tcggcgggcg gcgtaaattc cccgccctgt
     2341 tgcggccatt cgatcaggca gacgctgttt gcggcaaaca gttcgtcaag ccccgcgtct
     2401 tcccattctt cggggaacga gaagcggtag aggtcgaaat ggtgcaaggt gaagcgttcc
     2461 agcggataag attcgacgat ggcgtaggtc gggcttttga ctgcgccctg atgacccaat
     2521 ccgcgcagga ttccgcgtgt cagcgtggtt tttcccgcac ccaaatcccc ttcgaggtaa
     2581 atgaccagcg gtgcgtttaa acgggaagac cacgccgcac ccaaatcgag tgtggcggct
     2641 tcgtcggcaa ggaatcggga aacgggagga aaatcagaca taggggcttt acaactttta
     2701 tgtggcggat cggaggggca ggcggtcgga aaaccgtgcg cccggcggta gcattgccgt
     2761 atattataac agccaaatcc gcatcctgct ttcagacggc aacggctgtc aagaaaaagc
     2821 ggcacgtgta caatacgcag attgtatgtt taggacggat tggaaaaagg atggaaaata
     2881 tcggcaggca gcgacccatc ggcgtttttg actcgggaat cggcggtttg accaatgtgc
     2941 gcgcgctgat ggagcggctg ccgatggaga acatcattta tttcggcgat acggcgcgcg
     3001 tgccttacgg gacgaaatct aaggcgacca tcgaaaattt ctcgatgcag attgttgatt
     3061 ttttattgga acacgatgtc aaggcgatgg ttatcgcgtg caacacgatt gcggcggtgg
     3121 cggggcagaa aatccgtcaa aagacgggca atatgccggt tttggacgtg atttccgccg
     3181 gcgcgaaagc cgcgctggca acgacgcgca acaacaaaat cggcattatc gccaccaata
     3241 cgacagtcaa cagcaacgcc tatgcgcgcg ccatccatag gaacaacccc gacacgcttg
     3301 tccgcacgca ggccgcgccg ctgctcgtcc ctttggtgga agagggctgg ttggaacacg
     3361 aagttacccg cctgaccgta tgcgaatacc tcaaaccatt gcttgcagac ggcatcgata
     3421 cgctggtgtt gggctgcacg cactttccct tgctcaagcc cttaatcggc agggaggcgg
     3481 gcaatgtcgc gttggttgat tctgcaatta caacggccga agaaaccgca cgcgtccttg
     3541 ctcaggaagg attgctcaat accgacaaca acaatcccga ctaccgtttt tacgtcagcg
     3601 atattccttt gaaattcaga accatcggcg agcgttttct gggcaggacg atggagcaga
     3661 ttgaaatggt gtctttgggt taaaacgatg acggaaagct gcccgagatt acagaaacct
     3721 aaaat
//
LOCUS       DAWFAN010000172         3717 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWFAN010000172 DAWFAN010000000
VERSION     DAWFAN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.172"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..329
                     /locus_tag="WGO65_001811"
     CDS             <1..329
                     /locus_tag="WGO65_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ0328065.1"
                     /translation="IATTVPAQQTFLDAAELMQWSIETEGLGLNVISHKILGKDHAQV
                     EFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKACCGK
                     YLKPVA"
     gene            complement(547..1341)
                     /locus_tag="WGO65_001812"
     CDS             complement(547..1341)
                     /locus_tag="WGO65_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ0328066.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQM
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTVRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1445..2170
                     /locus_tag="WGO65_001813"
     CDS             1445..2170
                     /locus_tag="WGO65_001813"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ0328067.1"
                     /translation="MPRFHLPENLSVGQTVALPENIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLADIRHPSGAIVFMVGPEGGWTEQEEQQAFDAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFA"
     gene            complement(2789..3631)
                     /locus_tag="WGO65_001814"
     CDS             complement(2789..3631)
                     /locus_tag="WGO65_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0328068.1"
                     /translation="MRNYVISLASAKERRAHIADTFGRHDIPFQFFDALMPSEELNRV
                     MAELVPGLAKQHLLSEVEKACFMSHVVLWKQALDEGVPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPTEWIKAVDWMMFTYFFDKEGMPVYQVSPALCTQELHYAKFLSQN
                     SMLGSDLEKDREQGRRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1018 a         1106 c          776 g          817 t
ORIGIN      
        1 tcatcgctac taccgttccg gcgcaacaaa cattcctcga tgccgccgaa ctgatgcaat
       61 ggagtataga aaccgaaggg ctgggcttga acgtcatctc gcacaagata ctcggcaaag
      121 accacgccca agtcgaattt gaagcctact tccgagacgg acaacaccga tccgcgcatc
      181 acgaactgtc cggcttcgtc aacatcggcg gacaatggta ttttatcgat cccaccgttc
      241 cgcatcctgc gatgaaacaa ccctgcattt gcggatcggg caaaaaattc aaagcctgct
      301 gcggcaaata tctgaaacct gtcgcataaa aatgccgtct gaacgttcag acggcatttt
      361 caacacgcaa aaaaaaccat tcataccaag ggtaagtatg aatggtcaat acattgcggg
      421 aaaacgtctt acttgctgca ctgccgaaaa gggagaaacg gcagcggtaa tcagcgaaaa
      481 ggattgtacc cgaattaatg ttaagaaacg ttaatcgtga aaatatatta acaaacctgt
      541 tgaaacctat tggttttccc gtatccaccc gacccagcgt tcaaacagct tcggttcgag
      601 cgcggcaacg accgagcgtt tgaacacgtg ttcaccactc caaaatccgt cgccttccaa
      661 agtcgtcagc ctgccgcccg cctcctcaaa aatcagcgca ccggcggcat aatcccatag
      721 cttctgcccg ccgtggacat aaacatcata acgcccgcac gccagataac accagtccag
      781 cgtgctgctg cccatactcc gcaccgtacc gaaaggcgcg agcgtgctca tccggctgga
      841 cagcttgccg gaacgcagat atttgatttc cacacccgca attgcctcat tcaacttctt
      901 atccacgagg cgcaaaggca ggcgtgtccc attaagaaac gcaccctgcc cccgttcggc
      961 ataaaaacat tcgccgctga ccggattgta gattacgccc aattcggcgc gcccgttgcg
     1021 gacaaacgcc accgataccg caaaatgcgg cagcccgttg acaaaattgt tcgtcccgtc
     1081 tatcggatcg acaatccaca gccccttttc ccccgaatat tgttcccaca aagccgactg
     1141 ttcctgccgc gacatttcct cgcccaacat cgggcaatcg atcagaagcg gcaacgcggc
     1201 ggcaaaagcc atctgcgcgg caatgtccgc ctcgctcaac atcgaaccat cttccttgcg
     1261 gcaagacggc gtattcaaaa aacgcggcat aatttcggtt tgcgcgatat ggcgcacgac
     1321 tttctgcaaa cggtgcaaca cttcctactg tcctcatatt ttgaacttgc ggcgcgcgaa
     1381 cgtataatgt ccgtttccat cacgccgcta cgacggatta taaccgtccg aaccgccaaa
     1441 aactatgccc cgattccacc tgcccgaaaa cctttccgtc ggacaaaccg tcgccctgcc
     1501 cgaaaacatc gtccgccacc tcaacgtcct gcgcgtccgc cccaacgaaa acatcaccct
     1561 cttcgacggc aaaggcaagg cacacgccgc acggctgacc gttttggaaa aacgccgcgc
     1621 cgaagccgaa atcctgcacg aagacacaac cgacaacgag tccccgctca acatcacact
     1681 gatacaatcc atctcctccg gcgatcgcat ggatttcacc ctgcaaaaaa gcgtcgaact
     1741 cggcgtaacc gccatacagc ccgtcatcag cgaacgctgc atcgtccgcc tcgacggaga
     1801 acgcgccgcc aaacgcctcg cacgctggca ggaaatcgtc atctccgcgt gcgaacagag
     1861 cggcaggaac accgttcccc ccgtactgcc catcatcggc taccgtgaag cactcgacaa
     1921 aatgccgtct gaaagcacca agctgattat gagcatcaac cgcgcccgca aacttgccga
     1981 catccgccac ccgtccggcg caatcgtctt tatggtcgga cccgaaggcg gctggacaga
     2041 acaggaagaa caacaggcat ttgatgccgg ctttcaggcg gttacactcg gcaaacggat
     2101 tttacgcaca gaaaccgccc cactcgccgc catcgccgca atgcagacgc tttggggcga
     2161 tttcgcataa acagaaatgc cgtctgaaac ccgttcagac ggcattttgc agccgattaa
     2221 gatagtaggt tcaaataaga tttcccgtgc cgtcattccc gcgaaagcgg gaatctagaa
     2281 acggaaaaac gcagaaattt atccggaaaa acaacaatcc ttccgccgtc cttcccgcgc
     2341 aggcgggaat ctagaatttt aatacgacaa gaatttatcg gaaaaaccga agtttaaaga
     2401 cctagattcc cgcctgcgcg ggaatgacaa attttaggtt gctgtttttt ggttttctgt
     2461 ttttgcggga atgacgaatt ttagatttct gttttttggt tttctattct tatgaaaata
     2521 acgagattct agcttgtggg tatttatcgg gaaaacagaa acccctccgc cgtcattccc
     2581 gcgcaggcgg gaatctagaa ttttaatgcg gcaagaattt atcggaaaaa accgaagttt
     2641 aaagacctag attcccactt tcgcgggaat gacgaagtgg aagttgcccg aaacttaaaa
     2701 caagcgaaac cgaacggact agattcccgc ctgcgcggga atgacagtgt gtccatttct
     2761 aattctaatc cgctatattt tacacaaact atttgaacga tatgacccgc ctgccgtaag
     2821 ctttctcaag ctccgcctgc ctttgacgct ccattctttt cttcttttcc ctaccgaatt
     2881 tacccaaagc acgcttcaag tcaaacatca ccttcaacga acggcggtgt cttcttcctt
     2941 gttccctatc tttttccaaa tcgctgccca acatactgtt ttgactgagg aacttggcat
     3001 aatgcaattc ttgggtacat aaggcgggac taacctgata aacaggcatc ccctctttat
     3061 caaagaaata agtaaacatc atccaatcta ccgctttaat ccactctgtc ggcaaaacgg
     3121 caaacctttc caagaaaaac cgcatcgcct cacgcgaaat gatatagcca gccgtccccc
     3181 aatgttcgct ctccagcaaa ggaaatgacc ggttttcata attcaggact ttatctggtc
     3241 tgacaataac tttcgcaaac atcgtttcca aacggacaat aaaggcagaa tccttatcaa
     3301 aacgctcttc caaccaagta tcttcggcaa ggaacttttc tgcgtctttg ccaagcagga
     3361 catcatcctc aaatacggca acatacggta cgccttcgtc caacgcctgt ttccacaata
     3421 cgacgtggct cataaagcag gctttttcca cttcactcaa caggtgctgt tttgccaatc
     3481 ccggcaccaa ttccgccatc acccgattca gttcttcaga cggcatcagt gcgtcgaaaa
     3541 actgaaacgg aatgtcgtgc ctgccgaaag tatcggcaat gtgcgccctg cgctctttgg
     3601 cagaagcaag gctgataaca taatttctca tcagaacctc ccttaaaact ggaaccgtcc
     3661 cgttcacaat aaacacatca aaaaaatttt tgaatcagaa acacatatgg ctttggc
//
LOCUS       DAWFAN010000173         3705 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWFAN010000173 DAWFAN010000000
VERSION     DAWFAN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.173"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            262..603
                     /locus_tag="WGO65_001815"
     CDS             262..603
                     /locus_tag="WGO65_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0328069.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGTSAPDFNAGGTG
                     IGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPRICIPE
                     TLQTQMTHTRI"
     gene            600..3461
                     /locus_tag="WGO65_001816"
     CDS             600..3461
                     /locus_tag="WGO65_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEZ0328070.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLS
                     APYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIG
                     AVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKG
                     GSQLQLHGNNTYTGKTVIEGGSLVLYGNNESDMRVETKGALIYNGAASGGSLNSDGIV
                     YLADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAIIGGKLYMSARGKGAG
                     YLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRG
                     NAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMP
                     GIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLD
                     HNATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTW
                     SENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGT
                     LMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSITEGTLVGL
                     AGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVA
                     GLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1003 a         1012 c          986 g          704 t
ORIGIN      
        1 agggaaattc aaattaagtt agaattatcc ctataagaaa aagccgtcta agccggtata
       61 aacaaaataa actcattgag ctatttgtcg aaagttcaaa tttccatttt aaaacaatta
      121 gtaaaatcga gtttatccta gttgtccaag acaaccccta taataatata attcaaaata
      181 taaaaatggg ttacatctaa acattacgga atttttattc cctcgcctga attctattgt
      241 cagattcaag gagacctcat catgcgaacg accccaacct tccctacaaa aactttcaaa
      301 ccggctgcca tggcgttagc tgttgcaaca acactttctg cctgcttagg cggcggcact
      361 tctgcgcccg acttcaatgc aggcggcacc ggtatcggca gcaacagcag ggcaacaaca
      421 gcgaaatcag cagcagtatc ttacgccggt atcaagaacg aaatgtgcaa agacagaagc
      481 atgctctgtg ccggtcggga tgacgttgcg gttacagaca gggatgccaa aatcaatgcc
      541 cccccccccc gaatctgcat accggagact ttacaaaccc aaatgacgca tacaagaatt
      601 tgatcaacct caaacctgca attgaagcag gctatacagg acgcggggta gaggtaggta
      661 tcgtcgatac aggcgaatcc gtcggcagca tatcctttcc cgaactgtat ggcagaaaag
      721 aacacggcta taacgaaaat tacaaaaact atacggcgta tatgcggaag gaagcgcctg
      781 aagacggagg cggtaaagac attgaagctt ctttcgacga tgaggccgtt atagagactg
      841 aagcaaagcc gacggatatc cgccacgtaa aagaaatcgg acacatcgat gtggtctccc
      901 atattattgg cgggcgttcc gtggacggca gacctgcagg cggtattgcg cccgatgcga
      961 cgctacacat aatgaatacg catgatggaa ccaagaacga aataatgtct gcagccatcc
     1021 gcaatgcatg ggtcaagctg ggcgaacgtg gcgtgcgcat cgtcaataac agttttggaa
     1081 caacatcgag ggcaggcact gccgaccatt tccaaatagc caattcggag gagcagtacc
     1141 gccaagcgtt gctcgcctat tccggcggtg ataaaacaga cgagggtatc cgcctgatgc
     1201 aacagagcga ttacggcaac ttgtcctacc acatccgtaa taaaaacatg cttttcattt
     1261 tttcggcaag caatgacgca caagctcagc ccaacacact gaccctattg ccattttatg
     1321 aaaaagacgc tcaaaaaggc attatcacag tcgcaggcgt agaccgcagt ggagaaaagt
     1381 tcaatggctc caaccattgc ggaattactg ccatgtggtg cctatcggca ccctatgaag
     1441 caagcgtccg tttcacccgt acaaacccga ttcaaattgc cggaacatcc ttttccgcac
     1501 ccatcgtaac cggcacggcg gctctgctgc tgcagaaata cccgtggatg agcaacgaca
     1561 acctgcgtac cacgttgctg acgacggctc aggacatcgg tgcagtcggc gtggacagca
     1621 agttcggctg gggactgctg gatgcgggta aggccatgaa cggacccgcg tcctttccgt
     1681 tcggcgactt taccgccgat acgaaaggta catccgatat tgcctactcc ttccgtaacg
     1741 acatttcagg cacgggcggc ctgatcaaaa aaggcggcag ccaactgcaa ctgcacggca
     1801 acaacaccta tacgggcaaa accgttatcg aaggcggttc gctggtgttg tacggcaaca
     1861 acgaatcgga tatgcgcgtc gaaaccaaag gtgcgctgat ttataacggg gcggcatccg
     1921 gcggcagcct gaacagcgac ggcattgtct atctggcaga taccgaccga tccggcgcaa
     1981 acgaaaccgt gcacatcaaa ggcgatctgc agctgggcgg cgaaggtacg ctgtacacac
     2041 gtttgggcaa actgctgaaa gtggacggta cggcgattat cggcggcaag ctgtacatgt
     2101 cggcacgcgg caaaggggca ggctatctca accgtaccgg acaacgtgtt cccttcctga
     2161 gtgccgccaa aatcgggcgg gattattctt tcttcacaaa catcgaaacc gacggcggtc
     2221 tgctggcttc cctcgacagc gtcgaaaaaa cagcgggcag tgaaggcgac acgctgtcct
     2281 attatgtccg tcgcggcaat gcggcacgga ctgcttcggc agcggcacat tccgcgcccg
     2341 ccggtctgaa acacgccgta gaacagggcg gcagcaatct ggaaaacctg atggtcgaac
     2401 tggatgcctc cgaatcatcc gcaacacccg agacggttga aactgcggcc gccgaccgca
     2461 cagatatgcc gggcatccgc ccctacggcg caactttccg cgcagcggca gccgtacagc
     2521 atgcgaatgc cgccgacggt gtacgcatct tcaacagtct cgccgctacc gtctatgccg
     2581 acagtaccgc cgcccatgcc gatatgcagg gacgccggct gaaagccgta tcggacgggt
     2641 tggaccacaa cgctacgggt ctgcgcgtca tcgcgcaaac ccaacaggac ggtggaacgt
     2701 gggaacaggg cggtgttgaa ggcaaaatgc gcggcagtac ccaaaccgtc ggcattgccg
     2761 cgaaaaccgg cgaaaatacg acagcagccg ccacactggg catgggacac agcacatgga
     2821 gcgaaaacag tgcaaatgca aaaaccgaca gcattagtct gtttgcaggc atacggcacg
     2881 atgcgggcga tatcggctat ctcaaaggcc tgttctccta cggacgctac aaaaacagca
     2941 tcagccgcag caccggtgcg gacgaacatg cggaaggcag cgtcaacggc acgctgatgc
     3001 agctgggcgc actgggcggt gtcaacgttc cgtttgccgc aacgggagat ttgacggtcg
     3061 aaggcggtct gcgctacgac ctgctcaaac aggatgcatt cgccgaaaaa ggcagtgctt
     3121 tgggctggag cggcaacagc atcactgaag gcacactggt cggactcgcg ggtctgaagc
     3181 tgtcgcaacc cttgagcgat aaagccgtcc tgtttgcaac ggcgggcgtg gaacgcgacc
     3241 tgaacggacg cgactacacg gtaacgggcg gctttaccgg cgcgactgca gcaaccggca
     3301 agacgggggc acgcaatatg ccgcacaccc gcctggttgc cggtctgggc gcggatgtcg
     3361 aattcggcaa cggctggaac ggcttggcac gttacagcta cgccggttcc aaacagtacg
     3421 gcaaccacag cggacgagtc ggcgtaggct accggttctg acggacagga agcagacagc
     3481 cgcaaagatc acggtctttg cggctgtttc ttatgaaaag aaaaccctat tccaattgcc
     3541 tgcttctatt gtttcaagac ttcttccaaa gattcggcat caatcagatg tatagcggat
     3601 taacaaaaat caggacaagg cggcgaagcc gcagacagta cagatagtac gaaaccgatt
     3661 cactcggtgc ttcagcacct tagagaatcg ttctctttga gctaa
//
LOCUS       DAWFAN010000174         3603 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWFAN010000174 DAWFAN010000000
VERSION     DAWFAN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.174"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            41..982
                     /gene="mafA"
                     /locus_tag="WGO65_001817"
     CDS             41..982
                     /gene="mafA"
                     /locus_tag="WGO65_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0328071.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATRYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            986..2482
                     /gene="mafB"
                     /locus_tag="WGO65_001818"
     CDS             986..2482
                     /gene="mafB"
                     /locus_tag="WGO65_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0328072.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADGYDGPKGGNYPKPTGA
                     RDEYTYNVNGVARSIKLNPTDTRDVRQRISDNYSNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKF
                     AVIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKA
                     AVSGDFSAAYNTRITRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPNHINVLSGNSI
                     QHILYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEISDIVTSPKTQWYAQTGTGGKY
                     IAKGRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK"
     gene            2497..2727
                     /locus_tag="WGO65_001819"
     CDS             2497..2727
                     /locus_tag="WGO65_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0328073.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            2876..3552
                     /locus_tag="WGO65_001820"
                     /pseudo
     CDS             2876..3552
                     /locus_tag="WGO65_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
BASE COUNT         1118 a         1027 c          780 g          678 t
ORIGIN      
        1 atttacactc aaactcgttc aaaacaaaac aaaccctgcc atgaaaaccc tgctcctcct
       61 catccccctc gtcctaaccg cctgcggcac actgaccggc atacccgccc acggcggcgg
      121 caaacgcttt gccgtcgaac aagaactcgt cgccgcatcg tcccgcgccg ccgtcaaaga
      181 aatggatttg tccgccctga aaggacgcaa agccgccctt tacgtctccg ttatgggcga
      241 ccaaggttcg ggcaacataa gcggcggacg ctactctatc gacgcactga tacgcggtgg
      301 ctaccacaac aaccccgaaa gtgccacccg atacagctac cccgcctacg acactaccgc
      361 caccaccaaa tccgacgcgc tctccagcgt aaccacttcc acatcgcttt tgaacgcccc
      421 cgccgccgcc ctgactagaa acagcggacg caaaggcgaa cgctccgccg gactgtccgt
      481 caacggcacg ggcgactacc gcaacgaaac cctgctcgcc aacccccgcg acgtttcctt
      541 cctgaccaac ctcatccaaa ccgtcttcta cctgcgcggc atcgaagtcg taccgcccga
      601 atacgccgac accgacgtat tcgtaaccgt cgacgtattc ggcaccgtcc gcagccgcac
      661 cgaactgcac ctctacaacg ccgaaaccct taaagcccaa accaagctcg aatatttcgc
      721 cgttgaccgc gacagccgga aactgctgat tgcccctaaa accgccgcct acgaatccca
      781 ataccaagaa caatacgccc tctggatggg accttacagc gtcggcaaaa ccgtcaaagc
      841 ctcagaccgc ctgatggtcg atttctccga catcaccccc tacggtgaca caaccgccca
      901 aaaccgtccc gacttcaaac aaaacaacgg taaaaacccc gatgtcggca acgaagtcat
      961 ccgccgccgc aaaggaggat aaaccgtgaa accgctgcgc agactgacaa acctccttgc
     1021 cgcctgcgcc gtagcggccg ccgccttcgg acagcccgcc ctcgcggcgg acttggcgca
     1081 agatccgttc attaccgata acgcccaacg gcagcactac gaacccggag gcaaatacca
     1141 cctcttcggc gacccgcgcg gcagcgtttc cgaccgcacc ggtcaaatca acgtcatcca
     1201 agactatacc caccagatgg gcaacctgct catccaacag gcaaacatca acggcacaat
     1261 cggctaccac acccgctttt ccggacacgg acacgaagaa cacgccccct tcgacaacca
     1321 cgccgccgac agcgcaagca aagaacaagg caacgttgac gaaggcttta ccgtgtaccg
     1381 gctcaactgg gaaggacacg aacatcatcc cgccgacggc tatgacggcc cgaagggcgg
     1441 caattacccc aaacctacgg gagcacgcga cgaatacacc tataacgtta acggcgtagc
     1501 ccgcagcatc aaactcaatc cgaccgacac ccgcgacgtc agacaacgca tatccgacaa
     1561 ttacagcaac ctcggcagca atttctccga ccgcgccgat gaagccaaca gaaaaatgtt
     1621 cgagcacaat gccaagctcg accgctgggg caacagcatg gagtttatca acggcgtcgc
     1681 cgccggcgcg ctcaacccct ttatcagcgc gggcgaagcc ttgggcatag gcgacatact
     1741 gtacggaacg cgctatgcca tagacaaagc cgcaatgcgc aacatcgccc ccttgcccgc
     1801 cgagggcaaa ttcgccgtca tcggcggctt gggcagcgtg gcgggctttg aaaaaaatac
     1861 gcgcgaagcc gttgaccggt ggatacagga aaaccccaat gccgccgaaa ccgtcgaagc
     1921 cctggtcaac gtcctgccgt ttgccaaagt caaaaacctg acaaaggcgg caaaaccggg
     1981 gaaagctgcg gttagcgggg atttttctgc tgcatacaat acaagaataa ctagaaaagt
     2041 tactacagaa acagaggggt taaatagaat cagacagaac cagaaaaata gtaatataca
     2101 tgagaaaaat tatggaagag ataatcctaa tcatattaat gttttatctg gaaattctat
     2161 acaacatata ctgtatggag atgaagcagg aggtgggcat ctttttcctg gcaaacctgg
     2221 taagacaaca ttcccccaac attggtcagc cagtaaaata actcatgaaa ttagtgatat
     2281 cgttacatcc ccaaaaacgc aatggtatgc acagactgga acaggcggca aatatattgc
     2341 taaaggaaga ccagccaggt gggtatcata tgaaacgaga gatggaattc gtatcagaac
     2401 agtttatgaa cctgcaacag gaaaagtggt aactgcattc cccgatagaa cctctaatcc
     2461 caaatataac cctgtaaaat aatgagtatt tgaaagatga aaacattaga cgaacgaata
     2521 aaaaatttag gaaaatctct tgaagataga attgacgcta atctaattga tgccgcacta
     2581 gagtacatta ctttttcaga acgtttgcta gcttttgaaa cgctatgtga ttatatagaa
     2641 gatttcaata tccagcttac ggaaaaagaa tcccaagaaa tctcttttat taataaggaa
     2701 tttggaattg aaagtacatc agattaataa atattaggtc ggtttaaaaa cccaacaatc
     2761 aaaaagtcgt ctgaaactgt gtttaattta cagacgacct ttccttcatt tgaagcagga
     2821 tattgagaac tgagttcttc aaaaatccta cacctgctcc ttccacggca gcaccttggt
     2881 caaaacggca gacggctaca aagccattgc ccatattcaa gccggcgacc gcgtcttcgc
     2941 caaagatgaa accagtggaa aaactggata caaacctgtt accgcccaat acggcaatcc
     3001 gtatcgggaa accgtttaca ttgaaatttc agacggcatc ggcaacaacc aaaccctgat
     3061 ttccaataaa atccacccgt tttacagtca aggaaaatgg atacaggcag gtcgtctgaa
     3121 aaaaggcgac accctgcttt ccgaaagcgg tgcaaaacag acggttcaaa acattaccct
     3181 caaacagcag ccgctcaaag cctacaatct gaccgttgcc gactggcata cctacttcgt
     3241 caagggtaat caggcggaac ggaaggggtt tgggttcata atgcgtgtcc gccaagaaaa
     3301 acaccatcta cccccgtata tgggaatgat tcagaagcat atgcagcagc aaagaaattg
     3361 ggatatagaa aaataaaaga aagaacgaga aatgatgctg ctatttttaa aaaaggaaag
     3421 agttatatat cacgcgatgt agattcacat aatggagggg cttggaaaga agcatcttca
     3481 cccaagaagc tgaacagaaa agaaacaaga aacggtacat ttgataaaaa cctaaatcgt
     3541 attggagatt aattatgatg tgcgagttca aggattttag aagaaacatc ccttgtttta
     3601 agg
//
LOCUS       DAWFAN010000175         3371 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWFAN010000175 DAWFAN010000000
VERSION     DAWFAN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.175"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(198..578)
                     /locus_tag="WGO65_001821"
     CDS             complement(198..578)
                     /locus_tag="WGO65_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ0328074.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPSVQNQARPADF
                     REFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1054..3135
                     /locus_tag="WGO65_001822"
     CDS             1054..3135
                     /locus_tag="WGO65_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ0328075.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3125..3319
                     /locus_tag="WGO65_001823"
     CDS             3125..3319
                     /locus_tag="WGO65_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ0328076.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
BASE COUNT          739 a          997 c          861 g          774 t
ORIGIN      
        1 cattggataa cagccctgaa gaaatcggag caggtttaaa gttggcattg agccgctgcc
       61 gataggataa acccggaacg aaacgaaaaa taatccgcca cgcccgaaaa ccgccgaaac
      121 cagccgacag ccaaaacaaa tccgacacaa aaaaccgccc gaaaaatccg gacggcggtt
      181 caaacaggct gccccgttta gcgggcgcgg caggaagttt cgaccgaatt gccgtaggca
      241 tcggtaaagc cgaaaaaggc ttcgccgcct ttctggtgcc actcggttgc gtttccgaac
      301 aaaccgtgtt cggcggtata gcgttcgccg gatgcggcaa cgtcggaaga gaggacggca
      361 cgcctgccgt ccagccgcaa cgcgactttg ccgctgtcca aatggcggac gcgcacagac
      421 aaaccgttct cgcatgaaaa ctcccgaaaa tctgccgggc gggcttggtt ttgaacggac
      481 ggcatatgcc cgcgtccgcc gtcatcatac gcctccggca cggcacaggc cgccaaagac
      541 aaaaccggta cggtcagcgc gaaaaacctg atattcataa aagctcccca ataaaaataa
      601 gatatgaaac aaccgccctg attccaagct gcggcaacgc catactataa acggacgcgc
      661 aaacacacaa gcccgataac cggaatttac ccgcgatgaa tcaataatcc ggattgcgcg
      721 ccccttcttt acccgtcttc cggcgccgcc ttttgcctga cgatgccgtc caaacccgcc
      781 tgtccgcccg aaggaatgta aattttttcc aaattccaag taaaaaccgc tatcagtgtg
      841 ctaatttgcg ttaaaatcct attcggcgtt taacgttttg tgcgcccgca tccctgcact
      901 gtttgatgcg ggcataaggc acaaatcccg acaagcgcac tgtttcatac ttcgtcaatc
      961 attcagactc cggtttgtgc ccgtgccggc aggcggtcat ccgtttcccg ccgttcaggc
     1021 atattccgac agtgtgagat aaggatttat tcgatgaaat cactcaaaac cttcctcatt
     1081 tggggcatag tggtactggt cggcttagca tcctttacca ctctggctct cagccgaggc
     1141 gaacaggtca gcgcggtatg gatggttacc gccgccatat ccgtttactg catcgcctac
     1201 cgtttttaca gcctctacat cgccaaccgc gtaatgcggc tcgatcctaa ccgcctgact
     1261 ccggcagaac gccacaacga cggcttggac tacgttccga cgcacaaagg cgtattgttc
     1321 ggacaccact ttgccgcgat tgcgggcgcg ggccctttgg tcggcccggt tttggcggcg
     1381 caaatgggtt acctgcccgg tactttgtgg attatcttcg gcgtggtgtt tgccggcgcg
     1441 gtacaggata tgatggtctt gttcgtctct atgcgccgcg acggtaagtc tttgggcgat
     1501 attgtgaaac aggaactcgg cactgtcccc ggcgtgattg cctccatcgg tattttgatg
     1561 attatggtca tcattatggc ggtgttggcg ttgattgtcg taaaagcact ggttcacagc
     1621 ccttggggta cgtttaccat cgccgccact atgccgattg ccctgtttat gggtatttac
     1681 acgcgctata tccgtccggg caaaatcggc gagatttcca tcgtcggctt tattttgctg
     1741 atgctggcgg taatttacgg cgaagatgtg gcgaaaagtt ccatcggaca ttggttcgac
     1801 cttgacggca tccagctcac ttgggcgatt atgatttacg gctttgtcgc ctccgtattg
     1861 cccgtatggt tgctgctcac tccgcgcgac tatctctcca ccttcctgaa aatcggtacg
     1921 attgcggcct tggctttggg catcgtcatc gtcaatccca ctttgcaaat gcctgccgta
     1981 acccacttta tcgacggttc gggtccggta ttctcaggcg cattgttccc gttcctcttc
     2041 atcaccatcg cctgcggtgc ggtttcgggc ttccacgcgc tgatttcctc cggcaccacg
     2101 ccgaaaatgc tggaaaacga aacccacgtc cgtatgattg gttacggcgg tatgttgatg
     2161 gaaagtttcg tagccattat ggcgcttgcc gccgccgcat cgcttgatcc cggcgtgtac
     2221 ttcgccatga acagccctgc cgccctgatc ggtacggatg ccaacaccgc cgccgaagtg
     2281 attaccacca agctgcaatt ccctgtcgat gcggcaaccc tgttgcatac cgctaaggaa
     2341 gtgggcgaaa acaccatcct ttcccgtgca ggcggtgcgc caaccctcgc agtcggtatg
     2401 gcgcacatta tgagccgcct gattccgggc gaggcgatga tggcgttctg gtatcacttc
     2461 gccctattgt ttgaagcctt gttcatcctg accgccgtcg atgccggtac gcgcgtcgca
     2521 cgttttatga ttcaagactt gggcagcatc ttctacaaac ctttcggcaa caccgactcc
     2581 atccccgcca acctgattgc gaccttcttc gccgtggcat tgtggggcta cttcctctac
     2641 accggcgtaa ccgacccgct gggcggcatc aactcgctct ggcctttgtt cggcatcgcc
     2701 aaccaaatgc tggcaggcgt ggccttgatt atgtgcgccg tggtgctgat taagatgaaa
     2761 cgcgaccgtt atgtttgggt ggtactcgtt cccgccgtcg gcgtactgtt cgtaacctgc
     2821 tacgccggcc tgcaaaaact gttccacagc gacccgcgca tcagcttcct tgcccacgcc
     2881 ggcaaataca gcgacgcatt ggctaaaaac gaagtccttg cgcctgccaa agacatcggc
     2941 gaaatggcgc aaatcatctt caacgacaag attaatgccg gtctgaccat cctcttcttg
     3001 tcggttgtcg tgattgtcgc cgcgtacggt ttgcgtaccg ccctcaaagc acgcaaagtc
     3061 ggctggccga ccgccaaaga aatcccggcg gtgtaccgcg acggcaagca gccggaggca
     3121 caaagtgaag cataagctcg cgtcttggtg gaaaaccatc aagctgacgg caaacttgat
     3181 ggcaggcgtg cctgattatg aaaactacgt tgcacagcag cgcaaacata atcccaacgc
     3241 ccccgtgatg accaagctgc aatttcaaga ctactgccgc aagcgccgtt gcggcgcaaa
     3301 cggcggacgc tgctgttaag cctgcttgaa acaaattccg tctgaacgcc gcttcagacg
     3361 gaattattat a
//
LOCUS       DAWFAN010000176         3271 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWFAN010000176 DAWFAN010000000
VERSION     DAWFAN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.176"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(63..2234)
                     /locus_tag="WGO65_001824"
     CDS             complement(63..2234)
                     /locus_tag="WGO65_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0328077.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNAKAVLDTITVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKEYEAGKGFRNFNGGKT
                     VPYSALDKRSYLAKIGTSFGDGDHRIVLSHMKDQHRGIRTVREEFTVGDASSRTNITR
                     QAPSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVVGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFEISGKKKDPKDPKKE
                     IDKTDEEKAKDKKDMDLVHSYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVK
                     THDGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIAD
                     GTKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIK
                     NHGYELGASYRTGGLTAKVGVSHSKPRFYDTHPKKLLSANPEFGAQVGRTWTASLAYR
                     FQNPNLEIGWRGRYVQKAVGSILVAGQKDRNGKLENVVRKGFGVNDVFANWKPLGKDT
                     LNVNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            2976..3122
                     /locus_tag="WGO65_001825"
     CDS             2976..3122
                     /locus_tag="WGO65_001825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328078.1"
                     /translation="MMRNGGNLSGITENERKSGQGGEAADSTDRTEPIHLVLEHLRES
                     FSLS"
BASE COUNT          726 a          758 c          923 g          864 t
ORIGIN      
        1 aaattatcag aacagatgcc gtctgaaagg ctttcagacg gcattttttc gggatgtgcg
       61 ttttagaact tgtagttcac gcccaagcgt acatcgcggc ccacgcccgg cagggtattg
      121 gtccagcgtt ggctgtgcgg atagtagaac gtgttgaaca cgttgttaac cgaaagatta
      181 acattgagcg tgtctttgcc cagcggtttc cagttggcga agacatcgtt cacaccgaaa
      241 cctttgcgta caacgttttc caatttgccg ttgcggtctt tttgacctgc caccaatatc
      301 gaacccacgg ctttttgaac ataacggccg cgccagccga tttccagatt cgggttttgg
      361 aagcggtagg caagggaagc cgtccaagtg cggccgactt gtgcgccaaa ttcaggattc
      421 gcgctcaaca gttttttagg atgggtatcg taaaagcgcg gtttgctgtg gcttacgccg
      481 actttggcag tcaggccgcc ggtgcggtag gacgcgccca attcgtaacc gtggtttttg
      541 atgtaaccgg cattgacggc ttcacggacg gcggcagaga cgtggcggtt ttgcggattg
      601 gcaagcgcgt ctttgatggt ctgccagaag tagctgccgt tggcggcaaa cgtgccgtcg
      661 ttgtagttga agccgatttc ggtattgcgc gcgcgttcgg ctttggtgcc gtcggcaatc
      721 gagatgatgc cgcgtttgcc gtgggtttgc agcgcgtcat acaggcgcgg gctgcggctg
      781 gcgtagttgt ggcttgcgct gaagctccag tgttcgtgcg gctgccaaat cacgccgaaa
      841 ctcgggttaa ggttgctgct tgaaacggtt ttgccgtcgt gggttttcac cttgaagcgg
      901 tcgtaacgca gcccgccggt cagggtaaag ccgtcaatct cgtgaatggc ttcgatatac
      961 gcgccggtat cggttttggt cgggttggac agtttgtagg aatggacaag atccatatct
     1021 ttcttgtctt tcgctttttc ttcatcggtc ttatctattt cttttttggg atctttcgga
     1081 tctttcttct taccggagat ctcaaattcg ccgttcaaaa acgcttgcgg tttgatttcc
     1141 tgatggcggt agttgatacc gtatttcaac agggtttgtt cggcaaggcg gctgtcaaag
     1201 ttgaagttca taccccgagt ggtgattcgg gtgtggttag ggccgaccac attgcctgcg
     1261 tagccgttat ctttgtcatc ggcggaatag cgtttctttt ccaacacata ggcgttggca
     1321 tccagttttt cgacaaagcc caaatctttg ccggtgtacg ccaagttggt gttggattgt
     1381 gtggtttcgc ggtaggatgg ggcttggcgt gtaatatttg tccgtgaact tgcgtcgcca
     1441 acggtaaatt cttcacggac ggtacggatg cctcggtgct ggtctttcat gtggctcaat
     1501 acgatgcggt ggtcgccgtc gccgaagctt gttccgattt tggccaggta gctgcgtttg
     1561 tccagtgcgc tgtacggtac ggttttgccg ccgttgaaat tacggaagcc tttgccggct
     1621 tcatattctt tttcatcgtt gcggttgtaa gagaacaaac cgtcgaagtt gccctctttt
     1681 ccgaatacgc tggcgccgta gcttacgcct tcgttgctgg caaagccgct gttgaggcgc
     1741 acgccccagt ttttatccaa gcctttgagc aggtcttggg catcgacggt tttggcgata
     1801 atcgcgccgt tggttgcgcc gataccggca gaggcagaac ccgcgccttt ttgtacggaa
     1861 acgactttaa ccaaagcggg atcgacaata aatctgcctt ggtggtaaag gatttggctg
     1921 tcggaatagg cgttgtccac cttgatgtcg acagagttct gacccatgcc gcgcagcgtc
     1981 aggaattggg acgtgccgtt gccgccgccg aaatcgatgg acggctcttc ttttaagagt
     2041 tcgcgcatat cggttgcggt gctttcgtct ttttgttgaa gcgtaacgat gttggtacgg
     2101 attttgctgc cttggcggtc gccttttacg gtaatggtat ccagtacggc cttggcatta
     2161 ttttctgccg cgtgggcaaa acctgccgcc agggtaagcg agagcaggct gaggcggaaa
     2221 aacggggcgt tcatttgttc gtccttttga gtgtatgaag ggaagtaaat ccaaaccgtt
     2281 aaggtttggc aggataagaa aaataataat tatttttgtt tatattaacg agagggggga
     2341 tttgtgtaaa gctgattatc gtttttattt gcgaagtgtt gttttttgtt gacaggtttt
     2401 gtcggaaatg tatagtggac taacaaaaat caggataagg cgacgaagcc gcagacagta
     2461 cagatagtac ggcaaggcga ggcaacgctg tactggtttt tgttagtcca ctataaaaaa
     2521 cggcggaaat atagtggatt aacaaatgcg ggaatgacga aaagcaacct ttccgcgtcc
     2581 ttcccgcgaa agcgggaatc tagaacccct aacgcggcag gaatctatcg gaaaaaaccg
     2641 aaaccgaacg aacctagatt cccgcctgcg cgggaatgac gaggcctgcg cgggaatgac
     2701 gaaaagcaac ctttccgcgt ccttcccgcg aaagcgggaa tctagaaccc ctaacgggca
     2761 ggaatctatc ggaaaaaacc gaaaccgaac agactggatt cccgcctgcg cgggaatgac
     2821 gaagcctgcg cgggaatgac gaaaagcaac ctttccgcgt ccttcccgcg aaagcgggaa
     2881 tctaggaact caaagctgca agaatttatc agaaacaact ggaactcaaa aaaactagat
     2941 tcccgcctgc gcgggaatga cgaagggtta aagaaatgat gagaaacggc ggtaatttat
     3001 cgggaataac cgaaaacgaa cggaaatcag gacaaggcgg cgaagccgca gacagtacgg
     3061 ataggacgga accgattcac ttggtgcttg agcaccttag agaatcgttc tctttgagct
     3121 aaggcgaggc aacgacgtac tggtttttgt tcatccacta taacagcaac cctgtcgccg
     3181 tcattcccgc gaaagcggga atccagtccg ttcagtttcg gtcatttccg ataaattcct
     3241 gctgcttttc atttctagat tcccactttt g
//
LOCUS       DAWFAN010000177         3181 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWFAN010000177 DAWFAN010000000
VERSION     DAWFAN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.177"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            72..497
                     /locus_tag="WGO65_001826"
                     /pseudo
     CDS             72..497
                     /locus_tag="WGO65_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            503..1732
                     /locus_tag="WGO65_001827"
                     /pseudo
     CDS             503..1732
                     /locus_tag="WGO65_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            1790..2410
                     /locus_tag="WGO65_001828"
     CDS             1790..2410
                     /locus_tag="WGO65_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0328079.1"
                     /translation="MTTHHVELRNVTKRFGAQKAVNQVDLVLKAGESVGLAGYNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            2499..2630
                     /locus_tag="WGO65_001829"
                     /pseudo
     CDS             2499..2630
                     /locus_tag="WGO65_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2627..3121
                     /locus_tag="WGO65_001830"
     CDS             2627..3121
                     /locus_tag="WGO65_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ0328080.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTKWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVSFDDMPDT
                     YIFK"
BASE COUNT          802 a          834 c          821 g          724 t
ORIGIN      
        1 actatattat cccgtatgcg gattggtttt aagatttgta aatttaattt gcatcaaaaa
       61 atcgccgata gatgattcat ataatatcaa tattaaagag tatcggtata tcggggatag
      121 tcatgtcctg tttttcaatc aaacgtatgt ccgcgtttcg ggcgcggata acggcgtttt
      181 ttgccgcctt tgtctttttg acggcggcac tgcccgctta tgcggagcat ctgcctgatt
      241 ttctggcgaa aatacagcct tcggaaattt ttccgggtgc ggaccgttac ggcaagccgg
      301 aaggtaagcc tatggttgcc cgcgtttaca aaggcgatga gcagttgggc ttggtctata
      361 tcacgaccga tgcggtcaat acgcgcggtt attcgagcaa accgattgat acgctgatgg
      421 cgttggcaaa cgacggaacg atagccggag cgaaattgat tgatcaccat gagtcgatta
      481 tgctgatcgg tatcccgcat ttgcccgcgc ccgggcgggc gatacgctca aactggcttc
      541 cggcgtatat caaaccaaac tttatatcga caaaccgatt acgattgaag gacctgccga
      601 ccgttccgca accatcgaag gggacaggag cggtcgtacg atagctgtgc atgcgccgga
      661 cgtaacgctc cgcaacctga ccgttacacg ttccggtatg agcctgccag caatggatgc
      721 cggtatttat ctcgaagaaa ccgccccacg cgccctgatt gaacacaaca atatttttga
      781 taattcggtc ggcgtatatc tgcatggttc tgccgaagcg atggtgcgcg agaataaaat
      841 cgtcggcgac gcgactttgc gcgtgaacga gcgcggcaac ggcgttaccg tttggaacgc
      901 acccggtgcg caggtcgtcg gcaacgacat ttccaaaggg cgggacggca ttttttccaa
      961 caccagcacg cacaacacct ataaaaacaa ccgcttcagc gatttgcgtt tcgccgtcca
     1021 ctatatgtac accaacgaca gcgaagtcag cggcaatatt tccgtgggca acaatatggg
     1081 ctatgtgctg atgttttccg aacggctcaa agtattcgac aatattgccg tcggtagtcg
     1141 cgactagggc atcatgctca actatgtcaa ctattccgat attcacgaca atattatcaa
     1201 caaagcgggc aagtgcgttt ttgcctacaa tgccaactac gataaactgt ccgccaatca
     1261 ttttgaaaac tgccaaatcg gcatacactt taccgccgcc atcgaaggca cgtctctgca
     1321 cgacaattcc tttatcaaca acgaaagcca ggtcaaatac gtcagcacgc gctttctcga
     1381 ctggagcgag ggcggacacg gcaactattg gagcgacaac agtgcgttcg atttgaacgg
     1441 cgacggcttc ggcgacagcg cgtaccggcc caacggcatc atcgaccaaa tcatctggcg
     1501 cgcgcccgta ttgcgcctct tgatgaacag tctcgcaatc agcatcgtca aatgggcgca
     1561 ggcgcagttt cccgccgttc tgcccggcgg cgtggtggac agcaaaccgc tgatgaagcc
     1621 ttatgtcccc aaaattcaaa cccgctatca ggcaatgaag gacgagttgc tcaaagaagc
     1681 cgaaacacgg cagtcggaat ggggcagggc ggaaaacggt tctttgaact agtctgcttc
     1741 agacggcatc cggatccaaa tgccgtctga aaacacaaaa ggaacaacca tgaccacaca
     1801 tcatgtcgaa ttgaggaatg tcaccaaacg gttcggggcg caaaaagccg tcaaccaagt
     1861 cgatttggtt ttgaaggcag gagaaagcgt cgggcttgcc ggatacaacg gcgcgggcaa
     1921 gtccaccatt atgaagctga tactcgggct gattaccccg accgaaggcg aagtgatgct
     1981 tttgggcgaa cgtaccggca gcaaagcggg ggcgcggctt cgcagccaaa tcggctacct
     2041 gcccgaaact gttgcgttgc acccttcgct gaccggcatc gaaacgctgg atttttatgc
     2101 caaacttaaa aaacagccgc tcacgcagaa tcgggggctg ctcgagcgcg tcggcatttc
     2161 acaggcggca caccgccgcg tcggcactta ttctaaaggg atgcgccaac gccttgcctt
     2221 ggcacaagcc ctgctgggcg agcccaaagt cctgctgttt gacgaaccga caaccggcct
     2281 cgaccctgca tcaaggcaaa tgttttacga agtcgtgcgc gaactcaacg ggcgcggcgc
     2341 gaccgtattg ctcagtaccc acgcccttgc cgagttggac ggacacgccg accgcattat
     2401 cgtggattaa atttaaacca gtacggcgtt acctcgcctt accgtactat ttgtactgtc
     2461 tgcggcttcg ttgccttgtc ctgatttttg ttaatccact atatgcgggt atggcgggtt
     2521 tgagcggaca aatcagcctg accgtccccg ttttgctgac cgctcaggtt ttatgggtta
     2581 tcattccgct tgttttggca gccggaattt ttagaaagcg acaaatatga aaaaaaccct
     2641 gttggcaatt gttgccgttt ccgccttaag tgcctgccgg caggcggaag aggggccgcc
     2701 gcctttaccc cggcagatta gcgaccgttc ggtcggacac tattgcagta tgaacctgac
     2761 cgaacacaac ggccccaaag cccagatttt cttgaacggc aaacctgacc agccagtttg
     2821 gttctccacc atcaagcaga tgttcggcta taccaagctg cccgaagaac ccaaaggtat
     2881 acgcgtgatt tacgttaccg atatgggtaa tgttaccgat tggacaaatc ccaatgccga
     2941 cacgaagtgg atggatgcga aaaaagcctt ttatgtcatt gacagcggtt ttatcggcgg
     3001 tatgggtgcg gaagacgcac tgccgttcgg caacaaagag caggctgaga aatttgcaaa
     3061 ggataaaggc ggtaaggttg tcagtttcga cgatatgcct gatacctata ttttcaaata
     3121 aatccaaaca aaaatgccgt ctgaacgttg aagccaaggt tcagacggca ttttttatag
     3181 t
//
LOCUS       DAWFAN010000178         3179 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWFAN010000178 DAWFAN010000000
VERSION     DAWFAN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.178"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(237..1094)
                     /locus_tag="WGO65_001831"
     CDS             complement(237..1094)
                     /locus_tag="WGO65_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ0328081.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADSAPLTDYLARRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(1156..1971)
                     /locus_tag="WGO65_001832"
     CDS             complement(1156..1971)
                     /locus_tag="WGO65_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0328082.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            2035..2205
                     /locus_tag="WGO65_001833"
     CDS             2035..2205
                     /locus_tag="WGO65_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328083.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            2215..3000
                     /locus_tag="WGO65_001834"
     CDS             2215..3000
                     /locus_tag="WGO65_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ0328084.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLSKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDAWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT          708 a          784 c          905 g          782 t
ORIGIN      
        1 gcacttgaaa tccgaatcca agttattctt cattgtattc tacctggggt cgtagttatt
       61 aaattaccat acttatttgt ttggatcgtc ataatattag ttggcatgtt gttgttatat
      121 ttatcatatc caatatcctt attaactcgg atgactcttt cataaattgt atgggaaatg
      181 agatttccat cactaagcct ctcaatagat atacgcgtta atgttgggaa agggtttcag
      241 acggccttca taatgcgttc gatttcgtgc caaaagccgt ccggccgaca ttccaaaacg
      301 gcaatcaagc cttggtcgat acggcggatt tcggttgcgt tcaatgtttc ggtttcggcg
      361 acacgcgcag gcgcgaggta ggccatacgg tcgagcagat ggtagcggac ttcttggcgt
      421 ttaaacccgt attccgccca atcttgaata tagatgccgc gccaaccgta tggattaagc
      481 ggtggctcaa aagcatggaa accgtgtgga aaaaatccga tgccgcgcac gatggaaacg
      541 ggttttacgt caagcggcag accgtgttgc cgcaaggttc ggatgccgtc tgaagtgtgc
      601 ggcagaccga gttgggcggt cagtttggcg gcttttccca acagcgtgtc tttggggtcg
      661 agtccgcgca tgtcttcgag cctgcccgtg ccgccgccgt aatatttgca ggtcagctcg
      721 atatggtagg gcttgccgtt aagccttacc acaaaatccg ccgcgccttg cgtattgccg
      781 tccgaaccgg aaaccgtgag gttgtgcgcg agcagttcgg cgtgtggcgc gttggcaaac
      841 caaaatgcca gcagttcttc ggcgtaaatg cccaagcggt gtccgaacgg ggcgcgtcgg
      901 gcgaggtaat ccgtcagcgg agcggaatcg gcatccaatg ccaaaagata gcggaaaccg
      961 cgttccccca atagttcgcg cacgcccaat tcgcaaccgc tttgccacaa aggcggcgca
     1021 gtcagcagcg aggcgaggtc gcggacgggt tggctggtga gcttccacca taatgcgtct
     1081 agggcgtaat tcatgcttat ccgaatatca gtcatgccgt ctgaaaagct ggaaatgttt
     1141 tcagacggcc ttaaatcagt cttttttatg gatgcgatgg ctgacggtat tgaaaaaacc
     1201 gcgcaggatg tcgatgtctt cggtttgcgt gttggcgcgt ccgaacaggc tctgcatacg
     1261 gcgcatcaga cgctcgccgt tgcggcggtt gaaaaagccg atgtcgttca tcacgctttc
     1321 catgtgggcg accatgcctt tgatttgctc gtgggtcgca gcgtggtctt cttgttgaag
     1381 atgggtcatg ggcgaaccgg tttggctgaa gatttcgtag cacacgacct gcacggcttg
     1441 ggcgaggttg agcgagaaat agtcgggatt gccgttgatg gtcatcagtc ggttgcaggc
     1501 ttggacttct tcgatgctca agccgaaagt ctcgttgccg aaaaccagtg ccactttctc
     1561 gcctcggttt gcggtctgca gtaattcgga taccaaatcg cgcggggttt gcagcggcgc
     1621 agtaatttcg cggcggcggc tggtcagggc gcaggcgatg gtggtgtcgg caagggcttc
     1681 gtccaaagaa gcggcaatgg tggcattttc caaaacgtct gccgcgccgg aagcgaggat
     1741 gaagctttct tccggtaatt taaacgattg aggatgctcc gggtcaaaca cgggcgggtt
     1801 ttccgtcatc ggcgttgcca tcagatttgg ggcgacgatg gtcagtttgt gcagacccat
     1861 tgttttcatc gcgcgcgcgg cagagccgat gttggcggga tggctggtgc gcgtgaggat
     1921 gatgcggatg ttgtccagat aagcgggcag ggcgggtttg agagtagtca tgttcggttt
     1981 tcggtcggaa tggaataggg tataatgcgc cgtctttttc gttcggacgg taaaatgccg
     2041 tctgaaatgt tctttaacaa cgtcggaaat ttaacgttgc gcctgaagca gacacgcgtc
     2101 gggcggtatt ttacccgatt tggcaggatt taccccaacc aaataaactc cgattttgcc
     2161 gctccgaagg actgcgcgtc cgaaccggcg gtatgccacc cttaaggaaa tctgatgaat
     2221 ccgtttttga atacagcctt taaagccgcc cgccgtgccg gtcagatgat gatacgcgcc
     2281 gcaggtaacc tcgatgccgt caaaaccgac agcaaagcct tcaacgattt tgtttccgat
     2341 gttgaccgca attccgaaat catcctggtt gaggctttga aagaagccta tccgcaccac
     2401 aaaatcactt gtgaagaaag cggctcccac ggcaaagccg cagccgagta cgaatggatt
     2461 atcgatccgc tcgacggcac gaccaatttc cttcacggtc atccccaata cgccatctct
     2521 atggcgctcc tgcacaaagg cgtgttgcaa gaagctttgg tttacgcccc cgaacgcaac
     2581 gacgtatata tggcttcgcg cggcaaaggc gcgttgctca acgaccgccg catccgcgtt
     2641 tccaaccgca tcgaattgaa ccgctgcctg atcggtaccg gcttccctgt tgtcgatcaa
     2701 agcatgatgg acaagtatct ggcgattttg aaagatttct tgtcaaaaac cgccggcggc
     2761 cgtcgtgaag gcgcggcttc tttggatttg tgtgctgtag caacaggtcg ttttgacggc
     2821 tttttcgagt ttaacctcaa accgtgggac attgccgcag gtgcattgat tgttcaggaa
     2881 gcgggcggta tcgtaaccga tatgacgggt gaggacgctt ggttggaaag cggcgatatt
     2941 gtcgccgcca atcccaaagt attggcgcag atgctgaaaa tcatttccgc acacgtttaa
     3001 atttcagccc tttcccgaaa atatgggaaa acgggcgggt atgccgtctg aagtatcgcg
     3061 ccgatatttc agacggcatt tttattggca acgcggcagg acggcggaaa cggtcaaatg
     3121 ccccaaactg ccgaggatgg aaacccatat ctgattaaac ggagaaccct gtgcgattt
//
LOCUS       DAWFAN010000179         3170 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWFAN010000179 DAWFAN010000000
VERSION     DAWFAN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.179"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(57..1157)
                     /locus_tag="WGO65_001835"
     CDS             complement(57..1157)
                     /locus_tag="WGO65_001835"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ0328085.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPGR
                     LRRKLLDLQDTGADELHRSRMLSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHSYSVQLADLPLLAERTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGEKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLAKCFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1482..3056
                     /locus_tag="WGO65_001836"
     CDS             1482..3056
                     /locus_tag="WGO65_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ0328086.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          655 a          795 c          924 g          796 t
ORIGIN      
        1 aaagaggaaa aaatgccgtc tgagcggcgg aacgggcttt cagacggcat ttgcgctcaa
       61 taataatatc ccgcgcccag aatacacggt ttggatgcgc cggttgcttt gtgcggacta
      121 ccggggatgc gattaatcca acacgccgcc aaccacgcaa atgcggcggc ttctacccat
      181 tgcggatcga ggttcagttc ggcggtgctg tgcagggaaa cgcgtgtgcc gaagcatttt
      241 gccaaatccg ccattaaaac aggattgcgg atgccgccgc cgcaaatgta catttgacgg
      301 gcatctgccg ctgcgtgtga gacggcgtcg aaaacggttt gcgcggtaaa acgggaaagc
      361 gtccgtaata cgtcgtatcg gttttcgccg ccgtcaaggt aggtttcgag ccaatttagg
      421 gcaaacagtt cgcgccccgt gcttttgggg tggggttgtg cgaaatatgg gtgggcgagc
      481 agcctgtcga gcagttgcgg caatatgttg ccttgtgccg ctttttcacc gtttttgtcg
      541 taaggaagct gccagtgtgc ctgcgtccac gcgtccatca gcatattgcc cggccctgtg
      601 tcgaagccga aggcgggtgc gtcggggggg agtacgctga tgttggcaat cccgccgatg
      661 ttcagtacca cgcgtgtttc tttgtcatcg cggaacaggg ctttgtgaaa ggcggggacg
      721 agcggcgcac cttgtccgcc ggccgcaagg tcgcggctgc ggaagtcgcc gacggtaaaa
      781 atctgagtcc gttccgccag cagcggcaaa tcggcaagct gtacgctgta actgtgttcc
      841 ggcgcgtgtc tgacggtttg cccgtggcag ccgagggcgg taatgtcgga cggcgcgagg
      901 ttttgactgc acagcagttc ggcggcggtt tgcgcgtaca ggcggctgag ttcttgcgac
      961 aacatcctgc tgcggtgcag ttcgtccgcg cctgtgtcct gcaaatccag caatttgcgg
     1021 cgtaacctgc cggggtaggg ggtaaaggcg tgcccttccg cgcccagcca tttgccgccg
     1081 tccatccgta ccagcacggc atccgcccca tccatactgg ttcccgacat gatgccgatg
     1141 taaagctgtg tttccatcat cactcccaaa ctggtgcaaa acgccatttt aacgtgtatt
     1201 gacgctcgta tactgatttg ccgccgcagt gtaaataaag tgtaaataaa tgtttcaaga
     1261 cggatggaaa aatattataa tgcgcccgca acatccagta gtagaagtgt catacaaacc
     1321 gtttccggca gcagttttgc attcggtcag gtttgggggt attcggatgc ggttcagaag
     1381 gaaggatgcg tctgccatat cccgaaacgg cagttcgacc ggaggcagca gtacagtgtc
     1441 ggcaacactc atgatttcca ccacattaaa ggaagattgc catggctcaa atccaaatga
     1501 gcgcaaatgt taaaaccatc aacgccgtct ttgccgccat gctggtaggt acagtcggct
     1561 attttattta ttggggcttg ggttataccc attacaatta cgccgcctta ttcattattg
     1621 ccacgatgtt cggcgtgttt atggcgttca acatcggcgg caacgatgtt gccaattctt
     1681 tcggcaccag cgtcggtgcg ggtacgctga ccatcccgca ggctttgctg attgcggcgg
     1741 tatttgaggt cagcggcgcg gtcatcgcgg gcggtgaggt aaccaatacc atacgcaaag
     1801 gcatcgtcga tttgaagggt gttgatttcg aacccataca gtttgtgttt attatgatgt
     1861 ccgcgctttt ggcggcggcg ttgtggctgt tgtttgcctc gaaaaaaggg cttccggtat
     1921 ctaccaccca ttccattatc ggcggcattg tcggcagcgc ggtatgtatg gcggtaatga
     1981 acgatgccgc atcgggcgat ttgatacgtt ggggcaagct gggcggtatt ggtgtttctt
     2041 gggtattgtc gcccgtgttg ggcggcgcgg tgtcctattt cctgttttcg cgcgtcaaga
     2101 aaaacgtctt agattacaac gcttgggcgg aaggcacgct caagggcatc aagcaggaaa
     2161 aaaaggccta taaagaacgg caccgcctgt ttttcgaggg tttgtccgaa gccgaaaaag
     2221 tcgagtacgc caccaaaatg gcgcacgacg cgcaaattta cgacgaaccc gaattcgatc
     2281 cgcaagagct gcaatcggag tattaccgcg gtctttatgc gttcgacaac cgtaaaaaca
     2341 atgtcgattc ctacaaagcg ctgcattctt ggattccctt tatcgcttcg ttcggcgcga
     2401 tgatgatttc cgctatgctg attttcaagg gcttgaaaaa cctgcatttg gggatgagca
     2461 acgtcaacag cttcctgacc atctttatga taggcgcggc ggtgtggatg gggacgtttg
     2521 tgtttgccaa aagcctcaag cgtaaagact tgggcaaatc gacctttcag atgttttcat
     2581 ggatgcaggt ctttaccgcc tgcggcttcg cattcagcca cggtgcgaac gatatcgcca
     2641 acgccatcgg tccgtttgcc gcgattatgg atgttttgcg taccaacagc gttgccgcgc
     2701 aaaatgtcgt ccccccgatt gcgatgctga ctttcggtat cgcgctgatt gtcggtttgt
     2761 ggtttgtcgg taaagaggtg attaaaaccg tcggtacgag tttggcggaa atgcatcctg
     2821 cttcgggttt taccgccgaa ctgtccgccg catccgtcgt gatgggcgcg tcgctgatgg
     2881 ggctgcccgt gtccagtacg catatcttgg tcggcgcggt actcggcatc ggtctggtca
     2941 accgcaatgc caactggaaa ctgatgaagc ccatcggttt ggcgtgggtc attaccctgc
     3001 ctgccgccgc cgtattgtcg gttgtctgct acttggtttt acaggcagta ttctgattgt
     3061 aaaatactga tgccgtctga acccgtgttc agacggcatt ttgttgatgg aatgtgcggg
     3121 cttgtgcctt atgcacaatc tgttctgtcg ggatatgccg tttggtatag
//
LOCUS       DAWFAN010000180         3148 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWFAN010000180 DAWFAN010000000
VERSION     DAWFAN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.180"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            98..796
                     /locus_tag="WGO65_001837"
     CDS             98..796
                     /locus_tag="WGO65_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0328087.1"
                     /translation="MVRTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNRIHPFYSDSKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPTPLKAYNLTVADWHTYFVKSSQAETEGVWVHNDCPPYKRPNNATTKAQRESVQGKP
                     CVEYGKLAEKMIADHKKPLVVEYYETGTIDKSKMRAIESVQPQCPTCSAKQGGRLSQY
                     SKEQKRKLSNGNKK"
     gene            780..1133
                     /locus_tag="WGO65_001838"
     CDS             780..1133
                     /locus_tag="WGO65_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328088.1"
                     /translation="MGIKNEQIIICKKYNTEIYPISDVSKIGVAENVKQANLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1123..1446
                     /locus_tag="WGO65_001839"
     CDS             1123..1446
                     /locus_tag="WGO65_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ0328089.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1505..1732
                     /locus_tag="WGO65_001840"
     CDS             1505..1732
                     /locus_tag="WGO65_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328090.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            1860..2402
                     /locus_tag="WGO65_001841"
     CDS             1860..2402
                     /locus_tag="WGO65_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0328091.1"
                     /translation="MINHIEKYTGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKHDLNYKSRSEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVVYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVGNLSDMNRKPFTW"
     gene            2483..3019
                     /locus_tag="WGO65_001842"
     CDS             2483..3019
                     /locus_tag="WGO65_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0328092.1"
                     /translation="MDYNQTVLSHLQNFWKHHDIKGFTWALGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDRFQLGKTVN
                     IGRPWVEQSSFQHFLISLPYPYGQELEYMDNVRFFWLLPITQPERLFLNTHSVEDLET
                     RFDELGIDYLDINRASVI"
BASE COUNT         1016 a          578 c          636 g          918 t
ORIGIN      
        1 gtgtttaatt ttcaggcgac cttcccctta tttgaaacag gatattggga actaagttct
       61 tcaaaaatcc tacacctgct ccttccacgg cagcaccttg gtcagaacgg cagacggcta
      121 caaagccatt gcccatattc aagccggcga ccgcgtcttc gccaaagatg aaaccagtgg
      181 aaaaactgga tacaaacctg ttaccgcccg atacggcaat ccgtaccagg aaaccgttta
      241 cattgaaatt tcagacggca tcggcaacaa ccaaaccctg atttccaacc gcatccatcc
      301 gttttattcg gacagcaaat ggattaaagc ggaagatttg aaagcgggaa gcaggctgtt
      361 atccgaaagc ggcaaaaccc aaaccgtccg caacatcgtt gtcaaaccaa caccgctcaa
      421 agcctacaat ctgaccgttg ccgactggca tacctacttc gtcaaaagca gtcaggcgga
      481 aacggaaggg gtttgggttc ataatgattg tccgccctat aagagaccaa ataacgcaac
      541 gacaaaagca caacgagaaa gtgtacaagg aaaaccctgc gttgaatacg gtaaattagc
      601 cgaaaaaatg attgccgacc ataaaaaacc attagttgtc gagtattatg aaacagggac
      661 aattgacaaa tctaagatga gagcaattga atcagtacaa ccgcaatgtc cgacttgttc
      721 tgcaaaacaa ggggggagat taagccaata ttcaaaagaa cagaaaagga aactgagtaa
      781 tgggaataaa aaatgaacaa ataataattt gtaaaaaata taatacagaa atatatccta
      841 tttccgatgt ttcaaaaatc ggtgttgctg aaaatgtcaa acaggcaaac ctgtatccga
      901 ttaacgggtt aaggcaccgt cccaaaggtg atactaacgg ttggtatatc tgggccggtg
      961 aaaacttctc ctatgataaa aattttttcc tgcccttgca tactttccat ttgcaaatat
     1021 ggcgacccga aatcatcccc tttttgacac ttccgcccgg ttaccgtttt ttaattgggg
     1081 aaaacggtta tgaagacgta tggtttgatg agttgctatt aaatgataat tgacgaaaaa
     1141 tctacttcag atgtactgaa acaatatcaa gaactagggt ttgatttgac aaaacctatg
     1201 ataatagagt tttttatagg aggttctcaa aagaatctgc aatcaataga aagccggctc
     1261 atttcttatc ggcaagattt tcaaatcagt attgaacaag atgaattcgg agaaatgtgg
     1321 acttgctatt gttccgtcaa tatcgtccct tcccttgaaa acattttagc tattgaaact
     1381 acgttatttg gtattgcaca aaaaaatgat tgtaattatg aaggttttgg ttcgtatggg
     1441 aactaacaaa aagtgtaggt tgggttgaaa aaaccatatt cccattaaag attagggtgt
     1501 gtctatggac ataaaattca atactttggg ggttatcctg aatggggtca atcctgaaga
     1561 aaaattcata aaaattattg atgatcagga aaataccggg ggatttctga tactgttatc
     1621 ttcaaatgac aaattctcat catttgacag ttacgatgac tgggtcgaaa acttagaaat
     1681 attaaaagaa tacctgcaag aatctcattg gataataaag tgggtcggat agtcggcaaa
     1741 cgtagtccgt ctgaaacgct tttacttttc agacggactt tctatcaatg ggaacaaggc
     1801 gttttgtctc aatctggtct atgattattt cgatacagtg atgggaagct aagaaaaata
     1861 tgattaatca tattgaaaaa tatacaggca ctatttctca tggctcgaaa agtgattcgg
     1921 gaagtcagta caaattaaat atcgcagcta ttccctcttc ccctaacagg gatttaaaaa
     1981 cttatattac tttagggtta agcaaacatg atctgaatta taaaagcaga tctgaaatac
     2041 tttttgtatg ttctttaaaa tatgatgaaa atcagatttt tccattttta agatggttgg
     2101 cagaaaccat cattgagaat aaaaaaatcc ttcttcgggg gcaggttgtt tatttgccca
     2161 gaagcattgt tgattcaaca aaaatggatg cactgtatgt ttccgcccca ttttatttcg
     2221 atgatgattt tcaggtctgt tatggcgaac actacaatat cgttttccct ttgcttgtcc
     2281 ccttgtataa acaggaagca gaattggtgg aaaagaaagg ttggaatgct tttgagcagt
     2341 tcttgttgga taatgaagtt ggcaaccttt cggatatgaa tcggaaaccg tttacttggt
     2401 aaataccttc ttcaaacggt caatgccgtc tgaaaaacct tgtttcaggt ttcaggcgac
     2461 ctttgaaaac aggctaaaaa gcatggacta taaccaaact gttttatctc atttgcaaaa
     2521 tttttggaaa catcatgata ttaaaggatt tacttgggct ttaggaagga ttgttgaaga
     2581 acttcccgat ttccaagttt tccaagtgat tcctaatcat gaagatgagc cttgggttta
     2641 tgtcagctct ggaataggtc aatttttagg gcaggagttt tttatcatca gcccatttga
     2701 aacacctgaa catattgaga ccttggcaat gctggcatct gcaagtatgc attatcccga
     2761 tcgatttcaa ttagggaaaa cggtcaatat cggcagaccg tgggttgagc aatcatcctt
     2821 ccagcatttt ctgatttctc tgccttatcc ttatgggcaa gaattggaat atatggataa
     2881 tgtccgattt ttctggttat tgccaattac ccagccagaa agacttttct tgaatactca
     2941 ttcagtagaa gacttggaaa cgagatttga tgagctaggt attgattatt tggatataaa
     3001 tcgtgcaagc gttatttgat atttatgttg tttgaaatct ttcagcaagc gtaggttgga
     3061 ttggaaatcc aacaatcaaa atgccgtctg aaaccgtgtt taattttcag acggcatttc
     3121 cttcatttga aataggatat tgagaact
//
LOCUS       DAWFAN010000181         3137 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWFAN010000181 DAWFAN010000000
VERSION     DAWFAN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.181"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            61..2319
                     /locus_tag="WGO65_001843"
     CDS             61..2319
                     /locus_tag="WGO65_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ0328093.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALSGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPYDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGTLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLDGSHTAALEK"
     gene            2332..2997
                     /gene="pgmB"
                     /locus_tag="WGO65_001844"
     CDS             2332..2997
                     /gene="pgmB"
                     /locus_tag="WGO65_001844"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ0328094.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGISIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVSEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLET
                     LKANGKKIALASASKNGPFLLERMGLTHFFDAIADPAAVAHSKPAPDIFLAAAEGVDA
                     DIRQCIGIEDAVAGVAAIKAAGALPIGVGKAEDLGSDIALVSGTAELTYAYLQNVWAQ
                     SGR"
BASE COUNT          809 a          944 c          790 g          594 t
ORIGIN      
        1 tgttaatcca ctataagatt tcaccattcc ctcaaatcaa tccaaacagg agcttcataa
       61 atgtacacaa gaattatgga aatcagccct tggacgctgc gttcggcaaa gctggaaaaa
      121 gaacacaaac ggctgcaaga gagcctgacc agtttgggca acggctatat gggtatgcgc
      181 ggcagctttg aggaaaccta ttccgccgac agccacttgg gtacctatat cgccggtgtg
      241 tggtttcccg acaaaacccg cgtcggctgg tggaaaaacg gctaccccaa atatttcggt
      301 aaagccatca acgcgctcaa tttcagcaaa gtcaaaatct ttgtcgacgg acaagaggtg
      361 gacttggcga aaaacgatgt tgccggcttc tccgtcgaac tcgatatgca gcacggcgtg
      421 ttgcgccgct cgttcaccgt attcggtgtg cgtttcaatg tgtgcaaatt cctgtccgtc
      481 gcgcaaaaag agctggcggt tatccgctgg gaagccgtat ccgttgacgg caaaacccac
      541 caagtccgca tcgattccat catcgatgcc gacgtgaaaa acgaagactc caactacgaa
      601 gaaaaattct ggcaggtatt ggacaaaggc gtttcagaca gtctctccta cattgccgcc
      661 caaaccgttg ccaacccttt cggcgtggaa caatttatcg tcaacgccga gcaaactttc
      721 gccggcagct tcaaagccct cagcggcagc caaaccgact ggcaagtctc caattctttc
      781 gaggtcgaag tcggcggcac gcccaaaacc tttgaaaaac gggttattgt taccaccagc
      841 cgcgattatc agggcttgga agcagtgaaa gccgcaggcc gcgccttgtc ggaaaaagtc
      901 gcaggcgttg cgtttgaaac cttgctggac gcgcacaaag caggctggct gcaccgttgg
      961 gaaatcgccg acgtggtcat cgaaggcaac gacgaagcgc agcaaggcat ccgtttcaat
     1021 ctgttccaac tgttctccac ctactacggc gaagacgcgc gtctgaacat cggcccgaaa
     1081 ggctttaccg gagaaaaata cggcggcgcg acctattggg acaccgaagc ctacgccgtg
     1141 ccgctctacc tcgcactggc ggaacccgaa gttacccgca acctgctgca ataccgccgc
     1201 aaccaactgc cacaggcgca acacaacgcg cgcgaacagg gcttggcggg cgcactctat
     1261 ccgatggtaa cgtttacggg catcgagtgc cacaacgaat gggaaatcac cttcgaggaa
     1321 atccaccgca acggcgcgat tccttacgcc atctacaact acaccaacta caccggcgac
     1381 gagggctatc ttgccaaaga aggcttggaa gtcttggtcg aagtgtcccg cttctgggcg
     1441 gaccgcgtcc acttctccaa acgcaacggc aaatacatga ttcacggcgt aaccggcccg
     1501 aacgaatacg aaaacaacat caacaacaac tggtacacca acaccctcgc cgcatgggta
     1561 ttggactaca cccgcgaagc cctggcgaaa tacccgcgtc cggatttaaa cgtgcgtgcc
     1621 gccgagttgg aaaaatgggc ggacatcagc gcgaatatgt accgtccgta tgacgaagaa
     1681 ctcggcgtat tcgtacaaca cgacggattc ctcgacaaag acatccgccc cgtgtccgcg
     1741 ctttcgcccg acgatttgcc gctcaaccag aaatggtcgt gggacaaaat cctgcgttcg
     1801 ccctttatca aacaggcgga cgtattgcaa ggcatctatt tcttcggcga ccgtttcaat
     1861 atcgacgaaa aacgccgcaa cttcgacttc tacgaaccga tgaccgtgca tgaaagctcg
     1921 ttgtcgccgt gtatccactc tattctcgcc gccgaactgg gcaaagaaga aaaagccgtg
     1981 gaaatgtacc agcgcaccgc ccgcctagac ttggacaact acaacaacga caccgaagac
     2041 ggcctgcaca tcacctccat gaccggctcg tggcttgcca tcgtccaagg tttcgcccaa
     2101 atgaaaacct ggggcggcac actcagcttc gccccgtttc tgccgagcgc gtggacaggc
     2161 tacgccttcc acatcaacta ccgaggccgt ctgattaaag tcgccgtcgg caaagaaaac
     2221 gtcgtcttca ccctgcttaa aggcgaaccg ctcgatttgc aggtgtacgg caaagacatc
     2281 acgctcgacg gcagccatac cgccgctttg gaaaaataag gagggcgcaa aatgacgttt
     2341 accgctgtgc tcttcgatct cgacggtgtc atcaccgata ccgccgaata ccattaccgc
     2401 gcatggaaaa agctcgccga agaactgggc atcagcatcg accgcaagtt taacgagcag
     2461 ctcaaaggcg tgtcgcgcga cgattcgctc aaacgcatcc tcgcgcacgg cggcaaaacc
     2521 gtcagcgaag ccgagttcgc cgaactgacc cgccgtaaaa acgacaacta cgtcgagatg
     2581 attcaggcag tcaaacccga agacgtgtat cccggcattt tgcccctgct ggaaacattg
     2641 aaagcaaacg gcaaaaaaat cgccctcgcg tccgccagta aaaacggccc gttcctgctc
     2701 gaacgcatgg ggctgaccca cttcttcgac gccattgccg accctgccgc cgtcgcgcat
     2761 tccaaacctg cccccgacat cttcctcgcc gccgccgagg gcgtggacgc agacatccgc
     2821 caatgcatcg gcattgaaga cgccgtcgcc ggcgtcgccg ccatcaaagc cgccggcgcc
     2881 ttgcccatcg gcgtgggcaa agccgaagac ttgggcagcg acatcgcgct ggtgtccggc
     2941 accgccgagc tgacctacgc ctatttgcaa aacgtgtggg cacagtcggg caggtaaaat
     3001 gcgtcagata aagtgtcaag gaagtaaaag gctgtctgaa cagtgtttca gacggccttt
     3061 ctgcttttat agtggattaa caaaaaccag tacggcgttg cctcgcctta gctcaaagag
     3121 aacgattctc taaagtg
//
LOCUS       DAWFAN010000182         3125 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWFAN010000182 DAWFAN010000000
VERSION     DAWFAN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.182"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(549..1133)
                     /gene="rnhB"
                     /locus_tag="WGO65_001845"
     CDS             complement(549..1133)
                     /gene="rnhB"
                     /locus_tag="WGO65_001845"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ0328095.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDTLAEMIKDQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLVVHPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1201..3096)
                     /gene="mnmG"
                     /locus_tag="WGO65_001846"
     CDS             complement(1201..3096)
                     /gene="mnmG"
                     /locus_tag="WGO65_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ0328096.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTYEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
BASE COUNT          558 a          753 c          964 g          850 t
ORIGIN      
        1 gtcattcccg caaaaacaaa aaaatcaaaa acacaaaact gaaatatcgt cattcccgcg
       61 caggcgggaa tctaggtctg tcggtacgga aacttatcgg gaaaaacggt ttttccaacc
      121 ctgagactcc ggattcctgt tttcgcagga atccggtttt ttgagtttca gtcattttga
      181 taaattcttg cagctttggg tttctagatt cccgcttttg cgggaatggc ggtttggaag
      241 ttacctgaaa ttcaaaaaaa cggaaaccgg acggactgga ttcccgtttt cacgggaatg
      301 acggatttta ggtttctttt ttgattttct gtttttcgcg ggaatggcgg tttggaagtt
      361 acctgaaatt caaaaaaaaa aaaacggaaa ccggacggat tggattcccg cctgcgcggg
      421 aatgacggat tttaggtttt tttttgattt tctatttttc gcgggaatga cggtttgggt
      481 tctttctctt tggagttgcg atgccggaaa tgccgtctga aggcttcaga cggcattttt
      541 gtgccggttt aaaacaaggc ctgctgcgcg agcaggtttc tgacgggggc gaagtcgcgg
      601 cggtgttcgg gcagcacgcc gtattgtttg agggcttcca gatgctgctt cgtgccataa
      661 cctttgtgtt tgtcgaaacc gtattgggga tggcgttgcg ccagtgcgta catttctgca
      721 tcgcgtgcgg tctttgccaa aacggatgcg gcggagattt cgatgatttt gctgtcgcct
      781 ttgacgacgg cttcggcagg gatacctaaa tgttctggaa tgcggttgcc gtcgatgaat
      841 attttttcgg ggtgcacaac caaaccgtca acggcgcgtt tcatggcaag catggtggcg
      901 tgcaggatgt tgaggctggc gatttcttcg ggcgtggcgg cggcgacgtg ccacgcggcc
      961 gcctgatctt taatcatttc ggcaagcgtg tcgcgttttt tctcgctgag ttttttggag
     1021 tcggtcagtc cgggtaggtc aaacgtttcc ggaaggatga cggcggcggc aaacacgctg
     1081 ccgactaaag gtccgcgtcc ggcctcgtcc acgccggcgg tcagtatgtg catgatgttt
     1141 cctgtcggga tggtacgatg ccgtctgaaa agggtttcag acggcatcgt accgatgtgt
     1201 ttatttcgcg tctttaaacc cgcgcttcaa atgcaccatc agcagtgcga cggcggcagg
     1261 cgttacgccg gaaatgcggc ttgcctgccc gacggtttcg ggtttgtgct ggttgagctt
     1321 ttgctgcact tctgccgaca agcctttgac tttgccgtaa tcgatgccgt cgggcagttt
     1381 taaggtttcg atgtcgcggc ggctgtcgat ttcttcgttt tggcggtcga tatagccttg
     1441 gtatttgact tggatttcga cttgttcgat gacttcggcg gagaggtttt cagacggcat
     1501 cgcgccttcg agcgtcatca gcgcggcgta gtcgaggttc gggcggcgta acaaatcgtg
     1561 caggttggct tctcggctga gtttttgtcc gaatacgcgg atttgttcgt cttcggcgag
     1621 tttttgcggt gtgtaccacg ttgttttcaa acgttggatt tcgcgttcga cggcttcgcg
     1681 tttttcgttg aacatacgcc attgcgcttc acccaccaag ccgattttgt agccgtcttc
     1741 ggtcaggcgc atgtcggcgt tgtcttccct gagttgcagg cggtattcgg cgcggctggt
     1801 gaacattcgg tagggttcgt tcacgccttt ggtgatgagg tcgtccacca atacgccgag
     1861 gtaggcttgt tcgcggcgca gcaggagcgg gtcttgtccg cgcacatatt gcacggcgtt
     1921 cgcgcctgcc aataaacctt gcgcggcggc ttcttcgtag ccggtcgtac cgttgatttg
     1981 cccagcgaaa aacagtccct caatggtttt ggtttcgagg ctggctttga ggttgcgcgg
     2041 gtcgaagtag tcgtattcga tggcgtagcc ggggcgcagg atatgggcgt tttccaaacc
     2101 tttcatactg cggacgagcg cgatttggat gtcgaacggc aggctggtgg agataccgtt
     2161 aggatagtat tcgtacgtag tcaggccttc gggttctagg aaaatctggt ggctgtcttt
     2221 atcggcgaaa cggttgattt tgtcttcgat agacggacaa taacgcgggc ccacgccttc
     2281 gattttgccg gtaaacatcg ggctgcggtc gaagcctgag cggatgatgt cgtgggtttg
     2341 cgtgttggta tgcgtaatcc agcaggacac ttggcgcggg tgcatttcgg cgttgccgcg
     2401 cacggacatg acgggaacgg gtgtgtcgcc gggctgttcg gtcagttggg aaaagtcaat
     2461 cgtgcgtccg tcgatgcgcg gcggcgtgcc ggttttcaga cggccttgcg gcagcttcaa
     2521 ttcgcgcaaa cgtccgccca gcgatttggc ggcggggtcg ccggcgcgtc cgccctcgta
     2581 gttttccaaa ccgatgtgga ttttgccgga caaaaacgtg cctgcggtca acaccacggc
     2641 gcgtgcttta aactccacgc ccatcgcggt gattacgcct gaaatgcggt cgccatcgag
     2701 cgttacgtct tcgacggctt gttggaaaag gtcgaggttt tcttggtttt ccaacatttc
     2761 gcggatggcg gctttatata ggatacgatc cgcctgcgcg cgcgtggccc gtactgccgc
     2821 gcctttgctg gcgttcagac ggcggaactg gatgccggat ttgtcggttg ccaacgccat
     2881 cgcgccgccg agcgcgtcga gttcgcgcac caaatgccct ttgccgatgc cgccgatgga
     2941 agggttgcac gacatttgtc cgagcgtttc aatattgtgt gtgagcaaaa gcgtctgcgc
     3001 gcccatacgg gcggcggcga gcgcggcttc tgtgccggcg tgtccgccgc cgacgacgat
     3061 aacgtcgtag gttttggggt aaatcatgtg ggtcatgtgt gtattgcctg tcggtgtttc
     3121 agacg
//
LOCUS       DAWFAN010000183         3086 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWFAN010000183 DAWFAN010000000
VERSION     DAWFAN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.183"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(297..1286)
                     /gene="pdxA"
                     /locus_tag="WGO65_001847"
     CDS             complement(297..1286)
                     /gene="pdxA"
                     /locus_tag="WGO65_001847"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ0328097.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAAEAVEAGRLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFCIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRRERINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1446..2873
                     /locus_tag="WGO65_001848"
     CDS             1446..2873
                     /locus_tag="WGO65_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0328098.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT          633 a          846 c          868 g          739 t
ORIGIN      
        1 cgcccaaagg cctggattcc cgcctgcgcg ggaatgacgg tttagaagtt gcccgaaacc
       61 tcaaaaaaaa ccgaaaccga acaagccgga ttcccgcaac ctttcgtcat tcccgtgaaa
      121 acgggaatct agaacctcaa actttcagat aatctttgaa tattgccgtc gcccgaaggt
      181 ctggattccc gcctgcgcgg gaatgacgat ttggaaatta cccgaaaccc aaaaacaact
      241 gaaaccgaac agaccggatt cccgcctgcg cggaaatgac gggtctttta tcatctttaa
      301 aggctgccgc gcgccatctc gacggcggtc tccacggcag ttatcaggct gccggaatcc
      361 gccctgccgg ttgccgccaa atcgagcgcg gtgccgtgat cgacggaggt gcggataaag
      421 ggcagcccca gtgtgatgtt cacgccctgc ccgaaactgt ggtatttcaa cacgggcagc
      481 ccttggtcgt ggtacatcgc caatacggca tccgcacctt cgagcataaa cggttggaac
      541 aatgtgtccg ccggatacgg gccggcgagg tttatccttt cgcggcgcag gttttcgagc
      601 gcggggataa tggtgtcggt ttcttcgtgt ccgaggtgtc cgccctcacc ggcgtggggg
      661 ttgagtccgg cgaccaggat tttgggattt ttgatgcaga atttgtgttt taagtcgtga
      721 tgcaaaatgc gtgcgacgct ttcaatcagc ggttgcgtga tggcggcggc aacgtctttc
      781 agcggcaggt gggtcgttac gagggcgacg cgcaggcctt tgccggcaag catcatcacg
      841 acctgccccg tgccgctttt ttccgccaga tattcggtgt gtccgctgaa aaaacctgtg
      901 cttgcgcgcg cgtcgttgat gatgcctttg tgcagcggcg cggtaacgat gccgtcgaaa
      961 atgccgtctg aaatgcctgc gagcgcggtg tccaaaagtt gcagcacata ggcggcgttg
     1021 gcgggattga gccgccctgc ctcaaccgct tcggcggcag ggatgtgcag cacttccagc
     1081 tcgccgtatg ccgcgccgcc tgattccgga tcgaagtcgc gcaagacgac gcttttgccc
     1141 aaggcttcgg cgcgcgcgcg caataggttt ttgtcgccca ataccgcgca gcggcagggc
     1201 aggcgtgcaa acgccaagtc caaacaaata tcggggccga tgccggcagg ctcgccggaa
     1261 gtaacggcaa aaacaggctg tttcatcgtg tttgctccaa acaaaatgcg attctaacgc
     1321 cgcagccgcg cggcgatgta aatttttctg attttgttga caatctgcta gaatgggcgt
     1381 ttacaaaatt taaaccctgc ttgcataccg ccaatatgtg cgagtttcaa ctttaaggaa
     1441 gcgatatgaa cgagaacttt accgaatggc tgcacggctg ggtcggcgcc atcaacgatc
     1501 cgatgtggtc atacttggtt tatatgcttt tgggtacggg gcttttcttc accgtaacca
     1561 cgggctttgt ccaattccgc ctgttcgggc gcagcatcaa agaaatgctc ggcggccgca
     1621 aacaggggga cgaccctcac ggcatcacgc cgtttcaggc atttgtaacc ggccttgcca
     1681 gccgcgtggg cgtgggcaat atcgcgggcg tggccatcgc catcaaagtc ggcggaccgg
     1741 gcgcggtgtt ttggatgtgg gtaaccgcct taatcggtat gagttcggcg tttgtcgaat
     1801 cttcgctggc gcagctcttt aaagtccgcg actacgacaa ccaccatttc cggggcggcc
     1861 ctgcctacta catcactcaa gggctggggc agaaatggct gggcgtgttg ttcgccctga
     1921 gcctgatttt ctgtttcggc tttgtgtttg aagcggttca gaccaatacc attgccgata
     1981 ccgtcaaagc ggcgtggggt tgggagcctc attatgtcgg cgtcgccttg gtgattttaa
     2041 ccgcgccgat tatcttcggc ggcatcaggc gcatatctaa agcggcggaa atcgtcgtcc
     2101 ccctgatggc ggttttgtac ctctttatcg cgcttttcat cattttgacc aatattccga
     2161 tgattccgga cgtgttcggt cagatttttt cgggcgcgtt caaattcgac gcggcagcag
     2221 gcggcttact cggcggtctg atttcgcaaa cgatgatgat gggcatcaaa cgcggcctgt
     2281 attccaacga ggcgggtatg ggttccgcgc cgaacgccgc cgccgccgcc gaagtgaaac
     2341 accctgtttc gcaaggtatg attcaaatgc tgggcgtgtt tgtcgatacc atcatcgttt
     2401 gttcttgcac cgccttcatc atcttgattt accaacagcc ttacggcgat ttgagcggtg
     2461 cggcgttgac gcaggcggcg attgtcagcc aagtggggca atggggcgcg ggcttcctcg
     2521 ccgtcatcct gtttatgttt gccttttcca ccgttatcgg caactatgcc tatgccgagt
     2581 ccaacgtcca attcatcaaa agccattggc tgattaccgc cgttttccgt atgctggttt
     2641 tggcgtgggt ctatttcggc gcggttgcca atgtgccttt ggtctgggat atggcggata
     2701 tggcgatggg cattatggcg tggatcaacc ttgtcgccat cctgctgctc tcgcccttgg
     2761 cgtttatgct gctgcgcgat tacaccgcca agctgaaaat gggcaaagac cccgagttca
     2821 aactttccga acatccgggc ctgaaacgcc gtatcaaatc cgacgtttgg taaatcccgc
     2881 ccttaccgga gccgcttccc cgcgaagcgg cttttccctt tccgcacact gtaaaaacag
     2941 ggcgaacaag cgtacaatcc caacccttta cttttgaatc catttcgttt ttcagacggc
     3001 atattgaata tagtggttta acaaaaatca ggacaaggcg acgaagccgc agacagtaca
     3061 agcagtacga aaccgattca cttggt
//
LOCUS       DAWFAN010000184         3083 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWFAN010000184 DAWFAN010000000
VERSION     DAWFAN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.184"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            110..2878
                     /locus_tag="WGO65_001849"
     CDS             110..2878
                     /locus_tag="WGO65_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0328099.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGGLKFNSNSGKWER
                     DFQKSYWKTKWYQKYDDPQELQKYIEGHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYREHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
     gene            2908..>3083
                     /locus_tag="WGO65_001850"
     CDS             2908..>3083
                     /locus_tag="WGO65_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328100.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVVY"
BASE COUNT          863 a          768 c          753 g          699 t
ORIGIN      
        1 attgaaatct tcagacggta tatcgaattt acacttttta atgtttatgc cgcctaaaaa
       61 aatgctaata tatttcttaa ttgtctgact gtttattgtt gaggaaaata tgagatcttc
      121 tttccggttg aagccgattt gtttttatct tatgggtgtt atgctatatc atcatagtta
      181 tgccgaagat gcagggcgcg cgggcagcga ggcgcagata caggttttgg aagatgtgca
      241 cgtcaaggcg aagcgcgtac cgaaagacaa aaaagtgttt accgatgcgc gtgccgtatc
      301 gacccgtcag gatatattca aatccagcga aaacctcgac aacatcgtac gcagcatccc
      361 cggtgcgttt acacagcaag ataaaagctc gggcattgtg tctttgaata ttcgcggcga
      421 cagcgggttc gggcgggtca atacgatggt ggacggcatc acgcagacct tttattcgac
      481 ttctaccgat gcgggcaggg caggcggttc atctcaattc ggtgcatctg tcgacagcaa
      541 ttttattgcc ggactggatg tcgtcaaagg cagcttcagc ggctcggcag gcatcaacag
      601 ccttgccggt tcggcgaatc tgcggacttt aggcgtggat gacgtcgttc agggcaataa
      661 tacctacggc ctgctgctaa aaggtctgac cggcaccaat tcaaccaaag gtaatgcgat
      721 ggcggcgata ggtgcgcgca aatggctgga aagcggagca tctgtcggtg tgctttacgg
      781 gcacagcagg cgcagcgtgg cgcaaaatta ccgcgtgggc ggcggcgggc agcacatcgg
      841 aaattttggc gcggaatatc tggaacggcg caagcagcga tattttgtac aagaaggcgg
      901 gttgaaattc aattccaaca gcggaaaatg ggagcgggat ttccaaaagt cgtactggaa
      961 aaccaagtgg tatcaaaaat acgatgaccc ccaagaactg caaaaataca tcgaaggtca
     1021 tgacaaaagc tggcgggaaa acctggcgcc gcaatacgac atcaccccca tcgatccgtc
     1081 cagcctgaag cagcagtcgg caggtaatct gtttaaattg gaatacgacg gcgtattcaa
     1141 taaatacacg gcgcaatttc gcgatttaaa caccaaaatc ggcagccgca aaatcatcaa
     1201 ccgcaattat caattcaatt acggtttgtc tttgaacccg tataccaacc tcaatctgac
     1261 cgcagcctac aattcgggca ggcagaaata tccgaaaggg tcgaagttta caggctgggg
     1321 gcttttaaag gattttgaaa cctacaacaa cgcaaaaatc ctcgacctca acaacaccgc
     1381 caccttccga ctgccccgtg aaaccgagtt gcaaaccact ttgggcttca attatttcca
     1441 caacgaatac ggcaaaaacc gctttcctga agaattgggg ctgtttttcg acggtccgga
     1501 tcaggacaac gggctttatt cctatttggg gcggtttaag ggcgataaag ggctgctgcc
     1561 ccaaaaatca accatcgtcc aaccggccgg cagccaatat ttcaacacgt tctacttcga
     1621 tgccgcgctc aaaaaagaca tttaccgctt aaactacagc accaataccg tcggctaccg
     1681 tttcggcggc gaatatacgg gctattacgg ctcggatgac gaatttaagc gggcattcgg
     1741 agaaaactcg ccgacataca gggaacattg caacccgagt tgcgggcttt atgaacccgt
     1801 attgaaaaaa tacggcaaaa agcgcgccaa caaccattcg gtcagcatta gtgcggactt
     1861 cggcgattat ttcatgccgt tcgccagcta ttcgcgcaca caccgtatgc ccaacatcca
     1921 agaaatgtat ttttcccaaa tcggcgactc cggcgttcac accgccttaa aaccagagcg
     1981 cgcaaacact tggcaatttg gcttcaatac ctataaaaaa ggactgttaa aacaagatga
     2041 tacattagga ttaaaactgg tcggctaccg cagccgcatc gacaactaca tccacaacgt
     2101 ttacgggaaa tggtgggatt tgaacgggga tattccgagc tgggtcagca gcaccgggct
     2161 tgcctacacc atccaacacc gcaatttcaa agacaaagtg cacaaacacg gttttgagtt
     2221 ggagctgaat tacgattatg ggcgtttttt caccaacctt tcttacgcct atcaaaaaag
     2281 cacgcaaccg accaacttca gcgatgcgag cgaatcgccc aacaatgcgt ccaaagaaga
     2341 ccaactcaaa caaggttatg ggttgagcag ggtttccgcc ctgccgcgag attacggacg
     2401 tttggaagtc ggtacgcgct ggttgggcaa caaactgact ttgggcggcg cgatgcgcta
     2461 tttcggcaag agcatccgcg cgacggcaga agaacgctat atcgacggca ccaacggggg
     2521 aaataccagc aatgtccggc aactgggcaa gcgttccatc aaacaaaccg aaactcttgc
     2581 ccgccagcct ttgatttttg atttttacgc cgcttacgag ccgaagaaaa accttatttt
     2641 ccgcgccgaa gtcaaaaatc tgttcgacag gcgttatatc gatccgctcg atgcgggcaa
     2701 tgatgcggca acgcagcgtt attacagctc gttcgacccg aaagacaagg acgaagacgt
     2761 aacgtgtaat gctgataaaa cgttgtgcaa cggcaaatac ggcggcacaa gcaaaagcgt
     2821 attgaccaat tttgcacgcg gacgcacctt tttgatgacg atgagctaca agttttaaag
     2881 gcagcccgca ttttgtagaa aaccgcaatg ccgtctgaaa gcccttcaga cggcatttgt
     2941 ttccccaaac gcatcatcct gccgcaagcc tatgccaatc cgttttatcg catcggcaac
     3001 tcaaagaaaa atccatttca ttcccacgca gggaagccgg tttttgattt cggttatttt
     3061 tggttgtttc gggtagttta tga
//
LOCUS       DAWFAN010000185         3072 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWFAN010000185 DAWFAN010000000
VERSION     DAWFAN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.185"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(92..943)
                     /locus_tag="WGO65_001851"
     CDS             complement(92..943)
                     /locus_tag="WGO65_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ0328101.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1286..2800
                     /gene="katA"
                     /locus_tag="WGO65_001852"
     CDS             1286..2800
                     /gene="katA"
                     /locus_tag="WGO65_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0328102.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
BASE COUNT          741 a          868 c          791 g          672 t
ORIGIN      
        1 gatgcaggtt tccgcgcgga cggattcggc attcccgctt tcgcagaaat gccggctttt
       61 ggtttgttgt ctttataaga ttattcggca attattctgc ggttttgcgt ttgtgtgctg
      121 ccggaatctc aaaggattct ctgtatttgg caatggtgcg gcgagaaacc tccatgccgc
      181 ggaaagccag caggttggcg agcgcctcgt cagaatacgg cgtgggtgtg gacgaggcaa
      241 aaccagcggc gggaaaggga ttgagcgaag caatgaggtc gagtgcggct tcgagtgtgc
      301 cgctgtcggt ttggggcagg cgttttttta ttcgcgcgag ggtttggctg cggttgccgt
      361 caatgctgtc gagggcgttt cggacgatat gcagggcgga gggtttggca gcacattcgc
      421 ccgatctttc tatctgcagt atcagcgatt cgttcaaatc ggcggcggcc acgcctgccg
      481 ggtcgaattt tttcaatgcg gtcagggctt gtttcagcat tgcttcatcc aacatccact
      541 ccaagggcgt atggtcgagg atgtcttcga tgctgtcggt cagataacct tgctcgtcaa
      601 ggaaatcgat gaggatgtgg acacaggcgg attcttgatc ggaaagcggg tgttcgcata
      661 cttgcgcgtg caggtattgc ttgaaatcct gctcgccggc gatgttggac agcatatctt
      721 cgccttcgtc tccgccgatt tgacgggcag gcgcagtgta atggctgaac tcggcatcgg
      781 aaaattcatc cgtatctttg cgttcgagca gggggttgtc cgacagccag ttttcgacct
      841 cgcgttcaag ttcgataccc gacatctgca atacgcgcaa agattgttgc agccgctggt
      901 tgagctgctg ggtctgtttg agctttattc cgagtaacgt catgataatg cgaaagaatt
      961 attattttgg cctgtcggtg gcagatgatg ccgggaaaca ttattataaa tggttttatg
     1021 tagggatttt gattcgtgcc gataacaaaa aacaaacgaa aatcaaaaac cgattgctta
     1081 taacaatatt aaatcgattt catagtttta atagcgaaaa tcttagcgta tagtcgcatc
     1141 catagttttt acaaaaggaa aacaaaatgt cgattcaaga aatttattgc aatcaagaaa
     1201 ccggttacga atacgctttc cgccaaatcg tactgtaagc cgtttgtcgt atcttttgat
     1261 taaccaagga aaaggaacac gagctatgac tacctccaaa tgccccgtaa cccatctgac
     1321 catgaacaac ggcgcgcctg ttgccgacaa tcaaaacagc ctgaccgccg gtccgcgcgg
     1381 ccctctgctg gcgcaggatt tgtggctgaa tgaaaaactc gccgacttcg tgcgcgaagt
     1441 catccccgaa cgccgtatgc acgccaaagg ttcgggcgcg ttcggtacgt ttaccgtaac
     1501 gcacgacatc accaaataca cccgcgccaa aatcttcagc gaagtcggca aaaaaaccga
     1561 gatgttcgcc cgtttcacca ccgtggcagg cgaacgcggc gcagccgatg cagaacgcga
     1621 catccgcggt tttgccttga aattttatac cgaagaaggc aactgggatg tggtcggcaa
     1681 caacacgccc gtgttcttcc tgcgcgaccc gcgtaagttc cccgacctga acaaagccgt
     1741 caaacgcgac ccgcgcacca atatgcgctc tgccacaaac aactgggact tctggacgct
     1801 gctgcccgaa gcactgcacc aagttaccat cgtgatgagc gaccgcggca tccccgccgg
     1861 ctaccgccat atgcacggct tcggttcgca tacctacagc ttctggaacg aagcaggcga
     1921 gcgtttttgg gtgaaattcc atttccgcac ccaacaaggc attaaaaacc tgaccaacga
     1981 agaagccgcc aaaatcatcg ccgacgaccg cgaaagccat cagcgcgact tatacgaagc
     2041 catcgaacgc ggcgagtttc cgaaatggac gatgtacatc caagtcatgc ctgaagcaga
     2101 cgcggaaaaa gtaccttatc atccgtttga cttgaccaaa gtttggccga aaaaagacta
     2161 tccgctgatt gaagtgggcg aattcgagtt gaaccgcaat cccgaaaact tcttcgccga
     2221 tgtggaacaa tccgccttcg caccgagcaa cctcgttccc ggtgtcggcg ccagcccaga
     2281 taaaatgttg caagcccgtt tgttcaatta cgccgacgcg caacgctacc gcttgggcgt
     2341 gaacttccgc caaatccctg tcaaccgtcc gcgttgccct gttcacagca accagcgcga
     2401 cggacaagga cgcgccgacg gcaactacgg cagcctgccg cactacgaac ccaacagctt
     2461 cggccaatgg cagcagcagc ccgacttcgc cgaaccgcct ttgaaaatca acggcgacgc
     2521 ggcacactgg gactaccgcc aagacgatga cgactatttc agccaaccgc gcgccctgtt
     2581 caacctgatg aacgacgcgc agaaacaggc attgttcggc aacaccgccg ccgcaatggg
     2641 cgacgcgccc gacttcatca aataccgcca tatccgcaac tgctaccgtt gcgatccggc
     2701 atacggcgaa ggcgtggcaa aagccctcgg actgactgtc gaagacgcac aagccgcccg
     2761 cgcgaccgac cccgcactcg gccagcccgg tttgctgtaa gggggcatta tgtggatgga
     2821 aattgaagaa atcctgtccc gcgccgtccg ctaccggaaa taaccgggca taaaaatgcc
     2881 gtctgaaacg tcgtttgacc gcttcagacg gcatttcccc atcccgcccc cgccgtttca
     2941 gtgggcgttt tttattaaac gcaaaatatc ccgtcattcc cgccccacag cccacttttc
     3001 cgccgtcatt cccgcaccat ccccaccggt tcaaaccggc acgaaaactt ttccgcgtca
     3061 ttcccgcgaa ag
//
LOCUS       DAWFAN010000186         2959 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWFAN010000186 DAWFAN010000000
VERSION     DAWFAN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.186"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(137..1879)
                     /gene="hrpB"
                     /locus_tag="WGO65_001853"
     CDS             complement(137..1879)
                     /gene="hrpB"
                     /locus_tag="WGO65_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ0328103.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISTFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITADLDAAAPFILGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            complement(1940..2179)
                     /locus_tag="WGO65_001854"
     CDS             complement(1940..2179)
                     /locus_tag="WGO65_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0328104.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTDL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            complement(2463..2765)
                     /locus_tag="WGO65_001855"
     CDS             complement(2463..2765)
                     /locus_tag="WGO65_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328105.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
BASE COUNT          825 a          660 c          629 g          845 t
ORIGIN      
        1 gataatgcga tgtaaacctt tattcatgtc tattccattt tgaagatgaa cgtactgcgc
       61 gccaagtacg taggtaaagt ttgacggtct gaggataagg aaagaccgtc taaatatcag
      121 taaaaaaatt cagaggttag aaactgtaat tcaagttgaa gccgtaaacg gtgttggtcg
      181 tctgaaagcc tttgggttta tgaagcggct tgccggcaaa cagatcataa gcaaacatac
      241 cgcctacttt atgccctcct ctgaagccga ccactgcacc catcagctgc ttgcccgata
      301 catattgtgc actttcgcca gatacgcggc catagtccgc accgagatag aactgatggt
      361 tcggatgaaa ataccaagtt aaagtattct gccagtagaa acctcgctct ccgaaaagac
      421 tctgctcccc atcaaatccg cgaacggtgt agcggctgcc gattgacaat ttatcttggg
      481 caaccaacgg cgttttgttc cattgagctt gaatggcggt tgcgtagaaa aactgctgtt
      541 tgcctaaaat aaatggggcg gctgcgtcca aatcggcagt aatgattttc atacgagatg
      601 tacctggaag aatatcgccg ccgttttctt caggtgcggg catactttgg cgcataccgg
      661 tcccgcgttt gtaagacaac ttgccgtcaa gctgccaacg gttgaggtaa gcacggtggc
      721 gcaattcggc ttcccagcct gcagagcggc ggcgttgcac ttcgatttcg gcatcgtcga
      781 tgtatttata ggtttggcgt gtccataatt tcattccgac tgaagtttta tgaaacctgt
      841 tacgccaaag catgcgctcg gcggccaggc tgctctgata ttgtttgccg ttgtaatcgt
      901 aattgacgga atagccttcg gttgcttcgt ggtaacgatg tccattgtga ttaaaagaaa
      961 acagccattt ttttacgggc accgaataat gcacgctgta acttctggat ccgctttcag
     1021 tttccgtacc ggtggcatca gtcaagtccg ttttgtgcgc caaaccgcgt ccatatgaaa
     1081 cataaaacaa atcgcttaag cccaaagggt tatcgaacga taaagcgaca tttccttgat
     1141 atttgccggt cgttttgccg cccgcatcat ctataccgat actgaaccgt atgggtttat
     1201 tctgctgcca tttgatctgt aaatcgcttt tgccttcttc ttcggacggt ataatctgaa
     1261 tatctgtttt aacactcgac aaacgacgca ggttttccaa gccctgctct acatcgcgaa
     1321 gattgagaat tttgttccta tataagggaa atttgttatt gaatgtacta atactgcctt
     1381 cggcagactt cccatcccgt ttttcttcat agcggatatc ccctatttcg cctgctgata
     1441 cccgtaattt cagaattccc gaatccatat tctgtggttg gataatagct tgggaagtga
     1501 ggtagccacg cacaatcagt atctgttgcg cggctttttg tagcctgctc aaattattgg
     1561 aacctaaaca catcccagtt ttaaaagctg tttctttcat gagcacagaa ggaagaaaag
     1621 aaaatttgcg cgccgtctta tcatctaaac taatgtaatt tacccgagta cacggtgttt
     1681 catcttcact caggacataa ttgttcttct ccaatggttg ctcgaaacgg acatttgcat
     1741 cagttaacaa ttcagcatct atgtgctgct gacgctgcat ggaacggata agttctgcat
     1801 cgttttcatc ggcagctaag gttttaaggg gtatgacagc caggataacc aacagacatg
     1861 gagcaggaaa aaatttcatg acatcaatat tattttagca atatttacta ttttgtcata
     1921 aatttaaaag tatttacagt tatagaatga gacctttgca aaattcccca aaattcccac
     1981 caagacattt aggggatttt ggggaatttt gcaaaggtct cggacagtat tttgaacgca
     2041 gtgcgcgtaa atccgtatgg aaaccatgaa atcccgccac agccgccaga catgccaagc
     2101 cgcattctga tatttctgtt tgcaggataa caggcagctt tttctttaag cccaaagaca
     2161 ggttttgcag atggggcata gatttccttt ttgaaaaata gggattagga agttggatgt
     2221 attttagaaa ggccgcctga aaaggtttca gacgaccttt tgcgactagc tgctatttta
     2281 tttaaagctt ttctctaaca aacgagctaa tatttttcct gtaataaaac agataaaaaa
     2341 cagcatccaa tacgtcagat tggaaaaatc ggtcgtatag agaatcaaca tataaagaag
     2401 cagcatgatg ccgagtgcga tgaattgata atgtttggca aacatcatga cctcctcaac
     2461 tattaaggca aaccgcctga atattctcgt tcaatcgttt cggcaatttc cctataacgt
     2521 cgataccatg accagtcgaa attttcaatg gcatggctcg caaacgtacc aaattcaggc
     2581 atccctatgc ggctacctgc taaagctccg attgtagctc cccaaccagg cggattactg
     2641 aacgtattgt ttggcaatcc taaagattta tcaggatttc ccgtatcgtt aagccctgca
     2701 gattcgcaaa tttcgcaata atatgagctt tgttgcgcat tacctgagcc tccgacccaa
     2761 gtcatttcat gaacacatat agtggattaa atttaaatca ggacaaggcg acgaagccgc
     2821 agacagtaca aatggtacgg caaggcgagg caacgctgta ctggtttttg ttaatccact
     2881 ataaaactct cattttgaaa ctccttgtat cgttaatcaa acaatcaaaa gggcagatgc
     2941 cctatccttg cttttacag
//
LOCUS       DAWFAN010000187         2935 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWFAN010000187 DAWFAN010000000
VERSION     DAWFAN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.187"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            162..1274
                     /gene="nadA"
                     /locus_tag="WGO65_001856"
     CDS             162..1274
                     /gene="nadA"
                     /locus_tag="WGO65_001856"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ0328106.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNSGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            1326..2834
                     /gene="nadB"
                     /locus_tag="WGO65_001857"
     CDS             1326..2834
                     /gene="nadB"
                     /locus_tag="WGO65_001857"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ0328107.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLSQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRCPNIRVYERQMALDIQTES
                     GAAYGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDLRAELAPR
                     DIVARDIAAEIAKQTQDFVSLDISHQPAAFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQTVPSQRSSEHPSAEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLHR
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT          685 a          903 c          807 g          540 t
ORIGIN      
        1 caaagcggtt tgtcggaacg aagcgggtcg gttttacggt atcgcaaacc tgttttcaga
       61 cgaccttgct tattatactc aagttgagca taatataaaa cagtttgaaa agcagacggc
      121 aaaccttcat gccgtctgaa aaccagctac aaggaaacat catgcaaacc gccgcccgcc
      181 gctcgttcga ctacgatatg cccctcatcc aaacgccgac ttccgcctgc caaatccgtc
      241 aggcgtgggc gaaggttgcc gatacgcccg accacgagac ggcaggccgt ctgaaagacg
      301 aaatcaaggc tttgctgaag gagaaaaacg cggtcttggt ggcgcattat tacgtcgatc
      361 cgctgattca ggatttggct ttggaaacgg gcggatgcgt gggcgattcg ctggaaatgg
      421 cgcgcttcgg tgcggaacac gaagccggta cgctggtggt ggctggtgtg cgcttcatgg
      481 gcgagagcgc gaaaatcctc tgccctgaaa aaacggtgct gatgcctgat ttggaggcgg
      541 aatgttcttt ggatttgggt tgtcccgaag aagcgttttc agcgttttgc gaccaacacc
      601 ccgaccgcac ggtggtggtg tacgccaaca cttccgccgc cgtgaaagcg cgtgccgact
      661 gggtggtaac gtcttcggtg gcgttggaaa tcgtgtcgta tttgaaatcg cgcggcgaga
      721 aactgatttg gggacccgac cgccacctcg gcgactacat ccgccgcgaa acgggcgcgg
      781 atatgctgtt gtggcagggt tcgtgcatcg tccataacga attcaaaggg caagagctgg
      841 cggcgttgaa ggcggaacat cccgacgcgg tggtgctggt tcatcccgaa tcgccgcaaa
      901 gcgtcatcga actgggcgac gtggtcggct cgaccagcaa actgctcaaa gccgccgtat
      961 cgcgtcctga aaaaaaattc atcgtggcga ccgatttagg catcctgcac gaaatgcaaa
     1021 agcaggcgcc cgacaaagaa tttatcgccg caccgacggc gggcaacagc ggaagctgca
     1081 aaagctgcgc gttctgcccg tggatggcga tgaattcgct gggcggcatc aaatacgccc
     1141 tgacaagcgg gcacaacgaa atcctgttgg acagaaagct gggcgaagcc gccaaactgc
     1201 ctttacagcg tatgctcgac ttcgcggcag gactcaaacg cggggatgtg ttcaacggca
     1261 tgggccccgc ctgatttgcc gtcaatattg ccgtttcaga cgacctctca aacaaggaca
     1321 acaccatgca aaccgattgc gacgtattga ttgccggaaa cgggctggcg gcactgacgc
     1381 tcgccctgtc gctgcctgaa tcgttccgca tcgtcatttt gtgcaaaaac cggctggacg
     1441 acaccgccag ccgtcatgcg caaggcggga ttgcggcggc gtggtcggga gaggacgaca
     1501 tcgaaaaaca cgttgccgat accttggaag ctggcgcggg tttgtgcgac gaagccgccg
     1561 tccgcaccat cctgtcgcag ggcaaaccgg caatcgaatg gctgctgtcg cagggcgtgg
     1621 cgttcgacca aaataataac ggcctgcacc tgacacgcga aggcgggcat acctgccgcc
     1681 gaatcgccca cgtcgccgat tacacgggcg aagccgtcat gcagagcctg attgcccaaa
     1741 tacgccgctg cccgaacatc cgcgtttacg agcggcagat ggcgttggac atccaaaccg
     1801 aatcaggcgc agcgtacgga ctgaccgtcc tcgactgccg aacgcaagaa acctaccgca
     1861 tccgcgcccg ccataccgta ctcgcaggcg gcggcttggg acagatttac gccgccacca
     1921 ccacgccgcc cgaatgcacg ggcgacgcca tcgccatggc aatacgcgca ggctgcgcag
     1981 tcgaaaacct cgaatttatc caattccacc ccacaggctt ggcaaggccg tctgaaaacg
     2041 gacgcacctt cctgatttcc gaagccgtgc gcggcgaagg cggcattctg accaaccaat
     2101 cgggcgaacg gtttatgccg cattacgacc tccgtgccga actcgcaccg cgcgacatcg
     2161 ttgcccgcga catcgccgcc gaaattgcca aacaaacgca agacttcgtc tcgctcgata
     2221 tcagccatca acccgcagcg ttcgtccgtc agcatttccc gtccatccat cggcactgcc
     2281 tgtcccaatg cggcttggac atcacgcgcc aagccatccc cgtccgcccc gtgcaacact
     2341 acacctgcgg cggcatccaa accgaccctt gcggcagaac ctccctgccg cagctctacg
     2401 ccttaggcga aaccgcctgc acagggctgc acggagccaa ccgcctcgcc agcaattccc
     2461 tgctcgaatg cgtcgttacc gccagacttg ccgcccaaac catcgcggac ggacaagcat
     2521 tccaaaccgt accgtcccaa aggtcgtctg aacacccctc cgccgaagca ggcatctttt
     2581 cagacggcat ccaaagcgca ttcagccgcc ccgtcctgca agccttcaac caacgccatc
     2641 tgggcatcct ccgcaacgat accggcctgc accgcgccat cgcccaactg cggctttgga
     2701 agcaaaacca aaccgaaccg cacaccgcgt ccgaatacga aaaccgcaac ctgctcgaat
     2761 gcagcctcgc cgtcgcccaa gccgcataca ggcggagaca gaacatcggc gcgcatttta
     2821 atagtgattg ttaaggttgg ctggttagaa aaaaatcgtt caaataaatt tgaatagcat
     2881 ggttgagaaa ttggcattca gtttttaggg ttgcgtaagt tgggttatca atctg
//
LOCUS       DAWFAN010000188         2867 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWFAN010000188 DAWFAN010000000
VERSION     DAWFAN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.188"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(81..557)
                     /gene="greA"
                     /locus_tag="WGO65_001858"
     CDS             complement(81..557)
                     /gene="greA"
                     /locus_tag="WGO65_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ0328108.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            952..2253
                     /gene="aroA"
                     /locus_tag="WGO65_001859"
     CDS             952..2253
                     /gene="aroA"
                     /locus_tag="WGO65_001859"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ0328109.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     ENYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPKTLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETTE"
     gene            complement(2296..2829)
                     /locus_tag="WGO65_001860"
     CDS             complement(2296..2829)
                     /locus_tag="WGO65_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0328110.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          604 a          791 c          771 g          701 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat aatttcctgc gtgtgtcggg
       61 tgtatcgaaa tcaagccgaa tcaaatatat cggacttcga taatgtcgta ttcgcgcacg
      121 ccgcccgggg cttggacttc cgccgtatcg ccctcttcct tgccaattaa ggcgcgggca
      181 atcggtgagc cgacatagat tttgccctgt ttgatatccg cttcgtcttc gccgacaatt
      241 tggtaggtaa cgtgttcttc cgtttccaaa tcttccagcg taaccgtcgt accgaacacg
      301 attttgcctt cggcgtggat ttcggtcgga ttgatgatgt gggcaacgga aagtttgtgt
      361 tccaactcgg aaatgcggcc ctcgataaaa ccttggcgtt ctttggcggc ttcatattcg
      421 gcgttttcgg acaaatcgcc gtgcgatctg gcttcagcaa tcgcttcgat cacttcgggg
      481 cgcgccacgc ttttaagctg ctgcaattcc tgtttcagca attccgcacc gcgtacggtc
      541 agggggattt tttgcatcgg tcttttttct ccatattccg gcacaccggt ttgcggcagc
      601 aaacataccg cataccgtct tgttttgtgc gtccggatat taaaataaaa acacaagccg
      661 cccggaaaat cggcggcttg tctgtcgttg aacagcggct attctaccaa attctatgaa
      721 attggcaatc gtaccgtacc gtccgcaaac gtcatctgtc cgcaacaaaa gccgaaaata
      781 tgccggcaaa aaaattttag aaacaaaaaa tttaaaaata atcaattttc ggcataaaaa
      841 accacattta cggactttaa aaccgaaaat gccaaacctg agatttttca tacagcattt
      901 gcaccagtat aatgcaggct gtttttatct ttataatatt gacgttttgc catgaccgaa
      961 tccctccgcc tccctgccgc ctcgctcaaa ccttccactg tcgccctgcc cggctccaaa
     1021 agcatcagca accgcaccct gctgcttgcc gccttgtccg acaatgcttg cgaaatccat
     1081 tccctgctca aatccgacga taccgaccgt atgctcgaag cactcgataa actcggcgtt
     1141 gaaatcgaat atcttgccga agaccgtctg aaagtgcacg gcacaggcgg acgcttcccc
     1201 aaccgcactg ccgatttgtt tttgggcaac gcgggcacgg cgttccgccc gctgaccgcc
     1261 gcgcttgccg ttttgggcgg cgattatcgc ctgcacggcg ttgcgcgtat gcacgaacgc
     1321 cccatcggcg atttggccga tgcactgcgg attgccggcg ccgatgtcga atatctcggc
     1381 aaggaacact atccgccgct tcatatcggc gaacgccaag acaacggcga gcgcgtgatt
     1441 ccgattaaag gcaatgtgtc cagccagttt ctgaccgccc ttttaatggc gttgccgctg
     1501 accgggcagg cgtttgaaat ccgtatggtc ggcgagttaa tttccaagcc ctatatcgac
     1561 attactttaa aactgatggc gcaattcggc gtacaggttg ccaatgaaaa ctaccgcgtc
     1621 ttcaaaattc ccgccgatgc gcactaccac gcgcccgaac acttgcacgt cgaaggcgat
     1681 gcctccagcg cgtcctactt cctcgcagcc ggtttgattg ctgccacgcc cgtgcgcgtg
     1741 accggcatcg gcgcaaacag catacagggc gatgtcgcct ttgcccgcga gctggaaaaa
     1801 atcggggcgg acgtggtttg gggcgaaaac ttcgtcgaag tttcacgccc gaaggaacgt
     1861 gccgtccaat cctttgattt ggatgcgaac catatccccg atgccgccat gaccctcgcc
     1921 atcgtcgcgc ttgctacagg gcaaacctgc acgctgcgca acatcggttc gtggcgcgtc
     1981 aaagaaaccg accgcatcgc cgcgatggca aacgagttgc gcaaactcgg ggcaaaagtc
     2041 gtcgaagaag ccgaagcaat tcacatcacc ccgcccaaaa cgctgacacc cgacgccgtc
     2101 atcgacacgt acgacgacca ccgcatggcg atgtgtttct cgctggtttc gctgttgggc
     2161 gtacccgtcg tcatcaacga tccgaaatgc acccataaaa ccttcccgac ttatttcgac
     2221 gtgttctctt cactgaccga aacaacggaa taaggcggca ttttgccgcg attccggcgc
     2281 ggcgcggcgg gcggctcatt ctgtaaagaa agtatgtgcg ccgaggtagt ttttggcgta
     2341 aaacggtgtg gagagttttt cggttttgat ggttttgccg ctgctggggg catggatgaa
     2401 ttcgccgttg ccgatataga gtccgacgtg tgagtagcgg tgtgtgccgc cggtgttgaa
     2461 gaatacgagg tcgccggcct taaggcggct gtcggggatt ttgcggcttg ccgccgccat
     2521 gtcgcgggcg gtgcgcggca gcttgacgtt gagggcgttt ttgtaaacga attgaatcat
     2581 gccgctgcaa tcgaagccgg ttgcggtgct gctgccgccc catttgtagg gcgtgccgat
     2641 gagtccgagg ctgtggagca tgagttcctg cgagccttgt gtgcggtcga tgtggctgat
     2701 gcggacggct tggatttgcc ggactgtctg tttgggtttc ggttggcggt gtttgccgga
     2761 ggtcgtgccg catgaggcga ggagcagtgc gctgagacag aggaaaaggg ttttgtcggg
     2821 gggaaacatg gtttttcctt tgcgggttcg gatatccgtc tgaaggt
//
LOCUS       DAWFAN010000189         2792 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWFAN010000189 DAWFAN010000000
VERSION     DAWFAN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.189"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            150..473
                     /locus_tag="WGO65_001861"
     CDS             150..473
                     /locus_tag="WGO65_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0328111.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            587..2764
                     /locus_tag="WGO65_001862"
     CDS             587..2764
                     /locus_tag="WGO65_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0328112.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          672 a          994 c          707 g          419 t
ORIGIN      
        1 cccctctccc tgtgggagag ggttagggag agggcggtaa gccgcaggct tacatcaaag
       61 ccgataacgc tttcgttaca actccgccca ctgaacgaag ccaaaacgac aaaccgcatc
      121 gtaaaccacc caacccatag gagaacccca tgcaaaacga aaccatcaac ctgaaacagc
      181 accttgccgc catcaaagaa tactggcagc ccgaaatcat caaccgccac gggttccaat
      241 tccgtttggt caaacttttg ggcgattacg gctggcatac gcacggatac agcgacaaag
      301 tgctgtttgc cgtggagggc gacatggcgg tggacttcgc cgacggcggc agcatgacga
      361 tacgcgaggg cgagatggcg gtcgtgccga agtcggtgtc gcaccgcccg cgttcggaaa
      421 acggctgctc gttggtgctg attgagttgt ccgacccgtc cgaggccgtc tgaaaacgaa
      481 gtttccgaag gaagctgagt ttctgcgaag ctaaaagcag cctgcacctt caatcaatat
      541 gccgaaaata caaccccacc gcacaccaac acacaaagga aatcccatga cacgcttcaa
      601 atattccctg ctgtttgccg ccctgttgcc cgtgtacgcg caggccgatg tttctgtttc
      661 agacgacccc aaaccgcagg aaagcactga attgccgacc atcaccgtta ccgccgaccg
      721 caccgcgagt tccaacgacg gctacactgt ttccggcacg cacaccccgc tcgggctgcc
      781 catgaccctg cgcgaaatcc cgcagagcgt cagcgtcatc acatcgcaac aaatgcgcga
      841 ccaaaacatc aaaacgctcg accgcgccct gttgcaggcg accggcacca gccgccagat
      901 ttacggctcc gaccgcgcgg gctacaacta cctgttcgcg cgcggcagcc gcatcgccaa
      961 ctaccaaatc aacggcatcc ccgttgccga cgcgctggcc gatacgggca atgccaacac
     1021 cgccgcctat gagcgcgtag aagtcgtgcg cggcgtggcg gggctgctgg acggcacggg
     1081 cgagccttcc gccaccgtca atctggtgcg caaacgaccg acccgcaagc cattgtttga
     1141 agtccgcgcc gaagcgggca accgcaaaca tttcgggctg ggcgcggacg tatcgggcag
     1201 cctgaacacc gaaggcacgc tgcgcggccg cctggtttcc accttcggac gcggcgactc
     1261 gtggcggcag cgcgaacgca gccgcgatgc cgaactctac ggcattttgg aatacgacat
     1321 cgcaccgcaa acccgcgtcc acgcaggcat ggactaccag caggcgaaag aaaccgccga
     1381 cgcgccgctc agctacgccg tgtacgacag ccaaggttat gccaccgcct tcggcccgaa
     1441 agacaacccc gccacaaatt gggcgaacag ccgccaccgt gcgctcaacc tgttcgccgg
     1501 catcgaacac cgcttcaacc aagactggaa actcaaagcc gaatacgact acacccgcag
     1561 ccgcttccgc cagccctacg gcgtagcagg cgtgctttcc atcgaccaca acaccgccgc
     1621 caccgacctg attcccggtt attggcacgc cgacccgcgc acccacagcg ccagcgtgtc
     1681 attgatcggc aaataccgcc tgttcggccg cgaacacgat ttaatcgcgg gtatcaacgg
     1741 ttacaaatac gccagcaaca aatacggcga acgcagcatc atccccaacg ccattcccaa
     1801 cgcctacgaa ttttcccgca cgggtgccta cccgcagcct gcatcgtttg cccaaaccat
     1861 cccgcaatac ggcaccaggc ggcaaatcgg cggctatctc gccacccgtt tccgcgccgc
     1921 cgacaacctt tcgctgatac tcggcggcag atacagccgt taccgcaccg gcagctacga
     1981 cagccgcaca caaggcatga cctatgtgtc cgccaaccgt ttcaccccct acacaggcat
     2041 cgtgttcgac ctgaccggca acctgtctct ttacggctcg tacagcagcc tgttcgtccc
     2101 gcaatcgcaa aaagacgaac acggcagcta cctgaaaccc gtaaccggca acaatctgga
     2161 agccggcatc aaaggcgaat ggcttgaagg ccgtctgaac gcatccgccg ccgtgtaccg
     2221 cgcccgtaaa aacaacctcg ccaccgcagc aggacgcgac ccgagcggca acacctacta
     2281 ccgcgccgcc aaccaagcca aaacccacgg ctgggaaatc gaagtcggcg gccgcatcac
     2341 gcccgaatgg cagatacagg caggttacag ccaaagcaaa acccgcgacc aagacggcag
     2401 ccgcctgaac cccgacagcg tacccgaacg cagcttcaaa ctcttcactg cctaccactt
     2461 tgcccccgaa gcccccagcg gctggaccat cggcgcaggc gtgcgctggc agagcgaaac
     2521 ccacaccgac cctgccacgc tccgcatccc caaccccgcc gccaaagccc gcgccgccga
     2581 caacagccgc caaaaagcct acgccgtcgc cgacatcatg gcgcgttacc gcttcaatcc
     2641 gcgcgccgaa ctgtcgctga acgtggacaa tctgttcaac aaacactacc gcacccagcc
     2701 cgaccgccac agctacggcg cactgcggac agtgaacgcg gcgtttacct atcggtttaa
     2761 ataaggtcgt ctgaaaacgg agtttctgcg aa
//
LOCUS       DAWFAN010000190         2737 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWFAN010000190 DAWFAN010000000
VERSION     DAWFAN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.190"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            32..511
                     /locus_tag="WGO65_001863"
     CDS             32..511
                     /locus_tag="WGO65_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ0328113.1"
                     /translation="MPSETAVADIARTYSAHYLNPNNRNMTLFPDALPCLDKLKAQGY
                     WLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGFDPKEA
                     LVVGDTAHDLHMAANAGAAAVGVATGAHSREQLRTAPHLAVLDGLSELPGFLAQHYA"
     gene            complement(598..1566)
                     /gene="ispH"
                     /locus_tag="WGO65_001864"
                     /gene_synonym="lytB"
     CDS             complement(598..1566)
                     /gene="ispH"
                     /locus_tag="WGO65_001864"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ0328114.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAEERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1595..2092)
                     /locus_tag="WGO65_001865"
     CDS             complement(1595..2092)
                     /locus_tag="WGO65_001865"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ0328115.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKH"
     gene            complement(2298..2402)
                     /locus_tag="WGO65_001866"
                     /pseudo
     CDS             complement(2298..2402)
                     /locus_tag="WGO65_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          625 a          838 c          658 g          616 t
ORIGIN      
        1 cctgcccgaa atcatccgcg acctgctcga aatgccgtct gaaaccgccg ttgccgacat
       61 cgcgcgcact tattccgcgc attacctcaa tcccaacaac cgcaatatga cgctctttcc
      121 cgatgccctg ccctgtctgg acaagctcaa agcacaagga tactggcttg ccgtcgccac
      181 gggcaaaggg cgggcgggct tggacaacgc catcagccaa accgccaccg gcggctattg
      241 gctcgccacc gcctgcgcgg gggaatatcc gtccaaaccc tcgcccgaaa tggtattcgg
      301 actctgcggc gaactggggt tcgacccgaa agaggcattg gtcgtcggcg atacggcgca
      361 cgacctgcat atggcggcaa acgcaggcgc ggcggcagtc ggcgtggcca ccggcgcaca
      421 ttcgcgcgaa cagctccgca ccgcaccgca tctcgccgta ttggacggtt tgtccgaact
      481 gcccggtttt cttgcacagc attacgcctg attggtttcc gcatcagaca ctcaaaacaa
      541 atgccgtctg aagcctgttc agacggcatt tgtgttgccc aaacattcaa cgcttgctca
      601 acgtttgcac aaatcgggtt tggtttcgcc ctcgcggcgc agctctttgg gcaggacgaa
      661 cacaatgctt tcttccgcac cctcgccttc gcgtacggtt tcgtgccccc atccgcgtat
      721 ggttgccagt acttcccgca ccaacacttc gggcgcggac gcgcctgccg ttacgccgac
      781 tttgtttttg ccctcaaacc atgcacgttg caggtagcct gcattatcca ccatatacgc
      841 atcgattccg cgcgatgccg ccacttcgcg caagcggttg ctgttggacg aattgggcga
      901 accgaccaca atcacgatgt cgcactgttc cgccaactct ttgacggcgg tttgccggtt
      961 ggtcgtcgca tagcagatat cttccttgtg cggattgcgg atattgggga aacgcgcgtt
     1021 cagcgcggcg atgatgtctt tggtttcatc gaccgagagc gtggtttggc tgacataggc
     1081 gagtttgtcg gggtttctga cttcgagttt tgccacatct ccgaccgttt cgaccaaaag
     1141 cattttgccc ggcgcaagct gccccatcgt gccttcgacc tcgacgtgcc ccttatgccc
     1201 gatcatgatg atttcacagt cttgggcatc cagtcgggcg acttccttat gcactttcgt
     1261 caccagcggg caagtcgcat caaacacgcg gaaaccacgt tcctccgctt cttgctgcac
     1321 cgccttcgat acgccgtgtg ccgaataaac cagtgtcgcg cccggcggca cttccgccaa
     1381 gtcttcaata aacaccgcac ctttttcacg caggttgtcc acgacgaatt tgttgtgaac
     1441 gacttcgtgg cgcacataaa tcggcgcgcc gaactcttcc aaagcacgtt cgacaatact
     1501 gattgcccga tccacaccag cgcagaagcc gcgcggattg gcaaggatga tggttttctc
     1561 gttcataagc ccggtatttc gttttcagac ggcatcaatg tttttcttct tgggttttac
     1621 ggtggacgat gttgtccaac actgccaaca ccgcaccgac gcagataaag ctgtcggcaa
     1681 tattaaaggc gggataaaac caattttgcc aataaaacaa taagaaatcg acgacatgac
     1741 cgtgtatcag gcggtcgatg acattgccta acgcaccgcc gataatcatc gccgcacccg
     1801 ttttgccgag ggttgcaaac tcatcgcgca agatggcgcg taccaaatac gcgctcaccg
     1861 ccaccgccag caccaaaaaa aagtattttt gccagccgcc ctgatcggca aggaagctga
     1921 acgccgcgcc cgggttgtac accaaggtca gatcgaaaaa cgaaggaatg acattgacgc
     1981 gttcccgata ctgaaacgac gacagcaccg cccacttcga ccactggtcc agcacgatgg
     2041 cggcaagtgc caatacccaa tagcgcgttt tacttgaaac agatgaagac atatttttca
     2101 acagccggta aaagagtacc attttacccg aaaaccccct ttcctgtacc cgaaacggca
     2161 aatgccgtaa tcttaaaacc cgtcattccc gacaacaccg taatctcgaa acccgtcatt
     2221 cccgcgtagg cgggaatcca gacctgtccg cacagaaact tatcggataa aaacagttgc
     2281 ccaaaccccg cgttctatag tggattaaat tcaaaccagt acggcattgc ctcgccttgc
     2341 cgtactattt gtactgtctg cggcttcgtt gccttgtcct gatttaaatt taatccacta
     2401 tagattccca cttccgtggg aatgacggtt cagttgcatt ccgacaacac cgtaatcttg
     2461 aaatccgtca ttcccgcgca ggcgggaatc tatcggaaat gactgaaacc tcgagattct
     2521 agattcccac tttcgtggga atgacggttc agttgcgttc caacaacacc gcaatctcga
     2581 aatccgtcat tcccgcgcag gcgggaatcc agacctccga cgcggcggga atctatcgga
     2641 aatgactgaa accccgagat tctagattcc cactttcgtg ggaatgacgg ttcagttgcg
     2701 ttccgacaac accgtaatct cgaaatccgt cattccc
//
LOCUS       DAWFAN010000191         2653 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWFAN010000191 DAWFAN010000000
VERSION     DAWFAN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.191"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(161..967)
                     /locus_tag="WGO65_001867"
     CDS             complement(161..967)
                     /locus_tag="WGO65_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ0328116.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1187..2557
                     /gene="ffh"
                     /locus_tag="WGO65_001868"
     CDS             1187..2557
                     /gene="ffh"
                     /locus_tag="WGO65_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ0328117.1"
                     /translation="MLDNLTSRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEITTAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          728 a          661 c          678 g          586 t
ORIGIN      
        1 gggatttttt tggggaaatt cgtcatgtcg ctgtcagata aggtttttta tttccaccaa
       61 acgctgcacc acccccaaca atccccctcc tctgactggt gcgcctttgt gaatatgccg
      121 tctgaaactt ggggcttcag acggcatctg ttggctcttc ttatcttttc agaatgattt
      181 ccaatacgaa cttgctgccc atataggcaa tcataaggct gacaaatccg atgatggtcc
      241 acacggcggc ttttttgccg cgccatgcgg tcatgctgtg cttgagcagc agtccgccgt
      301 aaatcagcca tgacaatatg ccgaatacgg ttttatgggt aaaggtcatg ggtttgccga
      361 atacggcttc ggcgaagaat gtgccgctga cgacggaata ggtcagcagg atgaaacctg
      421 cccacatggc ctggaacatg agtttttcca aactgagcaa cgacggcagg aatcctgcga
      481 gcttggagaa gctcctgcgg tgcaggctcc gattcagcag cagggtcaaa acggacaata
      541 atgttgcgat gccgaacagc ccgtatgcga gcagcgaagt tccgatatgc agcataaagg
      601 gaaggtcggt aatttcatat cccgagaatt ttccaggaaa aaccaaacct gacagcagca
      661 tcagtgcggc gcaaggatac agcagcaact gcactccgcg cagcggataa aaaaagctgc
      721 cggcaaaata aataaacagc atcatccaaa caatcaggct gccggaatac ccgaagccca
      781 taatgatgat tttgtcttga atgaccggca taagcagtgc cgcgccgtgg acggtcaatg
      841 ccgcgcccaa aaccggcaat tccgtcttcc acgggtaatc ccggccgcgc ccctgctgtt
      901 ggcagtgcca tgcaaatgca cccaatcctg cgtaaaccgc cgtcaaaaag atgaaaactg
      961 tcggcatggt ggactttctc tatctatact gttgcgccgt atgcggtcgc ttatgaaata
     1021 ttggaacttt taacgttgga attgtaaaat ccccgtttcg ggcaagcctt gacggatttg
     1081 ccgatatgct gtccggcaca caagccgcat caaattattt tgattttatt ttaacaaaga
     1141 atgcccctga tggggcaagc tattcttatt cagaccaagg accagtatgt tagacaattt
     1201 aaccagccgc ttcagcaatg tcttcaaaaa catccggggg caggccaaac tgaccgaaga
     1261 caatattaaa gaagccttgc gcgaagtccg cctcgccctg cttgaggcgg atgttgccct
     1321 gcctgtcgtc aaagagttcg tcaacaacgt caaagaaaag gccctcggtc aggaagtggc
     1381 gggcagcctg acgcctgacc aagcctttat cggcgtggtc aaccaagcct tagtcgaact
     1441 gatgggcaag gaaaacaaaa cgctggattt gtcggtttca ccgcccgccg tcgtgttgat
     1501 ggcgggtttg cagggtgccg gtaagacaac aaccgtcggc aaactcgccc gcctgttgaa
     1561 aaacgatcag aagaaaaagg ttttggtggt atccgccgac gtttaccgtc ctgccgcgat
     1621 tgaacaactg cgtctgttgg ccgaacaggt cggcgtggac ttttttccat ccgataccaa
     1681 ccaaaaaccg gttgagatta caactgccgc cgtcgattac gccaaaaaac atttttacga
     1741 tgtcttgatg gtcgataccg ccggtcgttt ggcaatcgat gaagagatga tgaacgaaat
     1801 caaagcgctt catgcggcgg tcaatccggt tgaaaccttg ttcgttattg atgccatgtt
     1861 gggtcaggat gcggtgaaca ctgctcaggc atttaatgaa gctctgccgc tgaccggtgt
     1921 cgtattgacc aagatggacg gcgactcgcg cggcggtgcg gcattgtccg tacgccatgt
     1981 aaccggcaaa ccgattaaat ttatcggcgt cggcgaaaaa atcaacggcc tcgaaccttt
     2041 ccaccccgac cgtcttgcca gccgcatcct cggcatgggc gacgtattga ccctgattga
     2101 agacgttcaa aaaggtattg atgaagaagc cgccgccaaa atggcgaaaa agctacaaaa
     2161 aggcaaaggc ttcgacctca atgactttaa agaacaaatc cagcagatgc gcaatatggg
     2221 cggcttggaa aacctgatgt cgaaaatgcc gggcgaactg ggtcaaatat cgaaacaaat
     2281 ccccgaagga acggctgaaa aagcgatggg caaagtggaa gccatcatca actcgatgac
     2341 tccaaaagaa cgcgccaacc ctgccctgct caaagccagc cgcaaacgcc gtattgcaat
     2401 gggtgcgggc acgaccgtac aggaagtgaa caaattgctc aaacagtttg aacaaatgca
     2461 acaaatgatg aagatgttca gcggcaacgg cttgggcaaa ctgatgcgta tggcgaaagg
     2521 aatgaaggga atgaaaggaa tgttcccggg tttgtaagcc gatttgaccg aaaacgccgt
     2581 ctaaaatttc agacggcgtt tttgttttat attctgattt atagtggatt aacaaaaatc
     2641 aggacaaggc ggc
//
LOCUS       DAWFAN010000192         2584 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWFAN010000192 DAWFAN010000000
VERSION     DAWFAN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.192"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(200..271)
                     /locus_tag="WGO65_001869"
     tRNA            complement(200..271)
                     /locus_tag="WGO65_001869"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(236..238),aa:Arg,seq:cct)
     gene            complement(291..737)
                     /locus_tag="WGO65_001870"
     CDS             complement(291..737)
                     /locus_tag="WGO65_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ0328118.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(742..1716)
                     /gene="ispB"
                     /locus_tag="WGO65_001871"
     CDS             complement(742..1716)
                     /gene="ispB"
                     /locus_tag="WGO65_001871"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ0328119.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVAVISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1940..2248
                     /gene="rplU"
                     /locus_tag="WGO65_001872"
     CDS             1940..2248
                     /gene="rplU"
                     /locus_tag="WGO65_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ0328120.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2273..2545
                     /gene="rpmA"
                     /locus_tag="WGO65_001873"
     CDS             2273..2545
                     /gene="rpmA"
                     /locus_tag="WGO65_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ0328121.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          757 a          696 c          574 g          557 t
ORIGIN      
        1 tatcataaaa caacagattt ccaagccgga acatctttta cggagcctga aaatcatttc
       61 atgattctac cgtcctaaag gtcggttttt caagcaggaa gaaaaagttt tcagatggca
      121 aaaaagccct ccaacactga aaggccttat atcggaaact tcccgcaaca cgggaaacag
      181 acaaatgcaa atcgtcaaac ctcgccaaca ggaatcgaac ctgtatttta cgcttaggag
      241 gcatacgttc tatccgttga actatggcga gccgaaatga aaaggagatt ttaacccttt
      301 ccgacgacaa aagacaagat gccggccgca atcagcggcc cgacagggat accgcccata
      361 aatgccaccc cgataaccgt cccgattaac agccctgtaa ttaaaacagg ctgctgtccc
      421 atcaaaggca cgccgcgtcc cgccagccaa gccacgaaaa taccgataaa aacggcggat
      481 atcattttaa aattcaaaaa ttcggcaacg ggaggaacct gcgcctttcc tgaaaccaac
      541 ggactcaaaa cccctatggt caaaagaatg ataccgagat tcaacccgtg cttctcgacc
      601 aacgggacaa actgtaccaa tgccgtctgc tgcatcagca gcaatatggt tgccgagatg
      661 gtaatcgaat tgttgttgct gaccaccccc aacagaatca gcgtaaccag aaacagggga
      721 acaaaactga aattcatcgc ctcaagacac cctgaccaaa gattccttcg ccagctgaat
      781 catggcatcc ttcacttcgc tgtcgggcag ggcatccaac gcggcaacgg cacaatcgac
      841 tgctttgcgc gcctcgccta tcgaatatgc caaagcatcc gaacggacga cataatcgtg
      901 gattttctca aaatagctgc gatctgcatt ttccaaagca gtacgcacat cgttcgcaac
      961 ctgttcggaa ccctgacgca tcagataaat caaaggcaaa gtcggttttc cttccgccaa
     1021 atcgtcgccg acgtttttgc cggtttcttc ggtttcgcca gaatagtcca gcacatcgtc
     1081 aataatttgg aatgccgtac cgacatacat accgtagtct ttcaacgccc gttcgtgttc
     1141 gggggaagcc ttgcccaaaa ttgcgccgac ttgagcggca gcttcaaaca attttgccgt
     1201 tttatattgg atgacttgga tatattgttc ttcggtaatg tccgtattgc cgatgttcat
     1261 cagctgcatg acttcgccct cggcaataat gttggttgca tccgccatca cttccaaaac
     1321 gcgcatactg cccgaggcaa ccatcagttg aaaggcgcgc gtgtataaaa agtcgccaac
     1381 caacactgcc gccgcattgc cgaacagatt gtttgccgtt gcccgcccac ggcgcaaatc
     1441 gctttcatcg acgacatcgt cgtgcaggag ggtggaagtg tggataaact cgaccatcgc
     1501 cgccagcgaa tacagtttct cgtcatcata accgaccgcc ttacccgcca aaatcgtcat
     1561 aatcggacgc aggcgtttgc cgcccgcgct gatgatatat gtaccgattt gcgaaatcac
     1621 tgcgacatcg gattgcaccg cacggttgat gacttcattg actttggcaa ggtcttcagg
     1681 cagatgtcgc tggaaatagg gcagattctc gagcatggca gactctcagg tttacgtttc
     1741 cgtcaaacgg cagataccgc ttgaaagctg attcaaatag cgggcaatta tatcagcaaa
     1801 gctatgagga cacatctttt cagacggcat atggttatcc gaaccgcgca aactttgaca
     1861 aaacaggcaa ataacaatac aatgccgagt tccgcacaat ggtgtgcgga tattaaccat
     1921 aacccttatg gagttgagta tgtacgcggt cgtaaaaacc ggcggcaaac agtataaagt
     1981 ttccgtcggc gaaaaattga aagtagaaca gataccagcc gaactcgaca gccaaatcga
     2041 actgaccgaa gttttgatga ttgctgacgg cgaatctgta aaagttggcg caccctttat
     2101 cgaaggtgca aaagtaacgg ctaaagtagt ggcacacggt cgtggcgaaa aagtccgcat
     2161 cttcaaaatg cgccgccgca aacactacca aaaacgccaa ggccaccgcc aaaatttcac
     2221 ccaaatcgaa atcgtggcaa tcgcctaacc ttaaaactta ggagcattac aaatggcaag
     2281 taaaaaagca ggcggcagca cccgcaacgg tcgcgattca gaagccaaac gcttgggcgt
     2341 taaagcctac ggcaacgagc tgattcccgc aggttccatc atcgtacgcc aacgcggtac
     2401 caaattccac gcaggcgaca acgtaggtat gggcaaagac cacactttgt tcgccaaagt
     2461 tgacggttat gtcgaattca aaaccaaagg cgcgctgaac cgtaaaactg tcagcatccg
     2521 tccttacact ggttctgaag aataatccaa ccgatacctg aagccgcatc ttttcacgat
     2581 gcgg
//
LOCUS       DAWFAN010000193         2580 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWFAN010000193 DAWFAN010000000
VERSION     DAWFAN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.193"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            76..705
                     /locus_tag="WGO65_001874"
     CDS             76..705
                     /locus_tag="WGO65_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ0328122.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPILLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            851..2512
                     /locus_tag="WGO65_001875"
     CDS             851..2512
                     /locus_tag="WGO65_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ0328123.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGHADFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT          504 a          654 c          673 g          749 t
ORIGIN      
        1 tgacggggtt gaagttgaca tctacaaggt ttaatgtttg aatacattta aaaatatatt
       61 tgaatgagat gttttatgat tcaaaatgtc gttacttcaa taatcctgta ttctgggaca
      121 gccgtagact tacttattat cctaatgtta ttttttgcca aaagaaaaag tagaaaagac
      181 atcattaaca tctatttagg acaatttcta ggctctgtta gtctgatatt gctaagttta
      241 ctttttgcat ttgtcttaga ttatattcct agtaaagaga ttttaggttt gctcggcttg
      301 attccaattc tcctaggcat caaagttttg cttttaggag attctgatgg agaggctatt
      361 gccaaagagg gtttgcgcaa agataataaa aacctgattt ttctagtcgc tatgattact
      421 tttgcaagtt gtggtgctga caatattggt gtctttgtcc catattttac taccttaaat
      481 ttagcgaatt tgatagtggc tttacttacc tttctagtca tgatttatct cttggttttt
      541 tctgcccaaa aattagcaca agtcccttct gttggagaaa ctttggaaaa atatagcaga
      601 tggtttgttg ctgttgttta tttgggattg ggggtatata tcctgattga aaacaacagt
      661 tttgatatgc tatggactgt gtcgggccag gaaaaaatat tatgaaaaag atagtatctg
      721 caaagactgc catgtgcagt cttttttgtt gccggtcttt ttgtgtctga tgccgtctga
      781 agcagtctct gcacgacctt tgtgcgaata tttgctacac ttggcaatcc gtccattttt
      841 tctcacgctt atgctgacct acactccgcc cgatgcccgc ccgcccgcca aaacccacga
      901 aaagccgtgg ctgctgctgt tgatggcgtt tgcctggttg tggcccggcg tgttttccca
      961 cgatttgtgg aatcctgccg aacctgccgt ctataccgcc gtcgaagcac tggcaggcag
     1021 ccccaccccc ttggttgccc atctgttcgg gcatgccgat ttcggcatac cacccgtgta
     1081 tctttgggtt gccgccgcgt tcaaacattt gctgtcgccg tgggcagccg acccgtatga
     1141 tgccgcacgc tttgcaggcg tgtttttcgc cgttgtcggg ctgacttcct gcggctttgc
     1201 tggcttcaac tttttgggca gacaccacgg gcgcagcgtc gtcctgattc tcatcggctg
     1261 tatcgggctg attccgaccg tacactttct caaccccgct gccgccgcct ttgccgccgc
     1321 cggactggtg ctgcacggtt attctttggc tcgccggcgc gtgattgccg cctcttttct
     1381 gctcggtacg ggctggacgc tgatgtcgtt ggcagcagct tatccggcag catttgccct
     1441 gatgctgccc ttgcccgtac tgatgttttt ccgtccgtgg caaagcaggc gtttgatgtt
     1501 gacggcagtc gcctcacttg cctttgccct gccgcttatg accgtttacc cgctgctctt
     1561 ggcaaaaacg cagcccgcgc tgttcgcgca atggctcgac tatcacgttt tcggtacgtt
     1621 cggcggcgtg cggcacgttc agacggcatt cagtttgttt tactatctga aaaacctgct
     1681 ttggtttgca ttgcctgcgc tgccgctggc ggtttggacg gtttgccgca cgcgcctgtt
     1741 ttcgaccgac tgggggattt tgggcattgt gtggatgctt gccgttttgg tgctgcttgc
     1801 cgtcaatccg cagcgttttc aggataacct cgtctggctg cttccgccgc ttgccctgtt
     1861 cggcgcggcg caactggaca gcctgaggcg cggcgcggcg gcgtttgtca actggttcgg
     1921 cattatggcg ttcggactgt ttgccgtgtt cctgtggacg ggctttttcg ccatgaatta
     1981 cggctggccc gccaagcttg ccgaacgcgc cgcctacttc agcccgtatt acgttcccga
     2041 catcgatccc attccgatgg cggttgccgt actgttcaca cccttgtggc tgtgggcgat
     2101 tacccgcaaa aacatacgcg gcaggcaggc ggttaccaac tgggcggcag gcgttaccct
     2161 gacctgggct ttgctgatga cgctgttcct gccgtggctg gacgcggcga aaagccacgc
     2221 gccggtcgtc cggagtatgg aggcatcgct ttccccggaa ttaaaacggg agctttcaga
     2281 cggcatcgag tgtatcgaca taggcggcgg cgacctgcac acgcggattg tttggacgca
     2341 gtacggcaca ttgccgcacc gcgtcggcga tgtacaatgc cgctaccgta tcgtccgctt
     2401 gccccaaaat gcggatgcgt cgcaaggctg gcagacggtt tggcagggtg cgcgcccgcg
     2461 caacaaagac agtaagttcg cactgatacg gaaaatcggg gaaaatatat aaaaaacaac
     2521 agattgagcc gaatttctgg attaagtgcc ggaaatcgcg tataattgcg cgattaaacc
//
LOCUS       DAWFAN010000194         2572 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWFAN010000194 DAWFAN010000000
VERSION     DAWFAN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.194"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..1134)
                     /locus_tag="WGO65_001876"
     CDS             complement(<1..1134)
                     /locus_tag="WGO65_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEZ0328124.1"
                     /translation="MRHSHYFQWLSLPLLSVAVTQQLYAQPNESLPTVELEPVVITID
                     KSGMALANRITQMPHTTKVIYEEQIQEQATGSRQLADVMAQLIPSLGVSSGTTSNFGQ
                     TMHGRQVQFLLNGVPLTGSRDISRQLNSINPNQVARIEVLSGATSIYGSGATGGLINI
                     VTKSDLEEEQFETRIGVHGSKLSSEGIGYQVGQSVAGVSENGNVLARLDVDYRTTGGA
                     FDANGKRIAPEPAQTDKQDSKSLSVNTNVDWQLDDKQNINLALTHYNDKQDTDYAPDY
                     GNRLAVLFEEKPSLNAIKGLSLSEQPKTTKSTFNINYHHDDLWGNTINTNAYYRREKG
                     RFYPFVAPFSTAKALPILQSMNLPPATLDAYRAYGVLQSESKAE"
     gene            complement(1181..2437)
                     /locus_tag="WGO65_001877"
     CDS             complement(1181..2437)
                     /locus_tag="WGO65_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0328125.1"
                     /translation="MIISQQKNRHLLTMVALLYVAHALPLYFFNVALPAILRHQGVDI
                     RVIGMLSLLYLPWAFKFLWASYVDNYYCAKIGKRKTWLFFTQIIVVVGLFGLGFLQFS
                     ESEIVLFLSISFLVSLASATQDIAIDGYTVEQFDKSSFGLASAMQSFGVAFGSMVGGA
                     GTLWLYQRFDWQMATISLATTVVLISCSLWFLKDQDNPKHATNLSRASLIRLIKRPQT
                     KWLLALIVVFRFVEAPMMAMLNPMLVDFGFSLSQIGLLFSVFGAIVGIGSALLAGVLI
                     KYIDIVRCLILFGWLRTAVYALLCLGLLYPLLGDFLPAVGLLKTGFMSLMAISVLLIL
                     SVRYLTMTALYALFMNAANPDQAGTDFTLFVCVELLVYFIGGAMSGFLVHQFGYDKLF
                     LWLAIGSGVSMLILPTLIKKFSVTDC"
BASE COUNT          819 a          588 c          482 g          683 t
ORIGIN      
        1 ctctgcctta gattcagatt gtaacacccc ataggcgcga taagcatcca aagtggctgg
       61 tggcaaattc atgctttgta aaataggcag ggctttggcg gtcgaaaacg gggcaacaaa
      121 gggataaaat ctgcctttct ctctgcgata ataagcattg gtattgatgg tgttacccca
      181 caaatcatca tgatgatagt tgatattaaa ggtgcttttg gtggtttttg gctgttctga
      241 taatgataag cctttgatgg catttaatga aggcttttct tcaaacaaca ccgcaaggcg
      301 attaccataa tcaggtgcat aatcggtgtc ttgtttgtcg ttataatgcg tcaatgccag
      361 attgatattt tgcttgtcgt caagttgcca atcaacattt gtattgacac ttaggctttt
      421 gctgtcttgc ttatcagttt gggcaggctc aggagcgatg cgtttaccgt tagcatcaaa
      481 tgcccctcct gtggtgcgat agtcgacatc aagtcgtgca aggacattac cattttcgct
      541 gacacctgct acactctgac ctacctgata accgatacct tcactggata atttactacc
      601 atgtacaccg atgcgggttt caaattgctc ctcttccaaa tcagacttag taacgatatt
      661 aatcaaaccg cctgttgctc cagacccata aatactggtt gctcctgata aaacttcaat
      721 tctagcaact tgattgggat tgatactatt aagctgtcta gagatgtctc gcgaacctgt
      781 caaaggcacg ccatttaaca aaaattgcac ttgacgaccg tgcatggttt gcccaaagtt
      841 actggtagtg ccactactta cccccaaact tggaatgagc tgtgccatca catcggcaag
      901 ctgtcgagag cctgttgctt gctcttgaat ttgctcttca taaataactt tggtggtatg
      961 gggcatttgc gtgatacgat tggcaagtgc cataccgctc ttatcaatgg taataaccac
     1021 aggctctaat tcaaccgttg gtaatgattc attgggttga gcgtacaact gctgagttac
     1081 tgccacactt agtaaaggca aagataacca ttgaaaatag tgggaatgac gcataaatta
     1141 ctccttatgg aaaagtaaaa ttttaacagt ctgttacggt ttaacagtct gttacggaaa
     1201 atttttttat caatgtcggc aaaataagca ttgacacccc agaaccaatc gcaagccaca
     1261 aaaatagctt gtcatagcca aactgatgta ctaaaaatcc actcatcgcc ccgccgataa
     1321 aatacacaag caactccacg cacacaaaca aagtaaagtc cgtgccagct tggtcgggat
     1381 tggcagcatt cataaacaga gcatataagg cagtcattgt taaatagcga acagataaaa
     1441 ttagcaaaac actaattgcc atcaagctca taaaccctgt ttttaataaa cctaccgcag
     1501 gtaaaaaatc acccaataaa ggatatagca atcctagaca caacaaagca taaactgccg
     1561 ttcttagcca tccgaataat ataagacaac gtacaatgtc gatatatttg attaataccc
     1621 ctgccaaaag tgctgaacca atcccaacaa tcgctccaaa taccgaaaac aaaagcccaa
     1681 tctgagacag tgaaaaacca aaatccacaa gcattggatt tagcattgcc atcatcggag
     1741 cttccacaaa gcgaaacacc acaatcaaag ctagtaacca tttagtttgc gggcgtttga
     1801 taagcctaat aagacttgct ctggaaagat tggtagcatg tttgggattg tcttgatctt
     1861 ttaagaacca tagtgaacac gaaataagca caacagttgt tgccaaactg attgtcgcca
     1921 tttgccaatc aaatctctga tatagccaca gtgtgccagc accgcctacc atactaccaa
     1981 acgccactcc aaaactttgc atggcacttg ccagcccaaa ggatgactta tcaaattgtt
     2041 ccactgtata gccatcaata gcgatatctt gggtggcact tgccaaagac accaaaaaac
     2101 ttatgctcag aaataataca atctcagact cagaaaactg taaaaagcca agcccaaaca
     2161 gcccgactac cacgataatt tgagtaaaaa ataaccaagt tttgcgtttg ccgattttgg
     2221 cacaataata attgtccaca taacttgccc acagaaattt aaacgcccac ggcaaatata
     2281 aaagtgaaag catgccaatc accctaatat caacgccttg atgtcttaaa atggcaggca
     2341 atgccacatt aaaaaaatac aaaggcaagg catgggcaac atataacagt gccaccatcg
     2401 tcaaaagatg gcgatttttt tgttgtgaga taatcatcag accgccccta ttttagtgtt
     2461 tatccatgct ttggcttcag tcagtgacaa caccaccgca taaggcactc gccatgcttt
     2521 atgaaaagca ggactgtcta aggcttgtct ttgagtctca tcatcaacaa tt
//
LOCUS       DAWFAN010000195         2535 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWFAN010000195 DAWFAN010000000
VERSION     DAWFAN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.195"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(227..1861)
                     /gene="groL"
                     /locus_tag="WGO65_001878"
     CDS             complement(227..1861)
                     /gene="groL"
                     /locus_tag="WGO65_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ0328126.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM"
     gene            complement(1954..2244)
                     /gene="groES"
                     /locus_tag="WGO65_001879"
     CDS             complement(1954..2244)
                     /gene="groES"
                     /locus_tag="WGO65_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ0328127.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGSRRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            2455..>2535
                     /locus_tag="WGO65_001880"
     CDS             2455..>2535
                     /locus_tag="WGO65_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0328128.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAPFFCT"
BASE COUNT          527 a          661 c          658 g          689 t
ORIGIN      
        1 ttcgcgggaa tgacggcgga aaggttgctg tttttccgat aaattcctgc cgctcttcgt
       61 ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca aaaaataaca atgcaaagaa
      121 cggtgttggc aattttaagg tttgcgcctt agccgcactt attcgcaaaa aaaccgcacg
      181 gcgttgaccg tgcggcttgt tgtctgaagg cttcagacgg cattgcttac atcatgccgc
      241 ccataccacc catgccgccc atatcaggca cagccggttt gtcttcgggg atttcagcga
      301 tcatgcaatc agtggtcagc atcaagccgg cgatagatgc ggcgtgttgc agcgcggaac
      361 gggttacttt ggcggggtcg agtacgccca tttcgatcat gtcgccgtat tcgccgctgc
      421 cagcgttgta accatagttg cctttgcctt ccaacacttt gttcacaacc acgctgggtt
      481 cgccgcctgc gttggcaacg atttggcgca gcggagactc aacggcgcgc aagacgattt
      541 gtacgcctgc gtcttggtcg gcattgccgg tgtgcaggtt ttccaaagca gcacgggcac
      601 gcaacagggc tacgccgccg cctgcaacca cgccttcttc aacggctgcg cgggtagcgt
      661 gcagcgcgtc ttccacgcgg tcttttttct ctttcatttc gacttcggtc gcggcaccga
      721 ctttgatgac tgccacgccg cctgccaatt tagccacgcg ctcttgcagt ttttctttgt
      781 cgtaatcgct ggttgcggtt tcgatttgtt ggcggatttc ggcaacacgc gcttcgattt
      841 gggctgcgtc gccaaagccg tcgatgatgg tggtgttttc tttaccgatt tcgatgcgtt
      901 tggcttgacc caagtcgtcc aaagtcgctt tttccaaaga cagaccgact tcttcggaaa
      961 tcaccacgcc gccggtcagg atggcgatgt cttgcaacat cgctttgcgg cggtcgccga
     1021 agcccggggc tttgacggca acggttttca ggatgcctcg gatgttgttc acgaccaaag
     1081 tcgccaaggc ttcgccttct acgtcttcag cgataatcaa cagcggacgg ctggctttcg
     1141 ccacttgttc caaaacaggc agcaagtcgc ggatgttgct gatttttttg tcgaacagca
     1201 atacaaacgg attgtccaaa gcagcgattt gtttttccgc gtcgttgatg aagtaaggag
     1261 acaggtagcc gcggtcgaac tgcatacctt caaccacatc cagctcgttt tccaaagatt
     1321 tgccgtcttc aacggtaatc acgccttctt tgccgacttt ttccatcgct tcggcgataa
     1381 tcgcgccgac ttgttcgtca gagttggcgg aaatcgagcc gacttgggcg atttctttag
     1441 aagtgtcgca aggtttggcg atgtttttca gctcgtcaac caaagcggca acggctttgt
     1501 cgataccgcg tttcaggtcg gtcgggttca taccggcggt aacgtatttc ataccttcgg
     1561 caacgatgga ttgcgccaat acggtggcgg tagtcgtacc gtcgcccgcc acgtcgttgg
     1621 ttttggacgc gacttctttc accatttgcg cgcccatatt ttcaaacttg tctttcagtt
     1681 cgatttcttt ggcgacggtt acgccgtctt tagtgatgtg cgggccgccg aatgcacggt
     1741 caacgactac gttgcgacct ttggggccca aggttacgcg gacggcgttt gccagaatgt
     1801 tcacgccgtt taccattttt tgacggactt cattgccgaa ttgtacgtct tttgctgcca
     1861 tttcaattct ccaaaaatca ttaaaactgt ctgataaaac cgtttatgcc gtctgaaggc
     1921 ggtttgccgt ttcagacggc atcgtgtccg tatttatttt tcaacgatgc cgaaaatatc
     1981 ctcttcgcgc atcaccaaca gctcttcgcc gtcggctttt acggtttgac cgctgtattt
     2041 gccgaagatg attttgtcgc cgactttaac atccagcgga cggcggctgc cgtctttgcc
     2101 gattttgccc gcacccacgg cgatgacttc gcccatatcg ggtttttcgg cggccgcacc
     2161 cggcaaaacg atgcccgatg cggttttttc ttcagcttcc aagcgtttga cgacgacgcg
     2221 gtcgtgtaaa ggacggatgg tcatatttat gctccgataa atggtttgaa aacaatcatc
     2281 tgcccgaacg gttcggacag attgaagtgg aaaccggaca gccgtcaggc ggctgcccgt
     2341 ataagtcggc aaaattaggg tgtgtgcggg caaattcaag tgaggcggaa aaaatttatt
     2401 tccgccgttt tttatagtgg actaaattta aggggctgta ctagattagc agatatgtta
     2461 ccctcgaaat atgaagataa cgcactgcaa attaaagaaa aaagtacaga aagaactgct
     2521 ccgttttttt gtact
//
LOCUS       DAWFAN010000196         2522 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWFAN010000196 DAWFAN010000000
VERSION     DAWFAN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.196"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..2329
                     /locus_tag="WGO65_001881"
     rRNA            <1..2329
                     /locus_tag="WGO65_001881"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2426..2518
                     /gene="rrf"
                     /locus_tag="WGO65_001882"
     rRNA            2426..2518
                     /gene="rrf"
                     /locus_tag="WGO65_001882"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          665 a          547 c          755 g          555 t
ORIGIN      
        1 ccttttgtat aatgggtcaa cgacttacat tcagtagcga gcttaaccga ataggggagg
       61 cgtagggaaa ccgagtctta atagggcgat gagttgctgg gtgtagaccc gaaaccgagt
      121 gatctatcca tggccaggtt gaaggtgccg taacaggtac tggaggaccg aacccacgca
      181 tgttgcaaaa tgcggggatg agctgtggat aggggtgaaa ggctaaacaa actcggagat
      241 agctggttct ccccgaaaac tatttaggta gtgcctcgag taagacactg atgggggtaa
      301 agcactgtta tggctagggg gttattgcaa cttaccaacc catggcaaac tcagaatacc
      361 atcaagtggt tcctcgggag acagacagcg ggtgctaacg tccgttgtca agagggaaac
      421 aacccagacc gccagctaag gtccccaatg atagattaag tggtaaacga agtgggaagg
      481 cccagacagc caggatgttg gcttagaagc agccatcatt taaagaaagc gtaatagctc
      541 actggtcgag tcgtcctgcg cggaagatgt aacggggctc aaatctataa ccgaagctgc
      601 ggatgccggt ttaccggcat ggtaggggag cgttctgtag gccgatgaag gtgcattgta
      661 aagtgtgctg gaggtatcag aagtgcgaat gttgacatga gtagcgataa agcgggtgaa
      721 aagcccgctc gccgaaagcc caaggtttcc tgcgcaacgt tcatcggcgt agggtgagtc
      781 ggcccctaag gcgaggcaga aatgcgtagt cgatgggaaa caggttaata ttcctgtact
      841 tgattcaaat gcgatgtggg gacggagaag gttaggttgg caagctgttg gaatagcttg
      901 tttaagccgg taggtggaag acttaggcaa atccgggtct tcttaacacc gagaagtgac
      961 gacgagtgtc tacggacacg aagtaaccga taccacgctt ccaggaaaag ccactaagct
     1021 tcagtttgaa tcgaaccgta ccgcaaaccg acacaggtgg gcaggatgag aattctaagg
     1081 cgcttgagag aactcaggag aaggaactcg gcaaattgat accgtaactt cgggagaagg
     1141 tatgccctct aaggttaagg acttgctccg taagccccgg agggtcgcag agaataggtg
     1201 gctgcgactg tttattaaaa acacagcact ctgctaacac gaaagtggac gtatagggtg
     1261 tgacgcctgc ccggtgctgg aaggttaatt gaagatgtga gagcatcgga tcgaagcccc
     1321 agtaaacggc ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt
     1381 tccgacccgc acgaatggcg taacgatggc cacactgtct cctcctgaga ctcagcgaag
     1441 ttgaagtggt tgtgaagatg caatctaccc gctgctagac ggaaagaccc cgtgaacctt
     1501 tactgtagct ttgcattgga ctttgaagtc acttgtgtag gataggtggg aggcttagaa
     1561 gcagagacgc cagtctctgc ggagccgtcc ttgaaatacc accctggtgt ctttgaggtt
     1621 ctaacccaga cccgtcatcc gggtcgggga ccgtgcatgg taggcagttt gactggggcg
     1681 gtctcctccc aaagcgtaac ggaggagttc gaaggttacc taggtccggt cggaaatcgg
     1741 actgatagtg caatggcaaa aggtagctta actgcgagac cgacaagtcg agcaggtgcg
     1801 aaagcaggac atagtgatcc ggtggttctg tatggaaggg ccatcgctca acggataaaa
     1861 ggtactccgg ggataacagg ctgattccgc ccaagagttc atatcgacgg cggagtttgg
     1921 cacctcgatg tcggctcatc acatcctggg gctgtagtcg gtcccaaggg tatggctgtt
     1981 cgccatttaa agtggtacgt gagctgggtt taaaacgtcg tgagacagtt tggtccctat
     2041 ctgcagtggg cgttggaagt ttgacggggg ctgctcctag tacgagagga ccggagtgga
     2101 cgaacctctg gtgtaccggt tgtaacgcca gttgcatagc cgggtagcta agttcggaag
     2161 agataagcgc tgaaagcatc taagcgcgaa actcgcctga agatgagact tcccttgcgg
     2221 tttaaccgca ctaaagagtc gttcgagacc aggacgttga taggtggggt gtggaagcgc
     2281 ggtaacgcgt gaagctaacc catactaatt gctcgtgagg cttgactcta tcatttgaag
     2341 aacttcaaga gataaaagct tactgactga ttcagtcatt accgaatata ttgattaagg
     2401 ctttaccgat ttgtaacagt ttaagtttgg cggccatagc gagttggtcc cacgccttcc
     2461 catcccgaac aggaccgtga aacgactcag cgccgatgat agtgtggttc ttccatgcga
     2521 aa
//
LOCUS       DAWFAN010000197         2484 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWFAN010000197 DAWFAN010000000
VERSION     DAWFAN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.197"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            35..1390
                     /locus_tag="WGO65_001883"
     CDS             35..1390
                     /locus_tag="WGO65_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ0328129.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2413
                     /locus_tag="WGO65_001884"
     CDS             1394..2413
                     /locus_tag="WGO65_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ0328130.1"
                     /translation="MSDTPATPATRDFGLIDGRAVTSYVLSNRRGTRVCVLDLGGIVQ
                     EFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEAN
                     EGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDR
                     LTVSYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDL
                     EVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDR
                     NGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYG
                     IESLS"
BASE COUNT          457 a          674 c          747 g          606 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 aaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta ccaagaagcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccccgct acccgcgatt tcggcctgat
     1441 cgacgggcgt gccgtaacca gctatgtgct gtccaatcgg cgtggtacgc gtgtctgcgt
     1501 gctggacttg ggcgggattg tgcaggaatt ttccgttttg gcagacggcg tgcgcgaaaa
     1561 cctcgtggtg tcgttcgacg atgcggcttc ctatgcggac aatccgtttc agattaacaa
     1621 gcagataggg cgcgtggccg gacgcatccg cggtgcggcg ttcgacatca acggcaggac
     1681 ttaccgcgtg gaggccaacg aaggcaggaa cgcgctgcac ggcggttcgc acgggctggc
     1741 cgttacccgt ttcaacgcgg tggcggcaga cggccgttcg gtggtgctgc gcagccgcct
     1801 gcaacagtcg gccgacggtt atcccaacga tttggatttg gatatttcct accgcttgga
     1861 cgaggacgac cggcttaccg ttagctatcg cgccaccgcg ctcggcgaca cggtgttcga
     1921 cccgacgctg cacatttact ggcggctgga cgcgggcctg cacgatgcgg ttctgcatat
     1981 tccgcagggc ggacatattc cggccgatgc cgaaaaactg cccgtctcaa cggtttcaga
     2041 cgacctcgaa gtatttgatt tcagccggcc caagccgctg gatgccgccg ttgccgccct
     2101 gcgccgcgaa acgggtcggg ccggttttga cgacgcttac cgcgtgccgt ccgatatagg
     2161 ccgtcccgcc gctgtgttgc aagccggacg ccgccgtcgt atcagcatat acagcgaccg
     2221 caacggcttg gtcatcttta ccgccgcccc gcaggatttc gcgcggcacg atgcgggcgt
     2281 ttacgacgcg ctggcgaccg aggcgcagac gctgcccgac agcctgaatt ggcccgagtt
     2341 cggcaatatt cgtctgaaca agggtgatac tagggaggcg acgattgctt acggcatcga
     2401 atccctttct taggagcttc ctaacaccgg ttgcagacgg cctttttata gtggattaac
     2461 aaaaaccggt acggcgttgc ctcg
//
LOCUS       DAWFAN010000198         2451 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWFAN010000198 DAWFAN010000000
VERSION     DAWFAN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.198"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            25..555
                     /locus_tag="WGO65_001885"
     CDS             25..555
                     /locus_tag="WGO65_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0328131.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKW"
     gene            697..2358
                     /locus_tag="WGO65_001886"
     CDS             697..2358
                     /locus_tag="WGO65_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ0328132.1"
                     /translation="MTESITRDNMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     VTPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHKDGSVKGIATGNM
                     GVGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVMHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIAYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMINVNYKEYASPETRYCPAGVYEIIE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT          621 a          601 c          678 g          551 t
ORIGIN      
        1 cctgttgctt atggaaaaat caaagtgcag agttggaaag agaggcgcga tttcaatatt
       61 gtaaagcagg atttggattt ttcctgcggg gcggcttcgg tggcgacgct tttgaacaat
      121 ttttacgggc aaaagctgac ggaagaggac gtgttaaaaa aactggataa ggagcagatg
      181 cccgcgtcgt ttgaggatat gcggcgcatt atgcctgatt tgggttttga agcgaaaggc
      241 tatgccctgt ctttcgagca gcttgcacag ttgcaaatcc ccgtcatcgt gtatctgaaa
      301 taccgcaagg atgatcattt ctcggtattg cgcggtatag acggcaatac ggttttgctt
      361 gccgacccgt cgctgggtca tgtttcgatg agcagggcgc agtttttgga tgcttggcaa
      421 acccgtgagg gaaatttggc aggtaagatt ttggcggttg tgccgaaagg gagggatgcc
      481 ggcggggata aggcgttttt cacacgcagt ccgaaacgtc agacggagtt tgcggtcgga
      541 caggtaaaat ggtagcgtgc ttattgaaat ttcgacaaag gtcgcctgaa aaccgaaaat
      601 atggttttca gacgaccttt gttgtatttg gtaactatat gttcccgttg tataattacg
      661 gatttgcaat tcaataataa atacacagga accgccatga cagaatccat cacccgcgac
      721 aatatgcaat acgatgtcgt gattgtcggc gcaggcccgt cgggtttgtc cgccgccatc
      781 aaactcaagc agcttgccga acaaaatggg cgcgaaatca gcgtttgcgt ggtggagaaa
      841 ggttcggagg cgggtgcaca ttcgctggcg ggcgcggtta tcgatccgat tgcgctgaat
      901 gagttgattc ccgactggaa agaaaaaggc gcgccgctga cacgcacagt aacgcaggac
      961 aaggtgttgt tcctgaccga gaaaaaagcg ttcaacctgc ccgttacccc caatttcgac
     1021 aatcatggca actacatcgt cagcttgggc gaagtcgtac gctggttggc ggagcaggcg
     1081 gaaaatttgg gcgtggaaat ttatccgggc tttgccgctg ccgaagtgct gtatcacaaa
     1141 gacggttcgg tcaaaggcat tgcaaccggc aatatgggcg tgggcaaaga cggcgaaccg
     1201 accgattcat tccagcccgg catggagctt tgggcacagc aaaccctgtt tgccgaaggt
     1261 tgccgaggtt cgctttccaa gcaaatcatc gaacgcttca aactcgacca aaacagccag
     1321 ccgcaaactt acggcttggg cattaaagaa gtttgggaag tgccgtccga acagcatcag
     1381 cccggtttgg tgatgcacag cgcaggctgg ccgctggaca gcaaaaccta cggcggcgcg
     1441 tttgtttacc atttcgacga caacaaagtt gccgtcggct tcgtggtcgg tttggactat
     1501 caaaatcctt atctgtcgcc gtttgaagag ttccaacgtt tcaaaaccca tcccgaaatc
     1561 cgcaaaacct ttgaaggcgg ccgccgcatt gcttacggcg cgcgttcgct gattgaaggc
     1621 ggtttgcaaa gtttgccgaa gctctcgttc aaaggcggca ttttggtcgg cgatgcggcg
     1681 ggtttcctca atatgccgcg catcaaaggc attcataccg cgatgaaatc cgccatgctc
     1741 gccgccgaag ccgtgttccc tttgttggaa aaccttgaag aagtggaggg tttcgacagc
     1801 ggcaaagagg cggcggatta tcagcaacgt tttgaacaaa gctggctgta tcaagagctt
     1861 tacgccgcgc gcaacgtccg tccgtcattc aaatggggcg tttacctcgg ctcgctttat
     1921 accggcatcg accagatgat tttcagaggc aaagccccgt ggaccttgaa acatcacggt
     1981 aaagacaacg agcagctcaa aaaagccgcc gcgtgcaagc cgattgctta tccgaaaccc
     2041 gatggcgtgt tgaccttcga ccgcttgagc agcgtcttcc ttgccaatct cgcgcacgaa
     2101 gaaaaccagc ccgaccattt ggtgctgaac aatccgcaaa cgatgataaa cgtgaactac
     2161 aaagaatacg cctcgcccga aacgcgctat tgtccggcgg gtgtgtacga aattattgaa
     2221 gaaaacggca atccgcgcct gcaaatcaac gcggcaaact gcgtgcactg caaaacctgc
     2281 gacatcaaag acccgacgca aaacatcact tggatttgcc ccgaaggcgc gagcgggccg
     2341 aattacggcg ggatgtaaac cttattccgc cgttacgccc ggtcaggaat aaggaatggg
     2401 gcagttgttc aaacctgccc cgtttgaacc cataatatag tggattaaac t
//
LOCUS       DAWFAN010000199         2450 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWFAN010000199 DAWFAN010000000
VERSION     DAWFAN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.199"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(136..1269)
                     /locus_tag="WGO65_001887"
     CDS             complement(136..1269)
                     /locus_tag="WGO65_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328133.1"
                     /translation="MFKKFKLVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDKLPDFPLNLENLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(1443..1892)
                     /locus_tag="WGO65_001888"
     CDS             complement(1443..1892)
                     /locus_tag="WGO65_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328134.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENEIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCNISHFLHFADRA
                     EADIPNQSRSYAEYWWEMEMFKMSVLEDWERSGRPLNWEKWQRQYRS"
     gene            complement(2058..2381)
                     /locus_tag="WGO65_001889"
     CDS             complement(2058..2381)
                     /locus_tag="WGO65_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328135.1"
                     /translation="MNILASDRNYELNIEIFIDDIIHFQITDESYKVKFSEYLFENKT
                     ENNSDGNPTANYFFEIIDDSYMDWLKEESFGFFEKKYYKAYIFFFSDSVIEVISSTEP
                     VFYSK"
BASE COUNT          667 a          602 c          427 g          754 t
ORIGIN      
        1 ccgtttaatg tgtatttccg ttttttggat tgtggttttc aatttgtagc gaatcggatt
       61 cggcatatac ggcattgcaa aaagcgtttg actctccaat gccgtctgaa aaccggtttc
      121 agacggcatt tgcgttcagt gagaaaggtc gcgcctgccg cccgaacgtc tcgccgcagc
      181 ctctgcataa cggctcacct ctttttccaa attttccaag ttcaaaggaa aatcaggcag
      241 tttgtctccc tgtttctctt cgcggataat ccgcccgcca tccaaatacc acgtctgttg
      301 cgcatgatag gtctgtatat ccgccgttac gccatccgct ttcaatgcta ccgtcgaaga
      361 ttgtgcaata aaaagatttc cgtttttcaa ataatattcg aaactctggc gtttttttcc
      421 attgtcgaaa ctccaataga ctttttgcgg cagaccgtcc gcatcatagc cgaccacaag
      481 actgttcgcc ttcatccccc ggggcatcaa ttcccgcata ttctgataaa acacagaatc
      541 gcgcgagtcc gatgcaatcc ggttgctctc ttcgcggaag tcccaaacct tctgctcatc
      601 attcgcgaca tcccggtatt tcgccaaata tacctgggcc atctgataac acccgaggca
      661 atgctcataa acatcttccc cgattttccc gcgccccgac gcatcaaata ccgaaccgtc
      721 cggttgccaa acaacccgat attctcctgt cgtttcataa ttttctccgt gagccgttcc
      781 gccgtacaca tttacagaaa acggacgatc gttccgatac agatattcgg cattaacaaa
      841 cgcttccggc gagcgttgcg aaagcgaaac cgcaaccaaa ccgccctcgc cgatatggta
      901 atccagccaa acctctttcc catgttcctg ctccgttacg tgaaaccatt tcgccttttc
      961 tttcaaacga ctgagccgga tagcgagcgc gagataatcc ttctccgact gcaacggacc
     1021 gtcatccaca gctccggcaa gactttcctc cgtcctcatc gattccttca cgatgacaac
     1081 cgccctgtcg gcatttcgga acaggcgggc aagtttcgcc acaaaagcat tcggattttc
     1141 aggtatttca gttgccgtat cgctcaaaaa ccaacgcgga ttaatctcat aggcaatacc
     1201 cgtccccagc caaaaggcaa atacaagggc aaaaaatgac aacaatacca gtttgaattt
     1261 tttaaacata tttatttttc gtttaattcg tttaacagga tatatcgatt atatcagacg
     1321 agctttgatt gtttgatttt taatctaatc tacgcttgct gccgtacttt aaaaaaacct
     1381 atgcaggctg cggcttgtta ctcttgtttg ctttgattca aattttcaac taattcaaat
     1441 gcttaagatc gatattgcct ttgccatttt tcccaattta acggtcttcc cgagcgttcc
     1501 caatcttcta aaaccgacat tttgaacatt tccatttccc accaatattc ggcatatgaa
     1561 cgtgattgat tgggaatgtc ggcttctgcc cgatcggcaa aatgcaaaaa atgagaaata
     1621 ttgcaaacta tattattttc aggaacttct tcggcaaata gatgcttgac tgactgtaac
     1681 gcttcggtct gatatttttc tgtccatttc tcccattttt caggctttcc cgatatttcc
     1741 caatcttcca atgcggaagc attcaagatt tccaattccc accaacagtc gtaataacgg
     1801 gaggattggt taggaatctc attttctgct tcggaaacaa attgggtaaa actaaaaata
     1861 tccttaacga ttttttcaaa tctattttcc ataggttgcc gcccctccca attgcgtgcc
     1921 aacaatttcg gcaaaatcaa gccggcaagc cccggcatcc tgtgctaatc ggagaaaatg
     1981 ccgtctgaaa gccgaatacc gcttcagacg gcattttgat tgttgggttt ttaatcagag
     2041 ctttgtttga taatttgtta ttttgaataa aatacaggtt ctgtcgagct gataacttct
     2101 attacagaat cgctaaaaaa gaaaatgtaa gccttataat atttcttttc aaaaaaacca
     2161 aaactttctt ctttcaacca gtccatataa ctgtcatcta ttatctcgaa aaaataattt
     2221 gcagtaggat ttccatccga attattttct gttttatttt cgaataaata ttctgaaaat
     2281 tttactttat aagattcatc tgttatttga aaatgaatta tatcgtcaat aaatatttca
     2341 atatttaatt cataatttcg gtcggatgcc aaaatgttca catcttgttc ggtaacacaa
     2401 attttgtcca cataaaatct gtgattattg ttagaaaatt gtaatggttt
//
LOCUS       DAWFAN010000200         2393 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWFAN010000200 DAWFAN010000000
VERSION     DAWFAN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.200"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            570..947
                     /locus_tag="WGO65_001890"
     CDS             570..947
                     /locus_tag="WGO65_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0328136.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            1036..1605
                     /gene="rsmD"
                     /locus_tag="WGO65_001891"
     CDS             1036..1605
                     /gene="rsmD"
                     /locus_tag="WGO65_001891"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ0328137.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            1728..1979
                     /locus_tag="WGO65_001892"
     CDS             1728..1979
                     /locus_tag="WGO65_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ0328138.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            2076..2159
                     /locus_tag="WGO65_001893"
     tRNA            2076..2159
                     /locus_tag="WGO65_001893"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2110..2112,aa:Tyr,seq:gta)
     gene            2190..2263
                     /locus_tag="WGO65_001894"
     tRNA            2190..2263
                     /locus_tag="WGO65_001894"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2222..2224,aa:Gly,seq:tcc)
     gene            2273..2347
                     /locus_tag="WGO65_001895"
     tRNA            2273..2347
                     /locus_tag="WGO65_001895"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2305..2307,aa:Thr,seq:ggt)
BASE COUNT          725 a          494 c          563 g          611 t
ORIGIN      
        1 cgcacctata aaagcttttt cattacgttc agcatgacgg tcacgtcgtt catatttttt
       61 acgcttgctg ttccctgtta ttacagctaa gccaagtgat atggcgagaa ttgcccaaac
      121 aatagtactt aaataacaat tttccccata ctatcaataa ggaaagaaaa aaccttttag
      181 tattaggtcg atagattata atccatgccc atgaaaatgc aataagagtt atacataaga
      241 tacaagctga caggatttct ttatttttaa ttaaataact tagagcgatg aggatgacag
      301 gtgtcaagaa aataatagtt acatcccgat agctataaaa gaaaactgcc ctattttgaa
      361 tgtggagatg tgcacagaat ccaatatagc taaggataat agttaatata ataaaaaaag
      421 accaccaagg gtgaagagat aggaattcca tgttttccct ttttttgtaa aaaggaaaaa
      481 atcttatcta atagttaatt gcctaaccag ccagaagaag tttaaaatct atcccaataa
      541 ttcaaccatc tatacagaaa gttcagctta tggaaaccca cgaaaaaatc cgcctgatgc
      601 gcgaattgaa taaatggtcc caggaggata tggcggaaaa gctggcgatg tcggcaggag
      661 ggtatgccaa aatcgaacgg ggcgaaacgc agttgaatat cccgcgtttg gagcagttgg
      721 ctcagatttt caaaatcgat atgtgggact tgctcaaatc gggcggtggt gggatggtgt
      781 ttcagattaa tgaaggtgat agtggtggcg atattgcgtt gtatgcgtcg ggtgatgttt
      841 cgatgaaaat agaattttta aaaatggagt tgaaacactg caaagaaatg ttggaacaaa
      901 aagacaaaga aatcgagctg ctccgcaagc tgaccgaaac cgtttaaaca gatatgccgt
      961 ctgaaaaaag ttttcagacg gcatattctt tgacaggtct tgtataatac cgtttgaact
     1021 tacaggtttt tgattatggc ggcaggcaaa cataccaaac acagcaaccg ggtacgcatt
     1081 atcggcgggc aatgccgggg caggaaattg agtttcacat ccgccgacgg actgcgtccg
     1141 acacccgaca gcgtgcgtga aaagctgttt aactggctgg gacaggattt gacgggtaaa
     1201 acggttttgg atctcttcgg aggcagcggc gcactcggta tagaagccgc ttcgcgcaac
     1261 gccaaacgcg tgctgatttc ggataacaac cgccaaaccg tgcagacctt gcagaaaaac
     1321 agtcgcgaac tgggtttggg gcaggtgcaa atcgtctttt cagacggcat cgcatatttg
     1381 aagaccgtat ccgaacagtt tgatgttgtc tttctcgacc cgccgtttgc atggcaggac
     1441 tggcaaatcc tgtttgatgc cttgaagccg tgcctgaacc cccgggcatt cgtctatctc
     1501 gaggcgggta cgctgccgaa tattcccgat tggctgacgg aatatagaga agggaaatcg
     1561 gggcagagta catttgaatt aagggttttc caagtggctg aataatatgc gctttgataa
     1621 tcatttccga gttgtaaaca ttcgtttgca accgtccggt tcaaaaaaac cttgtgctat
     1681 aatccgcgcc cgcccggttt tgataattta gtggaaaagg aaaagaaatg tcgcttttta
     1741 ttaccgacga gtgcatcaac tgcgacgtat gcgaacccga atgccccaat gatgccattt
     1801 cccaaggcga ggaaatttac gaaatcaacc ccaacctctg cacgcagtgc gtcggacact
     1861 acgatgagcc gcagtgccag caggtttgcc cggtggactg catcctgatt gacgaagaac
     1921 atcccgaaac ccatgacgag ttgatggcga aatacgaaaa gattatccag tttaaataaa
     1981 ttctttttaa aacatcaaat tatgtctgtt ttgaaataaa atcaaaaaaa aacttgacgg
     2041 aaaagcaagc cgctaataaa ctaacgttct cttttggagg gattcccgag cggtcaaagg
     2101 gggcagactg taaatctgtt gcgaaagctt cgaaggttcg aatccttctc cctccaccaa
     2161 aattcttact tggggcagta gcgagtaatg cgggtgtagc tcaatggtag agcagaagcc
     2221 ttccaagctt acggtgaggg ttcgattccc ttcacccgct ccaaacaatt aggcccatgt
     2281 agctcagggg tagagcactc ccttggtaag ggagaggccg gcagttcaaa tctgcccatg
     2341 ggcaccatct ctcgattatt catttcttta aggcttagat atataggata ttg
//
LOCUS       DAWFAN010000201         2374 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWFAN010000201 DAWFAN010000000
VERSION     DAWFAN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.201"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..1071
                     /locus_tag="WGO65_001896"
     CDS             <1..1071
                     /locus_tag="WGO65_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0328139.1"
                     /translation="TFFDKYGVRVSLLEDEDFIRAASMLAEEVFGFGIYKESKGSGGR
                     FYERCWLMGSEDVLYGRVHFGGQNNTILFELTGTGCGVAKEGWESRLFAFLTNAIRPK
                     ITRVDIAKDFFNGEYSPNQAREDRNKGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKT
                     YGIGSRESSKYVRVYEKGKQLGDKTSTWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAY
                     PICERFVQKATRIHAVKEDKVISADRYLEWVKKQFGRAANGLKFIFPELDKAKLFELI
                     EPSHHKLPKSLAPEAYDCAFLKAQAIHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHV
                     NNEESYNNFIYDKFARLPISWA"
     gene            1116..1406
                     /locus_tag="WGO65_001897"
     CDS             1116..1406
                     /locus_tag="WGO65_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328140.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGRSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1411..1608
                     /locus_tag="WGO65_001898"
     CDS             1411..1608
                     /locus_tag="WGO65_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328141.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            1616..1900
                     /locus_tag="WGO65_001899"
     CDS             1616..1900
                     /locus_tag="WGO65_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328142.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            1907..2185
                     /locus_tag="WGO65_001900"
     CDS             1907..2185
                     /locus_tag="WGO65_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328143.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSLKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
BASE COUNT          709 a          542 c          542 g          581 t
ORIGIN      
        1 acctttttcg ataaatacgg cgttcgtgta agtcttttgg aagacgaaga tttcattcgt
       61 gccgcgtcca tgctcgccga agaagttttc ggtttcggta tctacaaaga atccaaaggt
      121 tcgggcggtc gtttctatga gcgttgttgg ttgatgggtt cggaagacgt cctatacggt
      181 cgcgtccatt ttggcggaca aaacaatacc atccttttcg aactgaccgg caccggttgc
      241 ggcgtcgcaa aagaaggctg ggaatcacga cttttcgcat tcctgactaa tgcaatccgc
      301 ccaaaaatca cacgcgttga catcgcaaaa gactttttca acggcgaata cagcccgaac
      361 caagcccgtg aagaccgaaa taaaggtatg tttacctgtc atcacgtcaa accaaaaggc
      421 gaatgtttgg ggtcagattg ggaagaagac gatgaagcca aaatgaccaa aggcaagacc
      481 tatggtatcg gctcccgtga atcgtccaaa tatgtccgcg tctatgaaaa aggcaagcag
      541 ttgggcgata aaacaagcac atggacgcgc tttgaaattg aattcaaagc aaaagacatc
      601 gttatccctt ttgaagtttt gcagaatccg ggcgaatatt tcggcggcgc atatccgatt
      661 tgcgaacgat tcgtccaaaa ggcaacgcgc atacacgcgg ttaaggaaga taaggtcatt
      721 tcagccgacc gctaccttga atgggtaaaa aaacagtttg gacgtgcggc aaacggtctg
      781 aaattcattt ttcccgaatt ggacaaagcc aaactgtttg aactgattga gccgagtcat
      841 cacaagctgc ccaagtcttt ggctcccgaa gcctacgact gcgccttttt gaaagctcaa
      901 gccattcatg aacagcccgc attcaaaccg tacaaagacc cttactatat gtacgaatat
      961 tacgagaatc ttgaaaaaca gcttgaacag caaaaacacg tcaacaatga agaaagctat
     1021 aacaacttca tttacgacaa attcgcaaga ctaccgattt catgggctta aagtgtctgt
     1081 ccgaaagacg tttaatcaca caaggaaacc aaaaaatgaa catccaactt caaggccaca
     1141 tcgtcggcgt taaaaaattc aacggacaaa tcgaaggcag gagcttcgac tattgccgcc
     1201 tgattgtcgc cacaccctta gacagctccc aaggcaacgc attgggcagc tctactactg
     1261 aatacgattt cggcggctct gccaatttcg agcagttccg aaacgcccaa tttccgatcg
     1321 aagcaaacct gaacgtagaa atcgtcacta cgggcaaaac ccaaaaactg aaagtcatcg
     1381 gttttcaact cgtgaagaaa ggctgattga atgcagaaag tctatgttgt ccagtccgta
     1441 tcaacagggg actttctgta tctctctcct gaaacgggcg acatcggaca taccaaatta
     1501 atcaccaatg ccgattattt ctacgacttc gaagaagcga ttaacgcagg tttggaagaa
     1561 atcggcaacc aatacgaatt tgtcgtattc ggatttttga aagactgatt ttcggatgtt
     1621 cggcggtcgt ttgaaaaacg ctccatccat taccgccaaa cactttttga aggaaaatat
     1681 catgaaattt attaacacct gccgtaaata cggcgcaaaa ctggctgttg taacagctgc
     1741 tcccctggct ttggccgcac atgcaaatgc aacgttgccc gatacggcaa aaaacgcttt
     1801 ggaagccgca aaagcggacg gtatggaagc cggttggatt gtcgttggcg ttttcgccgc
     1861 gctttttgta ttttccatcg ttaagagagt gatgaagtaa gacggcatgt actaccaagt
     1921 cggaaataaa tgtcttgaga agcaccaggc tgaaaacctt tatttcagct tggtagtacc
     1981 aagaatcaaa gaaaacggac agattgtcag gccggaatat aacggcagcc tgtggaagat
     2041 gtcggacggt cagccgctaa ggcttttatt ggcggaatgc agtctgaaag acaacctgca
     2101 aagcggcctt gaaacaggct ggatagtatt cggcatcctc gcgtccgttt actttgtttc
     2161 cctgctgaaa aaggttttga aatgatggat ttttattttt atctcggcgt ttccgtaccc
     2221 gtattaatcg gggcggttct gtttaagaat tgagcgcatg aagttatggt gtcaaaatca
     2281 ggctttcaaa acaatcattg aaaggcagaa ccatgaacaa gccgtttatc actcaggcgc
     2341 agttggcact ttataaatat cagccgtcta gcaa
//
LOCUS       DAWFAN010000202         2351 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWFAN010000202 DAWFAN010000000
VERSION     DAWFAN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.202"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            271..2151
                     /locus_tag="WGO65_001901"
     CDS             271..2151
                     /locus_tag="WGO65_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ0328144.1"
                     /translation="MNIFYEESGQFKVAAVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKHGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGIKRVGIHIAAPS
                     LAIKQGGKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPAVSIYFDVDSK
                     FNVGEPTCKIEAVNIAENLRIQTIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAITL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          635 a          705 c          556 g          455 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagccgcgcc aaccctcccc
       61 aaaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgtt tcaacacaca ggacggcaca taaagcaccg ccctatgtgt
      181 tgccctgatt tggaaggggt tgcgcccctc ccaaataaat tctgattcta ccgccctaaa
      241 ggttggggtt tcaaccgaaa aggaaacacg atgaatatct tttacgaaga gtccggccaa
      301 ttcaaagtcg ccgccgtcgt ccaaaaaaac gatgccacct accaagtcga tacccaacac
      361 ggcaaacgca ccaaagtgaa ggcaaacaat gtctttgccg agtttgacgg cgatatggcg
      421 gcgtttttgg aaaacgcgca ggcgcaggcg gcggacatcg acaccgattt attgtgggaa
      481 gtatgcggcg aagaggaatt taccgccgaa gccatcgccg aagaatatta cggtcatgcg
      541 ccgaccaaaa ccgagctggc ggcaactttg attgcacttt acgccgcgcc ggtgtatttc
      601 tacaaaaaag ccaaaggcgt gttcaaagcc gcgcccgaag aaaccttaaa acaagcgctt
      661 gccgccatcg aacgcaaaaa acagcaggac gcacaaatcg acgcttgggc agaagccttg
      721 aaacacggcg agatgccgtc tgaaatcgcg gcggatttga aaaccatcct acacgcgccc
      781 gacaagcagt cgctgaccta caaagccttt accaaagccg ccgacgcgct gaaaacctcc
      841 gcctacgaac tggcgaaaaa aacgggcggc attacgtcca ttccgcaata cctgcaagac
      901 gggtttgaaa tcaaatactt tcctaaaggc acaggcttcc ccgacctccc ccttcccgaa
      961 atgcccgacc tgcccaaggc tgatgttacc gccttttcca ttgacgacga atcgaccacc
     1021 gaagtggacg acgctttgag cctgaccgac ttgggcaacg gcataaagcg cgtcggtatc
     1081 catatcgccg cgccgtcgct tgccattaaa caaggcggca aaatggaaaa aaacatcatg
     1141 gaacgcctga gtacggtcta tttccccggc ggaaaaatta ccatgctacc cgaaaactgg
     1201 attgccgcgt tcagccttga tgcaggtgca taccgccctg ccgtcagcat ttatttcgat
     1261 gtggacagca aattcaacgt cggcgagccg acctgtaaaa tcgaagcggt caacatcgcc
     1321 gaaaacctgc gcattcaaac catcgagccg catttcaacg ccgaagcggg cttggacgaa
     1381 gcgggcgaaa tgatgttcgc ccaccatcaa gacctgattt ggttttatca attcgccatc
     1441 accctgcaaa aagcgcgcgg caaatacgaa cccgaccgcg cgccgcaata cgattacagc
     1501 atcgaattgg atgaggaagg caaagtctcc gtcgtccgcc gcgaacgcgg ttcgcccatc
     1561 gatacgctgg tcagcgagat gatgattctt gccaacagca cttgggcaca aatgctccat
     1621 gacaacgacc tgcccggcct cttccgcgtc caacccaccg gaaaagtacg catgagcacc
     1681 aaatccgagc cgcacatcgg catgggcgtg cagcattacg gctggttcac ttcgccgctg
     1741 cgccgcgccg ccgactacat caaccaaaag cagctgatca gcctgataga cgacactgcc
     1801 gagccgctgt atcaaaacag cgatgccgag cttttcgccg cgctgcgcga ctttgatgcc
     1861 gcctataccg cctacgccga cttccaacgc caaatggaag cctactggag cctggtttac
     1921 ctgcaacagc aaggcatcag cgagctgacg gcgacgattc tgaaagaaga cctcgtccgc
     1981 atcgaaggcc tgccgctggt cacgcgcgcg accggtattc cgtttgacgc actgcccaaa
     2041 tcgcaggcat tgttcaaaat caccgaatta gatgccgaga agcagtttgt ctcgctcaac
     2101 tacatcaagg cagtcgcacc cgcgggtaca acggcaggca atgccgtctg aagcccgata
     2161 cggcaaactc cggcaaaaac agaaacctga atctcatcat tcccacagtc gggaatccgg
     2221 ttttttgggt ttccgttgtt tttccgtttc aatgaacttc caagccgccg ttcccgataa
     2281 atacccgcaa tctaaaatcc cgtcattccc acgcagatgg gaatctaggt ttatccgcac
     2341 agaaactgat a
//
LOCUS       DAWFAN010000203         2338 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWFAN010000203 DAWFAN010000000
VERSION     DAWFAN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.203"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(61..648)
                     /locus_tag="WGO65_001902"
     CDS             complement(61..648)
                     /locus_tag="WGO65_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ0328145.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSDGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            811..2217
                     /gene="dnaB"
                     /locus_tag="WGO65_001903"
     CDS             811..2217
                     /gene="dnaB"
                     /locus_tag="WGO65_001903"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ0328146.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          578 a          603 c          620 g          537 t
ORIGIN      
        1 aataactgaa attcaatacc gcaaaaatct acccgaaatg atatagcgga ttaacaaaaa
       61 tcaggacaag gcggcaaaac gtttggcaac ttcgtcccag ttgacgattt cccagaaacc
      121 tttcaggtag ttgggacggc tgttgcggta gtcgatgtaa taggcgtgtt cccacacgtc
      181 gcaggtcagc agcggcgtgt tttcagtggt cagcggcgta gcggcgttgg aagtggaaac
      241 caaatccaat ccgccggcag gggtttttac cagccacgcc caaccggagc cgaaagtacc
      301 ggccgcgcag gcattgaacg cttcttggaa tttctcgaag ctgccccatt tcgcgtcgat
      361 ggcggcggcc agttcgccgg aaggtttgcc ttgacctttg ggcgtgaaac ccagccagta
      421 gaaggtgtgg ttccaagttt gcgccgcgtt gttgaacacg ccgtccgaag attttttcac
      481 aatctcttcc aaaggcaggt tttcaaattc ggtgcctttg atttgattgt tcaggttggt
      541 gatgtaggtt tgatggtgtt tgccgtagtg gaattccaaa gtctctttgc tcagatgcgg
      601 ggacaatgcg tccagttcat aaggcagttg cggcagctta tgttccattt tgtgctccta
      661 atattgtttt aaatgttgta ttttggcagt gttgctgcaa atagctcagc agcgcacata
      721 ttttacctgt tttgagggat ggaaaccaat taaacctgct ttacgctata atagaagatt
      781 gcaatttcgg cacgacagat aggatgtacc atgaacgatt acgcagctat gccgtctgaa
      841 gaccgtgagg tcggcgcatt gtcgctgcct ccacattcga tggaggcgga acaatccgtt
      901 ttgggcgggt tgatgctgga aaatccggcc tgggacagga ttgccgatgt ggtttcggga
      961 gaggatttct accgccacga acaccgcctg attttccgat ccattgccaa attaatcaat
     1021 gagagccgtc ccgccgatgt gattacggtt caggaagatt tgcagcggaa cgaagaattg
     1081 gaagcggcag gcggattcga atatctgatt acgctggcgc aaaacacccc gtctgccgcc
     1141 aacatccgcc gctacgccga aatcgtgcgc gagcgttcca tcatgcgcca actcgccgaa
     1201 gtggggacgg aaatcgcccg cagcgcatac aatccgcaag gcagggacgc ggggcagctt
     1261 ttggacgagg cggaaaacaa agtattccaa atcgccgaaa gcaccgccaa atccaagcag
     1321 ggctttttgg agatgcccga tttgctgaaa gaagtcgtac agcgcatcga tatgctctac
     1381 tcgcgcgaca atcccgatga agttaccggc gtgccgacag ggttcatcga cctcgacaaa
     1441 aaaacctcgg gtctgcaacc cggcgacctg attatcgttg ccggtcgtcc gtctatgggt
     1501 aagaccgcct tttccatcaa tatcgccgaa cacgttgccg tagaaggcaa gctgccggtt
     1561 gccgtcttct caatggagat gggcggtgcg caactggtca tgcgtatgct cggctcggtc
     1621 ggacgcttgg atcaaagcgt cctgaaaacc ggcaggctcg aagacgaaca ctggggtcgt
     1681 ctgaacgaag cagtcgtcaa actctccgac gcgcccgtgt acatcgacga gaccccgggt
     1741 ttgactgcgc tcgaactgcg cgcccgcgcc cgccgtctcg cccgtcaatt taacaataag
     1801 ctgggattga tcgtcatcga ctacctgcaa ctgatggcgg gatccggccg ttccgacaac
     1861 cgagcttcag agctgggcga aatttcacgt tcgctcaaag cgttggcgaa agaattgcaa
     1921 gtccccatca tcgccctgtc gcaactgagc cgcacggtcg aatcgcgtac cgacaaacgc
     1981 ccgatgatgt ccgaccttcg cgaatccggt gctatcgagc aggatgccga cctgattatg
     2041 tttatgtacc gcgacgaata ctacaaccaa gactcgccca tgaaaggcct tgccgaatgt
     2101 atcatcggca aacaccgcaa cggtcccgtc ggtaaaatct tcctcacatg gacggggcaa
     2161 ttcaccaaat tcgacaatgc tgcctatatt cccgaggagg caaagataga ggattaaatg
     2221 gctatataaa aatttattag gcgaaatcag gcaaaatcgt ttaaaatcat gcttagagat
     2281 tgccctaaaa aataaaacgc ggtcttgagg catttttgca ttcagcccgc atataatt
//
LOCUS       DAWFAN010000204         2338 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWFAN010000204 DAWFAN010000000
VERSION     DAWFAN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.204"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            43..2256
                     /locus_tag="WGO65_001904"
     CDS             43..2256
                     /locus_tag="WGO65_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0328147.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNVPDNPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          598 a          755 c          566 g          419 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac gaaagaccca tcatgaccgc catcagcccg
       61 attcaagaca cgcaaagcgc gactctgcaa gaattgcgcg aatggttcga cagctactgc
      121 gccgcgctgc cggacaacga taaaaaactt gtcttagccg cccgttcgct ggcggaagca
      181 cattaccccg ccgatgccgt cacgccgtat ggcgagccgt tgtccgacca cttcctcggc
      241 gcggcacaaa tggtcgacga actcgacctg ctgcccgatg ccgtcgccgc caccctgctt
      301 gccgacatcg gacgctacgt ccccgattgg aatctattgg tttccgaacg ctgcaacagc
      361 accgtcgccg agctggtcaa aggtgtggac gaagtgcaga aactcaccca cttcgcccgg
      421 gtggacagcc tcgccacgcc agaagaacgc gcccagcagg cagaaactat gcggaaaatg
      481 ctgctggcga tggttaccga catccgcgtc gtgttaatca aactggcgat gcgtacgcgc
      541 accctgcaat tcttaagcaa cgttcccgac aatcctgaaa agcgcgccgt tgctaaagaa
      601 accctcgaca tttttgcccc actcgccaac cgcttgggcg tgtggcaact caaatggcag
      661 ctcgaagatt tgggcttccg tcatcaagag ccggaaaaat accgcgaaat cgccctgctt
      721 ttggacgaaa aacgcaccga gcgcctcgaa tacatcgaaa acttcctcaa tatcctgcgt
      781 acggaactca aaaaatacaa tatccacttt gaagtcgccg gccgcccgaa acacatctac
      841 tccatttaca aaaaaatggt gaagaaaaaa ctcagcttcg acggcctctt tgacatccgc
      901 gccgtgcgga ttctggttga taccgtcccc gagtgttaca ccacgctggg cattgtccac
      961 agcctttggc agcctatccc cggcgagttc gacgactaca tcgccaaccc gaaaggcaac
     1021 ggctataaaa gtttgcacac cgtcatcgtc ggtcccgaag acaaaggtgt ggaagtgcaa
     1081 atccgcacct tcgatatgca ccaattcaac gaattcggtg tcgccgccca ctggcgttac
     1141 aaagaaggcg gcaaaggcga ttccgcctac gagcaaaaaa tcgcctggtt gcgccaactt
     1201 ttggactggc gcgaaaatat ggcggaaagc ggcaaggaag acctcgccgc cgccttcaaa
     1261 accgagcttt tcaacgacac gatttatgtc ctgaccccgc acggcaaagt cctctccctg
     1321 cccacgggcg cgacccccat cgacttcgcc tacgccctgc acagcagcat cggcgaccgt
     1381 tgccgcggtg cgaaagtcga agggcagatt gtgccgctgt ccaccccgct cgaaaacgga
     1441 cagcgcgtcg aaatcattac cgccaaagaa gggcatcctt ccgtcaactg gctttacgaa
     1501 ggctgggtca aatccaacaa ggcaatcggc aaaatccgcg cctacatccg ccagcaaaac
     1561 gccgacaccg tgcgcgaaga aggccgcgtc caactcgaca aacagcttgc caaactcacg
     1621 cccaaaccca acctgcaaga gcttgccgaa aatctcggct acaaaaagcc agaagacctc
     1681 tacaccgccg tcggacaagg cgaaatttcc aaccgcgcca tccaaaaagc ctgcggcacg
     1741 ctgaacgaac cgccgcccgt acccgtcagc gaaaccacca tcgtcaaaca gtccaaaatc
     1801 aaaaaaggcg gcaaaaacgg cgtgctcatc gacggcgaag acggtctgat gaccacgctt
     1861 gccaaatgct gcaaacccgc gccgcccgac gacattgtcg gcttcgttac ccgcgatcgc
     1921 ggcatttcgg tacaccgcaa aacctgcccc tctttccgac acctcgccga acacgcgccc
     1981 gaaaaagtac tggacgcaag ttgggcggcg ttgcaggaag gacaagtgtt cgccgtcgat
     2041 atcgaaatcc gcgcccaaga ccgctccggg cttttgcgcg acgtatccga cgcgctcgcc
     2101 cgccacaaac tcaacgttac cgccgtgcaa acccagtccc gcgacttgga agccagtatg
     2161 cggttcacgc tcgaagtcaa acaggtcaac gacctcccgc gcgtcctcgc cagcctcggc
     2221 gacgtcaaag gcgtattgag cgttacccgg ctttaaatac aaaaatgccg tctgaaagcc
     2281 gtataacgct tcagacggca ttttgattga cggggtttgc tattttttgt tgcatagt
//
LOCUS       DAWFAN010000205         2334 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWFAN010000205 DAWFAN010000000
VERSION     DAWFAN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.205"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            30..1247
                     /locus_tag="WGO65_001905"
     CDS             30..1247
                     /locus_tag="WGO65_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0328148.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTAIAIIGLANIAGCVF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            complement(1450..1590)
                     /locus_tag="WGO65_001906"
     CDS             complement(1450..1590)
                     /locus_tag="WGO65_001906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328149.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1603..>2334
                     /locus_tag="WGO65_001907"
     CDS             1603..>2334
                     /locus_tag="WGO65_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEZ0328150.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKGKYPDTVFTALGAIDKS"
BASE COUNT          545 a          724 c          539 g          526 t
ORIGIN      
        1 gcaaacttaa ccgactcaaa atacacatta tgacgcacac cgcatcgaaa acacccaaat
       61 tttggctgac catcaccgcc gccgcattca tcctgctgat taccatcggc atgaggatga
      121 cgctcggact gttcgtccaa cccgtcgtca acaccaccga attgagcatt gcccaattca
      181 gcctcatcat cgccgttttc caactgatgt ggggcatatc gcaaccattg tccggcgcgc
      241 ttgccgaccg tttcggcgcg ttcagggtat taagcggcgg cgccgtcctg ctcgtctgcg
      301 cctgcctgat tgcccccaac atcccgactt attgggggct gatgattgcc gtcgggctgc
      361 tgctcgcatt cggcaccggt tccggcggtt tctccatcat tatggggcag gtcgccgccc
      421 aagtccccgc acacaaacgc ggcttggcat ccggactggt caacgccggc ggttcggcag
      481 gacaattcct gttcgcaccg ctggttcaag gactcgtcgt cctgcccgaa gtcggctgga
      541 cgggcacatt ttacgtttgg ggcgcaatcg ccctgctgat tctgcctgtc tcatggtggc
      601 ttgccggcgg caacaacgcc gcccacaccc aacacgccca agccacacac ggacaaagcc
      661 tcggagaagc agtcaaaacc gccttcaaaa ccccaagcta catcctgctg cacctaagct
      721 tttttgcctg cggcttccac atcgcctttc tcgtaaccca cctacccacg gaagtcgccc
      781 tgtgcggact gcccgccacc gtcgcatcca cagccatcgc catcatcgga ctggcaaaca
      841 tcgccggttg tgtgttctca ggctggtgca caagccgcgt tcggggcaaa tacgtcctgt
      901 tcggactgta cgcctcgcgc gccgcgatga tactcatcta cattttttcg cccaagaccg
      961 acctcaactt ctacattttc gccgccgcac tcggatttac ctggctcgcc accgtcacgc
     1021 cgaccgcctc cattaccggc aaactcttcg gcacacgcta cctcgccacc ctgttcggac
     1081 tgacgatgct cagccaccaa atcggcggat tcctcggctc gtacatcggc ggcatcgtga
     1141 ttacacaatt cggcgactac ggctggatgt ggtacgccga cgcactgctg gcgggaaccg
     1201 ccgccctcct caacctcccc atccgcgaac cccgaaccgc cgcatgaaag aatgccgtct
     1261 gaaaaccctc agacggcatt ttccaaacac cccgcccgtt tccggactgc acgacacgca
     1321 aatcagcctt atcaaaccgc aaacaaccgc cgcgattaaa ccgccattca aataagtccg
     1381 ttttcccttc gggtgtaaaa tcatcacaaa acgcgatact gtatatcgcc gggcgttatg
     1441 cagcggattt caggataaaa tgccgtctga agccgcccct gcccgggcgg gcgaagcgtc
     1501 ggatgcggtg cgcttggcaa aggtttgccc gccgcgcccc gtatccggca tttcatcaat
     1561 gcagaccgca tttccaatcc tataattcaa ggtgtccgcc ttatgaaaat cgtttttatc
     1621 acaacagtcg catccagcat ttacggtttc cgcgcccccg tcattaaaaa attaatcggc
     1681 aaaaaccatc aggtgtatgc ctttgtatcg gagttttccg ataatgaatt ggatattatc
     1741 agggaaatgg gggttacacc cgttacctac cgttcaaacc gcagcgggct gaacccgttt
     1801 tccgatataa aatccacctt cctcatcttt aaagaactca aaaaaatatc gccggatttg
     1861 gttttccctt atttcgcaaa acccgtgatt ttcggcactt ttgccgcaaa actggcaggc
     1921 gtgcccagaa tcgtcgggat gctggaaggt ttgggattcg catttacccc gcagccggaa
     1981 ggcataccgt taaaaacaaa aatcataaag gggattttga ttgccttgta ccacattgcc
     2041 ctgccgatgt tggaaagcct gattgtatta aaccccgacg acaaagacga actgacggac
     2101 aaatacggca tcaaaataaa aaacatccat attttgggcg gaatcggtct ggatttgcgg
     2161 caatatcctt attccgaggc ggatattccc gatgaaaaag aacccgtaaa atttctcttt
     2221 atcggcagat ttctgaaaga aaaagggatt gatgatttta ttcgggcggc ggaacaggtt
     2281 aagggcaaat accccgatac ggtttttacc gctttgggcg caatcgacaa atca
//
LOCUS       DAWFAN010000206         2319 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWFAN010000206 DAWFAN010000000
VERSION     DAWFAN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.206"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WGO65_001908"
     CDS             complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WGO65_001908"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ0328151.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTINDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLQITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGNGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMVVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1627..2193
                     /locus_tag="WGO65_001909"
     CDS             1627..2193
                     /locus_tag="WGO65_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0328152.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          526 a          565 c          670 g          558 t
ORIGIN      
        1 tcctagttat ctaggacagc cccttaaatt taatccacta tattttcctg tttcaggctc
       61 ggcgttcttt gcacatcagt ttcgcatcca gccagccatc gccgccggag atgatgttgt
      121 tggcaacgaa gttgtccaag tcgggcagca gggaaacggc gcgaccgctg ttgatgatga
      181 cgaccaccac catcggtttg tcgcccagcc agtagcctgc caatgcgcgg acattgttga
      241 gtgtgcctgt tttcaggcgc agaagcccac cgctttgttt gaagcggttg cgtaaagttc
      301 cgtctgtgcc ggcgatgggc agcgtgtcga tgaaatcttg tgcaaacggg ctgaaataag
      361 ccgtttccaa catttgcgcc atcattctcg ccgttaccct ttcttttctg gacagacccg
      421 aaccgttttc caaaaccaaa tccgcaacat cgatgcccga cacggcaagt tcgcgccgga
      481 cggcagatgc cgcctgttcg gaaacggcgg gcagtttgcc gttgccgccg agtttgagga
      541 agacggaacg cgcaatcaga ttgtccgaac gcttgttcat gtccgtcaaa atttccttca
      601 tcggtttggc gtgtgcaacg gcaagcgtct gcgcgccttc cggcgtgtcg gctatgccga
      661 taccgtctga aatccgtccg ccgccgagca gccagtggtt ggtaaaactt tgccggatca
      721 gttcgtcaag cgcgaacatc cggacaccga caggcttgcc caaacagctc tcgggaatat
      781 tgccgcgcaa tttcagcgta ttgtccgaaa aagatgcacg catcagtttt ttgatcgaag
      841 ggcaggcagc ttgggaggcg gtaatttgca agttgttttg ggcgaaaata tgcggcaaag
      901 gcggatcggt gaggatgtcg gtactgtcgg cggcattgcg ttcggcgcgc accataacca
      961 taccggcaga cagcatagtt ggattggggg gcgtcataaa cggcgaaccg ctgtcggctt
     1021 cgaaatcgtc ggggctgccg acttcgcccc acaggctgtg gtcgagcatc aggtgtcccg
     1081 tgatattgcg tatgccttgt tcgcgcaact ggcgttggac ggcaagcagg ttttcctgat
     1141 tgaaaacggg gtcgccgctg cccgcccaat acaggtttcc gtcaagcgtg ccgtcgttta
     1201 tcgtaccgtt gcttttaaac tcggtcgccc agcggtaatt gctgccgaag gttttgaagg
     1261 cggcaaacgc ggtaacgagt ttcattgtgg aggcggggtt gacggggaca tccgagcggt
     1321 ggtcaatgat gacttttccg ctgtcaagct cttggacata tacggcgatt tcgttttgcg
     1381 gaatgcggcc ggtatcgagc gcgtgtgcgg cgagggaggc gagaagcagc agcagggagg
     1441 ccgctgtttt ggggaaattc ataggtaaat ctgttacata aaaaaagcaa ttataaggca
     1501 aagccggata agtgggaacg ctgtggcggc gggccggctt gtttgcaggg gaaatcacat
     1561 atataataat cgctatcatt atgaaatgat tgagacgcac aaacttaata ttcaggagga
     1621 atgattatgg ctaagaaaat cagtattttg gtgggcagcc tgcgccgtgc ttcttttgcg
     1681 cgcaaagtgg cattgaatgc ggcggagatg ttccccgaag gctggcaggc ggaaatcgtc
     1741 gaaatcggac atctgccgct ttacaatttc gattatgacg accctgcggt ggaagatgtg
     1801 ccgctgcccg aaagctacac ggctttccgc gaaacgatta aggcttcgga cggcattttg
     1861 ttcgttacgt ccgaaaataa ccgcaccatt cccgcctgtt tgaaaaatgc ggtggacatc
     1921 ggctcgaaac cgaatgccga cgtggcgtgg aaaaacaaac cggccggtat catcagccat
     1981 tccgtcggca agatgggcgg ttacagttcg caaaaaaacc tgcgccttgc gctgtcttat
     2041 ttcgatatgc ccgtaaccgg acagccggaa gtgtttttgg gcaattcgcc gacgctgttt
     2101 gatgaaaacg gcaaattgat cgactcggca agggattttg ttcagtcata tattaatcag
     2161 tttgttggtt tgattgaaag aaatgccaaa taaacaaacc aagtcgaaag gccgcaaaac
     2221 cggttcgggt tttgcggctt ttttatatgg gctgggcagc gtcaaggttt gccgtttgcc
     2281 ggataatgcg cggcaaatga ccggcgtgtt tatagcgga
//
LOCUS       DAWFAN010000207         2296 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWFAN010000207 DAWFAN010000000
VERSION     DAWFAN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.207"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(35..991)
                     /locus_tag="WGO65_001910"
     CDS             complement(35..991)
                     /locus_tag="WGO65_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0328153.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            1227..2192
                     /locus_tag="WGO65_001911"
     CDS             1227..2192
                     /locus_tag="WGO65_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0328154.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAGKE"
BASE COUNT          486 a          617 c          734 g          459 t
ORIGIN      
        1 gtttcaaatc aaaatatcta tgccgccggc cccatcagca cttcttcaca tccgaaggca
       61 aaaatccgta atgccgtctg aacgcttcgt tgaaccgtcc cgcgtggcgg tagccgcaaa
      121 agtgcatggc ggcttggacg gtgctgccgg attcgatgag ggcgagcgcg tgttccagcc
      181 gcaggcggcg caggcatccg gcgacggttt cgccggtttg cgctttgaaa tagcgtttca
      241 ggtagcattc gttcagtccg acgcggcggg cgatttcggc gatggtcagc ggacgggcga
      301 attcgtgttg caggatgtcg gcggcttcgt ctatgcgccg acggcggtaa ccgttgtcgt
      361 ggcggcggaa ggtgaagcgc aataatcggg cggagagttc cagcgcggcg gcttcgtcgg
      421 caagcaggcc gaagccgtcc gattcgaacg ggcgttgcag cagtgggcag gccgccgccg
      481 tcagtgccgc cgcgttttgc gccagccgtt gcagggcgaa tcggcctatc gtttgcggcg
      541 aaaacaggcg ttcgtccagc aagccttcgt cgtgccagcg gcgcagtttt tccagcgaaa
      601 aatccaaatg cagcgcgcac atgccgctgt tgtcgggcag cagggtttcg gatacgtccg
      661 ccaactcgcc gcgtaccagc cagatttcgc cggcagacgg gcggtattcc ctgccgccca
      721 tttgtaaccg gttctgcccc gacaccatga cgaacaaggc gcagttgtgg ctgaaattgt
      781 ggatttcggt gggaaacgcg cccgttccgc cgccgcgcat ccgcgacaag gtgatgcccg
      841 agtcgaagcg gttgatgcac atttccagat gcaaaccggg ctgttttgcc tgcgcaatga
      901 gcgcgctgtc ggaacagccg tccaacgccc agccggattt atcggagcgg acataggttt
      961 ggtactggcg gtagatggcg gcggtgttca tgattggata ggaacgagtt gtctaacaaa
     1021 tgaattaaat aggaattatt accaataatc aagcgcaggg attggttgaa acggaaaagg
     1081 tcgtctgaaa gggtgtttca gacgaccttt tccgtatcgg gaatttgttt tgccgtatcg
     1141 ggaattttgc gttttgcggc gtggtttctg caggttgttt gcttaataat aaacattctt
     1201 attcgtatgc aaaggaaccg cacaccgtga aaccgcgttt ttattgggca gcctgcgccg
     1261 tcctgctgac cgcctgttcg cccgaacctg ccgccgaaaa aactgtatcc gccgcatccg
     1321 catctgccgc cacactgacc gtgccgaccg cgcggggcga tgccgttgtg ccgaagaatc
     1381 ccgaacgcgt cgccgtgtac gactgggcgg cgttggatac gctgaccgaa ttgggcgtga
     1441 atgtgggcgc aaccaccgcg ccggtgcgcg tggattattt gcagcctgca tttgacaagg
     1501 cggcaacggt ggggacgctg ttcgagcccg attacgaagc cctgcaccgc tacaatcctc
     1561 agcttgtcat taccggcggg ccgggcgcgg aagcgtatga acagttggcg aaaaacgcga
     1621 ccaccataga tctgacggtg gacaacggca atatccgcac cagcggcgaa aagcagatgg
     1681 agaccttggc gcggattttc ggcaaggaag cgcgcgcggc ggaattgaag gcgcagattg
     1741 acgcgctgtt cgcccaaacg cgcgaagccg ccaaaggcaa aggacgcggg ctggtgctgt
     1801 cggttacggg caacaaggtg tccgccttcg gcacgcagtc gcggttggca agttggatac
     1861 acggcgacat cggcctaccg cctgtagacg aatctttacg caacgagggg cacgggcagc
     1921 ctgtttcctt cgaatacatc aaagagaaaa accccgattg gattttcatc atcgaccgta
     1981 ccgccgccat cgggcaggaa gggccggcgg ctgtcgaagt attggataac gcgctggtac
     2041 gcggcacgaa cgcttggaag cgcaagcaaa tcatcgtcat gcctgccgcg aactacattg
     2101 tcgcgggcgg cgcgcggcag ttgattcagg cggcggagca gttgaaggcg gcgtttgaaa
     2161 aggcagaacc cgttgcggcg gggaaagagt agggagtcgt ctgaaaacgg agcttccgaa
     2221 ggaagcgggg ggtttctgcg aagctaaagt gcggtttcaa cgaattgaaa agcagcctgt
     2281 atgttgaaaa taccgc
//
LOCUS       DAWFAN010000208         2282 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWFAN010000208 DAWFAN010000000
VERSION     DAWFAN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.208"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            234..2012
                     /locus_tag="WGO65_001912"
     CDS             234..2012
                     /locus_tag="WGO65_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ0328155.1"
                     /translation="MNKIYRIIWNSALNAWVAVSELTRNHTKRASATVKTAVLATLLF
                     ATVQANATDEDEEEELESVQRSVVGSIQASMEGSGELETISLSMTNDSKEFVDPYIVV
                     TLKAGDNLKIKQNTNENTNASSFTYSLKKDLTGLINVETEKLSFGANGKKVNIISDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDMLLNTGATTNVTNDNVTDDEKKRAASVKDV
                     LNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKTEVKIGA
                     KTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSV
                     SLGAGADAPTLSVDDKGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
     gene            2145..>2282
                     /locus_tag="WGO65_001913"
     CDS             2145..>2282
                     /locus_tag="WGO65_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0328156.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YL"
BASE COUNT          698 a          566 c          562 g          456 t
ORIGIN      
        1 ccgccgtcat tcccgcaaaa gcgggaatcc agacccgtcg gcacggaaac ttaccgaata
       61 aaacagtttc cttagattcc acgtcccaga ttcccgcctt cgcgggaatg acgagatttt
      121 aagttggggg aatttatcag aaaaccccca acccccaaaa accgggcgga tgccgcacca
      181 tccgccccca aaccccgatt taaccattca aacaaaccaa aagaaaaaac aaaatgaaca
      241 aaatataccg catcatttgg aatagtgccc tcaatgcctg ggtcgccgta tccgagctca
      301 cacgcaacca caccaaacgc gcctccgcaa ccgtgaagac cgccgtattg gcgacactgt
      361 tgtttgcaac ggttcaggcg aatgctaccg atgaagatga agaagaagag ttagaatccg
      421 tacaacgctc tgtcgtaggg agcattcaag ccagtatgga aggcagcggc gaattggaaa
      481 cgatatcatt atcaatgact aacgacagca aggaatttgt agacccatac atagtagtta
      541 ccctcaaagc cggcgacaac ctgaaaatca aacaaaacac caatgaaaac accaatgcca
      601 gtagcttcac ctactcgctg aaaaaagacc tcacaggcct gatcaatgtt gaaactgaaa
      661 aattatcgtt tggcgcaaac ggcaagaaag tcaacatcat aagcgacacc aaaggcttga
      721 atttcgcgaa agaaacggct gggacgaacg gcgacaccac ggttcatctg aacggtatcg
      781 gttcgacttt gaccgatatg ctgctgaata ccggagcgac cacaaacgta accaacgaca
      841 acgttaccga tgacgagaaa aaacgtgcgg caagcgttaa agacgtatta aacgcaggct
      901 ggaacattaa aggcgttaaa cccggtacaa cagcttccga taacgttgat ttcgtccgca
      961 cttacgacac agtcgagttc ttgagcgcag atacgaaaac aacgactgtt aatgtggaaa
     1021 gcaaagacaa cggcaagaaa accgaagtta aaatcggtgc gaagacttct gttattaaag
     1081 aaaaagacgg taagttggtt actggtaaag gcaaaggcga gaatggttct tctacagacg
     1141 aaggcgaagg cttagtgact gcaaaagaag tgattgatgc agtaaacaag gctggttgga
     1201 gaatgaaaac aacaaccgct aatggtcaaa caggtcaagc tgacaagttt gaaaccgtta
     1261 catcaggcac aaatgtaacc tttgctagtg gtaaaggtac aactgcgact gtaagtaaag
     1321 atgatcaagg caacatcact gttatgtatg atgtaaatgt cggcgatgcc ctaaacgtca
     1381 atcagctgca aaacagcggt tggaatttgg attccaaagc ggttgcaggt tcttcgggca
     1441 aagtcatcag cggcaatgtt tcgccgagca agggaaagat ggatgaaacc gtcaacatta
     1501 atgccggcaa caacatcgag attacccgca acggtaaaaa tatcgacatc gccacttcga
     1561 tgaccccgca gttttccagc gtttcgctcg gcgcgggggc ggatgcgccc actttgagcg
     1621 tggatgacaa gggcgcgttg aatgtcggca gcaaggatgc caacaaaccc gtccgcatta
     1681 ccaatgtcgc cccgggcgtt aaagaggggg atgttacaaa cgtcgcacaa cttaaaggcg
     1741 tggcgcaaaa cttgaacaac cgcatcgaca atgtggacgg caacgcgcgt gcgggcatcg
     1801 cccaagcgat tgcaaccgca ggtctggttc aggcgtatct gcccggcaag agtatgatgg
     1861 cgatcggcgg cggcacttat cgcggcgaag ccggttacgc catcggctac tccagtattt
     1921 ccgacggcgg aaattggatt atcaaaggca cggcttccgg caattcgcgc ggccatttcg
     1981 gtgcttccgc atctgtcggt tatcagtggt aagggctttt tcgcctgtct gctgttggga
     2041 caggcggaag gtttgaaggg aagggtggcg atttgccgcc tgagaccttt gcaaaattcc
     2101 ccaaaatccc ctaaattccc accaagacat ttaggggatg ttccatgagc accttctttc
     2161 agcaaaccgc acaagccatg attgccaaac acatcgaccg tttcccgcta ttgaagttgg
     2221 atcaagtgat tgattggcag ccgatcgagc agtacctgaa ccgtcaaaaa aaccgttacc
     2281 tt
//
LOCUS       DAWFAN010000209         2268 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWFAN010000209 DAWFAN010000000
VERSION     DAWFAN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.209"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..2108
                     /locus_tag="WGO65_001914"
     CDS             <1..2108
                     /locus_tag="WGO65_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0328157.1"
                     /translation="CINANNPNKSTKAPSPALTAPALWFGPVQNGKMEMYSASVSTYP
                     DSSSSRIFLQNLKRKTDPNRPGRHSLETLNKAQIESREPNFTGRETVIRLDSGVHEIK
                     LKGNEVEGFKGNNGNDTFGIVKDLGVDPDASEWKKVLLPWTVRGSNDDGQFNTFNKEE
                     NNGKPKYSQKYRSRDNGKHERNLGDIVNSPIVAVGGYLATSANDGMVHIFKQSGGDKR
                     SYNLKLSYIPGTMPRKDIQSQDSTLAKELRAFAEKGYVGDRYGVDGGFVLRQVERNGK
                     DHVFMFGAMGFGGRGAYALDLTKAENGNPTAVSLFDVKHDNNGVQLGYTVGTPQIGKT
                     HNGKYAAFLASGYATKQIDHTDNKTALYVYDLESSGTLIKKIDVPGGKGGLSSPTLVD
                     KDLDGIVDIAYAGDRGGNMYRFDLSGQDPNQWSVRTIFSGNKPITSAPAISQLKDKRV
                     VIFGTGSDLSEEDVDNNDIQSIYGIFDNDTDTGTAQDGQGNGLLEQVLKKDGNTLFLS
                     DYKRSNGSGDKGWVVKLEAGQRVTVKPTVVLRTAFVTIHKYTGTDKCGAETAILGINT
                     ADGGKLTKKSARPIVPEANQAVAQYSGHKKGTNGKSIPIGCMWKNNETVCPNGYVYDK
                     PVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMNDLDSL
                     DITGPTCGMKRISWREIFY"
BASE COUNT          632 a          578 c          596 g          462 t
ORIGIN      
        1 gctgcatcaa cgcaaataac cccaataaga gtaccaaagc cccttccccc gcactgactg
       61 cccccgcgct gtggttcgga cctgtgcaaa atggtaagat ggagatgtat tccgcttcgg
      121 tttctaccta ccccgacagt tcgagcagcc gcatcttcct tcaaaatctg aaaagaaaaa
      181 ccgaccccaa cagacccggc cgccattccc tcgaaacctt gaataaggcg cagattgaaa
      241 gtcgagagcc gaatttcacg gggcgggaaa ccgtcatccg attggatagc ggcgtacatg
      301 agatcaaact taaaggcaat gaggtcgaag gttttaaggg aaacaacggc aacgacactt
      361 tcggcattgt taaggacttg ggcgtcgatc ctgatgccag cgagtggaaa aaagtattgc
      421 tgccttggac ggttcgaggt tccaatgatg acggtcaatt taatacattc aacaaagaag
      481 aaaacaacgg caagccaaaa tacagtcaaa aataccgcag ccgcgacaac ggcaagcacg
      541 agcgcaattt gggcgacatc gtcaacagcc ccatcgtggc ggtcggcggg tatttggcaa
      601 cttctgccaa cgacgggatg gtgcatatct tcaaacaaag cggcggggac aagcgcagct
      661 acaatctgaa gctcagttat atcccgggca cgatgccgcg caaggatatt caaagccaag
      721 actccaccct tgccaaagag ctgcgcgcct ttgccgaaaa aggctatgtg ggcgaccgct
      781 acggcgtgga cggcggcttt gtcttgcgcc aagtcgaacg gaatgggaaa gaccatgtgt
      841 ttatgttcgg cgcaatgggc tttggcggca gaggcgcata cgccttggat ttgaccaaag
      901 ccgaaaacgg taacccgacc gccgtttccc tgtttgatgt caaacatgac aataatggcg
      961 tgcaattagg ctacaccgtc ggcacgccgc aaatcggcaa aacccacaac ggcaaatacg
     1021 ccgccttcct cgcctccggt tatgcgacta aacaaattga ccacacagac aataaaaccg
     1081 cgctgtatgt gtatgatttg gaaagcagcg gtacgctgat taaaaaaatc gatgtacccg
     1141 gcggcaaggg cgggctttcg tcccccacgc tggtggataa agatttggac ggcattgtcg
     1201 atatcgccta tgccggcgat cgcggcggga atatgtaccg ctttgatttg agcggtcaag
     1261 atcctaatca atggtctgta cgcaccattt tcagcggcaa caaaccgatt acttccgcac
     1321 ccgccatttc ccaactgaaa gacaaacgcg tggttatctt cggtacgggc agtgatttga
     1381 gtgaggaaga tgttgataat aatgatatac aatctattta cggtattttt gacaatgaca
     1441 cagacacggg tacggcgcaa gacgggcagg gcaacgggct gctcgagcaa gtgctgaaaa
     1501 aagacggcaa tacgctgttc ttaagcgact acaaaaggtc taacggctcg ggcgataagg
     1561 gctgggtagt gaaattggaa gccggacagc gcgttaccgt caaaccgacc gtggtattgc
     1621 gtaccgcctt tgtaaccatc cataaatata cgggtacgga caaatgcggc gcggaaaccg
     1681 ccattttggg tatcaatacc gccgacggcg gcaagctgac caagaaaagc gcgcgcccga
     1741 ttgtgccgga agccaatcag gctgtcgcgc aatattccgg tcataagaaa ggcaccaacg
     1801 gcaaatccat ccctataggt tgtatgtgga aaaacaatga aaccgtctgc ccgaacggat
     1861 atgtttacga caaaccggtt aatgtgcgtt atctggatga aaagaaaaca gacggatttt
     1921 caacaacggc agacggcgat gcgggcggca gcggtataga ccccgccggc aagcgttccg
     1981 gcaaaaacaa ccgctgcttc tcccaaaaag gggtgcgcac cctgctgatg aacgatttgg
     2041 acagcttgga cattaccggc ccgacgtgcg gtatgaaacg aatcagctgg cgtgaaatct
     2101 tctactgatt tgcacgcgaa aatgccgtcc gaaaggtttt cggacggcat tttttgcgtt
     2161 tttcgggagg ggcgggttcg taaaaggcgg gctatagggt aggcttcatc tcgccaatct
     2221 cactgaatcc atcaatttcc acaattcaat taaataccgt caaaccga
//
LOCUS       DAWFAN010000210         2216 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWFAN010000210 DAWFAN010000000
VERSION     DAWFAN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.210"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            99..1910
                     /gene="typA"
                     /locus_tag="WGO65_001915"
     CDS             99..1910
                     /gene="typA"
                     /locus_tag="WGO65_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ0328158.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          564 a          631 c          579 g          442 t
ORIGIN      
        1 gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc gtggtataat
       61 gcgcagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg
      121 ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg ctgcgccaat
      181 ccggcacatt ccgcgccaac cagcaggttg acgagcgcgt gatggacagc aacgacctcg
      241 aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac gaaggctacc
      301 acatcaatat cgtcgacacg ccgggacacg ccgacttcgg cggcgaagta gagcgcgttt
      361 tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa
      421 cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc gtcatcaaca
      481 aaatcgacaa gccgtccgct cgtccgagct gggtcatcga ccaaactttc gagctgttcg
      541 acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttatgct tccggtctgt
      601 ccggtttcgc caaattggaa gaaaccgacg agagcaacga catgcgtccg ctgttcgata
      661 ctatcttaaa atatacgcct gcaccgagcg gcagcgcgga cgaaacgctg caactgcaaa
      721 tttcccaact cgactacgac aactacaccg gccgcctcgg tatcggtcgt atcttgaacg
      781 gacgaatcaa acccggccaa accgttgccg tcatgaacca cgatcagcaa atcgcccaag
      841 gccgcatcaa ccagcttttg ggtttcaaag gtttggaacg cgtgccgctt gaagaagccg
      901 aagccggcga catcgtgatt atttccggta tcgaagacat cggtatcggc gtaaccatca
      961 ccgacaaaga caatcccaaa ggcctaccga tgttgagcgt ggacgaaccg acgctgacga
     1021 tggactttat ggtcaacacc agcccgctgg cgggtacgga aggcaaattc gtaaccagcc
     1081 gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag
     1141 ataccgccga tgccgacgtg ttccgcgtat ccgggcgcgg cgagctgcac ctgaccattt
     1201 tgctggaaaa tatgcgccgc gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt
     1261 accgcgacat cgacggtcaa aaatgcgaac cgtatgaaaa cctgaccgtg gatgtacccg
     1321 acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa ctgactaata
     1381 tggaaagcga cggcaacgga cgcacccgcc tcgaatacca tattccagcg cgcggcttga
     1441 tcggtttcca aggcgaattt atgaccctga cgcgcggggt cgggctgatg agccatgtgt
     1501 tcgacgatta cgcgcccgtc aaacccgata tgcccggccg ccacaacggc gtgctggtgt
     1561 cccaagagca gggcgaggca gtcgcttacg ccttgtggaa tctggaagac cgcggtcgta
     1621 tgttcgtatc acccaacgac aaaatctacg aaggtatgat tatcggcatc cacagccgcg
     1681 acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc cgcgccagcg
     1741 gcaccgacga agccgtgcgc ctgaccacgc cgatcaagct gacgctggaa ggtgcggttg
     1801 agtttatcga cgatgacgaa ctcgttgaaa tcacgccgca atccatccgt ctgcgcaagc
     1861 gttacttgag cgaattggaa cgccgccgcc actttaaaaa gctggattga tgtttactgg
     1921 attaatgttt aaatgatacg cgatgccgtc tgaaaaattt cagacggcat tttttattcg
     1981 gacgggcttt gcggcttctt gaagctgttt cagacggcgt ttttcctacc caatcaagaa
     2041 actgccgcca tttttccagc ggtatatcgc cccgtcgcgt ttcggtatcg ggttcggctt
     2101 cccggcagca tgtcgaacaa tgccgtttga aggaatacgc cttttgagtc cttacaagct
     2161 taggaagagg atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcaga
//
LOCUS       DAWFAN010000211         2163 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWFAN010000211 DAWFAN010000000
VERSION     DAWFAN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.211"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            373..885
                     /locus_tag="WGO65_001916"
     CDS             373..885
                     /locus_tag="WGO65_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ0328159.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            965..2044
                     /gene="aroB"
                     /locus_tag="WGO65_001917"
     CDS             965..2044
                     /gene="aroB"
                     /locus_tag="WGO65_001917"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ0328160.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          529 a          676 c          526 g          432 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gccaaggcga ggcaacgctg tactggtttt
       61 tgttaatcca ctatagtatt gataaacata ttatcttcaa tatattcaat tggataattg
      121 tttacctaag caaagataat tgccttttcc tgacaaataa gtgaaatcaa cggattgtca
      181 aaacacagcc tgaaataaaa aacctccctg atttctttta tttgtcctta aaatcagaaa
      241 ggttcgggat ggtcgggtta tttttccaaa cgtaccgccg ccctgccgat ttcgtataaa
      301 attccgccgt aacccgacaa gcccgaaccc tgtcgccccg aaaggcgggg tgtcaaacat
      361 taaggaattg tgatgaaaaa ctttaacggc aaactcatcc tcatcggact gatgggcgcg
      421 ggcaaaacca cgctgggccg gcaaatggcg cagcggctgg attaccgttt ttacgacagc
      481 gatcacgaaa tcgccgtagc cgccggcgtt cccatcccca ccatatttga aatggaaggc
      541 gaacagggat tccgttcgcg cgaaaccgcc atactcaaaa agctggttat cctgccccat
      601 atcgtcctgt ccaccggcgg cggcgcggtg ttaaaagaag aaaaccgcgc ccttatccgc
      661 aaaagcggca cggtcgtcta tctgcacgcc ccgcccgaaa ccctgctcga acgcacgcgc
      721 tgcgacaaca gccgtccttt gctgcaagtt gccgatcctt tggcgaaatt acgtgaactc
      781 tacgccgcac gcgaccccgt ttaccgccaa accgccgact ttaccgtaga atcggcaaac
      841 tgccgggaaa ccgtgcaaac cctgctcaaa cgcttatccc gataaaccgg catatgcgcc
      901 gcgcccagaa aaccaagccg cgccccgccc ggcgggccgg cggttcaaac tttaaggaac
      961 aacaatgaaa acactgaccg tacacacccc gtcccacagc taccccatct ttatcggcaa
     1021 cggactgctg ccgcaggcag gaagcctgct caaaccgcat ttgggcaaac gcgccgccat
     1081 catcgccaac gaaaccgtcg ccccgctcta cctcggcacg cttcagacgg cattggatgc
     1141 ggcaggcgta tcccatttca gcatcatcct gcccgacggc gaggcgcaca aaaactggca
     1201 gacgctcaac ctcatctttg acgggctgat gcaaaaccgc gccgaacgca aaaccacatt
     1261 aatcgcactg ggcggcggcg tgatcggcga catggtcggc tttgccgctg ccacctacca
     1321 gcgcggcgca ccgttcgttc aaataccgac cacactgttg agtcaagtcg actcatcggt
     1381 gggcggcaaa accgccatca accacccgct cggcaaaaat atgatcggcg cattctacca
     1441 gccgcaggcg gtgcttgccg acttggacac gctgcacacc ctgcccgtcc gcgaattgtc
     1501 cgccggtatg gcggaagtca tcaaatacgg cgcgctcggc gacatcggtt ttttcgaatg
     1561 gctcgaacaa aacatatccg gactgatgac gctcgatcgg gaaaaactcg cccaagccgt
     1621 gtaccgctgc tgccaaatga aggcagacat cgtcgcccaa gacgaaaccg aacagggcat
     1681 acgcgcatgg ctcaacctcg gacacacctt cggacacgcc attgaaaccg agatgggtta
     1741 cggcacttgg ctgcatggag aagccatcgc cgccggctgc gtgttggcgg cgcgtttgtc
     1801 cgaacaactg ggcaaaacct ccgccgcaga taccgcgcgg ctcgccgccc tgctcgaagc
     1861 cgctggactg ccgtccgcac cgcccgtgtt tgcctttgaa aaatggctcg aacatatgag
     1921 ccacgataaa aaagtcagcg gcggcatcat gcgctttatc ggtctgaacc ggctgggcga
     1981 agccaacatc accgaaatta ccgacacgga catcctccgc cgcaccctgc aaccgtatct
     2041 ctgatttcct ctgccgatgt gctgccgcgc gggtttgacg cacgatgatg attttccatc
     2101 atctttctcc gtaaaagcgg gaatccggtc cgttcggttt cggtcgtttc cgataaattc
     2161 ctg
//
LOCUS       DAWFAN010000212         2126 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWFAN010000212 DAWFAN010000000
VERSION     DAWFAN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.212"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(127..555)
                     /locus_tag="WGO65_001918"
     CDS             complement(127..555)
                     /locus_tag="WGO65_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ0328161.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(536..970)
                     /locus_tag="WGO65_001919"
     CDS             complement(536..970)
                     /locus_tag="WGO65_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ0328162.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLCRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKASGDDGVRKS"
     gene            complement(1143..1559)
                     /locus_tag="WGO65_001920"
     CDS             complement(1143..1559)
                     /locus_tag="WGO65_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328163.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
     gene            complement(1556..1747)
                     /locus_tag="WGO65_001921"
     CDS             complement(1556..1747)
                     /locus_tag="WGO65_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328164.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
BASE COUNT          562 a          574 c          397 g          593 t
ORIGIN      
        1 aacatttgaa aaccccatta aaccgtcttt aacccatcgt cccgaacggt agatgtttca
       61 tcccctgcga accaaaaaaa agaccgtctg aatacagacg gccaaagcct ggtcacacat
      121 cactgcctaa aataaagctg cctgctgtgc cgcaggtcgg ctcatttcat tgataatcgt
      181 atatcccgtt cgtgaagaga tgccgtattt agggcatagc ttcgtcatcg ccataagccc
      241 gctctcctta tcaatatcgc gcaatttgac aaactcctga taaaacctat ggtttctcaa
      301 ctgtattaac gccttgccgc accgtgggac atacaattcc tcgccaccat atacctgcaa
      361 cagctcatgt gttttcactt cgccgatggc ttcgaccaaa attgccaaac gctcggtgtc
      421 caccttgccc ttaccaaatt taaaccgcgc cccgccaatc gccttgacca gctgttccgt
      481 cgctgccagt ccgatgacat ccacaatgtc caacacggta tccggcaata aatgttcaac
      541 tttttcgaac cccatcatcc ccactcgctt tttccgtttt cctgttttcc gcaatctgca
      601 acgcagcaac cagtttatgt agctgcgtat cgtctaaata ttcgacctta tccttaccaa
      661 acatccgccg cgccattgcg tgtgcatagt tccaatgttt gccgccgacg gtcagcaggg
      721 cttcgacttt gtccaacatt gccgctgatg atgtccgaca cagatgcggt ttgccgtgtg
      781 ggttaccttt tgctttaggc ttaaatccgt gcgaccgcat atcagcgaca acagactcaa
      841 gttcagaaac atccatatcc gcacacgacc gcttatccgt cacacgctcc aacaccgcgc
      901 gataggtacc gtcatccaag cccagctcct tttgagcaat cttaattttc gcaatcaacg
      961 cccggcgcat tatctctcca atacaacata tagtataaat tagcgcattt tatacaataa
     1021 aatacaatat atagtattaa gccgatgttt ttttgcgaaa cggacagaca taaaaaatgc
     1081 cgtctgaaaa cgagctttgc gagtttcgcc aaaacgtttc agacgacctt ttttactaca
     1141 atctaatcac tgtctttttc gcgccgttgc ttttccaagg tttgcgcttt acctctgttg
     1201 attcctgcaa aatatcccaa agctaccgcc ccgccgattt tgacaatttc aatagcgacg
     1261 ctgtccttat cgcgccataa cgcagtaatc agcacggcgg caatcagaaa agaaatagca
     1321 atcacggcat tcagacggcg tttgtgtacc tctttgaaaa tctcaccatg ttgctgttca
     1381 ccctgttttt gcgcttcgat ggtagccagt gcaatgcgtt cgtgagattg gatttcgtcc
     1441 ttacggcttt ccaactcctt ctgtttgact gcaatatcct gtttttgaag ttctaaaaac
     1501 tcctgaagca ttttctgctg ctcatcggcc ggcagattct cggatttttc cccgctcata
     1561 aatcaaccgc cactaaggtt ttgtaacgga ctttgaattt gccgtaaccc cgagccccta
     1621 ttttcggcgg cacaaattcc acggacgcaa tattgcccgt cttacggtct ttacggactt
     1681 cttccaaata atcggcagca ttgagcagcc gttcgcggac tgccgtatat tctttgacgg
     1741 tattcatttt tcaacctcgc ccaaatagtg caaatattag catatgggat ttatttttca
     1801 agaaaatcaa atccatataa aacaaccgta taatatttta atactatata tggtaaattt
     1861 cttctaaaaa tacacactaa aataccatat cttgataacc gcaacgccgc acaaatggca
     1921 gggcattgat gcccgacaac aaaaaatgcc gtctgaaaca gcattcgagc tgttttcaga
     1981 cggcattttt ctttgatttt gttggggaaa gggttgactt ttaacgtgtc cacgtttata
     2041 attcaattca tcggcgtagt gagggccgat acgggaaaag ccccaccgaa gcggggctca
     2101 actgggagaa agaagatgaa taaacg
//
LOCUS       DAWFAN010000213         2122 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWFAN010000213 DAWFAN010000000
VERSION     DAWFAN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.213"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            123..1508
                     /locus_tag="WGO65_001922"
     CDS             123..1508
                     /locus_tag="WGO65_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ0328165.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     DFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1528..2046
                     /locus_tag="WGO65_001923"
     CDS             1528..2046
                     /locus_tag="WGO65_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ0328166.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTKQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          448 a          646 c          570 g          458 t
ORIGIN      
        1 ttcaaataaa caggaatata acttcagaca aacaacttac cgcccgattt gtgctatcgt
       61 tttcgcacaa cttaaaaaaa cctgacaatt ttgtactttt attacagaga aaggctttac
      121 aaatggacgg ctggacacag acgctgtccg cgcaaaccct gttgggcatt tcggcggcgg
      181 caatcatcct cattctgatt ttaatcgtca aattccgcat ccacgcgctg ctgacactgg
      241 tcatcgtcag cctgctgacg gctttggcaa ccggtttgcc cacaggcagc attgtcaacg
      301 acgtactggt caaaaacttc ggcggcacgc tcggcggcgt ggcgcttctg gtcggcctgg
      361 gcgcgatgct cggacgtttg gtcgaaacat ccggcggcgc acagtcgctg gcggacgcgc
      421 tgatccggat gttcggcgaa aaacgcgcac cgttcgcgct gggcgttgcc tcgctgattt
      481 tcggcttccc gattttcttc gatgccggac taatcgtcat gctgcccatc gtgttcgcca
      541 ccgcacggcg catgaaacag gacgtactgc ccttcgcgct tgcctccatc ggcgcatttt
      601 ccgtcatgca cgtcttcctg ccgccccatc cgggcccgat tgccgcttcc gaattttacg
      661 gcgcgaacat cggccaagtt ttgattttgg gtctgccgac cgccttcatc acatggtatt
      721 tcagcggcta tatgctcggc aaagtgttgg ggcgcaccat ccatgttccc gttcccgaac
      781 tgctcagcgg cggcacgcaa gacaacgacc tgccgaaaga acctgccaaa gcaggaacgg
      841 tcgtcgccat catgctgatt cccatgctgc tgattttcct gaataccggc gtatcggccc
      901 tcatcagcga aaaactcgta agtgcggacg aaacctgggt tcagacggca aaaataatcg
      961 gttcgacacc gatcgccctt ctgatttccg tattggtcgc actgtttgtc ttgggacgca
     1021 aacgcggcga aagcggcagc gcgttggaaa aaaccgtgga cggcgcactc gcccccgtct
     1081 gttccgtgat tctgattacc ggcgcgggcg gtatgttcgg cggcgttttg cgcgcttccg
     1141 gcatcggcaa ggcactcgcc gacagcatgg cggatttggg cattcccgtc cttttgggct
     1201 gtttccttgt cgccttggca ctgcgtatcg cgcaaggttc ggcaaccgtc gccctgacca
     1261 ccgccgccgc gctgatggct cctgccgttg ccgccgccga ctttaccgac tggcagctcg
     1321 cctgtatcgt attggcaacg gcggcaggtt cggtcggttg cagccacttc aacgactccg
     1381 gcttctggct ggtcggccgc ctcttggaca tggacgtacc gaccacgctg aaaacctgga
     1441 cggtcaacca aaccctcatc gcactcatcg gctttgcctt gtccgcactg ctgttcgcca
     1501 tcgtctgaca gacggaaagg atagtaaatg actacgcatt ttgtcgttat gggcgtatgc
     1561 ggctgcggca agaccaccgc cgcgctgtcc ctgcagaaac acctcggtca atgtccctat
     1621 gccgaaggcg acgagttcca cacccaagcc aaccgcgaca agatgggcgc gggtattccg
     1681 ctgaccgatg aagaccgcta tccgtggttg ggcaatctgc gcgactggat gacgaaacag
     1741 gcgcaaaacg gtgcgaacca caccatcgta acctgttccg ccctcaaacg cggctaccgc
     1801 gacattctgc gcggagccga aggcaaagct gccttcatcc acctcagtcc gccgcaagac
     1861 atcaacctcg agcgcatgat gtcgcgcaaa ggacattaca tgaaagcagg gatgctcgat
     1921 tcgcaactgg aaatcctcga ggaactgggc gaaggcgaat acggagtcaa aatcgccaac
     1981 cccggcacgc ccgaagcggt cgaagccgat attctgaact gggttgcctc ggaaaacctg
     2041 ctttgaagca atatgccgtc tgaagcccga cacaggatgg gtttcagacg gcataaacat
     2101 cgggaacaga atggattaca tt
//
LOCUS       DAWFAN010000214         2007 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWFAN010000214 DAWFAN010000000
VERSION     DAWFAN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.214"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            307..1764
                     /locus_tag="WGO65_001924"
     CDS             307..1764
                     /locus_tag="WGO65_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0328167.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPIVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          456 a          590 c          459 g          502 t
ORIGIN      
        1 ctataatgcg cccttcggcg tggcggatat ataaggaagt gattttccat ctaagtaaaa
       61 accgccctat cggataagcc cttaacagaa aaggctttac ccgcgccgta tcggaacaca
      121 tcctctaaaa tacaatccgt tgaattgaaa aaaatataaa aacatccgcc cgcgaaaaac
      181 ggcagcgcgt cgtttgacaa agaatgaaaa tatcggttaa aaaccgatat tttcatacaa
      241 aaaacaccgc tgccgtccgc atccgtttca gacggtattg agagaaaatc ttttaggaga
      301 acctttatgt cccggcatcc cgcccccacc ggagaaaaaa cattcttcgg ccaccccttc
      361 cagctttcca ccctcttcca tatcgaattg tgggaacgtt tttcatttta cggaatgcag
      421 ggcatcctgc tgatttacct ctactacacc gccgacaaag gcggcttggg catagacaaa
      481 accctcgccg gcggcattgt cggcgcatac agcggcagcg tgtacctgtc caccattttg
      541 ggggcgtggt ttgccgaccg agtatggggt gcggaaaaaa ccctcttcct ctcgggcatc
      601 gtcgtgatgc tcggacacat cgtccttgcc gccgccccgg gcctgtacgg ccttttaatc
      661 gggctgatat tcatcgcatt gggcagcggc ggtgtgaaat ctacggccag ctctatggta
      721 ggcgcattat acgaacagga cgaaatgcgc ccgctgcgcg atgcgggatt ttccattttc
      781 tacatcgcca tcaatatcgg cggcttctta ggcccgctgc tgaccggcct gctgcaggaa
      841 aacatcggtt tccattatgg tttcggcgcg gcggcggtcg gtatggcatt cggcttgtgg
      901 cgttattcct tgggacgtaa aaacctgccc catcccatcg tcccccatcc gctttcaaaa
      961 ggacagggca aaactgcggc cgccgtcggc attgctctca tcgccgcact tgcaaccgcc
     1021 atcaaaaccg ggcttgtcaa cctcgacaat ttctccggca tcctcttatc taccgtcatc
     1081 cttgccgtca ttgcctattt tgcccgcctg ctgactaatc cccgcgtcag ttccgacaac
     1141 aaacggcaca tcatcgccta catcccgctt ttcctgacca tctgtatgtt ttgggccgtc
     1201 tggtttcaga tttacaccgt agcaaccgtc tatttcgacg aaaccgtcaa ccgcaccatc
     1261 ggttcgttta ccgtgcccgt cgcctggaaa gattccatgc aaagcctgtg ggtcatcctg
     1321 ttttccggac tgatggcggc aatgtggaca aaaatggggc gcaaacagcc caaaaccccg
     1381 ctgaaattcg ctatggcggt atttgttacc ggcgcgtcgt ttttgggatt cgtccccttt
     1441 atttccgccg gcacgccgat gcctattgcg gttttcgcac tgattgtcct tgccatcacg
     1501 ataggcgaac tgatgatttc cccgattgcg ctgtccatct ccaccaaaat cgcaccgcct
     1561 ttattcaaaa cccaaatggt cgcccttaat ttccttgcct tttcattagg cttcactttg
     1621 ggcggcgtat tgtttgaaaa aggctatcag gcgggcgacg aaatcggctt ctatcggctg
     1681 ctgttctaca tcggcgcagc cacaggcttc ctgctgctcc tgctcgtccc caaattgaac
     1741 aaaatgctcg aaggcacaga ctaagtcccg ccccgatgcc gtctgaaccc ttcagacggc
     1801 atttttccgc ataatgaaac caaaccgttt ccacccgaca ggacaggctc cccccaaccg
     1861 gaaggcagcc tgccgattgt catttaaata tctgactgat tccgtctgtt tgcaagggaa
     1921 atagccgttt atttccgctt gggcggaaac cggttctatt gagtaaaagg cattttatcc
     1981 gactaaatta gtgctgcatc aacggaa
//
LOCUS       DAWFAN010000215         1938 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWFAN010000215 DAWFAN010000000
VERSION     DAWFAN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.215"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(4..1260)
                     /locus_tag="WGO65_001925"
     CDS             complement(4..1260)
                     /locus_tag="WGO65_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ0328168.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLQNLKIDL
                     DTGKYLRLDSAKHQTDGFFAAVLQRK"
     gene            complement(1608..>1938)
                     /locus_tag="WGO65_001926"
     CDS             complement(1608..>1938)
                     /locus_tag="WGO65_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328169.1"
                     /translation="VKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAYCLSM
                     LPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFTLAKE
                     IGKQKNI"
BASE COUNT          456 a          415 c          478 g          589 t
ORIGIN      
        1 ggtttatttg cgttgcaata cggcggcgaa gaagccgtcg gtttggtgct ttgccgaatc
       61 gaggcgcagg tattttccgg tatccaaatc aatcttcagg ttttgcagca gttcggcgca
      121 gttgacgggt tcaaattcgg gatgttcgga caggaaacgt tcgacttgca gctcgttttc
      181 ttcgggcagg atgctgcaag tggcgtaaac caagcggcct tgcggtttta ccagtttgga
      241 ggcagcatcg aggatgctgt gttgctgttc caaaaggttg gccacggtct cggcagattg
      301 gcggtatttg aggtcgggat tgcggcgtaa agtacccaaa ccggagcagg gcgcgtccac
      361 caacacacgg tcggctttgc ctgccagtcg ggcgatacgg ctgtcgtgtt cgctgctgat
      421 gcgttcgggg tggatattgg tcagtccggc gcgggtcata cgcggtttga ggttggcaag
      481 gcgtttttcg gcgatgtcga aggcgtagat tctgccttta ttggccattt gcgcaccgac
      541 agccaaggtt ttgccgccgg caccggcaca gaaatcgaca atgatttcgc cgcgttttgc
      601 gcccaccaat aaagcaagca gctggctgcc ttcgtcttga acttccagcg taccgtccaa
      661 aaacagctcg tgtttgttaa gcgcgatttt gtttttcagg cggatgcccc aaggcgaata
      721 aggcgttgcc tctgcatcgg cactttcggc ttgcaacaat ggcagcactt tatcgcgttt
      781 gcctttcaag gtattgacgc gtatatcgag cggcgcagct tggttgatgc tgcggccgaa
      841 ggcgaggatt tcttcttcgc gccaatgctg ctgcagctgt tctaccagcc attgcggcaa
      901 ttctgcggcg gtattcaggc cgtctgaaaa ctcggtttta cgggctttca aattgccgag
      961 aaactctatt tcttcttcat caagcaggtc tttgatttgg ctgatgttgg tgcttctgcc
     1021 gagaaccagt gcggcgagtg cggctttgcg cggctgcgcg tgcggacggc gcagggcggt
     1081 actgattttt tgatagtggc gcagcgcggc aaaggcggtt tcggcgattt cgtggcggtc
     1141 ttggcggccg agctttttgt gttcgcggaa ataggcggag aggacggcat cggaaggctg
     1201 tttgaaagtc agcatttcag ccaaaacttt ggcggtatgg tcgagttgtg cggcgttcat
     1261 aatgtaatgg gttctcttac tgtgggttat tcataatcaa tctaacgtcc ttaaaataac
     1321 aatagcatct ttatcattac ttagtattct atccgcaata agaaagatgt gttaaaagtg
     1381 cgcaatcggg atttgtctaa atttctccgt taactacaaa ggcagcctga aaaaagttct
     1441 attttcaggt cgcttgaaca gcatccgata taatcacatc agcgatttca ttttgtaatc
     1501 actccctaaa acatgaggaa aaaaacaaaa tttattttct tcgttcagta tatctatcct
     1561 aaatatgcca tcactccatt gagtttttaa tttgtatttc gctcttgtta aatgtttttc
     1621 tgtttgccga tttcttttgc taatgtaaaa aatactctac cactctcatt ggttaaagtt
     1681 ttcaagtatt catcaacatc agtaacatta ataacaactg tttcatttgt atgtttatta
     1741 aattctaaat caataataat attcccatta accaatattt catatatatc ttcttcttca
     1801 gggagcatag acaaacaata agcacttgaa gctgaaaact cttttttcag atactgaaga
     1861 agaaaagtat ttttaattaa aatattatta aatttatcta atgattgctt gacttcttta
     1921 gttgtccaat ttttaaca
//
LOCUS       DAWFAN010000216         1893 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWFAN010000216 DAWFAN010000000
VERSION     DAWFAN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.216"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            237..1196
                     /locus_tag="WGO65_001927"
     CDS             237..1196
                     /locus_tag="WGO65_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328170.1"
                     /translation="MYQNYDIVILSLGNGLLTRFFRNSSKLNLTLPIVITLFPGVIFG
                     DSESIITRLGANILLFNNYYDYQIGNKFKERYNLSCTNILYGYPILRKKHHCSDGNKV
                     YFIDQVKIPDGKKERLLILKKLIALANRYPHKDLVLLLRVHQNDITVHEDKYSYAELA
                     KNMKLPDNFKIERKRTQDALQEMGYCLSFSSTMLFEAESCGIPIGVISDLGVDKKYAN
                     THFKDSGVLVNLDTVDLDSPNTINPTWAELYTNPDSLSSKEFNQLLEVTSTQPPSFDN
                     YFIAVNDVQPVSMVLLRKFKKLIYHPKQFFVDSKLYKLIKFTK"
     gene            1244..>1893
                     /locus_tag="WGO65_001928"
     CDS             1244..>1893
                     /locus_tag="WGO65_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEZ0328171.1"
                     /translation="MQKIRKAVFPVAGMGTRFLPATKASAKEMLPIVDKPLIQYAAEE
                     AVAAGCTELIFITGRNKRNIEDHFDKAYELETELEMREKHKLLGYVQKILPDHVSCIF
                     IRQAEALGLGHAVLCARPAVGNEPFAVILADDLIDAPQGALKQMVDIYNQSGNSILGV
                     ETVDPSQTGSYGIVEVEQLKSYQRVTNIVEKPKPEDAPSNLAVVGRYILTPRIFDLL"
BASE COUNT          636 a          310 c          334 g          613 t
ORIGIN      
        1 acttaatatc taatatttaa aatacaatga ataaaaaacg agtcctatta attgctagtt
       61 atgattcttt tttgaatact ggatatgctg ttgcaaaaga aattaactat tcaacaatcg
      121 atatttatat ccaccaaaca aaaaaaaata ttctttcgga atcacaatta agtttaaatg
      181 ggctttcttt aaaagaaatc aatttttttc atattgatga ttatttcgac aaattaatgt
      241 accagaatta tgatattgtt attctatcat taggtaatgg tttactgacg cgtttttttc
      301 ggaatagtag caaacttaat ttaacattac ctatagtaat tactctattt ccaggggtaa
      361 tttttggtga ttcagaaagt attataacta ggttaggtgc taatatttta ttgttcaata
      421 attattatga ttaccaaata ggcaataagt tcaaagaacg ttacaactta tcgtgtacaa
      481 atattttata tggttatcct atcctacgaa aaaaacacca ttgctctgat ggcaataaag
      541 tttatttcat tgatcaagta aaaattccag atggaaaaaa agaaagatta ttaattctaa
      601 aaaaattaat agcactggct aatcgctatc cacataaaga tttagtacta ttattacgtg
      661 tccatcaaaa tgatatcaca gtacatgaag ataaatactc ttatgctgaa ttagcaaaaa
      721 atatgaaatt gcctgataat tttaaaatag agagaaaacg gacacaagat gccttacaag
      781 aaatgggata ttgtttatca ttctcttcaa caatgttatt tgaagctgaa agctgcggaa
      841 ttcctattgg tgtcattagt gatttagggg ttgacaagaa atatgccaat acacatttca
      901 aagatagtgg tgttttggtt aatttagata cggtagactt ggactctcca aataccatta
      961 atccaacttg ggctgaacta tatactaatc cagatagcct ctcttcaaaa gagtttaacc
     1021 aattattaga ggttacttct actcaaccac ctagttttga taattatttt attgcagtta
     1081 atgacgttca gcctgtatct atggttctgt tacgtaaatt taaaaagctt atatatcatc
     1141 ctaagcaatt ctttgttgat tcaaaactat ataaattaat taaatttact aaataatttt
     1201 tactatgggt tgccttattt atttcaaagg agtgacataa aaaatgcaaa aaattcgtaa
     1261 agctgtattc cctgtagctg gcatgggaac ccgattctta cctgccacga aagccagcgc
     1321 taaagaaatg ctgccaatcg tggacaaacc gttaattcaa tatgctgcgg aagaagccgt
     1381 tgccgcgggt tgtactgaat tgatttttat caccggccgt aataagcgta atattgaaga
     1441 ccacttcgat aaagcctatg agttggaaac tgaactggaa atgcgtgaaa agcataaatt
     1501 gcttggctat gtgcaaaaaa ttctgcctga tcatgtcagc tgtattttca ttcgtcaggc
     1561 agaagctttg ggcttgggtc atgcggtatt atgcgcccgt cctgctgtcg gtaatgagcc
     1621 ttttgccgtg attttggccg atgacttgat tgatgcacca caaggcgcac tcaagcagat
     1681 ggtagatatt tacaatcaaa gcggcaacag cattttaggg gtagaaacag tcgatccttc
     1741 gcaaaccggt tcatacggca ttgtcgaagt ggaacagctg aaaagctatc agcgtgtaac
     1801 caatattgtg gaaaaaccaa agcctgaaga tgcgccgtca aatttagcgg tagtcggccg
     1861 ttatattttg acaccgcgta tttttgattt gct
//
LOCUS       DAWFAN010000217         1857 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWFAN010000217 DAWFAN010000000
VERSION     DAWFAN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.217"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..183
                     /locus_tag="WGO65_001929"
     CDS             <1..183
                     /locus_tag="WGO65_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEZ0328172.1"
                     /translation="AKAKAIAEYRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGA
                     PAPKGPAKPAIPAAKK"
     gene            271..720
                     /locus_tag="WGO65_001930"
     CDS             271..720
                     /locus_tag="WGO65_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0328173.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
     gene            complement(810..965)
                     /locus_tag="WGO65_001931"
     CDS             complement(810..965)
                     /locus_tag="WGO65_001931"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ0328174.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(1262..1474)
                     /locus_tag="WGO65_001932"
     CDS             complement(1262..1474)
                     /locus_tag="WGO65_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328175.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            1706..>1857
                     /locus_tag="WGO65_001933"
     CDS             1706..>1857
                     /locus_tag="WGO65_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0328176.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRG"
BASE COUNT          534 a          337 c          444 g          542 t
ORIGIN      
        1 gcgaaggcga aggccattgc ggaataccgt gcgcaaaacg gtgcgttcaa gtctgtagac
       61 gatttgacca aggtaaaggg catcggccct gcggtgctgg cgaagctgaa ggatcaggct
      121 tccgtcggcg cgcccgcacc aaaaggccca gccaaaccgg ctataccggc cgctaaaaaa
      181 tagagtggga aatatgcata ctgttgaatg ggatagtaag tctgttcaaa gaaatatgtt
      241 gaataatctg ttcttattgg aagtaaagta atgactgata atcgggggtt tacgctggtt
      301 gaattaatat cagtggtctt gatattgtct gtacttgctt taattgttta tccgagctat
      361 cgcaattatg ttgagaaagc aaagataaat gcagtgcggg cagccttgtt agaaaatgca
      421 cattttatgg aaaagtttta tctgcagaat gggagattta aacaaacatc taccaaatgg
      481 ccaagtttgc cgattaaaga ggcagaaggc ttttgtatcc gtttgaatgg aatcgcgcgc
      541 ggggccttag acagtaaatt catgttgaag gcggtagcca tagataaaga taaaaatcct
      601 tttattatta agatgaatga aaatctagta acctttattt gcaagaagtc cgccagttcg
      661 tgtagtgacg ggctggatta ttttaaagga aatgataagg actgcaagtt acttaagtag
      721 gcttgtttac aaatcgatcg atagtgtttc agtatttgtg cggttttccg ccttgatgga
      781 atgaaaatcg tgtaatatta agcctatagt tatgactcgt aatctgtatc tggattgttt
      841 ctggataaaa gaataaaccc aatcgttggg tcgtttgggt ttatttcatt gattgacaga
      901 agttttttaa taaatggaaa gacaacgcct tttttagggg atggttttgt ttttctctct
      961 gatattgcct ataggtttct gccccttcca ttctaaatat attatccgat tcggataagt
     1021 cgaatagggc acttgatgct aagccgatta caagtcgttt cgataaatca taggacataa
     1081 taaggatatc tttagccgtg gatttatggg gtatagtagc aaaaattgta taggggtgta
     1141 cattactcat ctgaaaaggt gaacagattt tataatgctt tcagacggca ttttgtttcc
     1201 aattcgttat aggcttgtat tttgcctatg taatatagta atttaaggta tgtttgcacc
     1261 tttagacata agggcaatcg cagctttcgt ctttaatata caaatcacta tccagcttca
     1321 gccataccca atagttttcc aaaccgcata cctccgcccg ataacgcccg gtggaagttt
     1381 tttggacagc accaactttg ccctgctgaa aatagagcag tccgcgttcg gcgatatggg
     1441 gcgggaacag gtggagcggg tggatatggg gcatggtttg cttccaagaa gttgttgaaa
     1501 tatgagaagt ttactggttc gggcgatgtt tggggagagg atggtgggaa gatttgtttt
     1561 acaaagtggc aaaaggtcgt ctgaaaatag ggttcagacg accttttggt ttagttggga
     1621 agggctgtgg tttaaaaaga ctgagacctt tgcaaaactc cccaaaatcc cctaaattcc
     1681 caccaagaca tttaggagat ttctcatgag caccttcttt cagcaaaccg cccaagccat
     1741 gattgccaaa cacatcgacc gtttcccact attgaagttg gaccaggtga ttgattggca
     1801 accgatcgaa caatacctga accgtcaaaa aacccgttac ctccgagacc accgcgg
//
LOCUS       DAWFAN010000218         1835 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWFAN010000218 DAWFAN010000000
VERSION     DAWFAN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.218"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            31..243
                     /locus_tag="WGO65_001934"
     CDS             31..243
                     /locus_tag="WGO65_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease III domain protein"
                     /protein_id="HEZ0328177.1"
                     /translation="MELETVVPPEEGEGEREGADGEMPEYLELHGGREDDISAPSQPE
                     PPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            278..1789
                     /gene="ubiB"
                     /locus_tag="WGO65_001935"
     CDS             278..1789
                     /gene="ubiB"
                     /locus_tag="WGO65_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ0328178.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT          403 a          499 c          494 g          439 t
ORIGIN      
        1 cttgtcctga tttttgttaa tccactacat atggagttag aaacggtcgt gccgccggag
       61 gaaggtgagg gcgaaagaga gggcgcggat ggggaaatgc ccgaatatct tgaacttcat
      121 ggcgggcggg aagatgatat ttccgcacct tcgcaacccg aaccgccgac ccgcaatatc
      181 aaaccgctgc ttttcgggct gctgattgcc tttttgggct atctgctcgg taagattttt
      241 tgattgtccg ataaatgctg tattcgggat tttatatatg aaatggttga aacgcctgac
      301 ggtcattgtc gggacttttt accgctatcg gctggcaggt ctgtgtgctt cgctgatggg
      361 tagcggttgg atatgcgctc tgctgaaaat gatgccgcag tcgtccaaat tgaaaaacga
      421 accgcctgcc gtccgtctgc gccttgcctt ggaaagcctg gggccgattt tcatcaagtt
      481 cgggcaggtt ttgtccacac gccccgattt gattccgcac gattacgccg tcgaactggc
      541 aaagctgcaa gacaaagtgc cgccttttga cgcgcggctt tcgcgtgaac aaatcgaaaa
      601 atcgttgggt cagtccatcg aaaagctgta tgcggaattt gaaaccgagc ccatcgccag
      661 cgcgtccatc gcccaagtac acaaagcccg cctgcattcg ggcgaacgag tggcggttaa
      721 agttttgcgc cccaaccttt tgccccttat cgaacaggat ttgtcgctga tgcgtttcgg
      781 cgcggcctgg gtcgaacgct tgttttcaga cggcaggcgt ctgaagccgc tcgaagtggt
      841 ggcggagttc gacaaatacc tgcacgacga gttggatttg atgcgcgaag ccgccaatgc
      901 cggacagctc gggcgcaatt tccaaaacag caatatgctg attgtgccca aggtgtttta
      961 cgactactgc accagcgacg tgctgacaat cgaatggatg gacggcacgc cggttgccga
     1021 catcgccaaa ctcaaagcag acggcatcga tttgcacaaa ctcgccgatt acggcgtgga
     1081 aatctttttc acgcaggtct tccgcgacgg ctttttccac gcggatatgc accccggcaa
     1141 tattttggtt gccgccgaca accgctacat cgccctcgat ttcggcatcg tcggcacgct
     1201 gaccgattac gacaaacgtt atctcgccat caatttcctc gcctttttca accgcgatta
     1261 ccggcgcgtc gccaccgccc acatcgaatc gggctgggtg cctgccgaca cgcgtgcgga
     1321 agaattggaa gccgccgtcc gcgccgtgtg cgaaccggtg ttcaacaaac cgatttcgca
     1381 gatttccttc ggcttggtgc tgatgcgcct gtttgaagtc agccgccgct tcaatgtcga
     1441 aatccaaccg cagcttgtgc tgctgcaaaa aacgctgctc aacatcgaag gcttgggccg
     1501 ccagctcgat cccgatttgg acttgtggaa aaccgccaaa ccgtttttgg tgaaatggat
     1561 gaacgggcag gtcggcccta aagccctttg gcgcaacctc aaaaacgaag cccccgactg
     1621 ggcgcaaatc atcccttcat tgccgcgcaa aatcagtgcg ttgattgatg aaaaccgcca
     1681 gcaggaaatg cgtgatgcct atatccattt ggtcaaagtg cagcagcggc aaagcctgtg
     1741 gctggctgtg attgcggttg ttttgctgct gattttgctt ttgaaatagg ctttgtccga
     1801 atcatcgccc gactccgccc gtttataagg aaatc
//
LOCUS       DAWFAN010000219         1828 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWFAN010000219 DAWFAN010000000
VERSION     DAWFAN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.219"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            324..1769
                     /gene="zwf"
                     /locus_tag="WGO65_001936"
     CDS             324..1769
                     /gene="zwf"
                     /locus_tag="WGO65_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ0328179.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT          500 a          423 c          484 g          421 t
ORIGIN      
        1 aaggtatacc gtctgaaagt gggaagcggg tgttattata gtacaaaatt tgtaattttg
       61 ttacaaaaaa atcagacatc gaaaagaaat ttgatttaaa tcgcagaatc tttcgattgt
      121 tgtaacggaa taggggaaac ctgcccgatt tttggactag gtcaaataaa ttgtaaaaat
      181 tgggaataga caaaaagtgt agcggtgtgt agtattacta ctcattatgc cgcctgcctt
      241 tgtttccgat attgcgatgc agcgtgatgg gggaaacagg tttcagacgg catcgaaaaa
      301 ttctttcctg acgagataat acgatgagta cacaaacaaa ttttgatttg gtgttgttcg
      361 gtgcgaccgg cgatttggcg atgcgcaagc tgttgccctg tctgtaccag gcgcatgttg
      421 ccggtctgct tcatcccgaa gggcgtattt tgggggtcag ccgcagcgag ttggataccg
      481 aaggttttct ggcgaaggta gaaaccagtt ccaaaatcca tgtgaaagaa aatttttccg
      541 atgaggcttg ggcatcgttt gtcgaacgtt tcgcatatct caaagtcgat gttacgcagc
      601 cggacgattt tgccgctttg ggcgatttgg tcaaagcgcg caaagaaacc gacaatgtcg
      661 tcatctatct ttccaccgcg ccgaaattct tcgcgcaaac atgtgaaaac cttgccaaaa
      721 tcggtttgaa cgccgacaac gtgcgtgtgg ttttggaaaa accgttgggt acggatttgg
      781 cttcttccca gcaaatcaat accgatgttg cgcgttattt caaagaaggg caaatttacc
      841 gcatcgacca ctaccttggc aaagaaagcc tgcaaaacct gcttgcgctg cgttttgcca
      901 atgtaatgtt cgagccgctg tggaacaata aatacatcga aagcgtgcag ttgaccatcg
      961 ccgaacagct cggtgtggaa gagcgcggcg agttttacga cattactggc gccttgcgcg
     1021 atatggtgca aaaccacttg atgcaaatgc tgtgcatgac tgcgatggaa gcccccgcca
     1081 gcttggatgc cgacgcggtg cgcgatgaaa aagtcaaagt catcaagtca ttgaagccgc
     1141 tgaccgtcga atctgtcaat gaaaatgtcg tgcgcggaca atataccgcc gccaaaggca
     1201 tgaacggcta tcttgaagaa atcaacgttc cgcaagacag ctttaccgaa acctacgtcg
     1261 ccattaaagc cgaaatcgaa aacgaacgct ggaagggcgt tcccttctac ctgcgtaccg
     1321 gcaagcgcat ggcgggcaaa gtggcggaaa tcgttttgaa cttcaaagat ttgaacagcc
     1381 atatttttga aggcagccgc accgcgccca accggctcgt tatcgagttg caaccatatg
     1441 aatccgtgcg cctctatacg cagatgaaaa ccccgggggc aggaaataag gtcgaaaccg
     1501 tgccgctggc aaccgatttg ggcaaagcat tggaaagccg ccgcgcggaa gcttacgagc
     1561 gcctgctgct ggatgtgatt aacggcaaac tcgctttgtt caaccgccgc gacgaacttg
     1621 aagccgcgtg ggaatatgtg atgccgattt tggaaaactg gacaaataac accacgccgc
     1681 cgcacggcta cggcgcacac tcgtgggggc ctgaagccgc gcgcgaacta ttggcgcgcg
     1741 acggacacaa gtggcacgaa gagcagtaat acaataatgc gttcagacgg catggggttt
     1801 gaaatgccgt ctgaacgcaa gtaaagca
//
LOCUS       DAWFAN010000220         1784 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWFAN010000220 DAWFAN010000000
VERSION     DAWFAN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.220"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            217..1689
                     /gene="pyk"
                     /locus_tag="WGO65_001937"
     CDS             217..1689
                     /gene="pyk"
                     /locus_tag="WGO65_001937"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0328180.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVTAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          459 a          519 c          483 g          323 t
ORIGIN      
        1 cagctccaaa aataaattta gcctaagtca taaaaccgtg taattttgca ccaaaaaaga
       61 cggtttgcag gtaaaatatc aaactattaa tttaaatcat atttttacag aatattccgc
      121 cgcattcatc aaatggacat caaaccggtt ccaaatttct gtaattttgc aacaaaatac
      181 cgcaaaacac ccgattgaga ccgaaaggac tttcatatga accagacaag ccgcgatctg
      241 acccgcatca gccacaacac caaaatcgtc gccaccttgg ggccgggcag caacaacgtc
      301 gaactgttgg aagacatgat ccgcgtcggc ggtctgaacg tcgtccgctt caacttcagc
      361 cacggcacgc ccgaattcca tcaggaaaac gcccgcattg tgcgcgaggc ggcaaaacgc
      421 gccggacagg aaatcgccat cattgccgac ctgcaagggc cgaaaatccg cgtgggcaaa
      481 atcgccggcg gcggcatcga attgaacaaa ggcgaaacgc tggtactgga tgccgcgctc
      541 gaaggcgaag gcacgcgcga ggcggtcggt ttggactacc gcgacctgcc cgacgacgtt
      601 accgcaggcg acgtattgtg gctggacgac ggcctgctga ccctgaccgt ggaatccgtc
      661 gaaggcagca ggattatcac aagggtggaa aacagccacg tcctgaaaag caacaagggc
      721 atcaacaaac gcggcggcgg tctatctgca ggcgcgttga ccgaaaaaga cttccgcgac
      781 ctgaaaaccg cgattgccat cggttgcgac tacctcgcca tcagctttgt gaaatccgcc
      841 gaagatttgc atatcgcgcg cgccaaagtc gaagaagaaa tgaagggcag cactgccgtg
      901 cgccccggtt tggtttccaa aatcgaacgc gtagaagcga ttgaaaacct tgatgaaatc
      961 atcctcgccg gcgacggcat tatggttgcg cgcggcgact tggcggtcga agtcggacac
     1021 gccgccgtcc ccgccctgca aaaacggatg atccgccgcg cccgcgagtt gcgccgcttc
     1081 agcattacgg cgacgcaaat gatggaatcg atgattacca accccgtgcc gacccgcgcg
     1141 gaagtcagcg atgtggcaaa cgcggtattg gacggcaccg atgcggtgat gtgttccgcc
     1201 gaaaccgccg tcggcgcgta tccgtttgaa accgtcagcc aaatggcgat tatctgcgcg
     1261 gctgcggaaa aagagcagga ttcgctcaac ggcgttgccg aacaggtcga gtatcccgaa
     1321 gcggtcagca ccaacctggc ggttgccggc ggtgcggtca gcgtggcgcg cgcggttcac
     1381 gccaaagcca tcgtcgccct gaccgaaagc ggttcgaccg cctttgaaat cagccgccac
     1441 aacatcaccc tgccgatttt cgcgctgacc ccgagcgttt ccgcccaacg ccgtatggcg
     1501 atgtaccggg gcgtgcgccc gctgattttg gcaaccagca ccgaccacga cacggcgtta
     1561 aacgaagtcg aaaccatgct ggtggaacac aacatcctgc attccggcga ccaatacatc
     1621 attaccagcg gctcgcaaat gcgcgaatcc ggttcgacca atacgctgga agtgctgcgc
     1681 gtcaaataat ccgccctgag tggaaaatgc cgtctgaagt cgatgcccga ggcttcagac
     1741 ggcattgttt taggcgcgat ttatagtgga ttaacaaaaa tcag
//
LOCUS       DAWFAN010000221         1752 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWFAN010000221 DAWFAN010000000
VERSION     DAWFAN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.221"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(110..766)
                     /locus_tag="WGO65_001938"
     CDS             complement(110..766)
                     /locus_tag="WGO65_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ0328181.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALVAAAILLLS
                     "
     gene            831..1721
                     /locus_tag="WGO65_001939"
     CDS             831..1721
                     /locus_tag="WGO65_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ0328182.1"
                     /translation="MFPVFHLSGESRRRMLQAASRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQETLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWMAGCGLGTDKAAVGTLAGILAEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT          396 a          419 c          579 g          358 t
ORIGIN      
        1 tgggacggaa aaacggcctg aagccgtttc gggcattcag accgtttgcg cggagagggg
       61 atgccgtccg aagggcgaaa agggcttcag acggcattga tgtcgggttt caggacagga
      121 gcaggatggc ggctgcgaca agcgcggcaa ccgataatgc ggcggcaagc gcggttttgc
      181 cggcaaagcg gattgaggtt ttgccttcga tgtatttgaa gccggttatc atcgggagga
      241 cgaggttttt ctttttgaac acgcggtatg cggcgacggc ggcgatatga atggcggaaa
      301 aaacggcgag cagcttgaaa aagttgaggt ggattttccg cataaggctg cccgtatgct
      361 cggaaaccaa atggttgagg tagccgttgg tgctgaaggt gttttcatcg gcggcaaaaa
      421 gccctgtgcc gacttgaaat gagacggcgg ccaaaagcgc gacgaccatc agtgcgccca
      481 aggggttgtg tccgggttgg acgtgttcgg gaataccgtt tttcatatac tctctgatac
      541 ccgaccatcc gcggacgaaa cgggagaaac gggcggtatc gctgccccaa atgccccagc
      601 agaggcggaa tacgagcagg aaaaggataa acagcccgac gcgcgtgtgc cattgcagca
      661 tatcgccgcc ggttttcgcg ctataccaca taaagggtag ggatgcggca agcagccagt
      721 ggaaaaggcg ggtggggaag tcccagactt tggttttgtt tttcataatc ggtttccggc
      781 ggtagaatcg gtttgttttc gagccttaat tttaaccgat tggaggggaa atgtttcccg
      841 ttttccatct ttcaggcgaa agccgccgcc ggatgcttca ggcggcatcg cgttttccct
      901 atgttttcaa agcccgtgcg gaagattcgc acaaagggac tttcggcacg ctcgccgtag
      961 tcggcggatc ggcagggatg agcggcgcgc ccgtattggc ggcatcggcg gcgatgtatc
     1021 tcggctgcgg caaggtgtgg gcgggtttca atcaggaaac gctgccgttt gccgtcattg
     1081 ccggttttcc cgaaattatg ttggacacgg cagacggttt gaccaagcgt caaggcataa
     1141 acgcttggat ggcaggttgc ggcctgggta cggataaagc ggcggtcgga acgcttgccg
     1201 gaattttggc ggaacacacg gacaagcccg tccttttgga tgcggacgcg ctgaacatat
     1261 tatcaaccga tgccgaaacc cgaaatctgg cgcgcgggtg taaaaacctg attttaacgc
     1321 cacaccccgc cgaagccgcg cgcctgcttg gaacgacggt tgcacaggtt caggcggatc
     1381 ggacggcggc agtgaggaag ataagggcaa ttttcggcgc aaccgtggtt ttaaaggggc
     1441 acaaaacatt ggttgcctca cccgatacgg aaatctatgt caacgaaagc ggcaacgcgg
     1501 gattggcaac ggcgggcagt ggcgacgtat tgggcggcat catcggcagt ctgctcgcac
     1561 agggcgtgcc ggtttttgaa gccgcctgcg cgggcgcgtg gctgcacggc gcggcggcgg
     1621 atgtcataaa agaatcggca ggcattgcgg cagggctgtt ggctggcgaa atcgccccgg
     1681 cggcaaggtg gctgcgcaac cggataactg aaagtatgta agaagatata gtggattaac
     1741 aaaaaccagt ac
//
LOCUS       DAWFAN010000222         1734 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWFAN010000222 DAWFAN010000000
VERSION     DAWFAN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.222"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            356..1660
                     /locus_tag="WGO65_001940"
     CDS             356..1660
                     /locus_tag="WGO65_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ0328183.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          357 a          432 c          510 g          435 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cagcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgtgta tttttaagcg ggatgcagat agacgctacc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtgtgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggttat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taaa
//
LOCUS       DAWFAN010000223         1708 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWFAN010000223 DAWFAN010000000
VERSION     DAWFAN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.223"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(37..699)
                     /locus_tag="WGO65_001941"
     CDS             complement(37..699)
                     /locus_tag="WGO65_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0328184.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPPKSMDEIRNQ
                     ASHGAAGLLKLGAGITPEHPDYARWRTEYLDEYDSRYAQDTTLFGGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLAPKLGFIIPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTDSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(712..1539)
                     /locus_tag="WGO65_001942"
     CDS             complement(712..1539)
                     /locus_tag="WGO65_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ0328185.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNAPP
                     LDFPHAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT          334 a          453 c          516 g          405 t
ORIGIN      
        1 atttaatcca ctatatgttt gtggggcgga tatttttcag ggctgtattt tgtccagaca
       61 ttcgagcaga tcgagcggcg tgcggatgtg gaaatccgcc tgccatgagt cggtatcgtc
      121 ttcatctgag atgtagcccc attcggcgag gacggttttc atgccggcgt tgcggcctgc
      181 ttggatgtcg cgttcggcat caccgacgta gagtgtgtgt tgcgggtcgg cgtggatttg
      241 tccgcacgca tacagcatgg gtttgacgct gggcttgggt tcgccgcagg tgtcgccgct
      301 gacgacgacg gcgggtggga tgatgaagcc gagtttgggg gcgagtttgt cggtgaagcg
      361 cataggtttg ttggtgatga tgccccattt gatgccgcgt ctgtcgagtt cggcgatgag
      421 ttcgtttacg ccgccgaaga gggtggtgtc ttgggcgtag cggctgtcgt attcgtcaag
      481 gtattcggtg cgccatcggg catagtcggg gtgttcgggg gtgatgcctg cgccgagttt
      541 gagcagtcct gccgcgccgt ggctggcttg gttgcggatt tcgtccatgc ttttgggcgg
      601 cagtccgtgg cgggcgagca gggtattgag tgcgccgccg aggtctaggg cggtgtcggc
      661 aagcgtgccg tcgaggtcga agagtacggc gtgtatcatg tgtgttcctt ttcagacggt
      721 atttttgttc tgtttcaaac cctgttcgaa atcttccaac atatccaatt cgaagcggct
      781 gaagcccgct ttttcccgcg cttcgatgtt cacatagccg cggaagataa acatatcgta
      841 acgggcaatc agactgcgga acagggcgac aggttccaaa ccgcgttcgc ggcagaggtg
      901 ttgataccaa tggttgccga tggcgacatg acccacttcg tcgcggtaaa tgatgtccaa
      961 tacgccgcag gtttccgaat cgccgcgctg ctccaccttc gcgcgtatcc ccggcgtaac
     1021 gtccagcccg cgcgcttcca acacgcgcgg taccaacgcc atacgcaaca gcgggtcgta
     1081 ggcggttttg tacgccatat cccacaaatg gttgtgcgcc tcgaaactgc cgtaatcgaa
     1141 gccgaaagcg cgcaatctgt cgcgcatcag gcggaaatgg tacacctctt ccttcgccac
     1201 tttcacccag tcgcggacaa actgaaacgg cagcgtgcgg aaacggtatg ccgcgtccaa
     1261 agccagattg acggcgttga actcgatatg cgcaatcgcg tgcagcatcg ccgcatagcc
     1321 ttctgccgtg ttcattttgc gcggggtcag ttgcgacggc gcgaccaaaa ccggcttgtc
     1381 cggccgtccc gcgtggggga aatccagcgg cggcgcgttt gtttcagccc cgtccatgtt
     1441 ctgaacggcg gcaaacgcct catccgtcag ccgtcctttt tcatctgggt cgcccgaaag
     1501 cagggcgcgt tccagcaaag cataaatatc gggtttcatc tcaagtccgc cgtgttcgga
     1561 aaacaaatat tatagcgttt aaaaaaaaca agatgaggca tataatctcc gcgattcggc
     1621 attccgcgcc caaaccgtca aatatagtgg attaacaaaa accagtacgg cgttgcctcg
     1681 ccttagctca aagagaacga ttctctaa
//
LOCUS       DAWFAN010000224         1652 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWFAN010000224 DAWFAN010000000
VERSION     DAWFAN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.224"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            79..1248
                     /gene="metZ"
                     /locus_tag="WGO65_001943"
     CDS             79..1248
                     /gene="metZ"
                     /locus_tag="WGO65_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ0328186.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1343..1549
                     /locus_tag="WGO65_001944"
     CDS             1343..1549
                     /locus_tag="WGO65_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ0328187.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT          404 a          430 c          435 g          383 t
ORIGIN      
        1 ggtttaaatt taatccacta taaattaaac aacgggcgca cgctccgttg ccgaacggat
       61 aaaaccgagg aatcaccgat gagcaaaaaa ctccatccgc aaacgctcgc catacgcggc
      121 ggcaaagaac aaaccgaata ccgcgaacac aatcaggcat tgttcctgac cagcagcttc
      181 atgtgggaca acgcccaaca cgctgccgat ttgttttcaa aaaaaatcaa aggcttcact
      241 tatacccgta ccgccaaccc gactacagcc gcctttgaaa aacgcatcgc cgctttggaa
      301 ggtgcggaac gcgcggtcgc cacttcgacc ggcatgtccg cgattcaggc ggcgtttttc
      361 acctttttgc aggcgggcga ccatgtgatt tccagccgca gcctgttcgg cacgaccgtc
      421 ggctttatca ataacatcgt taccaaattc ggcatcgaag tgagtcacgt gtcgccgact
      481 gatataaacg aatggaaagc cgccgtcaaa gccaacacca aactgctgtt tttggaaacg
      541 ccgtccaatc ccttgggaga agtggccgac ttggaagcct tggcggaatt ggcgcacggc
      601 atcggcgcgc tcttggtggt ggacaacagc ctgctgtcgc ctgtcggctc tcagcctttg
      661 aaacacggcg cggatatttc cgtttcctcc gcgaccaaag ccatagacgg acacgggcgc
      721 gtgatgggcg gcgtgttggc gggttcggaa gaactgatgg cgcaggtcgc cgtttactgc
      781 aactcttgcg ggctggcaat gtcgccgttt aacgcatggc agttgttgag cggcgtggaa
      841 accctgtctc tgcgtatgga aaaacagttc gacaacgcct tgaaaatcgc gcaatggctg
      901 caagcgcagc cgcaggttca agccgtgtat tacaccggat tacccgacca tcctcaggcg
      961 gagcttatcc gcaaacagca aaacggcggc ggcatcgtca tcggctttga agtcgccgac
     1021 caagcagccg cgtggaaagt cgtggacggc gtcgaacttt tctcccgtac cgccaacctc
     1081 ggcgacgtgc gctcgaccat tacccatccg tggacaacca cgcacggcag gatgcagccc
     1141 gaagaaaaac tcgccgccaa catccgcccc ggattggtgc gcctgtcggt cggtttggaa
     1201 tatgttggcg atttgattga cgatttgaaa caggcactcg cgcgctgatt gcgtgttcag
     1261 acggcatcgg cggaaaaaca ccgcaaatgc cgtctgaaac ccttatctta tatgacatac
     1321 acgtcaacaa taggaaaaaa atatggatga agcggttttt gccgattggg cgttgaaaat
     1381 ctgcctgacc ggcttgatta tttttttggg ttttatcgtt tggaatttgg gcaaggagtc
     1441 taaagcgggc aaattcggca tcgccatttt gtttttggtt ttgggcttgg gcgtgtttgg
     1501 ttttgtgttc aaagagttat tgattaagtt tttggtattg ccgaaatagg tttgcggtct
     1561 gtcaaaatgc cgtctgaaat cataaaaagt aatctgcacc ctaaaagttt gatttaaccg
     1621 ccaactgatt aaggtgcgga tttttatgac aa
//
LOCUS       DAWFAN010000225         1649 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWFAN010000225 DAWFAN010000000
VERSION     DAWFAN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.225"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            295..1455
                     /locus_tag="WGO65_001945"
     CDS             295..1455
                     /locus_tag="WGO65_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0328188.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     QNIQQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGATRW
                     GNRESFVGLAGEFGTLRVGRVANQFDDASKAIDPWDSNNVVASQLGIFKRHDDMPVSV
                     RYDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQQTPQIQPTLVPAVVGKPGSDVYYAG
                     LNYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTGGYE
                     EGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKG
                     ENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          408 a          392 c          424 g          425 t
ORIGIN      
        1 gtgccgtgtg tgttttttat ggcgttttaa aaagccggga ctgcatccgg gcagcagcgc
       61 atcggcccgc acgaggtctg cgcttgaatt gtgttgtaga aacacaacgt tttttgaaaa
      121 ataagctatt gttttatatc aaaatataat catttttaaa ataaaggttg cggcatttat
      181 cagatatttg ttctgaaaaa tggttttttg cggggggggg ggtataattg atgacatatc
      241 gggtgtttgc ccgatgtttt taggttttta tcaaatttac aaaaggaagc cgatatgcga
      301 aaaaaactta ccgcgctcgt attgtccgca ctgccgcttg cggccgttgc cgatgttagc
      361 ctgtacggcg aaatcaaagc cggcgtggaa ggcaggaaca tccagctgca gttgaccgaa
      421 ccgctccaaa atattcaaca acctcaggtt actaagcgca aaagccgcat caggacgaaa
      481 atcagcgatt tcggctcgtt tatcggcttt aaggggagcg aggatttggg cgaagggctg
      541 aaggctgttt ggcagcttga gcaagacgta tccgttgccg gcggcggcgc gacccgttgg
      601 ggcaacaggg aatcctttgt cggcttggca ggtgaattcg gcacgctgcg cgtcggccgc
      661 gttgcgaatc agtttgacga tgccagcaaa gccattgatc cttgggacag caataatgtt
      721 gtggcttcgc aattgggtat tttcaaacgc cacgacgata tgccggtttc cgtacgctac
      781 gattcccccg aattttccgg tttcagcggc agcgtccaat tcgttccggc tcaaaacagc
      841 aagtccgcct atacgccggc tcattttgtt cagcagactc ctcaaattca gcctactctc
      901 gttccggctg ttgtcggcaa gccggggtcg gatgtgtatt atgccggtct gaattacaaa
      961 aatggcggtt ttgccgggaa ctatgccttt aaatacgcga aacacgccaa tgtgggccgt
     1021 gatgcttttg agttgttctt gctcggcagc gggagtgatg aagccaaagg taccgatccc
     1081 ttgaaaaacc atcaggtaca ccgcctgacg ggcggctatg aggaaggcgg cttgaatctc
     1141 gccttggcgg ctcagttgga tttgtctgaa aatgccgaca aaaccaaaaa cagtacgacc
     1201 gaaattgccg cgactgcttc ctaccgcttc ggtaatgcag ttccacgcat cagctatgcc
     1261 catggtttcg actttatcga acgcggtaaa aaaggcgaaa ataccagcta cgatcaaatc
     1321 atcgccggcg ttgattatga tttttccaaa cgcacttccg ccatcgtgtc tggcgcttgg
     1381 ctgaaacgca ataccggcat cggcaactac actcaaatta atgccgcctc cgtcggtttg
     1441 cgccacaaat tctaaatatc ggggcggtga agcggatagc tttgtttttg acggctcgcc
     1501 ttcattcttt gattgcaatc tgactgccaa tctgcttcag ccccaaacaa aaatccggat
     1561 acggaagaaa aacggcaata aagacagcaa ataccgtctg aaagattttc agacggtatt
     1621 tcgcattttc ggcttggttt gcacatata
//
LOCUS       DAWFAN010000226         1591 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWFAN010000226 DAWFAN010000000
VERSION     DAWFAN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.226"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            59..1432
                     /locus_tag="WGO65_001946"
     CDS             59..1432
                     /locus_tag="WGO65_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0328189.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          414 a          522 c          384 g          271 t
ORIGIN      
        1 cccatcctat cggtttcccg cttcagacgg cataaggtct gaaagaaaga ctacaattat
       61 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      121 aggttacgaa aacccgacgc ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      181 tgatttacta gccgccgcgc aaaccggcac gggcaaaacc gccgccttta tgctgccctc
      241 gctcgaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      301 tatgctcgtg ttgaccccca cgcgcgaact cgccgaccaa atcgaccaaa acgtgcagtc
      361 ctacatcaaa aacctgccgc tgcgccacac cgtcttgttc ggcggtatga atatggacaa
      421 acagaccgcc gatttgcgtg ccggctgcga aatcgtcgtc gccacagtcg gacggctgct
      481 cgaccacgtc aaacaaaaaa acatcagttt gaacaaagta gaaattgtcg ttttggatga
      541 agccgaccgt atgctggata tgggttttat cgacgacatc cgcaaaatca tgcagatgct
      601 gccccgccaa cgccaaaccc tgctcttttc cgccaccttc tccgccccga tacgcaaact
      661 ggcgcaagac ttcatgaacg cgcccgaaac cgtcgaagtc gccgcgcaaa acaccaccaa
      721 cgccaacgtc gaacaacaca tcatcgccgt cgataccttc caaaagcgca acctgctcga
      781 acggctgatt gtcgatctgc atatgaacca ggtcatcgtg ttctgcaaaa ccaaacaaag
      841 cgtcgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      901 cgaccgttcc caacaaagcc ggctcgaaac actcaacgcc ttcaaagacg gcagcctgcg
      961 cgtcctcgtc gccaccgaca tcgccgcgcg cgggctggac attgccgaac tgcccttcgt
     1021 catcaattac gaaatgcccg cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1081 gcgcgcgggc gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1141 tgaatccatt aaagagctga ccggcaacaa gctgctcatt gagcgcatcg agggcttcga
     1201 gccgcaatgg tgggaacagg gcggcgcaaa accggaaaaa cccgaaatgc gcgaaccgag
     1261 acaacgcaac cgctacgaat ccgccaaagc gcaacgcgaa aaaaacaccc ggccggaaac
     1321 cacggcaaac gatgcgggcg cggcttgcgg aaaaattgcc ggacgcagcc gccgaagccg
     1381 ccgggaacac cggacctgcg ccctgctcca accgcgttac ggcgtaaaat agccctgaaa
     1441 atcaaatgcc gtctgaacat ttccgtttca gacggcattt ttcaaaccgg actgacgcat
     1501 tgggcgcaac cgcccgcacc ggataaattt ctgccgcaaa cagtttcaga cggcatttgc
     1561 tgcctgtaca atatagtgga ttaacaaaaa t
//
LOCUS       DAWFAN010000227         1542 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWFAN010000227 DAWFAN010000000
VERSION     DAWFAN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.227"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            96..404
                     /locus_tag="WGO65_001947"
     CDS             96..404
                     /locus_tag="WGO65_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328190.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFTF
                     DVVQAQALAQAIMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            486..1517
                     /gene="ruvB"
                     /locus_tag="WGO65_001948"
     CDS             486..1517
                     /gene="ruvB"
                     /locus_tag="WGO65_001948"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ0328191.1"
                     /translation="MLQTDNLTAAQPQRIITAQSISSQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAILHKFSGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
BASE COUNT          385 a          518 c          354 g          285 t
ORIGIN      
        1 agttttgagg aggataccca atccaagctt tgcaacagcc gccgccatat ccgctataat
       61 tcacgcttca gccatcctgc ccccgacata aaatcatgac cctgaaaacc gatttattgc
      121 ctaaaatcaa caacgaagat tatcaacgcc tcatcctcaa acacagtgcg gaatttagcg
      181 gtggcgaaat ccgcctgttg aacgaaatcc ttgaaaaatt caccttcgac gtcgttcaag
      241 cgcaggcatt ggcgcaggcg ataatgcagc aagtccgctt cgaccccaac gcctatcaca
      301 tcgacagcga tgacgaagac accacaggca tctgcccaca ctgcatcaat ccgcccatgc
      361 cgcccctgcg cgattacctc gtttggcgcg aaacgcgcgg ataaaacgct tttgaccgtt
      421 atcttttcaa tgccgtctga aacgccgccg accgttcaga cggcataccc gacaaaggga
      481 acactatgct gcaaaccgac aacctaaccg ctgcccagcc ccagcgcatc atcactgccc
      541 aaagcatctc ctcacaagaa gagttgctcg aacgtgccct gcgtcctaaa acgctggacg
      601 actatatcgg gcaagacaaa gccaaagaac agcttgccat tttcatccaa gccgccaaaa
      661 aacgcggcga agcactcgac cacgttttgc tcttcggccc gcccggactg ggcaaaacca
      721 cactggcgca catcatcgcc aaagagctgg gcgtcaacct gcgccaaacc agcggccccg
      781 tcctcgaacg cgccggcgac cttgccgccc ttctgaccaa ccttgatccg cacgatgtat
      841 tgttcatcga cgaaatccac cgcctcagcc ctgttgtcga agaaatcctc tatcccgcgc
      901 tcgaagacta ccggctcgac attatgatag gcgaaggacc cgccgcccgt tccgtcaaaa
      961 tcgacctgcc gcccttcacc ctcgtcggcg cgaccacccg cgccggtatg ctgaccaatc
     1021 cgttgcgcga ccgcttcggc atcgtctccc gccttgagtt ttacgaaaac cgagacctta
     1081 ccaccatcgt cagccgttcg gcacaactgt tgcagctcga tatgtccgaa gaaggcgcgg
     1141 aagaaatcgc caaacgcagc cgcggtacgc cgcgcatcgc caaccgcctg ttgcgacgcg
     1201 tgcgcgattt cgccgacgtg aaaaacaacg gcgtaatcga tgccgccgtc gccgatgccg
     1261 ctttaagcat gctggatgtg gacgcgcaag gtttggatgt tatggacaga aaattcctcg
     1321 aagccatcct gcataaattc agcggcggcc ccgtcggttt ggacaacgtt gccgccgcca
     1381 tcggcgaatc tacagacacc atcgaagacg ttatcgaacc ctatctcatc caacaaggct
     1441 tcctgcaacg caccccgcgc ggcaggatgg cgaccgaacg cgcctacctg catttcgggc
     1501 tgcccgtcga aaaataacgc aatgccgtct gaaacagagc ta
//
LOCUS       DAWFAN010000228         1512 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWFAN010000228 DAWFAN010000000
VERSION     DAWFAN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.228"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            199..1413
                     /locus_tag="WGO65_001949"
     CDS             199..1413
                     /locus_tag="WGO65_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0328192.1"
                     /translation="MAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINLVPFILTAPTP
                     YQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHLSYYDRGRMAN
                     IFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHLNEPKSQKILF
                     IVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEMRELCAYGGLR
                     GFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAGFQEIKTAENL
                     IGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESDIFNHRLKCTE
                     YGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVGNLNETFRYLK
                     QGHVAWLHFKIK"
BASE COUNT          322 a          480 c          365 g          345 t
ORIGIN      
        1 attcacgccc tgctctccaa acaatggacg ctgccgccat tcctgccgaa acggctgctg
       61 ctgtccctgc tgatactgct ggcccccaat gcgatgtttt gggttttggc actgctgacc
      121 gccaccgccc gcccgattgt caatttggac taccttcccg ccgcgctgct gatcgccctg
      181 ccttggcgtt tcgtcaaaat tgccggcgta ttggcgtttt ggctggcggt tttgtttgac
      241 gggctgatga tggtgatcca gcttttccct tttatggacc tcatcggcgc catcaacctc
      301 gtccccttca tcctgaccgc ccccacccct tatcagataa tgaccgggct gttgctgctg
      361 tatatgctgg cgatgccgtt tgtgttgcag aaagccgccg ccaaaaccga cttccggcac
      421 attgccgtct gcgccgccgt tgtggcggca gccggctatt tcaccggcca tttgagttac
      481 tacgaccggg ggcggatggc caatatcttc ggcgcaaaca acttctatta cgccaaaagt
      541 caggcgatgc tctacaccgt tagccagaat gccgacttta ttaccgccgg cctggtcgat
      601 cccgtcttcc tccccttggg caatcagcag cgtgccgcca cgcatctgaa cgagccgaaa
      661 tctcaaaaaa tcctctttat cgtcgccgaa tcttgggggc tgccggccaa tcccgaactt
      721 caaaacgcca cttttgccaa actgctggcg caaaaagacc gtttttcggt ttgggaaagc
      781 ggcagttttc cctttatcgg cgcgacggtc gaaggcgaaa tgcgcgaact gtgtgcctac
      841 ggcggtttgc gcgggtttgc actgcgccgc gcgcccgacg aaaaatttgc ccgctgcctc
      901 cccaaccgtt tgaaacaaga aggttacgcc acctttgcga tgcacggcgc gggcagttcg
      961 ctttacgacc gcttcagctg gtatccgagg gcgggctttc aagaaatcaa aaccgccgaa
     1021 aacctgatcg gtaaaaaaac ctgtgccatt ttcggcggcg tgtgcgacag cgagctgttc
     1081 ggcgaagtgt cggcattttt caaaaaacac gacaagggac tgttttactg gatgacgctg
     1141 accagccacg ccgactatcc cgaatccgac attttcaacc acaggctcaa atgcaccgaa
     1201 tacggcctgc ccgccgaaac cgacctctgc cgcaatttca gcctgcacac ccaattcttc
     1261 gaccaactgg cggatttaat ccaacgcccc gaaatgaaag gcgtggaagt catcatcgtc
     1321 ggcgaccatc cgccgcccgt cggtaacctc aatgaaacct tccgctacct caaacagggg
     1381 cacgtcgcct ggctgcactt caaaatcaaa taacaacaat gccgtctgaa cgcaccaaca
     1441 gccttcagac ggcattttgc agacagaccg acccttcaag cccacttttt tcatcatctc
     1501 cgataaattg ct
//
LOCUS       DAWFAN010000229         1490 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWFAN010000229 DAWFAN010000000
VERSION     DAWFAN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.229"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(128..1120)
                     /gene="porB"
                     /locus_tag="WGO65_001950"
     CDS             complement(128..1120)
                     /gene="porB"
                     /locus_tag="WGO65_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ0328193.1"
                     /translation="MKKSLIALTLAALPIAAMADVTLYGTIKTGVETSRSVYYEDGKA
                     AEVTTGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHEQIDNVKIEKYQIHRLVSG
                     YDNDALHASVAVQQQDAKLVEDNYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFD
                     DADRYNTYDQVVVGAEYDFSKRTSALVSAGWLQEGEGESKFVSTAGGVGLRHKF"
BASE COUNT          374 a          336 c          380 g          400 t
ORIGIN      
        1 agccaaacag aaaaaagcca aacagaaaac agccaaacag aaaacagcca aacagaaaac
       61 aggaaaaagc caaacagaaa aaagcctgtc tggcgacagg ctttttgttg ataccaatct
      121 ttgcagatta gaatttgtgg cgcagaccga caccgccggc agtcgatacg aatttgcttt
      181 cgccttcgcc ttcttgcaac caaccggcag aaaccaaggc agaagtgcgt ttggagaagt
      241 cgtattccgc accgacaacc acttggtcgt aagtattgta gcggtctgca tcatcaaacg
      301 agcctttgaa gccgtgggcg taagaaacgc ggggcgttac gttgccgaag cggtatgcca
      361 aggtagcggc aacttcggtt tgagagttgt gcgaataatt gtcttcaacc aatttggcat
      421 cttgttgttg tacggctacg gaagcgtgca gggcatcatt gtcgtaaccg ctgaccaaac
      481 ggtgaatctg gtatttctca atcttcacgt tatctatctg ctcatgtctt ttataggcac
      541 cgccatattg cacgaagaag ccgccgtttt tgtagttgaa gccggcgtgg taagattcgc
      601 tgttatgtct gcctgcattg tcgttaagcg cgtattgtac gctgccgctg aggccggcaa
      661 attcgggaga atcgtagcgt acggaaatga ggcgtgcctc gggttcggca attttgttta
      721 cacccaaata gtcgcttttg ctatcccaag gattgatgtc gccggtatct ttcaggacgc
      781 tgttcagacg gccgacgcgc aatttaccga agccgccttt caaaccgatg aaggattggc
      841 ggttgcccca accggagtca gtaccggcga tagatgcctt ttgctcaacc tgccaaatgg
      901 ctttcagacc gttaccgagg tcttcttggc ctttgaagcc gattttcgaa cccaaatcaa
      961 cgatgccggt accggttgta acttcagcag ctttgccgtc ttcgtaatat acagagcggg
     1021 aagtttctac gccggttttg atggtgccgt acagggtaac gtcagccatt gctgcaatag
     1081 gaagggctgc caaagtcagg gcaatcaggg attttttcat tgctgtattc cttttttggt
     1141 taagaaattt aagcaggccg ggctttccaa gccgcttagc tttgcattta ccgccgacgt
     1201 tttcggcgat tccatgtctg aaactataga tgttcctgtc tgaaaaaaca aacttctgcc
     1261 tgtttttgtg tggaattcga tgtgtatttt gaagggcgga tagagatatt gtgggtattt
     1321 tttgttttat aacatatggt tatttaaatt ttttaagatt tgcattttta caacacttac
     1381 tcgggagggt attggagggc attgcaaacc gagggttata aagacggcaa aaaacgcgca
     1441 cgtggtttct ttcggaacgg gcggatgcaa aacccgcccc tgcggcagga
//
LOCUS       DAWFAN010000230         1455 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWFAN010000230 DAWFAN010000000
VERSION     DAWFAN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.230"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(127..1374)
                     /locus_tag="WGO65_001951"
     CDS             complement(127..1374)
                     /locus_tag="WGO65_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0328194.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPIIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKAAAVK
                     SRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          327 a          388 c          417 g          323 t
ORIGIN      
        1 aaacacagtc aaacaaattt atatgcccca tcccttccga ataatttgaa aacacagccg
       61 ccaaaaacaa aaatgccgtc tgaaaacctt tcagacggca tttccaactt gatttcaggc
      121 agaaagtcag aacgcgatat agctgttcgg attaaccggt ttgccgtttt gccgcacctc
      181 gaaatgaagc tgcgttctag aagcgtcggt attgcccatc aaagcgacct gctgcccgcg
      241 tttgacctgc tggccttcgc cgaccagcaa tttttggttg tgcccgtatg cggtcaggaa
      301 ggaagaatta tgctggatga tgaccaaatt gccgtatccc ctcaaaccgg aacctgcata
      361 aaccactttg ccgtcagccg ccgccaaaac gggctgtccc gcatttcctg caatatcgac
      421 acccttattg ttgccgccga aatcggcaac cactttacct tgcgtcggac gctgccaaac
      481 aatgccgccg accgaacgcg tgccggaagg cgaagcggca ggagattgcg gggcgggcgc
      541 gggaaccgct ttattttccg cagcgggcgc ggcaggttgc ggcgcggact gcacaggcgg
      601 ttgcgcggcg ggtttcacag gggtttgcac ggcagccggt acggcgggcc tgctttctac
      661 ggctgaggtt ttcggtgcgg catatcctgc cggtttgact ttaacaatct gaccgatgct
      721 caacatattg tcggtcatgc cgttccacgc acggaaatcg tcttgagaga tatggtagcg
      781 tttggaaatg ttgtacaccg tgtcgccgcg cacaatagta tgcgtcgccg cgttgatgtc
      841 gacgggtgcg gactgtacgg gcggttgcgc ggcagccggt acggcgggcc tgctttttac
      901 ggctgcggct ttcggtgcgg catatcctgc cggtttgact ttaacaatct gaccgatgct
      961 caacgtattg tcggtcatgc cgttccacgc acggaaatcg tcttgagaga tatggtagcg
     1021 tttggaaatg ttgtacaccg tgtcgccgcg cacaatagta tgcgtcgccg cgttgatgtc
     1081 gacgggtgcg taagaaggaa cgtatgtacc cgaaacggca ggtgcagacg gcggaacata
     1141 agcaggaggc gtataaaccg gcgcgctttg caccggcggc acataaggcg catcgccggc
     1201 aggagccggg ctgtacggcg ttgctccata ggggttgttg taaactgccg aagacggcgc
     1261 gtcctgcata cctgaattgc ctgcaatgat gggggcaggc tgctgggtag cacaaccgcc
     1321 caacagtgcg gcaaaggagg cgcaagccgc caaaagtgtc gtttgtttca acataagata
     1381 accttcatgt tccgatatat agcctgaatg cggtatatca taataaaaat gcgcgttctt
     1441 ctcaagcgca aagcc
//
LOCUS       DAWFAN010000231         1445 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWFAN010000231 DAWFAN010000000
VERSION     DAWFAN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.231"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            27..1364
                     /locus_tag="WGO65_001952"
     CDS             27..1364
                     /locus_tag="WGO65_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0328195.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGQNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGIWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
BASE COUNT          259 a          347 c          391 g          448 t
ORIGIN      
        1 tatcttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtgct tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
      121 atacggcagg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
      181 tggtcgccct gcccgtccag cttgccgagt tttatatcgg gcgcacaggc ggtcaaaacg
      241 ccgtcgattc cttcagggct ctgcgtccga gtacgcaatg gctttgggtc gggcgcatgg
      301 gcgtttccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattaa
      361 attatgtcgt ccacagtttc acgggggcgg ttcataccgg cgcggacttt gaagccttgt
      421 tcggcgcgac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtctg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggc caggcgtttt ttgccttgag catcggcgtt tccgctatga
      721 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg ccggcagtgt
      901 ttatgaagat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcgttt tcgatgttgg aaacggtcat tgcctcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa cacacttggc ttatcggcac ggctattttc attgtcggca
     1081 tcccgtccgc gctgtctttc ggcatctggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactatgtt atttccgccg tcattatgcc tgtcggcgcg ttgggcgttg
     1201 ccgtatttac cgcctggatt caggacaagc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac cttgcgctac ctcgccccga
     1321 ttgccattat tattgttttc gtcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 tcaaa
//
LOCUS       DAWFAN010000232         1416 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWFAN010000232 DAWFAN010000000
VERSION     DAWFAN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.232"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            188..1372
                     /gene="ubiM"
                     /locus_tag="WGO65_001953"
     CDS             188..1372
                     /gene="ubiM"
                     /locus_tag="WGO65_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ0328196.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT          379 a          409 c          308 g          320 t
ORIGIN      
        1 ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca tacaccctcc
       61 cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac agatagttca
      121 aacggcattc aaacccttac aaataccgtc tgaaatcatc ggcatccgtc tatgcaaagg
      181 atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggcccgg cgggtttaag
      241 ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgattgaac gcagcccact
      301 gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc atttttcccg
      361 cgaaatcatg cagcgtttgg gtatgtggga caaaattccc gaaaacgaaa tctacccttt
      421 gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc cccaaccgac
      481 cgaggcgcgc ggcgaacctg ccgactgttt gggctatctt atttccaacc acaatatccg
      541 acgcgctgcc tacgaagtcg tatcccaact cgacaacatt accattctga ccgatacggt
      601 tgtcaaagaa gtcaaaacat ctgacaacga agcacaagtc attttggaaa acggcaaaat
      661 cctgactgca cgcctgcttt tggcggcaga cagccgcttc tcgcaaaccc gtcgacaact
      721 cggtatctcc tccgatatgc acgattacag ccgaaccatg ttcgtctgcc gtatgaaaca
      781 taccctttcc aaccaacata ccgcttacga atgcttccat tacggacgca ccatcgcact
      841 gctgccgctg gaagagcacc tgaccaatac cgtcattacc gtcgataccg ataaaatcaa
      901 cagcgttcaa aacctttcgc ccgaagaatt ggcggcaagc gtaaaagaac agctcaaggg
      961 caggcttggc gacatggaac tggtcagcag tatccaccat tacccccttg tcggtatgat
     1021 tgccaaacgt ttctacggta aacgcagcgc gctgatcggc gatgcggctg tcggtatgca
     1081 ccccgttacc gcacacggtt tcaacttggg tctgtccagc gcagatattt tggcaaaact
     1141 gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt tggaaaaata
     1201 cagcagcaaa catatgctgc atgcccagcc gctttatcac ggaaccaata tgatgctgaa
     1261 actgtttacc aatgagaccg cgccggcaaa actgctgcgc ggtttggtat tgcgtgccgg
     1321 caacaacttc ccgccgctga aaaaactgat taccaaacaa ctgaccggtt gaaaactgcc
     1381 gaaatgccgt ctgaaacgtt tcagacggca tttttg
//
LOCUS       DAWFAN010000233         1332 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWFAN010000233 DAWFAN010000000
VERSION     DAWFAN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.233"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..138)
                     /locus_tag="WGO65_001954"
     CDS             complement(<1..138)
                     /locus_tag="WGO65_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0328197.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEHYLNRQRTR
                     YL"
     gene            423..1094
                     /locus_tag="WGO65_001955"
     CDS             423..1094
                     /locus_tag="WGO65_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0328198.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDDSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
     gene            1156..>1332
                     /locus_tag="WGO65_001956"
     CDS             1156..>1332
                     /locus_tag="WGO65_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0328199.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLS"
BASE COUNT          367 a          308 c          306 g          351 t
ORIGIN      
        1 gaggtaacgg gttctttgac ggttcaggta gtgttcgatc ggctgccaat caattacctg
       61 atccaacttc aatagtggga agcggtcgat gtgtttggca atcatggctt gtgcggtttg
      121 ttgaaagaag gtgctcatga gaaatcccct aaatgtcttg gtgggaattt aggggatttt
      181 ggggaatttt gcaaaggtct caaacggatg aaatcaaccg atattcaaac acagtcattt
      241 ttagcgcatt ttcagcgtat cgttaatgcg gaaaatttcg tgaacaggtt ttttgcacag
      301 gtctccgcct cgtttcgcgg gatgggaaac cgtattagaa aacggacgca cgcaacataa
      361 accccgaaag tgatgataag atgatgattt aacgtactgc tttaattatt taaggaatta
      421 tcgtgtttcc cgacaaatac aagttgagtt tagaagaaaa tatttttctg gcaaagaaag
      481 tattggttgc ccaaattcac aacctcagcc gttttgagaa ttgtcagacg accttgttgc
      541 agaccgaaca aattatccat ggcaaaaatg tagcctccgc gtcactggaa gacatccaaa
      601 ccatcttgaa cctgaaacgt gcctatcaat atgtgatttc gcatatttca aacggcgaac
      661 cggtcgatat ttcactcctt aaaaaaatca acaacattgt tgccaaggac gattctttgg
      721 cacccggtga tttccgtacc ggttcggtcg gcgtaacgct attggacgat tcccgtcatg
      781 ccccaaatcc ggtgaaggaa attgaagtgg ctcgcgtgtt acaaaatatc ggactgcaaa
      841 gcggttcgac gacggaggca gccgtccgtt ttatgcttta ttgtatgcgg cagcaggttt
      901 tttgggacgg caacaaacga acggcaacct tatttgccaa cggtctgatg atggcggggg
      961 gctgcggcat cttggaaatc tccgaaatgc agatgccgca attcaatgaa aaactgtccg
     1021 cattctatcg ctccggcgac gataccgata tttccaagtt tgtgtatcaa aattgtatat
     1081 cgggcataga ctgagacctt tgcaaaattc cccaaaatcc cctaaattcc caccaagaca
     1141 tttaggggat ttctcatgag caccttcttc cggcaaaccg cccaagccat gattgccaaa
     1201 cacatcgacc gcttcccact attgaagttg gatcaggtaa ttgattggca gccgatcgag
     1261 cagtacctga accgtcaaaa aaaccgttac ctccgagacc accgcggccg tcctgcctat
     1321 cccctgctgt cc
//
LOCUS       DAWFAN010000234         1307 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWFAN010000234 DAWFAN010000000
VERSION     DAWFAN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.234"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(99..1022)
                     /locus_tag="WGO65_001957"
     CDS             complement(99..1022)
                     /locus_tag="WGO65_001957"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ0328200.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          350 a          311 c          330 g          316 t
ORIGIN      
        1 gggaatgacg aattttaggt ttctgttttg gttttttgtc cttgtaggaa tgatgaaaat
       61 ttaagtttta ggaatttacc ggaaaaaaat agaaagcgtt atccacaagt tctgatgttc
      121 agctcgtgaa atgcgtcggg caaatcatcg ctgtcggcaa attccacccg gtcgtaagcc
      181 gtttcgtctg ccaaaaccgc gcgcaagagt gcgttgttga tggcgtgtcc cgatttgtag
      241 ccttcaaatg cgccgacaat cggatgtccg acgatataca aatcaccgat ggcatcaagg
      301 attttgtggc gcacaaactc atcgggatag cgcaagcctt caggattcag gacatccgtg
      361 tcgtcaatca cgatggcgtt gttcaaattg ccgcccaaac ccagattgtg ggcgcgcatc
      421 atttccactt cgtgcataaa gccgaaagtg cgcgcgcgcg cgatttcgtc gatgtaggat
      481 ttgccggcga aatcgatttc aaaagtgggc gagctgcggt tgaaaaccgg atggtcgaat
      541 tcgatggtca gcgttacctt aaagccgtca tacggcgtaa agcgcaccca tttgcccgct
      601 tctttgattt cgacaggctt gaggattttc aaaaaacgct tttgcgcctt ttgatcgacc
      661 acgcccgcat cttgcaaaag gtaaataaac ggcaggctgg agccgtccat aatcgggatt
      721 tcgggcgcgt tcagctcaat cagcgcgttg tcgataccgt aggcggacag cgcggacata
      781 atgtgttcga tcgtgccgac gcgcacgcct ttgtcggtaa cgatggtgga ggaaaggcgg
      841 gtatcgttga tcaaataagg ggtcagtttg atttgttcgc ccatctcgcc gtccaaatcg
      901 gtacggcgga aggaaatccc gctgttttca ggcgcggggt gcagggtcag tgcgacccgt
      961 tcgccggaat gcagcccgac tccggtaacg ctgatggatt tcgccaaagt tctttgcagc
     1021 ataaaccgct tccttatcaa tggggtaagt tttggaataa tacgataaaa ccggaaaaac
     1081 aggctatgtt tttccatagt atttgccaat gtatccgttt tcaatacgta agccgcataa
     1141 aaatgtatag tggattaaca aaaatcaaga caaggcgacg aacccgcccc tcccgaaaaa
     1201 cgcaaaaaat gccgtccgaa aacctttcgg acggcatttt cgcgtgaacc gtcattccca
     1261 caaggacaaa aaaccaaaac agaaaaccaa aaacagcaac ctaaaat
//
LOCUS       DAWFAN010000235         1296 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWFAN010000235 DAWFAN010000000
VERSION     DAWFAN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.235"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..424)
                     /locus_tag="WGO65_001958"
     CDS             complement(<1..424)
                     /locus_tag="WGO65_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0328201.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRMVINHHLALAADEVFEGSVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLD"
     gene            complement(544..750)
                     /locus_tag="WGO65_001959"
     CDS             complement(544..750)
                     /locus_tag="WGO65_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328202.1"
                     /translation="MFDGAKVVRRVEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
     gene            1040..1183
                     /locus_tag="WGO65_001960"
                     /pseudo
     CDS             1040..1183
                     /locus_tag="WGO65_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(1114..>1296)
                     /locus_tag="WGO65_001961"
     CDS             complement(1114..>1296)
                     /locus_tag="WGO65_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEZ0328203.1"
                     /translation="AKAKAIAEYRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGA
                     PAPKGPAKPALPAAKK"
BASE COUNT          311 a          365 c          263 g          357 t
ORIGIN      
        1 cgtccaactt gtcgcagctg ctcagactat cggtataaac aatactgtcc ggcatgattt
       61 tctttttgat gacagggagt aacgtttcag acttggcatt atccaccaca acggtataga
      121 cccgtccgtt gcgtttcaga atgccgaaga caaccacttt tcctgccgca ccgcgaccac
      181 gtctgccttt acgccgtccg ccgaaatcgc tttcgtccgg ctcgacagag ccctcaaaaa
      241 cctcatcggc agccaaggcc aaatgatggt tgataaccat gcggatttta cggtagaaca
      301 gtgctgccga attgggatgg atacccaaaa tatcggcggc agaacgggcg gtaacttcga
      361 gtacaaaaaa acggagcagt tctttctgta cttttttctt taatttgcag tgcgttatct
      421 tcatatttcg agggtaacat atctgctaat ctagtacagc ccctagttta aagctttgtt
      481 tcttaagtcc gcagagtatg ccatggttag accttcaaag ttgagtattg tactattttg
      541 tttttaatta actataaaac gcttgtgtga ataggaagac tgcagaatat tgctgccttt
      601 tcctgtttgc tttaaataaa cagaaaatgc cgtctgaaac cgattttcag acggcatttg
      661 tcccttaaaa cacgtttttt caagcgcgac cataccaaac acccacaggt tgccttccac
      721 ccttcgtaca acctttgctc catcaaacat ccacttccta aatactgatg tctgcttagg
      781 ttgaatcggc attcgtacga ttacggcaca ccgcctaatc tgtacctgcg tctgttgcgc
      841 ccaataccaa agactacctg aaaatctccc aaacttttca ggtagcctta tcacgttttc
      901 gactgttacc acacgcccaa agaccgcctg aaaccctttt tcagacgttc cgctccgata
      961 ccccccaaca aaacgccgcc cgaacgcatg ttcagacggc attttctttg cgtcgattca
     1021 ttccccgata tagcgcaggt ctttttccag ccatttccct tccaaacaaa aaagcaccga
     1081 cggcggccga tgccccttcc tttacaggtt cccctatttt ttagctgcgg gcagagccgg
     1141 tttggctggg ccttttggtg cgggcgcgcc gacggaagcc tgatccttca gcttcgccag
     1201 caccgcaggg ccgatgccct ttaccttggt caaatcgtct acagacttga acgcaccgtt
     1261 ttgcgcacgg tattccgcaa tggccttcgc cttcgc
//
LOCUS       DAWFAN010000236         1274 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWFAN010000236 DAWFAN010000000
VERSION     DAWFAN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.236"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            327..>1274
                     /locus_tag="WGO65_001962"
     CDS             327..>1274
                     /locus_tag="WGO65_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0328204.1"
                     /translation="MNKTLKRRVFRHTALYAAILMFSHTGGGGAQAQTRQYAIIMNEG
                     NQPEVQWNGSYSIKDKDRKREYTHHNHSRGGSSVSFNNSDELVSQQSGTAVFGTATYL
                     PPYGKVSGFDADGLNKRGNAAGWIRTTRIALAGYSYEGIVCRSGTGCPKLVYKTRFSF
                     DNPDLVKNAGRLDRHTDPSRENSPIYKLKDYPWLGVSFNLGAEGTTKDGKTINKLVSS
                     FDEKNSSNNNLVYTTEGRDISLGDWQREKTAMAYYLNAKLHLLDKKGIKDITNKTVQL
                     GVLRPSIDVRLQRNTGLAGLLNFWASWDIKDNGQIPVKLG"
BASE COUNT          342 a          316 c          348 g          268 t
ORIGIN      
        1 gtcgggcatc tgcagcggtt tgctaaaaaa cgctttaccg tgatcagtgt gcaaagttaa
       61 aatggggagg taagcttttc aatcagcaat ccggcgggcg cgggatcggg cggtttgccg
      121 aatcccgccg tttgcggcgc gcctgccgcc gacggtatcc cgcgaagcaa gatttaaggg
      181 ataaaatatg ttccaacacg cagggcggca cataaggcgc cgccctgatt cggaaggggc
      241 tgcatccctc ccggacaaag cctgatcctg ccgccccgaa ggacggatgc ccgaagggcg
      301 gggggtttga ccgaaaagga aatacgatga ataaaacttt aaaaaggcgg gttttccgcc
      361 ataccgcgct ttatgccgcc atcttgatgt tttcccatac cggcgggggg ggggcgcagg
      421 cgcaaacccg tcaatacgct attatcatga acgagggaaa ccagcccgag gtacagtgga
      481 atgggtcata ttcaataaag gacaaagaca ggaagcgcga atatactcat cataatcact
      541 cacgaggagg aagctctgtc tcattcaaca atagcgatga gcttgtttct caacaaagcg
      601 gtactgccgt ttttggcaca gccacctacc tgccgcccta cggcaaggtt tccggttttg
      661 atgccgacgg gctgaacaag cgcggcaatg ccgccggttg gattcgtacc acccgcatcg
      721 cgctggcagg ctacagctac gaaggtatcg tatgcagaag cggcacaggc tgtcccaaac
      781 ttgtctataa aacccgattt tccttcgata atcccgactt ggtaaaaaat gcgggcaggc
      841 tggataggca cacagaccca agccgcgaaa attcgcccat ttacaaattg aaggattatc
      901 catggttggg cgtatctttc aatttgggtg ccgagggtac caccaaagat ggtaagacaa
      961 tcaacaaatt ggtatcttct tttgatgaaa agaatagtag taataacaac ctcgtctata
     1021 ccacggaagg ccgcgatatt tccttgggcg actggcagcg cgaaaagacc gccatggcct
     1081 attatctgaa cgccaaactg cacctgctgg acaaaaaagg gattaaagat atcaccaaca
     1141 aaacagtgca gttgggtgtc ttgaggccga gcatcgatgt gcggctgcaa agaaatacgg
     1201 ggcttgccgg cttgctaaat ttttgggcta gttgggacat taaagataac gggcagattc
     1261 cggtcaagct cggc
//
LOCUS       DAWFAN010000237         1266 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWFAN010000237 DAWFAN010000000
VERSION     DAWFAN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.237"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            41..373
                     /locus_tag="WGO65_001963"
     CDS             41..373
                     /locus_tag="WGO65_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ0328205.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            705..1214
                     /locus_tag="WGO65_001964"
     CDS             705..1214
                     /locus_tag="WGO65_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ0328206.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          337 a          240 c          304 g          385 t
ORIGIN      
        1 gccttgtttt gacggaaaac catcatataa aggaacactt atgcagattt tatcttttca
       61 gccggacatt gcggaacgta tgctggaagg tacggaaggc gagtcggtca acgaaaacgc
      121 acaattcgtc cgtacggaca acggctattg gattgcgtgg catgaaggcg tagcggcact
      181 gcttgcgccc gatacgccgc cgggcattcc ctgtttttgg gtggaaggcg cggaaagcct
      241 tgaagagttg tgcgtcatgg tggaacgcgg cgagtttgac gaagtggaag agtttgacgg
      301 cgatgacgac gaatggctcg aaacggcaca gggttgcggg caccacggcg acgcttgcgc
      361 ctgcggacat taaaggcatt gcaggcttgc cgcaaggggg gcaaggcttt gccgtttttt
      421 aaataatcgg tggattgcat ctgtttgcaa tgggctgaac gcttcccttt gtattttatt
      481 gaataaaaaa acttatcttg gtattataat taaggcagca tcaattattt tgggatggca
      541 ataaacgcaa agcattgatt tgcgccgatt gcagacttat tatagcaggt tgcggcgcgg
      601 acttaatggg aattgtttat agagcatgat ggtctgaaac atcattaatt aaaaatacca
      661 ttaatccgat ttatatttat ttcaatttca atggaaaaac atcaatgaca atgattttaa
      721 gcattttaag cctgtttttt atcatcagac tgttattttt agccgtctct attaaacatg
      781 aaaaagcctt gattgccaaa ggggcgaaac aatacggaaa aaccaattcc acgctgcttg
      841 cggcagttca tacgctttat tatttggcgt gttttgtttg ggtatggctt tctgacactg
      901 cttttaatgg catatccttg attggtacgc tgacggtgat ggcttcgttt gtgatattgt
      961 cattgattat taagcagttg ggggagattt ggacggttaa aatctatatt ttaccaaatc
     1021 atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca ccccaattat tttttaaaca
     1081 tcatacccga actgattggc atcgccttat tatgtcaagc gtggtatgtt ttattgattg
     1141 gtctgcccat ttatttgctg gtcttattta agcgtatccg acaagaagaa caggcgatgg
     1201 caacactttt ttaacccgtt tcatcaatta tagcggatta acaaaaacca gtacggcgtt
     1261 gcctcg
//
LOCUS       DAWFAN010000238         1253 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWFAN010000238 DAWFAN010000000
VERSION     DAWFAN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.238"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            236..1189
                     /locus_tag="WGO65_001965"
     CDS             236..1189
                     /locus_tag="WGO65_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0328207.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          200 a          271 c          432 g          350 t
ORIGIN      
        1 aaaccggatg attcgggcta aataattcct gattctttgg atatagccaa cggtatgcct
       61 gccgatgccg tcagaaaggg cttgatgggc gtttgcggtt cgggttaata tttgtgctgt
      121 atctgttaat tccggttagt aaaatggggc ggggacgatg agtggccgtc ggtcggtttt
      181 gcacgggcgg taataatgtc cggcgcaagg tgcgccgttt cgggaggggt gtgtgatggg
      241 ttttctgatt ggtttgagcc gcaacctgac gcggtttacg gctttggtca ttttgcttgc
      301 gtcggcggtt gcgtttatcg gaccggcttc gtttgcgtgg gttaagggcg atgttcaggt
      361 cttggtgctg ggcatcatta tgctgggtat ggggatgact ttgggcaggg aggactaccg
      421 gattttggcg cagcgtcctt tggatgtgtt tatcggtgcg gcggcgcaat ataccgtgat
      481 gccgttgctc gccatcggta ttgccaagct gttcgacctt tcgccggggc tgactttggg
      541 gctggtgttg gtggggacgt gtccgggcgg cgtgtcgtcc aacattatga gtttccttgc
      601 caaaggcgat gtggcgtttt cagtcggtat gacgacggtg tctacggtgc tggctccggc
      661 cgttacgcct ttgtggatga cctgcctggt cggtcagacg gtggatatgg acggttgggg
      721 gatgttccgg tttatgctgc tggtcacgct tttgccggtg gtgctgggtt cggcggcaaa
      781 tatcctgctg cacggaaaac attggtttga aaatgtccgt gccgttatgc cgggcgtggc
      841 ggtattggca tttgcctgca ttgtcggcgg tgtggcttcg gttcacggac accgttttgc
      901 cgaatcggcg ttggtaatgg tgttggcgat tgcggtacac aatatcgccg gctatgtgtc
      961 aggctattac gccgccgcgc tgtttggtat ggatacggca aaaaaacgca ccttggcaat
     1021 cgaagtcggc gtgcagaatg ccggtttggc aacaggattg agcgcgaaat ttttcccagg
     1081 caaccccgaa tcggcggttg cggcggcggt cgcctgcgtg tggcattcgg tttcggggac
     1141 ggtattgggc aacttgttcg ctctgtggga caagcgtcgg gacgcttagt cgggcgtgtc
     1201 cgaaccgtcc cacgatatat agtggattaa ctttaaaccg gtacggcgtt gcc
//
LOCUS       DAWFAN010000239         1223 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWFAN010000239 DAWFAN010000000
VERSION     DAWFAN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.239"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..494
                     /locus_tag="WGO65_001966"
     CDS             <1..494
                     /locus_tag="WGO65_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0328208.1"
                     /translation="KGNNITLFIADDVEIMGLVCSLHSDCSLQIQAKTTMGNGEITIA
                     EKGKISIGKDCMLAHGYEIRNTDMHPIYSLENGERINHGKDVIIGNHVWLGRNVTILK
                     GVCIPNNVVVGSHTVLYKSFKEPNCVIAGSPAKIVKENIVWGRKMYHSTMYDDPTLNE
                     FYK"
     gene            545..1222
                     /locus_tag="WGO65_001967"
     CDS             545..1222
                     /locus_tag="WGO65_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HEZ0328209.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
BASE COUNT          408 a          206 c          256 g          353 t
ORIGIN      
        1 taaaaggaaa taatataacc ttatttatag ctgatgatgt agaaatcatg ggactagtat
       61 gttcccttca ttcggactgc tctttacaaa ttcaagccaa gaccactatg ggcaatggag
      121 aaattaccat tgctgaaaaa ggtaagatta gtattggcaa agattgtatg ctggcacatg
      181 gatatgaaat tagaaatact gatatgcatc caatttattc tttagaaaat ggcgaacgaa
      241 taaaccatgg taaagacgtt attatcggta atcacgtgtg gcttggccgc aatgttacta
      301 tattaaaagg tgtatgtatt ccaaataatg tagttgtagg ttctcatact gtgctatata
      361 aaagttttaa agaacctaat tgtgtaatag caggttcacc tgcaaaaata gtaaaagaaa
      421 atattgtatg gggaagaaaa atgtatcatt caactatgta tgatgaccct actttaaatg
      481 aattttataa ataacttagg ggcttaggaa gcaaaatcat caaaaaacct gataagctcc
      541 taatatgaaa ctaaaaaata agtatcaaaa gttcagcaaa atttcagagc aaaagtttag
      601 agaaataatc cgctgtttcg ctcttgattt gaccgcttcc gatactgcta aaatgaccgg
      661 cattagtgta cgcggtatca atcctatttt cctgaaaatc agacacagga ttgctgctct
      721 gtgcgaacaa agctcaccac tgtccggtgt ggtcgaatta gatgaatctt actttggtgc
      781 gcgacgtatc aggggtaagc gcggtcgcgg agcgtcaggt aaaaccatcg tatttggcat
      841 actgaaacgc aatggcgtgg tctatacgga aatcgttccc gacgcttcga aagcctcact
      901 aatcaaggtc atacgtggtc acatatctgc tgacagtgaa atcaatactg acggctggaa
      961 agcatatgac ggtcttgtcg atatgggcta tgagaagcat taccgtgtcc atcatggttc
     1021 caatgagttt gctcgaggaa agcaacatat caatggtatt gaatcttttt ggagttatac
     1081 taaggggcgt ttggctaaat ttcatggtat ttccaaagag atgttttatc tgcatctcaa
     1141 agaaacggag tttaggttta accaccggca tgaagattta ggtaaaatat taatgaagat
     1201 gcttcgaaat aacccaattt aat
//
LOCUS       DAWFAN010000240         1208 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWFAN010000240 DAWFAN010000000
VERSION     DAWFAN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.240"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>1208
                     /locus_tag="WGO65_001968"
     CDS             <1..>1208
                     /locus_tag="WGO65_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0328210.1"
                     /translation="FAKHGYAALAELDSNGDNIINAADAAFQTLRVWQDLNQDGISQA
                     NELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKMGDLLLAADNLH
                     SRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKEAQLALL
                     DNLIHKWAETDSNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSDKAKA
                     AIDAARDRIAVLDAYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAKNIYQNLLFQTR
                     LQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEG
                     RRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGHNKNVSLY
                     GNDGNDTLIGG"
BASE COUNT          373 a          275 c          272 g          288 t
ORIGIN      
        1 tttgccaaac acggctacgc ggctttggcc gaattggatt caaacggcga caacatcatc
       61 aacgcggcag acgccgcatt ccaaaccctg cgtgtatggc aggatctcaa tcaggacggc
      121 atttcccaag ctaatgaatt gcgtaccctt gaagaattgg gtatccaatc tttggatctc
      181 gcctataaag atgtaaataa aaatctcggt aacggtaaca ctttggctca gcaaggcagc
      241 tataccaaaa cagacggtac aaccgcaaaa atgggggatt tacttttagc agccgacaat
      301 ctgcacagcc gcttcaaaga caaagtggaa ctcactgccg aacaggcaaa agccgccaat
      361 cttgcgggca ttggtcgtct gcgcgatttg cgcgaagctg ccgcattgtc cggcgatttg
      421 gccaatatgc tgaaagctta ttctgccgcc gaaactaaag aagcacagtt ggcattgtta
      481 gataatttga ttcacaaatg ggcggaaacc gattcgaact ggggcaaaaa atcgccaatg
      541 cgactttcaa ccgattggac gcaaacggct aatgaaggta ttgcactgac accatcccaa
      601 gtagcacaac taaaaaagaa cgctttagtt tccctttctg ataaagctaa agcagctatt
      661 gacgccgccc gcgaccgcat tgccgtgctt gatgcctaca cggggcagga ttccagcaca
      721 ctctattaca tgagcgaaga agacgcgctt aatatcgtca aagtaaccaa cgatacatac
      781 gaccatctcg ccaaaaacat ctaccaaaac ctgttattcc aaacccgttt gcagccatat
      841 ttgaatcaaa tcagtttcaa aatggaaaat gatacgttca ctttggattt tagtggtctt
      901 gttcaagcat ttaaccatgt caaagaaact aatccgcaaa aagcttttgt ggatttggcc
      961 gagatgcttg catatggcga acttcgttct tggtatgaag gccgaagact aatggccgat
     1021 tatgtggagg aggcaaaaaa agcaggtaaa tttgaagatt accagaaagt gttgggtcag
     1081 gagaccgttg cattattagc taaaacatcg ggtacgcaag cagatgatat cctgcaaaat
     1141 gtaggctttg gtcataataa aaatgtttct ttatatggta atgacggtaa cgacactcta
     1201 atcggcgg
//
LOCUS       DAWFAN010000241         1167 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWFAN010000241 DAWFAN010000000
VERSION     DAWFAN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.241"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(57..215)
                     /locus_tag="WGO65_001969"
     CDS             complement(57..215)
                     /locus_tag="WGO65_001969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328211.1"
                     /translation="MAENLPFPSFIHLLFLEFAFTGRGGAPRTPGFGKPPDSAPAELL
                     VEKLTSPF"
     gene            301..>1167
                     /locus_tag="WGO65_001970"
     CDS             301..>1167
                     /locus_tag="WGO65_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilC"
                     /protein_id="HEZ0328212.1"
                     /translation="MNGQKLPEVKWGQSYNSLPQKSNPRQVNFTTSFGGYKKNTTFSF
                     NNTDEVVAQKNGVTVFGAATYLPPYGKVSGFDEKRLKERGDVLGWIGTTRPGLVGYSY
                     EGNTCSSGDCPDVSYKTQFTFGNQGLKSKVNGKLDIYEDKSRDNSPIYKLPDQPWLGV
                     SFNLSSESVVESKKLKKVESSFSEDVTQSNGAQSLYKYKNLVYTTGDVRNKGNQVHQD
                     KHHAVAFYLNAKLHLLDKKNIQNIAQGITVNFGTLKTRIEPTDAWKNKRHLTHNVGNW
                     EFKDTGSVSVKLK"
BASE COUNT          347 a          268 c          292 g          260 t
ORIGIN      
        1 gttgggcatc tgcagcggtt tgctaaaaaa cgctttaccg tgatcagtgt gcaaagttaa
       61 aatggggaag taagcttttc aaccagcaat tcggcgggcg cggaatcggg cggtttaccg
      121 aaccccggcg ttcgcggcgc gccgccccgt cccgtgaagg caaactcaag gaataaaaga
      181 tgaataaaac ttggaaacgg caggttttcc gccataccgc gctttatacc gccatattga
      241 tgttttccca taccggcggg ggggggggcg caagcgcaaa cgtatccata cgctcttgtg
      301 atgaacggac aaaagctgcc cgaggtaaag tgggggcaat catataattc attgccgcaa
      361 aaaagcaacc cccgccaagt taactttacg acatcttttg gtggatacaa aaaaaacact
      421 actttctcat tcaataatac cgatgaagtt gttgctcaaa aaaacggtgt taccgttttc
      481 ggcgcggcga cctacctgcc gccctacggc aaggtttccg gttttgatga aaagaggctg
      541 aaagagcgcg gcgatgtcct tggttggatt ggtacgaccc gcccggggtt ggtaggctac
      601 agctacgaag gtaacacatg ctccagcgga gactgtcctg atgttagcta taaaacccaa
      661 tttaccttcg gtaatcaagg gttgaaaagc aaggtaaacg gcaagctgga tatatacgaa
      721 gacaaaagcc gcgacaattc gcccatttat aaattgccgg atcagccttg gttgggcgtg
      781 tctttcaatt taagcagcga gagcgtcgtc gaatcaaaga aactcaagaa agtggaatct
      841 tcttttagcg aggatgtgac gcagagcaat ggtgcgcaaa gcctatacaa atacaaaaac
      901 ctcgtttata cgacaggcga tgttcgtaat aagggtaatc aagttcatca ggacaaacac
      961 cacgctgtcg ccttttatct gaacgccaag ctgcacttgc tggataaaaa aaacatccaa
     1021 aatatcgcgc aaggcataac agttaatttt ggtaccttga aaacacgcat cgagccgacg
     1081 gacgcgtgga agaacaagag gcatctcact cataatgttg gtaattggga gttcaaagat
     1141 accggatcag tcagcgtcaa actcaaa
//
LOCUS       DAWFAN010000242         1112 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWFAN010000242 DAWFAN010000000
VERSION     DAWFAN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.242"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            153..1052
                     /locus_tag="WGO65_001971"
     CDS             153..1052
                     /locus_tag="WGO65_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0328213.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEIETVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
BASE COUNT          189 a          253 c          354 g          316 t
ORIGIN      
        1 gggttttatc tggcaggacg gttttttgcc ccaacggaaa atagcctgcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt caggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg tcagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gacggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgt gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtctt aatattgatt ccatcaaggc agtattggca
     1081 gttttccgtg caaaacctgc gttaccggac tg
//
LOCUS       DAWFAN010000243         1054 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWFAN010000243 DAWFAN010000000
VERSION     DAWFAN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.243"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(1..729)
                     /locus_tag="WGO65_001972"
                     /pseudo
     CDS             complement(1..729)
                     /locus_tag="WGO65_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694185.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          284 a          213 c          225 g          332 t
ORIGIN      
        1 tttattgaag atgttgttga gactagtctg tgatgtatta ctatgaggag tgagtacgga
       61 atcttctacc aatttattaa ttaaatcggg catcgtctgt ttgagcgtac taaattttgt
      121 tccaaactgt gctatatacg cctcctcagt ccgtttttta ttttctgccc aagcttccgg
      181 tcttgttttg tataaatcct gtaattgact tttaaccgcc tgctttaaat cagccccata
      241 cttctctgta atatattttt tgacaacggg catattatcg acggcgagga tgccgctatt
      301 gtaattttta ccttggatat tgatttcgtc atacccatat tcggatgaat tttgatcggt
      361 tttaccgctg gaaaaataat gggaagtgcc gggtttcggc ttgtctgaaa atgaaaaatc
      421 ggcatcgtcg ccgattttgg tagaacgagt atttgaacca agctgagctc tgaacatacc
      481 gaagcaatca aaagatatgc tgcacgggcc gcgccaaacg tgggaatttc tgtcttccgc
      541 cgcttctaaa ataaatttgt ttttaatctc ggcttctttg acgggatcgt acgcatccga
      601 acctcaaaca tatggcgtgc aaaacagcga taaaacagat aatgttaata gattaacccg
      661 accgcttgct tgcttgcttg cttgcttgct tgcttgcttg cttgcttgct tgcttgcttt
      721 cagtgtcatg agaaattttc ctttgtcaag tgttaaaatt ataatgatta tatactacat
      781 tatactacat cgcaatgaaa aatcaggaaa agcaaaaaac cctccgccgt cattcccgcg
      841 aaagcgggaa tctagaattt taatgccgca agaatttatc aaggatggtc ggggtttaaa
      901 ggtctggatt cccgcctgcg cgggaatgac ggtttagaaa tcacccgaaa caagaaaaag
      961 aaccgaaacc gaacaagccg gattcccgcc tgcgcgggaa tgacgggatt tcaggttact
     1021 gtttttgatt ttctgttttg tggaaataac ggga
//
LOCUS       DAWFAN010000244         1035 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWFAN010000244 DAWFAN010000000
VERSION     DAWFAN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.244"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(299..685)
                     /locus_tag="WGO65_001973"
     CDS             complement(299..685)
                     /locus_tag="WGO65_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ0328214.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(682..930)
                     /locus_tag="WGO65_001974"
     CDS             complement(682..930)
                     /locus_tag="WGO65_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ0328215.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
BASE COUNT          284 a          243 c          182 g          326 t
ORIGIN      
        1 cagccatata tattaagtac ctgctcttta tgcgcttttt taggaaagga agatcaaacg
       61 gttcaatagc gaagtcttgc ccaaatttga tggacaggat atagaataca tgcgtgcata
      121 ccacaccctg ccaaagttgg gagatgcact taaggttgca cttgtggtcg ccaactatgc
      181 ctatacacgc ggatttcccg cgtttatagg ccgcgacgga agtctggctt ttaagcgaag
      241 acaataaccg aaaagaccgc ctcggcggtc tttcttgtgt acacagtttt tatatttgtc
      301 agcttgatgc attgacaact ctaattccat attgcggaat atattcatcg acagtcatca
      361 gttcaaagcc ttccgcttgg gtttgtgcaa tcaacatcct atcgaaaggg tctttgtgta
      421 tttccggaag gcttccagcc tgttttgcat gaaacagacc tataggcagc atttcaaaat
      481 cctcttcttg aagcacatca aaaaactctt ccggtaattt caacaacccc ttgttctgct
      541 tgatggaaat ttcccaaata cttgctgcac tgacaaagat cgcatttctc ggattttcta
      601 tcagtttgcg tgcagatatc cccagtttct tgtcatccaa caaccaccac agcaaagcat
      661 gggtatcaag cagaatcttt ctcacagagc cgactcctca aaaaataaag ctgccgtttc
      721 attgtcatcc tcaagaatac gtgaaatatc cgtattttcc atatgactga attttttcaa
      781 ccttcctgca tttcgtgccg gtttttcaat accggttagt tggacgcaag gcttacctgc
      841 cttcgcaata ataacaattt cccctgcttc tgctctttga atcaattgac tcaaattggt
      901 ttttgcctga tgaatatttg cttgaaacat aacacttctc atgattagct aacttaacta
      961 atatacatca ttaccaagat tttgggaatc tcattacata tatttgatta tatccgccgt
     1021 tttattcaca ccttg
//
LOCUS       DAWFAN010000245          978 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWFAN010000245 DAWFAN010000000
VERSION     DAWFAN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.245"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            9..>978
                     /locus_tag="WGO65_001975"
     CDS             9..>978
                     /locus_tag="WGO65_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEZ0328216.1"
                     /translation="MLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLKNG
                     AAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTAFD
                     NSGDFVRLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTHID
                     DNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSVGVVGTPDKLAGESETVLKKF
                     VYECPMLNEILDKAVWENDFPFRSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSSGV
                     TIALHSAKLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFRTYSGLN"
BASE COUNT          230 a          290 c          253 g          205 t
ORIGIN      
        1 aagtctgcgt gttggaaaaa cagcactttc cgcgcttcgt catcggcgaa agcctgctgc
       61 cgcactgtat ggaaatgctg gaagaagccg gatttgccga tgccgtgcgc gccgagcccg
      121 gctttcagtt gaaaaacggc gcggcgtttt cgtggggcag ccgttatacc gaattcgatt
      181 tcaccgataa attttcagac ggccccggca cgacttatca agtgcgccgc gccgtgttcg
      241 ataaaatcct gattgaagaa gccgccaaac aaggcgttga agtacgtttc gggcacggcg
      301 taaccgcgtt cgacaacagc ggcgattttg tccgcctgaa catcgaaacc gacaccggca
      361 agcgctatga actgaccgcg aaattcgtct tggacgcaag cggttacgga cgcgtgctgc
      421 cgcgcctgct aaacttggaa acgccctcgc acctgccgcc gcgccaagcg catttcacgc
      481 acatcgacga caacatcacc aacccgaaat tcgaccgcaa caaaatcctg attaccacct
      541 atccgcaaca ccgcgacgtg tggatttggc tgattccctt cggcgacaac cgctgttccg
      601 tcggcgtggt cggcacgccc gacaaacttg ccggcgaatc ggaaacagtg ttgaaaaaat
      661 ttgtttacga atgcccgatg ctgaacgaaa ttttggacaa agccgtttgg gaaaacgatt
      721 ttccgttccg ctccatccaa ggctattccg ccaatgtgaa atcgctgcac ggcaggcatt
      781 tcgcgctatt gggcaatgcc gccgagttcc tcgatcccgt gttctcgtcg ggcgtaacca
      841 tcgcgctgca ctccgccaaa cttgccgccg atctgttggc aaaacaactc aaaggcgaag
      901 ccgccgattg gcaaaccgaa tttgccgaac ccttgatgat cggcgtggac gcgttccgca
      961 cctatagtgg attaaatt
//
LOCUS       DAWFAN010000246          970 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWFAN010000246 DAWFAN010000000
VERSION     DAWFAN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.246"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(53..619)
                     /locus_tag="WGO65_001976"
     CDS             complement(53..619)
                     /locus_tag="WGO65_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ0328217.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMLREGLSGGADDVREAKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAVIGMATTVMVTVGLTAGRALGVLF
                     GKRAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          268 a          300 c          211 g          191 t
ORIGIN      
        1 aaggcgaggc aacgacgtac tggtttttgt taatccacta tattttccga catcattgaa
       61 tcaaacccaa atgcgacaag agcgtccatg taccgatggc aatcaacacc aaacctccgg
      121 caaactcggc gcgtttgccg aacaatacgc ccaaagccct tcccgccgtc agcccgaccg
      181 taaccatcac cgtcgtcgcc atgccgatga cggcggcggc aaaggcgatg tttacctcca
      241 taaacgccaa gcccaccccg actatcatgg aatcaatact ggttccaaaa gcagtcagaa
      301 ccgtcaacca ccagctttcc cgtttggctt cgcgcacatc gtccgccccg ccggacaagc
      361 cctcacgcaa catcttcaga cccagcccgc ccagcaggac gaaagccgcc caatggtccc
      421 attcgctgat aaacggcttg gcataaaaac cgcccaccca gcctgccagc ggcgtgagcg
      481 cttcaaccgt gccgaacacc aaagccgttg ccgcaatttt gcgcggaggc attctgaccg
      541 ccgcaccctt tgccaatgcg acggcaaacg catccatcga catccccagc gcaatcaaga
      601 gcaaagcata aaaacccata ccgcacccgt cctcaaaaag ggcggattat agcaaaagca
      661 aaaaaatgca aaaacgccgc acgaaaaccc acatcccgtc attcccgcaa aaacaaaaaa
      721 tcaaaaactg aaatttcgtc attcccgcgc aggcgggaat ccagagttgt cggtgcggaa
      781 acttatcggg aaaaacggtt tctttagatt ttacgttcta gattcccact ttcgtgggaa
      841 tgacgggatg taggtttccg tgcggatgga ttcgtcattc ccgcgcaggt gggaatccaa
      901 agttgtcggt gcagaaactt attggataaa acggtttctt tagattttac gttctagatt
      961 cccgctttcg
//
LOCUS       DAWFAN010000247          933 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWFAN010000247 DAWFAN010000000
VERSION     DAWFAN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.247"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(112..744)
                     /gene="mtrR"
                     /locus_tag="WGO65_001977"
     CDS             complement(112..744)
                     /gene="mtrR"
                     /locus_tag="WGO65_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ0328218.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          209 a          205 c          243 g          276 t
ORIGIN      
        1 ctatgttatt gcaaaggtct caatatgtat ttttagaaga aggctttgtg tcattttgat
       61 gccgtctgaa agggtttgtt cgtttcagac ggcattgtta ccaaggcttg attatttccg
      121 gcgcaggtcg ggatggtttt ccaagttgtc catcattatc ccgatgatgc gcggggcggt
      181 tttgcccaaa tcgaaacgtt cgcaggaaga gaaccaccgc caaatcagcc cgtccaaggt
      241 tgatttgatg aagatgaccg ctgtttcctt gtccaaatcg tcagccaaat cctgattttc
      301 caccgcttcg gtcaaaacgg cggtaatttt ctcgcgccag attgcctgat gcttgcgggc
      361 aatggcgata acggcggcgt tttgttccgt gtgttcgcat tttaaaaaca ggatgttgtg
      421 gaatttgtag tagatgtcgt tgctttgcag ccgctcgaaa aagtgcagca gcgtgtggcg
      481 gaataccgcc caagaccctc cttcggcatc ttcggcatct tgcgcgatgc agttttcgat
      541 gtcgtcgcag atacgttgga acagcgcgtc gaacaagtct tccttatttt tgaaatgcca
      601 atagagcgcg ccgcgcgtta cgccggcggc ttgggcgatt tcgttgagcg aggtgcgcgc
      661 aatccctttg cggtaaaagg tttccaaggc ggcaagcatc aggtgttctt tggttttcaa
      721 ggcttcggtt ttggtttttc tcataatggc gttttcgttt cgggtcggtt tgatgagggc
      781 ggattataaa aaagactttg taaccatgca atcgtgtatg tataatgaaa cccatgaaat
      841 tgagactaca tctcaacttt gaaaacaggc ataacctgct ttgcaacccg ttcgaacatt
      901 cgggtatcaa agcaggcggt ttttatatcc tga
//
LOCUS       DAWFAN010000248          919 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWFAN010000248 DAWFAN010000000
VERSION     DAWFAN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.248"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..216
                     /locus_tag="WGO65_001978"
     CDS             <1..216
                     /locus_tag="WGO65_001978"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328219.1"
                     /translation="ADAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQG
                     NKPLGAEELADLKPLYKDLKNNIGNIV"
     gene            547..>919
                     /locus_tag="WGO65_001979"
     CDS             547..>919
                     /locus_tag="WGO65_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328220.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGL"
BASE COUNT          288 a          147 c          215 g          269 t
ORIGIN      
        1 gctgatgccg gagacgtata ttgcgtctat aacatcagac tgaagcagta cactgcctgc
       61 caggttaccc gagttgaaga acacggtggc aaaaaaacac atgcgaccct gctggctttg
      121 gactggcagg gcaacaaacc gcttggggcg gaggagctgg cggatttgaa accactttac
      181 aaagacttaa agaataatat tggaaatatt gtatgaacaa aaaattaaac tatattttta
      241 tgttggactg tttagggttg gtgatattgt ttacttgtat aatagctact tttgaaagag
      301 attatggatt taaaattttt actaattcta agaggcctga attttattat tggattggaa
      361 tgttttatta tggaattatt tcttgctggt ttgattatca attaatttca acaaaggcga
      421 attcgtataa aagaaaagtt aaacaatata aaattttttc agtaatattt tcagttttga
      481 tatttatttc tactatagta aaactttaaa ttttggagca aaaatttatg agcgattcaa
      541 ttgaatatgt attgggaacg cggtctgcac atgtataagg caagtgctgt cgtgccaacg
      601 ggatatgtac gggttgggaa taccgcgccg ctggtcggcg aagacacgca acggtatgcc
      661 tctttttggg gcgacggcta cgacgtgtac cgtcagttga gatggcagca gatacccgaa
      721 aaacagagaa aggcattcaa aaaagccgcc aaaagcaaaa agaccgtgat gtttgccgga
      781 cgggaatacg gcatatccaa acagaatttg agcgatgttt gggatgattt tgaagacgcg
      841 atggaactga aggcgtttcc ctgcctgtct tcgctgtttc tgaccaaatg gcataaaaat
      901 ctatatgata gtggattaa
//
LOCUS       DAWFAN010000249          914 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWFAN010000249 DAWFAN010000000
VERSION     DAWFAN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.249"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            313..873
                     /locus_tag="WGO65_001980"
     CDS             313..873
                     /locus_tag="WGO65_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ0328221.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          257 a          259 c          183 g          215 t
ORIGIN      
        1 ctataccacg atgaatcctt cgcaatatct gtttatcgac ctcaattttg acaaaatacc
       61 ggatacgcgc ctttgttgct tttccatctt ccaaccaact gtaaatctca aacagccggt
      121 acacgccatg cttcagtttc ttttcctgtc ggcggattgt ttcgacaaag aattgaaaat
      181 ccatttcatg caccttaaaa tttaatctgc attcaaacct tttcactttg gaagcaccat
      241 ttatcggatg tcccttcgca ataaacaaat tttcccgata tcgccgccca tttcaaccca
      301 aacccaaaag ctatgaaaaa cctcatcgcc ttcaacaaac cctatggcgt tatctgccaa
      361 ttttcaccgc acgaaaaaca caaaagcctc aaagacttta tcaatcttcc cggcttctac
      421 cccgccggac ggctcgacac cgacagcgag gggctgctgc tgctgaccga cgacggcagg
      481 cttcaggcac aaattaccga ccccaaattc aaacacccca aaacctactg ggcgcaactg
      541 gagggcgtac ccgacgaaag ccgattggaa agcctaagaa aagggataga cttaggcggt
      601 ttcgttaccc gtccggcaag catccgcatc ttgaaacacg gagaagcaga ttcgttatgg
      661 gagcgcatcc cgccgatacg cgtccgcaaa accgttcccg atttttggat tgaaattacc
      721 atttctgagg gcaaaaaccg ccaagtcagg cgaatgaccg ccaaggcggg ctatccctgc
      781 ctgcgtctga tcagagtggc aagcggcagg ctgaaactgt ttgatttgga tttaaaaccc
      841 ggggaatggg catacgcccc gtttaaacca taatcacgtt tatctcatca tttccacaaa
      901 agtgggaatc cgga
//
LOCUS       DAWFAN010000250          911 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWFAN010000250 DAWFAN010000000
VERSION     DAWFAN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.250"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(66..845)
                     /locus_tag="WGO65_001981"
     CDS             complement(66..845)
                     /locus_tag="WGO65_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ0328222.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          221 a          290 c          180 g          220 t
ORIGIN      
        1 gtactggttt aaatttagtt cactataccg atacatccga atatgccaga ccgtctaaac
       61 ggtattcata cctcccaatc ctgcacgcgc ttcaaatcgt tttgcgccaa agtatccgcg
      121 tggcggttac ggctctgata ttccctgtct ttcatgatgc tgcttgccac ataattcaaa
      181 tgtgcctttg ccgcctccga agcctcgccc ggccgacggt tcgatatcgc ctcatacagc
      241 acgcggtgct gcgccatcag tttcggacgc ggatcttctt cctgattcag ataaataagg
      301 ctgctgcgcg tctgccggta cagcattttc aacaaaccgc ccgacaaatg gctgaacaac
      361 aaattgtgcg ccgcatccgc aatcgtctga tggaagctga catcggcttc gctctgatgt
      421 tccagattgc cgctttcgca cgcctcctca aacttttcaa gccaaaaccc aatccgcttc
      481 aaatcggcat ccgtccggcg ttctgccgcc aatgccgcca tacagccctc gatgtggcag
      541 ctgaaatcaa aaacatcctg ttcccaattg gaatgcttgc ccaaaagctc ctgccaactt
      601 tgcaaaaaat cctgctgcgg cttgaccgaa acataataac cgtctccctg cctcgcttcc
      661 aaaacctgac gggcaaccaa aacattcaat gccgaccgga ccgacgggcg cgaaacgccg
      721 aactcttccg ccaaaacgcg ttcgggcgga atcttgcccc cttccgcgta aaccccttcc
      781 gcaatgcgct cctccaatac cgacaatacc tgatcgctga ttttctgagg ccttaccagt
      841 ttcattactc ctcctttata aagattccct gcagaaccct tccgaaatat agtggattaa
      901 caaaaatcag g
//
LOCUS       DAWFAN010000251          910 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWFAN010000251 DAWFAN010000000
VERSION     DAWFAN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.251"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            698..>910
                     /locus_tag="WGO65_001982"
     CDS             698..>910
                     /locus_tag="WGO65_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0328223.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     ILAEGQKSAVTEYYLNHGTWPGDNSNA"
BASE COUNT          306 a          253 c          150 g          201 t
ORIGIN      
        1 gaatcaactg ccggttgcat agaaacacca cctacggctt tctataaaaa tacctaaacc
       61 gtcattccca cgaacctaca tcccgtcatt cccgcgaaag tgggaatcca gaacgtaaaa
      121 tctaaagaaa ccgttttccc gataagtttc cgtgccgacg aggctggatt cccgcctgcg
      181 cgggaatgac gaagccatcc gcacggaaac ctgccgcgat gattcaactg ccgtttgcac
      241 aggaacacca cgcgccgatt tcaatcactt tccaagaaaa cggaaatttt taaaaattaa
      301 atttaaaatt tcccacccaa cccaccctat gctaccgcgc aaattcaaaa atctcaaatt
      361 ccgacccaat caacacaccc gatgccccat gccaatagaa ataccccacg ccaataaaaa
      421 agtgccgaaa atcggcacta aaactgacaa ttttcaacac tgatgacaaa aaacggtaac
      481 cccctgcttc ctcaaaccac gcccaaacga aagaaaatgt aaaataaaaa caaccatata
      541 aagataaacg cataaatttt cacctcaaaa cacaaacttg ataccaatct tgctttataa
      601 tacacaggta tcgcaacaaa ataccgatgg tcaaatacat tgcataatgc cgatggcgta
      661 agcttgaagc atttcctttc caattaggag taattttatg aacacccttc aaaaaggttt
      721 tacccttatc gagctgatga ttgtgattgc catcgtcggc attttggcgg cagtcgccct
      781 tcctgcttat caagactaca cagcccgcgc acaagtttcc gaagccatta ttttggccga
      841 aggtcaaaaa tcagccgtta ccgagtatta cctgaatcac ggcacatggc ccggcgacaa
      901 cagtaatgcc
//
LOCUS       DAWFAN010000252          873 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWFAN010000252 DAWFAN010000000
VERSION     DAWFAN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.252"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            134..>873
                     /locus_tag="WGO65_001983"
     CDS             134..>873
                     /locus_tag="WGO65_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEZ0328224.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYM"
BASE COUNT          233 a          202 c          233 g          205 t
ORIGIN      
        1 cgtctgaagc cttcgcgcct cccagatgcc gtctgaagta cgctccgcgc cttcagatgg
       61 catttgattt agttgaacgg gtaggatgaa ttggttaaac ttgcgggcaa tagtgccttc
      121 agacaaacaa aatatgccga acaaaaagaa aaaagccgcc tttgcgcgtg ctgcaacgga
      181 gaacggtgtg ccggacgtgt ccaacatcaa aaattcatat gatgatttga tgtatgaatc
      241 caaagcattt tcccaaaccg ccatcaacgc cttggaggct cgcgcccgca tgatgggttt
      301 gcagcccgcg cccgccgcca atgccaaggt cttggagctg ggctgttcga tgggcggcaa
      361 catcatcacg caggctcttt attacccgga tgcggaattt gtcggtatcg acttgtccgg
      421 caggcaggtt gcgcagggca acgccatcat tgaaaaaatg ggcttgaaaa atgtgcgtct
      481 ggaagaaaaa gatattttga ccgtcgatga gtcattcggg aagtttgact atatcatcgt
      541 ccacggcatt tggtcgtggg tgcctgacgc agttaaagac aaaatttttt cgatttgccg
      601 gaacaacttg accgaacacg gcattgccta tatttcatac aatgtttacc ccggctggaa
      661 acggcaggag cagttgcgcg aaattatgta ctttgccggc agggatgtgc ttgaagaacc
      721 cttggaagca cggacgcgga aaggcttgga cgcgctcaag gcgctggcgg aaattttaga
      781 aaacgacaag ggcttggacg gcggcggcaa acttccggcg attcaaaaaa tattgaatca
      841 taatttttat tacatcgcac acgaatatat gga
//
LOCUS       DAWFAN010000253          870 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWFAN010000253 DAWFAN010000000
VERSION     DAWFAN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.253"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            348..647
                     /locus_tag="WGO65_001984"
     CDS             348..647
                     /locus_tag="WGO65_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0328225.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFLFAVLTVLKLKGYQAGLYTLLIALAVAAFGFGMPAGMAVSSLPPQPH"
     gene            587..823
                     /locus_tag="WGO65_001985"
     CDS             587..823
                     /locus_tag="WGO65_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0328226.1"
                     /translation="MRLRDAGGYGGFFPAAAAALTQRPYATLDLTAHYKIGKSTRIGL
                     DFENVFNKRYRPMPDIHVYGTPRSLTATVKHIVD"
BASE COUNT          246 a          235 c          188 g          201 t
ORIGIN      
        1 atccactata catcaaacat caaattggac tgaccaatca gggcggattc taatgacacg
       61 ccgttcccgc cgtcaacggc atttacctcg caccgccccc gaaacacagg aaaaaactac
      121 accaaactac aatttttgtt tatgcaaata tttgttttga caggatttaa acgaaagctc
      181 cgattcaaat ctgccgaacc gcccaaaaat atatagtgga ttaacaaaaa tcaggacaag
      241 gcgacgaagc cgcagacagt acagatagta cggtaaggcg aggcaacgcc gtactggttt
      301 aaatttaatc cactctattg acctgaatat taaagtttcg caaagtaatg caacgttgct
      361 ttaattggtt tgaccactat tgccgacgat tagaaaaata ttttcggaga tgttcaatta
      421 tggaaacttg ggttcaaaac tacacggcaa tcggcggcag cctgtatctg actgccgccg
      481 ccgcactctt acccatcgtc tttttatttg ccgtgctgac cgtcctgaag ctgaaaggct
      541 atcaggcggg gctttatacg ctgctgattg cgcttgccgt tgccgcattc ggcttcggga
      601 tgccggcggg tatggcggtt tcttccctgc cgccgcagcc gcactgaccc aacgccccta
      661 cgccacgctg gatttgaccg cgcattacaa aatcggcaaa tccacccgca tcggtttgga
      721 ctttgaaaac gtgttcaaca aacgctaccg ccctatgccc gacattcacg tttacggcac
      781 gccgcgcagc ctgaccgcaa ccgtcaaaca tatagtggat taacaaaaat caggacaagg
      841 cgacgaagcc gcagacagta caaatagtac
//
LOCUS       DAWFAN010000254          828 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWFAN010000254 DAWFAN010000000
VERSION     DAWFAN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.254"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>828
                     /locus_tag="WGO65_001986"
     CDS             <1..>828
                     /locus_tag="WGO65_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328227.1"
                     /translation="RLEKFDRVALQHFSQYVDLINENNGRLPNTSEIERSYYKAVTEN
                     GVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNAQAVNNPNGSEWDNRKQLISA
                     LDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIGGWGNGIINDLYKSVAKRE
                     WTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKETKKRNGKYHVYDPLALDLD
                     GDGIETVATKGFAGSLFDHTNNGIRTATGWVSADDGLLVRDLNGNGIIDNGAELFGD"
BASE COUNT          262 a          143 c          194 g          229 t
ORIGIN      
        1 ccgtttagag aaatttgatc gggttgcatt gcaacatttc agccaatatg tagatctaat
       61 taatgaaaat aatggtagat tacctaacac tagtgaaatt gagagaagtt actataaagc
      121 cgttaccgaa aatggtgttt cttctagtgc agctattgat ttagttatta atcgctcact
      181 tccggatatg gcagatggtt attgggcatt aggtttgggg atagaagccg aacgtatcca
      241 caatgcgcaa gcagtaaata atccgaacgg tagcgaatgg gataatagaa agcagttaat
      301 atctgcttta gataaaggat ttgatggatc ttttaaagag aagcatttta cttttttaca
      361 atctgtgata atggatgtaa caaagttagg tgttgaatat acaatagatg gttggcaaaa
      421 aattggaggt tggggtaatg ggataatcaa tgatttatat aaaagtgttg caaaaagaga
      481 gtggactgga atatttgaga tcgttaataa taacatcaag caatttagag atctgttccc
      541 aaatccggaa ggctggatcg atgatggtca ccaatgtttc gctccttggg ttaaagaaac
      601 taaaaaacgc aatggcaaat atcatgtcta cgaccccctc gccctagatt tggacggaga
      661 cggtatagaa accgttgcca ccaaaggctt tgcaggcagc ttatttgatc acaccaacaa
      721 cggtatccgc accgccaccg gttgggtttc tgccgatgac ggtttactcg tccgcgattt
      781 gaacggcaac ggcatcatcg acaacggtgc ggaactcttc ggcgacaa
//
LOCUS       DAWFAN010000255          805 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWFAN010000255 DAWFAN010000000
VERSION     DAWFAN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.255"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..743)
                     /locus_tag="WGO65_001987"
     CDS             complement(join(<1..524,524..743))
                     /locus_tag="WGO65_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0328228.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDGQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHAK"
BASE COUNT          193 a          175 c          160 g          277 t
ORIGIN      
        1 cttagcatga ataatagagc aagccaagta aagcatcgat ttaaaattac gagctaattt
       61 ctcatagcga gtagcaatac tacgaaactg ctttaatcgt gcaaatgcat tctcgactaa
      121 gtggcgtaat ttataaaggt agctatcaaa atcagggtta ggtttcttgg catttttacg
      181 ctttggtata atagctttca taccgtgttc tatcgcttta tctctgattt cttgcgagtc
      241 ataccctttg tcagcgataa aatactgggc ttcttgtatg acttctatca agtcgtttgc
      301 aacttgaccg tcgtgcacgt tacccccagt gactttaaaa tcgagcggat ttccatgcga
      361 gtccacacat aggtgtattt ttgtcgtgtt tccgccacgg ctttgtccaa ttgctctatc
      421 gaaaccacgc cgagctccac ttgcatgttg atgacaccgt acataacttc cgtcgatgaa
      481 tacccattct ttgtcaattt cttttcgtag atcaaaaaaa aattctgcca caagcctttt
      541 ttagaccatc ggttaaagcg gttataagcg gttttccatg accctagctc aataggtatg
      601 tctcgccatg gtgcacctgt tcttagcttc catagtatgg cttccatcac ggtacggtcg
      661 ttcttccatt gatgacagcc gtgcgctttc atggttgttt gtaattgttc ccatatgttg
      721 tcagttattg cggttctcgc cattgtttgt gtttgcctat atttcgttgg aatatagggt
      781 taaaataggg ctcttttggg cttat
//
LOCUS       DAWFAN010000256          771 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWFAN010000256 DAWFAN010000000
VERSION     DAWFAN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.256"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..484
                     /locus_tag="WGO65_001988"
     CDS             <1..484
                     /locus_tag="WGO65_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0328229.1"
                     /translation="DKQIDDHTHTHFDGKAQVAEQIKGIGSITTATLMAMLPELRRLS
                     HKRIAGLAGIAPHPREGGETKFKSRCFGGRSAVRKALYMATVAATRFEPLIRDFYQRL
                     LSEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENDTAENDTAENDTAENGIRTA
                     "
BASE COUNT          190 a          190 c          222 g          169 t
ORIGIN      
        1 ggacaaacaa atcgacgacc acacccacac gcattttgac ggcaaagccc aagtggcgga
       61 gcaaatcaaa ggcatcggtt cgataacgac ggctacgctg atggcgatgc tgcccgaatt
      121 gaggcggctg tcgcacaaac ggatagcggg tttggccggc attgccccgc acccgaggga
      181 gggcggggaa accaaattta aaagccgctg ctttggcgga aggtctgcgg tgcgtaaggc
      241 actgtatatg gctaccgtgg cagcgacacg ttttgaaccg cttattcggg atttctacca
      301 acgcctgctg tccgagggta agccgtataa ggttgccgtt acggcatgta tgcgcaaact
      361 gctgacgata tcgaatgccc ggatgcgtga ttattttgcc gaaaacgata ccgccgaaaa
      421 cgataccgcc gaaaacgata ccgccgaaaa cgataccgcc gaaaacggta tccggacggc
      481 ttgatttgag ttttggtatt tttgcccgac ggggtgaaaa atacagttgc tacggctcga
      541 tgaatcgtca gaaatacccg aaccgtcatt cccgcgcagg tgggaatcca gaccggtcgg
      601 tgcggaaact tatcgggaaa aaaggtttct ccgtccctga atcctggatt cccactttcg
      661 tgggaatgac agaatgtagg ttcgtgggaa tgacgtggtg caggtttccg tgcggatgaa
      721 ttcatcattc ccgcgcaggc gggaatctag tctgttcggt ttcagttatt t
//
LOCUS       DAWFAN010000257          766 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWFAN010000257 DAWFAN010000000
VERSION     DAWFAN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.257"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>766
                     /locus_tag="WGO65_001989"
     CDS             join(<1..271,271..>766)
                     /locus_tag="WGO65_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ0328230.1"
                     /translation="EQCKNISQTAATFNLSRNTLYLWIRLKKQTGSLKHQVTGLNAVK
                     SDRQKPAQYIGQHQDAYLHEIAKHFDCTAATICYALKQMGITRKKRPTTYKEQDPAKV
                     THYLTQPAEFSDYQRVYLDETGFDRYLFRPYARSLKGQIVKAQISGKRYQRLSLVSAQ
                     VGNRLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKL
                     GHKVLPLAPYSPELNPIEKVWANIKRYLRTVLSDYAR"
BASE COUNT          236 a          181 c          167 g          182 t
ORIGIN      
        1 gaacaatgca aaaacatcag ccaaaccgca gcaacgttta acttgtcaag aaacacgctt
       61 tacctgtgga ttcgccttaa aaaacaaaca ggcagcctaa aacatcaagt taccggtcta
      121 aatgccgtca aatcggatag gcaaaaaccg gctcaatata ttgggcaaca ccaggatgcc
      181 tatctgcatg aaatcgccaa acattttgat tgtacggcag ccaccatttg ctatgcactc
      241 aaacagatgg ggataacgcg caaaaaaaga ccaccactta caaagaacaa gacccggcca
      301 aagtaacgca ttatttgaca cagccggccg aattttccga ctaccaacgt gtttatttgg
      361 atgaaacagg atttgaccgc tacctgttcc gtccctatgc ccgcagcctg aaagggcaaa
      421 tagtgaaagc gcagataagt ggaaaaagat accaacgctt atctttggtg tccgcacaag
      481 tcggcaaccg gctgattgct ccgatggttt atcaaaatac gatgaccgga gtcttttttg
      541 aagcgtggtt tcagcaatgc ctactgcccg cattgactca aaaatcggtg attattttag
      601 ataatgcgcg atttcaccgt atgggtgtct tacgggaaat ggcggaaaaa ttgggacata
      661 aggtattgcc tcttgcacct tattcacctg agctcaaccc gattgagaaa gtgtgggcga
      721 atattaagcg gtatctgcga accgttttgt ctgattacgc ccgatt
//
LOCUS       DAWFAN010000258          743 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWFAN010000258 DAWFAN010000000
VERSION     DAWFAN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.258"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            117..671
                     /locus_tag="WGO65_001990"
                     /pseudo
     CDS             117..671
                     /locus_tag="WGO65_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581383.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
BASE COUNT          225 a          200 c          142 g          176 t
ORIGIN      
        1 ttgttcatcc gctatattgt gttgaaacat cgccacaaac ctgatatagt ccgctcctac
       61 aacatcattg aaaatctttc ttttttaatc agttaaaacc gaatacggag ccgaaaatga
      121 atccagcccc caaaaaacct tctcttctct tctcttctct tctcttctct tctcttctct
      181 tctcttccgc agcgcaggcg gcaagtgaag acagcagccg cagcccgtat tatgtacagg
      241 cggatttagc ttatgccgcc gaacgcatta cccacgatta tccgaaagca accggtgcaa
      301 acaacacaag cacagtaagc gattatttca gaaacatccg tgcgcattcc atccaccccc
      361 gggtgtcggt cggctatgat ttcggcggct ggaggatagc ggcagattat gccagttaca
      421 gaaaatggaa caacaataaa tattccgtca acacaaaaga ggtgctaaga aacaataatg
      481 gcaacaggaa agaactgaag acggaaaatc aggaaaacgg tacattccac gccgcttctt
      541 ctctcggctt atccgccatt tacgatttca aactcaacga taaattcgat aaattcaaac
      601 cctatatcgg tgcgcgcgtc gcctacggac acgttaaaca tcaggttcat tcagtgaaaa
      661 aagaaaccac gactactttc cttgcaccaa cgcaaggcgc tacagtgcca ggcaagatcg
      721 tagaaggtcc gttcagcaaa cct
//
LOCUS       DAWFAN010000259          728 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWFAN010000259 DAWFAN010000000
VERSION     DAWFAN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.259"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            20..>728
                     /gene="rpe"
                     /locus_tag="WGO65_001991"
     CDS             20..>728
                     /gene="rpe"
                     /locus_tag="WGO65_001991"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ0328231.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEI"
BASE COUNT          178 a          172 c          210 g          168 t
ORIGIN      
        1 gatattgaag gaaaaaaaca tgactactta ccgtatcgca cccagcattt tatcggcaga
       61 ttttgcacgg cttggcgaag aggtggaaag cgtcattgcg gcaggtgcag atctgatcca
      121 ttttgacgtg atggacaacc attatgtgcc gaacctgacc ttcggcccta tggtttgcgc
      181 ggcgttgaag ccttatgcaa gcgtgccgat cgatgtgcat ctgatggtcg aacccgtgga
      241 tgacctgatt cagtcgtttg ccaaagcagg agcatcaatc atcacgttcc atcccgaagc
      301 gagccgccat attgaccgca gcttgagcct gattcgggac atgggctgtc aggcggggct
      361 ggtgttgaat ccggcaacgc ccgtatatct gttggaaaac gtattggaca ggctggatat
      421 ggttttgctg atgtcggtaa accccgggtt cggcggtcag agcttcattc cgtatacttt
      481 ggaaaaaatc agacaggttc gtgccatgct tgaccgctat gaagagaaaa gcggcagacg
      541 tattgccatt gaagtggacg gcggcatcaa aaccgacaat atcgccgccg ccgcccaagc
      601 cggcgcggat acctttgttg ccggttctgc gattttcggc aaaccagact ataaggcggt
      661 gattgatgcg atgagggcgg aactggaaaa aagcggcagg ctgaaccttg cgccaatgat
      721 tgaaatag
//
LOCUS       DAWFAN010000260          718 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWFAN010000260 DAWFAN010000000
VERSION     DAWFAN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.260"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..265
                     /locus_tag="WGO65_001992"
     CDS             <1..265
                     /locus_tag="WGO65_001992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0328232.1"
                     /translation="RDGTAGTNNDEVKAATGTTANIDTKHLPSTCRDDSSTGCIETPR
                     ADFKHFQKISRYRVLPESRHMAEKLRHSRAGGNPGLSVRKLIG"
     gene            312..>718
                     /locus_tag="WGO65_001993"
     CDS             312..>718
                     /locus_tag="WGO65_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0328233.1"
                     /translation="MTGFNDAAGMASSPSDIKGKYVQSVTVANGVITAQMASSNVNKE
                     IKDKKLSLWAKRQDGSVKWFCGQPVQRANANGANNDEVTAATGKGTDKIDTKHLPSTC
                     RDASSAVCTKTPEYYLNHGKWPDGNSNAGVASSS"
BASE COUNT          200 a          229 c          166 g          123 t
ORIGIN      
        1 gcgcgacggc accgccggca ccaacaacga cgaagtcaaa gccgccaccg gcaccaccgc
       61 caacatcgac accaagcacc tgccgtcaac ctgccgcgat gattcatcta ccggttgcat
      121 agaaacacca cgcgccgact tcaaacactt ccaaaaaatc agccgttacc gagtattgcc
      181 cgaatcacgg cacatggccg aaaaacttcg tcattcccgc gcaggcggga atccaggtct
      241 gtcggtacgg aaacttatcg ggtaaaacgg tttcttgaga ttttgcgttc tggattccca
      301 ctttcgtggg aatgacggga tttaatgatg ccgccggcat ggcatcctcc ccctccgaca
      361 tcaaaggcaa atatgttcaa agcgttacag tcgcaaacgg cgtcattacc gcccaaatgg
      421 cttcaagcaa cgtaaacaaa gaaatcaaag acaaaaaact ctccctgtgg gccaagcgtc
      481 aagacggttc ggtaaaatgg ttctgcggac agccggttca gcgcgccaac gccaacggcg
      541 ccaacaacga cgaagtcacc gccgccaccg gcaagggcac cgacaaaatc gacaccaagc
      601 acctgccgtc aacctgccgc gacgcatcat ctgccgtttg cacaaaaaca cccgagtatt
      661 acctgaatca cggcaaatgg cctgacggca acagtaatgc cggcgtggca tcttcttc
//
LOCUS       DAWFAN010000261          714 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWFAN010000261 DAWFAN010000000
VERSION     DAWFAN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.261"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(102..497)
                     /locus_tag="WGO65_001994"
     CDS             complement(102..497)
                     /locus_tag="WGO65_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328234.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSYLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKFAKVSG"
     gene            complement(481..>714)
                     /locus_tag="WGO65_001995"
     CDS             complement(481..>714)
                     /locus_tag="WGO65_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0328235.1"
                     /translation="VIAQSEFVEFAKINKSENVIDCFSFFWNRRIKHDIWLISFSDNS
                     EMVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
BASE COUNT          196 a          158 c          130 g          230 t
ORIGIN      
        1 ctccataaca cctcctaatt ttacccaaat acccccttaa tcctccccgg atacccgata
       61 atcaggcatc cgggttacct tttaggcggc aacaggcgca cttagcctga gacctttgca
      121 aatttgtcgg tttcggtgtc gtattggtag cctcgtgcct gtatgtcttc tttgaaagtt
      181 tcgtatacgt catgggctaa aagggctgtt ccgacatagg gaaccgccct tgtgctgaat
      241 ttcgcgccta agcgggcaag tttgccgccc cccgccaata cgccggcgcg ggaaatgctg
      301 gcggttattt tggcgttgat tcgggctttt gcgcccgtag ggatgtgtgt taaatctacc
      361 gtttttatta aataagatga ataagtttta ctatttttag gtacaaactt atgaattttc
      421 gcaccttgtc cggtatcaac tgaaacagtt tcagatattt ttactgcatt tgcattcgct
      481 tcaaacgaat acatcatcaa aattgcaatt atcgacaatt tcgcaaaatt caaatttgta
      541 tattttatga ccatctttca gggattcttt aattaccatt tctgaattat cagaaaatga
      601 gattagccaa atatcatgtt taattcttct attccagaaa aaagagaaac aatcaataac
      661 attttcagac ttattaatct tcgcaaattc aacaaattca gattgcgcta taac
//
LOCUS       DAWFAN010000262          694 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWFAN010000262 DAWFAN010000000
VERSION     DAWFAN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.262"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            180..509
                     /locus_tag="WGO65_001996"
     CDS             180..509
                     /locus_tag="WGO65_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0328236.1"
                     /translation="MGSLIIEDLQEGFGKEAVKGKEITVHYTGWLEDGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            538..694
                     /locus_tag="WGO65_001997"
                     /pseudo
     CDS             538..694
                     /locus_tag="WGO65_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          208 a          185 c          178 g          123 t
ORIGIN      
        1 aaatttaatc cactatattt ccatgaaaaa gcatggaacc tttacgaaaa aaataaaaca
       61 gtcaaacaaa cacagccgtt tcagctgcac cggagcagct gacgtttgag cggcatttga
      121 cttcgccgga cggtatgtta cccttgccgt tttaaccaac aagcaacgaa aggacaaata
      181 tgggcagcct gattattgaa gatttgcagg aaggcttcgg aaaagaagca gttaaaggca
      241 aagagattac cgtgcattac acaggttggc tggaagacgg caccaaattc gactccagcc
      301 tcgaccgccg ccagccgctg accatcacgc tcggtgtcgg acaagtcatc aaaggctggg
      361 acgaaggctt cggcggaatg aaggaaggcg gcaaacgcaa gctgaccatc ccttcggaaa
      421 tgggctacgg cgcacacggc gcgggcggcg tgattccccc gcacgccact ttgatatttg
      481 aagtcgagct gctgaaagtg tacgaataaa gctgcccggg caataccgtc tgacataatg
      541 gcttcaagca acgtaaacaa agaaatcaaa gacaaaaaac tctccctgtg ggccaagcgt
      601 caagacggtt cggtaaaatg gttctgcgga cagccggtta cgcgcaccac cgccggcgcc
      661 aaagacgaca ccgtcgccgc cgacaccgac accg
//
LOCUS       DAWFAN010000263          656 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWFAN010000263 DAWFAN010000000
VERSION     DAWFAN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.263"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(53..538)
                     /gene="fxsA"
                     /locus_tag="WGO65_001998"
     CDS             complement(53..538)
                     /gene="fxsA"
                     /locus_tag="WGO65_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ0328237.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          196 a          212 c          116 g          132 t
ORIGIN      
        1 tttcaaaccg gaaaaatgcc gtctgaatct tcagacggca ttccattcat atttattcgt
       61 cttttttgtg ttcgatggcg tttcgggaac gattgccgcc gtaaggctct tcaaccgtat
      121 attccccctc gataatatcg tcatcgcggg aaaagccctc ttttctgccc gattggttca
      181 tgttgaaaaa attttccgca cctcctgcct gcaacactgc acctccctta aacggcagca
      241 gcagcaatac cgccaacacc gaggatacga atcccggact catcagacac accgccgcca
      301 ccgtataacg gataggccac aacatctgat aaacggatac cctcccgccg cttctcattg
      361 ccgcgcccgc caataaaaga ccggacagcc ccgtatgcct gagcatcagc acaccggcgg
      421 caaaacctgc cgccatcaaa aacaacgtcc agccgccgcc caaccaatcg gcaacccaca
      481 caatcgacat aatctccaaa aacagcagca ccaaaaaacc gataccgaaa aatctcattg
      541 accgtcatcc ttatatttaa gtaaacagca aaccgcccga acaagactcc aagcgacctg
      601 cctgtaaatg attacaaaac catgtgcttc aagccgaaac aatgtgaaat cacgca
//
LOCUS       DAWFAN010000264          653 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWFAN010000264 DAWFAN010000000
VERSION     DAWFAN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.264"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            227..520
                     /locus_tag="WGO65_001999"
     CDS             227..520
                     /locus_tag="WGO65_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328238.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPDCFDMKAVWQMFNRIPR"
BASE COUNT          166 a          164 c          170 g          153 t
ORIGIN      
        1 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta tattgtccat
       61 tcggcaggga aagcctgaag aagaattttc ccgcaagatg gccggctgcc taaccgactt
      121 ggaaaactgt ttggaaaaat acctcgaaca gttcgggccg gtatcggaaa gtatcgaagc
      181 ctgtaccgtc aaactgcagg agtaaccgtc gttcttcaac aggctgatga aaaccaacga
      241 caagctcaat aggcagattg acgtgttgca gaaacagtcg gcagccatcc acaacgaagc
      301 ctatatcgaa atgaacacgc tgctttaccg ccaccgcgaa gtggtttccg tccacaaccg
      361 caaggcggat tatgcggaaa agggcaaaga gcggattgcg ctgttcccgc gcggcttgaa
      421 cggcatcacc aagctgcctg ccgccgtcct gttgcccgag cgtcccgatt gttttgatat
      481 gaaggcagtt tggcaaatgt ttaatagaat accgaggtag gcgtatcggc tgccggataa
      541 aaatgccgtc tgaaacggaa gcgttcagac ggcattgttg cagtggaaca aatcaaaatg
      601 caccccgtca ttcctgtctg tacggtgata gcggcagtgt tttcctgctt cgt
//
LOCUS       DAWFAN010000265          636 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWFAN010000265 DAWFAN010000000
VERSION     DAWFAN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.265"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..584)
                     /locus_tag="WGO65_002000"
     CDS             complement(<1..584)
                     /locus_tag="WGO65_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0328239.1"
                     /translation="MIASRLSGNISSAIQEHKDGKVNINRFQNILADLYSLGGLGSTL
                     IEKNGNMQSWGIPLAIAGDIIAATAIATGDTGTISTEEFYNFDNWKGFGYELFEDWSR
                     WVYDWLPDGWNLWKELDRNRSGQYHIYDPLALDLDGDGIETVATKGFSGALFDHRNQG
                     IRTATGWVSADDGLLVRDLNGNGIIDNGAELFGD"
BASE COUNT          160 a          160 c          136 g          180 t
ORIGIN      
        1 ttgtcgccga agagttccgc accgttgtcg atgatgccgt tgccgttcaa atcgcgcaca
       61 agcagaccgt catcggcaga aacccaaccg gtggcggtgc ggatgccttg gttgcggtgg
      121 tcgaacaatg cacctgaaaa gcctttggtg gcgactgttt ctatgccgtc gccgtctagg
      181 tctagggcga gggggtcgta gatgtggtat tggcctgaac ggtttctgtc caattctttc
      241 cacagattcc agccgtcggg cagccagtcg tatacccaac gagaccagtc ttcaaatagc
      301 tcatacccaa aacctttcca gttgtcaaaa ttataaaatt cctctgtaga tatcgtacca
      361 gtatctcctg tggcaatagc cgttgctgca attatatctc cagcaattgc taatggaatc
      421 ccccaactct gcatatttcc attcttctct attaatgtac ttcctaaccc tcccaatgaa
      481 tataaatccg ctaaaatatt ttgaaaacgg ttgatattaa ctttaccatc tttatgctcc
      541 tgaatagctg aagatatatt tccagagaga cggcttgcaa tcacagaggc atttgttccg
      601 tttacaatat gcgatgacgt actacctggt gcaata
//
LOCUS       DAWFAN010000266          627 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWFAN010000266 DAWFAN010000000
VERSION     DAWFAN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.266"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>627
                     /locus_tag="WGO65_002001"
     CDS             <1..>627
                     /locus_tag="WGO65_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0328240.1"
                     /translation="RSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQID
                     NAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAA
                     DGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDD
                     CPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPI"
BASE COUNT          167 a          185 c          150 g          125 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggtggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgtccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggtgccgc acaaatggac ggtgcaatct tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gccaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatc gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 gaaaatcttc gaattggctg ctgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaacct ttcttgttgc ctatcga
//
LOCUS       DAWFAN010000267          607 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWFAN010000267 DAWFAN010000000
VERSION     DAWFAN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.267"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            61..444
                     /locus_tag="WGO65_002002"
     CDS             61..444
                     /locus_tag="WGO65_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ0328241.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
BASE COUNT          148 a          145 c          155 g          159 t
ORIGIN      
        1 ggcaacgccg tacaggtttt tgttaatcca ctatattgag aacattttga aaggataccg
       61 atggaacctt cctcctacgc ggcagaaaaa aaaggcaaag gcggcatcag gcgcgtcatt
      121 aacgcattcg gctattcgat agacggcatc gccgccgcct accgttacga agcggcattc
      181 cgtcaggttt tgtggctgaa cgcgctgctg gtgtgtgcgg catttttttg ggtttccgaa
      241 accgccgtcc gcctgccgtt gattatcgcg tcttttgtgt cggtcattgt cgaactgttc
      301 aacaccgccg tcgaagctgc cgtcgatcat acttcgaccg aaaaacacga gctggccaaa
      361 cgcgccaaag acgcaggttc tgccgcacaa ttgttcgcga tgctgatgtt ggcggcggtt
      421 tggctgtctg ccctgttcgg gtaaaacgct tgcagcagga tttgtaatcc tttaggatta
      481 gtatgtggta tttattattc acttaatcta catcaaattt ccaggcagta tttgtatgta
      541 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg
      601 aaccgat
//
LOCUS       DAWFAN010000268          601 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWFAN010000268 DAWFAN010000000
VERSION     DAWFAN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.268"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            92..467
                     /locus_tag="WGO65_002003"
                     /pseudo
     CDS             92..467
                     /locus_tag="WGO65_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            504..601
                     /locus_tag="WGO65_002004"
                     /pseudo
     CDS             504..601
                     /locus_tag="WGO65_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          151 a          132 c          159 g          159 t
ORIGIN      
        1 tggcggcact tactttttta cggttgtagc caataaacgg cagaagattt tgaccgatga
       61 tgcggtgcgt ttggctttac ggcaggcggt aatggcggtg cgcgaacggt atccgtttga
      121 aattttggca tgggtgttga atgcccgacc atctgcatac catatggcgg ctgccggaca
      181 atgattctgc ttattcggaa cgctggcggc agatcaagcg gcacagcaaa tatttaatcg
      241 gcggcaatct caggctttgg caaaaacgct tttgggaaca tactatccgc gatgaggccg
      301 attttgcctg ccattttgat tatctgcatt tcaatccggt caaacatggc tatgtaggac
      361 aaatttccga ttggcggttt tctacgtttc accgttatgt caaacagggt atttatccgc
      421 ataattgggg tggcggcaat gcggactttt ctattgaata cgattaaagt aatgtcggat
      481 tcaagaatcc gacctacgga aaactgaaag agcatcggct gcaggacggc attatgcgca
      541 aagcctgccg caaccgtccg ctggcggaag tgcaaaccaa acgtaaccga tatttgtcga
      601 a
//
LOCUS       DAWFAN010000269          593 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWFAN010000269 DAWFAN010000000
VERSION     DAWFAN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.269"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..548
                     /locus_tag="WGO65_002005"
     CDS             <1..548
                     /locus_tag="WGO65_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0328242.1"
                     /translation="ADMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDNHT
                     HTHFDGKDQVAEQIKGIGSITTATLMAMLPELGRLLHKRIAGLAGIAPHPREGGETKF
                     KSRCFGGRSAVRKALYMATVAATRFEPLIRDFHQRPLSEGKPYKVAVTACMRKLLTIS
                     NARMRDYFAENDTAENGIRTA"
BASE COUNT          152 a          145 c          172 g          124 t
ORIGIN      
        1 cggcggatat gcggactgcc gagaaaaacc gtctgcatca ggttcacgaa acgcaagtcg
       61 gaagcgtcaa acaactgatt gcccattttg accggctgat tgacgaattg gacaaacaaa
      121 tcgacaacca cacccacacg cattttgacg gcaaagacca agtggcggag caaatcaaag
      181 gcatcggttc gataacgacg gctacgctga tggcgatgtt gcccgaattg gggcggctgt
      241 tgcacaaacg gatagcgggt ttggccggca ttgccccgca cccgagggag ggcggggaaa
      301 ccaaattcaa aagccgctgc tttggcggaa ggtctgcggt gcgtaaagca ctgtatatgg
      361 ctaccgtggc agcgacacgt tttgaaccgc ttattcggga tttccaccaa cgcccgctgt
      421 ccgagggtaa gccgtataag gttgccgtta cggcatgtat gcgcaaactg ctgacgatat
      481 cgaatgcccg gatgcgtgat tattttgccg aaaacgatac cgccgaaaac ggtatccgga
      541 cggcttgatt tgagttttgg tatttttgcc cgacggggtg aaaaatacag ttg
//
LOCUS       DAWFAN010000270          589 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWFAN010000270 DAWFAN010000000
VERSION     DAWFAN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.270"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(104..388)
                     /locus_tag="WGO65_002006"
     CDS             complement(104..388)
                     /locus_tag="WGO65_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0328243.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          174 a          159 c           96 g          160 t
ORIGIN      
        1 gattcccgcc tgcgcgggaa tgacggttta gaagttgccc gaaacctcaa aaaaaaaacc
       61 gaaaccgtac aagccggatt cccgcctgcg cgggaatgac gggctaaata atatcaaacc
      121 ataaatcctg ccaagaaaca ttattttctt caatcagttg caatttccaa gccctgttcc
      181 atttcttcaa ctgtttttcc cgagtaattg cactctccat cgtaggatgc agttcatacc
      241 aaaccagcat agtaacgttg taccgtgatg taaatccctc aatcaaatgc tccctatgtt
      301 ggtaaatacg ttgcaccaaa tcagatgtaa cgccaatgta taacgtgcca ttacgttggc
      361 ttgctaaaat ataaaccgca ggctgcatat aatacccttt tgaattattt caatttatat
      421 tcccgcgaac accatcccgt gattacttta acccttcgtt attcccatag ctttccatca
      481 ttcccgcaac tcttcgtcat tcccgcgaaa gtgggaatct agaacgcaaa atctaaagaa
      541 accgttttac ccgataagtt tccgcaccga caaacctaga ttcccgcct
//
LOCUS       DAWFAN010000271          585 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWFAN010000271 DAWFAN010000000
VERSION     DAWFAN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.271"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..329)
                     /locus_tag="WGO65_002007"
     rRNA            complement(<1..329)
                     /locus_tag="WGO65_002007"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          123 a          153 c          104 g          205 t
ORIGIN      
        1 tatcacccac tatggtcaag cttcccagct tgttcttcta tctcgacagt tatcacgtac
       61 aggctcctcc gcgttcgctc gccactactt gcggaatctc ggttgatttc ttttcctccg
      121 ggtacttaga tggttcagtt ctccgggttc gcttctctaa gtctatgtat tcaacttagg
      181 atactgcaca gaatgcagtg ggtttcccca ttcggacatc gcgggatcat agctttattg
      241 ccagctcccc cgcgcttttc gcaggcttac acgtccttcg tcgcctatca tcgccaaggc
      301 atccacctga tgcacttatt cacttgactc tatcatttca agaacttctt tgacttcgtt
      361 tacctacccg ttgactaggg aagtaaactt gaaattccta ctttgataaa gcttactgct
      421 ttgttgtgtc ttaatcctgc cttttgtgtt tcaggattaa gtcgatacaa tcatcaccca
      481 aatactgtgt ttgttttctt ttctctcgcg agagattttt accctttgca aaaggtaaaa
      541 atcaaaacaa actcattgtc tttgtttgtt gatttcggct ttcca
//
LOCUS       DAWFAN010000272          555 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWFAN010000272 DAWFAN010000000
VERSION     DAWFAN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.272"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT          184 a           98 c           77 g          196 t
ORIGIN      
        1 tagaaattag ttaaagaata tattatggta gatactaact ctttagttaa aaataattta
       61 ctaactttta ctacttaggt ataaattcca atacttaact aaactgaggg taaatatatt
      121 tatccaagat agaagagaaa cttttttctc tgtttccgca agaatagtta cgggttgtga
      181 aatatgctct atacccaagc agtagatatt ttcattgtta cttaccgtat tgcttcatag
      241 aattggcact ctacgctttc ctttaaaaat cgtcaataac gattatcatt ttctttaaat
      301 ttattcaaaa caatattctt atatacatct caagcccgct gaaataaagc aaaatggtgg
      361 ttcatcgact gtaatgccaa tttcaaagca gcctgcactt tgacaacaca caagatatac
      421 gggaaggaca gggcatcagc atatatttca gcccgaagcc gtttcattcg tttaaaaaac
      481 tgcttttttc acaatttacc tttacaaagc ctatacgtat ccttacaatt tgttttgctg
      541 tttttggtat atttt
//
LOCUS       DAWFAN010000273          548 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWFAN010000273 DAWFAN010000000
VERSION     DAWFAN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.273"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT          159 a          152 c          115 g          122 t
ORIGIN      
        1 taaagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca
       61 ctatatccgt ttgcgcccgc caagccgtgt tgcacagatg cgacacggct tttttctttc
      121 tgttgtcaga atgtaaattt tcgtcaaaaa acatcaaaaa tcatcccccc cccgaatttg
      181 cgtaaaataa agtgtaatcc ctttgatcat aagggaatgt tgacaattag atcgattctc
      241 ctaggctgac tttcgcaata aatggggttg tcaataattg tgtatggtcg tttgccgtcc
      301 ggcggccgtc attcccgcgc aggcgggaat ccagaaacgc aacgcagcag gactttatcg
      361 gaaaaaaccg aaatcccacc gccgtcattc ccgcgcaggc gggaatccag caacacaacg
      421 cagcaggact ttatcggaaa aaaccgaaac cccaccgacc gtcattcccg caaaagcggg
      481 aatccagcaa ccgaaaaacc acaggaatct atcagcaaaa acagaaaccc caccgccgtc
      541 attcccgc
//
LOCUS       DAWFAN010000274          548 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWFAN010000274 DAWFAN010000000
VERSION     DAWFAN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.274"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..378
                     /locus_tag="WGO65_002008"
     CDS             <1..378
                     /locus_tag="WGO65_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0328244.1"
                     /translation="LERLAARDIIRFPGFVNNVSEVIKAHHIFVLPSYREGVPRSTQE
                     AMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKMIYFIENREAVRLMGNAS
                     YAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          131 a          134 c          137 g          146 t
ORIGIN      
        1 ttggaacggc ttgccgcccg cgatattatc cgtttccccg gttttgtgaa caatgtttcc
       61 gaagtgataa aggcgcatca tatattcgta ttgccgtctt atagggaagg cgttccccga
      121 agcactcagg aggcaatggc cgtcggcagg gcggtgatta cgacggatgt ccccggatgc
      181 agggaaacgg tcgccgacaa ggtcaacggc ttcctgatcg aaccttggaa tccccgcatc
      241 ttggccgaaa aaatgattta ttttatcgaa aacagggaag ccgtccgcct gatggggaat
      301 gcaagttatg cgattgccaa agataaattc gatgccgaaa aagtcgattt gaaattgctc
      361 gatattttga aggcgtaaac aaggctgccc gcttttgagt ttcggtcatt tctgataaac
      421 ccccgtcatt cccgccacac ccccgtcatt ccctcgaaag cgggaattca ggttcgttta
      481 gtttcggtca tttccgataa attcttgcaa ctttgcgttt ctagattccc actttcgtgg
      541 gaatgacg
//
LOCUS       DAWFAN010000275          536 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWFAN010000275 DAWFAN010000000
VERSION     DAWFAN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.275"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>536
                     /locus_tag="WGO65_002009"
     CDS             <1..>536
                     /locus_tag="WGO65_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0328245.1"
                     /translation="PELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRNWLAQDDTLS
                     ELLKLINRQLTEKGLKVEKASAAVVDATIIQTAGSKQHQAIEVDNKGQVSGQTTPSKD
                     SDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLSPLLEGLPEGTTVY
                     ADKGYDSAENRQHLEEHQ"
BASE COUNT          166 a          163 c          118 g           89 t
ORIGIN      
        1 atcccgaact cgaacacagc ctcatcaccc gcatcgactt caacctgttt tgccgtttcg
       61 acgaactgag catccctgat tacagcacct tatgccgcta ccgcaactgg ctggcgcaag
      121 acgacaccct gtccgaattg ctcaaactga tcaaccgaca actgaccgaa aaaggcctaa
      181 aagtagagaa agcatccgcc gccgtcgttg acgccaccat tattcagacc gccggcagca
      241 aacagcacca ggctatagaa gtcgataaca aaggacaagt cagcggccaa accacaccga
      301 gtaaggacag cgatgcccgt tggatcaaga aaaacggcct ctacaaactc ggttacaaac
      361 aacatacccg taccgatgca gaaggctata tcgagaaact gcacattact cccgccaatg
      421 cccatgagtg caaacacctg tcgccgttgt tggaagggtt acccgaaggt acgaccgtct
      481 atgccgacaa aggctacgac agtgcggaaa accggcaaca tctggaagaa catcag
//
LOCUS       DAWFAN010000276          531 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWFAN010000276 DAWFAN010000000
VERSION     DAWFAN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.276"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>531
                     /locus_tag="WGO65_002010"
     CDS             <1..>531
                     /locus_tag="WGO65_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0328246.1"
                     /translation="TEKGLKVEKASAAVVDATIIQTAGSKQRQAIEVDNKGQVSGQTT
                     PSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLPPLLEGIAEG
                     TTVYADKGYDSAENRQHLKEHQLLDGIMRKACRNRPRTETQTKRNRYLSKTRYVVEQS
                     FGTLHRKFRYARAAYFG"
BASE COUNT          163 a          147 c          132 g           89 t
ORIGIN      
        1 gaccgaaaaa ggcttaaaag tagagaaagc atccgccgct gtcgttgacg ccaccattat
       61 tcagaccgcc ggcagcaaac agcgtcaggc tatagaagtc gataacaaag gacaagtcag
      121 cggccaaacc acaccgagta aggacagcga tgcccgttgg atcaagaaaa acggcctcta
      181 caaactcggt tacaaacaac atacccgtac cgatgcagaa ggctatatcg agaaactgca
      241 cattaccccc gccaatgccc atgagtgcaa acacctgccg cctttgctgg aagggatagc
      301 cgaaggtacg accgtctatg ccgataaagg ctatgacagt gcggaaaacc ggcaacatct
      361 gaaagaacat cagttgttgg acggcattat gcgcaaagcc tgccgcaacc gtccgcggac
      421 ggaaacgcaa accaaacgca accggtattt gtcgaagacc cgttatgtgg tcgaacaaag
      481 cttcggtacg ctgcaccgta aattccgcta tgcgcgggca gcctatttcg g
//
LOCUS       DAWFAN010000277          508 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWFAN010000277 DAWFAN010000000
VERSION     DAWFAN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.277"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            1..111
                     /locus_tag="WGO65_002011"
                     /pseudo
     CDS             1..111
                     /locus_tag="WGO65_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            226..379
                     /locus_tag="WGO65_002012"
                     /pseudo
     CDS             226..379
                     /locus_tag="WGO65_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271703.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
BASE COUNT          140 a           92 c          149 g          127 t
ORIGIN      
        1 aaactgatta accgccaact gaccgaaaaa aacctaaaag tagagaaagc atccgctgcc
       61 gtcgttgacg ccaccattat tcaggccgcc ggcagcaaac agcgccaggc ccgataacgc
      121 ccggcggaag tttttcggac atcgcgaacc ttgccctgct gaaaatacaa aaggccgcgt
      181 tcggcgatat ggggtgggaa caggtgagga gggtggatac ggggcatggt tggcttccaa
      241 gaagttgttg aaatatgggg agtttactgg ttcgggcgac gtttggggag aggggtggga
      301 attcttgttt tgcgaagtgg aaaaaggtcg tctgaaaatg ggtttcagac gacctttttt
      361 agggtggaaa ggggagtagt ttagaaagtc cacacatatt ctgcgtatgg attccaagtt
      421 gtatagggtg gtatcttcca aaaatacata tgagtaggat agtgttatga gttgacaact
      481 tgtttattga aaatatcttt cttgaact
//
LOCUS       DAWFAN010000278          507 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWFAN010000278 DAWFAN010000000
VERSION     DAWFAN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.278"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(323..398)
                     /locus_tag="WGO65_002013"
     tRNA            complement(323..398)
                     /locus_tag="WGO65_002013"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Glu,seq:ttc)
BASE COUNT          139 a          122 c          133 g          113 t
ORIGIN      
        1 aatactttga tgctttggtg ggctgaagcc caccctacaa tatagagaaa atgccgccaa
       61 acggtatgga agcggaaaca atggtggggc tggcttcaga aacaatggta gggtgggctt
      121 cagcccaccg ctttagacgg catccgcgca ccgtcattcc cgcgcaggcg ggaatccagc
      181 cttgtcggca cggaaactta tcggataaaa cggtttcttc aaccctacgc tctagattcc
      241 cgttttcacg ggaatgacga agagtgtgag aatgacggtt cataaataaa aaaacctgct
      301 taaaacaagc aggtttttga attggcacgc ccacggggat tcgaaccccg gttgccgccg
      361 tgaaagggca gtgtcctagg cctctagacg atgggcgcgt gggaatgggc attatacata
      421 cttctagaaa tttagcaata cgatgccgtc tgaaacttcg tcattcccgc gaaagcggga
      481 atctagaatc tccaagtttt cagacaa
//
LOCUS       DAWFAN010000279          477 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWFAN010000279 DAWFAN010000000
VERSION     DAWFAN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.279"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>477
                     /locus_tag="WGO65_002014"
     CDS             <1..>477
                     /locus_tag="WGO65_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
                     /protein_id="HEZ0328247.1"
                     /translation="NRPAIVDQKSRIGDWEADTIVGKGQKSALLTLVERVTRYTIICK
                     LDSLKAEDTARAAVRALKAHKDRVHTITMDNGKEFYQHTKITKALKAETYFCRPYHSW
                     EKGLNENTNGLIRQYFPKQTDFRNISDREIRRVQDELNHRPRKTLGYETPSVLFLNL"
BASE COUNT          153 a          124 c          106 g           94 t
ORIGIN      
        1 aaccgacccg ctatcgtcga ccagaaatcc cgtatcggcg attgggaagc cgacaccatt
       61 gtcggcaaag gacagaaaag cgcattattg accttggtcg aacgcgttac ccgctacacc
      121 atcatctgca aattggatag cctcaaagcc gaagacactg cccgggcagc tgttagggca
      181 ttaaaggcac ataaagacag ggtgcacacc attaccatgg ataacggcaa agagttctac
      241 caacacacca aaataaccaa agcattgaaa gcggagactt atttttgtcg cccttaccat
      301 tcttgggaga aagggctgaa tgagaacacc aacggactca tccggcaata cttccccaaa
      361 caaaccgatt tccgtaacat cagtgatcgg gagatacgca gggttcaaga tgagttgaac
      421 caccgaccaa gaaaaacact tggctacgaa acgccaagtg ttttattctt gaatctg
//
LOCUS       DAWFAN010000280          446 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWFAN010000280 DAWFAN010000000
VERSION     DAWFAN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.280"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT          160 a          131 c           89 g           66 t
ORIGIN      
        1 aatccataga aaaaccacag gaatctatca gcaaaaacag aaacccccac cgcgtcattc
       61 ccgcaaaagc gggaatccag aaacacaacg cggcaggact ttatcggaaa aaacagaaac
      121 ccccaccacc gtcattcccg cgcaggcggg aatccagacc cgtcggcacg gaaacttacc
      181 ggataaaaca gtttccttag attccacgtc ccagattccc gccttcgcgg gaatgacgag
      241 attttagatt atgggaattt atcaggaatg attgaatcca tagaaaaatc acaggaatct
      301 atcagaaaaa acagaaaccc ccaccgcgtc attcccgcaa aagcgggaat ccaaaaacgc
      361 aacgcaacag gaatctatcg gaaaaaacag aaaccccacc gccgtcattc ccgcaaaagc
      421 gggaatccag acccgtcggc acggaa
//
LOCUS       DAWFAN010000281          433 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.281, whole genome shotgun sequence.
ACCESSION   DAWFAN010000281 DAWFAN010000000
VERSION     DAWFAN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.281"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..>433)
                     /locus_tag="WGO65_002015"
     rRNA            complement(<1..>433)
                     /locus_tag="WGO65_002015"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           85 a          145 c           92 g          111 t
ORIGIN      
        1 atcggtattc ctccacatct ctacgcattt cactgctaca cgtggaattc tacctccctc
       61 tgacacactc gagtcaccca gttcagaacg cagttcccgg gttgagcccg gggatttcac
      121 atcctgctta agtaaccgtc tgcgcccgct ttacgcccag taattccgat taacgctcgc
      181 accctacgta ttaccgcggc tgctggcacg tagttagccg gtgcttattc ttcaggtacc
      241 gtcatcagcc gctgatatta gcaacagcct tttcttccct gacaaaagtc ctttacaacc
      301 cgaaggcctt cttcagacac gcggcatggc tggatcaggc ttgcgcccat tgtccaaaat
      361 tccccactgc tgcctcccgt aggagtctgg gccgtgtctc agtcccagtg tggcggatca
      421 tcctctcaga ccc
//
LOCUS       DAWFAN010000282          433 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.282, whole genome shotgun sequence.
ACCESSION   DAWFAN010000282 DAWFAN010000000
VERSION     DAWFAN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.282"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..256
                     /locus_tag="WGO65_002016"
     CDS             <1..256
                     /locus_tag="WGO65_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0328248.1"
                     /translation="RTDAKADTVAAAAKTADNINTKHLPSTCRDASSASRTKTPGFAF
                     KYFQKISRYRVLPESRQMTKKLRHSRAGGNLGLSAQKLIG"
     gene            302..>433
                     /locus_tag="WGO65_002017"
     CDS             302..>433
                     /locus_tag="WGO65_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0328249.1"
                     /translation="MTGFNDAAGVASASDIKGKYVKSVEVKNGVVTATMASSNVNNEI
                     "
BASE COUNT          124 a          133 c           94 g           82 t
ORIGIN      
        1 gcgcaccgac gccaaagccg acaccgtcgc cgccgccgcc aagaccgccg acaacatcaa
       61 caccaagcac ctgccgtcaa cctgccgcga cgcttcatct gccagccgta caaaaacacc
      121 cggtttcgct ttcaaatact tccaaaaaat cagccgttac cgagtattgc ccgaatcacg
      181 gcaaatgact aaaaaacttc gtcattcccg cgcaggcggg aatctaggtt tgtcggcgca
      241 gaaacttatc gggtaaaaag gtttctccag tcctgaatcc tagattccca ctttcgtggg
      301 aatgacggga tttaatgatg ccgccggcgt ggcatccgcc tccgacatca aaggcaaata
      361 tgttaaaagc gttgaagtca aaaacggcgt cgttaccgcc acaatggctt caagcaacgt
      421 aaacaacgaa atc
//
LOCUS       DAWFAN010000283          430 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.283, whole genome shotgun sequence.
ACCESSION   DAWFAN010000283 DAWFAN010000000
VERSION     DAWFAN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.283"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           84 a           96 c          126 g          124 t
ORIGIN      
        1 gggatttagg gctaatctag tacagcccct tagatttaat tcactatacc tgcgtaatgg
       61 tatcgggtac tcatcatcaa cctgccttgc attcggttgt gttgtccggc aactgtccgg
      121 ttgtgtcgat gggttgccct gccgctccct gtgctgcgca acggttgtgt gccttgggtg
      181 ggcgcgggtg gcggccgggc ggtttgttgc tatgatacgg gagttccgat atacaagggt
      241 gggcttcagc ccaccaatca acgaccgaaa aagaaaaaac gttgggctga agcccactct
      301 acgcaattac gcaactgtta tcgtggggat gacgggattt taggtttttg tttttctgtt
      361 tttgcgggaa ggatgggttt taggttgcgg tcatttatcg gaaaagcaga aatcgctccg
      421 ccgtcattcc
//
LOCUS       DAWFAN010000284          430 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.284, whole genome shotgun sequence.
ACCESSION   DAWFAN010000284 DAWFAN010000000
VERSION     DAWFAN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.284"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT          125 a          106 c          116 g           83 t
ORIGIN      
        1 tcgcgggaat gacggcgcat aagttcccgt gcggacagac ctagattccc acctgcgtgg
       61 gaatgacgat gcagaagttg cccgaaactc aaaaaaaacg aaaccgaacg aaccggattc
      121 ccgctttcgc gggaatgacg gcgcataagt tcccgtgcgg acagacctag attcccacct
      181 gtgtgggaat gacgattcag aagttgcccg aaaccaaaaa actgaagccg aacggtctgg
      241 attcccgctt tcgcgggaat gacggcatat cagtttctgt gcggacaaac ctggattccc
      301 atctgcgtag gaatgacgat gcagaagttg cccgaaactc aaaaaaaacg aaaccgaacg
      361 aaccggattc ccgcttttac gggaatgacg ggattttggg ttgtggtaat ttatcgggaa
      421 aacggaaacc
//
LOCUS       DAWFAN010000285          424 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.285, whole genome shotgun sequence.
ACCESSION   DAWFAN010000285 DAWFAN010000000
VERSION     DAWFAN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.285"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(14..226)
                     /locus_tag="WGO65_002018"
     CDS             complement(14..226)
                     /locus_tag="WGO65_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ0328250.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
BASE COUNT           87 a          119 c          112 g          106 t
ORIGIN      
        1 catttttgcc gctttatttc aacgcggcat catagccgcc gtcttcaacg gcttcaatca
       61 acgcccctgc atcggtttgc gcggggtcgt atccgacggt cgcgcttttg ttttcaaggc
      121 tgacttcgac gcttgccacg ccttttacgc cttccaatat ccgggtaacg cttttgacgc
      181 agccgccgca gctcatgccg ccgatgtcga ggataagggt ttccatcatt ttcctttcgt
      241 tggaactgca atctgacggg cgttattgta agtcggggcg tgaacttggg caaacgcgga
      301 aacggtgcgg cggtttggaa aaagacggac gcttgcgcat aatggcggca attcccatca
      361 ggacgacaac aatgaacgct tcacaaaaac cctggttgag catcatcgcc ttggcaatcg
      421 gcgc
//
LOCUS       DAWFAN010000286          421 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.286, whole genome shotgun sequence.
ACCESSION   DAWFAN010000286 DAWFAN010000000
VERSION     DAWFAN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.286"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT          122 a           85 c          105 g          109 t
ORIGIN      
        1 gacgggattt caggttgctg tttttgattt tctgtttttg tgggaataac gggactttgg
       61 atgatgtgca tttatcggga aaacaaaaaa ccctccgccg tcattcccgc gaaagcggga
      121 atctagaatt ttaatgcggc aagaatttat caagaaaaac cgaagcttaa agacctagat
      181 tcccgcctgc gcgggaatga cggtttagaa atcacccgaa acgagaaaaa gaaccgaaac
      241 cgaacaagcc ggattcccgc acaggcggga aggacggaat ttcagattgt tgtttttgat
      301 tttctgtttt tgtgggaata acgggacttt ggatgatgtg catttatcga gaaaactaaa
      361 aaccctccgc cgtcattccc gcgaaagcgg gaatctagac ctttcggtgc ggaaacttat
      421 c
//
LOCUS       DAWFAN010000287          417 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.287, whole genome shotgun sequence.
ACCESSION   DAWFAN010000287 DAWFAN010000000
VERSION     DAWFAN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.287"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>417
                     /locus_tag="WGO65_002019"
     CDS             <1..>417
                     /locus_tag="WGO65_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0328251.1"
                     /translation="GQDAYSINLIEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGL
                     DSVDDIVIRKDACHLNQDLGKSLLDLGFKKIPSVYPEYEAYEDKRSIPENPYIHELQY
                     IKKGENPAIITHQNYHRYGENDYSTDIGSCINGFTV"
BASE COUNT          140 a           73 c           85 g          119 t
ORIGIN      
        1 ccgggcagga tgcttactcc attaatttga tagagattag cgtctattac aaaaaaaccg
       61 accaaggttg ggttcttgag ccatacaacc agcaaaacaa agcacacttt attcaatttc
      121 tacgcgatgg tttggatagc gtggacgata ttgttatccg aaaagatgcg tgtcatttga
      181 atcaagactt aggaaaatcg ttgcttgatt tgggatttaa aaaaatcccg tctgtctatc
      241 ctgaatatga agcttatgaa gataaaaggt cgattcctga aaacccatat attcatgaac
      301 tacagtatat taagaaagga gaaaatccgg cgattattac tcatcagaac tatcataggt
      361 atggagagaa cgattacagt actgatatag gttcctgtat taacggtttc acggtac
//
LOCUS       DAWFAN010000288          405 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.288, whole genome shotgun sequence.
ACCESSION   DAWFAN010000288 DAWFAN010000000
VERSION     DAWFAN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.288"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(178..>405)
                     /locus_tag="WGO65_002020"
     CDS             complement(178..>405)
                     /locus_tag="WGO65_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328252.1"
                     /translation="NVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSM
                     SVFGSNTAATGGNVDANTQSVQQPLLVTPSA"
BASE COUNT          131 a           80 c           78 g          116 t
ORIGIN      
        1 atagtacggt aaggcgaggc aacgctgtac tggtttttgt gaatccacta tattgctaga
       61 agtgcaccga ttaaggttaa ttaaaatacg acccttatcc caatattaag aaaaacagca
      121 atcaaaataa acttgattgc tgtttttcag tttaagccat gaatgtgatt aggctcctta
      181 tgcagatggc gttaccaata acggctgctg tacggattgt gtattggcat ccacatttcc
      241 tcctgtcgca gcagtattag aaccgaacac agacatagac tgtaccaaat tattgccagc
      301 attaatatac ttggcaaaat ctggattact gatagctgca tcatcaaaga caaatgtatc
      361 tacacgatgg ttttcaccat agaagaatcc ggaaatacgt acgtt
//
LOCUS       DAWFAN010000289          400 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.289, whole genome shotgun sequence.
ACCESSION   DAWFAN010000289 DAWFAN010000000
VERSION     DAWFAN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.289"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            108..323
                     /gene="rpmE"
                     /locus_tag="WGO65_002021"
     CDS             108..323
                     /gene="rpmE"
                     /locus_tag="WGO65_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ0328253.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT          124 a          104 c           74 g           98 t
ORIGIN      
        1 attttatcga atatcctctt gacgaaacaa cagaaaatag cgagaatgcc gagcttgtct
       61 gaagcatagg tacatcccct atgcccgctt ttttgaaagg aaatcaaatg aaacaaggta
      121 ttcacccgaa ctaccacgaa gtcaacgtta cctgctcttg cggcaacaaa ttcgcaacca
      181 aatccgcgat ggaaaaagaa aacttcaata tcgaggtttg ctccctgtgc cacccgttct
      241 ataccggcac ccaaaaaatc gtcgatacca ccggccgcgt ggacaaattc aacaacaaat
      301 tcggcaacct gttcaaacgc tgattgccgt tttgcaagaa ttaagccctg ccatcatttc
      361 gatggcaggg ctttttaata tttggcacac tgacttctta
//
LOCUS       DAWFAN010000290          382 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.290, whole genome shotgun sequence.
ACCESSION   DAWFAN010000290 DAWFAN010000000
VERSION     DAWFAN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.290"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..306
                     /locus_tag="WGO65_002022"
     CDS             <1..306
                     /locus_tag="WGO65_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328254.1"
                     /translation="WDDEEYWKLENALIEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQ
                     NWKLFEIKASPWLPKSVKINERYERFRVMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
BASE COUNT          129 a           40 c           80 g          133 t
ORIGIN      
        1 tgggatgatg aagaatattg gaaattggag aatgctttaa tcgaggttag aaaaaaatat
       61 ccttatccga tggatatacc aagggacatc gtgattggaa tcggtaccat tattgatttt
      121 ttaatggttc aaaattggaa actttttgaa attaaagctt ctccttggtt gcctaaaagt
      181 gtcaaaatta atgagcgtta tgaaaggttc agagtaatgc tccgttatat ttttactgat
      241 ctagatgtag aggactggaa atttttttac tttccaatac aacatagtaa aggtagattg
      301 agatgaaaag tttaattata ttggagatac attattatgt gtgagttcaa ggatattata
      361 agaaacgttc cttattttga gg
//
LOCUS       DAWFAN010000291          376 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.291, whole genome shotgun sequence.
ACCESSION   DAWFAN010000291 DAWFAN010000000
VERSION     DAWFAN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.291"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..185)
                     /locus_tag="WGO65_002023"
     CDS             complement(<1..185)
                     /locus_tag="WGO65_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328255.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAAR"
     gene            complement(182..>376)
                     /locus_tag="WGO65_002024"
     CDS             complement(182..>376)
                     /locus_tag="WGO65_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0328256.1"
                     /translation="RGEDGFKPDNAGHHSRLAYAQAGGIVEPFFISNDTDLALFKTTK
                     WGICRAIADAIAMKLGAARV"
BASE COUNT           87 a           99 c           89 g          101 t
ORIGIN      
        1 cgcgccgccc gttttgcggc tgccacgcct gagtttctta aatttgggcg tggataggtg
       61 tgtatcggac gaacatttgg catctgtata agtgcgcggt ttacctgctc attactagga
      121 ttttctccaa tcaattttgt aagaaaatag gaagcagctt ctttcaattt accaataata
      181 ttcatactct tgccgccccc aatttcatcg caatcgcgtc cgcaatcgcg cggcagatgc
      241 cccatttggt cgtcttaaac aaggccaaat cagtgtcgtt gctgatgaaa aaaggctcaa
      301 caatgccgcc agcctgcgca taagccaggc gcgaatgatg gcccgcattg tcgggtttaa
      361 agccgtcttc gccgcg
//
LOCUS       DAWFAN010000292          366 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.292, whole genome shotgun sequence.
ACCESSION   DAWFAN010000292 DAWFAN010000000
VERSION     DAWFAN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.292"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>366
                     /locus_tag="WGO65_002025"
     CDS             <1..>366
                     /locus_tag="WGO65_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0328257.1"
                     /translation="YRNRLAQDDTLSELLKLINRQLTEKGLKIEKASAAVIDATIIQT
                     AGSKQRQAIEVDEEGQISGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKLH
                     ITPANAHECKHLPPLLEGIA"
BASE COUNT          121 a          105 c           83 g           57 t
ORIGIN      
        1 taccgcaacc ggctggcgca agacgacacc ctgtccgaat tgctcaaact gattaaccga
       61 caactgaccg aaaaaggttt aaaaatagag aaagcatccg ccgccgtcat tgacgccacc
      121 attattcaga ccgctggcag caaacagcgt caggccatag aagttgacga agaaggacaa
      181 atcagcggcc aaaccacacc gagtaaggac agcgatgccc gttggataaa gaaaaacggc
      241 ctctacaaac tcggttacaa acaacatacc cgtaccgatg cggaaggcta tatcgagaaa
      301 ctgcacatca cccccgccaa tgcccatgag tgcaaacacc tgccgccgtt gttagaaggg
      361 atagct
//
LOCUS       DAWFAN010000293          353 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.293, whole genome shotgun sequence.
ACCESSION   DAWFAN010000293 DAWFAN010000000
VERSION     DAWFAN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.293"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           92 a           92 c           70 g           99 t
ORIGIN      
        1 tcgttcccac ggtcaggatt tcagattgcg gacatctgtc agaaaagaca aaaaaccttc
       61 cgccgtcatt ccctacaggc gagaatccga tccgttgaaa ttcggttgtt ttaaataaat
      121 tcttgcagct ttgattttct gtttttccga taacgccgta actttgaaac gcgaaagcgg
      181 taatccgatc cgttgggatt ttgcaacttc aaatcaatcc gcaaactgaa atcccgtcat
      241 tcccgcgcag tcgtgaatcc gaacgcgtcc gcacgaaaac ctgcatcccg tcattcccac
      301 ggaagtggga atccagtttt ttgagtttca gtcattcccg ataaattgcc tta
//
LOCUS       DAWFAN010000294          351 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.294, whole genome shotgun sequence.
ACCESSION   DAWFAN010000294 DAWFAN010000000
VERSION     DAWFAN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.294"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            26..>351
                     /locus_tag="WGO65_002026"
     CDS             26..>351
                     /locus_tag="WGO65_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0328258.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNSAAGKAATGIEALSTVKNKRWCQ
                     VLSKAVA"
BASE COUNT           91 a           81 c          105 g           74 t
ORIGIN      
        1 aatcaatatt ttttggagat gatgaatggg caaaaccgta accttaaccg ctggacacag
       61 caacaccgac ccgggtgcgg tcaacggcag cgaccgtgag gcggacttgg cgcaggatat
      121 gcgcaacatc gtggctgcta ttttgcgcga tgactacggt ttgactgtta aaaccgacgg
      181 cacaggcaaa ggcaatatgc cgctgcgtga agctgtaaaa ctgattcgcg gctcggatgt
      241 ggcgattgag ttccatacca actcggcggc cggtaaagca gctacgggca ttgaggcgtt
      301 gagtaccgtt aaaaacaaac gctggtgtca ggtgttgagc aaagctgttg c
//
LOCUS       DAWFAN010000295          346 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.295, whole genome shotgun sequence.
ACCESSION   DAWFAN010000295 DAWFAN010000000
VERSION     DAWFAN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.295"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..152)
                     /locus_tag="WGO65_002027"
     CDS             complement(<1..152)
                     /locus_tag="WGO65_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328259.1"
                     /translation="MKKSLIALALSVLKPQMPEFEIKPASIGYLKQHPSMRLGKSGVA
                     AAKRAAR"
     gene            complement(149..>346)
                     /locus_tag="WGO65_002028"
     CDS             complement(149..>346)
                     /locus_tag="WGO65_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0328260.1"
                     /translation="RGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAAKV"
BASE COUNT           85 a          104 c           81 g           76 t
ORIGIN      
        1 cgcgccgcgc gtttcgcggc tgccacgccc gacttgccta ggcgcataga cggatgttgt
       61 ttcaaatagc caatactggc aggcttaatc tcaaattcag gcatctgcgg tttcaagaca
      121 gacagagcca aagcaatcaa agactttttc ataccttcgc cgctcccaat tccatcgcaa
      181 tcgcgtccgc aatcgcgcgg cagatgcccc atttagtagt cttaaacaag gccaaatcag
      241 tgtcgttgct gatgaaaaaa ggctcaaaca caatgccacc tgcctgcgca taagccaggc
      301 gcgaatgctg gcccgcattg tctggtttaa agccgtcttc gccgcg
//
LOCUS       DAWFAN010000296          338 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.296, whole genome shotgun sequence.
ACCESSION   DAWFAN010000296 DAWFAN010000000
VERSION     DAWFAN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.296"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           91 a           82 c           89 g           76 t
ORIGIN      
        1 cccctctccc tgtgggagag ggttagggag agggcagtaa gccgtaggct tacatcaaag
       61 tcgataacgc tttcacaaag cctgtgtgca ctgaaaactg aaaggtcatc tgaaaacgga
      121 gctttcgaag gaagcagagt ttctgcgaag ctaaaaccga agctgcaaaa aatcgaaaag
      181 cagcctgcac gttgaaaatg ccgcccaagc aaacctgcgg tttgccgccc tctccctagc
      241 cctctcccac agggagaggg gattgggttg caggctgcct ttaaggttta ggcaaatttt
      301 taacttcgtt gaagctgcga tttcagaagc tccgtttt
//
LOCUS       DAWFAN010000297          331 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.297, whole genome shotgun sequence.
ACCESSION   DAWFAN010000297 DAWFAN010000000
VERSION     DAWFAN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.297"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            41..331
                     /locus_tag="WGO65_002029"
                     /pseudo
     CDS             41..331
                     /locus_tag="WGO65_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          103 a           93 c           71 g           64 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat actttatgcc gctaccgcaa
       61 ctggcggctc aagacaatac cctgtccgaa ttgctcaaac tgattaaccg acaactgacc
      121 gaaaaaggcc taaaagtaga gaaagcatcc gccgccgttg ttgacgccac cattattcag
      181 accgccggca gcaaacagcg tcaggctata gaagtcgatg aagaaggaca agtcagcggc
      241 caaaccacac cgagtaaaga cagcgatgcc cgttggataa agaaaaacgg cctctacaaa
      301 ctcggttaca aacaacatac ccgtaccgat g
//
LOCUS       DAWFAN010000298          325 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.298, whole genome shotgun sequence.
ACCESSION   DAWFAN010000298 DAWFAN010000000
VERSION     DAWFAN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.298"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>325
                     /locus_tag="WGO65_002030"
     CDS             <1..>325
                     /locus_tag="WGO65_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328261.1"
                     /translation="KSQIKFQSSNNLKSSGFKLDSSSKSFAKFIEAANFEHIPTGAKA
                     RINAKITASVSRAGVLAGVGLGAKFSTKAVPYVGTALLAHDVYETFKEDIQAQGYQYD
                     PETDKF"
BASE COUNT          111 a           77 c           66 g           71 t
ORIGIN      
        1 aaatcacaaa ttaaatttca atcaagtaat aatttaaaat caagtggttt taaattagat
       61 tcaagttcta agagttttgc caaattcata gaagcggcaa attttgagca catccctacg
      121 ggcgcaaaag cccgaatcaa cgcaaaaata accgccagcg tatcccgcgc cggcgtattg
      181 gcgggggtcg gcttaggcgc gaaattcagc acaaaggcgg ttccctatgt cggaacagcc
      241 cttttagccc atgacgtata cgaaactttc aaagaagaca tacaggcaca aggctaccaa
      301 tacgaccccg aaaccgacaa atttg
//
LOCUS       DAWFAN010000299          320 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.299, whole genome shotgun sequence.
ACCESSION   DAWFAN010000299 DAWFAN010000000
VERSION     DAWFAN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.299"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>320
                     /locus_tag="WGO65_002031"
     CDS             <1..>320
                     /locus_tag="WGO65_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0328262.1"
                     /translation="TPANAHECKHLLPLLEGIAEGTTVYADKGYDSKENRQHLKEHQL
                     LDGIMRKACRNRPLSETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLLKVSA
                     QSHL"
BASE COUNT           87 a           89 c           83 g           61 t
ORIGIN      
        1 actcccgcca atgcccatga gtgcaaacac ctgttgcctt tgctggaagg tatagccgaa
       61 ggcacgaccg tctatgccga caaaggctac gacagtaagg aaaaccggca gcatctgaaa
      121 gaacatcagt tactggacgg cattatgcgc aaagcctgcc gcaaccgtcc gctgtcggaa
      181 acgcaaacca aacgcaaccg gtatttgtcg aagacccgtt atgtggttga acagagcttc
      241 ggtacgctgc accgtaaatt ccgctatgcg cgggcagcct atttcgggct gctcaaagtg
      301 agtgcgcaaa gccatctgaa
//
LOCUS       DAWFAN010000300          318 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.300, whole genome shotgun sequence.
ACCESSION   DAWFAN010000300 DAWFAN010000000
VERSION     DAWFAN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.300"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           90 a           88 c           66 g           74 t
ORIGIN      
        1 acggaaacct gcaccgcgtc attcccacga acccacatcc cgtcattacc acgaaagcgg
       61 gaatctagga cgcagggtta agaaaacctg catcccgtca ttcctcaaaa acagaaaacc
      121 aaaatcagaa acctaaaatc ccgtcattcc cgcgcaggcg ggaatctagg tctgtcagtg
      181 caggaactta tcggataaaa cggtttcttg agattttaca tcctggattc ccactttcgt
      241 gggaatctag aatctcggac tttcagataa tctttgaata ttgccgctgc cttaaggtct
      301 agattcccgc ctgcgcgg
//
LOCUS       DAWFAN010000301          315 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.301, whole genome shotgun sequence.
ACCESSION   DAWFAN010000301 DAWFAN010000000
VERSION     DAWFAN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.301"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT          110 a           75 c           60 g           70 t
ORIGIN      
        1 actcattagt tacctaaaac ttaaaaaaca gaaaccttta cgccgtcatt cccacgaaag
       61 tgggaatcta gaacccaaat gctaaggcga tttatcggaa acggctgaaa ccgaacgaat
      121 tggattcccg cctgcgcagg aatgacaact cattagttac ctaaaactta aaaaacagaa
      181 acctttacac cgtcattccc acgaaagtgg gaatctagaa cccaaatgct aaggcgattt
      241 atcggaaacg gctgaaaccg aatgaattgg attcccgcct gtgcgggaat gacgacttat
      301 tagttaccta acact
//
LOCUS       DAWFAN010000302          308 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.302, whole genome shotgun sequence.
ACCESSION   DAWFAN010000302 DAWFAN010000000
VERSION     DAWFAN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.302"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            18..212
                     /gene="thiS"
                     /locus_tag="WGO65_002032"
     CDS             18..212
                     /gene="thiS"
                     /locus_tag="WGO65_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ0328263.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT           85 a           90 c           77 g           56 t
ORIGIN      
        1 agaaacatcg agaaaccatg aacatcatct taaacggcgg acccgccgaa cttcacggca
       61 caagcgttgc cgacctcatc gcccaaaccg cgccgcaaaa gccctttgcc gtggcggtca
      121 acaccgtttt cgtccccaaa ggcgcgtatg cggaaacggt tttaaacgaa aacgacaaaa
      181 tcgatatcgt gcggccggtg gtcggcggct aggcggtttt gccttttcag acgacccctg
      241 tccccaaaac aacgttatgg tggattaact ttaaatcagg acaaggcgac gaagccgcag
      301 acagtacg
//
LOCUS       DAWFAN010000303          296 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.303, whole genome shotgun sequence.
ACCESSION   DAWFAN010000303 DAWFAN010000000
VERSION     DAWFAN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.303"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           76 a           68 c           92 g           60 t
ORIGIN      
        1 cggaaaaaac agaaaccgct ccgccgtcat tcccgcgcag gcgggaatct aggacgtaaa
       61 atctcaagaa accgttgtac ccgataagtt tccgtgccga caagtccgga ttcccgcctg
      121 cgcgggaatg acggttcagt tgcgtaggac tggattgtga aaaggggcgg attcggtgaa
      181 aacggcggaa atgtgggatt gatggaatcg gtgggctgaa gccctcccta cagagctttc
      241 agacggtatt gtttgcgttt tcgggatggg ggcaaatgaa acgccgacaa accgat
//
LOCUS       DAWFAN010000304          287 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.304, whole genome shotgun sequence.
ACCESSION   DAWFAN010000304 DAWFAN010000000
VERSION     DAWFAN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.304"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            117..>287
                     /locus_tag="WGO65_002033"
     rRNA            117..>287
                     /locus_tag="WGO65_002033"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           83 a           51 c           82 g           71 t
ORIGIN      
        1 ccgataagtg tgagtgcctt gagcctcaca ctgtttgaaa gacagacaag ataatgtttt
       61 gaacattgtc ctgttggttt ctttgaagca gaccagaagt taaaaagtta gagattgaac
      121 ataagagttt gatcctggct cagattgaac gctggcggca tgctttacac atgcaagtcg
      181 gacggcagca cagagaagct tgcttcttgg gtggcgagtg gcgaacgggt gagtaacata
      241 tcggaacgta ccgagtagtg ggggataact gatcgaaaga tcagcta
//
LOCUS       DAWFAN010000305          279 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.305, whole genome shotgun sequence.
ACCESSION   DAWFAN010000305 DAWFAN010000000
VERSION     DAWFAN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.305"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..274
                     /locus_tag="WGO65_002034"
     CDS             <1..274
                     /locus_tag="WGO65_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328264.1"
                     /translation="EYWKLENDLIEVRKKYPYPMDIPRDIVIGISTIIDFLMVPNWEL
                     FEIKASPWLPDSVGIHERYERFTTMLRYIFTEKDIVNVRFDYYNKK"
BASE COUNT           98 a           41 c           54 g           86 t
ORIGIN      
        1 agaatattgg aaattggaga atgatttaat cgaggttaga aaaaaatatc cttatccgat
       61 ggatatacca agggatattg tgattggaat cagtaccatt attgattttt taatggttcc
      121 aaattgggag cttttcgaaa ttaaagcttc cccttggcta cctgatagcg tgggaattca
      181 tgaacgttat gaaagattca caacaatgct ccgttatatt tttaccgaga aagacatagt
      241 caacgtgcga tttgattatt acaacaaaaa atagcaaac
//
LOCUS       DAWFAN010000306          278 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.306, whole genome shotgun sequence.
ACCESSION   DAWFAN010000306 DAWFAN010000000
VERSION     DAWFAN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.306"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..249
                     /locus_tag="WGO65_002035"
     rRNA            <1..249
                     /locus_tag="WGO65_002035"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           69 a           67 c           83 g           59 t
ORIGIN      
        1 atcgtagtcc ggattgcact ctgcaactcg agtgcatgaa gtcggaatcg ctagtaatcg
       61 caggtcagca tactgcggtg aatacgttcc cgggtcttgt acacaccgcc cgtcacacca
      121 tgggagtggg ggataccaga agtaggtagg gtaaccgcaa ggagcccgct taccacggta
      181 tgcttcatga ctggggtgaa gtcgtaacaa ggtagccgta ggggaacctg cggctggatc
      241 acctcctttc tagagaaaga agaggcttta ggcattca
//
LOCUS       DAWFAN010000307          276 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.307, whole genome shotgun sequence.
ACCESSION   DAWFAN010000307 DAWFAN010000000
VERSION     DAWFAN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.307"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           92 a           65 c           65 g           54 t
ORIGIN      
        1 tggatggatt cgtcgttccc gcgaaagcgg gaatccggaa ccagaaacca caaaaattta
       61 tcgggaaaac cgaaactcaa aaacaacgga aaccgaacgg acaggattcc cgcctgcgcg
      121 ggaatgacgg ctcatacatc acccaaaaaa ttgaaaccaa acagatggga ttccctcttg
      181 cgtgaggctg acagatgccg tctgaaagac tttcagacgg catagctttt tctctttgaa
      241 tttatagtgg attaacaaaa atcaggacaa ggcggc
//
LOCUS       DAWFAN010000308          269 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.308, whole genome shotgun sequence.
ACCESSION   DAWFAN010000308 DAWFAN010000000
VERSION     DAWFAN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.308"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           71 a           55 c           78 g           65 t
ORIGIN      
        1 atgtaggttc gcgggaatga cgcggtgcag gtttccgtat ggatggattc gtcattcccg
       61 cgcaggcggg aatctagacc ttgggataac agcaatattc aaagattata aaagacccgt
      121 cattcccgcg caggcgggaa tctagacctt agaataacag caatattcaa agattatctg
      181 aaagtccgag attctagatt cccactttcg tgggaatgac ggaatgtagg ttcgcgggaa
      241 tgacgcggtg caggtttccg tgcggatgg
//
LOCUS       DAWFAN010000309          269 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.309, whole genome shotgun sequence.
ACCESSION   DAWFAN010000309 DAWFAN010000000
VERSION     DAWFAN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.309"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           59 a           50 c           81 g           79 t
ORIGIN      
        1 atttatcgga aatgaccgaa actgaacgga ctggattccc gcttttgcgg gaatgacggt
       61 tcagttgcta cggttattgt caggtttcgg ttatgttgga atttcgggaa acttatgaat
      121 cgtcattccc gcgcaggcgg gaatctagac cggtcggtgc ggaaacttat cgggaaaaaa
      181 ggtttcttga gattttacgt cctggattcc cactttcgtg ggaatgacgt ggtgcaggtt
      241 cgtaggaatg acgtggtgca ggtttccgt
//
LOCUS       DAWFAN010000310          267 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.310, whole genome shotgun sequence.
ACCESSION   DAWFAN010000310 DAWFAN010000000
VERSION     DAWFAN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.310"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            41..211
                     /locus_tag="WGO65_002036"
     CDS             41..211
                     /locus_tag="WGO65_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ0328265.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT           71 a           71 c           71 g           54 t
ORIGIN      
        1 ggtttttgtt catccgctat acaaaaacaa ggaaacacaa atggcgcaat atatgtgcgg
       61 cccctgcggc tggatttacg atgaagaaca cggcgaccct gaacacggca tcccccccgg
      121 gacaaagttt gaagacattc ccgacgactg gaaatgcccc gaatgcggcg tgggcaaaga
      181 agatttctac ctgttggatt tcgtgatata gcggcagccg aaccccttcg gaaaattcct
      241 cggcgttccg cctgcggtag cagggaa
//
LOCUS       DAWFAN010000311          255 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.311, whole genome shotgun sequence.
ACCESSION   DAWFAN010000311 DAWFAN010000000
VERSION     DAWFAN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.311"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           70 a           61 c           60 g           64 t
ORIGIN      
        1 cgcttgttcg gtaaatgaga gggcggatta cgcgcctgtc agataaaccc cgtgtttaaa
       61 cgggcggcaa tgaggtacgc gtagagcctt gaagcgcaat cgatatatta tttccaccaa
      121 aacggacgac cccgcctgcc ttgcaaaccc ttaaaatgaa gccacccgga ttaatccgag
      181 tggccgtgga aaatcactta ccgcttgatt tatttaaaat ttatggtata atttacctta
      241 gctggcagat gctgc
//
LOCUS       DAWFAN010000312          253 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.312, whole genome shotgun sequence.
ACCESSION   DAWFAN010000312 DAWFAN010000000
VERSION     DAWFAN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.312"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(20..>253)
                     /locus_tag="WGO65_002037"
     CDS             complement(20..>253)
                     /locus_tag="WGO65_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0328266.1"
                     /translation="LIAQKEFEEFVNNVKEYDILESFSYFLNKRVAHNIWKIYFSDES
                     VIFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
BASE COUNT           97 a           42 c           26 g           88 t
ORIGIN      
        1 tacaggttca gaataagaat tactcgaaca tagaattaaa atcagaacta tcggtgtact
       61 cttgataaac aaattcatga acagtttttc cattctcttc tgattttctt ataaaaataa
      121 cagattcatc agaaaaataa atcttccaaa tattatgtgc gactctctta tttaaaaaat
      181 aagagaagct ctctaaaata tcgtactctt ttacgttatt aacaaattct tcaaattctt
      241 tctgagcaat taa
//
LOCUS       DAWFAN010000313          252 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.313, whole genome shotgun sequence.
ACCESSION   DAWFAN010000313 DAWFAN010000000
VERSION     DAWFAN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.313"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           59 a           51 c           73 g           69 t
ORIGIN      
        1 attcgtcatt cccgcgcagg cgggaatcta gacattcaat gctaaggcaa tttatcggga
       61 atgactgaaa ctcaaaaaaa ctggattccc acttttgtgg gaatggcggg atgtaggttc
      121 gtgggaatga cgggatgcag gtttccgtat ggatggattc gtcattcccg cgcaggcggg
      181 aatccaggtc tgtcggtgcg gaaacttatc gggtaaaacg gtttcttgag attttgcgtc
      241 ttgtattccc ac
//
LOCUS       DAWFAN010000314          250 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.314, whole genome shotgun sequence.
ACCESSION   DAWFAN010000314 DAWFAN010000000
VERSION     DAWFAN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.314"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           56 a           81 c           55 g           58 t
ORIGIN      
        1 aatcttgaaa tccgtcattc ccgcgcaggc gggaatccag acctgtccgc acagaaactt
       61 atcggataaa aacagttgct caaacccctc gttctagatt cccacttccg tgggaatgac
      121 ggttcagttg ctacggcccc gccctacaat acgccttgcg aatctgtccc acgcccgccc
      181 ttgtatgcct caaatgggtt aaactacgca cttcccgact tcccgtttcg ggcaggggta
      241 caggtccgat
//
LOCUS       DAWFAN010000315          250 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.315, whole genome shotgun sequence.
ACCESSION   DAWFAN010000315 DAWFAN010000000
VERSION     DAWFAN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.315"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           75 a           54 c           63 g           58 t
ORIGIN      
        1 gaatgacggg atgtgggttc gtgggaatga cgaagtagaa gttacccgaa atttaaaaca
       61 agcgaaaccg aacgaactgg attcccgctt tcgtgggaat gacggcggag cggtttctgc
      121 ttttcccaat aaatgcccca acctaaaatc cgtcattcct gcgcaggcgg gaatctagac
      181 attcaatgct aaggcaattt atcgggaatg actgaaactc aaaaagctag attcccactt
      241 tcgtgggaat
//
LOCUS       DAWFAN010000316          242 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.316, whole genome shotgun sequence.
ACCESSION   DAWFAN010000316 DAWFAN010000000
VERSION     DAWFAN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.316"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..232)
                     /locus_tag="WGO65_002038"
     CDS             complement(<1..232)
                     /locus_tag="WGO65_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0328267.1"
                     /translation="MYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKEQTSFNNP
                     EPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDN"
BASE COUNT           71 a           46 c           47 g           78 t
ORIGIN      
        1 cattgtcttg cgtataccgt gcaagatagc cataagggat aaccattttg gtgccctgaa
       61 aatcaaatgt aaccgtatgt tcaaatcctg tcattggctc gggattgttg aaactggttt
      121 gttctttagc ctgttgcgca cttactgctt tttgatctgt cttttcattc acaggttcac
      181 atgaggtcat agtaaaaaca ctcccaataa acaaaataaa aagttgataa atggtagtag
      241 ca
//
LOCUS       DAWFAN010000317          239 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.317, whole genome shotgun sequence.
ACCESSION   DAWFAN010000317 DAWFAN010000000
VERSION     DAWFAN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.317"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            36..>239
                     /locus_tag="WGO65_002039"
     CDS             36..>239
                     /locus_tag="WGO65_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0328268.1"
                     /translation="MLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMTRL
                     PTFLCNDVVKNPQVEKYLADYQAH"
BASE COUNT           67 a           51 c           59 g           62 t
ORIGIN      
        1 acggcacagg cggcttgttg caaggcaaaa aacatatgct ttcactgact tggaatgcgc
       61 cgattgaggc gtttacccgc gaaggcgatt tctttgaagg caaaggcgtt gatgttttgt
      121 atatgcactt ccacaaagcc aacgagtttt tgggtatgac ccgcctgccg acattcttat
      181 gtaacgatgt ggttaaaaat ccacaagtgg aaaaatactt ggcagattat caggcacac
//
LOCUS       DAWFAN010000318          237 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.318, whole genome shotgun sequence.
ACCESSION   DAWFAN010000318 DAWFAN010000000
VERSION     DAWFAN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.318"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           80 a           54 c           54 g           49 t
ORIGIN      
        1 acgaaatcga acggactgga ttcccgcctg cgcgggaatg acgagtcaga agttactcga
       61 aacttaaaaa accgaaatcg aacgaattgg atggattccc gcctgcgcgg gaatgacgat
      121 tgatacgttt cgataattta ttgaaaatgg ctgaaatcca aagttacaga aatttataaa
      181 caaaaaacag caacccgctg ccgtcattcc cgcgaaagcg ggaatccagt cccttga
//
LOCUS       DAWFAN010000319          228 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.319, whole genome shotgun sequence.
ACCESSION   DAWFAN010000319 DAWFAN010000000
VERSION     DAWFAN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.319"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           76 a           56 c           62 g           34 t
ORIGIN      
        1 aaaagcaaac accgtccgtc gtgttgccgt ttgcggatga gtacgggtca accccaatgc
       61 cgccgaaacc gtcgaagccg ccttcaacat tgccgccgcc aaagccgcaa agttggcaaa
      121 aacggtaaaa ccggggagat aaaagccgat ggcaggaaag taaatgtgag gatagacagt
      181 acggaggcag acctgcttta tccggcaggg caataagaaa acaaaaat
//
LOCUS       DAWFAN010000320          219 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.320, whole genome shotgun sequence.
ACCESSION   DAWFAN010000320 DAWFAN010000000
VERSION     DAWFAN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.320"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            15..>219
                     /locus_tag="WGO65_002040"
     CDS             15..>219
                     /locus_tag="WGO65_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0328269.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLW"
BASE COUNT           60 a           30 c           61 g           68 t
ORIGIN      
        1 taaaggatct tattatgaat gagggtgaag ttgttttaac accagaacaa atccaaacct
       61 tgcgtggtta tgctttccgt ggcgatacct atggcggttg gcgttatttg gctaatttgg
      121 gtgaccgtta tgcggatgat gctgctgcaa ttgtcggtaa ggatgcaaac ttaaatggtt
      181 tgaatttatg gatgaaaaaa ggtgtggaaa acctatggg
//
LOCUS       DAWFAN010000321          216 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.321, whole genome shotgun sequence.
ACCESSION   DAWFAN010000321 DAWFAN010000000
VERSION     DAWFAN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.321"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>216
                     /locus_tag="WGO65_002041"
     rRNA            <1..>216
                     /locus_tag="WGO65_002041"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           54 c           64 g           50 t
ORIGIN      
        1 gagccgtaac acaggtgctg catggctgtc gtcagctcgt gtcgtgagat gttgggttaa
       61 gtcccgcaac gagcgcaacc cttgtcatta gttgccatca ttcagttggg cactctaatg
      121 agactgccgg tgacaagccg gaggaaggtg gggatgacgt caagtcctca tggcccttat
      181 gaccagggct tcacacgtca tacaatggtc ggtaca
//
LOCUS       DAWFAN010000322          212 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514759 isolate 3255
            SAMEA1972431-rid20301133.denovo.322, whole genome shotgun sequence.
ACCESSION   DAWFAN010000322 DAWFAN010000000
VERSION     DAWFAN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1972431
            Sequence Read Archive: ERR332331
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995174.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:32:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 1,984
            Genes (coding)                    :: 1,898
            CDSs (with protein)               :: 1,898
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 47 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1972431-rid20301133.denovo.322"
                     /strain="2590STDY5514759"
                     /serovar="W"
                     /isolate="3255"
                     /isolation_source="pharynx"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..167
                     /locus_tag="WGO65_002042"
     CDS             <1..167
                     /locus_tag="WGO65_002042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0328270.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
BASE COUNT           63 a           39 c           64 g           46 t
ORIGIN      
        1 tcatgggaca ggttgcgttt tacgaaaaga tgattgggct gtggtcggcc aaaagccgtg
       61 aggcaagcga acaggcagac ttggcggcgt ttgaatttgc ggagggcgaa ctggccaatt
      121 atcaggaaat gctgaaacgg cacctgcaaa ccaaaagtgt ggaatagcaa tgcgtatttt
      181 ggatattttt aaaaacccag cgacaggcaa tg
//
